LOCUS       DAVMAF010000001        68432 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMAF010000001 DAVMAF010000000
VERSION     DAVMAF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.001"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..283
                     /locus_tag="VYO97_000001"
     CDS             <1..283
                     /locus_tag="VYO97_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752901.1"
                     /translation="EKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMDLHEQSK
                     ADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            287..619
                     /locus_tag="VYO97_000002"
     CDS             287..619
                     /locus_tag="VYO97_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1752902.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            623..1579
                     /gene="lytA"
                     /locus_tag="VYO97_000003"
     CDS             623..1579
                     /gene="lytA"
                     /locus_tag="VYO97_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1752903.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1832..2800)
                     /locus_tag="VYO97_000004"
     CDS             complement(1832..2800)
                     /locus_tag="VYO97_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1752904.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            2936..3793
                     /locus_tag="VYO97_000005"
     CDS             2936..3793
                     /locus_tag="VYO97_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1752905.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            3780..4559
                     /locus_tag="VYO97_000006"
     CDS             3780..4559
                     /locus_tag="VYO97_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1752906.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            4583..5935
                     /locus_tag="VYO97_000007"
     CDS             4583..5935
                     /locus_tag="VYO97_000007"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1752907.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKKTGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            6009..6383
                     /locus_tag="VYO97_000008"
     CDS             6009..6383
                     /locus_tag="VYO97_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1752908.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            6385..7233
                     /locus_tag="VYO97_000009"
     CDS             6385..7233
                     /locus_tag="VYO97_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1752909.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            7510..8277
                     /locus_tag="VYO97_000010"
     CDS             7510..8277
                     /locus_tag="VYO97_000010"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1752910.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLACFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRTQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEIRESIQQGAADEEELITGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            8289..9473
                     /locus_tag="VYO97_000011"
     CDS             8289..9473
                     /locus_tag="VYO97_000011"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1752911.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKDIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            9470..11341
                     /locus_tag="VYO97_000012"
     CDS             9470..11341
                     /locus_tag="VYO97_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1752912.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(11484..12668)
                     /gene="mntE"
                     /locus_tag="VYO97_000013"
     CDS             complement(11484..12668)
                     /gene="mntE"
                     /locus_tag="VYO97_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1752913.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            12972..15668
                     /locus_tag="VYO97_000014"
     CDS             12972..15668
                     /locus_tag="VYO97_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1752914.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            15681..16472
                     /gene="yghU"
                     /locus_tag="VYO97_000015"
     CDS             15681..16472
                     /gene="yghU"
                     /locus_tag="VYO97_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1752915.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            16588..16998
                     /locus_tag="VYO97_000016"
     CDS             16588..16998
                     /locus_tag="VYO97_000016"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1752916.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            17010..18605
                     /locus_tag="VYO97_000017"
     CDS             17010..18605
                     /locus_tag="VYO97_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752917.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            18672..18836
                     /locus_tag="VYO97_000018"
     CDS             18672..18836
                     /locus_tag="VYO97_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1752918.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            18915..19643
                     /gene="yaaA"
                     /locus_tag="VYO97_000019"
     CDS             18915..19643
                     /gene="yaaA"
                     /locus_tag="VYO97_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1752919.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(19920..20468)
                     /locus_tag="VYO97_000020"
     CDS             complement(19920..20468)
                     /locus_tag="VYO97_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1752920.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLAADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLIEAIK"
     gene            20789..21277
                     /locus_tag="VYO97_000021"
     CDS             20789..21277
                     /locus_tag="VYO97_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1752921.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            21274..22461
                     /locus_tag="VYO97_000022"
     CDS             21274..22461
                     /locus_tag="VYO97_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1752922.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVAPNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEKRLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            22454..22819
                     /locus_tag="VYO97_000023"
     CDS             22454..22819
                     /locus_tag="VYO97_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1752923.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            22835..24181
                     /gene="asnS"
                     /locus_tag="VYO97_000024"
     CDS             22835..24181
                     /gene="asnS"
                     /locus_tag="VYO97_000024"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1752924.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            24334..24624
                     /locus_tag="VYO97_000025"
     CDS             24334..24624
                     /locus_tag="VYO97_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1752925.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            24636..25106
                     /gene="ssbA"
                     /locus_tag="VYO97_000026"
     CDS             24636..25106
                     /gene="ssbA"
                     /locus_tag="VYO97_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1752926.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            25138..25377
                     /locus_tag="VYO97_000027"
     CDS             25138..25377
                     /locus_tag="VYO97_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1752927.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            25672..27072
                     /locus_tag="VYO97_000028"
     CDS             25672..27072
                     /locus_tag="VYO97_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1752928.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            27074..27433
                     /locus_tag="VYO97_000029"
     CDS             27074..27433
                     /locus_tag="VYO97_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1752929.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            27487..28236
                     /locus_tag="VYO97_000030"
     CDS             27487..28236
                     /locus_tag="VYO97_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1752930.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            28226..28525
                     /locus_tag="VYO97_000031"
     CDS             28226..28525
                     /locus_tag="VYO97_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1752931.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            28596..29531
                     /locus_tag="VYO97_000032"
     CDS             28596..29531
                     /locus_tag="VYO97_000032"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1752932.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            29618..30130
                     /locus_tag="VYO97_000033"
     CDS             29618..30130
                     /locus_tag="VYO97_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1752933.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            30167..30762
                     /locus_tag="VYO97_000034"
                     /pseudo
     CDS             30167..30762
                     /locus_tag="VYO97_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            30863..31045
                     /locus_tag="VYO97_000035"
     CDS             30863..31045
                     /locus_tag="VYO97_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1752934.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(31091..32536)
                     /locus_tag="VYO97_000036"
     CDS             complement(31091..32536)
                     /locus_tag="VYO97_000036"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1752935.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLREQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QQPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            32642..34264
                     /locus_tag="VYO97_000037"
     CDS             32642..34264
                     /locus_tag="VYO97_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1752936.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            34467..36425
                     /locus_tag="VYO97_000038"
     CDS             34467..36425
                     /locus_tag="VYO97_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1752937.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            36597..37142
                     /locus_tag="VYO97_000039"
     CDS             36597..37142
                     /locus_tag="VYO97_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1752938.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            37151..37690
                     /locus_tag="VYO97_000040"
     CDS             37151..37690
                     /locus_tag="VYO97_000040"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752939.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            37726..38271
                     /locus_tag="VYO97_000041"
     CDS             37726..38271
                     /locus_tag="VYO97_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1752940.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     IDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            38428..39522
                     /locus_tag="VYO97_000042"
     CDS             38428..39522
                     /locus_tag="VYO97_000042"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1752941.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            39530..40696
                     /locus_tag="VYO97_000043"
     CDS             39530..40696
                     /locus_tag="VYO97_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1752942.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            40789..43887
                     /locus_tag="VYO97_000044"
     CDS             40789..43887
                     /locus_tag="VYO97_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1752943.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNMAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            43935..44552
                     /locus_tag="VYO97_000045"
     CDS             43935..44552
                     /locus_tag="VYO97_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1752944.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            44563..45897
                     /locus_tag="VYO97_000046"
     CDS             44563..45897
                     /locus_tag="VYO97_000046"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1752945.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            45907..46371
                     /locus_tag="VYO97_000047"
     CDS             45907..46371
                     /locus_tag="VYO97_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1752946.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEMAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            46368..46862
                     /locus_tag="VYO97_000048"
     CDS             46368..46862
                     /locus_tag="VYO97_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1752947.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            46943..48598
                     /gene="mltG"
                     /locus_tag="VYO97_000049"
     CDS             46943..48598
                     /gene="mltG"
                     /locus_tag="VYO97_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1752948.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVSTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            48669..49151
                     /gene="greA"
                     /locus_tag="VYO97_000050"
     CDS             48669..49151
                     /gene="greA"
                     /locus_tag="VYO97_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1752949.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(49319..49396)
                     /locus_tag="VYO97_000051"
                     /pseudo
     CDS             complement(49319..49396)
                     /locus_tag="VYO97_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            49524..49874
                     /locus_tag="VYO97_000052"
     CDS             49524..49874
                     /locus_tag="VYO97_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1752950.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSL"
     gene            50380..51387
                     /locus_tag="VYO97_000053"
                     /pseudo
     CDS             50380..51387
                     /locus_tag="VYO97_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            51462..51662
                     /locus_tag="VYO97_000054"
     CDS             51462..51662
                     /locus_tag="VYO97_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1752951.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            51697..52413
                     /gene="atpB"
                     /locus_tag="VYO97_000055"
     CDS             51697..52413
                     /gene="atpB"
                     /locus_tag="VYO97_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1752952.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLPYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            52427..52921
                     /gene="atpF"
                     /locus_tag="VYO97_000056"
     CDS             52427..52921
                     /gene="atpF"
                     /locus_tag="VYO97_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1752953.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            52921..53457
                     /locus_tag="VYO97_000057"
     CDS             52921..53457
                     /locus_tag="VYO97_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1752954.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            53472..54977
                     /locus_tag="VYO97_000058"
     CDS             53472..54977
                     /locus_tag="VYO97_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1752955.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            54993..55871
                     /locus_tag="VYO97_000059"
     CDS             54993..55871
                     /locus_tag="VYO97_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1752956.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            55957..57363
                     /gene="atpD"
                     /locus_tag="VYO97_000060"
     CDS             55957..57363
                     /gene="atpD"
                     /locus_tag="VYO97_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1752957.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            57374..57793
                     /locus_tag="VYO97_000061"
     CDS             57374..57793
                     /locus_tag="VYO97_000061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1752958.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(57865..59171)
                     /locus_tag="VYO97_000062"
                     /pseudo
     CDS             complement(57865..59171)
                     /locus_tag="VYO97_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(59298..59531)
                     /locus_tag="VYO97_000063"
     CDS             complement(59298..59531)
                     /locus_tag="VYO97_000063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752959.1"
                     /translation="MFSQLHMREEQVLLAQDYPLETARAEGLEQGLERGKVEGREERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(59524..59640)
                     /locus_tag="VYO97_000064"
                     /pseudo
     CDS             complement(59524..59640)
                     /locus_tag="VYO97_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            complement(59855..60205)
                     /locus_tag="VYO97_000065"
     CDS             complement(59855..60205)
                     /locus_tag="VYO97_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1752960.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            60461..63094
                     /gene="polA"
                     /locus_tag="VYO97_000066"
     CDS             60461..63094
                     /gene="polA"
                     /locus_tag="VYO97_000066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1752961.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLTKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            63179..63616
                     /locus_tag="VYO97_000067"
     CDS             63179..63616
                     /locus_tag="VYO97_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1752962.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKTAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            63657..63734
                     /locus_tag="VYO97_000068"
                     /pseudo
     CDS             63657..63734
                     /locus_tag="VYO97_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(63889..64899)
                     /locus_tag="VYO97_000069"
     CDS             complement(63889..64899)
                     /locus_tag="VYO97_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1752963.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSHKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            65048..66217
                     /locus_tag="VYO97_000070"
     CDS             65048..66217
                     /locus_tag="VYO97_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1752964.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            66214..66984
                     /gene="recO"
                     /locus_tag="VYO97_000071"
     CDS             66214..66984
                     /gene="recO"
                     /locus_tag="VYO97_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1752965.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            66981..67973
                     /gene="plsX"
                     /locus_tag="VYO97_000072"
     CDS             66981..67973
                     /gene="plsX"
                     /locus_tag="VYO97_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1752966.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            67979..68212
                     /locus_tag="VYO97_000073"
     CDS             67979..68212
                     /locus_tag="VYO97_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1752967.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            68258..68432
                     /locus_tag="VYO97_000074"
                     /pseudo
     CDS             68258..68432
                     /locus_tag="VYO97_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        20260 a        12815 c        15689 g        19668 t
ORIGIN      
        1 tgaaaaaatc gctcaaagtc aggtagcttg ggcgattttg tttatcttgc ttttttttat
       61 tatcattcga tatcttatca agacttcgga caagcgagag aagaagatta tggatttgca
      121 cgagcaatca aaggccgact ctaatagacg agaagagcgt ttgatgactc acctagaaaa
      181 gaccactaca gaattaacca caatcactca cacggtcgga gacattcaaa aagaaatggt
      241 tcgcatgaac gaccgcatgg aagaaatcga aaaaggagaa taacaaatgc aacaaattac
      301 tgaaatcatt actaatggag caatcagcat cctagtcgtt ttggcagggg ttgtagttag
      361 ggcagtcaag gactacctgg ttcaaaaagg tggagaaaag accatcaaga ttgttgaaat
      421 cttggccaaa aatgcagtaa atgccgtgga gcaggtagct gctgaaactg gctacaaggg
      481 agatgaaaaa ctggcacagg ctcgcgctaa agtccgtgct gagcttacaa aatacaatat
      541 tagcatgact gacaaagact tagacacctt cgtagagtca gcagtgaagc agatgaatga
      601 cgcatggaaa ggacgatagg gaatggatat cgatagaaac agactacgta caggcttgcc
      661 acaggttggg gtgcagcctt atcgacaagt acatgctcac tcaacaggta accgcaactc
      721 aaccgtacag aatgaagcgg attatcactg gcggaaagac ccagaattag gttttttctc
      781 gcacattgtt gggaacggtt gcatcatgca ggtaggacct gttgataatg gtgcctggga
      841 cgttgggggc ggttggaatg ctgagaccta tgcagcggtt gaactgattg aaagccattc
      901 aactaaagaa gagttcatga cggactaccg cctttatatc gaactcttac gcaatctagc
      961 agatgaagca ggtttgccga aaacgcttga tacagggagt ttagctggaa ttaaaacgca
     1021 cgagtattgc acgaataacc aaccaaacaa ccactcagac catgtggatc cataccctta
     1081 cttggcaaaa tggggcatta gccgtgagca gtttaagtat gatattgaga acggcttgac
     1141 gattgaaaca ggctggcaga agaatgacac tggctactgg tacgtacatt cagacggctc
     1201 ttatccaaaa gacaagtttg agaaaatcaa tggcacttgg tactactttg acagttcagg
     1261 ctatatgctt gcagaccgct ggaggaagca cacagacggc aactggtact ggttcgacaa
     1321 ctcaggcgaa atggctacag gctggaagaa aatcgctgat aagtggtact atttcaacga
     1381 agaaggtgcc atgaagacag gctgggtcaa gtacaaggac acttggtact acttagacgc
     1441 taaagaaggt gctatggtat caaatgcttt tatccagtca gcggacggaa caggctggta
     1501 ctacctcaaa ccagacggaa cactggcaga caagccagag ttcacagtag agccagatgg
     1561 cttgattaca gttaaataga tagaaaggaa actttctaaa attgttcttt caccgcaggc
     1621 tcaggcttgc ggttttttat ttgctcaaaa ggggcaaaaa aggggcaaaa gtgtcgtaaa
     1681 tctctgtaaa atgatgtaaa aacatttatt taaaagctca aaatatagct gttttaaaag
     1741 gtattgtaag atatagtaaa acgatgtaaa ggtattttta aatgccgatg aattataaaa
     1801 ccacgaatcc tatgtgactc gtggttcttt tttataaact ggtagagtgt tttggttgta
     1861 ctttttgttc agggtcaatg tagttgatag cgttgttgac agcagttgga gcttctccga
     1921 ggcctgtcgc aatcagatca atttttccgt catagtagca gcagtcaccg atagcataga
     1981 tacctgcttg gctggattct tgtttgctgt tgacgataat cttgtgacgg ttgaggtcga
     2041 gcccccagtt tttaaggtta ccgacagaag atttgaaacc atagttgaca aagaggtggt
     2101 ctaggtcaat ggtttcagtt tcatcagatt tgacttttgt gatttcaagt ttatcaagtg
     2161 tttttccatt tccaaggagt tggccagggg cgaatggtgt tttgatggtt acagatgatt
     2221 cttgcaaggc ttgaacactg tgttccaagg cacggaaatt atctctgcgg tgaacaaggg
     2281 tagttggtgc gattttttca aaagccaaag cccaatccac agccgagtct cccccaccaa
     2341 gaatcgtcac tttcttacca gcgtattgct gaatgttaga aacgtggtag tggatatttt
     2401 catagccctc aaccccttca agttccagcg gacgtggttt gaaggcaccg ccacccatag
     2461 cgatgataac tgttttagtc aggtgacttc ctttagaagt tgtgatggca aattcttctt
     2521 cttgtttgtc aatctcaaga accgtttcat tgagatgaat aggggtatca aatccattta
     2581 gctgttcaat caagcggtta gtcaactctt ctccagtcag gtttgggaag cctggtacgt
     2641 ctaggatttc cttttcaggg tagagaatag caggttgtcc acctagctgg ggaagagagt
     2701 cgatgatttg aaccttggct tggcgtaggt gggcataaaa ggctgcaaaa agcccgacag
     2761 gaccaccacc cacaatggta atatcataga gttgagacat agtttctcct ttgttttttc
     2821 tagtcagttt attttatcat atttttgttc aatgtaaaat ggactaggat agggaaaaaa
     2881 atgcccgaaa atggtataat aggacgaaag tgttttgata gggaggatat cagggatgaa
     2941 ttttcaacaa ttatccaatc tgcaatattg gaccagtttg tttgcaagtc catggacgat
     3001 agctatcaat ctgattgata ttttgattgt tgcttatatt ttataccatt ttacaaaagc
     3061 tattgcagga accaagatta tgattttggt acgtggagtt ttggtattta ttttagctca
     3121 aatccttgca aatatgattg gtttgactac gatttcttgg ttaatcaatc aaattattac
     3181 ttatggggtt attgcggcgg ttgttatctt ctctccagag attcggactg gtttggaacg
     3241 tttgggaaga gcgacagatt tcttttccaa tgcccctatt agtgctgagg aacagatgat
     3301 tcgtgccttt gttaagtctg ttgaatacat gagtcctcgt aaaatcgggg ccttggttgc
     3361 tattcagcgt gtacgtacct tgcaggagta tatttcgaca ggaatcccct tggatgctaa
     3421 gatttctgca gaacttctca ttaacatttt tattcccaac actcccctac atgacggtgc
     3481 ggtgattatc aaagaagaac gtatcgctgt gacgtctgcc tatctgccct tgacaaaaaa
     3541 cacaggtatt tccaaggaat ttgggaccag acaccgggcg gctatcggtt tatcagaagt
     3601 ctcagatgcc ttgacttttg tcgtatcaga ggaaacggga ggaatttcga taacctataa
     3661 tggaaggttt aagcacaacc taacacttga tgaatttgaa acagaattac gtgaaatctt
     3721 acttccaaaa gaggaagtgg gtcttagttt taaagaacga tggctaggag gatggaaaca
     3781 tgaaaaaaaa tagtttatat atcatatcct cactcttttt tgcttgtgtc ttatttgtct
     3841 atgctacggc gacgaatttt caaaacagta ccagtgctag gcaggtaaaa acggaaacct
     3901 atactaatac agtaacaaat gtccctattg acatacgcta taatagtgat aagtatttta
     3961 ttagcggttt tgcttcagaa gtatcagtgg tcttgactgg tgcaaatcgc ctatcgctag
     4021 ctagtgaaat gcaagaaagt acacgtaaat tcaaggttac tgctgaccta acagatgccg
     4081 gtgttggaac gattgaagtt cctttgagca ttgaagattt acccaatggg ctgaccgctg
     4141 tggcgactcc gcaaaaaatt acagtcaaga ttggtaagaa ggctcagaag gataaggtaa
     4201 agattgtacc agagattgac cctagtcaaa ttgatagtcg ggtacaaatt gaaaatgtca
     4261 tggtgtcaga taaagaagtg tcgattacga gtgaccaaga gacattggat agaattgata
     4321 agattatcgc tgtcttgcca actagcgaac gtataacagg taattacagt ggttcagtac
     4381 ctttgcaggc aatcgaccgc aatggtgttg tcttaccggc agttatcact ccgtttgata
     4441 caataatgaa ggtgactaca aaaccagtag caccaagttc aagcacatca aattcaagta
     4501 caagcagttc atcggagaca tcttcgtcaa cgaaagcaac tagttcaaaa acgaattaaa
     4561 aatagaaaag gattttataa caatgggtaa atattttggg actgatggag tccgtggaga
     4621 agctaaccta gaactaacac cagaattagc ctttaaacta ggacgttttg gaggctatgt
     4681 tcttagtcaa catgaaacgg aagcgccgaa agtctttgta ggacgtgaca cacgtatttc
     4741 aggggaaatg ctggaatcgg ccttggtggc aggtctcctt tcagtaggga ttcacgtata
     4801 caaacttggt gtccttgcaa caccagcagt agcttacttg gttgaaactg aaggagcaag
     4861 tgccggtgtc atgatttctg ctagccacaa cccagccctt gataacggaa tcaagttctt
     4921 tggcggtgat ggcttcaaac tagatgatga aaaagaagca gaaattgaag ccttgctaga
     4981 tgctgaggaa gacactcttc ctcgtccaag tgcagaaggc ttaggaattt tggtagatta
     5041 tccagaaggc ttgcgtaagt atgaaggata ccttgtttca actggaactc ctcttgatgg
     5101 aatgaaggtt gccttggata cagctaatgg agcagcttct accagtgccc gtcaaatctt
     5161 tgcagacctt ggtgcccaat tgacggttat cggggaaaca ccagacggtc ttaacatcaa
     5221 ccttaatgtt ggttcaacac atccagaagc ccttcaagaa gtggtcaaag aaagtgggtc
     5281 agctattggt ttggcctttg atggagacag tgaccgcttg attgctgttg atgagaatgg
     5341 tgacatcgtc gatggtgaca agattatgta catcatcgga aaataccttt ctgaaaaagg
     5401 acaattggct caaaatacaa ttgtgacaac tgttatgtct aaccttggtt tccacaaggc
     5461 cttgaatcgc gaaggtatta acaaggcagt tactgcagtt ggtgaccgct acgttgttga
     5521 agaaatgaga aaatcaggct acaaccttgg tggtgaacag tctggtcacg ttatcttgat
     5581 ggattacaat accacaggtg atggtcaatt atcagcagtt caattgacta aaattatgaa
     5641 gaaaactggt aaaagcttat cagagttggc ggcagaagta acgatttatc cacaaaaatt
     5701 agttaatatc cgagtggaaa acgtcatgaa ggaaaaggcc atggaagtgc cagctatcaa
     5761 ggccatcatc gagaagatgg aagaagaaat ggcggggaac ggccgtatcc ttgttcgtcc
     5821 aagtggaaca gagcccctct tgcgtgttat ggcagaagcg cctacaacag aagaagtgga
     5881 ctactatgtt gatactatca cagatgtagt tcgtgctgaa attgggattg actaaatcct
     5941 agaaaactag atgaaaataa aaaattatgg tacaatagct tataatattg tagccgatgg
     6001 agaaagacat gaatcttaaa cgtgaacaag aatttgttag tcagtatcat tttgatgcgc
     6061 gtaattttga atgggaaaat gaaaatggag ctcctgaaac caaggtggat gtgaatttcc
     6121 aattgctcca acatgaccaa gaaaatcaag tgacttcctt gattgtcatc ttgagcttta
     6181 tgattgtatt tgataagttt gtcattagtg gaacaatctc acaagtaaac catatcgatg
     6241 gccgtattgt caatgaacca agtgaattga accaagaaga ggtggaaacc ttggctcgtc
     6301 catgtttgaa catgctcaat cgtttgactt acgaagtgac tgaaattgca ttagacttac
     6361 caggaattaa tttggagttc taatatgaaa ttagctgttt tcacagattc ctctgcttat
     6421 ctcagtgaag agaccttgca aagagaagac ttgtttgtct tggatattcc tgtcaatatt
     6481 gatggtgagg aatatgtcga aggcatcaat ctgtctgctg aggaatttta ccaaaaaatg
     6541 gctcaggctt ctgaattgcc taagaccagt caaccaagta ttgccaagtt agatgagatc
     6601 ttaacttcgc tcaaagaaca aggctataca catgccttgg ggcttttcct atcttctgga
     6661 atttcaggtt tttaccaaaa tatccagtat atggtcgatg actatgaggg cttaaccatt
     6721 gctttcccag acactttgat tacaagtgct cccctaggca tcatggttga aagcgtcttt
     6781 aattggcgtg atcagggcga tgattttgcc agcattcagg ataagctagc cattcaaatc
     6841 agccgtacgt cagcctttat catggtagat gacttggatc atttggtaaa aggtggacgc
     6901 ctttcaaatg gggctgccat tttgggcaat ttgcttagca ttaagccaat cctttatttt
     6961 aacgatcaag gtgtgattga agtttacgaa aaagttcgta ctgaaaagaa ggccaccaag
     7021 cgcttaattg aaattatcaa ggaaacaacg gcttcaggtc aataccgggt cattgtcatt
     7081 catgggaatg ctcctgaaaa ggctgaagaa ttgcgtcagc acttgcttga ttttggcttg
     7141 ggttcggatg tttcacttgc tacatttggt agtgtcattg gaacgcacct aggagcagga
     7201 agtattgctc tgggttatat tccagtgatt tagttggcgc ttctaggcta gttaagatgc
     7261 cttgtatctt agaaattgaa cacggactac ctgcctcttg aaaaaagatg cctgtcttgc
     7321 ctttcgtgga aagtcagtgc cattccctat ttttcagagg cagctaaggt cctttgtatc
     7381 ttgataattg aacacgcctg gaaccctgtg tgaaaaagat agttcttcca agaagtagac
     7441 acttcttgat tggaattcct attttcactt tgtgttctta cggactttgt atcttagaca
     7501 ggagtagaga tgagtattcg agtaattatt gccggtttta agggaaagat gggccaggct
     7561 gcttgtcaga tggtattgac tgatccagac ttggacttgg tagcagtttt ggatcctttt
     7621 gagtctgagt cagaatggca gggtattcct gttttcaagg ataaggctga tttagcttgt
     7681 tttgaagcgg atgtctgggt agattttact actccagctg ttgcctacga aaatacacgt
     7741 tttgctcttg aaaatggctt tgctccagta gttggaacga ctggtttcac gagtgaagaa
     7801 attgcagagc taaaagaatt ttctcgtacc caagacttgg gtggcctgat tgcccctaac
     7861 tttgccttgg gtgctgtctt actcatgcaa tttgcgacgc aggctgccaa atatttccca
     7921 aatgtggaga ttattgagct ccatcatgac aagaaaaagg atgctccgag tggaacagcc
     7981 attaaaacag ctgagttgat ggcagagatt cgagagtcaa ttcagcaagg tgcagcagat
     8041 gaggaagagc tgattactgg tgctcgtggt gctgactttg atggtatgcg catccactca
     8101 gttcgtttgc caggtttggt agcccatcag gaagtcatct ttggcaatca aggagaagga
     8161 ttgaccctcc gtcatgactc ctatgatcgc atctccttca tgacaggagt caatttggga
     8221 attaaagaag ttgtcaagcg tcatgagctt gtctatggat tagaacactt attatgagat
     8281 taatgcaaat gccttctgaa tttcagaagg ctttaccagt attagaaaaa attaaagaag
     8341 caggctttga ggcttatttt gttgggggct ctgttcgaga tgcccttctc catagcccta
     8401 tccacgatgt ggatattgcg acgtcttctt atccagaaga gaccaagcag atttttccgc
     8461 gaacagccga tatcggaatc gagcatggaa ccgtcttggt cttagatggg gatgaggagt
     8521 atgaggtaac aacctttcgg acagaggatg tctatgtgga ctatcgcaga cccagtgcgg
     8581 tttcctttgt gcgctcgcta gaagaagacc tcaaacgccg tgatttcaca gtcaacgcct
     8641 ttgccttgga tgagacagga gaaatcgttg acttgttcca tggtttagaa gatttggaaa
     8701 agcaagtctt gcgagcagtt ggagtggcta gtgagcgttt caacgaagat gctttacgga
     8761 ttatgcgtgg tttccgtttt caggctagtc ttggttttgc acttgagcca gaaacattta
     8821 aagctatgaa gaccttgacg ccgcttttgg agaaaatttc tgtagagcgt accttcgtcg
     8881 agtttgataa actcttgctg gctccatttt ggagaagggg cttggcttcc atgattgaga
     8941 gtcaagctta tgactatctc cctgatatgg catctagcca ggacaagctc aacagactgt
     9001 ttgatttgga gactgatttt acttttgaat cctctgaaca agcctgggcg gctttactat
     9061 gggctttgga gattgaaaat gcgcagtcat ttttgaaatc ttggaagacc tcacgccagt
     9121 ttgccaagca agttcaggat ttgctgatta ttttggctct gcgtgaaaat ggagaattga
     9181 gcaagcgaga ttgttatcgc tttgacatag atttgctttt acaggctgaa aatcttcgtc
     9241 aagctcaagg aaaagaagtc aacccacaag ccatcacaga aaaataccaa agcttgacta
     9301 tccatgacaa gaaagatatt cagattaatg gcggtatttt gatcaaggaa tatggctatc
     9361 agccaggccc agacttggga gagattttaa cagagattga gtttgccatt gtcgatggag
     9421 aattggagaa taatcgtgaa gccatccatg cttacctgag ggagaaaaaa tgagtgattt
     9481 tatcgttgaa aaactaagta aatccgttgg tgacaagacc gtttttaggg atatttcctt
     9541 tattatccat gacttagaca gaattggttt aatcggtgtc aatgggactg gcaagaccac
     9601 ccttttggac gtcctttctg gtgtttctgg atttgatggg gatgtcagtc ctttttcagc
     9661 taaaaatgat taccagattg gttacttgac tcaggatcct gattttgatg atagaaagac
     9721 agttttggat acggttctat ctagtgaact caaggaaatc cagctcattc gtgagtatga
     9781 attgattatg ctcgactata gtgaggacaa gcaggcgcgt ttggaacgtg tcatggcaga
     9841 gatggactct ctccaagctt gggaaatcga aagtcaggtc aagaccgttc ttagcaaatt
     9901 gggcattcaa gacttatcta ctcctgttgg ggaattgtca ggtggtctga gaagacgggt
     9961 acagttggca caagtcttac ttggcaacca cgatctcttg cttttggatg agccgaccaa
    10021 ccatctggat attgcgatta ttgagtggct gaccctcttt ttgaaaaatt ctaagaagac
    10081 cgtccttttt atcactcacg atcgttattt cttagacgct ttgtcaacac ggattttcga
    10141 gttggatcgt gcaggcttga ccgagtacca gggaaattac caggactatg ttcgcctaaa
    10201 ggcggaacag gatgagcgcg acgcggctct tcttcacaaa aaagaacaac tctacaaaca
    10261 agaattggcc tggatgcgca gacaaccgca ggcgcgtgcg accaagcagc aggcccgtat
    10321 caatcgattc catgatctga aaaaggaagt ttcaggcagt agtgctgaga cagacttgac
    10381 catgaacttt gaaaccagtc ggattgggaa gaaagtcatc gagtttcagg ctgtttcctt
    10441 tgcctatgaa aacaagccta ttttgcaaga ttttaatctc ttggtgcagg ccaaagaccg
    10501 tatcggaatc gttggggaca atggtgttgg aaaatcaacc ctgctcaact tgattgcagg
    10561 aagtcttgag ccaactaaag gacaagttgt gattggggaa actgttcgca tcgcctattt
    10621 ctctcagcaa attgagggtt tggatgaaag caagagagtt atcaattacc tgcaggaagt
    10681 ggcagaagag gtcaagacca gtggcggttc tacgacttcg attgcagagt tgctggagca
    10741 gttccttttc ccacgttcga cgcatggaac cttgattgag aaattgtcag ggggtgagaa
    10801 aaaacgtctt tatctcctca aactgctttt ggaaaaacca aatgttcttc ttttagacga
    10861 gccaaccaat gacctagata ttgcaacttt gacagtcttg gagaatttct tgcaaggttt
    10921 tgcaggtccc gttttaacag tcagtcacga ccgctatttc ttggataagg tagcgaccaa
    10981 gattctcgct tttgaggatg gcaagattcg tcctttcttt ggtcattaca ccgactatct
    11041 tgatgaaaaa gcttttgaaa cagatatggc caatcaagtg caaaaggccg aaaaggaaaa
    11101 agtggtcaag gttcgagaag acaagaaacg catgacctac caagaaaagc aggagtgggc
    11161 aagtattgaa ggtgatattg aaaccttgga aaaacgtatc gctgctattg aagaggaaat
    11221 gcaggctaac ggctctgact ttggtaagct ggctactctc caaaaagaat tggatgagaa
    11281 aaatgaagca ctccttgaaa aatacgaacg ctatgagtat ctcagtgaat ttgatagtta
    11341 agactattcg aaaatcaaag tgcaaactat gtcggcttca cattgccgac tcaagtacag
    11401 cctgtgaccg gcttcctaat ttgcactttg atttttattg agtataaaag aatagaaaaa
    11461 tcccgtctca tgaaaggatt tctctattct tttttggttt cttgatgaaa gatattttgc
    11521 caggtttcgt ggcgacgcca gatactggta tggatgatac catctaactc atagcagata
    11581 agtttggttt tttgactgat ggaagtaatt tgaatgtcct tgatagcaga atttaactct
    11641 tttttggtct tataagcctc tttatccatc tgctctccat cttggcgaat atagacaaaa
    11701 tcatcagtca agagttcttc tagttggttt ccttggtcga tcaattgttc acgcataagc
    11761 aattttttat agacattgtc taaaatttca tcctcaggga taggtgctgg ttcgatatgg
    11821 acatcggtat caaagacgcc aaaacgctcc tccagcatag actcgacctg atccgcgatt
    11881 tcatggcttt caaaaacaga caagtcagga ttcatctcta gtgtaatatc caggtagatg
    11941 ttgctaccgt aggtgcgacc tctttgcgat ttgaccttgc tgattttggg aatttccatg
    12001 atagcctttt ggtagtcctc gagcaggcgg tcgtcaaagc catctgaaag actaaaggaa
    12061 gactcgatga agatatcata ggcagtcttc aagataaaga aagtgatgat gatagcaacc
    12121 agtttatcca caatcggata attgaaacta ctagctagga tggcaatggc ggttccaagt
    12181 gaggtaacag cgtcagaaag attgtcctta gcagctgcct tcagcgcctt ggagttggat
    12241 ttcttactga ggcgagtatt gtagagatag accacaaaca taatcgctgc agaaatgatt
    12301 cctagagttg caccaagagg atcaatgacc gtttcttccc gactgagaat cttttgaatg
    12361 gtatctctta gaacatcgaa accgacatag aacatgatga tagaagtgat caagcttgcc
    12421 aaatcttcaa tcttccaatg accaaaacgg tggtcacggt ctgcaggctg gcgcgccatc
    12481 cgaatcccga ttaagagggc cacatttcca atgatgtccg atacgttatt aaaaccatcg
    12541 gccaccaaac tggatgaatg aaggagatga ccagctgcta atttggctgc agacaagatc
    12601 aaataggtcg aaatactgat aatggctcca cgctcagcta acttgagatt tgagatagat
    12661 tgcttcattc aacttccttt ctagtcacat agcattctac cattatactg gttttcaagg
    12721 aaaaattcaa ataaggcagt ttttactctt tgaaaactcc ccaaaatttg gtataatggt
    12781 actactcaag ggagtagctg gcagaaacct gtgatagtgt cgtcattccg aattttatgc
    12841 tgaaaagtat gctttccggc cctatcttaa acagcgagac ttgttatgat taacaggtct
    12901 ctttttgttt gtcgtgaaaa gatatgacag aaagagagtc tgttttgcac acaatgtcaa
    12961 ggaggagaca catgtcaaaa gaacaaaaac gccaagcgtt ttatactcag agccctgaag
    13021 aggtcttgca ggctgtggat gcgaccgagc aaggtttgtc atcaagtgag gcggaaaagc
    13081 gccttgccga atttgggcac aatgaactcg aagaaggcga gaaacgatca atcttggtca
    13141 aattcatcga gcaatttaag gatttgatga ttatcatcct agttgcggca gcaatcttgt
    13201 cagtcgtgac ttctggtggg gaagatatcg cagatgccat tatcatccta gctgtggtta
    13261 tcatcaacgc tgcctttggt gtttaccaag aaggaaaagc tgaagaagct attgaagccc
    13321 tcaaatccat gtctagtcca gttgcccgcg ttcttcgtga tggacacatg gcagagattg
    13381 actctaaaga attggtacca ggcgatatcg ttgcccttga agcaggtgac gtggtaccag
    13441 cggacctacg tttgctagaa gccaactctc ttaaaattga agaagcagcc ttgacgggtg
    13501 aatctgtacc agtcgaaaaa gacttgtcag tcgagcttgc gacagatgct ggtattggtg
    13561 accgtgtcaa catggccttc caaaactcaa acgtaaccta tggtcgtggg atgggtgttg
    13621 ttgtcaatac aggtatgtac actgaagttg gtcatattgc tggtatgctt caagatgcgg
    13681 atgagactga tacaccactc aaacaaaact tgaacaacct ttctaaggtc ttgacctatg
    13741 ctatcttggt cattgccctt gttacttttg tagtgggtgt cttcattcaa gggaaaaatc
    13801 cacttggtga gttgttgact tctgttgccc ttgctgttgc agccattcca gaaggacttc
    13861 ctgctatcgt taccatcgtt ctttctcttg gtactcaagt tttggccaaa cgacattcca
    13921 tcgttcgtaa gttgccagca gttgaaacac ttggttcaac tgaaatcatc gcttctgata
    13981 agactggtac gctgactatg aacaagatga cagtcgaaaa agtcttctac gatgcggttc
    14041 tacatgactc agctgatgat attgaactag gtcttgaaat gccactactt cgttcagttg
    14101 tcttggccaa tgatacgaaa atcgatgtgg aaggtaactt gattggtgac ccaaccgaaa
    14161 cagcctttat ccaatatgcc ttggacaagg gctatgatgt caaaggtttc ttagagaaat
    14221 atcctcgtgt agctgaattg ccatttgact ctgaccgtaa gctcatgtca acagttcacc
    14281 cattgccaga tggtcgtttc cttgtagcag tcaagggtgc gccagaccaa ctcttaaaac
    14341 gttgtcttct tcgtgataag gctggggata ttgctccgat tgatgagaag gttacaaatc
    14401 tcattcatac aaacaattct gaaatggctc atcaagcctt gcgtgtcctt gcaggtgctt
    14461 ataagattat cgatagtatt ccagaaaatc tcacttctga agagcttgaa aatgatttaa
    14521 tttttactgg tttgattggg atgattgatc ctgaacgtcc tgaagctgct gaggctgttc
    14581 gtgtggctaa ggaagctgga attcgtccaa ttatgattac aggtgaccac caagacaccg
    14641 cagaagctat tgccaaacgt ttgggaatca ttgacgcaaa cgatacagaa gaccacgttt
    14701 taactggtgc tgagctcaac gaattatctg atgaagactt tgaaaaagtt gttggtcaat
    14761 actctgttta tgcccgtgtg tctccagagc acaaggttcg tatcgtcaag gcttggcaaa
    14821 aacaaggtaa ggtcgttgcc atgacaggtg acggtgtcaa tgatgcccca gctctgaaaa
    14881 cagcagacat cggtatcggt atgggaatca ctggtacaga ggtttctaag ggggcttctg
    14941 atatgattct tgcagatgat aactttgcga ctattatcgt cgcagtggaa gaaggacgta
    15001 aggtcttctc aaatattcaa aagactattc agtacctact ttctgccaat actgctgaag
    15061 tattaaccat cttcctatca accttgtttg gttgggacgt cttgcagcca gttcatcttt
    15121 tgtggattaa cttggtaacg gataccttcc cagcaattgc actaggtgtt gaacctgcgg
    15181 aacctggtgt catgaatcat aaaccacgtg gacgcaaggc aagcttcttc tcaggtggtg
    15241 ttttgagttc tatcatttat caaggtgtac tccaagcagc tcttgttatg agtgtttatg
    15301 gccttgcgat tgcttaccca gttcatgtgg gtgacaatca tgctattcat gcagatgccc
    15361 taacgatggc ctttgcaacc cttggtttga ttcagctctt ccatgcctac aatgtcaagt
    15421 ctgtctacca atccatcttg acagttggcc cattcaagtc taagaccttt aactggtcca
    15481 tcttggtatc ctttatcctt ctcatggcaa caatcgttgt agaaccgctt gaaggaatct
    15541 tccacgtaac caaactagac ttgtcacaat ggggaattgt tatggctgga agcttctcaa
    15601 tgattatcat cgtcgaaatc gttaagttta ttcaacgcaa actcggtttt gacaagaatg
    15661 cgatttaagg gaggtttcgg atgtcagcat atcaattacc gaccgtatgg caggatgaag
    15721 ctagtaatca aggagctttt acggggctaa acagaccaac agcaggtgcc cgtttcgaac
    15781 aaaacttgcc aaaaggagaa caagcttttc agctttattc actgggaaca ccaaatggtg
    15841 tgaaggttac tatcttattg gaagaattac tagaagctgg ttttaaggaa gcggcttacg
    15901 acttgtataa gattgctatc atggatgggg atcaattcgg ctcagacttt gtgaagctca
    15961 atccaaattc caagattcca gccttattgg accagtcagg tactgaaaac gtaagagtct
    16021 ttgagtctgc tcatattctt ctttaccttg ctgagaaatt tggagccttt ttaccaagta
    16081 atcctgtgga aaaggtagaa gttttgaatt ggctattctg gcaagcaggt gcagcacctt
    16141 ttctaggtgg gggatttgga catttcttca attatgctcc tgaaaaattg gaatatccta
    16201 ttaaccgttt tacgatggaa gtgaaacgcc agttggattt attggataag gaattggctc
    16261 agaaacctta tattgcaggc aatgactata cgattgcaga tattgctatc tggtcttggt
    16321 atggacagtt agttcaagga aatctttacc aaggttctgc aaaattcttg gatgcctcaa
    16381 gttatcaaaa tctagtaaaa tgggcagaaa aaattgccaa tcgtccagct gttaagcgtg
    16441 gcttggaagt aacttataca gaaattaaat agcaaaaaag tcatcttcgg atggcttttt
    16501 gctacagttg agggaaaggg tttgcagtta tcggcttttg tgctataatt gctataatat
    16561 agtaaaaagc aagaggagtg tgaggaagtg gaaaagaaaa ttgtgaagga tatcttattt
    16621 ttatctcaag tgtctcagcc ggcaagtcag gaggaccttt atcttgccag agatttgcag
    16681 gatacactct tagcaaatcg tgatacctgt gttggtctag ctgccaatat gattggggtg
    16741 cagaagcgcg tgattatctt taatcttggc ttggttcccg tggtcatgtt taacccagtg
    16801 cttctgtcct ttgaaggatc ctatgaggca gaagaaggct gtttgtcctt ggtaggtgtg
    16861 agatcaacta agcgttatga aaccataagg gttgcctatc gtgacagcaa gtggcaggaa
    16921 cagaccatta ccttgacagg cttcccagct cagatttgcc agcatgagct ggatcacttg
    16981 gaaggacgaa tcatttagga ggaaagcaaa tgaaacgaat agtctttgaa cttattttta
    17041 tcgcaacgac ctggtatatc tttttaccgc cccttaacct gaccagctgg gaatttctct
    17101 tcttcctctg tgggcatttg ttagttgtgg caatcttatt tggctttggc aaggggataa
    17161 accttgtcaa aacggttcat gtgcgccacg gtaaggcgga agctgcctta aatcttgagg
    17221 gtttcaaaat caatcggtta gggaaaattc tgttagcttc gattggagga attcttctct
    17281 tggcagcttt ggtttccttg gtaacttcca gcatgtttca ggctaaaaat tatgccaatg
    17341 tagtcacggt tacggaaaaa gactttactg aatttcctaa gagtgacacc agtaaggttc
    17401 ctatcctaga tagaagtact gctgaaaaaa ttggagaccg ctacttgggt tccctaaccg
    17461 ataaggtgtc gcaatacgta gcggcagata cctataccca attgacaatt gatgggaaac
    17521 cttatcgggt cacaccacta gaatatgcag accctatcaa atggtttaac aatcaagcca
    17581 agggaatcgg tgagtatatc aaggtggaca tggtaactgg aaatgcggat ttggtggact
    17641 tgaagacacc aatcaagtat tcagactcgg agtattttaa ccgtgatgtc aaacgtcacc
    17701 tgcgcttgaa gtacccgacc aaaatcttta aaactccatc ttttgaggtg gacgatgagg
    17761 gcaatccttt ctatgtagca acggtttacc aaaagcaatt tggacttgct gttcctcgtc
    17821 ctgcttcagt cattatcttg gatgctacaa atggagaaac caaggaatac agcttatcag
    17881 atgttccaga atgggtggac aggatctatc cagcagagga aaccattgag caaatcaact
    17941 acaacggcaa gtacaaggac ggtttcttga atgccatgat ttccaagaaa aacgtgaccc
    18001 agactaccaa gggctataat tacttgtcta tcggtaatga catctatctc tacacaggtg
    18061 tgacgtcggc taatgcggat gagagtaatc ttggtttcat ccttgaaaat atgcgaacag
    18121 gagaaatcac taagtatagc ttggcttctg cgacagaaga atcagcccgt gaatcagcag
    18181 aaggtgctgt tcaggagaaa tcctacaaag caaccttccc aatcctcatc aacctcaatg
    18241 acaagcctct ctacatcatg ggcttgaagg acaatgctgg cttggtcaaa gagtacgccc
    18301 tggtagacgc agtcgagtac caaaatgtta tcgttgctac tacagtggaa gagatgctca
    18361 gcaagtatgc caataaaaac gaccttgaaa ttgacaatgc aacgacagaa agcatcaatg
    18421 gagtagtagc agacctcaaa tcagctgtta tcaagggaga cactgtctac ttctttaaag
    18481 ttgatggcaa gatatacaag gtcaaggctt cagtatccga tgaccttcct taccttgaaa
    18541 atggtaaaac cttcgaaggt caagtaggaa aagacaatta tctcaagacc tttaagctac
    18601 ggtaaaaata ggtttttttc agaaagtata tgttataata aggtaaatta agcctaatgg
    18661 aggacaaaga aatgggtttt tatttgatgg ttgcttcaat gctactcgga ctgctcgctt
    18721 tgaagatagg tttttctcag ttcaaggaga aaaaagataa attcttatct atcttaacaa
    18781 gtttagctgg cacagccctt gttctcgtgg ctgtctggtt aggatggccc aaataaatag
    18841 aaaaacctcg gtcaaaccga ggtttttcaa atgaatttct tggttgtggc taaaaaatat
    18901 gctacactat caatatgaaa attttaatcc caacagcaaa agaaatgaac acagacttgc
    18961 caagtatcga ggcaatttct ttaaaaccag aaagtcaggc cgtgcttgat gccttggctc
    19021 tctattctgc cagtcaattg gagagtttct acaaggtatc agctgagaaa gcggcggaag
    19081 aatttcaaaa tatccaagct ttgaaaaggc aaactactca acactatcca gccttgaaac
    19141 tttttgatgg gcttatgtac cgcaacatta agagagataa gctgaccgag gcggaacaag
    19201 attatcttga aaatcatgtc ttcattacct cggctttgta cggtgttgtt ccagccttgt
    19261 cacccatggc tcctcaccgt ttggattttt tgatgaaatt aaaagttgct ggtaagactt
    19321 tgaagagcca ttggaaggca gtctatgatg aagctctgaa gaaggaagaa gtgattttct
    19381 ctctcttgtc atcagagttt gagactgtat tttctaagga aatcagagca aagatggtga
    19441 ccttcaaatt catggaggat agaggcggtc agctgaagat tcactcaact atctccaaga
    19501 aagcgcgcgg ggcctttcta acagctttaa tagaaaatca agtacaaact gtgggggaag
    19561 cacgtcgctt gaactttgct ggatttgttt accgagaaga tttgtcacaa ccacagggat
    19621 tggtttttgt aaaggaagta tagaaatcag atattttgta tttggcaata tgtatcatca
    19681 gctattttag tttataaatg agaaaaaagc gaacaagcta gtcttctgag cgtcagaaaa
    19741 gtagtcttgt tcgcttttat tatgttatac tcttcgaaaa tctcttcaag ccatgtcagt
    19801 gtcgccttac cgtatatatg ttactgactt cgtcagttct atctacaacc tcaaagcagt
    19861 gctttgagta gcttgcggct agcttcctag tttgcttttt gatttttatt gagtgttagt
    19921 tacttgatag cttcgatcaa gtcttgagct tgtttttcaa gtgagtttag gactgtttct
    19981 tcaagaacca attttccgtc tgcccaggca gagtcattaa cacgtgcagc agtgaaatca
    20041 ccaacgcctt gtgtacggat aaatggcaag aggtctttgt agatagcgaa gagttgatcg
    20101 tgacctgcat tggctacaga tgatactgtg acgaacttgt cttgaagggc agaagcgccg
    20161 cgtgtatcag acaagtcaag ggcacgagat agccagtcaa gcaagttttt cactgttcca
    20221 gggatagaga agttgtagac tggagagaag atccagatgg catccgcagc aaggacagct
    20281 tcacgagctg ccgctacagc tggatgagtt ggaacttcca aatcttggct gaagagagga
    20341 agggctgaat aatcaaggta gctaacttct gctttaccag caagtgcttt ctcagcttcg
    20401 agggccattt ggtggttgaa agaaccttgg cgtagtgatc cgacgataaa taatactttt
    20461 ttagacatga tgtgtctcct ttttcttaaa tttacgagaa ttttctcgtt gttataaaat
    20521 atattacaac aaagcaaaac aacttgcaag aaatttgctc agagtttatt tgaaaattat
    20581 aaaattgctg aaactctagt cacttgtgac tgaaaaagta agaactgttt cactttcaaa
    20641 atcggttttg tttgaaggga gaaatagaat tggttgctca aagcacagct ttgaggttgc
    20701 agataaagct gacgtggttt gaagagattt tcgaagagta ttcttatatt cacttgtaat
    20761 aaagcttaat agatggtaaa atagacgagt gaaaaaaaga caaaacaaag caaaaaataa
    20821 tctactgtgg cagtatggtc tagggatgac gattttgttt gtggttatca gtgcttcctt
    20881 tctgtatatg gtttctctta gcatgaaacc ctatcaaaca gctaaaagtg aaggagaaaa
    20941 attagctcag cagtatgcag gattagagca ggccgatcag gttgatttat acaatggctt
    21001 ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa gaagcacttg ctgttctgat
    21061 tggaaaagat gatcataaga tttacgttta tcagctaaat cagggtgttt cacaagaaaa
    21121 agcagaaacg gtttctaagg aaaagggagc tggcgaaatt gacaagatta tctttggtcg
    21181 ttatcaagat aagccaatct gggaagtcaa gtcaggatct gatttttatc tagtagattt
    21241 tgaaacagga gcattggtca acaaggaggg cctatgaaat tatccaaccg tgttttagaa
    21301 atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca aagcactgaa ggctgagggc
    21361 agagatattt tgtctctaac cttgggtgag ccagatttca ctactcccaa aaatatccaa
    21421 gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt tttatacagt aacctcaggt
    21481 ctgccagagc ttaaggcggt ggtcaatagc tactttgagc gcttttacgg ctattctgta
    21541 gcgccaaatc aagtgacagt cgctgcggga gccaaatatt ctctctatac cttctttatg
    21601 gctgtggtca atccaggtga tgaagtcatc atcccaaccc catactgggt tagctatgga
    21661 gatcaggtca agatggcaga aggcgttccc gtctttgttt ctgctaagga agacaatcac
    21721 tttaaggtga ccgtagagca gttagaagca gctcgcactg acaagaccaa ggttttggtg
    21781 ctgaattcgc catctaatcc cacaggtatg atttacaccc gtgaggaact cttggcaatt
    21841 ggaaactggg ctgtagaaaa tgatattctc atcctagcag atgatatcta tggccgcttg
    21901 gtttataatg gtcatgagtt cacaccgatt tctagcctat cggaagcgat tcgcaagcaa
    21961 acagtggtca tcaatggtgt gtctaaaacc tatgccatga ctggttggcg gattggttat
    22021 gccgttggag aagcagacat tatcgctgcc atgtccaaga ttgcaggtca aacaacttcg
    22081 aatccgtcag cagtagccca atatgcagca gttgaggctc tatcaggtga gcaagatact
    22141 gtagaaagca tgcgtcaggc ctttgagaaa cgtcttaata ccatctatcc cctccttgca
    22201 gaggtgccag gatttgaagt ggtcaaacca caaggggctt tctatctatt cccaaatgtc
    22261 aaaaaggcca tggagatgaa aggctacacg gatgtgacag actttacaac tgctatatta
    22321 gaagaagccg aagtggcctt ggtaacagga gctggatttg gagcaccaga aaatgtccgc
    22381 ctcagctatg cgacagacct agacacgctt aaagaagcag tcgaacgctt gaaagcattt
    22441 atgggtagtg agaatgattg atcattttga gattaaggta aaggatttac aaatttcaga
    22501 aggattttat aggagttttc tcgctccttt ggactataaa ttggctttta agactagttc
    22561 tctaattagt tttctatccc cgaacagccc tcatcctggt ggtgattttt ggctgactca
    22621 gggaacacaa gatcctgttc attttgcttt tttagcagaa aataaggaag aagttcaggc
    22681 ttgttatgag gctggcatag aggcaggtgg gcgagacaat ggggttcctg gttatcgaag
    22741 tgagcatccg atttactatg ctgcttttat gattgacctg gatgggaaca atatagaggt
    22801 ggtttgccat aaagaataaa aaagaaaagg aaaaataatg acaaaacgtg taacgattat
    22861 tgacgtaaaa gactatgttg gtcaggaagt gacgattggc gcttgggttg ccaacaaatc
    22921 aggaaaagga aaaatcgctt tcttacaatt gcgtgatgga acagccttct ttcaaggtgt
    22981 ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa gtgggacttg agaagtttga
    23041 tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg acaggtattg tcaaagagga
    23101 cgaacgttct aaatttggct atgagttgga catcacagac atcgaagtga tcggtgaatc
    23161 tcaagactac ccaatcacac caaaagaaca cggaacagac tttttgatgg ataaccgtca
    23221 cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa atccgtaacg ctattatcta
    23281 tgcaacttat gagttctttg acaagaacgg ttttatgaag tttgacagcc caattctttc
    23341 aggaaatgcg gcagaagatt ctacagaact ctttgaaact gactacttcg gaacgccagc
    23401 ctacttgagc caatcaggtc agctttacct agaagcaggg gctatggctc ttggtcgtgt
    23461 ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa acacgccgtc acttgactga
    23521 gttctggatg atggatgctg agtactcata cttgacacat gatgagtcgc ttgacttgca
    23581 agaagcttat gtgaaagctc ttctacaagg tgttcttgac cgcgcgcctc aagccttgga
    23641 aaccttggaa cgtgatacag aactcttgaa acgctacatt gcagagccat tcaaacgtat
    23701 cacttacgat caagccattg acctcttgca agagcatgaa aatgatgaag atgctgacta
    23761 cgagcatctt gagcatggtg atgactttgg gtcaccacac gaaacttgga tttcaaacca
    23821 ctttggtgtg ccaacatttg tcatgaacta tccagcagcc atcaaggcct tctacatgaa
    23881 accagttcct ggaaatccag agcgcgtgct ttgtgcagac ttgcttgctc cagaaggcta
    23941 tggagaaatt atcggtgggt ctatgcgtga ggaagattac gatgcccttg tcgctaagat
    24001 ggatgaactt ggcatggatc gtacagaata tgaattctac cttgaccttc gtaaatacgg
    24061 tacagttcca cacggaggat ttggtatcgg tatcgaacgt atggtaacct tcgcagcagg
    24121 aacaaaacat atccgtgaag ctattccatt cccacgtatg ttgcaccgta tcaaaccata
    24181 agaacaggaa aacgcccatg tggcgttttt tcgaattcta gtgtttttac ttgcgtaaaa
    24241 aaattttttc tagtataata gtttattgtg agcgaacctc acttacccct tgcaaagtct
    24301 tggggtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatc
    24361 attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt tgactctatt
    24421 ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt gggaaaaacg tcgtcttgca
    24481 tacgaaatcc aagatttccg tgaaggactt taccacatcg ttaacgttga agcaaatgac
    24541 gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca acgctgacat tcttcgtcac
    24601 atgatcgtca aaattgacgc gtaagaaggt aaactatgat taacaatgtt gtacttgtag
    24661 ggcgtatgac acgtgacgct gagttgcgtt ataccccatc aaatgtagca gttgcgactt
    24721 ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg tgaacgtgag gctgatttta
    24781 tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc taactgggct aaaaaaggct
    24841 cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta cgataaccag caaggacaac
    24901 gtgtctacgt gacagaggtc gtggctgaga atttccaaat gttggaaagc cgtagtgtgc
    24961 gtgagggcca cacaggtgga gcttactctg caccaactgc aaactattca gcacctacaa
    25021 attcagtacc agacttttca cgtaatgaaa atccatttgg agcaacaaac ccattggata
    25081 tttcagatga tgatttacca ttctaatgga caactaaact tataaaggag aaaaaacatg
    25141 gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag ttgattacat cgcagcaaac
    25201 aaaattgaat atgttgatta caaagatact gagcttctta gccgtttcgt ttcagaacgt
    25261 gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta aaaaccaacg taaagtaaca
    25321 acagctatca aacgcgctcg cgtaatggct ttgatgcctt tcgtaaacga agattaaaga
    25381 aaagacccgg ttgggtctct ttttcatgag cttgaaaaca agaaagcgaa ttaagacccc
    25441 aacgggtctt ttttcacaag ctttgaaatg agagagcgag ttaggctcag tggctccctt
    25501 tttacgagtt tgaaaacaag agaacacatt aaaagcacga tggagctttt tcataaatat
    25561 gtcttttttc tcatccaaga aattaaaata aaaatgataa actttttgaa aataggggat
    25621 ttgcctactt tatgatataa tggaaagagt tagattgaaa gaggtagtga gatggacgcg
    25681 aaattaagat acaaggcaaa gaagatcaag attgtctttt ttgatattga tgatacattg
    25741 cggaattcaa agacagggtt tattccaact acaattccca ctgtttttaa acagttgcgt
    25801 gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca tttttggtgt tgttccagag
    25861 attcgtgatc tcaagcctga cttttttgta actttgaatg gggcttatat cgaagataaa
    25921 aaaggtcagg tcatttatca gcatcagatt gaaaagtcat atgttgagga gtatatctct
    25981 tgggctaagc aagaaggaat tgagtatggc ttggttggga gtcatgatgc caagttgtcg
    26041 actcgcaccg atatgatgag tgaagctatc aatccaattt atcccgactt agatgtagat
    26101 cccgatttcc atgaaaaaga agatatctat cagatgtgga cttttgaaga taagggagat
    26161 gacttgcact tgcctgacag tctctcagac aaacttcgca tggttcgttg gcatcaacat
    26221 tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg gggtggaaaa ggttgtggaa
    26281 caccttggct tgaaaccaga gaaggtcatg gtttttggag atggtctcaa cgacttggaa
    26341 ctctttgatt atgctggaat cagcgttgcc atgggaattt ctcatgataa aatcaaagaa
    26401 aaagcagatt atattacaaa aacattagaa gaagatggca tttttgatgc cttagaagta
    26461 tttggtatgg tagaaaaaga attgcatttt ccacaagtag acattgaaac agtagaaggt
    26521 cctcttgcga ctattaagac caatcacgga gacttacgta tcaagctttt ccctgaacat
    26581 gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag atggctacta tgatggtgtc
    26641 attttccacc gtattatcaa ggactttatg atccaaggtg gagacccaac tggaactggt
    26701 atgggtggcg agtcaatcta cggcgaatct tttgaggatg aattctcaga agagctttac
    26761 aatatccgtg gtgctctttc catggcaaat gctggtccaa ataccaacgg cagccagttc
    26821 tttatcgtgc aaaaccaaca cctaccttat tctaagaaag aaattactcg tggtggttgg
    26881 ccagaaccga ttgcagaaat ctatgccaat caaggtggga cacctcacct agaccgccgt
    26941 cacacggttt ttggtcagtt agctgatgaa gcatcttacg ctgtcttgga tgtcattgct
    27001 gctgttgaga caggagctat ggacaagcca gttgaagatg ttgtaattga aactattgaa
    27061 atcgaggact aagatgaaaa tcggtgataa gctaaagggg cgtattacag ggattcagcc
    27121 ctacggtgcc tttgttgagt tagagacggg tgatacgggg ctgattcaca tttcagaaat
    27181 tcggacagga tttattgaaa atattcacga aaccttgaaa gtcgatgaag aggttcaagt
    27241 tcaggtagtg gatttagatg aatttacggg gaaagccagt ctttctatcc gcactttgga
    27301 ggaagaaaag taccagtttc ctagacggag acgcttttca agcgaccgct ttaactacgg
    27361 ttttgctccc tttcgacgaa tgctaccaat ctggactgga gaagccctgc accatttaaa
    27421 aaagaagaag tagtttagag ttttatattt tttgtaatgt taatatgaat ttgctataat
    27481 aggaccatgg aagaagaaca attattaaaa tcaggagagc gcattaacca gctcttttcg
    27541 acagatatca aaatcattca aaatagagag gtttttagct attcggtgga tagtgttctc
    27601 ttatcacgat ttccacgttt tcctaagaag gggttgattg tggatttctg tgctgggaat
    27661 ggagcagtgg ggctttttgc tagcactcgt actcaagcac agatattgtc tgttgagatt
    27721 caggagcgtt tggcggatat ggctgaacgc tctgtccgtt tgaatggatt agaggagcaa
    27781 atgcaggtca tctgcgatga tttgaaaaat atgcctgctc acattcaggg gagtaaggtg
    27841 gatatgattt tgtgtaatcc accctatttc aaggtgaatc cttattctaa tctgaacgag
    27901 agtgaacatt atctcttggc tcgacacgaa atcacgacta atttggaaga aatctgtcgt
    27961 agtgcccaga gtattctcaa gtctaatggg cgtttggcca tggttcatcg tcctgatcga
    28021 cttctggata ttttggatac gttaaaacga cataatctag cccctaagcg cctgcagttt
    28081 gtttatccaa aaagagaaaa ggaagccaat atgcttttga ttgaggcgat caaggatggc
    28141 tcaacaagtg gctttaaggt cttacctcct ttgattgtcc acaatgatga tggctcttat
    28201 acgcccgaaa tcgaagagat ttattatgga tcataaggct tatatgtacg ttctggagtg
    28261 tcgtgatgga tcctactata taggctatac gactgatatg agaagacgcc tcgctatcca
    28321 caatagtggg aagggagcca aatatacacg agcacgcttg ccagtcaaac ttatctatgc
    28381 tcaaggtttt gccagtaagg aagaagccat gtcggctgaa gctcttctca agcgtaagaa
    28441 gaggccacag aaggaagaat ttttatctga aaatcaagat agaaatttac tcagttattt
    28501 tgaagaatcg tggggagtcc tttgactcct cttacctttg tcaaaaagcg aaaaaaatgc
    28561 tacaataaag aaaataaaca aaatgaggta ttatcatgtc caagattcta gtatttggtc
    28621 accaaaatcc agactcagat gccatcggat catctgtagc ttttgcctac cttgcaaaag
    28681 aagcttacgg tttggatacg gaagctgttg cccttggaac tccaaatgaa gaaacagcct
    28741 ttgtcttgaa ctattttggt gtggaagcac cacgtgttat cacttctgcc aaagcagagg
    28801 gggcagagca agttatcttg actgaccaca atgaattcca acaatctgta tcagatatcg
    28861 ctgaagtaga agtttacggt gttgtagacc accaccgtgt ggctaacttt gaaactgcaa
    28921 gcccacttta catgcgtttg gagccagttg gatcagcgtc ttcaatcgtt taccgtatgt
    28981 tcaaagaaca tggtgtagct gtgcctaaag agattgcagg tttgatgctt tcaggtttga
    29041 tttcagatac ccttcttttg aaatcaccaa caacacaccc aacagataaa atcattgctc
    29101 ctgaattggc tgaattggct ggtgtaaact tggaagaata tggtttggca atgttgaaag
    29161 ctggtaccaa cttggctagc aaatctgctg aagaattgat tgacatcgat gctaagactt
    29221 ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa cacagttgac atcgctgaag
    29281 ttttggaacg ccaagcagaa attgaagctg caatgcaagc tgccaacgaa tcaaacggct
    29341 actctgactt tgtcttgatg attacagata tcgtcaactc aaactcagaa atattggctc
    29401 ttggtgccaa tatggacaag gtcgaagcgg ctttcaattt caaacttgaa aacaatcatg
    29461 ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt acctcaatta actgaaagct
    29521 ttaatgcgta agattttggg tgtcagctca aaatcggaaa gtctagtttg ccttatatcg
    29581 aaaggagttt cggctccttt ttttctagga gtgaagtatg ttagaaaatg gcgatttgat
    29641 ttttgtgaga gatgggtcag acatgggaca ggccatccag acttccacag gtaactatag
    29701 ccatgttgcc atttatttgg atgggatgat ttatcatgct agtggacagg ctggtgttgt
    29761 ctgtcaagaa ccggcagact tctttgagtc caatcattta tacgacctct atgtttaccc
    29821 agaaatggat atccagtcgg tgaaggaaag agcttgcaga catcttggag caccctacaa
    29881 tgcttctttc tatccagatg cagctggttt ctactgctcc cagtatatag cagaaatcct
    29941 acctattttt gaaactattc ctatgaaatt tggagatggg gaacaggaga ttagtgattt
    30001 ttggagggag tattacatag aactaggtct gcctgttcct ctgaaccaag ctgggaccaa
    30061 tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa gaaaggaatc ttcatgattc
    30121 agatttttaa tccatctcgt ttgacgagac agccattttt ggagaattga tccgctatct
    30181 ggaccagtat gaggatgtga ttctacggga aattaaggct caatttccag atgttgcagt
    30241 tgataaactc atggaagagt atataaaggc aggcttgatt ctacgtgaaa ataagcgcta
    30301 ttacctcaat tttcctacgc ttgaatcact tgatagtctt gaactggatc aagagatttt
    30361 tgtcagagaa gctagtccgg tctatcaagc cttgttggag cagagttttg agacggaatt
    30421 gcgcaatcaa atcaatgcag ctattttagt tgaaagacgg actttgcgcg cattaaaatg
    30481 accctgtcca attattttta caaggtcaaa cagcagtatc ctttgacaga aaaacagcag
    30541 gagctctatg acattttagg agatgttaat cctgagtatg ccctcaagta tatgacggct
    30601 tttttgttga aatttctcaa aaaagaccag cttatgcaga aatgccgtga tatctttgtg
    30661 gacagtttgg ttgtcttagg ctatattgtg caaaatgaag atagaaagta tgagttggct
    30721 atcgattttg ataaggagag attaactttc tacttagcgt gatttcttgt ttctgagcac
    30781 attgtttgac tttccttagt attcggtata aactatatgt aaccggtaac acatatcgga
    30841 ataaactaaa ggagacaatc atatgtcact tgaaaacaaa ttggaacaag caacaggcgc
    30901 tgtcaaagaa ggttttggta aagttactgg agacagcaag acagaacttg aaggagctgt
    30961 tgaaaaaaca gttgctaagg caaaagacgt tgtagaagac gcaaaaggtg ctgtagaagg
    31021 tttgaaaaac gtttttacta aataatagga aaaaatcaag ggtttcattt tcccttgatt
    31081 ttttctattc ttataaataa ttttctgcga cggctgtatc tcctgggtag gattctttct
    31141 tgccctggat gatttggtaa caatcggctc ccttacccgc aataataact gcatctaatt
    31201 catgatttgt gatagccatt gccgccttga tggcttcttg gcgatccgca atcttttcaa
    31261 caggatgatt gatgtagcta ctaatttcat ctgcaatggc cattgggtct tcatagttag
    31321 ggtcatcagc agtcagaaag acttgaatct caggctgttg attgaggagg aggccaaagt
    31381 ccttacgacg actttctccc ttgtttcctg ttgatcccag aaccagagca atctttccgg
    31441 tttgatgagt ttccaccaca ttgatgagtt ttttcagact atccccattg tgggcatagt
    31501 cgatgaagac cttggctcca tttttctgag tgaggacttc catacgacca ggaacgcggg
    31561 ttgcagcgat gccttttttg atgtcctcaa gacttgctcc gagacggaga caagcaagtc
    31621 cagcagcaac tgcattttct tggttgaagt tgccaatgag ttggatatca taatctccag
    31681 cgagtttacc tgtagctgaa aagctaaagg ctttggaatt ctcgatttgg ttatcaaatt
    31741 ggctaccata gaaatcatgg tcttgatctt caacctgttc tctcaagact gagaagtggt
    31801 ccatgtcact gttaatgatg actgctcggc tattttccat caagagacgc ttgtggtaga
    31861 aatagtcttc aaagctaggg tgttcaatcg ggccgatatg gtctgggctg atatttagga
    31921 aaactcccac atcaaaggtt agaccataga cacgtttgac cagataggct tgactggaga
    31981 cttccatgat gaggtgggta cggtcatttt gcacagcctg attcatcatg tcaaagaggt
    32041 caatactctc aggggttgtc aacgctgact taaagaaagt ctcgccatca agagttgtgt
    32101 tcatggtcga caacatagca ggtctatgcc cttgagataa gatgttatag gcgaaatagg
    32161 ctgctgttgt cttaccctta gtaccagtaa aggcaaggag tttgagtttt tcctgtggat
    32221 taccatagaa ctccatggca atcaaactca tggctttctt tatatcgttc acaatgatga
    32281 cagggatacc gacttcgtag tccttttcag ctacatacca agctaatcct tgtgttatag
    32341 cagaaagaag gtattctttt ttaaaggcag cgccttttgc aaaaaaaaga gtgtcttctg
    32401 ttacttttcg gctatcgtag ctgatgctat caaaaacaac tttgctgtag ttgtagtggt
    32461 aatgaccttg gtcaataatt tcgcgaaaaa ggccatcttt ctttaaaata tctaatacgg
    32521 tttcaatctt aatcatactt tctattgtaa accgaaagtc gtaaatttac aagtaacaag
    32581 gaaaagttta taatggaaga taaggagttt ttcctagtta tcaaaattga atgaggaatc
    32641 tatgtcgcac gaaaacaatc accagcaggc ccagatgtta cgggggactg cttggctaac
    32701 ggctagtaac tttatcagtc gcctactcgg ggctgtttac attatccctt ggtacatctg
    32761 gatgggggct tatgcagcta aggcaaatgg tctctttacc atgggttaca atatctatgc
    32821 ttggttcttg ttggtttcaa cagcggggat tccagttgcg gtggccaagc aagttgccaa
    32881 gtataatacc atgcgagaag aagagcatag ctttgccctg attcggagct tcttaggctt
    32941 tatgacagga ctaggcctgg tttttgcttt agtcttgtat gtctttgctc cttggctagc
    33001 agacttgtct ggcgtgggca aagacttgat cccaatcatg caaagcttgg cttggggagt
    33061 cttgattttc ccgtctatga gtgttatccg aggatttttc caagggatga ataacctcaa
    33121 accctatgcc atgagccaaa ttgctgagca ggtcattcgt gttatctgga tgctcctagc
    33181 aacctttatc attatgaagc tcggttcagg agattatcta gcagccgtta cccaatcaac
    33241 ctttgctgcc tttgtcggta tggtagccag ttttgcagtc ttgatttatt tccttgccca
    33301 agaaggttca ctcaaaagag tctttgaaac aggagataag attaacagta agcgtctctt
    33361 ggttgatacc attaaggaag ccattccttt tatcctgaca gggtctgcca tccagctctt
    33421 ccagattttg gatcagctga cctttatcaa tagtatgagc tggtttacca actacagcaa
    33481 tgaggacttg gttgtcatgt tttcttattt ctcagccaat cctaataaaa tcacgatgat
    33541 tttgatttct gtaggggttt cgattgggag tgttggtttg ccacttttga cggaaaacta
    33601 tgtcaagggg gacttgaaag cggcttctcg tctcgttcag gacagtctca ccctactctt
    33661 tatgttcttg ctaccagcaa cggttggagt ggttatggta ggagaacctc tttatacggt
    33721 cttctatggt aagccagata gtttggctct gggcttattt gtctttgcag ttttgcagtc
    33781 tattatttta ggcttgtaca tggtcttgtc tccaatgctt caggccatgt tccgcaaccg
    33841 caaggccgtt ctctatttta tctatggttc tattgccaag ctagtcttgc aactacctac
    33901 catcgccctc ttccacagtt atggtccttt gatttcaaca accattgctc tcatcattcc
    33961 taacgtcttg atgtatcggg atatttgtaa agtaactggt gtcaagcgca aggtgatttt
    34021 gaagcgaacc attttaatca gtttgctgac cctagtcatg tttctgttaa taggaaccat
    34081 ccagtggctg ttaggatttt tcttccaacc aagtggacgt ttgtggagct tcttttatgt
    34141 agctcttgtc ggtgccatgg ggggtggact ttatatggtt atgagtctgc gtacctattt
    34201 attagataag gtaataggaa aagcccaagc agatcgcctg cgagcaaaat ttaagctttc
    34261 gtaaggtagc atttataaat aaaaggaata agttgaacaa tcttttagga gaacttgaaa
    34321 cttattcctt ttttagcgtc aaaatagcta aaattatctt tcaaaaatag aaaatgaggt
    34381 ttttttgatt ttttggaaaa aacaattgac taaacaaacc aagagttgta taataaaaca
    34441 aatatttaaa gcaggaggtt ctggaaatga aaaagtctaa gagcaaatat ctaaccttgg
    34501 caggtcttgt cctgggtaca ggagttttat tgagcgcgtg tggaaattct agcacggcgt
    34561 caaaaaccta caactatgtt tattcaagtg atccatctag cttgaactat ctagcagaaa
    34621 accgcgcagc aacatccgat attgttgcaa atttggtaga cgggttatta gaaaatgacc
    34681 aatatgggaa tattattcca tcattagcag aggattggac tgtttctcag gacggtttga
    34741 cctataccta caaacttcgt aaggatgcca agtggtttac ttctgaggga gaagaatatg
    34801 cgcctgtaac tgcccaggat tttgtgacag gtttgcaata tgcagctgat aaaaaatcag
    34861 aagccttgta tctagtgcag gactctgttg ctggtttgga tgactatatc actggtaaaa
    34921 caagcgactt ttcaactgtc ggtgtcaagg cacttgatga ccaaacggtt caatatactt
    34981 tggttaaacc agaactttac tggaattcaa aaacacttgc aacgatactt tttcctgtta
    35041 atgcagattt cctgaaatca aaaggggatg attttgggaa ggcggatcca tctagtattt
    35101 tgtacaatgg acctttcttg atgaaagcac ttgtctcaaa atctgctatt gaatataaga
    35161 aaaaccctaa ttactgggat gctaagaatg tctttgtaga cgatgtgaaa ttgacctact
    35221 atgatggtag cgaccaagaa tcactggaac gtaattttac agctggtgct tatactacgg
    35281 ctcgtctttt tcctaacagc tccagctatg aagggattaa agaaaaatac aaaaacaata
    35341 tcatctatag tatgcaaaat tcaacttcat atttctttaa ttttaaccta gataggaagt
    35401 cttacaatta tacttctaaa acaagtgaca ttgaaaagaa atcgactcag gaagcagttc
    35461 tcaataaaaa cttccgtcag gctatcaatt ttgcttttga cagaacatct tatggggctc
    35521 agtctgaagg gaaagaaggt gcaacaaaga ttttgcgtaa cctagtggtt cctccaaact
    35581 ttgtcagtat caagggaaaa gactttggtg aagttgtagc ctctaagatg gtcaactatg
    35641 gtaaggaatg gcaaggtatc aactttgcgg atggtcaaga cccttactac aatcctgaga
    35701 aagccaaggc taagtttgcg gaagctaaga aagaactcga agcaaagggt gttcaattcc
    35761 caatccactt ggataagact gtggaagtaa cagataaagt aggcatacaa ggagttagtt
    35821 ctatcaaaca atcaattgaa tctgttttag gttctgataa tgtagtgatt gacattcagc
    35881 aattaacatc agatgagttt gacagttcag gctactttgc tcaaacagct gctcagaaag
    35941 actatgattt atatcatggc ggttggggac ctgattatca agacccgtca acctatctcg
    36001 atatttttaa tactaatagt ggaggagttc tacaaaatct tggactagag cctggagaag
    36061 ccaatgacaa ggctaaggca gttggactgg atgtctatac tcaaatgttg gaagaagcta
    36121 ataaagagca agatccagcc aaacgttatg agaaatatgc tgatattcaa gcttggttga
    36181 ttgatagttc tttagttctt ccaagtgttt cgcgtggggg aacaccatca ttgagaagaa
    36241 ccgtaccatt tgctgctgcc tatggtttaa ccggtacaaa aggggttgaa tcatataaat
    36301 acctcaaagt acaagataag attgtcacaa cagacgaata tgcaaaagcc agagaaaaat
    36361 ggttgaaaga aaaagaagaa tccaataaaa aagcccaaga agaattggca aaacatgtca
    36421 aataaaatct aattgtagat aagtttgtgt aagaagtctt tgtgcttaaa aacgcataat
    36481 atcaggtata taaaatccta gatgttatgc gttttgttat aggaagattt attagatttt
    36541 atcctcaaat tgagttattc ccgactttat ccgttgaaat attataagga gtatttatga
    36601 aattaaaatt attaagagta gatactaagg tgattatggg gagtttctta cttgttctgt
    36661 ctagtctact tgctttgttg cttcccctta tcttaaaggg tttaatagat gggagttcta
    36721 ttgaaaatat aggttccaaa gtatttcaat cgtttttgat ttttattggt caagccttgt
    36781 tttcttctat tggttactat ctgtttagtc aatcgggtga aaaaaagata gcaaaaatca
    36841 ggaaaaaagt gatagagggg ttgatttatg cagagaaatc cttctttgat aagagccaaa
    36901 gtggggagtt gacttctgcc attgtcaatg acatgagtgt cattcgtgag tttttaatta
    36961 cgactttccc aaatattatt ctgagtttag ttatggtact tggttccatt gtagtcttat
    37021 ttagtcttga ttggaatctt tctctacttt tattcatcac tcttccttgt atgatgttta
    37081 ttatcttgcc cctttccaat atcagtgaaa agtatagtcg tcgtttacag gaggaaattt
    37141 aacagggcaa ttgactgaaa agattcaaga acatgaatta attaagacta accaagcaga
    37201 gaaaagtgta caggatgttt tggataattg tattgaaagg gtacaaaaca attcactgaa
    37261 atcagatagg gttacttctt ttgagacccc gtttgctctc ttatttatct ttgcgactat
    37321 agctgtgatg ctaacctatg ggggttatcg ggtcagcgca ggatatatat ctgtgggaac
    37381 cttggtttcg tttttgattt acctctttca attacttaat cctattagta atatagctaa
    37441 ttttgtaact gtttattcta ggagcaaggg atcttcagtt gcactggata acttgcttgc
    37501 agttcctaaa gaaaaatttg agggaggaaa atcggtatca ggacaagggt tgaattttaa
    37561 ccatgtctat tttggttatg atgaaaatcg acctgtctta aaggatatta cttgttcaat
    37621 tttcaagggg caaaaattgc ttttgttgga ccatctggat caggaaaatc aacgattgtg
    37681 cgtttgttag agcggtttta taaaccgctt tcaggagata ttctaatgga gcaatcaagt
    37741 atatatgatt ttaacttaaa agaatggaga agtaaaatcg cttgggtttc acaaaataat
    37801 gcagtcttat ctggcagtat tcgtgacaat ctttgtctcg gtttgaatcg cttagtaatt
    37861 gatgatgaat tgatgaaagt gctagactta gtatcactag gtgatgagat tcgctccatg
    37921 aaagagggac tagatactga agttggtgaa cgcggacgat tcttgtcagg ggggcaaagc
    37981 caaagacttc aaatagctag aacctactta aaagatgctg aaattcttat atttgatgaa
    38041 gctactgcta atcttgatgc ggattctgag tatgcgatta tcagtagcct ctattctgta
    38101 ttaaaggaga agacggttgt gattatagcg catagtttgt caacggtaaa agatgtggat
    38161 tgtattttct tcttagagga ggggaaaatc actggctcag gaactcataa ggaactactg
    38221 gaaaatcatg agcgttatgc tcgttttgtg caggagcaaa tgatagagtg aagtgtcttt
    38281 tgagattcac cattttatag tctattaaag ggagcaggaa aaactccctt tttatatagt
    38341 ttgaaactat aactagctct tgaaaagaag aaaatgagtt gatgaaaata agtggtacaa
    38401 tagttactat agatttggag gtattgtatg agcaaggaat tacacattaa cacaattttg
    38461 gcccaggcgg gtattaagtc agatgaagcg acaggtgcat tggtgacacc gcttcatttt
    38521 tcaacgacct atcagcatcc agagtttggt cgatctactg ggtttgacta tacgcgcact
    38581 aaaaatccaa ctcgtagtaa ggctgaggaa gtcttggcga ctattgagtc agcagactat
    38641 gccttagcga ctagctcagg gatgtcagct attgtactgg cctttagcgg ctttccagta
    38701 ggaagtaagg tcttggcagt gcgtgatctt tacggcggtt cttttcgctg gtttaaccaa
    38761 gtggagcagg aaggtcgttt ccattttacc tatgccaata cagaagaaga gttgattgcc
    38821 gagttagaaa aggatgtgga tgttctctat atcgaaaccc caaccaatcc cttgatgttg
    38881 gaatttgata tcgaaaaact agcaaaattg gctcatgcta agggtgccaa agtggtggtg
    38941 gacaatacct tctatagccc tatctaccaa cgtccgattg aagatggagc agatatcgtt
    39001 ctccattcag caaccaagta tctagcaggc cacaatgatg tcttggctgg agtggttgtg
    39061 accaatagtt tagaactata cgagaagctt ttttacaatc tcaatacaac aggggcagtc
    39121 ttgtctccat ttgacagcta ccagttgctt cgtggtctca agaccttgtc tcttcgtatg
    39181 gagcgctcaa cagctaacgc ccaagaagtg gttgcctttt tgaaggattc tccagcagtt
    39241 aaggaagttc tctacactgg tcgtggaggc atgatttcct ttaaagtagc cgatgaaaca
    39301 cgcattcctc atattttgaa cagtctcaag gtcttctctt ttgcggaaag tttgggcgga
    39361 gtggaaagtc ttattactta tccaacgact caaactcatg ctgatattcc agcagaagta
    39421 cgccattctt atggtttgac agatgacctc ttgcgtttgt ctattgggat tgaggatgct
    39481 agagatttga ttgcagattt gcgccaagcc ttagaaggat aagacaaaga tgggaaaata
    39541 tgattttaca agcctgccca accgtttagg gcaccatacc tataaatgga aagaaacaga
    39601 aacggatagt gaagttctac cagcttggat agcggatatg gactttgtgg tcttgcctga
    39661 aatccgccaa gccgtgcaaa cttacgcaga ccaactggtt tatggttata cctatgccag
    39721 tgaagactta attaaggaag ttcaaaagtg ggaagctaca caatacggtt acaactttga
    39781 caaagaggct cttgtcttta tcgagggtgt ggtaccagcc atctcaacag ctattcaaac
    39841 ctttacaaaa gaaggcgagg cggttttaat taacacgcct gtctacccac cctttgctcg
    39901 cagtgtcaag ttgaataatc gtagattgat tactaattcc ttagtggaaa aggatggtct
    39961 gtttgagatt gactttgacc aacttgaaaa ggatttggtg gaagaggagg ttaaactcta
    40021 tattctttgc aaccctcaca atcctggtgg acgtgtttgg gaaaaagaag tgttggagaa
    40081 gattggccaa ctctgccaaa aacacggtgt tttgttagtt tcggatgaga ttcaccaaga
    40141 tttgaccctc tttggtcaca aacaccagtc tttcaatacc atcaatcctg ccttcaaaaa
    40201 ttttgctatc gtcttgagca gtgccactaa aacatttaat attgctggaa caaaaaattc
    40261 ctatgcagtc attgaaaatc ctaagttgag actagctttc cagaaacgcc tgttggccaa
    40321 taatcagcat gaaatttcag gcttgggtta tttggcgaca gaagctgcct atcgatacgg
    40381 taaagattgg ctagaggaac tcaagcaagt ctttgaagac cacatcaatt atgtggtgga
    40441 tctatttgga aaagagacta aaatcaaggt catgaaaccg caaggtacct acttgatttg
    40501 gcttgacttt tcagcttatg acctgactga tgaaacattg caagagttgt tgagaaatga
    40561 agccaaggtt atcctcaacc gtggtttgga ttttggagag gaaggaagtc tccatgcccg
    40621 catcaatata gctatgccca aatctctgtt gcaagaagtc tgtcagcgga ttgtggctac
    40681 ttttgccaaa cgttaaaaat ccagccttct aggagaaaag tcttcctaga aggctatttt
    40741 cataggcgaa aatatggtat aataaaaaga taaggtaaag gtgaaaatat ggctaaattg
    40801 attccgggga aaattcgtat cgaaggtgtt gccctttatg aaactggtaa ggttgatatc
    40861 atcaaggaaa agaacaatcg gctctacgct cgcgttgcag aagaagaact gcgctatagt
    40921 ttagaggatg atttggtttt ttgtgcctgt gatttttttc aaaagagggg ttactgtgtg
    40981 catttggcag cgctagagca ttttctgaaa aatgatgagc gtggtcagga aatcttgtgg
    41041 agtctggaag aaggtcatga agaaaaagag gctgttgaaa ccaaggtgac cttgggtggc
    41101 aagtttttgg atcgaatttt atctccaaaa tcagaatgcg cctatgagtt atcagctgtg
    41161 ggtcaggtgg aagcaggaac caatcatatc ttgtggactc tgagaatcgg tcaaatcaat
    41221 agccaaaaat actatgtcat tcgggatatt ccactttttt taaggattgt cgagcagaga
    41281 aagtcgtata tgattggcaa gatttatgag gagtctcttt cttgggaagc ctttgatgat
    41341 gccagccaag aacttttgac tttcttgcac ggactaacgg aggaagggct ggtttcagac
    41401 ctctttttcc aaaaccaagg tcggcacctc tttttccctc tgaccttttt tgagcagggt
    41461 gtgaatttac tgatggcctt gccccatttt caatttgacc atcaagtgga tagctaccaa
    41521 accctgcttt ttcaggatat gcatgctgat gccaatctat ttgcctttac agtaacagaa
    41581 tactcggact attttgaaat ggaaatcagt gagagtccta gggtcaatgt cttttatcag
    41641 ggagcagtac ttttccataa aggtcaggtt tattttttaa cagaccagca gatgcgtctt
    41701 ctcaaggaaa ttaaagcgtt acctgtgggt caacaaggaa agaaatacct gcaatttgat
    41761 agcagtgatc gagacaaatt ggcctcctgt ctgactcttt ttatccagat gggcaccgtt
    41821 tcagctccag aacgtctaca aatcaaaact tttgcgccct ctttttactt tgacagggaa
    41881 gaagataatc ggattcgttt agaaattcag tttgattatg gaaatagaca ggtatctagc
    41941 cgacaagagc tagaagaatt accattttca agtgacgcgg acttagaaga aagaattttc
    42001 caagtctgtt tggctgcggg ctttgaagcc gattttcaat cttggcgtca ggccttaaaa
    42061 gcggagtctg tctatcattt ttttcatgat atcattccag tctttgaaaa actcgggcat
    42121 gttgacctat cagataaact agaagagctt tatagcctag cgagtcctca ggtgcagatt
    42181 gcctccaagg gaggtctctt ggaaatccag tttgattttc aagatattgc ccaggaggaa
    42241 atcgaccaag ccatgcaggc cttggttgcc aatcaggatt tttatattga ttcgtctaat
    42301 caagtttact ttttcgatga agaaaccaag aaaattcgcc aaaatctaca ggaactgggg
    42361 caatttgaat taaaagatgg gaccttgcag gctcggaaat ccttggccta cagtttagct
    42421 catctctttg aagggcgaga ccgtgtttct ttttcacaag aattccagaa tatggcccag
    42481 gatttgacac atccagagga ctttcctcga caggcaactc aggttcaggc tgacttgcga
    42541 gattatcagg aaaagggaat tggctggttg cagatgctcc atcattatgg ttttggtggg
    42601 attttggctg atgatatggg acttgggaaa acccttcaga ccattgcttt tttgactagt
    42661 caagtgacaa aagaaagtcg ggttttgatt ctcgctccgt caggtttgat ttacaactgg
    42721 gcagatgagt ttcagaaatt tgccccacag ttggatgtgg ctgttgttca tggtttgaaa
    42781 gcaagtcgtg aggagattct tgccgagagc catcaaatct atgtgactag ctatgccacc
    42841 ttccgtcagg acagtgagct ttatcagggg atggcctttg acttcctttt cttagatgaa
    42901 gctcaggtca tgaaaaatgc ccagaccaag attgcccaga ccttgagaca atttgtggtg
    42961 ccgtcggtct ttgccttgtc aggaactccg attgaaaacc atctgggtga gttgtggtct
    43021 attttccaaa tcgtcatgcc cggacttttg ccaagcaaga aagaatttat gaaattgcca
    43081 gcagagcgag tggctcagtt tatcaagcct ttcgtgatgc gacgcaagaa agaagaagtt
    43141 ctgactgaat tgccagactt gattgaggtg gtttataaaa atgaactgga agaccagcaa
    43201 aaggctattt accttgccca gttacaacag atgcgagacc atctggctca agtgtcagaa
    43261 caggaatttc agcgaagtcg tgtggaaatt ttatctggtt tgatgcgctt gcgtcaaata
    43321 tgtgacactc ctgccctgtt tatggaagat tatcagggag ccagcggcaa actagatagt
    43381 ctccgagacc tactggtaca ggtggcagac ggcggacacc gtgtcttgat tttctctcag
    43441 ttcaagggaa tgttggaaaa aattgaacaa gaactgccag acttgggcct gacttccttt
    43501 aaaattacgg gttcaacccc agccaaggaa agacaagaca tgaccaaggc ctttaaccaa
    43561 ggagaaagag atgcctttct gatttccctc aaggctggtg gggtcggtct gaacctgaca
    43621 ggtgcagata cagtgatctt ggttgacctt tggtggaatc ctgcggtgga agcgcaagcc
    43681 attggccgtg cccatcggat gggtcaggaa gaaacggttg aggtctatcg cttggtgacc
    43741 aagggaacca ttgaagaaaa aattcaggaa ctccaagaac aaaagaaaca tctggtgtca
    43801 caagtattgg acggcacaga gtcacgtggt agtctgaccc tagcagaaat tagagaaatt
    43861 ttgggaattt ctgaagccag cacttgaaaa atcaagacaa tacgctataa tgaagtgttg
    43921 aaaggaaata gaaaatgaaa caagaacgat ttccattggt gtcagatgac gaggtcatgt
    43981 tgactgaaat gccagtcatg aatctctatg atgagtctga tctgatcagt aatatcaagg
    44041 gtgagtatcg agataaaaat tatttagaat gggctcctat tgctgaagaa aaaccagtaa
    44101 aaccgattga aaagcaagtc gaaaaaccta aaaaggctcc tttaggggtt aaaaaagaag
    44161 gaaagagcta tgcggaggtg gcgcgtgaag aagcgcgtgc ggacttgaaa aagaaacgct
    44221 ctgctaacta cctaactcag gatttcagcc ttgcgagacg tcattctcag cccagtctag
    44281 ttagacaggg caatcaaccg acaactcctt tccaaaagga aaatcctggt gaatttgtca
    44341 aatatagcca aaaattgacc cagtctcatt atatcttggc ggaagaagtt cattctatcc
    44401 ctaccaagaa tgaagaagtg tcagcacctg ctccaaagaa aaacaattat gattttctaa
    44461 agaagagcca aatctacaat aaaaaaagta aacaaacaga acaagaacgt cgggttgccc
    44521 aagagttgaa tctgaccaga atgacagaat aggggagaaa acatgtcaaa gacatatcat
    44581 tttatcggaa ttaagggatc agggatgagt gccttggcct tgatgttgca ccagatgggg
    44641 cacaaggttc agggatcaga tgttgaaaag tactacttta cccaacgcgg tcttgagcag
    44701 gcaggaatta ccattcttcc ttttgatgaa aagaatctag acggtgatat ggaaattatc
    44761 gctggaaatg cctttcgtcc agataacaac gtcgaaattg cctatgcgga ccaaaatggt
    44821 atcagctaca aacgttacca tgagtttcta ggtagcttta tgcgtgactt tgttagcatg
    44881 ggagtagcag gagcacatgg aaaaacttca acgacaggta tgttgtctca tgtcttgtct
    44941 cacattacag ataccagctt cttgattgga gatgggacag gtcgtggttc ggccaatgcc
    45001 aaatattttg tctttgaatc tgacgaatat gagcgtcact tcatgcctta ccacccagaa
    45061 tactctatta tcaccaacat tgactttgac catccagatt atttcacaag tctcgaggat
    45121 gtttttaatg cctttaacga ctatgccaaa caaatcacca agggtctttt tgtctatggt
    45181 gaagatgctg aattgcgtaa gattacgtct gatgcaccaa tttattatta tggttttgaa
    45241 gttgaaggca atgactttgt agctagtgat cttcttcgtt caataactgg ttcaaccttc
    45301 accgttcatt tccgtggaca aaacttgggg caattccaca ttccaacctt tggtcgtcac
    45361 aatatcatga atgcgacagc cgttattggt cttctttaca cagcaggatt tgatttgaac
    45421 ttggtgcgtg agcacttgaa aacatttgcc ggtgttaaac gtcgtttcac tgagaaaatt
    45481 gtcaatgata cagtgattat cgatgacttt gcccaccatc caacagaaat tattgcgacc
    45541 ttggatgcgg ctcgtcagaa atacccaagc aaggaaattg tagcagtctt tcaaccgcat
    45601 acctttacaa gaaccattgc cttgttggac gactttgccc atgctttaaa ccaagcagat
    45661 gctgtttatc tagcgcaaat ttatggctcg gctcgtgaag tagatcatgg tgacgttaag
    45721 gtagaagacc tagccaacaa aatcaacaaa aaacaccaag tgattactgt tgaaaatgtt
    45781 tctccgctcc tagaccatga caatgctgtt tacgtcttta tgggagcagg agacatccaa
    45841 acctatgaat actcatttga gcgtctcttg tctaacttga caagcaatgt tcaataggat
    45901 gttcccatgg aaattccaat taagatcatt caggcaagca agtctgattt gcctgagata
    45961 ggggcacttc agacctcgtc ttttccagct gaaaagcagc aactttccca tattttagaa
    46021 gaaagtatcc gtaagtgtgc ggataccttt cttctagcta gggatgaaaa tcaactttta
    46081 ggctatattt tatcaagtcc ccagtcagac aatccgcaat atctaaaagt acattcttta
    46141 gtcatcgagt ctgaccatca gagacagggc ttgggaacac ttcttcttgc agccttgaaa
    46201 gagatggcag ttgagctgga ttacaaaggg attcgtttgg agagtcctga tgagctgctt
    46261 tcctattttg aaatgaacgg ttttgttgat gaagaagcaa ctttgctcta tgcaactagc
    46321 cagggctata gtatgatttg gtttaatccc ttttatctgg aggaacaatg aaaatcagac
    46381 aagcaagatt agaagatttg gatcggattg ttgaacttga atttgaaaat ttttcggtcg
    46441 aagaagccat tcctccttct gtctttgaag cacatttgag agaaattcag acctcttttc
    46501 tggttgctga aaaagaagga agaatcatgg gttatatcga aggaccagtt ggcctgcacc
    46561 gccatctgca agaccagtct tttacagaag aaataaaaga ctatagtcat gagcctggtg
    46621 gttatatctc tgtgacctgt ctgtctattg ccaaggaggc acagggactc ggactgggtc
    46681 agaaattgct gacagccttg aaagaagttg ctcttgaaga tgagagagat ggcattaatc
    46741 taacctgtca tgactatctc atcgcctact atgaaaaaca tggatttgtc aacgaaggcc
    46801 agtcccagtc aacctttgca ggggaaacat ggtatgatat ggtctgggaa atgaaaaaat
    46861 aagttaggaa aagtatcata gattgctttt tttggacttt taagatataa tattatggat
    46921 tatcaacaag gaggaaaaac ttttgagtga aaagtcaaga gaagaagaga aattaagctt
    46981 taaagagcag attctgagag atttagaaaa agtaaaaggc tatgatgaag ttctgaaaga
    47041 agatgaggca gtagttcgca ctcctgcaaa tgaaccttca gctgaagaac tcatggctga
    47101 ttccttgtca acggtagagg agattatgag aaaagctcct accgtgtcta ctcacccaag
    47161 tcaaggtgta ccagcttctc cagcagatga gattcaaaga gaaactcctg gtgttccaag
    47221 tcatccaagt caagatgtac cttcttctcc agcggaagaa agtggatcaa gaccaggtcc
    47281 aggtcctgtt agacctaaga aacttgaaag agaatacaat gaaaccccaa caagggtagc
    47341 tgtttcctat acgacggcag agaaaaaagc agaacaagca ggtccagaaa cacctacgcc
    47401 tgctacagaa acagtggata tcatcagcga tacatcacgt cgtagccgta gagaaggagc
    47461 aaaacccgtt aatcctaaga aagagaagaa gtcacatgtg aaagcttttg tgatttcatt
    47521 ccttgtattc cttgccttgc tctcagcagg tggttacttt ggttaccagt acgtgctaga
    47581 ttctttgtta cctatcgatg ctaattctaa gaaatatgtg acggttggaa ttccagaagg
    47641 ttcaaacgtt caagaaatcg gtacgacgct tgaaaaagct ggtttggtaa agcatggtct
    47701 gatttttagt ttttatgcca agtataaaaa ttataccgac ttgaaagcag gttactacaa
    47761 tttgcaaaag agtatgagta cagaagactt actcaaagag ttgcaaaaag gtggaacaga
    47821 tgaaccgcaa gaacctgtac ttgcgacttt gacaattcca gaaggttata ccttggatca
    47881 gattgctcaa gctgtgggtc aattgcaagg tgacttcaaa gagtctttga cagcggaggc
    47941 tttcttggct aaagttcaag atgagacgtt tatcagtcaa gcagtagcga aatatcctac
    48001 tttactggaa agtttgcctg taaaagacag cggtgcgcgt tatcgtttgg aaggatacct
    48061 tttcccagct acatactcta tcaaggaaag cacaactatt gagagcttga ttgatgagat
    48121 gttagctgct atggataaga acctatctcc ttactatagt actatcaaat ctaaaaattt
    48181 gactgtcaat gagttgttga ccattgcttc cttggtcgaa aaagaaggtg ccaagacaga
    48241 agatcgtaag ctcattgcag gtgtattcta caatcgttta aatcgtgata tgccacttca
    48301 aagtaatatt gcaatcttgt atgcccaagg aaaactgggg caaaatatca gtctagctga
    48361 ggatgttgcg attgatacca acattgattc accttataat gtttataaaa atgtaggtct
    48421 catgcctggt ccagtcgata gtccaagtct ggatgcgatt gagtcaagca tcaatcaaac
    48481 taagagcgat aacctctact ttgtagcaga tgtcacagaa ggcaaggtct actatgctaa
    48541 caatcaagaa gaccacgacc gcaatgtcgc tgaacatgtc aacagcaaat taaactaaac
    48601 aaactaaaat tatgtgatac gtcacataat tttctttaga aaatattatc agaagaaagt
    48661 tgagaaaaat ggcagaaaaa acatatccta tgacccttga ggaaaaggaa aaacttgaaa
    48721 aagaattaga agaattgaaa ttggttcgtc gaccagaagt ggtagaacgc attaagattg
    48781 cccgttcata cggtgatctt tcagaaaaca gtgagtacga agcagctaag gatgaacaag
    48841 cctttgtcga aggacaaatc tctagcttag aaacaaaaat ccgctatgct gaaatcgtca
    48901 atagcgacgc agttgcccag gacgaagtag cgattggtaa aacagtcacc atccaagaaa
    48961 ttggcgagga cgaagaagaa gtttatatta tcgtaggttc agctggtgcg gatgcctttg
    49021 caggtaaggt ttcaaatgaa agcccaattg ggcaggcctt gattggcaag aaaacaggtg
    49081 acacagcaac aattgaaacg cctgttggta gctatgatgt aaaaatcttg aaggttgaaa
    49141 aaacagccta aaaacagaaa aaggagtggg aaggcgatgt gcttcactca ctcctttttc
    49201 cattttgcta ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg ccgtatatat
    49261 ggttactgac ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag ctgacttcgt
    49321 cagttctatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt
    49381 gctctttgat tttcattgag tattagatgt atagtgcatt gaagttaaaa tagtaaactt
    49441 tgatttctaa gacattatta gaaattagat taaattcctt aatcgatttg cgcatgtttt
    49501 atttcatttc attatacttt agattggaaa ataaggaatc acataaatcg caagaaatag
    49561 ggaatctgat tcgtgtttat aaccgttcaa aaagggaaga ggctgaaagt gagccactta
    49621 atctttatgt cgaagatgaa aagggcaatc tccttgcggg tttgatagca gagacttttg
    49681 gaaattggct agaaatcgag tatttgtttg taaaagagga actgcgaggg caaggaatcg
    49741 gttcaaaact attgcaacag gcagaaagtg aagctaagaa tcgaaattgt tgttttgcct
    49801 ttgtcaatac ttatcagttc caggcaccgg acttttatca gaaacatggc tacaaggaag
    49861 ttttttcttt gtaagactat ctctacatta ggcaaagata ttattaccaa aagaatctgt
    49921 aaggtgagta tggaggtata atacaaagag aggctcgcac ataagtatgg aggtggactg
    49981 atgtatatga gtaactatcg ataaatacca gttataacct agaaattgtg tctttctaga
    50041 cgatattgag gacaatataa tcgtagctcc aaaattagga atcaaggctt atcaggttaa
    50101 gaaaagaagt gatgttgttg atattttaaa acaatttatt taaactaaaa actctctatc
    50161 tttttaaatt gacattaatc cgagattccc ctctcggatt ttgtgttgct ttcattaaat
    50221 ttacaggtaa gacttgctag cttcagtaaa tatacacaag tttttcagaa tagagtgaaa
    50281 tccgaaagaa aatggaaata atgaaagctc ttgtaggcat aagaaaacac ctctgtgtta
    50341 tacttgttgt tcaaccacaa acacaagaaa ggcacagaaa tgcaagacaa ttatactaca
    50401 aaaggtaaac atttgacaat cgatagccgt cgcttaatcg aaagatggaa aaaagaagga
    50461 aaatcaaata gagaaattgc ctctctactt ggaaaagctc ctcaaactat ccacactaaa
    50521 atcaagcgta ggacagtccg aaaatgtctt ggaaaagggc gcttcaaaga ggtttattct
    50581 gccgactatg ctcaacagtc ttatgaaaat aatcgcaagc actcggtcaa gagatcaagc
    50641 gtgaccaagg aactaaagga aaagattctc cactatcata accaaaaatt tttgcctgaa
    50701 atgatggtta tggctaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat
    50761 catggaaaat tggggtaaag caagcaggat ctgctttacc ctagaaaagg aaaagcgctt
    50821 aagaaacagg ccagcaccaa ctttaaacct gctggtcaat ccatcgaaca gcggcctgaa
    50881 gctatcaatt ttcgcttgga gaatgggcat tatgagattg atacggttct acttacgaga
    50941 gcgaaaaact actgcttgct tgtcttgacg gatcgaaaaa gtagacatca gatcatccga
    51001 ttgattccaa ataaaagtgc tgaggtggtc aatcaggctc taaaactcat cttaaaacaa
    51061 cacaagattc tttccatcac ggcagataat ggaacggaat tcaatcgctt gtctaatgta
    51121 ttttctgagg agcacattta ttatgcacat ccctatgcct cttgggaaag gggaactaat
    51181 gagaatcaca acaggctcat tcgtagatgg ttacctaagg gaaccaagaa aatgactccc
    51241 aaagaagtcg cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttggactac
    51301 aagtcaccca gagaagactt ctggatggct aacttgaact tgaaatttag caaaatggaa
    51361 ataattttta ttaaacgctt ccaatagcgg ttaaaagttg acaaatttaa attttaggac
    51421 tacactaagt aagtaaattt tattttaaag gagaatttgt tatgaattta acatttttag
    51481 gcttatgtat tgcctgtatg ggcgtatctg tcggtgaagg tttattgatg aatggactgt
    51541 ttaaatcagt agcacgccaa ccagatatgc tttctgagtt tcgtagtttg atgtttttag
    51601 gtgttgcctt tattgaagga actttctttg taactcttgt cttctcattt attatcaaat
    51661 aaatacatgg aacgagaaga aaagggagga ttttagatgg aagaaagtat taatccaatc
    51721 atctctattg gtcctgttat cttcaatctg actatgttag ccatgacttt gttgattgtg
    51781 ggagttattt ttgtctttat ttattgggca agccgcaata tgaccttgaa acccaaagga
    51841 aagcaaaatg tacttgagta tgtctatgac tttgttattg gatttacaga acctaacatt
    51901 ggttcgcgct acatgaaaga ttactcactc tttttccttt gtttattcct tttcatggtg
    51961 attgccaata accttggctt aatgacaaag cttcaaacga tcgatgggac taactggtgg
    52021 agttcgccaa ccgctaattt accgtatgac ttaaccttat cttttcttgt cattttgttg
    52081 acacatatag aaagcgttcg tcgtcgtgga tttaaaaaaa gtataaaatc ttttatgagt
    52141 cctgtttttg tcataccgat gaatatcttg gaagaattta caaacttctt atctttggct
    52201 ttgcggattt ttgggaatat ctttgcagga gaggtcatga cgagtttgtt acttcttctt
    52261 tcccaccaag ctatttattg gtatccagta gcctttggag ctaatttggc ttggactgca
    52321 ttttctgtct ttatttcctg catccaagct tatgttttta ctcttttgac atctgtgtat
    52381 ttagggaata agattaatat tgaagaggaa tagaaaggag taactgatgc acgtaacagt
    52441 aggtgaatta attggtaatt ttattttaat cactggctct tttattcttt tgctagtctt
    52501 gattaaaaaa tttgcatggt ctaatattac aggcattttc gaagaaagag ctgaaaaaat
    52561 tgcttcagat attgacagag ctgaagaagc ccgtcaaaaa gcagaagtat tggctcaaaa
    52621 acgcgaagat gaattggctg gtagccgtaa agaagctaag acaatcattg aaaatgcaaa
    52681 ggaaacagct gagcaaagta aggctaatat cttagcagat gctaaactag aagcaggaca
    52741 cttaaaagaa aaagccaatc aagaaattgc tcaaaataaa gtagaagctt tacagagtgt
    52801 taagggtgag gtcgcagatt tgaccatcag cttagctggt aaaatcatct cacaaaacct
    52861 tgacagtcat gcccataaag cactcattga tcagtatatc gatcagctag gagaagctta
    52921 atggacaaga aaacagtaaa ggtaattgaa aaatacagca tgccttttgt ccaattggta
    52981 cttgaaaaag gagaagaaga ccgtatcttt tcagacttga ctcaaatcaa gcaagttgtt
    53041 gaaaaaacag gtctgccttc ttttttaaaa caagtggcag tagacgagtc ggataaggaa
    53101 aaaacaattg cttttttcca agattctgtg tcacctttat tacaaaactt tatccaggtt
    53161 ctggcctaca atcacagagc aaatcttttt tatgatgtgc ttgtagattg cttgaaccga
    53221 cttgaaaaag aaacaaatcg atttgaagtg acgattacgt ctgctcatcc tctaactgat
    53281 gaacagaaga ctcgtttgct acctttgatt gagaaaaaaa tgtctctgaa agtaaggagt
    53341 gtaaaagaac aaatcgatga aagtctcatt ggtggttttg tcatttttgc caatcacaag
    53401 acaattgatg tgagtattaa acaacaactt aaagttgtta aagaaaattt gaaatagaaa
    53461 gtggtgttct tttggcaatt aacgcacaag aaatcagcgc tttaattaag caacaaattg
    53521 aaaatttcaa acccaatttt gatgtgactg aaacaggtgt tgtaacctat atcggggacg
    53581 gtatcgcgcg tgctcatggc cttgaaaatg tcatgagtgg agagttgttg aattttgaaa
    53641 acggctctta tggtatggct caaaacttgg agtcaacaga cgttggtatt atcatcctag
    53701 gtgactttac agatatccgt gaaggcgata caatccgccg tacagggaaa atcatggaag
    53761 tccctgtagg tgaaagtctg attggtcgtg ttgtggatcc gcttggtcgt ccagttgacg
    53821 gtcttggaga aatccacact gataaaactc gtccagtaga agcaccagct cctggtgtta
    53881 tgcaacgtaa gtctgtttca gaaccattgc aaactggttt gaaagctatt gacgcccttg
    53941 taccgattgg tcgtggtcaa cgtgagttga ttatcggtga ccgtcagaca gggaaaacaa
    54001 ccattgcgat tgatacaatc ttgaaccaaa aagatcaaga tatgatctgt atctacgtcg
    54061 cgattggaca aaaagaatca acagttcgta cgcaagtaga aacacttcgt cagtacggtg
    54121 ccttggacta cacaatcgtt gtgacagcct ctgcttcaca accatctcca ttgctcttcc
    54181 tagctcctta tactggggtt gctatggcgg aagaatttat gtatcaaggt aagcatgttt
    54241 tgattgtata cgatgatcta tcaaaacaag cggtagctta tcgtgaacta tcgctcttgc
    54301 ttcgtcgtcc tccaggtcgt gaagccttcc caggggatgt tttctatctc cacagccgtt
    54361 tgcttgagcg ctcagctaaa gtttctgatg aacttggtgg tggatcaatt acagccctac
    54421 catttatcga gacacaagca ggagatatct cagcctatat cgcaaccaac gtgatttcta
    54481 tcactgatgg acaaatcttc cttggcgatg gcctcttcaa tgcaggtatt cgtccagcca
    54541 tcgatgcggg ttcatctgta tctcgtgtag gtggttctgc acaaatcaaa gccatgaaga
    54601 aggttgctgg tacacttcgt atcgaccttg cttcataccg tgagttggaa gcctttacta
    54661 agtttggttc tgacttggac gcagcaacac aggctaagtt gaaccgtgga cgtcgtaccg
    54721 ttgaggtctt gaaacaacct gttcacaaac cattacctgt tgagaaacaa gtaaccattc
    54781 tttatgcttt gacacatggt ttcttggata ctgttccagt agatgatatt gttcgtttcg
    54841 aggaagagtt ccatgccttc tttgatgctc aacatccaga gattttggaa accattcgtg
    54901 atacaaaaga cttgccagaa gaagcagtct tggatgctgc gattacagag tttctcaatc
    54961 aatctagctt ccaataagaa tagaggtgtc agatggcagt gtctctaaat gatattaaaa
    55021 caaaaatcgc ctcaacaaaa aatacgagtc aaatcactaa tgccatgcaa atggtatcgg
    55081 ctgctaagtt aggtcgctct gaagaagctg ctcgcaactt ccaagtttac gctcagaaag
    55141 tgcgtaaact tttgacagat atccttcatg gtaatggagc tggtgcttca actaatccga
    55201 tgttgattag ccgttctgtg aagaagacag gctatatcgt tatcacttca gaccgcggtt
    55261 tggttggagg ttataattcc tctattttga aagctgttat ggagttgaaa gaagaatacc
    55321 acccagacgg taaaggtttt gaaatgatct gtatcggtgg gatgggagct gatttcttta
    55381 aggctcgcgg tattcaacca ctttatgaat tacgtggctt ggcagaccaa cctagctttg
    55441 atcaagttcg taagattatt tcaaaaactg ttgaaatgta ccaaaatgaa ctctttgatg
    55501 agctttatgt ttgctacaac caccatgtca atacgctaac cagtcaaatg cgtgtggaac
    55561 aaatgcttcc gattgttgac ttggatccaa atgaagcgga tgaagagtac agcttgactt
    55621 ttgaattgga aaccagccga gaagaaattc tggagcagtt gttgcctcag tttgcagaaa
    55681 gtatgattta cggtgccatt atcgatgcca agacagctga gaatgctgcg ggtatgacag
    55741 ccatgcaaac agcgacagat aatgctaaga aagtcatcaa tgatttgaca attcagtata
    55801 accgtgccag acaggcggcg attacacaag aaattacaga aatcgtagca ggtgctagtg
    55861 ccttagaata ggctctagtc cagctcgtat gaaaatgaac ttaggaccta gttgagctag
    55921 gaaccgacag tatcttatat agaataggag aaggagatga gttcaggtaa aattgctcag
    55981 gttatcggtc ccgttgtaga cgttttgttt gcagcagggg aaaaacttcc tgagattaac
    56041 aatgcacttg tcgtctacaa aaatgacgaa agaaaaacaa aaatcgtcct tgaagtagcc
    56101 ttggagttag gagatggtat ggttcgtact atcgccatgg aatcaacaga tgggttgact
    56161 cgtggaatgg aagtattgga cacaggtcgt ccaatctctg taccagtagg taaagaaact
    56221 ttgggacgtg tcttcaacgt tttgggagat accattgatt tggaagctcc ttttacagaa
    56281 gacgcagagc gtcagccaat tcataaaaaa gctccaactt ttgatgagtt gtctacctct
    56341 tctgaaatcc ttgaaacagg gatcaaggtt attgaccttc ttgcccctta ccttaaaggt
    56401 ggtaaagttg gacttttcgg tggtgccgga gttggtaaaa ccgtcttaat ccaagaattg
    56461 attcacaaca ttgcccaaga gcacggtggt atttcagtat ttactggtgt tggggaacgt
    56521 actcgtgagg ggaatgacct ttactgggaa atgaaagaat caggcgttat cgagaaaaca
    56581 gccatggtct ttggtcagat gaatgagcca ccaggagcac gtatgcgtgt tgcccttact
    56641 ggtttgacaa tcgctgaata cttccgtgat gtggaaggcc aagacgtgct tctctttatc
    56701 gataatatct tccgtttcac tcaggctggt tcagaagtat ctgccctttt gggtcgtatg
    56761 ccatcagccg ttggttacca accaacactt gctacggaaa tgggtcaatt gcaagaacgt
    56821 atcacatcaa ccaagaaggg ttctgtaacc tctatccagg ctatctatgt gccagcggat
    56881 gactatactg acccagcgcc agcaacagcc ttcgctcact tggattcaac aacaaacttg
    56941 gaacgtaaat tggtacaatt gggtatctac ccagccgttg acccacttgc ttcaagctca
    57001 cgtgccttgg cacctgaaat cgttggagaa gagcactatg cagttgctgc tgaagtaaaa
    57061 cgtgtccttc aacgttacca tgaattgcaa gatatcattg ctatccttgg tatggatgag
    57121 ctttctgatg aagaaaagac cttggttgct cgcgcccgtc gtatccagtt cttcttgtca
    57181 caaaacttca acgttgcgga acaatttact ggtcagccag gttcttatgt tccagttgct
    57241 gaaactgtac gtggctttaa ggaaatcctt gatggtaaat acgaccactt gccagaagat
    57301 gccttccgtg gtgtaggttc tatcgaagat gtgattgcaa aagctgaaaa aatgggattt
    57361 taagaggtga cctatggctc agttaactgt ccagatcgtg acaccagatg gtctcgtcta
    57421 tgatcaccat gccagctatg tatcggttcg aactctggat ggtgagatgg ggatcttgcc
    57481 acgacatgaa aatatgattg cggttttagc agttgatgaa gtaaaggtaa aacgtatcga
    57541 tgataaagat cacgtgaact ggattgcagt aaacggtggc gttattgaaa ttgccaatga
    57601 tatgatcaca atcgtcgctg actctgcaga acgtgctcgt gatatcgata ttagccgtgc
    57661 agaacgtgcc aaacttcgtg cagaacgtgc aattgaagaa gcacaagaca aacatttgat
    57721 tgaccaagaa cgtcgtgcta agattgcttt gcaacgtgcc attaaccgta ttaatgtcgg
    57781 aaatagacta taagaaaaaa tgaacttgaa aataccaagt tcatttttta ggctctttgt
    57841 caactgtagt gggttgaaaa aaagctaagc tcgagaaagg acaaattttg tcctttcttt
    57901 tttgatattc agagcgataa aaatccgttt tttgaagttt tcaaagttcc gaaaaccaaa
    57961 ggcattgcgc ttgataagtt tgatgagatt attggtcgct tccaatttgg cgtttgaata
    58021 gggtagttga agggcgttga cgattttctc tttgtccttt agaaaggttt taaagacagt
    58081 ctgaaaaaga ggatgaacct gcttaagatt gtcctcaata agtccgaaaa atttctctgg
    58141 ttccttattc tgaaagtgaa acagcaagag ttgatagagc tgatagtgat gtttcaagtc
    58201 ttgtgaatag ctcaaaagct tgtctaaaat ctctttattg gttaaatgca tacgaaaagt
    58261 agggcgataa aaatgtttat cgctgagttt acgactatcc tgttgtatga gcttccagta
    58321 gcgcttgata gccttgtatt catgagactt tcgatccaat tgattcatga tttgaacacg
    58381 cacacgactc atagcacggc taagatgttg tacaatgtga aagcgatcca acacgatttt
    58441 agcattcggg agtgaaacag tctgggagac tgtttcagcc tgagcctaga aatttgaaag
    58501 cgaagctgtt tagccaagtc atagtaagga ctaaacatat ccatagtaat aattttgacg
    58561 cgacatcgga caactctatc gtagcgaaga aagtgatttc gaatgatagc ttgtgttcta
    58621 ccctcaagaa cagtgataat atcgagcttt tcaaaatctt gcgcaatgaa gctcatcttt
    58681 ccctttgtaa aagcatactc atcccaagac ataatctcag gaagacgcga aaaatcatgt
    58741 ttaaagtgaa aatcattgag cttacgaata acagttgaag ttgagatgga aagctgatgg
    58801 gcaatatcag tcatagaaat cttttcaatt aacttttgag caatcttttg gttgatgata
    58861 cgagggattt ggtgattctt cttgacgata gaagtttcag cgaccatcat ttttgaacag
    58921 tgatagcact tgaatcgacg ctttctaagg agaattctag taggcatacc agtcgtttca
    58981 agataaggaa ttttagaagg tttttgaaag tcatatttct tcatttgact tccgcactca
    59041 gggcaagatg gggcgtcgta gtccagtttg gcgatgattt ccttgtgtgt atccttattg
    59101 atgatgtcta aaatctggat attagggtct ttaatgtcta gtaattttgt gataaaatgt
    59161 aattgttcca tatgattctt tctaatgagt tgttttgtcg cttttcatta taggtcatat
    59221 gggacttttt ttctacacaa aataggctcc ataatatcca taggggattt acccactatg
    59281 gatattatgg agccatttta caacagtgcc tcaaattcag atactgacat acctaattgc
    59341 tggctggcaa cctcagaagt cagaagacct tggcgtacta tgtctaggaa ggcaaaaagt
    59401 ttcctttctt cccttccttc aactttccca cgctctagtc cttgttcaag gccttcagcc
    59461 ctagcagttt ccaagggata gtcctgtgct aacaagactt gttcttctcg catatgtagt
    59521 tgactaaaca ttttcctgtc ctcctcggac cagctcttgt agtccagcag ttggtctgct
    59581 tggctgatgg ctcgctcagg ttgctgggta aagggcttgt tgccgaaaaa ctccaaccat
    59641 ggcttgcgaa ccttgtcttt gctggtttct ctgtattttt taattccaag aatgccatct
    59701 taaccagatg gttttcctgt ccattgttgg taatcgccaa tacctcacct gttgtgtctt
    59761 cgcgcatact aaagctatga aaagccaggt catctgagaa ataattacta tccacgatag
    59821 caatggcgta aacaggagcg atgtgtttgt agctctagtg agtatcacct tctcgctgac
    59881 gaattttttc aagattttga ttaacctgac tgcacaggta agcccacaag tgattgatga
    59941 aaaaattctg atgatggact tgaatctcaa taattacttg agtaccgtta tccaactccg
    60001 ccaagacgtc tatactggta taaaaatcct gcaccgagta aggcatggag agtaatacgt
    60061 gaatatcgct tccctccaaa atggtcacat tttttgctgg caagtccagc atatcgcgaa
    60121 taaattgaca agtgatttct ggattgctga atattttctt agcaatcaaa tcattagttg
    60181 ggctgatgcc cggatgtctg agaatcctcc tattcctcct tctatagtga gttgaaataa
    60241 gatgtgaata actctatcag gaaagtcaaa ttaatttata gaaatatttt agcagtcaag
    60301 gtgtactatt atagattcaa tatactatgt tatagcacat ttttaaatct aggtttacta
    60361 aattctctgg ctctatctat ttattcggaa aaagtttgaa aaatacttgc tctagctctt
    60421 cccaatggta ttttttgatt ctttccttta taatgggtgt atggataaga aaaaattatt
    60481 attgattgat gggtcttctg tagcttttcg ggcgtttttt gcgctgtatc agcagttgga
    60541 ccgttttaag aatgcggctg gtttgcatac caatgcgatt tatggtttcc agttgatgtt
    60601 gagtcatttg ttggagcggg ttgagccgag tcatattttg gtggcttttg atgcgggaaa
    60661 gacgaccttc cggacagaga tgtatgcgga ctataagggt ggtcgggcca agactcctga
    60721 tgagtttcgt gagcaatttc ctttcattcg tgagttgctg gatcatatgg ggattcgtca
    60781 ctatgagctg gctcagtatg aggcggatga catcattggg acgctggata agctagcaga
    60841 gcaggatggt tttgatatta ccattgtcag tggggacaag gatttgattc agctgacgga
    60901 tgagcatacg gtggttgaaa tttccaagaa aggtgtggct gagtttgagg cctttacgcc
    60961 agattacctc atggaagaaa tgggcctcac accagctcag tttatcgatc tcaaggcgct
    61021 catgggtgat aagtcggata atatccctgg ggtgaccaaa gtcggtgaaa agacgggtat
    61081 taagctcttg ctggagcatg gttcgcttga ggggatttat gaaaatattg atggaatgaa
    61141 gacttctaag atgaaggaaa atctcatcaa tgacaaggaa caggcctttt tgtctaaaac
    61201 actagcgacc attgatacca aggcaccgat tgcgattggt ttagaggact tggtctatag
    61261 tggtccagat gttgaaaatc ttgggaaatt ctacgatgag atgggcttca aacagctcaa
    61321 gcaggcttta aatgtgtcgt cagctgatgt ggctgagggt ttggatttta ctattgttga
    61381 ccaaatcagt caagatatgc tgagtgaaga gtctatcttc cactttgagc tttttggtga
    61441 gaattaccat acggataatt tggttggatt tgcctggtct tgtggggata agctctatgc
    61501 cacagacaag cttgagctgt tgcaagaccc gattttcaag gatttcttag aaaaaacatc
    61561 tctgagagtt tatgacttta agaaggttaa agttcttttg caacgttttg gtgtggattt
    61621 gcaggcgcct gcttttgaca tccgtttggc taaatacctc ctttcgactg tggaggacaa
    61681 tgaaattgcg accatcgcta gtctttatgg tcagacttac ttggttgatg atgaaacttt
    61741 ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag aaattcttgg aacacttagc
    61801 ttgtaaactt gctgttttgg tagaaacaga gcctatttta cttgaaaaac tcagcgaaaa
    61861 tgggcaatta gagcttcttt atgatatgga gcaacctctg gcttttgttc ttgccaagat
    61921 ggaaattgct gggattgtgg tcaagaaaga gaccttgctt gagatgcagg ctgaaaatga
    61981 gcttgtcatt gaaaaactga ctcaagagat ttacgagctg gctggtgagg agtttaatgt
    62041 caactcgcct aagcagttgg gcgtgcttct ctttgagaaa ttgggacttc ctctagaata
    62101 cactaagaaa accaagacag gttattcgac agcagtggat gtgttggagc gtctcgctcc
    62161 tattgctccg attgttaaga aaatcctgga ttaccgtcaa attgctaaga ttcaatctac
    62221 ttatgtaatt ggcttgcagg actggatttt ggctgatgga aagattcata ctcgctatat
    62281 gcaggatttg acccagaccg ggcgtttgtc tagtgtggat ccaaacttgc aaaatattcc
    62341 tgcccgattg gaacaagggc gcttgattcg gaaggctttt gtgccagagt gggaggatag
    62401 tgtgctactc agctctgact attcacagat tgaattgcgc gttttggcgc atatttctaa
    62461 ggatgagcac ttgactaagg ccttccaaga gggggcagat atccatactt cgacagccat
    62521 gcgggtcttt ggcattgagc gtcctgataa tgtgactgca aatgaccgtc gcaatgccaa
    62581 ggcagttaac tttggagtgg tttatgggat ttcagacttt ggcttgtcta ataatttggg
    62641 aattagtcgt aaggaagcca aagcctacat tgatacctac tttgaacgtt ttccaggtat
    62701 taaaaactac atggatgaag tggtgcggga ggcgcgtgat aagagctatg tagagaccct
    62761 ctttaagcgt cgccgtgagt tgccagatat caattcgcgc aacttcaata ttcgtggttt
    62821 tgcggagcga actgctatca actcacctat ccagggttcg gcagcagata ttctcaagat
    62881 tgccatgatt cagctggata aagccttggt tgcaggtggt tatcagacca agatgctgtt
    62941 acaagtgcac gatgaaatcg tccttgaagt gcctaaatct gaattggtag agatgaaaaa
    63001 attggtgaaa caaaccatgg aagaagccat tcaactcagt gttcctctta tcgcagatga
    63061 gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg ggctagtcct ccttttttgt
    63121 agtagaattc tgcaaacctt ttcaaaatgt gctatactga tgaaaaagga ggatttctat
    63181 gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt cagtatctcg caacgagtaa
    63241 aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca accagtaatc gagtaacaaa
    63301 ggaaatgcag gctcggggct ataaaatcat cccagtcaat cccaagacgg caggtggcga
    63361 aatcttgggt gaaaaggctt atgctagcct cgctgaaatt ccttttcctg tagatattgt
    63421 caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg cgtgattttc tcaaagctga
    63481 tgccaagatt ttttgggcac agctaggact tgaaagttta gaagcgaaag agattttgcg
    63541 tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc aagagagaac atactcgctt
    63601 gattgaggaa gcataagaaa aaggtagctg atgggctacc ttttgtgtta tactcaatga
    63661 aaatcaaaga gcaaactagg aaactagctg cgggttgctc aaaacactgt tttgaggttg
    63721 cagatagaac tgacgaagtc agctcaaaac accgttttga ggttgtagat agaactgacg
    63781 aagtcagctc aaaacaccgt tttgaggttg tagatagaac tgacgaagtc agtaacatat
    63841 atacggcaag gcgacgttga cgtggtttga agagattttc gaagagtatt agaaaatgcc
    63901 gataagggtc tgcataccaa ggctggtgag gatgatggca atccagcaga cggctccgag
    63961 aacaatggat tttccactag atttgaccat agcgaccaga ttagttttga gaccgatggc
    64021 actcatggcc atgataatga ggaatttaga gagttgtttg agaggggtaa agaaactact
    64081 agacacaccg agagaggtca gaagggtggt taggagcgat gcaaggatga agtaaaggat
    64141 aaaaagtggg aagacttttt tcagttgtaa gccttgctta tttttttgct cgcgactttg
    64201 ccagtaggag agaaagagag tgatggggat gatagctagg gtgcgcgtga gtttgacaat
    64261 ggttgcagat tcgagggtat tggtctggta gagactgtcc caagcgctag ctgtggcggt
    64321 tacagaggaa gtatcattga ccgcagttcc tgcaaagagg gcgaagccgt cattggatag
    64381 atgaagccag gtaccgaggg ttggaaagat gagcgcagcc aagacattga agaaaaagat
    64441 aacggagatg gcttgggcta cttccttttc cttagcatgg ataacaggcg ctgtcgctgc
    64501 aatggcagaa cccccacaga tagaagaacc tactccaatc aaggtagcca gttttgtatc
    64561 cagggcaaag aagcgctgga agaggtaggc aacaatcaag gctattgaaa tggtggaaag
    64621 gatgacaggg agtgaagatt gcccaactgc gaagacttga gagatattga gaccaaaacc
    64681 aagtaagata acggcatact ggagcaattt cttggaacta aaggtcaatc cagcatccag
    64741 ttgtttatga gacgagagaa agggatgtag gagcatgccc ataaaaatcg caaaaacggg
    64801 tgcgccaatc acagggaaga atcctcctaa gtaccaagat aggatggaaa tgagaaggca
    64861 gaccaagatg cctgctccat tttttgatag aaatgacata aaaacctcca aatagaatct
    64921 gttaccatta tagacctgta aacaggaaaa gtaaaataga aagtggaaag ctattctcta
    64981 atgtattttt gcggtcgggg ggcttttgta gtataataga gatacgtttt gaaagtagga
    65041 ggtatctatg gacttaacta agcgctttaa taaacagtta gataaaattc aagtttcgtt
    65101 gattcgtcag tttgaccagg ctatttcgga gattcctggg gtcttgcgtt tgaccttggg
    65161 ggaacctgat tttacaacgc cagaccatgt caaggaggcg gccaagcgag cgattgatca
    65221 gaaccaatcc tactatacag ggatgagtgg tctgctgact ctacgtcagg cagccagtga
    65281 ctttgttaag gaaaagtacc aactggacta tgctcctgaa aatgaaatct tggttacaat
    65341 tggggcgaca gaggctttat ctgcgacttt gacggctatt ttggaagagg gcgacaaggt
    65401 acttttgcca gctcctgctt atccaggcta tgaaccgatt gttaacttag ttggggcaga
    65461 aattgttgag attgatacga ctgaaaatgg ttttgtcttg actcctgaga tgttggagaa
    65521 ggccattttg gagcagggtg ataagctcaa ggcggttatt ctcaactatc cagccaatcc
    65581 gacaggaatt acctacagtc gagagcagtt agaggccttg gcagctgttt tacgcaagta
    65641 cgaaattttt gttgtctgtg atgaggttta ctcagaattg acctacacag gcgaagccca
    65701 tgtgtctcta ggaacgatgt tgagagacca ggctattatt atcaatggtt tgtctaaatc
    65761 gcatgccatg acaggttggc gtttggggct gattttcgct cctgcggcct tcacagccca
    65821 gttaatcaag agtcaccaat acttggtcac tgccgcaaat accatggcgc aacatgctgc
    65881 ggtagaagcc ttgacggctg gtaaaaatga tgcggagccc atgaagaagg aatatatcca
    65941 gcgtcgggac tatatcatcg aaaaaatgac tgctcttggt tttgagatta tcaaaccaga
    66001 cggtgccttc tatatttttg ctaaaattcc agcgggctac aatcaagact cctttgcttt
    66061 tctgaaggat tttgctcaga agaaggccgt tgcctttatc cctggtgcag cctttggacg
    66121 ttacggggaa ggctacgtcc gcctatctta tgcagccagc atggagacta tcaaagaagc
    66181 catgaaacga cttgaggagt acatgagaga agcatgattc agtctatcac gagtcaaggc
    66241 ttggtgcttt acaatcgcaa ttttcgtgag gatgacaagc tcgtcaaaat ttttacagag
    66301 caggttggca aacgcatgtt ttttgtcaaa cacgctggtc agtctaagct ggcgcctgtt
    66361 attcagccct tggtgctggc acgatttcta ttgcgaatca atgatgacgg actcagttac
    66421 atcgaagact atcatgaggt catgactttt cccaagatta atagtgacct ctttgtcatg
    66481 gcctatgcga cctatgtggc agctcttgca gatgctagtt tgcaggacaa tcagcaggat
    66541 gctcccttgt ttgctttttt gcaaaagact ttggagttga tggaagcagg cttggattat
    66601 caggttttga ccaatatttt tgaaattcaa attttgactc gatttggaat cagcctcaat
    66661 tttaatgagt gtgtcttctg ccatcgggtt ggtcaggctt ttgacttttc tttcaaatat
    66721 ggagcctgcc tctgtccaga gcattatcat gaggataaga gacgttgtca tctcaatccc
    66781 aatatcccct atctgctcaa tcaatttcaa gctattgatt ttgagacttt ggagaccatt
    66841 tcgctcaagc ctggaatcaa gcaagagcta cgccaattta tggatcaact atatgaagag
    66901 tacgttggga ttcacctaaa atcaaagaaa tttattgatt ccctagcaga ctggggacaa
    66961 ttactaaaag aggaaaagaa atgaaaaaaa tcgcagtaga tgccatgggg ggcgattacg
    67021 cacctcaggc cattgttgag ggtgtcaatc aagccctatc tgacttttca gatatcgagg
    67081 ttcaacttta cggagatgaa gctaaaatca agcaatatct gacagcgaca gagcgcgtca
    67141 gcattatcca tacggatgag aagattgatt cggatgatga acctacgaga gctattagga
    67201 ataagaaaaa tgccagtatg gtattggcag ccaaggctgt caaagatggt gaagcagacg
    67261 ctgtcctttc ggctgggaat acaggtgcct tgttggctgc tggattcttc atcgtgggtc
    67321 gtatcaagaa tatcgaccgt cctgggctta tgtcgacttt gccgactgta gatgggaagg
    67381 gatttgacat gctagacctc ggtgccaatg cggaaaatac agcccagcac ctccatcaat
    67441 acgcggttct aggatccttc tatgctaaaa atgttcgtgg cattgcgcaa ccacgtgttg
    67501 gcttgctcaa taacggaaca gagagtagca agggcgatcc gcttcgtaag gaaacttatg
    67561 aattactagt ggctgatgaa agtttgaact ttatcggaaa tgtggaagcg cgtgatttga
    67621 tgaatggtgt tgcagatgtt gttgtggcag atggtttcac gggaaacgct gtgctcaaat
    67681 ccatcgaagg gacagctatg ggaatcatgg gcttgctcaa gacagctatt acaggtggtg
    67741 gtcttcgagc gaaactaggt gccctccttc tcaaggacag cctcagaggt ttgaaaaaac
    67801 agctcaatta ttcagatgtt ggtggagcgg tcttgtttgg tgttaaggca cctgttgtca
    67861 agactcatgg ctcaagcgat gccaaggctg tttatagtac gattcgccag attcgtacca
    67921 tgctagaaac agacgtagtt gcccagactg cgcgtgaatt ttcaggagaa taaaagagat
    67981 gacagaaaaa gaaatttttg accgtattgt gaccattatc caagagcgac agggagagga
    68041 ctttgtcgtg acagaatcct tgagtctgaa agacgatttg gatgcggatt ctgttgactt
    68101 gatggagttt atcttgactc tggaagatga atttagtatc gaaatcagcg atgaagaaat
    68161 tgaccaactc caaaacgtag gagatgtggt taaaatcatt caaggaaaat agcaatcgga
    68221 gttccaagtc aacggaagta gatggttttt agaaatgaga aatatcggac aagctggtaa
    68281 aatcttggct gacagtggtt atcaagggct catgaagata tatcctcaag cacaaactcc
    68341 gaggaaatca agtaaactta agccattaac tcttgaagat aaagcctgta atcatgcgct
    68401 atctaaggag agaagcaagg ttgagaatat ct
//
LOCUS       DAVMAF010000002        64730 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMAF010000002 DAVMAF010000000
VERSION     DAVMAF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.002"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..612
                     /locus_tag="VYO97_000075"
     CDS             <1..612
                     /locus_tag="VYO97_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1752968.1"
                     /translation="YRTYEEIAADFGVHESNLIRRSQWVEVTLVQSGVTISRTPLSSE
                     DTVMIDATEVKINRPKKQLANDSGKKKFHVMKAQAIVTSQGRIVSLDITVNYCHDMKL
                     FKMSRRNIGQAGKILTDSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHALSKERS
                     KVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(1350..1610)
                     /locus_tag="VYO97_000076"
     CDS             complement(1350..1610)
                     /locus_tag="VYO97_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752969.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(1669..2220)
                     /locus_tag="VYO97_000077"
     CDS             complement(1669..2220)
                     /locus_tag="VYO97_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752970.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATFIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAP"
     gene            complement(2210..2407)
                     /locus_tag="VYO97_000078"
     CDS             complement(2210..2407)
                     /locus_tag="VYO97_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752971.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(2411..3205)
                     /locus_tag="VYO97_000079"
     CDS             complement(2411..3205)
                     /locus_tag="VYO97_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752972.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(3613..4038)
                     /locus_tag="VYO97_000080"
     CDS             complement(3613..4038)
                     /locus_tag="VYO97_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752973.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            complement(4167..4550)
                     /locus_tag="VYO97_000081"
     CDS             complement(4167..4550)
                     /locus_tag="VYO97_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752974.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKETVLG"
     gene            complement(4572..4778)
                     /locus_tag="VYO97_000082"
     CDS             complement(4572..4778)
                     /locus_tag="VYO97_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752975.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(4765..4926)
                     /locus_tag="VYO97_000083"
     CDS             complement(4765..4926)
                     /locus_tag="VYO97_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1752976.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(5285..6241)
                     /gene="lytA"
                     /locus_tag="VYO97_000084"
     CDS             complement(5285..6241)
                     /gene="lytA"
                     /locus_tag="VYO97_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1752977.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6617..7987)
                     /locus_tag="VYO97_000085"
     CDS             complement(6617..7987)
                     /locus_tag="VYO97_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1752978.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(8294..9460)
                     /gene="recA"
                     /locus_tag="VYO97_000086"
     CDS             complement(8294..9460)
                     /gene="recA"
                     /locus_tag="VYO97_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1752979.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(9515..10771)
                     /locus_tag="VYO97_000087"
     CDS             complement(9515..10771)
                     /locus_tag="VYO97_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1752980.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(10855..11871)
                     /locus_tag="VYO97_000088"
     CDS             complement(10855..11871)
                     /locus_tag="VYO97_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1752981.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(11879..12397)
                     /locus_tag="VYO97_000089"
     CDS             complement(11879..12397)
                     /locus_tag="VYO97_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1752982.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(12387..12830)
                     /gene="tsaE"
                     /locus_tag="VYO97_000090"
     CDS             complement(12387..12830)
                     /gene="tsaE"
                     /locus_tag="VYO97_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1752983.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(12916..13536)
                     /locus_tag="VYO97_000091"
     CDS             complement(12916..13536)
                     /locus_tag="VYO97_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1752984.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(13919..14782)
                     /locus_tag="VYO97_000092"
     CDS             complement(13919..14782)
                     /locus_tag="VYO97_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1752985.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            15591..17606
                     /locus_tag="VYO97_000093"
     CDS             15591..17606
                     /locus_tag="VYO97_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1752986.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNIGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            17608..18240
                     /locus_tag="VYO97_000094"
     CDS             17608..18240
                     /locus_tag="VYO97_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1752987.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(19185..19598)
                     /gene="ndk"
                     /locus_tag="VYO97_000095"
     CDS             complement(19185..19598)
                     /gene="ndk"
                     /locus_tag="VYO97_000095"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1752988.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(19709..23386)
                     /gene="rpoC"
                     /locus_tag="VYO97_000096"
     CDS             complement(19709..23386)
                     /gene="rpoC"
                     /locus_tag="VYO97_000096"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1752989.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NKETTEVVVDTAVETVEETVE"
     gene            complement(23419..27030)
                     /gene="rpoB"
                     /locus_tag="VYO97_000097"
     CDS             complement(23419..27030)
                     /gene="rpoB"
                     /locus_tag="VYO97_000097"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1752990.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            27446..27516
                     /locus_tag="VYO97_000098"
     tRNA            27446..27516
                     /locus_tag="VYO97_000098"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27478..27480,aa:Cys,seq:gca)
     gene            complement(27714..29045)
                     /locus_tag="VYO97_000099"
     CDS             complement(27714..29045)
                     /locus_tag="VYO97_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1752991.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(29336..30160)
                     /gene="endA"
                     /locus_tag="VYO97_000100"
     CDS             complement(29336..30160)
                     /gene="endA"
                     /locus_tag="VYO97_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1752992.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(30199..30387)
                     /locus_tag="VYO97_000101"
     CDS             complement(30199..30387)
                     /locus_tag="VYO97_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1752993.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(30380..31663)
                     /gene="murA"
                     /locus_tag="VYO97_000102"
     CDS             complement(30380..31663)
                     /gene="murA"
                     /locus_tag="VYO97_000102"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1752994.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(31687..31917)
                     /locus_tag="VYO97_000103"
     CDS             complement(31687..31917)
                     /locus_tag="VYO97_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1752995.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(31975..33012)
                     /locus_tag="VYO97_000104"
     CDS             complement(31975..33012)
                     /locus_tag="VYO97_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1752996.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(32996..33484)
                     /gene="coaD"
                     /locus_tag="VYO97_000105"
     CDS             complement(32996..33484)
                     /gene="coaD"
                     /locus_tag="VYO97_000105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1752997.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(33474..34013)
                     /gene="rsmD"
                     /locus_tag="VYO97_000106"
     CDS             complement(33474..34013)
                     /gene="rsmD"
                     /locus_tag="VYO97_000106"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1752998.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(34078..35070)
                     /locus_tag="VYO97_000107"
     CDS             complement(34078..35070)
                     /locus_tag="VYO97_000107"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1752999.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(35181..35864)
                     /locus_tag="VYO97_000108"
     CDS             complement(35181..35864)
                     /locus_tag="VYO97_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1753000.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFSELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(35920..36660)
                     /locus_tag="VYO97_000109"
     CDS             complement(35920..36660)
                     /locus_tag="VYO97_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1753001.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            36694..36972
                     /locus_tag="VYO97_000110"
     CDS             36694..36972
                     /locus_tag="VYO97_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1753002.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            37050..37976
                     /gene="yidC"
                     /locus_tag="VYO97_000111"
     CDS             37050..37976
                     /gene="yidC"
                     /locus_tag="VYO97_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1753003.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(38051..38845)
                     /gene="pflA"
                     /locus_tag="VYO97_000112"
     CDS             complement(38051..38845)
                     /gene="pflA"
                     /locus_tag="VYO97_000112"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1753004.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(38971..40221)
                     /locus_tag="VYO97_000113"
     CDS             complement(38971..40221)
                     /locus_tag="VYO97_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1753005.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(40288..41115)
                     /gene="purR"
                     /locus_tag="VYO97_000114"
     CDS             complement(40288..41115)
                     /gene="purR"
                     /locus_tag="VYO97_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1753006.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(41382..42323)
                     /locus_tag="VYO97_000115"
     CDS             complement(41382..42323)
                     /locus_tag="VYO97_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1753007.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(42313..43569)
                     /locus_tag="VYO97_000116"
     CDS             complement(42313..43569)
                     /locus_tag="VYO97_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1753008.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            complement(43571..44233)
                     /locus_tag="VYO97_000117"
     CDS             complement(43571..44233)
                     /locus_tag="VYO97_000117"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1753009.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(44196..44852)
                     /locus_tag="VYO97_000118"
     CDS             complement(44196..44852)
                     /locus_tag="VYO97_000118"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1753010.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(44863..45741)
                     /gene="rsgA"
                     /locus_tag="VYO97_000119"
     CDS             complement(44863..45741)
                     /gene="rsgA"
                     /locus_tag="VYO97_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1753011.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(45743..46615)
                     /gene="rsmA"
                     /locus_tag="VYO97_000120"
     CDS             complement(45743..46615)
                     /gene="rsmA"
                     /locus_tag="VYO97_000120"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1753012.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQADLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(46746..47054)
                     /locus_tag="VYO97_000121"
     CDS             complement(46746..47054)
                     /locus_tag="VYO97_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753013.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(47047..47688)
                     /locus_tag="VYO97_000122"
     CDS             complement(47047..47688)
                     /locus_tag="VYO97_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753014.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(47690..49720)
                     /locus_tag="VYO97_000123"
     CDS             complement(47690..49720)
                     /locus_tag="VYO97_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1753015.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSIYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(49797..50093)
                     /locus_tag="VYO97_000124"
     CDS             complement(49797..50093)
                     /locus_tag="VYO97_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1753016.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(50413..51324)
                     /locus_tag="VYO97_000125"
     CDS             complement(50413..51324)
                     /locus_tag="VYO97_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753017.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYDKEEEYDKKEACLEEI
                     EDLFYEQLPNEEKVWFEATRATIDVIRSGQPEYGETVLDDYFKTIYDKELFLINELEV
                     INLYFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIAPEYLFALSNTLFSGLACL
                     DNLSSYDSLGAYIFSLNHIMEKTQDFQKKPIILMLEWKLSLIINNDYVSAEQFYQKSK
                     LFADIIENSYLVTMLEKQWQEDLKKYL"
     gene            complement(51396..51956)
                     /gene="rnmV"
                     /locus_tag="VYO97_000126"
     CDS             complement(51396..51956)
                     /gene="rnmV"
                     /locus_tag="VYO97_000126"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1753018.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(51956..52729)
                     /locus_tag="VYO97_000127"
     CDS             complement(51956..52729)
                     /locus_tag="VYO97_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1753019.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(53034..53687)
                     /locus_tag="VYO97_000128"
     CDS             complement(53034..53687)
                     /locus_tag="VYO97_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1753020.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKPETTPGGDKGGNTGNSD
                     ANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPKTT
                     SAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            complement(53950..54084)
                     /gene="rpmH"
                     /locus_tag="VYO97_000129"
     CDS             complement(53950..54084)
                     /gene="rpmH"
                     /locus_tag="VYO97_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1753021.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(54229..55443)
                     /locus_tag="VYO97_000130"
     CDS             complement(54229..55443)
                     /locus_tag="VYO97_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1753022.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(55599..55904)
                     /locus_tag="VYO97_000131"
     CDS             complement(55599..55904)
                     /locus_tag="VYO97_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753023.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPGFIG
                     FLVFHVLYHLIYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            56066..56518
                     /locus_tag="VYO97_000132"
     CDS             56066..56518
                     /locus_tag="VYO97_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1753024.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(56553..57941)
                     /locus_tag="VYO97_000133"
     CDS             complement(56553..57941)
                     /locus_tag="VYO97_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1753025.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            58007..58969
                     /locus_tag="VYO97_000134"
     CDS             58007..58969
                     /locus_tag="VYO97_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1753026.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            complement(58998..59291)
                     /locus_tag="VYO97_000135"
     CDS             complement(58998..59291)
                     /locus_tag="VYO97_000135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753027.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDRTDEVS
                     SKHCFEVADETDEVSSKHVFEVVDETDEVSSKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(59355..60365)
                     /gene="ccpA"
                     /locus_tag="VYO97_000136"
     CDS             complement(59355..60365)
                     /gene="ccpA"
                     /locus_tag="VYO97_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1753028.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(60790..61389)
                     /locus_tag="VYO97_000137"
     CDS             complement(60790..61389)
                     /locus_tag="VYO97_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753029.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQTKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(61391..62488)
                     /locus_tag="VYO97_000138"
     CDS             complement(61391..62488)
                     /locus_tag="VYO97_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1753030.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMCEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     DSMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(62488..63225)
                     /locus_tag="VYO97_000139"
     CDS             complement(62488..63225)
                     /locus_tag="VYO97_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753031.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGDINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(63227..64111)
                     /locus_tag="VYO97_000140"
     CDS             complement(63227..64111)
                     /locus_tag="VYO97_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753032.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(64098..64292)
                     /locus_tag="VYO97_000141"
     CDS             complement(64098..64292)
                     /locus_tag="VYO97_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753033.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(64289..64495)
                     /locus_tag="VYO97_000142"
     CDS             complement(64289..64495)
                     /locus_tag="VYO97_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753034.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        19017 a        14490 c        11901 g        19322 t
ORIGIN      
        1 tatcgaactt atgaagaaat tgcggctgat tttggtgttc acgaaagcaa cttaatccgt
       61 cggagccaat gggttgaagt aactcttgtt caaagtggtg ttacgatttc aagaactcct
      121 ctcagctctg aggacacggt aatgattgat gcgacggaag taaaaatcaa tcgccctaaa
      181 aaacaattag cgaatgattc tggtaaaaag aaatttcacg ttatgaaggc tcaagcgatt
      241 gtcacaagtc aagggagaat tgtttctttg gatatcactg tgaactattg tcatgatatg
      301 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt gactgacagt
      361 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
      421 ctcaagccgc taacagctga agataaagcc tgtaaccatg cgctatccaa ggagagaagc
      481 aaggttgaga acatctttgc caaagtagaa acgtttaaaa tgttttcaac aacctatcga
      541 aatcatcgta aacgctttgg attacgaatg aatttgattg ctggtattat caatcatgaa
      601 ctaggattct agttttgcag gaagtctatt atatatgcac agacatatga aatctttttg
      661 agacagcagt tatgatatat ttatccattc acaacatctt ctgtactaat actacaatat
      721 tggtacattt tgattacaca atcgtttcaa aaaatatgat ggaagcctga gtattttata
      781 gtaattccct tcctatcaac tagatcaacc agttgagtgt tgaatgttga aacaccctag
      841 cctcagcaaa ggactatgat cagcatttcc tagccaatag gacgctccaa atcacctatt
      901 ctaagtccat cactagcttg gtcagtaaga tacaggctgc aaggcaagac tgacgtggtt
      961 gatgagattt tggaagagta ttacaatcat agattcacca gtagacttga ccgcagattt
     1021 aactatagta gattgaaact agaatagtac acttctgctt ctaaaacatt gttagaaatc
     1081 gatttaactg tcctgatcaa tttgtcctgt tctcgtttca ttctaccata ctaaaaaacc
     1141 acctgaaaca aatcaagtga ctagaggata aaaacgaaga ctgggcaaaa agttttgaaa
     1201 agcaaaaaac gcataatatc aggtcttgaa aaaccttgat actatacgtt tgattgtggg
     1261 aagattgact tcattttctc ctgaaattga agttttttct agtttccaga tatagacgaa
     1321 ttcaatatag ccatctcgct acaattattt taaccatcga gaaaattttt cagaatttca
     1381 aaaatttcat tttgatccaa tgaaatgtca ttattttgaa gaatagttga cttcacaggc
     1441 tctaattcta tcacaaagcg gtgcatgtct gcttttaccg cactacttga tgttatagcc
     1501 gataactttt catctcctag caaggaagct gtcaaacggg taatgtcatt caaatgcttt
     1561 ttaatggact tacttaaacc ttgagcacct gtggcagagc gctccctcat atccagccaa
     1621 gctttagcct taaaaataat taatactcaa tgaaaatcaa agagcaaact agggagctag
     1681 ctgcaggctg ctcaaaacac tgttttgagg ttgtagatgg aagctgacgt ggtttgaaga
     1741 gattttcgaa gagtatagac cacaattact caataccgaa tacccctgaa tggtttcttt
     1801 tacatgcacc aatatattat aataatcttc atccaataat aaggctgata aactagcatc
     1861 ttggtcaaaa tataagggaa tttctcgacc gtccttcttt aatggatatt ctggtaagat
     1921 actaaatagt tcaatcatag aaggaaactc agaattagtt gttgtaaatc gaaaaagctg
     1981 cgctttctca tctttctgac ttccttgata ctctcccaat tctaaaaaat gattcaaggt
     2041 agtataaaat tccttatttt ttacttcatc aatgatgacc atatcataat cttttgttgt
     2101 gcgactttta aatccttgcg aatccaatac gataaaggta gcagttcccc caatcagaac
     2161 ataatagttc tgaaaatccg caaacatttc ttgaaaaata actttcgtat tagctggcat
     2221 catcttctcc cagatactgt aatatcatat tttctagtgc ttcactctct tcctctatac
     2281 gtgggtcatc atcatctttt aaggtcaaat aaagagaaat cggatctaca aattgtttat
     2341 catgattatt tttaaaatca ttccaaaact cagatacaaa aggacgatat ttccatatct
     2401 ctagcatctt tcattttaaa acatgctgag aaagtggcaa ggataactga ttgaattttc
     2461 tctgccatat gacatagcta atattttcat ccgtttcagc taaaaaagtt gaatgcgaca
     2521 aagcataagc accaccatat agaaggttag aaacagattt tatctgcttt atatcgccat
     2581 ctggcaataa aatccgtttt ttgatgggat taaataaaca tgacacggat tttaagaata
     2641 attctttctt tgacaccgta tatgtgtaaa gcttattttg cttgtttaac caatataagg
     2701 ctttaaaagt cctcaaacac ctataaattg ttgaatttgg aagtccagtg acttgtgaaa
     2761 gcaaatcaac atctactact ttttgacctt ttgtcaataa aaaggcaatc cacgttaatt
     2821 gttcgctagg tgttaattcc ttagggactt cagtatcatt cgcattgagt actagtccca
     2881 atggagggaa gaagaggttt cccttaaagt ctacaaacgg aactctagct tgaagtaatt
     2941 gctttttttc actgtctgat aacttcgaaa acaccaaaac aacatccata ttggcttttt
     3001 cacccatagt gcgagcctga gtaacaaatg aactcaaact cccccttctc ttttctttaa
     3061 tcaataaaaa tgattcctta tgaaaagtca tttgagtata ctcaaacctt tttacaaaac
     3121 tgattggtaa agtcaaagtc agttcctcaa tactgacatc aacataaaag gattgaaaaa
     3181 ttgacattat ctttttgaca ttcatgtttt ttccttattc tatcattttt attatatttt
     3241 atcattcggt ataataaaat ataagttttt tgataaaata accaaaaact actcgctatc
     3301 ccctctcaca ttaccctaca tatcgtttga catgcgactg atagtttagg aaaacttcaa
     3361 ggagaacttt tctctcatcc actatgcagg acttactatt tcacttctac tcctataggc
     3421 tcaatttgac acttttcttt ttcgaattgc tcatatgcct cctgaatagt gagttcgatt
     3481 tatccaaatt tatttttgtc tattttgatg aatacttcca cgaagatagt ctgaaaatga
     3541 atttgcacca attttccttc tctttttaag catattattc cagatatgat tgccctctta
     3601 ttcagtaata aattacgctt taaacgcttc aatcagtata tctcctgttg tcatatattc
     3661 taaagaaaat tcttctacat atgatttaac atctcttagg ttattacttc ctaatatccc
     3721 attatgcttt ttcgctaagg aaatagatgc cccttctccc ttaccaataa tcttgttact
     3781 atcatgattt cttgttaaat ctctatataa tgcgtattct tcagttccaa tgtctatgct
     3841 cacaatctca gctgaaccct tagctaccaa ctggtctatc ctagatttta aatggggaat
     3901 tgtaggtata ttgatttcat catacacctc ttgtggaata acaattttac ccaaatagag
     3961 cttttctaaa agatgttcag tgccaaccca taaaaatact gaaatacaat ctgcatcaat
     4021 aaatacacga ctagtcaaac gacaactccc ccctcttcat atagcccata tacaatatca
     4081 tatctgaaag catctagtaa cagttcctca tacttccctt gcgaaattct gttattttct
     4141 aaaagttgtt cagttgaatt aatatattat cctaataccg tttccttttt actttctgac
     4201 aaaggacgat ataaacttgt atcatagcct aatcttgaag ctgtctctat aacactaata
     4261 tccatatttt taatttcttc tgcatcaagg tatccatcat tcctcaatct atataacata
     4321 gctttatgac tgataccata aaactgaccc aattttataa tatcttctac ttcaagatga
     4381 gttctattgg cattttctct aatttcctca accatcctat acagtgaaga tgggaaaatt
     4441 aaaaaataag aagcaaactg atccgctttt ctttcagttt catctccttc accaatcaag
     4501 ataagactga ctgaactctt cttcacctca tcataataaa gatgatacag ttcatgtgct
     4561 aaagaaaatc tttaccttcc taatgacatg tctgaattga ctgcaatgag actgaactga
     4621 gttcctttat aacagactcc gctaatattc tttccgagtc cataaaatac cagcgtcaaa
     4681 ttttctatct tttgtaccaa tttaaaaata tctatcggcg attcaccatc agctcccaac
     4741 ttttttctaa gatttggagc tttattactt aaatccattc gtgatattcc tttcaccttt
     4801 ttccattttg gcaatcatgc tttgattcaa agacaaatac tcagcaatct tatcttgagt
     4861 aagatttgaa gatattctaa gttcttttat tctttttcca acatcacata catttatcac
     4921 atacatttat catattcata cacctctcat aaaaagaata gcacactctt gtcataattt
     4981 tttaaataaa aaaattatga caaaacaagg aagcaattta ttgatgttgc ttaaaaatct
     5041 aaaattgatg agttcctatc aaaaatcgta tcttcaacct caaaacagta cttaaagcta
     5101 tccgactcgg tttacattgt caaatttaga ttttatttga gcataacttt ctagtttgct
     5161 ttctgatttt tgtttaatat agtagcaaac attgaaggaa atatctccac aaaaaaacgc
     5221 atactattaa gctttttcaa gacctaataa tatgcgctgt tctgatttga aagacattcc
     5281 attattattt tactgtaatc aagccatctg gctctactgt gaattctggc ttgtctgcca
     5341 gtgttccgtc tggtttgagg tagtaccagc ctgttccgtc cgctgactgg ataaaggcat
     5401 ttgataccat ggcgccttct ttagcgtcta agtagtacca agtgtccttg tacttgaccc
     5461 agcctgtctt catggcacct tcttcgttga aatagtacca cttatcagca attttcttcc
     5521 agcctgtagc catttcgcct gagttgtcga accagtacca gttgccgtct gtgtgcttcc
     5581 tccagcggtc tgcaagcata tagcctgaac tgtcaaagta gtaccaagtg ccattgattt
     5641 tctcaaactt gtcttttgga taagagccgt ctgaatgtac gtaccagtag ccagtgtcat
     5701 tcttctgcca gcctgtttca atcgtcaagc cgttctcaat atcatgctta aactgctcac
     5761 ggctaatgcc ccatttagca agatatggat aagggtcaac gtggtctgag tggttgtttg
     5821 gttggttatt cgtgcaatac tcgtgcgttt taattccagc taaactccct gtatcaagcg
     5881 ttttcggcaa acctgcttca tctgctagat tgcgtaagag ttcgatataa aggcggtagt
     5941 ccgtcatgaa ctcttctttg gttgaatggc tttcaatcag ttcaaccgct gcataggtct
     6001 cagcattcca accgccccca acgtcccagg caccattatc aacaggtcct acctgcatga
     6061 tgcaaccgtt cccaacaatg tgcgagaaaa aacctaattc tgggtctttc cgccagtgat
     6121 aatccgcttc attctgtacg gttgaatgcg gattcccagt tgagtgtgcg tgtacttgcc
     6181 tatatggttg cacgccgact tgaggcaaat ctgttcttaa tttactcaca ttaatttcca
     6241 tattctactc cttatcaatt aaaacaactc attttttaca atccagaacc agaaactcct
     6301 ttatttctac cttacaaaga agacaatctt agtcacgatt aggcttgtag atagaacctc
     6361 aaaacgcact attttgacac tgtaaatagg actgacaagg tctgcattct atctacaata
     6421 acaccccaga ctaaaaagct tttcaaaagt atatttttac agtctctata tgtccttttc
     6481 ataaatacta tactttatta tatcatataa aagaagtcaa aagtctgtta aactattttc
     6541 aacaccaaac taaagacgag aacacaagtt ttttcgatgt tcactagagg aaatgaattt
     6601 tattcagtaa atccaactag gattgcactt tggttgccaa aattgccttt ccttctttta
     6661 tcaagggatg acggaatagt gagaagtaca gttgaactgt catggcaacc cagataagag
     6721 gttcacaaag gataacaccc ttatatcctg cccaaggaat aatcaaaacc acaaaaacga
     6781 tttttccgat tagttcaata aagctagaaa ctagaggaag gatcttctgc cccaagccct
     6841 gcaagcaatt gcgataaatc aacaagaggc tcaaaatggg ataaaaggct gaactgattt
     6901 gcagatagag acttccattt tctatcaagt aaccatctgt cgaactagcc aagaaggaaa
     6961 ccaaggctgg actggcaaaa aagaggaaaa tacaaacaaa aactgcccag gatatactta
     7021 aacgactgcc gattcgaaga ccttgaacaa tgcggtcagg tcgcttagct cctagattct
     7081 gagaagcaaa ggtcgtcatt gatgcagaaa tagcggtcat aggaagaagg gcaaaggtca
     7141 taatgcgtcg agctgccgtc tgggcactaa taatcactgc accaaatgta ttaacagaaa
     7201 actgtaaaat cacactgccg atagatacaa ttgaactcat caagcccata gccaaacctt
     7261 gctccaagag atccgcgtac aagcttttgt cccatttgaa atgtttaaac cgtggcaaga
     7321 gttctggcac acttttacga atataataaa agcagagaac cgctgataaa ccttgcgaaa
     7381 taatggtagc aagtcctgcg gattgaactc ccagatgcaa ttgcgtaata aaatagagat
     7441 ccagaaccac attaaccaag gcagagaaaa tcagaaatcc cagggctgct agactgtcac
     7501 caatagaccg caacaagcct gcaaaaagat tataagcaaa gctgacacct acacaggtca
     7561 caatcataga aatatattga taagattgag gaagaatttc tgcaggagta tctaagtatt
     7621 gcaagagagg atacaagcca agaaagccca gcaacataac tagaatgctc aaaagagcac
     7681 ctaaaatcca ggtggctgct actgcttcct tgattttagt gaaattccga gccccataat
     7741 aacgagcaat gacaatcccc atgccattgc caacaccaag tgtaaaacct acaatcaggt
     7801 caaaaatcgc tgtcgtcgct cctactgcag ccaaggattc ttgaccaaga aatcgtccaa
     7861 caatcaagac atcagcagtg ttatagagct gttgaaaaat atttgatagc aagattggaa
     7921 aggtgaagct taagagcgag ggaagaatcg aaccatgtat caggtccact gttcgttttt
     7981 tattcataaa gctattatat cagctttcca gaggagcgac aagaaacagc aaacttatat
     8041 atagcggaat gaaataagga cagtcaaatc gatttttaac aatgttttag aagtagatgt
     8101 gtactattct agtttcgatc tattatagtt ggttgagttt ggaatagtac gctgtagctg
     8161 ctaaaacatt tctagaaatt aatttgactt tcctaataaa gttgttcata tcttatttca
     8221 agctactata tataatctaa aaacttgaat caaaaatcga aaaagtagcg gatttttcca
     8281 ctgctttaac agcttattct tcaatttcga tttcaagttc atcgcctaag tcaagcgtaa
     8341 cttcttcatt cactgcttct tctttctttg gctcatcttt tttgttttca gtatcttgtt
     8401 ctgaaacttc ttctccatca atcaagccaa atttagaacg gacttgctta tcaatttcat
     8461 caaagatttc tgggtgctct gccaagtatt tcttagcatt ctcagaacct tgcccaattt
     8521 tttcatcttt gtaagaatac caagcccctg cttttttgat aatatccaaa tcgcttgcaa
     8581 tcttcaaaag ctcaccagtc ttagaaattc cttctccgta cataatttca actacggctt
     8641 ccttaaacgg tggagctacc ttatttttta caaccttaat cttagtttct ttaccgacat
     8701 tggtttcttt ttggtcacca gttcccttaa tttgtgtatt accacgaaca tccaagcgga
     8761 ctgaagcata gaatttcaaa gcacgtccgc ccggtgttgt ttctggattt ccaaacatca
     8821 ctccaacttt ttcacgcaat tggttgataa aaatggcaat tgttttggtt ttattgatag
     8881 aggcgccaag tttacgcatg gcctggctca tcatacgagc ctgcaaacca acatggctat
     8941 ctccgatatc tccatcaatt tccgcacgag gaacaagggc agcaactgag tcgactacga
     9001 caagatcaac tgcacctgag tcaatcaatt ttcccgcaat ttcaagacct tgctctcctg
     9061 agtctggttg agacaagagc aattcgtcaa tattgacacc aagggccgca gcataagctg
     9121 gatcaagggc atgttccgca tcgataaagg cagcaatccc accttctttt tgcgcttgtg
     9181 caactgcatg aagggcaacc gttgtcttac cagatgactc tgggccatag atttcgatga
     9241 tacgtccctt aggataacca cctgagccaa gggcaatgtc aagagctaaa gaacctgagc
     9301 tcatcacttg caccttttgc tccgcacgtt cacccaaacg catgattgat cctttaccaa
     9361 agtctttctc aatcaattta agagcgtcat tcaaggcctt ttcacgttct gccccaaatt
     9421 tttttgaaat ttcttctaat ttttttggtt ttttcgccat tctattctcc tacattctaa
     9481 tgatcctcag acctatctac tattatatca aaagttagtc acttaataaa gccttgcgaa
     9541 ctaggttaaa ggcatgcata accgcaatgt gacgtacatc tgctcggctt ctgcctccaa
     9601 tattaacctt gataacttca gttccttgat cttgcgccaa gcctatgaag actgtcccaa
     9661 ctgggtgtcc ttctaggcta tctggtcctg ccactccagt caaactaatt ccaaaatcag
     9721 actgggtctt gcttcgtgcc tgctcagcca tcttctgagc tgtaaattca gacaccacac
     9781 catgttcttc caaattcttg gcaggaatat ccaacatcct tgatttttcc tccaagctat
     9841 aggtcacaaa accaccctta aatatacttg aaactccaga aaaattcgcc acggtagctt
     9901 ggaaaagacc tgccgtcaaa ctctctgcag ccgcgatggt tttcccttgc cttttcagtt
     9961 cttctaccac aatgctggct aaactagttt cttccccata accatagcaa aagtctcgta
    10021 aagaaattcc ttcgaaagtc tggcagtcca agatttgatt ttccaagata tccagcgctt
    10081 gattcgcctc ttcttgactg ctagcctttg ttgacagacg tagagtgact tctcctgtct
    10141 tggcataagg ggccaaggta ggatcgatct gattatcaat taaatcagcc aaaatcgtaa
    10201 ccaactggct ctcgccaatc ccaaagaaac gaagaactcg ggaatacagc ttgctccctg
    10261 tcatcaactt gggtagaagt tggtttaaga ccatgggttt caattcactt ggcggacctg
    10321 gaaggacgac ataggtcact ccgtctactt ctaattttcc tccaacagcc agtcctgttt
    10381 cgtttggcag tggaatcgct ccttctacaa tttgagcttg tctttcgtta ttcggtgttc
    10441 gggcatagtc tggtcgcagg gcaaaaaaga tatccaactt ctcctgagcc tgaggatcaa
    10501 agactaatgc tttccctaaa aatttagcta gggtttgttt ggttaggtcg tcctcagttg
    10561 gccccaaacc gcctgtcaaa atcaccagac tgctacgttg actggcaatc tcaagcaaag
    10621 acaagagacg aacttcattg tctcctacag ccgtctgaaa atatacatct accccaatct
    10681 cagctagttt ttccgacaaa aactgggcat tggtgttgac aatctgtcct gtcaaaatct
    10741 ctgttccaac agcaatgatt tctgctttca tgtttcctcc tacctatcta ttcgtatttt
    10801 tttgaaaaaa tcgcaggaat tttcctacga ttgatttttt atttgtatca aaagttaatt
    10861 atcttcatca ccaacaggtg ctctgccaaa taaatcttca aataaaaccg cattggtttc
    10921 aagctgagta acttcttctt gtcccaaaga acgtcggagt atattttgca tttccaacat
    10981 atgtgctctc gaaacaatct ggtaagaaac accttgaagt atctctcctt caccctgcaa
    11041 ctgctgagtt tcaatggttt taaatgaatc tttatagcct agcaagttag ggatactttt
    11101 tgcagacaaa tcaatattgg tctgcatatt gtcactcaaa gcttttaaaa tctcttgata
    11161 atgaccaatg ctatttaaac tgagagcttt ttccatgact ttttgaataa cttcacgttg
    11221 acgtttttga cgaccataat ccccctcagg atcttggtaa cgcattcgtg catagactag
    11281 ggcttcttct cccccaatat gttgctcccc aacaccgata gaaatagtat taaattcttc
    11341 ttggtcactg atagaaattg ggaaacctag gatattattg actgtaatac ctcctactgc
    11401 atccactagt ttttgcaacc ctctcatatt gaccatcaca tagcgatcaa tatggatatt
    11461 catcattttt tgaatggttt ctatagcaag ctctgctcca ccatctgcat atgctgagtt
    11521 cagtttcgct tcatgagcct gaccattccc tgattcaatg cgcgtcagaa tatcccgctc
    11581 taaactcatc attgttgttt ttttcgtttt aggattcact gtcatcaaga tcatgctatc
    11641 acttctaccg acccaagttt cagttcgttc aacatttccg gtgtccactc ccattaacag
    11701 aatggttaga ggttcagtcg cttcaataac cttggtttct tcgccgattt ttttataggt
    11761 tttagctaag gtttctgtcc cttgttgata aatagtataa gcaaaaacac ctactcctac
    11821 tacagttaca gaaagtaaag ctagcaccat tccaataatt tttttaacca tatttctact
    11881 aacctatcag tttacccatc aagtaaacat cgataaattt cccttcttct atatatgccc
    11941 cacgctcttg gctaccttca atgacaaagc catgcttttg ataaagatgg actgctgctt
    12001 gattacgagt ttggacagtc agttggagac gacgcagaat gccacttgct tgtgcccact
    12061 ctatcgcttc ttctagcaac aaacttccca agccattatt ccaatatctt tttccaatca
    12121 caatgaagag atctccaata tgacggactc tcttacgctg atcagctgta atatttacaa
    12181 taccagcaat tttgccattt aagaatgcaa gtaaggttat ctgattgtcc gaactagctt
    12241 gcttgttgag gaatatttcc atctcctcac tagtcaagag aataccatct ccgtctaggc
    12301 tggtaaagtc tgtctccaaa ctcacacgat ttaaaaaggc cactaattca gctgcatctt
    12361 tgggctctgc ttccctaatg agcaattcat actccatatt gaagctcctc taacaatttc
    12421 tcagcacgca aaccctttgc ctgaaaattt aaacggcgtc catctgcttc ttttagaatt
    12481 tccaattcta aataagcatc tggcaaggca tctcctaaga gatttcccca ctcaataaca
    12541 gtcacgccac caccaaagat aaactcatcc aagtcgatag aatcagcatc tccttcaata
    12601 cgataaacat ctaggtgata aagtggaagt cgaccttcat actctctcac gatagtatag
    12661 gtgggacttt taatcatttg agaaatctgt aatccttttg caagtccttt agtaaaggtc
    12721 gttttacctg cacccagttc tccagttaag attaaaacat cattctttgc taatagatgg
    12781 cccaaacgct cccctaaggc ttgcaactct tcttcatttt ttgtgtacat actcttatta
    12841 taccaaaaac ttttcttttg tgtctatttt cctactaaac ttatcatcat aacatccata
    12901 aaaaacaggc tttctctaaa agaaaatgag cgtaacaatg accaatacaa gatctcggaa
    12961 aatatgacca taaaaggaaa cttccttctt aaccgaattt gggacaagat aggctgcaaa
    13021 aaacaagccc agtccaatat aaatcagaag tgagacaatg gtcattggat ttcttaagaa
    13081 aagaagtgtt gctaaaatag tcaccaacac tgtctttttt ctgtctagca tagcaagaaa
    13141 atcgcgcacg tattttttca agggtaaaaa aatcagcaaa tctagcccaa ataggaaaaa
    13201 gaaggatggc aataaaaagt caactaattc ttgctgcagc gtatttttga tgaacaagtt
    13261 atctgacaaa acaagaacag ctcctaacaa attaattaag agtaacatac tgtaaaaaag
    13321 cttcaccgac ttcttactgg ctaggacact atggacttct tgcttacggg tataaagata
    13381 atttactcca gcacagattc ctgaaacgaa aaccatgctt ccgatgaaaa aagctgtact
    13441 ttgtttaaag gacaagatgc attccttcca taggaaacag ctactcaaac tgatttgaat
    13501 taaagctaac aaaaataaga ttctcattga tttcatcttc tctctccctt cctaccaatc
    13561 attatactag gagaaaagag agaactgttt ctaatcttct caaatgtctc tttaagacgc
    13621 taaacaaaca ctagagacta atactcaatg aaaatcaaag atcaaactag gtagctagcc
    13681 acaggttgct caaaacagtg ttttgagatt gcagatagag ctgacgtgat ttgaagagat
    13741 tttcgaagaa tatagatttg aaatcatgaa aatccgtcaa acgggtggtt gttttgtctc
    13801 gcacctcacg gagcgagacg gactcagagt cacataatta taaggctgat agtattaatc
    13861 taactatcag ccttacaggt tatttaacgt ttcagaaaaa ctataatgtc aagattaact
    13921 aaacagtatc tagttccttc aaataatttt ctatcttcat cagcattaaa ggattgttat
    13981 aaatcttgca tagctctctt gcttctatat aataattttt gacttgttct ctgtctagaa
    14041 atttggctcc agcatttcct acaagaataa gtagaggagc caattggtag cttgtctgtc
    14101 tttgtttaca gagttcaatc gtttcaagag cttcttggat ggcttcatta tatttttcct
    14161 ttgatactag gtagtgagcg tagttgtaac gaactctgat gtagccaaat aaaaactctt
    14221 gatggtccaa attttttgtc tgatacaact ctattaaatg agagtagttt gcctcatatt
    14281 cttgttcacg acccactaag gaatagaaat tagatagagt attcaacgcc tttaaataaa
    14341 tcagagtatt tgaagagact tttaataata tattttccaa tgaagaaatt gcctcacact
    14401 tactgtcata ttgatagaag tcaataatag ctttaatcca ttcaaggtaa gttcggtctt
    14461 ctagtgttag aaaagtactt cgttcaatct ctattctata aatatattcc aaatcgtcat
    14521 aatttctatc gtctaatagt cgagcagaca attgcttgaa attagagagg ttagatttaa
    14581 tttcaatttg ttcattaaaa aaataatcta atggtacttc aagtcgttga gagagtttga
    14641 acaaaaggtc tgcggaggga atgaaatgcc ctctctcaat tttactaatc tggctttgtt
    14701 cacaaattcc ttctgcaaga gtttgttggg agagtctcaa ctcttttctt ttcaatctga
    14761 atttctctgc gagtgtattc attaaagata ataaaccttc ctatttgctt aattgcatta
    14821 taaaattttt agttcaaaat agcaagttaa aggaataaat aattctttat ggtaataaac
    14881 taaaaaaagt caaaaattac ataaaaatgc ttgaccgaat aatatatata tattaaaata
    14941 aaaacaagga aaaagaaaag ggtttcacga atgaagaaaa atcgtggaat tcaaaaatta
    15001 gctatattag tattactagg tgtttttatg tttagtaaca caattcctta ccaacagttt
    15061 attcagaaga atagacaatt ggagattcga gtgcaatcac aaaagaagtc caatggtctt
    15121 gatgttggga aggctgattg atttcgtatt actttaaaat tcaactattt agagaaaggt
    15181 attctgatga gagcttgtaa caataccaga tagatataag aatggagatg tttgataaaa
    15241 tgctttcata gctaaccaac tgttgaaaat tatgagttac aaaatctaaa gtatttaaga
    15301 ttggtttata aaattaggtc tctgcctgta gaaaggaacg tgcagttaga aaggaggaaa
    15361 aaataggata ctataattca gactgagatt tctaattaaa atatgtagtt gcatttcgct
    15421 cattttcata atatttcatt cagaggagta ataatatgtt aaaatttaga tttgatgcgg
    15481 gatggtaaag aataattgaa gaagattctt ttatcaatag tttagtaagc ttgttagttt
    15541 atagagggca ggggtatttc tgccctctgt tttagaataa ggaatgcact atgaaaaaat
    15601 tatttatact catatccaac ctgcttgcta gtttgttctt tgtttgggta tttaccattt
    15661 ggactgatac atatgtctcc cattattacc ccaacgttgt tgtacgtgat tcttcccctg
    15721 aaacaacttt tcagcatgtt gctacacgct tggaaaaact agcagaagaa acagatagct
    15781 ttattgcgat tcaacaccaa gatcctaact cagaaggtac tacagttttt tcttatacga
    15841 cctttgggga cggaaagttg cctgatgggc ttcaggaaaa aaatctagaa gatgctcaaa
    15901 gtagtagtgt tgaaacaaac tattttgtat tcgatggaca cctagatatt cacttgctaa
    15961 gggaagagct aagtcaactt ggcttgacta acatgcacct gacaattcca tctaaactat
    16021 ctacgttaat ggctatcttt agtaacggat ttcagttaat cagtctattg attttcatct
    16081 taacctttgt agcactgact ttaattagtc aaattagcca attacggtca tcgggcattc
    16141 gcctcatttc aggagagaaa cggtggtcca ttttccttag accagttggt gaggatttga
    16201 aagcgatagc tgttggtttc agtttagctg gcgtgctcgc cattcttatg caaaaaattc
    16261 tgtcgcttcc aacgcaatcc ctaatgacca taggagcagg cttgctcagt tataatctca
    16321 tcttgttgtc gatctccctg tttttcgctc aactcttcgc agttgggata aaaaagatac
    16381 acctgatgca gattataaaa gggcaagtgc ctgtcagagg aatcattagt ttgattttaa
    16441 tcggtcaact attagcgatt attattgtaa cactgggaat agggagtagc ttaaagtatt
    16501 cccaagcctg gcaacagcat cggattggac aagaggcttg gagtcaggaa aggcaactga
    16561 ttaccctatc aatcagccgt gaaggaacga gtcctggttt tgatgaacaa gctcaaagga
    16621 aactcagaac ttggtatcaa ttgatggatc tggctgtttc agaacaaaag gctttcttat
    16681 ctagacacca gttaattgac cgtactttgc aaaatggcat ggcttcctcc aaaaacttga
    16741 taacctctac agaatggcac gattacaacc cgaatggcaa tgtccttatc gtcacaccgc
    16801 aatacttgga gcgtcaaaac attcctgtag atacaactat tgagcaaaag atgaatcacc
    16861 ttaatatagg ggagtttgtc ttattgctac ctgaacacct ccgttcagag gaagaacatt
    16921 ataaatctgt ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc
    16981 aaatgactgc tacggtaggt tatttggaat caggtcagga tcgatttgtg tataatacga
    17041 cccctatttc ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat
    17101 caactggtcc acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta
    17161 atcaattgtc tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag
    17221 aaatgcaaac aggttaccac aactacatca cattattgga taatatccag agggaacgtt
    17281 gggtaatgct agcaggagct gtgcttggga ttgcaacttc aatcttgttg tttaacacta
    17341 tgaataggct ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc
    17401 tcaggttctt agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg
    17461 gatttgttgc gagtgtattt cttcaggtag agataggagt tgctttctta gtcttgttac
    17521 tctttactgg tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt
    17581 ccatgcttgt tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt
    17641 ggagaacgag agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc
    17701 ttaattggtt caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa
    17761 ccttatgatg ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcaagtgat
    17821 tttttccgtc acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt
    17881 attgaagaaa accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg
    17941 ttgaggcaga agcaggcttt agaacaggtc ggcctggttt atcttgacct agataagcgc
    18001 atctttgagt tatcgggcgg agaatcgcaa cgggttgcct tggcaaaaat tatcttaaag
    18061 aatccaccct ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag
    18121 ttgattatgg agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca
    18181 cataatccgg caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa
    18241 aaatccttgt ttttaattgc acgatgagtt actgaaatat tatcatgaat caagaattgg
    18301 agttaattta gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaagaa
    18361 ataatggaga ggaagttaga atggagagga agttagaatg aaaaaattcg acaattatat
    18421 tattgagaag ccttgcgatt ctaattcaga taaactgcaa aaaatcttaa taattgaaag
    18481 tttggtagat gatattttgc aattttctct cagaatcaat aatagtgtag gagagatttt
    18541 cctcctacaa ccattttaaa agaaaactat ctttattcca tgttattttg aggaagatat
    18601 tgtgaaagtc aaagatgatg ataaagttga gtggaatttg ttagaatttc aaaaatttag
    18661 agcatttttg gcttagtaat ctgtgttgaa ggctcaaaac ctatggtaaa aaagtagctt
    18721 tgaaaacgta ttgcctccaa agatttagtt aaataatgat ttaacacaaa aagaaattat
    18781 tgaagttctg gaaagatgtt gtttcagtat tgagaaaagg tgggaaaaac ttgcgatttt
    18841 cacagagaaa ggaagaaaaa gtatagaaat atagtcaatt gaaacaagaa caggataaaa
    18901 gaaccttttg tgccatattt ttctcctttc gctttacaat tggattgaac acctttattg
    18961 tatcgcgttt ggagtttttt tggtataacc ttcgacgcac acccgcatag cgggtgtttt
    19021 tttgtctcgc acctaacgga gcgagacaaa ctaatagtca cttaatcaaa aaacgcacca
    19081 tatcaaaaac taaaaagttt gatatcatgc gtcatgtctt aaactaattg actatacttt
    19141 ctattcaaat gagcttttaa ccaattgatt gagccaatcc actcttaaaa ccaaagagca
    19201 atttctcgct tagctgactc ttctgaatct gaaccatgta caacattttg gataatctca
    19261 ttttctccag cagcttttgc aaaatcacct cgaatagtgc ctggtaaagc ttcttctgga
    19321 cgagttgcac ccatcatggt ccgccaagtt tcgattactt tgggaccaga aatgacaccc
    19381 acaagaactg gacctgaagt catgaattca cgaatcggtg ggtaaaaact ctgaccaacc
    19441 aagtcctgat agtgctggtc aatcaactct tctgaaacct gtgaacgaaa ctccaatttt
    19501 tcgattgtaa atccacgttg ttcgatgcgc tttaacactt cacccactag tcctcttttt
    19561 acaccatctg gtttgatgat aaagaatgtt tgttccatac tcgtctcctt tgtcagcttc
    19621 tttcttttat tttaccacat ttcgtggaaa aatggagaaa gttttcagaa gagagaatga
    19681 gagaaccctc gggttctctc attctctctt attctactgt ttcttccaca gtttcaacgg
    19741 cagtatccac aactacttct gttgtttctt tatttccttc ttcctctact ggaggattaa
    19801 ggtattcttc ttcgttgaca gcatgtggtt caaggttacg gtaacgggcc ataccagtac
    19861 cagctgggat gatcttaccg atgataacat tttctttaag tccaaggaga tggtctttct
    19921 taccacggat agctgcgtca gtaaggacac gagttgtttc ctggaaggaa gccgctgaca
    19981 agaaactgtt tgtttcaagt gaggctttgg taattcccat aaggactggg cgacctgtcg
    20041 ctggaactcc acctgcgata aggacatctt tgttggcatc tgtaaagtca ttgatatcca
    20101 tgagggtacc catgagaaga tctgtatcac ctggatccat gacacggact ttacggatca
    20161 tttgacgaac cattacctcg atgtgtttgt caccgatttc taccccttgg ctacggtaaa
    20221 ctttttgtac ttcaccgaga aggtacgttt caactgacaa gacatcacga actgcaagga
    20281 gacgttttgg ttggatagaa ccttctgtca gagcagcacc acgcgctact tggcccccaa
    20341 cttcgacacg catacgagct gtaaatggaa cgacatattc accttcgcca gtttcaccct
    20401 taacaaagac tttcttggta cgagttgatg catcttcttc gatagcagta acttgtcctt
    20461 taacctctgt gataaccgct tcccctttag gattgcgggc ttcaaagatt tcttggacac
    20521 gaggaagacc ctgagtgata tcggtatttg aggcaacccc acctgtgtgg aaggtacgca
    20581 ttgtaagctg tgtaccaggt tccccgatag attgggcagc gattgtacca actgcttcac
    20641 caacttcaac cgcatcacca gtcgccaagt tgataccgta acagtgacgg cagacaccgt
    20701 gacgagtgtt acatgtaaat acagaacgga tagtcacttc ttccacacca gcattgacaa
    20761 tttcacgcgc cttgtcttct gtaatcaatt catttggacc aataatcact gcaccagttt
    20821 ctggatgttt aacagttttc ttagtgtaac gaccgttgag acgctcttcg agagactcga
    20881 tcatctcttt tccttctgcg atagaacgga tcaagagacc acggtcagtt ccacagtcgt
    20941 cctcacggat gataacgtct tgggcaacgt cgaccaaacg acgagtcaag taacctgagt
    21001 cggctgtctt aagggccgta tcggtcatac ctttacgagc accgtgagtt gagaagaaca
    21061 tttccaatac cgacaaacct tcgcggaagt ttgaaaggat tggcaattcc atgatacgtc
    21121 cattcggagc agccatcaga ccacgcatac cggcaagctg tgagaagttt gagatgttac
    21181 cacgggctcc agagtccatc atcataacga ttgggttctt aggatcttga ttagcaatca
    21241 agcgtttctc aagtttttca cgggcagcac gccattcagc tgtaacagca ttgtaacgct
    21301 cgtcgtctgt gatcatacca cgacggaatt gtttggtgat ttgttcgaca cgtttgtgtg
    21361 attcttcaat gatttcagcc ttgtcatcaa cgactgggat atcggcaata cccactgtca
    21421 atcctgcaag agttgagtgg tggtaaccga ggttcttcat gcggtcaagt agggcagaag
    21481 tttctgtcgt acggaaacgt ttgaagattt cagcgatgat atttccgagg tttttcttct
    21541 tgaatggagg gttgagctca agattgctga tagcttcctt gatatctcca ccaagtggca
    21601 agaagtattt agctggaaca ccttctgtca agttggcatt gtttggttct tgcaagtatg
    21661 gtagcccctc tggcatgata tcgttgaaga gaattttacc aactgttgta agcaagacct
    21721 tatgtctttg ctcttctgtc caaggcttgt tgaggctgtc tgttgcgata ccaacacgtg
    21781 agtggaggtg aacataacca ttgcggtaag ccataaccgc ttcgtcacgg tctttgaaga
    21841 ccattccttc accttcgcga ccagcttctt ccatggtcaa gtagtagtta cccaaaacca
    21901 tgtcctgaga tggagtaact accggtttcc catctttcgg gttcaagatg tgctcagcag
    21961 ctagcatgag gatacgagct tctgcttgtg cttcttctga aagtggtacg tggatggcca
    22021 tttggtcccc gtcaaagtca gcattgtagg cttcacagac aagtgggtgc aagcgaagag
    22081 ccttaccatc aatcaagact ggctcgaagg cttggatacc caaacggtga agggtcggtg
    22141 cgcggttcaa aagcactggg tgttctttaa tcacttcttc aaggatatcc cagatacgct
    22201 catctccgcg ttccaccaag cgtttagctg ctttgacgtt ttgcacgata tcacgggcaa
    22261 cgatttcacg catgacaaat ggtttaaaga gttcaatcgc catttcacgt ggcacaccac
    22321 attggtacat cttaagagtt ggaccaacgg cgataacgga acgtcctgag aagtcaacac
    22381 gtttaccgag caagttttga cggaagcgtc cttgtttacc tttaagcatg tggctcaatg
    22441 atttcaatgg acggctacct ggtcctgtga ttggacgacc acgacgacca ttgtcaatca
    22501 aagcgtcaac tgcttcttga agcatacgct tctcattttg aacgatgata cctggtgcat
    22561 ttaactcaag caaacgagcc aaacggttgt tacggttgat aacacggcgg taaaggtcat
    22621 tcaagtcaga tgaggcaaaa cggccaccat ccaactgcaa cattggacga agatctggtg
    22681 ggataaccgg aaggatgtta agaatcatcc attcaggttt gtttccagac ttgtaaaagg
    22741 catccaaaac atccaaacga cggatggctt tgacacgttt ttgtccagta gcagttttca
    22801 attcttcttt gagttcagca atttcttttt caagatctac ttgcttcaaa aggtcttgga
    22861 tggcttccgc acccatctta gcaacaaatg aaccataacc atattcacgc aagcgctctc
    22921 ggtattcgcg ctctgtcatg atagacttgt gctcaagtgg tgtatcctta ggatcaatca
    22981 ccacataagc cgcaaagtag ataacttcct cgagggcacg agggctcata tcaagggtca
    23041 agcccatacg gcttggaatc cccttgaagt accagatgtg agatacagga gctttcaatt
    23101 cgatatgtcc catacgctca cgacgaactt tcgtacgcgt tacttcaacc ccacagcggt
    23161 cacaaacaat tcctctgtaa cgaatgcgtt tgtacttacc acaagcacat tcccagtctt
    23221 ttgtaggacc aaagatcact tcatcaaaga gtccttcacg ttctggtttc aaggtacggt
    23281 aattgattgt ttcaggtttt ttgacttctc cataagacca tgaacggact ttacttggag
    23341 aagctagggt gatttgcata cttttaaaac gatttacatc aaccactatt tcttcccttt
    23401 ctattctaag tgaactgctt attcttgttc agcagcttct tctgttgctt ccgcttttgt
    23461 tgctttctca gcttcttcag cttcaaaggc tgctttagcc tcttgggctg ctttttcgcg
    23521 ggctttttca aggtcatcta cgtggatgac atcttcgtcc attccttcat ccaagtcgcg
    23581 aagttccact tcttggtcat cttcgtctag gacacgcatg tcaagaccaa gagattgcaa
    23641 ttctttgaca agaactcgga aggattctgg aacacctggt tttggaattg gtttgccttt
    23701 tgtaatagct tcataggctt tcaaacgtcc gttgatatcg tccgacttgt aagtcaagat
    23761 ttcttgaagg acatttgacg caccgtaggc ttcaagagcc caaacctcca tctcaccgaa
    23821 acgttgtcca ccaaactgag ctttacctcc gagtggttgt tgggtaacag ttgagtaagg
    23881 tccgactgaa cgcgcatgca atttatcgtc aaccatgtgg tggagtttga tcatgtacat
    23941 gactccaaca gaaacacggt tatcaaatgg ttcacctgta cgtccatcgt aaaggattgt
    24001 cttggcatcg ctatccatac ctgcttcttt aacagttgac caaagatctt cagaacttgc
    24061 tccatcaaag actggtgtcg caatgtgaat accaagagta cgagctgcca taccaaggtg
    24121 aagctccata acctgaccga tattcatacg tgatggcacc ccaagtgggt tcaacatgat
    24181 gtcgactgga gttccgtctg gaaggtaagg catgtcttct acaggaacga tacgagagac
    24241 aacccctttg tttccgtgac gtccggccat tttatctccg accttaatct tacgtttttg
    24301 agcgatgtaa acacgaacca acatgttaac acctgattgc aactcatctc catttacacg
    24361 tgtaaagatc ttaacatcac gaacgacacc atcggcaccg tgtggtacac gaagagaagt
    24421 atcacgcact tcacgagact tgtctccaaa gatagcgtgc aagagacgtt cttcagctga
    24481 aagatctttc tcacccttag gtgttacttt acctacaaga atatcacctt ctttaacctc
    24541 agcaccaata cggataatcc ccatttcgtc aaggtctttg agggcatctt caccaacgtt
    24601 tggaatttcg cgagtgattt cttcaggccc aagctttgta tcgcgcgttt ctgattcgta
    24661 ttcttcaagg tgaacagatg tgtagacatc gtccttcacc aagcgttcgc tcatgataac
    24721 ggcatcctcg aagttgtaac cttcccaagt catgtaggca acgattgggt tttgtccaag
    24781 cgccatttct ccattttcca tagaaggtcc gtcagcgatg aaatcgcctt tttcaacgac
    24841 atcaccaact tttacgagag tgcgttggtt gtaagcagta cctgagtttg aacgacggaa
    24901 tttttggatg tggtaaacat ccaatgaacc atcttcacga cgaacttcta ccttgtcagc
    24961 atctgcgtaa gtaactttac catcatactg agcaatcaca gccgcaccag aatcgtgggc
    25021 tgcttggtat tccataccag taccaacgta aggtgcctga ggattaatca atggcacagc
    25081 ctgacgttgc atattggctc ccatgagggc acggttggag tcatcgtttt ccaagaaagg
    25141 aatacatgct gtcgcaacgg caactacctg ttttggtgaa acgtccatgt agtcaacaat
    25201 attagctgga tactcttggt tgaccccttg gtgacgtccc atgacaatct tctcagcaaa
    25261 ggttccatct tcattcagac gagagttagc ctgagctaca gtatattcat cttcttcatc
    25321 agctgtcaac caaacaattt cgttcgtgac aacacctgtt tcacggtcaa ccttacggta
    25381 tggtgtttga acaaaaccat atttgttcaa gtgtccgtaa gatgacaagt tattgatcaa
    25441 accgatgtta ggtccttcag gtgtctcgat tggacacata cgaccatagt gagtgtagtg
    25501 cacgtcacgt acttcatatc cggcacggtc acgagtcaaa ccaccaggtc ctaaggctga
    25561 caaacggcgt ttgtgagaca actcagaaag cgggttgtgt tggtccatga actgtgacaa
    25621 ctgtgatgaa ccaaagaatt ctttaactgc agctgttaca ggacggatat tgataatttg
    25681 ttgtggtgtc aagacttcat tgtcctgaac agacatacgt tcacggacat tacgttccat
    25741 acgagaaagt cccaaacgta cttggttggc aagcaattca ccaaccgcac ggatacgacg
    25801 atttccaagg tggtcgatat catctacacg gccaagtcct tcagccaagt tgaggaagta
    25861 gctcatctca gcaaggatat ctgcaggagt caccgtacga accttgtcat ctgggttagc
    25921 attaccaatg atcgttacga cgcgatctgg atcagttgga gcaataacct tgaatttttg
    25981 aagaacaaca ggctcagtca caacggctgc atcgtttggg atgtagacaa tcttgttcaa
    26041 gtcgccatcc aaatggcttt caatgctttc aatcacgcta cgagtcataa tcgtaccagc
    26101 ttctaccaag atttctccag tttcagggtc taccaatggc tctgcaatgg tttggttgag
    26161 caaacgtgtt ttaacattga gttttttatt gattttgtaa cgaccaactg ctgccaagtc
    26221 atagcgacgt gggtcaaaga agcgagccac aagcaagcta cgtgagcttt cagccgtctt
    26281 aggctcacct ggacgaaggc gttcgtaaat ttctttcaag gcttcgtctg tacgagagtc
    26341 cattggattc ttgtggatat ctttttcaac agtgttgcga accaattcgc tgtcaccaaa
    26401 aatatcaaag atttcatcat cacctgagaa accaagagca cgaaccaagg ttgtaaatgg
    26461 aatcttacga gtacggtcga tacgagtgta ggtgatatct tttgagtcgc tttcaagttc
    26521 caaccaagct ccacggttag ggataacagt tgaaccatag cccaccttac catttttgtc
    26581 tactttgtcg ttaaagtaaa cacctggtga gcggaccaac tgagaaacga taatacgttc
    26641 accaccattg atgatgaaag tacccatttc tgtcatgatt gggaaatcac caaagaaaac
    26701 ttcttgggtc ttgatttcgc ctgtttcttt attgatcaag cggaaggtta caaaaattgg
    26761 tgctgagtag ctagcatcgt gaatacgagc ttcttctagc gtgtattttg gttccttgat
    26821 ttcatatcca acaaattcca actccattgt gtctgtgaag tttgaaattg gcaatacatc
    26881 ttcaaacact tccttaagac cgtggtctag gaaagctttg aatgagtcag tttgaatttc
    26941 aatcaaattt ggtaagtcaa gaacttcttt gattcttgaa aaactacgac gggtacgatg
    27001 tttcccgtat tgaacgtcat gtcctgccaa gatgattctc ctttgtaaat aagttccaag
    27061 ccttgtcaat caggcttttc taatcgtcat atggttgtaa accccttatc accgtgtcct
    27121 cttgacgaat tttcagaatc tttaagcctc tgttacaaat gctcataatc ttgaaaaaaa
    27181 gcacaaaaag agcagctaaa tctgactttt tcagaagatt taactgctgt gagccttgtc
    27241 tggacaatat ttcagacaaa acctacgaca aatgattacc catattatac cctatttagc
    27301 tagatttttc aaggggtttc agtaggtttt tggtaaattt tttcccatag aaaacttggc
    27361 atcacattcg aatcacgcta tggtacaaaa aactgaaaaa actattgact gaaaatcatt
    27421 ttcaaggtat aataataaac gttaaggcgg tatagccaag tggtaaggca cggctctgca
    27481 aaagcttgat cgtcggttca aatccgtcta ccgccttcta taacttgatt tatcaggttt
    27541 caaatgaaca gaaagcccaa tttgaagggc tttttttatt ttccctcgaa taaatacgta
    27601 taactttaaa aacttttgga gcgagtttgt ggcagagttc tttccatggc ataattccct
    27661 tttgaaatca attgaaaaaa gcttcgtcac gcctggtgac aaaagcctct agtttattct
    27721 gtttcttctt ctatttcgac ttctgtgatt tggactttta ccttggtaac acgtccattc
    27781 ttcactttat cattggttaa gataaactgt ttgttttggc tgaccaattc atagctgagt
    27841 ttctcagtcg ttggaatggt tcccactcct gtcaaataat aaccagcgat ggtatcaacg
    27901 tcatcacttt ccagctcaac atcaaagtaa ttattgaaat cattgagggt catagttcct
    27961 tgaacaatgt aggtatcctc gccaatctga tgaacatcta ttgctgcttt gtccgtttca
    28021 tcatcaattt caccaacaat ttcctctaag aggtcttcca aagtcaccaa accagccata
    28081 ccaccgtatt catccagcaa gattgccatt tgtctttggg tatttcgcag ttcttttagc
    28141 aagtcatcca caaaaatagt ttcaggtaca aaaagtggat cttgtaaaat tctcttccaa
    28201 acaatattgt caaaaccgtc cacaaagcct gccttaagga gactcttggt gtgaatgatt
    28261 ccaattacat tgtccttatc cccatcataa actgggatac gagaataatt ttgttttaaa
    28321 atactttgga taatggcttg actatcatcc tgaatatcca ccataaaggc atccgttcga
    28381 ggaaccataa cctctcgtgc catcagttca tcgagcgaaa agacaccttg tagcatctca
    28441 atctcatcag catccaatgt ttcttcacta tttgtcagca tataggcaat ttcatcacgg
    28501 gtcatctttt catccgcatc atcgaatgac ataggagtca aatggctcaa gaaattggtc
    28561 gaagcagcta aaagccaaac aaaaggactg actagttttc cgatcccaat gataatcggc
    28621 gctgtacgaa ttgccaaggc atcctttaga ttaagagcga ttctcttagg atataattcc
    28681 ccaaaaacga tggaaatata ggtcaaaaat gccaaggata gaaaagttgc cacggcttgt
    28741 gctgtttcgc cattcccaag ccaagaggca atcacacgtc ctagagtatc agttaaactc
    28801 gcccctgata agattgtaat cagggtgatt cctacctgaa tggttgataa aaagtggtta
    28861 ggattttcta gtaccttcag caggcggatg tagcgtctgt ctccttcttc cgccttttgt
    28921 tcaactcggg cacgattaag agaaaccatg gccatttctg tggctgagaa aaaggcattt
    28981 aataccgtca agataaacaa taaaacaaat tgtagcaaca aattctgact acttgggtct
    29041 tccatagttc ttctcctaaa atagtatctt atcctttatt atatcacaaa atttaatcca
    29101 atacatatta tttttttctt agcgtaactt ccatctctct cccaaaactg gaagttacgc
    29161 taagacggat ttccactgat ttcacgcgtt tttctcatac taaaagctag gttgtgggaa
    29221 actggaagtt agttttagaa gttactattt tttcttagta cctattctag ccctgcttaa
    29281 actagtaaaa agaactccta catccataag tgacatagga gtttaggctt atcttttact
    29341 gagttacagt tacttctcca gttcggtaat ccagttgaag tcccttttga acattgggaa
    29401 ctagaacatt gaattccaat tctccatccg aagacttggc ttcaatctgt gaagctgagg
    29461 gaactaaatc ctcgtttgaa gcgtagtaaa gggttacacg gtaacggaca cgcttgtttt
    29521 ggtccaaggc tttacgcacc ttgctttcat agtagttttg accagtcgaa tactcggctt
    29581 gtgcctgatt tgcccaggct gtctgaacag caatgttttt aggattgctt gttgaggcat
    29641 caaaaccatc caaaccaccg attaaggcat agcctaacaa atgacctcta tcgactgcat
    29701 gggtataaga gccctttaga ttcttgacct gatgccaacc tggaggagtc caagaagttg
    29761 aaccattccc agtttcttta cgattcttgt actgacgagt ggccttagac aagagggcat
    29821 tagctacggt tggaacagtt tccttgccca ctgtctttgt tttattgtca gcgtagggct
    29881 tacttgaaac cttggcatct agatttgttt tattaccatt gacgataaaa gcacctgagc
    29941 cattccactc cagactcccc tttatttgac tcttgactgc gtctgttaag acactctctg
    30001 ccaatgcttg actaggagct tcagacgctt gttttttctg actaagcttg gttttaggac
    30061 tattagctgt cgactgcatc tgcttgatat aatagctccc tgtagacaaa agcaataaca
    30121 ctagcagtcc gattagtgtc tgtcttgttt ttttgttcat atttctcctt atctttagaa
    30181 aaaggctggt tctccagcct aatttcctgt aaatttatga atcaattcct gccattttgc
    30241 tggagacagg atagcccatg gatcttgacc cttgcccaag attccataac ctaccattaa
    30301 accgattcct agagccagag tacctaaaat cagtactatg atgactaaaa gtaaacgctt
    30361 gactacgtag cttgatttct tattcatctt catcacttgc ctcaatccgc tgaatcttag
    30421 cacctagctg cgccaacttc tcatggaaac cgtagtaacc tctatccaag tgaaccaatt
    30481 taccgaccac agtttctccc tgtgctacca aacctgtcaa aatcaaggct gcactggcac
    30541 gaaggtcagt tgaaagaact tctgctccct gcaaaggctg tccaccaaca atacgagctg
    30601 tatcacggat aatctcagaa tgcaagccca tgcggcgcat ctcttctagg tgttggaaac
    30661 gattttcgaa aactgtctcc accatggttg attcgccttt tgcaactgtc atcaaggctg
    30721 taaattgagc ctgcatatct gttggaaatc ctgggtgggg caaggttttc acatgaacag
    30781 cttttagatt ttctagttga gaacgaacac gaattccttc gtcttcttca attacttcaa
    30841 cacccatttc aagtaacttg gcaatcaagg gacggttgtg ctcccagaca gcgtctcgaa
    30901 tcaagacatc accaccagtc atggcagcag ctaccataaa ggttcctgct tcgatacggt
    30961 cttggactac attgtgagtc gtaccatgaa gtttctcaac accagtaatg gttatagtct
    31021 ctgtaccagc acctttgacc ttggctccca tttcattaag gagaatggct aagtcaacaa
    31081 tctcaggctc acgcgcagca ttctcaatca ctgtcacccc atcagccaga gtcgctgcca
    31141 tcatcaagtt ctgcgttgca ccaacacttg gaaagtccat atagatatga gcgccatgca
    31201 agcgttctgc cttggcttcg atgtaaccag ctgtctgact aatcttaacc cccatagctt
    31261 ccagaccttt caaatgaaga tcaataggac ggctaccaat cgtacaacca cctggcatgg
    31321 ataccttggc atgacccaca cgggcaagga ttggccctaa tacaacgatg gaggcacgca
    31381 tcttgctgac atacttgtaa ggggcttcct cagtgatgtc gccagtagca tccaccttga
    31441 caagatgagc ttcctcatca aagtcaacct tggcattcaa accaccaact acctgattca
    31501 taataaagac atccgacaaa atcggaacat tctgcaagac ggtctttcct tcacttgcta
    31561 gaatagtcgc tgccaacaag ggtaagactg cattttttgc tccctcgatc gtcacgcttc
    31621 ctaccagacg attatcgcca ccttgaacca caattttatc catactcgtt tcctttactc
    31681 ttgatttcat aataatcctc taagcgcttc ttgccacaaa tgatacaggc taataaagaa
    31741 agaactcagg atgtagccta tgacaatgct aaaaaaggct actaataaac gaacttttct
    31801 tgtattatca tctgtcactt ttaaaacctt ctcccatcta acaagatcct ttaaaaggta
    31861 aaaactcaca taaataaaga acatatgact acttagagtg aataataatt gaaccatttg
    31921 actattatac catcttctct gtttgtgcgc tagtttggaa acttcactca aaaattaggg
    31981 attgtttttc aagtaatcaa tcgcatcttg tagggtttta acgggcacga ttttcatatc
    32041 cgtcttgatt gttttagccg cttctagggc tgtttggtag ttgtttttcg cgtccggatg
    32101 cgccttttgt tcttcttcgc taacagggtt atcaggagca aagaaaatag cagcacctgc
    32161 cctagccgaa gctacaacct tcttatcaat acctccaatg tctcccacat taccatcgcg
    32221 gtcaatggta cctgtaccgg caacaatacg accattacga agatctgggt gagctatttg
    32281 agtatagata gctagactaa acatgagacc agcacttgga ccgccaatac cagctgttga
    32341 aaagctaatt gggacattgc tgattacctc tgtacggtca atcaagccga ttccaattcc
    32401 atttttgcca ttttccaagg tgatgatttt tccttctgca gacttggttt gcccatcctc
    32461 ttcataggtg accttgacgg aatcccctaa tttttgagaa ctgacgtaat caatcaagtc
    32521 tttggaacta tcaaaggtct gatcattgac tgctgtgact gtatcagaga tattgagaat
    32581 ccctttaaag gttgaattat ccgtcacatt caaaacataa actccaaagt acttgagttc
    32641 gatatcctta ccagctgttt ttagtccttg atacttggcc atattttgcg atgtttgcat
    32701 gtagaattga ttgattcgca taaattcaac atcggaagaa ccacctgtag tctcctgagc
    32761 actacgaata tctgtaaaag gtgtcaacca agcataaatc atatgagcta aagtggcatg
    32821 ttgaacacca accgtaacga attgataggc accagcttcc ttatcttctg tgtcatttac
    32881 tttaaggact tggcgaatat cttccgaacc acctggaacc tctatataat aaggcaaggg
    32941 cactacaaat gccaagaaag tcacaatcaa ggccgcaatg acgtataagg gccatctaat
    33001 cttttttttc atttcttatt tcctccaaaa tactctcggg aacatagcag gcaatatcct
    33061 gaccaaactt caaaagctct ctaacgcctg atgaactgat atagagatgt tcaggccggc
    33121 tatgtaaata aatagtctct atatcagaag acagctgatg attgtagtaa tcaaaactag
    33181 cttcatattg caaatccgcc gcattcctca acccccgtac taggaaagta gcccccagtc
    33241 tttttgcaac atcgaccacc aatttatcat gagaagccac gacttcaaca ttttccaaat
    33301 gtcccaaagc cttttctagc ccccgtttac gattttcaag agggagaaat ccttgtttgt
    33361 ggggattaaa aaaaataccc acataaagct tatcaaagag tctgctcgcc cgttcaatga
    33421 tatccagatg cccatttgtc atcggatcaa atgagcctgt gaataagcca atcttatctg
    33481 acatagactg tcaccttact aattccataa atcttttcct tccagatacc cagacaggca
    33541 atctcttctg gaagttcaac ggccttatcc gtctcgcaca caaccataac atcttcagaa
    33601 aaaagctctc tctcagccat tttttcaata tctgctacga tttgttcctt ggcataggga
    33661 gggtctaaga aaacgaggtc aaattcccca gatacctgtt ccaatgccct ctctgcatcc
    33721 atcttgagga gttgaaattt tccaacttcc ttggtcatct ggatattttc agccacgatg
    33781 gtctgagcct tacggtctcg ctccaccaaa acagcactgg acatgccacg cgatactgct
    33841 tcgatagata aaccaccact acctgcataa aggtccaaga ctcgtccccc ttcaaagtag
    33901 ggaccaatca tgttaaaaat ggctccccta accttatccg aagtaggtct tgtcgtcttg
    33961 ccttctagtg tcttgagggg acgtccccca tagattcctg atacgatttt catactgttt
    34021 attataccaa attatagaca aaaagagaaa gaaaaccgaa ccttgcggtt cgattctcta
    34081 caaaatattt tcgtaagtat cgcggacttc ttgaggccaa acacttgttt gcacttctcc
    34141 gatgtgtctc ttgcgaagta ggaacatggc catacgagat tgtccaattc ctccaccgat
    34201 tgtcaatggg aatagaccat tcaacaaaga cttgtgccat tccaattcca agcggtcttc
    34261 atcacctgta atttccacct gacgtctaag agtttcttca tctacacgaa ttcccataga
    34321 agacaactca aaggctccac ctaaagactc attccagaca agaatatcac catttagacc
    34381 cttgtagcca ttctcagact cgcttgtcca gtcatcatag tctggtgcac gtccatcgtg
    34441 cggtttacca tctggcaact cgccaccgat accaatcaaa aagacggctc caaattcttt
    34501 acaaatcgca ttttcacgtt ctttcggtgt caagtctggg tagcgttcta ccaattcttc
    34561 tgtatggata aaggtgattt gttttggcaa gatagactcg atgtcatagc gggcttcaac
    34621 agctagctca gtcaggcgaa tagccttata aatcttctca actgtttctt ttagataaac
    34681 gatgttacgc ttaccatttg ggataacctt ctcccagtcc cactggtcaa cataaacaga
    34741 gtgggttgca tccaaggaat cctcatctgg acgaagggct ttcatgtgga caaagagacc
    34801 ctctccttca ccaaagccaa aacgagccaa ggtgtggcgt ttccatttag caagtgagtg
    34861 caccacttca taagtagcat cagggatttg gagaaccttg acagatactg gattttccac
    34921 accagacagg ttgtcctgca ttccgtcacc gaccttactc aagataggac cttgaacttc
    34981 gacaacttct agcttatctt tcaaatactg ggtaaaagtg tttttgacaa aggaaatttc
    35041 ttcttgttga tggataaaac ttttcttcat agacaactcc tcaaaaattt aataaacgta
    35101 ttatacacct atttccaaaa aaagaaaaga gaaaatttaa aaaaatcagt gtcactcaaa
    35161 acactgattt catagacctt ttagtcattg cgaccgaaga tacgtagaat acttaggaaa
    35221 agattgataa aatcaagata gatgctgaga gccattgaca ccacccagcc tgtcgctaca
    35281 cgaccttgtg attgttcata ggcgaggcga atcttttgat tatcccaagc aatcaaccct
    35341 gaaaaaacca aaaccatggc tacgctaatc atataatcaa agaagccact agccaagaaa
    35401 atattgacta ccatagcaat cagtaaacca atgagagctg ccatcattgc tcgaccaatc
    35461 ccactcaagt ctttcttggt aaagatacca actgccgcca tgacaaagaa gagaagggcg
    35521 ctcgatacaa aggcagataa aactgtaccc ggagtataga aagccaccac aaaactaagg
    35581 gtaaagccgt ttaaaacgga gtaaagtaaa aatactggaa gagcagctgg actatttctt
    35641 gaagccatgc tactggcaac gaaaaccaaa gccaactccg aaaaagtagc aatggtcaac
    35701 cagagacgcc cctgcatcaa caagtaaacc aactgagact gaaagaccgt caacataagg
    35761 cctgatacca aagcagatag tccgattccc agaccaacaa aggcataaac cttagcgtaa
    35821 aattgattga gacctgcacg gtcatgtata atagtgtgat tcatcctttt ctccttttct
    35881 gtgaacatct ttccttgatt ataacaaaaa aagttaaaat tagcttaaat ggaagattaa
    35941 aataccggct gcaactgcaa cattcaaact ctcggcctgc cccttcatgc taatatggac
    36001 caactggtct gcactttcag ccataagggg actaattcct tgaccctcat ttcccataac
    36061 tagtacaaaa ttttctatag aaggcagttc tctgtaatca acagaatctt tagatagggt
    36121 tgttgctagc actgggatag ctaccttttt agtttcgtca agaagcgctt gactagtcat
    36181 ccggtaaatg ggcagatgga aatgactacc ttgcatggaa cgtagggttt tgagactgta
    36241 gatgtctgcc gacttatctg aaacaatcac tcctgtaaaa cctgctgcat ccgcagttcg
    36301 aatgatagtt cctacattac caggatcttg cacatcttcc aaaaacaaga acttgccctg
    36361 actcaaatca gcttgtccta cttcttcttt ttgaaccacg gcaacaattc cctgtggagt
    36421 ctgagaatcc gccaaatcta gcaaaatatc ctctgacacc cagacagttt gcgaaaaagt
    36481 agctaactga tctcgataat tttctagggt aaagattttc tcaatcgtca ctccagcttg
    36541 aacagcttct tcaaacaagt gccagccttc aattaaatag gcagacttgc ggtatttttt
    36601 ttggtgtaat ttcttggcat tttttaccac agaattggct tttgaggtta taatagtcat
    36661 agaaatatta taacacaatc aaaggggttt ggtatgcaaa aggttagaat gattgcccaa
    36721 ggtagggtac agggagtcgg ctttcgttgg ggtgtttaca gcttggcact tgaaattggt
    36781 ggcatcacag gtcgagtatg gaataacgac gatggcacag tggaaatctt agcccaagca
    36841 gactcatctg ctatcatggc aaaatttatc caagaaatcc gaaaaggacc gacacctttt
    36901 tcaaaagtaa gctacttaga tgtcaaacta agcaactttc ctccctactc tgattttaaa
    36961 atcgcaaatt aggtctccag aactattgta tattttgtaa aaaaacagta gaatagaaag
    37021 gtataatttt taaagaagga ataaaaacag tgaaatctat taaacgtttt gcactctcag
    37081 ctatgggagt ggctatgttg cttgtcttga ctggctgtgt caatgtcgat aaaaccacag
    37141 gtcagccaac aggatttatt tggaatacga tcggagcgcc tatggctgaa gccatcaagt
    37201 acttcgctac tgataaaggt ctaggctttg gtgtcgctat cattatcgta accattatcg
    37261 tacgcttgat tatcttacca cttggtatct accaatcatg gaaggcaacg cttcactctg
    37321 aaaagatgaa cgccctcaag cacgtccttg agccacacca aacgcgtctc aaagaagcga
    37381 ctactcaaga agaaaaactc gaagcccaac aagctctctt tgctgctcaa aaagagcacg
    37441 gtatcagcat gtttggcggt gtaggatgtt tccctatcct ccttcaaatg cctttcttct
    37501 ctgctatcta ctttgctgcc caacatactg aaggggttgc tcaagcaagc tacctaggca
    37561 ttcctctagg ttctccaagt atgattttgg ttgcctgtgc tggtgtcctt tactatcttc
    37621 aatcgctcct ttcacttcac ggagtagaag atgaaatgca aagagaacaa atcaagaaaa
    37681 tgatttacat gagcccactc atgatcgtcg tcttctccct cttctcacca gctagtgtca
    37741 cactttactg ggttgtcggt ggtttcatga tgattctcca acagtttatc gtcaactata
    37801 tcgttcgtcc aaaacttcgc aaaaaagtcc gtgaagaact agccaagaac ccaccaaaag
    37861 caagtgcttt ctctaaacca agtggacgaa aagacgttac ccctgaacaa ccaactgcta
    37921 tcacaagcaa gaaaaaacac aaaaatcgca acgctggaaa acaacgttcg agataagaag
    37981 aaaaaggctt agctgatttg ctaagtcttt ttgcaaatca aaagcccgat gtttccatca
    38041 ggcttctttt ctatccatgt acacgtttca tataatcttg ataactttcg gtatccatga
    38101 gttttttagc gttcttgacg cgatctgctg ttggtggttt gactccttcg agggaatatg
    38161 gaattccaag ttcacgccac ttgaactcac ccatggtgtg ataaggtaga atttcaaact
    38221 tatcaacatt tttgagggtc ttgacgaact taccaagttc aatcaagtca tcatctctgt
    38281 ctgtcaatcc tggaactagc acgtggcgaa tccagacagg ttttccaata tctgatagat
    38341 actgggcaca agccaagata tttttattag tttggctagt gacaatcttg tgctgttctt
    38401 cgttgatttc cttgatatcc aaaagaacca agtcagtgac agccatgagt ttgtcaaact
    38461 tctcaaggta acgtggttta ttacggaaag gaagagcaca ggtatccaag gtacagtgga
    38521 ttccttgttc cttagccttg gtgaagagag caatcaggaa atcaatctgc aagagagctt
    38581 ctcctccact gactgtaatc ccacccttat ttccccagaa accacggtag cgcaaggcct
    38641 ctgtcaagac atcatctacc gtccgttcac gtgacttatt ggactccata gcccaagtgt
    38701 ctgggttgtg gcaatactgg caacgcatgt gacagccctg caaaaagaca ataaagcgaa
    38761 taccaggccc atctactgac ccaaagcttt ctgtcgaatg caccattcct gtcacttgtc
    38821 cataatcaat tgtttcttca gacatatcgt taccttcctt gaaaacgttt tatagtttta
    38881 ttatatcacg acttgaagga aaaacaagat ttttgcctat tttttagaaa gcaatctgtt
    38941 tttcaatttc attttcaatc tattatcaga ttattcttca aaatcgaagc catataaggt
    39001 tgcaaaatag tcctcagggc gctctgcacg gcggatttga cgggctttac cttcttcggt
    39061 atagaggatt tccgcagaac gtaatttggc attatactgg tagcccattg aaaatccgtg
    39121 ggcacctgta tcatgaatta ccagcaaatc accgatttct gtatgaggca gttcgcgatt
    39181 gactgcaaat ttatcattgt tttcacagag tgaaccaacc acatctaccg cttcagctgg
    39241 tccatctgga tgggtcacgt tgctaatatg atggtaagct ccgtacatag ctggacgcat
    39301 gaggttgact gctgaggcat ccacacctag ataggtacgg taggtttcct tcttatgagt
    39361 cactcttgtg actagagcac cgtgaggtgc cagcataaaa cgacccaatt cggtgaaaat
    39421 cttgacctga ccaagacctg ctgacgtaag aacttcttca tacaccttac gaactccctc
    39481 accaatcaag gcgatatcgt tcggctcctg gtctggacga taattaacac caataccgcc
    39541 agaaagattg ataaagtcta gccaaatgcc caacttttcc ttgatttcaa cagccagttc
    39601 aaagagctga cgagccaact ctggataata gagatgggtc acggtattgg acgctaggaa
    39661 ggagtgaatc ccaaaagtct tggctccttt ttccttcaag atagcaaagg cttcaaagag
    39721 ctggtccttg gtcatgccaa acttagcctc cccaggattg tccataatgt ctgtccccag
    39781 ttcaaaaacg cctccaggat tataacgaca agagatgatt tctggaatac ctgctgctct
    39841 ctccagatgt tcaatatctt caaaggcatc caagttaatg gtcgcaccca attcacgtgc
    39901 ataggcgtat tccttgtctg gcgtgttgtt ggaagagaac ataatctcag aacccagaaa
    39961 gtccagttta tggctcatca aaagctctac ataactagag cagtccacac cacaaccttc
    40021 ttcttggaga attttcaaaa tagctggagt tggagtagcc ttaactgcaa aatattcctt
    40081 aaagcccttg ttccacgaaa aagcttggtt gacggctctt gccttctcac gaatcccctt
    40141 ctcatcatac aagtgaaagg gagtcgggaa ctcggcaaca atcgcttcta actcttctct
    40201 attgataaat ggtgttttca taagcttcct tctattctgt ttgcaaagaa aggctgggac
    40261 aatcgttcca acctttagtt ctatctctta tttatcttcg tcaaagatat tgccaacctc
    40321 aacatcgatg gcttggttct tgacatcaat attagttacc ttcaagagtg acttgtagtc
    40381 aaactgcttt tcacgttctt cttgggcatt gtccgcaaag accgctacac ctgccagttc
    40441 tgagtcgaac tcgcgcaaga gactaatcat accgttgacc gttccgccac ctttcaagaa
    40501 gtcatccaca atcaagacac ggctgcctgc cttaagacta cgttttgaaa ggaacatttt
    40561 ctcgatacgg tcaccacttg aacctgaaac atagttgacg ctaacagttg aaccttcggt
    40621 aattttcagg tcacggcgca caatgacaaa agagacattg aggacattgg caactgcatt
    40681 tgcaagtggc acacccttag ttgctacggt cataaccgcg tcaatttttt ggtccataaa
    40741 gcttttggca ataatacgac caatattttt caagatggct ggtgtgctaa gcaaatcaga
    40801 cagatagata taaccacctg gcaagatacg gtcactttct gacaacttgg tacgcaagtc
    40861 ttcaaccatt tccttagcat cctgactcga aatagacggt gtgaagatga cacctccgcc
    40921 agccccagtc actgtctgga tatgaccgat ttcaatttcc tcaaaggcgc gtttgataat
    40981 gacgatatct tctgagatgg atgatttagc agactcatac ttttcagcaa aagtattgag
    41041 actagttagt ttataaggat tattaatcaa atagttggaa atgacaacca tccgatcact
    41101 tcttcttaat ttcatttcta tttcctattc catttttatt caaaaaatca aaaagcaaac
    41161 tagaaagctg gtcgctggtg gttcaaaaca ctgttttgag attgtcgata gaactgacaa
    41221 accctgtaat atacctgcat atatacatac gacaaggcga tactacccta gtttgaagag
    41281 attttcgaag agtattcatt tttgtctttt acttattata ccatattcac ataaaaaaac
    41341 gaacattctt atcctaaaaa atgctcattt ttcttaaatt atcaatctaa atctggttta
    41401 tagaaggaac gattatccat agcgaagatt ttattggaca tctctccttt atccaccaaa
    41461 gccagagctg ttgacatcat catcatactt gcatccagat tgtcaatcat atggataatc
    41521 tctgcctcca taatacgtgg acggactgga cttccatatt caagcaagcc gtggtgactg
    41581 aggatgacat gacgaagcaa aacgacttct tccttggtat catcgatgcc gagttccata
    41641 actgtcttgg taatttcgct atcaatgaga gcgatatgtc caagaagatt acctcgcact
    41701 gtgtactctg tctggtctgg ccccgtcaac tcgataacct tagctaagtc atgcagcata
    41761 atccccgcat agagtaagct cttattgagc tgaggataaa cttcgctaat agcgtctgcc
    41821 aaacgtacca tggtcgccgt atgataagcc aagcccgttt caaaggcatg gtggttggtc
    41881 ttggcggctg gataggagta gaattcctta tcatacttgg tgtagagatt tcggacaatc
    41941 cgttgccaga caggattttc aattttgaaa atcatttgcg acatgtagtc acgaatttcc
    42001 ttgacatcaa ctggtgactt gaccttgaaa tcagctgggt cattgggttc accagcttga
    42061 ggcaggcgga gagtaatttg attgacttga ggggtattgt tataaacttc tcggcgtcct
    42121 ttcatgtgga caaccttacc tgcggtaaag gcctcaatgt tatgaggttg ggcatcccag
    42181 agcttcccat caatctcgcc actatcatct tggaaggtaa aggctaggta gtttttccca
    42241 gctcgagttt gcctcaggtc agctgatttg attaggtaaa agccttcaaa taactcatct
    42301 tttttcatgt gactaatctt catattcttc ctcattttct tgaaaataga gtagatcaag
    42361 cgcaggctca ccttctgaca actcaatgtg acggagcgtc cgctcgatag ctatggtacg
    42421 acggtttaat aattcatcaa tattgccaga ggcatgttgg agatgttttt gtgccttgac
    42481 cagaatacca ccaaacttgc caaactcggt cttgacactg gcaagagtct tgctgatatg
    42541 gtcggcactc ttttggatat taagggtctt gaaaccaact gatagggagt taagaagggc
    42601 tgatagggta cttggtcctg caacaataat ctgttcttcc cgtctcaaat catcaaagaa
    42661 gaccggattg cgcacaatct ctgaataaag accttctgtc ggaacaaaca agattccaaa
    42721 gttggttgtc cgaggcggtg ctatgtactt gctcttgata tccttggcaa agcgcttgac
    42781 gcttgctagg agtgacttac gacagcgttc aatctcatcc ttgtcacctg tctcataggc
    42841 ttcttccaag cggtaataat ctgccagtgg aaacttagag tcaattggaa gatagacgta
    42901 ttcttggtcg ccttgtccgg gtaacttgat ggcatactcc actcgttcac tagagttttc
    42961 aaccgttgcg tattctcgtt cgtactgggc aggtgtcatg atgtcttcaa taatttgccc
    43021 cagttgcaat tctcccagaa tccctcgcgt cttggttcca gagagaacct tgttaagagc
    43081 tccgacatca cgggcaactg tctgcatttc tccaagacca cgattgacag actccagttg
    43141 tttagaaact gtctcaaagg aagcctgtaa gcgtgtctgc aaggtctttt ctagtttttc
    43201 ctcgaccgtc tggcgcattt gttccaaacg ttgctcattt gattcctgca aggcttggag
    43261 acgttggtct gtcttatctc tagtttggag gagattatct gtcatttctt gacggacttg
    43321 ggtcaggcct tggtgcaatt caatccgcac ttcttgcaaa cggtcgctga cagccacttc
    43381 caaatctttt tggtctaact ggctggcttg tctggcttgg tcaaagcggt aatccaactg
    43441 gtctgacaaa tgatctgcct gatcctccaa gctcttactt aagtgtttct cctgcctatc
    43501 ctgcctttgc caaatcagaa agagaccagc taggttggca attaataata gaataagtaa
    43561 actttccatc ctacctccta tccttgctat gcagtacgac cacataacca tctgggcaag
    43621 ttaccgacac ttccctatct atatattcgt tagaagcgta cactttttta aagaaaaaat
    43681 tttcctctgt caactcatac ttggctccaa gaatagtcag ctggctatcc cgaactggca
    43741 taaaggctag atagtcgtag tctgaacgag gttctagctg actggtccct tctggacaat
    43801 aagtaatcaa gttttgccca tcctcaattt ctatttgatg catatagggt gccaacttag
    43861 gattgctagg cagaaagaca ttggccaaca tatggtcaat acggccaccc aaggcaccga
    43921 aaatagtaac ctgagcctga ggatttttct caaagatggt taagagagcc aattccaaat
    43981 cggtatcatc tttttctggt cgggcttgga caaaatactg ggcacgtttt tgaatcacct
    44041 gtcgctcttc ttccgtcaca gaatcaaaat ctccgactgc tagagcaaga ggtaagtctt
    44101 cttccaagac ccagagcgag cctcgatcca ccccaacaaa agcatcaaaa tctgtccgat
    44161 aatgaccgcg gtttccgcct gcaaaaactg caaccctagt ccagttgttt tctgagagtt
    44221 tgtactcgct cattgacttc tcccttaaag acataggaac ctgctacaaa aacagtcgca
    44281 ccagcttctt tggcttgagc aatagtttgg tcatcaatcc caccatccac ttcgatttca
    44341 aagttcaaac ctttttcctc acgaagagca accaactcac gaaccttatc catggtttct
    44401 ggcagaaagg cttgcccacc aaaacctgga ttaaccgtca tgactaaaac ttggtcaact
    44461 agatgaagga cgtgcttgat ggcttcaact ggtgtgccag gattgataac gactgaaggc
    44521 ttaactccga gtgaacgaat tttttggagg gcgccatgaa tatgaggtgt tgcttctaca
    44581 tggatactga tgatgtctgc acctgcacgc gcaaaatctt ccagatgatg ctcagggttt
    44641 gacaccatca agtggcaatc gaaaaccatc ttactatgag gacgaaggct ctcgaccaca
    44701 cctgcaccaa aactgatttg cggtacaaaa tgactgtcca tgatatcgat atgggcatat
    44761 tctgccccag ttgcttctag acgtttgatt tcacgttcaa agttggcata atctgctgcc
    44821 agaattgacg gagcaatctt gtattgagac ataggtttct ccttattttg gaattttttt
    44881 gctgactttt ttataggttt ctctacgatt ttcaatttca ctaaggaatt gcaggtaatt
    44941 gtcaaaacgg aaggttgcaa taacaccctc ttcaacagct ggtttgacgg cacaagacgg
    45001 ctcatgggta tgggtacaag tacggaactt acaatctcgg ctaacagtag caatctctgg
    45061 gaaagcctga ttgaggtctt cagcccttga tacttcatag tccaaggatg aaaatcctgg
    45121 tgtatctgcg attttacccc cgttgagatt gtaaaaacta acagctcgag tggtatggcg
    45181 accgcgacct agactgtctg aaatttctcc cgtttcaaga ttgaggtctg gtgcgatttt
    45241 attgagaaga gttgacttcc caacacctgt ctgccccata aagaccgtaa ccttgcctgt
    45301 taacaaagac aggagttcct ctttactggt cacaaagtca tagccgatgt caccataggt
    45361 ctgctggtaa aaatccagtt ctcccctatc ttccaacaaa tccattttgg aaatatagac
    45421 aatgggatgg atgcccttgt gctccaaaag aaccaagaaa cgatccagca aattgctgtt
    45481 aaaatcaggt tccttgacgg acatgattac tacagcttgg tcgatattga caataggcgg
    45541 acggaccaga ctgtttttcc gttcgtgaat cttgagaata tagccttctg aattttcctc
    45601 ggcagagaaa tctacccagt ccccaacgta aggggtatgc ccttttttac ggaaattccc
    45661 acgcgcgcgt gtttgataga cttggccatc actctctaca tagtagaacc cagccaaggc
    45721 tttaatgatt tgtccctgca tcttagagtc cttgcccttt aagtgcgtct gctagaccgg
    45781 caaattctgc caagctgaga gcttccccac gcacacttgg cgacaagtct gcctggtcca
    45841 aagccttggt cagcttgtcc ttgacttcct cagtcttgcc aaagtaacct gtcaagttat
    45901 tccacaaggt cttacggcga tgggtaaaac tagccttgga aaccttaaag aagaagttct
    45961 catcttccac tgctacagct ggctctggac ggcgcaccat tttcaagatg gctgaatcca
    46021 catttggcgc tggcacaaag accgtacgag gcacgataaa ggcaaccttg gctgtcatgt
    46081 aatactgcac ggcgatagac aagctaccgt aagccttggt gttaggctgg gctgaaatgc
    46141 ggtccgctac ttctttctgc atcatgacca caaactcact aaaaggaatg ccactctcaa
    46201 tcaagtgcat gagaataggc gtcgtgatgt agtaaggcaa attagccact accttgattg
    46261 gcaggttagg atttttaaaa ttttggatat gttgcgccaa atcaaccttg agaatatctt
    46321 cgttaactac agtcacatta tcaaaatcac gcagggtatc tgccaaaatt ggcaccaaac
    46381 ggtggtcaat ctcaaaagcc atgacttggg ctgcacgctc agccaaaaat tctgtcaagg
    46441 caccaatacc tggcccgatt tcgatgacat tgacctgatc atcaatttca gccgtatcca
    46501 caattttttg aaggatattg gtatccgtca agaaattttg cccaaaggac tttttaaagg
    46561 taaaaccgtg acgctccaac actgccttgg tcacgctata atctgcaatt ctcatctatt
    46621 ctcttttcta ttatctgtta ctactattgt aacacattcc ccttgtattt tgggaatgaa
    46681 aatcatacca aaaaactatc tttcctcatg aagaaagata gtcttggaaa tctttaaaag
    46741 tggccttaac agttctctcg aaacatgcgt tcaaaaaaat cagcttcttg ataacttcga
    46801 ttttcgggta gggtcaaatc cacatcctcc tccacatcat ccactgtcag ttcaaccgtc
    46861 tgaggtaaat ttttcaaacg ataatgcttt tgcctttgcc cttcaatact agcaaaatca
    46921 agcaaatagc catgcactcg gatttgaacc aaaaggagat gattggtcat attttccatg
    46981 atgaggatat cttctaaacc acctctggca ctaggataat cagataccct gcgaatacgg
    47041 attttttcac acatgagcaa gtttcctcat atcaatgatt tcatcagcct tattccagac
    47101 taggggatta tgggtcgcaa tgatgataat tcgattttca tctttcaaat ccaccaagag
    47161 attcataacc tcctctgaat tttcaggatc aagagctgct gttggttcat ctgccaaaat
    47221 caagggtgga tttttcaaaa tagtttttgc aagggcaact cgttgggcct ctcccccaga
    47281 taaagtataa attttttgtt ctaaatccaa ataccctaga ttaacttttt ctaaagcccc
    47341 cacttgcctt tccaaacgtt ctacttttga gattttctga ccaacaaaac ccaaatccaa
    47401 attttctttg attgattggt tttctaagag gccaaaattt tgaaagagat agcccatctg
    47461 atctcgaaaa tactcacgag tgggaatggt ttttaaatct ttcccctgat agagaaccct
    47521 tcctccatct atcttttcta gctttcccaa gatattcagc agcgtcgtct ttccgcttcc
    47581 actctttccg attaaggcat aaaccttgcc cttctccagc ttgagattca aaccagagaa
    47641 aatcgcgcgg tcattaaatt tcttttccaa tccttgaata tcaatcatct tattttcctt
    47701 tcaatactgc catgctacca gcttcttctt ttttgtcctg ccttactaaa atcaagaggg
    47761 cgttaagcgt aaacaaagct acaactagag cgctaatcaa accatctctt gtcaaaatac
    47821 tagataggac taggccaagc aagagaactc ctccttgcgc cagtaaatac ttgccatgaa
    47881 gctcatagat tgtcatacca gcaagacgtt taatcataag atcccgtctg aactgctcaa
    47941 aatagagaag attcatggaa tcaaataaca agatagccgt agacagggtc aaaatcgtcc
    48001 caataaggag agagtaaaac tccacccgtt tgtcatttag taccttggca aaaaatagct
    48061 ggctttttac caagtaagaa accttgtgat acagaccttg ctcttttaga gctgtgatgc
    48121 tgtctccata tcgatctagt ttcaaggcac tattagccgt agttccccac aacatccctg
    48181 caacaggtct tgttccagtc gcttgcggcg ttagaactac aataatcgga tcttttaggt
    48241 actggcgtgt tgtcttgtac ccatcattat agaggaaacg ttcctgtcct atttctgtaa
    48301 aagctagcct tacctgtggg aggtagtgtt tctgactcgt cacttctaca ctttcagatg
    48361 aaaagttttg caggtaatct gtaaacaatc cttggtagta gatagactgc tctcgcaagc
    48421 tctcaggcaa gatcagccca aactctccct cagacaagtt ttgcatcttg tccataaact
    48481 ctgaagaaac ggtaatcttt tcttctatca gatagcgcgg tgagacatag ataacattcc
    48541 ctgacggtgt gtagtcactg agacgattgc catctaggtc cacttctgct ccatctgaga
    48601 aattgtcaac attgctcata atataaaaag agtctgtatt ggctaaccgt tcttcagtaa
    48661 atgtctgcca ctcacgatta tccttacgcg ttccttcttc atcggcaaat gcactagacc
    48721 aaccaaagga tagacggtaa cggtctgagg actggctcca tttattgcta gctctctcca
    48781 tttcctgcat ttcacggtag tggggtagga gagctgtcgc actcgatccg accaccaata
    48841 cagctaagag ttgccccacc atcatcaatg tcatcatacg tttgagaggg agtttccctt
    48901 tcaatagatc caccagacta ttttcctgta aaccaagtag atagacgaca cttagtaagg
    48961 tagaaatccc tgccaaggtc aatccataaa gtagaagagc aatgatgacc agttgcaccg
    49021 ttgccataaa caaggcacct tgataccaga gaatccccaa tcccaaaaga ctggatacca
    49081 gcactgagca gataagctgt ctcacatctt ctaacactgg tctgagagca actccaaaca
    49141 agctctcacc agctattaag cgaatccctg cctgacgaag ggatttgatc cgataaatca
    49201 gggtcagact cataaaggtc agcagaaaaa tagctaaact cagtagcacc ataggggtgg
    49261 ccgtcaatag taagactatc gaaaatggat cttctccatt cgaaacaaag ccttgataac
    49321 caagctcttt caaggtggtc tgtaagctca ctccatccaa acttcctgat acaatcaagt
    49381 aagaccctaa taaatcactc gtttctgcac tctccttgga ggaaatggtc agaccttctg
    49441 gaagctttcc ctcaccataa gtggcatagg taaagcgtgt ttctccagct tcatttggct
    49501 caacgattcg acgagcaatg agactattgt gctctaccgc caagtgactc aaagcctctg
    49561 tcacctcttc gcgactagct actggttgat ttatcatcat tcctgtcaca tcaacagaag
    49621 gatagttacc aaataaaatc tcttctcgat ggtcaacgga agttatcacc atataaagcg
    49681 atactaatac cattgaaatc aaaataaata aacgtttcat catttcctcc aaaaagttac
    49741 ttggtcactt agtgagaagg tgaccaagta aagctaaaca atagggctat accattctaa
    49801 acattatagt agaatgcaac tttctctccc caagaagtac gaatccatgc acgagaagtc
    49861 cctccagcac cagcatatcc cttgtcagat gaaccagttt ttctgctaac aactgttgaa
    49921 ctatggtatt ttgaagggtg atagtagttt gagtaagcac caccgttacc tgaaccataa
    49981 gtccaaacac caccttctgg gtattgaacg gctgcagagg caactgctac acttccacct
    50041 aatgttgctg caatgctcgc tacaagagct aattttttaa ctgtttgttt catattgata
    50101 ctcctctatt tttgattaca agattgttga gttacttgct cctctttcca ttatcaacgt
    50161 tgatactatt atattaccaa atgatattct aaaattattt gtttttctct aaattgtcaa
    50221 aatagcaccc tgaaatgaac ttttatagtc attatactcc atctttatca cactatcaag
    50281 gacatcaatg tccttaaaat ctaactattt catattattt ttagcttata tctaaaaaaa
    50341 cagttctaaa gacgaacttt agaactgttt tgatacgagg acaaggacat ttttgtcact
    50401 gattcactat gtttataaat atttttttaa atcttcttgc cattgttttt ctaacatagt
    50461 aactaaataa gaattttcta ttatatcagc aaatagcttc gatttctgat agaactgttc
    50521 ggcagaaaca taatcattgt ttattattag agataatttc cactccaaca ttaatataat
    50581 tggtttcttc tggaaatctt gtgttttttc cattatatga ttaaggctaa agatataagc
    50641 tcctagtgaa tcataagacg acaaattatc caaacaggct agacccgaaa acaaagtgtt
    50701 acttaaagca aacagatatt ctggtgcaat taattctaca tgatttgtca aacgaactaa
    50761 aaacgaatga attctgttaa tttcttcaat ctgattttga ccttgtttta tctttgtaag
    50821 cactatagca aaatataagt tgataacttc taattcattt atcaaaaaca attccttatc
    50881 ataaattgtt ttaaaataat cgtccagtac ggtttccccg tattctggtt gtccgcttcg
    50941 aataacatct atcgttgctc tagtagcttc aaaccatact ttctcctcat taggaagttg
    51001 ctcataaaat aaatcctcta tctcttccaa acacgcttct ttcttatcat actcctcttc
    51061 tttatcatag attggttcac gtaaaatctg gtatttcaat tctaaataag cagaaggaag
    51121 agctgaaaaa tccggcataa ggctataaac tggaactcct agccggcgag caatatagtc
    51181 taacttgggc aaactcggtt gcgaagctcc actttcaatt cgcgacagtt gacgaactgt
    51241 cagttcttgc tcatctccac aaaaatctgg acgactaatt cctttttcta tccgaatctc
    51301 tttaattttt tgaccaatta acatcagacg cctcctcttc tacctgcttc gaaaaatact
    51361 gtgaaactta aaaaattgta acggctacat ctttcctact cataagattt catagcttct
    51421 tccacttctg ccaaagtaac cccaaacaac tctaggcgtt tgaggagttg cttgccgtta
    51481 gaatagccga ttcggagagt ctctccgaga tattctctac gcttacggct gtctgcccct
    51541 gctagaaaac caaggcgaat caaatcgcta cgactaatgt caaactgact ctcatgttca
    51601 aattgttctg tcacctgagc tagagccgtt ttcaggtctt catagctggc atgctcaatt
    51661 cccagagaac gccccttggt cttggacttg ggaacagctt catctcgctt gagaaaggca
    51721 tgctgaactg ttggaatggc cgtcatgatc atgcgccgaa tccgctcccc attaaaatct
    51781 gggtctgtaa agacaatgac tccatgacgt tggtgtagac gctgaattcg ctctatatcc
    51841 tgatcattga tggcagaacc tcgtgtctca taggtctcca catcgaaata acgcttgaga
    51901 ttgaccgtat catcgcgacc ttcaaccacg ataacttgag aaattttctc tttcattact
    51961 tgctgtccaa tccaaaaatt cgttctgcat ttgcagtcgt tgctaccgcc agctcttctg
    52021 tcgtcatacc acgcaagtca gcgataaagt cgaccacata gcgagtatag gctgttttat
    52081 tttcacgacc acgcttgggt acaggtgcta agtaaggcgc atctgtttcc accaacatct
    52141 tgtccaaagg taactcttta gctgcttctt ggaggtcagt tgccttcttg aaagtcacca
    52201 ctcctgagaa ggaaatggtc ataccaagat ccacaaactt ctctgcccac tcaagcgtcc
    52261 ctgaaaatga atgcatgata ccaccacgag gaccaacgcc ctcactcttg ataatctcat
    52321 aggtatcttc cagcgcatca cgggtatgga caacaaaagg caaatccaag tccttagata
    52381 gctgaatctg acggcgaaaa acctgctcct gcacctcttt aggcgctgtc atccaatggt
    52441 agtctaagcc aatttcacct aaagccacaa ccttgggatg ttttaactta tccaacaagt
    52501 aagcctcaac ttcctctgta taagtaccag cttctgtagg atgccaacca atagtcgcat
    52561 agagctgctc atactcatct accaactcca aggcacgctc aatcgtcggt ttatcaaaac
    52621 caacaatatt catctgtgtc acacccatct catcagccaa ggcaatttct tctgcctcac
    52681 gacctgcaaa ttcttctaca ttcaagtgtg tatgtgtatc aaaaatcatc tcttctaacc
    52741 tcgttttcta tcttctatta taccaaaaaa gaggaggggc acctaatttt tcggtttccc
    52801 ctcctctctt caatagagag ctattctgct atcttttcta tccgatattg cccatctcct
    52861 attccacagt tagagacaga agagattggc tacattgata gaatattcct attcagctaa
    52921 agtatgtgat tatcttgggt tctcactttt ctcacccatc actaggattg atgacttctc
    52981 aaaatccccc ttataatcta actttaagta gagaatagaa ttttctagct gttctatttt
    53041 ctacgtctag tctttcttcc tagcaaagca aaaccacttg ttgccaccac tagaccaaga
    53101 ctagcaagtg ctgcacttga tgctgaacct gtgttaggta attgtttgcc agcaggtgac
    53161 tggtaggtaa catcttgcat tgttcctgca gttgcttgcg gtgcacttgt tgtcttagga
    53221 gccactggtc tcttaggaac tgtaaccatg gcaatggctt gcactccaat cgtttctgct
    53281 tccttcacat cttccatagt tttcgcattt tcaatctctt tgagagctgc ttgtttttct
    53341 gcttccactc ttgctaaaag ttctgctttt tctttatcag aaagcggtgc gcctttgatt
    53401 tcatcctgct tgtccttggc tgctttttca atggcatttt tagctgattc tttttcagta
    53461 gctaattgct tagaagcttg tgattgagct gagttttgtg aacctgtgtg agctgatcca
    53521 cccgcctggc taccaccgcc tgcattcgca ttagcatctg agtttccagt attgcctccc
    53581 ttatctccac ctggtgtagt ttctggctta tactctggtg tagcttcttg agttacagat
    53641 tcacctttcg cagattgttg aactgtatct tttcctagaa tcgtcactac tgaaccatct
    53701 gggaatgtga ttgttgctgt gccatctcca gctacttcaa ctttatagta aaataaaata
    53761 ataacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaaacaga
    53821 ggtgtactat tctagtttca atctactata acatcgcttc cgaatccatt tgcatctact
    53881 gaactttttt acaaaaataa aaatcagtag actcgagttc ctactgattt ttgatagaat
    53941 tcgtttggat tatgcagcca aaactttgcg tcctttacga cgacgagctg ccaatacgcg
    54001 acgaccattt ttagttgaca tacggttacg gaatccgtgt ttgcgcgcac gacgaagttt
    54061 acttggttga taagtacgtt tcacgatgaa tacctcctca tagattttgt attcgtttag
    54121 ccggctataa agtgatcagt tactaaacat actttactat tctatctgat tctttcactt
    54181 ttgtcaatag ctctaggcaa atgtttccag ctttttcgaa tgcaagccct atctacgata
    54241 ctgtttcaag aaacgagtca tcttttcttg gatttgggct aactcatcaa cacgaggaag
    54301 gtaaacgata cggaagtggt ctggttcctg ccagttaaag cctcgaccat gaaccaagag
    54361 aaccttttcc tgcttcaaga aatcaaggac aaactgctca tcatcatcga tacggtacat
    54421 attgcggtcg atttttggga agatatagag ccccgccttg ggtttaacgg cagacaaacc
    54481 tggaatatct tgaatggcat tatagatgaa atttctttgc tcgtagattc gtccaccagg
    54541 aagaagcaat tcatcgactg attggtgacc ccccaaggaa gtttgtacga cttgttgggc
    54601 caaaacgtta gagcaaaggc gcatattgga cagcatattg agcccttcga tatagccctt
    54661 aacatgagtc ttagggccag acaagaccat ccaacccaca cggaaacctg ctatgcggtg
    54721 ggattttgac agaccattca tgctgacaca gaagacatct ggtgccaagc tcgccacagg
    54781 cgtatgcaca tgtccgtcca ttaccatgcg gtcatagatt tcatccgcaa agataatcaa
    54841 atcgttttga cgggcaatct caataatctc cagcaagagt tccttaggat aaagggctcc
    54901 agttgggtta tttggattga taaggacaat tgccttggta ttggaagtga tttttgactt
    54961 gatatcgtca atatctgggt accattccac agcttcatca cagatatagt gaacggcatt
    55021 tcctccagct aggctgacag ccgccgtcca tagaggatag tctggcattg gcactaagac
    55081 ctcatcccca ttgtccaaaa gcccctgcat ggacatgaca atcaactcac tgacaccatt
    55141 tccaaggtaa atatcatcaa tatctacgtt tgggaatttc ttcagctggc aatactgcat
    55201 gatggcctta cgggctgaga aaatcccttt ggagtcagaa tagccttcac tatcacgtgc
    55261 attcataatc aagtcatgaa tgacctcgtc tggcgctgta aagccaaatt ctgctggatt
    55321 tcctgtattc agacgtaaaa tcttttctcc attcgctcgc atccgcatgg cttcttccaa
    55381 tacaggacca cggatatcat aagcaacatg ctctaactta ctagacttgt tatattcttt
    55441 catcttgttt cctcaattca tcaggatagt aacattatac cactagaaag agaatgcgac
    55501 aagtttactg aaatgtgtta ctagtttcgt atagaaaaaa aaccttgcta aaggcgaggt
    55561 taagaacgta gagatagcag gtattttgca aagacacatc acataccaaa agataaacct
    55621 agaaaaagca agcctagaaa ggagtcataa agaagattaa acatgaggaa aagcccccat
    55681 atcatcaagt aatcccaacg cccacttcgt tttgcaacta gaaataagat tagatggtag
    55741 attagatggt agagtacatg aaagactaaa aatccaataa aacctggata aagaagcggt
    55801 accagactct ctaattgcca gatcatgatc acttctacca agactgaaac taggataatt
    55861 ccataataca ggaaatttga tttttgaaaa gagtttttca tcattctccc ttccttcact
    55921 tctttcctgc tatcctttct tttattttat catatattta tatagatttt aagccctaat
    55981 attcttttga aaaatacttt ttttgcttta cttctatagt gaaaaggttt acaattatag
    56041 taagaaaatt tcaggaggtt tcattatggc acaacgttac caaaatatca tggtcgcaat
    56101 cgatggttct aaggaagcgg acttggcttt tgtcaaggga gttcattctg ctctacgaaa
    56161 cgacgctaaa ctcaccatcg cacatgttat tgacacacgc gctctccaaa gcgtatccac
    56221 ctttgatgct gaagtttacg aagaactcca agtcgacgct gaaagtctga tgaaagagta
    56281 cgaaaaacgt gctaaagatg ctggggtagc agatgttcat atcgtcattg aaatgggaaa
    56341 tccaaagacc ctgctagcac gtactattcc agatgccgag gaagtggacc tcatcctcgt
    56401 tggcgcaact ggtctcaacg cctttgaacg cctcttggtc ggctcttcat ctgaatacat
    56461 actccgccat gctaaggtcg atttactggt tgtgagagaa caagaaaaaa ccttataatc
    56521 acaaagaaaa ggagccccta gctccttttt gtttacgatt tatttctctc tttatggcgt
    56581 tcgtaagcct tgagctggcg ctgcagttcc tttttaatag caggttctgg agcatatttt
    56641 tcttcccaat tatctggttt taagatttta tgggtcactg gatcaaaatg agccttgcca
    56701 tctggaaaaa ttttccccat attggcctga tggacaatat caaaaatacg ttctgggtcc
    56761 acccccatca agacaaaact gccgtaggtg aagtaaagcg tgtcaatcaa ggcatccact
    56821 tgccctatca aatcttgctg agcaggtgtc ttcttggcta ctttatctgc tgccttatca
    56881 agggcctgat gaagttgcga tacagcttga ccaaaatctt cttcagaagg actggctgct
    56941 cgaacaaact ccaccaattc ttctatttta aaaccagccc tatgggttgc accctctaaa
    57001 tcccaagctc gaggttcttc ttgggttcgt tcatccatca tgtggtggaa ggtcttgacc
    57061 ttattgaaat gatagtcacg gctgacaaag actttttctg aagacaaaac accaaaatgt
    57121 tccaaggcct tgtggattcc atcttgttga ttgctagtgg taatgtgctt ggcagcttcc
    57181 ttgacactgc tactaccatt tcccatagcg accgacatac cgacgccagc cagcatttca
    57241 aggtcattat ctgagtcacc aaaagccatg acttggttga ggtcaaagcc atattctttc
    57301 ccaactcggc gaatgccttc taatttagaa tttccctgat tgatgacatc cgatgcaaaa
    57361 ggattgctac gtgtcaattt caagtcttca aaatcagctg ccgccttctc agattcttct
    57421 ggtgtcatca acatcaaaac ttggtagata ggctgattca tcaggtgaag caggtcctct
    57481 tccttttggg gaacaacctt gctgaccatg cgattaaaag actgactcac cgtccgagtt
    57541 aaaacagagg gaacgaagcg actaattcgt tgggaaaaag aacccagacc aaaggacatg
    57601 attttagaac ccaacatggc atccttggtc cctagagcaa tctccgtgcc ctctttttta
    57661 gcatagctaa ttagatggcg caaatgtaac ttggaaatag ggctcgtgaa caagactctg
    57721 tctttactaa agatatactg gccattatag gttaccgcaa aatccagatc caaatcgtcc
    57781 atcaattcct taacaaaaaa aggtcctcgc cctgtcgcta cgccaactag taccccttgt
    57841 tctttgacaa tcttaatcgc gtccttagtg gatttcaaaa cactcttgcg attgttgacc
    57901 aaggttccat cgatatcaaa aaaaacagct ttgacttcca tcctattcca atctcccctt
    57961 ttgtgataca atgattatac cacatttcag aaagagttag taaatcatgc ctaagaaaat
    58021 ccttgtttta catacgggtg gaactatttc catgcaggcc gatgcttctg gcgctgttgt
    58081 gacgagttca gataatccca tgaaccatgt gtccaaccca cttgaaggaa tccaagtcca
    58141 cgccttggac ttttttaatc ttccaagtcc ccatatcaaa cccaaacata tgctggtcct
    58201 ctaccagaaa attaaagagg aagcagataa ctacgatgga gtggtgatca cacacggaac
    58261 cgatacttta gaggaaacag cctatttcct tgataccatg gaagttcccc atatgcctat
    58321 cgttctaaca ggagccatgc gtagctccaa tgagctcggt agtgatggtg tttataatta
    58381 cctaagtgct ttacgagtgg ccagcgatga cagggctgct gacaaaggag ttttggtcgt
    58441 tatgaacgat gaaatccacg ctgccaagta tgtcaccaaa acacatacga ctaatgtcag
    58501 caccttccag actccaacac atggtcccct cggtctcatc atgaaacagg aaatcctcta
    58561 cttcaaaaca gctgaacctc gtgttcgctt tgaccttgat cacatacaag gtttagtccc
    58621 tatcatctcg gcttatgctg gtatgacaga tgagctgatt gatatgctgg atttagaaca
    58681 cttggacggt ttgattatcc aagccttcgg agctggtaat attcccaaag aaacggctca
    58741 aaaattagaa agccttctgc aaaaaggaat tccagtcgct ctggtatcac gatgctttaa
    58801 cggtattgcc gagcctgttt atgcctacca gggtgggggc gtacagttgc aaaaagcagg
    58861 cgttttcttt gttaaagaac tcaacgccca aaaagctcgc ttgaaactcc tcatcgccct
    58921 caatgccgga ctaacaggac aggctttgaa aaactatatg gaaggctaat actcttcgaa
    58981 aatctctgca aaccacgtca cgtcgcctta ccgtatgtat ggttactgac ttcgtcagtt
    59041 tcatccacaa cctcaaaaac atgttttgag ctgacttcgt cagtttcatc cacaacctca
    59101 aaaacatgtt ttgagctgac ttcgtcagtt tcatctgcaa cctcaaagca gtgctttgag
    59161 ctgacttcgt cagttctatc tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt
    59221 ctatctacaa cctcaaaaca gtgtgttgcg cagcctttaa ccagccgcct agtccgctct
    59281 atggtattca ttaagtcaac atctcttgtt taagagcaca aaatcaggaa atcttctcga
    59341 ttccctgatt ttttctattt acgttttcgt gttgagctac gttctgtcaa accatgaggt
    59401 aagagaactt cacgttcttc caactcttcc ttatgcataa tcttggtcaa catacgcata
    59461 ctaatggcac caaggtcata aagaggttgg gcaatcgttg tcaagtttgg acgggtaaag
    59521 cgtgagattt gtgaatcatc actagtaata atttcaaaat cttctggcac agaaacacct
    59581 ttatcagcca aaccgttcaa gactcctgct gccaactcat cacctgtcac aactgctgca
    59641 gttgcatttg atgaaatcaa acgctctgct aaggcgtaac catcatcata gctatattta
    59701 gattcaaata ccaaaccctc actataagtg attcctgctt ttttcaaggt ttccttgtag
    59761 ccaactaaac gaaccttacc attgatgtca tccactagcg gaccgctaac gaaagcaata
    59821 cgctcatttt ctttagcaag gtaactcact gcatcaattg ttgcttgctt atagtcaata
    59881 ttgacacttg gcaactggtg ctcaacatcg acagttcctg cgagaacaat cggagtacgt
    59941 gaacgcgaaa attctgagcg aattttatct gtcaagtgat aacccatata gataatgcca
    60001 tctacctgct ttgaaaagag ggtattgaca acagaaactt ctttctcgtt atcttcatcg
    60061 ctattagcta ggacaatatt gtacttgtac atttctgcaa tatcatcaat ccccttagcc
    60121 aaactcgaaa aataaccatt ggtaatattt ggaatcacga caccgacagt ggttgtcttt
    60181 ttacttgcaa gaccacgcgc aactgcattt ggacgataat ccaaacgatc aattacctct
    60241 agcacttttt tacgggtatt ctcttttaca tttttattgc cattgaccac acggctgacc
    60301 gtcgccatgg aaacacctgc ttcacgagcg acatcataaa tggttactgt atcatctgca
    60361 ttcattcctt ttcctgtcct ttctatctca cacattcttt tacaagtaga ggtgctgatt
    60421 gaagctctat atctacttac aaaagtgaag atgtgaaaat ttcgttttca tatttctact
    60481 tattccattc tatcactaat tgtaaacact ttcaagtgtt ttttgaagat tgattgaaaa
    60541 aatttcatag aaaacctagg tttagctcct tgctaccacc ttagactaaa caaaaaggag
    60601 gaaactaagc cctcctaaag ttatagtaaa atgaaataag aacaggataa atcgatcagg
    60661 acagtcaaat cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat
    60721 ctactatagg tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat
    60781 attatatcat cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc
    60841 agcatccagt tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta
    60901 aagctgatct gcgatttctt ggttggataa gccccgggcg attcccttta agacagcaat
    60961 ctcttgttct gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata
    61021 ctccttgcgt ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttcgttc
    61081 ctttaataca taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt
    61141 gaaggtcgtc accacaacca cctttgtttc aagcttttct gatcgtatcc actccaagac
    61201 ttcaagacct gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt
    61261 ttctaatagt tggattgctt cttgcccatt tttggtttga aggacagact ccacatctgg
    61321 ttgaagcgtg agcaattgac acatggcatc tcgcaacata ctttgatctt ctgcgactaa
    61381 tactttcatc ttagtttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt
    61441 tctgactgat tacacttact tctcctgaaa atggaaaaac acgatctcgg actgtatgga
    61501 gctcatctcc ttttagaaaa gcaaagccgc agccatcatc acttactgtt aaaatgagtt
    61561 ccttctctgt ccgttctaat tttaagtaag ctttagacgc tttggcatgt ttgatgatat
    61621 tggtcaccaa ctcaagcaaa atcatagaag ccattgaatc caattcttga gtaaggctag
    61681 cagtatctag ttggttatct gtttccacct caattccagc aatttctaac atctttttca
    61741 cagtctctag ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgaactt
    61801 cacacattga ttctttgcta atttgctgta tttctcttaa ttccttttcc acttgtggat
    61861 aagcctgcat ttgaaatagc tgcaaggcta gatctgtctt gacactaagc atagcaaagg
    61921 tatgtcccag actatcatgc aaatcctgac cgatacgatt gcgctcattt tctgctagca
    61981 atagatttat ctgggtattt tgcttgacct gagcttcttt caaatcctcg acaaggcgaa
    62041 ttcgaaccga gcccaaagtc atcaaatcga caaaagtaag aattacaagc atatagacta
    62101 gaaactcaac ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg acttgagcaa
    62161 ggagaaatgt ccatacatgt aaagacttta aactacgtac gccaaaatga taaattaaga
    62221 gattggaaag gaaaaagaaa aaccagatat aattcccagc aacaaaggcg gtattcccag
    62281 ctacatagac cagcatgagc ccccagaaaa gccaagatag gcgctggttc ttagttatta
    62341 aaacacccaa atacgtcact acaaatagaa tatcaaccaa taaatgccaa gcaggaagct
    62401 ccccaatcac tacaggtagg atgggaaaaa gcataaaaat caaactggtc caaaacatat
    62461 agtgtatgct tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat
    62521 agtcaaacca aataaaactg ctgaaaaaac aagtaaataa actgtggctg atagattgat
    62581 gtcaccctcg tttaagaagg tcttgagcaa ctccatcaat tgataggtcg ggagacactt
    62641 accaactgct tgcatccagt ctggaaataa agaaatgggc atccagagtc cgcctaaaac
    62701 agccaatcct agataaagaa gattgcccac aacagacatc agctgactag ttggtaagag
    62761 agtcaaggtc aagccaagtg ctacaaaagc aatactcccc acaatcagca aaatcgcagc
    62821 tccaacccat tttactagag gcatgtctac acctcttaca aaatgtccaa ctgtaaaaac
    62881 aaccagaatt gagaccaaat aatcaaccag catacttgtt atctttgata gataatattc
    62941 taccatattt acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg
    63001 taaaacagct ggaaatgaga ggatagttgt cgacatcatg gaaaaggcag tcatggagat
    63061 gaggtaatca cgcataaaat tcgcgagttc acctggtgtg tcctgataga taccagaaaa
    63121 aaataaatag aaagccgtcg gcatccctac cgacaataga taatagatta attgtcgttt
    63181 ggtcaataaa aattctatct tactaagtgc tatccatcgt ttcatcttag ttatctccct
    63241 tctgcgtctc ttcaaagatt gtattcaaca aactacgatt attaacttcg atttcttgca
    63301 tcctacatcc tgcttgggct aatagttccc agaaagcatc tgcttctcga gtgactactt
    63361 gtaaagaatc ttgctttagg gtccagtttt caaccaaatt tgactgctct acgacttcct
    63421 tgtaagctat aggaaggata aagtgctttt ctatttcctc gctacgcata gctagaggcg
    63481 tcgtatctcg aatcaactct cccttattta agagcaaaat gcggtctgca gtgtgttcca
    63541 cttcctcaat ataatgggac gaatagagaa tggtgactcc ttgcgctttt aactcctgga
    63601 caatttccca aaaacgttga cgagttgagg tatccatgga cgcagtaggc tcatctaaaa
    63661 aaacaatctt tggtcgccca attaaggtca agacgaaaga aaagagacgc ttttgaccgc
    63721 ctgataattt ttctgccaac tgttcttttt gttgcttgac aaactgcaat agttgatcaa
    63781 tttcctgatt gctcaaagga tttggataga tacgttgaaa gaaagcaatc aactctttaa
    63841 cttttaattt ctgaacgatt acattttctt gagggaggta agccctagta tagtctaact
    63901 tagaactcgt cactggtaaa ccttgaatag atacttgacc gcttgtgacc agtttatctc
    63961 caagcaaaca atctaagagt gttgtctttc cggcaccatt tggaccaatt aaggcaacac
    64021 attcaccatc gttgatttca aaggagatat tccgcaaaat ttccttgtct tttattttct
    64081 tactcaattt ctcaacttta accaccgtca tgagactctc ctttcaacca ctccattccc
    64141 ataaggaaaa cgacgaaaat cataaatcca aaccccaaag caccacgaat gaattggcga
    64201 agcaaggctt gatcaaacca atcagtaaac atttccacta accataccaa gagtgacagg
    64261 ccgataaaga aatagatgat ccctctcttc attcctcaaa ctcctttttc acatctctga
    64321 ctaatttcaa accttctcta acaagccaag acatgattcc aaagccagca aagaccttcc
    64381 aaggaaaatg gtagaaatcc tcatccaatc ccgaaaacat gagataggtc ataactcctg
    64441 ctactactaa actcattgca ataatcattt tatttctcat ctcttcttcc cccatttcat
    64501 actacaatta tagtctttta aaatcagagg aggcagaagc ttctgtcact agaaaatatg
    64561 acaaatgtca taaaaattct gttcaaaaca agcaagatac actatacaat aaaacacaat
    64621 cagaaaaatc taaggcaact tcctcaaaag agatatcaaa cccaattcac accataatgt
    64681 aaactaatac ttatttaaaa tcaaaaagag tagaaatttt tatcagacaa
//
LOCUS       DAVMAF010000003        56940 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMAF010000003 DAVMAF010000000
VERSION     DAVMAF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.003"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..1388)
                     /locus_tag="VYO97_000143"
     CDS             complement(<1..1388)
                     /locus_tag="VYO97_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV1753035.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKA"
     gene            complement(1678..1815)
                     /locus_tag="VYO97_000144"
     CDS             complement(1678..1815)
                     /locus_tag="VYO97_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1753036.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            complement(2017..2604)
                     /locus_tag="VYO97_000145"
     CDS             complement(2017..2604)
                     /locus_tag="VYO97_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1753037.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
     gene            complement(2697..2840)
                     /locus_tag="VYO97_000146"
     CDS             complement(2697..2840)
                     /locus_tag="VYO97_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753038.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            complement(2853..3233)
                     /locus_tag="VYO97_000147"
     CDS             complement(2853..3233)
                     /locus_tag="VYO97_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1753039.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(3226..3573)
                     /locus_tag="VYO97_000148"
                     /pseudo
     CDS             complement(3226..3573)
                     /locus_tag="VYO97_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3560..3811)
                     /locus_tag="VYO97_000149"
                     /pseudo
     CDS             complement(3560..3811)
                     /locus_tag="VYO97_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(3811..3981)
                     /locus_tag="VYO97_000150"
     CDS             complement(3811..3981)
                     /locus_tag="VYO97_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753040.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            complement(4168..4536)
                     /gene="rplL"
                     /locus_tag="VYO97_000151"
     CDS             complement(4168..4536)
                     /gene="rplL"
                     /locus_tag="VYO97_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1753041.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(4612..5112)
                     /locus_tag="VYO97_000152"
     CDS             complement(4612..5112)
                     /locus_tag="VYO97_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1753042.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(5151..5286)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(5362..6621)
                     /locus_tag="VYO97_000153"
     CDS             complement(5362..6621)
                     /locus_tag="VYO97_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1753043.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     KAVKEALTEMLQSGTTTFNDMYNPIGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(6923..8671)
                     /locus_tag="VYO97_000154"
     CDS             complement(6923..8671)
                     /locus_tag="VYO97_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753044.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLIRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(8673..10397)
                     /locus_tag="VYO97_000155"
     CDS             complement(8673..10397)
                     /locus_tag="VYO97_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753045.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVKLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDVLVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(10455..11393)
                     /gene="msrB"
                     /locus_tag="VYO97_000156"
     CDS             complement(10455..11393)
                     /gene="msrB"
                     /locus_tag="VYO97_000156"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1753046.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(11541..11612)
                     /locus_tag="VYO97_000157"
                     /pseudo
     CDS             complement(11541..11612)
                     /locus_tag="VYO97_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(11688..12557)
                     /locus_tag="VYO97_000158"
     CDS             complement(11688..12557)
                     /locus_tag="VYO97_000158"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1753047.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(12559..13845)
                     /locus_tag="VYO97_000159"
     CDS             complement(12559..13845)
                     /locus_tag="VYO97_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1753048.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(13996..14733)
                     /gene="mecA"
                     /locus_tag="VYO97_000160"
     CDS             complement(13996..14733)
                     /gene="mecA"
                     /locus_tag="VYO97_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1753049.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(14934..15968)
                     /locus_tag="VYO97_000161"
     CDS             complement(14934..15968)
                     /locus_tag="VYO97_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1753050.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYFFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(15996..17429)
                     /locus_tag="VYO97_000162"
     CDS             complement(15996..17429)
                     /locus_tag="VYO97_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1753051.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLVHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(17445..18431)
                     /locus_tag="VYO97_000163"
     CDS             complement(17445..18431)
                     /locus_tag="VYO97_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1753052.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(18433..19536)
                     /locus_tag="VYO97_000164"
     CDS             complement(18433..19536)
                     /locus_tag="VYO97_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1753053.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSTSNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(19526..20371)
                     /locus_tag="VYO97_000165"
     CDS             complement(19526..20371)
                     /locus_tag="VYO97_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1753054.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(20650..21924)
                     /gene="psr"
                     /locus_tag="VYO97_000166"
     CDS             complement(20650..21924)
                     /gene="psr"
                     /locus_tag="VYO97_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1753055.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(21921..22769)
                     /gene="pheA"
                     /locus_tag="VYO97_000167"
     CDS             complement(21921..22769)
                     /gene="pheA"
                     /locus_tag="VYO97_000167"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1753056.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAENPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(22766..23242)
                     /locus_tag="VYO97_000168"
     CDS             complement(22766..23242)
                     /locus_tag="VYO97_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1753057.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(23235..24518)
                     /gene="aroA"
                     /locus_tag="VYO97_000169"
     CDS             complement(23235..24518)
                     /gene="aroA"
                     /locus_tag="VYO97_000169"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1753058.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDSKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(24610..24948)
                     /locus_tag="VYO97_000170"
     CDS             complement(24610..24948)
                     /locus_tag="VYO97_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1753059.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(24959..26062)
                     /locus_tag="VYO97_000171"
     CDS             complement(24959..26062)
                     /locus_tag="VYO97_000171"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1753060.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(26072..27238)
                     /gene="aroC"
                     /locus_tag="VYO97_000172"
     CDS             complement(26072..27238)
                     /gene="aroC"
                     /locus_tag="VYO97_000172"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1753061.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(27248..28315)
                     /locus_tag="VYO97_000173"
     CDS             complement(27248..28315)
                     /locus_tag="VYO97_000173"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1753062.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPASITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(28334..29188)
                     /locus_tag="VYO97_000174"
     CDS             complement(28334..29188)
                     /locus_tag="VYO97_000174"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1753063.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(29178..29855)
                     /locus_tag="VYO97_000175"
     CDS             complement(29178..29855)
                     /locus_tag="VYO97_000175"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1753064.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(29852..31015)
                     /locus_tag="VYO97_000176"
     CDS             complement(29852..31015)
                     /locus_tag="VYO97_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1753065.1"
                     /translation="MDRIRVSKRVEKKLAKGLVLLESSDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(31699..33123)
                     /locus_tag="VYO97_000177"
     CDS             complement(31699..33123)
                     /locus_tag="VYO97_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753066.1"
                     /translation="MPVYFSFVVAILLVIAFSTSLTAFYNVFYESKDLASYRPYAFKE
                     SEIIIAKGLSVLFPALTGIVPILAYFLALYISLAPSLWLGLPLMLLSLTLLFVSVALV
                     MVVAVHFLAQTTAFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGSIVDKVRDIPFLL
                     YPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYDVILLNSEEKVKK
                     ERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYLMMISLISKIRDS
                     QIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERKNVDFLKSLPFDFARYVK
                     VKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIFSCHHYFKDVKNL
                     STNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSPILAINLGVGALI
                     VLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(33314..34063)
                     /locus_tag="VYO97_000178"
     CDS             complement(33314..34063)
                     /locus_tag="VYO97_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753067.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQDLSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSSSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            34242..35696
                     /locus_tag="VYO97_000179"
     CDS             34242..35696
                     /locus_tag="VYO97_000179"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1753068.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(35786..38404)
                     /gene="alaS"
                     /locus_tag="VYO97_000180"
     CDS             complement(35786..38404)
                     /gene="alaS"
                     /locus_tag="VYO97_000180"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1753069.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(38426..38911)
                     /locus_tag="VYO97_000181"
     CDS             complement(38426..38911)
                     /locus_tag="VYO97_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1753070.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(39186..39425)
                     /locus_tag="VYO97_000182"
     CDS             complement(39186..39425)
                     /locus_tag="VYO97_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753071.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(39678..40748)
                     /locus_tag="VYO97_000183"
     CDS             complement(39678..40748)
                     /locus_tag="VYO97_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1753072.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(40745..41518)
                     /locus_tag="VYO97_000184"
     CDS             complement(40745..41518)
                     /locus_tag="VYO97_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753073.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(41515..42321)
                     /locus_tag="VYO97_000185"
     CDS             complement(41515..42321)
                     /locus_tag="VYO97_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753074.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(42302..43459)
                     /locus_tag="VYO97_000186"
     CDS             complement(42302..43459)
                     /locus_tag="VYO97_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753075.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(43620..44525)
                     /gene="murB"
                     /locus_tag="VYO97_000187"
     CDS             complement(43620..44525)
                     /gene="murB"
                     /locus_tag="VYO97_000187"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1753076.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(44637..45389)
                     /locus_tag="VYO97_000188"
     CDS             complement(44637..45389)
                     /locus_tag="VYO97_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1753077.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSTVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLSQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(45399..46130)
                     /gene="budA"
                     /locus_tag="VYO97_000189"
     CDS             complement(45399..46130)
                     /gene="budA"
                     /locus_tag="VYO97_000189"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1753078.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            46272..47543
                     /locus_tag="VYO97_000190"
     CDS             46272..47543
                     /locus_tag="VYO97_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1753079.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(47563..48378)
                     /locus_tag="VYO97_000191"
     CDS             complement(47563..48378)
                     /locus_tag="VYO97_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1753080.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(48535..49188)
                     /gene="phoU"
                     /locus_tag="VYO97_000192"
     CDS             complement(48535..49188)
                     /gene="phoU"
                     /locus_tag="VYO97_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1753081.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(49200..49958)
                     /gene="pstB"
                     /locus_tag="VYO97_000193"
     CDS             complement(49200..49958)
                     /gene="pstB"
                     /locus_tag="VYO97_000193"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1753082.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(49971..50774)
                     /gene="pstB"
                     /locus_tag="VYO97_000194"
     CDS             complement(49971..50774)
                     /gene="pstB"
                     /locus_tag="VYO97_000194"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1753083.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(50785..51669)
                     /gene="pstA"
                     /locus_tag="VYO97_000195"
     CDS             complement(50785..51669)
                     /gene="pstA"
                     /locus_tag="VYO97_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1753084.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(51659..52576)
                     /gene="pstC"
                     /locus_tag="VYO97_000196"
     CDS             complement(51659..52576)
                     /gene="pstC"
                     /locus_tag="VYO97_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1753085.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(52576..53454)
                     /gene="pstS"
                     /locus_tag="VYO97_000197"
     CDS             complement(52576..53454)
                     /gene="pstS"
                     /locus_tag="VYO97_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1753086.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(54028..55332)
                     /locus_tag="VYO97_000198"
     CDS             complement(54028..55332)
                     /locus_tag="VYO97_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1753087.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(55333..56106)
                     /locus_tag="VYO97_000199"
     CDS             complement(55333..56106)
                     /locus_tag="VYO97_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1753088.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(56096..56374)
                     /locus_tag="VYO97_000200"
     CDS             complement(56096..56374)
                     /locus_tag="VYO97_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1753089.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(56374..56775)
                     /locus_tag="VYO97_000201"
     CDS             complement(56374..56775)
                     /locus_tag="VYO97_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1753090.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT        16968 a        12841 c        10581 g        16550 t
ORIGIN      
        1 aaagctttta taataaaatc ttgtatatct tgctcactct cgatagttct aatctcatgc
       61 atgggtttta aaactttttt cctggaaata ttctcaatct gtggacattt agaatctaga
      121 tatgacaata aactttctac ataatctata tgttctcttg tataacccaa agactcaaat
      181 agtttttttc cttctatcct ggtttgactt acatagttgt atgtcaaatc cgatgtagtt
      241 actagtggca tgtataaata atgagctatt tgtctaatac cataccaatc tatctcactg
      301 ggaagtgttt ctcgccatgc tctaaaacca ggggctgcaa ctttatgtac aactttttca
      361 tcattcgaaa agacagcctg ttcccagtct attatactaa tctcatcttc atccttaacc
      421 aagatatttc ctaaatgtaa atcttgatga tatacatttt cagaatgaaa cttattcgtt
      481 aaatcgatga gtttttctac tatctttgaa actctcaata gataatcttt ggtcttatca
      541 acaacttcat ataaaggaaa attattggta acccatctat ttagtggaac gcccttcata
      601 tgttcaattc ctaagaaggt gtgctcccag atcttaccgt accagtatat tttaggcatc
      661 tcactccatt catttagaat ttttagtgct ttgcactccg aagctaatct ctctgaagaa
      721 taagtaccat caaatcctag acctgtatac ggtctagcct cttttaaaat tatttttttc
      781 ccatcttctt ttagtctagc attatatatc ccaccactgt ttgaaaatct aattgcatta
      841 tctataataa agggaaagtc tccctgtttt ttatctttct tgtcaagcca tttattcaaa
      901 aagtcagggg gcactatacc ttttggaatt ttaaatactg gtaaacgttc atctttaaca
      961 acttcatcgc caacaattaa ttcatcaata acaaccttct tttcatcatc ccttgacggc
     1021 ctaaacacac catacctcag atatattggt gcttcatccc aacgtttatc gcttaaaata
     1081 tatggcccat tatattgctt taaggcactt tctaaccttt gcaaaaccga ctctaattca
     1141 ttttgatttg gataacatgt aataaattta ccagaaaatc ctcgactaac caatttcccg
     1201 tttcgcatga taaatttgtc ttctgtacta agatgtttaa atggaattcg catttcatgg
     1261 caaatttttg ctacatcttg taacaattca tgtgaactgt tatactctga actaatgtgt
     1321 attttccacc cttgtctttc aacaaatttt ccaatagggt attgataaac ccactcatca
     1381 ttattcatta cttcgtgcca attaaaaggc agacttactt agtactttat gctagtatct
     1441 gtactataat cattattagt gaaaaagaaa ggatgctcca aattgaaatt ataatccata
     1501 acaaaatctc caagaaattc tatcaaactt aatatatcta tagctagaca gacttattaa
     1561 aataaaaagg gagaatcctt tggattcccc ccatataagc actgacattc caacgtgcac
     1621 atattggaac gagatccata actccagaga atccctaaag tttacaattt aaatgaatta
     1681 acaattttcc caactaaaag cactccagtt accgcaacgg tttgtactga atgtactaaa
     1741 tcgcattcca tcaacttcat ctgtttcgtc aacttgaaca gatactaatt gaagatttaa
     1801 tacttcttct gccatagcta actcctccta tttaaatttt tgggattaag tgctttatcc
     1861 accttcatta tactctctcc agcagtaaaa tgcaagcaat gatacaatgt tgtcacgtag
     1921 aaaataatgt ttccgtaact tttcaaagta acttccatct ctctcccaaa actggaagtt
     1981 agttttagaa gttacctaaa aatcaggtta cctattttaa aaaagcagca aactataaac
     2041 tagtaggctc cacaccaaat gtagccccat actgccccat aagtccaatt tgtagcgtac
     2101 aagccctaaa aacatcccca gcgatacata caaacaccaa gctataatgg ttcctggatg
     2161 atgtgctaag gcaaataaaa cacttgtcaa agcaactctg atatctaatt ttctgaccaa
     2221 attccataaa atttctcgat acagaaattc ttcaaccata ctcgcattga ttaagaacaa
     2281 taaaaatgaa aaccaaggaa tttgatgttg aaggccaatt aagtttgctt gattcgtgct
     2341 tccttgagca tgaatcagac taaaacatag acttataatc agtaggctaa caaattcaac
     2401 accaagccat ttcatcctag atttcatatt gaccttatgc gcttgtttgc gttggccata
     2461 catccataaa aaagaaatga gtgacgaacc atagagaatc tgtagtatag ttaactcacc
     2521 gatacaaaga aatttcaata agtatagaga taccaatagg acatttactt gttggaatat
     2581 ataaactgga attattcttt tcatagttac ctccgaaata aatcttcata atctaaatct
     2641 aatacctgca caatcctttc tacccatgga ctttgaggca ttcattgttc catcttctag
     2701 tggcgaatct tttgatacaa acgattcaat tcacttggat agtgaaactc tcccgcaaac
     2761 atttttctgg ttaactcaat ccagctgata tttctttcag ccaaaataat ggacaagttc
     2821 tcccaaaatc gttcagccat attgcttctc ctttagttag ataaataatg tgtttgcgcc
     2881 atgtaaatca attgtttcgt atctcttggc aatagagctc tagcctcttc caaattcaga
     2941 cttggataaa ctcgcttatt tgaaaccgca agaggaagtc tgatggttag ttcaggattt
     3001 tttaaaatta tctcaacgaa atccgttaat cttagattgt cacggttctt aaatcgtaat
     3061 aaattgggag ataaaaactc aaaacaatct gaagaatagc tcatcatctc aattaatttg
     3121 tcctttgtca tttcagaaac tgaatgacaa gatacctcta tgccatagtt ttggaagaaa
     3181 tctaaaagaa gttgatttct ttgtctattt ttacttagat agagatcaat catgggagac
     3241 ctcccaaaga ttcggttcca tttgatattc tgagacgatt aaggaatcta ataaatttgc
     3301 gaagttaatc ggtttcttgt cttcatcata agcttttaca gttacttggg ttgtaagtat
     3361 tccctctttt ccctcggctc gatagccttg tccatataaa acaaaaacga gattttgatg
     3421 atcatctaca aaggcatcaa ccccattctt tatgtcttga ctttcaagga attccataac
     3481 gttttgaaga taggactcgt aaaatagtgg gtagttgtgt tttttatggt aatcatctaa
     3541 aaatgttact tcaaactcac atggagagta attttgactt tgaacagcct aaaagtgcca
     3601 tcaaatttga attggaataa atcaaataaa tagccccatc ctcatcaatc caacctttgc
     3661 tcaaagacaa ctccaaccga tcttttaaaa ctgagtaaac caccttaacc tccagtttca
     3721 tattcttata ccgttcactc tcaaataaaa gtttggggag cttataataa cgctctgatg
     3781 tctgatattg attagcggta atacgcttca ttattgtccc tccaagacta aaattccaac
     3841 atttccaaat tcatcaaatc ggattaaacc tacttgttcc atttcatcaa ctaactgagt
     3901 tgcttttaca atatcaactc ccatgatagt catgagatga ctcttcacga attgacgtga
     3961 tgactgtcct tccttttgca taattacctc cgaaacacaa aaaaaggggt agacaatcta
     4021 gtgtctaccc ccgaaagttt attaaaacaa aaatcctgcc aaagaatttt tggcaggaaa
     4081 ccaaatcaat ttatcagttt ctatcaatcg cttatcgctc tcaaagactg gtaaataggg
     4141 attccgcaat caaattgcga tactctatta tttaagagta actgaagctc cagcttcttc
     4201 caatttagct ttgatttctt cagcttctgc agttgcaacg ccttctttaa caagtgctgg
     4261 tgcaccgtca acaagttctt tagcttcttt aagaccaaga ccagtgattt cacgtacaac
     4321 tttgataacg ccaacttttt tgtcgcctgc agatgtcaat tcaacgtcga atgaatcttt
     4381 agcagcacca gcatcagctg catcagctgc agcaacagct acaggagcag ctgcagttac
     4441 accaaattct tcttcgatag cttttacaag gtcgttcaat tcaaggattg aagcttcttt
     4501 aatttcagca ataatgtttt caatgttcaa tgccattgtt atttcctcca aataagtttt
     4561 aaattttata atagtttttt tcgtagctag gctacgctgt gtagcttaag attaagccgc
     4621 gtcttctttg ctttctgcaa ccgctttgac tgcaagagca acgttgcgca ctggcgcttg
     4681 aagtacagaa aggagcatag aaagaagtcc ttcgcggttt ggaagagttg caagtgcaag
     4741 aatctcttct ttagatgcga cagcgccttc gattgcacca cctttaattt caagtgcttc
     4801 agcgttttta gaaaagtcgt tcaagatttt cgctggtgcg ataacatctt cattagaaaa
     4861 tgctactgca gatggtccaa caaatacaga tgcaagatct tcaagaccag ctttttcagc
     4921 tgcacgacgc aagattgagt ttttaataac tttatactca acttcgcttc cacgaagctc
     4981 acgacgaaga actgtatctt gctcaactgt caaaccacga gcgtctacaa cgacgataga
     5041 tgcagcagct ttcatttttt cagctactac gtcaactagt tccgcttttt tagcaataat
     5101 tgcttcactc attagtgtgt tcacctccgt aattattttg cttggggaat ttttcaaaaa
     5161 gaaaaacgcg cccaaaccta gacacgaaag tacaatacgc ttctttttac atgatacgtc
     5221 ttgtcctcgg taggatattt atgagtcgag ctcccctact gtcttaggca gtttttttaa
     5281 accgtatata agtatagcat agtcaaaaaa agaatgcaag atttttgcaa acttttttta
     5341 aaatttttcg taatttttct tttaaagttc tactgtcagg acttgacctt gcttaacaac
     5401 ttgttctccg gcgatataaa catcatctac atcactagat ttaactgcat aaaccaggtg
     5461 agacagcata ttttcctgag gttggagatg aattttccct tgtggttgaa tgaccagaaa
     5521 atctgcttgc ttgccgactt ccagacttcc tatctgattt tccattccaa ggaccttagc
     5581 cccttcgatt gtcagtacct tgagagctgt ttcgattgga aactggctgg catccccact
     5641 tttcatcttc tgaagaagag ctgcagtcct tccttcctca aacatatcta gattgttatt
     5701 ggaagcaacc gagtcagtcg caattccgac tgctactccc gctttttgga gctggataat
     5761 tggagcaatt cctgatgcca gtttgaggtt actgatagga ttgtgggcga tagccacttg
     5821 agaagatgcc aagcgttcaa tttctctctc gtttaattcg accccgtgag caaatacgga
     5881 cggatgatct aaataaccca gttcttcaag aaaagcaagg gggcgtttgc cgtatcgttt
     5941 gaggataatt cctgactcct ccttggtctc cgccacatgg acatggagcg gaatatttag
     6001 ctcttttgcc atttccaaac tcgcttccag caagtctcta ctgcagctat acggagaatg
     6061 aggtgctacc ataaccttga aatttggatt tttatatttt aagatttcat ctatgatgga
     6121 tcgagttctg cttatagtct cagcagttgt ttctgtctct gaagaaaaga gagtcggaga
     6181 aaaataacaa cgcatcttgg aagttttcac cacctgataa atttgctgga tatccacacc
     6241 aatgggatta tacatatcgt taaaggttgt tgttcctgac tggagcatct ctgtcaaggc
     6301 ttctttgacc gccttggtag tcatgtcggg agtaaactca gattctgctg gccagatata
     6361 gtcattgagc cattcatgga gattgctgtc atctcggatc cctctcagac ctgtcattgc
     6421 agaatgggtg tgacaattga ccaaaccagg cataatccaa gctccctgat agtctataat
     6481 ctgctcagct tgttctaaaa acgctggctt atcttgaccg acatagacga tttgagaatc
     6541 cttgactgct aagattccat caagataaac atggaaattt tgatcacaag tcacgatatt
     6601 tacatgctga aagactttca ttatagactc cttttttaaa agacaagttg tactctcttc
     6661 aaaccacttc agattcactt tgccgtaggt gtggttactg acttcgtcag ttctatctgc
     6721 aacctcaaag cagtgttttg agctgacttc gtcagtctta tctacaacct caaagctgtg
     6781 ctttgagcaa cctacggcta gcttcctagt ttgctctttg attttcattg agtattacag
     6841 taaacaattt ttctagctat tgtaacaaaa aagtcactcg aaggcaactt ttttcgtcca
     6901 taaactttca aaagagaatg gcttattcag aactaaaagc cgcagctttt tgcatttggt
     6961 aatacttacc ctttctatcc atgagttctt gatggttacc atattcaaca atatcaccat
     7021 ctactaagac aagaattaaa tccgcatcct gaatggttga caaacggtga gcaatgatga
     7081 agcttgtgcg gcccttcatg agttttgcaa aggcatcctg taccagcact tctgtccgtg
     7141 tatcaatgga agaagttgcc tcgtctaaga taagaatctt tggaatagcc agaaagactc
     7201 gggctatggt caagagctga gcttggccga cagagagaga ttctccagca ttttccaact
     7261 tggtatcgta tccctgtggc aactgttgga tgaaaaagtc tgcattagct gctttggcag
     7321 cagcaattac ttgctctcga ctggcttcag gattgccaaa ggcaatatta tcatgaatgg
     7381 tcccttgtgt gagccaggtt tcttgaagca ccatgccaaa ctgctgtctc aatgatgctc
     7441 gtgtataatc ataaatggat tgcccatcca gcaagatatc tcccgagcta atgggataaa
     7501 aacgtataag gagattgata agagttgatt ttccagcacc tgtcggacca acgatggcta
     7561 ccttactacc agctggaata tcgatagaca agtccttaat caaaattttt tcaggatggt
     7621 agccaaaaga gacatgttta aaggaaatag ctcccttaac ttggtcactg gtcaagactt
     7681 ccttacctgt ttcagccact tcagggctat ctaagactcc atagatacgc tctacgcaag
     7741 ccagagcact ttgcaactca gctagcactg aagaaatatc gttaaagggc ttggtgtatt
     7801 gctgaacata gttcaaaaaa gtcactaaac gaccgacggt caaggttgaa cccatcatga
     7861 tacgataagc tcctactcca gctaaaaggg cataaatgag tgcatttaca aagcgagtcg
     7921 aaggattgac cgttgaagaa taaaagatgg ctgactgaga atagcctgag tagttgtcat
     7981 gagcctcacg caatctttgg ataaactctg tttgagcatt gaaggactgg attatagtct
     8041 gctggctaag cgattcttca atcaactggg tctgaatccc cctcgtctct gtttgcttct
     8101 ggaagagatg ataggatctc ttggcaataa agcgtgaaat caccatggac agtggcgtca
     8161 acagcaagac taagagagtc atgaggagat gaatttggag catggctaga atactaacca
     8221 aaatcatcaa aacaccaatg aaaaattggt taaaaatcat agtcaagcca gctgccaact
     8281 gttcgatgtc cgtggttaca cgactgacca tctctccatt gccttgccga tccgcaaaag
     8341 caatcggtaa ccgatggagt ttatggatga ttcgctctcg taaatctctg gtataagaga
     8401 agattagacg attatagagg agaggattgg cccattgtac cagagtattt cctattacca
     8461 ccaagagcat ctgtagaaaa atctgccaaa aaactggtga tgaaccagcc actaggactt
     8521 ggtcaatgac ctgcccaatc agaataggta ggtaaattga taagccaact tgggcaatag
     8581 ttcctagaaa ggctaggaaa aggaggaagg gatggtttgc taaatctacg gctaaacgtt
     8641 tgagcgtctg gtttacagtt tgtcgtttca ttctagtcct cctttccatg ttgggatgca
     8701 ttgatttcac gatagacttg gctggatttc atcaagtcat cgtgcttgcc aacagctagc
     8761 aactcacctt tttccaagag gagaatctgg tccgccatct gtaaagttga ggttcgttga
     8821 gagatcaaaa ttaagctcgt gtttggaaaa ttttctctaa tagctttcaa gagcttggac
     8881 tctgtaatgg tatccagtgc cgaggttgca tcatctagga tgagaaacgg agcttggcgc
     8941 aagactgctc gggcgataga caacctttgt ttttgtccac ctgagaaatt tcgcccccct
     9001 gcctcaacta ggacatccaa gagtccttcc ttttcactga caaaatcctt agcttgcgca
     9061 atctccaagg cctgccagag tttctggtca gatacttctt gattgaaacc tagagtcaag
     9121 ttggaacgaa tagttccctt aaagagttcg accttttgag gtacataggc aatccaagac
     9181 cgccactgct ccaaattaag aggactacgt ccattttgat aaaggtcaat gttccccttg
     9241 tctactggat aaagtccaag taagagtttc accaagcttg atttaccaga accagttccc
     9301 ccgatgatac ctagaatttg tccttgagtc atatcaaagg aaatgtatct cagagaaggc
     9361 tgggccgcat caggataggt aaaggtcaat tcttggactt gtaaaacctt atctctggta
     9421 gcttgctttt gttctaactc tgaatggata tcctctggag cctcaacaaa gacttcctcg
     9481 attcgcttgg ctgagatata ggactggttg agggaattga tcaacatggc tagcttgacc
     9541 aattccacca aaatctgtaa gaggtaattg ataagagcaa tgagagcacc ttgactgagc
     9601 actcctcctt gaattgaaat atagccttgc cagataataa cgagaagagt tccattgaca
     9661 atcagatagg tcagaggtgt taataaacta gaccagaaac ctgtcttttc ttgtaatcta
     9721 gcataaactt ggttaagggt ttgaaaaatc tgtaactctc gtttttcttg accaaaagca
     9781 cgaataaccc gcatcccttg caattgctgg cgcgtttcct gaaccagttg gtccgttttc
     9841 tttctgagac tactgtagag aggattgacc aatcgagata accctacaat gacaatggtc
     9901 aaaatggcaa ccatgactaa gaaccagaaa gtcaactcag ctgagattcg ataagccata
     9961 aaaatggcac caaaaacgat aatgggcgct cgcaaaaaga gacgcaggaa ttgattgata
    10021 ccagtctgaa tctggtaggt atccgaagtc aagcgagtga ccaaactaga agttgtcaga
    10081 cggtctctgc tatccttggg caaggaaaga atatgacgat aaagatcgtt tgtcaattcc
    10141 ttagcaaagc ctactgctgc ctttgctgag taaaattgag ctatcaaggc cactaaaacg
    10201 ccaattactg caaagataag gagcaggcca atctgcatcc agagatgacc ttgatctccc
    10261 tgaggtaaag attggtcaac aatcccagca atcaccatgg gaaccaagag ctcaaaaaca
    10321 gcttctaaca gcttgaacaa gggggctaaa attgattcct tgatgtaggg tttgaagtaa
    10381 gataataggt gtttcataaa tcccttctat tcatattcta gaaatgaaga aagtgggaag
    10441 ccccactctc tgttttattt gtttaagtaa ggcaatagat agccatatcc tgctttttcc
    10501 atctcatcct tggccacaaa gcgtaaagaa gcagaattga tacagtaacg gaggccgcct
    10561 aactcccgcg gtccatctgt gaaaacatga cccaagtgag cactgcctga acgagaacga
    10621 acttcaattc gctccattcc atggctcaga tccttgtaat aatgaatcaa ctctttggaa
    10681 atcggacggc taaaacttgg ccaaccacaa cctgaagcaa acttatcctt ggcaaaaaag
    10741 agtggctcac ctgtcgtaat atctacataa atcccctctt caaaggtttg gtcataggca
    10801 ttggtaaacg gagcctctgt agcagcttct tgtgtgacac gataagactc ttcagataga
    10861 ctggccttca acacctcttg actaggcttt tcatagtttg ctgcatcaat caatggctta
    10921 tcagcatcgg tcacatcgat atgacagtaa cctgaaggat tcttcctgag ataatcttgg
    10981 tggtagtctt cagccagaat gtagtggcgt aattgctcca cttctactgc aatctttcga
    11041 cccagcatgc gttcctgctc ctgtaccact gtgtagatag ctggcaaatc tgcttcatcc
    11101 tgataataaa tcccagttcg atattggcga ccacggtcat tcccttgttg attgatagat
    11161 agaggatcga taactcggaa ataataaagt aaaatctctc tgagtgacac ttccttctca
    11221 tcgtaaatca cttggaccgt ttctgcatgg tctgtttcct tgatcaactg gtaattggtc
    11281 gtttcgactt gaccattagc gtagccaaca ctggtttcta gcactccaga aatgcgtgaa
    11341 aaatattcct ctaggcccca aaaacaacca cctgctagat aaatttctgc catctttatt
    11401 tctcctttat cacgttttta actaatactc ttcgaaaatc tcttcaaacc acgtcagcgt
    11461 caccttaccg tatatatgtt actgacttcg tcagttctat ctgcaacctc aaaacagtgt
    11521 cttgagctga cttcttcagt tctatccaca acctcaaaac actgttttga gcagcctgcg
    11581 gctagcttcc tagtttgctc tttgattttc attgagtata aacttctttt cacaaaaagg
    11641 atatgaaacc ctctggtcaa gagtttcccc atcctatctt ctattcttta ttttgcttct
    11701 acacggacac cttgggtatc aactctcaag tcatgcagtt ttcctttgaa aggttgcttt
    11761 tccaattctg ccttaattgt tggcatcttg tcatgagaag ccagaaccat aactgtcggc
    11821 ccagcaccag aaaggtaggt tgcataggcc ccattttctt tggtcacttg cttaatcatc
    11881 gcaaattctc ttaccaagtc ctgacgatag cgctcatgga agaggtctcc ctcgattgct
    11941 tgcccagcgg tcaccatgtc tcctgccaac aaggcagcaa ccgctacatt ggcgatagaa
    12001 cttgcagcaa cagcttcctt ataagacaat tttttaggca agacactacg gctgtcgcga
    12061 gtacgtaatt catagtttgg aatgtaagct agaaaatcac actctggaaa gtctgctacg
    12121 atagcagaga cttgcccttc aacagaactt gcaataacga gattaccata aatggctgga
    12181 gccacattgt caggatgccc ttcaatcttg gtcgctaact gcaatttttc atggtctgat
    12241 aagttgagtt gacccagttg gttggctagt tcaatcccag caacgataac cgagctggaa
    12301 gaacccaaac cgcgcgccaa agggacatca ctggtcattt tcaagcgtct tggttgcaag
    12361 tctggtacaa tttgcaaagc gattttgagc aagagattac gctcatcatg tggaatccat
    12421 ttgccaatct ggtgttcaat cagccactca tctcgttctt cgcagacctc aatttgaaga
    12481 tacttggtta cagctacacc gaccgagtca aaacctggcc cgatattggc actggttgca
    12541 ggtacaataa tcttcatctt attctcctag caccttgaag gtattcaaga ggtcgaattc
    12601 tgaaaccttc ttcaattcag ctgagacatt ttcaagctgg gctttattaa tcttgtgtgt
    12661 gatgataacg acacgcgcct tgtcaccctc tttgccatct tgaaggattt gcttaaagga
    12721 aatatcttga gcattgaaga tttcagccaa cttcaagacc tgaccttttg agtctggagc
    12781 caagattgag aaatagtagt ttgctttgac atcttcagga tttgccaaga ccaagtcacg
    12841 gctatattcg ttgaagtctt tgccaatggt accatcattc aaacgacgaa cgatacggac
    12901 aatatcagct acaacacttg ttgcagttgg tttttgaccc gcacctggtc cgtagtacat
    12961 agactcacca ataccgatag attctacaaa gacagcgttc attacgccat tcacactagc
    13021 aagtgggtgc gctttaggta ggaaggttgg agtcacttct gcagcaatac ctgaagaagt
    13081 ttcctcaata gaaccaacca atttcactac gtaaccaagc tcttgagcta cagctacgtc
    13141 ttctggtgtg atattgcgga ttcccttgtg ggctacatca tcaaaggcaa tcttcatgcc
    13201 aaaggcaaat tggctcaaaa taaccatctt gtaggctgca tcaatcccat ctacgtcatt
    13261 cgtcggatcg ctttctgcaa atcctagacg ttgtgcttcc gcaagagcat catcgtaaga
    13321 ccagccttct tccaccatct tggtcaccat gaagttggaa gttccgttga ctactccaag
    13381 cacgcgcgta attttatcag aagccaagga atttgctaaa gtacgaagaa ttggaatccc
    13441 accagcaact gctgcttcgt agtaaagtgc taccttgtta gcttgagcga tttctagcaa
    13501 ttctgcgcca tggacagcta aaaggtcctt gttagcagta acaacgtgtt ttccagcttc
    13561 caaggcacga gtgataaagg ttttagcagg ctcaatacgc cccatcaatt ccactacgat
    13621 agtaatatcc tggtctgata aaatatcatc cacattggtt acaaagttaa agtcattccc
    13681 tgctgcaagc aagcgatttt tttcatcttc atccttgacc aataccttag caactttgat
    13741 atctgaatgt gctgattgat tgatttttcc tccattttcc tttaggagga aaggcacacc
    13801 acttgcaacg gtaccaaatc ctagtaaagc aattttaact gtcatcttta tctcctcgaa
    13861 attttctaat atagccatta taacagaatt ttgtgaaaat tcctattata gtaaatcact
    13921 atttcagtat aaaaagaaaa aacgaatcag acgattcgct cttcttaaaa tctgaaaata
    13981 gctttccaga aaggattagc cgattttttg cagattgagc actgcatcgt gactcatcaa
    14041 gacttgacca tactcttgta agactgagcg actaatatca ctatcgtctg caaactcgcg
    14101 catacgggcc aacagccaag ctggatatgg gcttggatga ttttcaatat ccactaaaat
    14161 ggtcaaataa tagcgctcgt tcattttgta gagttcagaa gtttccattt caaaagtcac
    14221 tgtcttggca aaagctacca agtcagccaa cttagcaaaa gaaaggatgt agtagatgta
    14281 aggttctttc ttactctcag cttcttgttc agcctgctct tgctcttctt ccttggcttc
    14341 aacttgctca agagattgaa tggcttcgat atcatccttg gttttgtctg cgatgctttt
    14401 ttccagggtt ttgataaatt catctggaga catttgagcc aattcttcca tatctggcaa
    14461 atccgataag tcttcaaaat ctagattttg gtcaatcttt gacttggtca caaagacatc
    14521 taccttatca ggttttggag tcacacggaa gctcaacatg cctgtatcca gaaagctatc
    14581 aggcatctct agctcatcca agatagcata aaagaactct tctgtttttt cttgaggaac
    14641 gagaaagtca gcaatctcca ttccacgatc catcaaatcc tctaaagaca tcgtgatttt
    14701 taaagttgta tcactaattt gtttcatttt cattgctagt aacctcatac tttcagttct
    14761 atctattata ctagattttt acgattttat caaaagaagg ctcctctata cggatagatt
    14821 ttccctaggg taacttctgt aggagactcc aaaagaaaat ttctgcagac agatagaaaa
    14881 agccttcaaa atcagctctt agccgatttt gaagacctca tacatcagaa tacttataat
    14941 ttaaaggttg ctacaccgag gatagaacga tttaagtttc tgagaatttg aagactttgc
    15001 tcaaatttct tataacgagt cactccgtac tcttcaacaa gaaggactgt atctctttcc
    15061 aaaagagatg atacatcctg taaatctaca aaatgcattc cttttaaagc ttcttgactc
    15121 tgtttcaatt tatctaggat agctttattt gagctaacga tggtcaattc ctgtccagta
    15181 tttttgtatg acaaaacatc tgctaggtta gcaattgttg taatctctgt tacaaaatca
    15241 atttgatact gagaaaaatc acctactcta ttgattgttg gattaaagaa ataaactaac
    15301 acatttccca tcacaaccaa aatcacacaa accactccaa taacaactaa acgaagaatc
    15361 agatttttca catttaagcc aagcgctgtt tcaccatttg cgttcaattc tttagagttg
    15421 atggtttcca gtttttcaat ttttacattt gcataggcat gtttaaattt ctcaatcaac
    15481 ccatcaattt ttttctctaa caagtcattg gcatctttac ttgatgtcaa aattttcaca
    15541 ccaacccctg catcgtcaat catatagtag acggtcaatt ttttccacca atagtcattc
    15601 gttgaatttt tcaaggttgt ttctgtcgtg tctaattcac tggcaatttt tttcaactca
    15661 ctgggttcta catcattgaa aagataagct ccattcaaat taccatcaat caatttccca
    15721 taaaaatcac tataaccacc aatttgatga ttcaaaatcg ttttggccga ctcttttgga
    15781 ggagtgattt tatagataag ataagttgaa taacttgttg tatctttgac agtgttttta
    15841 ttcctaactg ctttaattgt aaatggtaca gcaatgagag caaataaagc gatgagagct
    15901 aaaatatttg cttttcgctt tttataaaga tttgcaaaca aatcagctac tgaataatgt
    15961 tcaaacatga ttttttctcc tttgtttagt agatactagt tttcctttgt aagcattttt
    16021 gctacaaata taatcacaag aacaattccc cagaattgca ttgtaaataa attgaagaaa
    16081 ctttctgaaa agctgcttct tggcataaag aatagattat tcaagatgag tagggataaa
    16141 gcaaatagga ttgtccttga gcgataggct acttgcagca tggctataaa taatacgccg
    16201 agtaagaaac taagcagaaa gactccaatc ataccatagt cggtatacaa ctccatgata
    16261 taactacttc cgataccatg ccctttcaag tattccttgt tcaagacaag ataggataga
    16321 ttgtgggcat aactattact atcaatagct agttccacac tattggttgt atgttcaaag
    16381 gcttttcctc cgaaaatggc tcccaaactc ccccttgcaa aataatcaag aacaggacca
    16441 aaagtaaaat tacggaaatc tcggtaaggg aggctactgt taaatagaaa acctcgagcc
    16501 agaacaccaa aactagtccc ttgtttatag ataaagtcaa gtaagatatc ccagaaacct
    16561 gtatgggaaa cttggacatt atcccgtaca taattgagta ctcccatcgc taacatgaga
    16621 ataggagaac ctacaaaaat cgctaacttt tctttaaacc caatccattt tcctttttca
    16681 gtttgctccc gcataaagta ataaacaaaa gcaaataaaa tacttaaaat aaagggattt
    16741 cgtgtcccaa ttgccaaatg aatagtatta gctgcaataa aggagacaag cactgctgtg
    16801 gcctgcaatt tctttggctt ggttgccaga tacatacaca ttgcatagac cgtaaaggta
    16861 gacaaaatgt aggtaaaata aggcagttta ctttcaaaat ttgcatagta ggcatagtag
    16921 gaagtctgca aacgatacaa gagccgttca aataaccgaa tgaaatagaa aggataagtt
    16981 agaagaaaaa ctcctagtga tacaaagcgt aaccgcttga tataaacctc ttttagagaa
    17041 tttcctatat ttgctacttt tattttcttc ctagctatga agtaacgagc caggacgcct
    17101 cctgttgtca agcccagaat cgaaaccatg acaactataa aggcaaaacg ataggctatt
    17161 ggatgatagg tgtccaaagc accatcccta aaataatcaa tggtcggtct tgataccaga
    17221 aatacaaaaa tggttaaata gaaaataaaa tggactaagt aatacttgat atcattccaa
    17281 caagcaatta agctactaac caacaagaac aataaagtag aaagtaagct aacattatta
    17341 ttattaaaca gatacacaat tccacttact agcgtcaagg cataactgac tatggtcaaa
    17401 ctaaataata atcgtttccc atcaatcact tggtcacccc cgttctaatg taatttttta
    17461 gatttttcaa tatttttcag taataagaat cggtataagg aaatatttat gaatagggcc
    17521 aaagcactaa ttcttctccc cttacggaaa attggattcc tagaaatagc aaaagcatag
    17581 ccttttaaaa aacgatgaat ctgagaatag gcttcaaact gtttatactg atcatctagc
    17641 aacatcttat ccagaataaa gaagtgagca taggccaatc tgaaaaaagc gacctctttc
    17701 aagtcaggat agtttttcac aacttcatta taaaactttt ggtagatatc aatataggct
    17761 aaatccttct ttgcataggg tttggtcgta atactatccc ctctatggaa atagtaataa
    17821 tagggtttag tattaaccac atacttcttg gccaacttga ttaaatcaaa atggtaatag
    17881 gcatcttcgt aaatcaaccc cttaggaaag gatagggcag ttgcaatttg tctcttgatt
    17941 agcttattgc aaatcgtccc aggtattttt tcacctatga ggtattcctt tagaaatgtt
    18001 tgagaatcac agacaaaata gtcatcctga ttggctgact gtgggctttc atcattagca
    18061 tagacattca tgacaccaca gcttgaaaca tccgcatctt cttgaactaa ttgctcatat
    18121 aagctctgaa tcatttctgg atggatataa tcatctgagt caataaaaat cagataatcc
    18181 ccgtgagcct gcttcatccc atcatttcgt gcttgcgaca atccttcgtt ctttttatga
    18241 agcactgaca ccctgtcatc ttgttcagcg attgaatcac acaagcgacc actttcatct
    18301 gttgcaccat catcaacaag aataatttcc agattttgat aggtctgctt ctgaatggaa
    18361 gctatcgatt tttctaggta ctgcgccaca ttatagactg gcacaatcac actaattaat
    18421 gcagtttcca tgctactcct ctaatagttt ttctacttgt tcgatttgtt ttgtaattgt
    18481 aaattgttga atgaattggc tagcctcatc gacatcaaag tttgaggtag aagtcatgta
    18541 attagtaatc gcctgagctg cctcttgatt gctctcaatg atttgtccaa atcgtccttc
    18601 ttgggataat tcctcagccc ctccaacgtc cgtagagata aaagggagtc ccagactcaa
    18661 ggcctccaca tacactccag gaaaaccttc ttgtttagac atagacaaaa gaactttcgt
    18721 ctgagataga tactgataag gatttttttg ataaccaagg aaatgtacat agtcctcaat
    18781 cccatactct ttgactcgtt ttttcagttc ctcttccata tcaccagctc cgataaaata
    18841 gagatgatag ttttttccct cttggtgtaa taatcgtatc acttccacta cacggtcaga
    18901 acccttattt tcctcaatcc gtccgatagt acagatactt tgaggagcaa tctcgatatc
    18961 gatcttctct tgagattttt ctagaatagt ctgaaaatca tatccattgt agattgtctg
    19021 taatttagaa gcataatctg gataaacttc cttgatagaa ttgctggtct tttttgaaat
    19081 ccctacaatt gtattcgcag catccaactg gcttctatgt gattctcttt tagagctatc
    19141 cttaagaagt tcttcaatac ttccatgaat ccaagatatc ttcttgactt ctcttctttt
    19201 agagaacaac agtggtggat tcataatggt aaaagaaact tcaacatcat aatcatcttt
    19261 tacaagcaaa cgacgagcca gtcttggaaa ataaattctc attctccaca aaaaagctcg
    19321 taaccatctg gtttggcgat aatcttgaag ggattttaaa atgcgtacat gctttggaac
    19381 agattcatat cccttgtcaa agtgctccat ttcaagaata tcaatatcat acttttctgg
    19441 atccagattt gaaacaatgg ttgatagaat cttctctgca ccacctccaa gagaaaaaga
    19501 ccacataaaa aataagattt ttttcttagc caccatattc tcccttgtat tctgtataag
    19561 acttatccat atcagcgatg acagcatcat gatgcggtac ctgcttgtct gctggtggag
    19621 gcgtcatata atccccaaaa gcagttctga gatagacatc atagccgatt ggaataggca
    19681 tctctgttcc ttcaaatggc aagaaaagat tgtcttcaaa agatgtgatt gggtacttgt
    19741 ttctcatgta gccaggacct gagcataatt ctgtaatgcc atcacaatca gccaaatcat
    19801 acttagtcat ttctttctca gcttttttcc agatgcgata acggagagat tttggagtca
    19861 aacccagtaa aatgcgactt ccccatttca tgagagcacc atgcttttct ggaatagttt
    19921 gcgcacaaaa gagtgaataa atcaaggccc aacgaacctg ttttttccgc tcagctggat
    19981 ctttcggata ataatccaaa ggcaaaacat ccaaggccag accatgtggc aaatccaaat
    20041 cctgctgata aggcttgata caggtggttt ccttgtcacg aatggtaata aaaagattac
    20101 gatcaacaaa atccttgtaa ctctttgaca agaaataacg ttcatctgca taacgaggcc
    20161 ataattctgc taatttctca taatctttac gaggcataaa aaagtctagg tcgtcgtccc
    20221 aaggaataaa tcccttgttt cgaagggtac caatagcgcc tccgccacag agataacaga
    20281 gcaaatcatg ttctttacaa aaggccacaa aatattcagc catctccaga ctacgagcct
    20341 gaattgcttt taaatcagtc atattgttca ttattctttc tatcgtatcg tttcattata
    20401 ccacaaacaa ggggtgaaaa tctattgcag actgtaaaaa atcaaagcct gactgctatc
    20461 caaatagcta tcaaactttg atttttctgt cttatactct tcgaaaatct cttcaaacca
    20521 cgtcagcttc accttgccgt aggtataggt aactgacttc gtcagtctta tctacaacct
    20581 caaaactgtg tttttagcag cctgcggcta gcttcctagt ttgcacttta attttcattg
    20641 agtattatct tatctcaagc ccatttgagc gagcttggtt tgatatttgt tttgatcaac
    20701 cagcaagccc aagcctccat aaacatcata ggcatctacc cagtcaccca gttctggaat
    20761 cgtcaatttt tcaataccat tttttgctcc atccaaaaca gataaaccgt ttgttaggag
    20821 gaaagtatag ggtacgttgg ttgaggtcat agcaaaaacc tttccaagag cttcagaacc
    20881 agtgaaaagt ttagtgggat ctttaatttg ctctaaaatt gctgttaaaa cttgttgctg
    20941 tctttttgta cggccgtaat ccgcctcatc atcatcacgg aaacgagcat aattgagcag
    21001 ggtcgagcca ttcatctgct gttttccgac tttaatggtt tgggttggag actcagtctc
    21061 ggtagcatat aaatcatctc cgactgtagc ttctgttagg ggacgcccat tcaatgttga
    21121 aaattgagca tcaatcgtca ccccatcagg gaaaagcgtg tcaatcgctg tggcaaaggc
    21181 ctggaaatca accaaggcgt agtacttaat gtccaagtca aaattatctt ttaagacttg
    21241 gcgaaccatt tctgcccctt tttgcccctc ttgttctcct aactcgtagg ctacgtttaa
    21301 cttgttatct gtctgttttc taccattaat cacttgacta taaccatcta tatagaccaa
    21361 attatcacgc ataaaactga ctagcttcat tttcttatct gagcccccga catttaatac
    21421 cataatagag tcagttcgtg tctcaacact gttctggccg attcgaccat cagtacccat
    21481 gattaaaata ttaactccat ctctagtgtc ctgaccatta aagacttcta cttgagctgc
    21541 ccgggcatca gcagttttct ttgcgctagc atcttggtaa ccacgcaaaa acatgaatac
    21601 catggccaaa gccacacaga ccaaaagtga aaaaatcacc ataaaaattc gtttaagacg
    21661 gagcttccgt cttttctttt ttggagggaa agagagtgct tgtgatttgg attgtgagcg
    21721 actccggttc gcatagcttg gtaagtcaac ctgctcttct ctttcttgtt ccaagctaga
    21781 gctactattt cccctagcaa gagttagctt ttcttgcaaa taggcaaact catttttttc
    21841 tctctcattg agatagtgaa tattttttag caaataatca taacgcaact gctcatgatg
    21901 acttaaggga ttttctttac tcatcttctc tcctttccat ggtctgatat tggataaata
    21961 ggataggcac ccagaatttt atactggatt ccaatcgctt ctaattcttt ttgggcaaag
    22021 tggaccaagt ccttatcggt ataatccaca tcgataatga aaaagtattc acccagtgct
    22081 gtcttgagtg gacgactttc aatttttgtc aagtcaattc ctcgccaagc aaaggtcgac
    22141 agggccttat aaagtgcacc tggaaggttg tcaggtaatg tcaaggccaa actcatcttt
    22201 tcagtttgtg cttgcaaggg aatactagga ttttcagctc ctagaaccca gaaacgtgtg
    22261 aaattggctt ccatttcctg aatatcctcg gcaatcagtt ccaatccata ttcttcagca
    22321 gaacttctag gtgcaactgc tgcaaagggc tggtctggat gttcggaaat aaaacgggcc
    22381 gcataagctg tactagctgt tacctcgatt tgagcctctg gatattgttc atcgatgaat
    22441 ttctttcctt gagccaaggc ctgtggatgt gaaaaaatct tttcaatctt agtatggcct
    22501 ggaaccacca tcaactgctg atgaataggc tgaacgattt ctgctactgc ttggatgtga
    22561 gcctgatgaa aaagatagtc caaggtttca tgaacactac cctcaataga attttcaact
    22621 ggcaccacag aatagttcac taatccttgc tcataagcct tgatgacatc tgtaatgttg
    22681 gcaaaagcct gcaattcctc atgaggaaaa gctgtctgca caacgtggtg tgaaaatgat
    22741 cccttgggac ctagataagc aattttcatc ttagttcctc tataatttcc tctgggctta
    22801 gcttggtcac atccaaaacc cgactagcca cttcctcata ccaagcctgt ctttcttgaa
    22861 aaatagctac tagttcttcc ttgctattat ttagaaaaag cggtcgctga ttgtccttat
    22921 cagctacgat acgtaggtag agggtttcaa aatctgcttt caggtagatg ttatctgtat
    22981 tagtcttgag taagtcacga tttctctgag aaataaccac tcctcctcca gttgacacga
    23041 cttggtctgt ttgtagtaaa tcagctagga cttctgattc tacctgacga aaggctgctt
    23101 ctcccttttc agcgaaaaaa ttcgcaatgg acatacctag gcgcttctca atcagagcat
    23161 ccatatcaag gtaattaggg tccaagcctc ttgcaatagt cgattttcca gcccccataa
    23221 accctaataa caccttagcc atgaatcaag ctctccaaat catcaaagaa actaggatag
    23281 ctggtattga tggcttctgc acggtcaagc tccacctctc catctgcaac caagagggct
    23341 gcgatagctg tcatcatgcc gatacggtgg tcaccaaacg tattgactct agcaccgtga
    23401 agagctgatt tccctttgat aatcatccca tctgccgtag gagtaatatc tgctcccata
    23461 ctatttaagg cgtctgccac aacctgaata cggtctgttt ccttgacctt gagctcctca
    23521 gcatccttga taactgttac accttgggct tgagtcgcaa gcagggcaat aatgggcaat
    23581 tcatcaatca atcgtggaat caaagcgcca ccaatctctg ttcctttcaa gtcagaagac
    23641 tcaacagtca aggtagaaga tttagcgact ggatcgattt cagttatttc caattttcca
    23701 cccatggcac gaatgacatc aataataccg gtgcgagttt cgttgatccc cacattctgc
    23761 agcactagac gagaatttgg aacaatcaaa cctgcgacta accaaaaggc tgcactggaa
    23821 atatctcctg gtacgaccac cttctgtcct gtcaattttt gtggcccttg gactgtgatt
    23881 ttcttactgt ccacacttaa atgaccacca aattgtttca acatatcttc agtatgatta
    23941 cgggtgcact ctttttcgat aataactgac tcccccttag cttgtaaggc tgcaaacatc
    24001 aaggctgact tgacttgggc agaggcaatt ggcaactcat aatgaatagg tcttaggttt
    24061 ttcgtccctt ttaagcgaag gggaggcaag tctcgttcag tttgccctga aatgctgacg
    24121 cccatttttt tcagtggaag ggtcacacgg tccataggac gtttggaaag actatcatct
    24181 ccaaacatct ctacttcgaa atctgcacca gcaaggacac ctgaaatcag gcgaatcgag
    24241 gtgccagaat ttcccatatt aagggcattt tgcggcgctt ttaagccagc catgcctaca
    24301 ccttgaatgg taataacccc atctttatcc tcaatttcaa caccaaggtc acgaaaaacc
    24361 tgcatggtcg aaagaacgtc ttcacctcgc agaatatcat aaaccttggt ctcaccctca
    24421 gccaaacttc caaagataat ggaacggtgg ctgatagact tgtcacctgg gacgcggata
    24481 ctaccatgta aatggcgaat atttgttttt agtttcatac tggacctcat acttgcaata
    24541 cttttaccta ttttatcata aaaagccaga aattccttaa aaattcctga ctttaggatc
    24601 gttcttttct tatttcagca attctgaaac tggttcaaaa acaatttttt caatgtcaga
    24661 aaggtaaatg gccaattgtt gttgcttggt aaagaattct gacaagaggc tatttccttg
    24721 aatctgttta ccaaagcctt ccatcttagc ttggaaggac gcatctggca tttgacctgt
    24781 ctgtgctagt ttttgaattt cctcttgaaa ggcaagatat tccgtaaaga ttttgcttgc
    24841 ctcagcatct gctgcaatcg catctttagc tgctttaaca gccttgtatt ctggtaatcc
    24901 gcgtagaccg cgactgagtt cgtttgcact atcgtaaata tttgacatgt tcttctcctt
    24961 atttgatgac gactgtatag tcggtatttt ctgttatgag atgctcagct ctttccaagt
    25021 cttgagcatt tttaaatgaa atttgtagga ttccgtgaat atcctcacga ttttcctcgt
    25081 tgatgtggat gttaaccaag gaagttccac gtagcagttc caaaatccgc aggatgacat
    25141 cttcttcatc aggaacgtca acatagaggt cgtaagagct atccacacca ccacgcttat
    25201 ggatttccat ggtctggcgt tgttcacgcg cttggttaaa aaagttccaa atttgctctt
    25261 catctccctt actaatggcc tgaccaatca cttccaaacg ttccttgaaa tcctcaattc
    25321 tatccagaat ggtctcgcta ttggacaaga gaatggaggt ccacattcct ggctcgcttt
    25381 ccgcaattcg ggtcatatct cgaaaaccac ctgccgcaaa gcgccttgcc atctcatgct
    25441 cttgagcata gaccgcagtc tgctccatga gactagaagc caaaatatga ggaaaatggc
    25501 taatctgaga agtgacacga tcatgctcct tggcatcaat ctcgataaaa cgagcatgaa
    25561 gacctgaaag cagatccttc atttccttaa gcgtgtcctg acttgtcagg cttgaaggtg
    25621 taaagatata ataggcattt tcaaaaagat tgacatctgc cgaagcagcc cctgtcttgt
    25681 gactaccagc catgggatgg gccccgacaa agcgaacaga cttgccagcc aaatactgct
    25741 ccgccgcatc cacaatggtt gacttggtcg aaccagcatc tgaaataata acgccttctc
    25801 gcaaatccaa attggccaac tccttaatga aagcaatagt ttgtttgatt ggcaagctga
    25861 ggataatgac atctgccaaa ggagcaaaac tagcaaaatc atccgttgca cggtcaatca
    25921 tgccttcttt caaggcgata tctctcgaag cttgactacg attataacct aaaatttcat
    25981 aatctggatg atcgcgtttg ataccaagtg ccatagaggc tccaatcaac ccaagacctg
    26041 cgatatagat tgtttttgcc ataggaactc cttaatagtt ctttgtatag tctcggtgtt
    26101 tggctaccgc ttcttttagt tcctcaagat tatctgatga gaatttttcg aggatttctt
    26161 gcgccagaac cgttgctaca actgcttcca tgaccattcc tgcagctgga agagcagtcg
    26221 gatcacttct ctccacggtt gccttgtaag gttcatgggt ttcgatatcc acactcataa
    26281 gaggtttata aagagtagga atgggtttca tgaccccacg aacaacgatg ggttgcccat
    26341 tagtcatacc accttcaaaa ccacctagat tattggtacg gcgagtataa ccgtcttctt
    26401 tagaccagag aatttcatcc ataacttggc tgcctttacg ataaccagcc tcaaagccaa
    26461 gaccaaattc caccccttta aaggcattga tagagacaac agcttgagcc aatcttgcat
    26521 ccaattttct atcccattgg acataggaac caagaccaac tggaacgcct ccgacgactg
    26581 tctccacaac cccaccgatg gtatcaccat cacgtttgat ttggtcaata tagtccttga
    26641 tttcctgttc tcgttcttgg ttgacaatag aaacttcaga ctgggcagct ctttgcttaa
    26701 tttcagcgac tgtcagattt tcaggaacat cgatttcctt gccaccaaag accacgacat
    26761 ggttggcaat ctccatatcc agctcagcca agaggcgttt ggctactgca ccaactgcca
    26821 cccgcatggt ggtttcacga gctgatgaac gctccaaaga atttcgcaaa tcatcaaaac
    26881 ggtacttaat ccccccaacc aaatcggcat gacctgggcg aggatgagta attttccgct
    26941 tgcttttaag acggtcttca atgtcctccg cagacatgat gtccagccat ttctggtggt
    27001 ccttattgat gacatccata gtaataggcg cccctgtcgt cttcccgtgg cgaacgcccg
    27061 aagtaaagac aacctggtca ttctcaatct tcatacgacc accacgaccg tagccaccct
    27121 gacggcgtct aaggtcctca ttgatatcct cagctgtcaa tggaagtcca gctggaattc
    27181 cctcaataat agctgttaga cgggggccgt gtgattctcc tgcagttaaa tatctcatac
    27241 actctcctta ttttaccaag tagtctttca tctcttccag agaaactgga tgaatggtcg
    27301 ctgaaccaag ctctggcacc aagaccaatt tcaaggtgtt accacgcgct ttcttgtcat
    27361 gagtaagagc ctgataaagc ttgtcaactt cccaattttc atagtcaaca ggcaagccga
    27421 atttctgaca catctctgtg atagattggg taatgctagc tggcatgagg cctttttcct
    27481 cagcaatctt ggaaatctgt accattccca tggcaacagc ctctccatgc atgaccttgc
    27541 cataaccggc agtcgcttcg atggcatggc caatagtgtg gccaaaattg aggtaaagac
    27601 gaataccatt gtccaactca tcttcaacca ccatcttgcg cttcacctga caagaatgtt
    27661 caatcaaggt ctctgcatgt tccaaaatgc tctcaacaga accattcagc cccgtcaaga
    27721 gagcccacag ttctggatcc tcaatcaagc catacttgat aacttcaccc atcccttcaa
    27781 tcaactctct ttttccgagg gtttcaagaa caagtggatc aatcagaacc ccatctggtt
    27841 gagcaaaggt ccccaccata tttttagcaa atggtgtgtt aacgcctgtc tttccaccga
    27901 tagaagaatc aacctgagct gtcaaactag tcggaatctg aacaaagtga ataccccgca
    27961 tataggtaga ggctacaaag ccagccaggt ccccaacaac gccaccacca agagccacga
    28021 ttccatcgct acgagtcaga ccttgcttga ctagaaattc atagactttc tgaacagtag
    28081 ttaaattctt tctttcttca ccttctaaga aatcaaaaac agctacctga aaaccagcat
    28141 cttctaggct gagcttgacc ttctctgcat agagagaggc tacatggtta tctgtcacaa
    28201 tgactacctt ttgcggttgc cagagttctc gcaaccactg accagcctgg gccatacaac
    28261 ctttttcaat ctgaatatca taaggatgat gaggaatatc gattctgatt ttcataggag
    28321 agtctccctt tctttattgg tatttttctg ttaaagactg ccaaatctct tctgtcggca
    28381 tttccttgcc tgtccacagt tgaaaagctt ctgcagcttg atagagcaac attcccagac
    28441 cattgactgc tggattgccc tgacttctag cccatttcaa aaacggtgtt tcaaagggtt
    28501 ggtatatgat atctgcaact aagagagttt ctggtaagac tatgttttca ggaacaggag
    28561 aggattggcc atccatgccc acactggtgg cattaactag caaatccgac tcggcaatcc
    28621 ttgcttgcag ttcagaaaca tactctaaag cacacaaatc cactttaaaa cctgtctgct
    28681 cctgtaactt gtctaggtaa ggtcttgttt tttccataga aacggaacga acaaagaccg
    28741 aaatctgact gacgccatcc aaaatagcct gtgccaagat tgatttagcc gcaccacctg
    28801 cacccagcag ggtcatcttt ttacctgaaa ttgtaaaaga aggcaagcac ttaaaaaatc
    28861 ccttgccatc tgtattatat ccaattaaat tgccattctc attgacaacc gtattaaccg
    28921 caccaatcaa gcgcgcttca tcgctcagct tatccaaata aggaatcacc tgctccttat
    28981 agggcatgga cagattgatg ccaaacatct ggtagcgacg aatattggcc actgtttctg
    29041 ccaagtcact cgcttcaatc tcccaagcca cataagcacc gttggtagct gtcgcctcaa
    29101 aggctctatt gtggatgaag ggagaaatag aatgcttaat aggattggca acaactgcag
    29161 ctaaacgtgt atagccatca agcttcatcc aaaatctccc tgattttttt catgctagct
    29221 agagaaatct gcccaggggc actaacctca tccagactgg caaaagacca actcgaacca
    29281 gtcacatccg cagtgatacg agagaccttg cccaccttac ccatagaaat ggtcacatat
    29341 tcctgttcag gattgagggt tttaaagcct cgtgtatagt tcatcaagtc taagacatcc
    29401 tgctccgtgt gagccatcac cgcaacctta acaagttttg gatttaggat cgtcaactct
    29461 gacaagattt ccatcatgtt ctcaggtgtt tcttggaaat tatggtaact caaaacaaga
    29521 tttgggaagt ccagcatttc ctcaaaaaca tccttgtagc tatagtactc aaaatcaata
    29581 tagtctggtt gatagaattg cgcaacttcc ttgattagat ggatatactc ttctggagaa
    29641 aggtcgattt ctccaccttc ggagcgagtt cgtagcgtga aaaccaactc acggcctgcg
    29701 aatttttcaa aaatggctgg agctacctgc aaaatcgctt ctttaggcag atagtcggca
    29761 cgccattcaa tgatgtcggc atccaggtac ctcgtggcat ccagagcctg agcctcctct
    29821 aaacttcttg gcattactga aacgattaat ttcatttact aaccttcata ctaatcacct
    29881 tgaggtaatt actactttca tcttttttat tataggcaaa atctgctgga agaccatatt
    29941 tgtttaaaat ctggtaactt cttcctgcaa agcctttatc aatttgttct gtaaatttct
    30001 gacgggaaac attggcagca ttggtactgg caatgataat ccctcccgga tttaaaatct
    30061 caagactctg ggaaatcaac ttgtgataat ccttggccac agagaaagtt tgttttttat
    30121 tccgagcaaa gctaggcgga tctaggacaa tcacatcgta ggtcaagtct ttgcgtttgg
    30181 catatttgaa atactcaaag acatccatga ctataaaacg atgctcgtct gtgctgagcc
    30241 catttgcctg aaaatgcgct tgagacaatt ctcgtgaacg tttggctaga tcaacagaag
    30301 ttgtctggct agctcctccc atggccgcag ctactgaaaa agccgctgtg taggaaaaca
    30361 tattgagcaa ggatttaccc atagccaagc cgtcaactaa actaccgcga acctcatgct
    30421 ggtctaggaa aattcctgtc atcaagccat cattcataaa gacttgatac aggacaccat
    30481 tttctaaaac agtgaaaaag tcaggtgctt cttgaccata aacatgggca gattcatagt
    30541 caaaaccctt aaagcggatt ttctcataag cccctaaaac ctcagggaaa acctgtctaa
    30601 aggcttctga tatagtctga cgaatctgat aaacataaga gttataccaa gaaaagacgg
    30661 cgtagtcgcc ataaaggtcc actgtcagac ccccaaagcc atctccctct tgattaaaga
    30721 gacgaaaggc agttgtcaaa tcatcttgat agtaagcgtt tctcttttct ttggcttttc
    30781 taaacaacgt ttcaaagaaa gtttgattga aggccacctt gtctttgctg acaaaccagc
    30841 ccaagccctt gttttgctga gaaaggtagg cagtcccaag aaagtttcct tcctgaccct
    30901 gcacctctac ttcctgatcc ttaagattga cattctcaag atcactggat tctagtaaaa
    30961 ctagcccctt agcaagcttc ttttcaaccc ttttgctgac tcttattcta tccataacta
    31021 ccattatatc aaacttttag acaattctca aaaaagaaac tacccttgct tttttactct
    31081 tcttttaaaa aatggtatac tagacttcct gcaaaactag gaagtaaatg tgtaagaatc
    31141 acaataaaaa atgctcttcc atcttggagg agcattcttt ttatcaacga aaatcaaata
    31201 gcaaactatg aaactagcct caggttaacc gtgagattat aggtagagag gttgtatcag
    31261 caatatgtgt ctgtcaaatt tagtgacaaa ggtagtagaa gaaagatgaa gaaataaatc
    31321 tgcttcaata ggtatctgga aaatttgatt ttatagagaa gccttttgtt acaaactcaa
    31381 tatactatca ataaataata ttatagaagc aacaataatt ataatttcac ctatctgcat
    31441 cattctattt cgaactctaa atatatgttc tatcaaaaat acttggatca cacacattat
    31501 aggaattaac gtttttgaaa ttgaaaaata tccaaataaa taaactataa acaacaataa
    31561 tagaactata ttatatttct tattcaaaac attcctccct atatttttga ttaccaatct
    31621 taatcattta caactacatt ctaacaaact ataaaagcgt ttgtcgaatt gaatttatca
    31681 agcaagcaac caactatctc atcttttttc tatttctgcc aatatgcgtg acaggtaata
    31741 atgatagcca aaaatagcaa gagcaagcaa gacgataaga gctcctactc ccaagttgat
    31801 ggcaaggata ggggagagag actgaaccaa gaatatgctc ccaattacaa gggccatcag
    31861 gattgcacta taaataaaca ataaaactat ggcgactatg ccatttgaac gattcaccag
    31921 gtccgtaatg ctactccaat tggttgacag atttttaacg tccttaaagt aatggtggca
    31981 agaaaagatg acactggcaa tgatccagac tacaagaagg taaatcatcg aaaggatgga
    32041 caagcctaga tatagagaaa gaccgagcaa agtcagaact ggtaaaaagg actgaacagc
    32101 aaagataatc caaaatttca ctttcacata acgagcaaag tcaaagggta aactcttcag
    32161 aaaatcaaca tttttcctct ccaaggacaa ggcaattgaa tgcaggctgg tgatattgtt
    32221 attgacaact gctataaaca ctcctacaaa aaacaagggt aaccagtatg gaggatgaat
    32281 gtctggaact atctgagaat ctcggatttt ggaaatcaga ctgatcatca tgagataagg
    32341 aaggaaagca cttgtaaaaa gcactgtaat cacgccagtc ccctgtccca agagggtgag
    32401 gtggtagcgt aaaaccatac ggaaaaagcc ctttttagta gttgaaatcc tctccttgct
    32461 gcgacgttct tttttgacct tctcctcact gttaagcagg atcacgtcat aaaaacgagg
    32521 aaggaccttc tttttggtca ggtaaagcaa gaaaacagtt aaagctatcc aagcgagcag
    32581 acccaatatg gcttctattg aaaaaggctc cactgctatt ttgtaaaaga tatgaagagg
    32641 ataaaggaga aatggaatgt ctctaacttt gtcaacaata cttccaaaag tcgactgtag
    32701 aaagaagaca aatattaaag gtatgagaac tcctatccca atcatcacat tcgaaaaaat
    32761 agactgatac tttctgaagg ccgtagtttg agccaagaaa tgcactgcca ctaccatcac
    32821 tagagccaca gagacaaata ataaggtcaa ggacagtagc atcaaaggca aacccagcca
    32881 aagagaagga gctagactaa tatagagggc tagaaaataa gctaggattg gtacaattcc
    32941 agttagagct ggaaaaagga cagacagtcc tttagcaatt ataatctctg attctttaaa
    33001 ggcatagggc ctataggagg ccaggtcctt actctcataa aagacattgt aaaatgcagt
    33061 taaagaagtt gaaaaggcaa tcactagtaa aatagcaacc acgaaactaa aataaacagg
    33121 catttcctca aaaggaaaat gaatggctat agtactaaaa aagatgagca tcaagagact
    33181 ggaaaaaatg taagaactta ggactctagc ggaaacattt actttttttc cagtattttt
    33241 agcttgcttc tttcgtaggt tagccagatt agcttcttaa gatgaataga ggatattgat
    33301 atcaactaat tttttaatga ccttgagacg catctgaaac ctcctctttt ctaccagcaa
    33361 ggctaaggta gatactttcc aaagactggt ctgggttgtc ttttctcaag tcctctacct
    33421 taccacaata aatcaaatgc ccctttttca aaatagcaat ccgatcacag acttgctcag
    33481 ctacctctag cacatgagtt gaaaacaaaa ctgtcttacc tttttgcgca tgctccttca
    33541 tcatttgctt caaatcaaag gcagcctggg gatccaaacc agtcaagggt tcgtccaaga
    33601 cccaaatatc aggatcagac aagagtgccc cgatgacaaa aactttctga cgcattccat
    33661 gagaaagggt ttcaataacc tgataacgat tttcagcaaa atcaaaaacg ttcaataacc
    33721 tagctaaact agcttccaag tcagagctgc tcaaatcata ggatgaagca atcaattccc
    33781 aaaattcatt ggccgttaaa cgtaaaaata agtcaggcga gtctgctacg tagccaatct
    33841 ttcgtttgat cgccaagcga ttttccgata aatcctgacc atctaccaaa atacgaccac
    33901 tgctgggtga aatgatactg actagggatt ttatggtggt cgattttcca gccccattat
    33961 gaccaatcaa gcccataatc tctccatttt caatctgcaa attgagatta ctcaaggcct
    34021 ctttatcacc atacaactta ctaacatttt gaaattcaat catgggacaa ctcctatctt
    34081 tatctgtatt atatatcgct atactagcac tttgatacaa aaaaatcaat actttatttt
    34141 aagtagttaa aatagtttcc ttctatctta cgcaaacgtt tgcgctaata gatactttat
    34201 gatagaataa agaacaagat tgacaagtaa gaggaaacat tatgcaaaat caaacactca
    34261 tgcaatactt tgaatggtat ctgccccacg acggtcaaca ctggacgcgt ctggctgaaa
    34321 atgctccaca cctagctcat ctggggatca gtcacgtctg gatgccacca gccttcaagg
    34381 caaccaacga aaaagatgtc ggctatgggg tctatgactt atttgactta ggagagttca
    34441 accaaaaagg gactgtccgc accaagtatg gtttcaaaga agactatctt caagccattc
    34501 aagcccttaa agcgcaggga attcaaccta tggccgatgt ggttctcaac cacaaggctg
    34561 ctgctgatca cagggaagcc tttcaagtta ttgaagtgga tcctgtagac cgtacagttg
    34621 aacttggaga acccttcacc atcaatggct ggactagttt taccttcgat ggtcgccaag
    34681 atacctacaa tgacttccac tggcactggt accacttcac aggtacagac tatgatgcca
    34741 aacgccgtaa gtctgggatt tatctcatcc aaggggacaa caagggctgg gctaacgagg
    34801 aattggtcga taacgaaaac ggaaactacg actacctcat gtatgccgac ctagacttta
    34861 aacatcctga agtcatccaa aacatctatg actgggctga ttggttcatg gaaacgactg
    34921 gtgtagctgg tttccgtttg gatgccgtta agcatattga ctctttcttt atgcgcaact
    34981 tcatccgcga tatgaaggaa aaatacggtg acgatttcta tgtttttggt gaattttgga
    35041 acccagacaa ggaagccaat ctggactatc tcgaaaaaac ggaagaacac tttgaccttg
    35101 tcgatgttcg tctccatcag aatctctttg aagccagtca agctggcgca aactatgacc
    35161 ttcgtggcat tttcacagat agcctggttg aactcaagcc tgacaaggct gtgacttttg
    35221 tcgacaacca cgatacccaa cgaggacaag cccttgagtc taccgttgaa gaatggttca
    35281 agccagcagc ctatgccctc attttgttac gccaagacgg ccttccatgt gtcttttacg
    35341 gagactacta tgggatttca ggccagtatg ctcaagaaga tttcaaagaa atccttgacc
    35401 gcctcctagc catccgaaaa gatttggcct atggagaaca aaatgactac tttgaccatg
    35461 ctaactgtat cggttgggta cgttcaggtg ctgaaaatca atccccaatc gcagtcctta
    35521 tctcaaatga ccaagaaaac agcaagtcaa tgtttgtcgg tcaagaatgg actaatcaaa
    35581 cctttgtaga tttacttggt aaccaccaag gtcaagttac aattgatgag gaaggttatg
    35641 gacaattccc tgtctcagct agatccgtaa gtgtctgggc agtcaatacc atctaatagc
    35701 tcataataac caagctaggt ccaagcggat ttggcttttt tgtattcaca aaaagaccta
    35761 cccaaatgga tagatcttta cttgattaca atttacctgc tactgcatcc aacaattctt
    35821 ggatcttagg ttggttgctt cctcctgcca tggccatgtc tggtttacca ccaccacgtc
    35881 catcgatgat tggtgctaat tctttgacaa ggtttcctgc atgaaggtct tttgtcttgc
    35941 ttgctacaag gacattgact ttgtcaccga tagcggcaac taggacaaga agatcagagt
    36001 agtctttttg tttccagtta tctgcaaagg tacgaagggc acctgcatcg gatacagaca
    36061 cttgactagc aatgtaacga tgaccgttga cttccttaac atctttgaag atatcgcctg
    36121 cggctgcagc tgcggctttt tctttcaact cagcattttc tttttgaagt tgacgaagtt
    36181 gttcttgaag tccttctacc ttgtgaggta cttccttgac ttgaggtgct ttcaaggttg
    36241 ctgcgacagc tttaagagca tcctcttgtt cacgataggc ttcaaaggct tccttaccag
    36301 tcactgccaa gatacggcga gttcctgaac cgattccttc ttctttgaca attttgaaga
    36361 gaccaatctc agaagtgttg ccaacatgag taccaccaca aagttcaata gagtagtcac
    36421 caatagtcac gacacgaact tccttgccgt atttctcacc aaagagggcc atcgctccca
    36481 tttctttagc agtgtcaata tccgtttcaa ctgtcttaac ttcaagagct tcccagattt
    36541 tttcgttgac ttgctgttca atcgcacgca attcttcagc agttacagct tggaagtggg
    36601 taaagtcaaa gcgaaggaat tcaacttcgt taagagatcc tgcctgtgtt gcgtggtttc
    36661 caaggatatt gtgaagggca gcgtgaagca aatgagtcgc agtgtggttt ttcatgacac
    36721 ggtgacggcg attgctatca attgccaagg tatattcttg gttcaaggca agcggtgcaa
    36781 ggacttcaac tgtatgaagg gcttgaccat ttggggcttt ctgaacattg gtcacagtag
    36841 ccacaacctt acctgactca tccaagattt gtccgtggtc agcgacctgt ccacccattt
    36901 cagcatagaa tggcgtttcc gcaaagataa gtgaggtagt tccttctgat acagcttcta
    36961 cttctgcatt gtcagcaacg atagccacca atttagaaga caattggcta gcattgtggt
    37021 tgaagacact ttctacagtg atgttttgaa gagtttcatt ttgcataccc attgagccac
    37081 ccttgacagc tgacgcacgc gcgcgttctt gttgttcttt catggctgct tcaaaacctt
    37141 cacggtctac agtcatacca gcctcttcag caatttcttc agtcaattca actgggaatc
    37201 cgtatgtatc atagagttta aagacatctt gaccagcaat gacagattga cctttttctt
    37261 tcaagtctgc cacaatgcct tgggcaaagt gttgacctga gtgaagggta cgggcaaatg
    37321 attcttcttc gctcttaacg attttctcaa taaagtcacg tttctcaagc acttctgggt
    37381 agtagctttc catgattttt ccaacagttg gaacgagttt gtaaaggaaa ggctcgttga
    37441 tacccaattt ttgaccatgc atagaagcac gacggagaag acgacgaaga acataaccac
    37501 gaccttcatt tcctggaagg gcaccatcac cgatagcaaa tgaaagagaa cgaatgtggt
    37561 ctgcgataac cttgaagctc atgttgtcgc catcttggtc ataaacctta ccagacaatt
    37621 tctcgacttc acggataatc ggcataaaga ggtccgtttc aaagttggtc ttagcccctt
    37681 ggataacagc caccaaacgc tccaaaccag cgcccgtatc aatgttctta tgtggcaatt
    37741 ccttgtattc gctacgagga acagcagggt ctgcgttaaa ttgtgacaaa acgatgttcc
    37801 agatttcaat ataacggtcg ttttcaatat cttctgcaag caggcgaaga ccgatatttt
    37861 ctgggtcaaa ggcttcccca cggtcaaaga agatttctgt atctggtcca gaaggtcccg
    37921 caccgatttc ccagaagttg tcctcaattg gaatcaagtg acttggatcc actcccactt
    37981 caatccagcg gttgtaagaa tctttatcgt ctggatagta ggtcatgtaa agtttttcag
    38041 cagggaaatc aaaccattca gggcttgtca aaagctcata agcccaagtg atagcttcgt
    38101 cacggaagta atccccgata gagaagttcc ccaacatttc aaacatggta tggtgacgcg
    38161 cagtcttccc tacgttttcg atgtcgttgg tacggatagc cttttgggca ttggtaatac
    38221 gtggattctc agggataatg gtcccgtcaa agtatttctt aagggttgct accccagagt
    38281 tgatccacaa aagagttggg tcgtttacag gaaccaaact tactgatggt tctactgagt
    38341 gacctttggt cgcccagaaa tcaagccaca tttggcgtac ttgtgcacta gatagttgtt
    38401 tcatattgtc tccttattta cttgtttaat gtgattggct ttccagcatt tccacatagt
    38461 caatcgcgac acagagggaa atgactaggt ctgcataagc gtcctcaaga accgttacgg
    38521 tataggtaga ggtcagatgg aagagttcct tcttaatttc cgcaatcagc tgatcgcgat
    38581 catccagcaa tttgaaattc aaatcccaga tattgccctc gatacgaaga cctagattat
    38641 caaactcata cttatctcgc cagaaggtca acttcttacg aatgacaaaa ctcgagccat
    38701 ccctaagctg aatctcaaaa cgaggaagca aggtcaagat ttctttacta atctcactga
    38761 cttgttcacc agccgcatca tagatggtaa aggttttagg aatcttaaaa aatgatccct
    38821 ccacctgata ggcaatttct cccctgtcat ccttgatagc gaagcgttcg cctccaagac
    38881 gaaacttttg tttgacaaga aatgttttca tcaacacctc caaaaatcaa aagacaagct
    38941 catatcacga agggcgaaaa accgcggtac caccttcatt caatgaactt gtcattctct
    39001 tgttcttatg caattgtatg attgagtagc atgacttcct agcttagatg gctcgcagca
    39061 ccgccatttc tctggactaa gacaagtgaa aatcaattct caactttctt attataacgt
    39121 ttttttaagc ttgcgtcaac tggaaatgat ctccgttgaa ttagaccaat tccctacatc
    39181 tctgattact ttttcaggat atattttttc ttactgccgt ttttcttttt atcccaaatt
    39241 ttcatattac taaacacagc tactagaata tttccaaata taaaggtgcc tatcacccaa
    39301 tatatggact cagttgttag gtattgtcga tccaagccat cctttaaatg gaatagtata
    39361 acagtttggt taacaatcat aaaggttggc cagaaacttt ttttgaaaaa agtagacatt
    39421 ttcattattt gttgccgctt tctgtaaggt taatactcaa taaaaatcaa aaagcaaact
    39481 aggaagctag cctcaagctg tacttgagta cggcaaggca acgctgacgt ggtttgaaga
    39541 gtataggctt agtatactac taggcaagca aataaacaaa taaacaacta gaatagaaaa
    39601 agatagggct ctaaaaactg acttctattc cttaaaaacg aaccagcttg actgattcgt
    39661 cttcttacgt ttatctccta cttccgatac attttaaact gtaggaagag gtcgctatat
    39721 ttccctgtcc atttatggtc aaatttctca taaacttcta ggtgtttcat ggtttcaaca
    39781 tcgggataga aggccttatc ttcctttgtt tcctctggga gcaattcctt cgctggtagg
    39841 tttggtgttg aatagccgac atactccgca ttttggagag cattttcagg tttcaacata
    39901 aagttgataa aggcataggc tgagttttgg tttttaactg ttttgggaat gaccatattg
    39961 tcaaaccaaa gattgctggc ctctgtcggt accacataac gtagattttc atttttttct
    40021 aacatttggc tggcttcacc agagaaggtc acgccgattg caacattatt ctgaatcata
    40081 tagcccttca tctcgtccgc aacgatagcc ttgatatttg gagtcagttt gtagagctta
    40141 tccactgtct cttccaactg ctgcagatcc ttggagttga ggctgtagcc gagggaattg
    40201 agccccagac ccagcacctc acgcgcccca tcaaagagca tgatagaatt cttatactcc
    40261 ggcttccaaa ggtcatccca atgctcaggc gcttcatcta ccatggtttc gttgtagaca
    40321 attcctaagg ttccccagaa gtaagggatg gagaatttat tacctgggtc aaaggactgg
    40381 ttgagaaact ctggtccgat attttcgatt ccttcaattt ttgaataatc aagcggaacc
    40441 aagaggtctt cgtccttcat cttgttaatc atgtattcac ttggaatggc aatatcgtag
    40501 gtcgttccac cctgctttat cttagtgtac atggcttcgt tggagtcaaa agtctcgtac
    40561 tgaacttgaa ttcctgtttc ttctgtaaac tgagtcaaga gttcaggatc gatatagtct
    40621 ccccagttat agataaccaa tttttgacta tctcgactat tgattttact atctaaatga
    40681 gtcgcaattc cccacaagac aaggataatc gctgcaattc ctgctaaaaa tgaatagatt
    40741 tttttcatgc ttgctcctcc ttctcacgag agataaagta ataacctaca actaggataa
    40801 tactaaagag aaagactaga gcagacaggg cattgatttc taaggaaatc cctttccgtg
    40861 cacgagagta aatctcgact gacagggttg aaaagccatt tcctgttaca aagaaggtca
    40921 cggcaaaatc atccagcgaa taggtgaagg ccatgaagta acctgcaatg atagacggag
    40981 tcaggtaagg aagcatgatt tccttaaaca tctgaaattg actagctccc aagtcatagg
    41041 ccgcatgaat catgtcgcca ttcatttcct tgagtcgagg taagaccatc aagaccacga
    41101 taggaatgga gaaggccacg tgactagata gaacggtcaa aaagccaagt gaaaacttga
    41161 gttgggtaaa gagaatcaag aagctagcac caatcataac gtcaggcgca accatgagga
    41221 tattattgag tgatagaaag gcttcttggt atttcttacg agactggtag atgtaaatgg
    41281 caccaaaagt cccgataatg gtcgctatca aggctgatag gaaggccaag aaaaaggtct
    41341 gagccaaaat cagcatgagt ctcccatctc caaacatggt ttcaaagtga gtccagctaa
    41401 aacctgtaaa gctattcata tcatcaccag cattaaaggc atagccaatc aagtaaaaga
    41461 taggcaggta gaggaccaga aagaccagtc ccagataaag gttggcaaat tttttcatcg
    41521 ttctctcctt tccttagtca cccacatggt gatgaacatg gtcaggatga gaatcacacc
    41581 gatggttgaa cccataccat agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc
    41641 caaggtgata acgcggttcc caccaatcaa acgggtcagc atgaagagac tcaaacttgg
    41701 gataaagacc gactgaaccc cacttcgcac accgttcata gatagaggga agatgacatg
    41761 acggaaggtc tcccacttag ttgcaccaag gtcatagctg gcattgatga gattattatc
    41821 catatcgtct aagacattga aaatcggcaa aatcataaag gggagctcga tgtagcttgc
    41881 gacaaagata aaggaaaaat cggtaaaaag caactgttgt gaaccaattc cgataaattc
    41941 caagaattgg ttaatagagc cattttgacc aaaaatcccg ataaaagcat aggctttaag
    42001 gagcaaattg atccaggtag gaaggataat cagcatgagc cagagttgac ggtgcttgag
    42061 acgggtcaaa aagagggctg ttggatagct gataagcaag gtcacaaagg tcacaatccc
    42121 tgcataaagc actgagttga aactcatttt aagataggtc aagttttgtg acgcaaagta
    42181 agatttgtaa ttttctaaac tgaactggcc ttcgatgttg aaaaaggatt gaccgaaaat
    42241 caagaccaag ggtgccaata caaagagcgc aatccaaagc atgtagggta ctacaaagag
    42301 tttagagctt gttttcttca tctctttcct cctcgattgc attgatcaaa cctgcttctt
    42361 gctcttcgat ttctacgtac tcctcaatac gagcatcgaa ctcttcttcg gtttcattga
    42421 gacgcatgat gtggatgtct tctggttcaa agtccagacc gatttcctca cccacgatgg
    42481 ccttacgggt tgagtggatc atccattcat ttccaagttc gtcataggcg ataatttcat
    42541 aatgcacccc acggaagagc tgggtatcga ccttaacttg aagcttgcct tcttcaggaa
    42601 gggtaatgcg caagtcctct ggacgaataa cgacctcaac aggttcattt ggcttcatcc
    42661 caccatcaac cgcttcaaag cgtttgccgt taaattcgac caagtagtcc tcaatcatgg
    42721 tacctggcaa gatgtttgac tccccgataa aggtggcaac aaagtggttg attggctcat
    42781 cgtagatgtc cacaggggtt ccagactgga caatctcgcc atcattcata acgaaaatcc
    42841 agtcactcat ggcaagagct tcttcctgat cgtgagtgac aaagacaaag gtaatgccca
    42901 atcgttgttg cagttcacgc agttcgtact gcatgtctgt tcttaatttc aagtccagcg
    42961 ctgacaaggg ctcatccaac aaaaccacac ggggttggtt gatgatagca cgggcgatag
    43021 ccacacgctg acgttgtcct ccagaaagtt tgcggatgga acgtttttca taaccttcca
    43081 actgaaccat cttgagaact tccgctacac gttgctcgat ttctttctta tcaattttac
    43141 gcaagcgaag tggaaaggca acattttcaa acacattcat atgtgggaac aaggcatagg
    43201 attggaagac agtatgtacg tcgcgcttgt tggttggaat atcattgata cgaacaccgt
    43261 ctagcatgat atctcctgtc gtcgcatcca gtaaacctgc aataatgttt aggatagttg
    43321 atttccccga accagatgca cctagaaggg tgtagaattt cccttcttcc aactcaaagt
    43381 tgatgtcttt gagaaccttg gtgttgctgt cttcaaaaac tttagagacg tttttgaatt
    43441 cgataattgg ttttttcaat tggcataaat tccttctttt tcatagatta accgatcggg
    43501 gctctgtcag gtccccacta cctcttgcag ggagtaaaac cacctgcata catcttcgct
    43561 accgataggc tttcaccccc ctttccatga catagcagca gcttttcaaa tacagcttcc
    43621 tacttgcttt cacccaagat ccggacttct ctttcaagcg taatacctga gtgttccttg
    43681 actttttcga taaccgattg gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca
    43741 ttgatcataa atcctgcatg cttttctgac acttctacgc caccgatacg atagcctttc
    43801 aagccagctt ctgaaattaa ctgacctgca aaatgcccga ctggacgctt aaagaccgag
    43861 ccacaagatg ggtattccaa aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt
    43921 tcctgcttga taacctgatg ggttcctggg gctagggcaa atttaactga caagacaact
    43981 gcaccagact cctgaatagc tgaatggcgg taaccaaaag ccaagtcttt agcagacagg
    44041 gtttcgattt ctccatcctt ggtcaagacc ttacaagact gcaatatgtg agcaatctcg
    44101 ccaccatagg cacccgcatt cataaagaca gcaccgccaa cgcttcctgg aataccacaa
    44161 gcaaactcaa agccagtcaa actatgacgg agggcaatgc gagttgtttc aatcaagtta
    44221 gccccagctt ctgcttcaat ggtatagcca tcaacagaaa cgttattgag cttgtcacac
    44281 aagatgacaa atccacgaat cccaccatca cgaacgatga tattgcttgc attgccaaga
    44341 accatccaag ggatattttc ttggttggca aatttcacaa cgcgagccaa ctcaaaacga
    44401 tttcgtggaa agaccaaata atcagcctct ccacctactt ttgtataact atagctatgc
    44461 aagggttcct gaaaacggat atcaattcct tctaagattt caagcatttt ttctcttaca
    44521 gacatgtcac tcttcctttt acaaaattca ttccattata ccatttttag agacatttga
    44581 cgaccataaa aataccttgt ttggattttg cataagaaaa agaggttccc ccctttttat
    44641 gattttttac aaaagatttc cttggttcca taggcgacca gaacgagctc cagtgctaga
    44701 atcacttcaa ccaagactgg atttgtcaac cagcctactt gggaaagaga tggtgccaga
    44761 tcaaagaagg catgcaagcc ataggcagct aggagataaa tccatttctt ctggcgaaca
    44821 gcttggtaaa cccaaactgt caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa
    44881 ccaagcaaat aaatctgcca gaccgaaagt gactgaatgg tttttaacat attttcagac
    44941 agtaattgca taacctgtgg attctgagtt tgaactgtcg aaagaacgat gtaaagattg
    45001 agtaaactag taaggcctag aaaaatcaac tccaagccac catgccctag gccataggcc
    45061 aaggcatctg ccttttccaa acttctcttt ttctccaacc atttgaagaa aacaagacga
    45121 gccgtttcct caaaaaatgc tgccatggct aggccataga taatatagat aagcggatga
    45181 tcttgcaaga gggcaatact accgtctttt tgaggatgca aaatcaagat atgcaccagt
    45241 ttttccaaaa tctgcgaaga gacaaagaaa gcgactgcac ccaagcccaa gacagctaga
    45301 ttaatctggt atttcttttt ggcaaaccaa atgcttccta tcagaaaagc tagcaacagc
    45361 aacatggtaa taatgatatg aatggtcatt ttcttctcct attctgcctt ttcaatatct
    45421 tttttcatct cgtcaacatt gaacttagca aacaagtatt gacggtcttg gactgggaaa
    45481 cgttggtcca actggtcaac tgctcccacc tcgataatgc cttccttgat gacaaaatcc
    45541 atgacatgtc caccgaaggt caaatcatct gagatgaagt gcagatggta gcctgccaca
    45601 ctgaccccat ggaaaatctc aggcgtccag aaaccaacaa tagtccccac aacattgtca
    45661 cgactatatt ccggttgatg ggttgcgaca tcagcaaact tggtatcggg tgttgactta
    45721 ggaatcatac gcacatgcat atgtgaaaat tctccccgaa tcttgataga gcggaaaaga
    45781 ttttccccat cataataaga ctcaattcgt tcttccaatt ccttgtctgt catctcaaag
    45841 cgctggcgga aaatgacctc tgcctgatgc ggtactactg ctgcataagg aataagggca
    45901 tctggtgaca cttccacaat ttctggctgt tctcctgttc cttttgcctg ataagccttg
    45961 ccatctaaga caatcaattc cccatcaata gaatccaagg ttcccaaacc aaggtcacca
    46021 tgctctagca attctcccac tgtcatggta ccaccataaa ggccagccat caaggctcca
    46081 agagtattgt attgaaataa tttcactggt tcctgcacgt tcttatccat tcactttctt
    46141 gtctgttatt ctataaatgt tactcctaag tataccacat ttgcccctag atgtgaacga
    46201 gagaaacgct ctagacattg ccaagaagga aaaaaaaggg tacaatgtaa caaaatcaag
    46261 ggaggtctgg aatgaagaaa caaagcaagt acaaagaggt cgtttcctat ctgaaaaatg
    46321 gtatcgagtc tggacgattt ccgacgggta gtcgcctgcc ttctatccgt caactgagcc
    46381 ttgactttca ctgcagcaag gacaccattc aacgagccct gctggaatta cggcacgaac
    46441 aatacctcta tgccaagcct cagagtggct actatgtatt agaacaaggg caacatcaag
    46501 acctagaaat cgaggttacc gacgaacatg ccagtgccta tgacgatttc cgactctgtg
    46561 tcaatgaaac cttgattggc cgagaaaact acctcttcaa ctactatgac aatcaagaag
    46621 gattagaaga cctaagacag tccattcaca aactcctctt tgagcaagct ctctactgca
    46681 aggctaacca actagtactg acttctggaa cccaacaagc cttgtttatc ctctctcaaa
    46741 tatcctttcc tagacaagcc aaggaaatct tggtggaaca gccaacctac catcggatga
    46801 atcgcctctt gattgcacag gggctggact atcaaacgat tgaacgaggc attgatggga
    46861 ttgacttgga ggagctggaa ggccacttca aaacaggaaa aattaaattt ttctacacca
    46921 ttccccgatt tcactatcct ctgggacatt cctattctga gcaagacaaa cgatctattc
    46981 ttaacttagc tgccaagtat gatgtctata tcgtagagga cgattatctg ggtgatttgg
    47041 actccaagaa gggccaaacc ttccactatc ttgatacaga ggagcgtgtc atttatatca
    47101 agtccttctc gaccagcctt tttcctgccc ttcgtattac agcactcatt cttccaaatg
    47161 ctatcaaaga agcatttgtg gcctacaaaa atatcctaga ctacgacagc aacctcatta
    47221 tgcaaaaggc cctgtcactc tatattgaca gtcaattgtt tgaaaaaaat cgtttggctc
    47281 gcttgaccaa tcatgaatct taccaaaaac aaatcgagga aaggataact aaaacacctt
    47341 gtccccttcc tcattatccc ctacacgatg gtttattgct agacctgaga cagtatccta
    47401 aaatcgccag tctcaaacac agtcaactgg gcttggactt ctttgaagag gcctatttaa
    47461 gcacctgtcc ttatcaattt gccaaggtgt ccctagacaa tctggaaaat gttttaaact
    47521 atttaaaagc agaattggaa tgacatccaa ctctgctttt tcttattctt caacttcagt
    47581 tagacttgct gctttttcaa gatagctttg gtaagttcct tcatccttaa gtttttggat
    47641 gaccttgtct accacttctt tcaaatcatc actatttttt ctaagagcaa cggcattggc
    47701 gtcgccgtcc ttcatcttca agctgacagt tgcgacagct aagccagcgt ttttagcagc
    47761 ataactaagt gcaacaggct catccatatg aacagcatct acttttccag cctgcaattc
    47821 attgactgct tcacccatat tagttaggga agttaattga gcttttggca attgttcctt
    47881 gaccattgat tctggaacag tccctttttg ggctgcaata ttagcacttt ctaggctagt
    47941 taaatccttg tatttttcca catcagcctt acgaaccaag aaactaatct tgttttcata
    48001 gtatgggatt gaaaaatcaa agacttcttt tctctcgtca gtagcactaa ttcctgcaac
    48061 tgctaggtca gccttaccag tttgaagact ggtcaaaaca ttgtcaaaac tcatgcttga
    48121 gatttccaac ttaaccccaa gttcatcagc gatagcctga gccatgtcga tgtctgcacc
    48181 gactacctgg ttctttccat caaccaatga ttgaaattca aagggtgcat agtcaggact
    48241 ggttgccaca actaatttcc ccttttgctt aatggcctca acagctgact gagaaccatt
    48301 agaacctgac tggcaagcta ctaagaagaa acttgcaaga aaattacata aaacaaatat
    48361 ccattttttt gatttcataa ataaacaacc tctctgttaa ttttgtataa ttataacgat
    48421 atccaagtta cttgtcaagt gttttttaaa tttttatctc aaaaatattt tttcgttcaa
    48481 aaaaaggagc catcagttga tttcaagctc ctttttatac agaattaaac tattttataa
    48541 ttcgacaatc ttacctgttt caaagtagac aacccattca cagatatttt tagcatagtc
    48601 accgatacgc tccaagtagg aaataacttg gaaataatca cgacccgtaa caatggcttc
    48661 tggatttttc ttaatctctt cagtcgcaag gtcacggata gtttcaaaat agtggttaat
    48721 ttgctcatcc atggaggcca cccggtatgc atcgtcaaca gaaccattaa gataaagatc
    48781 aagtgctgct tcaacaacgc ttttaacttc acgtcccatt tttttaattt cttcctctac
    48841 agctgggatg cgttcttccc ccttcatacg gatggttgcc tgggcaatgg ctacagcgtg
    48901 atcccccata cgctccacat ctgatacagc cttaaggaca gtcaagactg tacgcaaatc
    48961 ttgagagact ggttgttgga gtgcgatcat ttcaaatgat ttcttttcca gtttcacttc
    49021 gtattcattt acttctgcat catcttcgat gacctctttt gccaggtcac ggtcatgcgt
    49081 gacaaaagca cgtaccgtac gattgatttg tgagagcact tcttgtccca tagcgtagaa
    49141 ctggttatgt aatttctcta aatcttcttc aaattgagat cgtaacatct ttcatctcct
    49201 tatccaaatt ttcctgtaat atagtcttcc gtttccttgt gttggggatc aaggaacatc
    49261 tgcttggtat cattaaattc aatcaaatct ccatctagga aaaatcctgt cttatcagag
    49321 atacgtgaag cttgctgcat ggaacgggtt accagaagca tggtgtactt gtcttttaga
    49381 ccatacaagg tttcctcaat tttaccagct gaaatcggat ccaaagccga agttggctca
    49441 tccaagagga tgattttagg actagttgcc aagacacggg ccacgcagac acgctgctgt
    49501 tgaccacctg acaatccaat agctgaatca tatagacgat ccttgacctc atcccagata
    49561 gaggcacctt gcaaggcttt ttctacggct tcatccagaa cctgcttatc cttaattcca
    49621 ttgatacgaa gcccgtagac aacattctca tagatagtca tagggaaagg attaggttgt
    49681 tggaaaacca ttccgatttc cttacgtaat tcaaccgtat ctgtacgcgg actgtagatg
    49741 ttgtggccat tgtacaccac ggatccagtt gtggtcacct ctggattgag atctcccatg
    49801 cggttgagag acttgaggag ggttgacttc cctgatccag atggaccaat caaggctgta
    49861 atttccttag gttggaaaga tagggaaaca ctattcaaag ccttcttttt attataataa
    49921 acggacaggt ctgatacctg taaaatcgca tctgtcatac ggtttccttt ctaaccaaag
    49981 tgaccagata catagtcatt ggtggactgt agcttggcat tttggaaaat agttgcagtc
    50041 ttgtcatact caatcaaatc acccaagtaa aagaagcctg tatagtcact tgcacgagca
    50101 gcctgctgca tattatgcgt tacaatgatg atggtaaagt ttttcttgag ctcaaacatg
    50161 gtctcttcta gttgcatggt cgcaatcgga tccaaggctg aggctggctc atccattaag
    50221 aggatatctg gcttaacaga gatggcacga gcgatacaga gacgttgttg ctgaccacct
    50281 gataaggtca aggctgactt gtggagatcg tctttaacct gatcccagag ggcagcctga
    50341 cgaagggagg tttctacgat ttcatctagg acttgcttat ccttaactcc agcacgttca
    50401 tgcgcaaagg taatattacg gtaaattgac ttagcaaatg gattggggcg ttgaaaaacc
    50461 attccaatgt gtttacgcat ttcataaacg ttgatttctg gacggttgac atcaattcca
    50521 cgatagagaa tctgcccagt tactttagca atatcaatgg tatcattcat gcgattgaga
    50581 ctgcgtaagt aggtagattt ccccgatccc gacggaccaa tcaaagctgt aattttattt
    50641 ctttcaaatt gcatatcaat ccccttaatg gattcatttt taccatagta aacatggaca
    50701 tccttagtag aaagggctac tttttcttca ggaaaggtaa ggatatgctt ctcatcccag
    50761 ttatatgttg acatggcttc tcctttaggc agcggttaat ttcttgtgta gatagcttcc
    50821 gaacttacga gctccaaagt taaaaatcag gataaagatc aggagcacag cggcagaacc
    50881 tgctgataca atggttccat ctggaatagt gccttcacta ttgactttcc agatatggac
    50941 agccaaggtt tctgcttgac ggaagataga gatggggcta gtcacactga ggatattcca
    51001 gttagaccag tcaagagctg gcgccgattg ccctgctgta tagatcagag ctgcagcttc
    51061 gccaaagata cgaccagatg ccaagacgac acccgttaca atacctggaa gcgcttctgg
    51121 aataacaaca tgaaccactg tctcccagcg agaaatccca agagccagac cagcctcacg
    51181 ttgggtatgg tgaacgtgtt tcaaactatc ctctacatta cgcgtcatct gaggcaagtt
    51241 aaagactgtc aaggccaagg cacctgaaat gattgaaaat ccatactcaa actggactac
    51301 aaagatcaag taaccaaaga gacccaccac cactgatggt aaagaggaca agatttcaat
    51361 acaagtccgc acaaagttgg taacaggacc ttttttagca tattcagcca agtaaatccc
    51421 agctcccata gaaagaggta cagaaataat caaggtaatg accaatagga aaaaggaatt
    51481 gtaaagctga atgccaatcc caccacctgc ttgaaaagca gaagaccttc cagtcaagaa
    51541 agaccaagag atatggggca agccccgaac caagatatag agaatcaagg aagccaagat
    51601 tgtcacaatg atgctagcaa tcgtatagag gacagctgtt gcaagtttat ctaatttctt
    51661 agcgcgcata atttttcttt cctctttctt tcgtaatcaa tttaatcaca ctgttaaaaa
    51721 ctaaactcat caagagcagt accaaggcca gtgaccagag aacattatta tttacagttc
    51781 ccatgacagt gttcccaatt cccatagtta atatagaagt taaagttgca gctggtgtgg
    51841 tcaaggaagt tgggataaca gctgagtttc cgacaaccat ctggatagct aaagcctcac
    51901 caaaggcacg cgccatccca aagaccactg cagtgaaaat accagaacgg gccgccttca
    51961 agatcacacg ccagatagtc tgccagcgag tggctcccat agcgaaactg gcttcacgat
    52021 aataacgagg aaccgcacgc aagctatccg ttgtcataaa ggttacggtc ggcaaaatca
    52081 tgacaaagag gacggaaatc cctgacaaaa tcccaaaacc agtcccacca aagacactgc
    52141 gaacaaaggg aacgacgact tgcaagccaa taaatccata cactactgaa ggaatcccaa
    52201 ccaggagttc aatagctggt tgcaaaatct tcgccccttt tggtgatact tcggtcataa
    52261 aaactgctgc accaatagca aagggtgttg cgataagggc tgagagaatg gtaacgataa
    52321 aggaacccaa aatcatagga agggcaccaa attctttact agaaggattc caagttcctc
    52381 ccaaaagaaa gtcaaagata ttcacaccat tgacaaagaa ggtcgacaag cctttttgcg
    52441 ctacgaaaac caaaatcatg gccacaagga tgactatcaa agaaagacag gcaaaggtca
    52501 aaccttttcc taatttctcc agacgagaat tctttgatgg aagcaacatt ttcttagcta
    52561 attcttcttg attcattatt gtctcccttc caacactgtc acagttccgg cagcatcttt
    52621 ttcaaccttc atttccttaa tcggaatata cttcaatcct ttgacaatcc cttcttgggt
    52681 ctcatccgag agaacaaaat tgagaaattc tgcagccaac tcattgggct gccccaatgt
    52741 atacatatgc tcataagacc acaagggcca attattgcta cttatatttt ctggacttaa
    52801 gtcatagcca ttcaacttca tgcttttgac cgaatcatct atataggtaa gagataaata
    52861 agagatagct cctggacttt ttgatacgat tgattttacc gctccatttg aatcctgctc
    52921 ctgactttgc atggcagact gaccttccat aatgacagta tcaaaggtag cacgagaacc
    52981 agagccggct gcccgattga taacagagat gggtaagtcc ttaccaccaa cctctttcca
    53041 attggttacc tcacctatga agatttgacg aagttgctct gtcgttaggt tatcaacatc
    53101 aacctcctta ttgacaatca gagccaagcc agctaccgcg accttgtggt caacaagagc
    53161 agaagcatca attccgtctt tttcctcagc aaatacatct gagtttccta tatcaactgc
    53221 cccagactga acctgggaca agcctgtacc agaacctccc ccttggacat tgaccgtttt
    53281 tccaacatgg atcgtgccaa attcatctgc cgctacttca accaagggtt gcaaggcagt
    53341 tgagccaaca gccgttatgg attctccacg atcaatccag ctagcacagc ctactaaaca
    53401 agccgtcagc caaaaagcga taagagacag agcaagcttt tttctttttt tcactgtttt
    53461 tctcctcgaa aataattatg aatactgtga attttttaag tagttcttta tgagttgacg
    53521 catgaattct taccaaattt ctgcgcaatt gattatttat ataatatagg ctatattact
    53581 ctttcctaac ctcctttttt catatgtgga taaaatctct tgtctatccc ttcccccatt
    53641 gtcacccatt atagtcattt cgtgtctctt tttccccttt ttaatgcaag ggaaattact
    53701 ctccttagat gataatccaa aagctagaaa ggtatctcaa acctctctac tctcccagac
    53761 tagtttacaa ctaaaaggaa aagattctat tttatgagaa atctagttta caagcggtaa
    53821 gaacgctaat aactaaactt cttgtactct ttgaaaatct cttcaaacca gtgttttgag
    53881 ctatctatgg ctagcttcct agtttgctct ttgattttca ttgagtagta aaactacatg
    53941 taatggcaat caagatatca agaatcatcc tactaaaaaa atccatactt tcactataac
    54001 atagaataag atatttgact agcattttca tttgaatctg aggccttttg gaaaataatt
    54061 tttcaaaaca tttccagtaa cctttgcaaa gcccaagcca ttgcctttaa ccaaaacttg
    54121 gtaccaacca tttggcagac tttctgccag ctgaacgatt tctccagccg catacttgac
    54181 aaacgcttct tggccaattt caaccgactg ttcgacctga ctcggtttca aggctaaacc
    54241 aagagcgaaa ctgggctcaa agcgtttctt cttaaaagtg cccagatgca gtccattgcg
    54301 agcaatcttg agcttcccta aatctggcaa aagttctggc aagagataaa gctggtctcc
    54361 aaaagtctgc aagatacccg gtagattgac cttcaaatgg ttttgggcaa attcctgcca
    54421 caaggcaact tgttcacggc tgaggttact cttacttgcc ttaaatttag gagctggatt
    54481 gttaccctta aactgtagat gggcaacaaa ctgaccctct cccttaaact gatgaggata
    54541 catccgagcc gtttctggca ggtcaatacc agctaccatt ccattgatat gctctactgg
    54601 caacaagtca aaatcatact cttccagcaa ccaattgaca atctcttcgt tttcctcggg
    54661 tgcccaggta caggtcgaat aaaccagatg accaccttca gctaacatgg tcactgcatc
    54721 ctccagaatt tctctttgca agctagcaca ttgactcgga taatctaagc tccaatagtc
    54781 catagcatca ggttgcttac gaaacattcc ttcaccagag caaggggcat caagaacgat
    54841 taagtcaaaa tagcctttaa agaccttgac caagcggtcg gcagattcat tggtcaccac
    54901 gacatttgtc gctccaaaac gctccatgtt ttcaaccaaa atcttagccc gtttgcttga
    54961 aatttcattg gaaacaagta gcccctcccc tgctagatag gctgccagtt gagttgattt
    55021 gccccccggt gcagcagcca agtccaagac cttcatacca ggactgggtt gggctacttg
    55081 agccaccatt tgagcagcag gttcttgcga ataaactaaa cctgtagcat gctcaggcga
    55141 tttccctgaa accttcccat agtggcccca aggggtttga gtaatggcat cagaaaagga
    55201 aagttgctct tcttttaagg gattgacccg aaaggccgaa accgcttcct cctcaaaaga
    55261 ggcaagaaaa tctcttgcct catctcctag tatctcttta tatttttcaa caaatccttc
    55321 tggaaattgc atttaagttc ttttcctttc gtaaatatag gactgaattt cctcctgcat
    55381 ctcaagaggc accatcatga ccggctgtct ggtttgaaaa tcaggagctt caccaaaaag
    55441 ggtcacaacc cgatagccca gactttcccc taaaatacta gctgcggcat aatcccatgg
    55501 ttgcagataa gtgagatagg tcaacaaacg ccctgacaaa atcttggcaa aactaatggc
    55561 cgcacttcca tagacacgaa caccaagaac cgatcggctc aaatcagcca gcccccattc
    55621 attggtttcc agcataccac tattccctgc aatgagaaaa tctccaagtg gtttagtttt
    55681 aaaaggagct agggacctat catttagaca aactggaaat tccccaccac cgtggtaaca
    55741 atcccctttg accacatcat aaatcagacc aaactgtccc tgaccatttt caaaataagc
    55801 catcataaca gcaaaatctt cctgctgggc tacaaaatta ttggtaccat caatgggatc
    55861 aatgacccaa accttgccct cttgaaccga ggctcgcaga caaccttctt cagcacaaat
    55921 cttatcctca ggataacggg acaaaatctc accaaccaag agttcctgaa cttctttgtc
    55981 cagtctggtc accaaatctg ttggagagga cttggtttca acacgcaagt cttcctgcat
    56041 atggtcaaga atgtactgac ctgctttctt aacaagctct ttagcaaatt caaatttact
    56101 ttccaagaga aatctttcct tccccttttt ctttggcagc ctgaactgct cggtaaagtg
    56161 aatagccact aaccgtttca aattctcgtc ccaaacgttt ctcttcgctc ttgcttggca
    56221 cgaccgcttt gaatccctta taggagtcca gtaacttttt agcctctact ttgccttcat
    56281 aggcagcttc aacatcatta aaaaaagaaa gcactgaagc aagttcttca gtgctccacg
    56341 acaaatctag tgggtaacta tactgtttgt tcattaacta ataccagctc tcattcttgc
    56401 ttcttttagt tcttgcttac gataactacg agggagaaaa gcacgaatct catcttcatt
    56461 aaaaccgatt tgcatacgct tggcatcaat aataattgga cgacgcaaaa gactaggata
    56521 ctgctcaatc aaatgaagca attccgatac cgaaatactc tctacatcaa tattcaattt
    56581 ttgaaaaatt tttgaacgag ttgaaatgat gtcatcagta ccattttcgg tcaaggaaag
    56641 gatgtgttgc aattcttttc ttgttaaagg actggtcata atattgtgtt ccacaaaggg
    56701 aaccttatgt ttttctaacc aggccttagc cttacgacat gatgtacagc tcggtgatag
    56761 aaatagtgta atcatgcttt tctcttctta tctatacttt gctacttcta ttatacaaaa
    56821 aaataaggcg cttgactagg gatttttaga aaaaaagcct attttttcag gaaaaatagg
    56881 ctgtttgcga acgattgaca caattggatt tggttaattc actcttaacg atggttttaa
//
LOCUS       DAVMAF010000004        53916 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMAF010000004 DAVMAF010000000
VERSION     DAVMAF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.004"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            2..74
                     /locus_tag="VYO97_000202"
     tRNA            2..74
                     /locus_tag="VYO97_000202"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VYO97_000203"
     tRNA            99..171
                     /locus_tag="VYO97_000203"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VYO97_000204"
     tRNA            177..258
                     /locus_tag="VYO97_000204"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VYO97_000205"
     tRNA            277..349
                     /locus_tag="VYO97_000205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VYO97_000206"
     tRNA            372..443
                     /locus_tag="VYO97_000206"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VYO97_000207"
     tRNA            451..536
                     /locus_tag="VYO97_000207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VYO97_000208"
     tRNA            546..619
                     /locus_tag="VYO97_000208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VYO97_000209"
     tRNA            635..708
                     /locus_tag="VYO97_000209"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VYO97_000210"
     tRNA            714..787
                     /locus_tag="VYO97_000210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VYO97_000211"
     tRNA            801..874
                     /locus_tag="VYO97_000211"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VYO97_000212"
     tRNA            885..974
                     /locus_tag="VYO97_000212"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VYO97_000213"
     tRNA            985..1058
                     /locus_tag="VYO97_000213"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VYO97_000214"
     tRNA            1062..1134
                     /locus_tag="VYO97_000214"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VYO97_000215"
     tRNA            1154..1224
                     /locus_tag="VYO97_000215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VYO97_000216"
     tRNA            1260..1333
                     /locus_tag="VYO97_000216"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VYO97_000217"
     tRNA            1340..1427
                     /locus_tag="VYO97_000217"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1560..1997)
                     /locus_tag="VYO97_000218"
     CDS             complement(1560..1997)
                     /locus_tag="VYO97_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1753091.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEA
                     TNNLIKLIKCNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(2204..3139)
                     /locus_tag="VYO97_000219"
     CDS             complement(2204..3139)
                     /locus_tag="VYO97_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1753092.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKA
                     KAASLFKATAPITRNELIIEIWRAFFETPSEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(3136..3996)
                     /locus_tag="VYO97_000220"
     CDS             complement(3136..3996)
                     /locus_tag="VYO97_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1753093.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVNLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            4104..6266
                     /locus_tag="VYO97_000221"
     CDS             4104..6266
                     /locus_tag="VYO97_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1753094.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRTTPLETRGHVAMMGNLGYELDLTSLSDEEKAEIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYKNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSKQYIFRRL"
     gene            6301..7560
                     /locus_tag="VYO97_000222"
     CDS             6301..7560
                     /locus_tag="VYO97_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1753095.1"
                     /translation="MKWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            7574..8440
                     /locus_tag="VYO97_000223"
     CDS             7574..8440
                     /locus_tag="VYO97_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1753096.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            8454..9287
                     /locus_tag="VYO97_000224"
     CDS             8454..9287
                     /locus_tag="VYO97_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1753097.1"
                     /translation="MKQDERKALIGKYILLILGSVLLLVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            9288..9368
                     /locus_tag="VYO97_000225"
                     /pseudo
     CDS             9288..9368
                     /locus_tag="VYO97_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9507..10949
                     /gene="gtfA"
                     /locus_tag="VYO97_000226"
     CDS             9507..10949
                     /gene="gtfA"
                     /locus_tag="VYO97_000226"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1753098.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGGDLIRLDAFAYAVKKLYTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKVLLNLFSFRNRSEAFDLEGTTEIETPTAQSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            11087..12280
                     /locus_tag="VYO97_000227"
     CDS             11087..12280
                     /locus_tag="VYO97_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1753099.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12967..14946
                     /locus_tag="VYO97_000228"
     CDS             12967..14946
                     /locus_tag="VYO97_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753100.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            15013..16509
                     /locus_tag="VYO97_000229"
     CDS             15013..16509
                     /locus_tag="VYO97_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753101.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16509..17435
                     /locus_tag="VYO97_000230"
     CDS             16509..17435
                     /locus_tag="VYO97_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753102.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINVVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17444..18511
                     /locus_tag="VYO97_000231"
     CDS             17444..18511
                     /locus_tag="VYO97_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753103.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18522..19448
                     /locus_tag="VYO97_000232"
     CDS             18522..19448
                     /locus_tag="VYO97_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753104.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(19523..20722)
                     /locus_tag="VYO97_000233"
                     /pseudo
     CDS             complement(19523..20722)
                     /locus_tag="VYO97_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20878..21588)
                     /gene="treR"
                     /locus_tag="VYO97_000234"
     CDS             complement(20878..21588)
                     /gene="treR"
                     /locus_tag="VYO97_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1753105.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21773..23740
                     /gene="treP"
                     /locus_tag="VYO97_000235"
     CDS             21773..23740
                     /gene="treP"
                     /locus_tag="VYO97_000235"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1753106.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELISPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            23918..25543
                     /gene="treC"
                     /locus_tag="VYO97_000236"
     CDS             23918..25543
                     /gene="treC"
                     /locus_tag="VYO97_000236"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1753107.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIKGPIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25799..26047
                     /locus_tag="VYO97_000237"
     CDS             25799..26047
                     /locus_tag="VYO97_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1753108.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26254..27048
                     /gene="racE"
                     /locus_tag="VYO97_000238"
     CDS             26254..27048
                     /gene="racE"
                     /locus_tag="VYO97_000238"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1753109.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27045..28016
                     /locus_tag="VYO97_000239"
     CDS             27045..28016
                     /locus_tag="VYO97_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1753110.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27992..28513
                     /locus_tag="VYO97_000240"
     CDS             27992..28513
                     /locus_tag="VYO97_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1753111.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28510..28971
                     /locus_tag="VYO97_000241"
     CDS             28510..28971
                     /locus_tag="VYO97_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1753112.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVLPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28962..29702
                     /gene="xerD"
                     /locus_tag="VYO97_000242"
     CDS             28962..29702
                     /gene="xerD"
                     /locus_tag="VYO97_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1753113.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29702..30430
                     /locus_tag="VYO97_000243"
     CDS             29702..30430
                     /locus_tag="VYO97_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1753114.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30449..31018
                     /locus_tag="VYO97_000244"
     CDS             30449..31018
                     /locus_tag="VYO97_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1753115.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31005..31727
                     /locus_tag="VYO97_000245"
     CDS             31005..31727
                     /locus_tag="VYO97_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1753116.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31727..31969
                     /gene="yidD"
                     /locus_tag="VYO97_000246"
     CDS             31727..31969
                     /gene="yidD"
                     /locus_tag="VYO97_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1753117.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(32194..33159)
                     /locus_tag="VYO97_000247"
     CDS             complement(32194..33159)
                     /locus_tag="VYO97_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753118.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEISFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33255..34007)
                     /locus_tag="VYO97_000248"
     CDS             complement(33255..34007)
                     /locus_tag="VYO97_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1753119.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIDSWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(34004..34960)
                     /locus_tag="VYO97_000249"
     CDS             complement(34004..34960)
                     /locus_tag="VYO97_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1753120.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILIGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(34950..35909)
                     /locus_tag="VYO97_000250"
     CDS             complement(34950..35909)
                     /locus_tag="VYO97_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753121.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKCYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36332..37033)
                     /locus_tag="VYO97_000251"
     CDS             complement(36332..37033)
                     /locus_tag="VYO97_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1753122.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKVYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKTFGDDYDTH
                     DGSCIRDYIYVLDLA"
     gene            37433..38497
                     /gene="pepA"
                     /locus_tag="VYO97_000252"
     CDS             37433..38497
                     /gene="pepA"
                     /locus_tag="VYO97_000252"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1753123.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38519..38815
                     /locus_tag="VYO97_000253"
     CDS             38519..38815
                     /locus_tag="VYO97_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1753124.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            39497..39940
                     /locus_tag="VYO97_000254"
     CDS             39497..39940
                     /locus_tag="VYO97_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1753125.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            39944..40708
                     /locus_tag="VYO97_000255"
     CDS             39944..40708
                     /locus_tag="VYO97_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1753126.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40723..41451
                     /locus_tag="VYO97_000256"
     CDS             40723..41451
                     /locus_tag="VYO97_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753127.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            41444..42964
                     /locus_tag="VYO97_000257"
     CDS             41444..42964
                     /locus_tag="VYO97_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1753128.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43309..44115
                     /locus_tag="VYO97_000258"
     CDS             43309..44115
                     /locus_tag="VYO97_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1753129.1"
                     /translation="MHTYLQKKIENIKATLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLPN"
     gene            complement(44281..44817)
                     /locus_tag="VYO97_000259"
     CDS             complement(44281..44817)
                     /locus_tag="VYO97_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1753130.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            44950..45840
                     /locus_tag="VYO97_000260"
     CDS             44950..45840
                     /locus_tag="VYO97_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1753131.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            46084..46437
                     /locus_tag="VYO97_000261"
     CDS             46084..46437
                     /locus_tag="VYO97_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1753132.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46438..47475
                     /locus_tag="VYO97_000262"
     CDS             46438..47475
                     /locus_tag="VYO97_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1753133.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47521..48528)
                     /gene="galR"
                     /locus_tag="VYO97_000263"
     CDS             complement(47521..48528)
                     /gene="galR"
                     /locus_tag="VYO97_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1753134.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVVEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGNQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48692..49870
                     /locus_tag="VYO97_000264"
     CDS             48692..49870
                     /locus_tag="VYO97_000264"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1753135.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYKVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49889..51370
                     /locus_tag="VYO97_000265"
     CDS             49889..51370
                     /locus_tag="VYO97_000265"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1753136.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEETVRLETIEDSQTAREILGTELMDLVTPCPSQVNSDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDETPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            51440..51844
                     /locus_tag="VYO97_000266"
     CDS             51440..51844
                     /locus_tag="VYO97_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1753137.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEDRQVRI"
     gene            52001..52855
                     /locus_tag="VYO97_000267"
     CDS             52001..52855
                     /locus_tag="VYO97_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV1753138.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            52881..53651
                     /locus_tag="VYO97_000268"
     CDS             52881..53651
                     /locus_tag="VYO97_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1753139.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            53638..>53916
                     /locus_tag="VYO97_000269"
     CDS             53638..>53916
                     /locus_tag="VYO97_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1753140.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFY"
BASE COUNT        15569 a         9872 c        12178 g        16297 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt gagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttatt tttagagaca ataaagcttc agatccgtaa aacttaattg
     1501 aattactaat ctgaagttat ttttatggct ctttgtcaac tgtagtgggt tgaagtcagc
     1561 taagctcgag aaaggacaaa tttcgtcctt tcttttttga tgttcaaagc gataaaaatc
     1621 cgttttttaa agttttcaaa gtttcgaaaa ccaaaggcat tgcacttgat aagtttgatg
     1681 agattattgg tcgcttccag tttggcgtta gaatagtgta gttgaagggc gttgacaatc
     1741 ttttctttat ctttgaggaa ggttttaaag acagtctgaa aaataggatg aacctgctta
     1801 agattgtcct caataagtcc gaaaaatttc tctggttcct ttttcttcat tagacttcca
     1861 caatcagggc aagatggagc ctcataatcc agcttagcga taatttcttt gtgggtatcc
     1921 atattgatga tatctagaat cttgatgttt gggtctttaa tatcgagcag ttttgtgata
     1981 aaatgtaatt gttccatatg aatctttcta atgatggttt tgtcgctttt cattataggt
     2041 catatgggac tttttttcta caacaaaata ggctccataa tatctataag ggatttaccc
     2101 actacaaata ttatagagcc aataaaactt cagatccgta aaacttaatt gaattactaa
     2161 tctgaagtta tttttatatc gtttatatta taattgtgtt attttacttc caactattga
     2221 gagaaatttc gccactattt agccagattt cttttccgtt atcacattga actaaaagtt
     2281 ttccattctc tgagatgtct ttagcaagtc ccttgtagtc tttttgctct agtgtgaaag
     2341 tgacttcttt tcctagaatg aatgactgtt ttttgtatag gtataatagc tcttctgatg
     2401 gtgtttcgaa gaaagcacgc cagatttcta tgatcaattc attccttgtt ataggagctg
     2461 tagctttaaa taagctggca gcttttgctt ttaattcctg agggaagtct ttaatagtga
     2521 aattgatacc tactccaata atgatatctg tgactaagcc agtttctaca gaggtcattg
     2581 cttcagtaag gattcctcca attttatgat tgtttagata gatatcattg acccatttta
     2641 tgtcgacatc tattaaagtt aggttcttaa tggctttgta gacagctcca gctacaagta
     2701 gtgtgtagga tggtaatttg tcatagggga gatttggttt aagatggagt gtcatataaa
     2761 taccaccttg tggtgagtag aaggaacgtt gaaaacggcc tcggcctgct gtttgatagg
     2821 aagctagata gagggtattt gcttcatggc ctaaatcaat tgcttctttt gcatctagtt
     2881 gtgttgattt tgtttcgggt ttaaagctga ctttaattgg aagattttct tctagaatct
     2941 ctggaagaat aaggtcacca ttcattagtt tatatcctct atttttgata ctatcaattt
     3001 caatgccttc ttgttctagt cgcttgatag ctttccaaat tgatgttcgg cttagggata
     3061 gtttttctgc gattttttct ccgctgatat agtcggtttc tttagatagg atttggtaga
     3121 cagcttggta ggatttcata atgttgcctt tctcactagt tggtattgag agtattcttt
     3181 tcttgtatga cttggagtct gattaaagta ttgtttataa gctttcgaaa aatggagtgg
     3241 atctgaaaaa cctaccgagt atgcaattac cttgatggac tcttgggtat tttcgagaag
     3301 ttgtctagct cggtgcattc gaacgtagag taggtattct ttgggtgata aggtattaaa
     3361 ttctttgaat acgcttgata agtagcttct gtgaacggat agttcttttg ctaaatcttg
     3421 aattgtaagt gattgaggat agtggctatc aattaatcgt ttgcattcaa gatagagttg
     3481 gtgggttgat gaaatattct tttttttctg attgggagca atagttccca gatgaaacat
     3541 cagttcatga agttgtccca tgatatggag ttgagctaat tcacttgatt ttgtaatctg
     3601 agcgaagcgg acaatgtctg agatgagttt tgcagtagtc tgggtatgac aagtttcaga
     3661 ttggatgaga taggattgat cagaaatttg agaaagagca aaataatcag gggatttccc
     3721 tccagtgatt cctaaccagt agtaggccca aggttcttta ctatctgctt gataaaaggt
     3781 tagttcctct ggttttaata gaaagaaatc tccttctttt aaattaacaa ttttaccctt
     3841 gtaatgaaat tttccttgtc ctttagtaat gtaatgtagg acgtatgtat cacgaatggc
     3901 tggaccaaaa gagtaattag gtgtgcattc ctcatatcca taaaagctta gggcaaggtc
     3961 gattgttcca gtctggtatt ctgaaaaaac taacatgtgc tacctcctac ctaacatttt
     4021 accatattct tgagacattt ttctatttta gaaagcgatt tcaggtgata aaatataatc
     4081 aataaagtga tgaggtgaag taaatgggag ttaggataga gaataatcta ttttatgttg
     4141 agagtaaaaa tctaagtttg attattgaaa atcgaaatgg ctacttactt ttgaaacatt
     4201 taggaaagac tattaagaac tataaaggtt ccaatagtgt ttatgaacga gaccatgcct
     4261 tttcaggaaa tccaacggct actaatcgaa cctttagttt agatactcag cgacagattt
     4321 ttggacaaca tggcttagga gattttagga aaccaaccat acaggttcag catagtgtaa
     4381 ctgaagtaac agactttcga tttgtagaag caaagatttt aaaaggtcag aatggtccac
     4441 agggcttacc ttctccacat agcatggacg atacagagac tcttgtctta atgttagaag
     4501 attctaaggc tcaacttagt ctgactttgt attatactac ttttaataat gatgcgacta
     4561 ttgctagcta cagtaaatta gataataata gtaatcagga agttgtcatc cataaagatt
     4621 tttcttttat ggctgatttt ccagctgcag attacgaaat agtaactctg cagggtgctt
     4681 atgctcgtga aaagactgtt agacgtcaac aggtagaaca aggaatcttt tcgattagct
     4741 caaaccgagg tgcttctggt catgctcaaa caccagctct tctactatgc gaacaaggag
     4801 tcacagagga tgctgggaat gtgtttgcta ttcaactaat gtatagtggc aactttgaag
     4861 cttttgttca aaaaaatcaa ttgaatgaag ttcgggtggc tattggcatt aatccagaaa
     4921 acttttcttg gaagttagcc cctgaggaat actttgaaac accggtagct ttagtgactc
     4981 attcagatca gggattaact ggtattagtc atgaaagtca gaattttgta ctgaagcaca
     5041 ttattctaag tgaattttct aaaaaagaac gtccaattct aatcaataac tgggaagcta
     5101 cttactttga ctttcagaga gaaaaactgt tagagttagc agatgaagct aagaaagttg
     5161 gcattgaact ttttgtatta gatgatggtt ggtttggcaa tcgttttgat gataatcgtg
     5221 ctttaggtga ttgggttgtt aatgaggaaa aactgggtgg aagtctagaa agtctgattt
     5281 cagctatcca tgaaagaggt ttgcagtttg gactttggtt agaacccgaa atgatttctg
     5341 tagatagtga tttgtatcgt caacatcctg actgggctat tcaggttcct gattatgagc
     5401 atacttattc tcggaatcaa ttagtactta atcttgccaa tcctcaggta gtagaatact
     5461 tgaaaagtgt cttagatcaa ctcctatctt atcatgagat tgattacatt aaatgggata
     5521 tgaaccgcaa tatcactaag ctagggaatg gattaactta tctagagaca cagatgcaat
     5581 ctcatcagta catgctgggg ctttacgaac tcgtttctta tctgacagag aagcacagcc
     5641 atattctctt tgagtcctgc tctggtggtg gtggacgaaa tgatcttggt atgatgcgct
     5701 atttcccaca agtctgggct agtgataata cagatgctat tgcacgttta ccaattcaat
     5761 acggttcatc ctatctctat ccaaccattt ctatgggggc tcatgtgtca gcagtaccga
     5821 atcatcagat ggggcgaacg acaccattag aaacacgtgg acatgtagca atgatgggaa
     5881 atttgggcta tgagcttgat ttgacaagtt tatcagatga agagaaagct gagattgcta
     5941 atcaggtgaa cttgtataaa gaattacgac cagtagttca gttaggacaa cagtatagac
     6001 taattaatcc tgatactgca tccaatgaag ctgctgtaca atttaattac aaaaatcaaa
     6061 cgattgtaac ctacgttcgc gttttgtcag ttgtagagac catggaaaca actttaaagt
     6121 taaaagattt ggatgaagag ggactatatg aattacagga aaatggcgaa gtttactcag
     6181 gtgcagaact catgtatgcg ggtttaactg ttattttatc ccaaggagat tttttgagta
     6241 aacagtatat ttttagaaga ctataattaa ggtgtataaa tttataaagg aggctttcca
     6301 atgaaatggt ataaaaaaat cggacttctt gcaactacag gtttagcttt ggttgggctc
     6361 ggcgcttgct ccaactatgg taaatctgcg gatggcacag tgaccatcga gtatttcaac
     6421 cagaaaaaag aaatgaccaa aaccttggaa gaaatcactc gtgattttga gaaggaaaac
     6481 cctaagatca aggtcaaagt cgtcaatgta ccaaatgctg gtgaagtatt gaagacacgc
     6541 gttctcgcag gagatgtgcc tgatgtggtc aatatttacc cacagtccat cgaactgcaa
     6601 gaatgggcaa aagcaggtgt ctttgaagat ttgagcaaca aagactacct gaaacgcgtg
     6661 aaaaatggct acgctgaaaa atatgctgta aacgaaaaag tttacaacgt tccttttaca
     6721 gctaatgctt atggaattta ctacaacaaa gataaattcg aagaactggg cttgaaggtt
     6781 cctgaaacct gggatgaatt tgaacagtta gtcaaagata tcgttgctaa aggacaaaca
     6841 ccatttggaa ttgcaggtgc agatgcttgg acactcaatg gttacaatca attagccttt
     6901 gcgacagcaa caggtggagg aaaagaagca aatcaatacc ttcgttattc tcaaccaaat
     6961 gccattaaat tgtcggatcc gattatgaaa gatgatatca aggtcatgga catccttcgc
     7021 atcaatggct ctaagcaaaa gaactgggaa ggtgctggct ataccgatgt tatcggagcc
     7081 ttcgcacgtg gggatgtcct catgacacca aatgggtctt gggcgatcac agcgattaat
     7141 gaacaaaaac cgaactttaa gattgggacc ttcatgattc caggaaaaga aaaaggacaa
     7201 agcttaaccg ttggtgcggg agacttggca tggtctatct cagccaccac caaacatcca
     7261 aaagaagcca atgcctttgt ggaatatatg acccgtccag aagtcatgca aaaatactac
     7321 gatgtggacg gatctccaac agcgatcgaa ggggtcaaac aagcaggaga agattcaccg
     7381 cttgctggta tgaccgaata tgcctttacg gatcgtcact tggtctggtt gcaacaatac
     7441 tggaccagtg aagcagactt ccataccttg accatgaact atgtcttgac cggtgataaa
     7501 caaggcatgg tcaatgattt gaatgccttc tttaacccga tgaaagcgga tgtggattag
     7561 gagtagagag actatgaaaa aagtattaca aaaatattgg gcatgggctt ttgtggtcat
     7621 ccccctcttg ttacaagcaa ttttcttcta tgtgccgatg tttcaaggag ccttttacag
     7681 ttttaccaac tggacaggat tgacttacaa ctacaaattt gtcggcttaa acaactttaa
     7741 gctcctcttc atggatccaa aattcatgaa tgcgattggc tttaccgcaa tcattacgat
     7801 tgccatggtg gttggtgaga ttgcactcgg gatcttcatt gcgcgtgtct tgaactctaa
     7861 aatcaaaggc caaaccttct tccgtgcttg gttcttcttc ccagctgttt tatctggttt
     7921 gacagtggct ttgatcttca agcaagtctt caactacggt cttccagcga ttgggaatgc
     7981 ccttcatatt gaatttctcc aaaccagtct tttagggact aagtggggag caatctttgc
     8041 ggctgtcttt gtccttcttt ggcaaggggt ggctatgccc atcatcatct tcctagctgg
     8101 tttgcaatct attccaactg agattacaga ggcagcaagg attgatggtg cgactagcaa
     8161 gcaagttttc tggaacattg aattgcctta cttgctacca agtgtctcta tagtctttat
     8221 cctagcccta aaaggtgggc tgactgcctt tgaccaagtc tttgccatga ccggtggtgg
     8281 tccaaacaat gccacaacct cacttgggct cttggtttat aactatgcct ttaaaaacaa
     8341 ccaattcggt tatgccaatg ccattgccgt aatcttgttc ttcttaattg tagtgatttc
     8401 gatcatccaa ttgagagtat ctaagaaatt tgaaatttaa gaggagaagc atgatgaaac
     8461 aagatgaaag aaaagccctg attggcaaat acattctatt gattctagga tcggttctgc
     8521 ttttagtgcc gctccttgct accctcttta gttccttcaa acccactaag gatattgtag
     8581 ataatttctt tggctttcca accaacttca catgggacaa ctttagccgt ctcttagctg
     8641 atgggattgg aggctattat tggaactctg tcgtcatcac tgtcttgtct ttacttgcag
     8701 taatgatctt tatccctatg gcagcctact ccatcgctcg caatatgagt aaaagaaaag
     8761 cctttaccat catgtatacc ctcttaatcc tcggaatctt cgtacctttc caagtcatca
     8821 tgattccgat tacggttatg atgagtaaac tcggtttggc taataccttt ggtttgatct
     8881 tgctctactt gacctatgcg attccacaga ccctctttct ctatgttggc tatatcaaaa
     8941 tctcgattcc agaaagtctg gatgaagcag cagagatcga tggggctaat caatttacaa
     9001 cctatttccg catcatcttc ccaatgatga aaccgatgca tgcgacaacc atgatcatca
     9061 atgccctttg gttctggaat gacttcatgt tgccactcct tgtcttgaac cgggattcca
     9121 aaatgtggac tctgcctttg ttccaataca actacgcagg ccaatatttc aacgactacg
     9181 gaccaagctt tgcctcttac gtggtcggca ttatcagtat caccattgtc tatctcttct
     9241 tccaacgcca tatcatttca ggaatgagca acggggcagt gaagtaatac tcaatgaaaa
     9301 tcaaagagca aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtgg
     9361 atgaaactga cgaagtcagt aaccatacct acggtaaggc gacgctgacg tggtttgaat
     9421 ttgattttcg aagagtataa catgaatttg atttacgaag ccagattata tgagacttgg
     9481 cttcaattag aaaaaggaga gtaatgatgc caattcaaaa taaaaccatg ttgattacct
     9541 attctgatag ccttggaaat aatcttaaag acttatatga taatttggaa gagcattttg
     9601 gagatgctat tggaggagtt caccttttac catttttccc atcaacaggt gatcgtggat
     9661 ttgcgccagt tgactacgac gaagtggact cagcttttgg tgattgggag gatgttaagc
     9721 gtttaggtga gagatattat cttatgtttg attttatgat taatcatatt tctcgtcaat
     9781 ccaagtatta taaggactat caagaaaaac atgaagccag tgaatttaaa gatctctttt
     9841 taaactggga taagttttgg ccagaaaacc gtccgacaca gtctgatgta gatttaattt
     9901 acaagcgtaa ggatcgtgca ccaaagcaag agattgtgtt tgaagatggt tcagtggaac
     9961 atttgtggaa tacttttggt gaggagcaga ttgatcttga tgtgaccaaa gaagtaacta
    10021 tggaatttat ccgtaagacc attcagcact tggcaagtaa tgggggtgat ttgattcgtc
    10081 tagatgcctt tgcttatgca gtgaagaaat tgtatactaa tgatttcttt gtggaaccag
    10141 atatttggga tttattggac aaagttcgag atatcgctgc tgagtatggg acagagcttt
    10201 tacctgagat tcatgaacac tattcgattc agtttaaaat agcagaccat gattactatg
    10261 tttatgattt tgctcttcca atggtgacac tttatactct ttacagttcc agaacagagc
    10321 gtttggctaa gtggttaaag atgagcccga tgaagcaatt tacgacgcta gatacccatg
    10381 atgggattgg agtagtagat gtcaaggata tcctgaccga tgaggagatt gactatgctt
    10441 caaatgaact ctataaggtt ggagccaatg tcaaacgtaa gtactctagt gccgagtata
    10501 acaacttaga tatctaccaa atcaattcaa cctactattc agcgcttgga gatgatgatg
    10561 tcaagtattt tctcgctcgt ctaattcaag cttttgcccc aggtattcct caggtttact
    10621 atgtgggtct attagcaggc aagaatgact tgaaattatt agaagaaact aaagaaggtc
    10681 gaaatattaa tcgtcattac tatagcaacg aggaaatagc aaaagaagtg caacgatctg
    10741 ttgtgaaggt ccttctcaat ctattttctt tccgtaaccg ttcagaagcc tttgatctag
    10801 aagggactac tgagatagag acaccaacag ctcagagcat tgtaatcaaa cgtcaaaata
    10861 aagataagtc cgtaacagca gtagcagaaa ttgatttgca aaatcagact tatcgggtaa
    10921 ttgagaatgg agttgaagta acattttgaa gccttgatat ggtggataaa atagggtttt
    10981 tattttggaa aacgtttcct ttgttttcaa attgctaaaa aagtggtaca atataggata
    11041 gcttactatt atctgaatca gctgatttgg agagaaagga ttcattttga aatcaatagg
    11101 ctttattgaa aagctgaagg ggttgtctag taaagagctg attttattgg gaattatcct
    11161 gagtatcttt ttaccctttt atctttttgt agttgtactc tgtttatata ttatcagttt
    11221 gatttttaca ggagacatga aaagtattct tcagaaaatg ggggagcatc cgatgctgct
    11281 tctttttctt agctatagta ctgttatatc cattcttgca caaaattgga tgggtcttgt
    11341 ggcttcagta ggaatgtttc tatttactat tttctttttg cactatcagt cgattttatc
    11401 ccataaattc tttcgattga ttttgcagtt cgtcttgttt ggtagtgtct tgtcagctgc
    11461 ttttgccagt ttagaacatt tccaaattgt gaagaaattt aactatgctt ttctttcacc
    11521 caatatgcag gtgtggcatc agaaccgggc agaagtgacc ttctttaatc ctaattatta
    11581 tggaattatt tgttgtttct gtattatgat tgctttctat ctgtttacaa cgaccaagtt
    11641 gaattggttg aaagtattct gtgtgattgc aggctttgtt aatctctttg gtttgaactt
    11701 tactcaaaat cgaactgcct ttcctgctat tatcgctgga gcaattatct atctctttac
    11761 gactattaaa aactggaagg ccttttggct tagtattggg gtcttcgcga ttggtttgag
    11821 tttcctcttt tctagtgatt tgggagttcg aatgggtact ttagactctt ctatggaaga
    11881 acgcatttct atctgggatg ctgggatggc cttgtttaag caaaatcctt tttggggtga
    11941 agggccattg acctatatgc actcttatcc tcggatacat gctccttatc atgaacatgc
    12001 ccacagtctt tatattgata cgattctgag ttacggaatt gtgggtacca ttttattagt
    12061 tttgtcttct gttgctcctg ttcgcttgat gatggatatg agtcaggagt cggggaaacg
    12121 tccgattatc ggcctttatc tatctttcct tacagtggtt gctgtgcacg gaatttttga
    12181 cttggctctc ttctggattc agtcaggctt tattttcttg ctagttatgt gcagtattcc
    12241 attggagcat cgaatgttgg tatcggacat gacggattaa gttttataag tttgaaatct
    12301 tctaccttgg gtaggagatt tttttatacg atgggcatgt cgtgcatctg aggtgtaagt
    12361 cctctgtagg gatccactgc cggtgaatac aatagcgctc cccaagcctg actatcgtga
    12421 ggtagcggat taaaatggtc tggggataga tcgttttaag tctgacgcag gaaataagaa
    12481 ttgtcagaga aagcaatagt gaaatcgtag gctatgaata gaaacgtgat agcgaggagc
    12541 atatagtgga taaggtagcg agaaatttta aagtataaaa gatagtctta atctatatat
    12601 gtaaatcaca aaagtaattg aattcggaat aaggttggtg tgaaaagaca tagtaaactg
    12661 aatcttgaat actagaatga tacttctaca atattgttat aatttaattt gaattccaaa
    12721 atcacattgt tccgtccgta tttcatttta ctgtatgtct tcctaaactc caaagtttct
    12781 gcgtcaaatt tcctattttc actgtagtct ttgacacttc gaacgacaat ttgaatcata
    12841 tattcaaaaa aaaatgaaaa atttgtgcca acaagcctaa atattctgaa aatttgtttg
    12901 atttttaatg gataatgtga tataatggtt gtcaatctat aattcacata aacagaggag
    12961 tagaaaatga aaaaaaatcg tgtatttgct acagcaggtc ttgttttatt agcagcaggt
    13021 gtacttgcag catgcagttc ttcaaaatca tctgactcat cagcccctaa agcttatggc
    13081 tatgtttata cagcagaccc agaaaccttg gactacctga tttcaagtaa aaatagtaca
    13141 acagtagtga cttcaaatgg gattgatggt ttattcacta acgataatta cggtaatctt
    13201 gctcctgcag ttgcagagga ttgggaagtc tctaaggatg gtttgaccta cacttataag
    13261 attcgtaaag gggttaaatg gtttacctct gatggagaag aatatgcaga ggtgacggct
    13321 aaagatttcg tgaacggttt aaaacacgca gcagataaaa aatcagaagc tatgtattta
    13381 gctgaaaatt cggttaaagg cttggcagat tatctatcag gaacttcaac agatttttca
    13441 acagttggtg tcaaggcggt tgatgattat acgttacaat acactttgaa ccagcctgaa
    13501 ccgttctgga actctaagtt gacctattct attttctggc ctctgaatga agaattcgaa
    13561 acatcaaaag gaagcgattt tgctaaacca acagatccga catccttgct ttataatggt
    13621 ccattcttgt tgaaagggtt gactgcaaaa tcttctgtag agtttgtaaa aaatgagcaa
    13681 tattgggata aagaaaatgt ccacctagat actatcaatc tagcttacta tgatggatca
    13741 gatcaggagt cgctagagcg taacttcact agtggagctt atagttatgc ccgtctttac
    13801 cctaccagct ccaactattc taaggttgca gaagaataca aggacaatat ctattacaca
    13861 caatcaggct ctgggattgc tggtctgggt gtgaatattg atcgccaaag ttacaactat
    13921 acttctaaaa ctacagattc agagaaagta gctactaaga aggcattgct taacaaagat
    13981 ttccgtcaag ccttgaattt tgctcttgat cgctcagctt actcagctca aatcaatggt
    14041 aaagatggag cagctttagc agttcgtaat ttatttgtaa aaccagactt tgtttcagct
    14101 ggtgagaaga cctttggtga tttagtcgct gctcaacttc ctgcttatgg tgatgagtgg
    14161 aaaggtgtga atttagctga tgggcaggat ggtttattca atgctgacaa ggccaaggca
    14221 gagtttgcga aagctaagaa agctttagaa gcagacggcg ttcagtttcc tattcatctg
    14281 gacgttccag tagaccaagc atcaaaaaac tacatatctc gtattcagtc ctttaaacaa
    14341 tctgtagaaa cagttcttgg tgttgaaaat gtcgttgttg atattcaaca aatgacaagt
    14401 gatgaattcc ttaatattac ttactatgct gccaatgctt catctgagga ttgggatgta
    14461 tcgggaggag tttcatgggg gccagactat caagacccat ctacttacct ggatatttta
    14521 aaaacaacta gcagtgaaac tacaaaaaca tatttaggat ttgataatcc aaatagccct
    14581 tcagtagttc aagttggttt gaaagaatac gataaattag ttgatgaagc tgccaaagag
    14641 acaagcgact tgaatgtccg ttatgaaaaa tatgcagcgg ctcaagcatg gttgacagat
    14701 agttcactct ttattcctgc tatggcttct tctggtgcag caccagtgct ttcacgaatt
    14761 gttccattta ctggagcttc tgcgcaaaca ggctctaagg ggtcagatgt ttacttcaaa
    14821 tatttgaaat tacaagataa agtggtgact aaggaagagt atgaaaaagc tcgtgaaaaa
    14881 tggttgaaag aaaaagctga atcaaatgag aaagctcaaa aagaattggc aagtcatgtg
    14941 aagtaaataa tttctaatag aactttctct ccatgaggag aaggttcttt tgggattttt
    15001 aaaggaaata atatgaaaaa atatattttt atgcgtgttt tgcggtcatt ggtttcgatt
    15061 ttcttagtaa cgactttgac ctacacgatt atctatacct tggttcctcg aaaattgatt
    15121 ttcaagcagg atcctaacta taataaaatt gcgacaacgg ctgataaacg tgataactat
    15181 gaaaatactg tgtttgagcg tatgggctac attgagtatt acgatactaa agagttgcaa
    15241 gaaaaggcaa gtagcatgga ttcttctgta acagtagaag caaatgcgac caataaagct
    15301 atttatgaaa agtacatcaa tcaattaggt catggttgga ctttgggaga atttactgaa
    15361 agtggtcaat tctatgctac tcgtgaaatt ccaatttttg aacgtgtttt tcacttctat
    15421 gctaacttga ttgacattga ccatacaaat aaaatccaag accctgaaaa tccagacttg
    15481 aaacgctacc ttcgttttga aaatgatcca gctatcggat ggtcattggt cggttcagga
    15541 actaaacata aatatctctt gtactttaac agtcagttcc catttgttca tcaaaacttt
    15601 gtgaacttga atttaggtga ctcttaccca acctatgcta atacaccagt tcttcaggtt
    15661 attactcaag gtcaaggaca aaccaaaact gcccaagttc agttcccaac aggtaagaaa
    15721 acgtcttctg taaatattta ctcaagaacc tacaagtcac ctagtcaggc tgactctcgt
    15781 gaagtagcta gctatgggaa agatgatcct tatacagcga ctgaaagtaa ttaccaatat
    15841 ccatctatga ttgtcagctc tgctattact ggtttgattg gtttggttct tgcctatgct
    15901 cttgccgtgc cacttggttc agccatggct cgtttcaaga acacttggat tgatagcctc
    15961 tcaacaggga ctttgacctt cttgcttgct cttccaacga ttgccttggt ttacatcgtt
    16021 cgattgattg gatcatctat tgcccttcca gattcattcc ctatcttggg agctggagat
    16081 tggcgttctt acgttttacc agcagtcatc cttggtttgt tgggtgctcc tgttacagcc
    16141 atttggattc gtcgttacat gattgacttg caatctcaag actttgttcg tttcgctcgt
    16201 gcaaaaggtt tgtctgaaaa agaaatttca aacaaacaca tctttaaaaa tgccatggtt
    16261 ccgctggttt caggaattcc tggtgccatt attggggtta tcggtggtgc aacccttact
    16321 gaaacagtct tcgccttccc aggtatgggt aaaatgttga ttgactctgt aaaagcatct
    16381 aataactcta tggtcgttgg tcttgtcttc atctttacat gtatttctat cttctcacgt
    16441 cttttgggag atatttggat gactattatt gacccacgta ttaaattgac tgagaaagga
    16501 ggcaaataat gtctacaatc gataaagaaa aatttcagtt tgtaaaacgt gacgattttg
    16561 cctctgaaac tattgatgcg ccagcatatt cttactggaa atcagtgttt aaacaattta
    16621 tgaagaaaaa atcaactgta gtcatgttgg gaatcttggt agccatcatt ttgataagtt
    16681 tcatctaccc aatgttttct aagtttgatt tcaatgatgt cagcaaggta aacgacttta
    16741 gtgttcgtta tatcaagcca aatgcggagc attggttcgg tactgacagt aacggtaaat
    16801 cgctctttga cggtgtctgg ttcggagctc gtaactccat cctcatttct gtgattgcga
    16861 cagtgattaa cgtggttatc ggtgtttttg tcggtggtat ttggggtatt tcaaaatcag
    16921 ttgaccgtgt catgatggaa gtttacaacg tcatctcaaa catcccacct cttttgattg
    16981 ttattgtctt gacttactca atcggagctg gattctggaa tctgattttt gccatgagcg
    17041 taacaacatg gattggtatt gccttcatga tccgtgtgca aatcttgcgc tatcgtgact
    17101 tggaatacaa cttggcgtca cgtactttgg gaacaccaac cttgaagatt gttgccaaaa
    17161 atatcatgcc tcaattggta tctgttattg tgacaaccat gactcaaatg cttccaagct
    17221 ttatctcata cgaagccttc ttgtctttct tcggtcttgg attaccgatt acagtgccaa
    17281 gtttgggtcg tttgatttcg gattattcac aaaacgtaac aaccaatgct tacttgttct
    17341 ggattccatt gacaaccctt gtcttggtat ccttgtccct tttcgtagtt ggtcaaaact
    17401 tagcggatgc tagtgatcca cgtacacata gataggagta gaaatgacaa aagaaaaaaa
    17461 tgtaattttg actgctcgcg atattgtcgt ggaatttgac gttcgtgaca aagtattgac
    17521 agccattcgc ggcgtttccc ttgaactagt cgaaggagaa gtattagcct tggtaggtga
    17581 gtcaggatca ggtaaatctg ttttgacaaa gaccttcaca ggtatgctcg aagaaaatgg
    17641 tcgtattgcc caaggtagta ttgactaccg tggtcaggac ttgacagctt tatcttctca
    17701 caaggattgg gaacaaattc gtggtgctaa gattgcgact atcttccagg acccaatgac
    17761 tagtttggac cccattaaaa caattggtag tcagattaca gaagttattg taaaacacca
    17821 aggaaaaaca gctaaagaag cgaaagaatt ggccattgac tacatgaata aggttggcat
    17881 tccagacgca gatagacgtt ttaatgaata cccattccaa tattctggag gaatgcgtca
    17941 acgtatcgtt attgcgattg cccttgcctg ccgacctgat gtcttgatct gtgatgagcc
    18001 aacaactgcc ttggatgtaa ctattcaagc tcagattatt gatttgctaa aatctttaca
    18061 aaacgagtat catttcacaa caatctttat tacccacgac cttggtgtgg tggcaagtat
    18121 tgcggataag gtagcggtta tgtatgcagg agaaatcgtt gagtatggaa cggttgagga
    18181 agtcttctat gacccttgcc atccatatac atggagtctc ttgtctagct tgcctcagct
    18241 tgctgatgat aaaggggatc tttactcaat cccaggaaca cctccgtcac tttatactga
    18301 cctgaaaggg gatgcttttg ccttgcgttc tgactacgca atgcagattg acttcgaaca
    18361 aaaagctcct caattctcag tatcagagac acattgggct aaaacttggc ttcttcatga
    18421 ggatgctcca aaagtagaaa aaccagctgt gattgcaaat ctccatgata agatccgtga
    18481 aaaaatggga tttgcccatc tggctgacta ggaggaagga aatgtctgaa aaattagtag
    18541 aaattaaaga tttagaaatt tccttcggtg aaggaagtaa gaagtttgtc gcggttaaaa
    18601 atgctaactt ctttatcaac aagggagaaa ctttctcgct tgtaggtgag tccggtagtg
    18661 ggaaaacaac tattggtcgt gctatcatcg gtctaaatga tacaagtaat ggagatatca
    18721 tttttgatgg tcaaaagatt aatggtaaga aatcgcgtga acaagctgcg gaattgattc
    18781 gtcgaatcca gatgattttc caagaccctg ccgcaagttt gaatgaacgt gcgactgttg
    18841 attatattat ttctgaaggt ctttacaatc accgtttatt taaggatgaa gaagaacgta
    18901 aagagaaagt tcaaaatatt atccgtgaag taggtcttct tgctgagcac ttgactcgtt
    18961 accctcatga attctcaggc ggtcaacgtc aacgtatcgg tattgcccgt gccttggtca
    19021 tgcaaccaga ctttgttatt gcagatgagc caatttcagc cttggacgtt tctgtacgtg
    19081 cccaagtctt gaacttgctc aaaaaattcc aaaaagagct cggcctgacc tatctcttca
    19141 tcgcccatga cttgtcggtt gttcgcttta tttcagatcg tatcgcagtt atttacaagg
    19201 gtgttattgt agaggttgca gaaacagaag aattgtttaa caatccaatt cacccatata
    19261 ctcaagcctt gctttcagcg gtaccaatcc cagatccaat cttggaacgt aagaaggtct
    19321 tgaaggttta cgatccaagt caacacgact atgagactga taagccatct atggtagaaa
    19381 tccgtccagg tcactatgtt tgggcgaacc aagccgaatt ggcacgttat caaaaaggac
    19441 taaactagta atggttttat aatttccatg tcaattttta tggaaattag gctctttgtc
    19501 aactgtagtg ggttgaagtc agctaagctc gagaaaggac aaatttcgtc ctttcttttt
    19561 tgatgttcaa agcgataaaa atccgttttt tgaagttttc aaagtttcga aaaccaaagg
    19621 cattgcgctt gataagtttg atgagattat tggtcgcttc cagtttggca ttagaatagt
    19681 gtagttgaag ggcgttgata atcttttctt tatctttgag gaaggtttta aagacagtct
    19741 gaaaaatagg atgaacctgc ttaagattgt cctcgataag ttcgaaaaat ttctctggtt
    19801 ccttattctg gaagtgaaaa agcaagagct gatagagctg atagtggtgt ttcaagtctt
    19861 ccgaataact caaaagcttg tttaaaatct ctttattggt taagtgcata cgaaaagtag
    19921 gacgataaaa acgtttatca ctcagtttac ggctatcctg ttgtatgagc ttccagtagt
    19981 gcttgatagc cttgtattca tgggattttc gatgaaactg attcatgatt tggacacgca
    20041 cacgactcat agcacggcta agatgttgta caatgtgaaa gcgatcaaga acgattttag
    20101 cattcgggag tgaaacagtc taggagactg tttcagcctg agcctagaaa tttgaaagcg
    20161 aagctgttta gccaagtcat agtaagggct aaacatatcc atagtaatga ttttgacgcg
    20221 acatcggacg gctctatcat atttaagaaa gtgatctcgg atgacagctt gtgttctacc
    20281 ttcaagaaca gtgatgatat cgagcttttc aaaattttgc gcaataaagc tcatctccat
    20341 ctcccgattg aaacagtcac tccccggact gtttcaacgt cccaagacat aatcttaggc
    20401 agatttctaa aattacactc aaagtgaaag tcattgagct tgcgaatgac agttgaagtt
    20461 gaaatggcca gctgatgggc aatatcagtc atagaaatct tttcaattaa cttttgagca
    20521 atcttttggt tgatgatacg agggatttgg tgattcttct tgacgataga agtttcagcg
    20581 accatcattt ttgaacagtg atagcactta aagcgacgct ttctaaggag aattctagta
    20641 ggcataccag ttgtctcaag gtaaggaatt ttatagggtc tttaatgtct agtaattttg
    20701 tgataaaatg taattgttct atatgattct ttctaatgag ttgttttgtc gcttttcatt
    20761 ataggtcata tgggactttt tttctacaca aaaataggct ccataatatc tataggggat
    20821 ttacccacta caaatattat agagccgaaa aaggctgaga tctggtgtct cagtctttta
    20881 tttcttgcgt tttgcaaaat ctacaaatct aaatttttct aacttatggc gactttcggt
    20941 aaactgaaat tggcgtccgt cttggagata gacttttgat ttaatcgaaa cgacataagg
    21001 gtctttgcca atgtccatga gaatcttgtc tcggtcgctt gagtggtcaa tggtgatttc
    21061 cttctgagca taatcaataa ggagtttgag gccattttct atataagaat agatagactg
    21121 ctcagcaatt tggcgagtga gatttggaat tagttccata tccagatagt ctgtatccag
    21181 aacggatacc agatcatcca ccacacgctg gcggaccacc ttccaaacca tccgaaactc
    21241 tgggaaaccg gttatcagcg aggatttttt atcaataata atcttgtcca gactgaccac
    21301 gttggtttta gagcgcagtc caagttcttt aactagttct tggtagctgg ttaggttgga
    21361 tacagggaaa ttgacggttt cttctttgac gacttgagaa ccttgccctc ttatcttttt
    21421 gatcaatcct tcctcttgga ggagagacag ggcctttcgg acggtatcac gactcacttg
    21481 atattgctcc ataaggtcgt gctcgctagg aaggaaatct ccgacagcgt aagtctcgtt
    21541 ttgaatggtt tcttggattt gcttaaataa ttgttggtat ttcttcattt tgcctgctta
    21601 aataggtttt tagtctagtt taatgaacac ctgtatctat tttaccatac aagtgctaga
    21661 aaatttacaa aaaaacaccc tttttatacc aaacaagttg tagacaagtt tggtatcgtt
    21721 tacaatatac ttatcaaatc aaacttgctt tgacaagtat aaggagaatc aaatgggaaa
    21781 atttgaacaa gaagccaaag atctgcttca ggcaattgga ggcaaagaaa atgtgactgc
    21841 cgtaactcac tgtgcgacac ggatgcgctt tgttttaaga gatgataaga aggctaatgt
    21901 taaagctatc gagtcaattc cagctgttaa aggaactttt acaaatgcag gtcaatttca
    21961 ggtaatcatt ggaaatgacg tgcccatctt ttataatgat tttacagccg tttcaggtat
    22021 tgagggtgtt tccaaagaag caaccaagtc tgcagctaag agtaatcaaa acgtggttca
    22081 aggtgttatg accactctgg cggaaatctt tactccgatt attccagcct tgattgttgg
    22141 tggattgatc ctcggtttcc gtaatgtctt ggaaggtgtc cattggtcga tgttggatgg
    22201 caagaccatc acagaatcct ctcagttttg ggcaggggtt aatcacttcc tctggttgcc
    22261 tggtgaagct atcttccagt tcttaccagt agggattact tggtctgttt ctcgtaagat
    22321 gggaaccagc caaattttgg ggattgttct cggaatctgt ttggtatcgc ctcagttgct
    22381 caatgcctat gcggttgctt caacgccagc agctgatatc gcggcaaact gggtttggaa
    22441 ttttggttat tttactgtta atcgtattgg ttaccaagcc caagttatcc cagccttgct
    22501 tgcaggtttg agtctgtctt atcttgaaat tttctggcgc aaacatatcc cagaagtcat
    22561 ttccatgatt tttgtacctt tcttgtcatt gattccagcc ttgattttgg ctcatactgt
    22621 tttgggacca atcggttgga caattggaca aggactttca tcagttgtct tggcaggttt
    22681 aactggtcca gttaaatggc tcttcggtgc aatttttggt gccctctacg ctccatttgt
    22741 catcacaggt ctgcaccata tgaccaatgc cattgataca caattgattg cggatgctgg
    22801 tggcactgcc ctatggccaa tgattgctct ttctaatatt gctcaaggct cagccgtgtt
    22861 tgcctattat ttcatgcatc gccatgatga gcgtgaggct caggtttcac ttcctgcaac
    22921 catttcagcc tatctcggtg ttacagaacc agctcttttt ggggttaacg taaaatatat
    22981 ttatccattt gttgctggga tgactggttc agcccttgca ggcatgttat ccgttacttt
    23041 taatgtaact gcggcttcta ttggtatcgg tggtttgcca ggtattctct ctattcaacc
    23101 tcaatacatg ctgccatttg caggaactat gctagttgcg attgttgttc caatgctctt
    23161 gactttcttc ttccgcaagg ctggtctctt tacaaaaaca gagggcgata cgaacttgca
    23221 ggcagaattc gttgctcaag aagaagcaga atttgtgagc catgaactag tagaacttac
    23281 ttcggtagaa attatcagcc cactaactgg ccaagtgaaa gaattgagtc aagcgacgga
    23341 tcctgttttt gcatcaggtg tcatggggca aggtctggtc attgaaccaa gccaaggtga
    23401 gttgatctct ccagttaatg gaacagtgac ggttcttttc cctaccaagc atgccatcgg
    23461 cattgtctct gacgagggag ttgaattgct catccacatc ggtatggata cagtaggtct
    23521 tgatggcaaa ggttttgaaa gtcttgtagt ccaaggagat cacgttacag ttggtcagca
    23581 actgattcgt tttgatatgg atgtcattaa ggctgcaggt ctggtgacag aaactcctgt
    23641 tatcatcacc aaccaagatg cttatacagc gactattact ggaacttatc cgacaacgat
    23701 ccaagctgga gcatctctca tggtcgctac acgaatctaa tactcttcga aaatctcttc
    23761 aaaccacgtc agctctatct gcaacctcaa aatagtgttt tgagcaacct gtggctagct
    23821 tcctagtttg ttctttgatt ttcattgagt ataattgaaa tagcacaaat agggacggaa
    23881 tactgcttcc tcccttttat cagaaaagga gaaacagatg acacttgata aaggaaaagt
    23941 agtctatcaa atctatccaa aatcttacaa agacaccact gaaaacggta ttggggattt
    24001 ccgtgggatt atcgaaaaaa ttccctattt agccaaacta ggtgtggata tggtctggct
    24061 caatccattc tatccaagcc ctcaacggga taatggttac gatatttcag attatatggc
    24121 agtggatcct ctttttggtg atatggctga ttttgaggaa atggtgtgtg tcggtaaaga
    24181 gcacaagatt gactttatgc tggacatggt acttaatcat tgttcgacag agcatgaatg
    24241 gtttcagaaa gccctagctg gtgataagta ttatcaagac tttttcttca tccaagacca
    24301 accaacagat tggcagtcta agtttggtgg ttctgcatgg gcgcctttcg gggatactgg
    24361 gaaatactat cttcacctat ttgatgagac ccaggctgac cttaactggc gcaatcccaa
    24421 tgtccgtaag gagcttttca aggttgttaa tttctggcgc gacaagggtg tcaaaggttt
    24481 tcgatttgat gtgatcaatt tgatcggtaa ggacgaggtt tcagtggact gtcctgaaaa
    24541 tgaagggaag ccagcttata cagacaaacc tatcgttcat aactatttgc gtatgatgaa
    24601 ccaagccact tttggatctg acgatagctt tatgacagtt ggggaaatgt cttctaccac
    24661 catggaaaac tgtgtccttt attcgtcgcc tgatcgtcag gaattatcca tgacctttaa
    24721 ttttcatcac ctcaaggtgg actataagga tggacaaaag tggaccttgg ctccctttga
    24781 ttttgaagag ttgaagagtc tttaccatag ttggggtaag gaaatgagtg ataaagacgg
    24841 ttggagtgcc ctcttttgga acaatcacga tcaaccacgt gccttgaacc gttttgtcga
    24901 tattcaaaac ttccgcaagg aaggagccac catgctagca gccagcattc acctgtcacg
    24961 tgggacacct tatatctaca tgggtgagga aatcgggatg attgacccag actatgattc
    25021 tatggctgat tatgtggatg tcgaatcgct taatgcctat cagatgctct tagaagaagg
    25081 aaagagtcag caagaagcct tccagattat tcaagctaag tcgcgtgata attcacgcat
    25141 tcccatgcag tgggatgctt cggaaaatgc aggattttca acaggcactc catggttgaa
    25201 agctggtaaa tcctataaag atatcaatgt agaaaatgaa atcaaaggtc cgattttcac
    25261 cttctatcaa gacttgattc gactgcgtaa ggaaatgcca atcatttcag aaggaaatta
    25321 caaaccagcc tttgaagata gcaaacaagt ctacgctttt gaacgccagt ttgaggatca
    25381 aaagttacta gtgctcaata acttttatgc taaagaagtg gaaatcgact tgccagcagt
    25441 ctaccaaaat ggacagattt tgatatcaaa ctatgaagat gcagaagtgt ctgagaaaat
    25501 tctactcaaa ccctatcaaa cactggctat ttatgtaaat taatactctt cgaaaatctc
    25561 ttcaaaccgt gtcagtttta tctgcaacct caaagcggtg ctttgagcaa cctgtggcta
    25621 gcttcctagt ttgctctttg attttcattg agtataaact tagcaaggag acctcttttt
    25681 cagaaaagca gggttctttt tgctgtctat tccatccttg aaagtgtaga acaaactggt
    25741 cagggtggct agtttgtggt ataatgaaag ggattcaaaa agaaacagga gaaaaatgat
    25801 ggatttactt ttagcaattg tattgattgt gctagctttt ctaggaggag ctcttggagg
    25861 aatgtacttg gttcgtaagc aaatcgaaaa agaatttgct gacaacccac gtttgaatgc
    25921 tgaagcagtt cgtactcttt tgagtgcaaa tggtcaaaaa ccaagcgaag ctaaggtaca
    25981 acaagtttac caccaaatca tccgccaaca aaaggcagcc cttgctaaca ataaaaagaa
    26041 aaaataaata aggaaaagtc tgggatgaaa gttccagact tctcactatg ttggttatgg
    26101 tttagggatg agactttttc cttgcattct attgatattt gattatgatt aagagagagg
    26161 tagttgacat tctatcaagc tgtcggtcag ttcgttcagt acaaggaatc ataatgaacg
    26221 atcaatcaga aaaaagacta gaaagaagac tgtatggata atcgaccaat tggttttttg
    26281 gattcgggtg tcgggggctt gaccgttgtg cgcgagctca tgcgccagct tccccatgaa
    26341 gaaatcgtct atattggaga ttcggcgcgg gcgccctatg gcccccgtcc tgctgagcaa
    26401 attcgtgaat atacttggca gctggtcaac tttctcttga ccaaggatgt caaaatgatt
    26461 gtcattgctt gtaacactgc gactgcggtc gtctgggaag aaatcaaggc tcaactagat
    26521 attcctgtct tgggtgtaat tttgccagga gcttcggcag ccatcaagtc cagtcaaggt
    26581 gggaaaatcg gagtgattgg aacgcccatg acggtacaat cagacatata ccgtcagaaa
    26641 atccatgatc tggatcccga cttacaggtg gagagcttgg cctgtcccaa gtttgctccc
    26701 ttggttgagt caggtgccct gtcaaccagt gttaccaaga aggtggtcta tgaaaccctg
    26761 cgtcccttgg ttggaaaggt ggatagcctg attttgggct gtactcatta tccactcctt
    26821 cgccctatta tccaaaatgt gatggggcca aaggttcagc tcatcgatag tggggcagag
    26881 tgcgtacggg atatctcagt cttactcaat tattttgaaa tcaatcgtgg tcgcgatgct
    26941 ggaccactcc atcaccgttt ttacacaaca gccagtagcc aaagttttgc acaaattggt
    27001 gaagaatggc tggaaaaaga gattcatgtg gagcatgtag aattatgaca aataaaattt
    27061 atgaatataa ggatgaccag gactggtatg ttgggtctta tagtattttt ggtggcgtta
    27121 acagtttgag cgactatcag acagattttc ctctgtttga attctccaaa atatttggag
    27181 atgaagagta tggtttcccg ctttcagtta ctgttttacg ctatggttct acctaccgtt
    27241 tgttctcctt tgtggtagat atgcttaatc aagaaatggg acgaaacttg gaagttattc
    27301 aacgtcatgg ggccctgctc ttggttgaaa atgggcaact cttgtatgta gaattgccta
    27361 aagaaggggt caatgttcat gatttctttg agacaagcaa ggtcagagaa accttgttga
    27421 ttgcgactcg taacgaaggc aaaaccaagg aattccgagc tatctttgat aagttaggct
    27481 acgatgtgga aaatcttaat gactaccctg acctgcctga agtagcagaa acaggtatga
    27541 cctttgaaga aaatgcccgc cttaaggcag aaaccatttc tcaattaacg ggcaagatgg
    27601 ttttggcaga tgattctggt ctcaaagtcg atgtccttgg tggcttacca ggcgtctggt
    27661 cagctcgttt cgcaggtgtg ggagcaactg accgtgaaaa taatgccaaa ctcttgcacg
    27721 aattggccat ggtctttgaa ctcaaggacc gctcggctca gttccacaca accctagtcg
    27781 tagccagccc aaataaggaa agtttagttg ttgaagcaga ctggtcaggt tatattaact
    27841 ttgaacctaa gggtgaaaat ggctttggct atgatcccct cttccttgta ggagagacag
    27901 gtgagtcatc agctgaatta accctggaag aaaaaaatag tcaatctcac cgtgccttag
    27961 ccgttaagaa acttttggag gtatttccat catggcaaag caaaccatca ttgtaatgag
    28021 cgattcccat ggcgatagct tgattgtgga agaagtccgt gatcgctatg tgggcaaagt
    28081 cgatgccgtt tttcataacg gcgattctga actacgtccg gattctccac tttgggaggg
    28141 catccgcgtt gttaaaggga acatggactt ctacgccggc tacccagaac gtctggtgac
    28201 tgagcttggt tcgaccaaga ttatccaaac tcatggtcac ttgtttgaca tcaatttcaa
    28261 ctttcaaaag ttggactact gggctcagga ggaagaggcc gctatctgcc tctatggtca
    28321 cttgcatgtg ccaagtgctt ggttggaagg caagatcctc tttctaaatc caggctctat
    28381 cagtcaacca cgaggtacca tcagagaatg tctctatgct cgtgtggaga ttgatgatag
    28441 ttacttcaaa gtggactttt tgacacgaga tcacgaggtg tatccaggtt tgtccaagga
    28501 gtttagccga tgattgccaa ggagtttgag actttcttgt tggggcagga ggaaactttt
    28561 ttgacccctg ctaaaaatct agctgtgttg attgatactc acaatgcgga tcatgcgacc
    28621 ctcttgctca gtcagatgac ctatacccgt gttcccgttg tgacagatga aaaacagttt
    28681 gttgggacga ttggactcag agatattatg gcttatcaga tggagcatga cttgagccaa
    28741 gaaatcatgg cggatacgga tatcgttcat atgacaaaaa cggacgtagc ggttgttttg
    28801 cctgatttca ccattacgga ggtcttgcac aagctagtag atgagtcctt cttaccggtt
    28861 gtggatgcag agggtatttt ccaagggatt attacgcgca agtccatcct caaggccgtt
    28921 aatgccctct tgcatgactt tagtaaggaa tatgagattc gatgccaatg agagacagga
    28981 tttcagcctt tttagaggaa aagcagggct tgtctgtcaa ttccaagcag tcctataagt
    29041 atgatttgga gcaattttta gacatggtag gtgagcggat ttctgagacc agtctcaaga
    29101 tttaccaagc ccagctagcc aatctaaaaa tcagcgccca gaagcgaaag atttcggcct
    29161 gtaaccaatt tctatacttt ctctatcaaa aaggagaggt ggacagcttt taccgcttgg
    29221 aattagccaa acaagctgaa aagaagacgg aaaagccaga gattctatac ctagactctt
    29281 tttggcagga aagcgaccat ccagagggcc gcttgctagc gctcttaatc ctagaaatgg
    29341 ggctcttgcc cagtgagatt ttagccatca aggttgcgga catcaatctg gattttcagg
    29401 tgttgcgaat cagcaaggct tcccaacgga ggattgtcac cattcccacg gccttgcttt
    29461 cagaattgga acccttgatg gggcagacct atctttttga aagaggaggg aaaccctatt
    29521 ctcgtcagtg ggcctttcgt cagttagaat cttttgtcaa ggagaaaggt tttccatcct
    29581 tatcagctca agtcttacgt gaacagttta ttctaagaca aatagaaaac aaggtcgatt
    29641 tgtacgaaat tgcaaaaaaa ttaggattaa aaacagtcct gaccttagaa aaatatagat
    29701 aatggatatt aaattaaaag attttgaagg acccctggac ttactcttgc atctggtttc
    29761 taagtaccag atggatatct acgatgtgcc cattacggaa gtcatcgaac agtatctagc
    29821 ctatgtttca accctgcagg ccatgcgtct ggaagtgacg ggtgagtaca tggtcatggc
    29881 tagtcagctc atgctgatta agagtcgtaa actccttccg aaggtagcag aagtgacaga
    29941 cttgggggat gacctggagc aggacctcct ctctcaaatc gaagaatatc gcaagttcaa
    30001 gctcttgggt gagcacttgg aagccaagca ccaagaacgg gcccagtatt attccaaagc
    30061 gccgacagag ttgatttacg aagatgcgga gcttgtgcat gacaagacga ccattgacct
    30121 ctttttggct ttttcaaata tactagccaa gaaaaaagag gagtttgcac aaaatcacac
    30181 gacgatcttg cgggatgagt ataagattga ggacatgatg attatcgtga aagagtcctt
    30241 gattggacga gatcaattgc gcttgcagga tttgttcaag gaagcccaga atgtccaaga
    30301 ggtcatcacc ctctttttgg caaccctaga gttaatcaaa acccaggagc tgatcctcgt
    30361 gcaagaggag agttttggag atatctatct catggaaaag aaggaagaaa gtcaagtgcc
    30421 tcaaagctag acttgataga gaggaaagat gagtacttta gcaaaaatag aagcgctctt
    30481 gtttgtagcg ggtgaagatg ggattcgggt ccgccagtta gctgaactcc tctctctgcc
    30541 accgacaggc atccagcaaa gtttaggaaa attagcccag aagtatgaaa aggacccaga
    30601 ttccagtttg gctttgattg agacaagtgg tgcttataga ttggtgacca agcctcaatt
    30661 tgcagagatt ttgaaggaat actctaaggc gcctatcaac cagagcttgt ctcgggctgc
    30721 ccttgagacc ttgtccatta ttgcctacaa acagccgatt acgcggatag aaattgatgc
    30781 catccgtggg gttaactcga gtggagcctt ggcaaagttg caggcttttg acctgataaa
    30841 ggaagacggg aaaaaggaag tattggggcg ccccaacctc tatgtgacta cggattattt
    30901 cctagattac atggggataa accatttaga agaattacca gtgattgatg agcttgagat
    30961 tcaagcccaa gaaagccaat tatttggtga aaggatagaa gaagatgaga atcaataagt
    31021 atattgctca cgcaggtgtg gccagtagga gaaaagcaga agagctgatt aagcaaggct
    31081 tggtgacggt taacggccaa gtggtgcgtg aactggcaac cactatcaag tcaggcgaca
    31141 aggtcgaagt tgaaggtcaa cctatctaca acgaagaaaa ggtctactat ctgcttaaca
    31201 aaccacgtgg tgtgatttcc agtgtgacag atgataaggg tcgcaagacg gttgtcgacc
    31261 tcttgcccaa tgtcaaagaa cgtatttacc ctgtgggtcg tttggactgg gatacatcag
    31321 gtgtcttgat tttgaccaat gatggggact ttacagacga gatgattcac cctcgtaatg
    31381 agattgacaa ggtttatgtc gcgcgtgtta aaggtgtggc caataaggac aatcttcgcc
    31441 ccttgacccg tggtcttgag attgatggta agaaaaccaa gccagctgtt tatgaaattc
    31501 tcaaagtgga cccagtcaaa aatcgctctg tggtgcagtt gaccatccat gaagggcgta
    31561 accatcaagt taaaaagatg tttgaagctg ttggtctcca agtagataag ttgtctcgga
    31621 ctcgtttcgg acacctagac ttgacaggac tccgtccagg agaatctcgt cgtcttaata
    31681 aaaaagaaat cagccaacta cacaccatgg ctgtaactaa gaaataatga aacgaatttt
    31741 aatagcgcct gtgcgctttt accaacgttt tatctcacca gtctttccac cctcttgtcg
    31801 ctttgatctg acttgttcca actacatgat tcaggctatt gaaaaacatg gttttaaggg
    31861 ggtattgatg ggcttggctc ggattttacg ttgtcatccc tggtcgaaaa caggtaagga
    31921 ccccgttcca gaccgctttt cccttaaacg aaatcaagaa ggggaatgag gttgggtaaa
    31981 tagatttcaa aatgataaaa acgcatccta tcaagtttga gtgaacttga taggatgcgt
    32041 tttagaatgt caaaatttta tactcttcga aaatctcttc aaaccgcgtc agctttcatc
    32101 tgcaacctca aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat
    32161 tttcattgag tattaaattg agtttgaagt ggcttatttc aaagcttttt gtatgtcttc
    32221 aatcatgagt tttgttgatt caagtccgcc tccgcttaga taccagaggt ctggtgttag
    32281 ttggataatc ttaccatttt tagcagcagg tgtttcagcg ataagggcat tttctaggac
    32341 accgtcgttg ctagagttgt ccccaccgat ggcaagggta cggttgatga caaagaggat
    32401 gtcagggttg atttctttga cactttcaaa gctgatttct tgtccgtggc gtgagtcttc
    32461 aaattttgta tcagttggtt tgaatttcaa ggtttggtac aagaaagaga aacgagattt
    32521 ggcaccaaag gctgccattt ttccttcatt aaggaggatc gcaagggctt ttttgtcaga
    32581 gctttcattt ttagtagcga cttcttggat gctcttgtct agcttggcca attcttcctt
    32641 ggctttctgt gtaccagttt cgccgaaggc acttgctaag gattcgatat tagccttggt
    32701 agaagtccag tagtcgtcct tgcttgcttg gaagagaacg gttggggcga tttctttgaa
    32761 tttgtctacg aatttttgtg tacgtggtga agcgataatc aaatcaggct caagggcggc
    32821 gatagcttct aaatcaggtt ctttcataga accaacattt ttgacagttc ccactaggtc
    32881 ttttagataa gtcggaacag tttttgtagg cattccgacg atattttttt caaatcctaa
    32941 agcgcgaata gtatccgcag cgccgaggtc aaaggtcaca atcttttcag gaactttgga
    33001 aagtttgacc tcgtccagtg aacttttaat ggttacctct gttggagcag agctactggt
    33061 ctctgtctga ctagtgcttg agtttgtact acatgcacca agtaggagca agaagctggc
    33121 cactagggca gtgaaataaa gtttaaggga tgttttcata atttctcctt tttaaaatgt
    33181 gataacgatt tagggagtct cttgatctta ttgactaaga gactgaaggt tctctaactt
    33241 gagcttttat gttactagct atagatacag atctttttgt cattgatatc agctagcgtg
    33301 atgggaatct cataaagttg actgagcagg tcagcctgca tgatttgatc ggttcttccc
    33361 ttgctaaaga cctggccgtc cttgaaggcg acaatttcat ctgcatactg actggccatg
    33421 ttgatatcgt ggaggacgat gataatggtc ttgccgagtt cctccaccag tcgtcgaaga
    33481 atctgcatca tgctgacgct ttgcttgata tcgagattgt tgagtggttc gtccagcaag
    33541 ataaagtccg tatcctgggc cagtaccata gcgataaaga cgcgctggag ttgcccccct
    33601 gacaggctat tgatgtagcg gtcttttaag ttggtcagtt ccaaatagtt cagagtttct
    33661 cggatttttt cccagtcttc tgatctaagt cgacctcggc tgtagggaaa acgtccaaaa
    33721 ctgaccagtt cttcaacagt caatttggct tggtaattga ttttctgttt taggatggtt
    33781 agttcttggg ccagttcttg cgaattccag ctatcgattt cacgtccttt gatactgaga
    33841 actccctgat ctttcttggt tagtctgctc atgatggaga ggagagtcga ttttccagca
    33901 ccatttggac caataaaggc tgtcagtttt tgaggactga cttcaagcga aatgccttgc
    33961 aaaatatcct gtttttgaat ggatttgtca atgttttcca gtttcactga cgagacctcc
    34021 tatatagtaa gataaagaat aagaagccac ccacactctc aatgatcata ctgatacgaa
    34081 tttccagtgc aaagactcgt tcaatcaagg cttgccccaa ggttaagcta ataaatccaa
    34141 tcagaatggc cactataaag agtaacttgt gctgatagtc tttgacaatc aggtaggtga
    34201 ggttggccag cataaagccg aagaaggcca taggtcctac caaggcagtg gccgttgagg
    34261 tcaaaagcac gattccccag aggagctctt tctgttcttt ttcaacatcg agtcccaata
    34321 tctgagccgt ttctctttgc aggtgcaaga catctagaac gactgctttt cgaaagaaaa
    34381 agattgtcaa agcgaggatg atcagagaac cgatggctag gatggaagtg ttgagatgtt
    34441 gaaaggaggc aaaaagacta ttttgcagtt tatcgtattc gtttggatcc attaggactt
    34501 gaaggaaggt gctgatattt cgaaagagac ttctgagcgc tagacagatc agcaggatga
    34561 agaccaggtc ttgcttcatc agtgtcttca agtaaccttg taaggcgaga aagaagaggg
    34621 actggacaag aagtaagact aggaattcta agatagggga tttgccaagt tgaagaaact
    34681 tgctttcaaa aaccagtagt agggtttgta gtaggacgta gaaggattca attcccaaaa
    34741 tactaggcgt caggaagcga ttttccgtca gggtttgaaa actaatggtc gaaatcccag
    34801 tcgcgatggc taccaagaga taaacgatga tcttttggga acgcaacttc caagcaaagg
    34861 ctgacaagtg agtgatgggc caaaagtaga gaagacaagc tccgatggca agaataatga
    34921 gaatccagaa gagcttggta tgtttgcttt tagtctgcat cttttcgtcc ccctctccag
    34981 agaagtagga taaagacgag actaccgatg attcctagca agagactgac agacaactca
    35041 tagggcctaa tcagaactcg ggataggata tcgcaagcca gaactagatt ggcaccaacc
    35101 agtgcgacca tgagtttggt ttgacttaga ttatctccat agcacttgcg aacaagattg
    35161 ggaacgataa ctccgagaaa tggtaggcca cccacggtaa tcatggtgac gcttgtcgtt
    35221 agcgccacca gaaagagggc cagtttttca agtagggagt aggaaatccc caaactctcg
    35281 ctggtttctt tccctagatt catgatggtg aaggtttggg ataatttcca aacggttatc
    35341 aggatgatga ggcctaagaa gagccactca tactgatggg tctgaatcat ggagaaggag
    35401 ccctgggtcc aggcagtcat actctgaacc agattgaaac gataggcgat aacttctgtg
    35461 actgagccga taatcccgct atagatgatc ccaatcagag gcaacatcca cctttccttt
    35521 acagtaaaaa tggtcataaa ggctaggaag aagagggtga atacgatgga tgaaacaaaa
    35581 gcgaagagca tcttttgggt cagactagcc gatggaaaga caaaaaggct cagcaccatt
    35641 cccagtttgg cggcttcagt cgttccaact gtactcggtg cagcaaactg attttgggta
    35701 atagtctgca tgagaaggcc tgccatactc atactagagg cagtcaggag aatactgata
    35761 gttcttggga gacgggactc ttgaaagagg agccaggtct gctggtcgaa atcaaatagc
    35821 tttccccatg aaaaatcact ggtcccaatg ctaatagaga gaaagactag gagtagaagt
    35881 aagccaatta aataatgaga aagtttcata ccccgtcctt tcatgtagat ttggtatcga
    35941 aagatatctg cggatataaa tgtaacatta tttttctaat ctgtcaataa aatttctgac
    36001 aatttaataa atacaacaag gagagagcaa caagactttc tcctttgtta tcctattcta
    36061 aaatgttttt accttaatct gataaaataa tatcttcgag ggagtagcta gccgtccaat
    36121 caagatattg tttagctttt gaagcatctg ctaggacact ggctgggtca ctagcacgtc
    36181 gagcaacaat ctcgtgtggg attttttaat ttagtaattc ttcagcagtt ttaaagattt
    36241 ctttgatagt atagcctttt ttagttccta agttaaagat ttgagaagaa ctgtcttctt
    36301 gaaataggta gttcattcct ttaacatgag cctatgcaag gtccaagaca taaatgtaat
    36361 ctcgaataca tgaaccgtca tgtgtatcgt agtcatctcc aaatgttttt aagctatcat
    36421 tttgtcccaa tgcggtcttg ttgatatttg gaatgatgtg agttggattt ttcacacgca
    36481 gaccgtttga agcatccatt tcagccccag caacattaaa gtaacggaaa ataacatatt
    36541 tccagtcgta gcgattggcc atccagtaaa tcattcgttc gcccatcagt tttgtctctg
    36601 cataagggtt gacagggtcg agcagggtat cttcagtcac cggcttgtca atacagttat
    36661 ttccatagag agaagcagtc gaagagaaca tgattttttg aatgccaact tcagataaga
    36721 ctttgagaac ttggttcata ccagcaacgt tggcagtgaa gtatttactt ggattttcaa
    36781 tactttcgct cacaacaatt tcacctgcac aatggaggac agcatcaatc tgattttctt
    36841 ctaagtaaac cttcaaggcg ctagcatcat aaacatccag ttctttaaag ctagcacgac
    36901 tatccacagc cgctcgattt cctgtggaga gattatcgag gacatgcacc ttatagccag
    36961 catttaaaag agctttaacg gtatgggagc caatgtagct agtcccacct gtaacaagaa
    37021 ttgttttggt catgattttt tccttttcta gttttgtact atagtaaatt gaaataagat
    37081 atgaacaact ctattaggaa agtcaaatta atttctagaa atgttttagc agctacagcg
    37141 tactattcca aactcaacca actatatatt taaagaaaga agaaagggaa gccaactatt
    37201 ttctgtaaat ttcatgaaaa atccaaacaa ctttgatggt gtattgtttt ttgtttggaa
    37261 ataaatattc gagttaattt tggaatgttt actagatttt gcactacatg caagagaata
    37321 ctatcctttt ctaagataga gcaggactaa gatactaact tagaaatcca ccttcccact
    37381 atccttctcc tataaaaagt ggtaaaatgg atgaaagaaa gaaggaactg aaatgacaac
    37441 attattttca aaaattaaag aagtaacaga acttgctgca gtctcaggtc atgaagcgcc
    37501 tgtccgtgct tatcttcgtg aaaagttgac accgcatgtg gatgaagtgg tgacagatgg
    37561 cttgggtggt atttttggta tcaaacattc agaagctgtg gatgcaccgc gcgtcttggt
    37621 cgcttctcat atggacgaag ttggttttat ggtcagcgaa atcaagccag atggtacctt
    37681 ccgtgtcgta gaaatcggtg gctggaaccc catggtggtt agcagccaac gtttcaaact
    37741 cttgactcgt gatggtcatg aaattcctgt gatttcaggt tctgttcctc cgcatttgac
    37801 tcgtggaaag gggggaccaa ccatgccagc cattgccgat atcgtttttg atggtggttt
    37861 tgcggacaag gctgaggcag aaagttttgg catccgtcct ggtgatacca tcgtaccaga
    37921 tagttctgca attttgacag ccaatgaaaa aaatatcatc tcaaaagctt gggataaccg
    37981 ctacggtgtc ctcatggtaa gcgagctagc tgaagcttta tcaggtcaaa aactcggcaa
    38041 tgaactctat ctgggttcta acgtccaaga agaagttggt ctgcgtggcg ctcatacctc
    38101 tacaaccaag tttgacccag aagtcttcct cgcagttgat tgctcaccag caggtgatgt
    38161 ctacggtggt caaggcaaga ttggagatgg aaccttgatt cgtttctatg atccaggtca
    38221 cttgcttctt ccagggatga aggatttcct tttgacaacg gctgaagaag ctggtatcaa
    38281 gtaccaatac tactgtggta aaggcggaac agatgcaggt gcagctcatc tgaaaaatgg
    38341 tggtgtccca tcaacaacta tcggtgtctg cgctcgttat atccattctc accaaaccct
    38401 ctatgcaatg gatgacttcc tagaagcgca agctttctta caagccttgg tgaagaaatt
    38461 ggatcgttca acggttgatt tgattaaaca ttattaaacc ataagggagg tggtagggat
    38521 ggtagaacca aacctagaaa gccttataaa agatctttac aatcatgctc gacatgattt
    38581 gagtgaagat ttagttgctg ctctcctaga gactactaaa aaactgccta ctacaaatga
    38641 gcaattgcag gcagttcgtc tctcaggcct ggtcaatcgt gaattgctcc taaatcccaa
    38701 acatccagca cctgagttgc tcaacttggc tcgctttatc aaaagagaag aagccaagta
    38761 cagaggaact gcgacttctg cgcttatgta tgaggaactc tttaaaatgc tttgattcca
    38821 ttgtggatca aagcattttt tgtaagatct atctcgtgag cgttcaaata gtagtaacaa
    38881 ggaatcatga gaagtcagcc gagcccatag tagtgaagaa acttctgtaa tggaagtgga
    38941 gcgaagtgag gaatcgtcaa acggtagggg ataggctgtt tgaagggtgc tacgaaaaag
    39001 atagggtaag gtgaaattat agcgggatga aataagatat gaacaaattg attaggaaaa
    39061 tcaaatgaat ttatagaaat cttttagcag tcatggcgtc ctattctagt ttcaaaatgc
    39121 tataaatacg aacaaattga ttgggaaatt caaatcgctt tctatcaatg ttttagaaga
    39181 tacagtgctc tagtctaact tcaatatact ataaaagaac agatggaagc aattatgatt
    39241 tacttctatc tttaaaataa atggtctgat aggaaaaatg tgtcccctca taaaatatag
    39301 gatagtacga atggaaggct tttgtagact ggaggacgaa ggagtttaga aactctcgtt
    39361 aaaagttagt tgagccggag tatggtggtg tggataatta tcaaaaatta tttctaaatc
    39421 gaaatagttg acagttagaa cgatgagtgt tacaattatt ttattcattt cgatatcgaa
    39481 ataaattgga ggtgttatga aagatggtca tttgctagcc catcatattc gtttgttgaa
    39541 tgggcggatt tttcaaaagt tactgagtca agatcctgag gctctttata ggggtgaaca
    39601 gggcaagatt ttagcggttt tatggaatag tgaaactggc tgcgcaactg cgacagatat
    39661 cgcgcttgcg actggacttg cgaataatac gctgacgact atgataaaaa agctagagga
    39721 acaaaagctt gtaattgtta gtccgtgtgg aaaagacaag cgtaagaagt atttagtttt
    39781 aacggagtta ggcaagtccc agaaagaagt ggggcatcgt gtcagtcaga aattggatac
    39841 tatcttttac aaaggatttt cagaggaaga aattcaccaa tttgaaggtt ttcaagaaag
    39901 aattttggcg aatctgaaag agaagggaaa tgaggtttag aatatgtcac aacaagtgaa
    39961 aaatgctcac aacctataca tccatgccat tcaagacgga cgagttgctg aggctcaagc
    40021 ccagtctgta ggggatacct atatccaaca ttcgacaggt gttcctgacg ggaaagaagg
    40081 atttgcagcc ttctttgcag atttctttga gcgtcatcca gagcgtcaga ttaagattgt
    40141 ccgcaccatt gaggatggca atctggtctt tgttcatgtt catcaatatc tgaatggtgg
    40201 agaagctcaa tgggtgacga cggatacttt ccgtgcggat gagaatggtc gtattgttga
    40261 gcattgggat gtcattgact actatcgaac acctgaaaat gaccaactag atcaaatttt
    40321 tggagatttt gaaatcaagg atttggacaa gaaagcagaa aataaaaagc tggttcgccg
    40381 tttcttgaca gaaattttcc aaaatgggga gctagagcag tggagtgatt atgtggcaga
    40441 cgatttgatt cagcataatc atgatattgg acaaggaagt gctgcttata aaaactatgt
    40501 ggctgaatat agtgtcactt ttgactttgt tttccaactc ttaggacaag gaaactatgt
    40561 ggttagctat ggtcagactc agattgatgg cgttgcttat gcccagtacg atatcttccg
    40621 tttagagaac gggaaaattg tggagcattg ggataataag gaagtcatgc ctaaggtaga
    40681 agacttgacc aatcgaggga agttttaaat tgaggacaaa gaatgattga atacaaaaat
    40741 gtagcactgc gctacacaga aaaggatgtc ttgagagatg tcaacttaca gattgaggat
    40801 ggggaattta tggttttagt agggccttct gggtcaggta agacgaccat gctcaagatg
    40861 attaaccgtc ttttggaacc aactgatgga aatatttata tggatgggaa gcgcatcaaa
    40921 gactatgatg agcgtgaact tcgtctttct actggttatg ttttacaggc tattgctctt
    40981 tttccaaatc taacagttgc ggaaaatatt gctctcattc ctgaaatgaa ggggtggagc
    41041 aaggaagaaa ttacgaagaa aacagaagag cttttggcta aggttggttt accagtagcc
    41101 gagtatgggc atcgcttacc tagtgaatta tctggtggag aacagcaacg ggtcggtatt
    41161 gtccgagcta tgattggtca gcccaagatt ttcctcatgg atgaaccctt ttcggccttg
    41221 gatgctattt cgagaaaaca gttgcaggtt ctgacaaaag aattgcataa agagtttggg
    41281 atgacaacga tttttgtaac ccatgatacg gatgaagcct tgaagttggc ggaccgtatt
    41341 gctgtcttgc aggatggaga aattcgccag gtagcgaatc ccgagacaat tttaaaagcg
    41401 cctgcaacag actttgtagc agacttgttt ggaggtagtg ttcatgacta atttaattgc
    41461 aacttttcag gatcgtttta gtgattggtt gacagctcta tctcaacatt tgcagttgtc
    41521 acttttgacc ttgttactag ctattttgct tgcgattccc ttggctgttt ttcttcgcta
    41581 tcatgagaag ctggccgact gggtcttgca gattgcaggt attttccaga ccatcccgtc
    41641 tctggccttg ttgggactct ttattccctt gatgggaatt gggactttac cggcattgac
    41701 agctctagtg atttatgcga tttttccgat tttacaaaat actattactg ggctgaaggg
    41761 aattgatccg agtcttcaag aggctgggat tgcctttggg atgaccagat gggagcgtct
    41821 caagaagttt gaaattccac ttgccatgcc tgttatcatg tctggaattc ggacggcagc
    41881 ggtcttgatt atcggtacgg ctaccttggc gaccttgatt ggggcagggg gactgggttc
    41941 ctttatcctg ttgggaattg accgtaataa tgccagtttg attttgattg gggccctttc
    42001 ttctgcagtg ctagctattg cctttaactt cctactaaaa gtaatggaaa aagcgaaatt
    42061 gcggacgatt ttctctggtt ttgccttggt ggctttatta ctgggtctgt cttatagtcc
    42121 agcccttttg gctcaaaaag agaaggaaaa cttggttatt gctgggaaaa taggcccaga
    42181 accagaaatt ttggctaata tgtataaatt gctgattgaa gaaaatacca gcatgactgc
    42241 gactgttaaa ccgaattttg ggacgacaag tttcctttat gaagctctga aaaaaggtga
    42301 tattgacatt tatcctgaat ttaccggtac ggtgactgaa agtttgcttc aaccatcacc
    42361 caaggtgagt catgagccag agcaggttta tcaagtggcg cgtgatggta ttgccaagca
    42421 ggatcatcta gcctatctca aacctatgtc ttatcaaaat acctatgctg tagctgttcc
    42481 gaaaaagatt gctcaagaat atggcttgaa gaccatttcg gatttgaaaa aagtggaagg
    42541 gcagctgaag gcaggcttta cgcttgagtt taacgaccgt gaagatggaa ataagggctt
    42601 gcaatcaatg tatggtctca atctcaatgt ggcgactatg cagccagctc ttcgttatca
    42661 ggcaattcag tcaggggata ttcaaatcac ggatgcctat tcgactgatg cggaattgga
    42721 gcgttatgat ttacaggtct tggaagatga caagcaactc ttcccacctt atcaaggggc
    42781 tccactcatg aaagaagctc ttctcaagaa acacccagag ttggaaagag ttcttaatac
    42841 attggctggt aagattacag aaagccagat gagccagctc aactaccaag tcggtgttga
    42901 aggcaagtca gcaaagcaag tagccaagga gtttctccaa gaacaaggtt tgttgaagaa
    42961 atgatgggac agagacttta aacatactga actcaacttc cttaaacgac tagataaaag
    43021 aatgctcaga cagtttcgtc tgggcttttt tgatgtgaga atgactgaat gtcacttaaa
    43081 atggaaagaa agagaaaatt ttcatgattt tctaaaattt tactgtaaag acagttgact
    43141 attcataaaa taggtgtata atattttgtg aactacttaa caaataagag gtttcagctc
    43201 atgtcgacta aggttagaaa tcttgtgtat aggcagttca ttatctaata gagcgttgcg
    43261 tccgaaagtc tatccagaca cggctcttta aaaacaaaag gagaaatgat gcatacttat
    43321 ttgcaaaaga aaattgaaaa tatcaaagca accctaggtg aaatgtcagg tggttaccgt
    43381 cgtatggttg cggctatggc tgatttagga ttttcaggaa ctatgaaggc tatctgggat
    43441 gacctctttg cccatcgtag ttttgcccag tggatttatt tgctggtttt aggaagtttt
    43501 cctctctggc tggagttggt ttacgaacat cgtattgttg actggattgg gatgatttgt
    43561 agcttgacag ggattatctg tgtaatcttt gtatcggaag gtcgagcaag taattatctt
    43621 tttggcttga ttaactctgt tatttacctt attttggccc tacagaaagg cttttatggt
    43681 gaggtgctga cgacacttta cttcacagtc atgcagccaa ttggacttct agtttggatt
    43741 tatcaggcac agtttaagaa ggaaaagcag gagtttgtcg cgcgtaaact ggacggcaag
    43801 ggctggacaa agtatctttc cattagtgtg ctttggtggt tggcctttgg cttcatttat
    43861 cagtctattg gtgccaatcg tccctatcgt gattcaatca cagatgcaac caatggggta
    43921 gggcaaatcc tcatgacagc tgtttaccgt gaacagtgga tattctgggc ggctaccaat
    43981 gttttttcaa tctatctctg gtggggagaa agcctgcaaa ttcaagggaa atatctaatt
    44041 tatctcatta acagtctagt tggttggtat caatggagca aggcagctaa gcagaatact
    44101 gatttaccta actaggaaaa gatgtttgaa agtgctgttt tgagatttcg attaaaacag
    44161 atatagttga taatcaagga tttatagtat gaaaaagagg atcggcgggt cctcttttgt
    44221 tgttgaaaag ataaaaaact cagtaaccta gaaataagac aactgagctt tactctatat
    44281 tcaattttta ggaatgagaa ggtctagata aaattggaca acttcctggt ctgtgaaatc
    44341 ttgacctttt ttgagccacc aggtcaatgt ctcgataaag ttggacacga ccaagtgttg
    44401 gaggtaagaa gtaggcatat tagggtgggc ttcttttaaa ttatcagcta gcacggaata
    44461 gacatggtgt tctagctctt tatggagttg acggaggaag tagtcatttt tggaaaatag
    44521 cagactggtg atatggtctt ggtttttatg aaaatggaga aagaggtggg cgaggtagtc
    44581 ctcggttgaa atggcttgct ctctttcaaa aagatgatgg aagaggtagc ggcagagctg
    44641 gtccagaaga agctccttac tttcatagtg acagtaaaag gtggatcttc ccacatctgc
    44701 gagatcaatg atatcctgaa cagtagtggc ctcgtagccc ttagcattca aaagttgtat
    44761 aaaagcttga tagatggctt ttttggtttt gctgatacgg cggtcaatgt tagtcatatg
    44821 gacacttaag gcaaattgtt cagaactgaa taaagctgac gttttgcttc tatcctttct
    44881 ttgagtttta gtggataatg ataatgaaca aggtgttcat aaacctatta taacaaagga
    44941 atgagaaata tgaaggcaaa atatgctgtt tgggtggctt ttttcttaaa tttgacttat
    45001 gccattgttg agtttattgc aggtggagta tttggttcta gcgctgttct tgctgactct
    45061 gtgcatgact tgggagatgt gattgcaatt ggaatatcag cttttctaga aacaatctcc
    45121 aatcgtgaag aagacaatca gtacaccttg ggctataagc ggtttagcct gctaggagcc
    45181 ttggtaacag ctgtgattct cgtaacaggc tctgttctag tcattttgga aaatgtcacg
    45241 aagattttgc atccgcaacc agtcaatgat gaggggattc tctggttagg aattattgcg
    45301 attactatca atctgttagc gagtctggtg gttggtaagg gaaagacaaa gaatgagtct
    45361 attctgagtc tgcattttct ggaagatacg ctagggtggg tagctgttat cctgatggcg
    45421 attgttcttc gatttacgga ctggtatatc ctagatcctc ttttgtccct tgtcatttct
    45481 ttctttattc tttcaaaagc cctttcacgt ttttggtcta cactcaagat tttcttggat
    45541 gctgtgccag aaggtcttga tatcaagcaa gtaaagagtg gcctggaacg attggacaat
    45601 gtggccagcc ttaatcagct taatctctgg actatggatg ctttggaaaa aaatgccatt
    45661 gtccatgttt gtctaaaaga aatggaacat atggaaactt gtaaagagtc tattcgaatt
    45721 ttcctaaaag attgtggttt tcaaaatatt accattgaaa ttgatgctga cctagaaact
    45781 caccaaaccc ataagcgaaa ggtgtgtgac ttggaacgga gttatgagca tcaacattag
    45841 aaaaaagtga aaaatacttg ggtactatct tatttggaat agagtaattt ctttattatt
    45901 taaatatttc aaaaattggt aagagaagag cattgtataa actccagata tatgattgtt
    45961 aatgataaaa atttttcgat tagatacaaa atgcttgact tggagtcaac tcaaagttat
    46021 ataataagat aagtgagtta gaatagcgtg aattcagtga atgaaatgag aggaggttag
    46081 cgtgtgaata ttaaatctgc cagtgatttg ttgggaattt cagcggatac gattcggtat
    46141 tatgaacggg ttggtcttgt gccaccgatt actcgtactg ctactgggat tcgtgatttt
    46201 caagatcagg atatcgaagc gctggaattt attaagtgtt ttcgttcggc gggtgtctct
    46261 gtagatagtt tagttgacta tatgtcgctc taccaaaagg gagatgaaac gagagaggag
    46321 aggcttggta ttttagaaga ggaaaagcaa aaattagagg agcgcttgtc tcagctacag
    46381 acagctttaa atcgtttaaa tctcaaaatt aaactttata aggaaggaaa attttaaatg
    46441 aaatcagcag tatatacaaa ggcaggtcag gttggacttg ctagcattga acgtccgcaa
    46501 ataatagaag cggatgatgt gattattcgt gtggttcgtg cgtgcgtttg tggttcagat
    46561 ttatggaggt accgtaatcc agaaacgaaa gctggacaca aaaatagtgg acacgaagcg
    46621 attgggattg ttgaagaagc tggggaagcc attacgacgg tgaaaccagg tgattttgtg
    46681 attgtccctt ttacacatgg atgtggtgag tgtgatgcct gtcttgctgg atttgacggt
    46741 tcttgcgaca atcatattgg caataatttg gggggtgatt ttcaggcaga atatattcgc
    46801 ttccactatg caaactgggc gctggttaaa atccctggtc aaccttctga ctatacagaa
    46861 gggatgctca agtccctttt gactcttgca gatgtcatgc cgacaggcta tcatgcggcg
    46921 cgtgttgcaa atgttcaaaa aggggacaag gttgttgtta tcggtgatgg ggctgttggt
    46981 caatgtgctg tcatcgcggc taagatgcgt ggagcatcac aaattatcct tatgagccgt
    47041 catgaagacc gtcaaaagat ggctatggag tcaggtgcga cagctgttgt tgcagaacgt
    47101 ggtcaagaag gaattaccaa ggtgcgtgaa atcctcggtg gaggagcaga tgcagcactt
    47161 gaatgtgttg gtacggaggc tgctatagaa caggcgctag gtgttcttca taatggaggg
    47221 cgtatgggct ttgtaggagt cccacactat aataatcgtg ctcttggttc gacatttatg
    47281 caaaatatct ctgtagcagg tggggcagct tctgctacaa catacgataa gcaattttta
    47341 ctaaaagccg tccttgatgg tgatatcaat ccaggtcgcg tctttacttc aagttataaa
    47401 ctggaagata tcgaccaagc ctataaagat atggatgaac gtaagacaat taagtctatg
    47461 attgtaatcg aataaaaaac gaataggagt tttagaactc tattcgtttt ttatgttatc
    47521 ctattcttga tttagggtac tttctcttaa tgtcagtctg gttcccagca tggtcaggct
    47581 aggaattttg cgaccgtgga ggacttcctt gttaagaata tccatacctg ctcgccccat
    47641 ttcttcagta taaactgtga tgctggagag gggaggataa acctgcttgg tcaggctggt
    47701 gtcgttaaag gaaatgaggc tgacacggtc tggtaggctg attccagctt cttggagcgc
    47761 acgaagggca ccgatggcta aactatcgct ggctgcgaaa aatgctggtg ggagttggtt
    47821 tcccaagctc tgaatggctt ccttcatcaa gtcatagcca gactgggcag taaagtttcc
    47881 ttgaaagacc agttcatcat gatagattcc ttttgcttga ctgtagtttc tgaaattttc
    47941 cagccgctta tcctgaatga tttcttcttg gtcagtcgtt tcttcaaggc ctgttagaat
    48001 cccgatacgg tccattcctt ggctgaggaa ataatcgaca acctgtttca tagcagtgta
    48061 aaaatccgtg ataatacagg tatgtcccag ggaaagtgta tcactgtcta gaaagaccag
    48121 gggtttttgg tattcttcaa aggcagaaat ctgagctcgg ctaaattttc caatgcagag
    48181 aatcccaatc acttcctcgc ttaaggtaaa aggatggtca ttaaaatagc gcaagatatc
    48241 atagtccaac tcttgggctc ttttttctat tcctaggcga atctggtagt agtagaggtc
    48301 gtccagctcc ccttgttcgc tgacccattg tataatggca atcttttgct tgggtttatg
    48361 ggactcgcct gtcttgaggt gcttggtgta gcccagctct tcaacaacgg ttaaaatacg
    48421 gtgtctggtt tcttctgtaa cagataggct ctggtcgcgg ttgaggacac gggatacggt
    48481 cgcgatagag acagaggcta gctgtgcaat gtcttttaag gtagccataa atcctccttg
    48541 attaggttag tatatcatat ttttctgctt tttactgata ttttagtaaa attttagtaa
    48601 aaaggattga ccttgggaaa tcccttggat acaatagaag aaaacgatta cacgttaagg
    48661 tggcttaacg gacagtcaaa ggagaaatca tatgacacaa catcttactg ctgaaactct
    48721 tcgcaaagac tttcttgctg tttttggtca agaagcagac caaactttct tttcaccagg
    48781 tcgtatcaat ttgattggtg aacacacaga ctacaacggt gggcacgttt ttcctgctgc
    48841 tatttccttg ggaacttacg gtgcagctcg taagcgtgac gaccaagttt tgcgtttcta
    48901 ctcagctaac tttgaggaca agggcattat cgaagtgcct ctcgctgacc tcaagtttga
    48961 aaaagagcac aactggacca attatccaaa aggtgtcctt catttcttgc aagaagctgg
    49021 gcacgtgatt gacaaaggtt ttgattttta tgtttatggg aatataccaa atggtgctgg
    49081 tttgtcttct tcagcatcct tggagctctt gacaggagtc gtggcagagc atctctttga
    49141 tttaaaatta gagcgtctcg atttggttaa aatcggaaaa caaacagaaa acaactttat
    49201 cggagtcaac tctggtatca tggaccagtt tgctattggt atgggggctg atcaacgtgc
    49261 tatttacctt gataccaaca ccttagagta tgatttggtg ccacttgatt tgaaggacaa
    49321 tgtcgttgtt atcatgaaca ccaacaaacg ccgtgaattg gcggactcta aatacaatga
    49381 acgtcgtgct gagtgtgaaa aagcagtgga agaattgcaa gttgccttgg atattcagac
    49441 cctgggtgaa ttggacgagt gggccgttga ccaatatagc tatttgatta aagatgaaaa
    49501 tcgtttgaaa cgtgctcgcc atgctgtgct tgaaaaccaa cgtaccctta aagctcaagc
    49561 agcccttcaa gcaggtgatt tggaaacatt tggtcgtttg atgaatgcgt cacacgtttc
    49621 tctggagcat gattataaag taactggttt ggaattagat acccttgttc acacagcttg
    49681 ggcacaagaa ggagttctcg gtgctcgtat gacaggggct ggttttggtg gctgtgccat
    49741 tgccttggtt caaaaagata ctgttgaggc ctttaaggaa gctgtaggca aacactacga
    49801 ggaagtagtt ggatacgctc caagcttcta tatcgctgaa gttgcaggtg gcactcgcgt
    49861 ccttgactag tcaaaaggag gatctatagt gaccttagta gataaatttg taacacatgt
    49921 catttctgaa agttcatttg aggaaatgga tcgaatctac ctgaccaatc gtgttttggc
    49981 acgagtggga gaaggtgttt tggaagttga gaccaatctg gataaattga ttgacctcaa
    50041 ggaccagcta gttgaggaaa ccgttcgatt agagacgatt gaggatagtc agactgcgcg
    50101 tgaaatcctt ggtactgaac tgatggactt ggtgactcct tgtccgagtc aggtcaatag
    50161 tgacttctgg gaagcctatg cctactctcc tgagcaagcg atagaggatt tttatcaact
    50221 cagtcagaaa aatgactaca tcaaactcaa ggccattgct aaaaatatcg cttatcgtgt
    50281 tccatctgat tatggagaac ttgaaattac catcaatctc tctaagcctg aaaaggatcc
    50341 gaaagagatt gcggtagcca agttggtgca agctagtaat tatcctcagt gtcaactttg
    50401 tttagagaat gagggttacc atggtcgggt caaccaccca gctcgcagca accaccggat
    50461 tatccgtttt gaaatggttg gtcaggaatg gggtttccag tattcgccct atgcttactt
    50521 taatgagcat tgtatctttt tagatggcca gcatcgtccc atggccatta gtcgtcagag
    50581 ttttgaacgt ctgttggcta tcgtagagca gtttccagga tattttgctg gatctaatgc
    50641 cgacctgccg attgtggggg gctctattct aactcatgat cattatcagg gaggccgtca
    50701 cgtatttcct atggaattgg ctcccttgca aaagaccttc cgatttgctg gttttgagca
    50761 ggtcaaggct ggaattatca agtggccaat gtctgtccta cgcttgactt cggattccaa
    50821 agaggatttg attaacttag ctgataagat tttccaggaa tggcgtcagt attcagattc
    50881 tagtgtgcag attttagcag agacagacga gacaccgcat cacaccatca caccaatagc
    50941 gcgtaagcgt gatggacagt ttgagttgga cttggtcttg cgagacaatc agacttcagc
    51001 agagcatcct gatggcatct atcatcccca caaggatgtc caacatatca agaaggaaaa
    51061 tatcggcttg attgaggtca tgggcttggc aatcttgcca ccacgtctga aagaagaagt
    51121 ggagcaagtc gctagctatc ttgtaggaga agctgttaca gttgccgatt atcatcagga
    51181 gtgggcagac caactcaaat cccaacatcc agacctaacg gataaagaaa aagcccttgc
    51241 aatcgtcaag gactctgtgg gtgctatctt tgtgcgtgta cttgaggatg caggagtcta
    51301 caagcagaca gaacaaggtc agacagcctt tatgcgcttt gtggaacagg tcggaatttt
    51361 actagactag gagctttctc cttgcacagt cctataaaaa gtagtatcat agtgtcgttt
    51421 gaaatatcag gaggaaacga tgaaagactt tcattttgac gctatatctg cctttgaaaa
    51481 ttacgaaatt gaaaaaatga gagatggtca cgttgtggtg acgaccaaag tagtgaactc
    51541 gtcgctcaac tactatggca atgcccatgg tggctatcta ttcacgctat gtgaccaaat
    51601 cagtggtttg gtggttatct cgctgggact tgatggggtg acactccaat cctctatcaa
    51661 ctaccttaag gcaggaaaac tcgacgatgt attgaccatt aaaggagaat gtgtccatca
    51721 aggtcgtaca acctgtgtaa tggatgtgga tatcaccaat caagaaggca gaaatgtctg
    51781 caaagcaacc tttaccatgt ttgtcacagg ccagcggtca gaagacagac aggtaaggat
    51841 ataaagagta atttaataga gtgaggtgaa ttttggaatt tataagattt gccttactca
    51901 ttttttgata ttgatatgat ttaattctaa aatagaaaat ttagtaatgt ggtagaaaat
    51961 acaagagttg tgttttaatt tctatggtat aattaaaagc atgaagataa aagaaataaa
    52021 gaaagttact ttacaaccgt tcacgaaatg gacaggtggt aaaagacaat tattgcctgt
    52081 tattagagaa ttaataccta aaacctataa caggtatttc gaaccttttg ttggaggtgg
    52141 agctttattt tttgatttgg ctcctaaaga tgcagttatt aatgatttta acgctgaact
    52201 aataaattgc tatcaacaaa ttaaggacaa tcctcaagaa ttgattgaaa ttttgaaagt
    52261 tcatcaggaa tataattcaa aagaatatta tttagattta cgttctgcag atcgtgatga
    52321 aagaatagat atgatgtccg aagtacaaag agctgcacgt attctatata tgttgagagt
    52381 gaactttaat ggtctatatc gtgtgaattc taagaatcaa tttaatgttc catatggacg
    52441 ttataagaat cctaaaattg ttgatgagga attgatatct gctatttcag tttatataaa
    52501 taacaatcaa ctagaaatta aagtgggaga ttttgaaaag gcaattgtag atgttcgaac
    52561 aggagatttt gtgtattttg accctccata tattccattg tctgagacga gtgcatttac
    52621 gtcttatact catgagggat tctcttttgc agatcaagta agattaagag atgcctttaa
    52681 gagattgagt gatacaggag cttatgttat gttatcaaat tcttctagtg ctttagtaga
    52741 ggagttgtat aaggatttta atatacatta tgttgaagct acccgaacta atggagcaaa
    52801 atcttcaagt cgaggaaaaa tttctgaaat tatagtcaca aattatgaaa aataacgaat
    52861 ataagtatgg aggtgttctt atgacaaaac catactacaa taaaaataag atgattcttg
    52921 ttcattcaga tacgttcaag ttcttatcaa aaatgaaacc agaaagtatg gatatgattt
    52981 ttgctgatcc accttatttt ttaagtaatg gtggaatatc taattctggg ggacaagtag
    53041 tttctgttga taaaggagat tgggataaaa tttcttcatt cgaagaaaaa catgagttta
    53101 atcgtaaatg gattcgccta gcaaaagaag ttctgaagcc taatgggacg gtatggattt
    53161 caggtagttt gcacaacata tactcagttg gaatggcatt agaacaagaa ggttttaaaa
    53221 ttctgaataa tattacttgg cagaaaacaa accctgcccc caatttatct tgtcgttatt
    53281 ttacccattc tactgaaacc attttatggg ccagaaaaaa tgataaaaaa gctcgtcatt
    53341 actacaatta tgatttaatg aaagaattga atgatggaaa acaaatgaaa gatgtctgga
    53401 ccggttcttt aacaaagaaa gttgaaaaat gggctgggaa acatccaact caaaaaccag
    53461 agtatttgtt agaacgtatt attttagcct ctactaaaga gggtgactat attctagacc
    53521 catttgttgg tagtggcact acgggtgttg ttgcgaagcg gttaggtaga agatttatag
    53581 gtatcgatgc tgaaaaagaa tatttaaaaa ttgcaaggaa gaggttggag gcagaaaatg
    53641 aaacaaacta gaaattttga tgagtggttg tcaacgatga ccgacacagt agctgactgg
    53701 acttattata cagattttcc taaggtttat aaaaatgtat ctagtataaa agttgctcta
    53761 aatattatga atagtcttat tggtagtaaa aatatacaag aggatttttt agacttatat
    53821 caaaattatc cagaaatttt gaaagttgtg cctctactta ttgctaaacg tttgagagat
    53881 acgattattg ttaaggatcc gataaaagat ttctat
//
LOCUS       DAVMAF010000005        52127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMAF010000005 DAVMAF010000000
VERSION     DAVMAF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.005"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(247..609)
                     /locus_tag="VYO97_000270"
     CDS             complement(247..609)
                     /locus_tag="VYO97_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1753141.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            623..697
                     /locus_tag="VYO97_000271"
                     /pseudo
     CDS             623..697
                     /locus_tag="VYO97_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(945..1082)
                     /locus_tag="VYO97_000272"
                     /pseudo
     CDS             complement(945..1082)
                     /locus_tag="VYO97_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1460..2149)
                     /gene="rplA"
                     /locus_tag="VYO97_000273"
     CDS             complement(1460..2149)
                     /gene="rplA"
                     /locus_tag="VYO97_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1753142.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2357..2782)
                     /gene="rplK"
                     /locus_tag="VYO97_000274"
     CDS             complement(2357..2782)
                     /gene="rplK"
                     /locus_tag="VYO97_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1753143.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2914..3051
                     /locus_tag="VYO97_000275"
     CDS             2914..3051
                     /locus_tag="VYO97_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753144.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEIADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(3091..3807)
                     /locus_tag="VYO97_000276"
     CDS             complement(3091..3807)
                     /locus_tag="VYO97_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1753145.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKPKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVVNKGDYYSIQGKYDEIVIANKHYPLSKDYNPGENPTAKAELLKLI
                     AAMQAAGYPISDHYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3825..4286)
                     /locus_tag="VYO97_000277"
     CDS             complement(3825..4286)
                     /locus_tag="VYO97_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1753146.1"
                     /translation="MCLICQRIELIKAGENPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KEHVTELHHMENPVKFRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     TGDMKGHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKHLA"
     gene            complement(4288..5325)
                     /locus_tag="VYO97_000278"
     CDS             complement(4288..5325)
                     /locus_tag="VYO97_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1753147.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEEHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGKTVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDEKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEERIDLPMTTHFAFSVFEE
                     VGHGANSNIPKEVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATELM
                     VDAYLKSEGVS"
     gene            complement(5423..6748)
                     /gene="brnQ"
                     /locus_tag="VYO97_000279"
     CDS             complement(5423..6748)
                     /gene="brnQ"
                     /locus_tag="VYO97_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1753148.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6919..7011)
                     /locus_tag="VYO97_000280"
     CDS             complement(6919..7011)
                     /locus_tag="VYO97_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1753149.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(7054..7206)
                     /locus_tag="VYO97_000281"
     CDS             complement(7054..7206)
                     /locus_tag="VYO97_000281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1753150.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7366..7944)
                     /locus_tag="VYO97_000282"
     CDS             complement(7366..7944)
                     /locus_tag="VYO97_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1753151.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7998..9398)
                     /gene="pepV"
                     /locus_tag="VYO97_000283"
     CDS             complement(7998..9398)
                     /gene="pepV"
                     /locus_tag="VYO97_000283"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1753152.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSSWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9411..10016)
                     /locus_tag="VYO97_000284"
     CDS             complement(9411..10016)
                     /locus_tag="VYO97_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1753153.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10187..10987
                     /locus_tag="VYO97_000285"
     CDS             10187..10987
                     /locus_tag="VYO97_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753154.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNTLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11169..12002)
                     /locus_tag="VYO97_000286"
     CDS             complement(11169..12002)
                     /locus_tag="VYO97_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1753155.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKDQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGEYGLEHPQEIQLMRMTQFLMERMDPE
                     TIVWLRSLPLLEKKEVDGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11992..13836)
                     /gene="uvrC"
                     /locus_tag="VYO97_000287"
     CDS             complement(11992..13836)
                     /gene="uvrC"
                     /locus_tag="VYO97_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1753156.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNSPSKVCF
                     YYHIGQCMAHTICKKDEVYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDQEAVQALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQRDGLTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVELSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13900..14577)
                     /locus_tag="VYO97_000288"
     CDS             complement(13900..14577)
                     /locus_tag="VYO97_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1753157.1"
                     /translation="MRWLFRLIGAFFFFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANL"
     gene            complement(14656..15888)
                     /gene="murN"
                     /locus_tag="VYO97_000289"
     CDS             complement(14656..15888)
                     /gene="murN"
                     /locus_tag="VYO97_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1753158.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTIGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15893..17113)
                     /gene="murM"
                     /locus_tag="VYO97_000290"
     CDS             complement(15893..17113)
                     /gene="murM"
                     /locus_tag="VYO97_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1753159.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17225..18004)
                     /locus_tag="VYO97_000291"
     CDS             complement(17225..18004)
                     /locus_tag="VYO97_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1753160.1"
                     /translation="MAVMKIEYYSQVLDMEWGMNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(18073..19734)
                     /locus_tag="VYO97_000292"
     CDS             complement(18073..19734)
                     /locus_tag="VYO97_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1753161.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19909..22131)
                     /gene="recJ"
                     /locus_tag="VYO97_000293"
     CDS             complement(19909..22131)
                     /gene="recJ"
                     /locus_tag="VYO97_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1753162.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIAFMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22408..23166
                     /locus_tag="VYO97_000294"
     CDS             22408..23166
                     /locus_tag="VYO97_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1753163.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNIYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            23177..23971
                     /locus_tag="VYO97_000295"
     CDS             23177..23971
                     /locus_tag="VYO97_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1753164.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDHSILSGYTSKRTALLDDSFKPSD
                     YSIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23984..24661
                     /locus_tag="VYO97_000296"
     CDS             23984..24661
                     /locus_tag="VYO97_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1753165.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24671..25330
                     /locus_tag="VYO97_000297"
     CDS             24671..25330
                     /locus_tag="VYO97_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1753166.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25579..26769
                     /locus_tag="VYO97_000298"
     CDS             25579..26769
                     /locus_tag="VYO97_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1753167.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKKVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(27090..27971)
                     /locus_tag="VYO97_000299"
     CDS             complement(27090..27971)
                     /locus_tag="VYO97_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1753168.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(28112..29440)
                     /gene="vncS"
                     /locus_tag="VYO97_000300"
     CDS             complement(28112..29440)
                     /gene="vncS"
                     /locus_tag="VYO97_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1753169.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKYYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(29437..30093)
                     /gene="vncR"
                     /locus_tag="VYO97_000301"
     CDS             complement(29437..30093)
                     /gene="vncR"
                     /locus_tag="VYO97_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1753170.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDSVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30142..30225)
                     /gene="pep27"
                     /locus_tag="VYO97_000302"
     CDS             complement(30142..30225)
                     /gene="pep27"
                     /locus_tag="VYO97_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1753171.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(30405..31784)
                     /gene="vex3"
                     /locus_tag="VYO97_000303"
     CDS             complement(30405..31784)
                     /gene="vex3"
                     /locus_tag="VYO97_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1753172.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31836..32483)
                     /gene="vex2"
                     /locus_tag="VYO97_000304"
     CDS             complement(31836..32483)
                     /gene="vex2"
                     /locus_tag="VYO97_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1753173.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32496..33773)
                     /locus_tag="VYO97_000305"
     CDS             complement(32496..33773)
                     /locus_tag="VYO97_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753174.1"
                     /translation="MNPIQRAWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGSGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(34130..34300)
                     /locus_tag="VYO97_000306"
                     /pseudo
     CDS             complement(34130..34300)
                     /locus_tag="VYO97_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(34443..35042)
                     /locus_tag="VYO97_000307"
     CDS             complement(34443..35042)
                     /locus_tag="VYO97_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753175.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(35179..36063)
                     /locus_tag="VYO97_000308"
     CDS             complement(35179..36063)
                     /locus_tag="VYO97_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1753176.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(36067..36453)
                     /locus_tag="VYO97_000309"
     CDS             complement(36067..36453)
                     /locus_tag="VYO97_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1753177.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(36446..37789)
                     /locus_tag="VYO97_000310"
     CDS             complement(36446..37789)
                     /locus_tag="VYO97_000310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1753178.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(37876..38760)
                     /locus_tag="VYO97_000311"
     CDS             complement(37876..38760)
                     /locus_tag="VYO97_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753179.1"
                     /translation="MIQARNKLSQEELSGAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(38772..39389)
                     /gene="cysE"
                     /locus_tag="VYO97_000312"
     CDS             complement(38772..39389)
                     /gene="cysE"
                     /locus_tag="VYO97_000312"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1753180.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHSFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(39405..41618)
                     /gene="pnp"
                     /locus_tag="VYO97_000313"
     CDS             complement(39405..41618)
                     /gene="pnp"
                     /locus_tag="VYO97_000313"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1753181.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(42303..43169)
                     /gene="metF"
                     /locus_tag="VYO97_000314"
     CDS             complement(42303..43169)
                     /gene="metF"
                     /locus_tag="VYO97_000314"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1753182.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISITAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(43233..45482)
                     /gene="metE"
                     /locus_tag="VYO97_000315"
     CDS             complement(43233..45482)
                     /gene="metE"
                     /locus_tag="VYO97_000315"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1753183.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDTDYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(45743..46009)
                     /locus_tag="VYO97_000316"
     CDS             complement(45743..46009)
                     /locus_tag="VYO97_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753184.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            complement(46135..46538)
                     /locus_tag="VYO97_000317"
                     /pseudo
     CDS             complement(46135..46538)
                     /locus_tag="VYO97_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            46694..47695
                     /locus_tag="VYO97_000318"
     CDS             46694..47695
                     /locus_tag="VYO97_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1753185.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(47763..50165)
                     /locus_tag="VYO97_000319"
     CDS             complement(47763..50165)
                     /locus_tag="VYO97_000319"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1753186.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(50242..50751)
                     /locus_tag="VYO97_000320"
     CDS             complement(50242..50751)
                     /locus_tag="VYO97_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753187.1"
                     /translation="MIIKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(50751..51797)
                     /gene="pheS"
                     /locus_tag="VYO97_000321"
     CDS             complement(50751..51797)
                     /gene="pheS"
                     /locus_tag="VYO97_000321"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1753188.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNICKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT        14728 a        11841 c         9824 g        15734 t
ORIGIN      
        1 attttagatt cagtctacta tacataaact aacctcctca ttattagcaa ggattctact
       61 gattataacc taattcttag actacttcaa gactaactgt ccaataataa tctacagata
      121 aatcatccac tcttaataaa attttattca atccatctac tcattcccaa aaggttgctg
      181 tataatgcaa gacatttttc ttactgattt tacttttcct taccctcaat ttagtgagct
      241 aataaattat tcttcttcaa aaggttgaga tttatctagt aactctaaaa cattaatggt
      301 aggtagcaac attgcttcac tgctatcaat tgccgaaact attgaaacaa tagaacgtaa
      361 ataatgataa agaatagcag taccatttac gtatagaata tcggtgatat tttcatctat
      421 atttatttca aaatatccta ttacagttac agatattttt ttcatggcat ctttgtcaac
      481 taaagaaaca ctaagtttta cggctccatg actataatca tctgtatgac caaccttaac
      541 tatgggatat aatatatcga aggtaaatgt gaacaaactg attaggaaat ttaaaccaac
      601 ttctaacaat ctgttatact caatgaaaac caaagagcaa actaggaaac tagcctcagg
      661 ttgctcaaaa cactgttttg aggttgtaga tggaactgac gtagtcagct caaaacactg
      721 ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat acgcacggca aggcgacgtt
      781 gacgtggttt gaagagattt tcgaagagta ttagaaagct cgatggacta ttccagattc
      841 attccactag atttcgaaat cgagtagggg ataatctcta gcccctctca caccaccgta
      901 cgtgccgttc ggcatacggc ggttcaacta actgttaacg catgtcattc aaggtaataa
      961 tccaaacacg aaaccagtcc acgtttttca aggactggtt ttgatatagc acgtttaagt
     1021 accgacttct gagctactaa ttgatagtgg tcgccccagc cagatacctt atccgctata
     1081 taggccttat tatcacgttc ctgttcgtag agccacgatt tcgctatccc ttcctctgac
     1141 gattcttatt tccagcgtca gacttaaaac ggtctatccc cagaccgttt taatccgcta
     1201 cctcacgata gtcagacttg ggaatcgcta ttgggttcac cggtagcagg tgcccaccga
     1261 ggacttacac ctcaaatata taataaaatg aaataagaat aggacgaatc gatcaggaca
     1321 gtcaaatcaa tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatcta
     1381 ttatacaaca tgcccgtcgt acatcaaaaa aagttgagac atagcgtctt caactttttt
     1441 acattacttt aactactgat taaagtgagt ttacgtcaac tttgatacca acaccttgag
     1501 tagttgtgat agtcaagttt gttacgtaag ttcctttagc tgtagctggt tttgcttttt
     1561 ggattgtttc gttgaaagct ttaaagtttt caaccaattt ttcagcttca aatgatactt
     1621 taccgatgat tgcttgaacg ttacctgcac ggtcagcacg gtaagtgatt ttaccacctt
     1681 tagactcttc aactgcttta gcaacatcca ttgttacagt accagtttta gggtttggca
     1741 tcaagttacg tggtccaagg acacgtccaa gacgtccaac aagagccatc atatcaggtg
     1801 tagcgataac tacgtcgaag tccaaccaac cgtcgttgat tttagcaaca aggtcatctt
     1861 caccaacaaa gtctgcacca gcagcttttg cttcttcagc ttttgcacca cgtgcgaaaa
     1921 caagaacacg tgaagtttta ccagtaccgt ttggcaatac cattgctcca cggatttgtt
     1981 ggtcagcttt tttaacgtcg atgttcaagt tgtaagcaac ttctacagtt gcatcaaatt
     2041 ttgcaaagtt agtttctttt gcaagtgcta cagcttcttc tacactgtat gcttttgtgc
     2101 tgtcgatttt ctcaagagca gcacgaagtt gtttgctttt tttagccatt ttctattctc
     2161 cttgtaagtg gttcaatcga ttttcatctc ccacgtcact tctcgaaatg atgaagtctt
     2221 gcgggttttc agtctactat ttttctcttc ctacggcctt aagctccctt gctgtaccca
     2281 agtacaagcc tcagtcactt gcctagtatg aaaagcaact aatagactgc tgtgagattt
     2341 tctactgtgt tattgattag tcaacaacag tgaatcccat agaacgagca gtaccttcga
     2401 tcatacgcat tgcagactct acgtttgctg cgttcaaatc tggcatctta gtttctgcaa
     2461 tttcttgtac ttgtgcacga gtaactgtag caactttagt tttattaggt gtacctgatc
     2521 ctttttcaac acctgcagct tttttcaaaa gaacagcagc tggtggtgtt ttcgtaacga
     2581 aagtaaatga tttatcttcg taaactgaga taacaactgg aatgatcata ccagcttggt
     2641 cagctgtacg agcgttgaac tcttttgtga atcccatgat gttgatacca gcttgaccaa
     2701 gagcaggtcc aaccggtgga gctggtgtag ctttaccagc agggatttgc aattttacaa
     2761 gtttttcgac ttttttagcc atttttaaat cctcctttgt ggttttggcg gtaattagag
     2821 atttttacct cccacaagta tgtttttcac atacccctcc attatacact atttatctgg
     2881 aattgcaaga gaaaagttta ttttttatac tcaatgaaaa tcaaagagca aactaggaag
     2941 ctagccgcag gctgctcaaa gtacagcttt gagattgcag ataaaactga cgaagtcagc
     3001 tcaaaacact gttttgaggt tgtgaataga accgacgcag tcagtaggta aggcgacgct
     3061 gacgtggttt gaagagattt tcgaagagta ttaatcgacg tagtctccgc cttcaaagcc
     3121 atagtactct tccaaactga ggccacttgc agcaatttct ttggcttctt ttccaacgta
     3181 gcggagatgc cattcttctg ccatgtagcc ggtctccttt tccttgcctt tgagatagcg
     3241 gacaacaaag ccatagtcag ccgcatggtc caagagccac tgggctgcct tttcttcagt
     3301 aactaaatca ccatctgtcc caataaggtc aaaagcaagg cctgtttggt gttcactata
     3361 acctgggcgt gctgagtagc gatccgctgc ttccttacca tcttgattga catagtcttg
     3421 gtagagctta gcctgagttt cataacttct aaaaccactg tagtgatcgc tgattgggta
     3481 gccagctgct tgcattgctg caatgagttt cagcaactct gccttggctg ttggattttc
     3541 tcctggattg tagtcttttg acaaaggata gtgcttatta gctatgacga tttcatcgta
     3601 tttcccttga atactatagt agtctccctt attgacaact tctgcctttt tctgactaga
     3661 tgcttgagat ttgctaccta cagttccttc cggttgactg ctttgctccg tcttttgagt
     3721 attttcttca actttcggtt tttcttgcga acaagctgca agagtcaatg ctagtaaacc
     3781 agataaaatg acatatcttt ttttcatttc tttcttcctc tctcttaggc caaatgcttc
     3841 tccaattcat gagataagat tttaatcatc tcttccagct ctggcgattg tacttggcaa
     3901 tcttctgctg ccatttcttc ccagggcacc caccagactg ggccacggcc attcaatcca
     3961 tgacccttca tatcacctgt tcttcgtgga aagagatgcc aatgagcgtg ggcatcacca
     4021 tttcctagga gttcaatatt catcttttca gcctcaaatg ctttggcgac tgcttcttgg
     4081 accaaactca tttcctctaa aaagcgaaat tttacaggat tctccatatg gtggagttct
     4141 gtaacatgct cttttgctaa aaataaggta tagcccttga agtattggtg atccccaata
     4201 accacatagc ctgtttctag ttctttaaca aagtagggat tttctccagc tttgatgagt
     4261 tcgattctct gacagattaa gcacattcta tgataccccc tcgctcttaa gataagcatc
     4321 aaccataagc tcagtcgcta ctactgaatc gatgtgggtg cgctcataag agtgactgga
     4381 ctcaatacca gctccaagaa gtgcatgctt gacctccgcg cctgctgaca tcgctgctga
     4441 agcatccgaa ccgtaaaatg gataaatgtc gagcttaaat ggaatatctt gctccttcgc
     4501 caaagctact aagtgttgac ggaagtcata gtgataagga ccagaagcat ccttgacaca
     4561 gatagatact gtgtactcat ccgtctgttg gtcatccccc attgcaccca tatcgacagc
     4621 caggtactct accacctctt ttggaatatt tgaattggca ccatggccca cctcttcaaa
     4681 gactgaaaag gcaaaatgtg tcgtcatcgg caagtcgatt ctctcttctt tataaactcg
     4741 aaggagattg agcagaattg ctgcgctgac tttgtcgtcc aagtgacggg acttgataaa
     4801 accagtctct gtcacgacag tacgtggatc aaagctgata aaatcaccga cctcgatgcc
     4861 caaggcacgc gtttcttttt cattggtcac tttttcatcc aaacgcactt ccatattgtc
     4921 ctgagtgcgt tcagcagttc ctgcatcttt gtagacatgg caagatgtct ggtggatgag
     4981 gatcgttcca gataccgttt tacctgtact agctacatga acagtacagt tttctccctc
     5041 aatcatgttc caaggaaaac caccgatacg gtccaatttg agacgaccat ctggcttgac
     5101 tgcacgcaca atggctccaa gtgtgtccac atgggcagtt acatagcgat gttcttcgtc
     5161 gttttgacct ttgatggtca cattgacacc gcccttggct gttcgaacag gctggtaacc
     5221 aaaaccttct agagtcttga ctaaataatt cgaaatttca cgagtaaagc ccgttggcga
     5281 tgcgatggct gtcagttctt tgatatattc tactgtttga ttcatttctt cacctctttc
     5341 gctacctatt ataacacatt tatcatcgga taaaaaaaga aaatcactcc tgtagacttg
     5401 gctacaaaag tgatttaagt gattattcca tttcaaaaac atcgctttct tgcttgtttg
     5461 gtagaaccaa tgagagcaag attccaacaa tggctggcac caaccatggg agagatgcct
     5521 tggcaaaagg aagaacgcta acaagatttg caagaaactc aaccttaaac gagcttccta
     5581 gtacgcttgc aatggcaata actgtaacca cagcaattgt caactgcata cctggttttg
     5641 aaagggcaac aaatttgttg acaatgacaa tcataacgat agcaatcgtg attgggtaca
     5701 agataaccag tactggaatt gagtacttga taatcgcatc aagacccaaa ttggcaatag
     5761 caaatccaat caaggtaaag gctgtcgcat aaaccttgta gctgatttgt gggaagcgct
     5821 cattaaagaa ctcagctgtt gacacaatca aaccaacagt cgttgtgaag caggttacgg
     5881 taaccatagc tgcaaggaag agttgagctg ttgagccaaa gatttcttga gtggcttgtg
     5941 acaagatgta aacacctggt gttccaccct tcatcgcttc agctggtact gggaaatgat
     6001 ttccaagaaa acctaaaccg atgtaaagag cgctgaaggc aagggcaaca acgataccaa
     6061 caacccaaat agttgaaatg tattctttct tacttgaaaa tccaagttgt ttcaaggttt
     6121 gaactgcgat tacgctaaag gccactgagg caagggcgtc caaggtattg taaccttcta
     6181 ggaaacctgt accaaaggca gaagcttgat aagcagctga agcagcttga ggacttgttc
     6241 caccatattt gatagctccc agaacgacca agataacaat caaaattgca aagactggcg
     6301 ttaaaatacg tccaatgcgg tctaagattt ttgatggatt aagcgaaatc aaataggctg
     6361 ccgcaaaata cagaaccgta aatacaatca agccaagtcc tttatttgca tccgacaaaa
     6421 gggggctaat ccctacttcg taagctgttg tagcagtacg tggggtagca aagaatggac
     6481 cgattgacaa gtaaagaact gagaggtaaa gagtcgcaaa ccaaggcgct atcttcgttg
     6541 aaatctcgta gatatatcct ttaggattta gcgttccaat aataagggtc aagacggcga
     6601 taccaacgcc tgaaaagaca aaacctgcga tggcaggaag aaaatgttct ccagatagag
     6661 cacctagaga aggcggaaaa atcaagttcc ccgcaccaaa aaatattcca aacaggagca
     6721 aacctgttag ggtacctttt ttagccataa aaatctcctt catatttata aaatactgac
     6781 ctagtatacc acgattagga gtctgaaaaa agcatcacga ggcaaatatt aaatgttttc
     6841 aattttctta aaaataagaa ttatctaaaa ataaaactct cctacccagt ccacaccaag
     6901 taggagaaat gcaaaatttt aaagttcttc aaaggctaac atgccaccct ggacattgat
     6961 aacattataa ccttgttcta acaggaattg gcaagcacgc gccgatctca ttccagattt
     7021 gcaaataata taatgcaagc gatctttgtc caattaatca taactatcag ccaattgact
     7081 aagcggtagg ttgtgggcac cttctaaatg aagagctgca aactcatcca cttctctcac
     7141 atccactaga gaaagctggt cgttttggta aagctggtaa aatgcgtcaa aggctatttc
     7201 tttcattctt tttcttctaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
     7261 taccgtgcgt atgttaccga cttcgtcagt tttatctgca acctcaaagc tgtactttga
     7321 gcagcctacg actagcttcc tagtttgctc tttgattttc attgactata aaatggtttt
     7381 aattcttttt ttcaaatctg gcactacttc tgcctcaaac caaggatttt tggccatcca
     7441 gatttgattt cgtggtgatg ggtgaactag cggaaaatag gctggcagat agtctttata
     7501 gtgtttcacc ctctccgtta ccttcccact gattttctcc tgtaaatagt aggcttgggc
     7561 atattgccca atcaagaggg ttaactgaat atcaggcaat tcctgtaaga gctgcggatg
     7621 ccatttttct gcaaaacctg tacgaggcgg aagatcaccc gacttgccat gtcctggaaa
     7681 gtagaaatcc ataggcaaaa cagcaaaata acctgaattg taaaaggtat cttcatccac
     7741 acctagccag tcccgcaagc ggtcaccact tttatctttc cagtaaaggc ctgcttcttg
     7801 agttttaagt cctggagcct gaccgatgat attgatgcga gcagtttttg gcgctgcaaa
     7861 gagaggctca atgccacgct ctgtatagct ggcattctgt gaatccgcca tgatagcctg
     7921 tttgattctt tcgatttgag acatctactt tccctccaaa aagaagtcca agcataagat
     7981 ctcagacttc tatcgtttta tttgatcaat tcgtaaatag cttcggcata aattgctgct
     8041 gctcggaaga gatcgtccaa ggcaataaat tcattggctt ggtgcatggt atcaattgag
     8101 tctgggaaca tagcaccata ggcaactccg cgctctaaca agcgaccaaa ggttccacca
     8161 ccgatgactt gttcatgacc tttaaggcct gtttgttttt catagacatt caacaaggtt
     8221 tgcacaagtg gatcttccat tggcacatag tgaggggtat gaccatgttc tgatagactg
     8281 acagaagcaa ctggcaagtt ttctaggatt gacttgattt gttctggact tgttcctttt
     8341 ggatagcgga tgttaagggc aatggtatta tcagcacttg tttcatcgaa gcggaagacg
     8401 ccggcattca tagaaagagc acccatcttt tcatccacat gagcaatctt gagattttca
     8461 ccctcatgat cgttcaagag aattttacct gcgatgtcaa ggtagtcttt ggctggacca
     8521 gcaaagccaa actggctgag gaagagggct aggtaagtcg caccattgac acctgaagca
     8581 ggcatagcac cgtgggctga tttaccaatg atcgtcacct tgtatttgcc agcttcttct
     8641 tggagttctc ctctaagttt gtgttctgca acaaaggcat ctagtttagc ttgcaagtca
     8701 gccaaatcac ctgaaacgac tgctgttgct gattctggta ccatattttc acgtaaacca
     8761 cctgtaaagc tgtgaagacg ggcagcacct gtattttctc ctgcaaagtg gaggtattcc
     8821 gtgatatttc ctttttcacc attgatgatt ggaaattcag catctggtga gaaaccgaaa
     8881 tctggtttgg caagtcctac gtgctcaaag tagtagtcca tgtctgccca gcctgattct
     8941 tcgtctgttc caacgatgaa gcgaactttc ttagaagttg gaagacccaa ttctttgatg
     9001 attttcaaac catagtaaca agctgttgta ggacccttat cgtccgaagc cccgcgcgca
     9061 taaaggcgac catctttgat agttggtgtg taaggatctg tatcccaact gctaccagct
     9121 ggaaccacgt ccatatgggc aaagattccg agaacttctt ctccatcacc aaactcaaaa
     9181 tgtcctgcat agttatcaac atttttagtt ggataaccat cacggtctgc gatttcaagg
     9241 aatttttcca aggcttttac tggaccaggt ccaaatggat gttgggcatc agccttgcta
     9301 tcatcacgtt ctgagttgat ttccaaaagg ctaaacaagt cagccaagag gtcttctttg
     9361 cgtttttcta cttctgctgt aaaatcaatt gctatcattt tttcttcttc ctatcttttt
     9421 tcgatgattt catctactgg caagcggtag cttggttcca atttgtcgtc tgtgtaaccc
     9481 actgtgatca agagttctgg gcggaaacgg tcttcgattt ccaaaacttc attaactttt
     9541 gatttgtcaa aaccaagaat aatgttagaa ccaattcctt ggtctgtaag agcaagaacc
     9601 aagttcatgg caaccaaacc tgcattgaga gctaggtagt cgctgacttg ttgttcattg
     9661 tagcgtgcaa attcagctgg caaatttttc atgaagtatt gaagttgttc ttctgagaaa
     9721 ttgttagcac caccaactcg ggcaatctta cgagcgcgtt tggcaaggtc tgtatctgta
     9781 aatagggcaa tggttacagg tgctgatgat acctgctcaa agtttgaacc ataagccaac
     9841 tttgccagtt cagcattttt ctcacgaaca actacaaatt tccaaggctg gctgttgtgg
     9901 gcgcttggcg ccaaggttgc aatttcgata gccgtacgca catcttttgg atcaacaggc
     9961 ttatcagtga aatgcttggt cgcatgacgt tttttattta attcaagaaa tttcataatc
    10021 gatttccttt tctaattcta ctgcttccat tctaccataa tttgaaagag cttgcaggga
    10081 tttttcatga ttaagttttt ctatcacaga cataaaagat atatattggt ttatcaggaa
    10141 catttctgat aaactagcaa gtactttaca tttgaatgga gacagtatga aaaaatttag
    10201 cctattacta gctatcctac catttttggt tgcctgtggg aatcaagcta cacccaaaga
    10261 gactagcgct caaaagacaa tcgtccttgc tacagctggc gacgtgccac catttgacta
    10321 cgaagacaag ggcaatctga caggcttcga tatcgaagtt ttaaaggcag tagatgaaaa
    10381 actcagcgac tacgagattc aattccaaag aactgcttgg gagagcatct tcccaggact
    10441 tgattctggt cactatcagg ctgcggccaa taacttgagt tacacaaaag agcgtgctga
    10501 aaaatacctt tactcgcttc caatttccaa caataccctc gtccttgtca gcaacaagaa
    10561 aaatcctttg acttctcttg accagatcgc tggtaaaaca acacaagagg ataccggaac
    10621 ttctaacgct caattcatca ataactggaa tcagaaacac actgataatc ccgctacaat
    10681 taatttttct ggtgaggata ttggtaaacg aatcctagac cttgctaacg gagagtttga
    10741 tttcctagtt tttgacaagg tatccgttca aaagattatc aaggaccgtg gtttagacct
    10801 ctcagtcgtt gatttacctt ctgcagatag ccccagcaat tatatcattt tctcaagcga
    10861 ccaaaaagag tttaaagagc aatttgataa agcgctcaaa gaactctatc aagacggaac
    10921 ccttgaaaaa ctcagcaata cctatctagg tggttcttac ctcccagatc aatctcagtt
    10981 acaataacac tcttcgaaaa tctcttcaaa ccacgtcagc tttatctgca acctcaaagc
    11041 tgtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc attgagtata
    11101 agatatacta aaagcgattg gaccagccaa tcgctttttt agtttgcatt ggctatttta
    11161 ggaaactctt acaaaaaatc aaaaaaattc ttcacatcct ctacataccc atgcttttct
    11221 attaagctgg ctaataattc cagattgtgc ccctgatagt tatcgtcacg acgcagttct
    11281 tcatacattt cgataaaggg aagccccttg gacttggcca attctaactc agcttcgtaa
    11341 tcataagcaa ctttacgaaa ttggatattg agtaattccc catcttcaac ttctatcacg
    11401 gcatactggg cacggtgatt ttttaacgcc tcccaattaa aatagggcat gccaatcgac
    11461 cctggattga tgatttgttg cccttgactt ccataacgaa gcaactgctt gtgaacatga
    11521 ccatagacgg ccacgtccgt ttccgcatct agcagttggt caaatttctc tgtatcattc
    11581 tcaactagca agtcaccacc atagttctta tttggcaaat tatgagagat agaaaagcgt
    11641 agcccgtcaa cttctttctt ttccagcaaa ggcaagcttc gtagccagac aatcgtttca
    11701 ggatccattc gctccatcaa aaactgggtc attcgcatga gctggatttc ttgcggatgt
    11761 tccaaaccat attcgccatc caaggcctca aggacacgat catcccaatt gcctcgaaca
    11821 cttgctgtga taggaaggtc ctttagcagg gcgaccaagt catttgcacc tggaccggga
    11881 agaaaaatat ctcccagaag ccagtattca ctgacccctt gatctttagc atctgcaatc
    11941 actgcttcta gcgccgtcgc attgccatga atatctgata aaattgcgat tttatggttc
    12001 attgtggttt ccttccgttt ggtaatctac tctttcttct gctacttgag gcaaggcttc
    12061 tccctgaggg ttcaactttc tctgaacagc ttctgctact gctctaggca caccaacttc
    12121 gacaatttca tccacactgg cttccttgat tttagttaga gacttgaaat gcttcatgag
    12181 attctgcttg cgtttaggtc ccagaccgtc aattccatcc agttgtgatg aaaaagaatt
    12241 tttggagcgc agttggcggt gaaaggtgat ggcaaagcgg tggacctcat cttggatgcg
    12301 ttggaggagg aaaaattcct gagaattgcg tgacaactcc actacctcaa gcggatctcc
    12361 aaagagcaat tcatgggttt ggtgcttgtc attcttttgc agacctgcaa tggggatatc
    12421 caagcccagt tcctcttgga tgacttgctt agcgatattg acttgacctt gtcccccatc
    12481 aatcacaatc aaatctgggg gagtcaaacc gtctcgttga actctaccat aacgtctgcg
    12541 aatgacctct cgcatactgg cgtagtcatc tggacctaca accgttttta tcttgtactt
    12601 acggtaatcc ttcttactcg gtttaccatt gacaaagacc accatggctg aaacagggct
    12661 agttcccatg atgttggagt tgtcaaaaga ctcaatgcga actggagttg gaatttgtag
    12721 aagtcgtcct aggttttcaa tagccccttg ggtcttttcg actgattttt ctagcaggtt
    12781 aaatttctgc tccagactaa cacgggcatt tttgatggct agattgacca gctgtttttt
    12841 ctctccacgc tggggtttga gaatcttagt atccaccaag gcctgaacgg cttcttgatc
    12901 aatatcctgc ggaatcagta cctcattggg aactaggtga gatttttctt gatagaattg
    12961 tcccacatag gtcaagaaat cctcatctgg atcattgaag taggggaaga gattgacatc
    13021 gcgctcgatg agcttgccct gacggacaaa gaaaacctgc acacacatcc agcccttatc
    13081 cacatagtag ccaaagacat cgcgattttg gagatcctta gccatgaccc gttgcttggt
    13141 tcgaagcgtt ccaatagcct gaatcaggtc acggtattcc gccgcacgtt caaactccat
    13201 agtctgggct gccgctgcca ttttgccctt tagctcatca atgattttgt tatcctgccc
    13261 tttcagaaaa tcagaaacct cctgggccat agacttgaaa taaacctcat ccttcttaca
    13321 gatggtgtgg gccatacatt ggccgatatg gtagtaaaaa cagaccttag agggcgagtt
    13381 ggtacatttt cgaaaaggga aaatcctatc tagaagtcgc ttgatttcat tggctgcccc
    13441 cacatctgga taagggccaa aataaagacc accgtccttt ttgacctgac gagtaatgat
    13501 aagccttgga tagcgctcat tggtgatttt gatgaaagga taggacttgt catccttgag
    13561 catgatattg tacttgggct tgttttcctt gatcaggttg atttctagga gaagtgcctc
    13621 aatattagac tccgtaacaa taaattcaaa atccacaatt tcagacacca gagcctctgt
    13681 cttggtatca tgacttccac gaaaatagga ccgtactcga ttacgcagat ttttagcctt
    13741 tcctacatag ataatggtgc catttttatc cttatgaatg tagcaaccag ggctggtcgg
    13801 caagagctct agttttgatt taatcaagtt gttcatagtt ccattatagc aaaaaaagta
    13861 gggaaagata gtaccctact cactagtctc atatattttt cagaggtttg caacatcctc
    13921 ttcctggata ccataaaagg aacctgctgg ttgcatgaca gacttctcat ctgtatggtc
    13981 caagccaatc gcatgaccta actcatgttc tgccgtatgg acaaggcgtt cataggagta
    14041 gccatagtat ggattggaca aataataatg attcaaccgc accgttacgg acaagaattg
    14101 ccctgttaag agattagttt gactttccgc ctctcctgcc acaggagtgc ctccgtcgtt
    14161 catctccgta gccgtaatat ccgccttact agactcagtc acgagttcaa agttaaaagc
    14221 accagtttga ttccagttct ggattgcttc taaataagcc tcttgaaagc gtgaatccat
    14281 ctgcggatcc aggtaaatac gagcagaggc ctgtgcccac cttccttcag aatgctggtg
    14341 actatctgtc tggcttaact taggcagttg ccctgttttc tcccattggt caatactttg
    14401 ttgaccaatt tttactgacc gttctgcttg ctttagcgct ccttgaaaat ccccgttcag
    14461 ataccagaga agtccgaaag caagcacaca taagagcaca actatccaaa ccagacgcca
    14521 aaacaaacgc cacacaaaaa aaaagaaagc ccctatcaaa cgaaaaagcc agcgcatgtc
    14581 tctccacctt tctagcaaat aagaattatt ttactaaaag aagctaaaag aaatctaaat
    14641 actgactttt tcatcttaac gtcctactat ttttttgagt aactggatag ctttgtattt
    14701 taaaggcgat ggatggtaac ggaaagtacc cgctttgcgt acaatatagc cattaaaatt
    14761 ctgtttaaaa cgcaaaacac catcacttcc atcaaaaatc ccttgaatgc ctaggaaatt
    14821 gtatttaggt attccacgtt ttatgctttc caacataaca tatttttgaa gcagtgcagg
    14881 ggcatagaac ttattaaact cagtgtagga accactaaag agataagtcg tttcctgagg
    14941 catataaaca aataaactcc cagctaaaac aatatcttct tctccatatt tttcaatcaa
    15001 gtctcgcgct tctgcttttc gaacttcaaa cgtttcaaat tgactagaat attctctcag
    15061 ttgattttgt tttttctcag aatgaggatt tttactcaaa tcaagtcgca acttgtccaa
    15121 gttttcttct agtttacttt gttcaccttg caatttgctc atatagtccg aaaaatttaa
    15181 gcttgctatg agaaactccg cttgttctcc aaaagtatca taaaaatgct cataatattc
    15241 taaactttta tcactatatt ctctacgttc agaggtttct tttgttatat tcttaaaaat
    15301 cgatagttct tcacgtttta actttttcaa ccgaatgcca aaggtttcag cctttttcac
    15361 caagggttta ccctttttgc taaaactttt aagcaaactc ttttcagtta attcagttaa
    15421 atctttatag tataaccaat ctggttctcc acctgggtaa cctattgtta agccatcaaa
    15481 ttgataacct aaatcagtca aaccttgaat aatacttttt ttctcagcat ctattggatt
    15541 accttggcta tcaaaagttt gataagtttc ataaggtttt acaagcaact ctaatacacc
    15601 attttgcttg gcatattctt ttaactctgc ataaaaaact ggaagagcat cttgttgggt
    15661 ataaatcggc cccgaattga gttccatatg cagaccaccc agcatgggca agctataaac
    15721 cagagctgca acttgaattt ctccttcttg tttcaaagca agataaacaa ttcgagcccc
    15781 tcttttttct agcaaatccc ccatctggac agattgcata aaggaacgag aagaaacctg
    15841 atcagaataa gtctgaaact cttctttcgt gagtgttgtt agtgccatat acttactttc
    15901 tatgtttttt tcttaatgtt ttacggaaat caagagcaag tcttaacaga ggatagagag
    15961 gatgagtggg cattgtaaat tcacccaagt attcttcaat cgttggatta aatttttcct
    16021 taaaatgata aagtccacca ttgagagagt tttcaacacc acctaaattt tgccagacca
    16081 tacctcgctc aaaggcatag cgagccgttt cataccatgt taaaattggt gcattgtaac
    16141 gtttaaaatc atcatccata ccagcatata tattgacaga ggtagtacca aattccaaac
    16201 tcaaagtagc cgctaaagga actctcgctt gacctacatc tatatattcc tgcaagaagg
    16261 tcaattcctc taacaaacgt tctttttcct tcttctgcgc ttctactttt gaagttcgag
    16321 tcgactcagt aaaggtctct tccaaggctc tatttttcgc taactgttct tctaactctt
    16381 gcgaacgttt agaaacatcc aaggtggcca aggtgatata ggccttgtcc ttaaaattat
    16441 ctaacaattt tttataatag gcttcattcc tcaaatgaat ctcttttcgc ttctcagttt
    16501 ttttcatcaa ctccgaaaat gaatctaata gttccagtcc accatattga atctcaagcc
    16561 ctttgtttcg tgctgttcga atagcctgtt ttgttgactt ggaaagttta tcttcttcaa
    16621 aattttcctt gtatattttc gcctgaatac gaggttgaat ggtgtctccc atttcctccg
    16681 tttttcctga ccaccttact cccatttgtt gcaaactatc aataatagcc agattttcag
    16741 gaaattctgt cttttcctga ttgattaaac tttgagatag gcaaatactt gggtcaaaag
    16801 tcacaaaaac cgctctctta ctgcgagcat aggacttaat agactgaatg gcaaaattca
    16861 agagttcttt atccccataa tccaatatag gtcctcttgg gatataaaac attttatagc
    16921 ctagcggaag agttctaatc aaaatactag ctgtcgccag taatttttct tccctgtaaa
    16981 caccaaactt ctcatgttgc caattagact taacttcctc ccaagcacta ctttgtaata
    17041 cattggctaa ttcatgttct ttgacaaact gatcatattc taatgtggga atgccaattt
    17101 gataacggta catttcctac tctctttcct ccagtatttt attactattc tactactttc
    17161 gtttgaaaag tctagtgaaa acatatttta ttttagttct actcccccta tgctgaagtt
    17221 caaactaagt cagtctctct tctaatttga aatcaattgg tagagttgtt aaaaaaactt
    17281 ccaattgttt ttcccagtag taccactcgt gagttccagc gctatggcta taggtcacat
    17341 caaaacctag ttttttgaga tttttcactg cgagattatt ggcttcgtac aagaaatcct
    17401 gttcgccaca ccacgcccaa agtttggtct ttttatccga ttttttagcc agactttcaa
    17461 gagaataggg actagttgtc cagtctctaa tctctccaaa aacacctctc cagtaggctg
    17521 gacttcccag attttgactt tcaggagaaa agttttgaaa gctgagggca cctgaaaaac
    17581 tagctgcatg agaaaaacga tttgtcgcaa gagccagttt gaaacagccg tagcctccca
    17641 tagaaagacc agctataaag gtcttttcac gcttgctagt catattaggg aagaagcgtt
    17701 tcagaacctg tggcaattcc tctgctagag ccgtgtagta gtcaaaacca tactgggtat
    17761 cggtgtacca accattgctg gtattgggca taacaacgat gagattagtt cctcgaagca
    17821 agcgttctac attggtccgc ttaagccaac tattatgatt tccagacatc ccgtgcaaaa
    17881 ggtacaagac gggaatatct tcacactctg gttcttccac tcgattggca tcagggtaga
    17941 ggacattcat cccccactcc atatccaata cttgtgagta atactcgatt ttcattactg
    18001 ccatgatttt ctccttctat ttttctataa gaaaaagccg aaactcgact ttctctctgt
    18061 ttatttcaac gattattttg cttccatgac tactggtaaa atagctggac gacgcttggt
    18121 ttggtcaaag agatacttgg tcaaattatc tcgaaccttc cctttaagat ctgcccagtc
    18181 aaagtcatct ccttgaagat aatcttctac cgtttggtta atcaattctg aactttcacg
    18241 gagaatatcg cgactcttct tgagataaac aaatccacgc gtgtgaacac gagctctagc
    18301 cacaattttc ttctcacgac ggttgactgt aatagccacg atgaaaattc catcctctga
    18361 caagacctta cggtcacgaa gaacaacatt tccaacatca ccaatggcat tcccatcaat
    18421 taagatatct cctgctgaaa ccgatccagc tggaacaaag tctccattct cgtaagccat
    18481 ggtcgtcccc tttttaggaa tgaagatgcg ttctggcaac atcccaactg ccatggcagc
    18541 cttagcgtga gcatccaact cacgatactc cccttggaca gggaagaggt acttaggttg
    18601 caaaagattg atcatcagct gcaaatcacg cacatttccg tgccctgata catgtaaact
    18661 ttgggtaatc aatttgacaa ccccacctgc ctgataaatc atattttcta cacgcgcaac
    18721 aaaggcttct ttagcaatag acggagccgt agcaatatag accaggtccc catccttgat
    18781 ttctacataa cgatggcgac caatcgacat cttacgaagt ccattgatag gttcacccat
    18841 acgacctgtc tcaagaataa tcaactcatg gtcttcaaag cgagacatat ctttaggctt
    18901 aatcaagaga atttcgttgg ctaaagacaa cttcttaaga cgaatcgctg tgcggacgat
    18961 attttcaata tcaaatcctg tcaagacgat acgtcgacct gttttatccg cagcgtcaaa
    19021 aatctgctga atacgagaaa gattactgga aacagctgca acgatgatac gaccttccca
    19081 gtcagcaata gtttgggtaa tttcatccct aacttcactt tcactagcca cctgaatatt
    19141 gctgtctgca ttggccgaat cactgaggag agccaggacg ccgtcacgac caatctctgc
    19201 caaacgagca aaatcagttg cataagattc actagccgtt tggtcaaatt tgaagtcacc
    19261 tgtataaacg atgcttcctt ccgatgtctt caagacaatt cccagactct ctggaacgga
    19321 gtaagtcgta gggaagaagg aaaccactgt cccaccaaaa tcaatctccg tattctcatc
    19381 aatgacatgg aaatcattaa atttcttaac ggcatcattt cctttgacaa agagctttgc
    19441 caactcaatg gtcaactcag acccaaatac aggaacttta gcctctgcca agagataagg
    19501 tagagcacca atcgcatccg catgcccgtg ggtcaagaaa accccagcaa tacggtcgct
    19561 attttcaaaa aggtaatcca tgtttggaat caccacatcg acccctaatt gttcattttc
    19621 aggatatttt aaccctacat tcaaaacaaa aatggactct ccaatttcag caatgtacat
    19681 attttttcca ttctcacgca caccaccaag tgttgttaaa ctgatattac tcatttttcc
    19741 tccaaaatct tagtttatct tgattatagg gcaactctac cctcttattc taaaagttct
    19801 aatactttca gccgaatctt ttcctaccat tataccattt ttttgatgat tttcaaaatg
    19861 aaggtctgtt ttcaaaaaag agttgatttc ccaactcttt cctaacttct actctttttc
    19921 catcaaaaaa tcataaattt cttgcaccgt acccagcgcc atcatttctt ggtctttaac
    19981 ggtttgtttg agattttggt aaatttgact ttctcctatc tcccgctttg gcgcctcttt
    20041 attgactgtc atcacaccat cttttatcgt cacaaagcct agttcttcaa atacttgaat
    20101 catcttgacc agcaagattt gttgaatatt aagatatgca gccaaatctt tcagcttgta
    20161 gcgaatatca aactctggga actggtaaat agtcttgtac aatttggcaa actgatctct
    20221 agtcccataa cctgtcagat aataggcctt gtcaatatca tttttgaaat agacagcaga
    20281 gaaatgctgt tcctgaaaaa tggtcttcag ctgagtaata tcctctggaa tgttttttac
    20341 gacaatagct tcactagccg caagatttgg cagttctcca ggaaaatcca agactggaac
    20401 accttctggc aagactgcat tttttccacg aatgttaaaa agttgaacac cttccactcg
    20461 cgcatccacc atcatcaact ggagggcagt ttggccattc cattggttga cagacaattt
    20521 aaccgctaac tctagattct tggtttgaga aaactctgtc gcccatctgc cttgaccaaa
    20581 ggctaccact tcaaaactcg cctcaccctt ggaaattttc agctttagat gggcattacc
    20641 tgcccccata gtacgagcac tttcgacctg aaaattcttg atataaaaaa taggtttctg
    20701 attatccatt ccaaaaggag ctaaacgttc aaaacttttg accgtttcca agctaagtgc
    20761 ctccaaatcc aactcttcat ctaggtttaa cttattcttg ccaccagcat ctgcaccttt
    20821 ttcacgaaca taatcttcca aaacctgaga taaatctgag agttgctcaa cttccagcgt
    20881 catacccgct gcacctgcat gacctccaaa ggcgataaag aggtctcgat ggggatccag
    20941 agcttcaaaa atatcgaccg cttccacact acgagcactg cccttggcac gaccgtcttc
    21001 tatattaaga acaatgactg tctgtcccaa ttcttccaat aaacgaccag ccacgattcc
    21061 tagaacccca ggattccagc cttccttggc caagacctga accttcttct caggatccac
    21121 catggtcttg gcttcttcat agatagactg aacgatttcc ttgcgctctt cgtttttctg
    21181 gtgaatcata aaggcaatct catgcgcttc ctcatcatca aatccagtca acaaatcaat
    21241 ggcaggattg ggatcatcca agcgacccaa ggcattcaaa cgaggagcaa tctggaaacc
    21301 aaccgtttct tctgttactt cgttggcagc aatcccagcc atgtccagca tttcttgcag
    21361 accaatgcgc tgggtatgac ccaacatttc cagaccatat tgaactaaga tacgattttc
    21421 atccgtcaga ctcaccatat ctgcaatagt tccaatagcg accaaatcaa gcaattccac
    21481 ttgcacttct tctaacaggg cacaagccaa cttgaaagca actccacaac cagccaaata
    21541 tttaaaagga taatccgcat ctggatgttc aggatggaca atagcataag catctggcag
    21601 ggtttcaggc atggaatgat ggtctgtcac aatgacatct actcccatag actgagccaa
    21661 tgcaatagcc tcatgaccag caaccccatt gtccaccgtc acaatcaagg aaattccttc
    21721 ttgctcgata aagtatttat aaacactagc attagggcca tagccatcgg taaaacgatt
    21781 tggcaggtaa actcggcact cagcaccaag ttgttccaaa ctttccttca caatagaagc
    21841 cgaagtcatg ccatccgcat catagtctcc ataaacgaga atattttccc cttcttcaat
    21901 agcctgacga atccgctcca ctgccttgtc catatcatgg agcagataag catcatgtaa
    21961 gtcctccaag gaaggttcta aaaacttctt cagactttct tggtcctgaa tccctctctc
    22021 aaacaataac cgagccacct caggacccag tccagccttc ttggctatct ttgtaaaatc
    22081 cgcatcttct acctgcaggg caaactgcca ttcataagta ggtgttatca aaaagacatc
    22141 cactctttct aagaattcta ttgcttcatt ataacatgat ttagacgttt tctctatcca
    22201 gattgctttt ttataacatt ttatagtata ttgaaactag aatagtacac ctctacttct
    22261 aaaatattgt tagaaatcga tttgactgtc ccgatcgatt tgtcctgttc ttatttcact
    22321 atagtgaatt ttttcagata tggtttgaca agaacattct tttattgtag aatcgtgtta
    22381 taaaatctaa cacaaaggag atctctcatg gctttagtag aatttaaaaa cgtcgaaaaa
    22441 tattacggag actaccacgc actccgcaac atcaatctcc gttttgaaaa aggacaagtt
    22501 gttgtcctgc ttggaccttc tggctctggg aagtccactc ttatccgtac gatcaatggt
    22561 ttagagactg ttgacaaagg aagtctccta gtcaatgggc accaagttgc tggtgccagc
    22621 cagaaagatt tggtacctct tcgcaaggaa gtcggcatgg tttttcaaca ttttaacatt
    22681 tatccacaca aaactgtgtt agaaaacgta acacttgcac ccattaaagt tctaggaatt
    22741 gataaaaaag aagctgaaaa aaccgcccaa aaatatctgg aatttgtaaa tatgtgggac
    22801 aagaaagatt cctatcccgc catgctatct ggtggacaaa aacagcggat cgccatcgct
    22861 cgtggtcttg ctatgcatcc ggaactcctc ctctttgatg aaccaacatc tgctcttgat
    22921 cctgagacta tcggagatgt tctagcagtt atgcagaaac tggcgcatga tgggatgaac
    22981 atgatcatcg ttacccacga aatgggcttt gctcgagagg ttgcggaccg cattatcttt
    23041 atggccgacg gagaagtttt agtagatacg acagatgtcg ataacttttt tgacaatcca
    23101 agcaaacctc gtgcccaaca attcctcagc aaaattatca accacgaaag tgacaaagtc
    23161 aaataaggag gcgcctatga aaaaaaaatt ctttttatca gcattattga ttagcctttt
    23221 cggccttgct gctgccaaac cagtccaggc tgatactagt atcgcagaca ttcaaaaaag
    23281 aggcgaactg gttgtcggtg tcaaacaaga cgttcccaat tttggttaca aagatcccaa
    23341 gaccggtact tattctggta tcgaaaccga cttggccaag atggtagctg atgaactcaa
    23401 ggtcaagatt cgctatgtgc cggttacagc acaaacccgc ggcccccttc tagacaatga
    23461 acaggtcgat atggatatcg cgacctttac catcacggac gaacgcaaaa aactctacaa
    23521 ctttaccagt ccctactaca cagacgcttc tggatttttg gtcaataaat ctgccaaaat
    23581 caaaaagatt gaggacctaa acggcaaaac catcggagtc gcccaaggtt ctatcaccca
    23641 acgcctgatt actgaactgg gtaaaaagaa aggtctgaag tttaaattcg tcgaacttgg
    23701 ttcctaccca gaattgatta cttccctgca cgctcatcgt atcgatgcct tttccgttga
    23761 ccactctatt ctatctggct acactagtaa acggacagca ctactagatg atagtttcaa
    23821 gccatctgac tacagtattg ttaccaagaa atcaaataca gagctcaacg actatcttga
    23881 taacttggtt actaaatgga gcaaggatgg tagtttgcag aaactttatg accgttacaa
    23941 gctcaaacca tctagtcata ccgcagatta aggaggatac cctatgacag atttatcatc
    24001 ttggacagcc tattttcagg attttggaca atttttcaat ggtttcctct ttactcttgc
    24061 cctagcgatt ggatccttta tcctcgccat ggtcttagga atcttctttg gtgccatgtc
    24121 aaccagtaaa cgtccaattt tacgcatttt agctcgcatc ttcgtggaat tttaccaaaa
    24181 cactcccctc ttggtgcagt ttgttattgt tttttatggc ctacctctta tcagtgacca
    24241 catcatcatg attccaattt attggacagc cgttctctgc gtgggactct atcacggcgc
    24301 ttatatcgct gaggttattc gttcagggat tcagtctatt cctagtggtc agatggaggc
    24361 cgccttgtcg caaggtttta cctatatcag tgccatgcgc ttgattatct tgcctcaggc
    24421 cttccgcatc attctccctc cattgaccaa ccaaattgtt aacctcatca agaacacatc
    24481 tactgtagct atcatctctg gagtagactt gatgtttgtg actaagtctt ggtcggctct
    24541 caacggaaac tatatcccag cctttttagg tgctgctctt ctctactttg ccctatgctt
    24601 ccctgttgcc cagtttggtc gcaagatgga gcaagccaat aaaaaagcct attcacttta
    24661 ggaggttact atggaatcta ttttagaaat tttaacccca gataacctag tctttatctt
    24721 taaaggattt ggcttgaccc tctatatttc tctgattgcc atcatcctct ctactatcat
    24781 cggtaccgtg cttgccgtca tgcgaaatgg aaaaaatcct atcttacgca ttatttccag
    24841 tatttatatc gagtttgtgc gcaacgttcc caaccttctc tggattttta ctatcttttt
    24901 ggtgttcaaa atgaaatcca caccagcagg tattacagcc tttactctct ttacatcagc
    24961 agccttggct gagattattc gaggcggtct caatgccgta gacaagggac aatacgaagc
    25021 aggaatgtca caaggcttca cctcagccca aatcctctac tacatcattc tcccacaagc
    25081 catccgcaaa atgctaccag ccatcatttc tcagtttgtt accgtgatta aggataccag
    25141 tctcctctac tctgttatcg ccctacaaga actctttgga gccagccaaa ttctcatggg
    25201 ccgttatttc gaaccagagc aggtcttcag tctttacatc ctgattgccc tcatctactt
    25261 cagctttaac ctagcgattt ctagcctgtc tcatatgcta gccaaacgtt ggcaacaagc
    25321 tgcagaataa tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtactc
    25381 aagtacagcc tgcggctagc ttcctagttt gctctttgat tttcattgag tataaacagc
    25441 cactctccag ttcaattgga gagttttttg atgagagtag atatttatag acctcaattt
    25501 gacaccatac attttctatg ataaaatgta acaaattcat ttacaacaaa tcgaaatcta
    25561 aatgaaatgg aattttctat gaaatcagtc aaaggactac tctttatcat agctagtttt
    25621 atcttgactc ttttgacttg gatgaacact tctccccaat tcatgattcc aggactagct
    25681 ttaacaagcc tatctctgac ttttatccta gccactcgtc tcccactact agaaagctgg
    25741 tttcacggtt tggagaaggt ctacaccgtc cacaaattca cagcctttct ttcaatcatc
    25801 ctactaatct ttcataactt tagtatgggc ggtttgtggg gctctcgctt agctgctcag
    25861 tttggcaatc ttgccatcta tatctttgcc agcatcatcc ttgtcgccta tttaggcaaa
    25921 tacatccaat acgaagcttg gcgatggatt caccgcctgg tttacctagc ctatatttta
    25981 ggactctttc acatctacat gataatgggc aatcgtctcc ttacatttaa tcttctaagt
    26041 tttcttgttg gtagctatgc ccttttaggc ttactagctg gtttttatat catttttcta
    26101 tatcaaaaga tttccttccc ctatctaggg aaaattaccc atctcaaacg cttaaatcac
    26161 gatactagag aaattcaaat ccatcttagc agacctttca actatcaatc aggacaattt
    26221 gcctttctaa agattttcca agaaggcttt gaaagtgctc cgcatccctt ttctatctca
    26281 ggaggtcatg gtcaaactct ttactttact gttaaaaatt caggcgacca taccaagaat
    26341 atctatgata atcttcaagc cggcagcaaa gtaaccctag acagagctta cggacacatg
    26401 atcatagaag aaggacgaga aaatcaggtt tggattgctg gaggtattgg gatcaccccc
    26461 ttcatctctt acatccgtga acatcctatt ttagataaac aggttcactt ctactatagc
    26521 ttccgtggag atgaaaatgc agtctaccta gatttactcc gtaactatgc tcagaaaaat
    26581 cctaattttg aactccatct aatcgacagt acgaaagacg gctatcttaa ttttgaacaa
    26641 aaaaaagtgc ccgaacatgc aaccgtctat atgtgtggtc ctatttctat gatgaaggca
    26701 cttgccaaac agattaagaa acaaaatcca aaaacagagc ttatttacga aggattcaag
    26761 ttcaaataag taatcatact ctcctgggat ttcccaggag agttttttat agccaaatga
    26821 aataagacct gaacgatgga attttggcat tcaaatcaat tgataagaat attttatagt
    26881 ggagtgaaat aggatacgaa caaattgatt aggaaaatca aatgaattta tagaaatctt
    26941 ttagcagtta tgttgtccta ttctagtttc aaaacgctat agaagcagca ttgtgctagt
    27001 ctagattcag tttactatac taaaacgagt agcttgaaat caaaaaaccc accctcacag
    27061 gcaggtttta tctgtattat tcagctagat tatgctttac cttctgaacc gaatacgtcg
    27121 atacgttctt caactgatgc ttggatagct tttacaccgt cagccaagaa tttacgtggg
    27181 tcgaagagtt ttttcttgtc gtattctgct tcgtttgctt cgtagtcacg agcaaattta
    27241 cgagttgcgt tagcgaatgc gatttggcat tctgtgttaa cgttaacttt ggcaacacca
    27301 agtttgatag ctgcttggat ttgctcatca ggaatacctg atccaccgtg caatacgatt
    27361 gggaatcctg gaagagcttc tgtcaatttt tgcaagtggt caaggtcaag accttcccag
    27421 tttactgggt aaggaccgtg gatgttaccg ataccagctg ccaagaagtc gataccagtt
    27481 tcaaccattg ctttagcgtc ttcgattgga gccaattcac ctttaccgat gattccgtct
    27541 tcttcaccac cgatagtacc aacttcagct tctactgaga tacctttagc gtgtgctttt
    27601 tcaacaactt ctttagccaa tttaagattt tcttcaactg gaaggtgtga accgtcaaac
    27661 atgattgaag tataaccaac ttcgatacac tcaagtgcat cttcgtagtg accgtggtca
    27721 aggtggatag ctactggtac agtgataccc attgattcaa caaggttagc gatcaagttg
    27781 cgagcaactt tgtaaccacc catgtattta gcagcaccca ttgaagtttg gatcaaaact
    27841 ggagcttttt tagcttctgc tgcgcgcaag atagcttgag tccactcaag gttgtttgtg
    27901 ttaaatccac caactgcata accgttgtca cgggctgctt ggacaaattt ttctgctgaa
    27961 acgattgcca ttttatcagg cctcctgtat atttttatgg gtcatcccat ttacattgtt
    28021 cattttatca ctttttgcca aaaaaatcta gtttttcccg cagtttcaat tgattttctt
    28081 ctaactccac ctatgtaaac cctttctctc cctagtcttg gacgactttt ggaaaatcta
    28141 taaagaaggt taaacgattc tcctccatct cgaaacgata agctaatttt tcatgttcta
    28201 atagactctt aaccacaaag agccccatac cagacccctt gaccttgcga ctagcattgt
    28261 cagaaaaaga ctgggctagt ttttcttgtt cctctgagct acagctattt tcgataaaaa
    28321 gttctccttc tctttctcca attcgaacta agccacctgg aacagagtgc ttaatggcat
    28381 tgctgatgag attagaaaga atcaacttca taactgatgg gtttagataa gcctgctgat
    28441 gggtcaaact attgtctatc tggagctctc tttccttggc tagcaaggca taatatttga
    28501 ccagattttg cgtcatctgg aggaggtcaa ttgtttccct atcatctcgc aattcctgca
    28561 cagaagagag ggaaagtatc tgcagaacat ggtgattgag ttcatccaca atccccaagg
    28621 caactcccag atactggtct ctatccttat aacgaccgat attctctctc atattttcga
    28681 ttaggatttt caaactagcc agcggtgttt tcaattcatg agaagctcct cgtaggaatt
    28741 cgaccttcat cttctccagc tggagaatgg cttcattctt ttcatgcaag tccgcaataa
    28801 cagtcaagag atgctggtag aggctattga tttgttcctt gagattacct atctcatcct
    28861 tagaatccac gcgcaatcgc acttgggaat ccaggtccat catccgacgg gtcacccgct
    28921 tgatttccaa aatcggtgca acaatagtcc gagcgtagat gtaggccacc aagagggaaa
    28981 tcagaaagga ggccagcaag gtatagggaa gaaactggag actgatttgc tccgcttcct
    29041 tttgtaaatc catggaagct agaaactgga gaatcatagt accaccgtct tgcgttttca
    29101 cctcgcgctc ctcaataaag agagaggttg tctggcggtc tgtgtccaga ggaagactgt
    29161 ccttgacttc taacttgtcc tcggtcatct cacctttgac ggtccccttg atatcactag
    29221 tctgggaata caagtctaac acttgctcga tactctgcct atctttccct tctagggact
    29281 gggcaatggc tgttgccttt tgaccaatag tttcctgacg atgactcaga taagtcgaag
    29341 gaaaaagaaa ataaatagct aaatgaaggc agataaccag aacactaaat atcgagaagg
    29401 tatagataaa tatctttgca aataaacctg ttcgtttcat tttcgctcca atttataacc
    29461 aacattgcgc acagtgagga tacaatccaa gtctagcttt ttccgcaatt ccttgatata
    29521 aacatcaata acacggtcaa agggaacctc atctgtcgct ttccagacgg aatcgataat
    29581 ctgagaccga gtcaaggccc ggccttcatt tttcactaga tagtccagaa tttccaactc
    29641 tttggcattg ataggcactt cttgacctgc gaggcttgca ctgtagcttt caaagtccac
    29701 cttggtatcc ttgtaagaaa agattcgtcc tgtatcgtag tagcgcttga aaatcgcgtc
    29761 caccctcact tttaagaggg agagggagaa aggtttttcc agatagccat ctgccaaaga
    29821 ggcaaaggca ctcatcttgt attcctcatc ctgaaaagct gtcaacatca agacaggaac
    29881 ctgactggtt ttacgaatct cagctagaac ttctaagccg ttaagcttag gcatctggat
    29941 atccagtaaa accagggcca cctcatagct agaaaattgc tccagagctt cctgaccgtc
    30001 cgctgcctca atagtttcat agccacaatc cgtcaaataa tcactgaccc cctcacggat
    30061 catctcttca tcttctacaa ttaaaatttt catactttaa ctgctctcta ttttttattt
    30121 ttcttagaat aaatacctac tctattttct attatagtct cttgctggcc ttttgtctgt
    30181 aagtaactga tcactagata aaacgttgtg aaattccttt ctcataaatt ccatagcttt
    30241 agtatattat atttaagcaa taaagtacaa agaaaacaac tgaaagcaat gattttcacc
    30301 actgctttcg gatttatttt gaattgttaa atagccattc ctatccacta ttcttgaata
    30361 gaaacacaag atgcaatctt tattccagac tcattttttc aaatttattc accatccagc
    30421 aagagctctt ttggttgttt tctaaggaga ttgcttgaag caagcgccat aacgagaacc
    30481 actagaacca aggcaaggac aaaaatgatg ataaagtctg atgtctgaat agaaatgtct
    30541 aggctcgaca aggtcttgct aaagccatct acttctgcac caccaccaag gttagaggct
    30601 tgagccgcct tactagcctg tttggcaaca cctgaagtca cattggcaag gacagtgttt
    30661 ccaattgcac gggcagtgta attagctagg aagtaagcag aaactagagc agggatagca
    30721 atcaagatag attcggtgat gaattgaccc aagatacttg cctgcttgag gccgatagag
    30781 aggagaattc ccacttcctt gcgacgggcg ttgatccaaa ggctgagcaa gagggcaagg
    30841 aggagaactg agaagctcaa gctaccccag aagaggaggt tggccatctt gtacatacca
    30901 gagatagatt gctcaagagc tgggtagtta gaggagctct tgacgagtgt gtagctcttc
    30961 cagttgatac cactgatgcc attcaactct ttcataacat catccaagtt cttgtctgct
    31021 gttacaaaga aggttgcgtc cccataaatg gctgtgtctt ctgtgtatcc ataaagtttt
    31081 gcagcagtgt gaatgtctgt aatagctgtg ttttcgtaaa gttcttgtga gtaggttact
    31141 gctgacttat tatgaccatc aaagagtccc ttgattgtca cttcaactgt ttccttggct
    31201 cctttttcat tatctgcatc gtagatatta gagtccagtt taaccttgtc ccctactttc
    31261 cagccgtgtt tggctgccaa gtccttgtgc aagaggattt tatccttgtc gtcgttggtt
    31321 aagtgctctc cttcgactag tttataagaa ccagagacaa acttgtcttc tttagaggag
    31381 tcattgacac ctgtaatcat caagctactt ccaaaacgtt tggcacgatc agcagtgaga
    31441 ttcttcttgg tttctggcgt ttcaatcagg tcatatccag tcaaatctcc gatagcgttg
    31501 atacgtttga cataagactc aatggccttg ttttcggtga tttttttgat gtcttcaccc
    31561 ttgatattcc cagcaccgcg aggcgttcct tggttgacgc gacgattgat ttgcatggag
    31621 aagctattgg tgatattttt aaaggtctcc tgagaagcct tggcagtagc tcccttgatt
    31681 gacaagccga ccaaactcaa gctcgccatg aggagaataa tcaggaagat gacaatcgat
    31741 ttgaaaaact tccttgtaac ataggcaaat gcgttgtgta acatagattc cctttctaga
    31801 ttttgtttta atcattctat taaagtaagc tcaaattatt tactagtatt gcgcgtttca
    31861 gtcagtttct tatcctttaa ttcaagtgta atatctgacg cttgtgccac ttctttactg
    31921 tgagttacga caatcacaca tttacctgtt ttctgggcaa gtgatttgag tagttcgaca
    31981 atatctccag cagttttagg atccagattt cctgttggct catcagctag aataactgga
    32041 gcttctgaga ccaaactgcg agcaatggca acacgttgct gttgaccacc tgataactgg
    32101 agaacattcc gcttgatctg gctttcatcc aaaccaagct caagaagtgt attcttgctt
    32161 gcctttttgt tgaccaatcg gatattttcc agcggagaaa gataatctat caagttataa
    32221 ttttgaaaga ccagggaaat atggtgcatg cgatggtaag aatagccctt cttacgaata
    32281 tcctctcctt gaaaaaggat agaaccttca acaggactat ctagaccagc aagtagggac
    32341 aagagtgtgg attttcctgc tcctgactcc ccaataatac tgtaaaattt tccgggttca
    32401 aaattataat tgatctgata taggactgct tcagcagtat tcttataacg gtaggtaaca
    32461 tcttgtaatt gtaataaagt catgatttct ccttcttaac taatagatga taaaatttct
    32521 ttcggtgatt ttctaaataa gaataggaaa caaagggcta cagataagca actaagcaga
    32581 actagaaaaa cataggattc tgcaaaagat aagatgctag ttgataaact gcttgctttg
    32641 gctagtgtat cttgtaagct tgcctgatct ccacttgcta gtagagtttg gagtaggtaa
    32701 gttgtgattc cgtttcctgc aacaaatgct ggaagcaaag ctccaagaga taccaaaact
    32761 acctctaaac agaattgtag gaaaatcgag ctcttgcctt ttccaagtgc aagtaaaatc
    32821 cccacttcat agacccgttc tctcaaccag agagacaaaa ccagaattaa ggctccagat
    32881 cctgctatca acatcccata aaggaagatg gtcaggaagg tttggaaagt tgcaactgag
    32941 tctttgattt gttcaaaagc cttgttttcc ttttcgactt ggtagccttg attttccaag
    33001 gccaagtttt ctacctgctt catgagtccg tccatttcct taggattttc tacatagaag
    33061 cgtgctgcac tgacttgagc ttcactattg cccaaaaggg tttggctact ttcatagtct
    33121 gtaaagactt gattttcact gaagtcagaa gacaagcctg tgaatttctc ttgtttttta
    33181 ccagaaaaga tgccgataat ctcaaactct actgtttgtc cttttccaga ttcagactga
    33241 ccagcatcca agccaatctt gtcatgaagc gaaagaccgt tcttcttagc caattcttcg
    33301 tggataagga ttttcttgga atcccctttt tgaaggtgtc gcccttcttt tagattgaaa
    33361 gccgaactgg taaaggttac atccttggat gaatcctcaa gagccgttaa gctaaccaag
    33421 ttattgtctg cagctgataa atcatcacgc tccacgctct gctcgccagt cactgcttcc
    33481 ttgtctttta gttttgcgac cgtctcaagt tcaggagaga cattttccag ccccttaatc
    33541 ttgcttacag atgctaggtc tgacaacttg aatgtctgac cattctctat cttcttaata
    33601 gaaaaagatg tattgagtga tttataaaga ttgctttcta ctgttttgtt ggacttcatc
    33661 agagtcaaac aggctgaaat tccggccaat aagaccaata aaatcagaaa taaaataaaa
    33721 cttctcagtc gctttctgct gacataagcc caagctcttt ggattggatt catttgtcac
    33781 ctccatattt gtaagactat tataaaaccc aaatatgaaa tgtttatgaa atacgaaaaa
    33841 aaaatatcga gtaggggata atctctagcc cctctcacac caccgtacgt gccgttcggc
    33901 atacagcggt tcaactaact tttaacgcat gtcgttcaag gtaataatcc aaacacgaaa
    33961 cctgttcacg tttttcaagg actggttttg atatagcacg tttaagtacc gacttctgag
    34021 ctactatagt agattgaaac tagaatagta catctcttct tctaaaacat tgttagaaat
    34081 cgatttggct gccctgatcg attcgtccta ctcttatttc attttactat aattgatagt
    34141 ggtcgctcca gccagatacc ttatctgcta tccatttagg aacccctaac ttaagcaatc
    34201 cccataatcg tctcgatttc ttcttccatt gcttccagat aatcactcgt aggcgagtac
    34261 gcaagcgctc atctatgcta gtgactatac ttttcatatt tataattcat tcctttcgtt
    34321 tcactcaagg cacaacacag aatgaaaaag tgttgtgctc tttattttgt tttataataa
    34381 tagtgagaaa gcctatcact actacaaatc acggggaggt gaataagtga gtggtacagc
    34441 cactacctcg catattttgt cacatcattt aacggtacat aataagttgt accatctgaa
    34501 taagttgcta caatatcatt tgcatgctct ccttcacctt tagcaaaggt tggagctcct
    34561 gctggataat ttttatttgc ctctttcaat ttttcaataa tggcattttt ttctgtatct
    34621 tttatattat caggattttt cactaagatt ttgtctggat atgtcggttt agcagaaaca
    34681 atttttactg ttatttcttt tttattcgaa gcacttgtcc agtttccagc attatcttta
    34741 gcatttaatt ttacagtaat tcctgaacta ggaacttcag tagcaggttg attatcaaca
    34801 ttattcaact ttaatttcaa aagagctgtt gcatcagacg ttttatcaat cgttgtatat
    34861 aatgatgaat tgttattata accagtttct tcatatttag ctgtttgtga gctacttgaa
    34921 acagaactga aattatgccc actacctccc tgattatctt caatgcttac gtctaaatga
    34981 acttccccac tattatttgg cttagcaaca actgttatag taaaataaca taaaatttgc
    35041 ataaatagat tagggaaatc aaagcagctt ctaggaatgt tttagcagtc acagtgtact
    35101 ttcccagcat caagccacta taactctgca cataaaaatg gagaagatgg caatcctctt
    35161 ctccaaatat taacttcttt acaaaccaac tatagttgac aaagaaccta aaatcaattg
    35221 ataacacaag gtcaggtcgg tcaactcttt caactgaagc cctgtcaact cttcccattt
    35281 atcaatcttg tattggagag aattgcggtg cagatagagt tgctgggctg ttttagtgag
    35341 aacagcacta ttttcccaaa gagagagaat gatttcctga atctgatctt gatccaaaat
    35401 catctggtgt agacattctt taattggctt caagtccacg agtctttctc ccatactcca
    35461 aagatagagc tgagaaaaag tatgaacacc ttggtgaccc tgacgccacc atgtcttgaa
    35521 caaatcccgc tcagctttga ttaagtctga tagggcttga tgtcctgtct gagaccaaac
    35581 ctgacccaac atgatagaaa gacgaagtcc aaagtcatac tcaaccgctt caatcgtatc
    35641 acttaaaata tctcttacag aagtgtattt gtcttgttga agcacgaaaa cataatcctg
    35701 agctccgacc tgtagcactg tctgacaatt cggaaaaaga gtccgcatca tatctagcca
    35761 agaagccaga ttttcctgct gaaaataaga aagatggcaa taaaccaact gaatcttttt
    35821 aaaaacttgc ggtgcctgtc ccttgccttc aaccagatag gaataccaag ggtttagcga
    35881 acgagcctgc tcctgctggg tcaaaagggc aaccaactgc ttttcacgct cgctgagccc
    35941 agcttcctcc agcaaaatcc actgctgaga agctaaaggg agcgtgagat agccctcttt
    36001 ctctactggt tggtctgaaa tccgagcctc aggaaaccag tcttgtagtt ctttttccct
    36061 catgttctag ccctccactt tttggatgca ccatgaaacc aaactctcaa gacgttccag
    36121 attctcagtc atatggagat agcccataac cgcttcaaat cccgtggaca tacgataagt
    36181 cacgacatct gcatttttag cctttgtgtg gctattggta ttgcggccac gtttgtagat
    36241 ttcttcttct ttttccgtta ggacctgctc ctccaacatg agagcaatca ggcgagcctg
    36301 agccttggct gacacatact tggttgcttc ttgatggagt ttattgggtt tggtcatacc
    36361 tttgaggatg aggtgacggc gaatatacat agaatacacc gcatccccct caaaggctag
    36421 cgcaatcccg ttaatgagat tgacatcaat cacgtgtcca cctcactcca tccttggtat
    36481 caaggagctt aattccttga gtaaccaatt ggtcacggat ttggtctgct gtcgcaaagt
    36541 cacgattggc acgcgcctct tggcgttttt gaatcaagtc ttcaatctct gcatccaaaa
    36601 cttcctcaac aaagacaatt ccaaaaattt ctaacatatc tgcaagagct tgcttgacac
    36661 ttgcatcata gttccctgag ttgatccatt tggccatttc aaagacaact gtgataccgt
    36721 tggcagcatt aaaatcttca tccatagctg ctacaaactt atctttaaag ttttgtaact
    36781 cttgggcatc cacgtttcct gtaaatggtt gttcgtaagt attcttcaga tacttgagat
    36841 tggtctcggc atcgcgaact gccttttccg tgaagttgat aggcttacgg tagtgctggg
    36901 tcgcaaagaa gaaacgaagt acttgcccat caagagtttt aagggcatcg tgtaccgtaa
    36961 tgaagttacc caaggactta gacattttga cattgtcgat attgacaaag ccattgtgca
    37021 tccagtagtt agcaaaagcc ttgcctgttt tagcttcaga ttgggcaatt tcattggtgt
    37081 ggtgtggaaa ctctaggtca gctccaccac cgtggatatc aatggtatca cctaaaatct
    37141 ctgtcgacat gactgaacac tcaatatgcc aacccggacg tccaggtccc caaggactat
    37201 cccaagaaat ctcacctggt ttggaagatt tccatagagc aaagtctaca ggattttcct
    37261 tacgagccgt ttcttcatcg gtacgacctg aagcacctag ctccaaatct tccaaggttt
    37321 tattagccaa tttagcatag ttgtgggatt tttctacacg gaaatagaca tccccttgac
    37381 tctcataggc aaagcctttc tcgatcaagt cttccacaaa acggatgatg tctgccataa
    37441 actccactac acgcggatgg cgagtcgcag gtttcacgcc caaggccgtc acatcctcac
    37501 gaaaggcagc gatgtactta tccgcaacct cctgaggcgt gatgccttct tccctggcac
    37561 ggttgataat cttatcatcc acatctgtaa aattggaaat ataggcaacc ttatacccac
    37621 ggtactcaaa atagcgacga atcgtatcaa aagctaccgt cgaacgggcg tttcccacgt
    37681 ggatatagtt gtacaccgtt ggcccacaaa catacatctt gatcttgccg tcctcaatcg
    37741 ggacaaattc tcgcaaatca cgagacatgg tgtcatagat tttaatcata aatcataatc
    37801 aggaaagcta aaatccaaga actgttagtt tcatcactaa actaagagct caagtaaatt
    37861 tcagtccgaa tgtctctaca cttcggaatc ccttgctcct ttctcattca gataaaccac
    37921 ctgtgtctgt ttgacaaagc caattttttc atacaaacgt ttggcaccta cattgctatc
    37981 ttccactgca atctgaaatt ccttatcatt ttgctcaatt agttggttga caagggattt
    38041 tgctaagtag cttccatagc ctttcccacg ttcaggttct gatattgcta aaccgtagaa
    38101 gtaattcgta tcgctcgata aatcaaccgt gcaagtccca ataacctgac cagcttttaa
    38161 taaaatatat agtcggcttt ctggatcttt cagagcttca gcgacatatc tatccacaac
    38221 ttctctcgat tcatgttcct ctgaaaatgc ctgaaatttt aattgactaa tttgatcctg
    38281 atacgaacta tctgctaaca aaatttcaag atgggaaaca tttgctaacg gataaggtct
    38341 tctatcctta cctaaccaag tttctgtctc ttcatcctcg attagtcccc agttactggc
    38401 aaagtcagga tgattctcta aaaaaatacg ttctgtctga aaagtgactg accgaatagg
    38461 gaaagaagct gtttctctct caaaactagt aaacaatgca cgcgcaatcc cctgacggcg
    38521 atgacctgga tgaaccagta tcgtcacttc tacatcttgg tcatctgcat agacagttaa
    38581 taaaccaaca agtttgcctt tttcataata aaggaaaaag gcgggcatgt ttgggtcaaa
    38641 attaagcatg ttagagagat agggatcgcg ataggtaccg tcatagtttt ggcaacagtt
    38701 aattactttt ttcgccccag atagctcctc ttggcttaac ttgtttcttg cttgaatcat
    38761 ataggtatcc tctacaaacc agacgatctg tgactggcat ctttagcctg ctcgagttta
    38821 ttgacataat actctcgttt ttcttcgact tcgtgaataa caggctcatc tttcttacca
    38881 tgaagacgga caatcttggc cggaataccg acaaccgtca catcactagg tacatctgct
    38941 acgacaactg ctgcagcacc gaccttggca ttttcaccaa tttccacagg tccgataact
    39001 tgggcatggg ctgatatgag ggctcccttt cgtacagtcg gatggcgttt gccacagtct
    39061 ttccctgttc ccccaagagt cactccgtga tagagaagaa cgcctttttc aacaatcgct
    39121 gtctctccaa tcaccagacc agaaccatgg tcaataaaga cacctgaatc aatctgggct
    39181 cctggatgaa tctcaatctg agtccaaaag cgccaaaact gactgtgcat acgagctaag
    39241 agtttgaagc tgtgcttcca gagaaaatgc gagagacggt gggccgccaa ggccttgaca
    39301 cctggataag tcagcaaaac ctccaaagtg gtgcgggccg ctggatcatt ttcttttacg
    39361 atatcaatgg tttcgcgcca ccaccccata catttctcct tttcttattc tgaatctttt
    39421 ggtgtttctg taaattcttt cttaggtttg tgatccttgt gatgacgtgg gcggtgaggg
    39481 cgctcagact tttcaccttt ttcatcatgc tcaggttttg gcggacgagg tagaagagcc
    39541 ttcatagagg catcgatacg gcctttttca tcaatcttga taaccttaac atcaacttca
    39601 tccccgattt ctaccaaatc ctctacacga ttggtacgag tccaagccat ctcagagata
    39661 tgaacaaggg catctgtctt atcaaagagg ttaacaaagg caccaaattt ctcgatacga
    39721 acgactttag cacggtaaac ttcatccact ttggcttcac gaaccaaacc agcaataatt
    39781 tctttggcac ggttaatagc atcttggtca ctagagtaga tagacacatt tccttcttcg
    39841 tctatatcaa tcttaacacc tgtttcagca ataatcttgt cgatggtttc tccaccctta
    39901 ccgatgacaa tcttaatctt gtccacatca atcttgatcg tatcaatttt cggagcagtt
    39961 ggagccaatt ctggacgaac ttctggaatg gttgcttcaa tgacatcaag gatttcaaaa
    40021 cgcgctttct tggcttgagc aagagcctcc gtcaagattt ctgcagtaat cccttgaatc
    40081 ttgatatcca tttgaagggc tgtaatccca tcacgagtac ctgcaacctt gaagtccata
    40141 tctccaaagt gatcttccaa accttggata tctgtcaata ctgtgtagtt atttccatct
    40201 gagataagtc ccatagcaat accagctact ggcgccttga ttggcacacc accagccata
    40261 agggcaagag ttcccgcaca gatagaagct tgagatgaag aaccgtttga ttccaaaact
    40321 tctgctacta gacggatagc gtatgggaat tcttccaagc ttggcaagac ttgagcaaga
    40381 gcacgctcac caagagcacc gtgaccgatt tcacgacgac ctggcgcacc gtaacgacct
    40441 gtttccccta cagaatattg agggaagtta tagtggtgca taaagcgttt cttgtactct
    40501 ggatccaaac catcaatgat ttgagtttct cccatcggag ccaaggtcaa gactgaaaga
    40561 gcttgagttt gcccacgagt aaagagacct gaaccatgta cacgaggaag gaagtcaaca
    40621 accgcatcca aaggacggat ttcatcgacc ttacgaccat caggacgcac cttgtcttct
    40681 gtaattaaac gtcgcacttc tgcgtgttcc atttgttcca agatttcagc cacatcacgc
    40741 ataatacggt caaattcttc gtggtccgca tatttttctt cgtaaacggc agtcacttgg
    40801 tctttcactg cttgagttgc agcttcacgg gccaatttct cttctacttg aactgccttt
    40861 tggaggtcac tgttgtaggc tgcaatgatt tcagcttgca attcagcatc cacgtgaagc
    40921 aattccactt ctgctttttc tttaccgaca gcagcaacga tttcttcttg gaaggcaatc
    40981 aattctttga cagcttcgtg ccctttaaga agcgcttcca acatgatttc ttctgacaat
    41041 tctttggcac cagactctac catgttgata gcgtgcttgg ttccagctac tgtcaattca
    41101 agaagagatt gctctgcttg ttcttgactt gggttgatga tgatttggcc atctacatat
    41161 cccacttgta ccccagcaat tggtccgtca aatggaatat ctgaaataga cagtgccaaa
    41221 gatgaaccaa acatagcagc cattggtgca gatgcatttt catcataaga aagcactgta
    41281 ttgatgactt ggacttcatt acggaaacct tccgcaaaca taggacgaat cggacggtca
    41341 atcaaacgcg ctgtcaaggt cgcatctgtt gaaggacgtc cttcacgttt cataaagcca
    41401 ccaggaaact tcccagccgc atacattttt tcttcgtagt tgacttggag tgggaagaaa
    41461 tccccagttg ccattttctt agacataacg gcagcagtca agacagttga ctcaccgtaa
    41521 cgtacgacaa cagatccatt tgcttgctta gcaacctgac cagtctctac aattaactca
    41581 cgacccgcaa aagtcgtttg aaacacttgt tttgccattt taatcccctt tggattgatg
    41641 aaattatacg ccttgcctac aaagatcaag ataccaagga cgtcaaaagc aaagtaaaaa
    41701 taggaaactg acgaagtctt cgatgaagac aagacagttt atctttttta cacagctttt
    41761 cggccgtgtt caattacaca agatattttg gacggttcgg cttgccgaac atttctgtag
    41821 aaaaatagga aggtgacgtc gcactcgacg agtgctagga agcttatctt ttttcctaag
    41881 aaatgagacc aaaattcaag tcatcaagat accaagccgt caagcaactc aaaggaagat
    41941 aggaaatcga acgacggagc gactgctcct agggagattt atctttttcc acagagttgt
    42001 aggcaagttc agttttcaag atacatcatt agaaaggttt aatactaaag tatctaaagc
    42061 tttcacgcta atcgctatcg ggcgattagc taaatgcttt actaactctc tcgtcaaata
    42121 acatcgattt gactcactcg tgtcgttaaa tcttacagtt taaatgcatt gtattattta
    42181 ataccttcat ctttgtatca agtacgtaca gaatctattt tatcatattt ttcttaaaaa
    42241 gtgaggtctt taccattaaa aaggaaccat tcccctcacc tgagaagaat ggtttgcttt
    42301 tattatccta gagactggtg attaaacaag gcatgggttg cttgatggat gtattttgct
    42361 gtatcagcat tattcatcgt atagagatgc acaccggcaa catcctgagt taccaagtcc
    42421 acgatttggt ccactgcata ggcaagtcct gctgctctga gcgactcagg gtcatgctca
    42481 tacttgtcta agatggcttt aaatttgcgt ggaagatgga tattctcaca agtcttcaag
    42541 agtcggagag cctgatttcg attcagaatt ggcataattc ctgcatgaat gggaacatca
    42601 atcccagcca agatgcactt gtcctgaaaa tcatagaagc gctcattgtc aaagaagagc
    42661 tgagttacga ggctcgaaca gcctgcatcc actttcttct taagattttg aatatctgaa
    42721 atctgatttg gcgaatctgg atgcccttct ggatagcaag ctccaataat atcaaagtga
    42781 ggggtttgtt ccttgataaa ctcaatcaag tcggttgcat agcggaaatc cttttgtggt
    42841 tccacgtctg gaataatatc cccacgaaga gccaagattt tctgcacccc aactttgtcc
    42901 aagtcagcaa tagtttcagc aaccttgtcc ttagttagat aaatagctgg caagtgggca
    42961 atggtcggaa tcgccaaatc attttggata aagtcagcca aacgaaccgt cgtttccttg
    43021 atattaaatt tattattgct ggcagttata ctgataaaat ggggagccaa ctcctgcata
    43081 tactgcaagg ctgaaataat gttaccatta cccacggctg ggtttggagg gaacacttca
    43141 aatgagagtg acggtgtttg gcgtgacata tgtaataacc ttttctagtt gatttctttt
    43201 tgaacaacca ctgtatggag agaaatccaa tcttacaatt tctcacgcgc agctttagct
    43261 gcttcaacaa ggcggatcaa gctttctttt gtttctggga taccacgtgt tttcaaacca
    43321 cagtcagggt tgatccaaac tttcttgctt ggcactttag caaggatggc ttcgattgtg
    43381 ttgtcgattt cgccttcatt tggtacacga ggtgagtgga tatcgtaaac cccaggtccc
    43441 acttctgttt ggaagttttt cgctttgagt tcgtccaaga tttcaaggtt tgaacggcta
    43501 gcttcaaagg aaataacgtc tgcatccatg ttatcgatag ctgggatgat atctgtaaat
    43561 tctgagtaac acatgtgagt gtggatttgt gtgtctggcg ctactgttga gtgtaccaag
    43621 cggaaggcag gaatagccca gtcaaggtag tcttcgtacc agtcgctacg gcggagtggc
    43681 aatttttcac gaagagcagc ctcgtcgatt tggatgattt tcacaccagc agcttcaagg
    43741 tcaagtactt catccttgat agcaagggcg atttggagag ttgaatcctt gatagagatg
    43801 tcttcacgtg ggaatgacca gttaaggatg gtaacaggtc cagtcaacat acctttaaca
    43861 ggtttgtttg tacgactttg tgcatagcta gaccatttaa cagtgatagg gttaagacga
    43921 gtgacatcac cccagatgat tggtggtttt accccacgca taccgtatga ttgtacccat
    43981 ccatttttag agaagaggta tcctgacaag ttttgaccga agtactcaac catgtcatta
    44041 cgctcaaatt caccgtgaac aaggacatca aagtcaatat cttcttgcca cttgatccat
    44101 tcgtcaatcg tttcagcaag gaaagcatcg tactcttttt gagacaattc acctttacgg
    44161 taagccaaac gtttggcacg aacttctttt gtttgaggga atgaaccaat cgttgttgtt
    44221 ggaagagctg gaagtttgaa agcttcttct tggatagctt cacgttctgc aaaggctggc
    44281 aaacgagtgt agtctgtgtc tgtcaagcca gcgatacgcg cacgaagttc agcattttca
    44341 ccaacacgct cagtcgcaaa gagttctttg ttggctgcaa gagcttctga accttgacca
    44401 ttgcggatag catccaagtc acggatttca tccaattttt caactgcaaa ggcaaagtgg
    44461 ttcaagagtg ctggttcaaa ttcttcatta gcagttgtaa atggcacatg aagaagtgag
    44521 caagagcttg tcaaaacgat gttttcagct gggatttgct caagaacagc caagctcttt
    44581 tcatagttgt tgcgccagat gtttttacca ttgacaatac ctgcatagag agtcttgtca
    44641 gctgggaagc cacctttaac gagttcaaga gttttcttac cttcaacaaa gtcaaaaccg
    44701 atagcatcta ctggcaagtt tacaaggtca gcgtatacgt cacgaacatc accgaaataa
    44761 gtttgaagca agacttcaag accttttttg tcagccaaga gtttgttgta aagattcaag
    44821 aagagagctt tttcttcagc tgtcaagtct tttacaagag ccgcttcatc caattggatg
    44881 caagtcgcac caagttcagc caatttagca aaaacttctt ggtaagcagc cactaagcta
    44941 tctacgaagt cgtctgcttt cacgccttct tcaaagtctg acaattgaag gaaagtgaag
    45001 ggacctacaa gaacaggacg agtgttcaat ccaagttctt tggcttcttg gaactcatca
    45061 aaaatcttgt gaccagccaa ttttacttga gtgtcttttt caaatttagg aacgatgtag
    45121 tggtagttag tgttaaacca tttcttcatt gggagggcgc gaacgtcccc tttttctccc
    45181 tggtaaccac gtcccaaagc gaagtagcgc tcaaggtcag acaagtccaa gttttgaacg
    45241 gatgcaggca ccacgttgaa aaggaaagcc gcatctagga agttatcata gtgagaaaag
    45301 tcatttgatg gaatttcagt gatgcctttt tctttgacaa tgttccagtg tttagcacgc
    45361 aagtcttttg ctgctgctaa aagttcttct tctgagattt cttttctaaa gtatttttca
    45421 gttgtaaatt ttaattcacg gaattcgccc aaacgaggga aaccgacgat tgtagttgac
    45481 atgatgtgtc ctccaaaatt tgttgttgaa actatcttaa cagaaaagaa agcgtctgta
    45541 taattgtaaa aaattagggt ttgatatagt ttgaaactat atatctgttt cggacaaaag
    45601 aaaaagactt gaagcaaacg tctcaaatcc tttgtatagc ttgttttata gctttatttc
    45661 agttagacgg ttcaaacgcg ttattagtaa ttcttataag tgactatggc ttgttattag
    45721 aaaagactat aactgattct agtcaacttt ttccctgttc aagtgggacg actgctagtg
    45781 tctttcctaa actggctagg acttttaaga ctgtatccaa ctgaggacta gtctttcctg
    45841 tctccatcct agctatgaca ggctggctta ttccactgac ttcttccagc tttttctgac
    45901 tgattccttg ttcataccta gcctcaatca actcgctcat gatagccact cgcatatcac
    45961 tttcaaggat ttcctccttg ctaaagagct cagatggaca tccttccaat tactcccaat
    46021 agcactattc ttcatcactt aaccctcttt tttttacgtc tatgtatttt taaaaaaatg
    46081 agcgaattat gattcgatag attgaccagt gggtttaaag ttggtgctag cctatttctt
    46141 aagcgatttt ccttttctag gataaagcat atcctgcttg cttaacccca attttccacg
    46201 atgaatccaa tagtaaatgg ttgaaattcc cacgttaacc cctttaacca taaccatcat
    46261 ttcaggcgaa aatttttggt tatgtatagt ggagaatctt ttcctttagt ttcttaagac
    46321 tgttgagcgt agtcggcaga ataaatctct ttgaagcgcc cttttccaag acattgtcgg
    46381 actgtcccac gctttatttc agtgtggata gtttgaggag cttttccaag tagagaggca
    46441 atttctctat ttgattttcc ttcttttttc catcgttcga ttaagcgacg gctatcgatt
    46501 gtcaaatgtt tgccttttgt agtataattg tcttgcattt ctgtgccttt taatcatttc
    46561 aatcttaaat tggacttttt ttacttgggt tgtacttaat ctataaggaa gacaataaaa
    46621 agaatatcaa tcaagtaaag tcacaaagtc acattagctc cgagcaacca ttgcaaatcg
    46681 aggtactcac acaatgatta aaacatttct ctctgccctt tcggtcattc tctttgttat
    46741 ccctatcata gcttattctt tttttccatc ttctaatctt aatatttggc tatctaccag
    46801 acctatcttg gcacagattt atgccttccc cttagctact gcaactatgg ctgctatttt
    46861 aagtttctta ttttttttcc tatcttttta caagaaaaat aaacaaatac ggttttactc
    46921 tggcattttg ctcttactat cgctcatatt actattattc ggaacagata aaaccctttc
    46981 ttctgcatca aataagacta aaaccttaaa attagtaact tggaacgtcg ctaatcaaat
    47041 agaagcacaa catattgagc gaatttttag ccattttgac gccgatatgg ctatattccc
    47101 tgaactagct accaatatca gaggtgagca agaaaaccag agaatcaaac tattgtttca
    47161 tcaagttgga ctttctatgg ccaactatga tattttcact tctccaccta ccaatagtgg
    47221 aatagctcct gtgactgtga ttgtcaagaa aagttatggt ttctatacag aagctaaaac
    47281 ttttcataca acacggttcg ggacaattgt attacattcg agaaaacaaa atataccaga
    47341 tatcattgcc ttgcatactg ctcctcctct gccaggttta atggaaatct ggaagcaaga
    47401 cttaaacatc attcataatc aattggcttc aaaatatcca aaggctatta ttgcaggtga
    47461 ttttaatgca actatgcgtc atggagcact tgcaaaaata agctctcata gggacgcatt
    47521 aaatgtactg ccaccttttg aaagaggaac ttggaatagc caaagtccaa aactttttaa
    47581 tgcaacaata gatcatattt tattgcctaa aaaccactac tatgttaaag atttagacat
    47641 tgtaagtttt caaaactctg atcatagatg tatttttaca gaaatcacat tttaattatt
    47701 ttatataaaa tcacccctct agtgttcata aactagaggg ggaatttgta tcatactatc
    47761 gtttaacgca cttctgcatt gactttttct tcgagcgatg cttggatttt ttccatatag
    47821 cgtgcgactt cttcgtccgt taagctatct tctggatttt ggaaggtcaa gctataagcc
    47881 attgacttca taccaagtcc caatttctca cctgagaaga cgtcaaagag tttgatatct
    47941 gtcaaacgtt tcacgccggc agcttggata gcatctacaa cttcttgatg agtcacttct
    48001 gccttgagga gaagggcaac gtcacggctg actgccggga atttggtgat ttctacaaat
    48061 ggagtcgctg gctgaagcgc agcttcgata gctgaaaggt taagctcagc cacatacgtt
    48121 tctggaatat cgtaagcctt ggcagtgact ggatgcactt ggccaaggaa accaagaact
    48181 tggtcaccga gtgaaatcac ggctgtacga cctggatgaa ggctagcgat ttcagatgtt
    48241 gctgtatagg ttacttggag tcccaaacga gtaaataggg cttcaaggat tcccttagca
    48301 tagaagaaat caactggaac tgctgctgtt tggaaatctt tttcagcaac caagcctgtc
    48361 aaggcaaagg caaaactgtt gatttcattt ggaagttctt cttttggatt acctgtttgt
    48421 tcaaagactt ttccaatctc gtaaagggcc aagtttttat tcttacgagc cacgttgtag
    48481 gcaacggtat caaggatacc tgaaatcata ttttgacgga ggactgaacg atccacagtc
    48541 attggccaca tgagttccgt aaggttactt ggttgagccg taaactcaac tgctttttca
    48601 ggagttgtta gagtataggt gatgatttct gtcaaacctg ctccttcagc aatagtacga
    48661 acttgacggc ggagtttttg tgtggctgtc aattcacctg ctgtaccatc gtcttttggt
    48721 agactagttg gcaagcggtc ataaccatag atacgagcaa tttcttcaaa gaggtcagct
    48781 tcgattgtga tatcccaacg acgacgtggg actctgactg taaagctgtc tgcatttcca
    48841 gaaagaccaa agccaagacg acggaagacg tcttctacat cagcataaga cagctcagtt
    48901 ccgaggacac ggttaacatc agcaagggtt gaagaaactt ctacatctga agtatcaagc
    48961 tcacccgctg aaacgatgcc cttacgcacc gtcgcacctg caagttccgc aatcaggcta
    49021 gctgccgcat caagggcttc attaactgtt gccacattaa ttcctttttc aaagcgagaa
    49081 gatgactcag aacgaaggtt caggcgacca cttgtcttac ggatagattt gccattgaaa
    49141 acagcagctt caaggacaac acgactagat ttttcagaga tttctgttgc ttgaccaccc
    49201 atgacacctg caagggctac tggcttgtct gcgacagtga tgactaggtc attcacgtcc
    49261 aagtcacgtt cttcaccgtc caaggtcacc aatttttcac cagcacgcgc ttcacgcaca
    49321 cggatgtcag tcccttcaaa gttatccaag tcaaaggcat gcattggttg accaaaatag
    49381 agcaggatgt agttggtcac gtccactacg ttattgatgg gacggattcc ttcgttcatg
    49441 agaaggtttt gcaaccattg tggacttggt gcgatggtca cattgtccaa gatacgagct
    49501 gcatagtaag gcgccttgtc tgtctcaatg ctgacagaaa gggcatctgc cgcagcttca
    49561 ttagtttctg ttagagtaaa ttctttaaag ttgactgcct tgtcatagat ggctgccact
    49621 tcgtgagcca ctccacacat agaaagggca tctgcacggt ttggtgtgat ggaaagttcg
    49681 atgatttcat catccaagtc taggtaagaa aagacttcct cacctggcac ggcatcttca
    49741 ggcaagattt ggatgccatc tgcgaattcc ttaggcacaa ctgagtcaga aattcccaat
    49801 tcaccaagtg aacagatcat tccaagtgac tccaaaccac ggatttttcc ttttttgatt
    49861 ttgtagttat cagcgatacg agctcctgga agagccacca tgaccttgat cccagcacgc
    49921 acatttgggg caccacaaac gatctgacgc tcttcttcgc caacgttaac ctgacaaaca
    49981 tggaggtgag tctctggcac atcttcgcaa gacaagacct caccgacgac aatttttgag
    50041 agaccagcag ctggtgattc gacaccctct acctcgattc ctgtagttga cattttttca
    50101 gccaactctt gtgatggcac atcaatgtcc accaattctt ttaaccattt ataagatata
    50161 agcataattt agttctccag aatgaccatt gccacactgg ttcttttcct ttcctatcat
    50221 ttcaatagaa gaatcctctt cttaccttaa tttctttctc agtaaccaat ccgtatctac
    50281 tttttgacca accataaaat gatgttggct aaatttttca aaaccatatc gattataaaa
    50341 ggcttgagct tttgtattat gctcccaaac acctagccaa gcccaagaaa aactattttt
    50401 tgtagcaagt tcaagtgcga attcaaacag ttgcttacct agtccaaatc cttggaattt
    50461 ttgtagcaca tagaggcgtt gaatttcaaa agcgtcctct aattctctct cagtttgagc
    50521 acttccccag ttgactttga gaaaaccagc tatctcctcc tcatgcataa tgaaataggt
    50581 ttcagagtca ggatttccca actcagttga caaagttttc agactataag cctcttcaaa
    50641 gtattcctgt aactgctctt ccgtattatc atacgcaaag gtttcacgaa aggtttgttt
    50701 ggcaatttta gccaacacct caacatctgc catttctact tttataatca ttatttaaac
    50761 tgttctgaga agcggacatc tccttggtag aatccacgga tatcgttgat tccataacgg
    50821 agcatagcta cacgctcttg tccaagacca aaggcaaagc cagagtatac agtcgcatcg
    50881 ataccactca tttcaaggac acgtgggtga accataccgg cccccataat ttcgatccaa
    50941 cctgttttct tacatatatt acagccttct ccaccacact tgaagcaaga aacatccacc
    51001 tcaacagatg gctctgtgaa tgggaagtaa gatggacgca aacgaatttg acgctcttca
    51061 ccaaacattt tttggacaat caactgaagc gttccttgaa gatcagccat agagatattt
    51121 ttcccaacta ccaagccttc gatttggtgg aattggtgac tgtgggtcgc atcgtccgta
    51181 tcgcgacgga agacacgccc tggcgagatc atcttcaaag gacctttaga aaaatcatgg
    51241 gcatccatag cacgcgcctg aactggagac gtgtgggtac ggagcaagat ttcttcagtg
    51301 atatagaaag tatcctgcat atcacgagct gggtggtctt ttggaaggtt catacgttca
    51361 aagttatagt agtcttgctc cacttcaaaa ccatccacga cttgataacc cataccgatg
    51421 aagatatctt cgatttcttc actggtttgt gtcaaaacgt gacggtgacc agtcgcaact
    51481 ggacgacctg gaagcgtcac atcgatactc tcgctagcca gttgagccgc gactttcttt
    51541 tcttccaaga gcttagctgt ttcttcaaaa gcagcagtca agacatcacg agcttcattg
    51601 acgtgtttcc cgatgattgg acgcatctca gcagaaacat ctttcatccc tttgaggatt
    51661 tcagtgagcg aacccttttt accaaggaca gagacacgca aatcttgcat ctctttttca
    51721 tttccagcag taatctgctt caagctagcc agcgtttctt cgcgaagcgc ttttaattgt
    51781 tcttcaatag ttgacatatt tcctccatca gtctctcgta gataaaaaga aaaccacatg
    51841 ccaaaaactc cactcggagc gttgacacgc ggtaccatcc gttttcatct gacaagtcag
    51901 accttcattt ctaaatccat gcgcaagtga attcacccag ctttcatata gagagcttgc
    51961 agtcacggct ctcctccctg atatacttcc cttgggctac tagtctttca gattcctatt
    52021 caattactac ttagtttatc agatttttac cattcttgca agacctatct tacttctgct
    52081 tgttagctta ttcttatcta actttatagc ggattgaaat aagatga
//
LOCUS       DAVMAF010000006        49116 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMAF010000006 DAVMAF010000000
VERSION     DAVMAF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.006"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            205..876
                     /locus_tag="VYO97_000322"
     CDS             205..876
                     /locus_tag="VYO97_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1753189.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(928..1125)
                     /locus_tag="VYO97_000323"
     CDS             complement(928..1125)
                     /locus_tag="VYO97_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753190.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1118..2026
                     /locus_tag="VYO97_000324"
     CDS             1118..2026
                     /locus_tag="VYO97_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1753191.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2023..2484
                     /locus_tag="VYO97_000325"
     CDS             2023..2484
                     /locus_tag="VYO97_000325"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1753192.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2474..3361
                     /locus_tag="VYO97_000326"
     CDS             2474..3361
                     /locus_tag="VYO97_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1753193.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEYVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3364..5247
                     /gene="cbpE"
                     /locus_tag="VYO97_000327"
                     /gene_synonym="pce"
     CDS             3364..5247
                     /gene="cbpE"
                     /locus_tag="VYO97_000327"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1753194.1"
                     /translation="MKKKLTSLALVGAFLDLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5327..6457
                     /locus_tag="VYO97_000328"
     CDS             5327..6457
                     /locus_tag="VYO97_000328"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1753195.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEVEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6467..7729
                     /locus_tag="VYO97_000329"
     CDS             6467..7729
                     /locus_tag="VYO97_000329"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1753196.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7733..8530
                     /gene="proC"
                     /locus_tag="VYO97_000330"
     CDS             7733..8530
                     /gene="proC"
                     /locus_tag="VYO97_000330"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1753197.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     SSLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8750..9388
                     /locus_tag="VYO97_000331"
     CDS             8750..9388
                     /locus_tag="VYO97_000331"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1753198.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9385..10275
                     /locus_tag="VYO97_000332"
     CDS             9385..10275
                     /locus_tag="VYO97_000332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1753199.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFESLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10315..10632
                     /gene="yabA"
                     /locus_tag="VYO97_000333"
     CDS             10315..10632
                     /gene="yabA"
                     /locus_tag="VYO97_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1753200.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10635..11504
                     /gene="rsmI"
                     /locus_tag="VYO97_000334"
     CDS             10635..11504
                     /gene="rsmI"
                     /locus_tag="VYO97_000334"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1753201.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11731..12249
                     /locus_tag="VYO97_000335"
     CDS             11731..12249
                     /locus_tag="VYO97_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1753202.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            12358..13002
                     /locus_tag="VYO97_000336"
     CDS             12358..13002
                     /locus_tag="VYO97_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1753203.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVRL
                     GLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYIE
                     NNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(12991..13795)
                     /locus_tag="VYO97_000337"
                     /pseudo
     CDS             complement(12991..13795)
                     /locus_tag="VYO97_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            13805..14045
                     /locus_tag="VYO97_000338"
                     /pseudo
     CDS             13805..14045
                     /locus_tag="VYO97_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
     gene            14290..15624
                     /gene="trmFO"
                     /locus_tag="VYO97_000339"
     CDS             14290..15624
                     /gene="trmFO"
                     /locus_tag="VYO97_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1753204.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            15708..16445
                     /locus_tag="VYO97_000340"
     CDS             15708..16445
                     /locus_tag="VYO97_000340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1753205.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            16454..17011
                     /gene="frr"
                     /locus_tag="VYO97_000341"
     CDS             16454..17011
                     /gene="frr"
                     /locus_tag="VYO97_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1753206.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            17071..17925
                     /gene="cvfB"
                     /locus_tag="VYO97_000342"
     CDS             17071..17925
                     /gene="cvfB"
                     /locus_tag="VYO97_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1753207.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            17934..18149
                     /locus_tag="VYO97_000343"
     CDS             17934..18149
                     /locus_tag="VYO97_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1753208.1"
                     /translation="MRKSFYIWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            18235..19203
                     /locus_tag="VYO97_000344"
     CDS             18235..19203
                     /locus_tag="VYO97_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1753209.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(19181..19360)
                     /locus_tag="VYO97_000345"
     CDS             complement(19181..19360)
                     /locus_tag="VYO97_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753210.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            19396..19896
                     /locus_tag="VYO97_000346"
     CDS             19396..19896
                     /locus_tag="VYO97_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753211.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            19899..20158
                     /locus_tag="VYO97_000347"
                     /pseudo
     CDS             19899..20158
                     /locus_tag="VYO97_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(20525..20851)
                     /locus_tag="VYO97_000348"
     CDS             complement(20525..20851)
                     /locus_tag="VYO97_000348"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753212.1"
                     /translation="MVDLAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(20797..21210)
                     /locus_tag="VYO97_000349"
                     /pseudo
     CDS             complement(20797..21210)
                     /locus_tag="VYO97_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21211..21636)
                     /locus_tag="VYO97_000350"
     CDS             complement(21211..21636)
                     /locus_tag="VYO97_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1753213.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYFRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVADRMAV"
     gene            21813..22382
                     /locus_tag="VYO97_000351"
     CDS             21813..22382
                     /locus_tag="VYO97_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753214.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            22450..23100
                     /locus_tag="VYO97_000352"
     CDS             22450..23100
                     /locus_tag="VYO97_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1753215.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            23084..25324
                     /locus_tag="VYO97_000353"
     CDS             23084..25324
                     /locus_tag="VYO97_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1753216.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGEKLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTIKEGKGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKGSLKQKQFVVELQATKTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            25723..26310
                     /locus_tag="VYO97_000354"
     CDS             25723..26310
                     /locus_tag="VYO97_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753217.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            26314..27489
                     /locus_tag="VYO97_000355"
     CDS             26314..27489
                     /locus_tag="VYO97_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753218.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEAKLSQMSDTSSEEYQFAKSNLELQKNLLKRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIIWILEAIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVKQEVTIGKMQDVLFPS
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    27663..27794
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            27810..28340
                     /gene="infC"
                     /locus_tag="VYO97_000356"
     CDS             27810..28340
                     /gene="infC"
                     /locus_tag="VYO97_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1753219.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            28373..28573
                     /gene="rpmI"
                     /locus_tag="VYO97_000357"
     CDS             28373..28573
                     /gene="rpmI"
                     /locus_tag="VYO97_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1753220.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            28625..28984
                     /gene="rplT"
                     /locus_tag="VYO97_000358"
     CDS             28625..28984
                     /gene="rplT"
                     /locus_tag="VYO97_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1753221.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            29042..29422
                     /locus_tag="VYO97_000359"
     CDS             29042..29422
                     /locus_tag="VYO97_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1753222.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            29672..30472
                     /locus_tag="VYO97_000360"
     CDS             29672..30472
                     /locus_tag="VYO97_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1753223.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGHVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            30483..31421
                     /locus_tag="VYO97_000361"
     CDS             30483..31421
                     /locus_tag="VYO97_000361"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1753224.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSTEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            31463..33628
                     /gene="lytB"
                     /locus_tag="VYO97_000362"
     CDS             31463..33628
                     /gene="lytB"
                     /locus_tag="VYO97_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1753225.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFY
                     LKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAA
                     YYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQ
                     ALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLE
                     NPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHS
                     ALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGR
                     TFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(33689..35344)
                     /gene="pavA"
                     /locus_tag="VYO97_000363"
     CDS             complement(33689..35344)
                     /gene="pavA"
                     /locus_tag="VYO97_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1753226.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLETDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            35449..35946
                     /gene="ybeY"
                     /locus_tag="VYO97_000364"
     CDS             35449..35946
                     /gene="ybeY"
                     /locus_tag="VYO97_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1753227.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            35927..36322
                     /locus_tag="VYO97_000365"
     CDS             35927..36322
                     /locus_tag="VYO97_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1753228.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            36339..37238
                     /gene="era"
                     /locus_tag="VYO97_000366"
     CDS             36339..37238
                     /gene="era"
                     /locus_tag="VYO97_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1753229.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            37287..38111
                     /gene="mutM"
                     /locus_tag="VYO97_000367"
     CDS             37287..38111
                     /gene="mutM"
                     /locus_tag="VYO97_000367"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1753230.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            38111..38716
                     /locus_tag="VYO97_000368"
     CDS             38111..38716
                     /locus_tag="VYO97_000368"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1753231.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            38703..39902
                     /locus_tag="VYO97_000369"
     CDS             38703..39902
                     /locus_tag="VYO97_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1753232.1"
                     /translation="MTEINWKDNLRIAWFDNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            39902..40051
                     /gene="rpmG"
                     /locus_tag="VYO97_000370"
     CDS             39902..40051
                     /gene="rpmG"
                     /locus_tag="VYO97_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1753233.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            40091..40324
                     /gene="secG"
                     /locus_tag="VYO97_000371"
     CDS             40091..40324
                     /gene="secG"
                     /locus_tag="VYO97_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1753234.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            40426..42780
                     /gene="rnr"
                     /locus_tag="VYO97_000372"
     CDS             40426..42780
                     /gene="rnr"
                     /locus_tag="VYO97_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1753235.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKYSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTQ"
     gene            42743..43210
                     /gene="smpB"
                     /locus_tag="VYO97_000373"
     CDS             42743..43210
                     /gene="smpB"
                     /locus_tag="VYO97_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1753236.1"
                     /translation="MAKGEGKVVAHNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            43226..44086
                     /gene="tehB"
                     /locus_tag="VYO97_000374"
     CDS             43226..44086
                     /gene="tehB"
                     /locus_tag="VYO97_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1753237.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            44159..45112
                     /gene="coiA"
                     /locus_tag="VYO97_000375"
     CDS             44159..45112
                     /gene="coiA"
                     /locus_tag="VYO97_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1753238.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            45131..46933
                     /gene="pepF"
                     /locus_tag="VYO97_000376"
     CDS             45131..46933
                     /gene="pepF"
                     /locus_tag="VYO97_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1753239.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            46935..47648
                     /locus_tag="VYO97_000377"
     CDS             46935..47648
                     /locus_tag="VYO97_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1753240.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            47715..48656
                     /gene="prsA"
                     /locus_tag="VYO97_000378"
     CDS             47715..48656
                     /gene="prsA"
                     /locus_tag="VYO97_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1753241.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(48773..>49116)
                     /locus_tag="VYO97_000379"
     CDS             complement(48773..>49116)
                     /locus_tag="VYO97_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1753242.1"
                     /translation="MLEQAFKEKHYENTILHSDQDWQYQHDSYHRFLESKGIQASMSR
                     KGNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGL
                     SPVQYRTKSFG"
BASE COUNT        14807 a         8906 c        11154 g        14249 t
ORIGIN      
        1 atatggtaaa aatcctaatg gtaggtacga ttgggaaata gaaccagtat taactctgcc
       61 ggttcatgga ttttgtccca gaggcaccta tgatttagga tatattggag gaggcaatca
      121 tctttgcaaa ggaagtgctg cgagatttta agtaaaattt attagtaata tgaagaaaca
      181 aggggagaaa gcagagaatt taatatgaaa aaacgggcta ttcaaatttt actagcattg
      241 tccttaattt tttacaaatc aacttggttt tggagagttt tcaatcacct cgcaaagccc
      301 tatctaccag caagtcgtga attttttcag attctgcttt tgatggagag cggagttctt
      361 ttcttagcgg tcatctatct actggttttt gcaggaaaga aaatttttca tttcaagtgg
      421 cagctgaggt acttcatcta ccttttactg ggctacatca tttcatatat gtctgacttc
      481 ctcttttcgt atttcatatc cctgtcttca aatcagattt ctttgaatga aacgatagaa
      541 atgatgggga gacaggagtt cccttatttc ttgctcatcg tttgcttcat cgcccctatt
      601 gctgaggaat tgatttatcg aggtgtgctt atgacaacct ttttcaaaaa ctcaccttgg
      661 tacggagatg ttttgctttc tgctattatt ttcggttata ttcatatcaa ttttgcttta
      721 actcctcttg cttttttcat ttatgctagt ggaggtctga ttttagctct attgtatcgc
      781 atgactaaaa atctctacta tccaatacta gttcatattc tcatcaatat cactgccttc
      841 tgggatgttt ggttactcct attttcagga agttagctta ctaaaaaaat gtcggaattt
      901 tccggcattt tcttttttca caaatagtca acgtttttct ttccgatact gaagtggtgt
      961 gtagccactt atttttttga attgattttg aaaataagat tggcgtgaga aaggcagata
     1021 gtgaagatag ttaagaagaa taggatgttc ttttttcctt tttggaaaac ttctaaaata
     1081 tggtataatg aaaagataaa gaagttgggg gtagaagatg aacattcaac aattacgcta
     1141 tgttgtggct attgccaata gtggtacttt tcgtgaagct gctgaaaaga tgtatgttag
     1201 tcagccgagt ctgtctattt ctgttcgtga tttggaaaaa gagttgggct ttaagatttt
     1261 ccgtcggacc agctcaggga ctttcttgac ccgtcgtggg atggaatttt atgaaaaagc
     1321 gcaagaattg gttaaaggat ttgatatttt tcaaaatcag tatgccaatc ctgaagaaga
     1381 aaaagatgaa ttttccgttg ctagccagca ctatgacttc ttaccaccaa ctattacggc
     1441 cttttcagag cgctatcctg actataagaa cttccgtatt tttgaatcaa ctactgttca
     1501 aatattagat gaagtggcgc aagggcatag tgagattggg attatctacc tcaacaatca
     1561 aaataaaaag gggattatgc aacgggttga aaagttaggt ctggaggtca tcgaattgat
     1621 tcctttccat acccatattt atctctgtga gggtcatcct ttagcccaga aagaggaatt
     1681 agtcatggag gatttagcgg atttaccaac ggttcgtttc actcaagaga aagacgagta
     1741 cctttattat tcagagaact ttgtcgatac cagcgctagc tcacagatgt ttaatgtgac
     1801 agaccgtgcc accttgaatg gtattttgga gcggacggac gcctatgcga caggttctgg
     1861 atttttagat agtgacagtg ttaatggcat tacagttatt cgtctcaagg ataacctaga
     1921 taaccgcatg gtctatgtta aacgtgaaga agtggagctt agtcaagctg ggactctctt
     1981 cgtagaagtc atgcaagaat attttgatca aaagaggaaa tcatgaaaaa aagagcaata
     2041 gtggcagtca ttgtactgct tttaattggg ctggatcagt tggtcaaatc ctatatcgtc
     2101 cagcagattc cactgggtga agtgcgctcc tggattccca atttcgttag cttgacctac
     2161 ctgcaaaatc gaggtgcagc cttttctatc ttacaagatc agcagctgtt attcgctgtc
     2221 attactctgg ttgtcgtgat aggtgccatt tggtatttac ataaacacat ggaggactca
     2281 ttctggatgg tcttgggttt gactctaata atcgcgggtg gtcttggaaa ctttattgac
     2341 agggtcagtc agggctttgt tgtggatatg ttccaccttg actttatcaa ctttgcaatt
     2401 ttcaatgtgg cagatagcta tctgacggtt ggagtgatta ttttattgat tgcaatgcta
     2461 aaagaggaaa taaatggaaa ttaaaattga aactggtggt ctgcgtttgg ataaggcttt
     2521 gtcagatttg tcagaattat cacgtagtct cgcgaatgaa caaattaaat caggccaggt
     2581 cttggtcaat ggtcaagtca agaaagctaa atacacagtc caagagggtg atgtcgtcac
     2641 ttaccatgtg ccagaaccag aggtattaga gtatgtggct gaggatcttc cgctagaaat
     2701 agtctaccaa gatgagtatg tggctgtcgt taacaaacct cagggaatgg ttgtgcaccc
     2761 gagtgctggt cataccagtg gaaccctagt aaatgccctc atgtatcata ttaaggactt
     2821 gtcgggtatc aatggggttc tgcgtccagg gattgttcac cgtattgata aggatacgtc
     2881 aggtcttctc atgattgcta aaaacgatga tgcgcatcta gtacttgccc aagaactcaa
     2941 agataaaaag tctctccgca aatattgggc gattgttcat ggaaatctgc ctaatgatcg
     3001 tggtgtaatt gaagcgccga ttggccggag tgaaaaagac cgtaagaaac aggctgtaac
     3061 tgctaaaggg aagcctgcag tgacgcgttt tcacgtcttg gaacgctttg gcgattatag
     3121 cttagtagag ttgcaactgg agacagggcg cactcatcaa atccgtgtcc acatggctta
     3181 tatcggccat ccagtcgctg gtgatgaggt ctatggtcct cgcaagactt tgaaaggaca
     3241 tggacaattt cttcatgcca agactttagg ttttactcat ccgagaacag gtaagacctt
     3301 ggaatttaaa gcagatatcc cagagatttt taaggaaacc ttggagagat tgagaaagta
     3361 agaatgaaaa agaaattaac tagtttagca cttgtaggcg cttttttaga tttgtcatgg
     3421 tatgggaatg ttcaggctca agaaagttca ggaaataaaa tccactttat caatgttcaa
     3481 gaaggtggca gtgatgcgat tattcttgaa agcaatggac attttgccat ggtggataca
     3541 ggagaagatt atgatttccc agatggaagt gattctcgtt atccatggag agaaggaatt
     3601 gaaacgtctt ataagcatgt tctaacagac cgtgtctttc gtcgtttgaa ggaattgggt
     3661 gtccaaaaac ttgattttat tttggtgacc catacccaca gtgatcatat tggaaatgtt
     3721 gatgaattac tgtctaccta tccagttgac cgagtctatc ttaagaaata tagtgatagt
     3781 cgtattacta attctgaacg tctatgggat aatctgtatg gctatgataa ggttttacag
     3841 actgctgcag aaaaaggtgt ttcagttatt caaaatatca cacaagggga tgctcatttt
     3901 cagtttgggg acatggatat tcagctctat aattatgaaa atgaaactga ttcatcgggt
     3961 gaattaaaga aaatttggga tgacaattcc aattccttga ttagcgtggt gaaagtcaat
     4021 ggcaagaaaa tttaccttgg gggcgattta gataatgttc atggagcaga agacaagtat
     4081 ggtcctctca ttggaaaagt tgatttgatg aagtttaatc atcaccatga taccaacaaa
     4141 tcaaatacca aggatttcat taaaaatttg agtccgagtt tgattgttca aacttcggat
     4201 agtctacctt ggaaaaatgg tgttgatagt gagtatgtta attggctcaa agaacgagga
     4261 attgagagaa tcaacgcagc cagcaaagac tatgatgcaa cagtttttga tattcgaaaa
     4321 gacggttttg tcaatatttc aacatcctac aagccgattc caagttttca agctggttgg
     4381 cataagagtg catatgggaa ctggtggtat caagcgcctg attctacagg agagtatgct
     4441 gtcggttgga atgaaatcga aggtgaatgg tattacttta accaaacggg tatcttgtta
     4501 cagaatcaat ggaaaaaatg gaacaatcat tggttctatt tgacagactc tggtgcttct
     4561 gctaaaaatt ggaagaaaat tgatggaatc tggtattatt ttaacaaaga aaatcagatg
     4621 gaaattggtt gggttcaaga taaagagcag tggtattatt tggatgttga tggctctatg
     4681 aagacaggtt ggcttcaata taaggggcaa tggtattact ttgctccatc aggggaaatg
     4741 aaaacgggct gggtaaaaga taaagaagcc tggtactata tggattctac tggtatcatg
     4801 aagacaggtg agatagaagt tgctggacat cattactatc tagaagaatc aggagctatg
     4861 aagcaaggct ggcttaaaaa ggcaaatgat tggtatttct acaagacaga tggttcacga
     4921 gctataggtt ggattaaaga taaggataaa tggtacttct tgaaagaaaa tggtcaatta
     4981 cttgtgaatg gtaagacacc agaaggctat actgtggatt caagtggtgc ctggttagtg
     5041 gatgttccga tcgagaaatc tgctacaatt aaaactacaa gtcattcaga aataaaagaa
     5101 tccaaagaag tagtgaaaaa ggatcttgaa aataaagaaa cgagtcaaca tgaaagtgtt
     5161 acaaattctt caactagtca agatttgaca tcctcaactt cacaaagctc tgaaacgagt
     5221 gtaaacaaat cggaatcaga acagtagtag aaaagaaggt tttagggcct tctttttcct
     5281 atcaactctt ttctatttcc tgttattcat gttataatgg ataaatatga ataatcggag
     5341 tgagactatg aaatacaaac ggattgtctt taaggtgggt acttcttctc tgacaaatga
     5401 ggatggaagt ttatcacgta gtaaggtaaa ggatattacc cagcagttgg ctatgctgca
     5461 cgaggctggt catgagttga ttttggtgtc ttcaggtgcc attgcggctg gttttggagc
     5521 cttaggattt aaaaagcgtc cgactaaaat tgctgataaa caggcttcag cagcggtagg
     5581 gcagggactt ttgttggaag aatatacgac caatcttctc ttgcgtcaaa tcgtttctgc
     5641 acaaatcttg ctgacccaag atgactttgt ggataagcgt cgttataaaa atgcccatca
     5701 ggctttgtcg gttttgctca accgtggggc aattcctatc ataaatgaga atgatagtgt
     5761 cgttattgat gagctcaagg ttggggacaa tgacactcta agtgctcaag tagcggcgat
     5821 ggtccaagca gaccttttag ttttcttgac agatgtggac ggtctctata ctggaaatcc
     5881 taattcagat ccaagagcca aacgcttgga gagaatcgag accatcaatc gtgagattat
     5941 tgatatggct ggtggagctg gttcgtcaaa cggaactggg ggtatgttaa ccaaaatcaa
     6001 ggctgcaact atcgcgacgg aatcaggagt tcctgtttat atctgctcat ccttgaaatc
     6061 agattccatg attgaggcgg cagaggagac cgaggatggt tcttactttg ttgctcaaga
     6121 gaaggggctt cgtacccaga aacaatggct tgccttctat gctcagagtc aaggttctat
     6181 ttgggttgat aaaggggctg cggaagctct ctctcaacat ggaaagagtc ttctcttatc
     6241 tggtatcgtt gaagtagaag gagccttttc ttacggtgat atcgtgacag tatttgacaa
     6301 ggaaagtgga aaatcacttg gaaaaggacg cgtgcaattt ggagcatctg ctttggagga
     6361 tatgttgcgt tctcaaaaag ccaagggtgt cttgatttac cgtgacgact ggatttccat
     6421 tactcctgaa atccaactac tttttacaga attttagagg taaactatgg tgagtagaca
     6481 agaacaattt gaacaggtac aggctgttaa aaaatcgatt aacacagcta gtgaagaagt
     6541 gaaaaaccaa gccttgctag ccatggctga tcacttagtg gctgctactg aggaaatttt
     6601 agcggctaat gccctcgata tggcagcggc taaggggaaa atctcagatg tgatgttgga
     6661 tcgtctttat ttggatgcag atcgtataga agcgatggca agaggaattc gtgaagtggt
     6721 tgccttacca gatccaatcg gtgaagtttt agaaacaagt cagcttgaaa atggtttggt
     6781 tatcacaaaa aaacgtgtag ctatgggtgt catcggtatt atctatgaaa gccgtccaaa
     6841 tgtgacgtct gatgcggctg ctttgactct taagagtgga aatgcggttg ttcttcgtag
     6901 tggtaaggat gcctatcaaa caacccatgc cattgtcaca gccttgaaga agggcttgga
     6961 gacgactact attcatccaa atgtgattca actggtggag gatactagcc gtgaaagtag
     7021 ttatgctatg atgaaggcca agggctatct agacctcctc attcctcgtg gaggagctgg
     7081 cttgatcaat gcagtggttg agaatgcgat tgtacctgtt atcgagacag ggactgggat
     7141 tgtccatgtc tatgtggata aggatgcaga cgaagacaag gcgctgtcta tcatcaacaa
     7201 tgctaaaacc agtcgtcctt ctgtttgtaa tgccatggag gttctgctgg ttcatgaaaa
     7261 caaggcagca agcatccttc ctcgcttgga tcaaatgctg gttgcagagc gtaaggaagc
     7321 tggactggaa ccaattcaat tccgcttgga tagcaaagca agccagtttg tttcaggtca
     7381 agcagctgag acccaagact ttgacaccga gtttttagac tatgtccttg ctgttaaggt
     7441 tgtgagcagt ttagaagaag cggttgcgca cattgaatcc cacagcaccc atcattcgga
     7501 tgctattgtg acggaaaatg ctgaagctgc agcatacttt acagatcaag tggactctgc
     7561 agcggtgtat gttaatgcct caactcgttt cacagatgga ggacaatttg gtcttggttg
     7621 tgaaatgggg atttctactc agaaattgca cgcgcgtggt ccaatgggct tgaaagagtt
     7681 gaccagctac aagtatgtgg ttgctggtga tgggcagata agggagtaag agatgaagat
     7741 tggatttatc ggtttgggga atatgggtgc tagcttggca aaatctgtct tgcagactag
     7801 gacgtcagat gagattctcc ttgccaatcg tagtcaagct aaggtagatg ctttcattgc
     7861 agactttggt ggtcaggctt ccagcaatga agaaatgttt gcagaagcag atgtgatttt
     7921 tctaggagtt aagcctgctc agttttctga actgctttct caataccaga ccatccttga
     7981 aaaaagagaa agtcttcttt tgatttcgat ggcagctgga ttgaccttag aaaaactatc
     8041 aagtcttatc ccaagccaac accgaattat tcgtatgatg cctaataccc ctgcttctat
     8101 cgggcaagga gtgattagtt atgccttgtc tcctaattgc agggctgagg acagtgagat
     8161 cttttgtcag cttttagcca aggctggtct cttggttgaa ttaggagaaa gcttaatcaa
     8221 tgcagcgaca ggtcttgcag gttgtggacc agcctttgtc tatctcttta ttgaggcctt
     8281 ggcagatgca ggtgttcaga cgggattacc acgagaaaca gccttgaaaa tggcagccca
     8341 aactgtggta ggagctgggc aattggtcct agaaagccag caacatcctg gagttttgaa
     8401 agatcaagtt tgtagtccag gcggttcgac tattgctggc gtagcaagcc tagaagcgca
     8461 tgcttttcga ggcacagtca tggaggcagt tcatcaagcc tataaacgaa cacaagaact
     8521 aggtaaataa gaggtagctt tgactgcctc ttttatggtg gttgatactc ttcgaaaatc
     8581 aaattcaaac tgcgtcagct tcaccttgcc gtactcaagt acagcctgcg gctagcttcc
     8641 tagtttgctc tttgattttc attgagtatg aaatgagaag acaaaaagat tgtcacaaac
     8701 ccctattttt ttgatagaat agaagtagta aaaaagaaat gagttagaca tgtcaaaagg
     8761 atttttagtc tctcttgagg gaccagaggg agcaggcaag accagtgttt tagaggctct
     8821 gctaccaatt ttagaggaaa aaggagtaga ggtgttgacg acccgtgaac ctggcggagt
     8881 cttgattggg gagaagattc gggaagtgat tttggatcca agtcatactc agatggatgc
     8941 taaaacagag ctacttctct atattgccag tcgcagacag catttggtgg aaaaagttct
     9001 tccagccctt gaagctggca agttggtcat catggatcgt tttatcgata gttctgttgc
     9061 ctatcaggga tttggtcgtg gcttagatat tgaagccatt gactggctca atcagtttgc
     9121 gacagatggc ctcaaacccg atttgacact ctattttgac atcgaggtgg aagaagggct
     9181 ggctcgtatt gctgctaata gtgaccgcga ggttaatcgt ttggatttgg aagggttgga
     9241 cttgcataaa aaagttcgtc aaggctacct ttctcttctg gataaagagg gaaatcgcat
     9301 tgtcaagatt gatgctagtc tccctttgga gcaagttgtg gaaactacca aggctgtctt
     9361 atttgacgga atgggcttgg ccaaatgaaa caagatcaac taaaggcttg gcaaccagct
     9421 cagtttgacc gttttgtccg tatcttagaa caagaccagc tcaatcacgc ctatctcttt
     9481 tcaggtttct ttgaaagctt ggaaatggcg caatttttag ctaagagcct cttttgtacg
     9541 gataaagttg gcgtcttacc atgtgagaaa tgccgaagtt gcaagctgat tgaacaggga
     9601 gagtttccag atgtcacctt gattaagcca gtcaatcagg tcatcaagac agaacgcatt
     9661 cgggaattgg tgggacagtt ttctcaagca gggattgaaa gccagcaaca ggtctttatc
     9721 atcgagcaag cggataaaat gcatcccaac gcagccaatt ctctgctcaa ggtcatcgaa
     9781 gaaccccaga gtgaagttta tattttcttc ttgactagcg atgaggaaaa gatgttaccg
     9841 acaatccgaa gtcggactca gatcttccac tttaaaaagc aagaagaaaa acttatctta
     9901 ctcttagaac aaatgggact tgttaagaaa aaagcgactc ttttagctaa gtttagtcaa
     9961 tcgcgagctg aagcagaaaa gttggctaat caggcaagtt tttggacctt ggtcgatgaa
    10021 agtgaacgcc tgctgacttg gttagtagct aagaaaaaag aaagttatct acaggttgcc
    10081 aaattagcca acttggcaga tgataaggaa aaacaggatc aggttttacg gattcttgaa
    10141 gttctctgtg ggcaggacct cttgcaggta agagtaagag tgattctaca agatttacta
    10201 gaagctagaa aaatgtggca agctaatgtc agctttcaaa atgccatgga atatctggtc
    10261 ttgaaagaaa tataaactca aaaatgaatg ataaagaaag gaaagggctg ttttatggac
    10321 aaaaaagaat tatttgacgc gctggatgat ttttcccaac agttattggt aaccttggcc
    10381 gatgtggaag ccatcaagaa aaatctcaag agcctggtag aggaaaatac agctcttcgt
    10441 ttggaaaatt ctaagttgcg agaacgcttg ggtgaggtgg aagcagatgc tcctgtcaag
    10501 gccaagcatg ttcgtgaaag tgtccgtcgc atttaccgtg atggatttca cgtatgtaat
    10561 gatttttatg gacaacgtcg agagcaggac gaagaatgta tgttctgtga tgagttgtta
    10621 tacagggagt aggcatgcaa attcaaaaaa gttttaaggg gcagtctccc tatggcaagc
    10681 tgtatctagt ggcaacgccg attggcaatc tagatgatat gacttttcgt gctatccaga
    10741 ccttgaaaga agtggactgg attgctgctg aggatacgcg caatacaggt cttttgctca
    10801 agcattttga catttccacc aagcagatca gttttcatga gcacaatgcc aaggaaaaaa
    10861 ttcctgattt gattggtttc ttgaaagcag ggcaaagtat tgctcaggtc tctgatgccg
    10921 gtttgcctag catttcagac cctggtcatg atttagttaa ggcagctatt gaggaagaaa
    10981 ttgcagttgt gacagttcca ggtgcctctg caggaatttc tgccttgatt gccagtggtt
    11041 tagcgccaca gccacatatc ttttacggtt ttttaccgag aaaatcaggt cagcagaagc
    11101 aattttttgg cttgaaaaaa gattatcctg aaacacagat tttttatgaa tcacctcatc
    11161 gtgtagcaga cacgttggaa aatatgttag aagtctacgg tgaccgctcc gttgtcttgg
    11221 tcagggaatt gaccaaaatc tatgaagaat accaacgagg tactatctct gagttattag
    11281 aaagcattgc tgaaacgcca ctcaagggcg aatgtcttct cattgttgag ggtgccagtc
    11341 agggtgtgga ggaaaaggac gaggaagact tgttcgtaga aattcaaacc cgcatccagc
    11401 aaggtgtgaa gaaaaaccaa gctatcaagg aagtcgctaa gatttaccag tggaataaaa
    11461 gtcagctcta cgctgcctac cacgactggg aagaaaaaca ataaagggag acaggatgta
    11521 ataattctgt ctgtttctgt ttaacttaat tagtgatggt aatataaaga tgtatcactt
    11581 ggtatagaag ctttggtatt aagtttttta ttaagcccat acggaatacc gatggttgga
    11641 gcagcagtta tagcgttctt agaaggtata aatagaaaaa taaggtcatt ttaaatcaaa
    11701 ggattgataa atcagaaaga aggtgatttt ttgcgaacat acgaaaataa agaagaacta
    11761 aaagctgaga tagagaaaac atttgagaaa tatattttag aatttgataa tattccagaa
    11821 aatttaaaag ataagagagc tgatgaagtt gacagaactc cagcagaaaa ccttgcttat
    11881 caggttggtt ggaccaactt ggttcttaaa tgggaagaag atgaaagaaa ggggcttcaa
    11941 gtaaaaacac catcggataa atttaaatgg aatcaacttg gtgaattata tcagtggttc
    12001 acagatacct acgctcattt atctctgcaa gagttgaaag caaaattaaa tgaaaatatt
    12061 aattctatct ctgcaatgat tgattcgttg agtgaggaag aattatttga accgcatatg
    12121 agaaagtggg ctgatgaagc gactaaaaca gcgacttggg aagtgtataa gtttattcat
    12181 gtaaatacgg ttgcaccttt tggaactttc agaactaaaa tcagaaaatg gaagaagata
    12241 gtattataaa ttatattttt aactttaaaa aatttcataa aaatggttac caaaggcgat
    12301 agaagaaaaa ctatcgtctt tttctttgca aatttttaag aagggaggtg atcttgcatg
    12361 gactttgaat atttttataa cagagaagcg gaaagattta acttcttaaa agtaccggag
    12421 atattagttg atagagaaga atttcggggc ttatcagcag aagcaattat cctttattcc
    12481 atacttctta aacagacagg aatgtcattt aagaataact ggatagacaa ggaaggcaga
    12541 gtatttatct ttactgtcga agaaattatg aaaagaagaa atatctcaaa gccaactgcc
    12601 ataaaaacat tagatgagct tgatgtaaaa aaaggaatag gactgatcga aagagtaagg
    12661 cttggacttg gtaagccgaa catcatttat gttaaagact ttatgagtat atttcaggta
    12721 aaagaaaatg acttacagaa gtcaaaaaac ttaacttcag aagtaaaaga ttttaacctc
    12781 agaagtaaag aaaatgaact tcaagaggtt aagaaccttg actctaacta tatagagaat
    12841 aataagagta agtatagtaa gagagaatat agttttggtg aaaacggact tggaacattt
    12901 caaaatgtgt ttttagctgc tgaagatata tcggatttac aaatcataat gaactcacag
    12961 cttgagaatt acattagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc
    13021 agcaatcaaa ttcattcgta atccgaagcg tttacgatga tttcgatagg ttgttgaaat
    13081 cattttaaac gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttggatag
    13141 cgcatggtta caggctttat cttcaactgt tagtggcttg agtttgctgg atttacgtgg
    13201 agtttgtgct tgaggatata tcttcatgag cccttgataa ttactgtcag ccaagatttt
    13261 accagcttgt ccgatatttc cgcgactcat tttgaacaac ttcatatcat gacaatagtt
    13321 cacagtgata tccaaagaaa taattcttcc ttgacttgtg acaatcgctt gagccttcat
    13381 agcgtggcat ttctttttac cagaatcatt cgctaattgt ttttttaggt cgattgattt
    13441 ttacttccgt cgcatcaatc attaccgtgt cctcaaaact aagaggagtt cttgaaatcg
    13501 taacaccact ttgaacaaga gttacttcaa cccattggct ccgacggatt aagttgcttt
    13561 cgtggatacc aaaatcagcc gcaatttctt cataagttca atattctcgc acatattgaa
    13621 gagtggccat aagaaggtct tctaggctta atttaggttt tcgtccacct tttgcgtgtt
    13681 taagttgata agctgttttt aatacagcta acatctcttc aaaagtggta cgctgaacac
    13741 caacaagacg cttaaatcgt gtatcagtta attgtttact tgcttcataa ttcatagaac
    13801 tactatacca tattttgttt cgcaggaagt ctattggaaa gtaagaaata ttgaagctga
    13861 ggctattaga agaaattgtg agcgtggtgc tattttttca ggtaaaaata aaatatcacg
    13921 aagattcaca gtttaaagga gatcactatg ttgaatgtta tgctgtttta gataatacgg
    13981 ttatagcaag agatagaata acagtcccta tcgatccgtt atgtggaaaa gattttatag
    14041 agtagcatat aattgattct taactggaat actcactatc tctttacatc aagaaaatga
    14101 ctaaacaggg aagtttgcct tcttcccttt ttttgttata ctagtagaag aaaaaataga
    14161 aagatttgtg ggagtgaaaa agtcctgtgg actttttcag cctgagccaa gaaactcgaa
    14221 agctcgtaag tctgattggc tttttcaatg tgaatcttaa cttcacactc ccaaagaggt
    14281 attagtgtcg tgtctcaatc ttatatcaat gttatcggtg ctggtttggc aggttctgaa
    14341 gcagcttacc aaatcgcaga gcgtggtatt ccagttaaac tttatgaaat gcgtggtgtc
    14401 aagtcgactc ctcagcataa aacagacaat tttgctgagt tggtttgttc caattctttg
    14461 cgtggggatg ctttgacaaa tgcagttggt cttctcaagg aagaaatgcg tcgcttgggt
    14521 tctgttatct tggaatctgc tgaggctaca cgtgttcctg ccggtggagc ccttgcggtg
    14581 gatcgtgatg gtttctccca aatggtaacc gaaaaagtgg tcaaccaccc cttgattgaa
    14641 gtggtgcgtg atgaaattac agaattgcca acagatgtta ttacagttgt ggcgaccggt
    14701 cccttgacta gcgatgccct ggctgaaaag attcatgccc ttaatgatgg cgatggcttc
    14761 tatttctacg atgcagcagc gcctattatc gatgtcaaca ctatcgatat gagtaaggtc
    14821 tatctcaaat ctcgttatga taagggagaa gcagcctatc tcaatgctcc aatgaccaag
    14881 caagagttta tggatttcca tgaagccttg gtcaatgcgg aagaagcacc gctcaattct
    14941 tttgaaaaag aaaagtactt tgaaggatgt atgccaatcg aagttatggc caaacgtggc
    15001 attaaaacta tgctttatgg ccctatgaag ccagtcggtc ttgagtatcc ggacgactac
    15061 acaggacctc gtgatggaga atttaaaaca ccttatgcgg ttgtgcaact tcgtcaggat
    15121 aatgcagctg gtagcctcta caatatcgtt ggtttccaga cccacctcaa atggggagaa
    15181 caaaagcgtg tcttccaaat gattccgggt cttgaaaatg cggagtttgt ccgttatggt
    15241 gtcatgcatc gcaattctta catggattca ccaaatcttc ttgagcagac ttaccgttcc
    15301 aagaaacaac caaatctctt ctttgctggt cagatgacgg gggtggaagg ctatgttgag
    15361 tcggcggctt cgggcttagt tgcgggaatt aacgcagctc gtctcttcaa ggaagaaagc
    15421 gaggttattt tccccgagac gacagcgatt ggaagcttag ctcattacat tacccatgcc
    15481 gacagcaaac atttccaacc aatgaatgtc aattttggga tcatcaagga gttggaaggc
    15541 gagcgtatcc gtgataagaa ggctcgttat gaaaaaattg cagagcgtgc ccttgccgac
    15601 ttagaggaat ttttgactgt ctaatttttt tgaaagaatt gctcatgata ctataaaaat
    15661 cttagaaatt gtgataaaat aggtaggatg aaagaaggag agtgaaaatg gcgaatccca
    15721 agtataaacg tattttaatc aagttatcag gtgaagccct tgccggtgaa cgtggcgtag
    15781 ggattgatat ccaaacagtt caaacaatcg caaaagagat tcaagaagtt catagcttag
    15841 gtatcgaaat tgcccttgtt attggtggag gaaatctctg gcgtggagaa cctgcagcag
    15901 aagcaggtat ggaccgtgtt caggcagatt acactggaat gcttgggact gttatgaatg
    15961 ctcttgtgat ggcagattca ttgcaacaag ttggggttga tacgcgtgta caaacagcta
    16021 ttgctatgca acaagtggca gagccttatg tccgtggacg tgcccttcgt caccttgaaa
    16081 aaggccgtat cgttatcttt ggtgctggaa ttggttcacc atacttctcg acagatacaa
    16141 cagcggccct tcgcgcagct gaaatcgaag cagatgccat cctcatggct aaaaatggcg
    16201 tcgatggtgt gtacaatgcc gatcctaaga aggacaagac agccgttaag tttgaagaat
    16261 tgacccaccg tgatgttatc aacaaaggtc ttcgtatcat ggactcaaca gcctcaaccc
    16321 tctcaatgga caacgacatt gacttggttg tcttcaacat gaaccaatca ggcaacatca
    16381 aacgtgtcgt atttggtgaa aatatcggaa caacagtttc aaataatatc gaagaaaagg
    16441 aataagaaag aatatggcta acgtaattat tgaaaaagct aaagagagaa tgacccagtc
    16501 tcaccaatca cttgctcgtg aatttggtgg tatccgtgct ggtcgtgcca atgcaagctt
    16561 gcttgaccgt gtacatgtag aatactatgg agtcgaaact cctcttaacc aaatcgcttc
    16621 aattacgatt ccagaagcgc gtgttttgtt ggtaacacca tttgacaagt cttcattgaa
    16681 agacatcgaa cgtgccttga acgcttctga tcttggtatc acaccggcta atgacggttc
    16741 tgtgattcgc ttggttatcc cagctcttac agaagaaact cgtcgtgacc ttgctaaaga
    16801 agtgaagaag gtcggcgaaa atgctaaagt ggctgtccgc aatatccgtc gcgatgctat
    16861 ggacgaagct aagaaacaag aaaaagcaaa agaaatcact gaagacgaat tgaagactct
    16921 tgaaaaagat attcaaaaag taacagacga tgctgttaaa cacatcgacg acatgactgc
    16981 taacaaagag aaagaacttt tggaagtcta aaaataaaca gaaaaactca gttggcattg
    17041 ctggctgagt tttattcgaa agaaggaaat atgaatacaa atcttgcaag ttttatcgtt
    17101 ggactgatca tcgatgaaaa tgaccgtttt tactttgtgc aaaaggatgg tcaaacctat
    17161 gctcttgcta aggaagaggg ccaacataca gtaggggata cggtcaaagg ttttgcatac
    17221 acggatatga agcaaaaact ccgcctgaca accttagaag tgactgccac tcaggaccaa
    17281 tttggttggg gacgtgtcac agaggttcgt aaggacttgg gtgtctttgt ggatacaggc
    17341 cttcctgaca aggaaatcgt tgtgtcactc gatattctcc ctgagctcaa ggaactctgg
    17401 cctaagaagg gcgaccaact ctacatccgt cttgaagtgg ataagaaaga ccgtatctgg
    17461 ggcctcttgg cttatcaaga agacttccaa cgtcttgctc gtcctgctta caacaacatg
    17521 cagaaccaaa actggccagc cattgtttac cgtctcaagc tgtcaggaac ttttgtttac
    17581 ctaccagaaa ataatatgct tggttttatt catcctagcg agcgttacgc agagccacgt
    17641 ttggggcaag tattagatgc gcgcgttatt ggtttccgtg aagtggaccg cactctgaac
    17701 ctctccctca aaccacgctc ctttgaaatg ttggaaaacg atgctcagat gattttgact
    17761 tatttggaaa gcaatggcgg tttcatgacc ttaaatgaca agtcatctcc agacgacatc
    17821 aaggcaacct ttggcatttc taaaggtcag ttcaagaaag ctttaggtgg tcttatgaag
    17881 gctggtaaaa tcaagcaaga ccagtttggg acagagttga tttagggagg cttatgagaa
    17941 aatcatttta catttggctc atgaccgagc gcaatcctaa aagtaacagt cccaaagcaa
    18001 ttttggcaga cctcgctttt gaagagtcag cctttccaaa acatacagat gattttgatg
    18061 aggtcagtcg ctttttggag gagcatgcca gtttctcttt taacctagga gattttgaca
    18121 gcatttggca ggaatatcta gaacactagc atttattcat tgggtttggg ctagtaattt
    18181 ctccatccct ctgctataat aaaaagaaat aaaaggatta gagaggttct ttatttgaag
    18241 gaacattcaa tagacattca actgagtcat ccagatgacc tgtttcatct ttttggttcc
    18301 aatgaacgcc atcttcgttt gatggaagaa gagcttgatg ttgtgattca tgctcgtacg
    18361 gagattgtcc aggttttggg agaagagtct gcttgcgagg aagcccgtca ggttattcaa
    18421 gctctcatgg ccttggtaaa tcgtgggatg accgttggta cgccagatgt agtgacggct
    18481 attagtatgg tcaaaaatga tgaaattgac aagtttgtcg ccctttacga agaagaaatc
    18541 atcaaggata atactgggaa gcctatccgc gtcaaaacct taggtcaaaa actttatgtg
    18601 gacagtgtca aacagcatga tgtgaccttt ggaattgggc cggcaggtac agggaagacc
    18661 ttccttgcag tgaccttggc agtgactgcc cttaaacgtg ggcaagtcaa gcgaattatc
    18721 ctaactcgtc cagcggtgga agcgggagag agtcttggat ttcttccggg tgatcttaag
    18781 gagaaggtgg atccttacct tcgtcctgtt tacgatgcct tgtatcaaat tcttgggaaa
    18841 gaccaaacga ctcgtctcat ggagcgtgaa attatcgaaa ttgcgcccct tgcctatatg
    18901 cgtggccgga ccttggatga tgcctttgtc attctcgatg aggcgcaaaa cacgaccatc
    18961 atgcagatga agatgttctt gacgcgttta ggctttcatt ctaagatgat tgtcaatgga
    19021 gatatcagtc agattgacct gccacgtaat gtcaagtccg gtttgattga tgctcaagag
    19081 aaactcaaga acatccatca gattgacttt gttcattttt cagctaagga tgtggttcgc
    19141 catccagttg tcgctcaaat tatccgagcc tatgaatatt ctactgaagt tgcacacgac
    19201 tgattttgag gaagttcgcc tgcaaaagaa tatacttttg agagaggaaa atccagttgt
    19261 ataggctaaa ggttttatcc aaaggtttga gacaacgatt aggcacgatg gaaagaactt
    19321 ttatgtggct gatgacgatc agtgcatctt cctgtgtcat aatcacaggg aacaagaaag
    19381 taggaatttg aaaagatgat tgaccaacta tctaagtatt acagttgtag gatactaact
    19441 gaaaaggata ttccaagtat tttatcttta tatgaaagta atcctctgta ttttcagcat
    19501 tgtccaccag agccaaattt tgcaactgta aaagaggaca tgctttgtct acctgaaggt
    19561 aaagctaagg ctgataagtt ttttgttgga ttttggaatg gctctgacct tgtggctgtt
    19621 atggattttg tctatgcata tcctgatgag gagactgttt ttattggttt gtttatggtt
    19681 gatcaagcct atcagaggaa agggattggt agtcatattg tgacagaagc actagcttat
    19741 tttgctaaga actttcgaaa ggcacgtttg gcttatgtta agggaaatcc gcaatctcag
    19801 catttttggg aaaagcaggg ctttaaatca attggatgcg aggttaagca agaactctat
    19861 acggttgtta tcgttgaaca gagcctagaa gattagaaat ggcatcaagt aagaactatt
    19921 tggaatttgt tttggaacaa ttatcaggat tagatgatgt gacttaccgt tccatgatgg
    19981 gggagtatat tctttacttc cgcggcaaga ttattggcgg tatttatgac gatcgctttt
    20041 agttaaaccc gtgcaagcag tcttagataa gattgaccaa tcttcttttg agtttccata
    20101 caaaggtgcc aaagaaatga tttgagtgga agaacttgat aataagatgt ttctataaga
    20161 cctgatttta gctatgtata accaactgcc aacgcccaaa cctaaaaaga aaaagcaagg
    20221 gtgaacgaag taaaaaagaa gtctgctaag gccctgtctt tgcacgggta aaattttata
    20281 tataaaaaga agctgggact aaagagctca gcttcctttg gtttatataa ttgtcattac
    20341 aagacgaagt ggttgggcga aactctgttg actttattca atttagagtt tcttatgcac
    20401 aattgagtct ggaacgaaag tctccagttg caaagtatac agtacaataa accaacgatg
    20461 taatagctga tgacacaaag cacagtgggt aggacttgcg aagtcaccct tttcttttca
    20521 aaatttatac taaatcattg atatcagtgt agtcacgatt aagtccttga gcaactggta
    20581 ggctagtcaa gtaaccttga taagtggtca caccttgacg caagccttca tcttcagaga
    20641 ttgcttgtgc gaatcctttg ccagccaaag cttcgatata aggaagagtg acattggtta
    20701 gggcgatggt tgaagtgcga gcaaccgcac cagggatatt ggcaacggca tagtggagaa
    20761 caccgtgttt ttcatagacg ggttcatcgt gcgttgtcac acggtcagct gtttcgataa
    20821 ggccaccttg gtcaacagca aggtcaacga tacagagcct ggacgcattt gtttgaccat
    20881 ctcatctgtc accaattccg gtgcttttgc accagggatg agaatggctc caatcaccac
    20941 atcagcatct ctcacacttg cttcaatgtt gaatgaatta gatataagag tttaaatttg
    21001 acttccaaag acttctttta gaactgagag acgcttggca ctaatatcta aaatagtcac
    21061 ttgagcacca agaccaaggg cgatgcgggc agcatgtgta ctgacgacac catcaccgat
    21121 gatagttact tttccttttg gaacacctgg tacaccacca ggtagaacac cagagccacc
    21181 agcttgctta gtaaggaagt gagctccgat ttaaacagcc atacgatctg caacctcact
    21241 cataggaacg aggagcggta gttgtccttg attgtcacga acagtttcat aggcaattcc
    21301 tgttgttttt gctgctaaca tagcatctgc taattctgga gcagcggcca tgtgcaagta
    21361 ggtgaagaga agaagatcgt cgcgaaagta accgtattca gaacttaaag gttcttttac
    21421 tttcacaacc aactctgctg cccaagcttc accagcagta gcgacaatct cagctccttg
    21481 cttttgatag tcagcatcag taaagccaga accgagacca gcatttgttt cgataaggac
    21541 acgatgacca cgactaacta agctatgaac acctgcaggt gtgagggcga cacggttttc
    21601 gttattttta atttcttttg ggattccgat taacattgag ataacctacc tttcaattga
    21661 cggtcttgtt ttggttgtca cattccagtt cataaatcaa aaatgtgacg gtttcattgt
    21721 atatgaaacc gcttcaaaaa tcaagaaaaa cttgtcatcc aaatttttta tgctagacta
    21781 gtgaaaatca agctttgatt ggttggaaaa gtatggaatc aatttttgta aaacttgccc
    21841 agtatccgtc tatagaaacg gagcgtttat tgcttagacc tgtaactttg gatgatgcgg
    21901 aagcaatgtt tgactatgcc tcggacaagg gtaatacacg ttacactttt ccaaccaatc
    21961 aaagtttgga agaaaccaag aataacattg ctcagttcta tttggctaat cccttgggac
    22021 gttggggaat agaactaaaa agcaatggtc agtttattgg aaccattgac ttgcacaaga
    22081 ttgattctgt tcttaagaag gcagctattg gctacattat caataaaaag tattggaatc
    22141 aaggattaac gacagaagcc aatcgtgctg tgattgagct agcttttgag aagataggga
    22201 tgaataagtt gactgccctt cacgataagg acaatcctgc gtcaggaaag gtcatggaga
    22261 aatcaggcat gcgtttttcc catgcagaac catatgcttg tatggaccag catgaaaaag
    22321 gccgaatcgt gacaagagtt cattatgtct tgaccaagga agactatttt gcaaataaat
    22381 aagcagttga aaagaaattt ttcgactgtt ttttcttcct cttacgaata atctaagaga
    22441 ggagaaaata tggaagcaat tatcgagaaa atcaaagagt ataaaatcat cgtcatctgt
    22501 actggtctgg gcttgcttgt aggaggattt ttcctgctaa aaccagctcc acaaacacct
    22561 gtcaaagaga cgaatttgca ggctgaagtc gcagctgttt ccaaggattt ggtatccgaa
    22621 aaggaagtga acaaggaaga aaaggaagaa ccccttgaac aagatctaat cacagtagat
    22681 gtcaaaggtg ctgtcaaatc gccagggatt tatgacttgc ctgtaggtag tcgagtcaat
    22741 gatgctgttc agaaggctgg tggcttgaca gagcaagcag acagcaagtc gctcaatcta
    22801 gctcagaaag ttagtgatga ggctctggtt tacgttccta ctaagggaga agaagcagtt
    22861 agccaacaga ctggtttggg gacagcttct tcaataagca aggaaaagaa ggtcaatctc
    22921 aacaaggcca gtctggaaga actcaagcag gtcaagggac tgggaggaaa acgagctcag
    22981 gacattatcg accatcgtga ggcaaatggc aagttcaagt cagtagacga gctcaagaag
    23041 gtctctggca ttggtggcaa aacaatagaa aagcttaaag actatgttac agtggattaa
    23101 gaatttctct attcccctaa tttacctgag ttttctgtta ctttggcttt actacgctat
    23161 tttctcagca tcctatcttg ctttgttggg atttgttttt ctgctagtct gtctcttttt
    23221 ccaatttcct tggaaatctg ctagcaaagt tctagtgatt tgtggaatct ttggattttg
    23281 gtttgttttt caaaattggc aacagagtca agcgagtcaa aatctggcgg attctgttga
    23341 aagggtacgg attttgcctg atactattaa ggtcaatggt gatagtctat cctttcgtgg
    23401 caagtctaac ggtcgtgctt tccaagtcta ttataaactc cagtccgagg aggagaaaga
    23461 agcctttcaa gctttaaccg acctgcatga gataggacta gaagggaagc tttcggagcc
    23521 agaagggcag agaaattttg gtggctttaa ttaccaagcc tatctgaaga ctcagggaat
    23581 ttaccagaca ctcaatatta aaagaatcca gtcactccaa aaggttggca gttgggatat
    23641 aggtgaaaaa ctgtccagtt tacgtcgaaa ggctgtggtt tggattaaga tgcacttccc
    23701 agaccctatg cgcaattaca tgacaggact cttgctagga catctggaca ccgattttga
    23761 ggagatgaat gagctttatt ccagtttagg aattattcac ctttttgcct tgtcaggtat
    23821 gcaggtaggg tttttcatga atggatttaa gaaacttctc ttgcgattgg gcttgaccca
    23881 agaaaagttg aaatggctga cttatccctt ttcccttatc tatgcgggac taactggatt
    23941 ttcagcatcg gttattcgca gtctcttgca aaagctactg gctcaacatg gggttaaggg
    24001 cttggataat tttgccttga cggtgcttgt cctctttatt gtcatgccaa actttttctt
    24061 gacagcagga ggagtcttgt cctgcgctta tgcttttatc ctgaccatga ccattaaaga
    24121 agggaagggg ctcaaggctg ttgctagtga aagtctagtc atctccttgg gcatattgcc
    24181 cattctatcc ttctattttg cggaatttca accttggtct atccttttga cctttgtctt
    24241 ttcctttcta tttgacttga ccttcttacc gctcctgtct attttatttg tcctttcctt
    24301 tctctatcca gtcattcagc tgaactttat ctttgaatgg ttggagggca ttattcgctt
    24361 ggtctcacag gtgacaagta ggcctctagt ctttggacaa cccaatgaat ggcttttaat
    24421 cctattgtta atttctttgg ctttggtcta tgatttgagg aaaaacatta aaggattaac
    24481 agtattgagt ttattgatta caggtctctt tttcttgacc aagtatccac tggaaaatga
    24541 aatcaccatg ctggatgtgg ggcaaggaga aagtattttc ctacgggatg taactgggaa
    24601 aaccattctc atagatgtag gtggtaaggc agaatcttat aagaaaatcg aaaaatggca
    24661 agaaaagatg acgaccagca atgcccagcg aaccttgatt ccctatctca aaagtcgagg
    24721 agtagctaag attgaccagc taattttgac taacacggac aaggagcatg ttggagattt
    24781 gtcagaggtg accaaggctt tccatgtagg ggagatttta gtatcaaaag gcagtttgaa
    24841 gcagaagcaa tttgtggtag aactacaggc gactaaaaca aaggtgcgta gtatgacagt
    24901 aggggagaac ttgcccattt ttggaagtca gttagaagtt ctatctccaa ggaaaatggg
    24961 agatggagga cacgatgata ccctagttct gtatgggaaa ttcttggata agcaatttct
    25021 cttcacggga aatttggagg agaaaggaga gaaggacttg ctgaagcact atccagactt
    25081 gaaagtaaat gttttgaaag ctagccaaca tggcaataaa aaatcatcaa gcccagcctt
    25141 tctagaaaaa ctcaaaccag agcttactct tatctcagtt ggaaagagca atcgaatgaa
    25201 actcccccat caggaaacat tgacacgact ggaaggtatc aatagcaaag tttatcgaac
    25261 tgaccagcaa ggagctatac gttttaaggg gttggatagt tggaaaatcg aaagtgttcg
    25321 ataggaaaga taaatgttgt agattagtga aataaactaa aaatttgttg cataataatg
    25381 ataaaaatgg tataatgaaa acgtattcaa tattgaggat ataaaatcat taaaaatcag
    25441 caaaagttat tttattagtt agtttataat ctattggtct tcttcagtcc agtgtatctg
    25501 ctaagagtgt tcttaaaagt atcggtatga ttggaatatc ttctataagt ctagtatgaa
    25561 tttccatgga tatagacata ctaacgttcc gtcatggtct cgttattaca gctattctga
    25621 gtatccagtt ggttctggct ggaactacgg tcgttatgag atcataaact attacagcgg
    25681 aggctattaa ttcttaaaga gtgagaaaaa ggagggctag atatgttgca gcttactcat
    25741 gtgaccttaa aaacgcgaca agtcatcttg caagatgcgg atttcacctt taaaaagggt
    25801 aggttttatg gtcttcttgc tatcaatggc tctggaaaga cgaccctgtt ccgtgccatt
    25861 agcaatttaa ttcccataag tagtggaaat atcgcagccc ctccttcttt attttattat
    25921 gagagtattg aatggctgga tggaaactta agtgggatgg actaccttcg tcttatcaaa
    25981 aacatctgga agtcagacct aaacttgaga gatgaaatcg cctactggga aatggctgac
    26041 tatatcagtc ttcccatccg caagtattcc ttaggcatga agcaacgctt ggtgattgct
    26101 atgtattttc tcagtcaggc aaaatgctgg ctcatggatg agattacaaa tggcttagac
    26161 gagtattatc gacagaagtt ttttgatagg ctagcacaaa tcgatagaca agaacagctg
    26221 gttcttttaa gttcccacta taaggaagag ttggttgata tctgcgatag agtagtaacc
    26281 attcatcagg ggcagataga agaggtttag tttatgaaag atgttagtct atttttattg
    26341 aaaaaagttt tcaaaagtcg tttaaactgg attatcttac ttttatttgc atctgtactc
    26401 ggtgttacct tttatttaaa tagtcagact gcaaactcac acagcttgga gagcaggttg
    26461 gaaagtcgca ttgcagccaa cgagagagcc atcaatgaaa atgaagcgaa actttcccaa
    26521 atgtctgaca ccagctcgga ggaataccag tttgctaaaa gtaatttaga attgcaaaaa
    26581 aatcttttga agcgaaagac agaaattctg actttattaa aagaagggcg ctggaaagag
    26641 gcctactatc tgcagtggca agatgaagag aagaattatg aatttgtatc aaatgacccg
    26701 actgctagct ctggcttaaa aatgggggtt gaccgcgaac ggaagattta ccaagccctg
    26761 tatcccttga acataaaagc acatactttg gagtttccga cccacgggat tgatcagatt
    26821 atctggattt tagaggctat catcccaagc ttgtttgtgg ttgctattat ttttatgcta
    26881 acacaactat ttgcagaaag atatcaaaat catctggaca cagctcactt atatcctgtt
    26941 tcaaaagtga catttgcaat gtcttctctt ggagttggag tgggctatgt aactgtgctg
    27001 tttatcggaa tctgtggctt ttcttttcta gtgggaagtc tgataagtgg ttttggacag
    27061 ttagattatc cctacccgat ttatagctta gtgaaacaag aggtaactat tggaaagatg
    27121 caggatgtat tatttcctag cttgctctta gctttcttag cctttatcgt cattgtggaa
    27181 gttgtgtatt tgattgctta ctttttcaag caaaaaatgc ctgtcctctt tctttcactc
    27241 attgggattg ttggcttatt gtttggcatc caaaccattc agcctcttca aaagattgca
    27301 catctgattc cctttactta cttgcgttca gtggagattt tatctggaag attacctaag
    27361 cagattgata atgtcgatct aaattggagc atgggaatgg tcttacttcc ttgcctgatt
    27421 atctttttgc tattgggaat tctatttatt gaaagatggg gaagttcaca gaaaaaaaga
    27481 attttttaat agattctagc tttcctatag gtagggaaaa taagtaaaaa ctaacataga
    27541 gagggaatca acttgattct ctctttttga tttgaaaacc aaaccaaaat acaaacacaa
    27601 acttttcaaa aaataacttt ttatcttgac aagagctaga aaacttggta tcatataaaa
    27661 gttgagaaaa gcagaagtga gagcttctcg ccttgtgaca ttaagttgcc tggccctacg
    27721 gatgaaaagt ttcgaagaaa cgctatcata acgtgcgggc ttgtatattt acgagtccgc
    27781 tattgttttt ctctaataaa acaaaagagg tgaaaaccat agcaaagcaa gacttattca
    27841 tcaatgatga gattcgtgta cgtgaagttc gcttgattgg tcttgaagga gaacagctag
    27901 gcatcaagcc actcagtgaa gcgcaagctt tggctgataa cgctaatgtt gacctagtat
    27961 tgattcaacc ccaagccaaa ccgcctgttg caaaaattat ggactacggt aagttcaaat
    28021 ttgagtacca gaagaagcaa aaagaacaac gtaaaaaaca aagcgttgtt actgtgaaag
    28081 aagttcgtct aagtccaact attgacaagg gtgactttga tacaaaactt cgtaatgcac
    28141 gtaagttcct tgaaaaagga aataaagtta aggtatctat tcgctttaag ggtcgtatga
    28201 ttacccataa agagattggt gcaaaagttt tagccgagtt tgctgaagca actcaagata
    28261 ttgccatcat cgaacaacgt gctaaaatgg atggacgtca aatgttcatg caattggcgc
    28321 cagcgactga caaaaaataa ttgtcagaaa gttaaataaa ggagaaaaaa tcatgccaaa
    28381 acaaaaaaca caccgcgcat cagctaaacg tttcaaacgt acaggttctg gtggacttaa
    28441 acgtttccgt gcttacactt ctcaccgttt ccacggaaaa actaagaaac aacgtcgtca
    28501 tcttcgtaaa gcatctatgg tgcattcagg agattacaaa cgtatcaaag caatgcttac
    28561 tcgcttgaaa taatagcttg actgtaagta aacaattcta ggaaatattt ggaggaaata
    28621 aaacatggca cgtgttaaag gtggcgttgt atcacgcaaa cgtcgtaaac gtattcttaa
    28681 attagcaaaa ggttactatg gagctaaaca catcttattc cgtactgcaa aagaacaagt
    28741 aatgaactct tactactatg cataccgtga ccgtcgtcag aaaaaacgtg acttccgcaa
    28801 attgtggatt actcgtatca atgcggcagc tcgtatgaac ggactttcat actcacaatt
    28861 gatgcatggt ttgaaattgg ctgagattga agttaaccgt aaaatgcttg ctgacttggc
    28921 tgttaacgat gcagtagctt tcacagctct tgcagatgca gctaaagcaa aacttggtaa
    28981 ataacaactg atgagactgg aacaggattg tccagtcttt ttaaaaataa aggagaaaga
    29041 tatggcttca aaaatgctac acacttgctt acgagtagaa aatcttgaaa aatcaatcgc
    29101 attttatcaa gatgcttttg gttttaaaga attgcgtcgc agagattttc cagaccatgc
    29161 cttcacgatt gtctatctag gtcttgaggg tgatgattat gagttggagt tgacttataa
    29221 ctacgatcac ggtccgtatg tggttggtga tgggtttgcc catatcgctc tcagtacacc
    29281 tgatcttgag gcacttcatc aagaacacag tgcaaaaggc tatgaagtga ctgagccaaa
    29341 tggtctacca ggaactacac caaactatta ctttgtcaaa gaccctgatg gatataaggt
    29401 cgaagtcatt cgtgaaaaat aatttcgtgc aagttaagta gaatattcgt tttctacttg
    29461 acttttttta gctgaaagag tatactaata aaaattcaac ctttaagggg agtccagaga
    29521 gactcacaag gtgtcagata aaagaatagt gcaattttgt agaggagacc ttttgagtgt
    29581 gctctcttgt gttgtacgat tttaactgag gccttgcact agcaaggtct tttctttatc
    29641 tggtcccctt aaaatttaag gaggaaaagt tatgaatctc acatgtaaga agcgtttggg
    29701 tgtcattcgg ttggaaacca tgaaggtggt tgcacaagag gaaatcgcgc cagcaatctt
    29761 tgaattagtc ctagaaggag aaatggttga agccatgcga gcaggccaat ttcttcatct
    29821 gcgtgtacca gacgatgccc atctcttacg tcgtcctatt tcaatttcgt ctattgacaa
    29881 ggcaaacaag cagtgtcacc tcatttatcg gattgaggga gctgggactg caattttttc
    29941 aaccttaagt cagggagaca ctcttgatgt gatggggcct cagggaaatg gttttgactt
    30001 gtctgacctt gatgagcaga atcaggttct ccttgttggt ggtgggattg gtgttccacc
    30061 cttgcttgag gtggccaagg aattgcatga acgtggagtg aaagtagtga cagtcctcgg
    30121 ttttgctaat aaggatgctg ttattttgaa aacggaattg gctcagtatg gtcatgtctt
    30181 tgtaacgaca gatgatggtt cttatggcat caagggaaat gtttccgttg ttatcaatga
    30241 tttagacagt cagtttgatg ctgtttactc gtgtggggct ccaggaatga tgaagtatat
    30301 caatcaaacc tttgatgatc acccaagagc ctatttatct ctggaatctc gtatggcttg
    30361 tgggatggga gcttgctatg cctgtgttct aaaagtacca gaaaacgaga cggtcagcca
    30421 acgcgtctgt gaagatggtc ctgttttccg cacaggaaca gttgtattat aaggagaaaa
    30481 ttatgactac aaatcgatta caagtttctc tacctggttt ggatttgaaa aatccgatta
    30541 ttccagcatc aggctgtttt ggctttggac aagagtatgc caagtactat gatttagacc
    30601 ttttaggttc tattatgatc aaggcgacaa cccttgaacc acgttttggg aatccaactc
    30661 caagagtggc agagacgcct gctggtatgc tcaatgcaat tggcttgcaa aatcctggtt
    30721 tagaggttgt tttggctgaa aagctacctt ggctggaaag agaatatcca aatcttccta
    30781 ttattgccaa tgtagctggt ttttcaaaac aagagtatgc agctgtttct catgggattt
    30841 ccaaggcaac taatgtaaaa gctatcgagc tcaatatttc ttgtcccaat gttgaccact
    30901 gtaatcatgg acttttgatt ggtcaagatc cagatttggc ttatgatgtg gtgaaagcag
    30961 ctgtggaagc ctcagaagtg ccagtttatg tcaaattaac cccgagtgtg accgatatcg
    31021 ttactgtcgc aaaagctgca gaagatgcgg gagcaagtgg cttgaccatg atcaatactc
    31081 tggttggaat gcgctttgac ctcaaaacca gaaaaccaat cttggccaat ggaacaggtg
    31141 gaatgtcagg tccagcagtc tttccagtag ccctcaaact catccgccaa gtagcccaaa
    31201 caacagacct gcctatcatt ggaatggggg gagtggattc gactgaagct gccctagaaa
    31261 tgtatctggc tggggcatct gctatcggag ttggaacagc taactttacc aatccttatg
    31321 cctgccctga catcatcgaa aatttaccaa aagtcatgga taaatacggt attagcagtc
    31381 tggaagaact ccgtcaggaa gtaaaagagt ctctgaggta aactgcaatc aatctgttct
    31441 tgatttttta ttagtttgta atatgaattt aggagaattt tggtacaata aaataaataa
    31501 gaacagagga agaaggttaa tgaagaaagt aagatttatt tttttagctc tgctattttt
    31561 cttagctagt ccagagggtg caatggctag tgatggtact tggcaaggaa aacagtatct
    31621 gaaagaagat ggcagtcaag cagcgaatga gtgggttttt gatactcatt atcaatcttg
    31681 gttctatata aaagcagatg ctaactatgc tgaaaatgaa tggctaaagc aaggtgacga
    31741 ctatttttac ctcaaatctg gtggctatat ggccaaatca gaatgggtag aagacaaggg
    31801 agccttttat tatcttgacc aagatggaaa gatgaaaaga aatgcttggg taggaacttc
    31861 ctatgttggt gcaacaggtg ccaaagtaat agaagactgg gtctatgatt ctcaatacga
    31921 tgcttggttt tatatcaaag cagatggaca gcacgcagag aaagaatggc tccaaattaa
    31981 agggaaggac tattatttca aatccggtgg ttatctactg acaagtcagt ggattaatca
    32041 agcttatgtg aatgctagtg gtgccaaagt acagcaaggt tggctttttg acaaacaata
    32101 ccaatcttgg ttttacatca aagaaaatgg aaactatgct gataaagaat ggattttcga
    32161 gaatggtcac tattattatc taaaatccgg tggctacatg gcagccaatg aatggatttg
    32221 ggataaggaa tcttggtttt atctcaaatt tgatgggaaa atagctgaaa aagaatgggt
    32281 ctacgattct catagtcaag cttggtacta cttcaaatcc ggtggctaca tggcagccaa
    32341 tgaatggatt tgggataagg aatcttggtt ttacctcaag tttgatggca aaatggctga
    32401 aaaagaatgg gtttacgatt ctcatagtca agcctggtac tacttcaaat ccggtggtta
    32461 catgacagcc aatgaatgga tttgggataa ggaatcttgg ttttacctca aatctgatgg
    32521 gaaaatagct gaaaaagaat gggtctacga ttctcatagt caagcttggt actacttcaa
    32581 atccggtggt tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttacct
    32641 caagtctgat ggcaaaatgg ctgaaaaaga atgggtctac gattctcata gtcaagcctg
    32701 gtactacttc aaatctggtg gctacatggc gaaaaatgag acagtagatg gttatcagct
    32761 tggaagcgat ggtaaatggc ttggaggaaa agctacaaat gaaaatgctg cttactatca
    32821 agtagtgcct gttacagcca atgtttatga ttcagatggt gaaaagcttt cctatatatc
    32881 gcaaggtagt gtcgtatggc tagataagga tagaaaaagt gatgacaagc gcttggctat
    32941 tactatttct ggtttgtcag gctatatgaa aacagaagat ttacaagcgc tagatgctag
    33001 taaggacttt atcccttatt atgagagtga tggccaccgt ttttatcact atgtggctca
    33061 gaatgctagt atcccagtag cttctcatct ttctgatatg gaagtaggca agaaatatta
    33121 ttcggcagat ggcctgcatt ttgatggttt taagcttgag aatcccttcc ttttcaaaga
    33181 tttaacagag gctacaaact acagtgctga agaattggat aaggtattta gtttgctaaa
    33241 cattaacaat agccttttgg agaacaaggg cgctactttt aaggaagccg aagaacatta
    33301 ccatatcaat gctctttatc tccttgccca tagtgcccta gaaagtaact ggggaagaag
    33361 taaaattgcc aaagataaga ataatttctt tggaattaca gcctatgata cgacccctta
    33421 cctttctgct aagacatttg atgatgtgga taagggaatt ttaggtgcaa ccaagtggat
    33481 taaggaaaat tatatcgata ggggaagaac tttccttgga aacaaggctt ctggtatgaa
    33541 tgtggaatat gcttcagacc cttattgggg cgaaaaaatt gctagtgtga tgatgaaaat
    33601 caatgagaag ctaggtggca aagattagta ctataagtga atatgatttg agtgaatagt
    33661 aagttaaaaa tcctgatttc aagtaaaatc aggatttttt catggatgca atttttttgg
    33721 agtctggtgt gacgcggagg gtcttttgtc ctgtgtaagt gacaaagccg ggttttccac
    33781 cagttggttt attgagtttt ttgacttcaa tcatatctac ctgcaccaga ttcgacaggc
    33841 gcccttgaga gaagtaggca gctaactctg ctgcgtctgt cttgactgca tcagatgggt
    33901 caagatttcc tgagatgaca acatggcttc caggaatgtc cttagcatgg aaccaaagtt
    33961 cctccttgcg ggccatttta aaggtcaatt cctcattttg aagattgttt cgtccgacat
    34021 agatgatggt tttgccatcg cttgctagat attgttctag ttttttgcgt ttctggattt
    34081 tctcccgttg tcttctgcgg ataaaacctg tttgaatcaa ttcttcacgg atttcagcga
    34141 tttcttccag tccagcttgg ttgaggacgg tttctacact ttccagatag agaatagtgg
    34201 ctttggtttc ttcaatcaaa tcagtcaagt atttgacagc ttctttgagt ttctgatacc
    34261 gtttaaaata gcgttgggca ttctggttgg gagtcagagc cttatcaagc gcaatcatga
    34321 taggttggtt ggtatagtag ttgtctagga taacctggtc ttggtcgtta ggtacttggt
    34381 ggaggaaggt tgtcagcaat tctccttttt gacgaaattc ttcagcgttg tctgtctcca
    34441 gtaactcttt ttcctgtttt ttgagtttgt gtcggttttt ctgaagttca ttttcaacac
    34501 gacgaatcag ttcactggcc tgctgtttga cgcggtcgcg ctcagcctta tccttatagt
    34561 aggtgtccaa caaatcagaa agatttgcaa aaggctctcc cacctgattt gcaaaaggaa
    34621 ctggactgaa ggaagtctca gtcaagcatg gcttggtttc ttgattgaaa aaatttcgga
    34681 aagcggaaag tttttcacta accagtatcc tttccaattc atttgcagta tcgcgtccca
    34741 gaccttgaaa gaggctttga agattttttg ctgttagttc ttgggtttgc aggatttcaa
    34801 agagcttttc atctttgata gtaaaaggat tgagagattc tgtacttggc ggagcgatat
    34861 aggtcgatcc tggaagtaag gtgcggtagc tattttgtga aaaaccgacg tgtttgataa
    34921 cttcgaggat tttatgactg cttttatcga ccagtagaat attactgtgt ttccccataa
    34981 tttcaataat caaggtagcc tggatatggt ctccaatctc gtttttattg gaaactgtaa
    35041 tttccacaat acggtcattt tccacttgct caatcgactc aatcagggcc ccctgcaaat
    35101 actttctcaa aaccatgata aaggtagaag gttgagctgg attttcaaaa gtcgtttggg
    35161 tcagctgaat gcgtccaaaa actggatggg cagaaaggag caggcgatgg ctttggcgat
    35221 tgctgcggat ttgcaagacc aactcttgtt caaaaggctg attgattttc tggatgcgac
    35281 cattcactaa ttcgcttcgc aattcctcaa ctatgtggtg taaaaaaaat ccgtcaaatg
    35341 acatcgttct ctccttgtga ttgtattcca tagtattata tcaaaaaggt agaataaaat
    35401 catggaaatg tggtataata aagccaagta aagagaaacg agaagcacat gtatattgaa
    35461 atggtagatg aaactggtca agtttcaaaa gaaatgttgc aacaaaccca agaaattttg
    35521 gaatttgcag cccaaaaatt aggaaaagaa gacaaggaga tggcagtcac ttttgtgacc
    35581 aatgagcgta gtcatgaact taatctggag taccgtgaca ccgaccgtcc gacagatgtc
    35641 atcagccttg agtataaacc agaattggaa attgcctttg acgaagagga tttgcttgaa
    35701 aatccagaat tggcagagat gatgtctgag tttgatgcct atattgggga attgttcatc
    35761 tctatcgata aggctcatga gcaggccgaa gaatatggtc acagctttga gcgtgagatg
    35821 ggcttcttgg cagtacacgg ctttttacat attaacggct atgatcacta tactccggaa
    35881 gaagaagcgg agatgttcgg tttacaagaa gaaattttga cagcctatgg actcacaaga
    35941 caataaacga aaatggaaaa atcgtgacct gatatccagt ttagaatttg ccatcacagg
    36001 gatttttact gctatcaagg aagaacgcaa tatgcgaaaa catgcagtga cggctctagt
    36061 ggtcatcctt gcaggttttg gttttcaggt gtcacgaatc gaatggctct ttctcctatt
    36121 gagcattttc ttggtagtag cctttgagat tatcaactct gctattgaaa atgtggtgga
    36181 tttggccagt cactatcact tttccatgct ggctaaaaat gccaaggata tggcggctgg
    36241 cgcggtatta gtggtttctc tttttgcagc cttaacaggc gcattgattt ttctcccacg
    36301 aatctgggat ttattatttt aaacagtaag aggaaattat gacttttaaa tcaggctttg
    36361 tagccatttt aggacgtccc aatgttggga agtcaacctt tttaaatcac gttatggggc
    36421 aaaagattgc catcatgagt gacaaggcgc agacaacgcg caataaaatc atgggaattt
    36481 acacgactga taaggagcaa attgtcttta tcgacacacc agggattcac aaacctaaaa
    36541 cagctctcgg agatttcatg gttgagtctg cctacagtac ccttcgcgaa gtggacactg
    36601 ttcttttcat ggtgcctgct gatgaagcgc gtggtaaggg ggacgatatg attattgagc
    36661 gtctcaaggc tgccaaggtt cctgtgattt tggtggtgaa taaaatcgat aaggttcatc
    36721 cagaccagct cttgtctcag attgatgact tccgtaatca aatggacttt aaggaaattg
    36781 ttccaatctc agcccttcag ggaaataacg tgtctcgtct agtggatatt ttgagtgaaa
    36841 atctggatga aggtttccaa tatttcccgt ctgatcaaat cacagaccat ccagaacgtt
    36901 tcttggtttc agaaatggtt cgcgagaaag tcttgcatct aactcgtgaa gagattccgc
    36961 attctgtagc agtagttgtt gactctatga aacgagacga agagacagac aaggttcaca
    37021 tccgtgcaac catcatggtc gagcgcgata gccaaaaagg gattatcatc ggtaaaggtg
    37081 gcgctatgct taagaaaatc ggtagcatgg cccgtcgtga tatcgaactc atgctaggag
    37141 acaaggtctt cctagaaacc tgggtcaagg tcaagaaaaa ctggcgcgat aaaaagctag
    37201 atttggctga ctttggctat aatgaaagag aatactaagt agaggtaggc tcatgcctgc
    37261 ttcttgtttt tacagaagga ggacttatgc ctgaattacc tgaggttgaa accgtttgtc
    37321 gtggcttaga aaaattgatt ataggaaaga agatttcgag tatagaaatt cgctacccca
    37381 agatgattaa gacggatttg gaagagtttc aaagggaatt gcctagtcag attatcgagt
    37441 caatgggacg tcgtggaaaa tatttgcttt tttatctgac agacaaggtc ttgatttccc
    37501 atttgcggat ggagggcaag tatttttact atccagacca aggacctgaa cgcaagcatg
    37561 cccatgtttt ctttcatttt gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt
    37621 ttggaaccat ggaactcttg gtgcctgacc ttttagacgc ctactttatt tctaaaaaat
    37681 taggtcctga accaagcgaa caagactttg atttacaggt ctttcaatct gcccttgcca
    37741 agtccaaaaa gcctatcaaa tcccatctcc tagaccagac cttggtagct ggacttggca
    37801 atatctatgt ggatgaggtt ctctggcgag ctcaggttca tccagctaga tcttcccaga
    37861 ctttgacagc agaagaagcg acagctattc atgaccaaac cattgctgtt ttgagccagg
    37921 ctgttgaaaa aggcggttcc accattcgga cctataccaa tgcctttggg gaagatggaa
    37981 gcatgcagga ttttcatcag gtctatgata agactggtca aggatgtgta cgctgtggta
    38041 ccatcattga gaaaattcaa ctaggtggac gtggaaccca cttttgtcca aactgtcaaa
    38101 ggagggactg atgggaaaaa tcatcggaat cactggggga attgcctctg gtaagtcaac
    38161 tgtgacaaat tttctaagac agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca
    38221 ccaactacag aaacctggtg gtcgtctgtt tgaggctcta gtacagcact ttgggcaaga
    38281 aatcattctt gaaaacggag aactcaatcg ccctctccta gctagtctca tcttttcaaa
    38341 tcctgatgaa cgagaatggt ctaagcaaat tcaaggggag attatccgtg aggaactggc
    38401 tactttgaga gaacagttgg ctcagacaga agagattttc ttcatggata ttcccctact
    38461 ttttgagcag gactacagcg attggtttgc tgagacttgg ttggtctatg tggaccgaga
    38521 tgcccaagtg gaacgcttaa tgaaaaggga ccagttgtcc aaagatgaag ctgagtctcg
    38581 tctggcagcc cagtggcctt tagaaaaaaa gaaagatttg gccagccagg ttcttgataa
    38641 taatggcaat cagaaccagc ttcttaatca agtgcatatc cttcttgagg gaggtaggca
    38701 agatgacaga gattaactgg aaggataatc tgcgcattgc ctggtttgat aattttctga
    38761 caggagccag tatttctttg gttgtacctt ttatgcccat cttcgtggaa aatctaggtg
    38821 tagggagtca gcaagtcgct ttttatgcag gcttagcaat ttctgtctct gctatttccg
    38881 cggcgctctt ttctcctatt tggggtattc ttgctgacaa atacggccga aaacccatga
    38941 tgattcgggc aggtcttgct atgactatca ctatgggagg cttggccttt gtcccaaata
    39001 tctattggtt aatctttctt cgtttactaa acggtgtatt tgcaggtttt gttcctaatg
    39061 caacggcact gatagccagt caggttccaa aggagaaatc aggctctgcc ttaggtactt
    39121 tgtctacagg cgtagttgca ggtactctaa ctggtccctt tattggtggc tttatcgcag
    39181 aattatttgg cattcgtaca gttttcttac tggttggtag ttttctattt ttagctgcta
    39241 ttttgactat ttgctttatc aaggaagatt ttcaaccagt agccaaggaa aaggctattc
    39301 caacaaagga attgtttact tcagttaaat atccctatct tttgctcaat ctctttttaa
    39361 ccagttttgt catccaattt tcagctcaat cgattggccc tattttggct ctttatgtac
    39421 gcgacttagg gcagacagag aatcttcttt ttgtctctgg tttgattgtg tccagtatgg
    39481 gcttttccag catgatgagt gcaggagtca tgggcaagct aggtgacaag gtgggcaatc
    39541 atcgtctctt ggttgtcgcc cagttttatt cagtcatcat ctatctcctc tgtgccaatg
    39601 cctctagccc ccttcaacta ggactctatc gtttcctctt tggattggga accggtgcct
    39661 tgattcccgg ggttaatgcc ctactcagca aaatgactcc caaagccggc atttcgaggg
    39721 tctttgcctt caatcaggta ttcttttatc tgggaggtgt tgttggtccc atggcaggtt
    39781 ctgcagtagc aggtcaattt ggctaccatg ctgtctttta tgcgacaagc ctttgtgttg
    39841 cctttagttg tctctttaac ctgattcaat ttcgaacatt attaaaagta aaggaaatct
    39901 agtgcgagta aaaattaatc tcaaatgctc ctcttgtggc agtatcaatt acctaaccag
    39961 taaaaattca aaaacccatc cagacaagat tgaggtttta aagtattgtc ccaaggaaag
    40021 aaaagtaacc ctacatcttg aatctaagta gattttatgg taaaataata gggattttaa
    40081 ggagtttgat atgtataacc tattattaac cattttatta gtattatctg ttgtgattgt
    40141 gattgcaatt ttcatgcaac caaccaaaaa ccaatccagc aatgtatttg atgccagttc
    40201 aggtgatttg tttgaacgca gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac
    40261 agggatttta gtctttttct ggctagccat tgccttagca ttgacggtat tatcaagtag
    40321 ataagaaaat aatgggcagg actaggtctt tgcctctttt tatttttaaa ggatgtttga
    40381 gaaggtttta cagtaaaaga aaattaaaaa atctagaaag aaaatatgaa agatagaata
    40441 aaagaatatt tacaagacaa gggaaaggtg actgttaatg atttggctca ggctttggga
    40501 aaagacagtt ccaaggattt tcgtgagttg attaaaacct tgtccttaat ggaaagaaag
    40561 caccagattc gctttgaaga agatggtagt ctgacattag aaattaagaa aaaacatgag
    40621 attaccctca aggggatttt tcatgcccat aaaaatggct ttggctttgt tagtctggaa
    40681 ggcgaggagg acgacctttt tgtagggaaa aatgatgtca actatgctat tgatggtgat
    40741 accgtcgagg tagtgattaa gaaagtcgct gaccgcaata agggaacagc agcagaagcc
    40801 aaaattattg atatcctaga acacagtttg acaacagttg tcgggcaaat cgttctggat
    40861 caggaaaagc ctaagtatgc tggctatatt cgttcaaaaa atcagaaaat cagtcaaccg
    40921 atttatgtta agaaaccagc cctaaaatta gaaggaacag aagttctcaa agtctttatc
    40981 gataaatacc caagcaagaa acatgatttc tttgtcgcga gtgttctcga tgtagtgggg
    41041 cactcaacgg atgtcggaat tgatgttctt gaggtcttgg aatcaatgga cattgtatct
    41101 gagtttccag aagctgttgt caaggaagca gaaagtgtgc ctgatgctcc gtctcaaaag
    41161 gatatggaag gtcgtttgga tctaagagat gaaattacct ttaccattga cggtgcggat
    41221 gccaaggact tggacgatgc agtgcatatc aaggctctga aaaatggcaa tctggagctt
    41281 ggggttcaca tcgcagatgt ttcctattat gtgaccgagg gttctgccct tgacaaggaa
    41341 gcccttaacc gtgcgacttc tgtttacgtg acagaccgag tggtgccaat gcttccagaa
    41401 cgactatcaa atggcatctg ctctctcaat ccccaagttg accgcctgac ccagtctgct
    41461 attatggaga ttgataaaca tggtcgtgtg gtcaactata ccattacaca aacagttatc
    41521 aagaccagtt ttcgtatgac ctatagcgat gtcaatgata tcctagctgg cgatgaagaa
    41581 aagagaaaag aataccataa aattgtacca agtatcgaac tcatggccaa gcttcatgaa
    41641 actttagaaa acatgcgtgt gaaacgtgga gctctcaatt ttgataccaa tgaagcgaag
    41701 attttagtgg ataaacaagg taagcctgtt gatatcgttc ttcggcagcg tggcattgcc
    41761 gagcggatga ttgagtcttt tatgttgatg gctaatgaaa cagttgccga acatttcagc
    41821 aagttggatt tgccttttat ctaccgaatt cacgaggagc ctaaggctga aaaggttcag
    41881 aagtttattg attatgcttc gagttttggc ttgcgcattt atgggactgc cagtgagatt
    41941 agccaggagg cacttcaaga catcatgcgt gctgttgagg gagaacctta tgcagatgta
    42001 ttgtccatga tgcttcttcg ctctatgcag caggctcgtt attcggagca caatcacggc
    42061 cactatggac tagctgctga ctattatact cactttacca gtccaattcg tcgttatcca
    42121 gaccttcttg ttcaccgtat gattcgggat tacggctgtt ctaaggaaat agcagagcat
    42181 tttgaacaag tgattccaga gattgcgacc cagtcttcca accgtgaacg tcgtgccata
    42241 gaagctgagc gtgaagtcga agccatgaaa aaggctgagt atatggaaga atacgtgggt
    42301 gaagagtatg atgcagttgt atcaagtatt gtcaaattcg gtctctttgt cgaattgcca
    42361 aacacagttg aaggcttgat tcacatcact aatctacctg aattttatca tttcaatgag
    42421 cgtgatttga ctcttcgtgg agaaaaatca ggtatcactt tccgagtggg tcagcagatc
    42481 cgtatccgtg ttgaaagagc ggataaaatg actggagaga ttgatttttc attcgtacct
    42541 agtgagtttg atgtgattga aaaaggcttg aaacagtcta gtcatagtgg cagagggcgt
    42601 ggttcaaatc gtcgttcgga taagaaggaa gacaagagaa aatcaggacg ctcaaatgat
    42661 aagcgtaagt attcacaaaa agacaagaag aaaaaaggaa agaaaccttt ttacaaggaa
    42721 gtagctaaga aaggagccaa gcatggcaaa gggcgaggga aaggtcgtcg cacacaataa
    42781 aaaggcacgc cacgactata caatcgtaga tacgctagag gcagggatgg tcctgactgg
    42841 aactgaaatc aagagtgtac gagctgctcg aattaatctc aaggatggct ttgctcaagt
    42901 gaaaaatgga gaagtttggc tgagcaatgt tcatatcgcg ccttacgaag agggcaatat
    42961 ctggaaccag gaaccagaac gtcgtcgtaa actcctgctc cataaaaagc aaattcaaaa
    43021 attggaacaa gagaccaaag ggacaggaat gaccttagtt ccccttaagg tctatataaa
    43081 agatggctac gctaagcttc ttttaggact tgccaaaggg aagcatgact atgacaaacg
    43141 ggagtctatc aaacgtcgtg agcaaaatcg agatatcgcg cgtgtgatga aagctgttaa
    43201 tcagcgataa aaagaggaat tgaaaatgga aaaattagtt gcctataaac gcatgccttt
    43261 gtggaataaa caaacaatgc ctgaagctgt tcagcaaaag cacaatacaa aagttgggac
    43321 ttgggggaaa attactgtct tgaagggagc tctcaagttt attgaattga cagaagaagg
    43381 ggaagttcta gctgaacacc tctttgaagc aggggcagac aatccaatgg cccaacctca
    43441 agcctggcac cgagtggaag ctgccacaga tgatgtggaa tggtacttgg aattttattg
    43501 taaacctgag gattattttg ctaaaaaata caataccaat cctgttcatt cagaggtcct
    43561 agaggccatg cagacagtga aacaagggaa agctttggat ttgggttgtg gtcaggggcg
    43621 taattctctt tttctagccc agcaagattt tgatgtgacg gctgtagatc aaaatggact
    43681 agctcttgaa atcttgcaaa gcattgtgga gcaggaagat ttggacatgc ctgttggcct
    43741 ttacgatatc aattcagcta gcattgaaca agaatatgat tttatcgttt caacagttgt
    43801 tctcatgttt ctacaagcgg accgcattcc agctattatt caaaatatgc aggagaaaac
    43861 cagtgttggt ggttacaacc ttatcgtttg tgccatggac acggaggatt atccttgctc
    43921 ggttaacttc ccattcacct ttaaagaagg agaactggca gactattaca aggattggga
    43981 attggttaag tacaatgaaa atccaggcca tttgcaccgt cgcgatgaga atggcaatcg
    44041 tattcaacta cgctttgcga ccttactagc taagaaaatc aagtaaacat acatgaagat
    44101 taggaatttt cctgatcttt tttctttttt acgaatgata tagaaaagga gggaattcat
    44161 gtttgttgcg agagatgcta ggggagaatt ggtaaatgtg ttagaggata aacttgagaa
    44221 gcaagcatac acctgcccag cttgtggagg ccagatccat ttgcgtcaag gaccaagtgt
    44281 acggacgcat tttgcccata aatccttaaa agactgtgat tttttctttg aaaatgaaag
    44341 tccagaacac ctggccaata aggaatccct ctatcactgg ttgaaaaaag agacaaaggt
    44401 tcaattagag tacccgcttt cagaacttaa acagattgcg gatgtatttg taaatggcaa
    44461 tctagctcta gaagttcagt gtagtccctt gcctcagaaa gtccttaaag agcgaagtga
    44521 gggctatcgt agtcagggtt accaagtact gtggttgctg ggtcaaaaac tgtggctcaa
    44581 ggagcgtttg actcgtctac agcaaggttt tctttatttc agtcaaaaca tgggctttta
    44641 tgtttgggaa ttagacaagg aaaaacaagt tttaagactc aaatacctga tttaccagga
    44701 tctccgcggt aaactccatt atcaaatcaa ggaattttcc tatggtcaag gtagtttatt
    44761 ggaaatattg cgtcttccct ataagagaca aaaaatatct cattttacag tttctgagga
    44821 caaggacatc tgtcgctata tccggcaaca actttattat caaaatctct tttggatgaa
    44881 agaacaagca gaagcctatc aaaagggaga aaatatcctg acttatggac tgaaagaatg
    44941 gtatccacaa attcgaccaa tagtgggcaa atttttccag attgaacaag acttgactag
    45001 ctattatcag cacttttata cctattacca aaaaaatcct caaaatgatt ggcaaaagct
    45061 ttatccacca gccttttatc agcaatattt cttgaaaaat atggtagaat agaaaggatg
    45121 gaggaatcta atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc
    45181 aacgatctac ccaactgacc aggcttggga agaagcctta aaagatttaa cagaacaatt
    45241 ggagacagta gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat
    45301 cactgaattt tctcttgaaa tggaacgcca gatagagaag ctttacgttt atgctcatat
    45361 gaagaatgac caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac
    45421 actctacagc cagttagacc aagccttttc attctatgag cctgaattta tggagattag
    45481 cgaaaagcag tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta
    45541 ttttgacaag ctcttgcaag gcaaggatca cgttctttca caacgtgaag aagaattatt
    45601 ggctggagct ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc
    45661 ggatattgtg ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg
    45721 gacttacaca cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc
    45781 tctttatgcg acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt
    45841 taaggtgcaa aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct
    45901 cgcagcgaat tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca
    45961 tttgccactc ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct
    46021 caagatgtac gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga
    46081 agccttgaaa aaagcagaag atgctttggc agtcttgggt gaggattact tgagccgtgt
    46141 taaacgtgcc ttcagcgagc gttggattga tgtttacgaa aatcaaggca agcgttcagg
    46201 tgcctactct ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa
    46261 tctggacaat ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac
    46321 tcgtgaaact cagccttatg tttacgggga ttactctatc tttttggctg agattgcctc
    46381 aactaccaat gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac
    46441 acgctttgct attctcaata acttcctaga tggtttccgt ggaacagttt tccgccaaac
    46501 tcaatttgct gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac
    46561 aagcgatttc ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa
    46621 ggaagacaat cctgaaatcc aatacgagtg ggctcgcatt ccacacttct actataacta
    46681 ctatgtatat caatattcaa ctggctttgc ggccgcctca gccttggctg aaaaaattgt
    46741 ccatggtagt caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga
    46801 ctatccactt aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa
    46861 cgatgccttt gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt
    46921 aggattggca taaaatggtt gaatcgtata gtaagaatgc taaccataac atgcgtcgtc
    46981 ctgtcgtcaa agaagaaatt gtagacttga tgcgtcagcg tcaaaagcag gtcacaggtt
    47041 tcttgaaaga attggaagac tttgcccgca aggaaaatat tcctattatt ccccatgaaa
    47101 cggttgctta tttccgtttt cttatggaaa ccatgcagcc taaaaatatt ctggaaattg
    47161 ggacggctat cggtttttca gctctcttga tggctgaaca tgcgccaaat gctaagatta
    47221 caactattga tcgtaatcca gaaatgattg gttttgccaa ggaaaatttt gcccagtttg
    47281 acagtcgcaa gcaaatcact ctcctagagg gagatgcggt ggatgtctta tctacactga
    47341 cagagtctta tgatttcgta tttatggatt ctgccaagtc taaatacatc gtctttctgc
    47401 cagaaatcct caaacatttg gaagttggtg gtgtggttgt cttggatgat atttttcaag
    47461 gtggtgatgt tgccaaggat attatggaag tccgtcgtgg tcagcgaacc atttatcgag
    47521 gccttcaaaa attatttgat gcaaccttag acaatccaga actcaccgca acattagtgc
    47581 ctttaggaga tggtattctc atgcttcgta aaaatgtagc agatgttcaa ctgtctgaaa
    47641 gcgaatgatt ttcagaaaaa tttaagaaaa aatagtaaaa tagatagagt aacacttatc
    47701 tcaaaggagt agacatgaag aaaaaattat tggcaggtgc catcacacta ttatcagtag
    47761 caactttagc agcttgttcg aaagggtcag aaggagcaga ccttatcagc atgaaagggg
    47821 atgtcatcac agaacatcaa ttttatgagc aagtgaaaaa caacccttca gcccaacaag
    47881 tcttgttaaa tatgaccatc caaaaagttt ttgaaaaaca atatggctca gagcttgatg
    47941 ataaagaggt tgatgatact attgccgaag aaaaaaaaca atatggcgaa aactaccaac
    48001 gtgtcttgtc acaagcaggt atgactcttg aaacacgtaa agctcaaatt cgtacaagta
    48061 aattagttga gttggcagtt aagaaggtag cagaagctga attgacagat gaagcctata
    48121 agaaagcctt tgatgagtac actccagatg taacggctca aatcatccgt cttaataatg
    48181 aagataaggc caaagaagtt ctcgaaaaag ccaaggcaga aggtgctgat tttgctcaat
    48241 tagccaaaga taattcaact gatgaaaaaa caaaagaaaa tggtggagaa attacctttg
    48301 attctgcttc aacagaagta cctgagcaag tcaaaaaagc cgctttcgct ttagatgtgg
    48361 atggtgtttc tgatgtgatt acagcaactg gcacacaagc ctacagtagc caatattaca
    48421 ttgtaaaact cactaagaaa acagaaaaat catctaatat tgatgactac aaagaaaaat
    48481 taaaaactgt tatcttgact caaaaacaaa atgattcaac atttgttcaa agcattatcg
    48541 gaaaagaatt gcaagcagcc aatatcaagg ttaaggacca agccttccaa aatatcttta
    48601 cccaatatat cggtggtgga gattcaagct caagcagtag tacatcaaac gaatagtcca
    48661 aatcaatgag tcagggaaaa aactctactt caggaaaaaa tgaagcaaac attcccacaa
    48721 taaaacgcgt agtacaaggt ttgtactgac ccccaaaagt tagacaaata atttatccga
    48781 aggatttagt tctgtattgc acagggctaa gtccttttag ttttacctta attctcttgt
    48841 tgttgtaata atcaatatag tctataatgg cttgttccaa ttgattaagt gatttaaatg
    48901 ttttctcata gccataaaac atttcggatt ttaaaatacc aaagaaagat tccatcctac
    48961 cgttgtcttg gctgtttccc ttgcgtgaca tagatgcttg aattccctta ctctctagga
    49021 accgatgata agaatcgtgt tggtattgcc agtcttggtc actatggaga atcgtattct
    49081 cgtagtgctt ctctttgaat gcctgttcca acatta
//
LOCUS       DAVMAF010000007        44395 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMAF010000007 DAVMAF010000000
VERSION     DAVMAF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.007"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(346..738)
                     /gene="rpsI"
                     /locus_tag="VYO97_000380"
     CDS             complement(346..738)
                     /gene="rpsI"
                     /locus_tag="VYO97_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1753243.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(757..1203)
                     /gene="rplM"
                     /locus_tag="VYO97_000381"
     CDS             complement(757..1203)
                     /gene="rplM"
                     /locus_tag="VYO97_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1753244.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1559..1876
                     /locus_tag="VYO97_000382"
     CDS             1559..1876
                     /locus_tag="VYO97_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1753245.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1921..2733)
                     /gene="folK"
                     /locus_tag="VYO97_000383"
     CDS             complement(1921..2733)
                     /gene="folK"
                     /locus_tag="VYO97_000383"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1753246.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVEHTFETYPLVQEMKLELKKPWA
                     PVHLPLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNRGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2892..3446)
                     /gene="folE"
                     /locus_tag="VYO97_000384"
     CDS             complement(2892..3446)
                     /gene="folE"
                     /locus_tag="VYO97_000384"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1753247.1"
                     /translation="MDKQKIEAAVKMIIEAVGEDSNREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3439..4749)
                     /locus_tag="VYO97_000385"
     CDS             complement(3439..4749)
                     /locus_tag="VYO97_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1753248.1"
                     /translation="MKEFENNQWITNYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     QSLLEGESAANLQGTTEFEIITAIAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAVAEQKAGIIKQGIPSVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGADYQVSHQESVVTGEVFDYTSVVRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     DGRKLASNHLLAQALEETSWPGRLEIVSRDPLMILDGAHNPHAIRALLATLQERFADY
                     HKEILFTCIKTKALEDMLNLLEQIPDTEVTLTHFDDSRATDESVLKEAAQSRNLSYQG
                     WQDFLEQKLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENG"
     gene            complement(4751..5728)
                     /gene="folP"
                     /locus_tag="VYO97_000386"
     CDS             complement(4751..5728)
                     /gene="folP"
                     /locus_tag="VYO97_000386"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1753249.1"
                     /translation="MMKSLEGVTDMSSKANHVKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYYVEIEEEIQRVVPVIKAIRKESNVLISID
                     TWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKARAKVVIMFNPVMARPQHPSSL
                     IFPHFGFGQTFTEKELADFETLPIEDLMEAFFDRALSKADEAGIAPENILLDPGIGFG
                     LTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETEHGFRNRDTA
                     SAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5824..6531)
                     /locus_tag="VYO97_000387"
     CDS             complement(5824..6531)
                     /locus_tag="VYO97_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1753250.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIIPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6585..8003)
                     /locus_tag="VYO97_000388"
     CDS             complement(6585..8003)
                     /locus_tag="VYO97_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1753251.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALIVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            8193..9005
                     /locus_tag="VYO97_000389"
     CDS             8193..9005
                     /locus_tag="VYO97_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1753252.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKNIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9344..10363
                     /gene="adhP"
                     /locus_tag="VYO97_000390"
     CDS             9344..10363
                     /gene="adhP"
                     /locus_tag="VYO97_000390"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1753253.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            10596..11585
                     /gene="manX"
                     /locus_tag="VYO97_000391"
     CDS             10596..11585
                     /gene="manX"
                     /locus_tag="VYO97_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1753254.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            11613..12416
                     /locus_tag="VYO97_000392"
     CDS             11613..12416
                     /locus_tag="VYO97_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1753255.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            12440..13351
                     /locus_tag="VYO97_000393"
     CDS             12440..13351
                     /locus_tag="VYO97_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1753256.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13407..14741)
                     /gene="pepC"
                     /locus_tag="VYO97_000394"
     CDS             complement(13407..14741)
                     /gene="pepC"
                     /locus_tag="VYO97_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1753257.1"
                     /translation="MNAIQESFTNKLFANYEANVKYKAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQTKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(14958..15680)
                     /locus_tag="VYO97_000395"
     CDS             complement(14958..15680)
                     /locus_tag="VYO97_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1753258.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(15903..16133)
                     /locus_tag="VYO97_000396"
     CDS             complement(15903..16133)
                     /locus_tag="VYO97_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1753259.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(16143..17384)
                     /locus_tag="VYO97_000397"
     CDS             complement(16143..17384)
                     /locus_tag="VYO97_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1753260.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKANMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(17406..17558)
                     /locus_tag="VYO97_000398"
     CDS             complement(17406..17558)
                     /locus_tag="VYO97_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753261.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            complement(17579..17857)
                     /locus_tag="VYO97_000399"
     CDS             complement(17579..17857)
                     /locus_tag="VYO97_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1753262.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(17850..18113)
                     /locus_tag="VYO97_000400"
     CDS             complement(17850..18113)
                     /locus_tag="VYO97_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1753263.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(18215..22606)
                     /locus_tag="VYO97_000401"
     CDS             complement(18215..22606)
                     /locus_tag="VYO97_000401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1753264.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(22710..24791)
                     /gene="fusA"
                     /locus_tag="VYO97_000402"
     CDS             complement(22710..24791)
                     /gene="fusA"
                     /locus_tag="VYO97_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1753265.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(25216..25686)
                     /gene="rpsG"
                     /locus_tag="VYO97_000403"
     CDS             complement(25216..25686)
                     /gene="rpsG"
                     /locus_tag="VYO97_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1753266.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(25706..26119)
                     /gene="rpsL"
                     /locus_tag="VYO97_000404"
     CDS             complement(25706..26119)
                     /gene="rpsL"
                     /locus_tag="VYO97_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1753267.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            26536..30333
                     /gene="spuA"
                     /locus_tag="VYO97_000405"
     CDS             26536..30333
                     /gene="spuA"
                     /locus_tag="VYO97_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1753268.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSILIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNDVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(30433..31482)
                     /locus_tag="VYO97_000406"
     CDS             complement(30433..31482)
                     /locus_tag="VYO97_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1753269.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            31539..31616
                     /locus_tag="VYO97_000407"
                     /pseudo
     CDS             31539..31616
                     /locus_tag="VYO97_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(31753..33561)
                     /gene="glmS"
                     /locus_tag="VYO97_000408"
     CDS             complement(31753..33561)
                     /gene="glmS"
                     /locus_tag="VYO97_000408"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1753270.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSKTERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(33731..35110)
                     /gene="bglA"
                     /locus_tag="VYO97_000409"
     CDS             complement(33731..35110)
                     /gene="bglA"
                     /locus_tag="VYO97_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1753271.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(35210..37063)
                     /locus_tag="VYO97_000410"
     CDS             complement(35210..37063)
                     /locus_tag="VYO97_000410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1753272.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(37076..38335)
                     /gene="rseP"
                     /locus_tag="VYO97_000411"
     CDS             complement(37076..38335)
                     /gene="rseP"
                     /locus_tag="VYO97_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1753273.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(38357..39160)
                     /locus_tag="VYO97_000412"
     CDS             complement(38357..39160)
                     /locus_tag="VYO97_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1753274.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(39169..39927)
                     /locus_tag="VYO97_000413"
     CDS             complement(39169..39927)
                     /locus_tag="VYO97_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1753275.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDCLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(40158..40730)
                     /locus_tag="VYO97_000414"
     CDS             complement(40158..40730)
                     /locus_tag="VYO97_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1753276.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIGVIFFDPDISYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYIPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(40711..41682)
                     /gene="ruvB"
                     /locus_tag="VYO97_000415"
     CDS             complement(40711..41682)
                     /gene="ruvB"
                     /locus_tag="VYO97_000415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1753277.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            41842..41934
                     /locus_tag="VYO97_000416"
     CDS             41842..41934
                     /locus_tag="VYO97_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1753278.1"
                     /translation="MMELVLKTIIEPIVVGVVLRLVDKWLNKDK"
     gene            42275..42367
                     /locus_tag="VYO97_000417"
     CDS             42275..42367
                     /locus_tag="VYO97_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1753279.1"
                     /translation="MMELILKTIIGPIVVGVILRLVDKWLNKDR"
     gene            complement(43218..43634)
                     /locus_tag="VYO97_000418"
     CDS             complement(43218..43634)
                     /locus_tag="VYO97_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753280.1"
                     /translation="MITIKKQEFVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLAI
                     YVALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSTLMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYCSMGFEILSTYDCIGMTWINREK"
     gene            complement(43646..44341)
                     /locus_tag="VYO97_000419"
     CDS             complement(43646..44341)
                     /locus_tag="VYO97_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753281.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELTFIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDRSTGKVLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAF
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMTDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREL"
BASE COUNT        12956 a        10248 c         8523 g        12668 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga attcgatttg
       61 actatcctcg gtttgtccta ttcttatttc accttactat aaaaataagg taaacttttc
      121 aaaaaaacac gaaaaaagca cccatagggc aaacctttga gtgctttgaa acattgatat
      181 aattgtattg attagcgttt tgaggcaact gaccttgtca ggttgcagcc acatttgtaa
      241 gcgactaaag tcgcaacaac tgtgtcaatt gcaccaattt agtaagaaag tataaaaaag
      301 aacaccccga aaggtgctct gtataagtat agtaattctt tcgaattaac gtttactaaa
      361 ttgtgatgct ttacgagctt tcttaagacc tggtttctta cgttcaactt tacgtgagtc
      421 acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag tctgggtcta cttgaagaag
      481 ggcacgagcg ataccgtgac ggatagctcc tgattgacca gcgtatccac cacctacaac
      541 gttaacgaaa acgtcgtatg aacctacagt tgaagtaact gcaaatggtt ggttgatgac
      601 aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct tttttgttaa cagtgatttt
      661 accagttcct ggaacaaggc gaacgcgtgc aacagcgttt ttacgacgtc cagtacctgc
      721 atattgtgct tgtgacatac tttattgttc ctttccttag ataagtcctg aaatgtcaag
      781 aacttctggt tgttgtgcag cgtgagtgtg ctcggctcca acaaatactt tcaacttcat
      841 accttgagcg cgtccaagag tattgtgtgg aagcatacct ttaactgatt tctcgatcaa
      901 acgtactgca tttttagaac gaagttcacc tgcagagatt tgtttcaatc cacctgggtg
      961 gtttgagtga gtgtagtaga ttttatcagt tgctttttta ccagtcaatt taactttttc
     1021 agcattgata acaatcacga agtcacctgt atcagtgtgt ggtgtaaatg ttggtttgtt
     1081 ttttccgcga agtacgctag caactactgc agaaagacgt ccaagtggta catcagttgc
     1141 gtcaactacg taccatttac gttcaacttg gcctggttta gccataaatg ttgttttgtt
     1201 catgatttct cctatatata gttcgatttt tgtttacggt gatgggtgtt ccttcccatc
     1261 gaaaagtatt tggaaggttc cggggccttt caaatggggt aaacaatacc gcctactatc
     1321 ataccaaatt tcacccctaa aagtcaatag atataaaaaa tatttttcgg taacttctca
     1381 aaataactga aagttatata atcaagttaa tttgggaatt tacctattaa cttcggtaga
     1441 tttttgggaa ttttttccct atttctccct gtaactaaaa tccttttcaa aaaatcacct
     1501 tttcttgatt tttaatccta tttgctgtat gataagggaa aagaaagggg acagagatat
     1561 ggcttttacc aatacccaca tgcggtctgc tagttttggc attgttacca gcttgcccga
     1621 tgacatcatt gactcttttt ggtatatcat cgatcatttc ttaaaaaatg tctttgaatt
     1681 ggaagaagaa ctcgagtttc aattgcttaa taaccaagga aagattacct tccacttttc
     1741 aagtcaacac ctccctacag ccattgattt tgactttaac catcctttcg accctcgtta
     1801 tcccccaaga gtactggttt tagacatgga cggtagagaa actatcctcc tcccagaaga
     1861 aaatgaccta ttttaaaaac tctagccttc agttgcaagt aactgaaaac tagagttttt
     1921 ctattttttc aaagcatcat acaagttgcg gatcggttgt tttaatatcg gatggataaa
     1981 atgaggcgca atttcctgta aggactcaag gacaaaaagg cgttccgcta tgtaaggatg
     2041 aggcaatatg aggtcgtctg tataaaggat ctggtcctcc acaaagagca agtccaaatc
     2101 aatcaaacga ggtccccaat gcacttctct cacccgtccc agctctgact caatggctaa
     2161 caaggtttct aacaagtctt gtgctggtag ccaggtttcc acctcaacca cttgattggc
     2221 aaagctatcc tgctccactc caccccaagg ctccgtcgct aagacactgg actctttgag
     2281 aatatggata cctcgatttc gcattttctc aatggcttgt ttcaagtttg cttgtttatc
     2341 tcccatattg cttcctaggg cgataaaggc tcgttgctta cggcgatgga tggttacgga
     2401 gcaagtatct aacggcaaat gcactggcgc ccaaggtttt ttcagttcca gcttcatttc
     2461 ttggacaaga ggataggtct caaaggtatg ttctaccagt ttgtaggcta ccgtttcaat
     2521 caaatcttca ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg
     2581 gacagaggct gttaaatcca agtctgtagc tgccttggtc atatcatagg ataggatggc
     2641 tgaaacgaca aatttctgcc ccaattcttt ctcactagga aaaagaccat gataggcaaa
     2701 catttccaaa tccttaatct gcagttgatc cataatgatt cctttctcga aaaatccgcc
     2761 caaagcggat tctttttata ctcaatgaaa atcaaagtgc aaactaagaa gctagccgca
     2821 ggctactcaa aacactgttt tgaggttgca gatggaagct gacgtggttt gaatttgatt
     2881 ttcgaagagt attatagtcc cattaaacga taagcctgat ctcggaggtc cttatctgtt
     2941 tcaaatagac cacgagctac tgtcgtcaag gttgcagtgc ctggttttct gacaccacgc
     3001 atactcatac acatatgttc cgcctcaatg acaacaaagg ctcctttagc gcctagatag
     3061 tccatcaagg catcggccac ttcgatattc aaacgttctt gaatttgtgg ttttttcgaa
     3121 taaacttcaa ccgtacgggc tagcttagat aaacctgcta cacggccatc tggaatatag
     3181 gcaatatgcg ctctaccata aaatggcaag aagtggtgtt cacacatggt atggaaaaag
     3241 atatcctttt ctaccaccat attatcgtca ataatctcaa aagattttga caaatgttcc
     3301 tccgctgttt gaccaagacc tgaaaaaatc tcttggtaca tacgagccac acgagcaggt
     3361 gtttcctgca agccctcgcg gttagagtcc tctcccacag cctcgataat catttttaca
     3421 gctgcttcaa tcttttgttt atccattctc gttcttcctc tccatcagat aggctctcac
     3481 ttggctcaag aaatacaagg aacctgtgac aatcctaact gtttgtttct cttctttttt
     3541 atctgtcaat ttctgctcta gaaaatcctg ccaaccttgg tagctgagat ttctagactg
     3601 agctgcctct ttcagcacgc tttcatcagt cgcccgacta tcgtcaaaat gtgtcagggt
     3661 aacctcggta tctgggattt gctccaacaa attcaacata tcctccaagg ccttggtttt
     3721 gatgcaagta aagaggattt ccttatgata atccgcaaag cgttcttgca aggttgccaa
     3781 taaagccctg atagcatggg gattgtgggc cccatccaaa atcatcaagg gatctcttga
     3841 cacgatttcc aaacgccctg gccaacttgt ttcttccaag gcctgagcaa gcaagtgatt
     3901 gcttgctagt tttcgcccat cttcttgaca aaaagtatca agtaaagcta tggccatccc
     3961 agcattctct atctggtgca aaccaagcag gccagtctgg aagcgacctt gtctgacaac
     4021 acttgtatag tcaaagactt cacctgttac cacactctct tggtgactaa cttgataatc
     4081 tgccccgtag gcaagtctcg gcgcatcttt cccttccgca atgcggtcaa taacagccaa
     4141 ggcttctgga gcaatgcgac ctgtcaccga gggaatgcct tgcttaataa taccagcctt
     4201 ctgctctgct acggcttcca aggtgtcacc aagtagggct acatgatcca gtccaatggt
     4261 cgtgatgcct gttagaatgg gctggcagac attggtacta tccaagagcc cacccatgcc
     4321 aacttccata atagccacat ctacttgctc tgaggcaaag taatcatagg ctatagctgt
     4381 aattatctca aactcagttg tcccctgcaa attagcagcc gattccccct caagcagcga
     4441 ctgatagtct gccatgagag cttctagtct agcttctggg atagattccc cattgatgct
     4501 aatctggtct gtgtaatgaa tgagataggg cgagctaaac acgccaactc tcagccctag
     4561 tttttctagc atatttttca aaaaagcaat ggtcgaaccc ttgccattag ttcctccaat
     4621 atggatgacc ttgagtttga gatggggatt gccacgcaaa gctaacagtt ccaccattcg
     4681 ttccaagcca aaatgcggtt ggtccgtccg gtaattggta atccactgat tgttttcaaa
     4741 ttctttcatc ttatttgtat tgttttaaat ctaaattttc cgcttcatca gccagacgaa
     4801 tagcggaggc aatttcaact gccatcctgt gactagctac gtcatgcacg cgcaccactt
     4861 ctacaccctg tctcgcagcg atactagtta catgagccga agctgtgtcc cgattgcgga
     4921 aaccatgttc tgtctcagga ttgacttcaa acccactttc ttcaaggata ttgatgacaa
     4981 atcgcttgcg cgaaactccg agaaagattg gatagccttt ctgatggagt ttatccagat
     5041 cccgtaaaag aagcagattt tctttcttag tcagaccaaa gccaattcct ggatccagca
     5101 ggatattttc tggtgcaata ccagcttcgt ccgctttcga tagggctctg tcaaagaaag
     5161 cctccatcaa gtcttcgatt ggcaatgttt caaagtcagc taactctttt tctgtaaagg
     5221 tttgaccaaa gccaaaatga gggaagataa gcgaactagg atgctgaggt cgagccataa
     5281 ctggattaaa catgatgacc actttcgctc tcgctttagc taccacatga gccatttttt
     5341 catcacccat gagaccagtg atatcattaa ctagattagc accagcagcc aaagcagctt
     5401 ctgctacctg actcttccaa gtatcaatag agatgaggac attactttcc ttgcgaatcg
     5461 ctttgatcac tggaacaaca cgctggattt cctcttctat ctcaacataa tagctactgc
     5521 ccggccgagt cgattctccg ccgatatcta gcatactggc tccttctgct atcaatttac
     5581 gagcctgctg gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa aaggagtctg
     5641 gggttacatt gataattccg caaatagctg tctttacatg attggcttta cttgacatat
     5701 cggtcactcc ctcaaggctt ttcatcatat tatttctcta ttttaccata aaaagaaaaa
     5761 gatggccacg attgcattca tctttttccc agtagaaaga ggtcggttcc tacataaaaa
     5821 cttttaaaca gaaattccta atgtccgact cataatcacc acaagagcca acaaacagaa
     5881 agcaatccca ttaacaatca tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta
     5941 ggctgtccaa gatagaactg tcgacatacc tccataaatc aataaagaag gtaaattact
     6001 tggttgatgc aataaagcaa acacaatcgt accgactaca aatcccaagt tctccttgcc
     6061 tcggaaaatc tttttaggaa taatcccacg acacaagatt tcctcacaaa tcggagcaag
     6121 caaggctagc aagaagaaac tggaaatcaa cgaactattt tgaaccatat cattaatctg
     6181 agactggtta gctgttgtcg tctcatttga cagttgcaat aaaatggaac caagtatatt
     6241 tgacccgaca ataactagat aactcaagcc caaacgtgcc aaatctttag ctctaaaaaa
     6301 agaaaaatta aaactagcta acttggtttt acgagctccc ataataaata gagccagaac
     6361 cacaattgaa aggccagcaa ctatcagccc tgactgtagc agggctactt cttttaaagt
     6421 caaaatagag gtaaccacta ggggaacctg atataaaagg acggcaagta aaaagaccaa
     6481 aatccagcct gcacgattca acaattcttt ccacatattt ttctctttca tcacctatct
     6541 ccttttgtat taaaaaaggg gggaaatccc cctgggtcat tctattataa ggccatgctg
     6601 atgtagttaa ggataaacaa ggcatccaaa atccaaatca tgacatgaac atctttaact
     6661 tgacctttaa caagcttagt caaagtgtaa gtcaagaaac caactgcaat cccttgagtg
     6721 atagagtagc tgaatcccat aaagatagat gtgaagaagg caggaactgc ttcagacata
     6781 tcatcccaat ggatattttt caagctacca agcatcataa tcccaacgat aatcaagatt
     6841 ggagctgtag ccgctgttgg tacgatcgct agaagtgggc taaagaagct tgaaatcgca
     6901 aaacagatag ctacaatcaa ggctgtcaaa ccagtacgtc cacctgcacc gataccagca
     6961 gcagactcaa cataagtcgt tacgtttgaa gtacctgcaa tggcaccgac tgtcgttcca
     7021 atcaaatcag agtaaagagc cttatccaat ttggctgatt ggtgattttc accatttgtc
     7081 gctacgatac caactttttc acctgtaccg atcaaggtac caattgtgtc aaaaatatct
     7141 gtcaatgaga aggcaagaat ggccatcaga gtttcaggca agcgagctgt atctgaaacc
     7201 aaagctccta aaccttctga accaagagct gcaccaaaga ttgtcttcaa atcttcaaaa
     7261 gctgcaccaa catggttatt agcaaaatcg atactagaca aatcaaccaa accaactgca
     7321 atagcaagaa cagttgttgt caagatagag agaataattc cccctttaat ccctttgatg
     7381 acaaagaaga tagtaatggc aagtcctgca agagccacta aaacagctgg attattaaag
     7441 ctgaccaatc ctggaactgc tgaagagttt gctgcaatcg ttgcttgagc tttgtcagcc
     7501 ccttctccta caacagtata gttgcctgga tcaatcgtga atttcaaaag tccagcattc
     7561 ttaatcccta cataggcaag gaagacaccg ataccagctg aaatagctga gcgaagagca
     7621 ttgggaatcg attcaatgat cattttacga acatttgtca aggtaataat caatgaaata
     7681 atcccacaga tgaagaccat agctagggct tcttgccaag aataaccaag cccgaataca
     7741 actgtaaagg taaagaaggc attgagtccc atacctggcg cttgggcata aggtaagtta
     7801 gcataaaaag ccatcatcaa ggtacccgct actgcaccaa taatcgtcgc taggaagacg
     7861 ccctgagcag gcattcctgt ttgtgaaagt atttgtgggt ttacaaagag aatatagctc
     7921 attgcaaaga aagttgttaa accagcgaga acctctgtac gaacgtctgt accgttctct
     7981 tttagtttaa ataatttgtc catcatcaat ctccttttaa tttttacgaa caataataga
     8041 attatatcat ttatcattca ctttttcaat gtctttgttt gatttgttta agaaattaat
     8101 ccaatttcat tttttgtttg gaatctgctt ttctgcctgc tttctgttat aatagaagac
     8161 atcttatctg aaaacacact agaaaggtcc atatgacgaa aaaaattatt gcagttgacc
     8221 tggatggaac cctgctcaac tcagacagtc aaatttctga ctttaccaaa aaaaacatca
     8281 aaaaagttgc tgaaaaaggc catcaggtta ttattacgac aggtcgccct taccgtatgt
     8341 caaaagattt ttaccgtgaa ctgggcttag acactcctat gattaacttc aacggatccc
     8401 ttactcattt accagaccaa gtttgggatt ttgaaaagtg tttgactgta gacaaaaaat
     8461 atctgctaga tatggttcaa cgttcagagg acattcaagc cgattttatc gctggagaat
     8521 atcgtaaaaa attctacatt acaaatccca atgaagaaat tgccaatccc aaactatttg
     8581 gtgtagaagc tttccagcct gaagatcaat tccagcctga attggtgacc aaggacccta
     8641 actgtatcct cttgcagacc agagccagtg acaaatatgc cttggcaaaa gacatgaacg
     8701 ccttctacca gcatcaactt tctatcaata cctggggagg tccgctcaat atccttgaat
     8761 gtaccccaaa aggtgtcaac aaggcctttg ctttggacta cttgctcaag ataatgaatc
     8821 gtgacaaaaa agatttgatt gcctttggag atgaacacaa tgataccgaa atgctcgctt
     8881 ttgctgggaa gggttatgcc atgaaaaatg ccaatccaga gctactccct tatgcagatg
     8941 agcaaatttc ccttaccaac gaccaagatg gggttgccaa aaccctacaa gacttattct
     9001 tataacctat actgatactc aatgagaatc aaagagcaaa ctaggaagct aaccgtaggt
     9061 tgctcaaagc actgctttga ggttgtagat gaagctgacg tggtttgaag agattttcga
     9121 agagtatgac cagcttgcaa gctggtcttt gtgctctttt cacagtctca tcaaccagtt
     9181 aatcgattgt tctacttttt gtctccaaaa ccttggaaaa ggctttcttt tgtgatatac
     9241 ttcacatata aatacaagag agctcgtcac actcgactct gttgacaaaa aatcaatcca
     9301 gtcccgttgt ccctgttctc ggtcaatatc aaggaggata gctatgaaag ctgttgttgt
     9361 aaatccagaa agcactggtg ttgctattga agaaaaagta ctccgtccac ttgaaactgg
     9421 ggaagcactt gtagaagttg aatactgtgg cgtttgccac accgacctcc acgttgctca
     9481 tggtgacttt ggtcaagtcc caggacgtgt tcttgggcac gaaggtatcg gtatcgttaa
     9541 agagattgct ccagatgtga aaagccttaa agtcggtgac cgcgtcagcg ttgcttggtt
     9601 ctttgaagga tgtggcactt gcgaatactg tacaactggt cgcgaaaccc tttgccgtac
     9661 agtgaaaaat gctggctact cagtagacgg tggtatggct gaacaatgta tcgtaactgc
     9721 tgactatgct gtcaaagttc ctgacggact tgatccagcc caagcttctt ctatcacatg
     9781 tgctggagta acaacctata aagctatcaa agaagcaaaa gttgaaccag gccaatgggt
     9841 tgttctttac ggtgctggtg gacttggtaa cctcgctgtt caatacgcta aaaaagtatt
     9901 caatgctcat gttatcgcag tcgatatcaa caatgacaaa cttgcccttg caaaagaagt
     9961 aggcgctgac attgtgatta acggcctcga agttgaagat gtagctggac tcattaaaga
    10021 aaaaactgat ggaggagctc attcagctgt cgtaactgct gtgtctaaag ttgccttcaa
    10081 ccaggctgtt gactccattc gtgctggtgg tcgcgtcgtc gctgttggtc ttccttctga
    10141 aatgatggaa ctcagcatcg ttaaaacagt cctcgatgga atccaagtca tcggttctct
    10201 tgtcggaact cgtaaagact tagaagaagc cttccaattt ggtgcagaag gtctagtagt
    10261 cccagttgtt caaaaacgtc cagtagaaga tgctgttgcc attttcgacg aaatggaaaa
    10321 aggccaaatc caaggacgta tggtactcga cttcacccac taatctaatg gaaacctatt
    10381 ttaaaagttg gaagcttctt ccaacttttt tatattaaat tttaaataag aattcaagat
    10441 tactcctcaa aaactcaaca tatcaacata caaaaataaa aattttttaa ttttttatga
    10501 taaatagttg ctttttttat gaaaacggtt tatacttaaa cagtcttaaa gttttcttaa
    10561 atgaaaacta aaacaaaaaa ggaggaatga caataatgag tatcggaatc attattgcga
    10621 gccacggcga atttgctgcg ggtattcatc agtcaggatc tatgatcttt ggtgaacaag
    10681 aaaaggttca agttgtgacc tttatgccaa atgaaggtcc tgatgatcta tacgctaagt
    10741 ttaataacgc tgttgctgca tttgacgcag aagatgaggt tctagttttg gctgaccttt
    10801 ggagtggttc tccatttaac caagctagtc gcgtgatggg agaaaatcct gagcgtaagt
    10861 ttgccatcat cacaggactt aacttaccga tgttgattca agcctacaca gagcgcctca
    10921 tggacgctgc tgcaggtgta gaaaaagtcg ctgctaatat cattaaagaa gccaaagatg
    10981 gcatcaaagc tcttccagaa gagctaaatc cagtcgaaga agttgcaagc gctgcagctg
    11041 ctccagttgc ccaaactgct atcccagaag gaactgttat cggagacggt aaattgaaaa
    11101 tcaatcttgc ccgtcttgac acacgtctac ttcacggtca ggttgcaact gcttggactc
    11161 cagattcaaa agcaaatcgt atcatcgttg cttcagataa cgtggctaaa gacgaccttc
    11221 gtaaagaatt gattaaacaa gcagctccag gtaatgtcaa ggctaacgtg gttccaattc
    11281 aaaaactgat tgagatttca aaagacccac gttttggaga aacacatgcc cttatcttgt
    11341 ttgaaacacc tcaagatgcc cttcgtgcca tcgaaggcgg cgtgccaatc aagactctta
    11401 atgttggttc tatggctcac tcaacaggta aaacattggt caataccgtt ttgtctatgg
    11461 acaaagaaga cgttgctaca tttgaaaaaa tgcgtgactt gggtgttgaa tttgatgtcc
    11521 gtaaagtacc aaatgattct aaaaaagatt tgtttgactt gattaacaaa gccaatgtca
    11581 aataagccat tatttatgaa aggattttaa acatgtctat tatttctatg gttttagtag
    11641 tcgttgtagc cttctttgca ggtcttgaag gcatcctcga ccagttccaa tttcaccaac
    11701 cacttgtagc ctgtaccctt attgggcttg taacaggtca cttggaagca gggattatcc
    11761 tcggtggatc gcttcaaatg attgcccttg gttggtcaaa tatcggtgct gctatcgctc
    11821 ctgatgctgc acttgcttct gtcgctgctg ccattatcat ggttcttggt ggtgacttta
    11881 ccaagactgg tatcggtgtt gcccaagcgg ttgctatccc tcttgccgta gctggacttt
    11941 tcttgacaat gattgttcgt acaatttcag ttggtttggt tcatactgca gatgctgccg
    12001 ctaaaaaagg tgacttcggc gctgtggagc gtgctcattt cattgcgcta cttttccaag
    12061 gacttcgtat cgcgcttcct gcagctcttc tccttatggt accaactgaa actgtacaaa
    12121 gtatccttag tgccatgcca gactggctca aagatggtat ggctatcggt ggtggtatgg
    12181 tcgttgccgt tggttacgcc atggttatca acatgatggc aactcgtgaa gtatggccat
    12241 tcttcgctct tggtttcgtt ctcgctgctg tgtcagatat tactctaatc ggattcggtg
    12301 ctatcggtgt tgctatcgct cttatctacc ttcacctttc taaaactggt ggaaatggtg
    12361 gcggaggagc cgcaacttct aacgacccaa tcggcgatat cctagaagac tactaagata
    12421 agaaaggact gaaaacatca tgactgaaaa acttcaatta actaaatcag atcgtaaaaa
    12481 agtttggtgg cgttcaacct tcttacaagg gtcttggaac tttgaacgga tgcaaaactt
    12541 gggctgggct tatacactca ttccagctat caaaaaactc tatactaaaa aagaagatca
    12601 aatcgctgct cttgagcgtc accttgagtt cttcaacact catccatacg tagctgctcc
    12661 agtcatgggg gttactcttg cgcttgaaga agaacgtgct aacggtgtgg aaatcgatga
    12721 cgctgctatc caaggggtta aaatcggtat gatgggacct cttgctggta tcggtgaccc
    12781 agtattctgg tttacagtac gcccaatcct tggatctctc ggtgcttcac ttgcccttac
    12841 tggcaatatc ttggggccac tcctcttctt tgttgcatgg aacttgattc gtatgtcatt
    12901 cttgtggtat gttcaagaga ttggatacaa ggctggatca gaaatcacta aagatatgtc
    12961 tggtggtatc cttcaagata tcactaaagg agcttctatc cttgggatgt tcattcttgc
    13021 tgtccttgtt caacgttggg taaatattaa atttgctttc gatgtttcta aagttcaact
    13081 agatgaaaag gcttatatcc attgggataa attgccagaa gggtctaaag gtatccaaga
    13141 agcattcgca caagtaggac aaggattgtc tcaaactcct gaaaaagtta ctactttcca
    13201 acaaaacttg gatatgttga ttcctggatt atcaggacta ctccttactt tactttgcat
    13261 gtacttactt aagaaaaaag tatctccaat cactattatc cttgccctct tcgcagtggg
    13321 tattgtggca catgttcttc acatcatgta atcaagcaac taaaaaggaa ccaggttcta
    13381 aaatctgatt ccttttttct atgcttttat tcagccaagg ctcccattgg atcccatggt
    13441 gcaagtacga ttggttctgc tccataggca gcttgttctt ctgctgtcag caattcctta
    13501 cgaacaacga tttggtatgt gtattcgtcc atccaagcgt ctgaggcaac aaagtaacca
    13561 tctgtaccga ccttgtctcc ccatgagttt tcaaccttcc acttggttga tttaccattt
    13621 tcgtccaagt caacacctgt caagaccatg gcgtgggtca tcaagctttc gctatagtcc
    13681 aaacgtccag ccttgtcttg agtaagttta atgtccatgc ttgattcaaa gtcataaaca
    13741 tctgtcgcaa ggatgccagc cttacggttg ctgagctggc cgacatcaga accaaaccaa
    13801 acagtctcac ctgcttgcat ttgggcaatc gccaattctt tcaagcgctc cattggaacg
    13861 ttgatgtagc gaactgcacg gctaccaaca acattcccca acatctcaac tgtgtaagat
    13921 tttccgtaag gtttatcagc agttggagca ttgataacag aaacgtagtc ttctaaagga
    13981 agattgacat atttcttgta aaactcttgt ggtgtgattc ctttttcact tttgtagttg
    14041 ttatctttat cgcgataagc aaagtcaaac ttgcgtggtg gaagtcctaa tgacatagca
    14101 agaaagttaa agatttcttg caagaggtct tctttcttag tttgaacagt cgcttgatct
    14161 gcaccagaaa caagcaaatc acgcaagatt tgagcatctt gacgaagcaa tttattaagg
    14221 atcgcattta gctcacgact gctgctagat gaaacagact caggataaac tgacttaggc
    14281 acgacaccgt atttttcaaa gagggaaacg accatatccc attgaccgcc atcttgttga
    14341 ggtgtttgga gtaagaagct aaccttgcgg ctagtcaatt cttggtctga agtcgcaatg
    14401 acttgctcca agaaccagtt tgatttctca tacttatccc agaagaaagt gtgggcttgt
    14461 gacaactcaa agttctccaa tttgtattgc gagatgagtt tgtggcggaa ggtgttgaga
    14521 gccgcaaaca tccagcaacg accagacgct ttctggttag tgaccttgtc cttggttaaa
    14581 tccaatgaga aaacaggtgt gttgtctaca tggctttggc gacattccag agctgcaaaa
    14641 attccgttgt ggctggcagc attttcaatc gctttgtatt ttacatttgc ttcatagttg
    14701 gcaaatagtt tattagtaaa tgattcttga atcgcgttca tagattcctc cttttattct
    14761 acagtctatt ataactcttt tcacaaagga agcaactaaa aaacataaaa ggtaaggaca
    14821 ttctccctca cctttttcga atttatactc ttcgaaaatc tcttcaaacc acgtcaacgt
    14881 cgccttgccg tactcaagta cagcctgcgg ctagtttcct agtttgctct ttgattttca
    14941 ttgagtatta aactaaatta aataatatta gcgcggagaa tttctaattc ttccttggtc
    15001 aagcgacgcc attcccctcg ttccaggttc tcatctaata ctaaagttcc catagtcaat
    15061 cgttgcaagt ccaccacttc cttgccacag tagcccacca tacgcttgat ctgatgaaac
    15121 ttcccttctg caatggtcac acggatttgg ctttgattct tttctgtatc tatggataca
    15181 agctccagta tagcgggttg acaggtaaag tctttgagag gaataccctc agcaaatgtc
    15241 tccacatctt cttgggtcat gattcccttg acttgtgcca gataagtctt gtccacatga
    15301 cgcttgggcg aaagaagaac atgagccagc tgaccatcat tggtcaagag caaaagacca
    15361 tgcgtgtcaa tatccaagcg tcctactggg aaaacttcct tactccgcgc caagtcatcc
    15421 aacaagtcca gaacggttct gtgcttggga tcctcagtcg ctgagataac tcctttgggc
    15481 ttgttcatca tgtagtagac aaactcttca tactccaaca cttgcccatc aaagcgaatc
    15541 tcatctattt tttcatcaat ctgcaattta gctgattttt cttttttacc atttacagtc
    15601 acgcgcccag ccttgagcaa gtttttgacc tcagtccgac ttcccaccgc acaggcaact
    15661 aaaaatttat ctaatctcat agaactatta tatcatatca aaaggaggct ggtacaatga
    15721 ccaacctcct tttcgtttca tactcttcaa aaatctcttc aaaccgcgtc aacgtcgcct
    15781 tgccgtatat atgttactga cttcgtcagt tctatctgca acctcaaagc agtgctttga
    15841 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatc agatttagga
    15901 aattaacttc ctcgtctcca aaaaatagct aaaacaatca tggctcctaa aatcgctggg
    15961 ataatagctg ttcctgataa aactggcccc caagttccaa agagcaagtg acctagaaag
    16021 gctccgatcc aaccgagaaa catttttcca aaacatccca ttcgctctcc acgattggtc
    16081 atagcacctg ctaaaaatcc cactaggaga ccaacgaaca tacttcctaa catattatct
    16141 ccttaatttg cccaattccc attacggaaa agaggtactc gcgttccatc ctcacgaata
    16201 ccatcgatat ccatttggtt agaaccaatc ataaagtcta cgtgaacatc tgaacggtta
    16261 agccctgcag cttcaagctc ctcttcgctc atctccgctc catcaacaac gctagtcgca
    16321 taggctgcac cgatagccaa gtggtttgac gcattttcat cgaaaagggt gttaaagaag
    16381 gtaatgcctg actgagaaat tggacttgga tctggtacca aggcacattc acccaaggca
    16441 cgcgcacccg cattttcaaa gacaaggtct ttcataacct gatcaccctt ctcagcagtg
    16501 atatctacga tttgtccatc cttaaaggtc accttaatgc cttcaatgat atttccgttg
    16561 tagctaagcg gttttgtaga agtgacataa ccatctgcac gacggaagtc aggcgctgtg
    16621 aagacctctt ctgtcggcat atttggcaag aattcttcgc cctgtgcatt gacagcacca
    16681 gctgattccc aaacgtggtt ctttggcaaa ccaagtgtta aatctgttcc tggcgctgtg
    16741 tagtgaaggg ctgaaaattg ctccttatta agcatattgg ccttgctctt gagaatggct
    16801 gcatgttcct cccaagcctt aacaggatct gcttcgtaga cacggcaagt tttgaaaatt
    16861 tggtcccaaa ggaaatcaac tgcttcttcg tcgctcgcag catttgggaa gactttctta
    16921 gcccactcaa gtcctgctgc agctgctaca gtccagctaa ccttgttaga ttgagttgcg
    16981 atacgcatag gcttcatggc aagtcccata gctttagcag aagctgaaag cttgtcagcg
    17041 tccactccgt tcaaggcacc cggatcagat gaacgaactc caagacggct agccttgttc
    17101 tccaagagat agttcatctc agcaatcttg tattctggca cattgtccaa acgctccatc
    17161 ggggcatgga ggaatttctc acggttaatc acatcatctg tccactgaac gatgacctca
    17221 tgcgcaccca aggcataagc ttctttcacg attagatgtg ccaattcacg ttgctccaca
    17281 tcaatagaga gagccaaagt gtgaccaggt tgcacgttaa ttccgttcgc aaccaacaat
    17341 ttcgcatatt tttctagatt ttctttaaaa tttggtaaaa ccattctgtt ttctcttttc
    17401 tatttttatt tttctctcaa agcagaaaag attcctgcta aagcaatagc acctgacaag
    17461 agtgctgtcg atagaagaat tgtacgatca gcaagttcta attgatattc aaataccttc
    17521 ttagccggct tatcttccgc aaatattctt agtttcatga gaaccccctt aaaatagatt
    17581 aaaataaatc actgtgactg cctgtcctca gcaaatttaa aatcaattcc gacttgtcta
    17641 ctttataaac caaaagccaa tctggctggg tatggcattc acgaactcct tgaaaatgct
    17701 tggatgccgt caatgaatga tcacgatatc tggcaggatg ttctttttct tgaaccagaa
    17761 aattcaaaac ttcttctaat aattctgcct tcaaaccacg cttcatagcc aacttaaaat
    17821 cttttttaaa ctgtttatga taacgaatct taagcacgta agtcctccat caaatctgag
    17881 actgattcaa aagactggct catattacga ttttggtcta aatccgttaa cgcttggagc
    17941 aacttccgat tttcgtctag tctaacatca aaaggtagtc cctgatattg aattgcctga
    18001 cgaaggaaaa tattaatagc tgttgtcata tccattccca gattactaaa cacctgttgg
    18061 gcctgctcct taacctcact atccagacgg atgcttatac tcatctttga catactctca
    18121 ccctctttcc atagactatt ttaacaaaaa agaaagctaa tgtaaatcta ttggatatac
    18181 gttagcctct tttaatagat tattaagcaa ttttttaaaa caactcatca aacaaactca
    18241 actggttatc ctctggcata tttccaagaa tacccatctc atccatcttt tcaaccaagg
    18301 ttgatgagag tccaccacgc ttgcgtagtt ctgttttaga gaggaattct ccctcttcac
    18361 gcgcccgcac cagttgcttg gcaacgttct ctcccagacc atccattgct acaaatggtg
    18421 ggataagggt atccccgtcg atgaggaact ctgtcgcctg actacagtag agatctaatt
    18481 taccaaactt gaaacctcgt tcccacatct cattgacaat ctcaagagtt gtatagagat
    18541 cgatttccac attagaggct tcattgttct tccgtttttc agagatttct tccattctgc
    18601 gcttgatggc ctccaagccc gcacccatgg tcttgatatc aaaagcctta gcacgaatgg
    18661 agaagtaagc acagtagtaa taaataggat ggtgaacctt gaagtaagct acacgcaagg
    18721 ccatcataac gtaggctgcc gcatgggcct tagggaacat gtacttaatt ttcccacagg
    18781 attcgatata ccactctggc accttattag ccttcatggc ttcgatatag ccatttctct
    18841 cctcttctga aatctttagc cacaaaccct tacgtacccg ttccataatg gtaaaggcca
    18901 tcttaggttc cagacccgca tgcatgaggt aaaccatgat gtcgtcccga caaccgataa
    18961 cagtcgatag gtccgctatt ccttgcttaa tcagatcctg agcattcccc aaccaaacgt
    19021 cagtaccgtg ggacagacca gacagctgaa gcaattccgc aaaggttgtc ggatgggttt
    19081 cgtctaccat tccacgtacg aaatttgttc caaactctgg aatccccaac atacccgtag
    19141 gcgttccaat ttgttcaggt gttaccccta gcacatcagt cccagaaaag agtgccatca
    19201 cgccttcgtc atccatagga attttattag ggtcaatacc agacaaatcc tgaagttttc
    19261 gaatcatggt cggatcatca tgtcccagta catcgagttt gaggacgttc tcatcgatat
    19321 cgtggaagtt aaagtgagtg gtctgccatt cagccgtgac atcatctgct ggatactgga
    19381 caggcgtaaa atcgtagaca tccatgtagt tcggaataac aacgattccc cccgggtgtt
    19441 ggcctgttgt ccgcttgaca cccgccgctc cttgagcgag gcgttctact tctgcatcac
    19501 gataaaactt gccataatct cgctcgtaac ccttgacaaa tccataggca gtcttggcag
    19561 ctaccgtacc aaccgttccc gcacggaagg catattcttc accaaagata tcacgcacat
    19621 ccaagtgggc gctaggctga tcttctcccg agaagttcaa gtcaatatca ggaaccttat
    19681 ccccatcaaa accaaggaag gtctcaaacg gaatatcctg tccgttttta ctgagtttgt
    19741 gaccacagtt tggacagtcc ttatggggca tatcaaatcc tgaaccgtac gaaccatctg
    19801 tgataaactc actgtactga cactgaccac agacatagtg aggagagaga ggattgacct
    19861 ccgtaatccc aatcatggtc gcaacgaaac tagatccgac agatccacga gaaccaacca
    19921 agtaaccccg ttcattagaa cgttgcacca gcatctgcga tgccagataa atcacagcaa
    19981 atccattccc cagtatggat gttaattctt tttcaatccg caaatcaaca atatctggca
    20041 gcggatttcc ataaatctca aaagctttct tataggtcaa ctcagcaact gtttcttcag
    20101 ccttgtcgat gaaaggcgta tacaagtcac ccttaacgac ttcaacgggt tcaaatattt
    20161 ctgccaaggc attggtgttt tcaataacca gtttacgagc cagttcctct cccaaaaagg
    20221 caaattcatc caacatctca ttagtcgttc gaaaatgagc ctttggaagt ggcgctggtt
    20281 gggcatgttc accatgaccg atagttcggt taatcatcgc accctgtccc aaactacgga
    20341 cgataatttc acgataaatc tcttcttccg gttcgatata gtgaacattt cccgtagcca
    20401 gaacaggctt gccaaggcga tctccaacct ctatcaaact cttgataatg gtctggagtt
    20461 cctccatatc cttgacctgc tctttggcaa tcaagggcgc atagatagcc ggtggcatga
    20521 cctcgataaa gtcataatac ttggccacct caaccgccgc atccacacct tgagaaacga
    20581 ccacgtcaaa aacttcaccc tctgaacagg ctgaacctaa aatcaagccc tctcgatggg
    20641 catctagaac cgttctcgga atccgtggca ctccttcaaa atacttggta ttagacaagg
    20701 aaaccagctt aaagatattt tttagaccta cctgattctt gacatagatg gtcgcatgct
    20761 tgatccgagc ttttttgtaa gaatctggac tgattagatc aatgttgagt ctagctaaat
    20821 cggtcacacc atgtttttct gctacctctt tgataaagat gaaaagcaga cgaccagtcg
    20881 cttccgcatc gtagttggcc atgtggtgat gttccaaggc cacaccaaaa cgcttggtca
    20941 aaggccccaa accatggcgt ttatactcag gatagaggtt tctagcaaac tccagcgtat
    21001 caataactgg ctgactaatc tttggaagac catgacgctc ataattagca ttcataaagc
    21061 caacgtcaaa ggcagcattg tgggcaacta ggaccgtatc cttgcaaaat tcttggaatt
    21121 cttgcaaaac ttgttctagt ggtttggcat ttttgacatg atcatctgta attccagtta
    21181 actctgtagt aaaagctgac aagggatgcc caggattgat aaattcatca aattcagcaa
    21241 taacattccc cttgtacatc ttagaggccg caacctgaat caagtcatta tagatagctg
    21301 aaagtcccgt cgtttccacg tcaaagacca cgtaggttgc ttctgataag tccatctcca
    21361 cttcgttata gacgataggg acacggtcct ccacgatatt ggcttccatc ccatagatca
    21421 gctggattcc cgctttctta gccgccttat agccatgtgg aaaggactgg acattcccat
    21481 ggtctgtgat agcaaccgcc ttgtgtcccc acttagcagc tgttgcaaca atctcttcga
    21541 cctctggcaa agcatccata gtcgacatgt tagtatgagc atgaaactca acccgacgct
    21601 caccttctgg catcaaatcc ttccgctcat agtgaacaac ttcctgcaga tcctgtacgt
    21661 tcatagtcaa atcgcgtgtg aagttattca tctccacatt ccctcgaact cggagccaag
    21721 aattcttctt gatgaggtca aacttctggg cctcttcctc gtttttaacc cacttttgca
    21781 tagaaaaact tgaagtatag tccgtcattt taaagttgat taaaacacga cctgttctag
    21841 tcactttttg ctccacatca aaaacaaccc cttcaaatac cagacgattt tcctctgtcg
    21901 tcacttcgat cataggagta atctccgcct tatccagctt gggtttagct gcagcttttt
    21961 tcgcttgaaa atcaaagact ggtttctctt ccactggagg aggtgccatc tgctccagtt
    22021 gttccatagc acggagcgct tcctcattgg cagcttgaac aatctgctca ttttcagcat
    22081 gaaaggcctc ttcctgctct tgggtcagga catcattctt ctcaacttga cagttaaaag
    22141 tttgaaaacc aaacttttca agttgcttgg ctaaattagg aagatgattc ttcttaaaat
    22201 gttccttatc aatcgcttca gatccttcaa taaatagctg attaccctca gcacgaactt
    22261 gcaaattttg ataaagggac ttaaaacctt gactagcaca tggaccttca gagaaagcct
    22321 ccctatagta ggactgcaag agctgatttg aaaattcttg agaccgagcc ttaatttcaa
    22381 aaacagcttt attgcctgtc ttagaaaatt cttcgctcaa acctttcttt aattctaaaa
    22441 agatttcaat cggtaaaata ttagaaaata cgaaatgaaa ctcccatacc ttactaattt
    22501 tatgaaccac aactcgctca atattggcct gtgctaaagc aggagcctgt ctcatttcag
    22561 caggcatccc caattgattc atcaaaattt caaaactatt tgacattcat tttcctcaca
    22621 ttattcttct actattttac catatttaga ggtattttct aaagacaaaa ggaagccact
    22681 aagtgacttc cttctagagt gaggacggat tagtcttcac ctttattttt cttaataatt
    22741 tcttcttgta ctgacttagg tacatcttcg tagtggtcaa ataccatcat gaatgtacca
    22801 cgtccttgag atgcagaacg aagaactgtt gcgtaaccga acatttcagc aagtggaacg
    22861 taagcacgaa cgatttggct gttaccgtgt gcttccatac catctacacg tccacgacga
    22921 gcagttacgt gacccataac atcaccaagg ttttcttctg gaacagtgat tgttacaagc
    22981 atcattggtt caaggatagc tggttgtgct gatttagcag cttctttaag ggaaagtgaa
    23041 gccgcaatct tgaaggcagt ttcagatgag tcgacatcgt gatatgaacc atcataaagc
    23101 ttagctttaa cgtcaaccat tgggtaacct gcaagaacac cgttagccat agattctacc
    23161 aaaccttttt caaccgctgg gataaattca cgaggaacca caccaccgac gattgcgttt
    23221 tcgaattcga atcctttacc ttcttcgttt ggagtaaatt caatccacac atcaccgaat
    23281 tgacctttac caccagactg acgtttgaag aatccacgtg cttgagtaga agcgcggaat
    23341 gtttcacggt aagatacttg aggagcacct acgttcgctt caactttgaa ctcacgacgc
    23401 atacgatcaa caaggacgtc aaggtgaagt tcacccatac ctgagataac tgtttcacca
    23461 gtttcaacgt ttgtttcaac gcggaatgtt ggatcttctt cagccaattt ttgaagggcg
    23521 atacccatct tgtcttggtc agctttagat tttggctcaa ccatcaattg gataactggt
    23581 tctggaacgt tgattgactc aaggatgatt ttagattttt catctgtcaa tgagtcacca
    23641 gttgtagtat ctttcaaacc aacggcagca gcgatatcac ctgagtaaac agtgtcgatt
    23701 tcttgacggc tgttagcgtg catttgaagg atacgtccga tacgttcacg tttaccttta
    23761 gaagtattca atacgtatga acctgattga agaacacctg agtaaacacg gaagaatgtc
    23821 aaacgaccta cgaatgggtc agtcatgatc ttgaaggcaa gagctgcaaa tggctcttcg
    23881 tcagatgctg gacgagtttc ttcagcgtct gtatctgggt taataccttt gattgctggg
    23941 atgtcaagtg gacttggaag gtagtcgata accgcatcaa gcatcaattg aacacctttg
    24001 tttttgaagg ctgaaccaca caatactggg aagaattcaa cgttgatagt cgctttacgg
    24061 ataccagctt tcaattcttc gttagtgatt tcttcacctt cgaggtattt catcatcaat
    24121 tcttcgtcag tttcagcaac tgcttcaatc aatttttcac ggtattcttg agcttggtca
    24181 aggtattcag ctgggatgtc ttcttcaagg atatccgtac caaggtcgtt agtatagatt
    24241 tcagctttca tcttgatcaa gtcaatgata ccacggaagt catcttcaga accgattggc
    24301 aattggattg ggtgtgcatt tgcttgaaga cgatcgtgaa gtgtgcttac agagtaaagg
    24361 aagtcagcac cgattttgtc cattttgttg gcaaatacga tacgtggaac tccgtactca
    24421 gttgcttgac gccaaactgt ttcagtttga ggttcaacac ctgattgtga gtcaagaacg
    24481 gtaaccgcac catccaatac acgaagagaa cgttgtactt cgattgtgaa gtccacgtgt
    24541 cctggtgtgt cgatgatgtt tacgcggtgg ttgttccatt gagctgttgt cgcagcagat
    24601 gtgatcgtga taccacgttc ttgctcttgc tccatccagt ccatttgtga cgcaccttcg
    24661 tgagtttcac cgattttgtg gattttacca gtgtagtaaa gaatacgctc agtagttgtt
    24721 gttttaccgg catcgacgtg agccatgata ccgatattac gagttttttc aagtgaaaat
    24781 tcgcgtgcca tgaggtttgt ttctcctatt tatttttgat ttctattcta ttataacacg
    24841 attttaataa aaacggatag gcaggaccta cccgttctca atgttttcat gctattgttg
    24901 gtttcaactt acgagatggt aagctgagtt atagctaata ctaatcgatt tagctaattt
    24961 gaacccgggc taaagttagt tagccgatgt gagctggaac gggatgctgc gtgaaaaaga
    25021 taaaactcct tgtattcgtt gaatactgcg tcagtttcct attttcacct tgcatcctta
    25081 ccgtgcctag catcatgatt tagttgagct caagctaaaa gtctggagaa aaagatgaat
    25141 ctcattggtt gcaaccgcaa cctgcttcga ttccctattt tctctgggac tttcttaacg
    25201 ctttcgcatc ctatcttacc aacggaagtg tgcgaatgca cggttagctt cagccatacg
    25261 gtgagtatct tcacgtttct taacagctgc accagtgttg ttagcagcat ccaagatttc
    25321 ttttgcaaga cggtcttgca ttgtgtgttc accacgaaga cgagcgattg ttaccaacca
    25381 acgaagtcca agtgttgtac gacgttctgg acgaacttca actgggactt ggtagttaga
    25441 accaccaaca cgacgtgcac gtacttcaag tacaggcatg atgttttcca tagctgtttc
    25501 aaatacttca agtgcatcgt tgccagtagc ttctttgatt tgctcaaaag caccgtaaac
    25561 gattgaagca gcagtaccac gtttaccatc aagcataacg cggttgataa gacgagtaac
    25621 tagttgtgaa ttgtatagcg gatctggcaa tacgtcacgt tttggagctc tatttttacg
    25681 actcatttct ctttatcccc tttccttatg cttttggacg tttagtaccg tatttagaac
    25741 ggccttgttt acgatcgtta acacctgcag tatcaagtgc accacggacg atatggtaac
    25801 gtacccctgg aaggtctttt acacgtccac cgcgaagaag caccacgctg tgctcttgca
    25861 agttgtgtcc gatacctggg atgtaggcag taacttcgat aaggttgctc aaacgtacac
    25921 gagcgaattt acgaagggct gagttaggtt ttttaggtgt cattgttcca acacgagttg
    25981 caacaccacg tttttgtggt gaagaaacgt ttgtttgaac ttttttatga ctattgtaac
    26041 caacgttcaa agctggtgat ttagattttt ctacttttga tttacgcggt ttgcgaacca
    26101 attggttaat tgtaggcatc tacattctcc tgtgtttttt tatttttggt gatgatacac
    26161 ttggtgacag atatcatctg tgtgtacttt tgcaacattt gtcagcacgt ccctgtacac
    26221 tcttgagaga ccaaaagtaa aaagtaccgt ctattattgt aacaaaattt tcccttgctt
    26281 gtcaagatat ttttcttttt tattggtaat gttagagatc aatttctgaa taaaaatagc
    26341 tgggaacaca tcatcttcct aataatacgg cttggcttat gtgacatttt tatctccgtc
    26401 atttatactg atatataaaa tagtacagaa aacttctcaa aagtaagaat ttttttcaca
    26461 aatcaaagaa aacgcttgca tattgtttta ctacatgcta tactgatata gaaatttatt
    26521 gattggagaa tcattatgag aaaaacacca tctcacactg agaaaaaaat ggtctacagc
    26581 atccgttccc tcaaaaatgg aactggttct atccttatcg gcgcaagcct tgtcttgctt
    26641 gccatggcta caccaactat ctcatccgac gaaagtacac caaccactaa cgaacccaac
    26701 aacagaaata caaccaccct tgcccaacct cttactgata cagcagctgg ctctggtaag
    26761 aacgaaagtg atatttcttc acctagaaat gcaaacgctt ccctagagaa aacagaagaa
    26821 aaacctgcaa cagagccaac tacttctact agtccagtaa caactgaaac taaggcagaa
    26881 gagcccatcg aagataacta cttccgtatc catgtcaaaa aacttcctga agaaaacaag
    26941 gatgctcaag gactatggac ttgggacgat gttgaaaaac catctgaaaa ctggccaaac
    27001 ggagctttgt ccttcaagga tgccaagaaa gatgactacg gctattacct agatgtcaaa
    27061 ttaaagggag aacaagccaa gaaaattagc ttcctcatca acaatacagc tggaaaaaat
    27121 ctaaccggcg ataaatctgt agaaaaacta gttccaaaaa tgaacgaggc ttggttagac
    27181 caagattaca aggttttctc ttacgagcca cagcctgcag gaactgttcg cgtcaactac
    27241 taccgcacag atggcaacta tgacaagaaa tctctctggt actggggaga tgtaaaaaat
    27301 ccaagtagcg ctcaatggcc tgacggaaca gactttacgg ctacaggcaa atatggccgc
    27361 tatatcgaca ttcctcttaa tgaagccgca agagaatttg gatttttatt actagatgag
    27421 agcaaacaag gagacgacgt gaaaatccgt aaagaaaatt ataagttcac agatttgaaa
    27481 aatcatagcc aaattttcct aaaagacgat gatgaatcga tttacacaaa tccatactat
    27541 gtccatgata tccgtatgac aggagcccaa cacgtaggca cttctagcat tgaaagtagc
    27601 ttttcaacac ttgtcggtgc taaaaaagaa gatatcctca aacactccaa catcactaat
    27661 cacctaggaa acaaggtaac tattaccgat gttgcaatcg atgaagctgg taagaaagtg
    27721 acctacagcg gagatttctc tgacacaaaa catccttata ctgttagcta caattccgac
    27781 caattcacta ccaaaacaag ctggcacctg aaagatgaga catacagcta tgatggcaaa
    27841 ctgggagctg acctaaaaga agaaggaaaa caagttgatt tgaccctttg gtcaccaagt
    27901 gctgataagg tttctgttgt tgtctacgac aagaatgacc ctgacaaagt agttggaact
    27961 gtcgctctcg aaaaagggga aagaggaact tggaaacaaa ctctagacag cacaaacaaa
    28021 ctcggaatca cagatttcac tggctactat tatcaatacc aaatcgagcg tcaaggtaaa
    28081 actgttcttg cactcgatcc ttacgctaaa tctcttgctg cttggaatag cgacgatgcc
    28141 aaaattgacg atgcccataa agtggctaaa gccgcctttg tagatccagc taaactcgga
    28201 cctcaagact tgacttatgg taagattcat aatttcaaga ctcgtgaaga cgccgttatc
    28261 tacgaagctc atgtgcgtga cttcacttca gatcctgcca ttgcaaaaga cttgaccaaa
    28321 ccatttggga cttttgaagc cttcattgaa aaactagact atctcaaaga cttgggtgta
    28381 acccatatcc agctccttcc agtcttgtct tactactttg tcaatgaatt gaaaaaccat
    28441 gaacgcttgt ctgactacgc ttcaagcaac agcaactaca actggggata tgaccctcaa
    28501 aactacttct ccttgactgg tatgtactca agcgatccta agaatccaga aaaacgaatc
    28561 gcagaattta aaaacctcat caacgaaatc cacaaacgtg gtatgggagc tatcctagat
    28621 gtcgtttata accacacagc caaagtcgat atctttgaag acctagagcc aaactactac
    28681 cactttatgg atgccgatgg cacacctcga actagctttg gtggtggacg cttgggaaca
    28741 acccaccata tgaccaaacg gctcctagtt gactctatca aatacctagt tgatacctac
    28801 aaagtggatg gattccgttt cgatatgatg ggagaccatg acgccgcttc tatcgaagaa
    28861 gcttacaagg ctgcacgcgc cctcaatcca aacctcatca tgcttggtga aggttggaga
    28921 acctatgccg gtgatgaaaa catgcctact aaagctgctg accaagattg gatgaaacat
    28981 accgatactg tcgctgtctt ttcagatgac atccgtaaca acctcaaatc tggttatcca
    29041 aacgaaggtc aacctgcctt tatcacaggt ggcaagcgtg atgtcaacac catctttaaa
    29101 aatctcattg ctcaaccaac taactttgaa gctgacagcc ctggagatgt catccaatac
    29161 atagcagccc atgataactt gaccctcttt gacatcattg cccagtctat caaaaaagac
    29221 ccaagcaagg ctgagaacta tgctgaaatc caccgtcgtt tacgacttgg aaatctcatg
    29281 gtcttgacag ctcaaggaac tccatttatc cactccggtc aggaatatgg acgtactaaa
    29341 caattccgtg acccagccta caagactcca gtagcagagg ataaggttcc aaacaaatct
    29401 cacttgttgc gtgataagga cggcaaccca tttgactatc cttacttcat ccatgattct
    29461 tacgattcta gcgacgcagt caacaagttt gactggacta aggctacaga tggtaaagct
    29521 tatcctgaaa atgtcaagag ccgtgactat atgaaaggtt tgattgccct tcgtcaatct
    29581 acagatgcct tccgacttaa gagtcttcaa gatatcaaag accgtgtcca cctcatcact
    29641 gtcccaggcc aaaatgatgt ggaaaaagag gatgtagtga ttggctacca aatcactgct
    29701 ccaaacggcg atatctacgc agtctttgtc aatgcggatg aaaaagctcg cgaatttaat
    29761 ttgggaactg cctttgcaca tctaagaaat gcggaagttt tggcagatga aaaccaagca
    29821 ggatcagtcg gaattgccaa cccgaaagga cttgaatgga ctgaaaaagg cttgaaattg
    29881 aatgccctta cagctactgt tcttcgagtc tctcaaaatg gaactagcca tgagtcaact
    29941 gcagaagaga aaccagactc aaccccttcc aagcctgaac atcaaaatga agcttctcac
    30001 cctgcacatc aagacccagc tccagaagct agacctgatt ctactaaacc agatgccaaa
    30061 gtagctgatg cggaaaataa acctagccaa gctacagctg attcacaagc tgaacaacca
    30121 gcacaagaag cacaagcatc atctgtaaaa gaagcggttc gaaacgaatc ggtagaaaac
    30181 tctagcaagg aaaatatacc tgcaacccca gataaacaag ctgaacttcc aaatacagga
    30241 atcaaaaacg aaaacaaact cctatttgca ggaatcagcc tccttgcgct ccttggtctc
    30301 ggtttcttac taaaaaataa aaaagagaac taaactagcc ctcctataga aaaatccccc
    30361 aagcattata gctcggggga ttaatttttg tacaatattt gttgtcctaa taaacttgat
    30421 taggattttt tattaagcct ctttcatagc aaaataagct cgtactttgg gtgcaacttg
    30481 tgttccgaat agttcaatag ctctcagaac ctggtcatga ggcatagaac caagcggtag
    30541 atgaagcatg aagcggtcca atcctaaatc ctctatcatg cgaatcaatt tttcggccac
    30601 ctgatctgga ttgccaacaa acatggcgcc atttggccct acctgctcca aatattgctc
    30661 ataacgcaat tcctgccagt gcggacggtc tttggaaata gcatccacca cttgcttagt
    30721 cggatggaaa taatctttca ccgcctgctc accatcttcc gcaatccacc cccaagaatg
    30781 ggctcccact ttcaagtctt tgtcagcatg gcccgcttcg cttccaatct cacgataagc
    30841 ctgaatcaac tttttaaaat aacgtggatt accaccaata atagcatata caatcggtag
    30901 accagcctga gcaatcttca ctgttgattc gacatgacca cctgtagcta tccacaaggg
    30961 caatttgtcc tgaactggac gaggataaac ttctttacca gcaatcgttt gagtcaatcg
    31021 accttgccag tctaacttgg tcttttcatt gactaactga agcaagtcta atttctcatc
    31081 aaaaagagag tcgtagtctt tcaagtcata accaaacaga gggaaagatt ccgtgaaaga
    31141 gccccttcca gccataatct ccgatcgtcc atttgacaaa gcatcgatag tggcatactg
    31201 ttggaacaaa cgaatcgggt ccatgcttga cagaatgctg actgcactgg tcaaacggat
    31261 tttcttggta ttgactgccc cagcggccag gacaatctct ggggctgata ctgcaaaatc
    31321 cgcccgatgg tgctcgccaa ttccatatac gtccaaacca accttgtcag ccagctcaat
    31381 ctctgccacc aactggcgaa tgcgttcagc atgactgtaa gtttgtccag tcccttcaag
    31441 ctccgttatt tccccaaatg ttgaaattcc caattctacc attgtgattc tccttgtcta
    31501 tctctgtcct tcaatttgaa aaattattct aatactcaat gaaaatcaaa gagcaaacta
    31561 ggaagctagc tgcaggctgc tcaaaacagt gttttgaggt tgtggataga actgacgaag
    31621 tcagtaacca tacctacggc aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt
    31681 ataacacgaa tcttgagtac aagcaaccaa tttgctcaca agaaaaagcc tagataacta
    31741 gactttttta gcttattcta ccgttactga cttggcaagg ttacgtggtt tgtccacatc
    31801 gaggccacga tggagggttg caaagtaagc gactaattgc gttggtacga ccattgaaat
    31861 tggtgagagg tatggatgta cggtcgtaag gacgatatcg tcggtatctt tggctacatt
    31921 ctcttctgcg atagtgagga ctttggcacc acgggctgcg acctcttgga tatttccacg
    31981 agtatggttg gcaagaactg gatctgacaa gagagccaaa acaggcgttc cttcttcaat
    32041 caaggcaatg gttccgtgct tgagttctcc tgcagcaaag ccttcacact ggatataaga
    32101 aatctctttg agtttgagac ttgcttccat ggctacgtag taatcttgac cacgtccgat
    32161 gtaaaaggcg ttacgagttg tttcaagaag ttcacgaacc ttgacttcaa tggtttcttt
    32221 ctctgaaaga gttgattcga tagactgagc tacgattgac aattcatgaa ccagatcaaa
    32281 gacttgcgct ttagcattac catttgcttc tccgactgct tttgcaagga aggcaagggc
    32341 tgcgatttgc gctgtatagg ctttagttga tgccacggca atttcaggac ctgcgtgaag
    32401 gagcatggta tagttggctt cacgtgagag ggttgaacct ggaacgtttg tcactgttaa
    32461 gcttggaatt cccatttcat tagccttgac caaaacttga cgactatccg ctgtttcacc
    32521 agattggctg ataaagatga agagtggttt cttgctgaga agtggcatac cgtagcccca
    32581 ctcagatgag attccaagtt caactggtgt atctgtcaat tcttccaaca ttttcttaga
    32641 agcaaatcct gcatggtaag atgttccagc tgcaaggatg tagatgcggt ctgcgtcttg
    32701 aacagcctta atgatatctg ggtctacgac aacttgacca gcctcatctg tgtaggcttg
    32761 gatgagtttc cgcataacag ttggttgctc gtcaatttcc ttgagcatgt agtaagggta
    32821 agttccctta ccgatatctg acaagtcaag ttcagcagtg tagctagcac gctcacgacg
    32881 atttccatca tagtcttgaa cttccacact atcagccttg acgattacca actcttggtc
    32941 atggatttcc atgtattggt tagtttcacg aatcatagcc atggcgtctg agcagaccat
    33001 gttatagcct tctccaagac caatcaaaag tggtgattta tttttagcta cgtagatgac
    33061 ttcaggatct tgtgagtcaa ccaaggcaaa ggcataagaa ccacggatga tgtgaagggc
    33121 ttttttgaag gcttcaagaa ctgagagccc ttcttcttcc gcaaattttc caatcaaatg
    33181 aacggctatt tcagtatctg tctgcccctt gaagtggtga cctgcaaggt attcttcctt
    33241 gatttcaaga tagttctcaa tcaccccatt atgcaccaag acaaaacgtt ctgtcttaga
    33301 gcggtgtggg tgagcattgt cctcagttgg ttttccgtga gtagcccaac gagtatgtcc
    33361 gataccagtt gttccctcaa caccagctgt cttggcagac aattctgcaa tacgaccaac
    33421 cgccttcacc aaatggttat cagcaccatc taggacaaaa attcccgcag aatcatagcc
    33481 acggtattca agcttttcaa gcccttgaat caaaatatca gttgcatttg tgtttccaac
    33541 aacaccaaca attccacaca tagtattttc ctttttaaaa ttggtatagt ctaattaacc
    33601 aaattggcaa tcagtgaagt tattataaaa cttgtttttt aaattgtcaa gaaaaaattg
    33661 gtatagttca aattaagctc ctgtaagcat aaaaactctg accgattggg ataatcagtc
    33721 agagtccttt ttaaaatcca ttattatcgc ttaattcttt gaaccagtgg cctgatttct
    33781 tcagacgacg ttcttgcgtt tccaagtcta attcgaccaa accataacga tttttatagc
    33841 tgttgagcca tgaccagcag tcaataaagg tccaaatcaa gtagccctta cagttggcac
    33901 catcttcaat ggcacggtga agttcacgaa gatgaccttt tacaaagtca atacggtaat
    33961 catcttgaat cattccatct tgacggaatt tttcttcccc ttcaacaccc ataccattct
    34021 cagtcaacat ccactcaata ttgccataat tttccttgat attttgggcg atgtcataaa
    34081 tcccttgctc ataaatctcc caaccacggt gagaattgat tttacgtcca ggcatcacat
    34141 aaggctcgta aaaatgttct ggtaagagtg gactctctgg atgcttagca aatcgaggag
    34201 ccataacacg caaaggttga tagtagttca caccaaggaa gtccaccgta ttatcacgaa
    34261 tgagttccaa ctcttcctct gtagcatcag gtaaaagacc gtgttcatgc aagatttcta
    34321 ccaactcctg tggataagtc cccaagacag atggatctaa gaaagattgg gcctgaaaaa
    34381 gggccgcaat acgagctgcc ttgacatcag caggatgctg gctacgtgga taagccggtg
    34441 tcaagttgag gacaatccca atcttggaat caggcaaaag ttcatggcaa gccttaacag
    34501 cccggctgct ggccaattgt gtatgatagg ctaccttaac agctgcctct gcatccacct
    34561 tatgtggata atgggcatca taaaaataac caaattctac aggaacgatg ggctcgttaa
    34621 aggtaatcca ttgatccact aaatctccat aagtctcaaa acaaaaacga gcatagtctt
    34681 cataggctga gactgtcgcc ttattttccc aaccatcacc atcctcttga agggcaaaag
    34741 gtaaatcaaa atgatagaga ttgactaaca gacgaattcc tttagcctta atagcctcaa
    34801 agaccttacg ataaaaatcc acaccttgag ggttgacttt tccacagcct tgtggaaaaa
    34861 tccgtgacca ctgaatagaa gtccgaaaga ctgtgtgacc agtctctaac aaaagctcaa
    34921 tatcccgctc ccaattttca taaaaagtcg atgtcttatc tgaaccaatc ccattatagt
    34981 aacgatttgg ctccacttgg aaccagtaat cccagagatt gtctccctta ccgtcaccag
    35041 ctacacgtcc ttctgtctgc ggtccagaag tagaggatcc ccagacaaaa tcctttggaa
    35101 atcttagcat aacatttacc tctttatcta ctcatttctc ccattataca gaaaaaacaa
    35161 ggtaaaaact agttacattt tttccttgtt tttcttctga ttatagtttt tatttcttgc
    35221 ttaggatttc aagcgtttca agcacgttat ctgcatgaac ctcaatggtg tcaccagttg
    35281 ccttgatctt aacttctaca atgccatcgg ccgctttttt accaacagtg atacggattg
    35341 gaagaccaat caagtcacta tcgctaaatt taacaccgac acgttcgtta cggtcatctg
    35401 tcaagacttc ataaccagct cccatcaagc ttgcttcaag tttttctgtc aaggcttgcg
    35461 cttcttcatc cttgacattg acagtaatca aatgcacatc aaatggtgcc aattctttag
    35521 ggaaattgat tccccaagcg taacggtatt caccttttgg cgttttgtta acaaagaggc
    35581 gagcgtgttg ctccatcact gctgaaagaa gacggctgac accgataccg taacatccca
    35641 tgatgattgg cacagcacga ccattttcat ccaagacatc tgctcccatg cttgctgagt
    35701 agcgagttcc gagtttgaaa atatgaccga tctcaatacc acgcgcaaag ttaaggacac
    35761 cttgtccatc tggggaaatt tcaccctcac gaacttcacg gatatccaca tattctgcag
    35821 taaaatcacg gcctgggttc acaccagtca agtggtagcc atcttcgtta gcaccgacaa
    35881 ctgcattgcg aacatcttgc accttacgat ctgcaataat tttaatattc tctggcaaac
    35941 caactggtcc aagtgaacca aatcctgctt gaacaacatt cgccacttct tcttcgctag
    36001 caacgtcaaa gaaatctgct cccaagtgat ttttcaactt gacttcgttg agttggtcat
    36061 ttccaactag aagggctgca acaagctcac catctgcaat gtagaagagg gttttaatcg
    36121 tttgttcttc tggaacattg aggaaggctg caacttcatc aattgattta acatctggcg
    36181 ttgcaacacg agtaacttct tcttcagcga caacacggtt gcttggtttg tactcgtttg
    36241 ttgccatttc taagttagct gcatagctag actcacttga gtaagcaatg gtatcttcac
    36301 cagagactat ccatttgagc aattctgcct tgatttcttc ttgcacttct gcaggaattt
    36361 cgtcaaatga ggcaactgac ttgtccaaga caacccagcg gtcaaggtct gtacgagcag
    36421 atgtaatggc cataaattct tggctatcct taccacccat ggctccaccg tcaccaataa
    36481 tagccttgaa gtctaaacca ctacgagtga aaatacgctc ataggctgct ttgtactcat
    36541 cataaacact atccaaacta tcatagttag cgtggaaact ataagcatct ttcatgataa
    36601 actcacgtgt acgaagaagt ccattacgcg ggcgtttttc atcacgatac ttgggctgaa
    36661 tttgataaag gttgagtggc aattgcttgt aagatttaac agaatcacgg acaatagctg
    36721 taaaggtttc ttcgtgagtt ggacctaaga taaagtctga tttttcacgg ttttttagtt
    36781 tgtaaaggtc ttcaccatag gtttcgtaac gacctgattc acgccacaat tctgcactaa
    36841 gaagggcagg agccaacatc tcaacagcac caatcttttc gaattcttgg cgcatgatgt
    36901 ttttagcttt ttcaatcaca cggttggcaa gtggtagata agaataaaca cctgctgaaa
    36961 cttggcgaac ataaccagca cgcaacataa gagcatggct gataacttga gcatcgcttg
    37021 gcatttcgcg aagcgttggg ataggcattt tactttgttt cataatattc ctcgattatc
    37081 taaaaaagag tcgcataatg tcattccaag tcacagcaat catcaagaca accatgatga
    37141 ccactccggc caaggtgaca taggtttcaa tttcttgttt caatggtttg cgacggatgg
    37201 cttctaggat attgagcaca atcttaccac catccaaggc tggaatcgga ataagattaa
    37261 aaatcccaat attgatggaa atcattgcca agaagtacaa gatattctca attccatttt
    37321 tagcagcatc actacttgcc ttaaagatag caacaggtcc acccaacttg ttcaaatccg
    37381 gttggaaaat cagatttttc agagctgaga gaattcggag agctgagtca gcagcagttg
    37441 taaaaccacc tacaaacatg gatagaaaat ctgacttaac ccccggttga acacctagaa
    37501 ggtaacgacc ttgactatct tcgggtgtaa cagtgacttg tttgtcactc cccttttcag
    37561 aaatagtcac atccaaagtc ggtgccgtct tatctttggt ttctgtttcc acagcttgga
    37621 tcaagctttc ccagttgcta acctcatgtg agccaatctt ggtaatttgt gccgtttctg
    37681 gtactcctac cttggccaag gcaccttggg gcatgatatg gaactgattg gtatcaacat
    37741 ctctgacacc accctgcata aagattaaaa cccaaaaaac aacgacacct aagataaaat
    37801 tgttcatagg acctgcaaaa ttggtaatca gtttgcccca gatagtcgca ttttgatatt
    37861 gaacatctaa aggcgcaatc cgaacctcag taccatctgc ttccacaacc gttgcatcgt
    37921 gatccactgc aaatgttttt tcttcttcca gaaccaatcc tttgataaag agcttgtctt
    37981 caaaatcaaa ctgggtcacc tgcataggga gggctgtttg atccaatttt ttacctgaga
    38041 gattgatgcg tttaacctta ccatcatcag caagtgtcaa actaacaggc gttcctgtct
    38101 tgatttcagt tgtatcatca ccccaaccgg ccatgcggac atagccaccc agaggcaaga
    38161 ttcgaatggt ataggccgtt ccatccttgc caatgtgagc aaaaatttta ggtcccatac
    38221 cgatggcaaa ttcacgtact aaaatccctg atttcttggc aaagtagaag tggccgaact
    38281 cgtgcaccac tacaataatc ccgaaaacca gaataaaggt taaaattccg agcatagcgt
    38341 ttcctccgtc ttttgattaa aagagtccaa ataagtgcat gattggaaat acaagcaaca
    38401 tactatcgaa acgatccaaa acaccaccat gtccagggat aaatttccca gaatccttaa
    38461 caccaaaatg acgtttgatc gaactttcta gtaaatcacc aaattgtcca gcaatgctaa
    38521 agaaaatagc aaagactgac atcttgtaaa ttccatatgg aagagcaact gtactgtcaa
    38581 ctatcataaa gataatggtt actaaaattg ctcctaaaat accacccaag gcaccctcaa
    38641 gggttttatt aggcgatacc cttggtgcta actttcgttt cccatagttc atcccaacaa
    38701 gataggcacc actgtctgtc gcccagacga tacacaaggc taagagagcc ttgtccaaac
    38761 ctgcaacacg agcatctagt aaagcattaa atccaaagcc cacgtagaag ctcatagcaa
    38821 gagggaaaac cgcatcctca atcgtataag acttgctaaa aacggtcgtt cctaacatga
    38881 ttgaaatcaa aacactatag gcaaccacat tcccatcaac tggcaaaaaa gtcaggtaat
    38941 tctccaaggg aatggtcaat gcaaaggttg caaagagggt caagaggccc tccatcgtca
    39001 tggtctctag acctctcatc ttcaaaagtt catgcatggc tagcatggct atgattccga
    39061 ttgctatctg aagcaagagg cccccaatca ttaaaattgg taggaaaata gccagggcaa
    39121 tccctgcaaa caaggttctt ttctgtaaat cctgggtcat atttcctcct aaactcctcc
    39181 aaatcggcga tgacgacgat tataggcaag aatagcttcc tgcaaggccg cttcgtcaaa
    39241 atcaggccat aaggtgtccg taaaataaag ctcactatag gctccctgcc atggaaggaa
    39301 attgctcaaa cgtaattctc cactagtacg gataatcaag tctgggtctc gtaagtcctt
    39361 aggtaaatgc tgagtaaaga gatagttacc aatcaattcc tctgtgatgt cacctgggtt
    39421 gattttggca tctaaaacat cctgggaaat caacttaagc gcctgtgtaa tctcagcacg
    39481 tccaccatag ttaagagcaa aattaagaat caatcctgtg ttgttcttag tcaattcctc
    39541 agccttggtt aaagcttcaa aggtttgctt aggcaggcag tctgtctccc caatcatttg
    39601 aatcttaaca ttattcgcat gtagttccgg gacataatta tcataaaact ctactggcaa
    39661 gttcatgata aacttgactt cctgatctgg acgggtccag ttttccgtag aaaaagcata
    39721 gaccgtaata accttgacgc ccagtttgtt ggctgccttg gtcacggttt gcaatgcttc
    39781 catgcccgct ttgtgtccaa aaactcgcgg ttgcatacgt tttttagccc aacggccatt
    39841 gccatccatg atgatgccga tatgagcagg aacctgtgtc ggaacctcta cttccacagc
    39901 cttatctttc ttaaaaaatc caaacatgat cttattccta ttcaaaaatc tatcgtttca
    39961 ttataccata tttccccatt ttcttctatc actaagctat ttattctcag gcaccatacc
    40021 catttttcaa aaaaataagc cgcctgattg ggcgacttta tttttatagg gagattatta
    40081 tgaaaaagtt ttaggagttt aagttaaggt cttcttaact tatgaactta gtgtacactc
    40141 cctagcttaa agtttcctta agtattttta aaaatcaaat ttttccattt ctcctgccaa
    40201 tttttcttgg ataaacgtgt ttgatagagt tccattcggt cttcattttc taagaaatga
    40261 ggagttggac gaacttgaaa attcaaaata tcctccaaac cataaggtat atagagttca
    40321 aaatctgatt cttcattcaa gcgcagtcca actgccgtac accgttctgg atacttactc
    40381 atagcatcac gagaactggt ataggaagca gtgtgaggac tgtgctgatg catatagacc
    40441 tgatttttca attcccactg gtactgagga aagtcctctc gcagtttttt ctccagtaat
    40501 aaggtttcct cataagaaat atccggatca aagaaaatca cacctatatc tgtttcatga
    40561 tcaaaagggg atttgtctga caaaagattc caaatgaaat ttctgacaga acctgctgcc
    40621 aaccacgagt ctttcagacc aaggtctcgg atgatcgtca gaatggccat catatctgga
    40681 ttttctctaa aagcctctaa gatttcttgc ttatttttca ctgtattcat aacctaagtg
    40741 ctcatatgcc ttagcagtcg ccacccgtcc agaccgtgtc cgcatgataa aacctttttg
    40801 aatcaagtaa ggctcataca tatcttcaac tgtctcacgc tcttcggcga tattcacaga
    40861 aagagttcct agaccaacag gtcctccact gtacatctca atcatggtac gaaggatttt
    40921 ttgatccaca tagtccaaac cttcatggtc aacatccagc atagtcaaag ccttatcggt
    40981 aataatatca tcaattaccc cattccccat tatctgggca aaatcgcgca cgcgcttgag
    41041 gagacgattg gcaatacgag gggtcccacg actacgtagg gccaactcag atgctgcctc
    41101 atgagtgatt tccatctcaa aaatatctgc cgtccgctcg acaatttctg tcaagtcagc
    41161 atgggcataa tactccatat gacctgtaat cccaaaacgt gcccgtagcg gattggagag
    41221 cataccagcc cgagtcgtcg caccaatcaa ggtaaaaggt ggtaactcca aatgaacact
    41281 acgactaccc tcaccagccc caatcataat atctatgtag aagtcctcca tagcactata
    41341 aagcacctct tccactgaca ttggcaaacg atggatctca tcaataaaaa ggacatcccc
    41401 aggctctaac tcattcaaaa tagctaccag atctccggct ttttcaatga ctggacccga
    41461 cgtttgcttg agattgactc ccagttcatt ggcaataaca aaggccatgg tcgttttccc
    41521 gagacctgga ggcccaaata agagcacatg atccagcgct tcatcccgca tttttgcagc
    41581 ttcaataaag atttgaagct ggtccttaac cttatcctgc ccaatatatt cacgtaagta
    41641 ctgaggacgg agcgtgcgtt ctactaactc ctcgtccccc attatctcat tatctaaaat
    41701 tctactcatg gctctattat atcaaaaaac caagccacaa acaaaaaagt cactggattg
    41761 gtgcttcccg agtttagcac ttatgtggta taatattata cggcacttct acaccgccta
    41821 cgaaaggagg tgagatagcc catgatggaa ttagtactca aaactatcat cgaaccaatt
    41881 gtggtcggtg tcgttcttcg attagtcgat aaatggctaa acaaggacaa atagtgtcaa
    41941 aaaagacctc aagcttattt ggtcgtgagc ttggggtctt ttctagccta tgatatagaa
    42001 ctagtactca attccttttt attatcccat agttcacaca ttttgtcaaa gttttatatt
    42061 ttcatccatt cagcttctcc aactttagaa tctgtccaat aaagtttata agattaagtg
    42121 aaagttatcg gaaaatgatg tttaaaatag tccccaaaag cctgaaatag agcaaaaaac
    42181 tccacctggt tgggtgactc ccgagtttag cacttatgtg gtataatatt ctacggcact
    42241 gctacaccgc ttgcgaaagg aggtgagata gcccatgatg gaactaatcc ttaaaactat
    42301 catcggaccg attgtggtcg gtgtcatcct tcgcttagtc gataaatggc taaacaaaga
    42361 tagatagtgt caaaaaagac cccaagctta atgtggaagt tagcttgggg tcttttctag
    42421 tccatgatat agaactaatc cttaattcct ctccattatc ccacagttca caaaatttgt
    42481 caaaacttta catcctcagc ttctcaagct ttaaccgctt tacaacaaga ctttcaattt
    42541 taagagaaag ttagggattc tatcaatttc atagaaattt tgatttagta aacgaagaga
    42601 caatcttata tgtcactcct catttaatac gccactactg gacaagcaaa atcattatta
    42661 cagcagttcc agtccttcaa ttaacagcaa cttacaatca agttgaggac gaacttgctg
    42721 aagcgacaac cgacctattt cttagtcata ttcgctaaaa aaatccccgc caaaatctca
    42781 aaaagtcccc gccaattccc cgaccaaaat ccgaaaaata ccgaaaaata tcgaaaaatt
    42841 atttttagaa tagtccccaa aagcctgaaa tagagccaaa aaactccacc tgattgggtg
    42901 gagttaaggg agattattat aaaaaagaaa agtttaggat tttattaaat aaagttagga
    42961 ggtctttatt taataactat atgatacaag acgaagctta aaactagctt aacttttctc
    43021 aaattatacc atttttaaaa aaatttctat cagcatcacc tcaccctaca aaaaagccac
    43081 ctgattaggt gacttcatta ggagattatg atgaaaaaag ttttaggatt tcattaaata
    43141 aagttaggag gtctttattt aatgactata tgatactaga ccagccttaa actttgctta
    43201 agaacaacca agttttatta tttttctctg tttatccaag tcattcctat acaatcatag
    43261 gtggataaga tttcaaagcc catagaacaa tagaagccca aggttctttc tgtctgttct
    43321 gttaccagct ggacttgata ggcatctttg taatcctcta aagcctcttt cattagggta
    43381 ctaccaatcc cttgacgttg atagattggt aaaacgatta aatcctgaac caatactgat
    43441 gagaatccat ctccaaccaa acgaatcaag cccaccacag catcgccatc aagcgccaca
    43501 taaatcgcta atgaatgaga taaggcctgc tccagcatct ctggttgatg ggtataattt
    43561 gtccaaccga cagcctgata gagatgcaaa acatcctcta gcttgacaaa ttcttgcttt
    43621 ttaatagtaa tcatctcaac acctcttaaa gctctctcaa gctcttgtac tgctgtccat
    43681 ttttatcaaa attttcagga cgcaaccatg tttccaaacg atctttgact ttgggccagt
    43741 ccttatcagt catagacaac caatccgtat ccctcgtacg ccccttataa accactgcct
    43801 gacggaaggt tccttcataa ataaagccca aacgttctgc agctcgtctt gatggcagat
    43861 taaaagcatc gcatttccac tcatagcgac gatagttaag ctcttcaaag acatagcgag
    43921 ccagtagata ctgggcttct gtccctatcc gtgtccccct gagttctgga gaaaaagtga
    43981 cagctcccac ttctattact cggttattct ggtcaatgcg catgagagaa aaagttccca
    44041 aaaccttacc agttgacctg tctatgattg cataataaaa acggtcctta cgagccaaca
    44101 tctgatttaa aaggctgacc agttcctcca tgtctgctac tggttcctga aagaggtagg
    44161 tccacatctc ctgaggagta tccgggccat aaacagctaa caaatcctcc gcatgttttt
    44221 ctaccgagag agcctctatc cgagcataac gtccttctaa gaaatcaata aaagtcagtt
    44281 cacctggtgt ataaccttcc attgactcac caatcatctg accatattcg tttactggta
    44341 taactttctc cttgtttcgc ttttcaaact ttatagttgc ccatagtata gcgta
//
LOCUS       DAVMAF010000008        43814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMAF010000008 DAVMAF010000000
VERSION     DAVMAF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.008"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            95..166
                     /locus_tag="VYO97_000420"
     tRNA            95..166
                     /locus_tag="VYO97_000420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Glu,seq:ttc)
     gene            172..245
                     /locus_tag="VYO97_000421"
     tRNA            172..245
                     /locus_tag="VYO97_000421"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Asn,seq:gtt)
     gene            complement(286..828)
                     /locus_tag="VYO97_000422"
     CDS             complement(286..828)
                     /locus_tag="VYO97_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1753282.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            1007..2569
                     /locus_tag="VYO97_000423"
                     /pseudo
     CDS             1007..2569
                     /locus_tag="VYO97_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(2599..5151)
                     /locus_tag="VYO97_000424"
     CDS             complement(2599..5151)
                     /locus_tag="VYO97_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1753283.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTKAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5213..6835)
                     /locus_tag="VYO97_000425"
     CDS             complement(5213..6835)
                     /locus_tag="VYO97_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1753284.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            7041..8066
                     /gene="trpS"
                     /locus_tag="VYO97_000426"
     CDS             7041..8066
                     /gene="trpS"
                     /locus_tag="VYO97_000426"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1753285.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            8218..9696
                     /gene="guaB"
                     /locus_tag="VYO97_000427"
     CDS             8218..9696
                     /gene="guaB"
                     /locus_tag="VYO97_000427"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1753286.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(9747..10844)
                     /gene="recF"
                     /locus_tag="VYO97_000428"
     CDS             complement(9747..10844)
                     /gene="recF"
                     /locus_tag="VYO97_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1753287.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(10847..11215)
                     /gene="yaaA"
                     /locus_tag="VYO97_000429"
     CDS             complement(10847..11215)
                     /gene="yaaA"
                     /locus_tag="VYO97_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1753288.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            11374..12624
                     /locus_tag="VYO97_000430"
     CDS             11374..12624
                     /locus_tag="VYO97_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1753289.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            12621..13904
                     /locus_tag="VYO97_000431"
     CDS             12621..13904
                     /locus_tag="VYO97_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1753290.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13946..14767
                     /locus_tag="VYO97_000432"
     CDS             13946..14767
                     /locus_tag="VYO97_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1753291.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            14778..15323
                     /gene="pgsA"
                     /locus_tag="VYO97_000433"
     CDS             14778..15323
                     /gene="pgsA"
                     /locus_tag="VYO97_000433"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1753292.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            15320..16147
                     /locus_tag="VYO97_000434"
     CDS             15320..16147
                     /locus_tag="VYO97_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1753293.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            16132..16971
                     /locus_tag="VYO97_000435"
     CDS             16132..16971
                     /locus_tag="VYO97_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1753294.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPVKIEEFKES
                     LNG"
     gene            16964..17758
                     /locus_tag="VYO97_000436"
     CDS             16964..17758
                     /locus_tag="VYO97_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1753295.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            17817..18635
                     /gene="mreC"
                     /locus_tag="VYO97_000437"
     CDS             17817..18635
                     /gene="mreC"
                     /locus_tag="VYO97_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1753296.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            18635..19129
                     /gene="mreD"
                     /locus_tag="VYO97_000438"
     CDS             18635..19129
                     /gene="mreD"
                     /locus_tag="VYO97_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1753297.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            19223..20401
                     /locus_tag="VYO97_000439"
     CDS             19223..20401
                     /locus_tag="VYO97_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1753298.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            20625..21404
                     /gene="rpsB"
                     /locus_tag="VYO97_000440"
     CDS             20625..21404
                     /gene="rpsB"
                     /locus_tag="VYO97_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1753299.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNK"
     gene            21470..22510
                     /locus_tag="VYO97_000441"
     CDS             21470..22510
                     /locus_tag="VYO97_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1753300.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(22631..23557)
                     /gene="cysK"
                     /locus_tag="VYO97_000442"
     CDS             complement(22631..23557)
                     /gene="cysK"
                     /locus_tag="VYO97_000442"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1753301.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVYAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEFEV"
     gene            complement(23842..24477)
                     /locus_tag="VYO97_000443"
     CDS             complement(23842..24477)
                     /locus_tag="VYO97_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1753302.1"
                     /translation="MEFRIIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            24533..25831
                     /locus_tag="VYO97_000444"
     CDS             24533..25831
                     /locus_tag="VYO97_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1753303.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            25828..26490
                     /locus_tag="VYO97_000445"
     CDS             25828..26490
                     /locus_tag="VYO97_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1753304.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNNDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            26570..27118
                     /gene="raiA"
                     /locus_tag="VYO97_000446"
     CDS             26570..27118
                     /gene="raiA"
                     /locus_tag="VYO97_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1753305.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            27254..29227
                     /locus_tag="VYO97_000447"
     CDS             27254..29227
                     /locus_tag="VYO97_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1753306.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNIQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            29224..29676
                     /gene="rplI"
                     /locus_tag="VYO97_000448"
     CDS             29224..29676
                     /gene="rplI"
                     /locus_tag="VYO97_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1753307.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            29720..31072
                     /gene="dnaB"
                     /locus_tag="VYO97_000449"
     CDS             29720..31072
                     /gene="dnaB"
                     /locus_tag="VYO97_000449"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1753308.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            31074..31340
                     /locus_tag="VYO97_000450"
     CDS             31074..31340
                     /locus_tag="VYO97_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753309.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            31429..32775
                     /gene="cbpD"
                     /locus_tag="VYO97_000451"
     CDS             31429..32775
                     /gene="cbpD"
                     /locus_tag="VYO97_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1753310.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      32858..32960
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33031..33321
                     /locus_tag="VYO97_000452"
     CDS             33031..33321
                     /locus_tag="VYO97_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1753311.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            33284..34042
                     /locus_tag="VYO97_000453"
     CDS             33284..34042
                     /locus_tag="VYO97_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753312.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            34080..35087
                     /locus_tag="VYO97_000454"
     CDS             34080..35087
                     /locus_tag="VYO97_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1753313.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            35087..35815
                     /locus_tag="VYO97_000455"
     CDS             35087..35815
                     /locus_tag="VYO97_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753314.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            35934..36392
                     /locus_tag="VYO97_000456"
     CDS             35934..36392
                     /locus_tag="VYO97_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1753315.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            36394..38826
                     /locus_tag="VYO97_000457"
     CDS             36394..38826
                     /locus_tag="VYO97_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1753316.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQAHSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDEESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            39147..39800
                     /gene="rr06"
                     /locus_tag="VYO97_000458"
     CDS             39147..39800
                     /gene="rr06"
                     /locus_tag="VYO97_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1753317.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            39797..41137
                     /gene="hk06"
                     /locus_tag="VYO97_000459"
     CDS             39797..41137
                     /gene="hk06"
                     /locus_tag="VYO97_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1753318.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKQLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVNVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            41232..41759
                     /locus_tag="VYO97_000460"
     CDS             41232..41759
                     /locus_tag="VYO97_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1753319.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            41838..>43814
                     /locus_tag="VYO97_000461"
     CDS             41838..>43814
                     /locus_tag="VYO97_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1753320.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVRRGN
                     NLTVTSSGDEVESHYQSILEKVRKSLEKDRHTQNVDLIKKLQDIKRTYLYNLKEKPEA
                     ELTSKTKKELDAAFEKFKKEPELTKKLAEAEKKVAEAEKKAKDQKEEDHRNYPTNTYK
                     TIELEIAEAEVGVAKAELELAQAQVQIPQDTEKINAAKAKVEAAKSNVKKLEKIKSDI
                     EKTYLYKLDNSTKKTPKPRVRRNSPEIKAKGRVKNYKEANIELSKYMTDLYKLDNSTK
                     ETPKSRVRRNSPQVGDSRELKETIDKAKETLSTYMVTRLTKLDPSVFWFADLLMDAKK
                     VVEEYKTKLEDASDKKSVEDLRKEAEGKIESLIVTHQNREKENQPAPQPGGQAGGSMV
                     VPPVTQTPPSTSQSPGQKATEAEKKKLQDLIRQFQEALNKLDDETKTVPDGAKLTGEA
                     GKAYNETRTYAKEVVDKSKKLLSQTAVTMDELAMQLTKLNDAMSKLKEAKAKLVPEVK
                     PQPENPEPKPQPEGEKPSVPDINQEKEKAKLAIATYMSKILDDIKKHHLKKEKHHQIV
                     ALIKDLDKLKKQALSEIDNVNTKVEIENTVHKVFADMDTVVTKFQKGLIQNTPQVPEA
                     PKSPEVPKVSDTPKAPDTPQVPEAPKSPEVPKVPE"
BASE COUNT        13948 a         7097 c         9434 g        13335 t
ORIGIN      
        1 tagaaaatta aaagatattt tagagaaaaa atctcaaaag tgattgacaa ttagcaagaa
       61 attgatataa tggttatatc tgctacagat agaaggtccg ttggtcaagg ggttaagaca
      121 ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatttt atccgccata
      181 gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgg
      241 cggagtatag ataaaaggga acacagctgt gttcctcttt ttgtatcaat ttgtatcacc
      301 aagcattttc ataaggaagt ctgttatttc ttgagaactt tctttttttc catgtgcaat
      361 ccaagtttgg cagacaccaa aaagtgcatg agttagatag atgctactat attctaattc
      421 ggtggtattt agattcagtt gcataaatcg cttttgtaaa tctgtactaa gcatgatatg
      481 aagtttattt cgtaagaaat tttggatttc tttagtccca ttttcagaaa gaagggcagc
      541 cagaagtggt tctgactcta gatattcaaa aacttctaaa atagcgtctc ttttgtgatg
      601 agcatgtttt tgaaaaatat attcaaatgt atggaatagc ttgctttgat agtgctcaat
      661 catatcatac ttatccttat agtgagtata gaagctggaa cgactaattc cggctttttc
      721 tactaatttg acagtagaaa ttttatcaaa tggctgttcc atcagtaatt gtaccatagc
      781 attttcaata gttcgctttg tttttaagcg tttgttactt tcttgcatat ttcctccttg
      841 taaacaaatt agactatatg tctaaaaata gattttttat cttgtaattt agatttttta
      901 atgtataatc tattatatca aaattttaga caatatgttt aaaaaaggag aaactaagtt
      961 taaagaatgg aaagcaattt aaaaaaaacc aacctttatt attgtcatga tcgggatttc
     1021 tcttattcca gatctgtaca atatcatatt tttgtcatca atgtgggatc catatgggca
     1081 attgtctgac ttacctgtgg cagttgtaaa taatgataaa gaggcttcct ataatggtaa
     1141 tactatggca ataggaaaag acatggtgtc caatttaaaa gaaaataaaa ccttggattt
     1201 tcattttgta gatgaagagg aaggaaagaa gggattggaa gatggcgatt actatatggt
     1261 agtgacttta ccaagtgatt tatctaaaaa aacaactaca ttatccaata ttcaatcgac
     1321 agcagcttat caatcattga caagtgagca acaaactggg ataagtgatt ctgtatctca
     1381 aaattcaact gatagtattc aatcggctca gtcaattgta gctttagtac aaggtttaca
     1441 gggaagttta gaaaacttac aaaatcaatc ttctaatctt tcgactttaa aaaatcaagc
     1501 taatcaagta ttacctatta cttctacttc tttgatagga ttgtcaagtg gattaacaga
     1561 gatacaagga gctgttacta gcaaattagt tcctgccagt cagttgattg catcaggtgt
     1621 aaacgcatat actacaggtg ttgataaagt ttctcagggc gcaagtcaac taagtgaaaa
     1681 aaatgccacc ttgacacaaa aatcttctag attgacagca ggagttgata aattacaatc
     1741 aggatctggg caattagcag acaaatccag tcagttactt tcaggtgctt cttcattaga
     1801 gaatggagct aataaattgg cagatggatc tgggaaacta gcagaaggtg gaacaaagtt
     1861 aacttctgga ttggaagatt tacagacagg acttgcttct ttaggacaag gactaggtaa
     1921 tgctagtgat caactcaaat cagtatcaac agaatctaaa aatgcagaga ttttgtcaaa
     1981 tccactcaat ctttcaaaaa gcagacaatg atcaagttct tgtaaatgga atcgcaatag
     2041 ctccttatat gatatcagtt gctctttttg ttgcagcaat atcaacaaat atgatatttt
     2101 cgaaattgcc ttcaggacgt catccagaga tccgttgggc ttggttgaaa tctcgagctg
     2161 aaataaatgg tattatagct gttttggcag gaattttggt atatggagga gttcatctta
     2221 ttggtttaac tgctaatcat gagatgagaa tatttattct catcatccta acaagtttag
     2281 tattcatgtc tatggtgacc actttaacaa cgtggaatag ccgtatagga gctttcttct
     2341 cacttatttt gcttttacta cagttagcat caagtgcagg tacttatcca cttgctttga
     2401 caaatgattt ctttaaatct attaatccct ggttaccaat gagctattca gtttcgggat
     2461 tacgacaaac aatctccatc aacaagtcat tttcctagct gtcatactag ctctatttac
     2521 tggtttaggt atactagcct atcaacataa gaaaatggaa gaagattaaa aaaatcgacc
     2581 gattaactgg tcgatttttt atgccttaga tgactttcgt ctgtgattat agattccaaa
     2641 tagtaagaga gaagtaaagg aacagattgc tccagtaata aaaccattgg gaatgaagga
     2701 aagtgtaata gttcctttcc ccttgggaat gtcaactttc ataaatccag tttgagcttg
     2761 tttaatttct attttcttac catcttggta ggcagaccaa cctttgtcat aaggaatggt
     2821 gaagaaaata gatgtatctt gttggacatc atatgtagca aaaaccttgt ttttagaagt
     2881 tgatactgtg acaggttgtt ctttaatttt ttgaattgcc ttggtgaaag ttttggtatc
     2941 taaacgatag aaggtaggag attcaaatga tacttgtgaa tttccaggga aactaacatt
     3001 gatattgaaa gtttttttct ctttagtata tcctagatta aagaaggaga agacattatc
     3061 agttgtaaaa gtcttttttt caccatttac aaggatgtca accttctttt gtttatcgtt
     3121 agaaaagtga aggtttatga aagagagata aacttggctg ttttctggaa cttcaatttg
     3181 atactggatt gctgcatctt catttgaaga acttgtgaca ctaatcaaat cattagtatt
     3241 ttctattttt tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc
     3301 aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt
     3361 gtaaacagat tgactagcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag
     3421 attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt
     3481 gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag
     3541 attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg
     3601 aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg
     3661 agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat
     3721 agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc
     3781 gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat
     3841 ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac
     3901 aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt
     3961 tagatagatg gtagctaaaa atcctgttac tacaagaaaa agcgaaacta aaaaattcca
     4021 gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga
     4081 gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa
     4141 gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag
     4201 tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa
     4261 tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa
     4321 ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt
     4381 caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa tcaaactagc
     4441 cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa
     4501 gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa
     4561 ataataattt tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc
     4621 agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca
     4681 ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc
     4741 tagttttaaa gtctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag
     4801 accaatcaat ccaaatttta agagagtggt cagatagaca gcatctggca tgttcgttag
     4861 atcaaaaaag taaactagag gcgcgagaaa actacccaag taataactag atagggcata
     4921 gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ctactatttc catgtaggat
     4981 atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga
     5041 gttgtcgcta ttccagtaga taccttgaga tagatatact ccagacataa tcactacagg
     5101 aatgatgaga gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg
     5161 tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt
     5221 tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt
     5281 catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt
     5341 gaataaattc gtggtcatgg ctagcaaaga tgattgattc tttaaagttt ttcaatccat
     5401 cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga
     5461 catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca
     5521 agacatttac aggtttgtta acttcatctc cagagaagag catacggccg aggaagccac
     5581 gtaggaaagt attgtcatct tcttctttac ttgcgaattg acgcaaccag tcaagaattg
     5641 attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga ctagttgtaa
     5701 ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag
     5761 tcgtttgaat atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac
     5821 taatattatc caagatagtt tcaccatcaa tctttacagt tagattttct actgtcaaga
     5881 gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg
     5941 gtacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg
     6001 acttagaagc attggcagag aaacgagcaa cgaattcttg caattgttta attttttctt
     6061 ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga
     6121 agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac
     6181 aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa
     6241 tcaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca
     6301 agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac
     6361 cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa
     6421 gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga
     6481 gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt
     6541 tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt
     6601 catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag
     6661 agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc
     6721 cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat
     6781 catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatattt
     6841 ttctcctata tgtgtaatat atttattcta ctagaaaata cagaaatatt caaattttta
     6901 tttgtcaatt ttgtgtaaat tatatttaca gtatccttta cacaaatctg taaaaagcaa
     6961 ggctgattta ttttgataaa ttacggttat ttcattaaaa aaatgctata attgaaagga
     7021 ctatatcgaa ggagaacaaa atgactaaac ccattatttt aacaggagac cgtccaacag
     7081 gaaaattgca tattggacat tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag
     7141 aggataagta tgatatgttt gtgttcttgg ctgaccaaca agccttgaca gatcatgcca
     7201 aagatcctca aaccattgta gagtctatcg gaaatgtggc tttggattat cttgcagttg
     7261 gattggatcc aaataagtca actattttta ttcaaagcca gattccagag ttggctgagt
     7321 tgtctatgta ttatatgaat ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca
     7381 agacagagat ttctcagaaa ggatttggag aaagcattcc gacaggattc ttggtctatc
     7441 caatcgctca agcagctgat atcacagctt tcaaggctaa ttatgttcct gttgggacag
     7501 atcagaaacc aatgattgag caaactcgtg aaattgttcg ttcttttaac aatgcatata
     7561 actgtgatgt cttggtagag ccggaaggta tttatccaga aaatgagaga gcagggcgtt
     7621 tgcctggttt agatggaaat gctaaaatgt ctaaatcact aaataatggt atttatttag
     7681 ctgatgatgc ggatactttg cgtaaaaaag taatgagtat gtatacagat ccagatcata
     7741 tccgcgttga ggatccaggt aagattgagg gaaatatggt tttccattat ctagatgttt
     7801 ttggtcgtcc agaagatgct caagaaattg ctgatatgaa agaacgttat caacgaggtg
     7861 gtcttggtga tgtgaagacc aagcgttatc tacttgaaat attagaacgt gaactgggtc
     7921 ctattcgtga gcgccgtatt gaatttgcta aggatatggg agaagtttat aatatgattc
     7981 aaaaaggtag tgaaagagcg cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg
     8041 caatgggact tcattacttt aactaagttt attttacaag aaactatcat ttctatctcg
     8101 aagaaaagat agttttttat ttacccctgt aacatttgac aaatttttat agaatggtat
     8161 gatagataca atataaaaag agtcaagctc aaaaagaaag aaaagaggaa acttcgaatg
     8221 tctaattggg acactaaatt tttgaaaaaa ggttttacct ttgatgatgt attgcttatt
     8281 ccagctgaaa gtcatgtgtt gcctaacgat gcagatttaa caactaaatt ggcagataat
     8341 ttgactttaa atatcccaat tattaccgct gccatggaca cagttacaga gagtcaaatg
     8401 gccattgcta ttgctcgtgc aggtggtctc ggagttatcc ataaaaacat gtcaattgct
     8461 caacaagcag acgaggttcg taaggtaaaa cgttctgaaa atggagttat tattgatccg
     8521 ttcttcttga cgcctgaaca tacaattgct gaagcagatg agcttatggg tcgttaccgc
     8581 atcagtggtg ttccagttgt tgaaacactt gaaaatcgta aattggttgg tattttgaca
     8641 aaccgagatc ttcgttttat ttcagattat aatcaaccaa tttcaaacca tatgactagt
     8701 gaaaatcttg ttactgctcc tgtgggtacg gatcttgcaa cggctgagag tattcttcaa
     8761 gagcatcgta ttgaaaaact tccgttggtc gatgaagaag gcagtctttc tggtttgatc
     8821 actatcaaag atattgaaaa agttattgag tttccaaatg cggctaaaga tgaatttggt
     8881 cgtcttctag ttgcaggtgc agtaggtgtt acttcagata catttgaacg tgcagaggct
     8941 ctttttgagg caggagcgga tgcgattgtt attgatactg cacatggtca ttctgcaggt
     9001 gtcttgcgta aaattgccga gattcgtgct catttcccag atcggacttt gattgctgga
     9061 aatattgcta ctgctgaagg tgcacgtgcc ctttatgaag cgggtgtaga cgttgttaag
     9121 gttggtattg gaccaggttc tatctgtact actcgtgtga ttgctggtgt tggtgttccg
     9181 caagtaacag ctatctacga tgctgcagct gttgcgcgcg aatatggtaa aacgattatt
     9241 gctgacggtg ggatcaagta ttctggagat attgtaaaag cacttgctgc aggtggaaat
     9301 gctgttatgc ttggatctat gtttgctgga actgatgaag ctccaggcga aactgaaatc
     9361 ttccaaggac gtaaattcaa gacttaccgt ggtatgggat caattgctgc tatgaagaaa
     9421 ggttcaagcg accgttattt ccaaggttct gtcaatgaag caaacaagct cgttccagaa
     9481 ggaattgaag gtcgtgttgc ttataaagga gcggcagctg atattgtttt ccaaatgatt
     9541 ggtggtattc gctctggtat gggttactgt ggtgcagcta accttaaaga actacacgat
     9601 aatgctcaat ttattgaaat gtctggtgct ggtttgaaag aaagccatcc tcatgatgtg
     9661 caaattacta atgaggcacc aaattattct atgtaaaaaa caatgaaaag aactccagtg
     9721 aaaacaggag ttcttttaca atgttgtcaa tttccattta cagaagcttt accatcctga
     9781 atagtgaaga tacttagatt ttctggcaga ttttgaagat ggtctaagct tgttgttgtg
     9841 ataaaggttt ggattgattg agaaatcgtt tctaataatt ttaactgtct agtgttgtca
     9901 agttcactca tcacatcgtc aagcagtaat ataggagatt ctgtggtaat gctttccatt
     9961 aattcgattt ctgctaattt tatcgagagg acgagactac gatgttgacc ttggcttccg
    10021 aaactagcat ccatcccatt tatataaaaa gaaatgtcat ctcgatgagg accgacacca
    10081 gtattctttt taaataaatc tctggatcta cttttttcta aagcaatttt gaaagattcg
    10141 gataagtttt gtttgtcagt tatattgaca gaagattgat aggatattga caactcttcg
    10201 atctgattag agagttcaaa atgtttctta cgcccaaatg attctagttt ttttatgaaa
    10261 tctaagcggt gattcattac acgacatcca taatcaacta gctgatcatc taacacagaa
    10321 aggaatgttt catctatttt ttgagctgat tttaggtaag tgtttctttg ctttaggatg
    10381 tggttataat tggttaagtc agataaatag attggcttaa tttgcccaag ttccatatca
    10441 atgaattttc gtcgaatcga aggtgctcct ttaattagtt gtaaatcttc aggagcaaat
    10501 aagacaacat tcatgtgtcc tacataatct gaaaggcgtg cctgttttaa gtgattaact
    10561 tttgttacac ggcctttttg tgttagttcg atttctaggg gaatggatcc agtttttttc
    10621 tgaacgagac ctgaaagatg aagttgttcc tcatcaaaat gaatgagatt tttatctgtt
    10681 cgagttcgat gactacgcgt taaggctaaa aaatagatag cctctaacat gtttgttttt
    10741 ccttgtgcat tacgtcccaa aaagacattc aatttaggat taaagtctat tttcgtttct
    10801 ttgtaattac gaaaagtctt gagagataga tgttgtagcc acatgattat ctaccaggga
    10861 atcgtggagc ttgttggctt ttaggtgatg aagcaggttt cgatttgtct tttttaactc
    10921 ccttattcat ctttttgaca agtttagcga tccgttcttt ttcaacttta tcagcttggt
    10981 attcatcttg ttcttcagaa gtaggttgtg tcaacaagat gtcaatgttc atgtcaggga
    11041 tgtcaacttt atcgccaata cgaagttttt ttccacgacg actctctaat tccctattaa
    11101 agtaaacaga atgttcagag agaaatgatt taatagctcc tccgctatgt gtaattccaa
    11161 gttctttgag tagtgcttgg agggtaataa attcttcaaa taatttgtat tccataattc
    11221 acctcaatct ttggtattat accatatttt cctaaaaata gtgagagaga cagcgagaaa
    11281 tgtaagcgat ttttatttca aaagtgttac agggaacaag aaaatttcag gttttcgtga
    11341 tataatagaa gtctgtatat aaggaggtaa atcatggagt tagtgcatgg aatttcaaca
    11401 cattttatcc aatcaaaaaa gtttaaaaca aacaaaatta ccgtgcgttt taccgctcca
    11461 ttatcccttg atacgattgc aggtcacatg ttgagtgcaa gtatgctaga gactgctaat
    11521 cagatgtacc ccacttctca agatttgagg agacacttgg ccagtctata cggtacagat
    11581 atgtcaacca attgtttcag aagagggcaa agccacatta tagaattgac atttacctat
    11641 gttcgtgatg agtttttaag taggaaaaac gtgctaacct ctcagatttt ggaacttgta
    11701 aaagaaactc ttttttcacc cgcagtagtt gataatgggt ttgatccggc cttatttgaa
    11761 attgagaaaa aacaattgct agcaagttta gcagctgata tggatgattc tttttatttt
    11821 gcacataaag aattggataa attgtttttt catgatgaac gtcttcaatt ggaatatagt
    11881 gatttacgaa atcgtatttt agctgaaact ccacaaagtt cttattcttg tttccaagaa
    11941 tttttagcca atgatcgaat agatttcttt ttcctaggtg attttaatga ggttgaaatt
    12001 caaaatgtat tagaatcatt tggctttaaa ggtcgaaaag gagatgtgaa ggttcagtat
    12061 tgtcaacctt attctaatat ccttcaggaa ggtatggttc ggaaaaatgt gggacaatcc
    12121 attttggaat taggttatca ttaccgttct aaatatggtg atgagcaaca tttacccatg
    12181 attgtaatga atggtttact tggtggattt gctcactcta agctctttac aaatgtccgt
    12241 gaaaatgctg gattagctta taccatttca agtgagcttg atttatttag tggattcttg
    12301 aggatgtatg ctggtatcaa tcgagaaaat cgtaaccagg ctcgtaaaat gatgaataat
    12361 caactgcttg atttaaaaaa aggttatttt acagagtttg agttaaatca gaccaaggaa
    12421 atgattcgtt ggtcgttgtt actttctcaa gataatcaat cttcattgat tgaacgtgct
    12481 tatcaaaatg ccttatttgg aaaatcttca gcagacttta aaagttggat tgcaaagctt
    12541 gaacaaattg acaaagatgc tatttgtaga gtagctaata atgtgaaact acaagcgatt
    12601 tactttatgg aaggaataga atgacaaagg ttgtttttga agaaaaatac tatccagctg
    12661 taaaagaaaa ggtttatcga actcgtttgg ccaacggatt gacagttgct cttttgccta
    12721 aaaaggaatt taaagaggtt tacgggagtg tcactgtaca gtttggttcg gtagatacgt
    12781 ttgtcacaga agttgacgga tatgtaaaac aatatcctgg aggaattgct cattttcttg
    12841 aacataaatt atttgagaga gaagattcta gtgatttgat gtcggctttt acgagtctag
    12901 gtgcagatag taatgccttt acaagcttta caaaaacaaa ctatcttttt tcagcaacgg
    12961 attatttttt agaaaattta gatttacttg atgaattggt aacatcagca cactttactg
    13021 aagcttccat tctgacagag caggatatta ttcagcaaga acgagaaatg taccaagatg
    13081 atccagattc gtgtttattc ttttcaactt tagcgaattt gtatcctggt acacctttag
    13141 caactgatat agttggaagt gaggagtcca tttcccaaat caatctaact aatttgcaag
    13201 aaaattttac aaagttttac aaacctgtaa acatgtctct gtttttagtt ggtaattttg
    13261 atgtggagcg agtacaggac tattttgaaa gcaaagaact gaaagattca gattttcagg
    13321 aagtagcaag agaaaagttg tttttacagc ctgtaaagcc aacagatagt atgagaatgg
    13381 aagtatcttc tcccaaacta gcgattggag ttagaggtaa gcgagaagtt tctgaagcgg
    13441 attgctatcg acatcatatt ttattaaaat tattgtttgc aatgatgttt ggttggactt
    13501 cggatcgttt tcaaaaatgt tatgaatcag gtaaaattga tgcgtcctta tctctggaag
    13561 ttgaaataac aagtcgcttt cattttgtca tgttgacaat ggatacgaaa gagccagttg
    13621 ctttgtctca tcaatttagg aaggctattc gtaattttac aaaggattta gatattacag
    13681 aggaacattt agatattatc aaaagagaga tgtttggcga atttttcagt agcatgaact
    13741 ctcttgaatt tattgcaacg caatatgatg cttttgaaaa tggtgagaca atttttgatt
    13801 tgccgaaaat tttacaggaa attactttag aggatgtcct tgatgctgga catcatttaa
    13861 tagatgatgg tgacatagtt gattttacaa tatttccatc gtagtaacct atcataatag
    13921 acactagaaa gaagggatga caagtatgag aaaaaaaaca attggagagg ttttacgatt
    13981 agctagaatc aatcagggat tgagtttaga tgaattgcag aaaaagacag aaatccagtt
    14041 agatatgttg gaagcaatgg aagcagacga tttcgatcaa cttccaagtc ctttttacac
    14101 gcgttctttc ttgaaaaaat atgcatgggc tgttgagtta gatgaccaaa ttgttttgga
    14161 tgcttatgat tctgggagta tgattactta tgaggaagta gatgttgatg aagatgagtt
    14221 gacaggtcgt agacgttcaa gtaagaaaaa gaagaaaaaa acatcatttt tacctttatt
    14281 ttattttatc ctttttgctt tatcgatttt aatttttgtg acttattatg tttggaacta
    14341 tattcaaact caaccagagg agccttctct ttctaattac agtgtggttc aatcaacaag
    14401 ttcaactagc tctgttcccc actcctcaag tagtagttct tctagtatag aatcagctat
    14461 aagtgtatca ggcgaaggaa atcatgtaga aatcgcttat aagacaagta aggaaacagt
    14521 taaattgcaa ttggcagttt cagatgttac aagttgggtc agtgtttcag aaagcgaact
    14581 tgagggcggt gtaaccttat cgccaaagaa gaaaagtgca gaagcaacag ttgcaactaa
    14641 aagtcctgta acaattacgt taggtgttgt aaaaggtgtt gatttgacag tagataatca
    14701 gactgttgat ttatcgaaat taacagctca gactggacaa atcactgtaa cctttactaa
    14761 aaattaagga aaaacgaatg aaaaaagaac aaattcccaa tctcttaaca ataggtcgaa
    14821 ttctctttat acctattttt atctttattt taacgatagg aaattcgata gagagtcata
    14881 tagttgcagc tattatcttt gctgttgcca gtattaccga ctatttagat ggatatttag
    14941 ctcgtaaatg gaatgtggtc agtaattttg gtaaatttgc agatcctatg gcggataagt
    15001 tactagttat gtcggctttt attatgttga ttgagttagg tatggctccg gcttggattg
    15061 ttgcagtgat tatctgtcgt gagttagctg tgacaggttt aaggctttta ttggttgaaa
    15121 ctggtggaac aattttagca gcagcaatgc ctggaaaaat taaaactttt agtcagatgt
    15181 ttgccattat tttcttgcta ttacattgga ctttgcttgg tcaagttcta ctttatgtag
    15241 ccttattttt cactatctac tctggctatg actatttcaa gggtagtgcc tatgtattta
    15301 aagggacatt tggttcgaaa tgaaatcaat aattgatgta aaaaatcttt cttttcgcta
    15361 taaagaaaat cagaactact acgatgtgaa ggatattacg tttcacgtga aacgtggaga
    15421 atggctttcg attgtagggc ataatggtag tggtaaatca acgacggttc gattaattga
    15481 tggcttactg gaagcagaat ccggagagat tgtaattgat ggccaacgtt tgactgagga
    15541 aaatgtttgg aatatacgtc gtcaaatcgg tatggttttt caaaatccag acaatcaatt
    15601 tgttggagcg actgttgaag atgatgttgc ttttggtttg gaaaatcagg gactttctcg
    15661 tcaagaaatg aaaaagagag tggaagaagc tctggcttta gttggcatgt tggactttaa
    15721 aaagagagag ccagcgcgtc tatcaggtgg ccaaaagcaa cgtgtggcca ttgcaggtgt
    15781 tgtagcccta agaccagcta ttttaatctt agatgaagca acgagtatgt tggatcctga
    15841 ggggcgtaga gaacttattg ggacagtaaa aggaattcga aaagactatg atatgacggt
    15901 catttctatt acccatgatt tggaagaagt cgccatgagt gatcgcgtat tggtcatgaa
    15961 aaaaggggaa attgaatcaa ctagtagtcc aagggagctt ttctctcgaa atgatttaga
    16021 tcaaattgga ttagacgatc cttttgctaa tcaattaaaa aaatctttga gccagaatgg
    16081 ctatgattta cctgaaaatt atttgacaga aagtgagcta gaggataagc tatgggaatt
    16141 gctctagaaa atgtgaattt tatatatcaa gaaggtactc ccttagcttc agcagctttg
    16201 tcggatgttt ctttgacgat tgaagatggc tcttatacag ctttaattgg gcacacaggt
    16261 agtggtaaat caactatttt acaactctta aatggtttat tggtgccaag tcaagggagt
    16321 gtgagggttt ttgatacctt aatcacctcg acttctaaaa ataaagatat tcgtcaaatt
    16381 agaaaacagg ttggcttggt atttcagttt gctgaaaatc agatttttga agaaacggtt
    16441 ttgaaggacg ttgcttttgg accgcaaaat tttggagttt ctgaagaaga tgctgtgaag
    16501 actgcgcgtg agaaactggc tctggttgga attgatgaat cactttttga tcgtagtccg
    16561 tttgagctgt cagggggaca aatgagacgt gttgccattg caggcatact tgccatggag
    16621 ccagctatat tagtcttaga tgagccaaca gctggtctag atcctctagg gagaaaagag
    16681 ttgatgaccc tgttcaaaaa actccaccag tcagggatga ccatcgtctt ggtaacgcat
    16741 ttgatggatg atgttgctga atatgcgaat caagtctatg taatggaaaa gggacgttta
    16801 gtaaaggggg gcaaaccaag tgatgtcttt caagacgttg tttttatgga agaagttcag
    16861 ttgggagtac ctaaaattac ggccttttgt aaacgattgg ctgatagagg cgtgtcattt
    16921 aaacgattac cggttaagat agaggagttc aaggagtcgc taaatggata gtatgatttt
    16981 ggggcgttat atcccagggg attcgattgt tcaccgattg gatccacgta gcaaattgct
    17041 tgctatgatg ctactgattt tgattgtgtt ttgggctaat aaccccttga cgaatctgat
    17101 tctttttata gcgacaggga tatttattgc cttgtcagga gtatctcttt cattttttat
    17161 tcagggcttg aagtctatgt ttttcttgat tgccttcaca actatttttc aactattttt
    17221 catttctaat gggaatgttt tatttgagtt ttcgtttgtg agaatcacgg attatgcttt
    17281 gcaacaagct gggattattt tttgtcgctt tgtattgatt attttctttt caactttgtt
    17341 aaccttaaca accatgcctt taagtttggc atcagctgtc gaagctttat tagcaccttt
    17401 aaagcgtgtg aaagttccag ttcatgaaat tggattgatg ctgtctatga gtttgcgttt
    17461 tgtcccaacc ttgatggatg atacgactcg gattatgaat gcacagaaag ctcgtggagt
    17521 ggattttgga gaaggaagca tcgttcaaaa agtaaaggcg atgattccca ttttgattcc
    17581 tctttttgcg acaagtttaa aacgtgcaga ttccttggct atcgctatgg aagcgcgtgg
    17641 ctatcagggt ggaaaaggca gaagtcaata ccgacaattg aaatggactc taaaggatac
    17701 gctgaccatt cttgttattc tcgtacttgg ttgttgttta tttttcttaa aatcttagta
    17761 gctttcagga attgataaaa agttactgta acagtttttg atataaaggt agggatatga
    17821 accgttttaa aaaatcaaaa tatgtcatta ttgtttttgt cactgttctg cttgtgtcag
    17881 ctctcttagc gacgacttat tcaagtacaa ttgtgacaaa attaggagat ggaatctcat
    17941 tggttgatag agttgtacaa aaaccttttc agtggtttga ttctgtcaaa tcagatttgg
    18001 ctcatttgac acgaacatat aatgaaaatg aaagtttgaa gaaacagctt taccaattag
    18061 aagttaaatc aaatgaggtg gaaagtttaa agacagaaaa tgaacaactg cgccaattgc
    18121 ttgatatgaa gtctaaattg caagccacaa agactttagc agcagatgtt attatgcgtt
    18181 ctccggtatc ttggaagcag gagttgacct tagatgcagg tagatcaaaa ggtgcttctg
    18241 agaacatgtt agctattgca aatggtggct tgattgggag tgtttcaaaa gtagaggaga
    18301 actctactat agtcaacctt ctgacaaata cggaaaatgc tgataagatt tctgttaaaa
    18361 ttcaacatgg ctctactaca atttatggaa ttattattgg ctatgacaag gaaaatgacg
    18421 ttcttaaaat tagccaatta aatagtaata gcgatattag tgcgggagat aaggtgacta
    18481 ctggtggatt aggaaacttt aacgttgctg atattcctgt tggtgaagtg gttgccacaa
    18541 cgcatagtac agactatttg acacgagaag taactgttaa attgagtgca gatactcata
    18601 atgtagatgt gatagaatta gtggggaatt cataatgaga cagttgaagc gagttggagt
    18661 atttttattg cttcctttct ttgttctaat tgacgcccat attagccagc ttctgggctc
    18721 atttttcccc catgtacatt tggctagtca ttttcttttt ctatttctct tatttgagac
    18781 gatagaagta tcagagtatc tctacctagt ctattgtttt gttataggct tggtttatga
    18841 tgtttacttt ttccatctaa tagggattac aactctctta tttatcttat tgggagcctt
    18901 ccttcataaa ttgaatagtg ttattttgtt gaatcgttgg acaagaatgc tagctatgat
    18961 tgtgctgaca ttcctgtttg aaatgggtag ttatcttttg gcttttatgg tagggttgac
    19021 agtagatagc atgtcgattt ttatagtcta tagcttggta ccgacgatga ttttaaattt
    19081 tttatggatt actgtttttc aatttatttt tgaaaaatat tatctataag aacgacatat
    19141 aaatgtaaca aaggcgtaat atttattagg cctttttttg gtatactagt attgtcttta
    19201 aaagaaggag tatctacgta atatgaagaa aaaaatctta gcgtcacttt tattaagtac
    19261 agtaatggtt tctcaagtag ctgttttaac aactgcgcat gcagaaacga ctgatgacaa
    19321 aattgctgct caagataata aaattagtaa cttaacagca caacaacaag aagcccaaaa
    19381 acaagttgac caaattcagg agcaagtatc agctattcaa gctgagcagt ctaacttgca
    19441 agctgaaaat gatagattac aagcagaatc taagaaactc gagggtgaga ttacagaact
    19501 ttctaaaaac attgtttctc gtaaccaatc gttggaaaaa caagctcgta gtgctcaaac
    19561 aaatggagcc gtaactagct atatcaatac cattgtaaac tcaaaatcaa ttacagaagc
    19621 tatttcacgt gttgctgcaa tgagtgaaat cgtatctgca aacaacaaaa tgttagaaca
    19681 acaaaaggca gataaaaaag ctatttctga aaaacaagta gcaaataatg atgctatcaa
    19741 tactgtaatt gctaatcaac aaaaattggc tgatgatgct caagcattga ctacgaaaca
    19801 ggcagaacta aaagctgctg aattaagtct tgctgctgag aaagcgacag ctgaagggga
    19861 aaaagcaagt ctattagagc aaaaagcagc agctgaggca gaggctcgtg cagctgcggt
    19921 agcagaagca gcttataaag aaaaacgagc tagccaacaa caatcagtac ttgcttcagc
    19981 aaacactaac ttaacagctc aagtgcaagc agtatctgaa tctgcagcag cacctgtccg
    20041 tgcaaaagtt cgtccaacat acagtacaaa cgcttcaagt tatccaattg gagaatgtac
    20101 atggggagta aaaacattgg caccttgggc tggagactac tggggtaatg gagcacagtg
    20161 ggctacaagt gcagcagcag caggtttccg tacaggttca acacctcaag ttggagcaat
    20221 tgcatgttgg aatgatggtg gatatggtca cgtagcggtt gttacagctg ttgaatcaac
    20281 aacacgtatc caagtatcag aatcaaatta tgcaggtaat cgtacaattg gaaatcaccg
    20341 tggatggttc aatccaacaa caacttctga aggttttgtt acatatattt atgcagatta
    20401 atttacagag ggactcgaat agagccctct tttcaggttt taccgtgaca atccctatta
    20461 aaaattatat caaaatagct tgaaaatatt ggaaaagtat ggtagaatga aaattgtcgt
    20521 gtgaacgata atactcattc ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg
    20581 agttgaagtc aagaagagga aaaaaacaaa aaggagaaat actcatggca gtaatttcaa
    20641 tgaaacaact tcttgaggct ggtgtacact ttggtcacca aactcgtcgc tggaatccta
    20701 agatggctaa gtacatcttt actgaacgta acggaatcca cgttatcgac ttgcaacaaa
    20761 ctgtaaaata cgctgaccaa gcatacgact tcatgcgtga tgcagcagct aacgatgcag
    20821 ttgtattgtt cgttggtact aagaaacaag cagctgatgc agttgctgaa gaagcagtac
    20881 gttcaggtca atacttcatc aaccaccgtt ggttgggtgg aactcttaca aactggggaa
    20941 caatccaaaa acgtatcgct cgtttgaaag aaattaaacg tatggaagaa gatggaactt
    21001 tcgaagttct tcctaagaaa gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa
    21061 aattcttggg cggtatcgaa gatatgcctc gtatcccaga tgtgatgtac gtagttgacc
    21121 cacataaaga gcaaatcgct gtcaaagaag ctaaaaaatt gggaatccca gttgtagcga
    21181 tggttgacac caatactgat ccagatgata tcgatgtaat catcccagct aacgatgacg
    21241 ctatccgtgc tgttaaattg atcacagcta aattggctga cgctattatc gaaggacgtc
    21301 aaggtgagga tgcagtagca gttgaagcag aatttgcagc ttcagaaact caagcagatt
    21361 caattgaaga aatcgttgaa gttgtagaag gtgacaacaa atagtaatta cctaggaggg
    21421 cggggcttag cccggctctc ctattttcaa aaaatatagg agaattaaaa tggcagaaat
    21481 tacagctaaa cttgtaaaag agttgcgtga aaaatctggt gccggtgtta tggacgctaa
    21541 aaaagcgctt gtagaaacag acggtgacat cgaaaaagcg attgaattgc ttcgtgaaaa
    21601 aggtatggct aaggcagcta agaaagctga ccgtgttgct gcagaaggtt tgactggtgt
    21661 ttatgttaac ggtaatgttg cagcagttat tgaagtaaac gctgaaactg acttcgttgc
    21721 aaaaaacgct caattcgttg aattggtaaa tactacagct aaagtcattg ctgaaggaaa
    21781 acctgctaac aatgaagaag ctcttgcttt gataatgcct tcaggtgaaa ctcttgaagc
    21841 tgcatacgta tctgcaacag caactatcgg agagaaaatc tcattccgtc gctttgcatt
    21901 gattgaaaaa acagacgcac aacactttgg agcataccaa cataacggtg gacgtatcgg
    21961 tgttatttca gttgttgaag gtggagacga agcacttgct aaacaattgt caatgcacat
    22021 cgcagcgatg aaaccaacag ttctttctta caaagaattg gatgagcaat tcgttaaaga
    22081 tgagttggca caattgaatc acgttatcga ccaagacaac gaaagccgtg caatggttaa
    22141 taaaccagct cttccacact tgaagtatgg atcaaaagct caattaactg atgatgttat
    22201 tgctcaagct gaagctgaca tcaaagctga attggctgca gaaggcaaac cagaaaaaat
    22261 ctgggacaaa attattccag gtaaaatgga tcgcttcatg cttgataaca ctaaagttga
    22321 ccaagcttac acacttcttg cacaagttta catcatggat gacagcaaga cagttgaagc
    22381 ataccttgaa tcagttaacg cttcggtagt tgagtttgct cgctttgaag ttggtgaagg
    22441 tatcgagaaa gctgcaaacg acttcgaagc tgaagttgca gctacaatgg cagcagcctt
    22501 gaataactaa taatagaaag gaagcaaatt gcttcctttt ttgtatgcag tcgatcctta
    22561 tagggagagc ttcctatttt caaggtaaaa ataaaaagcc ctataataag ggccaattgt
    22621 atttaggaga ctaaacttca aattcataga gtgctgtaga gagataacgt tcaccgttat
    22681 ctggtgctag agcaaggact tttttacctg tacctaattt tttggcaacc tcgatggctc
    22741 cgtagatagc tgcagctgag gaaatcccta caaggaagcc ttcttttcca ccaatttcac
    22801 gtccgagtgc aagagcatca tctgatgtta cacgaacgat accatcatag gctttagtat
    22861 caagtgtatc aggaataaat ccagctgaga taccttgaat tttgtgagga ccaggttttt
    22921 caccagatag aatagcagat tcatcagctt ccactgcata aacttgaatg ttagaatttt
    22981 ctgatttgag tgcatgagaa acaccagaaa tcgttccacc ggtacctact ccagcaacaa
    23041 aggcatctaa tccatcttta ccgaaagcag ctagtatctc agctcctgtt gttctttcgt
    23101 gtacttctgg attagctgga ttgtcaaatt gaagaggaag gaaaccatca cgttcagcag
    23161 cgatttcttg agccttagca atagcacctt tcattccctc gctaccagga gttaggacga
    23221 gttcagcacc ataagcttgg ataattttac gtcgttctac actcatagtt tcaggcataa
    23281 cgatgacgac tttataccct ttagcagcac ctacccatga aagtccaata ccagtgtttc
    23341 cacttgttgc ttcaacaata gtagaaccag gtttcagaat accatcttgt tcagcttttt
    23401 caatcatgct aagggcaata cggtctttta cagatgaacc aggattaaat gcttcaagct
    23461 ttatatagac gtctgcagca ccttctggca cgatgttgtt aagtttaaca atcggtgttt
    23521 gaccgattaa ttcagtaatg ttgttataaa tagacatatg aatacctctt tgtataagat
    23581 atctttagta taacgctatc tataccattt gtaaaatata gaaatcctat cgaagcgata
    23641 ggatttctat atatcataga tcaaagtcat taattttcaa gtgattgcgt tcagctaatt
    23701 ctagtaaata ggtgtgaagg aaaacgatat caggtttcgt ttgaattgct tcattttcta
    23761 gtatattttg gtacgcatct ttttattttg ctcgctaaag tagttgtgag aatgtcaacg
    23821 aaacgctgta ttattcattg tttacactaa gtttacagga acttcaacct ctcgtaaacc
    23881 ttggtcagtt aaagtgactt ttccattaaa aaactccaca agtgcagctt taatagtttc
    23941 tttttcttcc ttatcaacat aaatcatcgt atcgacttga tctgtaaagt ttgtatccag
    24001 ctccatgaga tcatgttctt taaggaagtt actgtactct tggtactgag cataagacat
    24061 ttgaatagca atgccagcct gttcttttat ttcaataata ccaatttctt tgacagctaa
    24121 ggcgacactg ccggcgtaag cacgaattag tcctccagcg cctagtttaa taccaccaaa
    24181 gtagcgtgtc acgaccacac agacattggt gagattgtga ttttctagta ccccaagcat
    24241 gggaacacca gcagtaccac taggctcacc atcatcactt gtacgtttaa tttcactacg
    24301 ttctccaata ataaaggcag agcagttatg tgtcgctttg tagtgttctt ttttgatggc
    24361 agtaatgaag tcacgagcct cttcttcgct ataaacacgc ttggcatggc agataaagcg
    24421 agattttttg atttcttctt ggacttggcc gtcctcttta attatcctaa attccatgat
    24481 ttaattgtac taaaaaataa tctaaagtgc tagtttttac gaataaagaa gtatgaaagt
    24541 aaatttagat tatctcggtc gtttatttac tgagaatgaa ttaacagaag aagaacgtca
    24601 gttggcggag aaacttccag caatgagaaa ggagaagggg aaacttttct gtcaacgttg
    24661 taatagtact attctagaag aatggtattt gcccatcggt gcttactatt gtcgagagtg
    24721 cttgctgatg aagcgagtca gaagtgatca aactttatac tattttccgc aggaggattt
    24781 tccgaagcaa gatgttctca aatggcgcgg ccaattaact ccttttcaag agaaggtgtc
    24841 agagggactg cttcaagcag tagacaagca aaagccaacc ttagttcatg cggtaacagg
    24901 agctggaaag acagaaatga tttatcaagt agtggctaaa gtgatcaatg cgggtggtgc
    24961 agtgtgtttg gctagtcctc gcatagatgt ttgtttggag ctgtacaagc gcctgcaaca
    25021 ggatttttct tgcgggatag ctttgctaca tggagaatcg gaaccttatt ttcgaacacc
    25081 actagttgtt gcaacaaccc atcagttatt gaagttttat caagcttttg atttgctgat
    25141 agtggatgaa gtagatgctt ttccttatgt tgataatccc atgctttacc acgctgtcaa
    25201 gaatagtgta aaggagaatg gattgagaat ctttttaaca gcgacttcga ccaatgagtt
    25261 agataaaaag gtccgtttag gagaactaaa aagactgaat ttaccgagac ggtttcatgg
    25321 aaatccgttg attattccaa aaccaatttg gttatcggat tttaatcgct acttagacaa
    25381 gaatcgtttg tcaccaaagt taaagtccta tattgagaag cagagaaaga cagcttatcc
    25441 gttactcatt tttgcttcag aaattaagaa aggggagcag ttagcagaaa tcttacagga
    25501 gcaatttcca aatgagaaaa ttggctttgt atcttctgta acagaggatc gattagagca
    25561 agtacaagct tttcgagatg gagaactgac aatacttatc agtacgacaa tcttggagcg
    25621 tggagttacc ttcccttgtg tggatgtttt cgtagtagag gccaatcatc gtttgtttac
    25681 caagtctagt ttgattcaga ttggtggacg agttggacga agcatggata gaccgacagg
    25741 agatttgctt ttcttccatg atgggttaaa tgcttcaatc aagaaggcga ttaaggaaat
    25801 tcagatgatg aataaggagg ctggtctatg aagtgcttgt tatgtgggca gactatgaag
    25861 actgttttaa cttttagtag tctcttactt ctgaggaata atgactcttg tctttgttca
    25921 gactgtgatt ctacttttga aagaattggg gaagagaact gtccaaattg tatgaaaaca
    25981 gagttgtcaa caaagtgtca agattgtcaa ctttggtgta aagaaggagt tgaagtcagt
    26041 catagagcga tttttactta caatcaagct atgaaggatt ttttcagtcg gtataagttt
    26101 gatggagact tcctgttaag aaaagttttc gcttcatttt taagtgagga gttgaaaaag
    26161 tacaaagagt atcaatttgt tgtaattccc ctaagtcctg atagatatgc taacagagga
    26221 tttaatcagg ttgagggctt ggtagaggca gcaggctttg agtatctgga tttattagag
    26281 aaaagagaag agagagccag ttcttctaaa aatcgttcag agcgcttggg gacagaactt
    26341 cctttcttta ttaaaagtgg agtcactatt cctaaaaaaa tcttacttat agatgatatc
    26401 tatactacag gagcaactat aaatcgtgtt aagaaactgt tggaagaagc tggtgctaag
    26461 gatgtaaaaa cattttccct tgtaagatga ggaaaaaatt tgcaaaaata gaatgaaagc
    26521 gttataataa aatcataaaa acaaaaatgt ttagaaagaa ggtactcata tgattaaata
    26581 tagtatccgt ggtgaaaacc tagaagtaac agaagcaatt cgtgattatg tagtttctaa
    26641 actcgaaaag atcgaaaagt acttccaacc agaacaagag ttggatgccc gaattaactt
    26701 aaaagtttat cgtgaaaaaa cggctaaagt ggaagtaacg attccgcttg gatctattac
    26761 tctccgcgca gaagatgtat ctcaagatat gtatggttca attgaccttg taactgataa
    26821 aattgaacgt cagattcgta aaaataaaac aaaaatcgag cgtaaaaata aaaataaggt
    26881 agcaactggt caattattta cagatgcttt ggtggaagat tcaaatgttg tccagtctaa
    26941 agttgttcgt tcaaaacaaa ttgatttaaa accaatggat ttggaagaag caattctaca
    27001 aatggattta ttggggcatg atttctttat ctatgtagat gttgaagatc agacaaccaa
    27061 tgtgatttat cgtcgtgagg atggtgaaat tggtttgtta gaggttaaag aatcttaatt
    27121 tcaatatgtg taaaggtagg tttactgaat tgtaaactac ctttttctta taattgatag
    27181 aattactgat tacactttta aaaagtcgct ttttgagggg tattatggta taatgggtgc
    27241 caagaggttt tgaatgaaaa aattttatgt aagtccaatt tttcctattc tagtaggatt
    27301 gattgcgttt ggagtcttat ccactttcat tatttttgtt aataataatc tgttgacggt
    27361 tttaattttg tttctttttg taggaggcta tgttttttta tttaagaaac tgagagtgca
    27421 ttatacaagg agtgatgtag aacagataca gtatgtaaac caccaagcgg aagaaagttt
    27481 gacatctcta ttggaacaga tgcctgtagg tgttatgaaa ttgaatttat cttctggaga
    27541 ggttgagtgg tttaatccct atgctgaatt gattttgacc aaggaagatg gtgattttga
    27601 tttggaagct gttcaaacga ttatcaaggc ttcagtagga aatccgtcta cttatgccaa
    27661 gcttggtgag aagcgttatg ctgttcatat ggatgcttct tcaggtgttt tgtattttgt
    27721 agatgtatcc agggaacaag ccataacaga tgaattggta acaagtcgac cagtgattgg
    27781 gattgtctct gtggataatt atgatgattt ggaggatgca acttctgagt cagatattag
    27841 tcaaatcaat agttttgtag ctaattttat atcagagttt tcagaaaaat acatgatgtt
    27901 ttctcgtcgg gtaagtatgg atcgatttta tctatttact gactacacgg tgcttgaggg
    27961 cttgatgaat gataaatttt ctgttattga tgctttcaga gaagagtcga aacagagaca
    28021 gttgcccttg accttaagta tgggattttc ttatggcgat ggaaatcatg atgagatagg
    28081 gaaagttgct ttgctcaatt tgaacttggc tgaagtacgt ggtggcgacc aggtggttgt
    28141 taaggaaaac gacgaaacga aaaatccagt ttattttggt ggtgggtctg ctgcttcaat
    28201 caagcgtaca cggactcgta cgcgcgctat gatgacagct atttcagata agattcggag
    28261 tgtagatcag gtttttgtag tcggtcacaa aaatttagac atggatgctt tgggctctgc
    28321 tgtaggtatg cagttgttcg ccagcaatgt gattgaaaat agctatgctc tttatgatga
    28381 agaacaaatg tctccagata ttgaacgagc tgtttcattc atagaaaaag aaggagttac
    28441 gaagttgttg tctgttaagg atgcaatggg gatggtgacc aatcgttctt tgttgattct
    28501 tgtagaccat tcaaagacag ccttaacatt atcaaaagaa ttttatgatt tatttaccca
    28561 aaccattgtt attgaccacc atagaaggga tcaggatttt ccagataatg cggtcattac
    28621 ttatatcgaa agtggtgcaa gtagtgccag tgagttggta acggaattga ttcagttcca
    28681 gaattctaag aaaaatcgtt tgagtcgtat gcaagcaagt gttttgatgg ctggtatgat
    28741 gttggatact aaaaatttca cctcgcgagt aactagtcgg acatttgatg ttgctagcta
    28801 tctcagaacg cgcggaagtg atagtattgc tatccaggaa atcgctgcga cagattttga
    28861 agaatatcgt gaggtcaatg aacttatttt acaggggcgt aaattaggtt cagatgtact
    28921 aatagcagag gctaaggaca cgaaatgcta tgatacagtt gttattagta aggcagcaga
    28981 tgccatgtta gccatgtcag gtattgaagc gagttttgtt cttgcgaaga atatacaagg
    29041 atttatctct atctcagctc gaagtcgtag taaactgaat gtacaacgga ttatggaaga
    29101 gttaggcggt ggaggccact ttaatttggc agcagctcaa attaaagatg taaccttgtc
    29161 agaagcaggt gaaaaactga cagaaattgt attaaatgaa ataaaggaaa aggagaaaga
    29221 agaatgaaag taatcttttt agcagatgtt aaaggaaaag gtaaaaaagg cgaaattaag
    29281 gaagtaccaa cagggtatgc gcaaaacttt cttatcaaaa agaatctagc caaagaagcg
    29341 actgctcaag ctgtaggtga acttcgtggt aaacaaaaat cggaagaaaa agctcacgct
    29401 gagatgattg cagaaggaaa agcaattaaa gcacaacttg aagcagaaga aactgttgta
    29461 gaatttgttg aaaaagttgg tccagatggt cgtacctttg gttctattac caataagaag
    29521 attgcagaag aattgcaaaa gcaatttgga attaagattg ataaacgtca tattcaagta
    29581 caagctccga ttcgagcggt tggtttgatt gatgtgccag tgaaaatcta tcaagatatc
    29641 acaagtgtaa tcaatcttcg tgtgaaagaa ggataagttt acactttctt gacaagattg
    29701 taaaaggaag ggaagtctga tggcagaagt agaagagtta cgagtacaac ctcaagatat
    29761 cttagctgag caatccgttt taggggctat ctttattgat gagagtaaac ttgtttttgt
    29821 gcgagaatac attgagtctc gggacttttt taagtatgcc catcgtttga ttttccaagc
    29881 catggtcgat ttatccgatc gtggtgatgc catagatgca acaacggttc gtactatcct
    29941 tgataatcaa ggtgatttac agaatattgg tggcttgtct tacttggttg agattgttaa
    30001 ttctgtgcca acttctgcta atgcggagta ttatgctaag attgttgcag aaaaagcaat
    30061 gctacgtcgt ttaattgcca agttgacaga gtctgtcaac caagcttacg aagcgtcaca
    30121 accagctgat gaaattattg ctcaggcaga aaaagggttg attgatgtca gtgaaaatgc
    30181 aaatcgaagc gggtttaaga acattcgaga tgtgttgaat ctcaactttg gaaatctgga
    30241 agctcgctcg caacaaacga ccgatattac aggtattgcg acaggttatc gtgatttgga
    30301 tcatatgaca acaggacttc atgaggagga gttgattatc ttagcagctc gtccagcagt
    30361 tggtaagaca gcatttgcct tgaatatcgc tcagaacatt gggactaagt tggacaaaac
    30421 ggttgctatt ttttcactcg aaatgggtgc ggaaagcttg gtagatcgta tgttagctgc
    30481 agaaggcttg gtggagtcac attctatccg tacagggcaa ttgacagatg aggagtggca
    30541 aaaatatact attgctcagg gtaatctagc taacgccagt atctatatcg atgatacgcc
    30601 aggtattcgg attacagaga ttcgttctcg ttctcgtaaa ttggctcaag aaactggaaa
    30661 tcttggtttg attttgatag actatttgca acttatcacg ggaactggtc gagaaaatcg
    30721 tcaacaagaa gtttctgaaa tttctcgtca gttgaaaata ctagccaagg aattgaaggt
    30781 tccagtaatc gctctgagtc agctttctcg tggtgtagaa caacgtcagg acaagagacc
    30841 ggtcttgtct gatattcgtg aatctgggtc tattgagcag gacgctgata tcgtagcttt
    30901 tctctatcgc gatgactact atgaacgtgg tggtgaagaa gaggagggta tcccaaataa
    30961 taaggtggaa gttattatcg agaaaaaccg tagtggagct cgtggaacag tggaattgat
    31021 tttccaaaaa gaatacaata aattttcaag tatctcaaag agggaggcat aagatgtcag
    31081 atgcatttac agatgtagcc aagatgaaaa aaatcaaaga agaaatcaag gcacatgagg
    31141 gacaagtcgt agaaatgact ttggagaatg gtcgtaagcg ccaaaaaaat agattgggta
    31201 agctaattga agtttatcca tccctattta ttgtggagtt tggggatgtg gaaggagata
    31261 aacaagttaa tgtttacgtt gaatccttta cttactcaga tattcttaca gaaaagaatt
    31321 tgattcatta tcttgactaa agtgagaaat tttctcactt tttctttttt ctccgaataa
    31381 tttaggtgaa ggcaatcatc gctttatatt atttttcaag gaggaagaat gaaaatttta
    31441 ccgtttatag caagaggaac aagttattac ttgaagatgt cagttaaaaa gcttgttcct
    31501 tttttagtag taggattgat gctagcagct ggtgatagtg tctatgccta ttccagagga
    31561 aatggatcga ttgcgcgtgg ggatgattat cctgcttatt ataaaaatgg gagccaggag
    31621 attgatcagt ggcgcatgta ttctcgtcag tgtacttctt ttgtagcctt tcgtttgagt
    31681 aatgtcaatg gttttgaaat tccggcagct tatggaaatg cgaatgaatg gggacatcgt
    31741 gctcgtcggg aaggttatcg tgtagataat acaccgacga ttggttccat tacttggtct
    31801 actgcaggaa cttatggtca tgttgcctgg gtgtcaaatg taatgggaga tcagattgag
    31861 attgaggaat ataactatgg ttatacagaa tcctataata aacgagttat aaaagcaaac
    31921 acgatgacag gatttattca ttttaaagat ttggatggtg gcagtgttgg gaatagtcaa
    31981 tcctcaactt caacaggcgg aactcattat tttaagacca agtctgctat taaaactgaa
    32041 cctctagcta gcggaactgt gattgattac tattatcctg gggagaaggt tcattatgat
    32101 cagatacttg aaaaagacgg ctataagtgg ttgagttata ctgcctataa tggaagctat
    32161 cgttatgttc aattggaggc tgtgaataaa aatcctctag gtaattctgt tctttcttca
    32221 acaggtggaa ctcattattt taagaccaag tctgctatca aaactgaacc cctagttagt
    32281 gcaactgtga ttgattacta ttatcctgga gagaaggttc attatgatca aattctcgaa
    32341 aaagacggct acaagtggtt gagttatacg gcttataacg gaagtcgtcg ctatatacag
    32401 ctagagggag tgacttcttc acaaaattat cagaatcaat caggaaatat ctctagctat
    32461 ggatccaata atagttcaac tgtcggttgg aagaaaataa atggtagttg gtatcatttc
    32521 aaatcaaatg gttctaaatc aacaggatgg ctgaaagacg gttctagctg gtattatttg
    32581 aaattatctg gtgaaatgca gacaggatgg ttaaaggaga atggctcgtg gtattatctg
    32641 ggtagttcag gggcaatgaa aacaggctgg taccaggtct ctggtaagtg gtattattct
    32701 tactcttcag gcgccttagc tgttaatacg acggtggatg gctacagagt aaacagtgat
    32761 ggagaacgag tatagaaaat tggagtagga gaaatttcct gctcttttct gtaagcagtt
    32821 tccttgacat ttttcttatt ttgcgctatc atatatacat ataatatatg ggagtctgtg
    32881 tacagtctga gaggaagtgt taaacttcga ccgcacctga tctggataat gccagcggag
    32941 ggaacgatac ttagtctaat tttgcacctt ttccatgtat ggtaaaggtt tttctttttt
    33001 ttaaaaggaa aacgagaaga ggaggttctt atgaaagcaa gcattgcctt gcaagtttta
    33061 cccctagcac aggggattga tcggatagct gttattgatc aggtcattgc ttatctgcaa
    33121 actcaagaag tgacgatggt agtgacacca tttgaaacgg tcttggaagg ggagtttgat
    33181 gagcttatgc gcattctaaa agaagcgctg gaagtggcag ggcaggaggc agacaatgtc
    33241 tttgccaatg tcaaaataaa tgtaggagag attttaagta ttgatgagaa acttgaaaag
    33301 tatactgaga cgacacatta gtctattggg ctttctcgga gtattgtcaa tctggcagtt
    33361 agcaggtttt cttaaacttc tccccaagtt tatcctgccg acacctcttg aaattctcca
    33421 gccctttgtt cgtgacagag aatttctctg gcaccatagc tgggcgacct tgagagtggc
    33481 tttactgggg ctgattttgg gagttttgat tgcctgtctt atggctgtgc tcatggatag
    33541 tttgacttgg ctcaatgacc tgatttaccc tatgatggtg gtcattcaga ccattccgac
    33601 cattgccata gctcctatcc tggtcttgtg gctaggttat gggattttgc ccaagattgt
    33661 cttgattatc ttaacaacaa cctttcccat catcgttagt attttggacg gttttaggca
    33721 ttgtgacaag gatatgctga ctttgtttag tctgatgcga gcaaaccctt ggcaaatcct
    33781 gtggcatttt aaaatcccgg ttagcctgcc ttacttttat gcaggtctga gggtcagtgt
    33841 ctcctacgcc tttatcacaa ctgtggtatc tgagtggttg ggaggttttg aaggtcttgg
    33901 tgtttatatg attcagtcta aaaaactgtt tcagtatgat accatgtttg ccattattat
    33961 tctggtgtcg attatcagtc ttttgggtat gaagctggtc gatatcagtg aaaaatatgt
    34021 gattaaatgg aaacgtactt aaaatagagc gtttttgaaa aagaaaagag gaaatcaaaa
    34081 tgaagaaaac atggaaagtg tttttaacgc ttgtaacagc tcttgtagct gttgtgcttg
    34141 tggcctgtgg tcaaggaact gcttctaaag acaacaaaga ggcagaactt aagaaggttg
    34201 actttatcct agactggaca ccaaatacca accacacagg gctttatgtt gccaaggaaa
    34261 aaggttattt caaagaagct ggagtggatg ttgatttgaa attgccacca gaagaaagtt
    34321 cttctgactt ggttatcaac ggaaaggcac catttgcagt gtatttccaa gactacatgg
    34381 ctaagaaatt ggaaaaagga gcaggaatca ctgccgttgc agctattgtt gaacacaata
    34441 catcaggaat catctctcgt aaatctgata atgtaagcag tccaaaagac ttggttggta
    34501 agaaatatgg gacatggaat gacccaactg aacttgctat gttgaaaacc ttggtagaat
    34561 ctcaaggtgg agactttgag aaggttgaaa aagtaccaaa taacgactca aactcaatca
    34621 caccgattgc caatggcgtc tttgatactg cttggattta ctacggttgg gatggtatcc
    34681 ttgctaaatc tcaaggtgta gatgctaact tcatgtactt gaaagactat gtcaaggagt
    34741 ttgactacta ttcaccagtt atcatcgcaa acaacgacta tctgaaagat aacaaagaag
    34801 aagctcgcaa agtcatccaa gccatcaaaa aaggctacca atatgccatg gaacatccag
    34861 aagaagctgc agatattctc atcaagaatg cacctgaact caaggaaaaa cgtgactttg
    34921 tcatcgaatc tcaaaaatac ttgtcaaaag aatacgcaag cgacaaggaa aaatggggtc
    34981 aatttgacgc agctcgctgg aatgctttct acaaatggga taaagaaaat ggtatcctta
    35041 aagaagactt gacagacaaa ggcttcacca acgaatttgt gaaataatga cagaaattag
    35101 actagagcac gtcagttatg cctatggtca ggagaggatt ttagaggata tcaacctaca
    35161 ggtgacttca ggcgaagtgg tttccatcct aggcccaagt ggtgttggaa agaccaccct
    35221 ctttaatcta atcgctggga ttttagaagt tcagtcaggg agaattgtcc ttgatggtga
    35281 agaaaatccc aaggggcgcg tgagttatat gttgcaaaag gatctgctct tggagcacaa
    35341 gacggtgctt ggaaatatca ttctgcccct cttgattcaa aaggtggata aggcagaagc
    35401 tatttcccga gcggataaaa ttcttgcgac cttccagctg acagctgtaa gagacaagta
    35461 tcctcatgaa cttagcggtg ggatgcgcca gcgtgtagcc ttactccgga cctacctttt
    35521 tgggcacaag ctctttctct tagatgaggc ctttagcgcc ttggatgaga tgacaaagat
    35581 ggaactccac gcttggtatc ttgagattca caagcagttg cagctaacaa ccctgatcat
    35641 cacgcatagt attgaggagg ccctcaatct cagcgaccgc atctatatct tgaaaaatcg
    35701 ccctgggcag attgtttcag aaattaaact agattggtct gaagatgagg acaaggaagt
    35761 ccaaaagatt gcctacaaac gtcaaatttt ggcggaatta ggcttagata agtagaaaaa
    35821 tagggagttg gtgaagatta tcctttacca gcgccctttt tcttttaaaa atgagaaaat
    35881 ttcggtataa tagtcaaaca aggtcaaggt ttaaagagag aggtgggttt gttatgagat
    35941 ttaaaaatac atcggatcat attgaggcct acatcaaggc gattttagat caatctggta
    36001 tcgtggagtt gcaacggagt cagttggcag atacctttca ggttgttcct agtcagatta
    36061 actacgtgat caagacacgc tttacggaaa gtagaggcta cttggttgaa agtaagcgtg
    36121 gtggcggagg ctacattcgt ataggacgga ttgagttttc tagtcatcat gaaatgctcc
    36181 gggagctgct ttactcgatt ggtgagcgag tcagtcaaga aatttatgag gatattctcc
    36241 agcttttggt tgagcaggaa ttgatgacca agcaggagat gaatttgcta gaatcagtag
    36301 ctttggatcg cgttttagga gaagaagctc cagttgttcg agcaaacatg ctacgtcaga
    36361 tcatacaaga ggtagataga aaagggaagt aagatgaact attcaaaagc attgaatgaa
    36421 tgtatcgaaa gtgcctacat ggttgctgga cattttggag ctcgttatct agagtcttgg
    36481 cacttgttga ttgccatgtc taatcacagt tatagtgtag caggggcaac tttaaatgat
    36541 tatccgtatg agatggaccg tttagaagag gtggctttgg aactgactga aacggactat
    36601 agccaggatg aaacctttac ggaattgccg ttctcccgtc gtttgcaggt tctttttgac
    36661 gaagcagagt atgtagcgtc agtggtccat gctaaggtgc tagggacaga gcacgtcttc
    36721 tatgcgattt tgcatgatgg caatgccttg gcaactcgta tcttggagag ggctggtttt
    36781 tcttatgaag acaagaaaga tcaggtcaag attgctgccc ttcgtcgaaa tttagaagaa
    36841 cgggcaggct ggactcgtga agatctcaag gctttacgcc aacgccatcg tacagtagct
    36901 gacaagcaaa attctatggc caatatgatg ggcatgccgc agactcctag tggtggtctt
    36961 gaggactata cgcatgattt gacagagcaa gcgcattctg gcaagttaga accagtcatc
    37021 ggtcgggaca aggaaatctc acgtatgatt caaatcttga gccgtaagac taagaacaac
    37081 cctgtcttgg ttggggatgc tggtgtcggg aaaacagctc tggcccttgg tcttgcccag
    37141 cgtattgcta gtggtgacgt gcctgcggaa atggctaaga tgcgcgtgtt agaacttgat
    37201 ttgatgaatg tcgttgcagg gacacgcttc cgtggtgact ttgaagaacg catgaataat
    37261 atcatcaagg atattgaaga agatggccaa gtcatcctct ttatcgatga actccacacc
    37321 atcatgggtt ctggtagcgg gattgattcg actctggatg cggccaatat cttgaaacca
    37381 gccttggcgc gtggaacttt gagaacggtt ggtgccacca ctcaggaaga atatcaaaaa
    37441 catatcgaaa aagatgcggc actttctcgt cgtttcgcta aagtgacgat tgaagaacca
    37501 agtgtggcag atagtatgac tattttacaa ggtttgaagg cgacttatga gaaacatcac
    37561 cgtgtacaaa tcacagatga agcggttgaa acagcggtta agatggctca tcgttattta
    37621 accagtcgtc acttgccaga ctctgctatc gacctcttgg acgaagcagc agcaacagtg
    37681 caaaataagg caaagcatgt aaaagcagac gattcagatt tgagtccagc tgacaaggcc
    37741 ctgatggatg gcaagtggaa acaggcagcc cagctaatcg caaaagaaga ggaagtacct
    37801 gtctacaaag acttggtgac agagtctgat attttgacca ccttgagtcg cttgtcagga
    37861 atcccagttc aaaaactgac tcaaacggat gctaagaagt atctaaatct tgaagcagaa
    37921 ctccataaac gggttatcgg tcaagatcaa gctgtttcaa gcattagtcg tgcgattcgc
    37981 cgtaatcagt caggaattcg cagtcacaag cgtccgatcg gttcctttat gttcctagga
    38041 cctacaggtg tcggtaagac cgagttggcc aaagctttgg cagaagttct ctttgatgaa
    38101 gaatcagccc ttatccgctt tgatatgagt gagtatatgg agaaattcgc agccagccgt
    38161 ctaaatggag ctcctccagg ctatgtgggt tacgaagaag gtggggagtt gacagagaag
    38221 gttcgcaata aaccctattc cgttctcctc tttgatgagg tagagaaggc ccacccagat
    38281 atctttaatg ttctcttgca ggttctggat gacggtgtct tgacagatag caagggacgc
    38341 aaggtcgatt tttcaaatac cattatcatt atgacatcga acctaggtgc gactgccctt
    38401 cgtgatgata agactgttgg ttttggggct aaggatattc gttttgacca ggaaaatatg
    38461 gaaaaacgca tgtttgaaga actgaaaaaa gcttatagac cggaattcat caaccgtatt
    38521 gatgagaagg tggtcttcca tagcctatct agtgaccata tgcaggaagt ggtgaagatt
    38581 atggtcaagc ctttagtggc aagtttggct gaaaaaggca ttgacttgaa attacaagct
    38641 tcagctctga aattgttagc aaatcaagga tatgacccag agatgggagc tcgcccactt
    38701 cgcagaaccc tgcaaacaga agtggaggac aagttggcag aacttcttct taagggagat
    38761 ttagtggcag gcagcacact taagattggt gtcaaagcag gccagttaaa atttgatatt
    38821 gcataaaaga ataaaagtat cagcatctga ccataagtca cagtggagtg aaactcaatg
    38881 aaaatcaaag agcaaactag gcagctagcc gcaggttgct caaaacactg ttttgaggtt
    38941 gtagatagag ctaacgtggt ttgaagagat tttcgaagag tataagttgc aagatgaatg
    39001 attttcttgt attgacgttg ttgttgacaa aaagtagcgg ataaatgaaa tccattccat
    39061 tatcatagat gataggctgg taggaaattt tcaaatagca tacaggaaat agatgtatgg
    39121 agttctggta gtagaaaggg agagagatga acattttagt tgcagatgac gaggaaatga
    39181 ttagagaagg aattgcagca tttctgacag aagagggtta tcatgtcatt atggctaagg
    39241 atggacaaga ggtcttggaa aaatttcaag atctccctat ccatctcatg gtactggatt
    39301 taatgatgcc taggaagagt ggttttgaag tgttaaaaga aatcaatcaa aagcacgata
    39361 ttcctgtcat cgtcttgagt gctctgggag atgaaactac tcagtcacag gtatttgatc
    39421 tctatgctga tgatcatgtg acaaaacctt tttctttggt actgcttgtc aagcgtatta
    39481 aggcgcttat cagacgttac tacgtcatag aggatctttg gcgatatcag gatgtaacag
    39541 tggattttac ctcttacaaa gcacattata aaaatgaaga aattgatctc aaaccaaagg
    39601 aattactggt actaaagtgt ttgattcagc ataaaaatca agttttaagt agagagcaga
    39661 tattggaaga aatttcaaaa gatgtagctg atttaccttg tgatagggtc gttgatgtct
    39721 atattcgtac tcttcgcaaa aaattagctt tagattgtat cgtgactgtg aaaaatgttg
    39781 ggtataagat tagcttatga taaaaaatcc taaattatta accaagtctt ttttaagaag
    39841 ttttgcaatt ctaggtggtg ttggtctagt cattcatata gctatttatt tgacctttcc
    39901 tttttattat attcaactgg agggggaaaa gtttaatgag agcgcaagag tgtttacgga
    39961 gtatttaaag actaagacat ctgatgaaat tccaagctta ctccagtctt attcaaagtc
    40021 cttgaccata tctgctcacc ttaaaagaga tattgtagat aagcagctcc ctcttgtgca
    40081 tgacttggat attaaagatg gaaagctatc aaattacatc gtgatgttag atatgtctgt
    40141 tagtacagca gatggtaaac aggtaacagt gcaatttgtt cacggggtga atgtctacaa
    40201 agaagcaaag aatattttgc ttttgtatct cccatataca tttttggtta caattgcttt
    40261 ttcctttgtt ttttcttatt tttatactaa acgcttgctc aatcctcttt tttacatttc
    40321 agaagtgact agtaaaatgc aagatttgga tgacaatatt cgttttgatg aaagtaggaa
    40381 agatgaagtt ggtgaagttg gtgaagttgg aaaacagatt aatggtatgt atgagcactt
    40441 gttgaaggtt attcatgagt tggaaagtcg taatgagcaa attgtaaaat tgcaaaatca
    40501 aaaggtttcc tttgtccgcg gagcatcaca tgagttgaaa acccctttag ccagtcttag
    40561 aattatccta gagaatatgc agcataatat tggagattac aaagatcatc caaaatatat
    40621 tgcaaagagt ataaataaga ttgaccagat gagccactta ttagaagaag tactggagtc
    40681 ttctaaattc caagagtgga cagagtgtcg tgagaccttg actgttaagc cagttttagt
    40741 agatatttta tcacgttatc aagaattagc tcattcaata ggtgttacaa ttgaaaatca
    40801 attgacagat gctaccaggg tcgtcatgag tcttagggca ttggataagg ttttgacaaa
    40861 cctgattagt aatgcaatta aatattcaga taaaaatggg cgtgtaatca tatccgagca
    40921 agatggctat ctctctatca aaaatacatg tgcgcctcta agtgaccaag aactagaaca
    40981 tttatttgat atattctatc attctcaaat cgtgacagat aaggatgaaa gttccggttt
    41041 gggtctttac attgtgagta atattttaga aagctatcaa atggactata gttttctccc
    41101 ttatgaacac ggtatggaat ttaagattag cttgtagaca gattagtttt ttattaaagt
    41161 tcacataggg ttaacataag tgtattattc tttatataga taaaaatata gataaaagaa
    41221 aggatactaa tatggtatta gcgattattt tagtaacatt ccttattcga ttgatttttt
    41281 taaagcgttc gatagagaat gagaaacgaa tccttagcaa tggcgggaaa gaatttggag
    41341 ttgagaatac aaaacgatta acgttggctc atattgtttt ttatctctct tgcctcgttg
    41401 aagcaatggt gcataagaca atgtttgatg gtatgagcat ggttggttta gtcttgctta
    41461 ttttttctat gctgatgttg atgttggtga ttcacttgtt gggagatatt tggacagtga
    41521 agcttatgct tgtcaataat cacaaatatg tagatcatat cttgtttagg acagtaaaac
    41581 atcctaatta ctttttaaat attttacctg agttgattgg cttgaccttg ttgagtcatg
    41641 cctatatgac ttttgtttta gtttttccag tttatgcagt tattttgtat cgacgaatag
    41701 ctgaagagga aaagctatta catgaagtta taatcccaaa tggaagcata aagagataaa
    41761 tacaaaattc gatttatata cagttcatat tgaagtgata tagtaaggtt aaagaaaaaa
    41821 tatagaagga aataaacatg tttgcatcaa aaagcgaaag aaaagtacat tattcaattc
    41881 gtaaatttag tattggagta gctagtgtag tagttgctag tttgttctta ggaggagtag
    41941 ttcatgcaga agaggttaga agagggaata acctcacggt tacatctagt ggggatgaag
    42001 tcgagtcgca ttatcaaagt atattggaga aggtcagaaa aagtttggaa aaagatcgac
    42061 atacccaaaa tgtcgactta atcaaaaagt tgcaagacat taagagaacg tatttgtata
    42121 atttaaaaga gaagccggaa gctgagttga cgtcaaaaac aaaaaaagag ttagacgcag
    42181 cttttgagaa gtttaaaaaa gaaccagaac ttactaaaaa attagcagaa gctgagaaga
    42241 aggttgcaga agctgagaaa aaagccaagg atcaaaaaga agaagatcac cgtaactacc
    42301 caaccaatac ttacaaaaca atcgaactgg aaattgcgga agcagaagta ggggtcgcca
    42361 aggcagagct tgagcttgca caagctcaag tccaaatacc tcaagatact gagaaaatta
    42421 atgctgctaa agctaaagta gaagctgcta aaagtaatgt taaaaaacta gaaaaaatta
    42481 aatcagatat tgaaaaaacg tatttgtata aattagataa ctcaaccaaa aaaacgccaa
    42541 aacctagagt gcgaagaaat tctccggaaa taaaagctaa gggtagagtc aaaaattata
    42601 aagaggctaa tattgaactt tctaaatata tgactgattt gtataaatta gataactcaa
    42661 ccaaagaaac gccaaaatct agagtgcgaa gaaattctcc gcaagtaggc gattcgagag
    42721 aacttaagga aacgatagac aaagcgaaag aaactctgtc tacctatatg gtaactcgtt
    42781 taacgaagct ggatccatct gttttttggt ttgcagatct tcttatggat gctaagaagg
    42841 ttgtggaaga atacaagaca aaattagagg atgcttcaga taaaaaatcg gtagaagact
    42901 tgcgaaagga agcagaagga aaaatagagt ctcttatcgt gactcaccaa aatagagaaa
    42961 aagaaaacca accagcaccc caaccaggag gacaagcagg tggttcaatg gttgtaccac
    43021 cggtgacgca aacacctcca tcaacttccc aaagtccagg acaaaaggcg accgaagctg
    43081 aaaagaaaaa gttacaagac ttgattcgtc aattccaaga agccttgaac aaactagacg
    43141 atgaaacaaa gactgttcca gatggggcta aactcacagg agaagctgga aaagcctata
    43201 atgagactag aacttatgcg aaagaagttg ttgacaaaag caagaagctt ctatcacaga
    43261 cagcagtgac aatggatgaa ttggcaatgc aattaaccaa attgaacgat gccatgtcta
    43321 aattgaaaga agctaaagcg aaattggtac cagaggttaa accacagccg gaaaacccag
    43381 agccaaaacc acaaccagag ggtgagaaac caagcgtacc agatattaat caggagaaag
    43441 aaaaagctaa acttgctata gcaacataca tgagcaagat tttagatgat ataaagaaac
    43501 atcatctgaa gaaagaaaaa catcatcaga ttgttgctct tattaaggac cttgataaac
    43561 ttaaaaagca agcactttct gaaattgata atgtaaatac caaagtagaa attgagaata
    43621 cagtccacaa ggtatttgca gacatggata cggttgttac taaattccaa aaaggcttaa
    43681 ttcagaacac accgcaggtt ccagaagcac caaagagccc agaggtacca aaggtttcag
    43741 atacaccaaa ggctccggac acaccgcagg ttccagaagc accaaagagc ccagaggtac
    43801 caaaggttcc agaa
//
LOCUS       DAVMAF010000009        40145 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMAF010000009 DAVMAF010000000
VERSION     DAVMAF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.009"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            72..302
                     /gene="blpU"
                     /locus_tag="VYO97_000462"
     CDS             72..302
                     /gene="blpU"
                     /locus_tag="VYO97_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1753321.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGVARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1017..3170
                     /locus_tag="VYO97_000463"
     CDS             1017..3170
                     /locus_tag="VYO97_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1753322.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKM
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3183..4532
                     /locus_tag="VYO97_000464"
     CDS             3183..4532
                     /locus_tag="VYO97_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1753323.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTVKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAEINLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4702..5409
                     /locus_tag="VYO97_000465"
     CDS             4702..5409
                     /locus_tag="VYO97_000465"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1753324.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFTEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5611..9336
                     /locus_tag="VYO97_000466"
     CDS             5611..9336
                     /locus_tag="VYO97_000466"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1753325.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNISE
                     ANILFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTVEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9429..10871
                     /locus_tag="VYO97_000467"
     CDS             9429..10871
                     /locus_tag="VYO97_000467"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1753326.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10908..11930
                     /locus_tag="VYO97_000468"
     CDS             10908..11930
                     /locus_tag="VYO97_000468"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1753327.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11927..12472
                     /gene="purN"
                     /locus_tag="VYO97_000469"
     CDS             11927..12472
                     /gene="purN"
                     /locus_tag="VYO97_000469"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1753328.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYIKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12556..13065
                     /locus_tag="VYO97_000470"
     CDS             12556..13065
                     /locus_tag="VYO97_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1753329.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13090..14637
                     /gene="purH"
                     /locus_tag="VYO97_000471"
     CDS             13090..14637
                     /gene="purH"
                     /locus_tag="VYO97_000471"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1753330.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14759..16021
                     /gene="purD"
                     /locus_tag="VYO97_000472"
     CDS             14759..16021
                     /gene="purD"
                     /locus_tag="VYO97_000472"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1753331.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16424..16912
                     /gene="purE"
                     /locus_tag="VYO97_000473"
     CDS             16424..16912
                     /gene="purE"
                     /locus_tag="VYO97_000473"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1753332.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16899..17990
                     /gene="purK"
                     /locus_tag="VYO97_000474"
     CDS             16899..17990
                     /gene="purK"
                     /locus_tag="VYO97_000474"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1753333.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            18000..18227
                     /locus_tag="VYO97_000475"
     CDS             18000..18227
                     /locus_tag="VYO97_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1753334.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18290..19588
                     /gene="purB"
                     /locus_tag="VYO97_000476"
     CDS             18290..19588
                     /gene="purB"
                     /locus_tag="VYO97_000476"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1753335.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19643..23581)
                     /gene="strH"
                     /locus_tag="VYO97_000477"
     CDS             complement(19643..23581)
                     /gene="strH"
                     /locus_tag="VYO97_000477"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1753336.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23896..24612)
                     /locus_tag="VYO97_000478"
     CDS             complement(23896..24612)
                     /locus_tag="VYO97_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1753337.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24960..26747
                     /locus_tag="VYO97_000479"
     CDS             24960..26747
                     /locus_tag="VYO97_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1753338.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPTYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26744..27220
                     /locus_tag="VYO97_000480"
     CDS             26744..27220
                     /locus_tag="VYO97_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1753339.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27248..28153
                     /locus_tag="VYO97_000481"
     CDS             27248..28153
                     /locus_tag="VYO97_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1753340.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28140..28955
                     /locus_tag="VYO97_000482"
     CDS             28140..28955
                     /locus_tag="VYO97_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1753341.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28962..29366
                     /locus_tag="VYO97_000483"
     CDS             28962..29366
                     /locus_tag="VYO97_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753342.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29619..30785
                     /locus_tag="VYO97_000484"
     CDS             29619..30785
                     /locus_tag="VYO97_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1753343.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30901..31938
                     /locus_tag="VYO97_000485"
     CDS             30901..31938
                     /locus_tag="VYO97_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1753344.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            32146..33000
                     /locus_tag="VYO97_000486"
     CDS             32146..33000
                     /locus_tag="VYO97_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1753345.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKRLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            33137..33394
                     /locus_tag="VYO97_000487"
     CDS             33137..33394
                     /locus_tag="VYO97_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1753346.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33436..33798)
                     /locus_tag="VYO97_000488"
     CDS             complement(33436..33798)
                     /locus_tag="VYO97_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753347.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFKDFLQLLLTHIKLF"
     gene            complement(33852..34148)
                     /locus_tag="VYO97_000489"
     CDS             complement(33852..34148)
                     /locus_tag="VYO97_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1753348.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(34138..34299)
                     /locus_tag="VYO97_000490"
     CDS             complement(34138..34299)
                     /locus_tag="VYO97_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1753349.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            34369..35133
                     /locus_tag="VYO97_000491"
     CDS             34369..35133
                     /locus_tag="VYO97_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1753350.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(35373..35525)
                     /locus_tag="VYO97_000492"
     CDS             complement(35373..35525)
                     /locus_tag="VYO97_000492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753351.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            35743..37122
                     /locus_tag="VYO97_000493"
     CDS             35743..37122
                     /locus_tag="VYO97_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1753352.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            37136..37801
                     /locus_tag="VYO97_000494"
     CDS             37136..37801
                     /locus_tag="VYO97_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1753353.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            38111..38650
                     /locus_tag="VYO97_000495"
     CDS             38111..38650
                     /locus_tag="VYO97_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1753354.1"
                     /translation="MKLLSIAIPSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            38717..39127
                     /locus_tag="VYO97_000496"
     CDS             38717..39127
                     /locus_tag="VYO97_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1753355.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTTEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            39243..>40145
                     /gene="pavB"
                     /locus_tag="VYO97_000497"
     CDS             39243..>40145
                     /gene="pavB"
                     /locus_tag="VYO97_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1753356.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETV"
BASE COUNT        11086 a         7772 c         9401 g        11886 t
ORIGIN      
        1 agatactgtt atactaaact tgtcaagttt gtaacaagac aaatattaaa aataaaaaag
       61 aggtattcgt tatgaataca aaaaagatgt cacaatttga aattatggat actgagatgc
      121 ttgcttgcgt tgaaggtggc ggatgcaatt ggggagattt tgccaaagca ggtgttggag
      181 gaggagtagc acgaggtctt cagctaggaa ttaaaacaag aacatggcaa ggtgcagcaa
      241 ctggtgctgc gggaggagct atacttggag gtgtggccta tgcagcgaca tgttggtggt
      301 aattatggat tttaaaagtt ttattattgg tttagtagtt ggtatatttg gtccttatat
      361 ggatgattta attagaaaaa aatttttaaa gtcttcggag aagaaaacag aaaaatctgt
      421 taaaaaataa tcaaaactat aaatgatgaa tctgaatcaa aattattttg cgcatgtaaa
      481 gaggagtctt atagtaacga gtcaaaaaag gagtaactat gaatcgtgat ttagaacggt
      541 gttatctatt ctgactagga atagatcata ccagaggtag cttagaaata gcagggacat
      601 tagaaattga agtaataaat aggatgtcgt aagtgttact atcaatgatt tatttgtttc
      661 aagcttgcct agggtgacag taaaaaatca atttcctttc aatagcatat ttttagtggg
      721 caggactctt gttctgccta tttttttatc caaaaagtgc agttgggagg gagataggct
      781 catttgggaa ggaagtccag tttttgttta gtgattgagg taagatagtt gttatcagat
      841 gagtttatac tcttcgaaaa tcaaattcaa accacgtcaa cgtcgccttg acgtatatat
      901 gtgactgact tcgtcagtcc tatctacaac ctcaaaacag tgttttgagc agcctgcggc
      961 tagtttccta gtttgctctt tgattttcat tgagtattag ggaaaaggag atgaatatga
     1021 aatttgggaa acgtcactat cgtccgcaag tggatcagat ggactgcggt gtagcttcat
     1081 tagccatggt ttttggctac tatggtagtt attatttttt ggctcacttg cgagaattgg
     1141 ctaagatgac catggatggg acgacggctt tgggcttggt caaggtggca gaggagattg
     1201 gttttgagac gcgagccatt aaggcggata tgacgctttt tgacttgccg gatttgactt
     1261 ttccttttgt tgcccatgtg cttaaggaag ggaaattgct ccactactat gtggtgactg
     1321 ggcaggataa ggatagcatt catattgccg atccagatcc cggggtgaag ttgactaaac
     1381 tgccacgtga gcgttttgag gaagaatgga caggagtgac tctttttatg gcgcctagtc
     1441 cagactataa gcctcataag gaacaaaaaa atggtctgct ctcttttatc cctatattag
     1501 tgaagcagcg tggcttgatt gccaatatcg ttttggcaac actcttggta accgtgatta
     1561 acattgtggg ttcttattat ctgcagtcta tcattgatac ctatgtgcca gatcagatgc
     1621 gttcgacact agggattatt tctattgggc tagtcatcgt ctacatcctc cagcaaatct
     1681 tgtcttacgc tcaggagtat ctcttgcttg ttttagggca acgcttgtcg attgacgtga
     1741 ttttgtccta tatcaagcat gtttttcacc tccctatgtc cttctttgcg acacgcagga
     1801 caggggaaat tgtgtctcgt ttcacggatg ctaacagtat catcgatgcg ctggcttcga
     1861 ccatcctttc gattttccta gatgtgtcaa cggttgtcat tatttccctt gttctatttt
     1921 cacaaaatac caatctcttt ttcatgactt tattggcgct tcctatctac acagtgatta
     1981 tctttgcctt tatgaagccg tttgaaaaga tgaatcggga taccatggaa gccaatgcgg
     2041 ttctgtcttc ttctatcatt gaggacatca acggtattga gactatcaag tccttgacca
     2101 gtgaaagtca gcgttaccaa aaaattgaca aggaatttgt ggattatctg aagaaatcct
     2161 ttacctatag tcgagcagag agtcagcaaa aggctctgaa aaaggttgcc catctcttgc
     2221 ttaatgtcgg cattctctgg atgggggctg ttctggtcat ggatggcaag atgagtttgg
     2281 ggcagttgat tacctataat accttgctgg tttactttac caatcctttg gaaaatatca
     2341 tcaatctgca aaccaagctt cagacagcgc aggttgccaa taaccgtcta aatgaagtgt
     2401 atctagtagc ttctgagttt gaggagaaga aaacagttga ggatttgagc ttgatgaagg
     2461 gagatatgac cttcaagcag gttcattaca agtatggcta tggtcgagac gtcttgtcgg
     2521 atatcaattt aaccgttccc caagggtcta aggtggcttt tgtggggatt tcagggtcag
     2581 gtaagacgac tttggccaag atgatggtta atttttatga cccaagtcaa ggggagatta
     2641 gtctgggtgg tgtcaatctc aatcagattg ataaaaaagc cctgcgccag tacatcaact
     2701 atctgcctca acagccctat gtctttaacg gaacgatttt ggagaatctt cttttgggag
     2761 ccaaggaggg gacgacacag gaagatatct tacgggcggt cgaattggca gagattcgag
     2821 aggatatcga gcgcatgcca ctgaattacc agacagaatt gacttcggat ggggcaggga
     2881 tttcaggtgg tcaacgtcag agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg
     2941 tcttgatttt ggatgaggcg actagcagtt tggatatttt gacagagaag cggattgtcg
     3001 ataatctcat agctttggac aagaccttga ttttcattgc tcaccgcttg actattgctg
     3061 agcggacaga gaaggtagtt gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg
     3121 ctgatttgct tgcacagggt ggcttttacg cccatttggt caatagctag aaagaggaga
     3181 ggatgaaacc agaattttta gaaagtgcgg agttttataa tcgtcgttac cataattttt
     3241 ccagtagtgt gattgtaccc atggcccttc tgctcgtgtt tttacttggc tttgcaactg
     3301 ttgcagaaaa ggagatgagt ttgtccacta gagctactgt cgaacctagt cgtatccttg
     3361 caaatatcca gtcaactagc aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc
     3421 tggttaagaa gggggatctt ttggttcaat accaagaagg ggcagagggt gtccaagcgg
     3481 agtcctatgc cagtcagttg gacatgctaa aggatcaaaa aaagcaattg gagtatctgc
     3541 aaaagagcct gcaagaaggg gagaaccact ttccagagga ggataagttt ggctaccaag
     3601 ccacctttcg cgactacatc agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa
     3661 atgagaccat cgcgtcccag aatgcagcag ctagccaaac ccaagccgaa atcggcaacc
     3721 tcatcagtca aacagaggct aaaattcgcg attaccagac agttaagtca gctattgaaa
     3781 caggtgcttc cttggccggt cagaatctag cctactctct ttaccagtcc tacaagtctc
     3841 agggcgagga aaatccccaa actaaggttc aggcagttgc acaggttgaa gcacagattt
     3901 ctcagttaga atctagtctt gctacttacc gtgtccagta tgcaggttca ggtacccagc
     3961 aagcctatgc gtcagggtta agcagtcaat tggaatccct taaatcccaa cacttggcaa
     4021 aggttggtca ggaattgacc cttctagccc agaaaatctt ggaggcagag tcaggtaaga
     4081 aggtacaggg aaatctttta gacaagggga aaattacggc aagtgaggat ggggtgcttc
     4141 atcttaatcc tgagaccagt gattctagca tggttgcaga aggtgcccta ctagcccaac
     4201 tttatccatc tttggaaaga gaagggaaag ccaaactcac agcctatcta agttcaaaag
     4261 atgtagcaag aatcaaggtc ggtgattctg ttcgctatac tacgactcat gatgccggga
     4321 atcaactttt tctagattct actattacaa gtattgatgc gacagctact aagactgaga
     4381 aagggaattt ctttaaaatc gaggcggaga ttaatctaac ttcggagcag gctgaaaaac
     4441 ttaggtacgg ggtggaaggc cgtctacaga tgattacggg caagaaaagt tatctacgtt
     4501 attatttgga tcaatttttg aacaaagagt aatgttcgtg tttttagagt taaataattt
     4561 ttaaactgtg agaaagattc ttcttgcagt tttttcttta caatttttga aaaacatctg
     4621 ctatttattc ggttaaattc ttgtgttttt tggttttttg tggtaaaatg tgctcaagta
     4681 atacgaaagg cgaactttaa aatgtcaaaa caattgatct attcgggaaa agctaaagat
     4741 atctatacaa ctgaggatga aaatcttatt atttcaactt acaaggacca ggcgactgct
     4801 ttcaacggtg tcaagaagga gcagattgca ggtaagggag tcttgaataa tcagatctca
     4861 tcttttattt ttgagaaatt aaatgcggct ggtgtggcga ctcactttgt ggagaaactt
     4921 tcagacacgg aacaactcaa taaaaaggtt aagattattc ctttggaagt cgtgctccgc
     4981 aactatactg ctggttcctt ttcaaaacgt tttggtgtgg atgagggaat cgccttggag
     5041 actccgattg tcgaatttta ctacaaaaat gatgatttgg atgatccatt tatcaatgat
     5101 gagcatgtga aattcctaca gattgcgggt gaccagcaga ttgcctactt gaaggaagaa
     5161 acgcgtcgta tcaatgaact attgaaagtc tggtttactg agattgggct taaattgatt
     5221 gactttaagc tagagttcgg ttttgacaag gatggcaaga ttatcttggc agacgaattt
     5281 tcaccagata actgccgctt gtgggacgct gatggcaacc acatggataa ggatgttttc
     5341 cgtagaggat tgggagaact aaccgacgtt tatgagattg tttgggaaaa gttgcaggaa
     5401 ttgaaataat ctgtttgcaa cggaaaacct tcgtctctca actaaaagga ctcaggctga
     5461 aaaggtcccc cagacctttt cactctgtag agaactaggt gaactaacag atgtttacga
     5521 aattgtctgg gaaaagttgc agggtttaaa ataacaacct caaggctgtt tgggaatatt
     5581 gcaagagctg aaataaagga ataagaattg atggataaac gtatttttgt tgaaaaaaag
     5641 gctgattttc aggtcaagtc agagagtttg gttagagagc tccagcacaa cttgggactg
     5701 tcaagcttga aaagtattcg tattgtgcaa gtatatgatg tatttgactt ggctgaggac
     5761 ttgtttgcac ctgcagagaa gcacattttc tctgagcagg gaaccgacca tgttttagat
     5821 gaagtatctg tgcaggcgga tcttgctaac tatgctttct ttgccattga aagtctgcca
     5881 gggcagtttg accagcgtgc agcttcgtca caggaagcct tgcttttgtt gggaagttcg
     5941 agtgacgtga cagtcaacac agcccaactt tacttggtga ataaagatat tgatgcgact
     6001 gagttggaag ctgtcaaaaa ctacctgctc aatccagttg attctcgttt caaggatatc
     6061 acgacaggga ttgccaagca ggagttttca gagtcagaca agaccattcc aaaattgact
     6121 ttctttgaaa gctatacagc agaagacttt gctcgctaca aggccgagca agggatggcc
     6181 atggaagtgg atgatttgct ctttatccaa gactacttta agtcaatcgg gcgcgtgcca
     6241 actgagactg aactcaaggt tttggacact tactggtctg accactgccg tcatacgact
     6301 tttgagacag agttgaaaca catcgacttt tcagcttcta aatttcaaaa gcaattgcag
     6361 tcaacctatg acaagtatat tgccatgcgc gaggagttag gtcggtctga aaaaccacaa
     6421 accttgatgg atatggcgac tattttcggt cgttatgagc gtgctaacgg acgattggat
     6481 gatatggaag tctctgacga aatcaatgcc tgctcagttg aaattgaagt ggatgttgat
     6541 ggtgtcaagg aaccttggct cctcatgttt aaaaatgaaa cccacaacca tccaacagaa
     6601 attgagccat ttggtggagc ggctacctgt atcggtggag ctattcgtga tccgttgtca
     6661 ggccgttcct atgtttacca agccatgcgt atttcaggtg ctggtgatat tacagcaccg
     6721 atttcggaaa ctcgtgctgg gaaattgcca caacaagtca tttctaaaac agcagctcat
     6781 ggttattctt catatggtaa ccagattggg cttgccacaa cctacgttcg tgaatacttc
     6841 cacccaggct ttgtagctaa acgtatggaa cttggtgccg ttgttggtgc ggctcccaag
     6901 ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga tcatccttct cggaggcaaa
     6961 acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta aggttcaaac agttgagtct
     7021 gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca tcgaagaacg caagattcag
     7081 cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga agtccaatga ctttggggca
     7141 ggcggcgtct gtgtggctat cggtgaattg gcagacggtc ttgaaatcga cctcaacaag
     7201 gtgcctctta aataccaggg cttgaatggt acagaaattg ccatctctga atcacaagaa
     7261 cggatggcgg tcgtggttcg tcctgaagat gtggatgcct tcgttgccga atgtaacaaa
     7321 gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa aaccaaatct tgtcatgcac
     7381 tggaatggtg agacaatcgt tgacttggag cgtcgtttcc ttgacaccaa tggtgtgcgc
     7441 gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac tcccagaaga gcgtcaaaca
     7501 tctgctgaaa cactggaatc agataccctt acggttctat ctgacctcaa ccatgcaagt
     7561 caaaaaggat tacagactgt ctttgactgc tctgttggac gctcaacggt taatcaccca
     7621 cttggtggtc gttaccaact cacaccaact gaggcatctg tgcagaaatt gccagttcaa
     7681 cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt tcaacccata tgtagctgaa
     7741 tggtctccat accacggtgc tgcttacgcg gttatcgaag caactgctcg tttggtggct
     7801 gctggtgcca actggtctaa ggctcgtttc tcttaccaag aatatttcga gcggatggac
     7861 aagcaagcag agcgttttgg tcagccagta gccgcccttc taggctctat tgaagctcag
     7921 attcaacttg gcttgccatc tatcggtggt aaggactcca tgtctggtac ctttgaagaa
     7981 ttgaccgttc cgccaacctt ggttgccttt ggggtgacga cggcagatag ccgtaaggtg
     8041 ctctctccag aatttaaagc tgttggggaa aatatctact acatcccagg tcaagccctc
     8101 tctgcagaga ttgattttga cttgattaag aaaaattttg ctcagtttga agcaagccag
     8161 gctgaccata aagtgacatc tgcatcagct gtcaaatacg gtggtgtagt tgaaagtttg
     8221 gctcttgcta cctttggaaa ctatattggt gcagaggtga ccttgcctga acttaaaaca
     8281 gctttgacag ctcaattagg cggctttgtc ttcacatctc ctgaagaaat tgctggagta
     8341 gagaaggttg gacaaacgaa agcagacttt acactgactg tcaacggtgt gaagctagat
     8401 ggacacaagc ttgacagtgc atttcaaggg acattggaag aagtttaccc aacagaattt
     8461 acccaagcga aagaactaga agaagtacca gctgtggcat cagatgttgt gattaaagcc
     8521 aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct ttccaggaac caactcagaa
     8581 tatgattcag ctaaggcatt tgaaaaagaa ggcgcagagg tcaacttggt gccatttgtg
     8641 accttgaatg aagaggctat tgtcaagtca gttgaaacca tggttgacaa catcagtgaa
     8701 gctaacattc tcttctttgc aggtggcttc tcagctgcgg acgaaccaga tggttcagct
     8761 aagtttattg tcaatatcct gctcaatgaa aaagtgcgtg cagctattga tagctttatc
     8821 gctcgtggtg gcttgattat cggtatttgt aatggattcc aagccttagt caaatcgggt
     8881 ctcctaccat acggaaactt tgaagatgct aacagtacta gcccaaccct cttctacaat
     8941 gatgccaacc aacacgtggc caagatggtg gaaactcgca ttgccaatac caactcacca
     9001 tggttggctg gtgtgcaagt gggcgatatc cacgctattc ctgtttcgca cggtgaaggg
     9061 aagtttgtcg tgacggttga ggaatttgca gagctccgtg acaatggaca aattttcagc
     9121 caatacgttg actttaacgg taaaccaagt atggattcta agtacaatcc gaatggttct
     9181 gtccatgcca tcgaaggaat taccagcaag aatggtcaaa tcatcggtaa gatgggccac
     9241 tcagaacgtt atgaggacgg tcttttccaa aatatcccag gcaataaaga ccagcacctg
     9301 ttcgcatcag cggttaaaca tttcactgga aaataagact tacagatttt ctaatagata
     9361 gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat gattacaaat gaaaattagg
     9421 tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga atgtggtgtt ttcggtattt
     9481 ggggacatcc agatgctgct aagttgacct attttggact ccacagtctt caacaccgtg
     9541 gtcaggaggg ggcaggaatc ctctccaatg atcaaggaca gctgaagcgc catcgtgaca
     9601 tggggctttt atcagaagtt ttcagaaatc cagccaattt ggataaattg acaggagctg
     9661 gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc ttctgttgat aacatccaac
     9721 ccttcctctt ccgttttcac gatatgcagt ttggtttggc tcataatgga aatctgacca
     9781 atgcagcctc tctcaagaaa gaactggaac aaagaggagc aattttcagc gcgacttcgg
     9841 actctgaaat cttggctcac ctcattcgtc gcagtcataa tcctagcctg atgggcaaaa
     9901 tcaaggaagc gctcagcctt gtcaaaggtg gttttgccta tatcttgctg tttgaggaca
     9961 agttgattgc ggctcttgac cctaatggct ttcgtccgct ttctatcggg aaaatggcca
    10021 acggagcggt ggttgtttcc tctgaaacct gtgcttttga ggtcattggt gccgagtgga
    10081 tttgtgattt gaagccaggt gagattgtga tcattgatga cgagggcatt cagtatgaca
    10141 gctatacaga tgatacccag ttggcgattt gttctatgga gtatatctac tttgcccgcc
    10201 ctgattctaa tatccacggt gtcaatgtcc atacggcacg taagagaatg ggagcgcaat
    10261 tggcgcgaga atttaagcat gaggcagata ttgtagttgg tgtgcccaat tcttccctaa
    10321 gcgcggctat gggatttgcg gaagaatcag gcttaccaaa tgaaatgggt ctgatcaaaa
    10381 accaatacac ccagcgaact tttatccaac cgactcaaga attgcgggag caaggagtgc
    10441 ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa acgtgtggtc atggtggatg
    10501 attccattgt acgtggaaca acctctcgtc gtatcgttca gctcttgaaa gaagcgggtg
    10561 cgactgaggt tcacgttgcc attggaagtc ctgcactagc gtatccatgt ttctacggga
    10621 ttgatatcca gacccgtcag gagctgattg cagccaatca tacggtcgaa gaaactcgcc
    10681 aaatcattgg tgcggacagt ctgacttatc tttcaattga tagcttgatt gagtcgattg
    10741 gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc ttactttgac ggtgactacc
    10801 caacgcctct ctatgactac gaagaagact atcgtagaag tttggaagaa aagaccagtt
    10861 tttacaagta ggcgacagat tctccattaa agaaaaggaa aaaacaaatg acaaataaaa
    10921 atgcatatgc ccagtcgggt gtggatgttg aagcgggtta tgaagttgtt gaacggatta
    10981 aaaagcacgt ggcccgtacg gagcgtgcag gtgtcatggg agctcttggt ggctttggtg
    11041 gtatgtttga cctttccaag actggggtta aagaacccgt cttgatttca gggactgacg
    11101 gtgtcggaac caagctcatg ttggctatca agtacgacaa gcacgatacc atcgggcagg
    11161 actgtgtggc catgtgtgtc aacgacatca ttgctgcagg tgcggaaccc ctctattttc
    11221 tcgactacgt agcgacaggg aagaatgaac cagctaagct agaacaagtg gttgctggtg
    11281 tggcagaagg ttgtgtgcag gctggtgctg ccctcatcgg tggggaaacg gctgaaatgc
    11341 cgggcatgta cggcgaagac gactatgact tggctggttt tgcggtcggt gtggctgaaa
    11401 aatctcaaat cattgacggt tcaaaggtgg tagagggaga tgttcttctc ggacttgctt
    11461 caagtgggat tcactcaaat ggttactctt tggttcgtcg tgtctttgcg gattacacag
    11521 gtgaggaagt cctaccagaa ttggaaggca agaaacttaa ggaagttcta cttgagccga
    11581 ctcgtatcta tgtcaaggct gtcttgccgc tcatcaaaga agagttggtc aacggcattg
    11641 cccacatcac aggtggtggc tttatcgaaa atgtccctcg tatgtttgca gatgacctag
    11701 ctgctgaaat tgatgaaagc aaagttccag tgcttccaat tttcaaagcc cttgaaaaat
    11761 atggtcagat taaacacgaa gaaatgtttg aaatcttcaa tatgggtgtg ggacttatgt
    11821 tggcggtcag ccctgaaaat gtagagcgtg taaaagaatt gttggatgaa gcagtctatg
    11881 aaattggtcg catcgtcaag aaagaaaacg aaagtgtcat tatcaaatga aaaaaatagc
    11941 ggtttttgcc tctggtaatg gctcaaattt tcaggtgatt gccgaagaat ttccagtgga
    12001 gtttgtcttt tcagaccatc gtgatgccta tgtgcttgag cgtgcaaagc agctcggcgt
    12061 tctgtcctat gcttttgaac tcaaggagtt tgagagcaag acagactacg aagcagccct
    12121 tgtcgaactc ttggaagaac accagattga cttggtttgc ctagcaggct acataaaaat
    12181 cgttggacca accttattgt cggcttatga aggtcggatt gtcaacattc atccagccta
    12241 cttgccagaa tttccaggag ctcatgggat tgaggatgct tggaatgctg gcgtgggtca
    12301 gtctggtgtg accattcact gggtggattc gggtgtggat acaggccagg tcatcaaaca
    12361 ggttcgtgtg ccacgactag ctgatgatac cattgacaga tttgaagctc gcatccatga
    12421 agcagagtac aggttgtatc cggaagtagt gaaggctcta tttacagatt gactttttga
    12481 tgattcatat gatatctttg attttaaatt ggagtcagtg tttgttgaag acggcttcaa
    12541 acggaggtat ttgtaatgtt agaatctaaa aaaacaactc gatatgtatt ttatgtctat
    12601 ctgatgttat taacttgggg aatcttattt aagtttgaaa caaatcctga atttatagca
    12661 tttttcttag ctccaaggta tatcaattgg attccatttt cagaaccact aatagtcgat
    12721 ggaaaaattg tttttgctga aatgttattt aatctgattt tctttattcc attaggtgtt
    12781 tgtttccctt tgataaaaac taatttatct agtttaagaa tagtcgggac aggtttcttg
    12841 attagtttat tgtttgagtg cttacagtat attttagcaa taggtataac agatataacg
    12901 gatttgactt taaatacgct aggtgtctgt gtaggcttac tgatttatca aatttttata
    12961 agagtgttca aatcacagac tagaaaatgg atcaatatct taggtatgct tagccttggt
    13021 tttgcttatc ttgttttact gttactgcat ttaattggtg tttaactaat gattaaaaag
    13081 gagaatataa tgactaaacg cgtcttaatc agcgtctcag acaaagcggg cattgttgaa
    13141 tttgcccaag aactcaaaaa acttggttgg gagattatct caacaggtgg aactaaggtt
    13201 gcccttgata atgctggggt ggataccatt gctatcgatg atgtgactgg tttcccagaa
    13261 atgatggacg gtcgtgtgaa gaccctccac ccaaatatcc acggagggct tctcgctcgt
    13321 cgtgacttgg atagccactt ggaagcggct aaggacaaca agattgagct cattgacctt
    13381 gtggtggtca acctttaccc atttaaggaa actatcctta aaccagatgt gacttatgct
    13441 gatgcagttg aaaatatcga tattggtggg ccgtctatgc ttcgttcagc agcgaaaaat
    13501 catgccagtg ttacagttgt ggtagatcct gctgactacg ctgtggtttt ggacgaattg
    13561 gcagcaaacg gcgaaacctc ttatgaaact cgccaacgtt tagcagccaa agtatttcgt
    13621 cacacagcgg cttatgacgc cttgattgca gaatacttca cagctcaagt gggtgaaagc
    13681 aagcctgaaa aactcacttt gacttatgac ctcaagcaac caatgcgtta cggtgagaat
    13741 cctcaacaag acgcggactt ttaccagaaa gctttgccta cagattactc tattgcttca
    13801 gccaaacagc tcaacgggaa agaattgtca tttaataata tccgtgatgc agatgctgct
    13861 atccgtatca tccgtgactt caaagatagt ccaaccgttg tggctctcaa acacatgaat
    13921 ccatgtggaa ttggtcaagc tgatgacatc gagactgctt gggactacgc ttatgagtct
    13981 gacccagtat ctatctttgg tgggattgtc gtcctcaacc gtgaggtgga tgctgcgaca
    14041 gctgagaaga tgcacggcgt tttcctcgaa atcatcattg caccaagcta tacggatgaa
    14101 gcgctagcca ttttgatcaa taaaaagaaa aacttgcgta tccttgcctt gccatttaat
    14161 gctcaagagg ctagcgaagt ggaagcagaa tacacaggtg tagtcggtgg acttctcgtg
    14221 caaaatcaag acgtggtcaa ggaaagccca gctgactggc aagtggtgac caaacgtcag
    14281 ccaaccgaga cagaagcgac tgctcttgag ttcgcttgga aggctatcaa gtacgtcaaa
    14341 tcaaatggta ttatcgtgac caacgaccac atgacacttg gtgttggtcc aggtcaaacc
    14401 aaccgtgtgg cttctgttcg ccttgccatt gaccaagcca aagatcgtct gaacggggcg
    14461 gtccttgctt cagatgcctt cttcccattt gcggataacg tggaagaaat cgccaaagca
    14521 ggaattaagg ccatcatcca gcccggtggc tctgtccgtg accaagaatc catcgaagca
    14581 gcggataaat acggcttgac tatggtcttt acaggtgtga gacattttag acattaagaa
    14641 gataaaaggg aagaaaacag tttctttcct tttttggctt aaaatgctaa ctgaaacaag
    14701 attaaaacga acgtttatgt tataatgtta gtaaatgatt cgtaaaagag gttgagaaat
    14761 gaaactgctt gttgtcggtt cgggtggtcg tgagcatgcg attgctaaaa agttacttga
    14821 atcaaaagac gtggaaaaag tctttgtagc tcctgggaat gatgggatga ctctggatgg
    14881 tttggaattg gtaaatatct ctatttccga acattctaaa ttgattgact tcgcaaagac
    14941 caatgatgtt gcttggacct ttatcggtcc agatgatgcc cttgctgctg gtatcgtgga
    15001 tgattttaac caagctgggc ttaaggcctt tggtccgact agggctgcag cggagctgga
    15061 gtggtccaag gattttgcca aggaaatcat ggtcaaatat ggtgtttcga cagcagccta
    15121 tggcacattt tcagatttcg aggaagcaaa ggcctatatc gaaaagcatg gtgcgcctat
    15181 cgtagtcaag gcggatggct tggcgcttgg gaagggtgtc gtcgttgctg agacggttga
    15241 gcaagcggtc gaagccgctc atgagatgct tttggacaat aaatttggtg actcaggtgc
    15301 gcgcgtggtt attgaggaat tccttgaagg agaggaattt tcactctttg cctttgtcaa
    15361 tggtgataag ttctacatca tgccaacggc tcaggaccac aaacgtgcct acgatggcga
    15421 caaagggcct aacacgggtg gtatgggtgc ctatgcgcca gttccacact taccacagag
    15481 tgtagttgat acagcggttg acaccattgt caagccagtt ctagaaggga tgattaaaga
    15541 aggtcgccct tatttgggag ttctttacgc agggcttatc ctgacagctg atggaccgaa
    15601 agtcattgag ttcaacgctc ggttcggaga tccagaaact cagattatct tgcctcgctt
    15661 gacctctgac tttgctcaaa atatcacaga tatcctggat agcaaggagc caaatatcat
    15721 gtggacggac aagggtgtga ctctgggtgt ggttgtcgca tccaagggct acccgctaga
    15781 ctatgaaagg ggcgttgagt tgccagccaa gacagaaggc gatgtcatca cctactatgc
    15841 aggggctaag tttgcggaaa atagcagagc actgctctca aacggcggac gagtttatat
    15901 gctcgttacc acagcagata ccgtcaaaga agcccaagcc agcatctacc aagaactata
    15961 ccaacaaaaa atagaaggac tcttctaccg aacagatatc ggaagcaagg caattaagta
    16021 aagatataag aataacgcgc cgtagtcgcc aaacacgata atggtcgtcg tggtgaaaag
    16081 accagaacag tgaatgttct ggtcaggggg gaacttggag accgtaggct caaagtttag
    16141 gaatgaaacc gaaggtttgc ttacgcctcc atcacctaag accattatca aaaagaaaaa
    16201 taaaaattca caaaatacgt taatgatcgt atggtttgcg agcgttagcg agctaatata
    16261 gaacaatcac cgccgttgtg aaagaacgat tggatgataa tccaatcgtt cagggaaatt
    16321 ggaagacctt gggtttccaa tttaggcatg agacaccttt ggtggctgct gccgtccctc
    16381 acaagctaag gtgattgttg aaaaagagga aaaaggagaa gaaatgaaac cagtaatttc
    16441 catcatcatg ggctcaaaat ccgactgggc aaccatgcaa aaaacagcag aagtcctaga
    16501 ccgcttcggt gtagcctacg aaaagaaagt tgtttccgca caccgtacac cagacctcat
    16561 gttcaaacat gcagaagaag cccgtagtcg tggcatcaag atcatcatcg caggtgctgg
    16621 tggcgcagcg catttgccag gcatggtagc tgccaaaaca acccttccag tcattggtgt
    16681 gccagtcaag tctcgtgctc ttagtggagt ggattcactc tattctatcg ttcagatgcc
    16741 gggtggggtg cctgttgcga ccatggctat cggtgaagct ggagcgacta acgcagctct
    16801 ctttgccctc cgtctcctct ctgtagaaga taagtccatt gcggatgcac ttgccaactt
    16861 tgctgaagaa caaggaaaaa tcgcagagga gtcgtcaaat gagctcatct aaaacaatcg
    16921 gaattatcgg tggcggtcaa ctgggtcaga tgatggccat ttctgctatc tacatgggcc
    16981 acaaggttat cgcgctggat cctgcggcgg attgcccggc ctctcgcgtg gcggaaatca
    17041 ttgtggcacc ttataacgat gtggacgccc tccgtcagtt ggcagaccgt tgcgatgtcc
    17101 tcacttacga gtttgaaaat gtcgacgctg acggtttgga tgccgttatc aaggatggac
    17161 aactccctca aggaacagat ctgctccgca tttcgcaaaa tcgtattttt gaaaaggact
    17221 ttttgtcaaa caaggctcaa gtcactgtgg caccctacaa ggtcgtgact tctagcctag
    17281 acttggcaga tatcgacttg tcgaaaaact atgtcctcaa gactgcgact ggtggctacg
    17341 atggtcatgg acaaaaggtt attcgttcag aagtagactt ggaagcagcc tatgcgctag
    17401 cagactcagc agactgcgtc ttggaagaat ttgtcaactt tgaccttgag atttctgtca
    17461 tcgtgtcagg aaatggcaag gaggtgacgt ttttcccagt tcaggaaaat atccaccgca
    17521 acaatatcct gtctaagacc atcgtaccag cccgcatttc tgaaagtcta gtagacaagg
    17581 ctaaagctat ggcagtgcga atcgcagaac aactcaactt gtctggaact ctctgtgtgg
    17641 aaatgtttgc gactgatgat gacgttattg tcaatgaaat cgccccacga ccacataact
    17701 ctgggcacta ttctattgaa gcctgtgatt tctctcagtt tgacacccat attctcggtg
    17761 ttctcggagc accattacca gtcatcaaac tccatgcgcc agccgttatg cttaatgtcc
    17821 tcggtcagca tgtcgaggct gctgagcaat atgtgacaga aaattcaagc gcccacctcc
    17881 acatgtatgg taaaatagaa gcgaagcaca accgcaagat gggacatgtg accttgttta
    17941 gtgatgtacc ggatagtgtg gatgagcttg gggaagggat tgatttttag gacaagtcta
    18001 tgatacaaat tatcgttaat acatttattg aaaaggataa gactggagca gttgttgaag
    18061 tgttgtatgc cagtgctgac caagataagg tacaagctaa atatgaagaa ctagctgcac
    18121 aataccccga aaattattta gctatctatg atgtaccgct ggatacggat ttgaatacac
    18181 tagatcatta cccgtctgtg tttattgaaa aagaggagtt tgagtagaaa tcttggttta
    18241 cctagatagc ttattcccaa cagcttaaga agaaaggaaa aattaacaca tgatcaaccg
    18301 ttactctcgc cctgagatgg cgaatatttg gagtgaagaa aataaatacc gtgcttggct
    18361 tgaggtggaa atcctctctg acgaggcatg ggctgagttg ggggaaatcc ctaaggaaga
    18421 tgtggctttg attcgcaaga aggcggactt tgacatcgac cgtattttgg aaattgagca
    18481 ggagacgcgc cacgatgtgg tggctttcac gcgtgcggtt tctgagactc ttggtgaaga
    18541 gcgcaagtgg gttcactatg ggttaacttc taccgacgtg gtggatactg cttatggtta
    18601 cctctacaag caggccaacg acatcatccg tcgtgacctt gaaaacttca ctaatatcat
    18661 cgctgacaag gccaaggagc acaagttcac catcatgatg gggcgtactc atggtgtgca
    18721 cgctgagccg acaacctttg gtcttaaatt ggcaacttgg tacagcgaaa tgaaacgcaa
    18781 tatcgagcgt ttcgagcatg cagctgctgg tgtagaagct ggtaagattt ctggtgcggt
    18841 tgggaacttt gccaatatcc caccatttgt agaggagtat gtctgcgata aacttggcat
    18901 ccgtgcccaa gaaatctcta cacaagtcct tcctcgtgac cttcacgctg agtactttgc
    18961 ggttcttgcc agcattgcga cttcaatcga acgtatggcg actgagattc gtggtctaca
    19021 aaaatctgag caacgcgaag tagaagagtt ctttgctaaa gggcaaaaag ggtcttcagc
    19081 aatgcctcac aaacgcaacc caatcggttc tgaaaatatg actggtctgg cgcgtgtcat
    19141 tcgtggtcac atgattacgg cttatgaaaa cgtcgctctc tggcacgaac gcgatatttc
    19201 tcactcatca gctgagcgta tcatcacacc agatacgacc attttgattg actacatgct
    19261 caaccgtttt ggaaatatcg tcaagaactt gacagtcttc ccagaaaata tgatccgaaa
    19321 catgaactcg acttttggtc ttatctttag ccaacgggct atgttgacat tgattgaaaa
    19381 aggcatgacc cgtgagcaag cctatgactt ggtgcaacca aaaacagcct actcttggga
    19441 caaccaagta gactttaaac cacttcttga ggcagattca gaagtaacat cacgtctcac
    19501 tcaagaagaa atcgatgaaa tcttcaaccc agtttattac accaaacgag tggatgatat
    19561 ctttgaacgt cttggactag gtgattaatt aaaaaataaa cagcgagctt caatctcgct
    19621 gtttattttt tatcgaaaag acttagtctt cttttctttt agtgagtcca taggctgcta
    19681 gtgtggacat gagtcctgcg actactagtc ctgcagaatc gtgagttcct gtttcaggaa
    19741 gttttttctc tgttaccaca ggagctggat cttgaggaag agctttgctt tcctcagcag
    19801 gagcagttga tggagctggt tggcttggga tttctagttt tggtttttct tcagcaatag
    19861 cggcttgtcc gttttcatcg cctacatgtg ttaccatagt tccgacttcg actatttgag
    19921 taacggcttc ctgtgctacg acactattta caagtgtttt cacttcctta ccatcggcag
    19981 aagtgctcac agagtagaag ttgctacgat gtccattgac gcccttagta atgacttgtg
    20041 tttttccttt gagtaagagt ggattttcac gagtcactgt ggtaaatgga atttcttctt
    20101 tttggatatc cagtctaggt tttacctcag tagttggtgc aagaccactt tcatcaccct
    20161 tgtgagttac aggagcgcca acttcaacca cttggtttat aacttctttg gttacctggc
    20221 tatcaaggac tgtttctgtt gtttttccat tttcagtgag tacagagatg taatgagttc
    20281 gttcaccttt gactcctgct gtgataatat tttcctgacc agctgggagg ttaggatttt
    20341 ctttcttgat aacttcaaat ggaatttctt cagttcttgt gatgagttct ggtctggttt
    20401 caacattggc agccacttca ttttcatcta ggcttcctga atgagttgca gctggtttga
    20461 ggccttgaag agcggctttt aggttggcta caagtgtgtc aagctcagct tgtttattac
    20521 ggttgaggtt gtaatttaga gctgttttag ctgcgtcaag ggcctcaaga ctttctttac
    20581 tatatccttc taagtttgta ggaattttag ctaattcttc gcggagagca ttataattag
    20641 cacggaagta gtctttgttg tggtctgcaa aggcagtcat gagttcaaag atttcctctt
    20701 ccttgtactc agcgcttggt ctatctgccc agattgaaag catacttccg actgttggaa
    20761 gatctacttc aggatatttg gtagaagcta gttgattgaa tggtgttttt ccagtattct
    20821 caatagcttt cttgaggaaa ccaccaccat cttctggttt ttgaccaaga atgtagtacc
    20881 agtcaccgtt ggtattcaag aatttatagc ctttgcttgc taggtattga ggtgatgcga
    20941 ggttatatcc ccaccagcct ttagaccagt aagaaatcaa gacatctttg tcaaactgaa
    21001 catcgtcctt gtcttcatag tagaagccat cgttgaaggc cattggttga agccctcttt
    21061 ctttggccat agctgcgagg gtgttggcat attcggcaaa tttgccatag agttgatacc
    21121 acttgaggta gtaccagcct tgggcactag tcgcatcgtt ggcgtattcg tcagtaccaa
    21181 agttgaaaat ctttgtttta cctgcaaaga agtccatgta tttaccgatg agggctttta
    21241 caaagttcat cgcttcttcg ttttttaagt ccatagttgt ttttgaaact ttatcaaagt
    21301 gggcttgagg atttttaata cctaattttt ccatggcaac cagcatagca tccatgtgac
    21361 ctggactgtt aatagctggg atgagaccga tgtccttaga tttagcgtat tcaattagct
    21421 ctgttacttc tgcctgtgtt agtgcagtac cgtttggatc gtcgtagtaa gctttagttc
    21481 cttcgataat agctttttta acgtcatcac tagcataggt ttttccgttg gcagtaatgg
    21541 tcatatcatc gagtagaaag cgaagtccgt catttcctag aaggagatgg acatcagaat
    21601 atccgagctc actggccttg tctacgatgc gtttgagctg gttcagagta aagtatttgc
    21661 gtccagcatc gattgagatt accttgtttt tggcaagttt ttcaacctca cgtttagctt
    21721 cttcttcttt tagagcttca ggcgtgaggg tcaagttgtt gacagtttct tgaagtttag
    21781 caatggcttg atcaatcgta tcttgttggg cacggctaag gttgctatcg agagagcgaa
    21841 tagctttttc agcttctttt acggccgcga cgctttctgc agtataacgg ttcaggtctt
    21901 ttggtacctc gttaagtgtt tgctctgcag attcataatc agctgcgaag tattcagcgt
    21961 tggcatttgc aaaatgacgc atgagtttga agaggcgtga tggtgaataa cgtgcagatg
    22021 gagtgtcagc ccaagcagct accataccac cgatgattgg gatatcagct ccttctgttt
    22081 ttggtacaga agtgattggt gtgtttttaa taccattgag cccctgatcg agattgtacc
    22141 agccttggcc atcagcgttt cgtccaagaa cgtagtacca agcatcattg gtattaagga
    22201 tttggtgacc tttttcagct agtagtttag aagaagcgac atcgtagcct ccccaaccac
    22261 cagtccacat agaaacgatg atgtctttgt caaaactacc aaagcttgtg tcgctattgt
    22321 agtagatacc gtcgttaaaa gccattggtt tgagaccgtg cgattttaca atacgagcga
    22381 ggtcattggc gtaggcaata aatttttcat agccttttac agggtagcct tcgtttggat
    22441 agtatttatc agcttgaagc acactccaac ctttagcatc tgtcgcatca ttggcatatt
    22501 catcaagtcc gatgttgaag atttcagtct ttttcgcgaa ataagcagca tacttgtcga
    22561 taagggcttt tgtaaaagcg acagcttgtt cgttgtcaag atcgacagta cgggctgatt
    22621 tcttcccaaa atagctaaag ttagggtttt ggattcccaa ttctttcatg gcattgagaa
    22681 tcgcatccat gtgtccagga ctatttactg tcggaatgag accgatacct ttatctttgg
    22741 catagttaat cagatctgtc atttgacttt ctgttaagtg attgccgttt ggatcgttgt
    22801 aataatcatt tgtacctttt tcaatggcgc gtttgacatc gtcactggca taggtcttgc
    22861 cgttagctgt gatgctcata tcgtccaaca tgaaacggag tccatcattt ccgactaata
    22921 ggtgtaaatc agtgtagcca taatgtttcg ctttatcgat gatttccttg agttgttctg
    22981 gtgagaaata tttacgtcca gcatcaatag aaacaatttt ctttttcgct agtttttcat
    23041 ttacagttgc agcacgttcc tttcctgcct ctgtttccgg tttgtcagcc tctgctttcg
    23101 cttcatcttt tttagctggt ttatccttgt cagtcttgtc tgtatttgac tctttagaat
    23161 caacctcttt cgcttcttcc tttttaggac tagcttcttc tgccttttta ttagcagttt
    23221 ctttttcagc agaagttgga gttaccactt ctgctttatc actaggagtt gaactaactt
    23281 cctcttgtgg tttttcttct gtttttggaa gactagctac cttatcagta gctggagttt
    23341 ctgtttctac agtttttgga gcttctggtt gaagcactgc tttaggtgtt tcctcagtcc
    23401 gattttcgga tgattgaggg gaatcagaaa ccgtatggat ggtcggttgg ttttctgtag
    23461 tagtaggagt aactccatcg gctgcaacag tctgtgcttg gaaggcaaat ccaattagaa
    23521 cagaagctgc tcctacagcg tatttacgaa tagaaaaacg ctgttgtttt tcatgtttca
    23581 ttgcaaaacc tcctgattgc attgttatat tgatagcgat tatataaatc aacgccttta
    23641 ttttatttct tatattaacg agagtcaaga ggagatgaca aaaaactata ataagtataa
    23701 aaaaatataa aatttaaact taagatttca gattggtcgg aaaaaatgaa tatatatatc
    23761 tagtataatt tttggttcta tttctataaa atattccaca aattatagaa tcttccaaaa
    23821 ataggtaagc gctacctttt tggtgtggta taataagcat agaaaaagcc caagcgatta
    23881 gctcaggctt tcttcttagt gatcacggtc acatgagata aatttaatct tgtagtaatc
    23941 agatcgtttg taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat
    24001 tttagtttgt aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc
    24061 tggagttgga aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg
    24121 gtagtctaac ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc
    24181 gttgatatat tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc
    24241 gcggatacgt tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaagta
    24301 aacaagcttg tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa
    24361 gagttcaata tctgaaaact ctacaagctt gtgtttgcgc gcacgtgaaa cgaaggttcc
    24421 ttttccttgt tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac
    24481 tgtgatagag ctgacatcgt acattgaaat gagttctgct tcagtgtaaa atttatctcc
    24541 actgctaaac tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt
    24601 gggaattgcc atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc
    24661 gaaaaaaata gtagtctttt gaataaaaaa ttagaataat agtttgaaaa attaagcgat
    24721 atagggattg ctcatatttt ataaatgtat tctatacaaa attattttag gtcctatatt
    24781 ccgaatttaa agagcgtttg aaatgggtct aaaaatgttt gaaactaatt gaatgtgtgt
    24841 ataatagtaa aaatgcagta aatatcccga caatttctat tgacaattca aacagattgg
    24901 tttataatta atataacaac aagtgaaagc gcaaactttc gcggtcggaa ggtagtttta
    24961 tgacacgatt tgagatacga gatgatttct atctcgatgg aaaatcattt aagattttat
    25021 ctggtgccat tcattatttt agggttcctc cagaggattg gtatcattcg ctctataact
    25081 tgaaggctct tggttttaat acggtagaga cttatgttgc ttggaattta cacgagcctc
    25141 gtgaaggtga gtttcatttt gaaggtgatc tggatttaga gaaatttctc caaatagcgc
    25201 aggatttggg tctctacgca attgtgcgtc cgtctccatt tatctgtgcg gaatgggaat
    25261 tcggtggctt accagcttgg ctcttgacca agaacatgcg aattcgctca tccgacccaa
    25321 catatatcga ggcagttggt cgctactatg atcagttatt gccaagactg gttcctcgtt
    25381 tgttggacaa tggtggcaat attctcatga tgcaggttga aaatgagtat ggttcttacg
    25441 gagaagataa ggcttacctg agagcgattc gacagctaat ggaagagtgt ggcgtaacct
    25501 gtcccctctt tacatcagat ggtccatggc gagctactct gaaagctgga accttaattg
    25561 aagaggacct ctttgtaaca ggaaactttg gttctaaggc accttacaac ttttcgcaga
    25621 tgcaggaatt ctttgatgaa catggtaaga aatggccact catgtgtatg gagttctggg
    25681 atggttggtt taatcgctgg aaagaaccga ttatcacacg ggatcctaag gaattggcag
    25741 atgcagttcg agaggttttg gaacaaggct ctatcaatct ttacatgttc cacggtggta
    25801 caaactttgg tttcatgaat ggttgctcag ctcgaggaac tttggacctg ccacaagtta
    25861 cgtcttatga ttacgatgcc cttctggatg aagaaggaaa tccaactgct aaatatcttg
    25921 cagtcaagaa gatgatggca acacattttt cagagtatcc gcagttggaa ccactctaca
    25981 aagagagtat ggagttggat gctattccac tagttgaaaa agtttctttg tttgaaacct
    26041 tagatagctt gtcaagtcct gtagaaagtc tctatcctca aaagatggag gagctgggac
    26101 aaagttatgg ctacctactt tatcgaacag aaacaaactg ggatgcagaa gaagaaagac
    26161 ttcgtatcat tgatggtcga gatagggccc agctgtatgt cgatggtcag tgggttaaaa
    26221 ctcaatatca gacagagatt ggggaagata ttttttatca aggtaaaaag aaagggctat
    26281 ctaggttaga tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat aagttcttag
    26341 cggatacgca acgtaaggga attcggacag gggtctgtaa ggatctgcat ttcttactaa
    26401 actggaaaca ctatccactc ccactagaca atcctgagaa aattgatttt tcaaaaggat
    26461 ggactcaagg acaaccagcc ttttacgctt atgactttac agtcgaagag ccaaaagata
    26521 cttacctaga cttgtctgag tttggtaagg gggttgcctt tgtcaatggg cagaatctag
    26581 gacgtttttg gaacgttggc ccaactctct cactttatat ccctcatagc tatctcaagg
    26641 aaggtgccaa ccgcatcatt atctttgaaa cagaaggtca atataaagaa gagattcatt
    26701 taactcgtaa acctacacta aaacatataa agggggaaaa cttatgacaa ttgtaggatg
    26761 ccgtattgat ggacgtttga tccacggaca agtagccaat ctttgggctg gaaaactaaa
    26821 tgtttcacgc attatggttg tagacgacga agttgtcaac aacgatattg aaaagagtgg
    26881 tttgaaactt gcgacaccac caggtgtgaa attgagtatt ttgccagttg agaaagctgc
    26941 agccaatatt cttgctggca aatacgatag ccaacgtctc tttatcgtgg ctcgtaaacc
    27001 agaccgcttc cttggtttgg tagaagcagg tgtaccactt gaaaccctta atgttgggaa
    27061 tatgtctcaa acaccagaaa ctcgttctat tacacgttct atcaacgtag tagacaagga
    27121 tgtggaagac ttccacaaac tggcagaaaa aggtgttaaa cttactgctc agatggttcc
    27181 aaatgatcca atttcagact ttttgagctt attaaaatag gaaaaaaatt tttaggaggt
    27241 cattgttatg atacaatggt ggcaaatttt acttctcact ttgtactcag cttatcaaat
    27301 ctgtgatgag ttgacgatcg tttcatctgc aggttcccct gtatttgctg gtttcattac
    27361 tggtttaatc atgggagatg tgactactgg tttacttatc ggtggtaact tgcaactgtt
    27421 cgttcttggg gttggtacct tcggtggtgc ttctcgtatc gacgcaactt ctggtgcggt
    27481 tcttgcgaca gccttctctg tttcacaagg aattgatgca ccgcttgcaa ttactacaat
    27541 cgctgtacca gtagcagctc tcttgactta cttcgacgtt cttggtcgta tgactactac
    27601 cttcttcgct caccgtgtgg atgctgcaat cgaacgcttt gactataaag gtattgaacg
    27661 caactacttg cttggtgcga ttccgtgggc tctatctcgt gcccttccag tcttctttgc
    27721 ccttgctttt ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag cctacaaatg
    27781 ggttgcagat ggcttgacac tcgcaggacg tatgcttcca ggtcttggat ttgcaatctt
    27841 gcttcgttac cttccagtta aacgtaacct tcactacctt gctatgggat ttggtttgac
    27901 agctatgttg actgttcttt actcatatgt aacaggtctt ggtggcgctg ttgctggtat
    27961 cgtaggtact cttcctgctg aagttgctga aaaaattggt ttcgtgaaca acttcaaagg
    28021 tttgtctatg attggtattt ctatcgtagg tattttcctt gcagtgcttc acttcaaaaa
    28081 tagccaaaaa gtagctgtag cagcaccttc tacaccatca gaaagtgggg aaatcgaaga
    28141 tgacgaattc taattacaaa cttacaaaag aagattttaa tcaaatcaac aaacgtagct
    28201 tgtttacttt ccaattaggt tggaactacg aacgtatgca agcttctggt tacctttaca
    28261 tgatcttgcc tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg aaagaaatga
    28321 tgaaagttca tactcaattc ttcaatactt caccattctt ccataccatt atcgctggtt
    28381 ttgaccttgc catggaagaa aaagatggtg taggttcaaa agacgccgtt aacggtatca
    28441 agacaggttt gatgggacca ttcgctcctc ttggggatac aatctttggt tcacttgtac
    28501 ctgctatcat ggggtcagtc gcagcaacta tggctatcgc tggccaacct tgggggatct
    28561 tcctttggat tgcagttgca gtagcgtatg acatcttccg ttggaaacag ttggaatttg
    28621 cttacaaaga aggggttaac cttatcaaca acatgcaaag taccttgaca gctttgattg
    28681 acgctgcatc tgtacttggt gtcttcatga tgggtgctct tgtagcaaca gtgattaact
    28741 ttgaaatttc ttacaagttg ccaatcggtg aaaagatgat tgatttccaa gacatcttga
    28801 atcaaatctt cccacgtttg cttccagcaa tctttactgc ctttatcttc tggttgcttg
    28861 gtaagaaagg tatgaactct actaaagcta tcggtattat tatcgtactt gctttggctc
    28921 tttctgccct tggtcacttt gcacttggaa tgtaattcct tatgactaaa tcattaattt
    28981 tggtgagcca tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg attatgggcc
    29041 cacaagacaa catttacaca gtagctcttc ttccagaaga tggcccagaa gaatttactg
    29101 ctaaatttga agctgttatt gaaggattgg atgatttcct agtctttgcg gatcttctcg
    29161 gtgggacacc ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat attgaccttt
    29221 acgcagggat gaatcttcca atggtgattg aatttatcaa tgcgagcctt acaggcgcag
    29281 atgcggacta caagagccgt gctgcagaaa gcattgtgaa agttaatgac ctgttagcgg
    29341 gcttcgatga tgacgaagat gaataatact cttcgaaaat ctcttcaaac tacgtcaacg
    29401 tcgccttgcc gtaggtatat gttactgact tcgtcagtct tatccggcaa cctcaaagca
    29461 gtgctttgag cagcctgcgg ctagtttcct acagatttta gttggaactc gattcaattc
    29521 atgtgacaac gtgaaaatcg ttagagcatt ttatatagaa tatacatggg aatgtagctt
    29581 actcccattc ccatatttaa tagaaaaaga ggaactcaat gctacattat acaaaagaag
    29641 acttgctcga attgggtgca gaaatcacta cgcgtgaaat ctaccaacag cctgatgtat
    29701 ggagagaagc ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc ttcctacaag
    29761 aaatcgctga taaacatgac tatattaagg ttatcttgac aggtgctggg acttctgctt
    29821 atgtgggaga taccttgcta ccttatttta aggaagtcta tgacgaacgc aaatggaatt
    29881 tcaatgctat tgcgacaaca gatatcgttg ccaatccagc aacctatttg aaaaaagatg
    29941 tggcaactgt ccttgtgtct tttgctcgta gtgggaattc gcctgaaagt ttggcgactg
    30001 ttgatttggc caaatccttg gtggatgagc tttatcaagt gacgattact tgtgcagcag
    30061 atggtaaatt ggctcttcaa gctcacggtg atgatcgtaa tctcttgctc ttgcaaccag
    30121 ctgtctctaa tgatgctgga tttgccatga cttctagctt tacgtctatg atgttgacaa
    30181 ctctcttggt ctttgatcct acagaatttg ctgttaagtc tgaacgtttt gaagttgtat
    30241 ctagtcttgc ccgtaaagtt ttagacaagg cagaagatgt caaagagctc gttgatttag
    30301 actttaaccg tgtcatctat ctaggcgctg gtcctttctt tggacttgct catgaagctc
    30361 agctcaagat tttggaatta actgctggtc aagttgcgac catgtatgaa agcccagttg
    30421 gcttccgtca cggtccaaaa tctcttatca acgacaatac agttgttttg gtctttggta
    30481 caacgacaga ctacactcgt aagtacgact tggacttggt tcgtgaagtt gctggtgacc
    30541 agattgctcg tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa aatgtcaaag
    30601 aagtggccct tggttgtggc ggtgtcttga atgatattta ccgtgtcttc ccttacatcg
    30661 tttatgccca actctttgct ttattgactt cactcaaggt agaaaataaa ccagatacac
    30721 cgtctcctac aggtacagta aaccgtgtag tacaaggtgt cataattcac gaatatcaaa
    30781 agtaagacag tgtttatgaa ttcttgacaa gaggatttgt aaattatcag ataaaccata
    30841 gattgtcagt acgctttcta tggtttgttt gcttgagaga aatagtaaaa ggagaacaga
    30901 atgaaagcat acacagagcg tgtatttgga aatgttgagg gtgaggatgt cttggcctat
    30961 cgatttgaga cagacggtgg ctaccaactt gaggttatga cttatggtgc gactatcttg
    31021 cgctatgtcg cacctgacaa ggctggaaat tttgccaatg ttatcttggg atttgatgac
    31081 tttgatagtt atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc tgtagcgggt
    31141 cgtattgcag gtgcgacctt tgagctcaat ggtaagacct atgaccttga ggttaataat
    31201 gctagcaact gtaatcacag tggttcaact ggttgggatt ccagcttgtt tgaagttgaa
    31261 gaagtaagcg atcatggctt gactctctac acagagcgta cagatgggac aggagggttc
    31321 cctggaaatc tcaagatttg gatcagttat cacttggaag aaactggtgc ctatgaaatc
    31381 agctacaagg taacgaccga tcaggatacg ctggtcaatc caaccaacca cagctatttc
    31441 aacttgtctg gtgatttcac gcagacgatt gaccgtcatg tcttccaact aaacacagag
    31501 ggcatttact caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc caaccgtgat
    31561 gtggtcaaac acgtctacaa tggtaccttg ttgaaggata tctttgcaga agaagatgag
    31621 caaatccagc tggcatcagg tttggatcat ccatttgccc ttcctgcagg ccatgacaat
    31681 gctggattcc tttatgacca aaattcaggt cgcttcctgc ttttcaagac agaagctcct
    31741 tgctttgtgg tctacacagc aaactttgtg gatgaaagtg tcatcatagg aggtcagcca
    31801 atgctacagc acaatgggat tgctcttgaa gcgcaagctt taccagatgc cattcacagt
    31861 gaccttaaag gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa gacacgttat
    31921 gaacttgttg tgaaataaaa aaagctggtc aaaaagtctt taaaattaaa ataacgcata
    31981 ttgtcaggtg atgaaaagtt tgataatatg cgttttatta tgaaatgatt tactttatat
    32041 tatcctgaaa tggtgttatt tttgagttat ctggcttgac tttcttgatg gaaattatat
    32101 aatagttgta acaataataa ttacaactag aaaagaggac catttatgac ctatgaatac
    32161 aagagtcaca tttatttggc agagacagct ttaaatgtaa aggatttggc cagtcaaaca
    32221 gccttttatc agcaagttat tggtttagaa atattatctc aaactgagac agagtccatt
    32281 ctaggccttg gtggaaaagt cttggtacag ctgattcaag cacaagagag cggagaagtg
    32341 agggaatatt atggtcttta ccatttggcc attctcttgc ccacacgcaa ggctttagct
    32401 gatgtcttga aacgcctgac agatttacaa attcctcttg tcggcggtgc agaccatggt
    32461 tacagtgaag ctatttactt ggaagatttg gagggaaatg gcattgaact ctatcgagat
    32521 aagccagttt ccacatggga tattcgagaa gatggacgca ttatcggagt gacggaggct
    32581 cttgcggcgc aggacattta tgagttgggg gaaagagtag agccctttat cctggcagaa
    32641 ggtacaagaa tggggcatat tcatctttcc gtcaaggata gtcgaaagtc cagccagttt
    32701 tatcaaaagg tgttagggct agagaataaa ttcagtgtgc ctggcgctag ttggattgcg
    32761 gctggagatt accatcatca tttagcagtt aacgaatggg gaggaaaagg tctggcttcg
    32821 cgtaagcaag gcttaccagg tttagcctac tatgtcatcg aagtcgcaca taaaaaagaa
    32881 ctgttaacga ttgcccaacg agcacaagaa gttgacgcac caatcaaatg gatgacatcg
    32941 atccaattgg aaatcacaga ctcagatggc atcgtgaccc gtattcgttt agcaaggtaa
    33001 aattaggaat ttttaataag ttataagtat tgctagtttg ctttttgaac ggtctttggt
    33061 aacagaactg cgtgagaatt ttgacaaaga agctgattag agatataatg aagaaaaatc
    33121 gagcaaggaa aaagaactga caaattcaaa gtatattact tgtttgaaac gttcagaggg
    33181 ccagttgtgt gggattcaaa agatgattga aggagatcgt gactgtgctg atattgtgac
    33241 gcaactgaca gcagtgaaat ctagtgtaga gcgcgtgatt gagatgataa ttaccgaaaa
    33301 tcttactgaa tgtatcaatc agcccctaga tgattctgaa gctcaaaagg aacgcctaga
    33361 aaaggctatc cgatacttga ttaaacggaa ataaatgaat gttttgagac gatatgtgca
    33421 aaggtctatt aactattaga atagtttaat gtgagtcaaa agaagttgta aaaaatcttt
    33481 aaaattaaaa aaacgcatag tatcaggtgt tgaatgtact gacccccaaa agttagattt
    33541 tttctgtcta acttttgggg tgcagttcat aagaaccttg gtaatatgcg ttttattatg
    33601 tagagactta tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtagat
    33661 atggttactg acttcgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttc
    33721 gtcagttcca tccacaacct taaaacagtg ttttgagcag cctgcggcta acttcctagt
    33781 ttgcactttg attttcattg agtattactt catcttttcc tgaaattgag tttttgccca
    33841 actcctcttg attattctac ttgaccgggc caagcattca taccaccttc tacgttggta
    33901 acggtgaggc cttgggagct gagaaactgg caggtagatg cagagcggac tcctccttga
    33961 caaatgacat agtattcatg gtcaggtttg agttctttgt agccttgctc taaggtactt
    34021 aatggaagat ttttggcacc tggtgcatgt cctgcttgga attcatgtgc ttcacggaca
    34081 tcgataagtt ctagattttc attttggtat tttccataaa agtcagccat gctgatacta
    34141 gtttccatat tctactcctt ctgggttaac cattttgtat agtgaataag cgccgtcaag
    34201 gttttgggcg gtaaatcctg cttgcttgag gataggctct gcgatatagc tgcgcaaacc
    34261 actgtggcag ctgacgatgt aggcttgttt tttgtccagt tcatcagttt cctccttgac
    34321 actttgtcag tttttgatac aatagtacaa aattagagga ggcaggctat gattcagaaa
    34381 catgcgattc ctattttaga gtttgatgac aatcctcagg cggttatcat gcccaatcac
    34441 gaggggctgg acttgcagtt gccaaagaag tgtgtttatg catttttagg tgaggagatt
    34501 gaccgctatg caagggaagt aggggcggat tgtgtcggcg aattcgtttc tgctaccaag
    34561 acctatccag tctatgtcat caactacaag ggtgaggagg tctgtctggc tcaggctcct
    34621 gttggctccg ctccagcagc ccagtttatg gattggttga ttggctatgg tgtggagcag
    34681 attatctcta ctgggacctg tggtgtccta gctgatatag aggaaaatgc ctttctagtc
    34741 cctgttcgcg ctttgcgaga tgagggagcc agttaccact atgtggcacc ttgtcgttat
    34801 atggaaatgc agccagaggc tattgctgct attgaggaag ttttggaaga cagagggatt
    34861 ccttatgaag aagtcatgac ctggacgaca gacggttttt accgagaaac ggctgaaaag
    34921 gtggcttatc gtaaggaaga aggctgtgct gttgtggaga tggagtgttc tgctcttgcg
    34981 gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc agattctcta
    35041 gcggacttgg accagtacga cagtcgtgac tggggctcgg aagcttttaa taaggcgcta
    35101 gaactgagtt tagcaagtgt tcaccacctt tagttgtact ggcaaaggat ttgttttatc
    35161 ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc ttcctcttgt
    35221 cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc atatcaggtg
    35281 atgaaaactt tgatactatg cgttttatgt tggtaagatt tagagtgaga tgaaatgata
    35341 ctcttcgaaa atcttttcaa accaggtcag cttcaccttg ccgtatagat gttactgact
    35401 tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttctatcc
    35461 acaacctcaa aacagtgttt tgagcaacct gtgactagct ttctaagcga tgccttggtt
    35521 ctcattgcct ataatcaaaa agagaaattt tctcctgaaa agcatataga gtagctggcg
    35581 ttaaaagctc ctgtcttgct tttttgacct atagtcacat ctatcaagta ttgttcttgc
    35641 ctaagctatc aataaaaagg tggcattttt taggcttggt gttagtgaat tttgccttat
    35701 cctatctaag tcatttcgag ctttttatgg tacaatggaa acatgttatt caaattatct
    35761 aaggaaaaaa tagagctagg cttatctcgt ttatcgccag cccgtcgtat ttttttgagt
    35821 tttgccttgg tcattttact aggatctctt cttttgagct tgccctttgt ccaagttgaa
    35881 agctcacgag cgacttattt tgatcatctt ttcactgctg tctctgcagt ctgtgtgaca
    35941 ggtctctcaa cccttccagt agctcacacc tataatatat ggggccaaat aatctgtttg
    36001 ctcttgattc agatcggtgg tctagggctc atgaccttta ttggggtttt ctatatccag
    36061 agcaagcaaa agcttagtct tcgtagccgt gcaactattc aggatagttt tagttatgga
    36121 gaaactcgat ctttgagaaa gtttgtctat tctatttttc tcacgacctt tttggttgag
    36181 agcttgggag ctattttgct tagttttcgc cttattcctc aacttggctg gggacgtggt
    36241 ctttttagtt ccatttttct agcgatctca gccttctgta atgccggttt tgataattta
    36301 gggagcacca gtttatttgc ttttcagacc gatttactgg tcaatctggt gattgcaggc
    36361 ttgattatta caggcggcct tggttttatg gtctggtttg atttggctgg tcatgtagga
    36421 agaaagaaaa aaggacgtct gcactttcat acgaagcttg tactattgtt gactataggt
    36481 ttgttgttat ttggaacggc aactactctc tttcttgagt ggaacaatgc tggaacgatt
    36541 ggcaatctcc ctgttgccga taaggtttta gttagctttt ttcaaacagt gacgatgcga
    36601 acagctggct tttctacgat agattatact caggctcatc ctgtgactct tttgatttat
    36661 atcttacaga tgtttctagg tggggcacct ggaggaacag ctgggggact caagattacg
    36721 acattttttg tcctcttggt ctttgcacga agtgagcttc taggcttgcc tcatgccaat
    36781 gttgcgagac gaacgattgc gccgcgaacg gttcaaaaat cctttagtgt ctttattatc
    36841 tttttgatga gcttcttgat aggattgatt ctgctaggga taacagccaa aggcaatcct
    36901 ccctttatcc acctcatatt tgaaaccatt tcagctctta gtacagttgg tgtaacggca
    36961 aatctgactc ctgaccttgg gaaattggct ctcagtgtta tcatgccact tatgtttatg
    37021 ggacgaattg gtcccttgac cttgtttgtt agcttggcag attaccatcc agaaaagaaa
    37081 gatatgattc actatatgaa agcagatatt agtattggtt aagaaaggaa agagcatgtc
    37141 agatcgtacg attggaattt tgggcttggg aatttttggg agcagtgtcc tagctgccct
    37201 agccaagcag gatatgaata ttatcgctat tgatgaccac gcagagcgca tcaatcagtt
    37261 tgagccagtt ttggcgcgtg gagtgattgg tgacatcaca gatgaagaat tattgagatc
    37321 agcagggatt gatacctgcg ataccgttgt agtcgcgaca ggtgaaaatc tggagtcgag
    37381 tgtgcttgcg gttatgcact gtaagagttt gggggtaccg actgttattg ctaaggtcaa
    37441 aagtcagacc gctaagaaag tgctagaaaa gattggagct gactcggtta tctcgccaga
    37501 gtacgaaatg gggcagtctc tagcacagac cattcttttc cataatagtg ttgatgtctt
    37561 tcagttggat aaaaatgtgt ctatcgtgga gatgaaaatt cctcagtctt gggcaggtca
    37621 aagtctgagt aaattagacc tccgtggcaa gtacaatctg aatattttgg gtttccgaga
    37681 gcaggaaaat tccccattgg atgttgaatt tggaccagat gacctcttga aagcagatac
    37741 ctatattttg gcagtcatca acaaccagta tttggatacc ctagtagcat tgaattcgta
    37801 aagagggatg acccctcttt tttgatgccc aagatggcaa atagagacag aagccccttg
    37861 tcttctagta aaagttcttc aaaggctgga ctttatggta aaatagaagg aagtgacaag
    37921 agagagtaat actcaatgaa aatcaaagat caaactagga aactagctgc gggctgctca
    37981 aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaacatcta tacggcaagg
    38041 cgacgttgac gtggtttgaa gagatttttg aagagtataa gaaaaaatca gtcccctaaa
    38101 ggagtagatt atgaagttat tgtctatcgc cattcccagc tataatgcag cagcctatct
    38161 tcattactgt gtggagtcgc tagtgattgg tggtgagcaa gttgggattt tgattatcaa
    38221 tgacgggtct caggatcaga ctcaggaaat cgctgagtgt ttagctagca agtatcctaa
    38281 tatcgttaga gccatctatc aggaaaataa atgccatggc ggtgcggtca atcgtggctt
    38341 ggcagaggct tctgggcgct attttaaagt agttgacagt gatgactggg tggatcctcg
    38401 tgcctacttg aaaattcttg aaaccttgca ggaacttgag agcaaaggtc aagaggtgga
    38461 tgtctttgtg accaattttg tctatgaaaa ggaagggcag tctcgtaaga agagtatgag
    38521 ttacgattca gtcttgcctg ttcggcagat ttttggctgg gaccaggtcg gaaatttctc
    38581 caaaggccag tataccatga tgcactcgct gatttatcgg acagatttgt tgcgtgctag
    38641 ccagttctaa ctgcctgaac atacttttta tgtcgataat ctctttgtct ttacgcccct
    38701 tcagcaggtc aagaccatgt actatctgcc tgtcgatttc tatcgttatt tgattgggcg
    38761 tgaggaccag tctgtcaatg agcaagtgat gattaagtgc attgaccagc aactcaaggt
    38821 caatcgactc ttggtagacc aacttgattt gtcccaagtg agtcatccca aaatgcgaga
    38881 atatctgctg aatcatattg aaatcacgac ggtgatttcc agtaccctgc tcaaccgatc
    38941 tggaacaacg gagcatctgg caaaaaaacg ccaattgtgg acctatattc agcagaaaaa
    39001 tccagaagtc tttcaggcta ttcgtaagac catgttgagc cgtttgacca aacattctgt
    39061 cttgccagat cgcaaactgt ccaatgtcgt ctatcaaatc accaaatctg tttatggatt
    39121 taattaatat aagtgtttta taagagggat ttaagaaaaa ttttaacttt ttcttagtcc
    39181 tttttaattt caggagatta tactagagtc atcaaataaa gaaagactct aaggagaatc
    39241 ctatgaaatt caatccaaat caaagatata ctcgttggtc tattcgccgt ctcagtgtcg
    39301 gtgttgcctc agttgttgtg gctagtggct tctttgtcct agttggtcag ccaagttctg
    39361 tacgtgccga tgtggtcaat ccgaccccag gtcaagtctt acctgaagag acatcgggaa
    39421 cgaaagaggg tgacttatca gaaaaaccag gagacaccgt tctcactcaa gcgaaacctg
    39481 agggcgttac tggaaatacg aattcacttc cgacacctac agaaagaact gaagtgagcg
    39541 aggaaacaaa ctcttctagt ctggatacac tttttgaaaa agatgaagaa gctcaaaaaa
    39601 atccagagct aacagatgtc ttaaaagaaa ctgtagatac agctgatgtg gatgggacac
    39661 aagcaagtcc agcagaaact actcctgaac aagtaaaagg tggagtgaaa gaaaatacaa
    39721 aagacagcat cgatgttcct gctgcttatc ttgaaaaagc tgaagggaaa ggtcctttca
    39781 ctgccggtgt aaaccaagta attccttatg aactattcgc tggtgatggt atgttaactc
    39841 gtctattact aaaagcttcg gataatgctc cttggtctga caatggtact gctaaaaatc
    39901 ctgctttacc tcctcttgaa ggattaacaa aagggaaata cttctatgaa gtagacttaa
    39961 atggcaatac tgttggtaaa caaggtcaag ctttaattga tcaacttcgc gctaatggta
    40021 ctcaaactta taaagctact gttaaagttt acggaaataa agacggtaaa gctgacttga
    40081 ctaatctagt tgctactaaa aatgtagaca tcaacatcaa tggattagtt gctaaagaaa
    40141 ctgtc
//
LOCUS       DAVMAF010000010        37694 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMAF010000010 DAVMAF010000000
VERSION     DAVMAF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.010"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            167..823
                     /locus_tag="VYO97_000498"
     CDS             167..823
                     /locus_tag="VYO97_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1753357.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            813..1022
                     /locus_tag="VYO97_000499"
     CDS             813..1022
                     /locus_tag="VYO97_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1753358.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIK"
     gene            1240..2070
                     /locus_tag="VYO97_000500"
     CDS             1240..2070
                     /locus_tag="VYO97_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753359.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2224..3078
                     /locus_tag="VYO97_000501"
     CDS             2224..3078
                     /locus_tag="VYO97_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1753360.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3178..4551
                     /locus_tag="VYO97_000502"
     CDS             3178..4551
                     /locus_tag="VYO97_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1753361.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4544..5605
                     /locus_tag="VYO97_000503"
     CDS             4544..5605
                     /locus_tag="VYO97_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1753362.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5607..6299
                     /locus_tag="VYO97_000504"
     CDS             5607..6299
                     /locus_tag="VYO97_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753363.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6329..6898)
                     /locus_tag="VYO97_000505"
     CDS             complement(6329..6898)
                     /locus_tag="VYO97_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1753364.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVTPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7033..7647
                     /locus_tag="VYO97_000506"
     CDS             7033..7647
                     /locus_tag="VYO97_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1753365.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7649..9295
                     /locus_tag="VYO97_000507"
     CDS             7649..9295
                     /locus_tag="VYO97_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1753366.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9307..10593
                     /locus_tag="VYO97_000508"
     CDS             9307..10593
                     /locus_tag="VYO97_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753367.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10645..11178
                     /locus_tag="VYO97_000509"
     CDS             10645..11178
                     /locus_tag="VYO97_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753368.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11256..11726
                     /gene="nrdI"
                     /locus_tag="VYO97_000510"
     CDS             11256..11726
                     /gene="nrdI"
                     /locus_tag="VYO97_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1753369.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12019..13281)
                     /locus_tag="VYO97_000511"
     CDS             complement(12019..13281)
                     /locus_tag="VYO97_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1753370.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13591..14049)
                     /locus_tag="VYO97_000512"
     CDS             complement(13591..14049)
                     /locus_tag="VYO97_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1753371.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14046..14486)
                     /locus_tag="VYO97_000513"
     CDS             complement(14046..14486)
                     /locus_tag="VYO97_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753372.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14842..16791
                     /gene="mutL"
                     /locus_tag="VYO97_000514"
     CDS             14842..16791
                     /gene="mutL"
                     /locus_tag="VYO97_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1753373.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMALVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17117..17584)
                     /locus_tag="VYO97_000515"
     CDS             complement(17117..17584)
                     /locus_tag="VYO97_000515"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1753374.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17585..18790)
                     /locus_tag="VYO97_000516"
     CDS             complement(17585..18790)
                     /locus_tag="VYO97_000516"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1753375.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDILGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYNSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18810..19445)
                     /gene="ribE"
                     /locus_tag="VYO97_000517"
     CDS             complement(18810..19445)
                     /gene="ribE"
                     /locus_tag="VYO97_000517"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1753376.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19430..20530)
                     /gene="ribD"
                     /locus_tag="VYO97_000518"
     CDS             complement(19430..20530)
                     /gene="ribD"
                     /locus_tag="VYO97_000518"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1753377.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAIIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYTFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20664..20778)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            20935..21528
                     /gene="ruvA"
                     /locus_tag="VYO97_000519"
     CDS             20935..21528
                     /gene="ruvA"
                     /locus_tag="VYO97_000519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1753378.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21538..22101
                     /locus_tag="VYO97_000520"
     CDS             21538..22101
                     /locus_tag="VYO97_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1753379.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLYDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22098..22775
                     /locus_tag="VYO97_000521"
     CDS             22098..22775
                     /locus_tag="VYO97_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1753380.1"
                     /translation="MMSNKNKGILIFAILYTILFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(22912..23943)
                     /locus_tag="VYO97_000522"
     CDS             complement(22912..23943)
                     /locus_tag="VYO97_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1753381.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDAYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(24198..24881)
                     /locus_tag="VYO97_000523"
     CDS             complement(24198..24881)
                     /locus_tag="VYO97_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1753382.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDIDCSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(24893..25837)
                     /locus_tag="VYO97_000524"
     CDS             complement(24893..25837)
                     /locus_tag="VYO97_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1753383.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            25969..28800
                     /gene="uvrA"
                     /locus_tag="VYO97_000525"
     CDS             25969..28800
                     /gene="uvrA"
                     /locus_tag="VYO97_000525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1753384.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVVEKIQKDGYVRVRVDGEVYDVTEVPDLSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDSSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVEMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVNNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGENGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28793..29854
                     /locus_tag="VYO97_000526"
     CDS             28793..29854
                     /locus_tag="VYO97_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1753385.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            29986..30384
                     /gene="spx"
                     /locus_tag="VYO97_000527"
     CDS             29986..30384
                     /gene="spx"
                     /locus_tag="VYO97_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1753386.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30450..31019
                     /locus_tag="VYO97_000528"
     CDS             30450..31019
                     /locus_tag="VYO97_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1753387.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            31106..31372
                     /locus_tag="VYO97_000529"
     CDS             31106..31372
                     /locus_tag="VYO97_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1753388.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31376..31795
                     /gene="ruvX"
                     /locus_tag="VYO97_000530"
     CDS             31376..31795
                     /gene="ruvX"
                     /locus_tag="VYO97_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1753389.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            31811..32116
                     /locus_tag="VYO97_000531"
     CDS             31811..32116
                     /locus_tag="VYO97_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1753390.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32422..33672
                     /locus_tag="VYO97_000532"
     CDS             32422..33672
                     /locus_tag="VYO97_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1753391.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGDLAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33669..34214
                     /locus_tag="VYO97_000533"
     CDS             33669..34214
                     /locus_tag="VYO97_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1753392.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            34360..35892
                     /gene="cls"
                     /locus_tag="VYO97_000534"
     CDS             34360..35892
                     /gene="cls"
                     /locus_tag="VYO97_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1753393.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            35970..36113
                     /locus_tag="VYO97_000535"
     CDS             35970..36113
                     /locus_tag="VYO97_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753394.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            36150..37688
                     /locus_tag="VYO97_000536"
     CDS             36150..37688
                     /locus_tag="VYO97_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1753395.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
BASE COUNT        10961 a         7167 c         8365 g        11201 t
ORIGIN      
        1 gtgttttgag ctgacttcat cagttctatc tgcaacctca aaacagtgtt ttgagcaacc
       61 tgcggctagt ttcctagttt gctctttgat tttcattgag tattattaag acaatatgag
      121 gagaaagatg aaagaaaaag gattttagga gggggcgcag gcagccatgc caacggctct
      181 tggctatgtc agtatcggcc tggcctgtgg gactatcggt gcgccctatg tgacacctgt
      241 tgagatgggc ttgatgagtc tctttgttta tgctgggagt gcccagtttg ccatgttggc
      301 actgattgtg gttcaagctc ctgtggcagc tattgctatg acggtttttc taatcaactt
      361 gcgtctcttt ttgttgagtt tacacgcatc gacttatttc cgtcatacca gtctttggta
      421 caatatcggt atgtctagta tcttgacaga tgagacctat ggcgttttga tgggtgaatt
      481 ggcccataca gacaaggtaa atcctatgtg gatgcacgga aacaatctta acagctatgt
      541 ggcttggttt gtggggacag tagtcggaac ggctctgggt ggcctgctac caaatccaga
      601 aatctttggc ttggattttg ccctggttgg gatgtttatt ggtatttttg cttcgcaatt
      661 tcagattatg caaagacgga ttcctgtccg caatctgctc attatcctag cagttgttgc
      721 ggtgtccttc tttttgctct tgacagtggt gtctcagtca ctagctgttc tgtttgcgac
      781 gctacttggt tgtagcatgg gggtggtttt agatggtcag taagtatctt ttattagcag
      841 ttattttctc tggcttggtg acttggattc tccgtatgat tcccttcatc ttagtcaagt
      901 ataagggctt gccggcaatc gttgagcgtt ttttgaagtt cttgcccgtt tccattatct
      961 ttgccctgat tctttcaagc gtagtgacag gtaaggttgg cagtcttcct caaatcaaat
     1021 gactggactt cttagccgtc tttccaacag cttgggtagc ctttcgctac cgcaatctag
     1081 tcggaacagt tctctttgga gtggtcttga ttgccatctt gcgtttggtc ttttaaatta
     1141 cccataaaaa aaacttatca cagagataga tatcatataa tggcgtaaat gctctttttc
     1201 tgttaagatt ataaggtatt ctattttgga ggaaatgaca tgaaaaaaat cgttaaatac
     1261 tcatctcttg cagcccttgc tcttgttgct gcaggtgtgc ttgcggcttg ctcaggtggt
     1321 gctaagaaag aaggagaagc agctagcaag aaagaaatca tcgttgcaac caatggatca
     1381 ccaaagccat ttatctatga agaaaatggc gaattgactg gttatgagat tgaagtcgtt
     1441 cgcgctatct ttaaagattc tgacaaatat gatgtcaagt ttgaaaagac agaatggtca
     1501 ggtgtctttg ctggtcttga cgctgatcgt tacaatatgg ctgtcaacaa tcttagctac
     1561 actaaagaac gtgcggagaa atacctctat gccgcaccaa ttgcccaaaa tcctaatgtc
     1621 cttgtcgtga agaaagatga ctctagtatc aagtctctcg atgatatcgg tggaaaatcg
     1681 acggaagtcg ttcaagccac tacatcagct aagcagttag aagcatacaa tgctgaacac
     1741 acggacaacc caactatcct taactatact aaggcagact tccaacaaat catggtacgt
     1801 ttgagcgatg gacaatttga ctataagatt tttgataaaa tcggtgttga aacagtgatc
     1861 aagaaccaag gtttggacaa cttgaaagtt atcgaacttc caagcgacca acaaccgtac
     1921 gtttacccac ttcttgctca gggtcaagat gagttgaaat cgtttgtaga caaacgcatc
     1981 aaagaacttt ataaagatgg aactcttgaa aaattgtcta aacaattctt cggagacact
     2041 tatctaccgg cagaagctga tattaaataa tttcttgaaa tcatccattc tgagtaaggt
     2101 gggtggtttc tcagttttta gggatatagt tttaaactat atatctgact atctaataac
     2161 aatttgtgaa atcaaacaaa ttgtgagata ctagtacggt attattttta aggagaaaga
     2221 atcatgaaaa tcaaaaaatg gcttggtcta gcagcccttg ctacagtcgc aggtttggct
     2281 cttgcagctt gcggaaactc agaaaagaaa gcagacaatg caacaactat caaaatcgca
     2341 actgttaacc gtagcggttc tgaagaaaaa cgttgggaca aaatccaaga attggttaaa
     2401 aaagacggaa ttaccttgga atttacagag ttcacagact actcacaacc aaacaaagca
     2461 actgctgatg gcgaagtaga tttgaacgct ttccaacact ataacttctt gaacaactgg
     2521 aacaaagaaa acggaaaaga ccttgtagcg attgcagata cttacatctc tccaatccgc
     2581 ctttactcag gtttgaatgg aagtgccaac aagtacacta aagtagaaga catcccagca
     2641 aacggagaaa tcgctgtacc gaatgacgct acaaacgaaa gccgtgcgct ttacttgctt
     2701 caatcagctg gcttgattaa attggatgtt tctggaactg ctcttgcaac agttgccaac
     2761 atcaaagaaa atccaaagaa cttgaaaatc actgaattgg acgctagcca aacagctcgt
     2821 tcattgtcat cagttgacgc tgccgttgta aacaatacct tcgttacaga agcaaaattg
     2881 gactacaaga aagcactttt caaagaacaa gctgatgaaa actcaaaaca atggtacaac
     2941 atcattgttg cgaaaaaaga ttgggaaaca tcacctaagg ctgatgctat caagaaagta
     3001 atcgcagctt accacacaga tgacgtgaaa aaagttatcg aagaatcatc agacggtttg
     3061 gatcaaccag tttggtaata agaaacaggg aggtgggaga gagaattcca cctcttgctt
     3121 ttgtatagag cacggagtgt aaagaagact attcgttcat agaaaggtag agagaatatg
     3181 gtttttccta gcgaacaaga acagattgaa aaatttgaaa aggatcatgt agcccagcat
     3241 tattttgagg ttttgcgtac cttgatttct aagaaatcag tctttgccca gcaggttgga
     3301 ctcaaggaag tcgcaaatta tctgggtgag attttcaagc gtgttggagc tgaagtggag
     3361 attgatgaga gctatacagc gccctttgta atggcacatt tcaagagttc gcgtccagat
     3421 gccaagacct tgattttcta taaccactat gacactgtgc cagcggatgg ggatcaggtc
     3481 tggacagagg atccttttac gctttcggtc cgcaatggct tcatgtatgg gcgtggagtt
     3541 gatgacgaca agggtcatat cacagctcgc ttgagtgctt tgagaaaata tatgcagcac
     3601 catgatgatt tacctgtcaa tatcagcttt atcatggagg gagcggagga atcggcttca
     3661 acagacctag ataagtattt ggaaaagcat gcagacaaac tccgtggggc ggatttgttg
     3721 gtctgggaac aagggaccaa aaatgccttg gaacagctgg aaatttctgg tggcaataag
     3781 gggattgtga cctttgatgc caaggtaaaa agcgctgatg tggatatcca ctcgagttat
     3841 ggtggtgttg tggaatcagc tccttggtat ctcctccaag ccttacagtc tcttcgtgct
     3901 gcggatggcc gtatcttggt tgaaggcttg tacgaagaag tacaagagcc caatgaacga
     3961 gaaatggcct tgctagaaac ttatggtcaa cgaaacccag aggaagttag tcggatttat
     4021 ggattggagt tgcctctctt acaggaggag cggatggcct ttctaaaacg tttctttttc
     4081 gagccagcgc ttaatatcga aggaatccag tctggttatc aaggtcaggg tgttaagact
     4141 attttacctg cagaagccag tgccaagcta gaggttcgtc tggttccggg cctagaaccg
     4201 catgatgttc tggaaaaaat tcggaaacag ctagacaaaa atggctttga taaggtagaa
     4261 ttatactata ccttgggaga gatgagctat cgaagcgata tgagcgcacc agccattctc
     4321 aatgtgatcg agttggccaa gaaattctat ccacagggcg tttcagtctt gccgacgaca
     4381 gcggggacag gacctatgca tacggtcttt gatgccctag aggtaccaat ggttgcattc
     4441 ggtctaggaa atgccaatag ccgagaccac ggtggagatg aaaatgtgcg aatcgctgat
     4501 tattacaccc atatcgaatt agtagaggag ctgattagaa gctatgagta gagatattat
     4561 caagttagat cagatcgatg tgacttttca ccaaaagaag agaaccatca cagcggttaa
     4621 ggatgtgacc attcacatcc aagaagggga tatctacgga atcgttggat attctggagc
     4681 agggaaatca acccttgtac gggtgattaa tctcttgcaa aaaccatctg cagggaaaat
     4741 taccattgac gacgatgtga tttttgatgg taaggtcacc ttgacggcag agcagttgcg
     4801 tcgtaaacgt caagatatcg ggatgatttt ccagcatttt aacctgatga gtcaaaagac
     4861 agcagaggag aatgtagcct ttgcccttaa acactctgga ctcagcaagg aagaaaagaa
     4921 ggctaaagta gctaagttgt tggacttggt tggtttggca gatcgtgctg aaaactaccc
     4981 ttcacaacta tctggagggc aaaaacagcg tgtggcaatt gcgcgtgcct tggccaatga
     5041 tccaaaaatc ttgatttcag acgagtcaac ttctgccctt gaccctaaga caaccaagca
     5101 gattttggcc ttgttgcaag atttgaacca aaaattagga ttgacagttg tcttgattac
     5161 gcatgaaatg cagattgtca aagacattgc caaccgtgtg gcggttatgc aggatgggca
     5221 tttgattgaa gagggaagtg tccttgaaat cttctcaaac cctaaacaac ctttgactca
     5281 agactttatc tcaacagcca caggtattga cgaagccatg gtcaaaatcg agaagcaaga
     5341 aatcgtggaa cacttgtctg aaaacagtct cttggtgcaa ctcaagtacg ctggagcttc
     5401 aacagacgag ccacttttga atgaattgta caagcattac caagtaatgg ctaatattct
     5461 ctatgggaat atcgaaattc tcgatggtac tcctgttgga gaattggtgg tggttttgtc
     5521 aggtgaaaaa gcagcgttgg caggtgccca agaagccatt cgtcaagcag gtgtacaact
     5581 aaaagtattg aagggagtac agtaagatgg aatcattgat tcaaacctat ttaccaaatg
     5641 tctataaaat gggttgggct ggtcaggcag gctggggaac ggctatctac ttaactcttt
     5701 atatgacagt tctttccttc attatcggag gcttcttggg gctagtggca ggtctctttc
     5761 tcgtcttgac agcgccaggt ggtgtcttgg agaataaagt cgtattctgg attttagaca
     5821 aaattacctc aatttttcgt gcggttccct ttatcatcct cttggcaatc ttgtcaccac
     5881 tttctcactt gattgttaaa acaagtatcg ggccaaatgc agcccttgtc ccactttctt
     5941 ttgcagtctt tgccttcttt gcccgtcagg tgcaggttgt cttggctgaa ttggatggcg
     6001 gtgtcattga ggcggctcaa gcgagcggag cgactttctg ggacatcgtg ggtgtttacc
     6061 tatcagaagg tcttccagat ttgatccgtg tgacgactgt gaccttgatt tcccttgttg
     6121 gggaaacagc tatggccggt gcggttggag ctggtggtat cggtaacgta gccatcgctt
     6181 atggatttaa ccgctacaat cacgatgtga ccatcttggc aaccatcatt atcattttga
     6241 ttatctttgc aatccaattc ttgggagatt tcttgactaa gaaattgagc cataaataaa
     6301 aaagagccgt gtggctcttt ttaactgatc agattttctg ggcaaatttt ttactcaagg
     6361 cttgtccaat caaggcaccc actagggctc cgatgacaat acttgcgata aatagaagga
     6421 cagttccagg gtttggagtg accatgatac ggtcgatata ttcttgggat tttcctcttg
     6481 ccagaagagt agccatatag gctttgggcg caatccacat aagcaagatt ggtcctgttg
     6541 tactaaaggc gaaaataatg aaagaaagga agttctttgt tttgtccttg tattttccta
     6601 aatgagctac ttcatctgct aggaggccac agataattcc aggaaggaag gaaccagcac
     6661 cgtgtttagt tcccaagaaa aagaaggcaa tgacaaggcc gatagtggta atggctccaa
     6721 agcgcggaac ttttgcgact aggatcatat agacgctacc gccgacaagg gcagtaaagg
     6781 caggcgcata aaacatgttt ccagtttggt caaagagatt gcccaaaagg acaccaatcc
     6841 ccatgcagag gaagtaaaga actgcaaaaa gcagtgtagt taagatagat tttttcatga
     6901 attgtctcct gataattttt tcacaattct cattgtacca cggtttggtg ggattgtaaa
     6961 tggatactag aagtttttga aaacgcttga aatatgatat aatggtttca tagttatttt
     7021 taggggaata tcatgggtag atttttagac tttgtcttta atcgtttctt tttagggatg
     7081 attgcgacag ccttcttttg gctattaact ttagcaggag ggattatcct tggtctagcg
     7141 ccggctagtg ccaccttgat gagcttatat gcagaacatg gttatagctt tcgggaatac
     7201 agtttgaagg aggcttggtc tctttacaag caaaattttg tctcaagcaa cctgattttc
     7261 tatagctttt taggtgtggg tctagttttg acctatggtt tgtatctctt ggtgcaattg
     7321 cctcatcaga ccattgttca tttgattgcg acccttttga atgtcctagt agttgccctg
     7381 atctttttgg cttatacagt atctttaaaa ttacaagttt attttgcctt gtcctatcga
     7441 aatagtctca aattatcctt gattggcatc tttatgagtc tagcagctgt ggccaaggtt
     7501 ctccttggga ctgtgctact tgtagcaatt ggttactata tgcctgccct gctatttttt
     7561 gtaggaattg ggatgtggca tttctttatc agtgatatgt tggaacctgt ctatgaaatc
     7621 atccatgaaa aattggcgac aaaatagaat gaagcagttt tggctacata cgcttctaag
     7681 aacctatagt tcagtgatga tcattatcat tgcgagtttt gcaatcttac tctcttacgc
     7741 tgactgggat tcacgtgaaa aggaagccca gagagtagcc cagcgtgtaa ctgctagaac
     7801 agtgagtgaa attgaatatt accatagaga gtcaacccag atagctcagg ctttagttga
     7861 aaatcaagct cgtatcgagg gaatctataa atactttagc cttagcatgc cagactattt
     7921 ttactggcaa ttagagcgga aagcttcgcc ttatatatca gtctctctgt atgaaaatgt
     7981 tgatgacctc tatgttcgaa atgattttgt aactggggtg gccattgctt ttcaagatta
     8041 caaggaagtc tatgtttcta ctaaagacaa acgtagtgga gaaaaaatca gggctgagga
     8101 tttcaaacca gcaggaaata gttttgccat tccagtgtca gatccagtgt cagatcaaga
     8161 cttaggagtg atttacatct ccttggatcc tgctgtttta taccatgcca ttgataatac
     8221 tagaggtcat actccgatgg cagtaacagt gacctcacct tttgatacgg agatttttca
     8281 tatgggtgag acagttgata aggagagtga aaattggcta gttggcttaa cttctcatgg
     8341 ttatcaggtt caggtggcag ttcccaaaaa ctttgtttta caaggaacgg tgaccagctc
     8401 tgctttgatt gtgggcttga gccttctctt tattgtcatt ctttatctga ctttgaggca
     8461 gacctttgct aattatcaaa agcaggtagt ggatttggtg gattccatcc aagctattgc
     8521 ccaaggacaa gaaggtcttc gcattgatac gcttgaaaag gatcaggaat tgctcctaat
     8581 cgcggagacg accaatgata tgttggatcg attggaaaag aatatccatg atatttacca
     8641 gttagaactc agtcaaaaag atgccaatat gcgggccttg caggcgcaaa tcaaccctca
     8701 cttcatgtat aatacgctgg agttcttgcg tatgtacgca gtcatgcaga gtcaagatga
     8761 gttggcagat atcatttatg aattcagtag tctcttgcgt aacaatattt ccgacgaaag
     8821 agagaccctc ctcaaacagg aattagaatt ttgccgtaaa tacagctatc tctgcatggt
     8881 tcgctatccc aagtccattg cctatggttt caagatagat ccagagttag agaatatgaa
     8941 gattcccaag tttaccttgc aaccgctggt agaaaactat ttcgcgcatg gtgttgacca
     9001 caggcggaca gataatgtga ttagcatcaa ggctcttaaa caggatgggt ttgtggaaat
     9061 tttggtggtc gataatggta gaggaatgtc ggctgaaaag ttggcaaata tccgagaaaa
     9121 attaagtcag aaatattttg aacaccaagc cagctacagt gatcaaaggc agtctatcgg
     9181 gattgtcaat gtacacgagc gttttgtgct ctattttgga gaccgctatg ccattactat
     9241 agagtctgca gagcaagccg gtgttcagta tcgtattaca attcaagatg agtagaaagg
     9301 gagaaaatgt ataaagtatt attagtagat gatgagtaca tggtgacaga aggtctgaag
     9361 cgtttgattc cctttgataa gtgggatatg gaggtcgtcg caacagccag tcatgccgat
     9421 gaagctctag aatatgttca ggaaaatcct gtcgatgtca tcatttccga tgtcaatatg
     9481 ccagacaaaa cagggcttga tatgattcgg gagatgaaag agatcttacc agatgctgcc
     9541 tatatcctgc tctcaggtta tcaggagttt gattatgtaa aaagagcaat gaaccttagt
     9601 gtggtggact atttggtcaa gcctgttgat aaggtagagc tgggaaatct gctggagaag
     9661 attgcaggtc agctcggcga gagagggaag aaaagtcaga ctcttagtca agaattagac
     9721 gaggctggat ttgttagtta tttaggggat aaggagaatt ggtggatagg tctatccaag
     9781 gaaaaacaag gttccttcac cattccctac tatgtcttgg gccaagactg gcagattttc
     9841 atttctgacc aacccctaga tggtttagtc gttacacctt ttgaagctcc ttatcaagaa
     9901 cactttgaac gctggaagct gaatgctgag aaaaccctct tttacggttc tgtaaatttg
     9961 cagcagtctg agagtctctt tgcctattac gaaccgattt atagggttat tattcaggga
    10021 aatctcaatc aaatcgtaga agagttaaat ctcttggaga aggtagttct tgaaaatacg
    10081 ccgcgtgttc cgattactaa acagcttttt attcagtttg tcatggatgt tttccattta
    10141 tttgaacatc tcaaagctga tgacctgaca gacatcgtca aaaccattca tgctattcaa
    10201 tccttcgatg aattggttcc ttatatcaag gaaactctga gcagcttttt cggtcaatac
    10261 cgtatgaatg aaaatgtggt cagtgtgctg gaagtcattg gtcgtgatta ccaaaaagag
    10321 ctttccctca aggatatcag taaggctctc tttatcaatc ctgtctatct agggcagttg
    10381 attaagcgtg aaaccgattc gacttttgca gagttactaa acaaacaacg tattaaggct
    10441 gcccaacaac ttttgctttc aactagtgac agcatcgaag atatttgtta tgctgttggt
    10501 tacagtaacc ttggatattt ctataaagtt ttccgaaaat tgtgcggaaa atcgccaaaa
    10561 gcctaccgaa aacaggtaga aactatacta taagatttgt attcctttac aaaaggtgct
    10621 ataatattat caggaggtcc tatcatgaaa aagaaaccaa tttatctatg ggttttgcta
    10681 atcttgtctg cccttatttc agttccgtct ctgtttggaa ttgtgagtcc cttgcctagt
    10741 aaagaagccc ttcgtgctgc tcagaaacaa gttgcagggg tcaatgctca gcaattagaa
    10801 gaccagctta attataccta tagagtagca gaagcatctc attctatttt taatgttgct
    10861 ttgattgtgc tatctactat tttagtggtc gtagcgattg tcttccttgt tcgtaaaaat
    10921 ttgcaatatg caaactatac ttacgttggc tatgttttgt tagctattat tggttcgatt
    10981 tatggctatg ttggtttaca agacgctgtg cagttggttc aagatgagag catgcgtttg
    11041 acagtgagta ttggttcaaa agctgttagc attttctata tcgtcatcaa tgttctcttc
    11101 ctagcccttg tcttctataa gatgtggcga caacagaaag ccttggcaga agaagaggaa
    11161 acagaagagc ttacctaagt attgacaaaa aagaactatc aagatacaat cttggtagtt
    11221 gttttttgat tatagataaa atcatggagg tacatatgaa gacaatttct ttagtttata
    11281 ttagtttgag tggcaacact gaaagttttg tgacgcgctt gaaagactat ctcttgtccc
    11341 agtacaaagg gattgaggtt caaaagattc atatcaagga tttggtcaag gaaggcaaaa
    11401 atttctatga aatggaccat ccctatgtcg cttttttgcc gacctatctc gaaggtggga
    11461 atggcgtgga taacggagat gttgagattt tgacgacacc ggtgggagat tttattgcct
    11521 atggtaacaa tgctagtaag tgttttggtg tggttgggtc aggaaatcgt aactttaata
    11581 accaatactg cctaacagcc aagcaataca gtcaacgctt tggcttccct gtattggctg
    11641 actttgaaat gcgaggcatg ctggaagata tcaaacatgt cgcagctatt atcgcagatt
    11701 tgtatgagtt ggaaaaggag aattaatact caatgaaaat caaagagcaa actaggaagc
    11761 tagtcgcagg ttgctcaaag cacagctttg aggttgcaga tagagctgac gtggtttgaa
    11821 gagattctcg aatagtataa agttcttata agaggtggtt gaagttgttc aactgttttt
    11881 tgagtataaa cagtctttaa gaacgtaaaa aagcataagt tcaggtcttc aaaaccttgt
    11941 tcttatgctt tttatcatag aaatatttgc tgaattttct cttgaaatga aaccttatct
    12001 tcatctcgcg cttgtccctt accctgcttt tccgattgcg agtgcgtaga taaagaagat
    12061 ggcgaagcca aagaggaaaa tcaatcccgc aatggcaagg tgtttgagcc aagaaggaag
    12121 atttttgttt tcacgcgtta ccaaggcgta attcaaaaga gcaaagaaag gtgttgtcag
    12181 gaaagagcca atcatggcaa agcggagcat ggttgaaacc tgaccagcga agaacttgat
    12241 aatgacgata ccgatgatag cagtgatggt catccagatg ttcaaagatt tacgattgtc
    12301 ctctttttga ctgattagca gtcggagaga ttcctgatta acgcgagaat agccatcgat
    12361 aacagttata actgttccaa agatacagag gaaggcaata aaggtaatca agtaacggga
    12421 ccattcgcca agaacagagg catacatgcc cacgaattga gagatgtatt tggctgaagc
    12481 agcttcaacc gcctgccctg taggatactg aatcagtgct cccagtgcca caaagaagac
    12541 ggctaggata gctgttccaa tataaccagt gttaaagtca aacagagcgt cttctgtgtt
    12601 aaagttgacg gtctttctct tttcagctga ccaaagtgaa ttgatggctg aaatttcaat
    12661 aggagccggc atccatccta ggagggagac gatgaagggc agagctgcca tttgccaagg
    12721 tgtcttctcg acaaaatcag aactgtattc tggatgcttg accgccgcaa tgataactgc
    12781 aagaacagtc gcaatggtta aggcagacat aatccatttg accatgccgt ctaaaagttt
    12841 gtagcctcca aagagtagca tagcccaaat gattgcaacg agaatgaggg accactgagt
    12901 aatgctaagt ccaatcattg ggaaggcact ggcgatgata gctgagcaca gaatggcaac
    12961 accagccgtg ttgaccatag ccgaaaagac attgaggata aagaaaatcc agagatagag
    13021 ttttcctttt tcggcataac cttcaaccaa agtctttcca gtatcagctg tgtattcagc
    13081 accaaaacgg aaaaatggat atttaaagac attggctaag atgaccaaga gaagtagaga
    13141 ccaaccgtaa gaaccgccag cttgagttga ggatacaatg tgggaacctc caacagcggc
    13201 agttgccatt aggattccag gtcccattgc ttttagcttg cttgcccagg ttgattgatt
    13261 taaggaaata gcttgcgaca tgataaatac tcctttttga attttcagaa taatctgtat
    13321 atgtaatcta tattctacaa aaaattttgg aaaaatgcaa gagtattttg aaaaaagata
    13381 gaaaatttac aaagaaaatc tgaaataact gttatgatat agaggattga aactagaata
    13441 gtacacctct gcttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttatc
    13501 ctgttcttat ttcatttcac tatagtaagg tgcagcagga taaagaaaaa ccgagaagtt
    13561 tctttctcgg atttgcttat tatgaggttc ctattttcta tgctccaaga gttgattgat
    13621 gtgatctact tttttagcct ctcttttata gagaatagcc cagatgatga gatagacaat
    13681 cgcaaactcg ataatgagct ggaggtagaa aatccagtgg aaagggaacc aaccagcaag
    13741 agttgctagt gggacaaagc cagccagcat aaggaagaaa tgagtcagag tggcacgaag
    13801 catgctccag tcacggctaa ataagcggtt gccaaagttg aagagcatac cgatggttgc
    13861 ccagataagt gtgcagtaga gcaagaccag ggcaccgtga acctgatgct gatccatcac
    13921 ttggccgatg agagagtagg gatttagtgg tgcgtaggta tttggtgcat aaatgagtga
    13981 aaagaggata gagaggatga ggccgataag aacaccggta gctgcatcgt gaaatacttg
    14041 ttttttcata gttctaattt ctccttgatg gtttttagat aacggcgtga agagtaggtg
    14101 aagctttcgt ttttcaagaa aatttctacc agaccgtttg gcgtgagctt gaggtgagag
    14161 atggaatcga tattgatgat ttctgattgg gaaatttgga taaaattggt tggcaagagt
    14221 ttaagaacct gatagagtcg caaatcaatg ctgtaggtct gactcgcggt ttctgctaga
    14281 accttccgat tctcgatata gaagcgctga atcttgccaa tctcaactag atagacctga
    14341 tcatcgattt ttcctttgat tttttctctt tggtccagat tttctgcgaa ctcgatgact
    14401 ttctggactt tttcggtttc ttgaggtgct tggacaatca gcttttcctc ctcgtaagtc
    14461 tcactaatct gtagttctac tttcataaat ttctctcctt gtttttcata caaaattaat
    14521 ctcacttctt gtacaccttt attaatactc ttcaaaaatc tcttcaaatc acgtcagcgt
    14581 cgtcttgccg taggatagtt gttgactttg tcagttttat ctacaacctc aaaacagtgt
    14641 tttgaactgt ctgcgcctag ctttctagtt tgctctttga tttttattga gtataccact
    14701 attttactcc ctctggcaag ggactttgtc tatgtggagg gattgggctc ctatgtggtg
    14761 gagcttttct gttctttctg aaatatggta taatagcact aattaatttc taggaaaata
    14821 gatacagaaa ggggctgaaa gatgtctcat attattgaat tgccagagat gctggcaaac
    14881 caaatcgcag ctggagaggt cattgaacgt cctgccagtg tggtcaaaga gttggtagaa
    14941 aatgccattg acgcgggctc tagtcagatt attattgaga ttgaggaagc tggtctcaag
    15001 aaggttcaaa tcacggataa cggtcatgga attgcccacg atgaggtgga gttggccctg
    15061 cgtcgccatg cgaccagtaa gataaaaaat caagcagatc tctttcggat tcggacgctt
    15121 ggttttcgtg gtgaagcctt gccttctatt gcgtctgtta gtgtcttgac tctgttaacg
    15181 gcggtggatg gtgctagtca tggaaccaag ttagtcgcgc gtgggggtga agttgaggaa
    15241 gtcatcccag cgactagtcc tgtgggaacc aaggtttgtg tggaggatct ctttttcaac
    15301 acgcctgccc gtctcaagta tatgaagagc cagcaagcgg agttgtctca tatcattgat
    15361 attgtcaacc gtctgggctt ggcccatcct gagatttctt ttagcttgat tagtgatggc
    15421 aaggaaatga cgcggacagc agggactggt caactgcgcc aagcaatcgc agggatttac
    15481 ggtttggtca gtgccaagaa gatgattgaa attgagaact ctgacctaga tttcgaaatt
    15541 tcaggttttg tgtccttgcc tgagttgact cgggctaacc gcaattatat cagtctcttc
    15601 atcaatggcc gttatatcaa gaacttcctg ctcaatcgtg ctattttgga tggttttgga
    15661 agcaagctta tggttggacg ttttccactg gctgtcattc acatccatat cgacccttat
    15721 ctagcggatg tcaatgtaca tccaaccaag caagaggtgc ggatttccaa ggaaaaagaa
    15781 ctgatggcgc tggtctcaga agctattgca aatagtctca aggaacaaac cttgattcca
    15841 gatgccttgg aaaatcttgc caaatcgacc gtgcgcaatc gtgagaaggt ggagcaaact
    15901 attctcccac tcaaagaaaa tacgctctac tatgagaaaa ctgagccgtc aagacctagt
    15961 caaactgaag tagctgatta tcaggtagaa ttgactgatg aagggcagga tttgaccctg
    16021 tttgccaagg aaaccttgga ccgattgacc aagccagcaa aactgcattt tgcagagaga
    16081 aagcctgcta actacgacca gctagaccat ccagagttag atcttgctag catcgataag
    16141 gcttatgaca aactggagcg agaagaagca tccagcttcc cagagttgga gtttttcgga
    16201 caaatgcacg ggacttatct ctttgcccaa gggcgagatg gactttacat catagatcag
    16261 cacgctgctc aggaacgggt caagtacgag gagtaccgtg aaagcattgg caatgttgac
    16321 cagagccagc agcaactcct agtgccctat atctttgaat ttcctgcgga tgatgccttg
    16381 cgtctcaagg aaagaatgcc tctcttagag gaagtgggcg tctttctagc agagtacgga
    16441 gaaaatcaat ttattctacg tgaacatcct atttggatgg cagaagaaga gattgaatca
    16501 ggcatctatg agatgtgcga catgctcctt ttgaccaagg aagtttctat caagaaatac
    16561 cgagcagagc tggctatcat gatgtcttgc aagcgatcta tcaaggccaa tcatcgtatt
    16621 gatgatcatt cagctagaca actcctctat cagctttctc aatgtgacaa tccctataac
    16681 tgtcctcacg gacgtcctgt tttggtgcat tttaccaagt cggatatgga aaagatgttc
    16741 cgacgtattc aggaaaatca caccagtctc cgtgagttgg ggaaatatta aaagtataaa
    16801 aaagtctggg aaaaattttc aaaatcagaa aaacgcataa aatcaggtgt tcaaaaacct
    16861 tgatttttat gcgttttatc atggaaatag ttacttcatt ttttcctaat tcttttcgaa
    16921 actcttttta aacgacgtca gttttatcag taatctcaaa acagtgtttt gagctaattt
    16981 tgccagtttt gtctgtaaca tcgaagttgt gttttaccac tctgcgactg gtttcctagt
    17041 ttgctctatg attttcacag agtattaaat tgcgattttg ccaagtttct ttattcgtct
    17101 aaaagtagag tctgttctat gcgtctaatg tacgaattag gttgaccatt tcaatagctc
    17161 cttgtgcaca ctcagaaccc ttatttcctg ctttggtacc agctcgttct atggcttgtt
    17221 caattgtatc tgtcgttagc acaccaaaca taacaggaat ttcgctattt aaactgattt
    17281 gggcgattcc cttagatacc tcgctacata cataatcata atgacttgta ttccctctaa
    17341 tgacagctcc caagcagata attgcatcat attttttact ttttgccatt tttgatgcaa
    17401 tcagtggtat ttcaaaagct cctggaaccc aggctacctc gatatctttc tcgtttacat
    17461 tctctctttt gagattatct agtgctccag ataataattt tgaagttata aattcattaa
    17521 atctcgctac aacaatacct attttaatat tgtttgctac taaattacct tcataagtgt
    17581 tcatttattt ttcctccata tttaaaatgt gacccattcg atttttcttt gtttctaaat
    17641 aaaaactatt gtaaggattg gcttctattt cgattgatat tctactggaa atggtaattc
    17701 catatttttc taactgttca accttgtcag gattatttgt cagtaaatga agtgactgaa
    17761 gtcccagatc tttaagcatt tgtgctccaa tatgatattc tcttaaatca ccttcaaagc
    17821 ctaatgcaag attagcatca agcgtatcca tgccttgatc ttgtaaatga taggctttta
    17881 atttattgat aagtccaatt cctcgtccct cctgtcgcaa gtaaagtaag acacccgaac
    17941 cattctcaac aatcattttc atagctttat cgaattgctg tccacaatcg caacgtaaag
    18001 agcctaaaat atcccctgtt aaacattcgg agtggacccg acataataca ttggcttcat
    18061 cctctatgtt tcccataata agagcaagat gatgttcccc atttagttta tctatatagc
    18121 taattgcttt gaaattaccg tatctagtag gcatattgac agttgaaact cgttctacca
    18181 gctgatcata tacttttcta tattcttgta attctttgat ggtaattagt ggaatgttgt
    18241 gtttcttcga gaactgaatt aaatcatctg ttctcatcat tttgccatca tgattcatta
    18301 tttcacaaca taggccacac tcttttagtc cagctaattt taataaatca acagttgctt
    18361 ctgtgtgtcc atttctttct aggacaccac ctttttttgc aattaaagga aacatgtgtc
    18421 ctggcctgcg aaaatcagag ggtgttatat cttcagctac acacatacgt gcggtcagtc
    18481 ctctttcctc ggcagaaata cctgtggtcg tttctttata atcaattgaa actgtaaaag
    18541 cagtcttatg attatctgta ttgttttcaa ccataggtga aagcattaat tgattagcta
    18601 aactttcgct cataggcata caaattaatc ctttggcata agtagccata aaattaacat
    18661 tttctgttgt agctgcttgt gcagaacaaa ttaagtctcc ttcattttct ctatccttgt
    18721 cgtctataac aagaacaagt cgtcccttct gcaatgcttc taatgcttct tgtatttttc
    18781 gatattccat tgactaatta tcctttctgc taaaatccat tttgatataa tagttcctta
    18841 gatatttctg attttggaga gttatccatc agtttttgca catatttacc taagatatca
    18901 ttttcaagat ttactgtact cccgacttgt ttactcttaa gaatggtttg ttccaaggta
    18961 tgagggataa cagatactga aaagtttact ttggagactt tagcgacagt cagactaatg
    19021 ccgtcaattg taatagatcc tttttcaact attaaatcta aaatttcttt ttgtgtgttg
    19081 atttgatacc atacagcatt atcatctttt tttattgacg agatttttcc tgtaccatca
    19141 atgtgtcctg taacgacgtg acccccaagt cgaccgttga cagataaggc tctttctaga
    19201 ttcacctcac ttccatgttt taatagagta agagctgttc gactccatgt ttcattcatt
    19261 acatcaactg taaaggattg atgattgaaa tgagtaactg taagacagat accatttact
    19321 gctatactat cgcctaaatg gatatccgtt aatatttttg aggctttaat tgatagttta
    19381 caattacgag agtctttctg tattctttca acttttccga tttcttcaat tattcctgtg
    19441 aacatggata aatcacttca ctttctatga gatagtcatt tcctatttga gaaaaagtat
    19501 aaggtttcaa tctaatagcg tcatttggca aagaaatgcc ttcacctccg acaggaaact
    19561 tggcactgcc tccaaaaatt tttggtgcaa tatatatttt cagctcatca acaatttgtt
    19621 gttccaaagc actccaattc attagactgc ccccttctag aactaggcta tcaatctgca
    19681 tgtttcctag atgttgcatt aaactcgata agtctatatg attgcctttt ttctttatgg
    19741 aaagtatttc acagccatga ttttgatata gcttcatttt atttttgtct tcagaggaag
    19801 tggcaatgta agttttaata tcatttgctg tttttacgat tttagaggta agaggagttc
    19861 gtaaatgtgt atcgcatatg atacggatag gatttttccc ttcctccaat ctacatgtca
    19921 gcaaaggatc gtcttgaata acagtattga ctcccaccat aattgcacta acatggtgtc
    19981 gtaactgatg cacgtgcttt cttgcttctt cttcagtaat ccatttggat tgatttgttt
    20041 tagtggctat ttttccatcc attgacattg catatttcat aaaaacatag ggtacatgct
    20101 gggtaatata ctttctaaaa ctttttatta agttaagaca ctcattttct aaaattccaa
    20161 cagtaacttg aagattattt tcctcaagta tctttactcc ttttccagat acaataggat
    20221 tacagtctag gcttccaatg actactcttg taataccact atcgattata gcatctatac
    20281 agggaggtgt tttcccgaag tgacaacagg gttcaagtgt tacataaagc gtcgctccga
    20341 caggggattt tctacagttt ttaagagcat ttctctcagc atgtgggcca ccaaaaaact
    20401 catgataacc ttgtccgata atgtgattat cttttacaat aattgcgccg accataggat
    20461 tggggttgac gtaaccagcc cctttttgtg ccaattttat tgctaatttc atatattttg
    20521 aatcgctcat ctcgctacct ccaaaaaaat ataccttgaa taggggacta ctcaaggcat
    20581 acaaaagaaa acttatgcga ttaacaaaaa tgctctgaaa tgacaagtaa tcatttcaga
    20641 gcacgcaaaa agcacaaata tacttttatc ttctttcatc cagactatac tgtcggcttt
    20701 ggaatttcac caaatcatgc ctttcggctc gtgggctata ccaccggtag ggaattgcac
    20761 cctgccctga agatagttat tcaattacag atgattatag tacttaattt tgaatatgtc
    20821 aacagataaa taccgattgt ttttgatata ctgtatttgt gataatcgat tctcgctcct
    20881 cggataaaga aaatatgata tactagataa acgaaataag agagaaggaa cactatgtac
    20941 gcatatttaa aaggaatcat taccaaaatt actgccaaat acattgttct tgaaaccaat
    21001 ggtattggtt atatcctgca tgtggccaat ccttatgcct attcaggtca ggttaatcag
    21061 gaggctcaga tttatgtgca tcaggttgtg cgtgaggacg cccatttgct ttatggattt
    21121 cgctcagagg atgagaaaaa gctctttctt agtctgattt cggtctctgg gattggtcct
    21181 gtatcagctc ttgctattat cgctgctgat gacaatgctg gcttggttca agccattgaa
    21241 accaagaaca tcacctactt gaccaagttc cctaaaattg gcaagaaaac agcccagcag
    21301 atggtgctgg acttggaagg caaggtagta gttgcaggag atgaccttcc tgccaaggtc
    21361 gcagtgcaag caagtgctga aaaccaagaa ttggaagaag ctatggaagc catgttggct
    21421 ctgggctaca aggcaacaga gctcaagaaa atcaagaaat tctttgaagg aacgacagat
    21481 acagctgaga actatatcaa gtcggccctt aaaatgttgg tcaaatagga gcagagaatg
    21541 acaaaacgtt gttcgtgggt caagatgacc aacccgctct acatcgccta tcatgatgag
    21601 gagtggggcc agcccctcta tgatgaccaa gcattgtttg agttgttgtg tatggaaacc
    21661 tatcaggcag gcctgtcttg ggaaacggta ctcaacaaac gccaagcttt ccgagaagcc
    21721 tttcatagct atcaaattca ctcagtcgca gagatgactg acactgaatt ggaagccatg
    21781 ctggagaatc cagctatcat tcgaaataga gccaagattt ttgctacacg cgctaacgcc
    21841 caagcttttc tacagttaca ggcagagtac ggctcttttg atgcctatct ttggtctttt
    21901 gttgagggga aaactgtcgt taacgatgtt cctgattatc gccaagcccc agctaaaaca
    21961 cccttatctg agaaattagc caaagatctc aaaaaacgag acttcaagtt cacaggccca
    22021 gtcgccgtat tgtcttttct acaggctgca gggctagttg atgaccacga gaatgattgt
    22081 gagtggaaag gtcttaaatg atgtctaaca aaaataaggg aattctgatt tttgcgattc
    22141 tctatacaat cctctttgtg tttgatggcg ttaaattgct ggcttcttta atgccatctg
    22201 ccattgcaaa ttatcttgtt tatgtagttt tagctttata tggctccttc ttgttcaagg
    22261 atagattgat ccaacaatgg aaggagatta gaaagactaa aagaaaattc ttctttggcg
    22321 tcttaacagg atggctcttt ctcattctga tgactgttgt ctttgaattt gtatcagaga
    22381 tgttgaagca gtttgtggga ctagatggac aaggtctaaa tcagtctaat attcaaagta
    22441 cctttcaaga acaaccacta ctgatagctg tttttgcttg tgtcattgga cctctggtag
    22501 aagaattatt tttccgtcag gtcttattgc attacttgca ggaacggttg ccaggtttac
    22561 taagcattat tctggtagga cttgtttttg ctctgactca tatgcacagt ttggctctat
    22621 cagagtggat tggtgcagtt ggttacttag gtggaggcct tgccttttct attatttatg
    22681 tgaaagaaaa agagaatata tactatcccc tacttgttca catgttaagc aacagcctct
    22741 ccttaatcat tttagctatc agtatagtaa aatgaaatga gaacaggaca aatcgatttc
    22801 taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatatattgt aatatgtgat
    22861 gaaaatgcca gtaatgatac cgagaaaaaa gctgagaaac ttttcccagc tttatttgtt
    22921 atagtcaaag agaatgactt gttcctgtgc atctacatga gcatggaccc caaagggtac
    22981 aattgctctt ggagttgcgt ggccgacatt cagattatag acaatcggga tattgctgtc
    23041 aatgatatcc aatagtgcct ctttatagtc gtcatggaaa gtttcatcca taggttttcc
    23101 gaccaagagt ccattgatga ccgcgaatat gccagtgtcc tttaaagtta gcaacatctt
    23161 tttgaagtct tctggcttag gcttttcttc gcttgtttcg agcaagagga ttttcccttc
    23221 ccagtctgac aagtcaggga aaagtttgta tttttggcag agttccgtgc tatctgcgta
    23281 tcgagagttg tcaaagatat cgtagaggga ttcgaggcaa ccaccgagga ttttcccctc
    23341 gaactgggca cttccttgca acaagtcaaa acctgtattt gtatgactga cacgaggtgt
    23401 tcccagggcc gtgggactaa aatcagttcg ttcctcatac caaacgtcac tagggcggat
    23461 ttctgaaatt cttcccgtct caatcaattc tttaaagtag tgaaggctat aggctagcat
    23521 ttctttgtct aattcacaaa tgtctgctaa aaaggattga ccataaaaag tcttgattcc
    23581 taatttatgc aacatgaggt ggttcatggt tgtatccgag aagccaagaa aaattttttg
    23641 tttgataacc ttttggagtt ggtcattttc aaaaagataa ggtagcaagc gataggcatc
    23701 gtctccaccg atggcacata ggatcatgtc gatgctatca tcagaaaagg catgaatcaa
    23761 atcctctgca cgagcttcag gatggtcctt gataaagtct aatcctttta acgaatgggg
    23821 caaaaagatg ggattgagtc ccagatcctt gagacgttgg atacccaagt ccacttcgtg
    23881 tttgacaaaa tcctctccga taatgccact agataaacta acaataccaa tagtagaaac
    23941 catatctcat cctcctagaa atagattgag cctattttat cacaaaatat aagagaaagc
    24001 tatgtgggat ttcagaagaa tacttttaat actcttcgaa aatctcttca aaccacgtca
    24061 gttttatttg caacctcaaa gctgtgcttt gagcaacctg cagctagctt cctagtttgc
    24121 tctttgattt tcattgagta taactcaaaa aagtaagtga aaaagcaacc gcacctgcag
    24181 ttgcttttat cgttttctta ttcttgatag cgtgttggtc ctgcacttgc actacggata
    24241 ttcaagcgtt tcttgagata gaagcgaagg gctaggaggg ctgctccaat aatagctagt
    24301 ggcaatggat ccagtactgg gttaaggcta gctggtagga agcttgttgc aaagaagaca
    24361 agcaaccaaa ggaacataga agctaggata actagtacag atttccagaa aggtggacgt
    24421 tgactgcaat ctatatctgg tccatagtat tggtaaacaa agtagtacat caagtagaag
    24481 gcaaatccac caaccagtcc aactaataga agagtaatca atccatagcc gaaagcttga
    24541 tctgccgcaa agaaggttgt gagggcgctg acaagggcaa agaggctagt gatgaaaaga
    24601 gctgagtcca taatcatcag ttttggatca tcattttctt ttggatgctc tttttcgtac
    24661 tgctctttga cagtgaagct atgagcccaa tgagttggtg cgccgtatag ggaacgggca
    24721 gttgtacctt tagattgctc ctcaaggatt tggggaatga cttcctcaaa aatagtctgg
    24781 atttcagcgt ctgttttccc atctttgatg aattgttggg tagcaatgtg gacaaactct
    24841 tggtttttct tagttaattt ttgtagatca atttgagaca taggaactcc tcttagaacc
    24901 attttttatg gatgagatag agagtgagcg agacactcat agcaaaggcg ataaagacga
    24961 ttaaccagaa ggcatttggc tctccgttta gggggatttc attatcctta aagttcatcc
    25021 cgtaggcaga aaagaccatg gttgggatgg acatgacgat ggtcacaagg gccaaggttt
    25081 tcatgatgtt gttctggttg ttagaaatga tagaggcaaa ggtctctgtc atagaatgca
    25141 agacgtttcc ataaatatct gccatctcga tggcctgttg ggtttcaatc agggtgtctt
    25201 caagcaggtc ttcgtcctca aggtatttct tgatattgct ggttgaactg gtcaatttct
    25261 taatcacgcg ctcatttgtt ttgagggagg ccttgaaata gacgatagtt ttttccaatt
    25321 ccatgagctc aatcaattct tcattacgag ttgattgatg cagttgactt tcgatttgtt
    25381 cactcttgcg gtcgattgaa cgaagggctg ttaggtaaag ctctgcattg cgataaagaa
    25441 tttgaaagat aaaacgtgaa cgcatgaagg tatagaaatt acgcaatcga cggttgataa
    25501 agacatcaag gacaggtagt ggttccaaac acgtagtgat aatggtttcc tcagtgatga
    25561 taataccaag cgggatggtt acgtagtagg tgcggttatt tctttcctcc gtgaccggca
    25621 cgtctacgat aatcagggta tactcgtctt caatggtaat acgagacatt tcttccgcat
    25681 cgagcggtgc tcgaaggtcg gcaatatcaa tatcgaaggt gttggcgatt tcgagtgatt
    25741 cattttgagt cggattgacg agattgatcc aagtacccgg ttcaagcgta tcgatctctt
    25801 taaattcagt tgttgtagag agaaaaactt gtttcatatc ccttatcctt tctcattttt
    25861 cagatttttt cacactgtac tattatacta caaaatcggc cttttgggta atagaatctc
    25921 actttttttg gtataatggt aagcaataat ggactagaaa gaacaaagat gcaagataaa
    25981 attgtcattc atggggcgcg tgcccataat ttaaaaaata ttgatgtgga gattccgcga
    26041 gacaagctgg ttgtcgtgac aggtttgtca ggttcaggga aatccagtct ggcctttgat
    26101 accctctatg cggagggaca acgtcgctat gtagagagtt tgtcagccta tgctcgtcaa
    26161 ttcttgggaa atatggagaa gccggatgtg gatgccattg atggtctcag cccagctatt
    26221 tctatcgacc agaaaacgac tagcaaaaac cctcgctcga cggtgggaac aacgactgaa
    26281 atcaatgact atctgcgtct cctctacgcg cgtgtgggga cgccttactg tatcaacgga
    26341 catggagcta tcaatgcttc ttctgtggag caaatcgtgg ataaggtctt ggaactgcca
    26401 gaacgtcagc gcctgcaaat cttagctcct gtcatccgca agaaaaaagg acagcacaag
    26461 agcgttgttg aaaagattca gaaagatggt tatgttcgtg tccgtgtgga tggggaagtc
    26521 tatgatgtga ccgaagtgcc agacttgtcc aagagcaagc aacacaatat cgatgtcgta
    26581 gttgaccgta ttgttatcaa ggagggtatc cgtagtcgtc tctttgattc cattgaggct
    26641 gcccttcgta ttgcagaagg ttatgtcatt atcgatacta tggacagttc tgagttactc
    26701 ttctctgagc attatgcttg tccagtttgt ggatttactg tcccagagtt agagcctcgt
    26761 ctcttctctt tcaatgctcc ttttggctct tgtagtgagt gtgatggctt gggcatcaag
    26821 ctggaggtgg atactgattt ggtagtacca gatgccagca aaaccttacg tgagggggcg
    26881 ctagcacctt ggaatcctat ttcatccaac tactatccaa atatgctaga gcaggccatg
    26941 acagcctttg gagtggagat ggataagcct tttgaggact tgtcagaaga agataagaac
    27001 ttgattcttt acggttcaga tggcaaggaa ttccatttcc actatgaaaa tgaatttggt
    27061 ggcgtgcgcg atatcgatat tccttttgag ggagttgtta ataatatcaa gcgtcgctat
    27121 catgaaacta atagtgacta cacccgcacc cagatgcgcc tttacatgaa tgagctgacc
    27181 tgcggaacct gtcacggcta tcgcctcaat gaccaggcct tgtctgtccg tgtgggtggc
    27241 gagaacgggc cacatatcgg agaaatttca gacctgtcta tcgcagacca cttggacttg
    27301 gtaagccagt tgactctatc tgaaaatgaa gccatcattg ctcgccctat tctcaaggaa
    27361 atcaaggacc gtttgacttt cctcaataac gtgggtctta actatctgac tctgtctcgt
    27421 tcagcaggaa ccctttcagg tggggaaagt cagcgcattc gtttggcaac ccagattggt
    27481 tccaacctat caggcgtcct ctatatccta gacgagccgt caatcggtct tcaccagagg
    27541 gacaatgacc gcctgattgc cagtctgaaa aagatgcgtg acttgggcaa tactcttatc
    27601 gtggtggaac acgacgaaga taccatgcgt gaagctgatt atctgattga cgttggtcct
    27661 ggtgccggtg tttttggtgg tgagattgtt gcagcaggta cacctaagca agtggctcgc
    27721 aacagcaagt ctatcacagg tcagtacttg tcaggcaaac gtgtcatccc agtaccagaa
    27781 gagcgccgtg tcggaaatgg tcgttttatt gaagtgatag gagcgcgtga gaacaacttg
    27841 caaaatgtca cggctcgctt tccactagga aaatttatcg cagtgacagg tgtgtcgggt
    27901 tcagggaaat cgactctaat caacagcatt ctcaaaaaag ccattgccca gaagctcaac
    27961 cgtaattcag acaaacctgg taagtttaag actattacag ggattgagca tgtcgaccgt
    28021 ttgattgata ttgaccagag ccctatcgga cggacgccga ggtctaaccc agctacctat
    28081 acaggagttt ttgacgatat acgtgacctt tttgctcaga caaatgaagc caagattcga
    28141 ggctacaaga aggggcgctt cagtttcaac gtcaagggag gtcgctgtga agcttgctca
    28201 ggtgacggga ttatcaagat tgagatgcac ttcttgccag atgtttatgt ggcttgtgaa
    28261 gtttgccacg gaacccgcta taacagtgaa accctagaag ttcactacaa ggaaaagaat
    28321 atttcgcagg tcttggatat gacggtcaac gatgcggtag aatttttcca acacattccg
    28381 aaaattcaac gcaaactcca gaccatcaag gatgtagggc taggctatgt gaccttggga
    28441 cagccagcta ccaccctttc tgggggagaa gcccaacgta tgaagttagc tagcgaactc
    28501 cacaaacgct cgacaggaaa atctttctac attctggatg agccgacgac agggcttcat
    28561 acagaggaca ttgctcgcct gctcaaggtt ttagctcgct ttgtagacga tggcaataca
    28621 gtcctcgtca tcgagcacaa tctggatgtt atcaagacgg cagaccatat cattgacttg
    28681 ggacctgagg gcggtgtcgg tggtggaacc atcatcgtaa caggaactcc agaagaagta
    28741 gctgccaacg aagccagcta tacaggacac tatttgaaag gaaagttaca tcatgaataa
    28801 acgcgtacaa gcatttctag ctaaaatgca agaaaaagaa ctagatggtg tcatcatcaa
    28861 caatcttaaa aacgtctatt atttgactgg tttttggggc tcaaacggaa cagtctttat
    28921 cagccgtgac cgtcaggtct tagtgacaga ctctcgctat atcatcgcag ctaagcaaga
    28981 aaccagtggt tttgagattg tggctgatcg tgatgaattg gctgtcattg caggaattgt
    29041 taaggacatg ggcttgactc gtatcggttt tgaagatgag atttcagtgt cttattacca
    29101 ccgtatgcag gcagcttttg caggtttgga cttgcttcca caaactcagt ttgtggaagg
    29161 tcttcgtatg attaaggatg aggcagagat tgcagcgatt cgcaaggctt gttctatctc
    29221 agaccaagct ttccgcgatg cgcttgactt tatcaaacca ggaaaaactg aaattgagat
    29281 tgccaacttc cttgatttcc gcatgcgtga gttgggagca tctggcttat cttttgatac
    29341 gatcctagct agcggtatca attcttctaa accccatgcc catccaatgc acaaaccagt
    29401 ggagttggga gaagccatta ccatggactt cggctgtctc tatgaccact atgtcagtga
    29461 tatgacccgg actatctatc tagggcatgt tagcgatgag caggcagaga tttacaatac
    29521 ggttctaaaa gctaaccaag ccttgattga ccaagctaag gcaggcttag gtttccgtga
    29581 ctttgacaaa atccctcgtg atattatcat tgaggcaggt tatggtgact actttactca
    29641 cggcattggc cacggtattg gtctggatat ccatgaggaa ccctacttta gccagacttc
    29701 tacagaaact attaagacag gtatggcctt gaccgatgaa ccaggtatct atatcgaagg
    29761 caaatatggc gttcgtatcg aggatgatat cctgattaca gagacaggtt gtgaattatt
    29821 gaccctagct ccaaaagagt tgatagtcat ttagttgttt gtcaagaaaa aagtacgaaa
    29881 atgacgaaaa aagtcaaaaa aaattaaaaa taggtcgcaa gtcggatgtt ttttatggta
    29941 taatagacta aactgatagt aacatgtagc gaaaggggta ggtacatgat taaaatttat
    30001 acagtctcaa gttgtactag ctgtaaaaaa gcaaaaacct ggctcaatgc ccaccagtta
    30061 agttataaag aacaaaacct tggtaaagaa ggaattacga gagaagaatt actggatatt
    30121 ctaaccaaaa cagataacgg aatagccagc atcgtttcgt ctaaaaatcg ctatgccaaa
    30181 gcccttggag tggatattga agatttgagt gtcaatgaag ttctcaatct gattatggaa
    30241 acaccgagaa ttttaaagag cccaatcctt gtagatgaaa aacgcctgca agttggctac
    30301 aaggaagacg atattcgtgc cttcctacca cgctctgtcc gtaatgtaga aaatgcagaa
    30361 gcacgtttgc gtgcagctct ataaacatca aggctgggag cacttcccag tcttattcta
    30421 ttttaatttc aaaaagaaag aagaaagaaa tgaaaaaaat agttcttgtt agtctagctt
    30481 tcctttttgt cctggttggt tgcggacaga aaaaagaaac tggaccagct acaaaaacag
    30541 aaaaagatac gcttcagtcg gcattgccag ttattgaaaa tgctgagaag aatacagttg
    30601 taactaagac tttggtcttg cccaagtcag atgatggtag ccagcagaca caaacaatta
    30661 cttacaaaga caagactttt ttgagtctaa ctatccaaca aaaacgtcca gtctctgatg
    30721 agttgaagac ttatattgac caacatggag tggaagaaac tcaaaaagct cttcttgaag
    30781 cggaggagaa ggataagtct atcattgaag ctcgtaaatt ggcaggtttc aaacttgaaa
    30841 caaaactatt gagcgcaacg gaacttcaaa caacgactag ttttgatttt caagttctgg
    30901 atgtcaagaa ggcttcccag ttggaacatc tgaagaatat tggtttggaa aatcttttga
    30961 aaaatgaacc aagcaaatat atttcagata gattggcaaa tggcgcgaca gaacaataga
    31021 aaatccaaat aaatagactg cctgaattgt agaaggtaag gaattatgct ataatggtac
    31081 tattctaagg aaagagggtg ttagaatggg atttactgaa gaaacagtac gttttaaatt
    31141 ggacgattcc aataaaaaag aaattagcga aactttgaca gatgtttatg cttcgttgaa
    31201 cgataagggt tacaacccaa ttaaccaaat cgtaggttac gtattgagtg gagaccctgc
    31261 ctacgttcct cgttataata atgcacgaaa tcaaatccgt aagtatgagc gtgatgaaat
    31321 cgttgaggaa ttggttcgct actacctaaa aggacaagga gtcgatctat aacctatgag
    31381 aattatggga ttggacgtcg gttcaaaaac ggtaggggtg gcgattagcg atccgcttgg
    31441 ttttacagct caagggcttg aaatcatcca gataaatgaa gaacaaggcc aatttggttt
    31501 tgaccgcgtt aaggaattgg ttgatactta caaggtggaa cgatttgtag tgggcttgcc
    31561 taaaaacatg aacaatacaa gtggaccgcg cgtagaagct agtcaagcct acggagcaaa
    31621 gctagaagag ttttttggtt taccagtaga ctatcaggat gaacgcttga caacagtggc
    31681 tgctgagcgc atgttgattg aacaagcaga tatcagtcgc aataagcgca agaaagtcat
    31741 tgataagtta gcagctcagc tgattttaca aaattattta gatagaaaat tttaatataa
    31801 aggagaggct atgtcacacg atcataacca cgaccacgaa gaacgtgaac taatcacact
    31861 agtagatgag caaggaaatg aaaccttgtt tgaaatcctt ttgacaattg atggaaaaga
    31921 agaatttggt aaaaactatg ttcttctagt accagttaac gcagaagaag acgaagacgg
    31981 acaagttgaa atccaagctt actcattcat tgaaaatgaa gatggaacag aaggcgaatt
    32041 gcaaccaatc ccagaagact cagaagacga atggaacatg attgaagaag tcttcaacag
    32101 ctttatggag gagtaaaaac gtccggggga cgtttttagc cctgcgctag aaattagaaa
    32161 cgcaggtagt ctgggagact gtatcagccc aactccagaa atggggaaga gttggaacgt
    32221 ccagtgaaca tttttacccc aactcttaga aattgagaag agttggaaca tctagtggat
    32281 gtttttagcc catattcaca ttcatagtag ctagttatta gctaaccagg taagaggtcg
    32341 ggacgaaaat cctggccttg tttttattag taaccatact ttaatgcgat aattgattgg
    32401 acttttgtca aggagatgtg tatgtttgaa gtagaagaat ggctccatag tcggattggt
    32461 ttgaattttc gatcaggttt gggccgaatg cagcaagcgg tggatttgtt aggaaatcct
    32521 gagcagtctt accctattat ccacgtaaca gggactaatg ggaaaggatc taccattgct
    32581 tttatgaggg aattatttat ggggcatggc aaaaaagttg cgacctttac ctcccctcat
    32641 atcgtctcta tcaatgaccg aatctgcatt aatgggcaac ctatagcaga cgcagacttt
    32701 atccgtttga ctgatcaggt caaggagatg gagaaaacgc ttctgcaaac tcctgcccag
    32761 ttgtcctttt ttgaattgct gaccttagtt gcttttcttt attttaggga gcaagaggtg
    32821 gatttggttt tattagaagt gggaattggt ggcttacttg acacgaccaa tgtggtaact
    32881 ggagatcttg ctgtcatcac ctccattggg cttgaccatc aagaaacctt gggtgatagt
    32941 ctagaagcaa ttgcagagca gaaagctggt attttcaagg ctggtaagaa ggcagtgatt
    33001 gcgaaattgc ctccagaagc taggcttgtc tgtcagaaaa aagccgaatc tttagctgtt
    33061 aacctttatc aggcaggtca agatttttta atgctgaatg gtgatttttc aagctcttta
    33121 ctaaatcttt cacagctgaa cataggctta gaaggagtct atcagcagga gaatgcagcc
    33181 ttggcgttgc aaacttttct tctttttatg ggagaaggaa aggaagctgt tgatgaacag
    33241 gctgtaagaa aggccttgga acagacccat tgggctggtc gcttggagcg tattcgccca
    33301 cagatttact tggatggtgc tcataacctc cctgccttga ctcgcttggt tgagtttatc
    33361 aaagaaaaag agcaggaagg ctatcgacct caaatccttt ttggagcctt gaaacggaag
    33421 gattatcaag ggatgttagg ttacctgact gaaaaattgc ctcaggtgga actcaaggtg
    33481 accggctttg actatcaggg ggctttggac gaaagggatg tgacaggtta cgatatagtt
    33541 tcttcttacc gagaatttat cagcgatttt gaagaaagag cagatgctca ggatttgctg
    33601 ttcgttacag ggtctctcta ttttatctca gaagtacggg gctacctgct ggaccgtgag
    33661 cagataaatt gacccccttg tttgaacttg ttatactagg ggaattgctg gttagagaaa
    33721 atttctctaa attggtagca gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt
    33781 ctcttgcttc tctagcaagt tttagtctct tagtagcttg ttcacaaaga gctcaacagg
    33841 ttcaacagcc tgttgctcag cagcaggtcc aacagcctgc tcaacagaat accaatactg
    33901 caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca aaaccaacct gttgctcaac
    33961 cgaccgatat tgatgggact tatactggtc aggatgacgg agaccgtatc actttagtgg
    34021 taactggaac gactggtaca tggactgagc tcgaatctga cggggatcag aaagtcaaac
    34081 aggttacatt tgattcagca aatcaacgca tgattattgg cgatgatgtc aaaatttaca
    34141 ctgtaaacgg taatcaaatc gtcgtagatg atatggatag agacccatcg gaccaaatcg
    34201 ttttaactaa ataagactat gcaagtagaa agggctggat ttttcagcct ttttactttt
    34261 acctagaaaa taatcataaa tacttgcctt ctaccgaata cctgcgttat actagtatca
    34321 attacttgtt tttagcattc aaactatcaa ggaggggata tgaaatatag gaaatttcaa
    34381 ttattgatgt ccaagtatgg ctttagtctt tcgattatgc tacttgaact ttgtcttgtt
    34441 tttggtctct ttctttattt aggacgcatg gctcccattt tatggattac tgtcctcatt
    34501 ctactgagta tcatcacaat catttcgata gtcaaccgta atacgactcc tgagaataag
    34561 gtaacctggt tgttagtagc ctttgtgcca gtatttggtc ccttgctcta tctgatgttt
    34621 ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga agaagctagg ctctatgcat
    34681 ttccaagaag caaatagcca gctactaaaa gagaaattaa aagaaagtga caaggcagct
    34741 tatggagtca tcaagtcctt attgagtatg gataccaatg ctgacatcta tgatcaaact
    34801 gcctctacat tttttcctaa cggagaagct atgtggaaaa agatggtaga agatcttaaa
    34861 aaggctgaga aatttatttt cttggaatat tacattatag aagaaggttt gatgtggaat
    34921 cgcatactag atatactaga gcaaaaggta gctcagggtg tagaggttaa gatgctctat
    34981 gatgatatcg gctgtatggc tactttaaca ggagattatg cacatcgact tcgtcagctg
    35041 ggcatcgagg cccataaatt caataaagtt attcctcgtt tgacagtggc ttataataac
    35101 agagatcata gaaaaatatt gattgttgat ggtcagatag cctatactgg tggggtcaat
    35161 ctggcagatg agtacattaa ccacgtcgag agatttggtt attggaagga tagtggaatt
    35221 cgcttagacg gactagcagt aaaagctctg acacgcttat ttttgaccac ttggtacatt
    35281 aatcgaggag aaattagtga ttttgatcaa tatcatttag aaaatcattc tatcccgagt
    35341 gacggtttaa ccattccata cggaagtgga cccaagccaa tttttcgagc gcaggtaggg
    35401 aaaaaagttt atcagagttt aatcaatcaa gcaacagaat cggtctatat tacgacacct
    35461 tatttgatta tagattatga tttaacagag acaatcaaaa atgcagctat gagaggggtc
    35521 gatgttcgaa ttatcacccc ttacatacca gataagaagt tcattcagtt agtcacgaga
    35581 ggagcttatc ccgaccttct ttctgctggt gttcggattt atgagtatag tccaggtttt
    35641 attcatagta agcagatgtt ggtagacgaa gattttgcgg tggtggggac aatcaatctc
    35701 gactaccgga gcttggtaca ccattatgaa aatgcagtct tactctataa aactccttct
    35761 ataagggaaa tcgcccgaga ttttcgaaat atatttgcag attctcagga agtctatcct
    35821 cattctatca aaacgagctg gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc
    35881 cctatcttat aagaaatcta gaacttagga tacaaaccag acctattttt cttgtctttt
    35941 acgaatagaa agaaagagga ggggaattca tgctaactaa ggaagaagtg aatgatatga
    36001 ttgagttcaa acagacacat ttacaccgct ttcgggaaat tgaaaccttt gtgaaactat
    36061 gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc taggactttt tgatatacta
    36121 agacagataa attgaacagg agaaacgata tgagtgtgta tggtagagta gaagaagttc
    36181 ataaggagaa tcgtgaacct ctagaatacc aaatcgaaca agaatcgcat catcgtgaat
    36241 caagtcgtct tcctttggtg aaaattttac tatggagtac gcttgtaacg gggataactc
    36301 taggagtgcc gctattactc gacttaatga gtgcacaaga agtgcaggat ttttatgcag
    36361 gttgggcgct tcatcagaca gggaagattt acagcgacta ttatggaagt caaggtttgc
    36421 tttattattt gctgacttac gtgagtcagg gcggattttt ctttgccatc tttgagtggt
    36481 tagccttggt agcaggcgga tttttccttt ttagatcagc ggacaccttg acagagcaag
    36541 gagaccaagc tggacatctg gtgactattt tttacatgct agttacaggt cttgcttttg
    36601 gtggaggcta tgcgactctt ttagcgcttc ctttcttatt cgcagccttt agtttagttg
    36661 cggcttacct aagcaatcca agccatgata agggatttgt acggattggg ctagctttgg
    36721 caggcggatt tttctttgct cccttatcat cgctcctgtt tattgctgta gtgagtttag
    36781 gcttgttggt ctttaacctt gggcatagac gttttgtgca tgggttttat cagtttcttg
    36841 cagtggcttt aggtttttca cttgtctttt atccaactgc ctactatagt gctgcaacag
    36901 gaagttttgg ggatgcgatt agtggtattc gttatcctat tgacagtatt cgctttgatt
    36961 ttacttctaa aattttagag aatatgtttt tttatggctt gttgtccctt ggtttgggat
    37021 ttgtggtttg tatcttttta ggtctctttc aatccaaacc ctttaaacta tacgtcattt
    37081 cggttcctgc aagtcttgtg gtaattttag gtttgatttt acttttcttt tcacaagagc
    37141 ctctgcacgc ttcttatttg atggtcgtct tccctgtttt cctactttta ttggtaacca
    37201 atattaagag tcaacagagg gggcgtagtg ctagaagaag ccgaagagaa acgccagtta
    37261 gcctatggag tcgtttcttc aaaggaaatc tatatctgct agtttttggg tttgtctatc
    37321 ttttgtctgt tccttttttg atgaagtttg tcctttatcc agtaccttat caagaacgta
    37381 atcgtcttgc tgatttggta aaagaggaga caaatacgga agatgctatc tatgcatggg
    37441 atgatactgc gactctttat cgtaagagtg agcgcttgtc gccatcggcg attttgtccc
    37501 cgttgcacta tacagcaact gaggaaaatc gtaataagtt acttaatgac ttgaaagaaa
    37561 aacaacctaa ggtgattgtg gtaaatgaca aggtggtagt ctggtctgaa gtggaaacac
    37621 tcttaaaaga aaattaccaa caagtaaaga ctgattactc agagtttaaa gtctataaaa
    37681 ttaaataacc aaat
//
LOCUS       DAVMAF010000011        36330 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMAF010000011 DAVMAF010000000
VERSION     DAVMAF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.011"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            5..75
                     /locus_tag="VYO97_000537"
     tRNA            5..75
                     /locus_tag="VYO97_000537"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VYO97_000538"
     tRNA            110..183
                     /locus_tag="VYO97_000538"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            267..356
                     /locus_tag="VYO97_000539"
     tRNA            267..356
                     /locus_tag="VYO97_000539"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Ser,seq:tga)
     gene            367..440
                     /locus_tag="VYO97_000540"
     tRNA            367..440
                     /locus_tag="VYO97_000540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Met,seq:cat)
     gene            444..516
                     /locus_tag="VYO97_000541"
     tRNA            444..516
                     /locus_tag="VYO97_000541"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:477..479,aa:Phe,seq:gaa)
     gene            529..609
                     /locus_tag="VYO97_000542"
     tRNA            529..609
                     /locus_tag="VYO97_000542"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:563..565,aa:Tyr,seq:gta)
     gene            614..684
                     /locus_tag="VYO97_000543"
     tRNA            614..684
                     /locus_tag="VYO97_000543"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Trp,seq:cca)
     gene            699..771
                     /locus_tag="VYO97_000544"
     tRNA            699..771
                     /locus_tag="VYO97_000544"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:732..734,aa:His,seq:gtg)
     gene            782..853
                     /locus_tag="VYO97_000545"
     tRNA            782..853
                     /locus_tag="VYO97_000545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Gln,seq:ttg)
     gene            863..946
                     /locus_tag="VYO97_000546"
     tRNA            863..946
                     /locus_tag="VYO97_000546"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Leu,seq:caa)
     gene            1053..2330
                     /locus_tag="VYO97_000547"
     CDS             1053..2330
                     /locus_tag="VYO97_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1753396.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFSLNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2406..3890
                     /locus_tag="VYO97_000548"
     CDS             2406..3890
                     /locus_tag="VYO97_000548"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1753397.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3951..5231
                     /locus_tag="VYO97_000549"
     CDS             3951..5231
                     /locus_tag="VYO97_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1753398.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGITGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            5235..5855
                     /locus_tag="VYO97_000550"
     CDS             5235..5855
                     /locus_tag="VYO97_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1753399.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6021..7163
                     /locus_tag="VYO97_000551"
     CDS             6021..7163
                     /locus_tag="VYO97_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1753400.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVFWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASNSEVAETV
                     TAAEEVALSTDSTTPEGQPAETTSPVEEATPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7569..7907
                     /locus_tag="VYO97_000552"
     CDS             7569..7907
                     /locus_tag="VYO97_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1753401.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7918..8276
                     /locus_tag="VYO97_000553"
                     /pseudo
     CDS             7918..8276
                     /locus_tag="VYO97_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            8383..9001
                     /locus_tag="VYO97_000554"
                     /pseudo
     CDS             8383..9001
                     /locus_tag="VYO97_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9029..9886)
                     /locus_tag="VYO97_000555"
     CDS             complement(9029..9886)
                     /locus_tag="VYO97_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1753402.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10016..11158
                     /gene="tgt"
                     /locus_tag="VYO97_000556"
     CDS             10016..11158
                     /gene="tgt"
                     /locus_tag="VYO97_000556"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1753403.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11259..13076
                     /locus_tag="VYO97_000557"
     CDS             11259..13076
                     /locus_tag="VYO97_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1753404.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13229..14380
                     /gene="nagA"
                     /locus_tag="VYO97_000558"
     CDS             13229..14380
                     /gene="nagA"
                     /locus_tag="VYO97_000558"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1753405.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPYTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14543..15601
                     /locus_tag="VYO97_000559"
     CDS             14543..15601
                     /locus_tag="VYO97_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1753406.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15752..16117
                     /locus_tag="VYO97_000560"
     CDS             15752..16117
                     /locus_tag="VYO97_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1753407.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16193..17134
                     /locus_tag="VYO97_000561"
     CDS             16193..17134
                     /locus_tag="VYO97_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1753408.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     KDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17082..18098
                     /locus_tag="VYO97_000562"
     CDS             17082..18098
                     /locus_tag="VYO97_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1753409.1"
                     /translation="MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRTGLSQGKSFSEIMASLGFSSAIVTQLSLAEVHGNLHLSLRKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGFVSVGVLLALTFYKRSSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQIMQEQGSQLFKEIGQDLAQSLQNGREFSQTIGTYPFFKKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18100..18180
                     /locus_tag="VYO97_000563"
                     /pseudo
     CDS             18100..18180
                     /locus_tag="VYO97_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(18180..19625)
                     /locus_tag="VYO97_000564"
     CDS             complement(18180..19625)
                     /locus_tag="VYO97_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1753410.1"
                     /translation="MITTNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCDIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHRDTGTITINPAQALAIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEASVLKEISKLQDNADYLDKIFSGDNDETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRATLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYEGQKVLVRGLINKVQVTAEDIVIKWKI"
     gene            complement(19935..20369)
                     /locus_tag="VYO97_000565"
     CDS             complement(19935..20369)
                     /locus_tag="VYO97_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV1753411.1"
                     /translation="MKFQQGEVYLINFPQKGGNEFYGKHYAIILTTPDKVDGTLLVAP
                     LTGKKSGKKYRGGITIENSKYQNTPSKPKAYAYVRKIQEIDKRKIVYKTKKKTDSDGQ
                     VMLDAAGKELYDKVYRPAYKLDTNDHKKLLDKIKEVLGLDLY"
     gene            complement(20555..20755)
                     /locus_tag="VYO97_000566"
     CDS             complement(20555..20755)
                     /locus_tag="VYO97_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753412.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            complement(20757..21545)
                     /locus_tag="VYO97_000567"
     CDS             complement(20757..21545)
                     /locus_tag="VYO97_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753413.1"
                     /translation="MKLGELLKSYRTEHKLSMDAFCELSDLTKGYISMLEKNEHPKSK
                     KPIVPSYDTIEKIAKGMQISTDDLINMLDDEQEIQINATPAVLSKSQIQSIYDELEPN
                     GQRKVVTYAEKLRDEQEKRKKAKINEVSENIIRLDDYRQTTYRRVTGVVSAGSGSIQD
                     DDLDMEVSFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIGIFQVD
                     GANYVKKLRQGYLESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            21702..21977
                     /locus_tag="VYO97_000568"
     CDS             21702..21977
                     /locus_tag="VYO97_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753414.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSKLEAFCTNCRQF
                     AFGWTELADEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            22052..22219
                     /locus_tag="VYO97_000569"
     CDS             22052..22219
                     /locus_tag="VYO97_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753415.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(22210..22659)
                     /locus_tag="VYO97_000570"
     CDS             complement(22210..22659)
                     /locus_tag="VYO97_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753416.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNMSTLSHFANKQQPSDLQKQFVKKLSKKNHK"
     gene            22858..23028
                     /locus_tag="VYO97_000571"
     CDS             22858..23028
                     /locus_tag="VYO97_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753417.1"
                     /translation="MKEKLNEFLKFRSQFTKREWIEINQVVEARLNEKADQLKLDDSD
                     VEIISKRLGRSI"
     gene            complement(23253..23459)
                     /locus_tag="VYO97_000572"
     CDS             complement(23253..23459)
                     /locus_tag="VYO97_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753418.1"
                     /translation="MTDHQLETSLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVRIARLKPDQITFLIK"
     gene            23532..23720
                     /locus_tag="VYO97_000573"
     CDS             23532..23720
                     /locus_tag="VYO97_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753419.1"
                     /translation="MQGERLRKWREKEKMSQEELAEKSNVSRTTIHLIESGQSSTVKI
                     RTLQKLAVVFNKQVKDFF"
     gene            23884..24225
                     /locus_tag="VYO97_000574"
     CDS             23884..24225
                     /locus_tag="VYO97_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1753420.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVNRKTIDKYFKIFEEAGYLRK
                     IRKKPPGNGGSHIFRFFSDVKISDFQFDIMKQRLNLSIKKASMNYNSDIPKSEMSESE
                     MSESEMSDFGH"
     gene            complement(24509..24700)
                     /locus_tag="VYO97_000575"
     CDS             complement(24509..24700)
                     /locus_tag="VYO97_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753421.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            24819..24962
                     /locus_tag="VYO97_000576"
     CDS             24819..24962
                     /locus_tag="VYO97_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753422.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            25027..25188
                     /locus_tag="VYO97_000577"
     CDS             25027..25188
                     /locus_tag="VYO97_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753423.1"
                     /translation="MKRKKEQWKPRIVNIMADGSQVDDLTGYVIPAGHSYYDIILGMN
                     KQSNEEGVA"
     gene            25188..25391
                     /locus_tag="VYO97_000578"
     CDS             25188..25391
                     /locus_tag="VYO97_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753424.1"
                     /translation="MKLLTKLKLRLEVVLKAVNLDWREVAVELMIDLFEERKRRFAFE
                     QENYDLKQQLAIYKEKEQGEQYV"
     gene            25384..25638
                     /locus_tag="VYO97_000579"
     CDS             25384..25638
                     /locus_tag="VYO97_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753425.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPETERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEDVEQEDD"
     gene            25631..25792
                     /locus_tag="VYO97_000580"
     CDS             25631..25792
                     /locus_tag="VYO97_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753426.1"
                     /translation="MTRIELENRVWLLANHEEKKRIAGSWANVKDQICEASSGTRKGV
                     CSCLITTET"
     gene            25895..26125
                     /locus_tag="VYO97_000581"
     CDS             25895..26125
                     /locus_tag="VYO97_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753427.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            26137..26889
                     /gene="bet"
                     /locus_tag="VYO97_000582"
     CDS             26137..26889
                     /gene="bet"
                     /locus_tag="VYO97_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV1753428.1"
                     /translation="MTNNQLATQTKRNITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYTNRNGGKEFSLIVSKEAFLKRAAQCKDYEGFEAGVVV
                     VDSEGVMHERKGAIMLPEDTLIGGWARVHRKNFKVPVEIFVSREEYDKKQSTWNTMPA
                     TMIRKVALVNALREAFPEDLGNMYTEDDGGETFDRIKDVTPQESREDVVARKMAQIEQ
                     FNKEQEANHADPEPAKTEEPIQGELLDGELEY"
     gene            26899..27987
                     /locus_tag="VYO97_000583"
     CDS             26899..27987
                     /locus_tag="VYO97_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1753429.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTASTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKAGDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFEQ
                     IKADYESEKQKEEIRQAQERAERESQELLAAQQTKQQERAPKSTGTPNFDPEAGEILD
                     GEQIPQNEQNSLRGAENDLKRYTQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQV
                     SGYQRIEPLTQAELNEQWGGNHGHQKNI"
     gene            27965..28288
                     /locus_tag="VYO97_000584"
     CDS             27965..28288
                     /locus_tag="VYO97_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753430.1"
                     /translation="MDIRKISDSVAIYSDGKKLHVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            28281..28616
                     /locus_tag="VYO97_000585"
     CDS             28281..28616
                     /locus_tag="VYO97_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV1753431.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            28711..29127
                     /gene="ssb"
                     /locus_tag="VYO97_000586"
     CDS             28711..29127
                     /gene="ssb"
                     /locus_tag="VYO97_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1753432.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            29137..29643
                     /locus_tag="VYO97_000587"
     CDS             29137..29643
                     /locus_tag="VYO97_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1753433.1"
                     /translation="MSKIKILDACCGSRMFWFDKNESHTIFMDIRQETFEIHDKKVNV
                     DPDIIGDFRDMPFEDNTFNLVVFDPPHLKWAGPNSIMKAQYGQLDKVTWSEDLAKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKKLLEVIPFKPLFGQQRGTTHWLTFVKFEEDKNGV
                     DRLGGLET"
     gene            29609..29818
                     /locus_tag="VYO97_000588"
     CDS             29609..29818
                     /locus_tag="VYO97_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753434.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     LDIKRDCLRLGVPFEDVYPLQTTLF"
     gene            29832..30161
                     /locus_tag="VYO97_000589"
     CDS             29832..30161
                     /locus_tag="VYO97_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753435.1"
                     /translation="MASKINATERIAIIIEKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            30158..30589
                     /locus_tag="VYO97_000590"
     CDS             30158..30589
                     /locus_tag="VYO97_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV1753436.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRTRLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            30605..30787
                     /locus_tag="VYO97_000591"
     CDS             30605..30787
                     /locus_tag="VYO97_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753437.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            30812..31129
                     /locus_tag="VYO97_000592"
     CDS             30812..31129
                     /locus_tag="VYO97_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753438.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYELGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGN"
     gene            31131..31802
                     /locus_tag="VYO97_000593"
     CDS             31131..31802
                     /locus_tag="VYO97_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1753439.1"
                     /translation="MNVQRLIEKYKKLEGVWDAEGAELARQIFLEDLKQLDESEIGYA
                     DEAPRYVKNILARLRELPLHDREVWLKAIMSEFEQDFSRAKWREGYEQGKIEGMVERE
                     KVKVPRFVAEWIEEARKACKDVVELFEFDFTNDEVRKWFMQERPFDLVARAWLDGYEV
                     EEEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEEAGLGWVFDCEGVE
                     IEEVE"
     gene            31802..31963
                     /locus_tag="VYO97_000594"
     CDS             31802..31963
                     /locus_tag="VYO97_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753440.1"
                     /translation="MSFTLNSTIGDLVLAIGEIIVGSDGKTTTAILEIPDQSFYLEIE
                     LKLKEEVIN"
     gene            32107..32523
                     /locus_tag="VYO97_000595"
     CDS             32107..32523
                     /locus_tag="VYO97_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753441.1"
                     /translation="MIPKFRVWHYELGRLMSVKCMFFQDSEIEEFELNDALMNDYITA
                     YPDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIGIVKQLEG
                     SWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            32520..32891
                     /locus_tag="VYO97_000596"
     CDS             32520..32891
                     /locus_tag="VYO97_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753442.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFVR"
     gene            32961..33383
                     /locus_tag="VYO97_000597"
     CDS             32961..33383
                     /locus_tag="VYO97_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1753443.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRLINKLKNPKSRSILRVTYITK
                     MYVDDICDKMEISRTTFYTWRNMAISELNEVLERMELN"
     gene            33469..33551
                     /locus_tag="VYO97_000598"
     tRNA            33469..33551
                     /locus_tag="VYO97_000598"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33500..33502,aa:Tyr,seq:gta)
     gene            33851..34345
                     /locus_tag="VYO97_000599"
     CDS             33851..34345
                     /locus_tag="VYO97_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1753444.1"
                     /translation="MDIGLNQRQKMFASEYLRTGNVYQSAIFAGYSEAYAKTTASKLL
                     ENASVKTFIQTETEKMHDENILSAKEALSILSDIARGQRLEEVLMMNPVTGEVDRVTK
                     KADNNTVIKAIAEILKRYPTAKQAEKLELEIEKLKSQIGVDNEQDDKLIDFAKALRGA
                     FDDK"
     gene            34335..35549
                     /locus_tag="VYO97_000600"
     CDS             34335..35549
                     /locus_tag="VYO97_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1753445.1"
                     /translation="MTNKFTKRQEEVLTRVLNDDFFICGLHGAKRSGKTVLNNMVFMN
                     EIARVRETADRLNIDEPMYILAGTSSTSIQNNIIQELYNMFDIEPKYDKHGAFTLCGV
                     KVVQVYTGSISGLKRARGFTAFGAYVNEASLANEQVFKEIISRCSGEGARIVWDSNPD
                     IPTHWLRRDYINSGDDMIIDFHFKLDDNTFMSDRYRENIKNATPAGVFYDRDILGLWV
                     TGEGVVYRDFSENMVVDNVPEDITKVYAGVDWGYEHFGSIVVIGETSGGSAYLLEEHA
                     HQYKEIDFWVDLAKNIKERYGNITFWADSARPEHVARFQREQLRTFNANKAVLSGIEE
                     VAKLMKAGRFFVVSNKVSKFKDEVYQYIWNEKTGEPVKENDDVLDAVRYAIYSQHSQP
                     KATVRRRSDYGL"
     gene            35561..>36330
                     /locus_tag="VYO97_000601"
     CDS             35561..>36330
                     /locus_tag="VYO97_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1753446.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTESSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVES"
BASE COUNT        11141 a         6441 c         8179 g        10569 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaaac tattttggag gattacccaa gtccggctga agggaacggt
      301 cttgaaaacc gtcaggcgtg taaaagcgtg cgtgggttcg aatcccacat cctcctttta
      361 tattaacgcg ggatggagca gctcggtagc tcgtcgggct cataacccga aggtcgtagg
      421 ttcaaatcct gctcccgcaa taaggctcgg tagctcagtt ggtagagcaa tggattgaag
      481 ctccatgtgt cggcggttcg attccgtctc gcgccattta tatattttgg aagggtagcg
      541 aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg gttcgggggt tcgaatccct
      601 ccccttccat tttacgggca tagtttaaag gtagaactaa ggtctccaaa accttcagtg
      661 tgggttcaat tcctactgcc cgtgttaata gaattatggc gggtgtggtg aagtggttaa
      721 cacaccagat tgtggctctg gcatgcgtgg gttcgatccc catcactcgc ctattttata
      781 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      841 ccagctaccc cagttactat ttgccggcgt ggcggaattg gcagacgcgc tggactcaaa
      901 atccagtgtc cgcaaggacg tgccggttcg accccggccg ccggtatagt atagtgttag
      961 gaacgttgtt attcttcgtt ccttttttat attatttttg gtataattat agttattcaa
     1021 attttgttta gattgagaaa gtgtagggta gtatgtcttg ttctatcgat ttattaaaac
     1081 atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt tgtcggaatt gagttggagt
     1141 atcctgttgc aagtttagaa ggggatgcta cagatgttga agttataaag gatctatttc
     1201 attatttagt ttctactttg gatctcaccg tagcaaaggt agatgatttt ggcaatctga
     1261 tccagttagt agatccgata agtcaggatg ctattttatt tgaagtttcc tatacaacga
     1321 ttgagtttgc atttggtaag gctgaaacga ttcaagaggt cgaaaatcgt ttcaataatt
     1381 atatgaatgt aattcagaga aagttagctg aatcaaatca tgctattgtt ggctgtggta
     1441 tccatcccaa ctgggataaa aatgagaatt gtccagtggc ttatccacgc tatcagatgt
     1501 tgatggatta tttgaatttg agtagaaata ttattaaatc agatttacat catttccctg
     1561 aatatggtac ttttatctgt gggagccagg ttcagctgga tatttcaaaa accaactact
     1621 tacgggtgat taatgctttt actcaaattg aagcggctaa ggcttattta tttgcaaact
     1681 ctgaattttc gggtgcggat tgggatacga aaatttcaag ggatattttc tgggaagaat
     1741 ctatgcatgg tatctatcca gagaatgttg gggtcaatgc tagactcctt aatgatgaag
     1801 ctgatttttt tgactatcta aatcattctg cgatttttac tgcggaacgt gatgggcaga
     1861 cctattattt ttatcctatt caggctgggg actatttggc tacgcctgaa atccaagcat
     1921 tttctctgaa tggggatgag gttattattt ccccccaaga gaaggatttt gaaactcatc
     1981 gtagttacca gtaccaagac ttaacgactc gaggaacagt tgagtttcgt agtgtgtgta
     2041 cacagccact tgatagaact tttgcttctg cagcttttca cttgggatta ttggttaatt
     2101 tagacaagtt agaagcttac ttagaaacag cacctttctt taaagtattt ggttatgatt
     2161 acaagtcttt aaggagacaa ttttctaaga aaaatcttac agatgaggaa gaaactacga
     2221 ttattgaatt ttccaaagac ttactcctac tagctgagga gggactagtg gtgagaaata
     2281 aggaagaaat gacctattta cagcctttga gagaagaatt gagcctataa tttctcttat
     2341 aaagggagaa ttttctgaaa aatcatgata taatggacga gactatagat aaaggataga
     2401 gagtaatgac attagtttat caatcaacgc gtgatgccaa caatacagta actgccagcc
     2461 aagcaatttt gcaaggttta gcgacggacg gcggtttgtt tacaccggtt acttatccaa
     2521 aggtagattt gaactttgac aaattgaaag atgcttctta ccaggaagtt gctaagctag
     2581 ttttgtcagc atttttagat gactttacag ttgaggagtt ggactactgt atcaacaatg
     2641 cctacgatag caaatttgat actccagcta ttgcaccatt agtgaaatta gatgggcaat
     2701 acaatttgga acttttccat ggttcaacga ttgcctttaa ggatatggcc ttgtctattt
     2761 tgccatactt tatgacgact gctgctaaga aacatggttt ggagaacaag attgttatct
     2821 tgacagcgac atctggtgac acggggaaag ctgctatggc ggggtttgcg gatgtgcctg
     2881 gtactgagat tatcgtcttt tatccaaagg atggtgtcag caagattcaa gagttacaaa
     2941 tgaccactca gactggcgac aatactcatg ttattgctat tgatggtaac tttgacgatg
     3001 cgcaaacaaa tgtgaagcac atgtttaacg atgtggctct tcgtgaaaaa ttgactacca
     3061 acaagttgca attttcatca gctaactcta tgaacattgg tcgtctggtg ccacaaattg
     3121 tttattatgt ttatgcctac gctcaattgg ttaagactgg tgaaattgta gctggtgaaa
     3181 agattaactt cacagtacca acaggaaact ttggaaatat cttggctgcc ttttatgcca
     3241 aacaaatcgg cctaccagtt ggcaagctaa tctgtgcttc aaatgacaac aatgttttga
     3301 cagacttctt taaaacacgt gtctatgaca aaaaacgtga gtttaaggta acaaccagcc
     3361 catctatgga tatcttggta tcttcaaact tggagcgctt gattttccat cttttgggaa
     3421 ataatgctga aaagacagct gaacttatga atgccttgaa cacgcaagga caatataagt
     3481 tgacagactt tgatgcagag attttggacc tctttgcagc tgaatatgcg actgaggaag
     3541 aaacagcggc agaaatcaag cgtgtttatg agtcagattc ttatattgag gatccacata
     3601 cggcggttgc ctcagcagtt tatagaaaat accaagcggt tactgatgat gcgactaaga
     3661 cagtgattgc ttcaacagct agtccataca agttcccagt agttgcagta gaagctgtaa
     3721 ctggaaaagc aggtttaaca gactttgaag ccttggctca attacatgaa atctcaggcg
     3781 ttgcagtgcc accagcagtt gatgggcttg aaatagctcc aattcgtcac aagacaacag
     3841 tggcagctgc tgacatgcaa gcagcggttg aggcttattt aggactttaa gacagaggga
     3901 gcaaactcgg ttgggaaacc aactgagttt cttttcatca ggaggagaga ttgtttaaga
     3961 aaaataaaga cattcttaat attgcattgc cagctatggg tgaaaacttt ttgcagatgc
     4021 taatgggaat ggtggacagt tatttggttg ctcatttagg attgatagct atttcagggg
     4081 tttcagtagc tggtaatatt atcaccattt atcaggcgat cttcatcgct ctgggagctg
     4141 ctatttccag tgttatttca aaaagcatag ggcagaaaga ccagtcgaag ttggcctatc
     4201 atgtgactga ggcgttgaag attaccttac tattaagttt ccttttagga tttttgtcca
     4261 tcttcgctgg gaaagagatg ataggacttt tggggacgga gagggatgta gctgagagtg
     4321 gtggactgta tctatctttg gtaggcggat cgattgttct cttaggttta atgactagtt
     4381 tgggagcctt gattcgtgca acgcataatc cacgtctgcc tctctatgtt agttttttat
     4441 ccaatgcctt gaatattctt ttttcaagtc tagctatttt tgttctggat atggggataa
     4501 ctggtgttgc ttgggggaca attctgtctc gtttggttgg tcttgtgatt ttgtggtcac
     4561 aattaaaact gccttatggg aagctaactt ttggtttaga taaggaactg ttgaccttgg
     4621 ctttaccagc agctggagag cgacttatga tgagggctgg agatgtagtg atcattgcct
     4681 tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc aatcggagaa gtcttgaccc
     4741 agtttaacta tatgcctgcc tttggcgtcg ctacggcaac ggtcatgctg ttggcccgag
     4801 cagttggaga ggatgattgg aaaagagttg ctagtttgag taaacaaacc ttttggcttt
     4861 ctctgttcct catgttaccc ctgtccttta gtatatatgt cttgggtgta ccattaactc
     4921 atctctatac gactgattct ctagcggttg aggctagtgt tctagtgaca ctgttttcac
     4981 tacttggaac ccctatgacg acaggaacag tcatctatac ggcagtctgg cagggattag
     5041 gaaatgcacg cctccctttt tatgcgacaa gtataggcat gtggtgtatc cgcattggaa
     5101 caggatatct gatggggatt gtgcttggtt ggggcttacc tggtatttgg gcagggtctc
     5161 tcttggataa tggttttcgc tggttatttc tacgctatcg ttaccagcgc tatatgatct
     5221 tgaaaggata ggaaatgcaa aaaacagctt ttatttggga tttagacggg actttattgg
     5281 actcttacga agcgatttta tcagggattg aggagacttt tgctcagttt tctattcctt
     5341 atgataagga gaaggtgaga gagtttatct tcaagtattc ggtgcaagat ttgcttgtgc
     5401 gggtggcaga agatagaaat ctggatgttg aggtgctaaa tcaggtgcgt gcccagagtc
     5461 tggctgagaa gaatgctcag gtagttttga tgccaggtgc gcgtgaggtg ctagcttggg
     5521 cagacgaatc aggaattcag cagtttatat atactcataa ggggaacaac gcttttacca
     5581 ttctcaagga cttgggggtg gaatcctatt ttacagagat tttaaccagt cagagtggct
     5641 ttgtgcggaa gccaagtcca gaagcggcta cctatctgct agataagtat cagttgaatt
     5701 ctgataatac ttattatata ggggatcgga ctctggatgt ggaatttgcc cagaatagtg
     5761 ggattcaaag tatcaacttt ttagagtcta cttatgaagg gaatcacagg attcaagcgt
     5821 tagcagatat ttcccgtatt tttgagacta agtgataaaa agattgtgtc agttttgtga
     5881 cagagaccta acaaactatt tcaagtaatc gagtttgtta caaggaatag acagttctgt
     5941 taaataggcc cgagagggct ttttttctac attttttgtg ttatcataga caggtactca
     6001 tttgaaagga atttgaaaga atgaagaaaa gaatgttatt agcgtcaaca gtggccttgt
     6061 catttgcccc agtattggca actcaagcag aagaagtttt ttggactgca cgtagtgttg
     6121 agcaaatcca aaacgatttg actaaaacag acaacaaaac aagttataca gtacagtatg
     6181 gtgatacttt gagcaccatt gcagaagcct tgggtgtaga tgtcacagtg cttgcgaatc
     6241 tgaacaaaat cactaatatg gacttgattt tcccagaaac tgttttgaca acgactgtca
     6301 atgaagcaga agaagtaacg gaagttgaaa tccaaacacc tcaagcagat tctagtgaag
     6361 aagtgacaac tgcgacagca gatttgacaa ccaatcaagt gaccgttgat gatcaaactg
     6421 ttcaagttgc agatctttct caaccaattg cagaagctcc aaaagaagta gcatcaaatt
     6481 cagaagttgc agagacagtg actgctgcag aagaagtggc actatctaca gactctacta
     6541 cgccagaggg acaaccagcc gaaacaacta gtccagttga agaagcaact cctcaggaaa
     6601 cgactccagc tgagaagcag gaaacacaag caagccctca agctgcatca gcagtggaag
     6661 caactacaac aagttcagaa gcaaaagaag tagcatcatc aaatggagct acagcagcag
     6721 tttctactta tcaaccagaa gagacgaaaa taatttcaac aacttacgag gctccagctg
     6781 cgcccgatta tgctggactt gcagtagcaa aatctgaaaa tgcaggtctt caaccacaaa
     6841 cagctgcctt taaagaagaa attgctaact tgtttggcat tacatccttt agtggttatc
     6901 gtccaggaga cagtggagat cacggaaaag gtttggctat cgattttatg gtaccagaac
     6961 gttcagaatt aggggataag attgcggaat atgctattca aaacatggcc agccgtggca
     7021 ttagttacat catctggaaa caacgtttct atgctccatt cgatagcaaa tatgggccag
     7081 ctaacacttg gaatccaatg ccagaccgtg gtagtgtgac agaaaatcac tatgaccacg
     7141 ttcacgtttc aatgaatgga taaacccgac ttggtaacat cattttgacg aatgagatct
     7201 agctttcgtg ataggaagcg attctcgttc gttttttctt tgtcatactc ttcgaaaatc
     7261 tcttcaaacc acgtcagttt tatctgaaac ctcaaagctg tgctttgagc aacctgcgac
     7321 tagctttcta gtttactctt tgattttcat tgagtatcaa tttgaatgga aaatggaaag
     7381 ttattatctt gtaataatcc aagcaacatt cttgcaatct attttacttt atatcacaat
     7441 tgattggtca actattgata agtcaatgga taggaggaag aaatgataga gattcaagat
     7501 ttactgtatc aactccgctt gtctgagcaa gcgagtacgc aattgtttga aaaaggcttg
     7561 ggattagttt gacacggtat cagattttac tgtttttgct ggagcattct ccttgtaacc
     7621 aaatggcggt tcaggagcgt ttgaaaattg atcaggctgc tttgacacgg catttcaaga
     7681 ttttggaaac ggaaggtttg gtggagcgtc atcgtaatcc tgaaaatcag cgggaagtgt
     7741 tggtagaggc tgcgaagtat gccaaggagc agttagtggt gaatccccct ctgcaacata
     7801 tcaaggttaa ggaagagatg gaaagtatct taacagagtt tgagagaaca gaactcagcc
     7861 gtttattaaa taaattggtt ttgggtattg aaaatataga aatttaagga gaaatagatg
     7921 tcaattattt taacaacgat cgttgctttg gagcattttt acatttttta tttggaaagt
     7981 attgccacgc aatcagatgc gactagtcgt gtatttaata tggaaaagga agaattggct
     8041 catccgtcag taagttcatt gttcaaaaat caaggaattt ataaggctct gctaggagtc
     8101 tttctcttgt atgtcattta tttctcacag aatttagaaa ttgtgactat ttttgtctta
     8161 tttgtgattg gtgctgcgac ttacggctct ttaacagcgg ataaaaaatt attttgaagc
     8221 aaggtggatc agctattttg gccttgatta gtattttact ctttaaatac acttgaaggt
     8281 tgattctaat ctcgctaatc ctttttaatc cagaataagg gaaatatgtt atacttgttt
     8341 ttaagaaaaa agtctcattg aattggtttt gaggagttag aaatgaaagt attagtgaca
     8401 ggttttgagc ccttttgagg ccattaaagg tttaccagct gaaatccatg gtgctgaggt
     8461 ccgttagcta gaggtgccga cagtttttca caaatctgct caagtattgg aagaagagat
     8521 gaatcgttat caacctgact ttgtcctttg tattgggcaa gctggtggaa gaactagttt
     8581 gacacctgaa cgagtggcca ttaatcaaga cgatgcacgc atttctgata acgaagataa
     8641 tcaaccgatt gaccgtccca ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc
     8701 gattaaagcg atggttcaag atataaaaaa agagggctta ccggcctctg tttccaatac
     8761 ggcagggact tttgtctgca gccatttgat gtatcaggct ctctatttgg tagaaaagaa
     8821 atttccatat gttaaggcag gttttatgca tattccttat atgatggaac aggtggtgaa
     8881 cagaccgact actccaacta tgagtttagt ggatattcgg cgagggatag aagcagcaat
     8941 cggcgctatg atagaacatg gagatcagga actcaagttg gtaggcggag aaactcattg
     9001 atagaaaaaa gcttgagggg aaaaaccttc aagcttttgg acgttttcga gccaatactg
     9061 ctcggtaaaa cataatttta gtgcattgga tataaggtag gagtgaaaaa ctagcaatgc
     9121 caaaggtaat ccaattgagg aagtaccaag gaagaagctg taaatctagg acaaagtgct
     9181 ggaacttgta gcccttcata aaggaacggc tagtttttag gattcgtctt ggtgggacct
     9241 gtcctaggtc tagactataa cagagaagaa attccacctg tgaataggca taatactgtg
     9301 gaatatagag gatatttcct acaatgatca agatgagact tgcaagaaag tagagtccaa
     9361 agaccatgag gaaacgctcg gtttcaactg atgagagatc tagatttgga aactcaggat
     9421 gtagggtgac gaattttttg gctaaaaagc tactataaaa gaggaggtaa atcccaagta
     9481 aattagggat actccataaa aagagataga aacgtttgag aagtagggtc aaaaaggttt
     9541 gagaaaagcg ctcctcatca aagagagcta ggctgttttt tacagatggc tccgttttag
     9601 aatctttcat gagtgtcagt gttgcataga cggaactggt caaaagaata gtcccgataa
     9661 aggagactag tagaggaaag aggtaggctt gaagtatttg gccaagtatg ctgaaaaatg
     9721 gctgttctaa aacagtcccg tggatccgag ataagggatt aagaaaacta gataagatga
     9781 ccagcatact gggaaggata tagaggagaa agagacgggg ggtgtcagcc tgaaaatgtt
     9841 ttgactcctg acgaattgtt tttaaatcaa tttttggata gttcattctc ttattatacc
     9901 atagttctta tacatagttc gtgacagttc ctactttttt tgataaaatc atacagtgtg
     9961 tccttgggca cactgtatga actgggactg tctttcccag cttcggaggt aaaaaatgtc
    10021 agattcacca atcaaatatc gtttgattaa gaaagaaaaa cacacaggag ctcgtctggg
    10081 agaaatcatc actccccacg gtacctttcc gacacctatg tttatgccag ttgggacaca
    10141 agccactgtc aaaactcagt cacctgaaga attgaaggag atgggttcgg gaattatcct
    10201 atcaaacacc tatcatctct ggcttcgccc tggagatgaa ctcattgcac gcgctggtgg
    10261 tctccacaag ttcatgaatt gggaccagcc tatcttgaca gatagtggtg gttttcaggt
    10321 ttattcttta gcagatagcc gtaatatcac agaagaagga gtaaccttta aaaatcatct
    10381 aaatggttct aagatgttcc tatccccaga aaaagccatc tctattcaga ataatctggg
    10441 ttcagatatc atgatgtcct ttgatgaatg tcctcagttt tatcaacctt atgactacgt
    10501 taagaaatcg atcgagcgta ccagccgttg ggctgagcgt ggtttgaagg ctcaccgtcg
    10561 tccacatgac caaggtttgt ttggaattgt gcaaggtgca ggatttgaag accttcgccg
    10621 ccaatcagct catgatcttg tcagcatgga tttctcaggc tactctatcg gtggtttggc
    10681 agtgggagaa acccatgaag agatgaatgc ggtcttggac tttacaactc aactgctacc
    10741 tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca gatagcttga tcgatggggt
    10801 cattcgtggg gtggatatgt ttgactgtgt cttaccgact cgaattgctc gtaacgggac
    10861 ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc cagtttgctg aggactttac
    10921 gccactggat cctgagtgtg attgctacac atgtaataac tatacacgcg cttaccttcg
    10981 tcacctgctc aaggctgatg aaacctttgg tatccgcttg actagctacc acaatcttta
    11041 cttcttgctt aacttgatga agcaagtgcg acaagccatc atggatgaca atctcttgga
    11101 attccgtgag tattttgtgg aaaaatatgg ctataataag tcaggacgta atttttaaaa
    11161 tggaattgat ataagctaaa aatcctaagt tttctcttag gatttttctt ctttttttga
    11221 tagaataaag tgtacaatga aaggaagaat aaactcgtat gcgcattaaa tggttttcct
    11281 tgattaggat tataggttta cttttggtac tcttgtatca cttctttcag acgatctttc
    11341 ctggaggatt tttcggggta gatgtctttt tcacattttc aggtttcctg attacagctc
    11401 tactcattga agaattttct aagaacaatg agattgattt gataggattt tttagaagac
    11461 gcttttatcg gattgtgcca cctgtggttt tgatggtctt ggtgaccatg ccttttactt
    11521 tcttggttcg ccaagactat gttgctggaa ttggtggcca gattgcgagc gtcttaggct
    11581 ttatgaccaa cttctatgaa ctcctaacag gtgggagtta tgaatctcag ttcattcctc
    11641 atttgtttgt tcataattgg agtttggcag ttgaggttca ctactatatt ctttggggat
    11701 tggcagtttg gttcttatcc aaacaagcta aatcaaatgg tcagttgaag gggatggtct
    11761 ttctcttatc tgctgttgcc ttcttgatca gtttcttctc catgtttatt ggtagttttc
    11821 tagtgacctc ttattcctct gtttatttct ccagtttaac tcatgtctat ccattctttt
    11881 taggaagtat gttagcaact attgtaggcg ttcgtcagac gacttccctc gtcaagcagt
    11941 tggataaaat ctgggattta cgaaagactt tggtagtttt tggaggaggc tttggtttcc
    12001 tagttctttt gactttcttt gtcaaattca cttatctttt tgcctatctt atcggcttct
    12061 tacttgccag tcttgcagct cttgccatga ttctggcggc gcgtgtctta catgaaaaga
    12121 cacatcatat acaggagccg aagattatca gctttttagc ggatactagc tatgcggttt
    12181 atcttttcca ttggcctttc tatatcattt tctcacagtt gacatcaaat cttcttgctg
    12241 tattactgac tctgatttgt tcttatggct ttgccagtct gtcattttat gtattggaac
    12301 cttggattgc aggcaagaac acacctattt tacaaactct tcgtcccctg ccttatattc
    12361 acgcaattct tgcagcaggt acaggaatct tgaccttcat tgtctgcatg gtgaccttgt
    12421 tggcaccaca agtgggagcg tttgagacag acttgactgt caatggcttg aagcaagctg
    12481 caacaaatat tggccagacc aaggtgatgg cagaacgggc agatgcaaac agtttgggaa
    12541 ttgctgatgg cactatgtta attggtgact cagtggcttt aagggcaaat acagcactac
    12601 agacagctct tcctggagca cagattaacg cgcaggtcag cgtaacaacc aagaccgcaa
    12661 atgaaatcat gctaaataat agccagaata aatttttacc taagacggtg gtcattgcga
    12721 ctggggtaaa taatcctgag aattacaagg atgactggga cagtatcgtg aaaaatcttc
    12781 ctaagggaca ccatatgatt ttggtgactc cttatgaggg agataagaca aaagagacct
    12841 atgccatcgt tgagaaggct gctgcctata tgagagaatt ggcagagaag acaccttaca
    12901 ttacgatagc agattggaat caagttgcga aagagcatcc agaaatttgg gcaggaacag
    12961 accaggttca tttcgggagt gagagtagca ctatcgaagc aggagcaaaa ttgtatgcag
    13021 atacgattgc cacagctttg cagacagctc aagataagcc ggttaaatca aaataacttg
    13081 tattaaaaaa gagaagagtt ggagaaatcc agctctttta aaatatctcc aaaaaatagg
    13141 tctataccat ttacaaataa aaaagaaagg tttataatgt aattgacata ataaattgta
    13201 gaatcaatct tttaaggagg ttaacattat gcctaactat attaaagcgg atcagttttt
    13261 ctacccacac ggagttcgtc gaggtggtta cttggaactt gtggacggca agtttgggaa
    13321 acatgtagag cagattcctg aaggggctga ggtgattgac tatacaggtt atagcattgc
    13381 cccaggtctt gtggatactc atattcatgg atatgcaggt gtagatgtga tggacaacaa
    13441 cattgaaggt acattgcata ctatgagtga aggacttctt agtaccggtg ttaccagttt
    13501 cttacccaca actttaacag ccacttatga gcaattgctt gcagtcactg aaaatcttgg
    13561 aaaccattat aaagaagcaa caggtgctaa gattcgtggg atttattatg aaggtccata
    13621 tttcacagaa acttttaagg gggcacaaaa tccaacttat atgagagacc cgggtgttga
    13681 ggagtttcat tcttggcaaa aagcggcaaa tggcttgctt aataaaattg cccttgcacc
    13741 agaacgtgat ggggtggaag actttgttcg tacagttacg ggcgaaggtg tgacggttgc
    13801 tcttggacat tcaaacgcga cttttgatga agccaaaaaa gcagtcgatg ctggagcgag
    13861 tgtttgggtg catgcctaca atggaatgcg tgggttgact caccgtgaat tgggtatggt
    13921 tggagccatg taccaattgc catataccta tgcagagttg atctgtgatg gtcaccacgt
    13981 agatccaaag gcttgcgaaa ttcttatcaa acaaaaagga acagaaaaca tcgctcttat
    14041 cacagactgt atgacagctg ggggattgga agacggagat tatatgttgg gagaattccc
    14101 agttgtcgtt gcaaatggaa ctgcacgcct caaatcgaca ggtaacttgg caggttctat
    14161 cctcaaactc aaagatggtt tgaagaatgt ggtcgaatgg ggaattgcga atccgcatga
    14221 agcagtcatg atggccagct tcaacccagc taaatccgtt cacatcgatg acgtctgtgg
    14281 ccaaatccgt gaaggctacg acgctgactt catcgtatta gataaagatt tggaattggt
    14341 agcaacctac ctagatggtg tgaaacgtta tcaagcataa gagagaaggc agaagttaga
    14401 gactagcttc tgctttttta tacagggtac tttactggta aataaaaagt taaaaaaatc
    14461 acaaaaaaag cttgaagaaa caaagaaaaa ggcttacaat tgaactataa atattacaaa
    14521 taaaaaaacg gaggagtgct ttatgaaagc ctatacttat gttaaaccag gacttgcttc
    14581 ttttgttgat gtagacaaac cagttattcg caagccaaca gatgctattg tgcgtattgt
    14641 aaaaaccact atttgtggaa cagacctcca tattatcaaa ggggatgttc ctacttgcca
    14701 aagtggtacc attcttggcc acgaagggat tgggattgtt gaagaagttg gggaaggagt
    14761 ttccaacttc aaaaaaggtg acaaggtctt gatttcttgc gtctgtgcct gtggtaaatg
    14821 ctactactgt aaaaaaggaa tttatgccca ctgtgaagac gaagggggct ggattttcgg
    14881 tcacttgatt gatggtatgc aggctgaata tctacgtgtc cctcatgcag ataatactct
    14941 ttaccatact ccagaagact tgtcagatga agctttggtt atgctgtcag acattctgcc
    15001 tactggatat gaaattggtg tcttaaaagg gaaagtagaa cctggttgca gcgtagccat
    15061 tattggttca ggtccagttg gattggctgc tcttttaaca gcccaattct attcaccagc
    15121 taaattgatt atggtagacc tagacgataa ccgcttggaa actgccctat cattcggtgc
    15181 gactcataag gttaattctt cagaccctga aaaagccatt aaagaaattt atgatttgac
    15241 agatggtcgt ggtgtggatg tcgctatcga agctgttggt attcctgcaa catttgattt
    15301 ctgtcaaaag attatcggtg tagacggaac ggttgccaac tgtggtgtgc atggtaaacc
    15361 agttgaattc gatttagata aactttggat tcgcaacatc aatgtaacaa ctggtttggt
    15421 atctacaaat acgactccac aattgttgaa agcacttgaa agtcataaga ttgaaccaga
    15481 aaaattggta actcactatt tcaaactcag tgaaattgaa aaagcctatg aagtcttcag
    15541 taaggcagca gaccaccatg ccattaaggt cattatcgaa aacgatatct cagaagccta
    15601 agtagtaaaa atatttttgt acataagtaa atagaaattc agtcatccat cagatggctg
    15661 gattttttat caaaaaatta agaaatgagc atatttcttt ccttgtctgg cggaattggt
    15721 tataatatac ggtacaaagg aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga
    15781 ttttgaaccg tggtggttct tagaaggttg ggaagaagat attgtagcaa gtagaaaatt
    15841 tgaccagtat tatgatgctc tcaaatacta caaaacttgc tggtttagat tggaacaaga
    15901 atcccctctt tataaaagta gaagcgactt gatgaccatt ttttgggacc cggaagacca
    15961 acgctggtgt gatgaatgtg atgagtattt acaacaatac cattctttgg ctcttttgca
    16021 ggatgagcag gttatcccag acgaaaaact acgctcaggc tatgaaaaac aaaccagtca
    16081 ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga aaagtaactt ttttggagtt
    16141 gcttttttta tttttctaac gctttgcgaa tagtataggt gaggaggtaa gtatggttca
    16201 agaaattgca caagaaatca ttcgttcagc tcggaaaaaa gggacgcagg atatttattt
    16261 tgtccctaag ttagacgcct atgagcttca tatgagggta ggagacgagc gctgtaaaat
    16321 tggtagctat gattttgaaa agtttgcagc cgttatcagt cactttaagt ttgtggcggg
    16381 tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc tgtgattatg cctatgacca
    16441 gaagatggcg tctctacgtt tatctactgt aggcgattat cggggacatg agagtttggt
    16501 tatccgtttg ttgcacgatg aggagcagga cttgcatttt tggtttcagg atattgaaga
    16561 attaggcaag cagtacaggc aacggggact ctatcttttt gctggcccgg ttgggagtgg
    16621 taagacgacc ttgatgcatg aattgtccaa gtcactcttt aaaggacagc aagttatgtc
    16681 catcgaagat cctgtcgaaa tcaagcagga cgacatgctt cagttgcagt tgaacgaagc
    16741 aatcggccta acctatgaaa atctaatcaa actttccttg cgtcatcgac cagacctcct
    16801 gattatcgga gaaattcgag acagcgagac ggcgcgtgca gtagttagag ctagtctgac
    16861 aggtgcgaca gtcttttcaa ccattcatgc caagagtatt cgaggtgttt atgagcgcct
    16921 gctggagttg ggtgtgagtg aggaggaatt ggcagtcgtt ctgcaaggag tctgctatca
    16981 gagattaatc gggggaggag gaatcgttga ctttgcaaac aaagactatc aagaacacca
    17041 gccaactagc tggaatgaac agattgacca gcttcttaaa gatggacata tcacaagtct
    17101 tcaggctgaa acggaaaaaa ttagctacag ctaagcaaaa aaatatcatc accttgttta
    17161 acaatctctt ttccagcggt tttcatctgg tggagaccat ctccttttta gataggagtg
    17221 ctttgttgga caagcagtgt gtgacccaga tgcgcacggg cttgtctcag gggaaatcat
    17281 tctcagaaat aatggctagt ttgggctttt caagtgccat tgtcactcag ttatccctag
    17341 cggaagtcca tggaaatctc cacctgagtt tgagaaagat agaagagtat ctagacaatc
    17401 tggccaaggt caagaaaaaa ctaattgaag tagcgaccta tcccttgatt ttgctgggtt
    17461 ttcttctctt aatcatgctg ggactacgga actatctact accacaactg gatagtagta
    17521 atattgccac tcagattatc ggtaatctgc cacaaatctt tctaggaatg gtaggatttg
    17581 tttcagtagg tgtcctttta gcactcactt tttataaaag aagttctaag atgcgcgtct
    17641 tttctatcct agcacgcatt ccttttcttg gaatctttgt gcagacttat ctgacagctt
    17701 attatgcgcg tgaatggggt aatatgattt cgcaggggat ggaactgacg cagatttttc
    17761 agatcatgca ggaacaaggt tcccagctct ttaaagaaat cggtcaagat ttggctcagt
    17821 ccctacaaaa tggccgtgaa ttttctcaga cgataggaac ctatcctttc tttaaaaagg
    17881 agttgagtct catcatcgag tatggggaag tcaagtccaa gctgggtagt gagttggaaa
    17941 tctatgctga aaaaacttgg gaagcctttt ttacccgagt caaccgcacc atgaatctgg
    18001 tgcagccact ggtctttatc tttgtggctc tgattattgt tttactttat gcggcaatgc
    18061 tcatgcccat gtatcaaaat atggaggtga atttttaaca tgaaaaaaat gatgacatta
    18121 ttgaaaaaag cgaaggttaa agcttttaca cttgtggaaa tgttggttac taaaattatt
    18181 tatattttcc acttgataac aatgtcctca gctgttacct gaaccttgtt tataagccct
    18241 ctaacaagta ccttttgacc ttcgtagtcc attgaaaaga ctttttcagc gtttagcagt
    18301 ttcctcatat cagcctttct tttgttcttc ctgagcgctg gatcgttttc cagttcagtc
    18361 tcaagagtag ccctcatgct tataaattcg gctgacttgc tctgtaattc ctccagcgtg
    18421 atacggtcgt ctatgtagag gtcgttaagt ctgctcagtt tctttgatag ctcctctatt
    18481 tgtttcttat agctctcacg gtctatagtc tcatcattgt ctcctgaaaa tattttgtcc
    18541 aggtaatcag cgttatcttg tagcttgctt atttctttta gcacagaggc ctctagcttg
    18601 tctttgtagt aaaatcctga gtcacacttt ttattgtcgt tgtaggtagt aacgcctctc
    18661 agcgttcgtg ggtgcctttg atggcattca tattttttta acctgctccc atctttcctc
    18721 tttacgccta acataatttt taaaggagcg ccacaatatc cacattgggc gatgccggat
    18781 aaaatgtact tagcttggaa tggtctagga ttgacattct ctgctgctgt tctttgtctt
    18841 atttttagct cagattgagt cttatcgtat tcctcttttg aaataatcgg ctcatgatta
    18901 cctggataaa tttctccctt atactgattg aaaccacaat agacagggtt atcgagtatg
    18961 gttctgaccg cccgatagct ccaaggcaca tgctttgggt atttctcatt tagatcatct
    19021 ctcaacttag taatagatct ccctctcagg taactctcaa agataaactt aatggccaga
    19081 gcctgagctg gattgatagt gatagttcct gtatctctgt ggtagtcgta tccataggat
    19141 gttttagccc acatcatgga tttaccagat ttggcacgtc ctagcttgcc aagttgcatg
    19201 cgttccttga tttgctccct ttctagctga gcaaagacgc tcaagagtcc aatcatagcc
    19261 ttaccaaaag gagtagaggt gtcaaagttc tcttgcaagc tcagaaattc aatcccattc
    19321 ttgatgaata catcctcaat caagtgaagc gtgtcttttt gactacggct aagacggtcc
    19381 agcttataga ctagaactgt gtcaaatttt ctttttttag cgtctttgat aagactttct
    19441 agcgctggtc tgtcagtatt ggatcctgag aaacctccat cagtatatac tttgtataca
    19501 ttccagtctt taatgtcgca gtagctagag agcttgtctt tttgctcatc tatagagtat
    19561 ccctcctcaa cctggtttgt cgtcgatacc ctgacatata tagctacttt atttgttgtt
    19621 atcatagtag tacctctttc aaaatttcct aaaaaatgat aaaatgggta caagaaaaga
    19681 catcatgaga ggttgtctcc atgaaaatct tttcctgtca catgcctcac gctcagagtc
    19741 gccaaacttt gagagcgtgg ggcttttttg agttgtttcc aaaatggaaa cagttgctag
    19801 ataaaaagaa aagtagccgt atcaaatacg gctaccatca cgttatggat ctaaaatcca
    19861 aacctaaact ttatggagct aaactcctga tagctgtatt gtaatataat tatttaaaaa
    19921 cgtcaatttt ttatttaata taaatctagt ccaagaactt cttttatctt gtctagtagt
    19981 tttttatggt cgtttgtatc tagcttgtaa gctggtctat aaactttatc atataactct
    20041 tttcctgctg catctagcat tacttgtcca tcactatcag tctttttctt tgttttatag
    20101 acgattttcc gtttgtctat ttcttggatt tttcggacat aagcataggc tttgggcttg
    20161 gacggagtgt tttgatattt actattttca atcgtgatac caccacgata tttctttcct
    20221 gattttttac cagttaaagg tgctactagg agcgttccat caactttgtc aggagttgtt
    20281 aagatgatag cgtagtgttt tccgtaaaat tcattcccac ctttttgtgg gaagttgatt
    20341 agataaactt cgccttgttg aaatttcata ttattgctcc ggataataaa ataaggtgta
    20401 cctcttagaa gtacaccaga ccgttttgtc cttgacgaac aaggctttat aaataaatat
    20461 cgtatccttg acgaacaagg cttttgacat tattagtgta cttctttttt tagtttttgt
    20521 caagtagttg tttccaaaat ggaaacagtt ggttttattc tttcgataag tgttgttgaa
    20581 gaattaaggc cacgttggct ttctcttcct ctgtcatagg aggttcgttt ggatcgtcta
    20641 ccgaaaactc gatagcatgc catttatcat ttactctaat ccactctctt cgtctgtggc
    20701 atttgcaatc taggttgtgt ttaatcactt ccatcggtct actttcgtta ctcatgttat
    20761 ttctccctat acacactgac aacttcccca atagttcgga tgtcgttgct ttcgtctagg
    20821 tgtatatcct cataatctgg attcaagctt tccagatatc cctgacgcag tttcttaaca
    20881 tagttagcgc cgtctacttg gaagatgccg atagtgttat aatcaacctg ttgggtattc
    20941 tttataaaaa gatagtcacc attctttatc tttggctcca tagagttgcc gacgacataa
    21001 gcgatagcgt cgtagtcgtc tgggatttca tcctcataga acgaaacctc catatctaaa
    21061 tcgtcgtcct gtatcgaacc actaccagca gagacaaccc cagtaacacg tcggtaagta
    21121 gtctgtctgt agtcgtccag tctgatgatg ttctccgata cttcgtttat cttcgctttt
    21181 tttcgtttct cttgttcgtc acgtaatttt tcagcgtatg ttacaacttt tctttgtcca
    21241 ttcggttcta gttcgtcata tatggattgg atttgagatt ttgaaagaac agctggagta
    21301 gcgttgattt gaatttcttg ttcatcatct agcatattaa ttaaatcatc tgtagaaatt
    21361 tgcatccctt tagcgatttt ttctattgtg tcataagatg ggacaatggg tttttttgat
    21421 ttcggatgtt catttttttc aagcatagaa atatatcctt ttgttaaatc agataattca
    21481 caaaaagcat ccatcgataa tttatgctct gttctatatg atttgagtaa ttctcctaac
    21541 ttcattcaaa aacccctttc tatatattgt ttaatctatt atacatcttt aaataaaaaa
    21601 agtaaacatt ttttgtttaa cacgcttgac attttatgtt taacgtgtta aactataatc
    21661 aagcttaagg aaataacaaa aacaaagcgg agggaaacac catgaacaca ttaaacgaga
    21721 aagcaatcaa catctttaaa gcagtagcaa aggaaacttt aatccaaggc acttacgagg
    21781 aaaatttcct ctatagcaaa cttgaagcat tctgtactaa ctgccgtcaa ttcgctttcg
    21841 gatggacaga gttagcagat gagattgaac gccaagagcg ttaccttctc gattctggtt
    21901 tcactcaaga ggaaatcgat gacattcgtt ttgatgcagc atttgcagga atgcttgata
    21961 aattaaatgt agcctgattg gtatcaccaa ggttcgaatc cttggcaggt tgttgctcat
    22021 agagcgaaaa aagagagaaa ggagtagaac gatgaatgaa tcttttctta cgatactagg
    22081 catatcaatg attgctagtt ttatcacgaa tttaattgct tacttagctg gtaagcatca
    22141 tctgaaaaag aaaattaaaa accacaaaat gtggtttgat tctgagatag agcgtatcaa
    22201 gaaaaagtat catttgtgat ttttcttgga taattttttc acaaattgtt tttgaagatc
    22261 ggaaggttgt tgcttgttcg cgaaatgact aagagtgctc atatttttta ttgctacatc
    22321 tgcagatatt tctcctgaaa tagctttaaa aattaggtca tttatcttta agccttggtc
    22381 ggtttgagtg tctagttgag atactttttc aagttcaagt aatcttaatt cgtgagtttg
    22441 ttgcagcgaa taaagttctt gtgagtgttg cttttgcatt ttttccatat cttgtttaaa
    22501 ttgattttcg acattttcaa gctcttgtga gtgtgcaatg ttaattttat caatttcatg
    22561 cttgctgttt cctttagcag cgatgtaaga ccacatgcca gaaacaaagg caggtatggc
    22621 agcgatcgca agtgtttcta taaaactaaa attattcata agatttctcc aatcatttta
    22681 ttttgattat accacatttg aaaggtagtt agaattggaa gataaaatca ttgaacttgc
    22741 tgattacttc atcagcgaaa acacaacgta cagagaagct aaaatagcgt gtgagaagct
    22801 atttcttcaa gtcagtcatg agatagaact cagggcaatg gaaagtagga cgagggtatg
    22861 aaagaaaaac taaacgaatt tctaaaattc agaagccagt ttacaaaacg agaatggatt
    22921 gaaattaacc aagttgtcga agctcgttta aatgaaaaag ccgaccagtt gaaactggac
    22981 gactcagatg tagaaatcat ttctaaaaga ctaggacggt ctatctaaag aaagggtcaa
    23041 acaaatggcg tctaacagaa ctatatcagt aaatacatca gagcatgatg tattgttgac
    23101 ggcaagaaaa aaccaccctg ctgtattcgt cgatggaatg tttctcgacg gagttgagcg
    23161 agtggaattt accaatcatt atctggagaa gtgtgaagtt gttttaacgt ttaacgatag
    23221 agttgaaacc aatcccttcc ctctaaacga tattacttta ttagaaaagt tatttggtca
    23281 ggcttcaaac gggcaatcct tacgggatat tgtcgtgcaa attcttgaag atgctgatta
    23341 gcatctaaac catcaaaaaa agaaacatga acactaaaac tttcttttcc gtttttcttg
    23401 gttctatcaa attctttgcc aaggacgatt aaagaagttt ctagctggtg atcagtcata
    23461 acattccctc ctttctgact acattatagc agaattgcga ggaacaaata gaaaaataag
    23521 gaggtaggaa tgtgcaagga gaacgtttaa gaaaatggcg tgaaaaagag aaaatgtctc
    23581 aagaggaact tgcagagaag tcaaatgttt ctcgaacaac aatacacttg attgaatcag
    23641 gtcagtcgtc aacagtgaaa attcgaacac ttcaaaaatt agcagtagtt tttaataagc
    23701 aagtgaaaga ttttttttaa agaaaatgtt taacaaatta aacaataaag aaaggagaat
    23761 gtatgacaga ctttaaagat ttggattgcc aatttatctt tcaggaatcc aactaacgac
    23821 tataccgctg ttagtaatag ttttatcaac gatcctgcga tggattttac agcggttggc
    23881 atcatgatgg tggtgctggc caatcaccca aactggcaag tctatccgga tgagatagcc
    23941 aaaagaaaag gtgttaaccg aaagacaatc gataagtatt tcaaaatctt tgaagaggct
    24001 ggatatttgc gaaaaatcag aaaaaaacct cctggaaatg gagggagtca tatattcaga
    24061 ttcttttcag atgtaaaaat atctgatttc caattcgata ttatgaaaca gagattaaac
    24121 ctgtctatca aaaaggcgtc tatgaattat aattctgaca ttccaaaaag tgagatgtca
    24181 gaaagtgaga tgtcagaaag tgagatgtca gattttgggc actaataaat attaactaac
    24241 aacaagtatt aaataacaat aaatattaaa aaacaacaag tcctacttct ttaaataaaa
    24301 gagagaaatt tcaattttag gactttgaaa aataggaaag gagaaaaaat gagaccaaga
    24361 cgatatccgt atagtgggaa aaaagagtcc acctttgtaa aggcagaccc tgaattagta
    24421 gaaaaaattc taagaaacac tagttttctt gagagtttac aaaaaaagcc tatcaatttt
    24481 cagatagact cagaagaatt taagcgtctt agctatgaag ccattcatga tacttctcaa
    24541 gtaactcaat agtagttatt acagaagtca aaccactgac cttccccagt tgcaatccgt
    24601 ctgtatggtc aatctgttta gtagcttcat tagctttagc agagatagct tgcatatctt
    24661 cagctgttaa agattctcga aaatctttaa aggatttcat aaaaactcct cctttctatt
    24721 ggaattttga ctaaaacggt gagaggtcct agtcgagatt attatagcaa tttaggagga
    24781 tattacatcg gtcttgaggc tgatttttgg aggcaatatt ggaagataaa atcatagaac
    24841 ttgctgacta cttcatcagc gaatctacaa cgtacagaga agctaaaata gcgtgtgaga
    24901 agctattgaa acaagtcagc catgagatag aactcagggc gatggaaagc aagacagtct
    24961 agaagacaac aaaaagcacc tgacggcaat caggcgctca acaaaagtat tcgaggtaat
    25021 tataacatga agagaaaaaa agagcaatgg aaaccaagaa ttgtaaacat catggcagat
    25081 ggctctcaag ttgatgattt gacaggctat gtcatccctg ctggtcattc ctactatgac
    25141 attattttag gcatgaacaa gcaatctaac gaggagggcg tagcttaatg aaattactta
    25201 ccaagttaaa actcagactt gaagtagttc ttaaagcagt caaccttgac tggcgagagg
    25261 tagcggtcga actcatgatc gacctatttg aggagcgcaa gcgtcgcttt gctttcgagc
    25321 aagaaaacta tgacttgaag caacaattgg caatctacaa agaaaaagaa caaggagaac
    25381 aatatgttta aagcactaaa aacaatcaaa aaaatcaaac agcttcagaa agaaatgcac
    25441 gatgtcagtt tagcctttct ggctctacaa gatgtcggat tgatgccaga gactgaaaga
    25501 agcaaggcga aggctcaaac aatgcacgat gtaagccaca tgctcaagga cgtcctgggc
    25561 ggcaagtcgg tagatgaagc catgaaacgt ctaaatagcg aagtgaaaat tgaagatgtg
    25621 gagcaggaag atgactagaa ttgaacttga aaaccgtgta tggcttttgg ctaatcatga
    25681 agaaaaaaaa cgaattgctg gatcttgggc taacgtcaaa gaccagatat gtgaagcgag
    25741 ttctggaact aggaaaggtg tatgctcatg tttgattacg acagagacat aatgcagcct
    25801 cctgaaccca gggaagaacg tgacccagcc gattggattt tcagcgctgg tcaatggatc
    25861 tatgtaggag attgctagcc taaaggagaa aaaaatgacg cagatgacca ataaaggaaa
    25921 gtcgttcata agagcagaga tctctgaaaa acaaaaagaa tatatcagac ttcttgctga
    25981 attgagaggc gtgacaacac aagaacttct aggtcaagtt gtagaacgct tcattgaccg
    26041 gaatttgcaa cttattcaag attacaaaaa tgaattagat gacttaaata gtaagtctag
    26101 acgcagaatt gacatgaaca cataggagaa aacaagatga ctaataatca attagcaaca
    26161 cagacaaaac gtaacatcac tactgaccca agtttattga ccggggcaga catcaaaaag
    26221 tattttgacc cacaaaacct actgactgaa aaacaagtag gtcaggcgct agccttgtgt
    26281 aaaggtcgca atcttaaccc atttgctaac gaggtctaca ttgtagctta cacaaaccgt
    26341 aatgggggca aagagtttag cttaattgtc tctaaagagg ctttcttgaa acgtgccgcc
    26401 caatgtaaag actatgaggg ctttgaggct ggagtagtgg tagtagacag tgagggtgtt
    26461 atgcacgaac gcaaaggggc aatcatgctc ccagaagata cactgatagg cggatgggct
    26521 agagtgcacc gcaaaaattt caaggtacct gtggaaattt ttgtcagtcg tgaggaatat
    26581 gacaagaaac aaagtacatg gaacaccatg ccagctacca tgatcaggaa agtggctcta
    26641 gtaaatgctc ttagagaggc tttccctgag gacttgggga acatgtacac agaggacgac
    26701 ggtggagaga catttgaccg tatcaaagac gtcacacctc aagagagccg tgaggatgtc
    26761 gtagcacgca agatggctca gattgagcaa ttcaacaaag agcaagaggc aaatcatgca
    26821 gatcctgaac ctgctaaaac tgaggagcca atccagggcg agttgctaga cggtgaactt
    26881 gaatattagg aggacaacat gcaagaatta caggttattg atgataagaa aatcaataaa
    26941 atctatgaga tgatcacaac ggatgaactt actagagagt cttttgaaaa agacctcata
    27001 gaggctactg aaaagtacaa ggactatatt cctacagcta gcactctcaa agacgacaag
    27061 gcaaagcggg ctgaatttaa caagttaatt gagtctaaaa atcgtatccg tattgacact
    27121 aaaaacttac tatcagagac agctaacaca tgggatagtt atgctaagtc aattattgac
    27181 ccatttgcaa ccgtagttag tgaatttgac aaaggtatca aggaaatcga agaacatcaa
    27241 agacaactaa aaatagatac ggttaagagt tacctagcca acaaatcggc tgagtacatg
    27301 ctggatcctc gtctctttga tgaaaaggcc cttgagtatg tcaaggctgg cgatttcatg
    27361 gcagatggcg tgacgcttaa aaaagccact atgaagtcac ttgatgacat ggtcacattt
    27421 gagtttcaga aacagcaaga atttgaaaag gctaagtcag ctatttcagg gttatgtgct
    27481 gagtatggca tgactgactc accttacatt agacagctga aagacttgac tcttgctgaa
    27541 gtctttgaac aaatcaaagc tgattatgag tctgaaaaac aaaaggaaga aatcagacag
    27601 gctcaagaac gagcagagcg agaaagtcag gaacttttag cagcccaaca aaccaaacag
    27661 caagaacggg ctccaaaatc aacgggaacc ccaaattttg acccagaggc aggcgaaatc
    27721 ttggacggtg agcaaatccc tcaaaatgag cagaactctc ttagaggggc tgaaaatgac
    27781 ctaaaacgat atacccaaaa aatgacttta gaggtgtatt ttgtagacac agccgaaaaa
    27841 gaccgtttca aggctggtct aagtcaactc ggatttgatt ttaaaaagaa ctatcaagtc
    27901 agcggttatc aacgtattga gccattgact caggctgaac tcaatgagca atggggaggt
    27961 aaccatggac atcagaaaaa tatctgacag cgtagccatc tactcggacg gcaagaaatt
    28021 gcatgttatc cacaacctag gggatgagtt tatcctagat tttaaggtgg gagaggatag
    28081 cgtctggaac ctcaatggcc aagtcgtaga aattattgac atgattgagc ctgtctttaa
    28141 agttttcagc ttttgctcaa aatctggaga gggtatgcaa cgcttaaaac atgctatcgt
    28201 ccactttgaa atatttgagc agtacatcag agataatcag gaagacctga tgatctggtg
    28261 gcacaatcca ggaggggaat atgattaaaa ccgtattttt ttcatgtgat tatccacatc
    28321 atgaggcgat tgacgaccaa ataaatagct ggcttgccga aaatccaggc attaagttga
    28381 ttgacatcaa atttcaatca aatgtgtctg ctgtcgctga cagtggagtc agtgctgaat
    28441 attggcatac atccgcattg attatttaca aagttccctc agagaacaat ataagcagta
    28501 ttaattcaaa tggtttaggt ttcataatca gctgtgagaa atgtggtagc ttatcaataa
    28561 tcaagggaaa agatgtaggt caaaatgtat gttatgaatg caaaggagag aaataatgaa
    28621 tgattttatc aaagagattg ggatggctat cctatggatg tttttagggt atctcttggg
    28681 agagcgtagc actagagggg gacaaatcag atgatcaata acgtcacact ggttgggagg
    28741 cttgtagcgc ctcctgatct acgaaaaacg cctaataatg tatctagttt acagggcacg
    28801 cttgcagtca atcgcaattt caaaaacgaa aatggagagc gtgaggctga ttttatcaat
    28861 tttcaagctt ggagaggtac agctgacatc attgctcagt attgcagcaa gggctcactt
    28921 attggcctca cagggcgctt acaagttagg tcttacgaga aagacggtca gcgtcgatat
    28981 gtgactgaag taatcgctga gagtgtagct ctgctagaga gtcgcaacag tcagcacgga
    29041 caaggcaaca gtttccaaaa tgggaatagc tcacctttta ccgatcctaa cccctttgac
    29101 ctcccaaatg acggtttgcc gttttaggag gtatatatgt caaaaattaa aattcttgac
    29161 gcttgctgtg gcagtcgtat gttttggttt gataaaaacg aaagtcacac aatttttatg
    29221 gacattaggc aagaaacatt tgagatacat gacaaaaagg tcaatgtaga ccctgatatt
    29281 atcggtgatt ttcgtgacat gccttttgaa gacaacacat ttaatctagt tgtgtttgat
    29341 ccaccacatc taaaatgggc tggacctaat tcgataatga aagctcagta tggacagctg
    29401 gacaaagtta cctggtcgga agatttggcc aagggttttg aagaatgtat gagagttcta
    29461 aaagttggcg gcacactagt ctttaaatgg tctgactgtc agataaatgt aaagaaatta
    29521 ctagaggtga taccattcaa gcccttattt ggtcagcaaa gaggcaccac acactggcta
    29581 acgtttgtaa agtttgagga ggacaagaat ggagtggaca gattgggtgg attggaaacc
    29641 tgaaaccaaa acggacatta aaaccaaaat tgaaaatgac gggtacactt ttccacacta
    29701 tgacaagaaa aacaacggcg tcaagtacgt catctctaca ctggacatca aacgagactg
    29761 tctaagactt ggagtaccgt ttgaagatgt gtaccctttg caaacgacac ttttttaaca
    29821 ggagaaagaa catggcaagt aaaatcaatg cgacagaacg tattgctatc atcattgaga
    29881 aacaaaaaat agaggtcgtt acgactctaa actatgatat gagcattagc tttgataaca
    29941 aagacacggc tcctacacta gatgacaatg gtgacctttt tgaaccggtc tacaagtgca
    30001 aagttaaggc aattcccaaa aatgatgtat ttttcacctc attaacacga gtcaagagca
    30061 acatcaagac gctacaagag gttaaaaaat tctttgagtt cgtaaacgaa aacagagaaa
    30121 atctctttga gatggcagga tttaaggggg ctcttgaatg aaattgaccc tgaacattga
    30181 gcctaaacct caatcacgtc caaggtttgc aagacgtggg agttttacca caacttacga
    30241 agacaaggat atgaaaacat ggcgcaatca ttgccagctg ctcattgcta atcagtacat
    30301 gggtcagcct atccttgagg gagctttgag gacacggctt agattttata tcaaacctcc
    30361 tcagtacatt tctaaagtca agaagaacca acaggccctc ctggatgaaa ttataccagt
    30421 aggcaaaaag cctgacatag ataactacga aaaagcgcta tatgacagca tgtcagggat
    30481 cgtcttccag gacgacggtc agatagcgct acatgatgta ggcaagttct acagtctaaa
    30541 tccacggata gaggttgaga ttgaggtcat gaaatccctg agtatttgaa gaaatgagga
    30601 gcagatggct gactacgcat tatatcaagg tgatgtgttt gttacgcttg gaacattagc
    30661 gcagatcagt agcgagacag gaattactga aaggatgtta aagtattaca cttacacatc
    30721 acaccaaaga cgacacccaa acggtagggc cgttattaaa atcgaggagg aagataatga
    30781 gaattaagac ggaaagtgaa gaagttggaa gatgatggaa gagttaaagc aaaaagttaa
    30841 tgaagtatac aactggacgg tagaagacgg gaagccgcaa cctcccaagc aagatttacc
    30901 acaagcggtg aaagaccggg cggactattt ttgggaaatg gcagaagatg gtatgacgtt
    30961 tatgggagcg atggaatgca tcttcgctga tgaaaagcct acagactatg aattgggagc
    31021 tactaaggat tggttgccaa aatctaagga gtttgatgat tgggttggct attcgccaag
    31081 catggctcag gtagttattg cagtttattt gatttataga ggaaactaag atgaatgtac
    31141 agcgattaat tgagaagtat aaaaaacttg agggtgtatg ggatgctgaa ggagcagagc
    31201 tagctcgtca aatttttcta gaagacttga aacaactaga cgaatccgaa ataggttacg
    31261 cagatgaagc tccacgctac gtaaagaaca tactagcacg attgcgagaa ttgccattgc
    31321 atgatagaga ggtttggtta aaggctatca tgagcgaatt tgaacaggat tttagccgtg
    31381 caaaatggcg agagggctac gagcaaggta aaattgaggg tatggttgaa cgtgaaaaag
    31441 tcaaagttcc gcggtttgtg gctgaatgga ttgaggaagc tagaaaagct tgtaaagacg
    31501 tggtagaatt attcgaattt gatttcacga acgatgaagt tagaaaatgg tttatgcaag
    31561 aaagaccatt tgatttagta gctagagcat ggcttgatgg ctacgaggtc gaggaagaga
    31621 agcggtattt ggtgacttta aaaaataggc agcctttggt caaatcgcaa tcagggagta
    31681 ctctttattt tagtcaagat ataacagcta ggaattataa aggtactcaa aaagaactag
    31741 aagaagctgg cctcggatgg gtgttcgatt gtgaaggtgt tgagatagag gaggtggagt
    31801 gatgagcttt acgctaaata gcacaattgg agacttagtt ttggcaatcg gagaaattat
    31861 cgttggttct gatggtaaaa ccactacagc gatactggag atacctgatc aaagctttta
    31921 cttagagatt gagcttaaat tgaaggagga ggtcataaat tgaaacgatt catagctatc
    31981 tggattctgc tatctgctgg attgaatatc tggcagatgg ataggattcg agatttggaa
    32041 gagaagaagc cgatggttat ctacaaggct gataacgtag gtgctgagat atgaggagga
    32101 tttggcatga taccgaaatt tagagtgtgg cattatgaat taggtagact gatgtcagtc
    32161 aaatgtatgt tttttcagga tagcgagatt gaagaatttg agttaaacga tgctttaatg
    32221 aatgattaca ttacagctta tcctgacgaa atcgaactca tgcaatcaac aggactcaaa
    32281 gacaagaacg gcaaggaaat ctttgaaggc gatattgtac gaactactag atttttgggt
    32341 agagctgacg aaattggcgg tttctatgag tatgacaagg aatttatagg gattgttaag
    32401 cagcttgagg gttcttgggt aattgatacg ggcagtgacg cagtatgttt atggactgaa
    32461 attgaagaaa atgaaatcat cggcaacatc tatgaaaata aggagtttgg aggacgcaaa
    32521 tgagaccttg taaatatcca tattcaggaa gaagaaaaaa acaagaaacg ccgtcgccaa
    32581 tattttctgc acgaccaatt tttgacgaag ttccaattgt agaagaagtt aaggttgagt
    32641 tcggagttga agctagtatg gggcgcatat atccagaaac gttaatacat ttagatattt
    32701 ctggatacgg aaatagagtg cattcagtac atcgcttccc cggtatttta ctgagtgttg
    32761 gtgagtcaat ccaactaaag atgcttttct ataaaagact tagaaatttt actacagatc
    32821 gtttcttgac gtttagagaa tctgattgga agttctttat ccgggacctg gtcaacgaat
    32881 ttgtgcgata aaaaagccaa gacactctct gtctcggcta aattcctaat aagactatta
    32941 tatcacaaag gagacagaga gtgaacaagg ctaaagaact attgaaagag ttgcagaatc
    33001 tggacatgga cattcaaagc cgtatagatg aaattaacga gcttgaggca ggtttgctct
    33061 caagccccaa gtggattgac gtcaaagtcc aaggtggtca agctagaaaa gttgatgatg
    33121 tctatactca gcttgtcgtg atgaaagagg ccatagaaca ggatactaaa gaggttatca
    33181 acagaaagct tgagcttggt aggttgatta acaagctgaa aaatccaaag agcaggtcta
    33241 ttctcagggt gacttacatt actaagatgt atgttgatga tatttgtgac aaaatggaaa
    33301 tcagcagaac aactttctac acttggcgga atatggctat ctctgaactg aatgaggttt
    33361 tggagagaat ggaactaaat tgaactttac aaaaccgtac gggaaaaaat gatacttgtt
    33421 agcacagttt tgtaattctg ataaaatggt agtatcaaga attgaaaaga gaggtctcag
    33481 aattggtaga tggttacctg taatgtcagg gggctgtaat ggccttggag gttcaagtcc
    33541 tcccctctcc tttgagtgtt tgtgtcccag aatgagttaa atcttctggg tggggattca
    33601 catatcactc attaactcct atcactcatt aacttaaaaa tggttgcgga agcgactgga
    33661 cctcgcatga ttgcgtagct aattatattc cggataagtt ataagctaga gggtttgatt
    33721 ccctcagagg ttttaaatga ctacaaaaaa taaaaaaaga agtcaaaatt taatacgcac
    33781 gcaaggttgt agtcgccttg caagaaggtc gcacatcgtg tggctttttt tgattattcg
    33841 aaaggtggtg atggacattg ggttaaatca aagacaaaag atgtttgcga gcgagtattt
    33901 gaggactggt aatgtctatc aatccgcaat attcgcaggt tatagtgaag cgtatgctaa
    33961 aacaaccgct agtaaattgc tagaaaatgc aagcgttaaa acgtttatac aaaccgaaac
    34021 tgaaaagatg cacgatgaaa atattttgag cgctaaagag gctctttcga ttctctcaga
    34081 cattgcaaga ggccagcgac ttgaggaagt tttgatgatg aaccctgtca ctggtgaggt
    34141 ggatagagtt acgaaaaagg cagataataa cacagttatt aaagcgatag ctgagatatt
    34201 gaagcgttat ccgactgcta aacaagctga gaaactagaa cttgaaatcg aaaaacttaa
    34261 atcacaaatc ggtgtggata atgaacaaga cgataaattg atagactttg ccaaggcttt
    34321 gagaggtgct tttgatgaca aataaattta caaagcgaca agaagaagtg cttacacgag
    34381 tattgaatga tgattttttt atctgtggtc tccatggtgc aaaacgttca ggtaaaactg
    34441 ttctaaacaa catggtcttc atgaatgaga ttgcacgagt gagagaaaca gcggatagat
    34501 taaacataga tgagccgatg tatatcttag ctggaacatc ttcaacatcg atacaaaaca
    34561 atatcattca ggaactgtat aacatgtttg atattgaacc taaatacgat aagcacggag
    34621 cttttaccct ttgcggtgtc aaggtagttc aagtctacac cggttctata tctggtttaa
    34681 aacgcgcccg tggttttact gcttttggag cttatgtaaa cgaggcatct cttgctaacg
    34741 aacaagtatt caaggaaatc atctcacgtt gctcaggaga gggtgctagg attgtttggg
    34801 atagcaaccc agacatcccg acacactggc tcagacggga ttatatcaac tctggagacg
    34861 atatgatcat cgactttcat tttaagttag atgataatac attcatgtct gacagatacc
    34921 gagagaatat caagaatgcg acaccagctg gtgtatttta tgaccgagat attcttggtc
    34981 tgtgggtgac tggtgagggc gtcgtctatc gtgattttag cgagaatatg gttgtagata
    35041 acgtaccaga agatatcact aaagtctatg ctggtgttga ctggggatat gaacactttg
    35101 gctctattgt tgtcattgga gaaacatctg gcggttcagc ttatctgtta gaggaacacg
    35161 ctcatcagta caaagagata gatttttggg tagacctcgc taagaatatc aaggaacgat
    35221 acgggaatat tacgttctgg gcagacagtg cacgacctga acacgttgct agatttcaaa
    35281 gagagcaatt aagaacattc aacgctaata aagcggtatt gtctggtatt gaagaagtag
    35341 ccaagctgat gaaagctggg cgcttttttg ttgtatcaaa caaggtcagc aagttcaaag
    35401 atgaagttta tcagtatatc tggaatgaaa agacgggcga gccagtgaaa gagaatgacg
    35461 acgtgctgga tgcggtgcgt tatgcgattt actcacaaca ttcgcaacca aaagcaaccg
    35521 tccgcagacg ttctgattat ggtctataga gaggaaagac atgtaccaat atttaaccta
    35581 tccacgggat ggatatgatg agggttcttt gaagaaagac ctgatttaca aattgataac
    35641 gatacatagc actgaaagct cacatttgaa gaagcttaaa agctactatt tgggtgagca
    35701 tgctatctta gaacacacga gacgcaacgt gaacgcaccg aattacaaga cggtagccaa
    35761 tcatgccaag gatatcgcag acacggctac gggctatttt atgggcaatc ctatcaagta
    35821 taacaatact gctgacggtg atattgatga actacttaca gcctttgatg gtgctgagat
    35881 tgaccaagta gatgctcaga atgctttgaa catggctatc tatggtcgtg cttacgagta
    35941 catctatgct aaagagggta tggctgagtt ggattcaact agtattgatc cggagaatac
    36001 tttcatggtc tacgatgata gtattgagcg gaagcctttg tttgcggtct attactatga
    36061 agtaaaagac gatacgaaag acactaccaa gcaccaggct gaggtcttta ccgaaaatct
    36121 gcactatcac atggtgctga gaagtacaga ttcaggaaca actcagagcg aggaggcaac
    36181 acctcacaac cttggtcaaa tcccaattat cgagtatcgc aataatcact ttgcgattgg
    36241 cgactatgag caacaaatta gcttgataga cgcttataat tccttgatgg ggaatcgtgt
    36301 caatgataag gaacaggctg tagagtctat
//
LOCUS       DAVMAF010000012        35932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMAF010000012 DAVMAF010000000
VERSION     DAVMAF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.012"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(116..274)
                     /locus_tag="VYO97_000602"
     CDS             complement(116..274)
                     /locus_tag="VYO97_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1753447.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(345..791)
                     /locus_tag="VYO97_000603"
                     /pseudo
     CDS             complement(345..791)
                     /locus_tag="VYO97_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(867..1457)
                     /locus_tag="VYO97_000604"
                     /pseudo
     CDS             complement(867..1457)
                     /locus_tag="VYO97_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1381..1575)
                     /locus_tag="VYO97_000605"
     CDS             complement(1381..1575)
                     /locus_tag="VYO97_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1753448.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
     gene            complement(1808..2095)
                     /gene="tnpB"
                     /locus_tag="VYO97_000606"
     CDS             complement(1808..2095)
                     /gene="tnpB"
                     /locus_tag="VYO97_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1753449.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWLSTEKDVKALAPEQVD"
     gene            complement(2076..2261)
                     /locus_tag="VYO97_000607"
     CDS             complement(2076..2261)
                     /locus_tag="VYO97_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753450.1"
                     /translation="MPQPIVPVEIPQSRLFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2565..4127)
                     /gene="guaA"
                     /locus_tag="VYO97_000608"
     CDS             complement(2565..4127)
                     /gene="guaA"
                     /locus_tag="VYO97_000608"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1753451.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4269..4967
                     /locus_tag="VYO97_000609"
     CDS             4269..4967
                     /locus_tag="VYO97_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1753452.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5010..5906)
                     /locus_tag="VYO97_000610"
     CDS             complement(5010..5906)
                     /locus_tag="VYO97_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1753453.1"
                     /translation="MSNSLKGTLLTVVAGIAWGVSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMILAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQANIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5915..6850)
                     /locus_tag="VYO97_000611"
     CDS             complement(5915..6850)
                     /locus_tag="VYO97_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1753454.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6847..7572)
                     /locus_tag="VYO97_000612"
     CDS             complement(6847..7572)
                     /locus_tag="VYO97_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1753455.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(7656..8291)
                     /locus_tag="VYO97_000613"
     CDS             complement(7656..8291)
                     /locus_tag="VYO97_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1753456.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8293..9120)
                     /locus_tag="VYO97_000614"
     CDS             complement(8293..9120)
                     /locus_tag="VYO97_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1753457.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9142..10459)
                     /locus_tag="VYO97_000615"
                     /pseudo
     CDS             complement(9142..10459)
                     /locus_tag="VYO97_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189734.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10707..11318
                     /locus_tag="VYO97_000616"
     CDS             10707..11318
                     /locus_tag="VYO97_000616"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1753458.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11363..12091
                     /gene="rlmB"
                     /locus_tag="VYO97_000617"
     CDS             11363..12091
                     /gene="rlmB"
                     /locus_tag="VYO97_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1753459.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12130..13041)
                     /gene="trxB"
                     /locus_tag="VYO97_000618"
     CDS             complement(12130..13041)
                     /gene="trxB"
                     /locus_tag="VYO97_000618"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1753460.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELSEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLNPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13107..13331)
                     /locus_tag="VYO97_000619"
     CDS             complement(13107..13331)
                     /locus_tag="VYO97_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1753461.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13409..14152)
                     /locus_tag="VYO97_000620"
     CDS             complement(13409..14152)
                     /locus_tag="VYO97_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1753462.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14152..14952)
                     /locus_tag="VYO97_000621"
     CDS             complement(14152..14952)
                     /locus_tag="VYO97_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1753463.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15110..15466)
                     /locus_tag="VYO97_000622"
     CDS             complement(15110..15466)
                     /locus_tag="VYO97_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1753464.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15479..15991)
                     /locus_tag="VYO97_000623"
     CDS             complement(15479..15991)
                     /locus_tag="VYO97_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1753465.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15991..16485)
                     /locus_tag="VYO97_000624"
     CDS             complement(15991..16485)
                     /locus_tag="VYO97_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753466.1"
                     /translation="MEIRLAFPNEVGAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16620..17066
                     /locus_tag="VYO97_000625"
     CDS             16620..17066
                     /locus_tag="VYO97_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1753467.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGINPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            17063..17710
                     /locus_tag="VYO97_000626"
     CDS             17063..17710
                     /locus_tag="VYO97_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1753468.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17731..18312)
                     /gene="pdxT"
                     /locus_tag="VYO97_000627"
     CDS             complement(17731..18312)
                     /gene="pdxT"
                     /locus_tag="VYO97_000627"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1753469.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18313..19188)
                     /gene="pdxS"
                     /locus_tag="VYO97_000628"
     CDS             complement(18313..19188)
                     /gene="pdxS"
                     /locus_tag="VYO97_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1753470.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19340..20719)
                     /locus_tag="VYO97_000629"
     CDS             complement(19340..20719)
                     /locus_tag="VYO97_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1753471.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            21013..21936
                     /locus_tag="VYO97_000630"
     CDS             21013..21936
                     /locus_tag="VYO97_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1753472.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21996..22601
                     /locus_tag="VYO97_000631"
     CDS             21996..22601
                     /locus_tag="VYO97_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1753473.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22620..23864
                     /locus_tag="VYO97_000632"
     CDS             22620..23864
                     /locus_tag="VYO97_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1753474.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(24087..24344)
                     /locus_tag="VYO97_000633"
     CDS             complement(24087..24344)
                     /locus_tag="VYO97_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1753475.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24386..26422)
                     /locus_tag="VYO97_000634"
     CDS             complement(24386..26422)
                     /locus_tag="VYO97_000634"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1753476.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26682..27599)
                     /gene="glyQ"
                     /locus_tag="VYO97_000635"
     CDS             complement(26682..27599)
                     /gene="glyQ"
                     /locus_tag="VYO97_000635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1753477.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWANGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(28320..29081)
                     /locus_tag="VYO97_000636"
     CDS             complement(28320..29081)
                     /locus_tag="VYO97_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1753478.1"
                     /translation="MDNLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSSFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEYFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTIDKLVENLG
                     SVQAVFDSYHLWANTDKTLPLLNWFVQQKLIEKEI"
     gene            complement(29354..30196)
                     /locus_tag="VYO97_000637"
     CDS             complement(29354..30196)
                     /locus_tag="VYO97_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1753479.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(30310..31701)
                     /locus_tag="VYO97_000638"
     CDS             complement(30310..31701)
                     /locus_tag="VYO97_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1753480.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTPFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31939..32403)
                     /locus_tag="VYO97_000639"
                     /pseudo
     CDS             complement(31939..32403)
                     /locus_tag="VYO97_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            32557..33534
                     /locus_tag="VYO97_000640"
     CDS             32557..33534
                     /locus_tag="VYO97_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1753481.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            33531..34613
                     /locus_tag="VYO97_000641"
     CDS             33531..34613
                     /locus_tag="VYO97_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1753482.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(34677..35724)
                     /locus_tag="VYO97_000642"
                     /pseudo
     CDS             complement(34677..35724)
                     /locus_tag="VYO97_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10621 a         7991 c         6508 g        10812 t
ORIGIN      
        1 aattggagaa tgatatggga ttttcatata ataaattgta accgcccaat aacaaagtct
       61 attgaaaaat ctccagacta gagaactcat atatagttcc taatctggag atttctcatt
      121 tgcacttttc ttgtacaact ttagtccatg gtaaataggc ctctaaaacc tctttgttta
      181 cgagagtttc ctcgtttgga agacattcta gaagatagga tagatatttc tcactattta
      241 gttgatgacg tttagctgtt tccaacaaac tcataataat agctcttgct ttagctcctt
      301 caaaactttg agaaaataac caccctcgct tttttaaact gagcttaggc taaaagagtc
      361 cactggactc ttttactccg tcccataacc aatgatttaa tggcgcattc agctagatta
      421 ttggaaagga ccagatgtcc gtctttcaaa atggtcttaa aggtttcttc atacttgagg
      481 ctgtattcaa ttgcccttcc tagttttgaa cctgctaaaa ctgactgacg gcggcaccaa
      541 gcaaagaagt cttctattag gggttggagc tcttcttgac gtttctgtag tcgttcatca
      601 gctggcaaag cctcccagtc tctttccaag gaaaataact gatcacaata ggctaatcct
      661 ttagctccta aagatgattt atctgcttgc ttgggggtcg cttcaaaaaa cttccttctc
      721 acatgcgccc aacatccaac aagtgcagct tcttccagtt gacgataagc ttctagcggc
      781 tcgcagttta ccaagcttag cgtcgccttg ctcctaagcc ttggactgct atcgcataga
      841 cagaactaga ggactaaagt cctaagttac tgccgcaaca tatcacaatg cacatagcca
      901 gaataatctc ctaggaattc ttgtactact aaaccactcc gacgctgatc atggtggtac
      961 agcgtgattg cttgattctc agctttccca gacaaaaagg tccaatagta ggtcagctga
     1021 ctatcactct ctagcaccct ataagaagtt tcatctgcat gaagtaaggg cttgagtcaa
     1081 tagtctcgtt ataaaggggc tttaggaata ggagctttca cgattttatc actcggattt
     1141 ttatcactgc atgcttggca tttgtaagcg tgttggatat gatctatgcg ttttaattgc
     1201 gcaggaataa agaccaattc ttgtccttga agactccctc caatctcttt tagatctccc
     1261 tgacaatcag ggcaaatcct ctcttctact tgatgatgaa cttcttctga atcaaattgg
     1321 gcaagaagag cttggcgttt ccctttagat ttcttacgtt tgtaggtaat ttcctctgtt
     1381 tcaactggga acatctccat cttcctcaga tggagactct tcctcaaaaa ggttgagttg
     1441 tccagagggg caaacacttt tctcagagga ttttccatag agcttttgag tcagataagc
     1501 cacttgttca cgcagaaggg caagttcatt ggtgagacta tcaattgtag cactctgttg
     1561 ttgaatgatt ttcaatcttc ttccatgagc ttccctctct gttttctaac tttattatac
     1621 taaaaagaaa gtcctcattt caatagaaat cacgactttc tgatggattt atagtaaaat
     1681 gaaataagaa taggacaaat cgatcaggac agtaaaatcg atttctaaca atgttttaga
     1741 agcagaggtg tactattcta gtttcaatct actatatttt tggagtgata gaaaagccct
     1801 tcataagcta gtctacttgt tcaggtgcga gagctttgac atccttttct gtacttagcc
     1861 aagtcagtct gccgttctca aagcgtttat atagtagcca aaatccttga ccatcccagt
     1921 aaagggcttt aaagcggtct ttacgtccac cacaaaagag aaaaacttga cctgagaaag
     1981 gatctaattc aaagtgggtt ttaaccagat aagccagtga atcaatgcct tgcctcatat
     2041 ccgttttccc acatacgaga tagacctgtc ctaggctaga tagatgaatt gtcatagagc
     2101 aacaccttat ccaaaagctg ttctaccatt tcgagattga gagattgaaa aaaacttact
     2161 tcaagcttgc caatacgaat tttaagcaga atatcatttc tctttttaga atcaaaaaga
     2221 cgagattgtg gaatctctac aggaacaata ggttgtggca ttaaaatatc ctccaatagt
     2281 tttactttac taatagtata caggaggagc tccatgaatg atagatacct tgttatgacg
     2341 cgcttactat attagaaagt tggctctcta attaaattaa ttctcattat atatatcaaa
     2401 agaccgcagc ttgaaaacta cggtctaatt ttttgtccaa atgtaaacca cttggaacta
     2461 ttattcaatt tattcttttt agtaaatgtt cccgttttaa tcccagttac agtttttaat
     2521 cctctgtaac cgttgatttt acaaagcttt taattttttt gcgattactc ccactcaact
     2581 gttgcaggtg gtttacttgt aatatcgtag acgatacggt taacatgatc cacttcattt
     2641 acgatacgta ctgagatttt ttgaagtact tcccatggaa ttttggcaaa atcagcagtc
     2701 ataccatcga tagaagtgat agcacggatt gcaatcgtgt agtcatacgt acgaccgtca
     2761 cccataacac cgactgaacg aacgcctgtg ttaacagtga agtattgcca aatatcgcgg
     2821 tcaagtccag ctttagcgat ttcttcacga agaatagcgt ctgattcacg aacggtttca
     2881 agtttctctt cagtgatttc acccatgaca cggatagcaa gtcctggtcc tgggaatggt
     2941 tggcgccata cgatatggtc tggcatacca agctctgtac caagagcacg aacttcatcc
     3001 ttgtaaagag tattgagtgg ttcaatcaat tcaaactgca tatcttctgg aagaccaccc
     3061 acgttgtggt gtgacttgat agtttgagct gtatccgtac cagactcgat aacatctgta
     3121 tataaagtac cttgagcaag gaatttcaca tctttgagct tgcttgcttc gtcatcgaat
     3181 acatagacaa actcgttacc gatgatttta cgtttttgtt caggatcaga aacgccagca
     3241 agtttgtcaa ggaaacgttt agcagcgtct gctttgacga tattcaaacc aaacttacca
     3301 ccgagcatgt ccataacttg atcagcttcg cctttacgaa gaagaccgtg gtctacgaag
     3361 atacagatca attgatcgcc aatcgctttt tggagaagaa ccccaacgac agatgagtca
     3421 acaccacctg atagaccaag aaggacacgt ttatcaccga cggtttcacg aatttttttg
     3481 atctgcatgt caatgaaatt atccattgac cagtcacctt tagccttaca aatgttaagg
     3541 gcaaagttac gaaggatatc atttccgtat acagaatgac gaacttctgg gtggaattgg
     3601 ataccgtaaa tgtgtttatc tgggttttcg atggctgcgt atgggcagtc agctgatgta
     3661 cctgtacgaa caaagtcagc aggaatctca gtaaccgcat caccatggct catcaaaaca
     3721 gtctgttcat caggtgttga ttcaaaaagc gctgatggtg tgtgagttag ggttgattga
     3781 ccgtattcac gatttccagc atcacctgca ggaacaactt ttcctccaag tttatgggtc
     3841 aataactgca taccataaca gattcccaaa attggaattc cgagttcgaa gatttctggg
     3901 tcaatatcaa atgaaccatc ttcatataca gaatttggac cacctgatag aataattcct
     3961 acaggattga cttcacgaac ttcagcagct gaaattttat ggctttttag ttctgaaaaa
     4021 acaccaatct cacggatacg gcgtgaaatc agctggttgt actggctacc atagtccaat
     4081 acgatgattt tttctacatc ttgcaaatca gttgaaatgt tgctcatctt tttcctttct
     4141 caaaagaaat atcttttctc aatattctat cacaaataag ggcgaattac caactaaaca
     4201 aggcgaagtt tgactttttc tagtttattg gggtacaata gagaaaagat agaaatgaag
     4261 tgaaaaatat gctacctgct tatatgaaaa tccatgatca gataaaaaag gatattgacg
     4321 agcaccgttg ggctattggt gagaggcttc ccagtgaaag agatttagct gagcagtttg
     4381 cggtcagtcg catgaccctc cgccaagccg tatctctatt agtcgaagaa ggcgtcttag
     4441 agcgccgtgt aggaagcggc acctttgttt ccagtactcg agtacaagaa aagatgcgag
     4501 ggacaaccag ttttactgaa attgtcaaat cccaaggtaa agttccctct agccagctca
     4561 tttcctacaa aaaaaccatt cccaatgagc aagaagttgc caagctagga atttttccaa
     4621 cggacaatat tatccgaatg gagcgggtcc gctatgccga ccaagttccc ctagtttatg
     4681 aagttgcttc tattcctgaa aaattcatta aggactttaa aaaagaagaa atcaccagtc
     4741 atttcttcca aaccttgcaa aaacatggct atcgtatcgg caaatctcaa cagaccatct
     4801 atgctcgcct tgctaaagaa aagattgccc actatttgga agttgaaaaa ggacatgcta
     4861 ttcttggatt gacacaggtt tcctacctag aagatggtac tgcttttgaa tacgtaaaaa
     4921 gtcaatatgt aggcgaacgc tttgaatttt atcttgaaaa taattagatt accacgcaaa
     4981 aactctctgt cacagacaaa gagtcttttt tatttttcta agaataaatc tttcaaagaa
     5041 atcaaagtta cagcaaacaa tatcattgcc ataccaagaa aatcaatggg ataaaattgt
     5101 tcattcatta ttaagaaggc aaagaaaacc gccgatattg gctcaattga agccaacaag
     5161 cttgacttga ccggtcctat cagactggct cctttaagga aagctgtata ggcaaagaca
     5221 gtcccgataa ggataatacc tgcaaacgca aggagaaaat caagactagt cgggatattg
     5281 gcctgtagaa cccctgtaaa aggaagggcg accaaacctg ctataaccat tcccacacca
     5341 atgaccaagc tgctccccca ctttttaatc aaggctatgg gtaaaatgat atatagagca
     5401 taagtcaagg cagaaaagag accccagaac aggccagcag gtgtcatgga taactggtcc
     5461 aactgcccat gtgttgcgat caggaaggtt cctccgatgg cgaatatgat ggaaactatc
     5521 tctcccagtg tcggtgccac cctatccttg atacagctat aaattaaaat tccgacagga
     5581 caaacatact gaagcaccgt cgctgttccc gcattggtct cctgaatagc agacagatag
     5641 gcgaattggt tgagaaaaag accaatcaga gcaaaaataa gaagagacag caaactcttt
     5701 ctatccttta aaaagaccag tattttatcc tttgcagtag cataagccaa gatcatgaga
     5761 attccaccag cgattaaaag acgcaagttg gtcaagacca gagccgaaat tccgtgtgcc
     5821 attaggtatt ggccactcgt tcctgacacc ccccaagcaa taccagccac aactgttagt
     5881 aaagtccctt ttaaactatt tgacatgtct ctccttactc ttctttacga atcaagttca
     5941 tcacttgatt gcggtctaat atccattgag ctctctcgtc cttttcatag gtacgattcg
     6001 ataggaaaat agtggcttct tgcttctgac gattccacat gataaaggta cctgtatagc
     6061 ccgtatggtc tagccaatct ccttccaaat tccatgctaa agaacgttcc ttgtcatcca
     6121 aaggagaaaa attttgattt aagtctcttg caaaatcatc tgctaaatag tgttctaaaa
     6181 agatttgtaa atcctttata gtcgaaaata aaccagcact cccagcatgt ctacccagga
     6241 gacgagcctt gggatcatgc actatgcctg cctctacacc tctaactgtt ggaacagcaa
     6301 gctcaactgg cccaaacttg gtttccgtca ttccccaagg tttccagact tgatccttta
     6361 aaatcacatc caaatcttga ttaaaaattc tttccaaaat aaagcccaac agcaaaaaat
     6421 ggacatccga ataaagaaag gctggctgac ttcgtctgtt gagatgaaac atcgcttcct
     6481 ttaattcagg ggctgttaaa agatcacgat taggaataaa aggatcaagg tctgttgcat
     6541 gagtcaagag ctggcgaata gtgatgtctg gataatcact ctcaggtaaa aaatctatta
     6601 ccagtctatc aatatctaat tgacctattt cccacaagaa ggtacaaact gtgccaaccc
     6661 caacaacctt gctgacacta gctaggtcat aaactagtcc tgcctcagtc tgcaagccat
     6721 gctctgggtc actctggcct aaatagaact ctgtccattg attgtcctta aaatacgcaa
     6781 aagaggctcc gggataaatt cctgcctcga tttgttcttc tattttttta ataatcttgg
     6841 tccacttcat acttcttcaa accacaattc aacattattt ctatctttac aaaggaagaa
     6901 tttttcagac ttaggaataa aatattcagt agactcaaac ttctggcgaa gacttgctat
     6961 gtctaattca ttgaccaaga acttgagcat agataagtcc caggtaaccg tattgtccac
     7021 agtcaaatcc tgcccctgag ctggaataaa atcaagggat gccccaattt cagatgattc
     7081 caagaaactt tcgatatcag ttgggagatg taattccata gaaatctcaa atttacttaa
     7141 agaaattgat gccaaatctg tttgaaattc aggcttttct cctacttcta ctagacttgc
     7201 tatgtcatct tccgcatgaa tcaaaatcaa atcatcttct ggtgagaaaa tttcaaaagc
     7261 gtagccattt tgacctttat ataatcgatg aatcgaatct gttttagata agattccttc
     7321 aatttctaag ggattttcca ccttgacaat caatctagct agtttttttc ttccctctac
     7381 cttacgagta cgcatactgg gagcttcttc taaaaccagc ttttcaagac ccgtttggtc
     7441 acctagtgac agaaaggccg attcttctaa caaggccttc attccaaggg tttcaatata
     7501 aaatgtttca tttaattttc tattattagc ttttaaagta ggaataatcc gtacaatctg
     7561 atttacattc ataaattcct ccgtcacttt ttattttaaa ggattttaga attttttaca
     7621 agatattatg ctcaaattta acattattta gctatttagt aaagatagtc tttcaaggat
     7681 tttgacaaag cctgataacc agcaatactg aggtgcagtc catcagttgt atattctttt
     7741 ttgagttggc ctgcttggtc tgtcaaacaa tcaaatactg gcacaaattc cacctgcata
     7801 taggcagatg caagctcttg ataggcttga ttccagttct gaattttttc attcgagcgg
     7861 atatagactg cctgctggta ctcctctccc tcattgacag gcaaaatgga aagcaattta
     7921 atctctgtca atggataatc gcgagcaacg gattgaatga tagcttcgag attattgaga
     7981 gcctcattca caggaacatc ctttccgata tcatttgtcc caatcagaag aaaaatttta
     8041 tctactgctc caccatatag atgagcatca aggttctcta gtaacagtcc tgtctgatag
     8101 ccacgaattc ctcgattgac aatcgtcttt gaagtcccaa atagctcctg tagaggataa
     8161 tactcgacaa tggaatcccc aataaaaaga atgtttggtt ctacaacaga aatgtgattt
     8221 aggtgacgat acttggtttg aattttttct tgttccttta ggagccaatt ttctaataac
     8281 tgtactgcca cttcattctc ctttttctaa ccagttttcc aagacttgat aaactcctcc
     8341 ttggctgttg gctggtgcta gagcagtcgc cacagctttg gctttctcat cagcattttc
     8401 catcgcatag gcaattccag ccatttcaag catttcaaca tcattttcac tatcaccaaa
     8461 agccatgatt tcttgggatt tcaagtccca gcgcttgagt aattcctcca agccccatgc
     8521 tttatgaatc ccagcttgga ggatatcaat gcaaccatag ccactggata cagctcggac
     8581 acggccatca aagagagtat tgatttcttc caaaaccgaa ctcaaacgtt cctcaccaac
     8641 aaccatgctc atcttgagca caccaccaaa gaggtcagat gttaattcat ccacaaattg
     8701 catccgttgg tagaattttt caatcatttc tggagtcata aaactttcaa gatctgtaaa
     8761 aatcgtacct tccttgacaa aatcaccctt catccccgtt acaacaaact ggtcctgaca
     8821 cgctcgaccc ttgaaatgag ccaaagcctt gttgacaatg gcgtcatccc atgtctgagc
     8881 ctgaatcaat tcattgtttt caaaaatacg agcgccatta gcaacaacca gaaccactcg
     8941 atccaccaag ggactcagta gttgtctcat gcggtgaatt tcattgcccg tcgcaatgac
     9001 aaaacgaatg cccctttgat ccaactgatc taaaatcttt tccaatcgtg ggagatcaag
     9061 ctgacctcta gcatccagca aggtcccatc catatctgtc gcaattacct taatcatcat
     9121 ttactttcct ctggattcaa gctaatacca cttcaatccc acatgttcat tcatttcttt
     9181 ataggctgta tcaattcgtt ccttagtcgc ttcatctaac tggtcacgat gactgccatc
     9241 ctctataaaa tcttccaaaa tataagtttg gtaatgatac aagggatatc cttctggtga
     9301 aaaggttccc tttggtgttc gattgaccca agtaggatga tctttagctg ttccgatagt
     9361 tgtttttcca tcattcttct tgatggtgac atccatgaga acaccacgtt cagtccactt
     9421 agcattctct tcatctccca tagattcaat tcgttgattg gaaatgaagt tccccattga
     9481 atagataatg agtttcttat ctccatcttt ttcaaccgtt tcagatggtt caacaacgtg
     9541 aggatgccct ccaaagataa tatccgctcc ccaatcgatc atcttgtgat aaagagcttt
     9601 ttgttcttca gttggttcca atcgatactc aacacccatc tgaggcatga taatggtgat
     9661 atctgcttcc ttctctgccc gttcaacttc agccttcatc ttatcttcgt ttaaatctaa
     9721 aagataacga ttatagtctt cctgagaaat atactgctca attccattga aaccatagga
     9781 ataggctgac aatgcaacct tgataccatt cacttcctta atgaccagcg gaacctgatc
     9841 acgtggttcg tgcgtataaa ctccgattgg agtgattcca gctttctcaa taatatcggc
     9901 cgttgaaata actccctcaa tttgcgaatc caaaatatga ttatgagcta aatccagcac
     9961 atgataacct gcctccttaa tagcatccat aacttcagca ggagcattaa agagaggata
    10021 acccgctaaa taatgatcct tattaatggt ttcttcaaaa tcaccaatag ctaaatctgc
    10081 ttgcttgaac caaggagtca catactcaaa attttcatga aagtcatagg taccgtcttc
    10141 ttttttagct gaaaagaaaa gtccatcgtg gtagagcaaa tccccgtggg ccataattct
    10201 ggcagttttt tcctcttcct actcctgaga cttttgctta gttccctctt gagaaatagt
    10261 atctcgtttt tgactagtaa agagattcct tggaagcttt caaacaacaa gaccaagccc
    10321 attgataaac caactgctag caagagtatc gccacaaatc ccttattgct ccacttgcga
    10381 taactcctaa aaagtttacc aagcccttca taaaacgaaa agctaaacca ccctgatttc
    10441 gattttgtct tctttgtatc ttcgttctcc ctactttctt atgcaagcct tttcttttta
    10501 ttatatcaca gataagtatt tctttcacaa ttgaattgaa cttcccatct attttctata
    10561 aatcctaaat gccataatgc tttcaattct tgtcattttg tgatatcatg tagaggaaat
    10621 gaactaatcc acagtggctt attccaagta taccacttgg gctttggcag tagctaactg
    10681 cactaaacat aatataagga gagaaaatgt ctgcaataga acgtattaca aaagctgctc
    10741 acttaattga tatgaacgat attatccgcg agggaaatcc tactctacgc gcgattgctg
    10801 aggaagtcac tttcccccta tctgaccagg aaatcatcct aggcgaaaag atgatgcaat
    10861 tccttaaaca ttcccaagat cctgtcatgg ctgaaaaaat gggactccgc ggtggtgttg
    10921 gactggctgc tccccagtta gatatctcaa aacgcattat cgctgttttg gtacctaata
    10981 ttgttgaaga aggcgaaact ccacaggaag cctacgattt ggaagccatt atgtacaatc
    11041 caaaaatcgt ctctcactct gttcaagatg ctgctcttgg cgaaggagaa ggttgcctgt
    11101 ctgttgaccg taacgtgcct ggctatgttg ttcgccatgc ccgcgttact gttgactact
    11161 ttgacaaaga tggagaaaaa caccgtatca aactcaaagg ctacaactcc attgttgttc
    11221 agcatgaaat tgaccacatt aacggtatca tgttttacga tcgcatcaat gaaaaagacc
    11281 catttgcagt taaagatggt ttactgattc ttgaataaag aaaatcccgt tgcaagacgg
    11341 ggttttgtgt tataatagag gcatgaaaac aaatgatatt gtctatggtg tccacgccgt
    11401 taccgaagct ctccttgcaa atacaggaaa caaactctac ctccaagaag atctccgagg
    11461 taagaatgtt gagaaagtca aggaactagc tacagaaaag aaggtgtcca tttcttggac
    11521 atcaaaaaaa tctctctctg agatgactga aggtgctgtt catcaaggtt ttgttctacg
    11581 agtgtctgaa tttgcctata gcgagctaga ttacatcctt gcaaaaacac gccaagaaga
    11641 aaatccactt ctattgattc tagatggtct aaccgatccc cataatctgg gttctatctt
    11701 gcgaacagcc gatgcgacca atgtttcagg tgtcatcatt cccaagcacc gtgctgtcgg
    11761 agtaactcct gtcgttgcca aaacagccac aggtgctatt gaacacgttc caattgcccg
    11821 agtgacaaac ctcagtcaaa ccttagataa actcaaggat gaaggcttct ggacctttgg
    11881 aacggatatg aacggtactc cttgccacaa gtggaataca aaagggaaaa tcgccctcat
    11941 cattggaaat gaaggaaaag gtatctctag caacatcaaa aaacaagtcg atgagatgat
    12001 taccattccg atgaatggac atgttcaaag ccttaatgcc agtgttgctg cggccattct
    12061 catgtacgaa gttttccgaa atagactata aaaaagtttc cagtcatctg attggaaact
    12121 tttttatgat taactatgtt ctgtgataaa cttgtaggct tcttgaccag cgatagctcc
    12181 atctccaact gctgttgtta cttggcgaag gtctttcaag cgaacatctc caactgcaaa
    12241 gataccgtcg actgcagttt tcatgtggtt atctgtcaca atccatcctg cctgatcttg
    12301 gatattcaat tctttaacaa aatcgctaag agggttcaaa ccaacataga taaagacacc
    12361 accgaaggct tgttctgtca cttgacctgt tttcacattt tcaaatacga ctgattctac
    12421 tcggttttca cccttgattt cccttactac agaatcccag ataaagctga ttttttcatt
    12481 cgcaaaggcg cgatcttgta aaaccttttg ggcacgaagt tggtcacgac ggtgaacaat
    12541 ggtaacagtc ttagcaaaac gagtcaagaa gagggcttct tcaacagctg aatctccacc
    12601 accaactacc aataaatctt ggtcacggaa gaaagcacca tcacacacag cacagtaaga
    12661 aacaccacga ctgttcagtt cttcttctcc aggcactccc aaaggacggt gtttagaacc
    12721 agttgctacg ataactgtac gtgtttcata tgtttggtca tcagtcatca ctttcttaaa
    12781 atcaccatgg tcttcgacat tttcaacata accataaatg tgctcaacac caagattttc
    12841 aagtggttca aacatctttt cagacaattc aggtccacta atattagcgt atcctgggta
    12901 attttcgata tcagatgtat tattcatctg accacctggc agaccacctt caatcaaagc
    12961 tacttttaga ttgcttcgag cagcatacaa ggccgcagtc atccctgcag gtccagcacc
    13021 gataataata gtatcgtaca tatagattcc ttctttcttg ttgtaactat ctttattcta
    13081 actctttctt gtcaatcacg caaggattat gccttgaaaa caagaattaa actcataacc
    13141 gcaaaggata atactgggac aaccaagact ccaatcataa tcatatcata gagatgggct
    13201 tggcgagccc tggctgtctt atcaactccc gacatggctc tcagaccaat ccaaatccct
    13261 aaaaaaatca gaacaaggat ggtggtcaag atcaaactct cgaaatataa agaaaatagt
    13321 tgcagtagca tgatttctct catttctatc ttttttaaag agtaaactca gctagtccaa
    13381 ctaactgagt tttcctttat ctattatatc aaatataagt ccgtttgtaa ctagcgaaga
    13441 attcttttgt ccgctcttct ttaggggtgt ggataatctc atccggagtt ccagactcta
    13501 tgattttccc cttatctaag aagagaattt tatccgcaac ttgggctaca aaggacatgt
    13561 catgactgac caaaatcatg gtctgacctg acttagcagc atctgcaata gacttttcta
    13621 cttcaccgac caattctggg tcaagggctg aagttggttc gtctaagagc aaaacatctg
    13681 gtttcatagc aagcgcacgc gctagggcaa cccgttgctt ctgtccacct gataaatggc
    13741 gaggataatg gttttcacgg tccgaaagcc caaccttagc caactcttcc ttggcaatct
    13801 tagtcgcttc ttggtcagat aatttcttga caacaaccaa gccttctttc acattatcaa
    13861 gtgctgttcg gcgttcaaac aaattaaact gttggaaaac catagacaac ttacgacgta
    13921 gggcaaggat ttcttcttga gtgattttag aaaaatcaac tgaaaaacca tcaatctgaa
    13981 tagagccact gtcaggtgtt tcaagataat tgagactgcg aagaaaggtt gattttccag
    14041 ctcctgaaga accaatcaag gctacaactt cccctttttg aatatccaag tccagatgat
    14101 ccaagacagt ctgtcctgaa aaggatttgc ttaaattcga aatcttaatc attaacgaag
    14161 gtctcctttc acatctgttt gcactgtatc aggtgcagaa atagccattt ttctctcgat
    14221 gaaacgaccg aggctttcaa ttccgatatt gactacccaa taaacaaggg caacggagat
    14281 gaagcgttca aaatagcgat aatcagctcc acctagaatc tgagcttggg caaagacttc
    14341 cacaacaccc gcactaaaag ctagagatgt tcccttggtc aaaccgatga gggaattaat
    14401 caaggttgga gtagctacca ccgctgcatt aggaataatc actcgtcgat aaacttgcgc
    14461 tcgggtcata cccagactgc gtgccgcctc aatctcacca ggattaactg agagaatggc
    14521 tgcacgaatg gtttcactag cataagctgc ctcattaaag gcaaaggcga caatcgcaaa
    14581 agctgcagct ggaatcgcat tgatattgag accagttccc cattgctgat tgagggcttt
    14641 caaagccaaa gggattccgt agtaggtcaa catgagttgc accaaaatcg gtgtcccttt
    14701 taagaaacta acaaagaagg cctgcaaggg atataaaatc ttgacacgat tgatcttcac
    14761 aatggcaaaa agaagcgcca aaaccaagcc aaaaagggca ccaccaattg tcaacataat
    14821 tgttgttgga agttgttgga caattctagg gattccatca aagaccgaac gtaggctaaa
    14881 cagcttgcca tccggaatca attgcatcaa gttttggtac caatctgatg ctaaaatcgt
    14941 tgtaacattc ataaaaacat cctcttctga taatgattca ttctaccatg cttctgccgt
    15001 caatgaaatt atttgctacg gtcagataca gactttgtct acctactagt ttatcatact
    15061 ttgaaacagg gaagttatat attttatcta tcaaagagat agataaaaac tatttcagtc
    15121 ccagttcttc ataagatttt cgataaccga tttgcttaac agttccattt tctactaaaa
    15181 tgggccgttt taacaacata ccgtcacttg ctagcaactc agccgcttct tggtttgaca
    15241 aacttcctac cttatctttt agccctaatt cacggtattt gataccactg gtgttgaaaa
    15301 attgcttcaa ttcaaatcct gaggtttcta gccaattcaa aatgacttct tggctaggtg
    15361 tttcttccac gatatggacg gctttatagt ccacacctaa ttgatttaat tcttgttttg
    15421 cttttttaca agttgaacat ttggggtatt cgataaattc taacatgtgc ttctccttct
    15481 atttgtttat atctttaaaa tctacgcctt catgctccaa gagccaagct ttcttttcca
    15541 ctcctgcagc atagcctgtc agacgcttgc ctgctcccaa cacacgatga caaggtacta
    15601 ggatagacca aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca
    15661 ggtcttgagc aatttgtcca taggtcactg tctgaccata aggaatgccc tgtaaatagt
    15721 cccaaactct cttttcaaaa ttcgttccga ttggcgccaa gagcaagttg gataaatcct
    15781 gaggcttgcc tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac
    15841 taacaacttc ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa
    15901 cccaaattcc atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg
    15961 agtagaggat ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg
    16021 ttcgccatct actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg
    16081 cataaccttg ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat
    16141 caagccctct aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat
    16201 acggtgaaag actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc
    16261 ataggcctcc tctggactct tggtcacagc agcataggct agcagttctc cctcttccaa
    16321 ggctacgtag gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc
    16381 attttgccac tggttactac cagcatctgc taaacatttt ttggcatcct ctatcacctg
    16441 catgatggca cctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt
    16501 ctgactagtt tctcccatca tagcataatt taagtctttc tacaagcagt taaaacttga
    16561 ctttgtattt ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa
    16621 tgaaaactac cttttcctac ccaaaatggg cagaaattcc aaacattgac ctctatctgg
    16681 accaggtttt gctctatgtc aatcaggtct gcgcccctat ctctcctaat aaagacaagg
    16741 gcctaacagc atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca
    16801 aaaaaaaata ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg
    16861 tattttctat tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag
    16921 accaactcta cgatgctttt gtggactaca tgaaccaagg aattaaccca gctaacccta
    16981 ttatccaaac cagctgccaa accattaaac tctatcatca aactctagac ttaatcgatc
    17041 atactcaaga ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt
    17101 tggagaggag attgctaata gcgtgaccca tgctgtaggt gcagtcatca tgcttatctt
    17161 gctgcctatt tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt
    17221 ttccattttc gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat
    17281 ggcctatggt tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt
    17341 tgccattgcc ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta
    17401 tctgattatt gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc
    17461 taaaaaggtc aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct
    17521 ggctatcatt cctgccatta tcagtcaaac gacacccgtt ttctggagtc tcatggtaac
    17581 tggcggactc tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca
    17641 catgatttgg catctcttta tcctagctgc gtccgcactc caatacatcg ctattgttta
    17701 ttacatgtaa aaaagttgag aagttcaatc tcaacttttt tctttacaca tattgataaa
    17761 gtactggtgc aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt
    17821 tttttcttgg gctgcaacaa tttgattgtt cactgttgct agaatttcta caccctcacc
    17881 aacactactg ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca
    17941 ttctgcttcc gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat
    18001 atccatagtt cctagatgac tctctttctg agaagtgatt tccttagcca gcaaaattaa
    18061 gcccgcacag gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag
    18121 catgttctgg tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa
    18181 acccgacaag tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc
    18241 gacacctaat tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat
    18301 tccgattttc atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat
    18361 accaaccatg gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa
    18421 gttagtcaca gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat
    18481 acctgaaccg acaaagaccc cctctgcccc tagttgcatc attaacgcag catctgctgg
    18541 tgttgcaaca cctccagcag cgaaatttac aactggcaat tttccatgtt catgaacata
    18601 ttggaccaat tctacaggga cttgcaaatc cttggcagca acataaagct cgtcctcacg
    18661 taagttttga atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac
    18721 gatatcccct gtccctggtt ctcctttggt acgaatcatg gaagcacctt cagcgatacg
    18781 acgcaaggct tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt
    18841 gtccacatgg aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat
    18901 ttcaatagcc tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac
    18961 tggaatacta accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac
    19021 tcctccagct gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc
    19081 agcagcttct gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt
    19141 gagcatctgt gccaagtttt tatttagttc ataacgattt tcagtcattt gttttatcct
    19201 catcatttgt attggtagct accttttcat tttaagtcag aacagaatga agcacaagat
    19261 aaaaaaacta taattgtatg gggacaaatt agctaaaaac tatctgacct taacttaagg
    19321 ccagatagct cattcatttt tatttttcag ccgtaagggc agccattgtg atgtagttgt
    19381 atggtttgtt gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca
    19441 catgctcttg gatagcaagt gagaacatgt ggattcccat gctaattgca atatcatgtg
    19501 aaaccatttg ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta
    19561 cttcatggtt gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag
    19621 ttgcgttgta accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa
    19681 gaccgtagat tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac
    19741 aagcattgta ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc
    19801 ttgtatcttt acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg
    19861 ggatagatgt ttcttgtttc ttgtctacaa ggaaggcacc gttgcggaag agttcgatct
    19921 taccatctgc aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt
    19981 caaagctttc tttgtcagta atcaagcgtt caactttacc gtcaccttcg attgctttaa
    20041 cagtttgacc tagagccaag cggatgttgt gatcttccaa gttcttcgcc atcatttgtg
    20101 tgaagtcttt gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca aggacaactt
    20161 cttttccaag acgttcaaag gcttcagcaa gttcaacacc gatgtaacca ccaccaacaa
    20221 cggcgatacg gtcgaggtgt tggctcttgt cagaaagttt attgataact tcttcagcat
    20281 tttggtacaa tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt
    20341 taacaatttc aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca
    20401 atttttcgta tgattctttg tgctcttttc cttcaacttc cgctgtaact actttgttat
    20461 catagtcgat tgaaagaaca ggtgagttca tgtaaacttt agcaccttta gcttccaatt
    20521 tttctttatc agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag
    20581 ccattccaca tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct
    20641 catttccaaa attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga
    20701 ctacaacgat tttactcata aaaatttcct gccttcattc taaatttacc ttactagtat
    20761 accatttacc gttagcgttt acaagggggg tactattatt tcagcatttt ttatcaaaaa
    20821 taattcccca tttatcctgt tttcaagcct catgaatttt atgagcatat ttcttgactt
    20881 tttccaaatt ttgtttgtga aaattctaac tttatggtat tataataaca tgaaaatgaa
    20941 atgtaagggc ttcaatttag ataattattt tatattacaa cttattttct ataagagaaa
    21001 ggagttggta gcttgactct tcgttcacat tctgaacggc taatggggac tactatcact
    21061 atttcattag tggatgagca agccgatatc tttctccaaa aatcctttga cttgctcaaa
    21121 gagcttgaat accgcttcaa cgccaatagt caagaatctg agttgatgga aatcaattat
    21181 caagcaggaa tatcccccgt cacggttcat ccagacctgt ttgagctgat ttcacttggg
    21241 ttagagcata gcctagcgct ctctagccac ctcaatatca gcattggtcc cttaattcaa
    21301 acctggcgta tcggtttttc agatgccaag gtcgcccagc ctcaagaaat tgaatcggtg
    21361 ctgcctctaa tcaatcctca tggtatcgag ttagattctt ctacttctac tgtgttttta
    21421 aaacagaaag gaatgaagat cgatctaggt tgtttagcca aaggatacag tgcggataag
    21481 gttgcccaat ttcttaggaa agagggggtg acttctgcct tgatcaatct gggagggaat
    21541 atcctgacca ttggaaaaaa tcaggcaaga ggggataacc catggcaaat cgggattcaa
    21601 gacccagcca atccgagggg aaatcattta atgaccatcc ctgttgtcaa taaatctgtc
    21661 gtgacttcag gcatttatga acgtcacctg accgtcgatg gacaagatta ccatcacatt
    21721 tttgacagtc aaacaggata tcctgttgaa acggaactag cgagtctaac aatcatctct
    21781 gataaatcag tcgatggcga aatctggacg actcgactct ttggagaaag acctgcttct
    21841 atcctctggc aagtcgaaag tttggagggc atcgaagtca tcctcatcga taaagaaggc
    21901 cacctaagct gttcttcagg aattcctact ctatagagac aactgtttca atggaatttt
    21961 aaaattgtat tatgtaaaaa gagaaaggaa atctcatgtt aaaacttatt gctattgttg
    22021 gaacaaattc aaaacgttct acaaaccgtc aattgcttca atacatgcaa aaacactttg
    22081 ctgacaaagc tgaaattgag cttgttgaaa tcaaggccat tcctgtcttc aacaaaccag
    22141 ctgacaagca agtacctgct gaaatattgg aaattgctgc taaaatcgaa gaggcagatg
    22201 gcgttattat cggtactcct gagtatgatc actctattcc agctgttttg atgagcgctc
    22261 ttgcttggtt gtcttatggt atttacccac ttttgaacaa accaatcatg attacaggtg
    22321 cttcttacgg tacgcttggt tcatctcgtg cccaattgca acttcgtcaa atcttgaatg
    22381 ctcctgaaat caaggcaaat gttcttccag atgaattctt gctctcacac tctcttcaag
    22441 catttaaccc aagtggcgac ttggttgacc ttgatgttat caagaaattg gatgccatct
    22501 ttgatgactt ccgtatcttc gtgaagatta ctgagaaatt gcgcaatgcg caagaattgc
    22561 ttcgcaaaga tgctgaagac tttgactggg aaaatttgta agataggaga ccaaaaagaa
    22621 tgaaatttgt tggacttgtt ggatcaaact acgatcaatc atataaccgt aagctcttgg
    22681 aatttatccg tcgctacttc aaactcaaat ttgaattaga agttcttgaa attgacgaag
    22741 ttccaatgtt taaccaagat gaaaaatggg atgaaagttt ccaattacgt tatttatata
    22801 acaaaattac tcgtgcagat ggtgttatca ttgctactcc tgagcataac cacactatct
    22861 cagcttcact caaatctgta cttgaatggc tttcatacga agttcatcca tttgaaaaca
    22921 agcctgttat gattgtgggt gcatcatact atgaccaagg aacatcacgt gctcaagttc
    22981 accttcgtaa aatccttgat gctccaggtg ttaatgccta cacgcttcca ggtaacgaat
    23041 tccttcttgg taaagctaag gaagcttttg ataacaacgg aaatatcacc aacgaaggca
    23101 ctgttaaatt ccttgaaact tgcttagata actttgttaa atacgtagga gtcgtttcga
    23161 aattgaaaaa accaaaacca atcgaatcag aagacttgga ttgtggaaaa ccaatcgcta
    23221 caaccatcac agaagttgac cctgacgatc cagaatgggt agaaaaagtt gcagcattca
    23281 ctggtgctgt ttctggtgat acctatgtca aattggacca cggtatccta acagttaacc
    23341 aaattgatat gttcttgaaa gctatgccat ttgaattgac atacgctgac gacaacaacc
    23401 aattcctcta ctacaacaac gctcaccaag atccagacac tatgttcgct aaacgtgtac
    23461 cacctcaatc aggtagccgt atgtcgactg ttcatggttc tcttccacca gcacgtatga
    23521 agaacgtaga gtgggttatc ggaacacttc gcaacggaaa ccaagaatac gtccgtacga
    23581 tcgttccagg ttctcctgca ggtgttatca acactcacaa ctaccaagca atgtactatc
    23641 ctgatggatc atacgctggt atcaatgaaa tcgtctttaa cttccaacca tggcttgact
    23701 ggtacctaaa agagactggt caacgtttgg tgggtggtag cggaccattt gctcctgctg
    23761 ccggaggtca tggagacgcc gatgctactt ctggcgcttc tgattcaggg gatgctggag
    23821 gccacggtgg tgacgcagac gctacagccg gtgcaagtta ctaatgatag tttttaggaa
    23881 ccattatggt tcctttttta gcgaaaaaag actagcaaac acaaactgct agtctctttg
    23941 ttatcgtttc atctatcttt gttaaaagaa agaatacata tactcaatga aaatcaaaga
    24001 gcaaactagg aagctagccg caggctgtac ttaagtacgg caaggtgaag ctgacgtggt
    24061 ttgaatttga ttttcgaaga gtattattat gaatttggat catcaagact acggccatgt
    24121 aatccttttt cacgttgtac ttggcgtagt ttttctggtg taacatcgtt tccttcttcg
    24181 tccacaattt tgattccttc aatgtggtga cgaacagcgc ggcgataacc ttcgatgtac
    24241 tcctcacgta gtttggcttg ttccactttt tcttctggtg ttaagccttc tgtttttttc
    24301 tttttagcaa gctcattgat acgagcaatt tttttcggat ccatttcttc tcctctgtaa
    24361 taagataaag tccgtttatc aaattttatt tagtgttaat ttggttaaaa caagcaaact
    24421 tagctgcttt cttggttagt tgtgacaaga ttgccaaacg attttgacgg acagcctgat
    24481 cttcagccat taccatagta ttttcaaaga aagcatcaat gactgggcta agcgcaaaga
    24541 gttgtttcaa ttgctgactt gcaggtcctg ataaaacgag tgtttctact gcttctgcca
    24601 aagctttttc ttggtcattc tcaaatagtg ctgaatcaac cgtagcaacc ccttctgcct
    24661 tctcagccag gttaaaggca cgagaaagtg attcaacaga tggtttaaaa tcttcttcct
    24721 tgcttacttc tacgagagca cttgctgctt ccaacatatc tgccacaaca aagtttgaac
    24781 ctgcaagaac tgcttctttg atatcttttg gagtagagcc catcatctta tcaacacgag
    24841 ccttgataaa gtccataacc tctgctttat tttcataagt caaactgtca aatttcaatg
    24901 cataaaggct atcaatcagc tcatccatag caatgtgcca accaaaggca tccaagatac
    24961 gaaccacacc ttgagttgca cgacgaaggg cataagggtc attagaacct gatggaatca
    25021 atcctactga gaagaaactc aaaatcgtat ccaatttgtc tgcaatggct agaactgcgc
    25081 cgaccttgct ctctggaagt tctccttcag ctgatgtagg catgtagtgt tcacgaatag
    25141 cagctgccac cgctggagtt tcaccagcaa gaagggtgta tttttcaccc ataattcctt
    25201 ggagttcgtc aaattcacca accatacctg tcaacaagtc aaacttgtaa atggctgctg
    25261 cacgagcaag gtcaactgtt tcatccactg acaaactagc tttttctgcc aaaagtacag
    25321 tgatttgacc cgtacgaatc atgtgttcac gaagggaacc aatcttctca tggaaagtga
    25381 cattgtttaa tttttcaaca agatctgaaa tcaccaattt ttggtcttca cgccagaaga
    25441 attctccgtc ttccaagcgg gctaccaaga ctttttcatt tcctttgatg acatttttca
    25501 aacgctctgc gtttccgtta cgaacagaaa tgaagtttgg caagagtttt ccatcttgat
    25561 cacgaacaac aaagtaacgc tggtgttcct tcatagaagt caccaagact tcttctggaa
    25621 cttcaaggta tttagcatca aaacttccca tgaaggcagt tgggtattca accaaattca
    25681 agacttcatt cagcaaatcc gcatcaattt cgatacgtac accatgtttt gcctcaattt
    25741 ccttgatttg gtcaacaatc atttgctcac gttcacatgg atctgcgatt acaaactgct
    25801 tacgaaggtc ttcttcatag ctcaatgctg actgaatttt ggtttctttc cccaagaaac
    25861 gatggccacg gctcacacga cttcccttga tatcaaggaa atccaagtca aactcttgct
    25921 catccaagag aacagttaaa gtgtgaacag ggcggatgta ttcaaagcta tttccagccc
    25981 agtgcatgct gacagggaaa gtcaatgact tcaagacatc tacaacacca ggaacaatgg
    26041 cttcaactga ttggccaact tcttccttag tgacatagac atattcttca cccttaattt
    26101 cacggaattc gatatcttca acagtcaaac ctttcccacg gacaaatcct tgagctgctt
    26161 tggtgaagtt tccatcacta tccaaggcaa ttttctttgc tggtcccttg aaatcttctg
    26221 tcaaatcaga ctgtttgtct gataaaccag tcacacgaac agccaaacga cgtggtgttg
    26281 agaaggtttg aatggcttca aaagacaggc ggttttcctt gaggaaggct gccatttttt
    26341 cgcctagttg tttttcactt ggtgtgacaa cataggctgg taattcttca agaccgagtt
    26401 ctactaataa gttttttgtc atgttttttc ctttacattt tcttcaatct tttttgatat
    26461 gcaccttagg tactggggac gtcgctaact aactctgtga gtctgtatgg aaatccaatg
    26521 ctaaatttat caagatttcc aacaatctca tcaaagtgga tttagctgac tgatttttga
    26581 acctaaggtt tcaaaaatcc ccacataaca gtacggcggt gcatatccct ctagcaagtc
    26641 ttcgacttgc ctctaacgat tttagagtac agtattactc actattctgc gtcttctgct
    26701 aaaagtttaa ctcgtgtttc ttcatccaaa agtgggtaac ctaggcgttt gcgttctgca
    26761 acaaaggttt tggctacaac acgggccaag ttacggatac gagcgatata gcctgcacgc
    26821 tctgttacag atacggcacc acgcgcgtca agcagattaa aggtatgtga acatttgaga
    26881 acatagtcat aggcagggtg taccaagcct tcttctaatg cacgaccagc ttctttttca
    26941 aacttatcaa agttttcaag caacatttct tggtccgaaa tttcaaatga atattttgag
    27001 tgctcatact caggctggat aaagatttct ccgtatttta caccattagc ccactcgata
    27061 tcatagacag agtctacttc ttgaatgtaa gaagccaagc gctccaaacc ataggtaact
    27121 tccgcagtca cagggccagt tgccaatcca ccgacttgtt ggaaataagt gaactgagtg
    27181 atttccattc cgtcaagcca aacttcccaa ccaagaccag ctgaaccagt tgatgggttt
    27241 tcccagttgt cctcaacaaa acgaatatcg tgctccaaag gattgattcc caatttttcc
    27301 aaagactcaa ggtaaagttc ttggatattt gatggagaag gcttcatgac cacctggaat
    27361 tggtggtgtt ggtagagacg gttagggttt tccccataac gaccgtcagc aggacgacgt
    27421 gatggctcta cataagctgc attccatggc tcaggtccga tagcacgaag gaaagtgtaa
    27481 ggactcattg ttcccgcacc tttttcatta tcataagcct gcataagcat acaaccttgg
    27541 tcattccaaa attgttgcaa agtcaaaata atttcttgaa atgttaattt cttagacatt
    27601 gaatactcct taataaaaaa taattacttg cgacacgagt ctgcctcgtc gatcatacga
    27661 ggcaagacga gttagtactg cgtctagctc aggcactcta gtgctgagcg tggagcagcc
    27721 cgactgtaag gaggtttctg ccactgatgc agtgaaatgt taatttctta gacattgaat
    27781 actccttaat aaaaaataat ttcttgcgac acgagtctgc ctcgtcgatt atacgaggca
    27841 agacgagtta gtactgcgtc tagctcaggc actctagtgc tgagcgtgga gcagcccgac
    27901 tgtaaggagg tttctgccac tgatgcagtg aaatgttaat ttctcagaca ttgaatactc
    27961 cttaataaaa aataatttct tgcgacacga gtctgcctcg tcgattatac gaggcaagac
    28021 gagttagtac tgcgtctagc tcaggcactc tagtgctgag cgtgaggcag tccgactgta
    28081 aggaggtttc tgccactgat gcagtgaaat gttaatttct tagacattgt ttactccttt
    28141 ttatataaat tacttgcgac acgagtctgc ctcgtcgatc atacgaggca agacgagtta
    28201 gtactgcgtc tagctcaggc actctagtgc tgagcgtggg gtagcccgac tgtaaggagg
    28261 tctctgccac tgacgcagtg aaatgttaat ttcttagaca ttgtttactc ctttagtttt
    28321 tatatctctt tttcaattaa tttctgctga acaaaccaat ttaacaaggg aagagtttta
    28381 tctgtatttg cccataaatg ataagaatcg aagaccgctt gtacactacc tagattttct
    28441 accaacttat ctattgtcaa aaaactacgc cagtattcgt aaaaaatact tgcataattc
    28501 ttatcataag tcgaagaacc aaaatcattc aatgaatgcc aaccatactt cttctgaaaa
    28561 agttctacaa gagactgatt acaaattttt tccgcttgaa attcctcttc tgtcaagaaa
    28621 tacttgcgac taatatattc aaccatcccc tcttcaaacc agatgtaaga gtcgtaacca
    28681 tcaaaatcat ctaaaaaata ctccgaccaa tgagctaact catgtcctac aatctgtaag
    28741 aggaaatttt cagataaaga atgataatgg ctttctattg cttgagtttg ctgagaacac
    28801 tcgtagtcca tcaactgata caaatacaac tctttccata cagctaaatc aggcgtcata
    28861 accattcgtc tattatttgt ataggctgga acagcgcttt ccctaataat ctgtgtagca
    28921 gcattaaaac ttgaccaaat aacggcttgc ggtaaatcac agactgcaag ttcattcttt
    28981 aaaaaagatt gataatcttt caacttttca gtatttctta ctacaaaatc acgaaatgca
    29041 gctagttgac tatcgtcttt tacaagataa aggttgtcca cggctctcct ttctttcaca
    29101 caatagacta aataagcaaa agaaccgcgt ccgacaatcc taggttgatt taacctaggg
    29161 gcgtcagaaa cgcggttcca ccctaattta ttacttcatt gtttttgaaa atactacaga
    29221 aagcgccagc cctttatttt gccctacttg gctcccacta tcccaagctc gcttgaagaa
    29281 cgccataaga ctttctttcc tgctgactat tatatcaaaa attagaataa tgtacaattc
    29341 tttttatgat tttctagaaa tccacatcat caactcgtgg agcacccgat tgaacagcaa
    29401 tcgtttttaa gttttctctc tcctcatgac tcagttcaat tccaaagcaa tcaagattag
    29461 cttgaatacg agaggttgtg acagattttg gaagtggtaa aaatccttct gccaagctcc
    29521 aggccaaggc tatctgagca atcgattttc cgtgatttgc tgcgatttct tgcacttgct
    29581 tgctatcaaa tagttctccc tgaccaaaag gcccccaagc ttccaataaa attccctttt
    29641 cacgacagta agctacgact tgatcttgat acacacctgg cgccaagcga acttgattga
    29701 ccgcaggaac gatagttgca gtttcaagca aggcatccaa atgatgggga agaaaattgc
    29761 taacgccgat agcacggatt ttcccttctt gatagaggtc ttccatcgct ctccaaactt
    29821 ccgcattgcg agttttccat gcgtcatttt ctctgagcgg ttttgggttc ggccaatgaa
    29881 tcaaatacaa atccaaataa tccaagccca gtttttctat agatttttcc aaagcttgac
    29941 gagtttgctc ataggtttgc tgactattcc aaagcttggt agttacgaac atttcttcac
    30001 gtggaactcc gctatctttg attgcttgac caacactttc ttcattctga taaatcgccg
    30061 ccgtatcaat atgacgataa ccagccttca aggcttctaa cactgcatga taggcttctt
    30121 ctccatcctt agccttaaaa gttccaaatc ccaatactgg aatttctact ccattattta
    30181 attgataggt attcatatgc ttctccttct attttattca tcaattatag cataagacat
    30241 tttttaagta gtactatctt ctgtaaaaaa taagtctgtc aaatatctaa cagacctatt
    30301 ttttcttgct tattcatcac gactatagta cagctcatga gcttttaggc gagtattgag
    30361 catctctggt atggtcacaa aggtataacc ttgatttttc aaatactcaa tgacccttgg
    30421 caaggcattg actgtcggac tgtgaatatc atgcatcaaa acgatagagc cgttagctac
    30481 ttgatgctga atttctgtca aaatagatgc ttcattttta ctcttccagt ccagactatc
    30541 cacatcccac atgataaagc tcaaatccaa gctattgcga atatcatctg taatagcacc
    30601 ataaggtgga cgcatgagtt tagaactaga acccagcact ttagttagca catcctcagt
    30661 atcagtaatc tgctttttag cttcatcaag agagagttgc gagagaatcg gatggctcca
    30721 actatggttt ccaacaacat gaccttcaga ttttatcctc ttcaccaagt cctcattccc
    30781 agaaacattt ttcccaagca caaagaaagt cgctttaata tcatatttag ctagggtctc
    30841 taatacctgc ggggtcgttg ctggatttgg accatcatca aaggttagag cgacaacttt
    30901 ttgatatttc ttatcaaagt aagattggta caaggccgca tctttttcga gtaagtacga
    30961 agattggata acatcaaaga aagcagatac tggcaaggct atctcttcta aattttcaag
    31021 cacaggactt ggataaagga taatctgact atccttgtaa tcaaaattcc atgcagacaa
    31081 gtcttggtca gagaagtttt ttacaatctg ctcactctgg tcttgctcta tttttttatc
    31141 ctctatgaag ggggtcaact cttttatcag ctgttcctta gccttactag catctgaaaa
    31201 cagttggtca agtgtaaaag gttgcccatc ttctgttaag tgaacctttc ctagactggt
    31261 cttttcagtc tcttcaacct taaaagcaac taaatcatag atttgtttgg tcacattacg
    31321 attaacgact ccctttaaac ctgactcttc ttgctctgta tagtagaaaa caagattgtc
    31381 cttactttct agcttgtcct taacatcttg atttatcaac tccctaacag aggaaatcac
    31441 tttctcccct tggagaggat aataggcaat cacttcggct tgcccctgac gaaaatgctc
    31501 cttctgattt ccctcactca attgatcatc tttctctttt ttgagcgatt caatcttttg
    31561 ctcaaaactt ttttgctggt agatcttaaa agcaataaat ccgcctaata ggcaaataga
    31621 aattgctaaa ataccaatca aagccaataa tacatgtctc gttttcccgt gtctgccacg
    31681 tcctagtcta cttttattca tatttatatc ataacaaatt tgagcaataa tttcaatgat
    31741 acaatttggc aatccacaat tcctctagca agccaaagca tcttacatca actccaacat
    31801 ccgtatctgt agagattatg tagttttcta tctatggaat ttctagtaga ttgaagctag
    31861 aatagtagac ctatacttct aaaacattgt tagaaatcga tttgattgtc ctgatcgatt
    31921 tgtcctgttc ttatttcatt ttactataaa actatgagaa gctaggtcat ctgagaagta
    31981 attactatcg acaattgcga tagcgtatac tggtgcgatg tgtttgtagc tctggtgggt
    32041 atcaccttca cgttggcgaa ttttttctag gttttgatta acctgactgc acagataagc
    32101 ccataggcga ttgatgaaaa aattctgatg atgaacctga atttcgatga taacttggat
    32161 tccattatct aactccgcca agacgtctat actagtatag aaatcctgcg ctgagtaggg
    32221 catggaaggc aagacgtgaa tgttacttcc ctccaaaatc gtcacatttt ttgctggcaa
    32281 gtccagcata tcgcggataa attgacaagt gatttctgga ttgctgaata tttttttagc
    32341 aaccaaatca ttagttgggc tgatgcccgg atgtctgaga atcctccttc tattatagca
    32401 catttttaaa tctaggtttg ctagattcta tgcttctatc tatttattcg gaaaagaaag
    32461 ctaatagctt atttgaaatt ttctcctctc tgttggatag tcccgtctat ctatgttata
    32521 atgaaagaag gatttaaaat cggaaaagga gtatttatgc taaaattagg tgtcatcgga
    32581 actggcgcta tcagccatca tttcatagaa gcagcccata ccagtggaga ataccaactg
    32641 gtcgcaatct attctagaaa actagaaact gcagcaacct ttgcttctcg ctatcagaat
    32701 atccaactat tcgatcaatt agaagtcttc ttcaaatctt cttttgattt agtctatatc
    32761 gccagtccaa actccttaca ttttgctcag gcaaaagctg ccttgtctgc tggtaaacat
    32821 gttattctcg aaaagccagc tgtcactcaa ccacaagaat ggtttgattt gattcaaaca
    32881 gctgaaaaaa ataactgttt tatctttgaa gcagctcgta attaccacga gaaagccttt
    32941 actactatca aaaacttttt agcagataag caagtgttag gagcagattt caactatgcc
    33001 aaatactctt ccaagatgcc tgacttgttg actggacaaa caccaaatgt cttttcaaac
    33061 cgttttgctg gtggagccct catggatttg gggatttatc ccctctatgc tgctgttcgt
    33121 ctttttggaa aagccaatga cgcgacttac catgctcaac agcttgacaa tagcattgac
    33181 ctaaatggag atggtatcct cttctaccca gactatcaag ttcacatcaa ggctggaaaa
    33241 aacatcactt ccaatcttcc ttgcgagatt tatacaacag atggaacctt gactctcaac
    33301 acgattgagc atattcgctc agctattttt accgaccacc aaggaaatca agtccagctt
    33361 cctatccaac aggctcctca tacgatgact gaggaagtcg ctgcatttgc acacatgatc
    33421 cagcaaccag atctgaatct ctaccaaact tggctgtatg atgcaggctc tgttcatgag
    33481 ctactatata ccatgcgcca gactgctggc attagatttg aggcagaaaa atgaaaacca
    33541 aactaccgac tgaatggcaa gaactgagtg accaactcgg tttccaagaa ttcaccccca
    33601 ttcaaactca actatttgag ccccttcttg ctggagaaaa cctcctagga gtgagccaaa
    33661 ccggaactgg taagacccta gcgtatctcc taccaagtct tctcagacta caaaagaaaa
    33721 aagcccaaca actcttgatt ctagcaccaa acacggaact tgctggacag atttttgatg
    33781 tatgtaaaac gtgggcagaa gctatcggat tgactgctca actcttcctt tcaggttcaa
    33841 gtcagaaacg acaaattgaa cgccttaaaa aggggccaga aattctgatt ggaactcctg
    33901 gccgtatctt tgaactcatt aaacttaaaa aaatcaagat gatgaatgtg gaaaccatca
    33961 tcctggatga atttgaccaa ttactagatg attctcagat tcactttgta gaaaaaatca
    34021 ctcactacgc acctcgtgac caccaactgg tctacatgag tgccacgacc aagtttgatc
    34081 aagaaaagat tgtgccgaat acacgcacta ttgatctctc tgatcaaaaa ttggacaata
    34141 tccagcattt ctacatgcag gtagaccaac gtcatcgagt ggatatacta cgaaaactgg
    34201 ctcatgtaga ggatttccgt ggtctagtct tcttcaatag cctgtcagac cttgggaatg
    34261 ccgaggaaaa actacagtat cgtgatatat tggctgtttc cctcgctagt gatgttaatg
    34321 ttaaatttag aaaagtcatc ttagaaaagt ttaaagataa ccaactaacc ctgcttcttg
    34381 caactgacct tttggctcgt ggaattgata tcgatagcct agaatgtgtc gtaaactttg
    34441 atattcctag agatagcgaa acctacacac accgtgctgg ccgtactggc cgtatgggca
    34501 aggaaggata tgttattact ctcgtcactc atccagaaga aatcaaaaaa cttaaaaagt
    34561 ttgcaagtat ccgagaaatt gtcctaaaaa accaagaact ctatatcaaa taaagcccca
    34621 ttgctctata cacatccagt gtatggagct tttttataag caattcaatt ttatactcat
    34681 ctgctttcaa aaagcattct attccatctc cgattaacga tggactttat cacctccttc
    34741 tccagtcctt gtatgacatc ttggagttga ttcatgacat cttccaaagt tcgaaaggct
    34801 ttattcttaa atccacgttt acgaatctct ttccacactt gttcaatggg ttcatctctg
    34861 gtgtgtatgg aggaataaag gtaaaatcaa tattagtcgg aatctttaag gtacttgatt
    34921 tatgccatat agcattgtcc ataacgagta aaaaataatc atctggataa gcttgtgaaa
    34981 gctcttctaa aaagacgttc atccactcag tattacatcc accagctatt aagaaaaatg
    35041 attcgcctgt atggggcatc aacagctcca taacaatagc gaaattctcg tatatagtga
    35101 ctatggacat gtggacctac tcctattgga gaccaacaag atcccagttt actgattcta
    35161 ccgaaaccag cctcatcttg gtacatcaag cgaaccttat gaaaaggtct actggtttta
    35221 aagcgctttc ttgtcttctt gaattgagat tttattttta gacgcgacaa tggtttgagc
    35281 gtctgctttc ttaggatgtt ctggacgtgg cataatattt cgccaaccat ggtgcttcaa
    35341 cagttgatag aaggcatcac gtgtgtagga acgacctaac tcctttttat aagcctgaaa
    35401 taaggcatca attgtaacaa attctcctgc ctctgtagcc ttcaaatggc gggcaagaaa
    35461 ggctttctct tcctcaactg tcatatatgc atggttacga ccaccacgtg tttcttgaag
    35521 gagagagtcg agtccgaact cctcatattt ttttacattt cgccaaatcg ttgtttgatt
    35581 acagtctaaa agatctataa tctctttata agatttgccc atcagacgaa atagaacgat
    35641 ttgaaggcgt ttatgatatt tagctgtacg aaagtctttt aaaagtgttt tgatggtttg
    35701 gatttcttct tcagttgatt tcatattact attgtataat gctttttgat ttgagtctag
    35761 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
    35821 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgagcagt ctgcagctaa
    35881 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagacaatc aa
//
LOCUS       DAVMAF010000013        35421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMAF010000013 DAVMAF010000000
VERSION     DAVMAF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.013"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            176..2158
                     /locus_tag="VYO97_000643"
     CDS             176..2158
                     /locus_tag="VYO97_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753483.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAANKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2458..7761
                     /locus_tag="VYO97_000644"
     CDS             2458..7761
                     /locus_tag="VYO97_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1753484.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSIWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPLYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7928..10087)
                     /locus_tag="VYO97_000645"
     CDS             complement(7928..10087)
                     /locus_tag="VYO97_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1753485.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10084..10680)
                     /gene="recU"
                     /locus_tag="VYO97_000646"
     CDS             complement(10084..10680)
                     /gene="recU"
                     /locus_tag="VYO97_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1753486.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10746..11273
                     /locus_tag="VYO97_000647"
     CDS             10746..11273
                     /locus_tag="VYO97_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1753487.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11343..11672
                     /gene="gpsB"
                     /locus_tag="VYO97_000648"
     CDS             11343..11672
                     /gene="gpsB"
                     /locus_tag="VYO97_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1753488.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11693..12073
                     /gene="rnpB"
                     /locus_tag="VYO97_000649"
     ncRNA           11693..12073
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYO97_000649"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12158..13315
                     /locus_tag="VYO97_000650"
     CDS             12158..13315
                     /locus_tag="VYO97_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1753489.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13328..14722
                     /gene="mapZ"
                     /locus_tag="VYO97_000651"
     CDS             13328..14722
                     /gene="mapZ"
                     /locus_tag="VYO97_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1753490.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14798..16222
                     /gene="gndA"
                     /locus_tag="VYO97_000652"
     CDS             14798..16222
                     /gene="gndA"
                     /locus_tag="VYO97_000652"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1753491.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLNKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16234..16923
                     /locus_tag="VYO97_000653"
     CDS             16234..16923
                     /locus_tag="VYO97_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753492.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17021..17986
                     /gene="cbpC"
                     /locus_tag="VYO97_000654"
     CDS             17021..17986
                     /gene="cbpC"
                     /locus_tag="VYO97_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1753493.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            18005..19003
                     /gene="cbpJ"
                     /locus_tag="VYO97_000655"
     CDS             18005..19003
                     /gene="cbpJ"
                     /locus_tag="VYO97_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1753494.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            19125..20003
                     /gene="mvk"
                     /locus_tag="VYO97_000656"
     CDS             19125..20003
                     /gene="mvk"
                     /locus_tag="VYO97_000656"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1753495.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSYGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            19985..20938
                     /gene="mvaD"
                     /locus_tag="VYO97_000657"
     CDS             19985..20938
                     /gene="mvaD"
                     /locus_tag="VYO97_000657"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1753496.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            20925..21932
                     /locus_tag="VYO97_000658"
     CDS             20925..21932
                     /locus_tag="VYO97_000658"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1753497.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVFLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            21916..22926
                     /locus_tag="VYO97_000659"
     CDS             21916..22926
                     /locus_tag="VYO97_000659"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1753498.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIVDLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            23003..23701
                     /locus_tag="VYO97_000660"
     CDS             23003..23701
                     /locus_tag="VYO97_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1753499.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            23698..24693
                     /locus_tag="VYO97_000661"
     CDS             23698..24693
                     /locus_tag="VYO97_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1753500.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            24707..25339
                     /locus_tag="VYO97_000662"
     CDS             24707..25339
                     /locus_tag="VYO97_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753501.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQADVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            25340..25581
                     /locus_tag="VYO97_000663"
                     /pseudo
     CDS             25340..25581
                     /locus_tag="VYO97_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            25506..25817
                     /locus_tag="VYO97_000664"
     CDS             25506..25817
                     /locus_tag="VYO97_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1753502.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLQLA
                     S"
     gene            25842..26108
                     /locus_tag="VYO97_000665"
                     /pseudo
     CDS             25842..26108
                     /locus_tag="VYO97_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            26258..26497
                     /locus_tag="VYO97_000666"
     CDS             26258..26497
                     /locus_tag="VYO97_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753503.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            26578..27399
                     /gene="cbpG"
                     /locus_tag="VYO97_000667"
     CDS             26578..27399
                     /gene="cbpG"
                     /locus_tag="VYO97_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1753504.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKNAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GIIVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            27418..28440
                     /gene="cbpF"
                     /locus_tag="VYO97_000668"
                     /pseudo
     CDS             27418..28440
                     /gene="cbpF"
                     /locus_tag="VYO97_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(28568..29703)
                     /locus_tag="VYO97_000669"
                     /pseudo
     CDS             complement(28568..29703)
                     /locus_tag="VYO97_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            29912..31681
                     /locus_tag="VYO97_000670"
     CDS             29912..31681
                     /locus_tag="VYO97_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1753505.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPIANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFFVA
                     ALILHADKSTEDSLEAAQEATQAAKAQSKGQLVSTSVDAVISTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            31705..33660
                     /locus_tag="VYO97_000671"
     CDS             31705..33660
                     /locus_tag="VYO97_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1753506.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            33662..34099
                     /locus_tag="VYO97_000672"
     CDS             33662..34099
                     /locus_tag="VYO97_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753507.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            34161..35297
                     /locus_tag="VYO97_000673"
     CDS             34161..35297
                     /locus_tag="VYO97_000673"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1753508.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT        10812 a         6282 c         7946 g        10381 t
ORIGIN      
        1 agaaatagta ttaaaaattc cttgactatg tgatatagtt gagggatttt taaatgatat
       61 tcatattttt tgcaaagatg ttgtttgaaa aataattttc aaaaattctg aaaattctgt
      121 tgacaacttt ctgaaaagag tctataatgg agagaaagtt ttaaaggaga aaaggatgaa
      181 aagttcaaaa ctacttgccc ttgcgggcgt gacattattg gcggcgacta ctttagctgc
      241 atgctctgga tcaggttcaa gcactaaagg tgagaagaca ttctcataca tttatgagac
      301 agaccctgat aacctcaact atttgacaac tgctaaggct gcgacagcaa atattaccag
      361 taacgtggtt gatggtttgc tagaaaatga tcgctacggg aactttgtgc cgtctatggc
      421 tgaggattgg tctgtatcca aggatggatt gacttacact tatactatcc gtaaggatgc
      481 aaaatggtat acttctgaag gtgaagaata cgcggcagtc aaagctcaag actttgtaac
      541 aggactaaaa tatgctgcta ataaaaaatc agatgctctt taccttgttc aagaatcaat
      601 caaagggttg gatgcctatg taaaagggga aatcaaagat ttctcacaag taggaattaa
      661 ggctttggat gatcagacag ttcagtacac tttgaacaaa ccagaaagct tctggaattc
      721 taagacaacc atgggtgtgc ttgcgccagt taatgaagag tttttgaact caaaagggga
      781 tgattttgcc aaagcgacgg atccaagtag tctcttgtat aacggtcctt atttgttgaa
      841 atccattgtg acaaaatcct ctgttgaatt tgcgaaaaat ccgaactact gggataagga
      901 caatgtgcat gttgacaaag ttaaattgtc attctgggat ggtcaagata ccagcaaacc
      961 tgcagaaaac tttaaagatg gtagccttac agcagctcgt ctctatccaa caagtgcaag
     1021 tttcgcagag cttgagaaga gtatgaagga caatattgtc tatactcaac aagactctat
     1081 tacgtatcta gttggtacaa atattgaccg tcagtcctat aaatatacat ctaagaccag
     1141 cgacgaacaa aaggcatcga ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat
     1201 tgcctttggt tttgatcgta cagcctatgc ctctcagttg aatggacaaa ctggagcaag
     1261 caaaatctta cgtaatatct ttgttccacc aacatttgtt caagcagatg gtaaaaactt
     1321 tggcgatatg gtcaaagaga aattggtcac ttatggggat gaatggaagg atgttaatct
     1381 tgcagattct caggatggtc tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc
     1441 taaatcagcc ttacaagcag aaggtgtgac attcccaatt catttggata tgccagttga
     1501 ccaaacagca actacaaaag ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac
     1561 tttaggagct gataatgtca ttattgatat tcaacaacta caaaaagacg aagtaaacaa
     1621 tattacatat tttgctgaaa atgctgctgg cgaagactgg gatttatcag ataatgtcgg
     1681 ttggggtcca gactttgccg atccatcaac ctaccttgat attatcaaac cttctgtagg
     1741 agaaagtact aaaacatatt tagggtttga ctcaggggaa gataatgtag ctgctaaaaa
     1801 agtaggtcta tatgactacg aaaaattggt tactgaggct ggtgatgagg ctacagatgt
     1861 tgctaaacgc tatgataaat acgctgcagc ccaagcttgg ttgacagata gtgctttgat
     1921 tattccaact acatctcgta cagggcgtcc aatcttgtct aagatggtac catttacaat
     1981 accatttgca ttgtcaggaa ataaaggtac aagtgaacca gtcttgtata aatacttgga
     2041 acttcaagac aaggcagtca ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa
     2101 agaaaaagaa gagtctaata aaaaggctca agaagatctc gcaaaacatg tgaaataact
     2161 gttgcaaaat ataagaaagg atttagtatt tctcttgaat gctgaatcct tttttacatt
     2221 tgtaaagaaa gattctaaat gtactgaccc ccaaaagttg gagcctcttt ttgtcagaat
     2281 agagaaaatt tttgttaatt ttacttgttt cctattgctt tctcagttat tatttgttat
     2341 attaaaagta taattatttt tatttatcag ggttaagcat tgcactttca gaggaaggag
     2401 tattttttaa aaaggaaatg taaacgcttg ctaaaaaatg aaaggattta gaagtttatg
     2461 aataaaggat tatttgaaaa acgttgtaaa tatagtattc ggaaattttc attaggtgtt
     2521 gcttctgtta tgattggagc tgcattcttt gggacaagtc cggttcttgc agatagcgtg
     2581 cagtctggtt ccacggcgaa cttaccagct gatttagcta ctgctcttgc aacagcaaaa
     2641 gagaatgatg ggcgtgattt tgaagcgcct aaggtgggag aagaccaagg ttctccagaa
     2701 gttacagatg gacctaagac agaagaagaa ctattagcac ttgaaaaaga aaaaccggct
     2761 gaagaaaaac caaaagagga taaacctgca gctgctaaac ctgaaacacc taagacggta
     2821 actcctgaat ggcaaacggt agagaaaaaa gaacaacagg gaacagtcac tatccgagaa
     2881 gaaaaaggtg tccgctacaa ccaactatcc tcaactgctc aaaatgataa cgcaggcaaa
     2941 ccagccctgt ttgaaaagaa gggcttgacc gttgatgcca atggaaatgc aactgttgat
     3001 ttaaccttca aagaagattc tgaaaagggc aaatcacgct ttggtgtctt cctgaaattt
     3061 aaagatacca ataataatgt ttttgtcggt tatgacaagg atggctggtt ctgggaatac
     3121 aaaactccag gtaatagcat atggtataaa ggcaaccgtg ttgctgctcc tgaaacagga
     3181 tcaataaacc gtctttctat cactctcaag tcagacggtc agctaaatgc cagcaataac
     3241 gatgtcaatc tctttgacac agtgactcta ccagctgcgg tcaatgacca tcttaaaaat
     3301 gagaagaaga ttcttctcaa ggcgggctct tatgacgatg agcgaacagt tgttagcgtt
     3361 aaaacggata accaagaggg ggtaaaaaca gaggataccc ctgctgaaaa agaaacaggt
     3421 cctgaagttg atgatagcaa ggtgacttat gacacgattc agtctaaggt cctcaaagca
     3481 gtgattgacc aagccttccc tcgtgtcaag gaatacagct tgaacggaca tactttgcca
     3541 ggacaggtgc aacagttcaa ccaagtcttt atcaataacc accgaatcac ccctgaagtc
     3601 acttataaga aaatcaatga gacaacagca gagtacttga tgaagcttcg cgatgatgct
     3661 cacttaatca atgcggaaat gacagtacgc ttgcaagttg tggacaatca attgcacttt
     3721 gatgtgacca agattgtcaa ccacaatcaa gtcactccag gtcaaaagat tgatgacgaa
     3781 agaaaactac tttcttctat tagtttcctc ggcaatgctt tagtctctgt ttctagtgat
     3841 caaactggtg ctaagtttga tggggcaacc atgtcaaaca atacgcatgt cagcggagat
     3901 gatcatatcg atgtaaccaa tccaatgaag gatttggcta agggttacat gtatggattt
     3961 gtttctacag ataagcttgc tgctggtgtt tggagtaact ctcaaaacag ctatggtggt
     4021 ggttcgaatg actggactcg tttgacagct tataaagaaa cagtcggaaa tgccaactat
     4081 gtaggaatcc acagctctga atggcaatgg gaaaaagctt ataagggcat tgttttccca
     4141 gaatacacga aggaacttcc aagtgctaag gttgttatca ctgaagatgc caatgcagac
     4201 aagaacgttg attggcaaga tggtgccatt gcttatcgta gcattatgaa caatcctcaa
     4261 ggttgggaaa aagttaagga tatcacagct taccgtatcg cgatgaactt tggttctcaa
     4321 gcacaaaacc cattccttat gaccttggat ggtatcaaga aaatcaatct ccatacagat
     4381 ggtcttgggc aaggtgttct ccttaaagga tatggtagcg aaggccatga ctctggtcac
     4441 ttgaactatg ctgatattgg taagcgtatc ggtggtgtcg aagacttcaa gaccctaatt
     4501 gagaaggcta agaaatatgg agctcatcta ggtatccacg ttaacgcttc agaaacttat
     4561 cctgagtcta aatacttcaa tgaaaaaatt ctccgtaaga atccagatgg aagctatagc
     4621 tatggttgga actggctaga tcaaggtatc aacattgatg ctgcctatga cctagctcat
     4681 ggtcgtttgg cacgttggga agatttgaag aaaaaacttg gtgacggtct cgactttatc
     4741 tatgtggacg tttggggtaa tggtcaatca ggtgataacg gtgcctgggc tacccacgtt
     4801 cttgctaaag aaattaacaa acaaggctgg cgctttgcga tcgagtgggg ccatggtggt
     4861 gagtacgact ctaccttcca tcactgggca gctgacttga cctacggtgg ctacaccaat
     4921 aaaggtatca acagtgccat cacccgcttt atccgtaacc accaaaaaga tgcttgggtt
     4981 ggggactaca gaagttatgg tggtgcagcc aactatccac tgctaggtgg ctacagcatg
     5041 aaagactttg aaggctggca aggaagaagt gactacaatg gctatgtaac taacctcttt
     5101 gcccatgacg tcatgactaa gtacttccaa cacttcactg taagtaaatg ggaaaatggt
     5161 acaccggtga ctatgaccga taacggtagc acctataaat ggactccaga aatgcgagtg
     5221 gaattggtag atgctgacaa taataaagta gttgtaactc gtaagtcaaa tgatgtcaat
     5281 agcccactat accgtgaacg tacagtaacg ctcaacggac gtgtcatcca agatggttca
     5341 gcttacttga ctccttggaa ctgggatgca aatggtaaga aactttctac tgataaggaa
     5401 aagatgtact acttcaatac gcaggccggt gcaacaactt ggacccttcc aagcgattgg
     5461 gcaaagagca aggtttacct ttacaagcta actgaccaag gtaagacaga agagcaagaa
     5521 ctaactgtaa aagatggtaa aattacccta gatcttctag caaatcaacc atacgttctc
     5581 tatcgttcga aacaaaccaa tcctgaaatg tcatggagtg aaggcatgca catctatgac
     5641 caaggattta atagcggtac cttgaaacat tggaccattt caggcgatgc ttctaaggca
     5701 gaaattgtca agtctcaagg ggcaaacgat atgcttcgta ttcaaggaaa caaagaaaaa
     5761 gttagtctca cccagaaatt aactggcttg aaaccaaata ccaagtatgc cgtttatgtt
     5821 ggtgtagata accgtagtaa tgccaaggca agtatcactg tgaatactgg tgaaaaagaa
     5881 gtgactactt ataccaataa gtctctcgcg ctcaactatg ttaaggccta cgcccacaat
     5941 acacgtcgtg acaatgctac agttaacgat acaagttact tccaaaacat gtacgccttc
     6001 tttacaacag gatcggacgt ctcaaatgtt actctgacat tgagtcgtga agctggtgat
     6061 caagcaactt actttgatga aattcgtacc tttgaaaaca attcaagcat gtatggagac
     6121 aagcatgata caggtcaagg cactttcaag caagactttg aaaatgttgc tcagggtatc
     6181 ttcccatttg tagtgggtgg tgtcgaaggt gtcgaagaca accgtactca cttgtctgaa
     6241 aaacacgatc catatacaca acgtggttgg aatggtaaga aagtcgatga tgttatcgaa
     6301 ggaaattggt cactcaagac aaatggacta gtgagccgtc gtaacttggt ttaccaaact
     6361 attccgcaaa acttccgttt tgaagcaggt aagacctacc gtgtaacctt tgaatacgaa
     6421 gcaggatcag acaataccta tgcttttgta gtcggtaagg gagaattcca atcaggtcgt
     6481 cgtggtactc aagcaagcaa cttggaaatg catgaattgc caaatacttg gacagattct
     6541 aagaaagcca agaaggtaac cttccttgtg acaggtgcag aaacagggga tacttgggta
     6601 ggtatctact caactggcaa tgcaagtaat actcgtagtg attctggtgg aaatgccaac
     6661 ttccgtggtt ataacgactt catgatggat aatcttcaaa tcgaagaaat taccctaaca
     6721 ggtaagatgt tgacagaaaa tgctctgaag aactacttgc caacggttgc catgactaac
     6781 tacaccaaag agtctatgga tgctttgaaa gaggcggtct ttaacctcag tcaggccgat
     6841 gatgatatca gtgtggaaga agcgcgtgca gagattgcca agattgaagc cttgaagaat
     6901 gctttggttc agaagaaaac ggctttggta gcagaagact ttgcaagtct tacagctcct
     6961 gctcaggctc aagaaggtct tgcaaatgcc tttgatggaa acttatctag tttatggcat
     7021 acatcatggg gcggaggaga tgtaggcaag cctgcaacca tggtcttgaa agaagcaaca
     7081 gaaatcactg gacttcgtta tgttccacgt ggatcaggtt caaatggtaa cttgcgtgat
     7141 gtgaaacttg ttgtgacaga tgagtctggc aaggagcata cctttactgc aactgattgg
     7201 ccagataaca ataagccaaa agacattgat tttggtaaga caattaaggc taagaaaatt
     7261 gtccttacag gtactaagac ttacggagat ggtggcgata aataccaatc tgcagcggaa
     7321 cttatcttta ctcgtccaca ggtagcagaa acacctcttg acttgtcagg ctatgaagca
     7381 gctttggcta aggctcagaa attaacagac aaagacaatc aagaggaagt agctagcgtt
     7441 caggcaagca tgaaatatgc gacggataac catctcttga cggaaagaat ggtggaatac
     7501 tttgcagatt atctcaacca attaaaagat tctgctacga aaccagatgc tccaactgta
     7561 gagaaacctg agtttaaact tagctcttta gtttccaatc aaggtaagac gccagattat
     7621 aagcaagaaa tagatagacc agaaacacct gaacaaatct tgccagcaac aggtgagagt
     7681 caatctgaca cagccctctt cctagcaagt gttagtctag ccctatctgc tctctttgta
     7741 gtaaaaacga agaaagacta gtatttagta aaacctctta acaagattac ggaagcagtc
     7801 tctatctttt ccaatgaggt ttatagtaca gaaaaagcct gagaagatgt cttctcaggc
     7861 ttttgttaag cacataaata caatagtgct atgacaaaat cacccagaaa aatctgggtg
     7921 ataaatgtta tggttgtgct ggttgaggat tctgattttg ttgatcaggg gttgtatttg
     7981 attgttgcgt attattgtta ggattggtag tcgtactatt atttgtgctt ggagtggttg
     8041 agctagactg tgaagttgaa ctatctgatg atgagcttga actttcagtt gatgggggtt
     8101 gttgtggagc aggtgagttc cacgtagaac gagcaccatt tttaaatacg aattctccat
     8161 ttctgtagag cccctctggt atattccaat cctctggatt gcttccttca gacagatagg
     8221 tcatcataga gcggtaaact ttggcagcga ccgtaaggcc attgcctaca agtggtgtca
     8281 gacggttaga atagcctgtc catacagcca ttgaatattt acgcgtatag ccagcaaata
     8341 gttcatcagg tgctacaaat tgagaggtct tgatgtggtt ttcaatttcc tcgtctgtat
     8401 agttagaggt tcctgtttta ccagcctgag ggagccaagc aagataggca tttcgtccag
     8461 ttccataact caagactgtt ttcatcatgt cggtcatcat ataggctgtc gtttccttca
     8521 tggcacgagt tccgacatta gagaactctt tttcactccc atcactaaag acgactttat
     8581 ggatatacat tggtttatag taagttccac catttgcaaa ggcagcgtaa gcagcagcca
     8641 tcttttcact acttgctcca tattttttgt ctgattcggt tgtgttactt gaaatggcat
     8701 ttgagtagtg aatacttggg tagtcgattc cgagaccatt taggaaagtc ttggcgcggt
     8761 tgagtccgac cttgtttaga gtttccacgg ctgggacgtt tcgcgattgt tgcagggcgt
     8821 attgcaaggt gatgttgcca aagtagcccc tatcccagtt ataaacaggg gtatttgtcc
     8881 cagggtagtt atagggctca tcgtgaacga tagtagcagt tgaatcgtag ataccgtact
     8941 ccaaggcagg agcatagtct gtgatcggtt tcatagttga tccccagtcg cggtttgttt
     9001 ctactgcttg gttaattccg aaggaaacat tacttgactg atggcgtgct cctagctggg
     9061 caatgacttt accgttagaa acatcaacaa tggtagaagc gacttgcaat tcatcgtctg
     9121 gataggcaac gtattcgtct gtattgtaaa tatcccacag atgtttttga gcttcttggt
     9181 ctacatttgt gtagacatcc atcccagttg tgagcaggtt atagcctgtt tcttcttcaa
     9241 cttgattgat gacttccttg aggtaattat ccatgtaagc agggtaatta cttgctgatt
     9301 tgagactttg tagtccatca gtaattggtg tattgactgc tttctcatac tgttcagcag
     9361 agatgtagcc ttgatttttc atttcagata agaccaagtt tcggcggtct tgggctgctt
     9421 ctggatgtga ataggggtca tattggtttg gtgcctgagg cattccagcc agcaaggcta
     9481 actgaggtaa acttaaatta ttgaggtctt taccatagta gttttgagct gctgtctgca
     9541 ttccatagtt cccattagac atgtagacct tatttatata gtaggtcaag atttcttgct
     9601 tggttgcttt ttgttctaac tgaatcgcta accaagcttc ctgagcctta cgagaaatag
     9661 tctggtcgga agtcgaagtt gaaaagtaag tcaacttaat caactgttga gtgagagttg
     9721 atccaccttg gagggaattg ctttgcagat tgcgcaagaa agctcccagg atacggatgg
     9781 tatcaatccc cctgtggtcg aagaagcgat ggtcttcgat agaaacgatt gctttaacca
     9841 aatctgtggg aatatcatta gcttgggcat tgacgcggcg ttcagaaccc aagtcagcaa
     9901 tgagttgatt tttattgtcg tagattttac tagaagttgt tgcaactagt ttactctcgg
     9961 ataggctagg agccttgcta acgtagtaga aaaaaactcc tccgcctaag acaatggctg
    10021 cgataaccaa gcttaagaag ctaatgctca gatacttgat taggcgcaga atcgttggtt
    10081 tgttcatctt gttttaccac ctaataaatg ttctttgata acattgagat aaggaatttg
    10141 agggaaggca ccagccttga tttcatatcc atattctcga atatattcaa gtggcattga
    10201 tttttgtccc ttatcttgat gatagaagcg aatcaaatcg aatgccggca ataagtaggt
    10261 ttcttgctga gaagaaaagt gaagaaggac aaagcagatt ccttgttggg caaggacttg
    10321 ttccatatgc tgaatctgat gtggatgaaa atttttcatc ggaatcgcac gtttttgttt
    10381 tgtttccttg acttcaaagt cgatgtaata tccattataa acgccagaat agtccgtcgt
    10441 tgaagcttgt cgaaaatagg cttcaacaat cttggcacga cttcgttgtg gatagtccac
    10501 ttgtacgatt tgaataggag ttggtttctt atgtataaca gccaagccct gagacaaata
    10561 gtagtcgttg gtagcattga tcatcttttc aaaagacatt cctcgatttg cgaaattttt
    10621 gggttgagaa agagatgttt gtcttttttg tgatgaaact ttatgtggat agttgaccat
    10681 aattctcctt attggtacaa taacatcact ttattatacc ataatttgca cagaaagggt
    10741 taaaaatggc tacagctttg gttttaggct attctgcttt tgatttgggt ttattttctg
    10801 ataaggatcc tcgtcttaaa ttgattaaaa aagcgatacg taaagattta gaggctatgg
    10861 cagcagatgg ggtgtcttgg cttgtgttta cagggacttt gggctttgaa tattgggttt
    10921 tagaggttgc tcaggagatg aaaaccgaat acggtttcca gttggccacc atttttgctt
    10981 ttgaaaccca tggggaaaat tggaatgaag gcaatcagat gaaactgagt cgctttaagc
    11041 aggtagactt tgtcaaatat gcctatcctc gctatgaaca caaggggcag ttaagagatt
    11101 accagcagtt tttgctggaa aacacgacta gttcctatct tttttatgat gaagaaaatg
    11161 aaacaaaact agcttatttt taccaaaaga tgaaaaatca agaggactat tttataaaga
    11221 gattaacatt tgatcaatta aatgaacttg ctgaaaattt ttccgaaaat tgaagctttg
    11281 accttgattt ttgtttgcct ttttttatat aataatacta gcaagcgaga atggagagag
    11341 acatggcaag tattattttt tcagcgaaag atatttttga acaggagttt ggacgtgaag
    11401 tccgtggcta taataaagta gaagttgacg agtttttaga cgatgtcatc aaggactatg
    11461 aaacctatgc tgccttggtc aagtcacttc gtcaggaaat tgcggatttg aaggaagaat
    11521 taactcgtaa accgaaacct tcaccagttc aagcagaacc ccttgaagcg gcaattacaa
    11581 gttctatgac gaattttgat attttgaaac gcctgaatag attggaaaaa gaagtttttg
    11641 gtaaacaaat tttagataac tcagattttt aagtagttat ttgagatgtg caatttttgg
    11701 ataatcgcgt gaggagaatt gcttctcatg aggaaagtcc atgctagcac aggctgtgat
    11761 gcctgtagtg tttgtgctag gcgaaaccat aagcctaggg acgagaaatc gttacggcag
    11821 ttgaaatggc taagtccttg gataggccag agtaggcttg aaagtgccac agtgacgaag
    11881 tctttctgga aacagagaga gtggaacgcg gtaaacccct caagctagca acccaaattt
    11941 tggtcggggc atggagtacg cggaaacgaa cgtagtattc tgactgctat cagctagagc
    12001 tgttagtggt agacagatga ttatcgaagg aagtggtcct agtcacttct ggaacaaaac
    12061 atggcttata gaaaattgca tataggttgg ggctgagaaa ttttctcaac ctcatttttt
    12121 aaagtggaca tatagaaagg tcttgcaaga ctgtaacatg aaaaaagaat ttaatttaat
    12181 tgcaactgcg gcagcagggc ttgaggctgt cgttggtcgt gaagtgcgag agttgggcta
    12241 cgattgtcag attgaaaatg gacgtgttcg ttttcaagga gacgtgagag ctattatcga
    12301 aaccaacctt tggctgcggg cagcagatcg tatcaaaatt atcgtaggaa cgttccctgc
    12361 taagactttt gaagagctat ttcagggagt tttcgcttta gattgggaaa attatttacc
    12421 acttggagct cggttcccga tttcaaaagc taaatgtgtt aagtccaaac ttcacaatga
    12481 gcccagtgtt caggctattt ctaagaaagc tgttgtcaag aaattgcaga aacactatgc
    12541 tcgcccagaa ggggttcctc tgatggagaa tggcccagag tttaagattg aggtctctat
    12601 tctcaaagat gtggcaactg tcatgattga tacgaccggg tctagcctct ttaaacgtgg
    12661 ttatcgtacc gaaaaaggtg gcgctcctat caaggaaaat atggcagcag ccattttaca
    12721 actttctaac tggtatccag acaagccttt gattgatccg acctgtggtt cggggacttt
    12781 ctgtattgag gcagttatga ttgctagaaa gatggcgcca ggtcttcgtc gctcttttgc
    12841 atttgaggaa tggaactgga tcagcgatcg cttgattcaa gaagtgcgca cagaagcggc
    12901 taaaaaagta gaccgtgagc ttgagctgga tatcatgggc tgtgatattg atgctcgcat
    12961 ggtggaaatt gctaaggcca atgctcaggc agctggtgtt gcaggagaca ttacttttaa
    13021 gcagatgcgc gtgcaggatt tacgttccga taaaatcaat ggagtaatca tttccaatcc
    13081 gccttatggt gaacgtttgt cagatgatgc aggggtgacc aagctctatg ctgagatggg
    13141 gcaagtattt gcaccgctga aaacttggag caaatttatc ctgactagtg atgaagcttt
    13201 tgaaagcaag tatggtagcc aagcagataa gaagcgtaag ttatacaacg gaaccttgaa
    13261 agtggatctg tatcaatatt ttggtcagcg tgtcaaacgg caagaggtaa aatagaaagg
    13321 gatactcatg agtaaaaaaa gacgaaatcg tcaaaaaaaa gaaggtcaag aaccgcaatt
    13381 tgattttgat gaagcaaaag agctaacagt tggtcaagct attcgtaaaa atgaagaagt
    13441 ggaagcagga gtcttgcctg aggattccat tttggacaag tatgttaagc aacacagaga
    13501 tgaaattgag gcggataagt ttgcgactcg tcaatacaaa aaagaggagt tcgttgaaac
    13561 tcagagtctg gatgatttaa ttcaagagat gcgtgaggct gtagagaagt cagaagcttc
    13621 ttcggaggaa gttccatctt ctgaagacat cttactaccc ttgcctctgg acgatgagga
    13681 gcaaggcttg gatcctctat tgctagatga tgaaaatcca acagaaatga ctgaagaagt
    13741 ggaagaggag caaaaccttt ctcgtctgga tcaagaggac tcagaaaaga aaagtaaaaa
    13801 aggctttatt ttgaccgttt tggcgcttgt atcagtaatt atttgtgtca gtgcttatta
    13861 tgtctaccgt caagtggctc gttcgaccaa ggaaattgaa acttctcaat caactacagc
    13921 caatcaatcg gatgtggatg attttaatac actttatgac gccttttaca cagatagcaa
    13981 taaaacggct ttgaaaaata gccagtttga taaactgaat caactcaaga ctttacttga
    14041 taagctggaa ggtagtcgtg aacatacgct tgccaaatct aaatatgata gtctagcaac
    14101 gcaaatcaag gctattcaag atgtcaatgc tcaatttgag aaaccagcta ttgtggatgg
    14161 tgtgttggat gccaatgcca aagccaaatc ggatgctaaa tttacggata ttaaaactgg
    14221 aaatacggag cttgataaag tgctagataa ggctatcagt cttggtaaga gccagcaaac
    14281 aagtacttct agctcaagtt caagtcaaac tagcagctca agttcaagtc aagcaagctc
    14341 aaatacgaca agtgagccaa aaccaagtag ttcaaatgag actagaagta gtcgcagtga
    14401 agtcaatatg ggtctctcga gtgcaggggt tgctgttcaa agaagtgcca gtcgtgttgc
    14461 ctataatcag tctgctattg atgatagtaa taactctgcc tgggattttg cggatggtgt
    14521 cttagaacaa attctagcga cttcacgttc acgtggctat atcactggag accaatatat
    14581 ccttgaacgt gtcaatatcg ttaacggcaa tggttattac aacctctaca agccagatgg
    14641 aacctatctc tttaccctta actgtaagac aggctacttt gtcggaaatg gcgctggtca
    14701 tgcggatgac ttggactact aagcagtcgt tacaaaattc tttcttttca aaagtaaaaa
    14761 tgataaaata aaacaaatga aacaagagga gtgtcaaatg acaaaagcta actttggtgt
    14821 cgtaggtatg gccgtaatgg gtcgtaacct tgcccttaat attgaatctc gtggttacac
    14881 agttgctatc tacaaccgta gtaaagaaaa aacggaagat gtgattgctt gccatcctga
    14941 aaagaacttt gtaccaagct atgacgttga aagttttgta aactcaatcg aaaaacctcg
    15001 tcgtatcatg ctgatggttc aagctggacc tggtacagat gctactatcc aagcccttct
    15061 tccacacctt aacaaggggg atatcttgat tgacggagga aatactttct acaaagatac
    15121 catccgtcgt aatgaagaat tggcaaactc aggtatcaac tttatcggta ctggggtttc
    15181 tggtggtgaa aaaggtgccc ttgaaggtcc ttctatcatg cctggtggac aaaaagaggc
    15241 ctacgaattg gtttcggatg ttcttgaaga aatctcagct aaagcaccag aagatggcaa
    15301 accatgtgtg acttatatcg gtcctgatgg agctggtcac tatgtgaaaa tggttcacaa
    15361 cggtatcgag tacggtgata tgcaattgat cgcagaaagt tatgacttga tgcaacactt
    15421 gctaggtctt tctgcagagg atatggctga aatctttact gagtggaaca agggtgaatt
    15481 ggatagctac ttgatcgaaa tcacagctga tatcttgagc cgtaaagacg atgaaggcca
    15541 agatggacca atcgtagact acatccttga tgctgcaggt aacaagggaa ctggtaaatg
    15601 gactagccaa tcatctcttg accttggtgt accattgtca ctgattactg agtcagtgtt
    15661 tgcacgctac atttcaactt acaaagaaga acgtgtatat gctagcaagg tgcttccaaa
    15721 accagctgcc ttcaactttg aaggagacaa ggctgaattg attgaaaaga tccgtcaagc
    15781 cctttacttc tcaaaaatca tttcatacgc acaaggattt gctcaattgc gtgtagcctc
    15841 taaagaaaac aactggaact tgccatttgc agatatcgca tctatctggc gtgatggctg
    15901 tatcatccgt tctcgttttt tgcaaaagat tacagatgct tacaaccgcg atgcagatct
    15961 tgccaacctt cttttggacg agtacttctt ggatgttact gctaagtatc aacaagcagt
    16021 gcgtgatatc gtagctcttg cggttcaagc tggtgtgcca gtgccaactt tctcagcagc
    16081 tattacttac tttgatagct accgttcagc tgaccttcca gctaacttga tccaagcaca
    16141 acgtgactac tttggtgctc acacttacca acgtaaagac aaagaaggaa ccttccacta
    16201 ctcttggtat gacgaaaaat aagtaggtca gccatgggga aacggatttt attacttgag
    16261 aaagaacgaa atctagctca ttttttaagt ttggaactcc agaaagagca gtatcgggtt
    16321 gatctggtag aggaggggca aaaagccctc tccatggctc ttcagacaga ctatgatttg
    16381 atgttattga acgttaatct gggagatatg atggctcagg attttgcaga aaaattgagt
    16441 cgaactaaac ctgcctcagt catcatgatt ttagatcatt gggaagactt gcaagaagag
    16501 ctggaagttg ttcagcgttt tgcagtttca tacatttata agccagtcct tatcgaaaat
    16561 ctggtagcgc gtatttcggc gatcttccga ggtcgggact tcattgatca acactgcagt
    16621 ctgatgaaag ttccaaggac ctaccgcaat cttaggatag atgttgaaca tcacacggtt
    16681 tatcgtggtg aagagatgat tgctctgacc cgccgtgagt atgacctttt ggcgacactt
    16741 atgggaagca agaaagtatt gactcgtgag caattgttgg aaagtgtttg gaagtatgaa
    16801 agtgcgaccg agacaaatat cgtagatgtc tatatccgct atctacggag caagcttgat
    16861 gttaaaggcc aaaaaagcta cattaaaacc gtgcgtggtg ttggatatac catgcaagaa
    16921 tagataagca gttgcagttg tgtaactgct tttttgagga gtttttatat attgacatgc
    16981 agtcgggtct ttgctacaat cgttttagga ggatatatag atgaaacttt tgaaaaaaat
    17041 gatgcaagtt ctactagcag tctttttctt tggtttgcta gctacaaata cggtatttgc
    17101 gaataccaca ggtggccgat ttgttgataa ggataataga aaatattatg taaaagatga
    17161 tcataaagca atctattggc ataaaataga cggtaaaact tactattttg gtgatattgg
    17221 agagatggtt gtcggttggc aatacttaga aattcctgga acaggttatc gtgataattt
    17281 attcgataac caaccagtta atgagattgg ccttcaggag aagtggtact attttggaca
    17341 agatggtgct ttgctagaac aaacagataa acaagtacta gaggcaaaaa cgtctgaaaa
    17401 tacaggaaaa gtatacggtg aacaatatcc tctatctgct gaaaagagaa cttattattt
    17461 tgataataat tatgctgtaa agacaggctg gatttatgaa gacggcaatt ggtattattt
    17521 aaataagcta ggaaattttg gcgatgattc ttacaatcca ctaccaattg gtgaagttgc
    17581 taagggttgg actcaagatt ttcatgttac tattgacatt gatagaagca aacctgctcc
    17641 atggtactac ctagacccag aaactggcat tatgcgaaca ggatggcaat atctaggtaa
    17701 taactggtac tacctccgtt catcaggagc aatggcaaca ggttggaaat atgtacgagg
    17761 caaatggtat tacttagata ataaaaatgg tgatatgaag actggttggt atcaagatgg
    17821 ttcaacttgg tactatttag atccttctaa tggagatatg aaaataggtt ggacaaaagt
    17881 aaatggaaaa tggtattatc tcaattcaaa tggagcaatg gttacaggta gtcaaactat
    17941 cgatggtaaa gtttataact tcgcttcatc tggtgagtgg atttaatgtt ggaggatata
    18001 taaaatgaag cttttgaaaa aaactatgca agctggacta acagtaattt tctttggttt
    18061 gctagctacg aatacagtat ttgcagataa ctctgaagga tggcagtttg tccaagaaaa
    18121 cggtagaacc tattacaaaa agggggacct caaagaaacc tactggcgag tgattgatgg
    18181 taagtactat tattttgatt ctctatctgg agagatggtt gtcggctggc aatatatccc
    18241 gtttccatct aaaggtagta caattggtcc ttacccaaat ggtatcagat tagaaggttt
    18301 tccaaagtca gagtggtact actttgataa aaatggagtg ctacaagagt ttgttggttg
    18361 gaaagaatta gagattaaaa ctcaagacag tgttggaaga aagtatgggg aaaaacgtga
    18421 aaattcagaa gataaagaag agaagcgtta ttacacgaac tattacttta atcaaaatca
    18481 ttctttagag acaggttggc tttatgatca gtctaactgg tattatctag ctaaaacaga
    18541 aattaatgga gaaaactata ttggtggtga aagacgtgca ggttggataa atgatgcttc
    18601 agcttggtac tacctagatc cagcaactgg tattatgcaa acaggttggc aatatctagg
    18661 taataagtgg tactacctcc gttcctcagg agcaatggcc actggctggt atcaggaagg
    18721 taccacttgg tattatttag accacccaaa tggcgatatg aaaacaggtt ggcaaaacct
    18781 tgggaacaaa tggtactatc tccgttcatc aggagctatg gcaactggtt ggtatcaaga
    18841 tggttcaact tggtactacc taaatgcagg taatggagac atgaagacag gttggttcca
    18901 ggtcaatggc aactggtact atgcttatag ctcaggtgct ttggcagtga atacgaccgt
    18961 agatggctat tctgtcaact ataatggcga atgggttcgg taatgaaaga agcgattgtg
    19021 aatggaaaca atcgcttttt ttgtgaaaat ataataaaat agataggaaa gaattaatac
    19081 ttgtatgaaa aataagacgt tttttcgtct agtaaaagga aaaaatgaca aaaaaagttg
    19141 gtgtcggtca ggcacatagt aagataattt taatagggga acatgcggtc gtttacggtt
    19201 atcctgccat ttccctgcct cttttggagg tggaggtgac ctgtaaggta gttcctgcag
    19261 agagtccttg gcgcctttat gaggaggata ccttgtccat ggcggtttat gcctcactgg
    19321 agtatttgga tatcacagaa gcctgcattc gttgtgagat tgactcggct atccctgaga
    19381 aacgggggat gggttcgtca gcggctatca gcatagcggc cattcgtgcg gtatttgact
    19441 actatcaggc tgatctgcct catgatgtac tagaaatctt ggtcaatcga gctgaaatga
    19501 ttgcccatat gaatcctagt ggtttggatg ctaagacctg tctcagtgac caacctattc
    19561 gctttatcaa gaacgtagga tttacagaac ttgagatgga tttatccgcc tatttggtga
    19621 ttgccgatac gggtgtttat ggtcatactc gtgaagctat ccaagtggtt caaaataagg
    19681 gcaaggatgc cctaccgttt ttgcatgcct tgggagaatt aacccagcaa gcagaagttg
    19741 cgatttcaca aaaagatgct gaaggactgg gacaaatcct cagtcaagcg catttacatc
    19801 taaaagaaat tggagtcagt agccctgagg cagacttttt ggttgaaacg actcttagct
    19861 atggtgctct gggtgccaag atgagcggtg gtgggctagg aggttgtatc atagccttgg
    19921 taaccaattt gacacacgca caagaactag cagaaagatt agaagagaaa ggagctgttc
    19981 agacatggat agagagcctg taacagtacg ttcctacgca aatattgcta ttatcaaata
    20041 ttggggaaag aaaaaagaaa aagagatggt gcctgctact agcagtattt ctctaacttt
    20101 ggaaaatatg tatacagaga cgaccttgtc gcctttacca gccaatgtaa cagctgacga
    20161 attttacatc aatggtcagc tacaaaatga ggtcgagcat gccaagatga gtaagattat
    20221 tgaccgttat cgtccagctg gtgagggctt tgtccgtatc gatactcaaa acaatatgcc
    20281 tacggcagcg ggcctgtcct caagttctag tggtttgtcc gccctggtca aggcttgtaa
    20341 tgcttatttc aagcttggat tggatagaag tcagttagcg caggaagcca agtttgcctc
    20401 aggctcttct tctcggagtt tttatggacc actaggagcc tgggataagg atagtggaga
    20461 aatttaccct gtagagacag acttgaaact agctatgatt atgttggtgc tagaggacaa
    20521 gaaaaaacca atctctagcc gtgacgggat gaaactttgt gtggaaacct cgacgacttt
    20581 cgacgactgg gttcgtcagt ctgagaagga ctatcaggat atgctgattt atctcaagga
    20641 aaatgatttt gccaagattg gagaattaac ggagaaaaat gccctggcta tgcatgctac
    20701 gacaaagact gctagtccag ccttttctta tctgacggat gcctcttatg aggctatgga
    20761 ctttgttcgc cagcttcgtg agaaaggaga ggcctgctac tttaccatgg atgctggtcc
    20821 caatgttaag gtcttctgtc aggagaaaga cttggagcat ttatcagaaa ttttcggtca
    20881 tcgttatcgc ttgattgtgt caaaaacaaa ggatttgagt caagatgatt gctgttaaaa
    20941 cttgcggaaa actctattgg gcaggtgaat atgctatttt agagccaggg cagttagctt
    21001 tgataaagga tattcccatc tatatgaggg ctgagattgc tttttctgac agctaccgta
    21061 tctattcaga tatgtttgat ttcgcagtgg acttaaggcc taatcctgac tacagcttga
    21121 ttcaagaaac gattgctttg atgggagact tcctcgctgt tcgcggtcag aatttaagac
    21181 ctttttccct aaaaatctgt ggcaaaatgg aacgagaagg gaaaaagttt ggtctaggtt
    21241 ctagtggcag cgtcgttgtc ttggttgtca aggctttact ggctctgtat gatgtttctg
    21301 ttgatcagga gctcttgttc aagctgacta gcgctgtctt gctcaagcga ggagacaatg
    21361 gttccatggg cgaccttgcc tgtattgtgg cagaggattt ggttctctac cagtcatttg
    21421 atcgccagaa ggcggctgct tggttagaag aagaaaactt ggcgacagtt ctggagcgtg
    21481 attggggatt ttttatctca caagtgaaac caactttaga atgtgatttc ttagtgggat
    21541 ggaccaagga agtggctgta tcgagtcaca tggtccagca aatcaagcaa aatatcaatc
    21601 aaaatttttt aagttcctca aaagaaacgg tggttttttt ggtcgaagcc ttggagcagg
    21661 ggaaagccga aaaagttatc gagcaagtag aagtagccag caagctttta gaaggcttga
    21721 gtacagatat ttacacgcct ttgcttagac agttgaaaga agccagtcaa gatttgcagg
    21781 ccgttgccaa gagtagtggt gctggtggtg gtgactgtgg catcgccctg agttttgatg
    21841 cgcaatcaac caaaacctta aaaaatcgtt gggccgatct ggggattgag ctcttatatc
    21901 aagaaaggat aggacatgac gacaaatcgt aaggacgagc atatcctcta tgcccttgag
    21961 cagaaaagtt cctataatag ctttgatgag gtggagctga ttcattcttc cttgcctctt
    22021 tacaatctgg atgaaatcga tctttcgaca gagtttgctg gtcgaaagtg ggactttcct
    22081 ttttatatca atgccatgac tggtggaagt aataagggaa gagaaatcaa tcaaaagctg
    22141 gctcaggtgg cggaatcctg tggtatttta tttgtaacgg gttcttatag cgcagccctc
    22201 aaaaatccaa cggatgattc tttttctgtc aagtctagtc atcccaatct cctccttgga
    22261 accaatattg gattggacaa acctgtcgag ttaggacttc agactgtaga agagatgaat
    22321 cctgttctat tgcaagtgca tgtcaatgtc atgcaggaat tactcatgcc cgagggagaa
    22381 aggaagttta gaagctggca atcgcatcta gcagattata gcaagcaaat tcccgttcct
    22441 attgtcctca aggaagtggg ctttggaatg gatgccaaga caatcgaaag agcctatgaa
    22501 ttcggtgttc gtacagtgga cctatcgggt cgtggtggca ccagctttgc ctatatcgaa
    22561 aaccgtcgta gtggccagcg tgattacctc aatcaatggg gtcagtctac catgcaggcc
    22621 cttctcaatg cccaagaatg gaaagataag gtcgaactct tggttagtgg aggggttcgg
    22681 aatccgctgg atatgattaa gtgcttggtt tttggtgcta agtctgtggg attgtcacga
    22741 accgttctgg aattggttga aacctacaca gttgaagaag tgattggcat tgtccaaggc
    22801 tggaaagcag acctacgctt gattatgtgt tcccttaact gtgccaccat agtagatcta
    22861 caaaaagtag actatcttct ttatggaaaa ttaaaagaag caaaggatca gatgaaaaag
    22921 gcgtaaccag cgcctttttt ccatcctcag actgaggtga cttttttgaa ttgtgataaa
    22981 atagaaggga gaggatgaac ctatgagaaa atttaaaatc tttttattta tcgaagcctg
    23041 tcttctgaca ggagctctga ttttgatggt atcagagcat ttttcgcgtt ttctgctgat
    23101 actattcctc tttttgcttt tgattcgcta ctacactggt aaagagggaa ataatcttct
    23161 tttagtagcg gcaaccattc tcttcttttt catcgttatg ctcaatcctt ttgtgattct
    23221 agctattttt gttgcggtta tctatagtct ctttcttctt tacccgatga tgaaccagga
    23281 aaaagagcag accaatttgg tttttgaaga ggtcgtgacg gttaagaagg agaaaaatcg
    23341 ttggtttgga aatcttcatc atttttcaag ctaccagact tgccaattcg atgatatcaa
    23401 tctctttcgc ttcatgggca aggacactat tcatctggag agggtcatct taaccaatca
    23461 tgacaatgtc attatcctca gaaagatggt aggaacgacc aaaatcatcg tacctgtaga
    23521 tgtggaagtc agtctcagcg ttaactgtct ctatggggat ttgacttttt tcaaccagcc
    23581 caagcgagcc ctccgcaatg aacactatca tcaagaaaca aaagactatc tcaagagtaa
    23641 caagagtgtc aagattttct tgaccactat gattggtgat gtggaggtgg ttagaggatg
    23701 aaaaaacaag cctatgtcat tattgctctc acctccttcc tatttgtctt ttttttctcc
    23761 cacagcttgc tggaaatact tgattttgac tggtctatct ttttgcacga tgtcgaaaaa
    23821 acagaaaaat ttgtcttttt attgttggtt ttcagcatgt ccatgacctg tctcttagcc
    23881 ctgttttggc gagggatcga agagctttct ctaagaaaaa tgcaggctaa tctcaagcgt
    23941 ttattggcag ggcaagaagt agttcaggtt gtagatccag atttggatgc cagtttcaag
    24001 tccttatcag gtaaacttaa ccttttgaca gaggctcttc aaaaagctga aaatcatagc
    24061 cttgctcagg aagaggaaat catcgagaag gaacggaagc gaattgctcg ggatttgcac
    24121 gatacagtca gtcaggagtt gtttgcggcc cacatgattt tatcgggtat cagtcagcag
    24181 gctttgaaat tggatagaga aaagatgcag atccagttgc agagtgtcac agctatttta
    24241 gaaactgccc agaaggattt gcgggttttg ctcttgcatt tgcgaccagt tgaactggag
    24301 cagaagagct tgatagaagg gattcagatt ctcttaaaag agcttgagga caagagtgat
    24361 cttagggtta gtctcaagca gaatatgacg aaattgccta agaaaatcga ggagcatatc
    24421 ttccgtatcc tgcaagagtt gattagcaat accctccgcc atgcccaggc atcttgccta
    24481 gatgtctacc tctatcagac agatgttgaa ttgcaactga aggtggtgga caatgggatt
    24541 ggtttccagt tagggagctt agacgacttg agttatggac tgcgaaatat caaggagcgg
    24601 gttgaagata tggcagggac ggttcagtta ttaacagctc ccaagcaagg gctggcagtt
    24661 gatatccgta ttcccctgtt agataaggaa tgataaagga gtaaagatga aaattttact
    24721 agtagatgac catgaaatgg tccgattggg cttgaaaagc tactttgacc tccaagccga
    24781 tgtagaagtt gtgggtgagg cgtccaacgg gtctcaaggg attgacttgg ccttggaact
    24841 gcgtccggat gttattgtta tggatattgt catgcctgag atgaatggga ttgatgcgac
    24901 cttggctatc ctcaaagaat ggcctgaagc caagattttg attgtgacct cttatttgga
    24961 caatgaaaaa atcatgccag tcttagatgc tggtgccaaa ggctatatgc tcaagacttc
    25021 tagtgcagat gaattgcttc atgccgtcag taaggtagct gctggcgagc tggccattga
    25081 gcaagaggtt agcaagaagg ttgaatacca ccgcaatcat atggaacttc atgaagaatt
    25141 gactgcgcgt gagcgagatg ttctccaact catcgccaag ggctacgaaa atcagcgcat
    25201 cgcagatgac ctctttatct ctctcaagac ggtcaagacc cacgtgtcca acattcttgc
    25261 caaacttgaa gtcagcgatc gtacccaggc ggctgtctac gcctttcagc accatttggt
    25321 ggggcatgag gagttttaga tgagtttagc agatttactt taggagctag aagcagcaaa
    25381 agactctaaa aagcaaggcc catggaagcc tatatgcgcc atcaattttc ttttctaggt
    25441 attgcggtgc ctgaaagaaa taaactctat aaaaatattt accagaagcg aaaaaaacaa
    25501 agattatcga ttgggatttt gcagacactt gctgggaaaa ggagcctaga aaataccaat
    25561 atgtggctgc taactatttg aaagcaatgc agtcttatct aaaggaaacc gatttgccta
    25621 agctagagca gttagtcgtg acaaaatcct ggtgggacac ggtagatatc ctagatcgag
    25681 tagtagggag tttggtgtat gaacaccctg aactagaaga aataatctta atactcttcg
    25741 aaaatctctt caaaccacgt cagctctatc tgcaacctca aaacagtgtt ttgagcaacc
    25801 tgcagctagc ttcctagttt gctctttgat tttcattgag tataaaatgg agcctatcag
    25861 ataatatctg gctgagacgt gtcgctattg accaccagtt gttaataaaa gagaagacca
    25921 atactcaatt aatggaaaag attctacttc ataacttgaa ccaaacagaa ttttttatca
    25981 ataaagccat tggctggact ctgcgagact actccaaaac gaatcccact tgggtaacat
    26041 gctttattga gaaaaacaag gaaagaatgg ctgaacttag tatcaaagaa gcaagtaagt
    26101 acctctagcg tgattgaaaa gcgcagtcat taattgaaaa gtttacctgt ttatgtgata
    26161 atggagggta tttaaagaag gtggtagatt tcttgattct atagtagatt aagaaattta
    26221 gaatagtacg ctataattac attaggagga tacaataatg aagaaaactg tttataaaaa
    26281 attgggtatt tcaattattg cgagtacttt attggctagc cagttatcga cagtatctgc
    26341 tttgagtgtt atttctagta caggtgaaga atatgaggta agtgagacac tagaaaaagg
    26401 tccagagtct aatgattctt cattatctga gatttcacca acgtatggtt catactacca
    26461 aaagcaatca gaagtattat cggtaatgat gatttgatta aaattgataa tactttacag
    26521 tatccgtatt cgacttcagc tatggtttta tctaagtatt atggagtagc cgatggaatg
    26581 aatgtagaag ggaggggaag tgcgaatttt attaaagata atgtgttaat tacagcggct
    26641 cacaactact acagacatga ctatgggaaa gaagcggatg atatttatgt tcttccggct
    26701 gttagtccaa gtcaagaact atttggaaag atcaaagtaa aggaagttcg ttatttgaag
    26761 gaatttagaa atttaaattc taagaatgca agggaatatg acttggcttt attaattcta
    26821 gaagagccca ttggtgcaaa attagggact ttgggtcttc ctactagtca aaaaaatttg
    26881 acaggaataa ttgtgactat cacaggctat ctatcatata attttaaaat tcatcaaatg
    26941 tatacagata aaaaacaagt tttaagtgat gatggcatgt tcttggatta ccaagttgat
    27001 actttagagg ggtctagtgg atctgcagtt tatgatgcta gtcaccgcgt agtaggagtg
    27061 catactttag gagatggagc taatcaaatt aacagtgcag ttaaattaaa tgaacgaaat
    27121 ttgccattta tttattcggt tcttaaaggt tactctcttg aaggatggaa gaaaataaat
    27181 ggtagttggt actattatag acaacatgat aaacaaacgg gttggcagga gataaatgat
    27241 acttggtatt atttagacag ttccggtaag atgcttacag attggcaaaa agtaaatgga
    27301 aaatggtatt atctcaattc aaatggagca atggttacag gtagccaaac tatcgatggt
    27361 aaagtttata acttcgcttc atctggtgag tggatttaat gttggaggat atataaaatg
    27421 aagcttttga aaaaaatgat gcaagtcgca ctagccacat ttttcttcgg tttgctaggg
    27481 accagtacag tatttgcaga tgattctgaa ggatggcagt ttgtccaaga aaatggtaga
    27541 acctactaca aaaaggggga tctaaaagaa acctactgga gagtgataga tgggaagtac
    27601 tattattttg atcctttatc cggagagatg gttgtcggct ggcaatatat acctgctcca
    27661 cacaaggggg ttacgattgg tccttctcca agaatagaga tttctcttag accagattgg
    27721 ttttattttg gtcaagatgg tgtattacaa gaatttgttg gcaagcaagt tttagaagca
    27781 aaaactgcta cgaataccaa caaacatcat ggggaagaat atgatagccc agcagagaaa
    27841 tgagtctatt attttgaaga tcagcgtagt tatcatactt taaaaactgg ttggatttac
    27901 gaagagggtc attggtatta tttacagaag gatggtggct ttgattcgcg catcaacaga
    27961 ttggcggttg gagagctagc acgtggttgg gttaaggatt cccctcttac ctatgatgaa
    28021 gagaagctaa aagcagctcc atggtactat ctagatccaa caactggcat tatgcaaact
    28081 ggttggcaat ttctaggtaa tagatggtac tacctccatt cgtcaggagc tatggcaact
    28141 ggctggtata aggaaggctc aacttggtac tatctagatg ctgaaaatgg tgatatgaga
    28201 actggctggc aataccttgg taacaagtgg tactatctcc gttcatcagg agctatggca
    28261 actggttggt atcaggaagg ttcgacttgg tactatctaa atgcaagtaa tggagatatg
    28321 aaaacaggct ggttccaagt caatggtaac tggtactatg cctatgattc aggtgcttta
    28381 gctgttaata ccacagtagg tggttactac ttaaactata atggtgaatg ggttaagtaa
    28441 tgaaggctaa ttgtaaactg tgatggatac ttaactttgt ataataggtg gataaaagtc
    28501 ttcacaatca aaaaacgcat agtatcaagg tttttctgta ctgcccccca aaagttagac
    28561 aaataattta tccaaaggat ttagttctgt actgcacagg actaagtcct tttagtttta
    28621 ccttaattct cttgttgttg taataatcaa tatagtctat aatagcttgt tccaattgat
    28681 taagtgattt aaatgttttc tcatagccat aaaacatttc cgatttcaga atcccaaaga
    28741 aggactccac catagtgctt ctctgtgaat gcctgttcca accttgtttg tacttgttct
    28801 aagttgggtg aagttgaaag attataggcg ataatttcac tgttaaagcc atctaaaact
    28861 ggtgataagt aaagcttttg agtacttgct ggaatggcaa attctgtcac atctgtgtag
    28921 cacttttcca ttgttttaga gccttcaaat tggccttgaa tgagattctc tgccttctta
    28981 ccaacgtctc ctttattttc gtttctgtcg cattttagct tgtaaattga gtactttcat
    29041 caagccttga actcttttat gatttaccag ataaccacga tttcttagtt ctaaatgaac
    29101 ccggcgataa gtataatttc ccttgtgttc gataaaaatg gattgaattt cagctttaag
    29161 ctcttggtcc ttatctggtt tgtctagctg tttcaagtga tagtagtagg tccaacgagc
    29221 tagtttaatg gcttttagaa gaagatctaa cgaaaactca gtcattaatt cttgaacaat
    29281 ttctatcttt cttctttctc tttttcctcc ttcaatcgga gttctcttaa cttttttagg
    29341 atagcattct ccgctctcag gtactctctc tcttgttttc tcaacaatag tatacccgtt
    29401 tttcctgtat tgtgctagcc agttaagaag tatcgtacga cttgggaggc agtattcaag
    29461 agaaactcta tctttagtcc agccttcatg tcagacttta tgaatcattt cttgttttaa
    29521 atcaggagaa tagtaacgat ttttcccttt tttgacgaac tctattccgt aacgatcaat
    29581 caatttaatc atatacctaa gattagaatt gtttatccca aatttatttg aaagcttctc
    29641 taagttatat ccttgttttc taagttcata gatctgaact ttatcatcat aagttaattt
    29701 cataataaaa acaccccaaa agttagattt tttctgtcta acttttgggg tgtagttcat
    29761 gtacaccgga tatgatgcgt tttatcattt ttaagccttt ttgcccagcc tcgtcaaaag
    29821 taatgttttg acacaaaatt tgtgacaaaa ctttagtttt aaaggttttt aactttgtat
    29881 atactagttt taagaaaagg aggatgatct aatggaagaa aaagtatcat tgaaagtcag
    29941 ggttcaaaaa ctagggacat cgctttcaaa tatggttatg cccaatattg gagcatttat
    30001 tgcttgggga gtattgactg ccctctttat cgctgatggc tatctgccaa atgaacagtt
    30061 agctactgtt gttggtccta tgttaacgta tttattgcca atcctgattg gttacacagg
    30121 tggatatatg atccatggcc aacgtggtgc cgttgtagga gctattgcta ctgttggtgc
    30181 aatcacaggt tctagtgttc ctatgtttat cggagctatg gtaatgggcc cactgggagg
    30241 atggactatc aagaaatttg atgagaagtt ccaggaaaaa attcgtcccg gatttgaaat
    30301 gttagttaat aacttctcag ctggtctcgt tggttttgca ttattgcttt tggctttcta
    30361 cgcaatcggt ccagtcgtat cgactcttac tggagctgtt gggaatggtg ttgaggctat
    30421 tgtcaatgct cgcctccttc ctatagctaa tattatcatc gaaccggcta aagtcctttt
    30481 cctcaataat gccctcaatc atggcatttt tactcctctg ggagtagaac aggtagctca
    30541 agctggtaag tcaattctct tcctattgga agctaatcct ggaccaggcc tgggaattct
    30601 attagcttat gctgtattcg gtaaaggttc tgctaaatct tcttcttggg gggcaatggt
    30661 tattcatttc ttcggaggga ttcatgaaat ttactttcct tatgttatga tgaagcctac
    30721 tctattttta gctgctatgg caggaggtat ctctggaact tttacttttc aactcttaga
    30781 cgctggtctt aaatctccag cttcaccagg ttctattatt gcgattatgg ctacggcgcc
    30841 aaaaggtgtt tggccccatc taaatgttct tctaggtgtt ttagtggcag cagttgtttc
    30901 tttctttgta gcagccctta ttcttcatgc agacaagtca actgaggatt cgctcgaagc
    30961 tgctcaagag gctacccaag cagctaaagc tcagtctaaa ggtcagttag tatcaacttc
    31021 tgttgatgca gttatttcga cagactcagt ggaaaaaatc attttcgcct gcgatgctgg
    31081 tatgggaagc tctgctatgg gagctagtat tcttcgagat aaggttaaaa aagcaggtct
    31141 agagattcca gtatctaatc aggcaatctc aaatttgctt gatacaccaa aaacattaat
    31201 tgttactcag gaagaactga caccaagagc taaagacaag agtccaagtg ctattcatgt
    31261 ttctgttgat aatttcttag cgtcccctcg ttatgatgaa attgtagctt cattaacagg
    31321 agcttttcca atagcagaaa ttgaaggaga tataccaact tcagcaccag tagatagtca
    31381 ggaaattgac cttaaccata ttgatgctgt agtagttgct tatggtaaag cacagggaac
    31441 tgcaactatg ggctgtgaaa cgattcgggc tatctttaga aacaagaata ttcgtattcc
    31501 agtttctact gccaaaattt cagaattagg tgaatttaat tctaaaaaca taatgattgt
    31561 aacaactatt tctttacagg cagaagtgca gcaagcagca ccgaattctc aatttcttat
    31621 tgtggatagt ttagtaacaa caccagaata tgacaaaatg gctgctagaa tgtacaaata
    31681 gaactagagg tttctaaatt acgaatgcta ttaaccaaac gagaagaaca attattgaag
    31741 gctttcctac atgtagggaa gctttcaatg caagatatga ctgaaatctt acaggtttca
    31801 tctagaacaa tttatcgaac tttatcagat ttgacagata gcatggagca atatggaatc
    31861 gaaataacga agcatgggaa atactatatt ttgactggag agttggatga tttgccgaca
    31921 gaacttgaag tgttagttga gtatagtccc caagaaagac aagagttgat tacctatcgc
    31981 cttctgactg agagtggttt tgtcaccaat gaagcattgc aagagtgcac gaaagtcagt
    32041 aatgtaacta ttattcagga tatttcagat attgataagc gtcttttaga ctttgatctg
    32101 aaaattgaac gacaaaaagg ttatcggatt tctggtgatt cagttggtaa gagaagattt
    32161 ttggctattt tactgacaaa ctgtatctca gtagcagatt tttcaaccgg taattttggg
    32221 agctttgata ttttagaagc agatagaact aggctggcca gtcagattgt taataagcaa
    32281 ctgtcaggtt ttccagatat ggatgctagg atgaagatgt tttttgcgat cttgttatct
    32341 cttataggtc aggagcaaaa cattgaaaat tcacctaata ctagtaagca ggctttggaa
    32401 atttctcaaa aaatttttca agcttactct aagcagactg cacaatttta tagtattcag
    32461 gaaattatct attttgcgag catcttggat gaattaatca ttaaacgtca ggacaatccg
    32521 ctctttacgg agaaatttga tggtgaattt ttctacaata tttcaaatct gattgatacg
    32581 gtttccatgt ataccaagat tgactttttt aaggacaagg ttttattcaa ttttcttttc
    32641 catcatattc ggctcagttt aggcgtccct atcctttttc agggtgaaaa tttgccagaa
    32701 tctgtccaga ttttagttga aaggaataaa tttctttata cagtcatcag tcttttagtg
    32761 aatgatattt ttccgaaata tcttcataca gagtatgagt atggcatgat tgccctacat
    32821 tttatctcta gcttaggccg tagtccagag atttatccag tccgtgtttt gcttttaacg
    32881 gatgaacgtc gggtcactag agatttatta gtcagtaaaa ttaagagtgt tgctcctttt
    32941 gtagagttga tagatattca gtctctagta gattaccaca gtattgatct cagtcagtat
    33001 gattatattt tatctaccaa gccgctgact aatcaggaaa tcgatgtaat ttctagtttt
    33061 ccaaccgtca aagaattgct tgaattacag gaacgacttc agtatgtaca ggcacatcgt
    33121 acaattgtcg cgcgtgatgc tatcgctcca gagaaaagtt atgacttgca agattattta
    33181 atatctagta gtcagctttt gagtcaattc gagttggttc aattggagaa taatcaatca
    33241 tttgagcaca cggtagaaca aatcatccaa tatcagaaga atgtgagtga cagagattac
    33301 ctaacaagaa aattgttatc tcacttccag aatagtccta tggctattcc taatactggt
    33361 ctggtgcttt tacatagtca gtctagcaaa gtaacaacaa atagttttac tatgtttgaa
    33421 ctcaaactac ctatctccgc attgtcaatg aaacgagagg aagaagaggt caaaaggtgt
    33481 ctgctaatgc taatgtctaa agaagctagc gaggaagcga gagatttaat gacagctatt
    33541 agtcagtcga ttattgaaaa tcatctttat acagagattt acaagacggg aaatcaatcc
    33601 attatttatc agatgctaaa tactattttt aacgaaaaaa ttaagaaatt ggagaactaa
    33661 tatgaaactt gaaaaacatt tgattaagct taataaacaa ttttctaaca aggaggaagc
    33721 tatttgttat tgtgggcaag ttctttatga gggtggatat gttaatgaag actatattga
    33781 agccatgatt gagcgagata aagagctatc tgtttacatg ggtaacttta tcgcaatacc
    33841 gcatggaaca gatgcagcaa aaaaggatgt cctcaagtct ggtattacag tcgttcaagt
    33901 ccctagaggg gttgattttg ggaatgtatc taaccctcaa gtggcaacgg ttctttttgg
    33961 tattgctggt attggtaatg aacacttaga aattattcag aaaatttcta tcttctgtgc
    34021 agatgtagat aatgttctta aattagccga tgctcagtca aaagaggaag tattgcgctt
    34081 atttgatgct gttgaataat tgaatttcgt catttgccat ctagtatata tgtccctcaa
    34141 ataggaaaag gagaaattga atgaaacatt ctgttcattt tggtgccggt aatatcggtc
    34201 gtggttttat aggtgaaatt ctatttaaaa atggtttcca tattgatttt gtggatgtca
    34261 ataatcagat aattcatgct ctaaatgaaa agggcaagta tgaaattgaa attgcacaga
    34321 aaggacagtc tcgtatagaa gtaactaatg tggctggaat taatagcaaa gaacatcctg
    34381 agcaagtcat tgaagcgatt caaaagacgg atattattac tactgcaatc ggacctaata
    34441 tactcccttt tatcgctgaa cttctagcca aaggaatcga agctcgccga gttgcaggga
    34501 atacacaggt attggatgtt atggcctgtg aaaatatgat tggcgggtct caatttcttt
    34561 atcaagaagt caagaaatat ttaagtccgg aaggtttgac atttgctgat aactacatag
    34621 gttttccaaa tgctgcagta gacaggattg ttccagcaca aagtcacgaa gattcccttt
    34681 ttgttgtggt cgagcccttt aatgaatggg tcgtggaaac caagcgtctt aaaaatccag
    34741 atttacgtct aaaagatgtg cattatgaag aagatttaga accctttatt gagcgaaaac
    34801 ttttttcagt caattctgga catgcaactt cagcttacat tggtgcgcat tatggtgcca
    34861 agacaatttt ggaagctctt caaaatccta atattaaatc tcggattgaa tctgtattag
    34921 ctgaaattcg gagtctcttg attgccaaat ggaactttga taaaaaagaa ttggagaatt
    34981 atcacaaagt cattatagaa cgatttgaaa accctttcat agtggacgag gttagtcgcg
    35041 tagctcgtac tccaatccga aaattaggct ataatgaacg attcatccgg ccgatacgtg
    35101 aattgaaaga actcagtttg tcatataaaa acctacttaa aacagttggc tatgcctttg
    35161 actatcgcga tgtaaatgat gaagaaagta ttcgattagg tgaattgttg gctaaacaat
    35221 cagtcaaaga tgttgttata caagttacag gtttagacga ccaagaattg attgagcaaa
    35281 ttgtagagta tatttaatat ttttcgaaaa tctcttcaaa tcaggttagc atcgctttgt
    35341 cttaggcata tgttactgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag
    35401 ctgactccgt cagttctatc t
//
LOCUS       DAVMAF010000014        34763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVMAF010000014 DAVMAF010000000
VERSION     DAVMAF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.014"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            68..523
                     /locus_tag="VYO97_000674"
     CDS             68..523
                     /locus_tag="VYO97_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1753509.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYSNPAYFY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            688..987
                     /locus_tag="VYO97_000675"
     CDS             688..987
                     /locus_tag="VYO97_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1753510.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1247..1570
                     /gene="tnpB"
                     /locus_tag="VYO97_000676"
                     /pseudo
     CDS             1247..1570
                     /gene="tnpB"
                     /locus_tag="VYO97_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2299..4026)
                     /gene="ezrA"
                     /locus_tag="VYO97_000677"
     CDS             complement(2299..4026)
                     /gene="ezrA"
                     /locus_tag="VYO97_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1753511.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(4108..6054)
                     /gene="gyrB"
                     /locus_tag="VYO97_000678"
     CDS             complement(4108..6054)
                     /gene="gyrB"
                     /locus_tag="VYO97_000678"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1753512.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGYTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(6069..6632)
                     /locus_tag="VYO97_000679"
     CDS             complement(6069..6632)
                     /locus_tag="VYO97_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1753513.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(6717..7271)
                     /locus_tag="VYO97_000680"
     CDS             complement(6717..7271)
                     /locus_tag="VYO97_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1753514.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(7282..8505)
                     /locus_tag="VYO97_000681"
     CDS             complement(7282..8505)
                     /locus_tag="VYO97_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1753515.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(8542..10992)
                     /locus_tag="VYO97_000682"
     CDS             complement(8542..10992)
                     /locus_tag="VYO97_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1753516.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVFLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(11192..12121)
                     /locus_tag="VYO97_000683"
     CDS             complement(11192..12121)
                     /locus_tag="VYO97_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1753517.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQVFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            12261..12548
                     /locus_tag="VYO97_000684"
     CDS             12261..12548
                     /locus_tag="VYO97_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1753518.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(12582..13916)
                     /gene="ciaH"
                     /locus_tag="VYO97_000685"
     CDS             complement(12582..13916)
                     /gene="ciaH"
                     /locus_tag="VYO97_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1753519.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(13906..14580)
                     /gene="ciaR"
                     /locus_tag="VYO97_000686"
     CDS             complement(13906..14580)
                     /gene="ciaR"
                     /locus_tag="VYO97_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1753520.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(14689..17235)
                     /locus_tag="VYO97_000687"
     CDS             complement(14689..17235)
                     /locus_tag="VYO97_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1753521.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISAFERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(17358..17798)
                     /locus_tag="VYO97_000688"
     CDS             complement(17358..17798)
                     /locus_tag="VYO97_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1753522.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(17773..18642)
                     /locus_tag="VYO97_000689"
     CDS             complement(17773..18642)
                     /locus_tag="VYO97_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1753523.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTTPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(18620..19120)
                     /locus_tag="VYO97_000690"
     CDS             complement(18620..19120)
                     /locus_tag="VYO97_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1753524.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(19131..19829)
                     /locus_tag="VYO97_000691"
     CDS             complement(19131..19829)
                     /locus_tag="VYO97_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1753525.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPSGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(19822..20088)
                     /locus_tag="VYO97_000692"
     CDS             complement(19822..20088)
                     /locus_tag="VYO97_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1753526.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(20154..21080)
                     /locus_tag="VYO97_000693"
     CDS             complement(20154..21080)
                     /locus_tag="VYO97_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1753527.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(21155..21970)
                     /locus_tag="VYO97_000694"
     CDS             complement(21155..21970)
                     /locus_tag="VYO97_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753528.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVILGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKETVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(21974..22294)
                     /locus_tag="VYO97_000695"
     CDS             complement(21974..22294)
                     /locus_tag="VYO97_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1753529.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(22501..22581)
                     /locus_tag="VYO97_000696"
                     /pseudo
     CDS             complement(22501..22581)
                     /locus_tag="VYO97_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(22625..24622)
                     /gene="metG"
                     /locus_tag="VYO97_000697"
     CDS             complement(22625..24622)
                     /gene="metG"
                     /locus_tag="VYO97_000697"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1753530.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(24746..26005)
                     /locus_tag="VYO97_000698"
     CDS             complement(24746..26005)
                     /locus_tag="VYO97_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753531.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYEFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(26007..26708)
                     /locus_tag="VYO97_000699"
     CDS             complement(26007..26708)
                     /locus_tag="VYO97_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753532.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(26692..27891)
                     /locus_tag="VYO97_000700"
     CDS             complement(26692..27891)
                     /locus_tag="VYO97_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1753533.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            28156..29502
                     /gene="gor"
                     /locus_tag="VYO97_000701"
     CDS             28156..29502
                     /gene="gor"
                     /locus_tag="VYO97_000701"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1753534.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAQAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            29699..30235
                     /locus_tag="VYO97_000702"
     CDS             29699..30235
                     /locus_tag="VYO97_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1753535.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            30286..30372
                     /locus_tag="VYO97_000703"
                     /pseudo
     CDS             30286..30372
                     /locus_tag="VYO97_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30441..30656)
                     /locus_tag="VYO97_000704"
     CDS             complement(30441..30656)
                     /locus_tag="VYO97_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1753536.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            30754..31074
                     /locus_tag="VYO97_000705"
     CDS             30754..31074
                     /locus_tag="VYO97_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753537.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(31104..31442)
                     /locus_tag="VYO97_000706"
     CDS             complement(31104..31442)
                     /locus_tag="VYO97_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753538.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(31454..32173)
                     /gene="trmD"
                     /locus_tag="VYO97_000707"
     CDS             complement(31454..32173)
                     /gene="trmD"
                     /locus_tag="VYO97_000707"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1753539.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(32163..32681)
                     /gene="rimM"
                     /locus_tag="VYO97_000708"
     CDS             complement(32163..32681)
                     /gene="rimM"
                     /locus_tag="VYO97_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1753540.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(32712..33500)
                     /locus_tag="VYO97_000709"
     CDS             complement(32712..33500)
                     /locus_tag="VYO97_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1753541.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNRHYLQWSESEKIAAIIRELSE"
     gene            complement(34077..34316)
                     /locus_tag="VYO97_000710"
     CDS             complement(34077..34316)
                     /locus_tag="VYO97_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1753542.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(34336..34608)
                     /gene="rpsP"
                     /locus_tag="VYO97_000711"
     CDS             complement(34336..34608)
                     /gene="rpsP"
                     /locus_tag="VYO97_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1753543.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
BASE COUNT        10349 a         7549 c         6215 g        10650 t
ORIGIN      
        1 ataccacatt gtgtactatt tttggttcat ttcactataa taataacgat actagataat
       61 gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa gcgaatacag actatattgc
      121 tcgctatggc gtatatgcag ttatccctaa tcctgaacaa aaacaaattg ttcttgttca
      181 agaaccaaat ggtgcttggt tcctaccatg tggagaaatt gaagcaggtg aaaatcatca
      241 ggaagcccta aagcgtgagt tgattgaaga gcttggtttc acagcagaaa ttggtaccta
      301 ttacggacaa gctgacgaat atttctattc tcgtcatcgt gatacctact actccaatcc
      361 tgcctacttc tatgaagcaa ctcctttcaa agaagtacaa aagccactag aaaactttaa
      421 tcatattgcc tggttcccta ttgacgaggc tatcaaaaac cttaaacgtg gtagccataa
      481 atgggccatt gaatcttgga aaaaacagca taagattgac taatactctt cgaaaataaa
      541 attcaaacca catcagcgtc gccttaccat actcaagtat agcctgcggc tagcttccta
      601 gtttgctctt ttattttcat tgagtataaa tcaagcgact ttattcaaaa agtgctataa
      661 tagaattaga aaaccggagg aaatattatg aagcttatca atacgacaaa ctcacactcg
      721 caacttgtaa aaagtcagct agaaagtacc gatgcaaccc ttgttgaggt ttattctgca
      781 gggaatacag atgttatttt tacccaagct ccgcttcact atgaaatcct catctcaaac
      841 aaacaccgtg ctattcgtga gcctgaaatc gaaaccattc aagaattttt cttgaaacgt
      901 aaaattgata agacctctgt tgatgaagcc aatatcaaga cactctactc agagaaatta
      961 attggaattt caatcccaat caagtaagaa aattgagtaa aaactcgatt tgagagaaag
     1021 tccaataagt ctttccataa caaaacgcat agtaacaaga gtttctacac ccactattat
     1081 gcgtttttat cattttgaaa tctactgcaa tcaatgaaga agcaaagatc aaactaggca
     1141 gctagttaca agttacgtaa gcgcgtcata acaaggtatc tatcctaatt ccccaacttc
     1201 cagtatacta gataaaaaat aattatcgga ggaaaggtat tgtactatga taattcaact
     1261 aagtgattta ggtcaagttc accttgtttg tggtaagaca gatatgaggt agggaataga
     1321 ctcattagcc tatgtagtta aaacccactt tgaattggat tctttctccg gtcaagtctt
     1381 tctcttttgt ggtggacgta aagaccgctt taaagccctt tactgggatg gtcaaggatt
     1441 ttggctacta tataaacgct ttgagaacgg aaaactgact tggctaagta cagaaaagga
     1501 tgtcaaagct ctcgcacctg aacaagtaga ttggctgatg aagggctttt ctatcactcc
     1561 aaaaatatag tagattgaaa ctagaatagt acacctctgc ttctaaaata ttgttagaaa
     1621 tcgatttgac tgtcctgatc gatttgtcct gttcttgttt cgttttacta tagaacgatt
     1681 tgaaggcgtt tatgatattt agctgtacga gagtctttta aaagtgtttt gatggtttgg
     1741 atttcttctt taattgattt catattacta ttgtataatg ctttttgatt ttagtctagt
     1801 ataaatagcg agaaatatct atcctatctt ctagaatgtc ttccaaacga gaaaactctc
     1861 gtaaacaaag aggttttaga ggcctattta ccgtggacta aagttgtaca agaaaagtgc
     1921 aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga gatttttcaa
     1981 tagacttcgt tattggacgg ttaccaagtt actcaaagta cagatttgaa gctgcagata
     2041 agactgacga aatcacttta atactaaatg aaaatcaaaa agcaaactaa gaagctagcc
     2101 tcaggctgta cttgagtacg gtaaggcgac gctgacgtga tttgaagaga ttttcgaaga
     2161 gtataaaaca ctgttttgag gtggtggata aatcggctgg gtaaaaactc aatctcagga
     2221 gaaaatgaag taaatctccc cacaactata gaaaaagatt tgattctgct cctcacacaa
     2281 taaaatcttt ttcttttatt aaaaacgaat cgtttcacgt gttttctcat atgaggtaac
     2341 aaagcgattg gttacaccag gctctgccac ttccaatgct tgagaaatct tgtcaaatga
     2401 agcgtgataa tcaaattctt tttcaaaaat atctaaagct tcgttaaatg cttcttgaat
     2461 gcgttcatca aatgagcgat agcggttaga atattgcaag agttgctctg tcaaagttgc
     2521 atattgtaca atattataag tttccgtttc taaagcttcc atatcattcg ttgcaatttc
     2581 aagaactcgg gtaacagatt caatgttaat cattttttgt tctaactcaa ccattaaatc
     2641 ctcggtatta ttgcttgccg taaagaataa cttcaagaaa gtttgtggaa tacctggcag
     2701 attgcgtttt tccatgtatc gcttgatagt atggagacga ttgacataaa cattggcctt
     2761 ttgacgtgca ttaatatcat ctttctcaat ttgtgtcagg cgctcactaa ctgaaatttg
     2821 ctcatcttca atatctttta gttgagtttg taaatcctca agattttctt caagaactga
     2881 ataagcttgg gttggttctt cttgatttga agttacctca acaatagctg cctcaaaact
     2941 ctctaattct gtctgaatac gacgaacatg gcttgcagct gtctcaggaa gtaaataggt
     3001 cttgttcaaa cgtgcaatat cttctcccaa taaagtatta ttctctttca tatgttgaag
     3061 gtaagttgga agagttgcaa gtagattttc cactactttc tgagcagcaa tttctcgagt
     3121 aaaaatatca tacaaggcat tgatttcctc ttgtgcctgt ccattctcat attcggcatt
     3181 atccaattcc aactgacgaa tattctcttg gtttttcttg aatgcttcat aaagcaagtg
     3241 gaaacgcgct tcaatatccg tttcaacaaa atgataatta gcatcaatta gtttacgata
     3301 accggattcc aaatcctgta attgatctgg caattctgta gaaagcgtcg taaccaaggc
     3361 tggaacacga tccacaatat gacttaaggc caaaatgtga ttttctgtat tatccaaaat
     3421 cactgcggct tccacagggt cacccgatga attcaaggtt acaaattgtg aaaattcaga
     3481 ttggatattt tctaattgtt tttcaatttc atccaaggct tgaccatact gttctgaatt
     3541 ttcagcaact ctatgctgaa gttcctcaaa taaatccaaa gcatgaagaa cacgaccact
     3601 atttttagat tcttgcttct ctaagtctgc caaagcattg cgaattgccg caatatcttc
     3661 ttcaatcaaa gtaatttgac tctcaatttg gtcaatttga tgactggcct tgagaaaacg
     3721 aaatgaatgg ttataacctt ctgcttcaaa gagattattt tcaatatcgg caaaagagtt
     3781 gagagataaa tcgacccatt tttgattcca ttcacggaaa gccacttgac tttgtccaat
     3841 caagtgcata ttttttacag cttctacttc atcatttact ggaagattgt atagttcttc
     3901 ttttctttct tctagcgcct ctaatctccc ctcgtttcgc ttacgtagaa agattgccac
     3961 tacataagcc agaactaaaa tgactgcaat tgcaaccatt aaataaatta gttgtccatt
     4021 agacatatca aactcctttt ttacttgaaa caatcgtaac gattatatca tattttttag
     4081 accaagggaa ctacttctcc ctatttttta gacatcaagt gtactataga cagcattttc
     4141 ttcgataaac tcacgacgag gctctactcg atcccccatc aacatatcaa agattttatc
     4201 tgcttctgca gcatcatcta cagaaactct agccatcaag cgatgttcgg gatccatggt
     4261 tgtttcccac agctgatgat cgtccatttc acctagcccc ttataacgct gaatagtcgg
     4321 tttggtacga ccttcactat aacgggctaa agcttcttgg agtttgattt cttgatctgc
     4381 acccggctgg atatattctt taatctcgct tccaaccttg acaccataga ttggtggttg
     4441 ggcaatataa acataaccag cttctaggat tggtttcata taacgataaa tcaaggttaa
     4501 aagaagggta cgaatgtggg ctccatcgac atcggcatcg gtcatcaaaa cgagtttttg
     4561 gtaacgggct ttcgaaacat caaattctgc gccaaatcct gttcccatgg ctgtgaaaag
     4621 actacgaatt tcttcgttgg ctagaatctt atccatactt gctttttcaa cattcaaaat
     4681 cttaccgcga attggaagga tagcctgaaa ctcacggtta cgaccagatt tggctgatcc
     4741 accagctgag tctccttcga cgatgaagag ttctgtttca gcagggttat tagaagaaca
     4801 gtctgctagt ttccctggaa ggttggaaat ttccaaacca gattttttac gtgtgacttc
     4861 acgcgcacgc ttggcagcca cacgagcctt ggcagccaaa attccttttt ctacgatacg
     4921 tttggcaatc tgtggatttt ccatgaggaa atcggagaag gcttcactga agaggcgatt
     4981 ggtaatcttg accacttcgc tatttcccaa tttggtcttg gtttgtcctt caaactgtgg
     5041 atttgggtgt ttaactgaga taactgcagt taagccttcg cgaacatctt cccctgttaa
     5101 attatcttca ttgtctttca gtaacttatt cttacgagca taatcattga taacacgagt
     5161 cagtgccgta cggaaacctt gttcatgcgt tccaccttca tgggtatgaa tattattggc
     5221 gaaactcatg acattttcat ggtaacctgt tgtgtactgc atggctacct caactgtgat
     5281 atcatccatc tcaccgtctg tatagattgg tgtatcaaag attacatcct tgttctcgtt
     5341 gatatattca acgtaactag caatcccacc ttcataatga taatgcttgg tttgttccaa
     5401 accttggcgc ttatctgtaa ttgaaatttg aagaccgcga tttagaaagg ccaactcttg
     5461 aatccgttta tttaatttat caaaatcaaa gattgttgtt tcagtgaaaa tttctgggtc
     5521 cggtgtgaag tgaaccgttg ttccagttct atctgtatac ccaaccacct caaggtctgc
     5581 aacaacatga ccacgacgat attcttggta atgaatctta ccgtttttat ggacatgaac
     5641 gtctaattga gtggaaaggg cattaactac tgacgacccc accccgtgaa gaccacctga
     5701 aaccttgtat ccaccaccgc cgaactttcc tccagcatga aggactgtaa agacggtctc
     5761 aacagcagga cggcctgttt tttcctgaat atcgactggg ataccacgcc catcatccac
     5821 aacagtaatc gaatcatctg gctcaataaa aacttgaata tggctggcaa atcctgccaa
     5881 ggcctcgtca attgagttat caacaatttc ccagactaga tggtgaagac cttcttttga
     5941 ggttgatcca atgtacatcc ctggacgcat acgaacagcc tctaagccct ctaaaacttg
     6001 aatttgactg gcatcataat cctgtgcctg cagatttttg atttcttctg tcatcttatt
     6061 cctttttctt atatgtctaa tacttgtctt aaattcacga tactggtaaa caagtgggta
     6121 tcaagtcctg cagcttgacc tgcttcgata tcaatcggcc gatcaccaat gacaagacca
     6181 gagctaatct gatacttttc tcttaaataa agcatggatt cgggatttgg ctttctctta
     6241 aagcctgaac tagaagtcac cacttctgta aaataagctg ctatagaggt tttttctaaa
     6301 atttccaaaa cctgatcatt tcgatgagag accaaaaaat gacggccacc ttgatttaaa
     6361 atgtcttcca ataggtcaga aactccttca aataaaatcg ggtgttcaag ctctctggct
     6421 tcattttcct tgtacttttc taaaaaattc tctaaattgg gagcgaatgt ctcaatcgca
     6481 aaaggagtag aaacctttaa agcttgatag acactgtcat ggtcttgtgt gataccatac
     6541 agtgccaatg tttcaacaaa tgcagctgtt gaagtttcat aattatccag taaagttcca
     6601 cctaaatccc agatgtaatc gtgatatttc atacccttca ttatagcatt tttttatgaa
     6661 aaaagcgatg atttccctga gttttccaca taaaaaacga gagaataact cccgttttac
     6721 tgatttttac caaagacatc tcgatagagc attcctgttc gtaaactctc tgcgtcccct
     6781 cctagttgct ccaccaactc gtaggcaaag gcaagggctg ttgaaggacc ccgactggtt
     6841 gtcaactgac catctaccac tactgtttcc ttgacgtagt gaccatcaag gatttgctct
     6901 tgaacgccgt cataacaagt gtatcgctta tttttcaata tctctgcttg attgagggca
     6961 attggtgccg cacaaatggc tgctagtttc ttcccttctt gctcgaagct ttgcaattct
     7021 tgaatcaagg tctgattatc acgtaaatgt gcagaaccag gcatacctcc aggaagaaca
     7081 atcatatcat agtctgataa atctccatca aagacatgat ctgctcttac ttggattgca
     7141 tgcgaacccg ttacttgctc ttcaaaacca accatatcac atgtgatatt ggctcgacgc
     7201 aagacatcta caactgtcaa ggcttcaatt tcttcaaagc cctgagctaa cataactgct
     7261 acttttacca tgattttctc cttatttaat ttgttttaat acaacctttt tccgtttgaa
     7321 tgggactttt ccgctctttt cttcagctag attagtctgg taactcatcg ataaaagcaa
     7381 accaacacca atcagattac tgataatagc tgatccccct tgcgaaatga aaggcaaggg
     7441 aatccccgtc aaaggaagta gtccagtcac agcaccgata ttctcaaaga tgtggaagag
     7501 caacatcata atcaaacctg tggaaatata agtgtagaac tggttatttg atttaagagt
     7561 aatcttcaac atacggtaaa tcaacatgag atagagggca ataaccagga cagagccaat
     7621 aaagccaaaa tcttctgcaa taaccgtaaa aatcatatct gactctcgaa ctgggataag
     7681 cagattcgaa gcattaaatc cctgaccaaa taagccacca ctcccaatgg caatctgccc
     7741 ttgagcctgc tggtaagtcg ttgtttgggc aaactcaaag ggattgagcc aagccaaaat
     7801 ccgattaatt tggtaggtcg gcattccaat ctggtgaaga aaagctcgtc cgtccttgct
     7861 aataaagata gctaagaaac cagcaactcc tgttacagca gtcacaaata ctgggataat
     7921 aattttccaa gaaacccctg ataataaaac gattcctgag aaaatggcta caaaaaccaa
     7981 agccgtcccc aagtcacttt gaagtgctaa aagaactagg actggaatgg taaagagaat
     8041 catccagaaa attaacaaaa agtccagcgg aaccgtgcgt ctccattcct tatgtttctt
     8101 tgtaaattgg acaatgacac gagccaacat gaggatatag gatatcttca taaattctga
     8161 cggttgaaat agggtaattc catttattga tacccagttt ttggcacccg ttgatgcaac
     8221 taagcttgga ttataaaata caatcggcaa gatcataagt cccaagccta aaatatatag
     8281 aaagggggtc accttccaaa gaaattctgt attaaagagc atgaccacaa aaccaatcac
     8341 aagccccaag gcaatccagg cgacctgctg ccctaaaatg ggcagaatat tattgggata
     8401 atcatgacta acggctatat agatagccac cacaccgatg accagtagaa aaaatactgg
     8461 caagagcaaa ctgtaatcga ctcttgagtc gagagaacgt ttcatatata ctaaccttat
     8521 actttcatac aatactattt atcaaagttc attaaaaaat ctatcaatag cctcgtcaac
     8581 ttcggatcga gagatggttt taacagtcgc ttcttctgct agagatgcta ctatttgttt
     8641 gccgtatcgt tttccgacga ttctcctatc caaaataaga gttaaggaac gttggtattc
     8701 acgtctcata cttcttccca aagcctgttt taaacgaata atggccattg gcaattgata
     8761 atcataaaag gcatttttcc cttcttgatt cagttcttga ttaatctttt tcgtcaaggg
     8821 ttcttgagga ttttggaaag gaagcctcgg tacaacttga atcacaaaag gatggcttga
     8881 aaaatcaact ccctcccaga aacttgctgc accaagcaag atttgttgtt cacctttttc
     8941 aaagcgtttc tttagctgat gaacatcccc atttttatac tgggccaagt gactaactgt
     9001 aagtaaatcc gatactgcta gaagcatgtc tttagcggta aagagaacca aaatcggttg
     9061 ctggaaagct tgaatttcca ctagtaaagc agctacctct ctggcataga cttctaagga
     9121 ggtttctgtt accagaggga aatctttgac catgaccact tcttgttcct gttttccccg
     9181 agattcaatc ttgacaaatt tagcttcagg atagcctaaa aggtctgcca aagaaaccct
     9241 ctgactaatc tcaagagtag ccgatactcc caggacttgg caactctcag gaagtaaaga
     9301 ggacagaaga gtacggcctg attttgtaga agaaatctga attttctttt gactcgtttc
     9361 agttacttca agccagtaat caccttcagc tgtaaaatag cgaaccagct catcaaagcc
     9421 ttctacttct aattctgaaa aatactgatg gagattgtcc agagaatcta agatattttt
     9481 cctagattta ccagactgaa attgttctat caagtagaga cactcaaagc gaatactttc
     9541 tagtatccgt tgttgaaccc tgttttcttc tcctactaaa gccttatcaa ttaaatcgat
     9601 aatagattgt atatcgtagg tctcttgaag cagattttct agagctaaca aaatcttttg
     9661 gacttcatca ataatcagta aacggtcact gacaaattca ggattatctt caagtctggt
     9721 cacgagatag gcatgattag tcactaaaag cttgcaagtc tctgcccttt cttgactacg
     9781 tttccaaaaa tcttccgtca caaataagct ctgggatgat aaattcccat catgacgaag
     9841 gtctgctaga aaatgttggt aacggtagag ttgcccgatt tcatccaaat ctcctgtctc
     9901 tgtctcagta agccagacca agacttgcat tttaaagcgt ctaaataagc gattttcatc
     9961 attttcctgc aaggaatgat aaaaggcatc caacttcaga taattttgtg gtccctttaa
    10021 gctatgaata tctgtatgga acacttcctt gagacgttta ccttcttctt ccatgatttg
    10081 attttgaaga atctttgtcg gaacactaag aacaatttgt cgctctttgg attgagataa
    10141 agcgggtaag agatagccat aggttttccc aatccctgtc ggcgcttgaa tcagaaagac
    10201 aggttcatct ttcaatagca agccaacctc tttagcaaaa ctttcttgtt cctccctcac
    10261 ttcaaggttc aacagagaaa tatttttaga aaagtcttga gatagttttc gtagctccag
    10321 agaagctgcc gttttcttaa aatacagacc ttgaacttgg accaagtctg gagaactcag
    10381 gatagattgg ttgcgataag tttcctcaat aaccaggtag gactcatata ggagagcgtc
    10441 agccatttcc agcaagcgtt ccaagagacc tttaggaagc tgggtcatct tttcccgtaa
    10501 aaaaagaagt aattctgctg tagcttgggc atctgaaagg gctgtgtgtg cgtgtttaag
    10561 aggaattcct aattctcgac acaaaatcgg caagctatat ttttccagtt cagggaaaaa
    10621 gacctgggcc aattcgaccg tatcaacacg agggtttctt agctcatagc cttcaaaaaa
    10681 taaattttcc gccaagagat tagcatcaaa ctgaacatta tgggctacaa aaatcccatc
    10741 ctccaccaag tcaaatattt ttctggcaac ttgcgaaaaa tcaggtgctt gcgccagacg
    10801 ttggtctgtc aatcctgtca gttctttgat atgagcatcc aagggttcat gtggattgac
    10861 atccgtcgta tagtgatcga cgatttctcc gtcctcaatc acgacaattc ccacttggat
    10921 aattttagcc ttactacctg tgctagttgc ctctaaatcc accacaacat agcgattacc
    10981 aatcgttttc attataaaaa attataccaa aaaaagggcc atttggcacc tgcaaacata
    11041 ggataatttt caaactcaag aacactactt ttcttaaaaa tcccatcgaa tagagcattc
    11101 tagcctgctg atttttgtaa aatagtgaag aaatacgaaa aattgagaga aaaaatgttt
    11161 gcatcttcct tctctcaacg aatcatgtat tttatctaac catgtcagga tcgtagtcat
    11221 aaaatcctgt atcaaggaaa cggtttttag ggtgaagttt acgaacttct tcatccagca
    11281 agaagacttg atcatggcta aagttgccct cttgaatcaa gttacgcgct tggataaaga
    11341 gttttgcaat ctcaacaaag ttctgaccag gagtgtagag cccttctagt ttccagcgag
    11401 taaagccatg ctccaccaat tctgttaatt tgatcatcaa atcaaggtca ttgttggcaa
    11461 agatatgggt cccatgatta tcttcaaaaa tggaatagtg gctctctgga tcacttggct
    11521 cagccaagaa gaggtcacgt ttatgcgtct tttcatcatc gatatgtgta aagttatagt
    11581 agttttgcaa gagtggacgt ttagaatgat ggatgacgct agcaccgtaa accaaaactt
    11641 cagcaggaat ttccaaaatc tctggcattt tgaaaagttc agctgatgga atttcacgcg
    11701 ccaaaacagc ctcagatgcg ccagcctttt gtccccagaa gttaatctga cgactgctag
    11761 ttaccatggt tgaagcatcg tagatggtct taaatgaata accatcgcgg ttaactacgt
    11821 aaaagacgcc tgcatcccca atcgtaatat agtctgtctt gatttcttcc aagaagtcta
    11881 agaaaggctt gatacggtcc atcatatctt ggtgcatgag agcattgacc gcaacgagca
    11941 attccttacc agcatcatga accaacttag cgatttcacg taattggtca taactaaagg
    12001 tcgttggcag acgaaggcca aaatctttct caccgacata gatacggtct acgccagctt
    12061 cgagtagttg ttcaacttgt tcaatacttt cagcagttgc tgtaatgaca atcttttcca
    12121 taagaaaaat tataccacat ttctccaaaa tcagccatgt tttgaaaatg aaaagggact
    12181 ttattctttt tgtaaccttt ctgtaataat tctctgccga aaaaatgata aaataggtat
    12241 gtactgttaa ggagagaatc atgcccgtaa gaaaattaca atcctatgag gtagactatc
    12301 aagaagaatt aaaccagcag cttcctcatt accaagctta tacacctgaa gcgcaatctg
    12361 atgccaatct caaggaaatc ctatttttta ttaatatcgc tgttttttgt atctgtattg
    12421 ctatctttag ttttatcttt ttagcattaa aattatcaac tgctcttgcc tttgccgcag
    12481 caatcggatt cagcttactt gttttaaaag ttcaacggtc tattatcaaa cgaaaacgta
    12541 gaagataaat ccaaaatatc gccccaattg gagcgatatt tttatttttt ctttttagat
    12601 ggtgtctgaa tggcaatctt cacttcaaag attgttccct tgggtttatt atctttgaca
    12661 gtaacagttc cttttagagc atctacaatt tgcttggcta gggataatcc taatccaaaa
    12721 ccaccttttt gccgggttct agccttgtct actcgataaa aacggtcaaa aattttcttt
    12781 ttatcttctg tcgaaatacc gattccatta tcagaaacaa gtaaataaag attgcgatcg
    12841 gtcgccgaga taagaaaatc aatttcacca tcctcctcag tatacttgac ggcattatcg
    12901 aaaagaatgg tcatcagttg tttcagaaga agctgatctg tgacaattgt tcgatggata
    12961 cgattttcaa aacggaagac acgattattt tccgaagcaa tcatctcgta gtttgtgaaa
    13021 gttgtattaa aaaagctagt tggaacttct gcaagctccg gcttaatccc atcatctctc
    13081 cgagctaagt tcagcaagct tgtcgttaaa aaacgcatat ttcggacttc ttccaaactc
    13141 gatgcaatgc tttcgctcac atccataatg gtagcttctg gcttacgaaa aagggtctct
    13201 aagcgatttt gcaaaactgc gagtggagtt cgtaactcat gactggcatt ttccacaaaa
    13261 gactgttgct tctgcatact ctcaagcagg ggcctaacac tgaccctagc tagatagaga
    13321 ctggcaagta aagacaaaat ccagaaacta gccatcacga ccacaatcaa ttgctcatgc
    13381 ttttgactag cctgctccaa ctgactggta ttaatcaaga cagcagcata ctttatattg
    13441 gttgaaaccg aactaatatt ggtctccatc aaaatcacac gatagatttc ttcctgccca
    13501 tagctattaa aaacctgaat ctggtagata tgtcctagtt ctttcttttc taacttaatc
    13561 ttatccaagc ccaaaaatcg atttccagaa agaagttgag taaagtcttt atcaaagaga
    13621 atgacttccg tattggaact gacattaggt tttatttcta ctttactagc gtccgcccta
    13681 gcattttcta aatctttaat ctcttctgtt gccctattta tagccagctg aataactgct
    13741 tgaggatttt cactcagtcc atgaagctta tcgtccaccg aagtataaag actcgaatgc
    13801 atgacttgta aaataatcag agtcattgta gaaaaaatca gggtgaagac accgaagttg
    13861 cggataaaat aactaaagtc atccgcatac catgtttttt taagtttact gaacatcttt
    13921 taaaagatac ccaacactac gcaaggtttg caaattctct gcaaaagtgg ttccctttaa
    13981 tttcttacgg acttttgaaa catagacttc gacaaccgaa atcgttgtat cactatcaaa
    14041 tccccataga cggtcaaaaa tctgcgtctt aggcaaaatc acattttgat tttgaaggaa
    14101 ataaactagt aaatcgaact ctttccccag caattcgaca ggagtatctt caactttaac
    14161 ggtattggtt gataaattaa ccacgatatt cccataagtc aaggtgtttt cattaaactt
    14221 ccctgaacgt ttgagaaggg cctgaatccg cattttaagt tcttctaggt agaaaggttt
    14281 ggtcagataa tcatccgctc ccagttcaaa tccatgtccc ttgtcatcca aactttcctt
    14341 ggcagtcata atcagaactg gtgtcgtaat tcccttttca cgcaattctt ttaagacttg
    14401 gaaaccattt ttttctggca acatcaaatc cagcaaaatc aagtcataga caccactctc
    14461 agcttcgtag agaccttctt ctccatcaaa tacctgcata acatccgcaa aatcgtctaa
    14521 aaagtcaaat actgaatttg acagacctag gtcatcctca accaataaga tttttatcat
    14581 gagaaactcc tccttattaa aactattata ccaaatttgc cttaaaaaaa actcaactct
    14641 ctgcatttta catgagatag ctgagttttc tttttatttt aggcttattt atgcatttcc
    14701 gtattgaaga acaactgctt cgactgcagc tttttcacgg ctaatcaagt caacacgcgc
    14761 tgcaatttcc ttgattccca taccgatgtt acggctaaga gcaaggtcag aaagttgcgg
    14821 ttcaaagaac tccttgtatt ccgccaagcg ttgctgagtc ttaaatacat gagcaggaag
    14881 gataacaaag ctatcaaagc tcatatctcc tccaagggct gccttaatcc aagcccagtt
    14941 ttcacgcgcc caagaccaag ctgttttctg agttgcttga tgagctagga attggtaata
    15001 ccaagcagac aagtcctgtg gtttgaccac aaatttgtcc ttccaagaag taatcaagtt
    15061 ttggatatta tccgcatctg tactgtatgc aagagctgct gccaactggc gtttaaagac
    15121 agcatctgtt gcgtgagtat aagtatcaag ataaagtgct aacaagtctt tagtctcatg
    15181 atgtttcatc tcattaatca gaacttgtga gcgaatagct gctgggagtc ctgcaagatt
    15241 ctccttgtgt gttgcgaaga tttggctagc gacttgacta gcttctgcat catttgagcg
    15301 aatcatcatc gaaacagcca gctgacgaac caattcatcc tcatctgatt ctccgtcttt
    15361 agcttcaaaa ccaagacggt catagttatg acgagccaat ttagcaacca gtcctttgaa
    15421 ggctgtttca gcatccgttc cttcatcaat aaagcgctca aaggctgaaa tcacttgaga
    15481 aacagctgaa accaccagat aagactcttc cttagcaagt ttatcaagaa ctggaagcaa
    15541 gtctgcataa gaaatgtgcc ctgcctcagc caacaaacga cgttcttgaa caatttgcag
    15601 tttgcttgtg ttatcaagtg tctctagctc agcaagaaca gcttctaaca agtctccttg
    15661 atagtcggta atatagtggg cagtattttc agtgttgaga cgaagagctc cttcattttc
    15721 agcaagaaga gctgcgtagc cagggatttc gatactttca gtttcgagtg tatcaggcaa
    15781 gcctttccag ttgctattga ggggcaccac ccagagacgg ttcttgtctt cgttctcacc
    15841 gatgaagaat tgtttttgtg aaatcttcaa gacatcattt tcaactttaa cagtaagaac
    15901 tgggtaacca ggctgttcca accaagaatc catgaaggct gcgacatcac gtcctgacgc
    15961 ttgaccaagg gcatcccaaa ggtcactacc aatggtgttg ctgtattggt gtttttcaaa
    16021 gtaggcgtgc aaacctttag caaaatcagc atctcctagc caacggcgaa gcatatgcat
    16081 gagacggctt cctttggcat agacgatagc gccgtcaaag agtgtattga tttcatctgg
    16141 atgtttaact tcgacgtgga cagactgaac gccatcagta gcgtcacgtt caagagcaag
    16201 aggtactcca cctgtttgga aatcttcaaa gatattccag cttggttcga tggtatccac
    16261 acagacgtat tccatcatat tagcgaaact ttcattgagc caaaggtcat cccaccattt
    16321 catagtcacg aggttcccaa accattggtg agccaattca tgggccacaa caagggcaac
    16381 ttgttgacgg ctagcaaatg tagagttctc atcgacaacc aagtaaactt cacggtaggt
    16441 cacaagaccc cagttttcca tagcaccagc tgagaagtca ggaagggcga tgtggagaga
    16501 ttgaggaatt ggatacttaa ctccatagta atcttcgtaa aactcgatag agcgaacagc
    16561 gatatccagt gagaaatcaa gatttgaaag tggatgtgct ttggttgagt agacacctac
    16621 cagggtacca tttttagttt tagcggtcac cccttgcaaa tcaccagcaa caaaggccaa
    16681 caagtaagaa gacatgcgag gtgttgtctc aaacttccag atacctgttt ccttacggtt
    16741 ttcaacatcg atttctggca tgtttgacaa ggccaattca ccttctgctt ggtcaaagcg
    16801 aagagagagg tcaaaagttg ctttggcttc aggctcatcc acacatggga aagcttcgcg
    16861 cgcaaaatgg ctctcgaact gagtagacaa gacctccttc ttgactccat caactgtata
    16921 ataagaaggg taaatccctg tcatgttgtc tgtaatttta ccagaaaagg caagaaccaa
    16981 ttcaacttga ccagcctcag ccaattcgat atgaagggct tcattgtcat ggtcaactgt
    17041 aaatggacga gcttgacctg caacttctac agaggtgatt tccaagtctt tttggtggag
    17101 ggagatgcgg tcactctgtg cttgaccagt gatggtcact ttcccagaaa aagtcttggt
    17161 ctcacgactc aaatctaaaa ataaatcata atgttcagga acaaattgct taataaaatg
    17221 ttcaactgct tgcatagttt tctcctattc taagtttaag agctgctgct cttcttcaaa
    17281 caaacttatt atatcatatt ttgcttgaga aaaaaggatt ggacagcgat ttccaaaact
    17341 ttcttccttc taatagtcta cagtgggtag accttgcgaa attcttctaa aacaaccttg
    17401 ctttcaggtg taaaagtcaa tcctgcttca cccaagcagt ctctgaaaaa atcctcatga
    17461 acctggcgcc aatagacaag tgtcttgttt ccctcacctt ccttataggc atggtcagct
    17521 gacactcgat tgaagggttt aacagaaacc tttgtaattt cgacaatgca gacagcctga
    17581 ttttgactat ctaaaatgac atcgaaggtc cctacttggg gaagtggttc gtcttctagt
    17641 acatagaggt cataggctga tgctgttgct gtcttttctc ctttaaacac caaatccgct
    17701 aaaaggtctg gttcaactcc aaaagcccag gcatcgattt catctccgat agaaggattg
    17761 atttgtttgt atttattcca catttcttgc ggtatcatgg gtactccttt gtaatttttt
    17821 actttcttct tttatgtgtt taagatgatc tggatggtca atctctaaat caaaaatctc
    17881 tggaatagaa ctgtagtgga taatgcactt gatacccaac tgattcattt tttgtatgaa
    17941 agaagtattc agatagcctg ctacagcaaa attaatcttg ttctttcttg ctttatcctg
    18001 catatctctt agcatatcta acattattgg actttccata tcatgccatt gactgtttct
    18061 catagtcgca aaaacaaagg aagtcaaatc attcattcca actacaatct ttgaaatgcc
    18121 cgtttccagt atactagata agtcaaaata cgctgacggt aattcaatca tcgttccgac
    18181 tttcccagta aagccatact gacgcaatac tgtaatagct tgttttaatt ggtcggcatc
    18241 attgacaaaa ggaaagataa cagatagatt gggggtggtt tgataaactt ctgtaacgac
    18301 atgtgcttca gcctgaaatt catccaaaca cgccagtaaa cgcctagttc ctctatagcc
    18361 aaacaaggga tgcccttcgt caaaaaactc tttagtcccc actaaacaat tggcttctgt
    18421 attcgttaat tcagtaaaac gataccaaac ttcttcatct gagtacaggg agcagatagt
    18481 ctctagataa tcttttacaa attgctgaca acttgctaat atgatgtttt gattaagctc
    18541 tctcaacaaa tattccccac gaatcatgcc gacgtggtga aatagttgag gataaatttt
    18601 ttcaagaatt ttttcgccac taagggcaag ttgatttctc atcattcacc ttccaattca
    18661 tgtaagaagt cttgtccagt tctggaaatc ctaataattc agacttaacc ttcaagacta
    18721 atggcgatgc attttcttct gtaatctctt gaatatccat ccaaatatat ccaagtgaat
    18781 cattcgcacc atcagacaca gcttccgaaa tcgtaacttg aggtgcactc tcattcattt
    18841 caacatcata caaggccatg acatggtgaa ccataaaatt ttttaactct tctctgacga
    18901 aaacatcgta gattcgagga ttagagtagc ttctaacagt aaatcccgtc tcttccataa
    18961 cttctctagt cagcgtttcc gtcagtcctt caccaagttg ctgactgcct ccaggtagat
    19021 cataccgatg ttgataaggg cctctcgttt tttcaatgca aagtaacttt ccattttcaa
    19081 agcaaacaca gtagacccca aagtgatttt tgatttccat ccaactcctc ctacttcaaa
    19141 gaccagccac catctattgt caagatttgt ccttgcatgg cgctcgcttt tccacttgct
    19201 aaaaaaagac taagctctgc tatttcctct ggttcaatcc agcgcttgat tggtgtttca
    19261 ctagccaccc agtcagctaa gccacttggt tcaaaatccg cagcggtcat agctgtcttg
    19321 actgctcctg gagcgatacc aaagacctga atcccagctt cagcatagtc tagagccaac
    19381 tgcttggtga agccagccaa ggcatgcttg gatgaagtat aggcgtgacc acctccacct
    19441 gctaggctag aagcaatgga acacatattg atgatgattc cctttttatt ttccagcatt
    19501 tgtgtcaaat aataccgagt caactctact ggaataatgt agttgatttc aaaaatctct
    19561 tgaatgtcct gcgccgtttg ttccaacagt ggtttgtaat catccaaaac tccagcagta
    19621 ttacacaaaa catccacctg agggcaccag tcaaaaatag gttccaagtc caaggtcaaa
    19681 tctctctgta aaaagtgaaa atcaccctct aagagtggct tttcaccttg gtcaactcca
    19741 taaacttgat agcccttctc taaaaagagg cgagcttgag ccaatccgat ccctgaactc
    19801 actcctgtaa tgagtacacg tttagtcatg cacttctacc caatccgttg ccaaaacatc
    19861 acaaactgtc gggctccaca tggaaaaacc ttctccttcg ccagaaacgt tgattaggaa
    19921 ataaggtgtc atttcaagtg caagcccatt ttgctcgatg gtatcaaaga gttggacata
    19981 gttttccgca cctccccaac cagttcgtac atattttctc ttagccttta acccaggcag
    20041 gatctcttca aatgtcatgt ttttctcctt taattctaca ttcttcattt aattatagca
    20101 aaaaaccgct ttatacggct ttttgaatgt gagttattca aacctgctac tacttacggc
    20161 aaattattcc ctgcagcaag ataaatttca taccattctt ttcttgttaa gctaaagttt
    20221 gccgctcggc taacttctct caagtgctta ggatttgttg tacctacgac tgcctgcatt
    20281 tttgctggat aacgcaatat ccaagaaatg gcaatagttg aagaggttac tccatattta
    20341 atagctaaac gatcaagtac ttgatttaaa gcttgaaatt tctcatttcc aacaaaattc
    20401 cctttaaaat acccgaattg taagacagac catgcttgaa tgaccacatc gtgtaattgg
    20461 caatattcaa aaatgctgcc atctcgcata gctgcttgac tatcttccat attaacatga
    20521 aaagctgatt caaatcctgg agtaaaagcc gcactcaatt gtagctgatt aacagctaac
    20581 ggctgcttga catctttttt aagcaactcc atcatcatag gattttgatt agaaactcca
    20641 aaatctcgaa ctttaccttg tttataaagg agattaaagg cttctgctac ttggtcagat
    20701 tccatcaaag catctggtcg atgaaggagc aagctatcta gatgatcaat cttcaatctt
    20761 tgcaaaatac cgtctactga ttttataata tagtccttag aaaaatcaaa ataggtaaat
    20821 tcttcaatgc gaatgccaca tttggactga atccacatct tttctcttaa atctggacga
    20881 ttttttagga caagacctaa cagttcttca caacgaccac gaccataaat atcagccaag
    20941 tcgaaggcat tgattccaac agaaagtgct gtttctacaa gctcttcaac ttcttttaca
    21001 gacttatctt ttattctcat cattccgaga acaatttctg ataattcttt gtcatcttga
    21061 ccaagagtta tgtatctcat caaatttttc tcctttaatt tctaacattc ttcccttcat
    21121 tataacaaaa aaccgctttg caacggcttt ttgactatac ttcactccat tttatcttct
    21181 taaacccacg gaacaagaca aagattccaa taaagaggac agctaaagga ataacttttg
    21241 taaggaaaac atttgaaatt cccatccact cataataacg gagcagagaa cccaccacaa
    21301 gatgggcaat aatcatactg acaaatggac gaaagaccgt ttctttccaa ttccaaatac
    21361 cgataactag cgaaatcgta aagacagaca aactatccca gggagccgga atataaaagg
    21421 ctccttcttg tatgaagctt gccattccta catatcctaa aataactaga agaactatag
    21481 tcccaacaac aatgtaagtg ccaattttca ttttaggaga atcttggact aaacttcttc
    21541 gtaaaattgt ggccacaagt ccaaatccaa tcagaaaaat aagaagttgc cctaaaaatg
    21601 tgagcaaatt gactgttaag agaggacctt tagaaaaatc acttagtagt tgataataac
    21661 gtaataccgc caggacaaga attggcgtca aaagggactc tttgatagaa ctgcgaggtg
    21721 ctcccttgag aatctctttc attatttttt taggattctt acctagataa tcctctgcac
    21781 tcatgccatc tcgttctgct tctgagaaat ctagcatcat caaatagatc tgctctctga
    21841 gatagtcttc atcatagaga aatccagcaa gattaaaact ttcccacaac tcctcaaaat
    21901 acttttgatt ctcctcagaa aactcatgta gcaaagcgct tgtttcttcg taatacttca
    21961 ttttcttcat ggtttaaccc ccattcttaa tcccttctac tttttgactc aaatcgtccc
    22021 attgttgcca aaagactgag acacgctctt ctccttcttt cattaatgaa aaatacttcc
    22081 gatctggacc atctggcgac gggcgcatgt cgcctcttat ccattgattt ttttctaact
    22141 tttgcaacaa aggataaata gttcctggaa cgatagtatc aaatccagcc tctcgcaaag
    22201 tctgaaccaa ctcataacca taccgctctt tttgaccaat catatccaag acacaacctt
    22261 caagaacacc ttttaatagc tgagtttctt tcatcacttc tcccttctaa tctattttgt
    22321 aatacctact agtaacttca cctatagtat atcacttcta cactagtttg taaagcataa
    22381 tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccctac cgtatgtatg
    22441 gttactgact tcgtcagttt catctacaac ctcaaaacag tgttttgagc tgacttcgtc
    22501 agtttcatct acaacctcaa aaccatgttt tgagcaacct gcggctagct tcctagtttg
    22561 ctctttgatt ttcattgagt ataaataaaa aaacagaact agtctgaact agtcctgtct
    22621 acttttaccc aatcacactt ccatttggta cagctggatc aactgtgaga agggttaatt
    22681 tgccatcatg ttcagctgag agaatcatac cctggctgac atattttttc atcattttac
    22741 gtggtttgag gttagcaacg atttgaactt tcttgccgac caattcttgt tcatttggat
    22801 agtattttgc aattcctgaa agaatctgac gatcttctcc atcaccagca tccaagcgga
    22861 attgaagcaa cttatctgaa ccttctactt tagacacttc tttgacttct gcgacacgga
    22921 tttcaacctt gtcaaagtct tcaaacttga tttcatcctt gtttagtttg agctcaactt
    22981 cgtccggatt ccattctttt tcgactgctg gtttattgcc ttccatttgt tccttgatat
    23041 aggcgatttc ttcttccata tttagacgtg gaaagatagg tgttcctttg gcaactacag
    23101 tcacatctgc tgggaagtca gccaaactca agttttcaag actagaaact tcttccaaac
    23161 caagttgagt caaaactgca cgactagttt ccatcataaa tggttcaatc aagtgagcaa
    23221 ctacacgaat gctggctgcc aagtggctca tgacacttgc caattggtca cgaagagctt
    23281 catccttggc caagacccat ggtgcggtct catcgatgta tttattggta cgagagatca
    23341 gagtccagac tgcttcaagc gcacgtggat agtcaactgc ttccatgtgt gtatggaagt
    23401 ctgcgattga ttgttctgca acctcagcaa gaacatggtc aaattcagtc acaccttcta
    23461 cataggcagg gatttgtcca tcaaagtact tattaatcat ggaaaccgta cggttaagga
    23521 ggttcccaag gtcattagcc aattcatagt tgatacggcc gacatagtct tcaggagtaa
    23581 aggttccgtc tgaaccaact ggaaggttac gcatgaggta gtaacgaagt ggatctagtc
    23641 cataacgctc taccaacatt tcagggtaaa cgacattccc ttttgactta gacatttttc
    23701 cgtctttcat gacaaaccaa ccatgggcaa tcaaacgatc aggtaattta acatccaaca
    23761 tcataagaag gattggccag tagatagagt ggaagcgaag gatatctttt cctaccatat
    23821 ggaagactgt tccattccag aacttgtcaa agttaccatg ttcgtcttga gcgtagccaa
    23881 gagctgtcgc atagttaaga agggcatcaa tccaaacgta gacaacgtgt tttggatttg
    23941 atgggacagg cactccccat gtaaaggttg tacgagatac cgccaaatct tccaagcctg
    24001 gctcgatgaa gttgcgtagc atttcattaa ggcgaccatc tggcgtgata aattcaggat
    24061 gagctttgaa aaattcgacc aaacggtctt ggtatttgct aaggcgaagg aagtatgatt
    24121 cttcagaaac ccattcaacc tcatgacctg atggagcaat accaccagtc acatttccag
    24181 cttcatcacg gaaaacttct gccagctggc tttctgtaaa gaattcttcg tctgatactg
    24241 aataccaacc agagtattca cccaagtaga tatcatcttg agcaagtaag cgttcaaaga
    24301 cctgtgcgac aactttttca tggtagtcat cagttgtacg gataaattta tcgtatgaga
    24361 tatctagtaa ttgccagagt tctttaactc caaccgccat tccatcaaca taggcttgag
    24421 gtgtaatacc agcttcttcc gctttctgct ggattttctg accatgttca tcaagacctg
    24481 tcagataaaa tacatcgtag cccatcaggc gtttgtaacg tgctaggaca tcacatgcga
    24541 tagttgtgta ggcagaaccg atatgaagtt tcccagatgg atagtaaatc ggcgttgtaa
    24601 tataaaaatt tttttcagac ataatttttc ctttccaggc aaatgaaacc tgtttttcta
    24661 acacttcatt atatcacatt tttaatgaat ttcgataggg aaatccatac aaaaacaaga
    24721 tagacgagtg tccatcttgt tgatctcatt cataacgaag ggcttcaatt ggatcaagtt
    24781 tcgatgcctt gttggctggc aagactccaa aaatcatacc aacactagcc gaaactgcaa
    24841 gactaaatag ggcaactggg attgatactc caacttctat accttctatt aaaccttgca
    24901 gtaacaaacc tgctaaggca gttaaaccac ttgcaattgt caagccaatt aagccaccta
    24961 acaaggtcaa aatcatggat tcaatcaaaa actgaattaa aatattggca cgtgttgcgc
    25021 ccaaagcctt acgaagacca atctcacgag tgcgctctgt caccgaaacc agcatgatgt
    25081 tcatgacacc agttcctcca acaaagagag aaatccctgc gatggaacta ataatcgtcg
    25141 tcataaaact aaacgattgt tgaatttctg caaatacaac ggactcatct gccacctggt
    25201 attctccctg ttgtaagcct gcaagctctg tcatttttcg tgccagttct ggacccagag
    25261 ttggggttaa actggtatca ttcactcgaa agacaatact agctatttca tctatattaa
    25321 aattcgcagc aagagagata ttggtagtaa taggcaagcc accaaactca tatatttttg
    25381 accttttagc ctccggacta gtataaaccc caatgacccg gtaactaaat ccattgactt
    25441 ctacaacctt gttaatagcc tcttgaggag attcaaataa actaatggac aattcctcat
    25501 ctagcaaaat gacacttgca aactctttga aatcttgctc tctcagacta cgacctgcaa
    25561 taatttcatt cttaacagcg tccatgtaag ttctgtttcc acctgtcaaa ttagcattct
    25621 caaccttttt atcttgatag gtcaagatgg cattcgttga attggttaca tagtaactat
    25681 ccactccctt cagtttagct gcctcttgga cccaggattc ttgcggtttt ggcggttcaa
    25741 caggaacttc ctcttccttt ccagaaaccg taaaagctga ttgtttctga gtaaaagacc
    25801 catctttact ttttttagga gagaaaaaga cgctaatatt tttctgagat ttagtcatat
    25861 ctttattgac ttgacgagat agggaatcac ccaaagccat aatcacaaca actgatgaaa
    25921 caccgataat aatcccaatc atagtaagca aagaacgcat cttgtgagcc atgatagatg
    25981 agaaggcaaa tttcagattc tgcatcttag ttttcctcct ttcctaactg agcactgtca
    26041 gacgaaatga ccccatcccg aatgacaatc tgacgtttgg cataggcagc aatctcaggc
    26101 tcatgcgtta ccatgataat ggtttttcct tctttattca aatcaaccaa taattgcata
    26161 atttggttac ctgttttggt atccaaggct cctgtcggtt catccgctag gataatagaa
    26221 ggattgttta ccaaggcacg cgcaatggct acacgttgct tttgaccacc agataattct
    26281 gaaggtaaat ggtgactacg ttctgtcaat tcaaccttgt ctaaatattc ctcagccaac
    26341 ttgcgacgtt ttgaagacga aactcctgcg taaatcaagg gcaattctac attttgcaga
    26401 gcattgagct tcgatagaag aaagaactgc tgaaagacaa aaccgatttg ttggttacgg
    26461 accttagcca gttgtttttc accaagccca gccacttctt gaccttcaag ataatattct
    26521 ccactggttg gtgtatccaa catgccaatc gtattcatca gagtggactt accagaccca
    26581 gatggtccca tgatggctac aaattcaccc tcattcactt ctagattgat atttttgaga
    26641 acctgcagtt cttggtcacc attacggtag cttctgaaga tattttttag actaattagt
    26701 tgcttcatca gccttcacct cttttccttc ttccaaggaa gatgttggat tactgatgac
    26761 cttagcacca tttgtcaaac cagaagtgat ttcttgattt tctgcgtcag catttcccaa
    26821 tgaaacctca acttttttag ccttttgttg ttcatccaca atccagacat aatttttact
    26881 atcatccatt actagactgc taacaggaac aagaatagcc ttagttttgc ttttaacctc
    26941 aatgttgaca gaaaaacctt gtttcaaatc accaacctcg cctgtcacat caatagtata
    27001 agggtattta gaacctgtat tattcccggc tgctggacta gctgcttcac cattgttttt
    27061 aggatagtca gaaatatagc ttaatttccc agtccatttt ttatcaggat acactttaga
    27121 agtaaagctt acttcttgac ctacagaaag gttggctaga ttgtactcag acaattctcc
    27181 cttgacttgt aaattttcat tgctgacaat atgaaccata acttgactcg cccctgttgg
    27241 agatttagaa acattgctat tgacttcgac cacagttccc tctagggtac tgagaacagt
    27301 tgttgcatcc aattgacttt gagccttgct taattgcgcc gcagcatctg cacgcgcatc
    27361 acgggcatca cccaattgag cgtcaataga agcaacagaa tttccagcca ctggagttgg
    27421 gctttgcacc gttgcatctt ctcctcctac tggcgctggt aactgtggag ccggagctga
    27481 agcggcttca tttcgtgctt gattgagttc attgatatga cgatctgccc tagctactgc
    27541 tcgactagct gaatcatagg ccgcctgcgc ttctgaacta ctgtacttga ctaaagcctg
    27601 cccttcgctg accttatcgc ccacagaaac aaggatttca tctaaatcac ccttactagc
    27661 atcaaaataa acatattgtt cattttttgc tgttactgtc cctgacaata aaacagagga
    27721 tgccacgctt ccttccttgg caacaacaag atgagtaggc tcatctttta gagcagtctg
    27781 agaaggttgt ctaaagagta aaatcccccc agcacccaat acaactacac tcgcagcacc
    27841 gattgctgca tacagttgcc actttttagc tttaccattc tttttcttca taatgaaact
    27901 ccttttcttt tttacaatac tttgctatta taccaaattt ccctccagca aacaatacag
    27961 ttcaggatta aacaatcgtt cggaattttg cttttctatt gtactagata tataatacac
    28021 tatcttgact tatttggcaa gcttttttgc aattttttgt gcgtagcaga tttttgcaat
    28081 caaatcaaaa aaagaataaa atataactat caaaaaagac gctctactat ttaaaagatt
    28141 acaaaggagt tttcaatgag agaatatgat atcattgcta tcggtggagg tagcggagga
    28201 atcgctacca tgaaccgtgc tggtgaacat ggagcccaag cagccgttat tgaggaaaag
    28261 aaattaggtg gaacctgtgt caacgtcggt tgtgttccta aaaaaatcat gtggtacggg
    28321 gcacaaatcg ctgagacttt ccatcaattt ggagaagact acggctttaa gactactgat
    28381 cttaactttg actttgcaac cctacgtcgc aatcgtgaat cctacattga tcgcgctcgt
    28441 tcttcttatg atggcagttt taaacgcaac ggtgtagact tgattgaagg tcatgctgaa
    28501 tttgtagatt ctcatactgt aagcgtaaat ggtgaactga ttcgtgctaa acatatcgtg
    28561 attgctacag gtgcccatcc aagtattcct aatattcctg gtgctgagct aggtggctct
    28621 tctgatgatg tatttgcctg ggaagaactt ccagagtcag ttgccattct aggcgctggt
    28681 tatatcgccg ttgaattagc tggcgtactc cacacttttg gtgtcaagac agatctcttt
    28741 gttcgccgcg atcgtccttt acgtggtttt gattcctaca tcgttgaagg tttggtcaag
    28801 gaaatggaaa gaacaaactt accacttcac actcacaaag tccctgtcaa gttagaaaaa
    28861 actactgacg gcattaccat tcatttcgaa gatggtacta gtcacacagc tagccaagtt
    28921 atctgggcta caggccgccg tccaaacgtt aagggcttgc aacttgaaaa agctggagtg
    28981 actctgaacg aacgtggctt tatccaagtg gatgaatacc aaaatactgt tgttgaggga
    29041 atctatgctc taggtgatgt aacgggcgag aaagaactga ctccagttgc aatcaaggcc
    29101 ggacgtaccc tatctgaacg tctctttaac ggaaaaacta ctgcaaaaat ggattactca
    29161 actattccaa ctgttgtctt ttcacaccct gctatcggaa ctgttggttt gacagaagag
    29221 caagctatta aagaatacgg tcaagaccaa atcaaggttt acaaatcaag ctttgcatct
    29281 atgtactctg cttgcactcg caaccgtcaa gaatcccgtt tcaaactcat aacagctggt
    29341 tcagaagaaa aagttgtcgg acttcacgga attggctacg gcgttgatga aatgattcaa
    29401 ggatttgctg ttgctatcaa aatgggagca accaaggctg actttgacgc aactgtagcg
    29461 attcacccaa cttcatctga agaatttgta accatgcgtt aatcgaaaca aaagaagctg
    29521 atataaatat gtctgcttct tttttaatga ttttcgaatc aacaaaaaaa ttatccaact
    29581 tgactagtca cgaaaatcgg ctgttacagt ttttgttacc ttgtttttaa aaaatggttg
    29641 acatcgactc ctatatgagt ataatagtta ctatacatta gaaaagaggt taactatttt
    29701 gaaaaaagct cacgtttatg ctatccctgc tattggggct gctctcattg ctgctttggc
    29761 acaaatcagt cttccaattg gacctgttcc cttcactctg caaaactttg caatcggctt
    29821 gattgctact gtctttagac cgagagaggc tgtactttct gctggactct atcttcttct
    29881 aggtgctatc ggtcttcctg tctttgcagg aggtggagct ggttttcagg ctttagttgg
    29941 ccctactgca ggctatcttt ggttttatct cgtttactct ggacttactt cctctctaac
    30001 caacagcaag agtggtgttg ttaagatttt tcttgcaaac ctcttgggtg atgcccttgt
    30061 ctttgtcggc gggattctca gcttgcattt cctagctgga atggcatttg aaaaagctct
    30121 tgctgtgggg gttcttccct ttatcattcc agaccttggc aaacttctgg ctattagttt
    30181 tattagccgt cccctacttc aacgccttaa aaatcaggct tactttacta actaaaaaag
    30241 gatatcgagt tatcatgact caatatcctt ttcttttatt ttgaaaactt atactcaatg
    30301 aaaatcaaag agcaaactag gaagctagcc gcaggctgct caaaatactg ttttgaggtt
    30361 gtggatgaaa ctgacgaagt cagtaaccat acatacggca aggcaaagct gacgcggttt
    30421 gaagagattt tcgaagagta ttagttgcct ttaaaggcat ccaccatcgt ttgaaattct
    30481 tcatttgaga gagtaatccc tttgcccatt ttagtatggt ctggactcca agcacgaata
    30541 tcaaactttg caggggcacc attaaagctc acacggttaa tttccttggt ccaacctttt
    30601 tcgttttcag aaagagttaa caagtgctct tcgatttcaa atgtaaattc tgccattttc
    30661 ttctcctttt ttagtttcat tagtttattc gtaaaatctt gtagatttta ggaaaatttt
    30721 atataatatt gatataaaag aagggaggcc aatatgagac ataaattcca gcaagttcta
    30781 aataaaatac atgatttttt aaatggatat gaccaacctg accagactga aaccaactcc
    30841 cttacagcca ctattgaaga ggctatccag aaacaaaccg ctgttcacct tatcttatct
    30901 gagacaagct ttacaggtga catcatcaaa tatgatcagc aaggccagca aattatcgtg
    30961 aaaaattttt ccaaaaatgt gagtcggatt atccgtataa gcgatattca acgcctgcga
    31021 tttgtcccct caactgtcca aacagcccaa aaaaatagat ttaagaaaga gtgagatgta
    31081 gttgcttcat cccactcttt ttcttagcga atttgttcaa aatgtaaatg aactgcgata
    31141 tgatctccat aaccacttct ttccaagtca cgttgtaaac gataggaaat atagtgttct
    31201 gcaatggtaa tgtaacctgc gcccaataaa cgatgttcaa ccatagattg aatcatactg
    31261 atagtcgcac gttccacctt ggcttcttgt aaatccaaaa ctaccttctt agtgacttga
    31321 gcaagatttt gacgcaaatc atctgtcaaa acataaacag tttgggctgc cttcaagatg
    31381 gcttggtaaa tcttatctgg attaaattca gcaatttcgc cattacgttt gattacttgc
    31441 ataggtttct cctttattct ttgttttctt tgatttctgc cagcattttt tcttcttcta
    31501 ctgtcagttg ataatgttca agtaaatccg gtctgcgctc gtaggttttc tttaaactct
    31561 cgtacaatcg ccactgacga atcttttcat ggtggccact catcaataca tctggcacga
    31621 ccatgcctcg ataatcatag ggacgtgtgt actgaggata ttctaaaaga cctgaagaaa
    31681 aactatcatc ttggtggcta gattccttgc caatcacttc tggaatcagg cgaactgtag
    31741 catcaatcat ggtcatagct gccaattctc caccagtgag gacatagtcg cctagggaaa
    31801 tctcatctgt taccaaggtc ttaatgcgct catcataacc ctcatagtgc ccacagataa
    31861 agattagctc ttcctcttga gccaaatctt cagcataagc ctgatcaaac tgctttccag
    31921 caggatcgag gagaataacg cgcggatttt tcttttcaat agcatcaaag gaatcgaaaa
    31981 taggttgtgc tctgagcaac atgccctgac cgcctccgta gggctcatca tctacatgac
    32041 gggccttttc agcattttct cgaaaattat gatactggat atccaagagc cctttttctc
    32101 gagcctttcc aacgattgag tgctccagtg gagaaaacat ctctggaaag agggttaaaa
    32161 tatcaatctt catcgtctaa cccttctaag atttctacat cgacccgttt atttggaata
    32221 tcaacattga gaaccactgg tgggatataa ggtaaaagca aatcacgttt gccttttcgt
    32281 ttgaccaccc agacatcatt agcacctggt tgcaggattt ccttgatggt tccaaccaag
    32341 ctatcaccct catagacttc caaaccgata atctcgtgat agtaaaattc accatcgtct
    32401 aggtcattca aatcttcctc agcgaccttg agactgtatc ccttgtactt ttcgatagta
    32461 ttgatatggt acatatcttt gaatttaata atgtcaaagt tcttctgttt acggtggcta
    32521 gcgatggtca ctgtttggac aaactgatct ttttcatcaa acaaagccag ctcagctcct
    32581 tttttaaacc gttcttctgc aaaatccgtc acagacaaga ctcgcatctc accctgtaat
    32641 ccctgcgtat taacgatttt cccaacatta aagtagttca tcttgtctcc tgtaatctcc
    32701 ttttttccat cttattctga caattctcga ataatagccg caattttttc cgattctgac
    32761 cattgtaaat aatggcgatt ccctcctaaa atgagtttag tattggaagt ccaatattct
    32821 gattctctgt actctttttc tctataagtc tgacaaaaaa taaaaacagg gacatgagat
    32881 tctatcgaca aattctcaaa atcttcctca gtaatctctc cagatatctg aaattctgga
    32941 tcttgatttt ccaactctaa gcctttttct tgcattaatt cccagatttt tttattcgtt
    33001 tcaggactaa atgttgcttg agttaagttc ttaaaataaa gttcaggacc acactcgtca
    33061 atcagcctca tctgctcttc catttctgga taaggatttt ctgaaaaatc agcaaacatg
    33121 acttttttag ttgtcggttc aattgctact aaagtctgac acttaattgg tttctcgagt
    33181 aatttgcaag ctaaaattcc actccaacta tgtacacaaa gtatatattc agaaattcct
    33241 aattcttcaa gtgcttcata aaccacatct gcaagattat ctagattttt tccagcttgg
    33301 tcatgaatcg gactcctacc tgtgttcgga aaatcaattg tcaaataacc aattgtagga
    33361 ggaggttttt caagtataag tgaaaaattt tcataacttg gtagcaaacc tgctccgttt
    33421 agacaaacta gcactttctt ttgcttttga taagtaacag agaggctacc aatttctgta
    33481 gatacttcaa acctcttcat aaagaaatcc actgattcta tataatgaat tattaaaaat
    33541 ccttatcctt tattttatca cgttccaagg attttctcaa gttggaggaa ggggacaata
    33601 tctctacttt cccttcaaga atccttccaa attatgttta tgttggtaat taacggctgc
    33661 ggttttgtct ttctcaaaga cagtcttggt aaggtcaata tgattaatag ctacgattgc
    33721 gacggtgtag taaatgatat cagccagttc tctggcaagt tcctcgttcg aatcctatcc
    33781 cttcttttcg accagagcgc ctattcaaaa cctcggctac ttctccgact tcctccacta
    33841 acttcataaa gagaccttca tcagtccgag actgctgtta atgttcgatt aagtagtctt
    33901 ggaattgcct aaacgttcaa tcttttatag tatattgaaa ctagaatagt acacctttac
    33961 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct attcttgttt
    34021 cattttacta tatcttctat tccacacaaa aaagcgagac atccgtcccg cccttcttat
    34081 ttttcgtcaa taacgattct tacttttttg tattcagttg ggacagagta gacaatcgtt
    34141 cttatcgcag aaatagtgcg acccttacga ccgattacac gacccacatc gctttgatca
    34201 agattcaaat gatattccaa aaattctggt gtatcctcaa tcttgatagt taaggcatct
    34261 ggttgtgaaa ttaagggttt cacaatcgca ataatgagat tttcaatcgt atccatctgt
    34321 caacctactt taaacttatt ttgagaattt agaatcgtgg aattttttca atacgccttc
    34381 ttttgaaagg atgttacgta ctgtgtctga aggttgagct ccattagcca accatgcaag
    34441 aacgcggtct tctttcaaag ttacttggtt ttcagcaaca agtgggttgt aagttccaac
    34501 tgtttcgatg aaacgtccgt cacgtggtga acgtgaatct gctacgttga tacggtagaa
    34561 aggttttttc ttagaaccca tacgagtcaa acggatttta actgccattt ttaaagtctc
    34621 atttctttaa ttttttattt cggtgaaata gctgagctat ttagcacatg ttctattata
    34681 gcagatttct ggcatgtgtc aagaaaaaaa cttgacagac tataaatttc ttaaagtatt
    34741 tagaaatttg ttagaatagg aaa
//
LOCUS       DAVMAF010000015        34474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMAF010000015 DAVMAF010000000
VERSION     DAVMAF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.015"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            572..1024
                     /locus_tag="VYO97_000712"
     CDS             572..1024
                     /locus_tag="VYO97_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1753544.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1043..2380
                     /locus_tag="VYO97_000713"
     CDS             1043..2380
                     /locus_tag="VYO97_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1753545.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLGSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2459..3343
                     /locus_tag="VYO97_000714"
     CDS             2459..3343
                     /locus_tag="VYO97_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1753546.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3343..4176
                     /locus_tag="VYO97_000715"
     CDS             3343..4176
                     /locus_tag="VYO97_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1753547.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4194..6287
                     /gene="nanB"
                     /locus_tag="VYO97_000716"
     CDS             4194..6287
                     /gene="nanB"
                     /locus_tag="VYO97_000716"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1753548.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVREKGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6299..7402
                     /locus_tag="VYO97_000717"
     CDS             6299..7402
                     /locus_tag="VYO97_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1753549.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7576..8274
                     /locus_tag="VYO97_000718"
     CDS             7576..8274
                     /locus_tag="VYO97_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1753550.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8293..9810
                     /locus_tag="VYO97_000719"
     CDS             8293..9810
                     /locus_tag="VYO97_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1753551.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9935..11263
                     /locus_tag="VYO97_000720"
     CDS             9935..11263
                     /locus_tag="VYO97_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753552.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11515..12381
                     /locus_tag="VYO97_000721"
     CDS             11515..12381
                     /locus_tag="VYO97_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1753553.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12397..13236
                     /locus_tag="VYO97_000722"
     CDS             12397..13236
                     /locus_tag="VYO97_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1753554.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13508..13960
                     /locus_tag="VYO97_000723"
     CDS             13508..13960
                     /locus_tag="VYO97_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1753555.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYASMEEKVRKYLFKILID"
     gene            14102..14410
                     /locus_tag="VYO97_000724"
     CDS             14102..14410
                     /locus_tag="VYO97_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753556.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAERVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKIPNNGRINF
                     "
     gene            14697..15341
                     /locus_tag="VYO97_000725"
     CDS             14697..15341
                     /locus_tag="VYO97_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1753557.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15510..16427
                     /locus_tag="VYO97_000726"
     CDS             15510..16427
                     /locus_tag="VYO97_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1753558.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16445..17329
                     /locus_tag="VYO97_000727"
     CDS             16445..17329
                     /locus_tag="VYO97_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1753559.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17345..18196)
                     /locus_tag="VYO97_000728"
     CDS             complement(17345..18196)
                     /locus_tag="VYO97_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1753560.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18396..20453
                     /gene="pbp2b"
                     /locus_tag="VYO97_000729"
     CDS             18396..20453
                     /gene="pbp2b"
                     /locus_tag="VYO97_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1753561.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20464..21060
                     /gene="recR"
                     /locus_tag="VYO97_000730"
     CDS             20464..21060
                     /gene="recR"
                     /locus_tag="VYO97_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1753562.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21236..22279
                     /locus_tag="VYO97_000731"
     CDS             21236..22279
                     /locus_tag="VYO97_000731"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1753563.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22363..23736
                     /locus_tag="VYO97_000732"
     CDS             22363..23736
                     /locus_tag="VYO97_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1753564.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLAAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELGITDLVERKDSLTFKVNFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23723..24334
                     /locus_tag="VYO97_000733"
     CDS             23723..24334
                     /locus_tag="VYO97_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1753565.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24425..25129
                     /locus_tag="VYO97_000734"
     CDS             24425..25129
                     /locus_tag="VYO97_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1753566.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25348..26721
                     /gene="ftsA"
                     /locus_tag="VYO97_000735"
     CDS             25348..26721
                     /gene="ftsA"
                     /locus_tag="VYO97_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1753567.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26738..27997
                     /gene="ftsZ"
                     /locus_tag="VYO97_000736"
     CDS             26738..27997
                     /gene="ftsZ"
                     /locus_tag="VYO97_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1753568.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28002..28673
                     /locus_tag="VYO97_000737"
     CDS             28002..28673
                     /locus_tag="VYO97_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1753569.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28683..29222
                     /locus_tag="VYO97_000738"
     CDS             28683..29222
                     /locus_tag="VYO97_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1753570.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29222..29485
                     /locus_tag="VYO97_000739"
     CDS             29222..29485
                     /locus_tag="VYO97_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1753571.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29482..30267
                     /locus_tag="VYO97_000740"
     CDS             29482..30267
                     /locus_tag="VYO97_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1753572.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVFSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30276..31064
                     /gene="divIVA"
                     /locus_tag="VYO97_000741"
     CDS             30276..31064
                     /gene="divIVA"
                     /locus_tag="VYO97_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1753573.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31412..34204
                     /locus_tag="VYO97_000742"
     CDS             31412..34204
                     /locus_tag="VYO97_000742"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1753574.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAAVSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10602 a         6064 c         7497 g        10311 t
ORIGIN      
        1 attgaaaacg gtaacaaaac taatttagag aatgaaatat agagtatttc tctcttaaaa
       61 gtttttggtg aaacgagatg tagaaaggag atttagccaa agagtctatt agtgctagaa
      121 taatagatta gaattatttt agaaaaacga agtgagcagc ttataaattc aagtccccaa
      181 atagattcat actagtatct tttgcaaaaa ataaagggcg acttccttca tgaatatcaa
      241 tttcatctat aaggaaggta gctaattgaa ctaacttatt tattctgttt gtcgctagaa
      301 aaatcagacc tccttgtgaa gattgaggag atactgaatg aaaatcaaag aagaaactag
      361 caagctagta gcagattgcc caaaacaccg ctttgaggtt gtagataaga ctgacgtata
      421 taatccaagg tgaagcgact gtggtttgaa gagattttca aagagtatag gctagagagt
      481 agtgttttta tgtccttcta gtagaaaatg ctagacagaa gaatggggaa cttggatagg
      541 aaaaatagat tgagaaagga ggttagaaga gatgattatt acaaaaatta gccgtttagg
      601 aacttatgtg ggagtaaatc cacattttgc aacattaata gattttctag aaaaaacagg
      661 actagaaaat ttaacagaag gttcgattgc tatcgatggt aatcgattgt ttgggaattg
      721 ctttacttat ctagcagatg gtcaagcagg ggctttcttt gaaacacacc aaaaatattt
      781 ggatattcat ttagttttgg aaaacgaaga agccatggct gttacatcgc cggaaaatgt
      841 aagcgttacc caagaatatg atgaagagaa agatattgaa ttatacacag ggaaagtgga
      901 acagttggtt catttgagag ctggcgaatg cctcatcact tttccagaag atttacatca
      961 acccaaggtt cgtataaatg atgaacctgt gaaaaaagtt gtctttaaag ttgcgatttc
     1021 ttaatgtaga aagagaagaa cgatgaaaaa aatgagaaag tttttatgtc tagctggaat
     1081 tgcgctagcg gctgttgcct tggtagcttg ttcaggaaaa aaagaagcta caactagtac
     1141 tgaaccacca acagaattat ctggtgagat tacaatgtgg cactccttta ctcaaggacc
     1201 ccgtttagaa agtattcaaa aatcagcaga tgctttcatg caaaagcatc caaaaacgaa
     1261 aatcaagatt gaaacatttt cttggaatga cttctatact aaatggacta caggtttagc
     1321 aaatggaaat gtgccagata tcagtacagc tcttcctaac caagtaatgg aaatggtcaa
     1381 ctcagatgct ttggttccgc taaatgattc tatcaagcgt attggacaag ataaatttaa
     1441 cgaaactgcc ttaaatgaag caaaaatcgg agatgattac tactctgttc ctctttattc
     1501 acatgcacaa gtcatgtggg ttagaacaga tttgttaaaa gaacataata ttgaggttcc
     1561 taaaacttgg gatcaactct atgaagcttc taaaaaattg aaagaagctg gagtttatgg
     1621 cttgtctgtt ccgtttggaa caaatgactt aatggcaaca cgtttcttga acttctacgt
     1681 acgtagtggt ggaggaagcc tcttaacaaa agatcttaaa gcagacttga caagccaact
     1741 tgctcaagat ggtattaaat actgggttaa attgtataaa gaaatctcac ctcaagattc
     1801 tttgaacttt aatgtccttc aacaagctac cttgttctat caaggaaaaa cagcatttga
     1861 ctttaactct ggcttccata tcggaggaat taatgccaac agtcctcaat tgattgattc
     1921 gattgatgct tatcctattc caaaaatcaa agagtctgat aaagaccaag gaattgaaac
     1981 ctcaaacatt ccaatggttg tttggaaaaa ttcaaaacat ccagaagttg ctaaagcatt
     2041 cttagaagca ctttataatg aagaagacta cgttaaattc cttggttcaa ctccagtagg
     2101 tatgttgcca actattaagg ggattagcga ttctgcagcc tataaagaaa atgaaactcg
     2161 taagaaattt aaacatgctg aagaagtaat tactgaagct gttaaaaaag gtactgctat
     2221 tggttatgaa aatggaccaa gtgtacaagc tggtatgtta actaaccaac acattattga
     2281 acaaatgttc caagatatca ttacaaatgg aacagatcct atgaaagcag caaaagaagc
     2341 agaaaaacaa ttaaacgatt tatttgaggc tgttcagtag atgtaaaaga ctagaaaata
     2401 ggtgggctag tgatctgaaa agctctagcc caatcttgta aaagaaggga gaaggagaat
     2461 ggttaaagaa cgtaatttaa ctcgctggat atttgttttg ccagctatga ttatcgtagg
     2521 attactcttt gtttatccgt ttttctcgag tattttttat agctttacca ataagcattt
     2581 gattatgcct aattataaat ttgttggttt ggctaactat aaagctgtgc tatcagatcc
     2641 caacttcttt aatgcgttct ttaattcaat taagtggacc gttttctcat tagttggtca
     2701 agttttagta gggtttgtat tggctttagc tcttcacaga gtacgccact tcaagaaatt
     2761 atataggacc ttattgattg ttccttgggc atttcctacc atcgttattg ccttttcttg
     2821 gcagtggatt ctaaacgggg tttatggcta cttacctaat ctaatcgtaa aattaggttt
     2881 aatggaacat acacctgcat ttttgacaga tagtacatgg gcattcctat gtttggtgtt
     2941 tatcaacatt tggtttggag caccaatgat tatggttaat gtgctttcag ctttgcaaac
     3001 agtaccagaa gaacaatttg aggctgctaa gatagatggt gcttcaagtt ggcaggtgtt
     3061 caagtttatc gtctttccac atattaaagt ggttgtagga cttctagttg ttttgagaac
     3121 tgtatggatc tttaataact ttgacattat ctacctcatt actggtggtg gaccagccaa
     3181 tgctacaacg acgcttccaa tttttgctta caacctgggc tggggaacta aattgttggg
     3241 tcgtgcttca gcagttacag tattgctctt tatcttcttg gtggcgattt gctttatcta
     3301 ctttgctatc atcagtaagt gggaaaagga gggtagaaaa taatgaagaa gaaatccagt
     3361 atttatttag atattctctc acatgtactt ttagttggtg cgaccatcgt tgcagttttc
     3421 ccattggtat ggattatcat atcttctgtc aaagggaaag gggaattaac tcagtatcca
     3481 acacgatttt ggcctgaaca gtttacatta gattatttca ctcatgttat caacgatttg
     3541 cacttcattg ataacattcg aaacagttta atcattgcct tggctacaac ccttattgcg
     3601 attattattt ctgctatggc agcctatggt attgttcgat tctttcctaa attgggagca
     3661 atcatgtcga gactactcgt cattacctac attttcccac caattttgtt agcaattccc
     3721 tattcaattg ccattgctaa agttgggtta acaaatagtt tatttggctt gatgatggtt
     3781 tatctatctt ttagtgttcc atatgcagtt tggctcttag ttggattttt ccaaacagtt
     3841 ccaattggaa ttgaagaagc ggctagaatt gatggtgcaa ataaatttgt tacgttttat
     3901 aaagttgtgc taccgattgt agcaccaggt attgtagcaa cagctattta tacatttatc
     3961 aatgcttgga atgaattcct gtatgccttg attttgatta acaatacagg aaagatgaca
     4021 gtagcagtag cccttcgttc acttaatggt tcagaaatac tagactgggg agatatgatg
     4081 gcagcgtctg ttattgtagt tcttccatca attattttct tctctatcat ccaaaataag
     4141 attgcaagtg gattatcaga aggatctgtg aagtagacga aagaaggaaa aaaatgaata
     4201 aaagaggtct ttattcaaaa ctaggaattt ctgttgtagg cattagtctt ttaatgggag
     4261 tccccacttt gattcatgcg aatgaattaa actatggtca actgtccata tctcctattt
     4321 ttcaaggagg ttcatatcaa ctgaacaata agagtataga tatcagccct ttgttattag
     4381 ataaattgtc tggagagagt cagacagtag taatgaaatt taaagcagat aaaccaaact
     4441 ctcttcaagc tttgtttggc ctatctaata gtaaagcagg ctttaaaaat aattactttt
     4501 caattttcat gagagattct ggtgagatag gtgtagaaat aagagacgcc caaaagggaa
     4561 taaattattt attttctaga ccagcttcat tatggggaaa gcataaagga caggcagttg
     4621 aaaatacact agtatttgta tctgattcta aagataaaac atacacaatg tatgttaatg
     4681 gaatagaagt gttctctgaa acagttgata catttttgcc aatttcaaat ataaatggta
     4741 tagataaggc aacactagga gctgttaatc gtgaaggtaa ggaacattac ctcgcaaaag
     4801 gaagtattgg tgaaatcagt ctatttaaca aagcaattag tgatcaggaa gtttcaaata
     4861 ttcccttgtc aaatccattt cagttaattt tccaatcagg agattctact caagctaact
     4921 attttagaat accgacacta tatacattaa gtagtggaag agttctatca agtattgatg
     4981 cacgttatgg tgggactcat gattctaaaa gtaagattaa tattgccact tcttatagtg
     5041 atgataatgg gaaaacgtgg agtgagccaa tttttgctat gaagtttaat gactatgagg
     5101 agcagttagt ttactggcca cgagataata aattaaagaa tagtcaaatt agtggaagtg
     5161 cttcattcat agattcatcc attgttgaag ataaaaaatc tgggaaaacg atattactag
     5221 ctgatgttat gcctgcgggt attggaaata ataatgcaaa taaagccgac tcaggtttta
     5281 aagaaataaa tggtcattat tatttaaaac taaagaagaa tggagataac gatttccgtt
     5341 atacagttag agaaaagggt gtcgtttatg atgaaacaac taataaacct acaaattata
     5401 ctataaatga taagtatgaa gttttggagg gaggaaagtc tttaacagtc gaacaatatt
     5461 cggttgattt tgatagtggc tctttaagag aaaggcataa tggaaaacag gttcctatga
     5521 atgttttcta caaagattcg ttatttaaag tgactcctac taattatata gcaatgacaa
     5581 ctagtcagaa tagaggagag agttgggaac aatttaagtt gttgcctccg ttcttaggag
     5641 aaaaacataa tggaacttac ttatgtcccg gacaaggttt agcattaaaa tcaagtaaca
     5701 gattgatttt tgcaacatat actagtggag aactaaccta tctcatttct gatgatagtg
     5761 gtcaaacatg gaagaaatcc tcagcttcaa ttccgtttga aaatgcaaca gcagaagcac
     5821 aaatggttga actgagagat ggtgtgatta gaacattctt tagaaccact acaggtaaga
     5881 tagcttatat gactagtaga gattctggag aaacatggtc gaaagtttcg tatattgatg
     5941 gaattcaaca aacttcatat ggcacacaag tatctgcaat taaatactct caattaattg
     6001 atggaaaaga agcagtcatt ttgagtacac caaattctag aagtggccgt aagggaggcc
     6061 aattagttgt cggtttggtc aataaagaag atgatagtat tgattggaga taccactatg
     6121 atattgattt gccttcgtat ggttatgcct attctgcgat tacagaattg ccaaatcatc
     6181 acataggtgt actgtttgaa aaatatgatt cgtggtcgag aaatgaattg catttaagca
     6241 atgtagttca gtatatagat ttggaaatta atgatttaac aaaataaagg agaaaaacat
     6301 ggttaaatac ggtgttgttg gaacagggta ttttggagct gaattggctc gctacatgca
     6361 aaagaatgat ggagcagaga ttactcttct ctatgatcca gataatgcag aggcgattgc
     6421 agaagaattg ggagcaaaag tagcaagttc cttagatgag ttggtttcta gcgatgaagt
     6481 agattgtgtt atcgtcgcaa ctccaaataa tcttcataag gaaccggtta ttaaggctgc
     6541 acagcatggt aaaaatgttt tctgtgaaaa accaattgcg ctttcttatc aagattgtcg
     6601 cgagatggta gatgcgtgta aagaaaacaa tgtaaccttt atggcaggac atattatgaa
     6661 tttctttaat ggtgttcatc atgcaaaaga actcattaat caaggagtta tcggagacgt
     6721 tctatattgt catacagctc gtaatggttg ggaagaacaa caaccgtcag tatcatggaa
     6781 aaaaattcgt gaaaaatcag gtggtcactt gtatcaccac atccatgaat tggattgcgt
     6841 tcaattcctt atggggggca tgcctgaaac tgtaaccatg acaggtggaa atgtggccca
     6901 tgaaggtgaa catttcggtg atgaagatga tatgattttt gtcaatatgg aattttctaa
     6961 taagcgtttt gccttgttag aatggggttc agcttatcgt tggggtgaac attatgtctt
     7021 aatccaagga agcaaaggtg ccatccgctt agacttattc aactgtaaag gaactcttaa
     7081 gctagatggg caagaaagct atttcttgat tcacgaatcg caagaagaag atgatgatcg
     7141 gactcgtatc tatcatagta cagagatgga tggagcaatt gcttatggta aaccaggtaa
     7201 acgtactcca ttatggctat catctgtcat tgataaagaa atgcgctatc tgcatgagat
     7261 tatggaagga gctccagtat cagaagaatt tgcaaaactt ttgacaggtg aagctgccct
     7321 agaagcaatt gctactgcag atgcttgtac ccagtctatg tttgaagatc gcaaagtaaa
     7381 attgtcagaa attgtaaaat aaattttggt attctcctat ttataggtcg acttgctcct
     7441 ctgaaagtac ttttagagga gctgtttgac tttgctagtt tttgaaactg aaatctatta
     7501 tactacaaac tattgaaagc gttttaattt taaggtataa taatctcata gaaataaaga
     7561 aaaggaggaa agaggatgcc acagattagc aaagaagcct tgattgagca aatcaaagat
     7621 ggaatcatcg tttcttgtca ggctcttcct catgaaccgc tttatacaga agcgggaggg
     7681 gtgattccct tgctggtcaa agcggctgag caaggtggag cagtcggtat ccgagcaaac
     7741 agtgttcgcg atatcaagga aattaaggaa gtcactaaac ttccaatcat tgggattatc
     7801 aaacgtgatt atccacctca ggaacccttc atcacggcta ctatgaaaga agttgatgaa
     7861 ttggcagaac tggacatcga ggtgattgct ctggattgta ccaagcgtga acgctacgat
     7921 ggtttggaaa ttcaagagtt cattcgtcag gttaaggaga aatatcctaa tcagcttttg
     7981 atggctgata ctagtatctt cgaagaaggg ctagcagctg tagaagcagg aattgacttt
     8041 gtcggaacaa ccttatcagg ctacacatcc tacagtccaa aagtagacgg tccagatttt
     8101 gaattgatta agaaactctg tgatgctggt gtagatgtca ttgcagaagg aaaaattcat
     8161 acaccagaac aagccaaaca aatccttgaa tatggagtgc gaggcatcgt tgttggtggc
     8221 gccattacta gaccaaaaga gattacagaa cgcttcgttg ctagtcttaa ataagatgtg
     8281 agggggagtt ttatgtttaa agttttacaa aaagttggaa aagcttttat gttacctata
     8341 gctatacttc ctgcagcagg tctacttttg gggattggtg gtgcactttc aaacccaacc
     8401 acgatagcaa cttatccaat actagacaat agtatttttc aatcaatatt ccaagtaatg
     8461 agctctgcag gagaggttgt attcagtaat ttgtcactac ttctctgtgt gggattatgt
     8521 attggcttag cgaaacgaga taaaggaacc gctgcgttag caggagtaac tggttactta
     8581 gttatgactg caacgatcaa agctttggta aaacttttta tggcagaagg atctgcaatt
     8641 gatactggag ttattggagc attagttgtc ggaatagttg ccgtatattt gcacaaccga
     8701 tataacaata ttcaattacc ttccgcttta ggattctttg gaggttcacg cttcgttcct
     8761 attgttacat cgttctcttc tatcttgatt ggctttgtct tctttgttat ttggccacct
     8821 ttccaacaac ttcttgtttc tacaggtgga tatatttctc aggcgggtcc aattggaact
     8881 tttctatatg gatttttaat gagactttct ggagcagtag gcttacatca tataatttac
     8941 cctatgtttt ggtatactga acttggtggt gttgaaactg ttgcaggaca aacagtggtt
     9001 ggagctcaaa aaatattttt tgctcaatta gccgatttgg cccattctgg attatttaca
     9061 gaaggaacaa ggttttttgc aggtcgtttc tcaacaatga tgttcggttt accggctgcc
     9121 tgtttagcga tgtaccatag tgttcctaaa aatcgtcgta aaaaatacgc gggtttgttt
     9181 tttggagttg ctttaacatc ttttattacc ggtattacag aaccaattga atttatgttt
     9241 ctattcgtca gtccggttct atatgttgtt cacgcattcc ttgatggtgt tagcttcttt
     9301 attgcagacg tcttaaatat ttcaatagga aacacatttt caggaggtgt aatcgatttc
     9361 actttatttg gaattttgca ggggaacgct aagacgaatt gggttcttca gattccattt
     9421 ggacttattt ggagtgtttt gtattatatt atttttagat ggttcattac tcaattcaac
     9481 gttctaacgc cagggcgagg agaagaagta gattctaaag aaatttctga atccgcagat
     9541 tcaacttcaa atactgcaga ttatttaaaa caggatagcc tacaaattat cagagccttg
     9601 ggtggatcaa ataatataga agatgtagat gcttgtgtga cacgtttacg tgtagctgta
     9661 aaagaagtta atcaagttga taaagcactt ttaaaacaaa ttggtgcagt tgatgtctta
     9721 gaagtgaagg gtggcattca agcaatctat ggagcaaaag caatcttata taaaaatagt
     9781 attaatgaaa ttttaggtgt agatgattaa gtacttactg acttaataaa aaacagagga
     9841 gagtgatgga tgagtaggat gaaatgaaat cgcatacaag aaataaagaa ctcattatcc
     9901 aagttggata cgcttattac ataggagaat acaaatgaaa tttagaaaat tagcttgtac
     9961 agtacttgcg ggtgctgcgg ttcttggtct tgctgcttgt ggcaattctg gcggaagtaa
    10021 agatgctgcc aaatcaggtg gtgacggtgc caaaacagaa atcacttggt gggcattccc
    10081 agtatttacc caagaaaaaa ctggtgacgg tgttggaact tatgaaaaat caatcatcga
    10141 agcgtttgaa aaagcaaacc cagatataaa agtgaaattg gaaaccatcg acttcaagtc
    10201 aggtcctgaa aaaatcacaa cagccatcga agcaggaaca gctccagacg tactctttga
    10261 tgcaccagga cgtatcatcc aatacggtaa aaacggtaaa ttggctgagt tgaatgacct
    10321 cttcacagat gaatttgtta aagatgtcaa caatgaaaac atcgtacaag caagtaaagc
    10381 tggagacaag gcttatatgt atccgattag ttctgcccca ttctacatgg caatgaacaa
    10441 gaaaatgtta gaagatgctg gagtagcaaa ccttgtaaaa gaaggttgga caactgatga
    10501 ttttgaaaaa gtattgaaag cacttaaaga caagggttac acaccaggtt cattgttcag
    10561 ttctggtcaa gggggagacc aaggaacacg tgcctttatc tctaaccttt atagcggttc
    10621 tgtaacagat gaaaaagtta gcaaatatac aactgatgat cctaaattcg tcaaaggtct
    10681 tgaaaaagca actagctgga ttaaagacaa tttgatcaat aatggttcac aatttgacgg
    10741 tggggcagat atccaaaact ttgccaacgg tcaaacatct tacacaatcc tttgggcacc
    10801 agctcaaaat ggtatccaag ctaaactttt agaagcaagt aaggtagaag tggtagaagt
    10861 accattccca tcagacgaag gtaagccagc tcttgagtac cttgtaaacg ggtttgcagt
    10921 attcaacaat aaagacgaca agaaagtcgc tgcatctaag aaattcatcc agtttatcgc
    10981 agatgacaag gagtggggac ctaaagacgt agttcgtaca ggtgctttcc cagtccgtac
    11041 ttcatttgga aaactttatg aagacaaacg catggaaaca atcagcggct ggactcaata
    11101 ctactcacca tactacaaca ctattgatgg atttgctgaa atgagaacac tttggttccc
    11161 aatgttgcaa tctgtatcaa atggtgacga aaaaccagca gatgctttga aagccttcac
    11221 tgaaaaagcg aacgaaacaa tcaaaaaagc tatgaaacaa tagtccttag ttattctata
    11281 aaaagtagtt ttttaaagaa cctaagagtg tataccccct tttccctcta cacagatagt
    11341 gtaagaaaag ggggcttttg tttaaaatgt aagaaactgt cacgaaatta aaatgaagtt
    11401 cttacataag cgaatcataa aaaatttcat tttgatttta aaacagttca agaaagtcaa
    11461 aaaattattc tatttgaaag agaggtgccg actgtgaaag tcaataaaat ccgtatgcgg
    11521 gaaacagtga tttcctacgc tttcctagca ccagtattat tcttctttgt catctttgtg
    11581 ttggctccga tggtgatggg cttcattaca agtttcttta actactcaat gactaaattt
    11641 gagtttgtag gcttggataa ctatatccgt atgtttaaag atcctgtctt tacaaaatct
    11701 ctgattaaca cagttatttt ggttattgga tctgtaccag ttgttgttct attctcactc
    11761 tttgtagcat ctcagaccta tcatcaaaat gtcattgcca gatccttcta ccgtttcgtc
    11821 ttcttccttc ctgttgtaac gggtagtgtt gccgtgacag ttgtttggaa atggatttat
    11881 gacccactat cagggattct aaactttgtc cttaagtcca gccacatcat cagccaaaac
    11941 atttcttggt tgggagataa aaactgggca ttgatggcga ttatgattat tctcttgacc
    12001 acttcagttg gtcagcccat catcctttat atcgctgcca tggggaatat tgacaattca
    12061 ctggttgaag cggcgcgtgt tgatggtgca actgagtttc aagttttttg gaagattaaa
    12121 tggccaagcc ttcttccaac aactctttat attgcaatca tcacaacaat taactcattc
    12181 cagtgtttcg ccttgattca gcttttgaca tctggtggtc caaactactc aacaagtacc
    12241 ttgatgtact acctttacga aaaagccttc caattgacag aatacggcta tgccaacaca
    12301 attggtgtct tcttggcagt catgattgct atcgtaagct ttgttcaatt taaagtactt
    12361 ggaaacgacg tagaatacta aagaaaggag acagctatgc aatctacaga aaaaaaacca
    12421 ttaacagcct ttactgttat ttcaacaatc attttgctct tgttgactgt gctgttcatc
    12481 tttccattct actggatttt gacaggggca ttcaaatcac aacctgatac aattgttatt
    12541 cctcctcagt ggttccctaa aatgccaacc atggaaaact tccaacaact catggtgcag
    12601 aaccctgcct tgcaatggat gtggaactca gtatttatct cattggtaac catgttctta
    12661 gtttgtgcaa cctcatctct agcaggttat gtattggcta aaaaacgttt ctatggtcaa
    12721 cgcattctat ttgctatctt tatcgctgct atggcgcttc caaaacaagt tgtccttgta
    12781 ccattggtac gtatcgtcaa cttcatggga atccacgata ctctctgggc agttatcttg
    12841 cctttgattg gatggccatt cggtgtcttc ctcatgaaac aatttagtga aaatattcct
    12901 acagagttgc ttgaatcagc taaaatcgac ggttgtggtg agattcgtac cttctggagt
    12961 gtagccttcc cgattgtgaa accagggttt gcagcccttg caatctttac cttcatcaat
    13021 acttggaatg actacttcat gcaattggta atgttgactt cacgtaacaa tttgaccatc
    13081 tcacttgggg ttgcgaccat gcaggctgaa atggcaacca actatggttt gattatggca
    13141 ggagctgccc ttgctgctgt tccaatcgtc acagtcttcc ttgtcttcca aaaatccttc
    13201 acacagggta ttactatggg agcggtcaaa ggataatact ctgcgaaaat ctcttcaaac
    13261 tacgtcagct tcaccttgcc atacttaagt attgcctgcg gttagcttcc tagtttgttc
    13321 ttcaattttc attgagtata ggaaaatcaa tctatcaaga tacagaagta tattttatag
    13381 atttagagaa tatagaggtt ataagtgtct acaaaatgga gggtatgcag ttactttatg
    13441 aagttttgtc agacacttat aaacttaaga atggttttag ttaactatca gaaacgaagg
    13501 aaagagtatg atttttgacg atttgaaaaa catcaccttt tacaaaggga ttcatcccaa
    13561 tttagacaag gctatcgact acctctacca acatcgtaaa gattcattcg aattaggaaa
    13621 gtatgagatt gatggggata aagtctttct agttgttcag gaaaatgtcc tcaatcaagt
    13681 tgagaataat caatttgaac accataagaa ctatgcagat ttgcatttgc tgatagaagg
    13741 gcatgaatat tcgagctacg gttcacgtat caaagacgag gcagtagcat tcgacgaagc
    13801 gactgacatt ggctttgttc attgtcatga acactacccc ctcttgttgg gttatcacaa
    13861 ttttgcgatt ttcttcccag gtgagccaca tcagccaaat ggttatgcaa gcatggaaga
    13921 aaaggttcgg aaatatctct ttaaaatttt gattgattaa aaataggatg aattgttttt
    13981 ttgtaaagct ttgacaatac tctaccatga aattgatctt tgtgaggtag ggaaatgaga
    14041 ataaaatatt taaaaattgg tatcttcttg tcagtctgtg tatgctacaa gagctagttt
    14101 cttggatgga cagggaatta cagttgatga gatggcttgg ataattaggg gcattgtgaa
    14161 tgcattgatt ggtagataca taaaattagg tacttatgcg gctaagtatg gtattagtat
    14221 ggcgcgttct atcttaaata gagcagctgc aactgcggca gaaagagtag gattattgac
    14281 taagattttt ggatggattt tacgaatagc tgtgctcgca gctgatgtat atggtaattt
    14341 tgccaacaat attgctgtag cttgggatgc acatgataaa atccctaaca atggtcgtat
    14401 aaacttttaa aatgcgagaa tgaaagcact ttgtattttt ttattgaata tgttagcttg
    14461 gacagtgctt gcaatgataa ttcgtggagg gctagatgga tttgataggc atacttggag
    14521 taatatttta gctagtgcgt tgtcctcgct atacatacat tatatatcca tagatagaaa
    14581 aagaaaaaag tccaaaagaa aaaagaaaaa atagatttgt tcatggtagg gacttatgaa
    14641 agctttacta acaaaaaaga aaacagttta caaagaaaaa tgatggagga gtaaacatgg
    14701 cacaaaaagg agtaagcctt atcaaggcag catttgatac agataacttt cttatgcgtt
    14761 ttagtgagaa agtcttggac atcgtgacag ccaatcttct ttttgtcgtc tcttgtttgc
    14821 ccatcgtgac gattggagtg gctaaaatca gcctctacga gaccatgttt gagattaaga
    14881 agagcagacg ggtgcctgtt tttaaaatct atctaagatc tttcaagcaa aatctgaaac
    14941 taggtcttca gttaggtctg cttgagttgg gcattgtttc attaagcctc ctagatcttt
    15001 atctcttttg gggtcaaaca gctctgccct ttcaattgct gaaagctatt tgtctaggca
    15061 ttctgatttt ccttacgatt gtgatgttgg ctagctatcc aatcgcggca cgttatgatt
    15121 taacttggaa agaagtgtta caaaaagggc ttatcttggc aagttttaac tttccttggt
    15181 tcttcctcat gttagccatt cttgtcctca ttgtgatggt tctttatctg tctgccttca
    15241 gtctactttt aggtggctca gtcttcctgc tttttgggtt tggactattg gtttttatcc
    15301 agactggatt gatggagaaa attttcgcaa aataccaata ggagccttat ttctgaaact
    15361 actttcaaag gctccaaacg ctattctata agcgagaaac taaaatctaa gtgtctataa
    15421 gatattgaaa gccttttcgg taaagtgtat actaaagaag taaagaaaaa tctgctttag
    15481 cagaaaataa aaataatagg agaaaatcta tgtcagattt gaaaaaatac gaaggtgtca
    15541 ttccagcctt ctacgcatgt tatgatgatc aaggagaagt aagcccagaa cgtacgcgtg
    15601 ccttggttca atacttcatt gataaaggtg ttcaaggtct ttatgtcaat ggttcttctg
    15661 gtgaatgtat ctaccaaagc gttgaagatc gcaagttgat tttggaagaa gtcatggcgg
    15721 tagccaaagg taaattgacc attattgccc atgttgcttg caataatact aaagatagta
    15781 tggaacttgc tcgccatgct gaaagcttgg gagtagatgc tattgcaacg attccaccaa
    15841 tttatttccg cttgccagaa tactcagttg ccaaatactg gaacgatatc agttctgcag
    15901 ctccaaacac agactacgtg atttacaaca ttcctcaatt ggcaggggtt gctttgactc
    15961 caagccttta cacagaaatg ttgaaaaatc ctcgtgttat cggtgtgaag aactcttcta
    16021 tgccagttca agatatccaa acctttgtga gccttggtgg ggaagaccat atcgtcttta
    16081 acggtccaga tgaacagttc ctaggtggtc gcctcatggg tgccaaagct ggtatcggtg
    16141 gtacttatgg tgctatgcca gaactcttct tgaaactcaa ccaattgatt gcggataagg
    16201 acctagaaac agcgcgtgaa ttgcagtatg ctatcaacgc aatcattggt aaactcactt
    16261 ctgctcatgg aaatatgtac ggtgtcatta aagaagtctt gaaaatcaat gaaggcttga
    16321 atattggatc tgttcgttca ccattgacac cagtgactga agaagatcgt ccagttgtag
    16381 aagcggctgc tgccttgatt cgtgaaacca aggagcgctt cctctaatct aaaaggaggt
    16441 atttatgaca tactacgttg caattgatat cggtggaacc aacatcaagt atggtttggt
    16501 tgatcaagag gggcaacttc ttgaatcgca tgaaatgcca actgaggcgc ataagggtgg
    16561 acctcatatc ttacaaaaga ccaaagatat cgtagctagt tatttagaaa aaggcccagt
    16621 agcaggtgtt gccatatctt ctgctgggat ggtggatccg gataagggtg agattttcta
    16681 tgctgggcct caaatcccta actacgcagg cacccagttc aaaaaggaaa tcgaagaaag
    16741 ctttactatt ccttgtgaga ttgaaaatga tgtcaactgt gcaggtcttg ctgaggcagt
    16801 atctggttca ggcaagggag caagtgtgac actttgcttg accattggaa ccggtatcgg
    16861 tggttgcttg attatggata ggaaagtctt ccatggtttt agcaattcag cctgtgaagt
    16921 cgggtatatg catatgcagg atggagcttt tcaggatctc gcttctacaa cagctttagt
    16981 gaaatatgta gctgaagccc atggagaaga tgttgatcag tggaatggcc gacgtatctt
    17041 taaggaagct actgaaggaa acaaaatctg catggaaggt attgaccgta tggttgacta
    17101 tctaggaaaa ggtctggcaa atatttgtta tgtggccaat ccagaagtag ttattcttgg
    17161 tggtggtatc atggggcaag aggctatcct caaacctaag atccgcacag ccttgaaaga
    17221 ggcattggta ccaagtttag cagaaaaaac acgattagaa tttgcccatc accaaaatac
    17281 agcagggatg ttgggtgcat attatcattt taagacaaaa caatcctagt ttggctcagc
    17341 caaactagga ttttcttaca cgtttttgtc tacgatagcc gttgagtttt ttattttccc
    17401 agtagctatt aaagattttt tccttgcttt cgcgattgat ttccaaaaag taggcataaa
    17461 tcaaatcgat aaagaagagc ataggaagtt gagcggatat tcgttggatg taggagggtt
    17521 ggctgtgggt ggctacaaga acagtctctg tataggtctg gctatcttta ttgggaacac
    17581 ttgtaaagag tacagtcttt gcccccatct ccttggcatc taatagacta tctaaaatag
    17641 aaggagttga gcctgaaagt gagaaaccca gtactagaca attttcatcc atgatgctgg
    17701 ttgtccaggc aaagccgtct tggtctgtca aagcttcgca gaccacacct agtcgcataa
    17761 aacgtaattt catttcacgg gcgacgaggc cagaactccc tgttccaaag aagtagatac
    17821 gctcagcatc ttcgattagc tgggcaattc gctctagttg gatttcgtca atcaagtctt
    17881 gtgtttgttc cctcatattg ctataacttc tgaggactcg tttggtcagt ggactatgct
    17941 tggagacttg gttggcttga ttttctgcct gatgttggta ttggaaaata aattctcggt
    18001 agccagtaaa gccacacttt ttagcaaagc gggtcaaagc agcttgagaa atatgtaatt
    18061 tttgggtgac ttgttgagaa gataaatcat ctgtaatcgt ttcagcttgc aaaaaatagc
    18121 gagcgatttc ttgctctagg tctgtcattt cttcaaaatg tgaatcaatg atagttgcga
    18181 tatctggttt gtccataggg aaagctcctt tacatgggtc atactggaag actagatcag
    18241 ggaatagtca cacttcatta taacacataa tataaggata gataaataaa aacgcatttc
    18301 tgttttaaaa acgaaaaaat cgaaaaagct tctttctttt ccataatttt ctactcaaat
    18361 tgtggtacaa ttaagagtaa gattttaagt tagaaatgag actgatttgt atgagaaaat
    18421 ttaacagcca ttcgattccg attcggctta atttattgtt ttcaatcgtc attttactct
    18481 ttatgaccat tattggtcgt ttgttgtata tgcaggtttt gaacaaggat ttttacgaaa
    18541 aaaagctagc ctcagctagt cagaccaaga ttacaagcag ttcagcccgt ggggaaattt
    18601 atgatgctag tggaaaacct ttggtagaaa atacgttaaa gcaggttgtt tcctttacgc
    18661 gtagcaataa aatgacggct acagacttaa aagaaacagc taaaaagtta ctgacttatg
    18721 tgagcatcag ttctccaaat ttgacagaac gccagctggc ggattactat ttggctgatc
    18781 ctgaaatcta taaaaaaaca gtggaagctc tcccaagtga gaaacgcttg gattcagatg
    18841 gcaatcgtct atccgaatca gaactgtata acaatgcggt cgatagtgtc caaacaagtc
    18901 aactaaacta tacagaggat gaaaagaaag aaatctatct ttttagtcag ttaaatgctg
    18961 ttggaaactt tgcgacagga accattgcta cagatcctct aaatgattct caggtggctg
    19021 ttattgcctc tatttcaaag gagatgcctg gcattagtat ttctacttct tgggatagaa
    19081 aggttttgga aacttccctt tcttctatag ttgggagtgt atccagtgaa aaagctggtc
    19141 tcccagcgga agaagcagaa gcctatctta aaaaaggcta ttctctaaat gatcgtgtag
    19201 gaacctccta tttggaaaag caatatgaag agaccttaca aggaaaacgc tcggtaaaag
    19261 aaatccatct ggataaatat ggcaatatgg aaagcgtgga tacaattgag gaaggtagta
    19321 agggaaacaa tatcaaactg accattgatt tggctttcca agatagcgtg gatgctttac
    19381 tgaaaagtta tttcaattcc gagctagaaa atggtggagc caagtattct gaaggtgtct
    19441 atgcagtcgc ccttaaccca aaaacaggtg cggttttgtc tatgtcaggg attaaacatg
    19501 acttgaaaac gggagagttg acgcctgatt ccttgggaac ggtaaccaat gtctttgttc
    19561 caggttcggt tgtcaaggcg gcgaccatca gctcaggttg ggaaaatgga gtcttgtcag
    19621 gaaaccagac cttgacagac cagtccattg tcttccaagg ttcagctccc atcaattctt
    19681 ggtatactca ggcttacggt tcattcccta tcacagcggt ccaagctctg gagtattcat
    19741 caaataccta tatggtccaa acagccttag gtcttatggg gcaaacctat caacccaata
    19801 tgtttgtcgg caccagcaat ctagagtctg ctatggagaa actgcgttca acctttggcg
    19861 aatatggctt gggtactgcg acaggaattg acctaccaga tgaatctact ggatttgttc
    19921 ccaaagagta tagctttgct aattacatta ctaatgcctt tgggcagttt gataactata
    19981 cgccgatgca gttggctcag tatgtagcaa ctattgcaaa taatggtgtt cgtgtggctc
    20041 ctcgtattgt tgaaggcatt tatggtaata atgataaggg aggactgggt gacttgattc
    20101 agcaactgca accgacagag atgaataagg tcaatatatc cgactccgat atgagcatct
    20161 tgcaccaagg tttttatcag gttgcccatg gtactagtga attgacaact ggacgtgcct
    20221 tttcaaatgg cgccttggta tccattagcg gaaaaacagg tacagccgaa agctatgtgg
    20281 cagatggtca gcaagcaacc aataccaatg cggtggccta tgccccatct gataatcccc
    20341 aaatcgctgt tgcagtggtc tttcctcata ataccaatct aacaaatggt gtaggacctt
    20401 ccattgcgcg tgacattatc aatctgtatc aaaaatacca tccaatgaac tagaaaggaa
    20461 attatgcttt atccaacacc tattgccaag ttgattgaca gttattctaa gttaccaggt
    20521 atcgggatta agacggctac gcgtctggcc ttttatacga ttgggatgtc tgctgatgat
    20581 gtcaatgaat ttgcaaaaaa tctcctttct gctaagagag aattgacata ttgttctatt
    20641 tgtggacgtt tgacagacga cgatccttgt tctatctgta ctgatccgac tcgtgaccag
    20701 acaacaattt tagttcttga ggatagtaga gatgtggcag ccatggaaaa tatccaagaa
    20761 taccatggac tctatcatgt ccttcatggc ctcatttctc ctatgaatgg tatcagtccg
    20821 gacgatatca atctcaagag ccttatgact cgtcttatgg atagtgaagt ttcagaagtg
    20881 attgtggcga ctaatgctac agcggatggt gaagcgactt ccatgtatct ttcacgtttg
    20941 ctcaagccgg ctggtatcaa ggttacgcgt ctagcacgag gtctcgctgt gggagcggac
    21001 attgagtatg cggacgaagt gacactctta cgagcgattg aaaatcggac agagttgtaa
    21061 gtgtaggcaa atttacgaac tccattcatt tataaaaaat caaagaggct gaaaatcgtt
    21121 cctatcggcc tctttttgta tagtgtgatg agtaggatca ggttcaagtt ttaaaaaacc
    21181 aagcaaatat gatatactaa agagcgagta ttctagtaga attaggacaa ataatatgaa
    21241 acaaacgatt attcttttat atggtggacg gagtgcggaa cgcgaagtct ctgtcctttc
    21301 agctgagagt gtcatgcgtg cggtcaatta cgaccgtttc acagtcaaga ctttctttat
    21361 cagtcagtca ggtgacttta tcaaaacaca ggaatttagt catgctccgg ggcaagaaga
    21421 ccgtctcatg accaatgaaa ccattgattg ggataagaaa gttgcaccaa gtgctatcta
    21481 cgaagaaggt gcagtggtct ttccagtcct tcacgggcca atgggagaag atggctctgt
    21541 tcaaggattc ttggaagttt tgaaaatgcc ttacgttggt tgcaacattt tgtcatcaag
    21601 tcttgccatg gataaaatca cgactaagcg tgttctggaa tctgctggta ttgcccaagt
    21661 tccttatgtg gctatcgttg aaggcgatga tgtgactgct aaaatcgctg aagtggaaga
    21721 aaaattggct tatccagtct tcactaagcc gtcaaacatg gggtctagtg tcggtatttc
    21781 taagtctgaa aaccaagaag aactccgtca agccttaaaa cttgccttcc gatatgacag
    21841 ccgtgtcttg gttgagcaag gagtgaatgc ccgtgaaatt gaggttggcc tcttgggtaa
    21901 ctacgatgtc aagagcacgc tacctggaga agttgtcaag gacgttgcct tttatgacta
    21961 cgatgccaag tatattgata acaagattac tatggatatt cctgccaaaa tcagtgatga
    22021 tgtggtggct gtcatgcgtc aaaatgcaga aacagccttc cgtgccattg gtggccttgg
    22081 tctatctcgt tgcgatttct tctatacaga taagggagag atttttctca acgagctcaa
    22141 taccatgcca ggttttaccc agtggtctat gtacccacta ctttgggaca atatggggat
    22201 cagctaccca gaactaatcg agcgtttggt tgaccttgcc aaggaaagtt ttgacaagcg
    22261 cgaagcgcat ttgatataaa atgaaagaga gggtagaagc cagaaccatc actgcacggt
    22321 gactagagtt ctcggacttc agcccttttt aaaggagtag aaatgaaatt aacaatccat
    22381 gaaattgccc aagttgttgg agccaaaaat gatatcagta tctttgagga cacccagtta
    22441 gaaaaagctg agtttgatag tcgtttgatt ggaactggag atttatttgt gccacttaaa
    22501 ggtgcgcgtg atggccatga ctttattgaa acagcctttg aaaatggtgc agcagtaacc
    22561 ttgtctgaga aagaggtctc aaatcatcct tacattctag tagatgatgt tttggcagcc
    22621 tttcaatccc tagcatccta ctatcttgaa aaaacgactg ttgatgtctt tgctgttaca
    22681 ggttcaaatg gcaagacaac gactaaggat atgttggcgc atttactgtc aacaagatac
    22741 aagacctaca aaacacaagg caattacaat aatgagattg gccttcctta cacagttctt
    22801 catatgcctg aaggaacaga aaagttggtt ttggagatgg gacaggatca cttgggagat
    22861 attcatctct tgtctgaatt ggctcgtcca aaaacagcca tcgtgacctt ggttggagaa
    22921 gctcatttgg cctttttcaa agaccgttct gagattgcta aaggaaaaat gcaaattgca
    22981 gacggaatgg cttcaggttc cttgctttta gcgccggctg accctatcgt agaggactat
    23041 ttgccaactg ataaaaaggt ggttcgtttt ggtcaaggag cagagctggg aattaccgac
    23101 ttggttgagc gcaaagatag tctgaccttc aaggtcaatt tcttagagca agcccttgat
    23161 ttgccagtaa ctggcaagta caatgcgaca aatgctatga ttgcatccta tgttgccttg
    23221 caagaaggag tttcagagga gcaaattcgt ttggccttcc aacatcttga attgacgcgt
    23281 aaccgtaccg agtggaagaa agcagccaat ggagcagata tcctgtcaga tgtttataat
    23341 gccaatccaa ctgctatgaa actgatttta gagactttct ctgccattcc agccaatgaa
    23401 ggtggcaaga aaattgcagt gttggcggat atgaaggagc ttggtgacca gtctgttcaa
    23461 cttcataatc agatgatttt gagcctctcc ccagatgtgc ttgatatcgt tattttctat
    23521 ggagaagata ttgctcagtt ggctcaattg gccagtcaaa tgtttccaat cggccacgtt
    23581 tactacttca agaaaacaga agaccaggat caatttgaag acctagtcaa gcaggttaag
    23641 gaaagccttg gagctcatga ccaaatcctg ctcaaaggct ctaactctat gaatctagcc
    23701 aagttggtag aaagtttaga aaatgaagac aagtgatttt gtcaagtatt tgcaaagaat
    23761 gattgccatt acagatactg gcttaacctt tacaaaagat ccgtttgacc gtgagcgcta
    23821 cgaagacttg cgaagtctgt tatctgaaat gttgaatcag gtatcagacc tggatgcaga
    23881 agaagtagca gaagtcttga aaccaacttc agcttatgcg actcccttaa tggacgtccg
    23941 tgcttggatt gttgaggatg agaagatttg tctggttagg ggacaaggag aggatagttg
    24001 ggctttgcca ggtggctttg gtgaagtcgg ctattctcca actgaaaata ttctcaagga
    24061 aattgaagaa gaaaccggtt ttaaagccaa agttgaaaga ctgctagctg tttttgatac
    24121 caatcgtttc caactacaga gtaaacaata tgcaaagttt gtttttgaat gtaagtttct
    24181 cgatggacaa ttccaagaaa atcaagaaat tgcagatctt caattttttg ccattgacca
    24241 actgccgaac ttatctgaaa aacgcattac caaggagcaa atagagcttc tttggcaggt
    24301 ttatcaaggt catagggggc aatatcttga ctaagaagat gattatcgta tttttaaatc
    24361 catttttaac aactagcatg gtataataat atgcaggaaa attttgaatt atgaggaaga
    24421 ctagatgaat ttatgggata ttttctttac gactcaggca accgagccgc ccaaatttga
    24481 ccttttttgg tatgttagcc tatttacgct cttagcctta accttttata cagcccatcg
    24541 ctatcgtgaa aagaaggttt accaacgatt tttccaaatc ttgcagactg ttcagttaat
    24601 ccttctttat ggttggtact gggtcaatca tatgccactg tcagaaagcc taccctttta
    24661 ccattgccgt atggctatgt ttgtggtact cttgcttcct ggtcagtcca aatataaaca
    24721 atactttgca ttattgggaa catttgggac attagcagcc tttgtttatc cagtgccaga
    24781 tgcttaccct tttccacata tcaccattct atcctttatc tttggtcatt tagcactctt
    24841 ggggaactct ctagtttatc tattgagaca gtataatgcg cgattgctgg atgtgaaggg
    24901 aatttttctc atgacctttg ccctaaatgc cttgattttt gtggtcaatt tggtgacagg
    24961 tggcgattac ggatttttga caaaaccgcc attggttggg gatcacggtc tagtagctaa
    25021 ttatttactt gtttcaattg tgctggtagc tactatcagt ttgactaaga aaatcttaga
    25081 attcttttta gctcaagaag cagaaaaaat gattgcaaag gaagcttaac acagagcttt
    25141 cttttttgct cttagagagt ttttacaagc agcttataaa ataagaattt ctgaatagac
    25201 aaactcaaaa aatggctggg aaatttagga aaaaagcaag cacgattaaa ttttttgtgt
    25261 tataatattt tgtgaatagc tatgcctatg tttagctatg gaataatacg aagtgcgaaa
    25321 cttggaagat agagaggaag cgatgtaatg gctagagaag gcttttttac aggtctagat
    25381 attggaacaa gctctgtcaa ggtgcttgtg gccgagcaga gaaatggtga attaaatgta
    25441 attggcgtga gtaatgccaa aagtaaaggt gtaaaggatg gaattattgt tgatattgat
    25501 gcagcagcaa ctgctatcaa gtcagccatt tcccaagcgg aagaaaaggc aggcatttcg
    25561 attaaatcag tgaatgtcgg cttgcctggt aatcttttgc aggtagaacc aactcagggg
    25621 atgattccag taacatctga tactaaggaa attacggatc aagatgttga aaatgttgtc
    25681 aaatcagctt tgacaaagag tatgacacct gaccgtgaag tcattacctt tattcctgaa
    25741 gaatttattg tggatggttt ccaagggatt cgtgacccac gtggtatgat gggggttcgc
    25801 cttgaaatgc gtggtttgct ttatacagga cctcgtacta tcttgcacaa tttgcgtaag
    25861 acggttgagc gtgcaggtgt tcaggttgaa aatgttatca tttcaccact agcaatggtt
    25921 cagtctgttt tgaacgaagg ggaacgtgaa tttggtgcta cagtgattga tatgggggca
    25981 ggtcaaacga ctgtcgctac aatccgtaat caagaactcc agttcacaca tattctccaa
    26041 gaaggtggag attatgtaac taaagatatc tccaaggttt tgaaaacctc tcgcaaatta
    26101 gcggaaggct tgaaactgaa ttacggggaa gcctatccgc ctcttgcaag caaagaaacc
    26161 ttccaagtag aggttattgg agaagtggaa gcagtcgaag tgacggaagc ctacttgtca
    26221 gaaattattt ctgcacgaat caagcacatc cttgaacaaa tcaagcaaga attagataga
    26281 aggcgtctat tggacctccc tggtggtatt gtcttaatcg gtgggaatgc cattttacca
    26341 ggtatggttg agcttgctca ggaagtcttt ggcgtccgtg tcaagcttta tgttccaaat
    26401 caagttggta tccgtaatcc agcctttgcg catgtgatta gtttatcaga atttgcgggt
    26461 caattaacag aagttaatct tttggctcag ggagcgataa aaggtgagaa tgacttaagt
    26521 catcagccaa ttagttttgg tgggatgctg caaaaaacag ctcagtttgt acaatcaacg
    26581 cctgttcaac cagctcctgc tccagaagta gagccggtgg cgcctacaga accaatggcg
    26641 gatttccaac aagcttcaca aaataaaccg aaattagcag atcgtttccg tggcttgatc
    26701 ggaagcatgt ttgacgaata aagaggaaaa ataaattatg acattttcat ttgatacagc
    26761 tgctgctcaa ggggcagtga ttaaagtaat tggtgtcggt ggaggtggtg gcaatgccat
    26821 caaccgtatg gtcgacgaag gtgttacagg cgtagaattt atcgcagcaa acacagatgt
    26881 acaagcattg agtagtacaa aagctgagac tgttattcag ttgggaccta aattgactcg
    26941 tggtttgggt gcaggaggtc aacctgaggt tggtcgtaaa gccgctgaag aaagcgaaga
    27001 aacactgacg gaagctatta gtggtgccga tatggtcttc atcactgctg gtatgggagg
    27061 aggctctgga actggagctg ctcctgttat tgctcgtatc gccaaagatt taggtgcgct
    27121 tacagttggt gttgtaacac gtccctttgg ttttgaagga agtaagcgtg gacaatttgc
    27181 tgtagaagga atcaatcaac ttcgtgagca tgtagacact ctattgatta tctcaaacaa
    27241 caatttgctt gaaattgttg ataagaaaac accgcttttg gaggctctta gcgaagcgga
    27301 taacgttctt cgtcaaggtg ttcaagggat taccgatttg attaccaatc caggattgat
    27361 taaccttgac tttgccgatg tgaaaacggt aatggcaaac aaagggaatg ctcttatggg
    27421 tattggtatc ggtagtggag aagaacgtgt ggtagaagcg gcacgtaagg caatctattc
    27481 accacttctt gaaacaacta ttgacggtgc tgaggatgtt atcgtcaacg ttactggtgg
    27541 tcttgactta accttgattg aggcagaaga ggcttcacaa attgtgaacc aggcagcagg
    27601 tcaaggagtg aacatctggc tcggtacttc aattgatgaa agtatgcgtg atgaaattcg
    27661 tgtaacagtt gtcgcaacgg gtgttcgtca agaccgcgta gaaaaggttg tggctccaca
    27721 agctagaact gctactaact accgtgagac agtgaaacca gctcattcac atggctttga
    27781 tcgtcatttt gatatggcag aaacagttga attgccaaaa caaaatccac gtcgtttgga
    27841 accaactcag gcatctgctt ttggtgattg ggatcttcgc cgtgaatcga ttgttcgtac
    27901 aacagattca gtcgtttctc cagtcgagcg ctttgaagcc ccaatttcac aagatgaaga
    27961 tgaattggat acacctccat ttttcaaaaa tcgttaagta aatgaatgta aaagaaaata
    28021 cagaacttgt ttttcgagaa gttgcagagg ctagtctgag tgctcatcga gagagtggtt
    28081 cggtctctgt cattgcagtt accaagtatg tagatgtacc gacagcggaa gccttgcttc
    28141 cgctaggtgt tcatcatatc ggtgaaaatc gtgtagataa gtttctggaa aaatatgaag
    28201 ctttaaaaga tcgagatgtg acttggcatt tgattggtac cttgcaaaga cgtaaggtga
    28261 aagatatcat tcaatacgtt gattatttcc atgcattgga ctcagtaaag ctagcagggg
    28321 aaattcaaaa aagaagtgac cgagtcatca agtgtttcct tcaagtaaat atttctaaag
    28381 aagaaagcaa acacggtttt tcgagagagg aactgctgga aatcttgcca gagttagcca
    28441 gactagataa gattgaatat gttggtttaa tgacgatggc accttttgag gctagcagtg
    28501 agcagttgaa agagattttc aaggcggccc aagatttaca aagagaaatt caagagaaac
    28561 aaattccaaa tatgcctatg accgagttaa gtatgggaat gagtcgtgat tataaagaag
    28621 cgattcaatt cggttccact tttgttcgta taggtacatc attttttaag taggagagaa
    28681 ccatgtcttt aaaagataga ttcgatagat ttatagatta ttttacggag gatgaggatt
    28741 caagtctccc ttatgaaaaa agagatgagc ctgtgtttac tccagtaaat tcttcacagg
    28801 aaccggctct cccaatgaat caaccttcac agtcggctgg tacaaaagag aacaatatca
    28861 ccagacttca tgcaagacaa caggaattgg caaatcagag tcagcgtgca acggataagg
    28921 tcattataga tgttcgttat cctagaaaat atgaggatgc aacagaaatt gttgatttat
    28981 tggcaggaaa cgaaagtatc ttgattgatt ttcagtatat gacagaggtg caggctcgtc
    29041 gttgtttgga ctatttggat ggagcttgtc atgttttagc tggaaatttg aaaaaggtag
    29101 cttctaccat gtatttgttg acaccagtga atgttattgt aaatgttgaa gatatccgtt
    29161 taccagatga agatcaacag ggtgagttcg gttttgatat gaagcgaaat agagtacgat
    29221 aatgattttt ttaattcgta tgatttataa tgcagtggat atttactccc tgattttggt
    29281 agccttcgct gtcatgtctt ggtttccagg tgcctacgaa tccagtttag gtcgttggat
    29341 tgtagcgttg gtgaaaccag tgcttgctcc cttgcaacgc ctgcctttac agatagcggg
    29401 tcttgattta tctgtttggg tcgcgattgt tttggttcga tttttaggag aaaacctagt
    29461 gcgttttctg gcgatgatag gatgaataaa gggatttatc agcatttctc catagaagat
    29521 cgtccatttc ttgacaaggg aatggaatgg ataaagaagg tagaagatag ctatgctcct
    29581 tttttaactc cttttatcaa tcctcatcag gagaagctat taaagatttt ggccaaaacc
    29641 tatggtcttg cttgtagcag tagtggggaa ttcgtctcga gtgagtatgt tcgagtttta
    29701 ttatacccag attatttcca accagagttt tcagattttg aaatatctct ccaggaaatt
    29761 gtgtattcca ataaatttga acatttaacg catgctaaga ttttagggac agtcatcaat
    29821 caattaggga ttgaacggaa actttttgga gatatcctag tagatgaaga acgggcgcag
    29881 attatgatta atcagcagtt tcttcttctc tttcaagatg gactaaagaa aattggtcgt
    29941 atacctgttt cgctggagga acgtcctttc accgagaaaa tagataagct agaacagtat
    30001 cgagaactgg atttatctgt gtttagtttt cgattagatg ttcttttatc aaatgttttg
    30061 aaactatcta ggaatcaagc aaaccagttg attgaaaaga aacttgtcca agtaaattat
    30121 catgtggtag acaaatcaga ttacactgtt caagttggag acttgattag tgtgagaaaa
    30181 tttggccgct tgagattact tcaagataag ggacaaacga aaaaagagaa gaaaaaaata
    30241 accgtccagt tattattaag taagtgagga atagaatgcc aattacatca ttagaaataa
    30301 aggacaagac ttttggaact cgattcagag gttttgatcc agaagaagtc gatgaatttt
    30361 tagatattgt ggttcgtgat tacgaagatc ttgtgcgtgc gaatcatgat aaaaatttgc
    30421 gtattaagag tttagaagag cgtttgtctt actttgatga aataaaagat tcattgagcc
    30481 agtctgtatt gattgctcag gatacagctg agagagtgaa acaggcggcg catgaacgtt
    30541 caaacaatat cattcatcaa gcagagcaag atgcgcaacg cttgttggaa gaagctaaat
    30601 ataaggcaaa cgagattctt cgtcaagcaa ctgataatgc taagaaagtc gctgttgaaa
    30661 cagaagaatt gaagaacaag agccgtgtct tccaccaacg tctcaaatct acaattgaga
    30721 gtcagttggc tattgttgaa tcttcagatt gggaagatat tctccgtcca acagctactt
    30781 atcttcaaac cagtgatgaa gcctttaaag aagtggttag cgaagtactt ggagaaccga
    30841 ttccagctcc aattgaagaa gaaccaattg atatgacacg tcagttctct caagcagaaa
    30901 tggaagaatt acaagctcgt attgaggtag ccgataaaga attgtctgaa tttgaagctc
    30961 agattaaaca ggaagtggaa actccaactc ctgtagtgag tcctcaagtt gaagaagagc
    31021 ctctgctcat ccagttggcc caatgtatga agaaccagaa gtagctccag tgcatccgac
    31081 aggtccaaca ccagctacag aaactgttga ttcagcaccg ggatttgaag caccgcaaga
    31141 atctgttaca attttataag aaatattctg agaacaatat cttatcctta tattcccagc
    31201 gagcaggaga tggtgtgagt cctgcattcc ctatcgataa gattatcctc tcaaaaactc
    31261 aagtctgaag ctagtaagat ttgacgttcc ccacgttacg ggataagagg gagaaagact
    31321 aaaacttttt ccgaataaag gtggtaccac gattttcgtc ctttttggaa gtcgtggttt
    31381 ttaatttgtt attatttata aaggagatac catgaaactc aaagacaccc ttaatcttgg
    31441 gaaaactgaa ttcccaatgc gtgcaggcct tcctaccaaa gagccagttt ggcaaaagga
    31501 atgggaagat gcaaaacttt atcaacgtcg tcaagaattg aaccaaggaa aacctcattt
    31561 caccttgcat gatggccctc catacgctaa cggaaatatc cacgttggac atgctatgaa
    31621 caagatttca aaagatatca ttgttcgttc taagtctatg tcaggatttt acgcaccatt
    31681 tattcctggt tgggatactc atggtctgcc aatcgagcaa gtattgtcaa aacaaggtgt
    31741 caaacgtaaa gaaatggact tggttgagta cttgaaactt tgccgtgagt acgctctttc
    31801 tcaagtagat aaacaacgtg aagattttaa acgtttgggt gtttctggtg actgggaaaa
    31861 tccatatgtg accttgactc ctgactatga agcagctcaa attcgtgtat ttggtgagat
    31921 ggctaataag ggttatatct accgtggtgc caagccagtt tactggtcat ggtcatctga
    31981 gtcagccctt gctgaagcag agattgaata ccatgatttg gtttcaactt ccctttacta
    32041 tgccaacaag gtaaaagatg gcaaaggagt tctagataca gatacttata tcgttgtctg
    32101 gacaacgact ccatttacca tcacagcttc tcgtggtttg acggttggtg cagatattga
    32161 ttacgttttg gttcaacctg ctggtgaagc tcgtaagttt gtcgttgctg ctgaattatt
    32221 gactagcttg tctgagaaat ttggctgggc tgatgttcaa gttttggaaa cttaccgtgg
    32281 ccaagaactc aaccacatcg taacagaaca cccatgggat acagctgtag aagagttggt
    32341 aattcttggt gaccacgtta cgactgactc tggtacaggt attgtccata cagcccctgg
    32401 ttttggtgag gacgattaca atgttggtat tgctaataat cttgaagtcg cagtgactgt
    32461 tgatgaacgt ggtatcatga tgaagaatgc tggtcctgaa tttgaaggtc aattctatga
    32521 aaaggtagtt ccaactgtta ttgaaaaact tggtaacctc cttcttgccc aagaagaaat
    32581 ctctcactca tatccatttg actggcgtac taagaaacca atcatctggc gtgcagttcc
    32641 acaatggttt gcctcagttt ctaaattccg tcaagaaatc ttggacgaaa ttgaaaaagt
    32701 gaaattccac tcagaatggg gtaaagtccg tctttacaat atgatccgtg accgtggtga
    32761 ctgggttatc tctcgtcaac gtgcttgggg tgttccactt cctatcttct acgctgaaga
    32821 tggtacagct atcatggtag ctgaaactat tgaacacgta gctcaacttt ttgaggaaca
    32881 tggttcaagc atttggtggg aacgtgatgc caaagacctc ttgccagaag gatttactca
    32941 tccaggttca ccaaacggcg agttcaaaaa agaaactgat atcatggacg tttggtttga
    33001 ctcaggttca tcatggaatg gagtggtggt aaaccgtcct gaattgactt atccagctga
    33061 cctttacctt gaaggttctg accaataccg tggttggttc aactcatcac tcattacatc
    33121 tgttgccaac catggcgtag caccttacaa acaaatcttg tcacaaggtt ttacccttga
    33181 tggtaaaggt gagaagatgt ctaaatcact tggaaatacc attgctccaa gtgatgttga
    33241 aaaacaattt ggtgcagaaa tcttgcgtct ctgggtaaca agtgttgact caagcaatga
    33301 cgtgcgtatc tctatggata ttttgagcca agtttctgaa acttaccgta agattcgtaa
    33361 cactcttcgt ttcttgattg ccaatacatc tgactttaac ccagctcaag atgtagtcgc
    33421 ttacgatgaa cttcgttcag ttgataagta catgactatt cgctttaacc agcttgtcaa
    33481 gaccattcgt gatgcctatg cggactttga attcttgaca atctacaagg ccttggtgaa
    33541 ctttatcaac gttgacttgt cagccttcta ccttgatttt gccaaagatg ttgtttacat
    33601 tgaaggtgcc aaatcattgg aacgccgtca aatgcagact gttttctatg acattcttgt
    33661 caaaatcacc aaactcttga caccaatcct tcctcacact gcggaagaaa tctggtcata
    33721 tcttgagttt gaagctgaag atttcgttca attgtcagaa ttgccagaag ctcaaacttt
    33781 tgctaatcaa gaagaagtct tggatacatg ggcagccttc atggacttcc gtggacaagc
    33841 tcaaaaagcc ttggaagaag ctcgtaatgc aaaagttatc gggaaatcgc ttgaagcaca
    33901 cttgacagtt tatccaaatg aagttgtgaa aactctactc gaagcagtaa acagcaatgt
    33961 ggctcaactt ttgatcgtgt cagacttgac catcgcagaa gggccagctc cagaagctgc
    34021 tgttagcttc gaagatgtag ccttcacagt tgaacgcgct gcaggtcaag tatgtgaccg
    34081 ttgccgtcgt atcgacccaa caacagcaga acgcagctac caggcagtca tctgtgacca
    34141 ctgtgcaagc atcgtagaag aaaactttgc ggaagcagtc gcagaaggat ttgaagagaa
    34201 ataaggttga aaagtctagg caaaattcaa tttgagaaga aaagacaact aattttatag
    34261 tctattaaac gcattgtatc acgtttttga atacctgata tgatgcgttt tttatttatt
    34321 ttaaaaattt gcgaggtatg actttttata ctcaacaaga atcaaagaga aacttagcaa
    34381 gctaacagta gtaagataaa ataggaattt gatattaggg ataagattgc taaatagtgt
    34441 aatattttta caataataaa tttatatagt tatt
//
LOCUS       DAVMAF010000016        33403 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMAF010000016 DAVMAF010000000
VERSION     DAVMAF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.016"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            72..157
                     /locus_tag="VYO97_000743"
     tRNA            72..157
                     /locus_tag="VYO97_000743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Leu,seq:aag)
     gene            440..1570
                     /gene="ugpC"
                     /locus_tag="VYO97_000744"
     CDS             440..1570
                     /gene="ugpC"
                     /locus_tag="VYO97_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1753575.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(1717..1863)
                     /locus_tag="VYO97_000745"
     CDS             complement(1717..1863)
                     /locus_tag="VYO97_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753576.1"
                     /translation="MEIKEQTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            2071..2829
                     /locus_tag="VYO97_000746"
     CDS             2071..2829
                     /locus_tag="VYO97_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1753577.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            2916..3428
                     /locus_tag="VYO97_000747"
     CDS             2916..3428
                     /locus_tag="VYO97_000747"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1753578.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            3610..4554
                     /locus_tag="VYO97_000748"
     CDS             3610..4554
                     /locus_tag="VYO97_000748"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1753579.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            4563..5240
                     /locus_tag="VYO97_000749"
     CDS             4563..5240
                     /locus_tag="VYO97_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1753580.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            5338..6096
                     /locus_tag="VYO97_000750"
     CDS             5338..6096
                     /locus_tag="VYO97_000750"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1753581.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            6134..7638
                     /gene="lytC"
                     /locus_tag="VYO97_000751"
                     /pseudo
     CDS             6134..7638
                     /gene="lytC"
                     /locus_tag="VYO97_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
     gene            8144..8662
                     /locus_tag="VYO97_000752"
     CDS             8144..8662
                     /locus_tag="VYO97_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1753582.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            8792..9298
                     /locus_tag="VYO97_000753"
     CDS             8792..9298
                     /locus_tag="VYO97_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1753583.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAEFQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            9298..9468
                     /locus_tag="VYO97_000754"
     CDS             9298..9468
                     /locus_tag="VYO97_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753584.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAVYMTLF"
     gene            9500..10732
                     /gene="clpX"
                     /locus_tag="VYO97_000755"
     CDS             9500..10732
                     /gene="clpX"
                     /locus_tag="VYO97_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1753585.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            10741..11328
                     /locus_tag="VYO97_000756"
     CDS             10741..11328
                     /locus_tag="VYO97_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1753586.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            11339..11719
                     /locus_tag="VYO97_000757"
     CDS             11339..11719
                     /locus_tag="VYO97_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1753587.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            11771..12661
                     /gene="rapZ"
                     /locus_tag="VYO97_000758"
     CDS             11771..12661
                     /gene="rapZ"
                     /locus_tag="VYO97_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1753588.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            12658..13635
                     /locus_tag="VYO97_000759"
     CDS             12658..13635
                     /locus_tag="VYO97_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1753589.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVVGESHIVDHRGIVDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            13632..14543
                     /gene="whiA"
                     /locus_tag="VYO97_000760"
     CDS             13632..14543
                     /gene="whiA"
                     /locus_tag="VYO97_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1753590.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(14631..15757)
                     /locus_tag="VYO97_000761"
                     /pseudo
     CDS             complement(14631..15757)
                     /locus_tag="VYO97_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15899..16279)
                     /locus_tag="VYO97_000762"
     CDS             complement(15899..16279)
                     /locus_tag="VYO97_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1753591.1"
                     /translation="MTIEELVDSHGVTLAYFDNELWQRPGVYIKEISIIFINRELSEN
                     AKKRVIYHELGHLEHSTALYKNNHTRCENEANRHMIHKLLEEELALSDDHQSFNYIQF
                     MQKHELRTVTDELMVIDEYYELIG"
     gene            complement(16300..16662)
                     /locus_tag="VYO97_000763"
     CDS             complement(16300..16662)
                     /locus_tag="VYO97_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753592.1"
                     /translation="MLSEENIMSQDTYSKSEIDLKLDKINSDTQHGFEKIDLKVDQLR
                     QEMRSGFEKIDLKFEQVDLKFDNFEKRVETMFLTQENKRLEEQAKSKKEFMYWFIGLL
                     VSTLLGILAIIVTILTTK"
     gene            complement(16783..17205)
                     /locus_tag="VYO97_000764"
     CDS             complement(16783..17205)
                     /locus_tag="VYO97_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753593.1"
                     /translation="MTNIPMKKNPFREKMTAISIVNPETAQLLGTITNFDMFPGSTSL
                     VAFLDFFNLRPETDYILSLTAHFPNGTSYPVHATRINIARQEFVLLEDGFGMATGNFS
                     FNFTIEKPSDFYFFFVLMDENGQEVDTAYSYHHFGKWG"
     gene            complement(17207..17563)
                     /locus_tag="VYO97_000765"
     CDS             complement(17207..17563)
                     /locus_tag="VYO97_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753594.1"
                     /translation="MFETFEKIKSLAKKQGISLNTLEDRVGLGKNYIYSLKNKKTPSA
                     EHISKIADYFNVSTDYLLGRTDNPAIAGDDTIAGYTSDDLRKMAENAKTFDGKPLTEE
                     DIDAIQNIIEIYLRGR"
     gene            complement(17980..18219)
                     /locus_tag="VYO97_000766"
     CDS             complement(17980..18219)
                     /locus_tag="VYO97_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753595.1"
                     /translation="MTFKEYLLKASKRDIYDDGKDFDFETIFAREILRYAHDSELETK
                     TGFFRHLEIMNADSWFVELARSIYQDFEKSISDSH"
     gene            18284..18475
                     /locus_tag="VYO97_000767"
     CDS             18284..18475
                     /locus_tag="VYO97_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1753596.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            complement(18589..18810)
                     /locus_tag="VYO97_000768"
     CDS             complement(18589..18810)
                     /locus_tag="VYO97_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753597.1"
                     /translation="MSKLTKEDVLQVSQDIINDAIPVIKDMLDEVFEKYPIDIEIREA
                     IFYSVLVAHKLSTETTVSLLTQLVNAQEN"
     gene            18928..19071
                     /locus_tag="VYO97_000769"
     CDS             18928..19071
                     /locus_tag="VYO97_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753598.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     RTV"
     gene            19151..19423
                     /locus_tag="VYO97_000770"
     CDS             19151..19423
                     /locus_tag="VYO97_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753599.1"
                     /translation="MEAVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            19420..19614
                     /locus_tag="VYO97_000771"
     CDS             19420..19614
                     /locus_tag="VYO97_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753600.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEK"
     gene            19611..19802
                     /locus_tag="VYO97_000772"
     CDS             19611..19802
                     /locus_tag="VYO97_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753601.1"
                     /translation="MMEYIYLVIIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMN
                     LRNSDKQFTYDVEPPEGLK"
     gene            19814..20155
                     /locus_tag="VYO97_000773"
     CDS             19814..20155
                     /locus_tag="VYO97_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753602.1"
                     /translation="MTQAEQIREYYREHPAASCDEVAEVVGTTKSNVSANLAKDIKAG
                     RCVRLEDKSYDYSPYYNHTQALTELVDWKNDTRREWVDMLTRAAEKETVSNVMRLLIK
                     EANKLMKEVTK"
     gene            20156..20386
                     /locus_tag="VYO97_000774"
     CDS             20156..20386
                     /locus_tag="VYO97_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753603.1"
                     /translation="MVRNKLTDLTNTLFAQLETLDDRDLTADELKTELQRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            20400..21122
                     /locus_tag="VYO97_000775"
     CDS             20400..21122
                     /locus_tag="VYO97_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1753604.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTTYTKTQIGEVRRRLGLPVGKV
                     YQGKLLTKEQHDYLVSIQKNKISRDVANEMNLKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNSGQFKKGNRPLNYVPVGTINYTTDGYPKEKIGEPNQW
                     VLKHRKVWEEHHGPIPKGHSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            21115..21597
                     /locus_tag="VYO97_000776"
     CDS             21115..21597
                     /locus_tag="VYO97_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEV1753605.1"
                     /translation="MASLYELTGQFLTVYQMDIDDETKTDTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELVRTEKLVIK
                     "
     gene            21597..21956
                     /locus_tag="VYO97_000777"
     CDS             21597..21956
                     /locus_tag="VYO97_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753606.1"
                     /translation="MELMNKTRVTDSLAVVIGPESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            21956..22441
                     /locus_tag="VYO97_000778"
     CDS             21956..22441
                     /locus_tag="VYO97_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753607.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            22404..23597
                     /locus_tag="VYO97_000779"
     CDS             22404..23597
                     /locus_tag="VYO97_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEV1753608.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            23616..23933
                     /locus_tag="VYO97_000780"
     CDS             23616..23933
                     /locus_tag="VYO97_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753609.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            23933..24760
                     /locus_tag="VYO97_000781"
     CDS             23933..24760
                     /locus_tag="VYO97_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1753610.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYADQ
                     VDDKAILKILATIRGAVEPAKGKLAPKKEAAKTTKPAKTEKTKEAPKKEVDSDDELF"
     gene            24780..25286
                     /locus_tag="VYO97_000782"
     CDS             24780..25286
                     /locus_tag="VYO97_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753611.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKNLDWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALKRKAVGSYYNLVISVTKGKDGREFRNYDLEEEEAQPLTEAD
                     IDDDDLPF"
     gene            25459..26280
                     /locus_tag="VYO97_000783"
     CDS             25459..26280
                     /locus_tag="VYO97_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEV1753612.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIDFADKPAMTPSEIE
                     NFWDGYPNANIALKTTNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKDLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPNPLPEKEMMRTIESMIKKDRR"
     gene            26283..27626
                     /locus_tag="VYO97_000784"
     CDS             26283..27626
                     /locus_tag="VYO97_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1753613.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSSQAMLDGMET
                     FFSERSYNPVIEYMEKAAGKWDGRKRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQTEYLKDKTGER
                     RFLPVMADSKRQKKHPMEIEPETIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKDSEKTNR"
     gene            27920..28072
                     /locus_tag="VYO97_000785"
     CDS             27920..28072
                     /locus_tag="VYO97_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753614.1"
                     /translation="MSYTVTLYFDNMVDETHLFKKKGDAAKCKAQLESKYRGDRMYKV
                     KMEEME"
     gene            28072..28410
                     /locus_tag="VYO97_000786"
     CDS             28072..28410
                     /locus_tag="VYO97_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753615.1"
                     /translation="MSYDLEILAKIESGDYICIAEPRYSSPTYNLGKMFRVAMDWDFD
                     QGTIYNVADIFENIKRGITELERQPEKYVQYEPANKWGTINDALYVLRSLRDCILEQD
                     IDTKYLYVRW"
     gene            28561..28875
                     /locus_tag="VYO97_000787"
     CDS             28561..28875
                     /locus_tag="VYO97_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753616.1"
                     /translation="MEELKQKVNAVYNWTVEDGKPQPPKQDLPQAVKDRVDYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPGMSQVVIAVYLIYR
                     GN"
     gene            28877..29383
                     /locus_tag="VYO97_000788"
     CDS             28877..29383
                     /locus_tag="VYO97_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1753617.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLGEPQPVK
                     VPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYEVEK
                     EKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCP
                     GIEIEEVE"
     gene            29386..29451
                     /locus_tag="VYO97_000789"
                     /pseudo
     CDS             29386..29451
                     /locus_tag="VYO97_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29444..29623
                     /locus_tag="VYO97_000790"
     CDS             29444..29623
                     /locus_tag="VYO97_000790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753618.1"
                     /translation="MNKRQRKKKILNGLNKEERYHRTHCPVCDSEAGLFDRYFNTYGF
                     CSEYCGYEYYGISRL"
     gene            29644..29958
                     /locus_tag="VYO97_000791"
     CDS             29644..29958
                     /locus_tag="VYO97_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753619.1"
                     /translation="MTLFDEVQQLSSESHAKWFERYFEKYNLEQKLKTSAQKGYTGYL
                     INVWSVRDEYLRNRLGDEITFEALRELLGAGFTVKYKLYLSKNIFTGQDFVSNKKIHI
                     TW"
     gene            30033..30494
                     /locus_tag="VYO97_000792"
     CDS             30033..30494
                     /locus_tag="VYO97_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1753620.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWSDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLQLGRMINRLKNPKHRTILRMTYINK
                     MYVDDICDSMRGISSPTYYRLKKQAVKELDIILSELIVNDSNCTGMKSKIC"
     gene            30519..30601
                     /locus_tag="VYO97_000793"
     tRNA            30519..30601
                     /locus_tag="VYO97_000793"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30550..30552,aa:Tyr,seq:gta)
     gene            30906..31346
                     /locus_tag="VYO97_000794"
     CDS             30906..31346
                     /locus_tag="VYO97_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1753621.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLSYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            31324..32622
                     /locus_tag="VYO97_000795"
     CDS             31324..32622
                     /locus_tag="VYO97_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1753622.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWGNLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            32634..>33403
                     /locus_tag="VYO97_000796"
     CDS             32634..>33403
                     /locus_tag="VYO97_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1753623.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEIFTENLHYHMVLRSTDSGTTQSEEAIPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVES"
BASE COUNT        10846 a         5728 c         7342 g         9487 t
ORIGIN      
        1 gaaaatgaaa aaatttgaaa aaaagtgttg acaagcatcc caaaagttga tatactagta
       61 aagtaatcga tgcggggatg gcggaattgg cagacgcgca ggactaagga tcctgtgacc
      121 gctttaggtc gtgagggttc aagtccctct ctccgcatag gaaaagagct agctaaagct
      181 agctcttttg tatttctcta aaaaattaga atccagtcaa atgtagtgga gaatcagcga
      241 aaatatggga aaactgatcc ttgtttctgc ttttgtcata gaagaataat aaagattatc
      301 ttcttcaaat tctccgatat tctctaaagt tttgtgcaag ttgcacagaa cttttttatt
      361 tttttggtca tcttgccata gaaatataaa gcgttttcat ctataatata attatcaaaa
      421 gacaaaagga gttcacctca tggtagaatt gaatcttaaa aatatttaca aaaaatatcc
      481 aaacagcgaa cactattcag ttgaagattt caacttgaac atcaaagata aagaatttat
      541 cgttttcgta ggaccttcag gatgtggtaa atcaactaca ctccgtatga ttgctggtct
      601 tgaagacatt acagaaggta ctgcatctat cgatggcgtg gttgtcaacg acgtagctcc
      661 aaaagaccgt gatatcgcca tggtattcca aaactacgct ctttacccac acatgactgt
      721 ttatgacaac atggctttcg gtttgaaatt gcgtaaatac agcaaagaag acattaacaa
      781 acgtgttcaa gaagcagctg aaatacttgg attgaaagaa ttcttggaac gtaaaccagc
      841 tgacctttca ggtggtcaac gtcaacgtgt tgccatgggg cgtgcgattg tccgtgatgc
      901 gaaagtattc ttgatggacg aacctttgtc aaacttggat gccaaacttc gtgtatcaat
      961 gcgtgctgaa atcgctaaaa ttcaccgtcg tatcggagct acaactatct atgtaactca
     1021 cgaccaaaca gaagcgatga cacttgcaga ccgtatcgtt attatgtcag ctactaagaa
     1081 ccctgctggt acaggtacta tcggacgtgt agaacaaatc ggtactcctc aagaagttta
     1141 caaaaatcca gttaacaaat tcgttgcagg attcatcgga agcccagcta tgaacttcat
     1201 caacgtgaaa ttggttggta gcgaaattgt ttctgacggt ttccgtttga aagtgccaga
     1261 aggagcattg aaagttcttc gtgaaaaagg ctacgaagga aaagaattga tctttggtat
     1321 ccgtccagaa gacgtgaatg cagaacctgc tttccttgaa acattcccag actgtgttgt
     1381 aaaagcgact atctctgtat cagaactgct tggttcagaa tctcaccttt actgccaagt
     1441 tggtaaagac gagtttgttg caaaagttga tgctcgtgac tacttgcaaa caggtgcaac
     1501 agttgagctt ggatttgact tgaacaaagc acacttcttc gatgtagaaa ctgaaaaaac
     1561 aatctactaa aataaataaa attcaaagca ctacaagaaa agatatctct ttatcaattg
     1621 tagtggagag atatcagtta atctagggag agaaacaaaa tgcttctctc ctttttgcta
     1681 gggaagtcat attatgcatc tatattgtga tgctctttaa tactcttcga aaatctcttc
     1741 aaaccacgtc aacgtcgcct tgccgtacgt atgattactg acttcgtcag ttttatctgc
     1801 aacctcaaag ctgtactttg agcagcttac ggctagtttc ctagtttgct ctttgatttc
     1861 cattgagtat tatttgtggg taccatctac aagtgaagct atatgcgtaa actacgtgag
     1921 caattgaatt cgaactagag aggtaataat aaatttatgc tatagttatg gtgacttgta
     1981 tgcttttgat tctagtttat caaataatag attagaattg tcagataata tcattttgtg
     2041 ttataatgaa gaaaaaacag aggtgttcaa atgtcagaag caggtcataa gtttttagca
     2101 aaattgggga aaaaacgctt acgtccaggt ggaaagcgtg ccacagattg gttaattgca
     2161 gaaggaggat tttcaaaaga aaagagaata ctagaggttg cgtgtaatag gggaactaca
     2221 gcaattgagt tggcacagcg ttttggttgc aagataactg ctgttgatat ggatgctcaa
     2281 gctttagaag tggctaaaaa atctgctgga acggcaggtg ttgctcattt aatcagtttt
     2341 gaaagagcaa atgcaatgaa acttccttat caagatgcta gttttgatat tgttataaat
     2401 gaagctatgc tgactatgca agccgatcaa gctaagaaaa aatgtgtaat ggaatatcta
     2461 agggtattaa aacctggagg tcttctcttg acacatgatg tgcttcttaa ggaagctaaa
     2521 gagtctatca gacaggaatt atcacaagca attcatgtaa atgtaggtcc tttaactcaa
     2581 gatggttggg aacaggtgat gatagaatca ggttattgtg atgtgaaagc attgactggt
     2641 gaaatgacat taatgaaatt atcgggtatg atttatgacg aaggtttgct aggaactttg
     2701 aaaatttgtg taaatgcttg taaaaaggag aatagaaagc agtttttaac tatgtataaa
     2761 atgtttgcta agaataaaca gaaattgggc tttattgcga tggctagtta taaatcgtca
     2821 aaacgttaga taattattga agttaacttt tccttttttc tttcttaaaa aatatgctat
     2881 aatagagagt aaaaaacttt gaaagaaaga aaaagatgaa tttaaaagat tacattgcaa
     2941 caattgaaaa ttatccaaag gaaggcatta ccttccgtga tattagtcct ttgatggctg
     3001 atggaaatgc ttatagctac gctgttcgtg aaatcgttca gtatgctact gacaagaaag
     3061 tcgacatgat cgtgggacct gaagctcgtg gatttatcgt gggttgtcca gttgcctttg
     3121 agttgggaat tggttttgcg cctgttcgta agccaggtaa attgccacgc gaagttattt
     3181 ctgctgacta tgaaaaagag tacggtgtcg atactttgac tatgcacgcg gatgccatta
     3241 agccaggtca acgtgttctt attgtagatg accttttggc gacaggtgga actgttaagg
     3301 caactatcga gatgattgaa aaacttggtg gtgttatggc aggttgtgcc ttccttgttg
     3361 aattggatga attgaacggc cgtgaaaaaa ttggtgacta cgactacaaa gttcttatgc
     3421 attattaatg aaaacagtcc ctagggctgt tttctctaca ctaggatata aaaatagact
     3481 ataactagtt agagaaaaac tataattgaa aactatatct tcttgcagta taataaaagg
     3541 actaagtgtt tgagatttgt cttcaaacat atgcaattat tcctgaaaga gtacagttag
     3601 gagagggtta tgccgattcg aattgataaa aaattgccag ctgttgagat tttacggaca
     3661 gagaatatct ttgtcatgga tgatcaacgt gctgcccacc aagatatccg tcctttgaag
     3721 attttaattt taaatctcat gccacagaaa atggtcacag agacccagtt gttgcgccac
     3781 ttggctaata cacccctaca actggatatt gattttctct atatggagag ccaccgttct
     3841 aaaacaactc gttcagagca catggagacc ttctataaaa cttttcctga agtcaaggat
     3901 gagtattttg atgggatgat catcacgggt gctccagttg agcatttacc atttgaggaa
     3961 gtggactatt gggaggaatt tagacagatg cttgagtggt ctaagactca tgtctattcg
     4021 acccttcata tctgttgggg ggctcaggct gggctttatc tgcgctatgg tgtagaaaaa
     4081 taccagatgg acagtaagct atcaggtatt tatcctcagg acaccctaaa agagggtcac
     4141 cttctattta gaggctttga tgatagctat gtatcccctc attcacggca cacggagatt
     4201 tctaaggaag aggtcttaaa caagaccaat ctcgagattt tatcagaagg acctcaggtt
     4261 ggggtttcta ttttggccag tcgtgattta cgagaaattt atagttttgg tcatttggag
     4321 tatgaccgtg ataccttggc aaaagagtat tttcgagatc gtgatgcagg tttcgaccca
     4381 catattccag aaaattactt taaagatgat gatgttaatc aggtaccttg tctttgttgg
     4441 tcttcatctg cagccctctt tttcagtaat tgggtaaacc atgcggtcta tcaggagacg
     4501 ccttttgact ggagaaagat agaagatgat gcatctgcat ataggtattt ataagaggaa
     4561 ttatgacata tttagacgct tttaaatcag gtaccttggt tttaccgagt gccctgctct
     4621 tgcattttaa ggaactcttt ccttctagcg acgattttct ggtttggcaa tttttctatt
     4681 tgcaaaatac gacaggctta gaagaaatat cgccaagcca gattgctgaa aggattggca
     4741 aggaaatttc ggatgtcaac cagtccattt ctaatctgac ggaaagggga ctgctccagt
     4801 atcgtactat cgaattaaat ggcgaaattg aattgctctt tgatgctagt ttggccttgg
     4861 aacgtttgga tgacctgttt ggagcagttc attcaagttc agaccagcta acaccacaaa
     4921 accagctcaa ggatttggtg gaaaccttcc agcaggagtt gggacgattg ttgacgcctt
     4981 ttgagattga ggatttgacc aagacactaa aggaagatgg aaccagtgct gacttgatta
     5041 aggaggctct tcgtgaagct gttttgaacg gaaaaccaaa ctggaagtac attcaggcga
     5101 ttttgagaaa ctggcgccat gaaggaatca agagtgtggc tcaaattgag gccaagagag
     5161 cagaaagaga agcaagcaat cctcagttga cacaggtatc tgcagatttc ataaatgcca
     5221 tggatctctg gaaggattaa tccatgcaag taggcttgaa atccgagtaa gatttgcaag
     5281 ctgtgtataa ttgtgataga ataaatagaa aataaattga aaaaagaggt atgtgaaatg
     5341 tcacgtaaac catttatcgc tggtaactgg aaaatgaaca aaaatccaga agaagctaaa
     5401 gcattcgttg aagcagttgc atcaaaactt ccttcatcag atcttgttga agcaggtatc
     5461 gctgctccag ctcttgattt gacaactgtt cttgctgttg caaaaggctc aaaccttaaa
     5521 gttgctgctc aaaactgcta ctttgaaaat gcaggtgctt tcactggtga aactagccca
     5581 caagttttga aagaaatcgg tactgactac gttgttatcg gtcactcaga acgccgtgac
     5641 tacttccatg aaactgatga agatatcaac aaaaaagcaa aagcaatctt tgcgaacggt
     5701 atgctcccaa tcatctgttg tggtgaatca cttgaaactt acgaagctgg taaagctgct
     5761 gaattcgtag gtgctcaagt atctgctgca ttggctggat tgactgctga acaagttgct
     5821 gcctcagtta tcgcttatga gccaatctgg gctatcggta ctggtaaatc agcttcacaa
     5881 gacgatgcac aaaaaatgtg taaagttgtt cgtgacgttg tagctgctga ctttggtcaa
     5941 gaagtcgcag acaaagttcg tgttcaatac ggtggttctg ttaaacctga aaatgttgct
     6001 tcatacatgg cttgcccaga cgttgacggt gcccttgtag gtggtgcgtc acttgaagct
     6061 gaaagcttct tggctttgct tgactttgta aaataatcag taagtagcaa aagctaggtg
     6121 gaacagcatt cagatgtctg ttacattttt tataggagag aaagattgaa aacaaaaatt
     6181 ggattagcaa gtatctgttt actaggcttg gcaactagtc atgtcgctgc aaatgaaact
     6241 gaagtagcaa aaacttcgca ggatacaacg acagcttcaa gtagttcaga gcaaaatcag
     6301 tcttctaata aaacgcaaac gagcgcagaa gtacagacta atgctgctgc ccactgggat
     6361 ggggattatt atgtaaagga tgatggttct aaagctcaaa gtgaatggat ttttgacaac
     6421 tactataagg cttggtttta tattaattca gatggtcgtt actcgcagaa tgaatggcat
     6481 ggaaattact acctgaaatc aggtggatat atggcccaaa acgagtggat ctatgacagt
     6541 aattacaaga gttggtttta tctcaagtca gatggggctt atgctcatca agaatggcaa
     6601 ttgattggaa ataagtggta ctacttcaag aagtggggtt acatggctaa aagccaatgg
     6661 caaggaagtt atttcttgaa tggtcaagga gctatgatgc aaaatgaatg gctctatgat
     6721 ccagcctatt ctgcttattt ttatctaaaa tccgatggaa cttatgctaa ccaagagtgg
     6781 caaaaagtgg gcggcaaatg gtactatttc aagaagtggg gctatatggc tcggaatgag
     6841 tggcaaggca actactattt gactggaagt ggtgccatgg cgactgacga agtgattatg
     6901 gatggtactc gctatatctt tgcggcctct ggtgagctca aagaaaagaa agatttgaat
     6961 gtcggctggg ttcacagaga tggtaaacgc tatttcttta ataatagaga agaacaagtg
     7021 ggaaccgaac atgctaagaa aatcattgat attagtgagc acaatggtcg tatcaatgat
     7081 tggaaaaagg ttattgatga gaacgaggtg gatggtgtca ttgttcgtct aggttatagc
     7141 ggtaaagaag acaaggaatt ggcgcataac attaaggagt taaaccgtct gggaattcct
     7201 tatggtgtct atctctatac ctatgctgaa aatgagaccg atgctgagaa tgacgctaaa
     7261 cagaccattg aacttataaa gaaatacaat atgaacctgt cttaccctat ctattatgat
     7321 gttgagaact gggaatatgt aaataagagc aagagagctc caagtgatac agatacttgg
     7381 gttaaaatca tcaacaagta catggacacg atgaagcagg cgggttatca aaatgtgtat
     7441 gtctatagct atcgtagttt attacagacg cgtttaaaac acccagatat tttaaaacat
     7501 gtaaactggg tagcggccta tacgaatgct ttagaatggg aaaaccctta ttattcagga
     7561 gaaaaaggtt ggcaatatac ctcttctgaa tacatgaaag gaatccaagg gcgcgtagat
     7621 gtcagcgttt ggtattaagc gatgatttga aagagggatg tgatagtagc accctctttt
     7681 tctttgtttt atgatagttc atcctcgagt aaattcaaga tcttgctcgg aaatgaagct
     7741 tatgaagctt atatagtaga ttgaatatag ataaagacct tgtgattggt aaaacatttt
     7801 agaaattcat ttacctttcc taatcgactt ggtttcatct tatttcaatc tattatagta
     7861 ttggggaatt tcttcaaacc acatcagctt ggtcagttct acctgcgacc tcaaaacttg
     7921 tgctttggtc aagctgggtt tagtttccta gtttgctgat ggatttccat tgactataag
     7981 catccaaccc tctttttgtc ttctaaagaa ttcttaaatt atcagtctat tgcaactttt
     8041 ctcatataag ttctttgtct tgctattggt tttccttagt agtatactaa ggtagtaatc
     8101 attaagaagt ggttacaaaa aataatgaat gaggtaaaat aaaatggtag aattgaaaaa
     8161 agaagcagca aaagatgtcg caacattgac aaaagcagcg ccagtagcat tggcaaaaac
     8221 aaaggaagtc ttgaaccaag ctgttgctga tttgtatgta gctcacgttg ctttgcacca
     8281 agtgcactgg tatatgcatg gtcgtggttt ccttgtatgg catccaaaaa tggatgagta
     8341 catggaagct cttgacggtc aattggatga aatcagcgaa cgcttgatta cactcggtgg
     8401 aagcccattc tctactttga cagagttcct tcaaaacagt gaaatcgaag aagaagctgg
     8461 tgaataccgt aatgttgaag aaagcttgga acgtgttcta gctatttatc gctacctcat
     8521 cactcttttc caaaaagcgt tggatgtgac tgacgaagaa ggcgatgatg ttacaaacga
     8581 tatttttgtt ggtgctaagg ctgaacttga gaaaacagtt tggatgcttg ccgcagaact
     8641 tggacaagca cctggtttgt aagaaacaag acaacataga taaatctgta ggaaatttct
     8701 tacagatttt ttctattttg taagctattc caaaccagtc tttttttgag ttttttgata
     8761 aaatagtact atcagtgaaa aggatggaag catgactaag aaaatcgtag ctatttgggc
     8821 ccaggatgaa gagggtgtga ttggtaagga caatcgtctg ccttggcatt tgccagcaga
     8881 atttcagcac tttaaagaaa caactctgaa tcatgctatc ttgatggggc gggtaacctt
     8941 tgatggaatg gggcgtcgct tgcttccaaa acgggagacc ctgattttga cgcgtaatcc
     9001 ggaagaaaag atagatgggg ttgctacttt tcaggacgtc cagtctgttc ttgactggta
     9061 tcaggctcaa gaaaagaatc tctacattat cggtgggaag caaatttttc aggcttttga
     9121 agcttacctt gatgaagtga ttgtcactca cattcatgct cgggtggaag gagataccta
     9181 tttccctgaa gagcttgact tgtctctttt tgagacagtt tcaagcaaat tttacgccaa
     9241 agatgagaag aatccttatg attttaccat ccaataccgc aagagaaagg aagtttaatg
     9301 gaacggagtt tatttggtct attcacggct tttctgtgtt tcatctgctt tctggcagga
     9361 gcacaagctt ttcgtaaaaa gcgttacggg ctttctatcc tcctttggtt aaatgccttt
     9421 accaatctgg tcaatagtat tcatgctgtt tacatgacct tattttagat agaatgacag
     9481 tatagaatgg aaggaaatca tgtctacaaa tagaaaaaat gatatgatgg tttattgctc
     9541 attttgtggc aaaaaccaag aagaagtaca aaaaataatt gctggcaaca atgcttttat
     9601 ttgtaatgaa tgcgtggagt tagctcagga aatcattcga gaagaattgg ttgaggaagt
     9661 cttggcagac ttgtctgagg tgccaaaacc aattgaactc ctccatatct tgaaccacta
     9721 tgtaattggt caagatcgtg ccaagcgtgc cttggcagtg gcggtttata accactacaa
     9781 acgcatcaat ttccacgata cacgcgaaga gtcagaagat gtggatttgc agaagtcaaa
     9841 cattttgatg attggcccaa ctggttcagg gaaaactttc cttgcccaga ccttggctaa
     9901 gagcttaaat gtaccttttg ctattgcgga tgcgacagct ctgacggagg ctggttatgt
     9961 gggtgaggat gtggaaaata tactcctcaa actcttgcag gttgctgact ttaacatcga
    10021 acgtgcagag cgtggcatta tctatgtgga tgaaattgac aagattgcca agaagagtga
    10081 gaatgtgtct atcacacgtg atgtttctgg tgaaggggtg caacaagccc ttctcaagat
    10141 tatcgaggga actgttgcta gcgtaccgcc tcaaggtgga cgcaaacatc cacaacaaga
    10201 gatgattcaa gtggatacaa aaaatatcct cttcatcgtg ggtggtgctt ttgatggtat
    10261 tgaagaaatt gtcaaacaac gtctgggtga aaaagtcatc ggatttggtc aaaacaataa
    10321 ggcgattgac gaaaacagct catacatgca agaaatcatc gctgaagaca ttcaaaaatt
    10381 tggtattatc cctgagttga ttggacgctt gcctgttttt gcggctcttg agcaattgac
    10441 cgttgatgac ttggttcgca tcttgaaaga gccaagaaat gccttggtga aacaatacca
    10501 aactttgctt tcttatgatg atgttgagtt ggaatttgac gacgaagccc ttcaagagat
    10561 tgctaataaa gcaatcgaac ggaagacagg ggcgcgtgga cttcgctcca ttatcgaaga
    10621 aaccatgcta gatgttatgt ttgaggtgcc gagtcaggaa aatgtgaaat tggttcgcat
    10681 cactaaagaa actgtcgatg gaacggataa accgatccta gaaacagcct agaggtgact
    10741 atggaactta atacacacaa tgctgaaatc ttgctcagtg cagctaataa gtcccactat
    10801 ccgcaggatg aactgccaga gattgcccta gcagggcgtt caaatgttgg taaatccagc
    10861 tttatcaaca ctatgttgaa ccgtaagaat ctcgcccgta catcaggaaa acctggtaaa
    10921 acccagctcc tgaacttttt taacattgat gacaagatgc gctttgtgga tgtgcctggt
    10981 tatggctatg ctcgtgtttc taaaaaggaa cgtgaaaagt gggggtgcat gattgaggag
    11041 tacttaacga ctcgggaaaa tctccgtgcg gttgtcagtc tagttgacct tcgtcatgac
    11101 ccgtcagcag atgatgtgca gatgtacgaa tttctcaagt attatgagat tccagtcatc
    11161 attgtggcga ccaaggcgga caagattcct cgtggtaaat ggaacaagca tgaatcagca
    11221 atcaaaaaga aattaaactt tgacccaagt gacgatttca tcctcttttc atctgtcagc
    11281 aaggcaggga tggatgaggc ttgggatgca atcttagaaa aattgtgagg aaaagaaaat
    11341 ggcaaaaaca attcatacag ataaggcccc aaaggctatc ggaccctatg ttcaaggaaa
    11401 aatcgttggc aaccttttgt ttgctagcgg tcaagttccc ctatcccctg aaactgggga
    11461 aattgtagga gagaatatcc aagaacagac agagcaagtc ttgaaaaaca tcggtgctat
    11521 tttggcagaa gcaggaacag actttgacca tgttgtcaaa acaacttgtt tcttgagcga
    11581 tatgaacgac tttgttcctt ttaatgaggt ttaccaaacg gccttcaaag aggaattccc
    11641 agctcgttca gctgtggagg tagctcgtct tcctcgtgat gtaaaagtcg aaattgaagt
    11701 catcgcagag attggataag ctagttgaag tttggtgttg ccaaacttct tttgatataa
    11761 ggagaaaaag atgacaaaga aacaacttca cttggtgatt gtgacaggga tgagtggcgc
    11821 agggaaaact gtagccattc agtccttcga ggatctaggt tatttcacca ttgataatat
    11881 gccgccagct ctcttgccta agtttttgca gctggttgaa attaaggaag acaatcctaa
    11941 gttggccttg gtagtggata tgcgtagccg ttctttcttt tcagagattc aagctgtttt
    12001 ggatgagttg gaaaatcaag atggtttgga tttcaaaatc ctctttttgg atgcggctga
    12061 taaggaattg gtcgctcgtt acaaggaaac cagacggagt cacccactag cagcagacgg
    12121 tcgtatttta gatggaatca agttggaacg tgaactcttg gcacctttga aaaatatgag
    12181 ccaaaatgtg gtggatacga ctgaactcac tccacgtgag ctgcgcaaaa cccttgcaga
    12241 gcagttttca gaccaagaac aagctcagtc tttccgtatc gaagtcatgt ctttcggatt
    12301 taagtatgga atcccgattg atgcggactt ggtctttgat gtccgtttct tgccaaatcc
    12361 ctattattta ccagaactga gaaaccaaac gggtgtggat gaacctgttt atgattatgt
    12421 catgaaccat cctgagtcag aagactttta tcaacattta ttggccttga ttgagccgat
    12481 tctgccaagt taccaaaagg aaggtaagtc cgttttgacc attgccatgg gatgtacggg
    12541 tggacaacac cgtagtgtgg catttgctaa atgcttggcg caggacttat ccaagaattg
    12601 gtctgttaat gaagggcatc gcgacaaaga ccgcagaaag gaaacggtaa accgttcatg
    12661 agaaaaccaa agataacggt gattggtgga gggactggaa ttcccgtcat tctaaaaagt
    12721 ctgcgggaaa aagatgtgga aatcgcagct atcgtgacgg tggcagatga tggtggttct
    12781 tcaggtgaac tccgaaaaaa tatgcaacag ttgacaccgc caggtgatct tcgtaatgtc
    12841 cttgtggcca tgtcggatat gcctaagttt tatgagaagg tctttcagta tcggttctct
    12901 gaggatgccg gagcctttgc tggccatcca ttgggaaatc tcatcattgc tggcttgtca
    12961 gaaatgcagg gttcaaccta taatgccatg cagttattga gcaaattttt ccatacaaca
    13021 gggaaaattt atccttccag tgaccatcct ttgacccttc atgcagtctt tcaggatggg
    13081 acagaagtgg ttggagagag tcatattgta gaccatcgag gcatagttga caatgtctat
    13141 gtgactaatg ctctaaacga tgatacgcct ctggccagcc gtcgagtagt gcagaccatc
    13201 cttgaaagtg acatgattgt cctagggcca ggttccctct ttacctctat tttgcccaat
    13261 atcgtgatta aggaaattgg gcgggctctt ttggaaacca aggcagaaat tgcctatgtc
    13321 tgcaatatca tgacccaacg tggggagacg gaacacttta cagatagcga ccacgtggaa
    13381 gtcttgcatc gtcaccttgg tcgccctttt atcgacactg tcttggtgaa tattgaaaaa
    13441 gtgcctcagg aatacatgaa ttccaaccgt tttgatgaat acttagtgca agtggaacac
    13501 gattttgtag gtctttgtaa gcaagtttcg cgcgtgattt catctaactt ccttcgtctg
    13561 gaaaatggcg gtgccttcca cgatggagat ttgattgtgg acgagttgat gcgcattata
    13621 caggtgaaaa aatgagtttc acagtagcag taaaagaaga aatcctgggc caacaccatc
    13681 tgagctggca tgaattatct gccattatca aaatgtctgg tagcatcggt ctctcgactt
    13741 cgggcttgac tttgtcagtt gtgacagaaa atgccaaact ggcccgtcac ctctatgagt
    13801 cctttcttca tttctatgaa atcaaatctg aaattcgtca ccaccaacgt agcaatcttc
    13861 gcaagaatcg cgtctatacc gtttttacag atgaaaaggt gcaggatttg ttaagtgatt
    13921 tgcacttggc agactctttc tttggcctgg aaacaggtat tgatgaggcg attttatcgg
    13981 atgaggaagc aggtcgtgcc tatctctgtg gcgctttctt ggcaaatggg agcattcgtg
    14041 accctgagtc aggcaagtac cagttggaaa tcagttctgt ttatctggac cacgcgcaag
    14101 gaattgcctc ccttctccaa cagtttttac tggatgccaa ggtgcttgag cgcaagaagg
    14161 gggctgtgac ctatctccag cgagcagaag acattatgga cttcttgata gtaatcggag
    14221 ccatgcaggc acgtgatgat tttgagcggg ttaagatttt gcgagaaacc cgtaacgacc
    14281 tcaatcgggc taataatgcc gagacagcta atatcgctcg gacagtttct gccagcatga
    14341 agaccattaa caatatcagt aaaatcaaag atatcatggg cttagaaaat ttgccagtag
    14401 atttgcagga agtggcgcaa ctgaggattc agcacccaga ctactctatc cagcagttag
    14461 cagatagtct cagcacccct ctgaccaaaa gtggtgtcaa tcacagactc agaaaaatca
    14521 ataagatagc ggatgaatta taacgatata aagcaacccc ttgaattatc aatatcttca
    14581 aggggttttg ttgtgttatg aaatcaaaaa ggggcaatca aggggcagaa ttaaaaaata
    14641 gtatctaatt tattgaccag tttatcctcc atatcttcag tagtatgaga atagatttcc
    14701 aaagtcattt ttgcatttga gtgcccaact cgatccatta ttgattttat tgggaggcca
    14761 gactctgcta aaaacgaaat atgagaatgc ctaaaaatat ggctagataa gttttttcta
    14821 ttttggcctg ttttccatat ttttttaata tctgtatgaa gcaagctatt gttgtaggtt
    14881 gattccattt ttcaaaacag aaaatataat catcgcttga caatggctgg aaacgttcgc
    14941 taagtcgtac tatttgtctt tgaatagctt ctatgacact ctctgatact ttgattgtcc
    15001 gtattgaatt tgtagtcttt ggtagcgtct tgattttgtt tactgaatca aaattacctg
    15061 tgatctcaat tttgttgttt tcgaagtcta tattcttcag ttgtaaggca gttaactcac
    15121 catatctcat accagttaat gtcagcacaa gaaccatatc agcgtacttt tggtgatatt
    15181 ctcgacgatt aaggacatcg acaagtgctt ttatttcttg catggtgaga aagttgttac
    15241 gctttttttc cagttcttct aaagtctttg gtttttgagg aatcgtagta caatcgacct
    15301 cgttgttttc aatgtaagag tattgaacag cgtaattaaa gatacctctg agcctatgcc
    15361 gtactttttt agctgtaata tatccgttgc tttcaataat tttttcaata gcctcttgaa
    15421 gaaaacgcct gtcaagatta gcaagtatgg tatcggatgg tatgacttcc ttcatcttct
    15481 tatcaactga tttacaatta tgttttgttg attcctttac tgtttgcgcc catgatttat
    15541 aaaaaaggtt atagatttct tcaaatgtaa tgctttctac ttgttttgtg ctaagttttt
    15601 tatttatctt ctcttgcaac aagatagcag cttgatttct tgcctgggga gttttcttct
    15661 ccatggttac tgaaactttt tttaatttct cagtatatgg atctttgtat cgctcaaaaa
    15721 atttatattt gccgtttggt aattcttcca tccacattga ttttaacctc actttttgat
    15781 aaaatgggta tagtaaaaag ggctttttaa tgccttttac tatactggat atcctcacac
    15841 tcaaagtttg gcgatggcga gtgtgaggat tttttgtttt tatagttatt ctgatgaact
    15901 atcctatcaa ttcataatat tcgtcaatta ccatcaattc gtctgtgact gttctaagct
    15961 catgtttttg catgaactgg atatagttga aagattgatg gtcgtctgat agtgcaagtt
    16021 cttcttctag caacttatga atcatgtgcc tattagcctc attctcgcac ctagtgtggt
    16081 tatttttata tagtgcagta gaatgttcca gatgtcctaa ttcgtggtat atgacccgtt
    16141 tttttgcgtt ttcggacagt tcacggttta taaagataat actaatctct ttgatgtaaa
    16201 ctccaggtct ttgccaaagt tcattatcaa agtaagcgag agtgacaccg tgtgagtcta
    16261 ctagctcttc aatagtcata ggctatcatc ctttttgtgt tattttgttg ttaaaatggt
    16321 tacgatgatt gctagtatcc ccagtaaagt actaactaac agtccgataa accaatacat
    16381 gaactctttt ttgcttttag cttgctcttc taatctctta ttctcttgag ttagaaacat
    16441 agtctctaca cgtttctcga aattatcaaa ttttaaatca acttgttcaa attttaagtc
    16501 gattttttca aatccgctac gcatttcttg tctgagttga tcaactttta aatcaatttt
    16561 ctcgaatcca tgctgagtgt cagaattaat tttatcaagt tttaagtcga tttcagattt
    16621 gctgtaagta tcttgtgaca taatattttc ctccgataac atttctgact ccattatatc
    16681 atgattttgt atgggtacag ttttcgatga agatttagaa gcgtaaagtg aagaaacttt
    16741 ggaaggggta ggtttgaaat tatcttgtgt gtccggcatt gtttaccccc actttccaaa
    16801 atgatgataa ctatatgctg tatctacttc ctgaccgttt tcgtccatca agacgaagaa
    16861 aaagtaaaaa tcgctcggct tttctatcgt aaaattaaag ctaaaatttc cagtggccat
    16921 accaaagccg tcttcaagta gcacaaattc ttgtctcgca atatttattc tcgtagcatg
    16981 gacagggtaa gacgtgccat tagggaagtg ggcagttaga gacaagatat aatctgtttc
    17041 aggtcttaga ttaaagaagt ctagaaatgc aaccaaagat gtcgaaccag gaaacatatc
    17101 gaaattggtt attgttccta gcaattgagc tgtttcagga ttgacaatac tgattgctgt
    17161 catcttttct ctgaacggat tcttcttcat tggaatgttc gtcatactat ctacctctca
    17221 aataaatctc aataatgttc tgaatggcat cgatgtcttc ttctgtaagt ggcttgccat
    17281 cgaaggtctt ggcattctct gccatctttc ggaggtcgtc agacgtgtat cctgcgattg
    17341 tatcatcacc agcaatagca ggattatctg tgcgaccaag taagtagtct gtagatacat
    17401 tgaagtagtc agcgattttg gatatatgtt cagcagatgg agtcttttta tttttaagac
    17461 tgtaaatgta atttttcccc aaaccaaccc tatcttctaa agtgtttaga gaaattccct
    17521 gttttttagc taaactttta attttttcga atgtctcaaa cattgataca tcaacctttc
    17581 tgaagcatta caaaaaaata ttttattttt tcgcctaaaa cacttgacaa aaattagacg
    17641 aacaactaaa atagtatttg taagtaatta acaactaaaa aaacaactaa gaaataaatt
    17701 ataaaaatgt ttttgcgaac ggtatttata gatttattag tgtttttatt atgctttcat
    17761 tttacacgaa catctaaaaa aagtcaagcg aaaagataaa aaatagttac atttttagtt
    17821 gtttcttatt tacaaaataa gtaaaaagga ggaacgtgtc gatggtaagt attcttaaaa
    17881 atttagaaca agaaaaagac caccttgaaa aaatcattaa ggtagtcagc gctggtggta
    17941 aatttctgag attgccatat caaaaaaagt cacgctcgat tagtgagaat ctgaaattga
    18001 tttctcaaaa tcttgataaa ttgagcgagc aagttcaaca aaccacgaat cagcattcat
    18061 gatttcaaga tgacgaaaga aacctgtttt ggtttccagt tcagaatcgt gtgcataacg
    18121 taatatttct ctagcaaaga tggtttcaaa atcaaaatct ttaccatcgt cgtagatgtc
    18181 gcgtttgctt gctttgagta aatattcttt aaaagtcata aggttaactc ctttctgctt
    18241 atattatagc agaaaaagaa gaaagaaaaa gaaaggagaa aatatgccag atatcgcaaa
    18301 cggtcgcgaa agagttattg ctttcttgaa agagaaaggc attaaaaaag caactctagc
    18361 ggttgcttac ggctttaaac gacaggaagt gacaaacatt ctaagtggaa cgactaaagg
    18421 tccacgagcg aacagtttca ttcttcaggt tattgaagat tatgggattg agtaggaaag
    18481 atttgaggag taggaaaatg agaccaagac gatatccgta tagtgggaaa aaagagtcca
    18541 cctttgtgaa ggcagaccct gaattggtag aaaagattct aagaaacatc agttttcttg
    18601 agcatttaca agttgtgtta gcaacgaaac tgtagtttct gtgctgagtt tatgagcgac
    18661 aagaacgcta tagaaaatag cttctctaat ctctatgtcg attggatatt tttcaaatac
    18721 ttcatccaac atatctttga taaccggaat agcatcgttg ataatgtctt gagaaacttg
    18781 taaaacatct tctttagtta atttagacat atagttttcc tcctttctga tggaattttg
    18841 actaaaacgg tgagaggtcc tagtcaaaat tattatagca atttaggagg aaattacatc
    18901 agtcttgagg ctgatatagg aggttgaatg gaagataaaa tcattgaact tgctgattac
    18961 ttcatcagcg aatctacaac gtacagagaa gcaaaaatag cgtgtgagaa gctattgaaa
    19021 caagtcagcc atgagataga actcagggcg atggaaagta ggacagtcta gaagacaaca
    19081 aaaagcacct gacggcaatc aggcgcatga caaaattatt caagaaaatt ataacacgaa
    19141 aggagcaaaa atggaagcag ttgaaattgt aagaattaaa gatgtgatta ttgaaaaagt
    19201 ctctgctaat gatgaagagt taaaacgtat ctttggatgt tcaaaacgac aagcaggaga
    19261 gcgaagaaga gaaatgcaaa aactccctag tcagcaaaaa catcttttgg atagtggaca
    19321 acttgtaacg attaaaggtt tctatgaata cttgcaatat cgtggaacta aagcttggaa
    19381 aaaagaaatg gaaacaagca agaaaatgag gtcagcagga tgaacctact atcaagaatc
    19441 aaaaactatt tttcggaaga ggtcaaagaa actaatctcg actggaaaga ggtcgcttta
    19501 gacctcaatc aatcactaat tgaaacacag gaaaaacttc aagaagcgaa tcaagaaatc
    19561 gcagacttga agaaaatcgt agcaatctac aaagaaaagg agaaagaaaa atgatggaat
    19621 acatttacct ggtaataatc gtaggaattg gactatggtc gctagtaaat aaactagatg
    19681 accacgctga aatgaaacaa aaagagcgcc agctgatggc aaacaatgtc gcacggatga
    19741 atctgagaaa ttcagataag caatttactt atgatgtaga accgcctgaa gggttgaaat
    19801 aaggaggaga aacatgactc aagcggaaca aattagggaa tattatagag agcaccctgc
    19861 tgcctcatgt gatgaagtgg ctgaggttgt cggtacaaca aaaagcaacg taagtgcaaa
    19921 cctggccaaa gacatcaagg caggcagatg cgttcgcttg gaagataagt catacgacta
    19981 ctcgccttac tataaccata cacaggcact cactgagttg gttgattgga agaatgatac
    20041 tagacgtgag tgggtggata tgctgacaag agcagcagaa aaagaaacgg ttagcaatgt
    20101 tatgcgtttg ttaatcaaag aagcaaataa attgatgaaa gaggtgacga agtagatggt
    20161 tcgaaataaa ttgacagatt taaccaatac tcttttcgcc cagttggaaa cattggacga
    20221 tagggatctt actgcagatg aattaaagac ggaactccaa cgttcaaaac agatggtcgc
    20281 aatctcaggt caaatcttac aagcaggtca attggcgcta gatgctgaaa aattcaaaga
    20341 caaggtaggt gaagtcaatg ccccgatcgc tttgctggaa ggatgagtat acagagtaca
    20401 tgcatgagat atgccctggc cgattaactc ctgaagtaac caggttacta aatgagaaat
    20461 ttggtacgac ctataccaag actcaaatag gagaagtacg cagacgttta gggttacctg
    20521 ttggaaaagt ctatcaaggt aaattgttga caaaagaaca acatgattac cttgtgtcaa
    20581 tccaaaaaaa taagatttct cgcgatgtcg caaatgaaat gaacctaaaa tttggattat
    20641 cactgactga gaaacagatt aagagttatc gaagaaataa taatctacat agtggtttga
    20701 caggaagatt cgagaaaggt cagactcctc acaataaggg gaagaagtac cccaatatgc
    20761 caaaaaacag cgggcagttc aaaaaaggta atcgacctct gaattatgta cctgtcggta
    20821 ctatcaacta cacaacagac ggttatccaa aagaaaagat tggagaacct aatcaatggg
    20881 ttttgaaaca ccgcaaggtt tgggaggaac atcacgggcc aataccaaaa gggcattcga
    20941 ttgtcttctt ggacggtgat aaaacaaact atgatatttc aaacctggca tgtttatcta
    21001 aaaacgaaat tgctagaatg aatcaaaatc atttatttac gtccaacgct gatttaacca
    21061 aatcaggtat tggactaaca aaactcacaa acaaaatcag agaggtagaa aaaaatggct
    21121 agtttatacg aactgacagg tcagtttctg acagtttatc aaatggatat tgatgacgaa
    21181 acaaaaacgg acacacttga ggctatcgat tggcaagaac aattcgaaca gaaagcagaa
    21241 ggatatgccc atgttatcaa gaatctagaa gccgacgtgg ccatgtacaa ggctgaggaa
    21301 gagagcttca aagccaagaa acaggtggca cagaaaaagc tggattatgt aaaggataac
    21361 attatggcag ctatgaatgt cacggggcaa accgaagtta agagtggtgc cctgattata
    21421 aaaattgcta agaatccaga atcagtcaag gtcaacgaag acgaccttcc gaaaaaatat
    21481 tttacaaaaa aagtgacgct tgcgccggac aaaaaaacac tcaaagagtt gcttaaatct
    21541 ggcaagaaag tcaaaggtgc ggaacttgtc cggacagaaa agttggtgat taagtaatgg
    21601 aattgatgaa taaaacacga gtaacagatt cactagcagt tgtgattgga ccagaatcga
    21661 ttgaagtact tgttactgaa ggttttctat ttgatgttgc gattcgtttt gtaaaagtag
    21721 acgaaacaaa tcttgatcaa ggaaatgaaa agccagtatt cactccggaa tacaagctgg
    21781 tcacagttgc taaatacaag gaaaaaccta tctttgaatc ggaggaagat attcgaaaat
    21841 ttgagaagca agcaaaagaa gttaaatcgc tatttgcctt tgcaaaggta aataaacaaa
    21901 attggtttaa cactgccctt tatccaggag tgctgactga gaaagttggt gtttgatgaa
    21961 aattttagct attgatccaa gcagtaataa aattgaaacc agcacaacag gagttgtctt
    22021 gttggataat gcaagattag ttgatagctg ggttgtctct tatggtatga gaggtttcgc
    22081 tgattggttt cacgaaatcg gaacaaatct tgaattcgat gtagttattg ttgaagaatt
    22141 taaggcgagg gataacgaca agtcgaaaga taatagcgtg gcagaaacca tcgcctatat
    22201 ccaactttgc tatccaggtg ccattcttca attcaatgca ggttacaagt cggatattcc
    22261 aaacgatctt ttgaaaatct tagacctttg gaaatttgaa aaaagtcatc atcaagatat
    22321 tcgagcagca gcaagacttg gattattttg ggcaatgaga aatgatattg aagaagtggt
    22381 tcatgatatc ggaaaggtgg tgagtgagta tcacaataac gctaagaaag tggcaagctg
    22441 aagcgattaa aagaagtgaa catttatcta atggaatctt tttagaggct cttgggggca
    22501 gaggcaaaac tatctgtgca cttgctattg caaaacataa aaaagctaaa aaaatcatca
    22561 tcacaaacaa tcgactagct attctgaatg gttggataga tgcagtcaag tttatgaatt
    22621 ttgataaagg tgttgagatt atcattcaga cagatagata tcttcaaaat caagtcaaaa
    22681 aggggcataa attagattgt gatgtgctga tagtagacga atggcagaat atgtcttctg
    22741 acaaacaagt ggccttatat cgcaaaataa agcgaaaata cacgataggt ctttcagcga
    22801 caccaattcg gaaaaaagga caaaatttct atccacttga aaaaacggta tttggttggg
    22861 caaccccaaa taataaattt gactggcaaa agactcatgg aaaaatggtc tatgatccat
    22921 ttagctattc aaaagagaag tgggaagatt ttcaaaatta tgaaagttat atctcgagct
    22981 tgcctaattt cttccgctgg gaagagattg aaggaattga gaatgcagtt gagaataacg
    23041 gttttgagat taagttttac caaaagagag tcgcctctgg caatccagaa aaacttgcag
    23101 aatttagaaa actaaatctt gtaacagtgg acggcaaaac tgcaatggcc aagcaatcgt
    23161 ttggaagaaa gacctttgaa cgctacctta atcaaacagg cgtagcagtc gattttccaa
    23221 aattaaagcc agtaaatgcg gatacgccat tgatgttaca acttgacggt ttaatcgaac
    23281 gagcaccaca cgatatgttg attgtcagta aatctaagca gattgtcaac gtcattagcg
    23341 agcgccatcc tgaaattgga atctggacgg gcgatattca agaaggactt tataagaaat
    23401 tcgtggttgc tactagtcaa gtgttaggtg tcggagtaga cggcttgcaa cacaaatacc
    23461 aaactattgt cgtattggat ccagtagaag aaggttctgg agaatatgat gattatcgac
    23521 aattgctctg gcgtataaca ggaagtcgtc agcagcatga tgtaaatgta attgaatttt
    23581 attataaaga aagttaaaaa aaagaggaaa acaaaatgaa taaaacaact gaaatgatcg
    23641 tatttcgtag ccgtaaaact ggagaatttc ttaattctta caaggacaga agttctttag
    23701 catttgcagc tgacttttgc agcttggaat attgtttgaa gcttcctcgt aaaaaatacg
    23761 aagacaacaa aaagacttac aaggctcttg ctgcagcttt tgactgtgaa attgtcgcag
    23821 ttgaagcgga atacaaattg acctatccga atggatcaga agttgaacct atcaagcgtg
    23881 accgttcatc aattgaggac atgattaagg atattattgg aggggttctc taatggcatt
    23941 tacacttcca gcaaataaac cacaagttcc taaagatacc ccacgaaatt ttttcatcta
    24001 cggtgaaacc atgagcggaa agtcttatct tgcaaatgaa ttcccaaatc caatcgtttt
    24061 gaacacagac gggaatgcag aagctaacac tgttccaagc attcagctga tcaatgaaaa
    24121 agatgacaag ggacgaatta ccaattcagt aattaagcag cttggagata tcttgcttgc
    24181 tctccagaca cagaagcact cttatgaaac agtcgttatt gatgtaattg acgatgttat
    24241 tgaaatgatt aagattgcag tttgtgatga attaacccca gttggtaaac ctcgcttgaa
    24301 atccttgtcg gaaattccat acggcaaagg atacgacttc tttaaccaag ctatcacaga
    24361 attagtcatt gacctcaaag cattgccaat gaatgttatt tacatcagcc gtcaggtatc
    24421 tgaatatgat gacaatggca atgccaccaa agacaagcca agcttgaaag ataagtatgt
    24481 caatcttatc aatggaaact ctgatttgat gatccacact gaaaaactcg gcaacaacta
    24541 caaccgtgag gttgaccgca agcgtaagac ctattatgcg gaccaggttg atgacaaggc
    24601 catcttgaaa atcttggcaa ctatccgtgg ggctgttgag cctgcaaagg gcaagctagc
    24661 ccctaaaaaa gaagcagcta agacaactaa accagctaag accgaaaaaa caaaagaggc
    24721 acctaagaaa gaagttgact ctgatgatga actattttaa gaaataaagg agaatacaca
    24781 tgagcttact agatatcgca aaatcaatca aaaaagaggg ctttgaccca cgcaaagaca
    24841 gcgccaacgg tcctgcacca atcccagctg gtacttatcc agtagtcctg aagaaagcaa
    24901 ccttcaacgt atcggacaaa ggctgggaaa gtcttggtta tcaatttgaa atccgtggcg
    24961 gtgattacag tggacgctct gaatttgcaa catttggcac actgactgaa tggaacggta
    25021 agaaccttga ctgggcagtt gaacgcacta tgaaattctt tatcaaagcc ttggttcttg
    25081 ctggcgacag tatgcaagga aatgaagaag acggtaaagc cttggaagag gctctaaaac
    25141 gtaaggcagt tggctcttac tacaaccttg ttatctctgt gactaagggg aaagatggcc
    25201 gtgagttccg aaactatgac cttgaagaag aagaagcaca accgctgact gaagctgata
    25261 ttgatgacga tgacctccct ttttaagaaa taacaagttc tgggtcattg atgaaactga
    25321 tgaggaattt ggtcctttca cgacagtaga agaggcttat acagctatgc taacatactt
    25381 ggatatgact gaagccgaat atcagtcaaa ctatacggcc caggaacttg tttatattta
    25441 caaagaggag aaaaaaccat gccgtcgatg aaagaatacg cattacagta ccaaaagtta
    25501 gggttctcag tcattccaat caatcctaaa aacaagatgc ctttgattga ttttgctgat
    25561 aagccagcca tgactccatc tgagattgaa aacttttggg acggctaccc taatgcaaac
    25621 attgccctaa agactaccaa cttctttgtc attgatattg acaaacacgg caaatcgaac
    25681 ggttttgaat cgctaaaaaa atggaaacat ctaaatttaa tcgaaccgac actgcaagct
    25741 aagacggcta gtggcggtaa acatctattc tacttcaaac gagaagatga gccgatcact
    25801 cagatgattg gattcttgcc tggtgttgat attaaggctc acgaaaataa ttatgtgtta
    25861 gtcgcaccct ctgccacaga taaagggcag tatgagtggg atctggaaaa gtctaaggaa
    25921 ggtggcacga tggtcactcc ttcaaaagat ttaatccagt ctataaaaaa acagtatggc
    25981 gaaactcacg gttataagta tgatggtaag gacggtctta gggatttagt tagacgttca
    26041 catactagag accgaacaca gactacagat ctctttgaaa ccatcgccct tggttttggt
    26101 gatgaaggtg gacgaaatga caaactagca aaattcgtag gtggtctctt atatcgtgcg
    26161 gtcgacgatg gtgtagttgt tcaacttgca agattagcaa atgcaaatag tccaaaccct
    26221 ttgcctgaaa aggaaatgat gcgtactatt gaaagtatga ttaaaaaaga taggaggtga
    26281 ttgtgattgg taatgtagta agtattgact cacaacctaa gatgataacg actgccaagg
    26341 gagacatcaa ggccaacagt ccaagtaatg tgttgatgtc tttcaaagct gatgatcagt
    26401 tgagtattta cctaaagcac aacgattttt cccaagagca tgaactcctt aaagatatca
    26461 agatcggcaa cactcttttt aaaaaaggtg agctcccttc taactttgat tcagtcgtaa
    26521 aagtttactt tgaaagtgtg ttaggtgttg ctttctctag ccaggcgatg ctggacggca
    26581 tggaaacttt tttctcagaa cgttcttaca atccagtcat tgagtatatg gaaaaggctg
    26641 ctggaaaatg ggatggtcgt aaacgaattg accgaatgct tcaggtttac ctgggggctg
    26701 aagatatccc tttagtttct aaaatcgctc aaatgtggct agttggtgca gttgctaaag
    26761 tttatgatcc atacgttaag tttgactatg ttctggatct agtcggtgga caaggagttg
    26821 ggaaaacatc cctccttcaa aaattgggtg gcgaatggta tacggatgcc gtaacagatt
    26881 tctctaataa agataattac gacattatgt taaagagtct aatcgtcaac gacgacgaaa
    26941 tggtggccag taatcggatg agctttgcag aaactaaggc ctttatttct aaaactagcc
    27001 tacgttatcg taaaccatac atgaaacgca cagaagagtt tgccaaaaac ttcattttag
    27061 ctagaactac taaccaaaca gaatacctca aggacaaaac cggtgaacgt cgttttctac
    27121 cagttatggc agatagtaaa cggcaaaaaa aacatccaat ggaaatcgag ccagagacaa
    27181 ttgaacaaat ctggggcgaa gccgttacaa tctatcgtgc tggtgctgat ttgatgtttg
    27241 atgaaaatac agaggatgaa ctgaatatct accgtgaaca gttcatgtat cgtgatgaag
    27301 ttgaattaca agtgcttgaa tatcttgata tgcccgtccc tgaaaattgg caaaactggt
    27361 ctattcagca acaacatcaa tacacaagta aatatttcga taatagtagc gactttgatc
    27421 ctggaagcaa aaaactagat aaggtctcaa ctcgtgaaat gatgtacaac ttatttatga
    27481 gaaattcgaa tgacaggaag ctgtcaacga agattaacat gatcatggat aatcatcctg
    27541 attggaaaaa aagtgttttc cgggcaggag gtaaaagtac aaaagggttc gtaagagtga
    27601 aagattcgga aaaaactaat cggtagcaat taaaaaatta tcggtagtca tcggtagcag
    27661 ttgagggggt agatcggtag cattctaccg ataaaatgag acatcggtag cacatcggta
    27721 gcagtctaac cccttgatat tactgacttt tatttaatat ttatatataa tgctactctt
    27781 ctacctatat ttttaaaaaa agtatataaa ataatagtaa taatagagaa agcctataaa
    27841 atagggattc tttaaaaata ttttttactt tttagaattt atcggtagta cggtagcagt
    27901 ttgaaaaaaa gaggtaaaaa tgtcatacac agtaacacta tattttgaca acatggtaga
    27961 cgaaactcac ctttttaaga aaaagggtga tgctgccaaa tgcaaggcgc agttagaaag
    28021 caagtatcga ggtgatcgaa tgtataaagt aaagatggag gagatggagt aatgagttat
    28081 gatttggaaa tcttagcgaa aatagagagt ggagattata tttgtattgc tgaacctaga
    28141 tatagttctc cgacctacaa tcttggaaaa atgtttaggg tggctatgga ttgggatttc
    28201 gaccaaggca caatttacaa tgttgctgat atttttgaaa atattaaacg tggcataact
    28261 gaattggaaa ggcaacctga aaaatatgta caatatgaac ctgcaaataa atggggaacg
    28321 atcaatgatg cgttatatgt tttgagatcg ttaagggact gtattttaga acaagatatt
    28381 gatacgaaat atttatatgt gaggtggtaa attgaaacga ccaaacagat acccgtacac
    28441 taaaaatcaa tgggttgaag aaaccgttga tcactatact tataaaaacg atatttgcta
    28501 tacaagtcac attttagaaa atagacttac tggagaaatt aaggacaagg agttgaagtg
    28561 atggaagagt taaagcaaaa agttaatgca gtatacaact ggacggtaga agacgggaag
    28621 ccgcaacctc ccaagcaaga tttaccacaa gcagtaaaag accgggtgga ctatttttgg
    28681 gaaatggcag aagatggtat gacgtttatg ggagcgatgg aatgcatctt cgctgatgaa
    28741 aagcctacag actatgattt gggagctact aaggattggt tgccaaaatc taaggagttt
    28801 gatgattggg ttggctattc gccaggaatg tctcaggtag ttattgcagt ttatttgatt
    28861 tatagaggaa actaagatga ataagcagga attgattaaa aaattagagg aacgaagaac
    28921 aataactggg aattttcaag gttatgtagt ttggtggaag gatgtaaaag aaatctttga
    28981 acaactaggc gaaccgcaac cagtcaaagt accgcagtgt gtggcggaat atatagaatt
    29041 taaaaagaaa aacaattttc atgtttacgg tgcaatgaga gtaattgaag atcattatga
    29101 taagaaagtt cctgattggt tttacgaaaa taacatcgaa aaattctgtc ttgcttggct
    29161 taacggctac gaggtcgaaa aagagaagcg gtattttgtt aagattaaag ggaatattaa
    29221 agaaaatatg ttggtttatg gagaactttt gaaaaggtat ttctttacaa aaagctttag
    29281 tttagacgat gttatatatt cccacacccg taaagaacta gaagacgcaa acttcggctg
    29341 ggtgtttgat tgtccaggga ttgagattga ggaggtggag tgatggaaga tgaggaaatc
    29401 ataggcaaca tctacgaaaa tccggagctt ttggaggtaa ctcatgaata aacgtcaacg
    29461 caaaaagaaa attttgaacg gtctgaacaa agaagaaaga taccacagga cgcattgtcc
    29521 tgtctgcgat agcgaagctg gtttattcga cagatatttt aatacgtacg gtttctgctc
    29581 tgaatattgt ggttatgaat actatggaat ttcaagatta taaaataaag gattgaggtt
    29641 aaaatgacat tatttgatga agtgcagcaa ttaagctcag aaagccacgc aaaatggttc
    29701 gagcgatatt ttgagaaata taacctagaa caaaaactaa aaacttctgc tcaaaaaggt
    29761 tatacaggtt atttaatcaa tgtttggtca gttagagacg aatatctcag gaatcgatta
    29821 ggagatgaaa taacgtttga agcgttaaga gaattattag gagctggctt tactgtcaaa
    29881 tataagcttt atctatctaa aaatattttc actggacaag atttcgtttc taacaagaaa
    29941 attcacatta cttggtaaaa caaaaaagcc aagacactct ctgtctcagc aataatctca
    30001 ataatattat tatatcacaa aggagacaga gagtgaacaa ggctaaagaa ctattgaaag
    30061 agttgcagaa tctggacatg gacattcaaa gccgtataga tgaaattaac gagcttgagg
    30121 caggtttgct ctcaagtcct aagtggtcag atgtcaaagt tcaaggtggt caagctagaa
    30181 aagttgatga tgtctatact cagcttgtcg tgatgaaaga ggctatagaa caggatacta
    30241 aagaggttat caacagaaag ctccaactag gtaggatgat caataggctt aaaaatccaa
    30301 aacatagaac tattttgaga atgacttaca tcaataagat gtacgttgat gacatctgtg
    30361 acagcatgag gggcataagt tctcctactt actatcgttt gaagaaacag gcagtaaagg
    30421 agcttgatat tattctttca gaattgatag taaatgatag taactgtaca ggcatgaagt
    30481 ctaaaatctg ttaaaatggt agtatcaaga attgaaaaga gaggtctcag aattggtaga
    30541 tggttacctg taatgtcagg gggctgtaat ggccttggag gttcaaatcc tcccctctcc
    30601 tttgagtgtt tgtgtcctag aatgagttaa atcttctggg tggggattca catatcactc
    30661 attaactcct atcactcatt aacttaaaaa tggttgcgga agcgactgga cctcgcatga
    30721 ttgcgtagct aattatattc cggataagtt ataagctaga gggtttgatt ccctcagagg
    30781 ttttaaagac tacaaaaaat aaaaaagaag tcaaaattta atacgcacgc aaggtagtag
    30841 tcgccttgca agaaggtcgc acatcgtgtg gcttttttga ttgtttgaaa ggtggtgatg
    30901 gaaaattgag tggattgaga ataaaacaaa agagatttgc agatgagtac atcatctcag
    30961 gtaatgcgac ggaagcctat aagaaagcag gttatcgtgt ttctagtgat agagtggcag
    31021 gcgttgaagg acataagtta ctaaagaatc ctaagattaa aagttatata gatgaacggt
    31081 tgaaacagct tgattctgaa aaaatcgcag accagcaaga agtccttagt tatctaacct
    31141 cggtaatgcg aggagagacg caagaacaga ctttgataag catcggagaa ttgggtcaaa
    31201 cgattacgga tattgatgtt ggagcaaaag acagaatcaa ggcagccgaa ctattaggaa
    31261 aacgtcatag gctttggaca gacaaagtag aggcagacgt ttctggaacg gtggtgtttg
    31321 caaatgagtc agacatacca gattaaacaa agtgatattg taatcgacct acctaagaca
    31381 gtaggagctg ggtacggaca gttctggcgc tcaagaaatc tttatcgtgt tgtaaaaggt
    31441 tcccgtggtt cgaagaagtc caagacaacc gctttgaatt atgttatccg tcttttgaag
    31501 tatccctggg gcaacttgct tgttattcgt agatactcga ataccaacaa gcaatcaact
    31561 tatacggatt ttaaatgggc gtgtaatgtg ttgggtgtga ctcatttgtt taaatttaac
    31621 gagtctttgc ctgaaataac tgtaaaagcg actggtcaaa aaatactgtt ccgtggtttg
    31681 gatgatgaac tcaaaattac atctattaca gtcgatatcg gtagtctgtg ttgggcatgg
    31741 ttcgaggaag catatcaaat tgagactgaa gacaagttca gcacggttgt tgagtctatc
    31801 cgtggtagct tagatgtacc tgatttcttt aaacaaatca cagtcacatt taacccgtgg
    31861 aatgagaggc attggctcaa gcgtgtattc ttcgatgaag agacgagacg ggctgacaca
    31921 ttcgctacta caaccactta taaatgcaat gagtggcttg atgaagtcga tatcaaacgc
    31981 tatgaggatt tgtatcatac gaaccccaga cgtgctagaa tcgtttgtga tggcgaatgg
    32041 ggagttgctg aggggttaat ctatgaaaac gtgactgtca aggatttcaa taaagatgaa
    32101 ttactacaag attcagctaa taaattatgt atcggtcttg actttggttt tactcatgat
    32161 ccaactgctt tgtgttgttc gttgataaat gatacgacga aagagattta tgtctttgat
    32221 gaggcgtata aagtcggatt gataaccaaa gaagttgcga agatgataaa ggacaaaggt
    32281 tatcatcgct cacaaatcat tgctgatagc gcagagtcac ggctgattga agagctcagg
    32341 tcagaacatg gcatatctag aataaaagag agtcggaaag gtaaggatag tattatggca
    32401 ggcgtatcca aattgcaagg atacgctatt tatgtgcatc cagattgtaa aaacatcatg
    32461 gatgaatttt atagttactg ctaccagcga gataaagaag gcaactggtt gaataaacca
    32521 gaggataaaa acaaccactt gatggacgct ttgcgttaca gccttcaatg tatcgaaggt
    32581 ggaaaagcaa ccgtccgcag acgttctgat tatggtctat agagaggaaa gacatgtacc
    32641 aatatttaac ctatccacgg gatggatatg atgagggttc tttgaagaaa gacctgattt
    32701 acaaattgat aacgatacat agcactgaag gctcgcattt gaagaagctt aaaagctact
    32761 atttgggtga gcatgctatc ttagaacaca cgagacgcaa cgtgaacgca cctaattaca
    32821 agacggtagc caatcatgcc aaggatatcg cagacacggc tacgggctat tttatgggca
    32881 atcctatcaa gtataacaat actgctgacg gtgatatcga tgaactactt acagcctttg
    32941 atggtgctga gattgaccaa gtagatgctc agaatgcttt gaacatggct atctatggtc
    33001 gtgcttacga gtacatctat gctaaagagg gtatggctga gttggattca actagtattg
    33061 atccggagaa tactttcatg gtctacgatg atagtattga gcggaagcct ttgtttgcgg
    33121 tctattacta tgaagtaaaa gacgatacga aagacactac caagcaccag gctgagatct
    33181 ttaccgaaaa tctgcactat cacatggtgc tgagaagtac agattcagga acaactcaga
    33241 gcgaggaggc aatacctcac aaccttggtc aaatcccaat tatcgagtat cgcaacaatc
    33301 actttgcaat tggtgactat gagcaacaaa ttagcttgat agacgcttat aattccttga
    33361 tggggaatcg tgtcaatgat aaggaacagg ctgtagagtc tat
//
LOCUS       DAVMAF010000017        32436 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMAF010000017 DAVMAF010000000
VERSION     DAVMAF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.017"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            19..6435
                     /locus_tag="VYO97_000797"
     CDS             19..6435
                     /locus_tag="VYO97_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1753624.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPHEVVLADTSNSEDALSISDKEKV
                     AENKEKHENIHSAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKEDSNQS
                     QGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTYDRIFN
                     GSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFI
                     RGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDASSNFTKIHRIKFADQADEKGMISYY
                     LVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGEASLEI
                     NKTISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAYDDGSA
                     LQYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIKATKY
                     DSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKETKAEYP
                     YVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVT
                     TYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMS
                     ALDGFNIIRYQVFTFKMNDKGEAVDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAI
                     KEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNE
                     SVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQ
                     TGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDG
                     EIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSEL
                     KPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVI
                     NLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
     gene            7067..7543
                     /locus_tag="VYO97_000798"
     CDS             7067..7543
                     /locus_tag="VYO97_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753625.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7581..7886
                     /locus_tag="VYO97_000799"
     CDS             7581..7886
                     /locus_tag="VYO97_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1753626.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            7934..9409
                     /locus_tag="VYO97_000800"
     CDS             7934..9409
                     /locus_tag="VYO97_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1753627.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            9432..9509
                     /locus_tag="VYO97_000801"
                     /pseudo
     CDS             9432..9509
                     /locus_tag="VYO97_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9714..16412
                     /locus_tag="VYO97_000802"
     CDS             9714..16412
                     /locus_tag="VYO97_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1753628.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGIYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESDKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSL
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTNLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHTTLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVVVRKGMLELHKPGAVTLNAEYEGATGQITLTIQVNTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKASAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDFVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEVVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAGLALLGLSAGLVVTKGKKED"
     gene            complement(16660..17517)
                     /locus_tag="VYO97_000803"
                     /pseudo
     CDS             complement(16660..17517)
                     /locus_tag="VYO97_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17727..18086)
                     /locus_tag="VYO97_000804"
     CDS             complement(17727..18086)
                     /locus_tag="VYO97_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1753629.1"
                     /translation="MGMILMKLASILLLILTLVICIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            18178..19134
                     /locus_tag="VYO97_000805"
                     /pseudo
     CDS             18178..19134
                     /locus_tag="VYO97_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            19118..19675
                     /locus_tag="VYO97_000806"
     CDS             19118..19675
                     /locus_tag="VYO97_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1753630.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            19668..19922
                     /locus_tag="VYO97_000807"
     CDS             19668..19922
                     /locus_tag="VYO97_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753631.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            19934..21988
                     /locus_tag="VYO97_000808"
     CDS             19934..21988
                     /locus_tag="VYO97_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1753632.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYSRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            22167..23027
                     /locus_tag="VYO97_000809"
     CDS             22167..23027
                     /locus_tag="VYO97_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1753633.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQILFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            23238..23948
                     /gene="ccdA2"
                     /locus_tag="VYO97_000810"
     CDS             23238..23948
                     /gene="ccdA2"
                     /locus_tag="VYO97_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1753634.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKELYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            23968..24534
                     /locus_tag="VYO97_000811"
     CDS             23968..24534
                     /locus_tag="VYO97_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1753635.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            24545..25657
                     /gene="msrB"
                     /locus_tag="VYO97_000812"
     CDS             24545..25657
                     /gene="msrB"
                     /locus_tag="VYO97_000812"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1753636.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            25880..26617
                     /locus_tag="VYO97_000813"
     CDS             25880..26617
                     /locus_tag="VYO97_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753637.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            26614..28305
                     /locus_tag="VYO97_000814"
     CDS             26614..28305
                     /locus_tag="VYO97_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1753638.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VTFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            28421..28984
                     /locus_tag="VYO97_000815"
     CDS             28421..28984
                     /locus_tag="VYO97_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1753639.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILSSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            29006..>32436
                     /locus_tag="VYO97_000816"
     CDS             29006..>32436
                     /locus_tag="VYO97_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1753640.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKTEESPKEEPKSEIK
                     PTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEESKVEPP
                     VEQAKGPEQPVQPTQAEQPRIPKDSSQPEDPKEDRGAEETPKQEDTPKQEDTQPEVVE
                     TKDEAANQPVEEPKVETPAVEKQTEPKVEQVGEPVEPSEDEKAPVSPEKQPEAPEEKA
                     VEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNDLGPVLETAKGV
                     YASEPVKQPEVNSETNKLKTAIDALNVDKSELQEQLRVAEQKQQADYSAKTWREFKIA
                     ELQAKEINNQTTPLPKQSEIDAATKALQDALQALAVDKTVLQNAINTANSKREEEYTA
                     QTWKALEDALTAVNPVNEDETATQSKVDEATRNLEEAINNLVLLTEKPVLTFIETDKK
                     ALEREVVAKYSLENQNKTKIKSITATLKKGETVVSTVELIGDDVTNETITSAFKNLEY
                     YKEYTLSTTMVYDRGDGDVTEILDNQPIQLDLKKVELKNIKRTDLIKYENGKETNESL
                     ITTVPDDKRNYYLKITSKNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNK
                     FEEEYVHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYIT
                     KEFTGKLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTM
                     KGSSVITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAG
                     TNYRGIVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVS
                     NPVEVGAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGY
                     SSGNRSFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQ"
BASE COUNT        10677 a         5715 c         6906 g         9138 t
ORIGIN      
        1 aatatgtaag gaattaaaat gaaaaaaagt acagtattgt cattaaccac agctgcagtt
       61 attttagcag cctatgcccc tcatgaggta gtcttagcag acacatctaa ctctgaagat
      121 gctttaagca tctctgataa agaaaaagta gcagaaaata aagagaaaca tgaaaatatc
      181 catagtgcta tagaaacttc acaggatttt aaagagaaga aaacagcagt cattgaggga
      241 aaagaagttg ttagtaaaaa tcctgtgaca gacaataaca ctagcaatga agaagcaaga
      301 atcaaagaag attccaatca atcccaagga gatcatacgg actcatttgt gaataaaaac
      361 acagaaaatc ccaaaaaaga agataaagtt gtctatattg ctgaatttaa agataaagaa
      421 tctggagaaa aagcaatcaa gcaactatcc aatcttaaga atacaaaagt tttatatact
      481 tatgatagaa tttttaacgg tagtgccata gaaacaactc cagataactt ggacaaaatt
      541 aaacaaatag aaggtattac atcggttgaa agggcacaaa aagtccaacc catgatgaat
      601 catgccagaa aggaaattgg agttgaggaa gctattgatt acctaaagtc tatcaatgct
      661 ccgtttggga aaaattttga tggtagaggt atggtcattt caaatatcga tactggaaca
      721 gattatagac ataaggctat gagaatcgat gatgatgcca aagcctcaat gagatttaaa
      781 aaagaagact taaaaggaac tgataaaaat tattggttga gtgataaaat ccctcatgcg
      841 ttcaattatt ataatggtgg caaaatcact gtagaaaaat atgatgatgg aagggattat
      901 tttgacccac atgggatgca tattgcaggg attcttgctg gaaatgatac tgaacaagat
      961 atcaaaaact ttaacggcat agatggaatt gcacctaatg cacaaatttt ctcttacaaa
     1021 atgtattctg acgcaggatc tgggtttgcg ggtgatgaaa caatgtttca tgctattgaa
     1081 gattctatca aacacaacgt tgatgttgtt tcggtatcat ctggttttac aggaacaggt
     1141 cttgtaggtg agaaatattg gcaagctatt cgggcattaa gaaaagcagg cattccaatg
     1201 gttgtcgcta cgggtaacta tgcgacttct gcttcaagtt cttcatggga tttagtagca
     1261 aataatcatc tgaaaatgac cgacactgga aatgtaacac gaactgcagc acatgaggat
     1321 gcgatagcgg tcgcttctgc taaaaatcaa acagttgagt ttgataaagt taacataggt
     1381 ggagaaagtt ttaaatacag aaatataggg gcctttttcg ataagaataa aatcacaaca
     1441 aatgaagatg gaacaaaagc tcctagtaaa ttaaaatttg tatatatagg caaggggcaa
     1501 gaccaagatt tgataggttt ggatcttagg ggcaaaattg cagtaatgga tagaatttat
     1561 acaaaggatt taaaaaatgc ttttaaaaaa gctatggata agggtgcacg cgccattatg
     1621 gttgtaaata ctgtaaatta ctacaataga gataattgga cagagcttcc agctatggga
     1681 tatgaagcgg atgaaggtac taaaagtcaa gtgttttcaa tttcaggaga tgatggtgta
     1741 aagctatgga acatgattaa tcctaataaa aaaactgaag tcaaaagaaa taataaagaa
     1801 gattttaaag ataaattgga gcaatactat ccaattgata tggaaagttt taattccaac
     1861 aaaccgaatg taggtgacga aaaagagatt gactttaagt ttgcacctga cacagacaaa
     1921 gaactctata aagaagatat catcgttcca gcaggatcta catcttgggg gccaagaata
     1981 gatttacttt taaaacccga tgtttcagca cctggtaaaa atattaaatc cacgcttaat
     2041 gttattaatg gcaaatcaac ttatggctat atgtcaggaa ctagtatggc gactccaatc
     2101 gtggcagctt ctactgtttt gattagaccg aaattaaagg aaatgcttga aagacctgta
     2161 ttgaaaaatc ttaagggaga tgacaaaata gatcttacaa gtcttacaaa aattgcccta
     2221 caaaatactg cgcgacctat gatggatgca acttcttgga aagaaaaaag tcaatacttt
     2281 gcatcaccta gacaacaggg agcaggccta attaatgtgg ccaatgcttt gagaaatgaa
     2341 gttgtagcaa ctttcaaaaa cacggattct aaaggtttgg taaactcata tggttccatt
     2401 tctcttaaag aaataaaagg tgataaaaaa tactttacaa tcaagcttca caatacatca
     2461 aacagacctt tgacttttaa agtttcagca tcagcgataa ctacagattc tctaactgac
     2521 agattaaaac ttgatgaaac atataaagat gaaaaatctc cagatggtaa gcaaattgtt
     2581 ccagaaattc acccagaaaa agtcaaagga gcaaatatca catttgagca tgatactttc
     2641 actataggcg caaattctag ctttgatttg aatgcggtta taaatgttgg agaggccaaa
     2701 aacaaaaata aatttgtaga atcatttatt cattttgagt cagtggaaga aatggaagct
     2761 ctaaactcca acgggaagaa aataaacttc caaccttctt tgtcgatgcc tctaatggga
     2821 tttgctggga attggaacca cgaaccaatc cttgataaat gggcttggga agaagggtca
     2881 agatcaaaaa cactgggagg ttatgatgat gatggtaaac cgaaaattcc aggaacctta
     2941 aataagggaa ttggtggaga acatggtata gataaattta atccagcagg agttatacaa
     3001 aatagaaaag ataaaaatac aacatccctg gatcaaaatc cagaattatt tgctttcaat
     3061 aaccaaggga tcaacgctcc atcatcaagt ggttctaaga ttgctaacat ttatccttta
     3121 gattcaaatg gaaatcctca agatgctcaa cttgaaagag gattaacacc ttctccactt
     3181 gtattaagaa gtgcagaaga aggattgatt tcaatagtaa atacaaataa agagggagaa
     3241 aatcaaagag acttaaaagt catttcgaga gaacacttta ttagaggaat tttaaattct
     3301 aaaagcaatg atgcaaaggg aatcaaatca tctaaactaa aagtttgggg tgacttgaag
     3361 tgggatggac tcatctataa tcctagaggt agagaagaaa atgcaccaga aagtaaggat
     3421 aatcaagatc ctgctactaa gataagaggt caatttgaac cgattgcgga aggtcaatat
     3481 ttctataaat ttaaatatag attaactaaa gattacccat ggcaggtttc ctatattcct
     3541 gtaaaaattg ataacaccgc ccctaagatt gtttcggttg atttttcaaa tcctgaaaaa
     3601 attaagttga ttacaaagga tacttatcat aaggtaaaag atcagtataa gaatgaaacg
     3661 ctatttgcga gagatcaaaa agaacatcct gaaaaatttg acgagattgc gaacgaagtt
     3721 tggtatgctg gcgccgctct tgttaatgaa gatggagagg ttgaaaaaaa tcttgaagta
     3781 acttacgcag gtgagggtca aggaagaaat agaaaacttg acaaagacgg aaataccatt
     3841 tatgaaatta aaggtgcagg agatttaaga ggaaaaatca ttgaagtcat tgcattagat
     3901 gcttctagca atttcacaaa gattcataga attaaatttg ctgatcaggc tgatgaaaag
     3961 gggatgattt cttattacct agtagatcct gacaaagatg cttctaaata tgaaaaactt
     4021 ggtgaaattt ctgaagataa actcaaaaat gcaaagagcc cagaggaaaa taccaataat
     4081 aatcaagcta aggatgaaga ttcaaaacca gatgaaaaaa gttcagttga gggagaagct
     4141 agccttgaaa taaataaaac tatttcaaca attagagact ttgaaaataa agacctaaag
     4201 aaactcatta aaaagaaatt tagagaagtt gatgatttta caagtgaaac tggtaagaga
     4261 atagaggaat acgattataa atacgatgat aagggaaata ttattgctta tgacgatggt
     4321 agtgccttac aatatgaaac tgaaaaattt gacgaaataa aatcaaaaat ttatggtgtt
     4381 ctaagcccat ctaaagatgg acactttgaa attcttggaa agataagcaa tgtttctaaa
     4441 aatgccaagg tatattatgg aaatagctat aaatcgatag aaatcaaagc gactaagtat
     4501 gattcacatt caaaaacgat gatatttgat ttatacgcta atattaatga tattgtagat
     4561 ggattagctt ttgctggaga tatgagatta tttgttaaag atgacaatca aataaaagct
     4621 gaaactaaaa ttagaatgcc tgaaaaaaat aaggaaacta aagcagaata tccctatgta
     4681 tcaagttatg ggaatgtaat cgaattagga gaaggagatc tttcaaagaa caaaccagac
     4741 aatttaacta aaatggaatc tggtaaaatc tattctgatt cagaaaaaca acaatatctg
     4801 ttaaaggata acatcattct aagaaaaggc tatgcactaa aagtgactac ctataatcct
     4861 ggaaaaacgg atatgttaga aggaaatgga gtctatagca aggaagatat agcaaaaata
     4921 caaaaggcca atcctaatct aagagtcctt tcagaaacaa caatttatgc tgatagtaga
     4981 aatgttgaag atggaagaag tacccaagct gtattaatgt cggctttgga cggctttaac
     5041 attataaggt atcaagtgtt tacatttaaa atgaacgata aaggggaagc tgtcgataaa
     5101 gacggaaatc ttgtgacaga ttcttctaaa cttgtattat ttggtaagga tgataaagaa
     5161 tacactggag aggataagtc caatgtagaa gctataaaag aagatggctc catgttattt
     5221 attgatacca aaccagtaaa cctttcaatg gataagaact actttaatcc atctaaatct
     5281 aataaaattt atgtacgaaa tccagaattt tatttaagag gtaagatttc tgataagggt
     5341 ggttttaact gggaattgag agttaatgaa tcggttgtag ataattattt aatctacgga
     5401 gatttacaca ttgataacac tagagatttt aatattaagc tgaatgttaa agacggtgac
     5461 atcatggact ggggaatgaa agactataaa gcaaacggat ttccagataa ggtaacagat
     5521 atggatggaa atgtttatct tcaaactggc tatagcgatt tgaatgctaa agcagttgga
     5581 gtccactatc agtttttata tgataatgtt aaacccgaag taaacattga tcctaaggga
     5641 aatactagta tcgaatatgc tgatggaaaa tctgtagtct ttaacatcaa tgataaaaga
     5701 aataatggat tcgatggtga gattcaagaa caacatattt atgtaaatgg aaaagaatat
     5761 acatcatttg atgatattaa acaaataaca gacaagacac taaacattaa gattgttgta
     5821 aaagattttg caagaaatac aaccgtaaaa gaattcattt taaataaaga tacgggagag
     5881 gtaagtgaat taaaacctca tagggtaact gtgaccattc aaaatggaaa agaaatgagt
     5941 tcaacgatag tgtcggaaga agattttatt ttacctgttt ataagggtga attagaaaaa
     6001 ggataccaat ttgatggttg ggaaatttct ggtttcgaag gtaaaaaaga cgctggctat
     6061 gttattaatc tatcaaaaga tacctttata aaacctgtat tcaagaaaat agaggagaaa
     6121 aaggaggaag aaaataaacc tacttttgat gtatcgaaaa agaaagataa cccacaagta
     6181 aaccatagtc aattaaatga aagtcacaga aaagaggatt tacaaagaga agatcattca
     6241 caaaaatctg attcaactaa ggatgttaca gctacagttc ttgataaaaa caatatcagt
     6301 agtaaatcaa ctactaacaa tcctaataag ttgccaaaaa ctggaacagc aagcggagcc
     6361 cagacactat tagctgccgg aataatgttt atagtaggaa tttttcttgg attgaagaaa
     6421 aaaaatcaag attaagacaa aagctataga aaaaaatggt ttatgtactg agattagata
     6481 gtgaggtgat gacatagttt tgtgaaaacg gccatttata actcaattat ttagtttact
     6541 tcacttcact attgatacta tttggagtta ttaatggact tagtttagat aactaatgaa
     6601 ttgattgaaa gggttagtat tgacaatatt ggtcatattg attagaaaat agagtctatc
     6661 aaaatttaaa ggctaataga ggtgatgaga caatttcaag gaattaagta gcgtttggtt
     6721 gcttaattcc tttttatttg gaaggaagtt tttcgtaacc gcccaataac gaagtctatt
     6781 ggacggttac gatatgatca ctgacttcgt cagtcttatc tacaacctca aaactgtgtt
     6841 ttgagcaacc tgcggctagc ttcctagttt actctttgat tttcattgag tattagaaca
     6901 gaaaaaatgc ttggagtatt tgtttgtgtg tttattttta tataacaaac tataaacaaa
     6961 acaaaaatat aaaaaaagag acaaaaaaga acaaaaagta attgacaacg gtttcatata
     7021 gtgttatact tatggcataa agttaatgga aagaagggaa aaacttatgg tattagatta
     7081 tttctttgac aaaaaccttg tgttttgctt agaagcggat aatcaagaac atctctttga
     7141 tcaggttgca actttattgg aagaacgaga aattgtaact ccaacttatc gtgaagcctt
     7201 gatcacgcgt gaaaagtcat ttccaactgg tttagatatg gaatttctag gaaaggactt
     7261 gccaaatgta gcgattcctc acacagatat tgttcataat ctagctgaga aagtggtggt
     7321 tgttcgatta gaaaaaccag taacttttca caatatgata gctcctgata aggaagtaga
     7381 agtatctcta ctcttcttta tcattaacaa ttcaagttca agtcaaacga acattctggc
     7441 tcagttgatg gactttttca caggaaatgg tcatcttgaa gacctatcaa aaatttctga
     7501 accagaaaaa ctttatgctt acattgctga agcaaccgct taatcttgtc tattaaaaaa
     7561 taaaatcgga ggaaatccaa atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact
     7621 caagtcacca aattaaaagt gctctagaag aagaactttc aaaccgtggt tatgatgttc
     7681 actgtgatgc agtcatggtg aaagatgtaa acgaagacct tatgaaaggt tacgatatct
     7741 ttacaccaat cgctgcaaca gaccttggtt ttgaaccagg tatcccagtt atcgaagctg
     7801 ggccaatctt attccgtatc ccagcaatga gcgctccagt atttgacaat attgaagcag
     7861 ctattaaaga acatggatta agctaattct aatttgttaa cattcatata acataaaaaa
     7921 gggaggaact gttatggatg caatctttga cctaatcgga aaggttttca atcccatctt
     7981 agaaatgggt ggacctgtca tcatgttgat cattttgaca gtattggctt tactttttgg
     8041 agtgaaattc tccaaagcgc ttgaaggtgg tatcaaactt gccatcgctc ttacaggtat
     8101 cggtgctatc atcggtatgc taaatggtgc tttctcagca tcacttgcaa aattcgttga
     8161 aaacactggt atccaattga gtattacaga cgttggttgg gcaccactcg caacaatcac
     8221 ttggggttct gcttggacac tatacttctt gctcatcatg ttgattgtca acgtagtgat
     8281 gcttgctatg aagaaaaccg atacacttga tgtcgatatc tttgatatct ggcacttgtc
     8341 tatcacaggt ctcttgatta aatggtatgc tgataacaat ggtgtgagtc aaggggtttc
     8401 actctttatt gctacagcag ctatcgtcct tgtcggtgtg ttgaaaatta tcaactctga
     8461 cttgatgaaa cctacatttg atgaccttct taacgcccca agttcatcac caatgacatc
     8521 aactcacatg aactacatga tgaacccagt tatcatggtt ttggataaga tttttgaaaa
     8581 attcttccca ggccttgata aatatgactt tgatgctgct aaattgaaca agaaaatcgg
     8641 tttctgggga tctaaattct tcatcggttt catccttggt atcgttatcg gtattatggg
     8701 aactccacat ccaattgcag gtgttgcaga tgcagataaa tggcgtcttg ttatcaaagg
     8761 atggttgtct cttggtttga ctgccggtgt atctttggaa ctcttctcac ttatcggttc
     8821 atggttcatc gcagccgtag aaccactatc acaaggtatt acaaacgttg ctactaaacg
     8881 tcttcaagga cgtaaattca atatcggtct tgactggcca ttcatcgctg gtcgtgctga
     8941 aatctgggct tgtgccaacg tacttgcacc aatcatgttg attgaagcag tgcttctttc
     9001 aaaagttgga aatggtatct tgccacttgc aggtatcatc gctatgggtg ttactccagc
     9061 tctcttggtt gtaactcgtg gtaaattgct ccgtatgatt atcttcggaa cactcttgtt
     9121 gccactcttc cttctttcag gtacccttat tgcaccattt gcaacagaac ttgctaaagg
     9181 tgtaggtgcc ttcccagaag gtgtgagcca aactcaattg attactcact ctactcttga
     9241 aggaccaatc gaaaaacttc ttggttggac aattggtaac actacaactg gtgatatcaa
     9301 agcaatcctt ggtgcagtag tcttccttgt attctatatc ggtatctttg cttggtacag
     9361 aaaacaaatg atcaaacgta acgaagagta cgcagcaaaa gcaaaataat gcgctcctat
     9421 aaaatataaa ctcaatgaaa atcaaagagc aaactaggaa actagccaca ggttgctcaa
     9481 aacacagttt tgaggttgta gataagactg gcgaagtcag ctcaaaacat ggttttgagg
     9541 ttatagatga aactgacgaa gtcagtaatc atacatacgg caaggcgaca ctgacgaagt
     9601 ttgaattgga ttttcgcaga gtgtaaattg ttgaaactag gttgtcatac catgtatacc
     9661 acttgggctt tggtagtagc taactgcact aaatataata taaggagggt tttatgggga
     9721 aaggccattg gaatcggaaa agagtttata gcattcgtaa gtttgctgtg ggagcttgct
     9781 cagtaatgat tgggacttgt gcagttctat taggaggaaa tatagctgga gaatctgtag
     9841 tttatgcgga tgaaacactt attactcata ctgctgagaa acctaaagag gaaaaaatga
     9901 tagtagaaga aaaggctgat aaagctttgg aaactaaaaa tgtagttgaa aggacagaac
     9961 aaagtgaacc tagttcaact gaggctattg catctgagaa gaaagaagat gaagccgtaa
    10021 ctccaaaaga ggaaaaagtg tctgctaaac cggaagaaaa agctccaagg atagaatcac
    10081 aagcttcaaa tcaagaaaaa ccgctcaagg aagatgctaa agctgtaaca aatgaagaag
    10141 tgaatcaaat gattgaagac aggaaagtgg attttaatca aaattggtat tttaaactca
    10201 atgcaaattc taaggaagcc attaaacctg atgcagacgt atctacgtgg aaaaaattag
    10261 atttaccgta tgactggagt atctttaacg atttcgatca tgaatctcct gcacaaaatg
    10321 aaggtggaca gctcaacggt ggggaagctt ggtatcgcaa gactttcaaa ctagatgaaa
    10381 aagacctcaa gaaaaatgtt cgccttactt ttgatggcgt ctacatggat tctcaagttt
    10441 atgtcaatgg tcagttagtg gggcattatc caaatggtta taaccagttc tcatacgata
    10501 tcaccaaata ccttcacaaa gatggtcgtg agaatgtgat tgctgtccat gcagtcaaca
    10561 aacagccaag tagccgttgg tattcaggaa gtggtatcta tcgtgatgtg actttacaag
    10621 tgacagataa ggtgcatgtt gagaaaaatg ggacaactat tttaacacca aaacttgaag
    10681 aacaacaaca tggcaaggtt gaaactcatg tgaccagcaa aatcgtcaat acggacgaca
    10741 aagaccatga acttgtagcc gaatatcaaa tcgttgaacg aggtggtcat gctgtaacag
    10801 gcttagttcg tacagcgagt cgtaccttaa aagcacatga atcaacaagc ctagatgcga
    10861 ttttagaagt tgaaagacca aaactctgga ccgttttaaa tgacaaacct gccttgtacg
    10921 aattgattac gcgtgtttac cgtgacggtc aattggttga tgctaagaag gatttgtttg
    10981 gttaccgtta ctatcactgg actccaaatg aaggtttctc tttgaatggt gaacgtatta
    11041 aattccatgg agtatccttg caccacgacc atggggcgct tggagcagaa gaaaattata
    11101 aagcagaata tcgccgtctc aaacaaatga aggagatggg agttaactcc atccgtacaa
    11161 cccacaaccc tgctagtgag caaaccttgc aaatcgcagc agaactaggt ttactcgttc
    11221 aggaagaggc ttttgatact tggtatggtg gcaagaaacc ttatgactat ggacgttttt
    11281 ttgaaaaaga tgccactcac ccagaagctc gaaaaggtga aaaatggtct gattttgacc
    11341 tacgtaccat ggtcgaaaga ggcaaaaaca accctgctat cttcatgtgg tcaattggta
    11401 atgaaatagg tgaagctaat ggtgatgccc actctttagc aactgttaaa cgtttggtca
    11461 aggttatcaa ggatgttgat aagactcgct atgttaccat gggagcagat aaattccgtt
    11521 tcggtaatgg tagcggaggg catgagaaaa ttgctgatga actcgatgct gttggattta
    11581 actattctga agataattac aaagccctta gagctaagca tccaaaatgg ttgatttatg
    11641 gatcagaaac atcttcagct acccgtacac gtggaattta ctatcgccct gaacgtgaat
    11701 tgaaacatag caatggacct gagcgtaatt atgaacagtc tgactatggg aatgatcgtg
    11761 ttggttgggg gaaaacagca accgcttcat ggacttttga ccgtgacaac gctggctatg
    11821 ctggacagtt tatctggaca ggtacggact atattggtga acctacacca tggcataacc
    11881 aaaatcaaac tcctgttaag agctcttact ttggtatcgt agatacagcc ggcattccaa
    11941 aacatgactt ctacctctac caaagccaat gggtttctgt taaaaagaaa ccgatggtac
    12001 accttcttcc tcactggaac tgggaaaaca aagaattagc atccaaagta gctgactcag
    12061 aaggtaagat tccagttcgt gcttattcga atgcttctag tgtagaatta ttcttgaatg
    12121 gaaaatctct tggtcttaag actttcaata aaaaacaaac cagcgatggg cggacttacc
    12181 aagaaggtgc aaatgctaat gaactttatc ttgaatggaa agttgcctat caaccgggta
    12241 ccttggaagc aattgctcgt gatgaatctg acaaggaaat tgctcgagat aagattacga
    12301 ctgctggtaa gccagcggca gttcgtctta ttaaggaaga ccatgcgatt gcagcagatg
    12361 gaaaagactt gacttacatc tactatgaaa ttgttgacag ccaggggaat gtggttccaa
    12421 ctgctaataa tctggttcgc ttccaattgc atggccaagg tcaactggtc ggtgtagata
    12481 acggagaaca agccagccgt gaacgctata aggcgcaagc agatggttct tggattcgta
    12541 aagcatttaa tggtaaaggt gttgccattg tcaaatcaac tgaacaagca gggaaattca
    12601 cccttactgc ccactctgat ctcttgaaat cgaaccaagt cactgtcttt actggtaaga
    12661 aagaaggaca agaaaagact gttttgggga cagaggtgcc aaaagtacag accattattg
    12721 gagaggcacc tgaaatgcct accactgttc cgtttgtata cagtgatggt agtcgtgcag
    12781 aacgtcctgt aacctggtct ttagtagatg tgagcaagcc tggtattgta acggtgaaag
    12841 gtatggctga cggacgagaa gtagaagctc gtgtagaagt gattgctctt aaatcagagc
    12901 taccagttgt gaaacgtatt gctccaaata ctaacttgaa ttctgtagac aaatctgttt
    12961 cctatgtttt gactgatgga agtgtacaag agtatgaagt ggacaagtgg gagattgccg
    13021 aagaagataa agctaagtta gcaattccag gttctcgtat tcaagcgacc ggttatttag
    13081 aaggtcaacc aattcataca acccttgtgg tagaagaagg caatcctgca gcacctgtag
    13141 tgccaactgt tactgttgga ggtgaagcag taacaggtct tactagtcga caaccaatgc
    13201 aatatcgtac tctatcttat ggtgcccaat tgccagaagt cacagcaagt gctgaaaatg
    13261 ctgatgtgac agttcttcaa gcaagcgcag caaacggcat gcgtgcaagc atatttgttc
    13321 agcctaaaga tggtggccct cttcaaacct atgcaattca attcctagaa gaagcaccaa
    13381 aaattgctca cttgagcttg caagtggaaa aagctgacag tctcaaagaa gaccaaactg
    13441 tcaaattgtc ggttcgagct cactatcaag atggaacgca agctgtatta ccagctgata
    13501 aagtaacctt ctctacaagt ggtgaagggg aagttgtagt ccgaaaagga atgcttgagt
    13561 tgcataaacc aggagcagtc actctgaacg ctgaatatga gggtgctaca ggtcaaatca
    13621 ctctcactat ccaagtcaat actgagaaga agattgcgca atccatccgt cctgtaaatg
    13681 tagtgacaga tttgcatcag gaaccaagtc ttccagcaac agtaacagtt gagtatgaca
    13741 aaggtttccc taaaactcat aaagtcactt ggcaagctat tccgaaagaa aaactagact
    13801 cctatcaaac atttgaagta ctaggtaaag ttgaaggaat tgaccttgaa gcgcgtgcaa
    13861 aagtctctgt agaaggtatc gtttcagttg aagaagtcag tgtgacaact ccaatcgcag
    13921 aagcaccaca attaccagaa agtgttcgga catatgattc aaatggtcac gtttcatcag
    13981 ctaaggttgc atgggatgcg attcgtccag agcaatacgc taaggaaggt gtctttacag
    14041 ttaatggtcg cttagaaggt acgcaattaa caactaaact tcatgttcgc gtatctgctc
    14101 aaactgagca aggtgcaaac atttctgacc aatggaccgg ttcagaattg ccacttgcct
    14161 ttgcttcaga ctcaaatcca agcgacccag tttcaaatgt taatgacaag ctcatttcct
    14221 acaataacca accagccaat cgttggacaa actggaatcg tagtaatcca gaagcttcag
    14281 tcggtgtcct attcggagat tcaggtattt tgagcaaacg ctccgttgat aatctaagtg
    14341 tcggattcca cgaagaccat ggagttggtg caccgaagtc ttatgtgatt gagtattatg
    14401 ttggtaagac tgtcccaaca gctcctaaaa accctagttt tgttggtaat gaggaccatg
    14461 tctttaatga ttctgccaac tggaaaccag ttactaatct aaaagcctct gctcaactca
    14521 aggctggaga aatgaaccac tttagctttg ataaagttga aacctatgct gttcgtattc
    14581 gcatggtgag agcagacaac aaactaggaa cgtctatcac agaggtacaa atctttgcga
    14641 aacaagttgc ggcagccaag caaggacaaa caagaatcca agttgacggt aaagacttag
    14701 caaacttcaa ccctgatttg acagactact accttgagtc tgtagatgga aaagttacgg
    14761 cagtcacagc aagtgttagc aacaatggcc tcgctaccgt cgttccaagc gttcgtgaag
    14821 gtgagccggt tcgtgtcatc gcgaaagctg aaaatggcga catcttagga gaataccgtc
    14881 tgcacttcac taaggataag aacttacttt ctcataaacc agttgctgcg gttaaacaag
    14941 ctcgcttgct acaagtaggt caagcacttg aattgccgac taaggttcca gtttacttca
    15001 caggtaaaga cggctacgaa acaaaagatt tgtcagttga atgggaagaa gttccagcag
    15061 aaaatctgac aaaagcaggt caatttaccg ttcgaggcca tgtccttggt agtgattttg
    15121 ttgctgaggt cactgtacga gtgacagaca aactaggcga agctctttca gataacccta
    15181 actatgatga aaacagtaac caggcctttg cttcagcaac caatgatatt gacaaaaact
    15241 ctcatgaccg cgttgactat ctcaatgacg gagatcattc agaaaatcgt cgttggacaa
    15301 actggtctcc aacaccatct tctaatccag aagtatcagc gggtgtgatc ttccgtgaaa
    15361 atggtaagat tgtagaacgg actgttgctc aagccaaact tcacttcttt gcagatagtg
    15421 gtacggatgc accaactaaa ctcgttttag aacgctatgt cggtccagag tttgaagtgc
    15481 caacctacta ttcaaactac caagcctacg acgcagacca tccattcaac aatccagaaa
    15541 attgggaagt tgtgccttat cgtgcggata aagacatcgc agctggtgat gaaatcaacg
    15601 taacatttaa agctgtcaaa gccaaagcta tgagatggcg tatggagcgt aaagctgaca
    15661 agagcggtgt tgcgatgatt gagatgacct tccttgcacc gagtgaattg cctcaagaaa
    15721 gtacgcaatc gaagattctt gtagatggaa aagaacttgc tgatttcgct gaaaatcgtc
    15781 aagactatca aattacctat aaaggtcaac ggccaaaagt ctcagttgaa gaaaacaatc
    15841 aagtagcttc aactgtggta gatagtggag aagatagcct tccagtactt gttcgcctcg
    15901 tttcagaaag tggaaaacaa gtcaaggaat accgtatcca cttgactaag gaaaaaccag
    15961 tttctgagaa gacagttgct gctgtacaag aagatcttcc aaaactcgaa tttgttgaaa
    16021 aagatttggc atacaagaca gttgagaaaa aagattcaac actgtatcta ggtgaaactc
    16081 gtgtagaaca agaaggaaaa actggtaaag aacgtatctt tacagcgatt aatcctgatg
    16141 gaagtaagga agaaaaactc cgtgaagtgg tagaagctcc gacagaccgc atcgtcttgg
    16201 ttggaaccaa accagtagct caagaagcta aaaaaccaca agtgtcagaa aaagcagata
    16261 caaaaccaat tgattcaagt gaagctagtc aaactaataa agcccagtta ccaaatacag
    16321 gtagtgcggc aagccaagca gcagtagcag gtttagctct tctaggtttg agtgcaggat
    16381 tagtagttac taaaggtaaa aaagaagact agaaattttg ataccttctt tatcattaaa
    16441 gaccaaagaa aaactcttgt cctaaataga agataaagag tctgggacaa aatagttctc
    16501 aaccacaaaa aagctagata tttccaattg cggaactcta gctttttaat tttgagtcgt
    16561 tctcttattg tatagtagat tgaaactaga gtagtacact tctacttcta aaacattgtt
    16621 agaaatcgat ttgactgtcc ttatcgattt gtcctgttct tatttcattt cattatgttt
    16681 taggaggtta ttggccataa gtaccaatcc catgtcaatt ctcacttgac gcttgcctct
    16741 cagattacat ctcttgtaac ccaaacaagc ctttaactgc ccaaagacag gttccacatc
    16801 aatcttgcgt tgagcgaaaa tctgtctacc ttggggagat aaaagcgcct gacattcttt
    16861 agctttcaag ttttgatagc gttcgttcat atatagcccc cttttgaggg gctgattcag
    16921 gttcgtcggc atagtaaacc ttgatttcct gttgaaagtc tgtctgtgtt ttctgatgtt
    16981 tggtatggtg aaaacgataa taccagccat caggatgtgt gtagctatcc tccttgtcat
    17041 tatagtgcca attcgctaag tttttagctg actgtttata ccctctcttt tgttccttat
    17101 caaacatggc atatttaatc agaaggggag tgatttcttc ttgaaaatag acttgtatct
    17161 gttcttgaag tttggcggag aatttctctg tcgttttctt ccacacaaaa ctatacttgt
    17221 tggcattgga ttcaatctta gttccgtcaa taaacaaaca atctaaggtc actaactctt
    17281 ccatttttca atttttcgac cagagaaaat cccttgtgaa tagacaaata gaagagtaga
    17341 tacaagcatt ttagggtgat aagacgggcg atcaaaggca tgatagaagg cgtagaagtg
    17401 atgttcctcc aaggtattca ccactttttc aatagtaaag acgagatggt cttgtggtaa
    17461 gaaggaactg atttctagtg gtaaagttgt ttgatttgtg ttatagtgaa tatgcatggg
    17521 atagcctttc taaatggttt attaagcaat tctattttac caagactatc gtaaccatgc
    17581 aaaaaagcaa cggcaaatcc gttgcctttt tggatataag agactattgt cccatactct
    17641 tttctttgta taaaattgta tagtgttatt gtctatacta ttttgtagag aaagcaaaaa
    17701 tccctagaaa atgcttaggg attcgactat ttcattaaga acaattcaac aatcatttct
    17761 atatacatga taagggttaa taagaatcct gcacgccaga agaatttgat gaatttagga
    17821 tagtaaaagc tgcgtttttt caaaaggaag aaaaagacga gaataatggc taggatcgag
    17881 agggctagcc ccagtctagg gaggaaatta tgggtaaagg ttttagctgt aatcaagtaa
    17941 tactcaaata ccaagactgg aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat
    18001 ctaaaaagtt tggttaggat aatgcagatg actaaggtca gtatcaataa taaaatagat
    18061 gctaatttca ttaaaatcat acccatattg tatcataaaa aaaggctaat ggaaacgaga
    18121 aacagaggaa tttataggaa agtcaggctt ttgagattgt gggtgatttt tgttataatg
    18181 aaagttataa aatcttataa taccttgaat gattattatc gaaaactctt tggagaaaag
    18241 acttttaaag tccctattga tgcgggattt gactgtccca atcgtgatgg aactgtggct
    18301 catggaggct gtactttttg tacggtttca ggttctggag atactattgt agccccagat
    18361 gcgcctatcc gtgagcaatt ttataaggaa attgacttta tgcaccgtaa gtggccggat
    18421 gttcagaagt atctggttta ttttcaaaat tttaccaaca cccatgaaaa ggtggaagtt
    18481 attcgagagc gttatgaaca ggctatcaat gagccaggtg tggtaggaat caatattgga
    18541 atgcgcccag actgtttacc agacgaaacc atcgaatatt tggctgagtt atcggagtgc
    18601 atgcatgtga cggttgaatt gggcttgtag actacttatg aagcaacttc tgacctgatt
    18661 aaccgtgcgt attcttatga attgtaggtg gaaacggtca agcgtttgag aaagtatccc
    18721 aagattgaga ttgtttccca tttgattaat ggtttgcctg gtgaaaccca tgagatgatg
    18781 gttgaaaatg tccgccgttg tgtcacgaat aacgatattc aagggattaa actgcatttg
    18841 ctccatctta tgacaaatac tcgtatgcaa cgagattacc atgagggacg tttacaattg
    18901 atgagtcagg acgaatatgt cagggtcatc tgtgaccaac tggaaatcat tcccaaacat
    18961 atcgtcatcc atcgaatcac aggagataca cctagggata tgctgattgg gcttatgtgg
    19021 agcctcaata aatgggaagc tctcaacagc attaagatgg agatgcgacg tcgtggaagt
    19081 gttcaaggat gtaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc
    19141 acatgatttt ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg
    19201 aaatggtcat gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat
    19261 tcagaagcaa gccttggaaa agacccaaga gcgtttgcat caggctggct tgacaaatgc
    19321 ccagttaatc ttgcaaggcc atgagacact ggaccagttt gtgacaaaag ctaaggcagg
    19381 gatttttaat ctgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac
    19441 aacgattgag gcattagaaa agctatgtgg cttacttgtc aaaggtggac gaattgctat
    19501 tatgatttac tatggtcatg aaggaggcga cctcgagaga gatgctgtct tggattttgt
    19561 gatccagttg aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtcaa
    19621 caacccgccg tttttagtga tgattgaaaa attagagaga tacagacatg gataaacaat
    19681 acctacacga aaagctggat gccatgcgcc agaattttgt tgaatcaact caccacgaac
    19741 gagcggtggg cgtgctagac caagcgcata tgagcaaaaa aatgcttaaa atcaagaaaa
    19801 aattagttgc tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca
    19861 agattgacca aaaaatcaaa gagcagaagg agatatttga atcctgttgt aaaaaagatt
    19921 aaggaggacg tgcgtggaat tactgattta cctcatccta tttttactgg ttttgattgt
    19981 ctcaagtaca accaataagc tcctaccctt tttgcctctc cctttggtgc agattctttt
    20041 gggaattgtg attggtctct ttttacctaa tactgacttt caccttaata cggagttgtt
    20101 tttggcactg gttatcggcc ccttgctttt ccgagaggct gaagaagcag atgttacggc
    20161 tattttaaaa cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac
    20221 cctgagtttg ggtggcttgg cccatcttct ttggttcagc cttcccttgg cagcttgctt
    20281 ggctgttggg gcagcccttg gtcctacgga cttggtggcc tttgcctctc tttcggagcg
    20341 ttttagcttt cctaagcgcg tgtccaatat tcttaagggc gaaggactct tgaatgatgc
    20401 ttctggtttg gtggcttttc aggtagcttt gacagcttgg acaactggag ctttttctct
    20461 ggggcaagct agcagttcgc tcatcttttc aatcctaggc ggttttttaa ttggattttt
    20521 aacagccatg accaaccgct tcctccatac cttcttgcta agtgtgcgcg caacggatat
    20581 tgccagtgaa cttttattag aattgagttt gcctctagtg accttctttc tggcagaaga
    20641 agtccatgtt tcagggatta ttgccgtcgt agttgctgga attttaaagg caagtcgctt
    20701 caagaaaatc acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcatac
    20761 agtgaccttt atgctcaacg gttctgtctt tgtgatttta gggatggagt tggaaatgat
    20821 agcagaacct atcttgacca atccaatcta taatcctcta cttttattgc tatctctcat
    20881 cgcccttacc tttgtcctct ttgtcattcg ttttattatg atctatggct attatgccta
    20941 tagaacccga cgcctcaaga aaaagctaaa taagtatatg aaggacatgt ttctcttgac
    21001 cttttcaggt gttaagggaa cggtgtcgat tgctacgatt ctcttgatac caagtaatct
    21061 agaacaggag tatcctctct tgcttttcct tgttgcaggt gtgacgcttg tcagcttttt
    21121 aacaggtctc ttggtcttgc ctcatctttc tgatgaagag gaagaaagca aggattatct
    21181 catgcatatc gccattttga atgaagtaac gctagagttg gaaaaagagt tggaagacac
    21241 cagaaataaa cttcccctct atgcggctat tgacaattat catggacgta ttgaaaatct
    21301 cattttaagc caagaaaacc aggatgatca agaagattgg gctgctttga aactcttgat
    21361 tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa gaggggaaca ttagcaatcg
    21421 tgcttaccga gtttaccaac gttatctgaa aaatatagaa caaggaatca atcgtaaact
    21481 tgcctcaaga ctgacctatt atttccttgt ttctttgcgg attttacgtt ttctccttca
    21541 tgaagttttt actctcggaa agaccttccg tagctggaag aacaaggagc aaagccgtct
    21601 ccgtgctctt gattatgacc aaattgcaga gctctatctt gccaatacgg agatgattat
    21661 tgaaagtttg gaaaacctga agggagtcta cagccgctct ttgattagtt ttatgcagga
    21721 gtctcgtctt cgagaaacaa ctattattag cagtggtgcc tttgtcgaac gggttatcaa
    21781 tcgtgtcaaa cccaacaata tcaatgaaat gctgagaggc tattatctgg agcgcaagtt
    21841 gattttcgaa tacgaagaaa aacgattgat tacgactaag tatgccaaga aattacgaca
    21901 aaatgtaaat aacttagaga actattcctt gaaggaagct gccaataccc tgccgtatga
    21961 tatggtggaa ttggtaagaa gaaattagtt aatattcttc gaaaatctct tcaaaccacg
    22021 tcagcgtcgc cttaccgtat gtatgtgact gacttcgtca gtcttatcta caacctcaaa
    22081 acagtgtttt gagcagcttg cggctagctt tctagtttgc tctttgattt tcattgagta
    22141 taagattata aatgaaggag tatgacatga aaaagtggtg gaaagagctg atagacaagc
    22201 ctttattaaa agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg
    22261 cagtagccta ctattggttg atttcgattt tccccctgct tttggtggtg gtcaatatcc
    22321 tcccctattt tcagattcct gtgggagaat ttttgggctt tatgaaagac gttctgcccc
    22381 caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt tttgacgcaa ccttcaacag
    22441 gtttattgag tttttctgtt ttatcggcgt tatggagttt ttcaaaatct atgaatttct
    22501 tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc
    22561 agatgctaag tctcctagtc agttttggct tgcagatcct ctttgcgttt gccttgtttt
    22621 tgatcttatt tggtcagatg attttggctt tacttgctca ttattggaca aaagatggca
    22681 ttatctatca ggctttacaa gggttggcag gtcctctgat ttacgccctc ctctttgcta
    22741 tcttggtcat gctttattat tttcttccca atctatctaa caggaaaatt agctataccc
    22801 taccaggcag tgcctttgtt ctcttagtga ttttaggttt actaactctg ttctctagtt
    22861 atctcaatta ctatgtccat catttggtgg atgtccgaat tttaggttcc gttcttcttg
    22921 ttgttatgat gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggttatca
    22981 atgccagtat gcagagtttg aaagatccgg tctttaaaaa agactaacta tttatccatt
    23041 acaaaagcgc atagtatcaa gactttatag aaccttgata ctatgcgctt ttgtgctttg
    23101 acagtttttt gtatagtttt tgtccaagat ttctaaatca tctgattaaa ttcctatttt
    23161 cccctatcat tgtccctgtt ttttcgaaaa tggaaggagt ataataaatc tatcaataaa
    23221 atgataggag gaactttatg ggtcatattt tcttttttct aagtgtcttt ttggcaggga
    23281 ttctatcctt cttttctcct tgtatcttac ctttgttacc ggtctataca ggagtgttac
    23341 tagatgataa ggatggtgct caggcttcta gcggcaaatt ttcaatctca gttactagtt
    23401 tattacgaac tctggccttt atagcaggaa tttcctttat atttattttg ttgggctatg
    23461 gagctggttt tttaggcgat ttgctttatg cttcttggtt ccaatatctt actggggcaa
    23521 ttattatcct tcttggtttg caccaaatgg agattctaca ctttaaggag ctttataagg
    23581 aaaagaggct acaactgcaa ggacaggggc aaaatggtaa gggctatagt caggcatttt
    23641 tattgggctt gacctttagt tttgcttgga cgccttgcgt ggggccggtt ctggggtctg
    23701 ttttggcctt ggcggcttca ggtggttcag gagcttggca gggagctggt ctcatgttgg
    23761 tgtatacgct gggcttggcg ctaccattct tgcttctagc tctgacctct agttatgttt
    23821 tgaaacattt ccgaaaactt catccctatc tcggaatcct caaaaaagtg ggtggttttc
    23881 tcattattgt gatgggactc ttggttctgt ttggaaatgc ttcaatttta agtcaattat
    23941 ttgaataaaa tggaaaggaa tatcaatatg aaaaaatggc aaacatgtgt tcttggagca
    24001 ggttcgctcc tttgtttgac ggcttgttca ggcaagtccg tgactagtga acaccaaacg
    24061 aaagatgaaa tgaagacgga gcagacagct agtaaaacaa gcgcagctaa agggaaagag
    24121 gtggctgatt ttgaattgat gggagtagat ggcaagacct accgtttatc tgattacaag
    24181 ggcaagaaag tctatctcaa attctgggct tcttggtgtt ccatctgtct ggctagtctt
    24241 ccagatacgg atgagattgc taaagaagct ggtgatgact atgtggtctt gacagtagtg
    24301 tcaccaggac ataagggaga gcaatctgaa gcggacttta agaattggta taagggattg
    24361 gattataaaa atctcccagt cctagttgac ccatcaggca aacttttgga aacttatggt
    24421 gtccgttctt acccaaccca agcctttata gacaaagaag gcaagctggt caaaacacat
    24481 ccaggattca tggaaaaaga tgcaattttg caaactttga aggaattagc ctaggaggtg
    24541 tcttatgaat gataagttaa aaatcttctt gttgctagga gtattttttc tagccataac
    24601 cggtttctat gttctattga tacgaaatgc agggcagaca gatgcctcgc aaattgaaaa
    24661 ggcggcagtt agccaaggag gaaaagcagt gaaaaaaaca gaaattagta aagacgcaga
    24721 cttgcacgaa atttatctag ctggaggttg tttctgggga gtggaggaat atttctcacg
    24781 cgttcccggg gtgacggatg ccgtttcagg ctatgcaaat ggtagaggag aaacaaccaa
    24841 gtacgaattg attaaccaaa caggtcatgc agaaaccgtc catgtcacct atgatgccaa
    24901 gcaaatttct ctcaaggaaa tcctgcttca ctatttccgc attatcaatc caaccagcaa
    24961 aaataaacaa ggaaatgatg tggggaccca gtaccgtact ggtgtttatt acacagatga
    25021 caaggatttg gaagtgatta accaagtctt tgatgaggtg gctaagaaat acgatcaacc
    25081 tctagcagtt gaaaaggaaa acttgaagaa ttttgtggtg gctgaggatt accatcaaga
    25141 ctatctcaag aaaaatccaa atggctactg ccatatcaat gttaatcagg cggcctatcc
    25201 tgtcattgat gccagcaaat atccaaaacc aagtgatgag gaattgaaaa agaccctgtc
    25261 acctgaggag tatgcagtta cccaggaaaa tcaaacagaa cgagctttct caaaccgtta
    25321 ctgggataaa tttgaatccg gtatctatgt ggatatagca actggggaac ctctcttttc
    25381 atcaaaagac aaatttgagt ctggttgtgg ctggcctagt tttacccaac ccatcagtcc
    25441 agatgttgtc acctacaagg aagataagtc ctacaatatg acgcgtatgg aagtgcggag
    25501 ccgagtagga gattctcacc ttgggcatgt ctttacggat ggtccacagg acaagggcgg
    25561 cttacgttac tgtatcaata gcctctctat ccgctttatt cccaaagacc aaatggaaga
    25621 aaaaggctac gcttatttac tagattatgt tgattaatac tcttcgaaaa tctcttcaaa
    25681 ccacgtcagc gtcgccttac cgtatgtagg gttactgact tcgtcagttt tatctacaac
    25741 ctcaaagcgg tactttgaga aacctgtggc tatcttccta gtttactttt tgatttttat
    25801 tgagtataag aggtctttcc taagcagtta gaggagaatt ttgctatact gatactagta
    25861 agtggtaaag gagcagatca tgacctacac aatcttaatc gtagaagatg aatatctggt
    25921 aagacaaggt ttgactaaac tggtcaatgt agcagcctac gatatggaaa tcattggtca
    25981 ggctgaaaat ggaaggcagg cttgggaatt gatccaaaag caggtgccag atatcatttt
    26041 aaccgatatc aacatgcctc atctaaatgg catccagttg gccagtctgg tacgagaaac
    26101 ctatcctcag gttcatttgg tctttttaac aggttacgat gattttgatt atgccttgtc
    26161 tgctgtcaaa ctaggtgtgg acgactacct gctcaaaccc ttttctcgtc aggatattga
    26221 ggaaatgttg gggaaaatca aacaaaaact agacaaggaa gagaaagaag agcagttaca
    26281 agatttattg actaataggt ttgaaggaaa catggcccag aaaatccagt ctcatctggc
    26341 tgatagccaa tttagtttaa agtctttagc cagtgactta ggttttagtc cgacctatct
    26401 gagttccttg attaagaaag agttgggctt gccttttcag gattatctgg tgagagaacg
    26461 tgttaaacaa gccaagctct tgcttttaac tacagatctg aagatttatg agatcgcaga
    26521 gaaggttggt tttgaagata tgaactattt tacccaacgt tttaagcaga ttgcaggtgt
    26581 gacacctcgt cagtttaaga agggagaaga ccgatgaagc gttcttctct tttagttaga
    26641 atggttattt ccatctttct ggtctttctc attctcctag ctctggttgg aactttctac
    26701 tatcaatcaa gttcttcagc cattgaggcc accattgagg gcaacagcca aacgaccatc
    26761 agccagacta gccactttat tcagtcttat atcaaaaaac tagaaaccac ttcgaccggt
    26821 ttgacccagc agacggatgt tctggcctat gctgagaatc ccagtcaaga caaggtcgag
    26881 ggaatccgag atttgttttt gaccatcttg aagtcagata aggacttgaa aactgttgtg
    26941 ctggtgacca aatctggtca ggtcatttct acagatgaca gtgtgcagat gaaaacttcc
    27001 tctgatatga tggctgagga ttggtaccaa aaggccattc atcagggagc tatgcctgtt
    27061 ttgactccag ctcgtaaatc agatagtcag tgggtcattt ctgtcactca agaacttgtt
    27121 gatgcaaagg gagccaatct tggtgtgctt cgtttggata tttcttatga aactctggaa
    27181 gcctatctca atcaactcca gttggggcag cagggctttg ccttcattat caatgaaaac
    27241 catgaatttg tctaccatcc tcaacacaca gtttatagtt cgtctagcaa aatggaggct
    27301 atgaaaccct acatcgatac aggtcagggt tatactcctg gtcacaaatc ctacgtcagt
    27361 caagagaaga ttgcaggaac tgattggacg gtacttggcg tgtcatcatt ggaaaagtta
    27421 gaccaggttc ggagtcagct cttgtggacc ttgcttgggg ccagtgtcac atctcttctt
    27481 gtctgtctct gcttagtgtg gttcagtctt aaacgctgga ttgctccttt gaaggatttg
    27541 agagaaacca tgttggaaat tgcttctggt gctcaaaatc ttcgtgccaa ggaagttggt
    27601 gcctatgaac tgagagaagt aactcgccaa tttaatgcca tgttggatca gattgatcag
    27661 ttgatggtag ctattcgtag ccaggaagaa acgacccgtc agtaccaact tcaagccctt
    27721 tcgagccaga ttaatccaca tttcctctat aacactttgg acaccatcat ctggatggct
    27781 gaatttcatg atagtcagcg agtggtgcag gtgaccaagt ccttggcaac ctatttccgc
    27841 ttggcgctca atcaaggcaa ggacttgatt tgtctctctg acgaaatcaa tcatgtccgc
    27901 cagtatctct ttatccagaa acaacgctat ggagataagc tggaatacga aattaatgaa
    27961 aatgttacct ttgataattt agtcttaccc aagctggtcc tacaacccct tgtagaaaat
    28021 gctctttacc atggcattaa ggaaaaggaa ggtcagggcc atattaaact ttctgtccag
    28081 aaacaggatt cgggattggt catccgtatt gaggatgatg gcgttggctt ccaagatgct
    28141 ggtgatagta gtcaaagtca actcaaacgt gggggagttg gtcttcaaaa tgtcgatcaa
    28201 cggctcaaac ttcattttgg agccaattac cagatgaaga ttgattctag accccaaaaa
    28261 gggacgaaag ttgaaatata tataaataga atagaaacta gctaactccc agtcaattct
    28321 gggagtttta tgcgtaattg ggcaaacttt ctaggttgtc tatcttgcta aaaacaagaa
    28381 aaaacggtat gatagaagag tgacaacaga ggtaacagag atgaacgaga cacagattca
    28441 aagagaaaca cgtcaggtag tggaagatgt tttagaaaag accaatttga agcagggggc
    28501 cctttttgtt ttaggtctat cttctagtga ggtgctaggt ggtcagattg ggaaagaatc
    28561 cagtcaagag attggtgagc tgatagtgga gactatcttg ggtattctaa gtagtagagg
    28621 tatccattta gcagttcaag gttgtgagca tgtcaatcgg gctctcgttg ttgaacgtca
    28681 ggtggcagag cagtttggtc tggaaattgt cagtgtccat cctactcttc atgcaggagg
    28741 ttcggggcag ttggcagcct ttaagtttat gcaggatcca gttgaggttg aatttatcaa
    28801 ggctcatgct ggattggata tcggagacac tgcaattggc atgcatgtca agcatgttca
    28861 ggttccgatt cgccctattt tgagagagat tggtcatgcc catgtaacgg cactggctag
    28921 tcgtccaaaa ttaatcggag gtgcgcgtgc gcactatccg caagacgcta ttagaaagtc
    28981 ttgagaatga ataaaggaga taaaagtgtt taaaaaagac cgtttttcaa ttcgtaagat
    29041 taagggagtt gtaggctctg tatttcttgg aagccttttg atggctcctt ctgtagtgga
    29101 cgcagccacc tatcactatg taaataaaga gattatttca caagaagcta aagatttaat
    29161 tcagacagga aagcctgaca ggaatgaagt tgtatatggt ttggtgtatc aaaaagatca
    29221 gttgcctcaa acagggacag aagcatctgt tttgacagct tttggtttgc tgactgttgg
    29281 gagcttgctt ttaatctaca agagaaagaa aattgctagc gtctttctag ttggagctat
    29341 gggattggta gttcttccta gtgcaggggc tgtagaccca gttgcgaccc tagcgctggc
    29401 tagtcgagag ggtgttgttg aaatggaggg ctatcgctat gttggttatc tatcaggtga
    29461 catcctcaaa acgcttggct tggacactgt tttagaagaa acctcagcaa aacctggaga
    29521 ggtgactgta gtcgaagttg agactcctca atcaacaaca aatcaggagc aagctaggac
    29581 agaaaaccaa gtagtagaga cagaggaagc acctaaaaca gaagaaagtc caaaggaaga
    29641 accaaaatcg gagataaaac ctactgacga cacccttcct aaagtagaag aggggaaaga
    29701 agattcagca gaaccagctc cagttgaaga agtaggtgga gaagttgagt caaaaccaga
    29761 ggaaaaagta gcagttaagc cagaaagtca accatcagac aaaccagctg aggaatcaaa
    29821 agttgaacca ccagtagaac aagcaaaagg cccagaacaa cccgtgcaac ctacacaagc
    29881 tgagcaacca aggataccaa aagattcatc acaaccagaa gatcctaaag aagatagggg
    29941 agcggaagag acaccgaaac aagaagatac accgaaacaa gaagatacac agccagaagt
    30001 agtagaaaca aaagatgagg ctgctaatca acctgttgaa gaaccaaaag ttgaaacgcc
    30061 tgctgtagaa aaacaaacgg aaccaaaagt tgaacaagta ggtgaaccag tcgagccaag
    30121 tgaagacgaa aaggcaccag tctctccaga aaagcaacca gaagctcctg aagagaaggc
    30181 tgtagaggaa acaccgaaac cagaagataa aataaagggt attggtacta aagaaccagt
    30241 tgataaaagt gagttaaata atcaaattga taaagctagt tcagtttctc ctactgatta
    30301 ttctacagca agttacaatg atcttggacc tgttttagaa actgcaaaag gtgtctatgc
    30361 ttcagagcct gtaaaacagc ctgaggtaaa tagcgagaca aataaactta aaacggctat
    30421 tgacgctcta aacgttgata aaagtgaatt acaggaacaa ttaagagttg ctgaacaaaa
    30481 acaacaagct gactatagcg ctaaaacttg gagggaattc aaaattgctg aacttcaagc
    30541 taaagaaatc aacaaccaaa ctacaccact tcctaaacaa agtgaaatag atgcagctac
    30601 taaggctctt caagacgctc ttcaagctct tgctgtagat aaaactgtat tacaaaacgc
    30661 tattaacaca gctaatagca aacgtgaaga agagtatact gctcagacat ggaaagcgtt
    30721 agaagatgct cttacagcag tgaatcctgt taatgaagat gaaactgcaa ctcaaagcaa
    30781 agtagacgaa gctactagaa acttagaaga agctatcaat aacctagttc tattaactga
    30841 aaaaccggta ttaacattta ttgagactga taagaaagca ttagaacgtg aagtagttgc
    30901 taagtattct ctagaaaacc aaaataaaac aaaaattaaa tcaatcacag ctactctgaa
    30961 aaaaggagaa acagttgtta gcactgtaga gcttataggc gatgatgtaa ctaatgaaac
    31021 tataacctct gcatttaaga atctagagta ctacaaagaa tacaccctat ctacaactat
    31081 ggtttacgat agaggtgacg gtgatgtaac agaaatctta gataatcaac caatccaact
    31141 agatcttaaa aaagttgagc ttaaaaatat taaacgtaca gatttaatca aatacgaaaa
    31201 tggaaaagaa actaatgaat cactaataac aactgttcct gatgataagc ggaattatta
    31261 tttaaaaata acttcaaaga atcaaaaaac tacattacta gctgttaaaa atatagaaga
    31321 aactacggtt aacggaacac ctgtatataa agttacagca atcgcagaca atttagtctc
    31381 tagaactgct gataataaat ttgaagaaga atacgttcac tatattgaaa aacctaaagt
    31441 ccacgaagat aatgtatatt ataacttcaa agaattagtg gaggctattc aaaacgatcc
    31501 ttcaaaagaa tatcgtctgg gacaatcaat gagcgctaga aatgttgttc ctaatggaaa
    31561 atcatatatc actaaagaat ttacaggaaa acttttaagt tctgaaggaa aacaatttgc
    31621 tatcactgag ttggaacatc cattatttaa tgtgataaca aacgcaacga taaataatgt
    31681 gaattttgaa aatgtagaga tagaacgttc tggtcaagat aatattgcat cattagccaa
    31741 tactatgaaa ggttcttcag ttattacaaa tgtcaaaatt acaggcacac tttcaggtcg
    31801 taataatgtt gctggatttg taaataatat gaatgatgga actcgtattg aaaatgttgc
    31861 tttctttggc aaactacact ctacaagtgg aaatggctct catacagggg gaattgcagg
    31921 tacaaactat agaggaattg ttagaaaagc atatgttgat gctactatta caggaaacaa
    31981 aacacgcgcc agcttgttag ttcctaaagt agattatgga ttaactctag accatcttat
    32041 tggtacaaaa gctctcctaa ctgagtcggt tgtaaaaggt aaaatagatg tttcaaatcc
    32101 agtagaagtt ggagcaatag caagtaagac ttggcctgta ggtacggtaa gtaattctgt
    32161 cagctatgct aagattatcc gtggagagga gttattcggc tctaacgacg ttgatgattc
    32221 tgattatgct agtgctcata taaaagattt atatgcggta gagggatatt cgtcaggtaa
    32281 tagatcattt aggaaatcta aaacatttac taaattaact aaagaacaag ctgatgctaa
    32341 agttactact ttcaatatta ctgctgataa attagaaagt gatctatctc ctcttgcaaa
    32401 acttaatgaa gaaaaagcct attctagtat tcaaga
//
LOCUS       DAVMAF010000018        27041 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMAF010000018 DAVMAF010000000
VERSION     DAVMAF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27041
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.018"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..415)
                     /locus_tag="VYO97_000817"
     CDS             complement(<1..415)
                     /locus_tag="VYO97_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1753641.1"
                     /translation="MFMKLLHFSEVGGGVDRYIKLYLKYSDKEHFKNIVVGSDQLNRQ
                     TYEQEYNIKFYHIDIYRSLSPIKLLRAIKQFRKILYLERPDIVYLHSTFAGVVGRLAS
                     MGLSCKVVYNPHGWSFKMDVSKIKQFVYKNIEKFLS"
     gene            complement(422..1789)
                     /locus_tag="VYO97_000818"
     CDS             complement(422..1789)
                     /locus_tag="VYO97_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1753642.1"
                     /translation="MNGKIVKSSLVIIQSSLVILLTYLLSTVREAKIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDGLIESNEVVGEL
                     VAVSVLDKPDFQHDYLKIVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIRKMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGCFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            complement(1804..2493)
                     /locus_tag="VYO97_000819"
     CDS             complement(1804..2493)
                     /locus_tag="VYO97_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1753643.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     LGEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSIDKYGSY
                     GNYGNYGKNKK"
     gene            complement(2503..3195)
                     /locus_tag="VYO97_000820"
     CDS             complement(2503..3195)
                     /locus_tag="VYO97_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEV1753644.1"
                     /translation="MKEQNTIEIDVFQLFKTLWQRKLMILLVALVTSAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVAVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKMK"
     gene            complement(3204..3935)
                     /gene="cps4B"
                     /locus_tag="VYO97_000821"
     CDS             complement(3204..3935)
                     /gene="cps4B"
                     /locus_tag="VYO97_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1753645.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(3937..5382)
                     /gene="cps4A"
                     /locus_tag="VYO97_000822"
     CDS             complement(3937..5382)
                     /gene="cps4A"
                     /locus_tag="VYO97_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1753646.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSTLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIKKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            5605..6126
                     /locus_tag="VYO97_000823"
                     /pseudo
     CDS             5605..6126
                     /locus_tag="VYO97_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6119..6286)
                     /locus_tag="VYO97_000824"
     CDS             complement(6119..6286)
                     /locus_tag="VYO97_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1753647.1"
                     /translation="MKKRILSAGLTFTSALLLAACGQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(6451..8058)
                     /locus_tag="VYO97_000825"
     CDS             complement(6451..8058)
                     /locus_tag="VYO97_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1753648.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENKKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKRVLAPWDAFCVELL"
     gene            8211..9695
                     /locus_tag="VYO97_000826"
     CDS             8211..9695
                     /locus_tag="VYO97_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1753649.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            9790..10272
                     /locus_tag="VYO97_000827"
     CDS             9790..10272
                     /locus_tag="VYO97_000827"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1753650.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(10339..10674)
                     /locus_tag="VYO97_000828"
                     /pseudo
     CDS             complement(10339..10674)
                     /locus_tag="VYO97_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(11126..13231)
                     /locus_tag="VYO97_000829"
     CDS             complement(11126..13231)
                     /locus_tag="VYO97_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1753651.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMSQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISDL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(13840..14820)
                     /locus_tag="VYO97_000830"
     CDS             complement(13840..14820)
                     /locus_tag="VYO97_000830"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1753652.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(14822..17074)
                     /gene="pbpX"
                     /locus_tag="VYO97_000831"
     CDS             complement(14822..17074)
                     /gene="pbpX"
                     /locus_tag="VYO97_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1753653.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(17078..17395)
                     /gene="ftsL"
                     /locus_tag="VYO97_000832"
     CDS             complement(17078..17395)
                     /gene="ftsL"
                     /locus_tag="VYO97_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1753654.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(17407..18357)
                     /gene="rsmH"
                     /locus_tag="VYO97_000833"
     CDS             complement(17407..18357)
                     /gene="rsmH"
                     /locus_tag="VYO97_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1753655.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            18523..18717
                     /locus_tag="VYO97_000834"
     CDS             18523..18717
                     /locus_tag="VYO97_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753656.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            18733..19275
                     /locus_tag="VYO97_000835"
     CDS             18733..19275
                     /locus_tag="VYO97_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1753657.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            19286..19528
                     /locus_tag="VYO97_000836"
     CDS             19286..19528
                     /locus_tag="VYO97_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753658.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            19539..19874
                     /locus_tag="VYO97_000837"
     CDS             19539..19874
                     /locus_tag="VYO97_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753659.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(19984..20985)
                     /locus_tag="VYO97_000838"
     CDS             complement(19984..20985)
                     /locus_tag="VYO97_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1753660.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(21045..22946)
                     /locus_tag="VYO97_000839"
     CDS             complement(21045..22946)
                     /locus_tag="VYO97_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1753661.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(22968..23261)
                     /locus_tag="VYO97_000840"
     CDS             complement(22968..23261)
                     /locus_tag="VYO97_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1753662.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(23261..24079)
                     /locus_tag="VYO97_000841"
     CDS             complement(23261..24079)
                     /locus_tag="VYO97_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1753663.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(24066..24845)
                     /locus_tag="VYO97_000842"
     CDS             complement(24066..24845)
                     /locus_tag="VYO97_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1753664.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(24860..25351)
                     /locus_tag="VYO97_000843"
     CDS             complement(24860..25351)
                     /locus_tag="VYO97_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1753665.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(25362..26552)
                     /locus_tag="VYO97_000844"
     CDS             complement(25362..26552)
                     /locus_tag="VYO97_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1753666.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(26564..26998)
                     /locus_tag="VYO97_000845"
     CDS             complement(26564..26998)
                     /locus_tag="VYO97_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753667.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         7957 a         6106 c         4764 g         8214 t
ORIGIN      
        1 aagacaaaaa cttttcaata tttttataaa cgaattgctt aatcttagaa acatccatct
       61 taaaagacca tccgtgagga ttgtatacta ctttacacga caaacccata gaagctaacc
      121 tgcctactac acctgcaaaa gtactgtgca gatatactat atcaggtctt tctagataca
      181 atatttttct aaattgttta atcgcgcgta aaagctttat tggagacaag cttctataga
      241 tatcaatgtg ataaaacttt atattatatt cttgttcata tgtttgtcta ttaagctgat
      301 ctgatcctac cacaatattt ttaaaatgtt ctttatctga atattttaaa tataacttaa
      361 tatacctatc aactccaccg ccaacttcac taaaatgaag caatttcatg aacatatccc
      421 cctatttggc tccatccttc atcaatacta ccttaactgt cttcaataaa atttcaatat
      481 ctttccagat tgtccaatca tctatataag ccacatctaa tttgacaact tcatcgaaat
      541 ttttgatttc acttcgtcca ctgacctgcc ataagcctgt tataccaggt ttaaaactta
      601 gccgacgttt ttgttctggg gtatagtgtt catactcatc cactgttggt ggccgtgtgc
      661 caaccaaact catatctccc tttagaacat tataaaactg tggtagctcg tccaagctag
      721 tcttccgtat aaaacaacca attttcgtga tacgaggatc atcgtccacc ttaaacattc
      781 caccctgcat ggtattttgt tccatgagtt ctcttttttt cgcctcggca tctacacaca
      841 tagagcgaaa cttataaaaa gtgaactgac gaccattttt tcctatacgc gtctgagcaa
      901 aaatagcaga gcccccatcc tttcgaatca aaggaaccag tacaatactg actaaaccac
      961 ataatatcaa cccgaccaat gcacccacga tatcaataat ccgcttagca attacatgac
     1021 tagtcttata aaatgttgta gaaaaagtca caacgtttaa tcctgccatc ttacgaattt
     1081 gcttgttacg tgccaaacta cgatcaaaag catttagatt gactgttaca tcaattccca
     1141 tcgtttcaaa ctgagagaca agctctccaa tattgtattt ttcacttgga agattgataa
     1201 agacttcatc gaccacctca tgagtcgcaa agtttactat ctccccctct gctactatct
     1261 ttaaataatc atgctgaaaa tctggtttat ctaagacact gacggctacc aactccccaa
     1321 caacctcatt tgattctatt aatccatcca gtaccttttc gacacgagaa gttgctgtaa
     1381 gtaggagaat cttcttactt cctttaaagt tgggataagc ccgcttccaa taccacttga
     1441 taaataggtt tagcacatag actaagagag catgtaatgt gaggaagtaa atcatgcctc
     1501 gtctggaaat actaaatcga tcctctaaga aaaaattaga aatactaatc gctagtgcaa
     1561 agaataggat atatttcaat gtctggacaa gttcaatcaa atatcccctt ttaaagaaat
     1621 cctgtccata atcactgata taaaagacaa aataatggag gatataaagt gcaatagctg
     1681 ttgttgaaac aatcttcgct tctctcacag tactaagtag ataagtcaat aaaataacaa
     1741 gagaactctg gattatgacc aatgaagact ttactatttt tccattcatc tctatccccc
     1801 gacctatttt ttatttttcc cgtaatttcc ataatttcca taagaaccgt atttgtctat
     1861 tgaagtatcg aatttattca acacaactcc caaaaacggc ttccctgtgt gttccaactg
     1921 ttcttttgct ttttgaatat cccgtcgatt tatttcacct gcctccgtca ctaaaataga
     1981 agcatcgcat tttcgcgtaa taatagccgc atcaatcacg acaccgacag gagcagtatc
     2041 tacaatgatg tagtcaaaat atttacgcaa ggtttcaagc attgtactga aattcttact
     2101 ttgaagaaga gctgtcggat tcggtgacac agagccagcc tgaattacaa agagattttc
     2161 gatattggta tcacaaagcc cttgtgatag gtctgtagtt cctgataaaa attctgtcag
     2221 gcctgtaatc ttatcccttg ctttaaagac acctaacata acagaattgc gaatatctcc
     2281 atcaatcagc agcgttttgt atcctgcacg cgcaaaagcc caagcgatat tggtggaagt
     2341 cgttgatttt ccttctccta gtttcacaga agtgatagaa aatactttca aaccatctcc
     2401 acttaactgt aggttcgtgc acaaagcgtt ataatattcc tctgcctttt ttacagaatc
     2461 caattttgcc tgtgagattt ctaatgttgg cataacttcc tcctatttca ttttgtcaaa
     2521 atctggaacg agccctaaaa gtggaatttt cagtacatct tcaacatctt caggacgttt
     2581 cacacgggtg tcgagcaact caatcaaaag aacagcaatt actgttacga ctgctcctcc
     2641 aagaaaacca aataaggaat tgcgtcgaac atttggagaa gagggagtcg tagctggtcg
     2701 cgcttcttca agtgtcgtta catcagatac tcgcgttaca gcgacgatct tttctgcagc
     2761 aacttctcgt agagaattag cgatacgact ggcttcctct ggctgtttat ccttgacaga
     2821 gattgagacg atacgagtgt cagctggtac agccacttga actttgctgg ctaacgtttt
     2881 tgctggcata tccaacttca aatttgtcgc tactttttcc aatgcatcct gcgaaaggat
     2941 aatctcacgg tagtctttca ccagataagt tcctgcctgc aaatcctgat ttgtcagccc
     3001 cggcttgtct ccttgattgc gattcactac gtaaattcgc gtggtactcg tatattctgg
     3061 cttaacaata aaagtgctat atgcaaaagc ccccgcactt gtcacaagtg ccactaataa
     3121 aatcattagc ttgcgttgcc acaaggtttt aaataattga aatacatcga tttctatcgt
     3181 gttttgttct ttcatcattt ctcctaaatt agttgatcca ttacaatttt tcgaggattg
     3241 tctataaaaa gttcctgagc cttcgcttct ccgtattttt gggtaacaag gtcatatgct
     3301 tctgccatat gaggaggtct accgtctaga ttgtgcatat cacttgcaat gacatggacc
     3361 aaatcctgct ctaaaaaata ctgagctctt tttttcatga atttataacg ttcgccaaaa
     3421 agtttgggtt tgaggacatg tgaactattt acttgcgtgt aacagcccat atcgattagt
     3481 tctcgaacgc gtttttcatt attttcaaga gcatcatagc gctcaatgtg ggcaatgact
     3541 ggagtaattc ccaacatcaa gatcttgctc aaggcgctat gaatatcgcg ataaggagtg
     3601 ttcatactaa actctatcaa ggcataacga ctatcattga gggtcggaat ccgctttttt
     3661 cccagcttat ccagaacatc tggtgtgtag taaatttcag ccccgtaagc aatgactaag
     3721 tcactcgcca cttccttagc tatttcccga acctgaagaa agttttctgc tatcttctct
     3781 tccggagttt caaacatgcc cttgcgacgg tgagaggtag agacaatggt tcgcaccccc
     3841 tgtctgtagg cttctgccaa gagagccttg ctttcctctc ttgacttggg accgtcatct
     3901 acatcaaaaa cgatatgcga atggatgtct atcatttcat ctaccctcca tcacatcctg
     3961 tatagctgct ttaactacag ctaaactact atcatctatt tccatcacat agaggttact
     4021 gtctggcatt gcataagaag gaagatccat ccgacctgta cctttcaagt cttgcgaatt
     4081 tactttataa ttccccccac tttccaactg agtattcact aaatctatca tagtctcaat
     4141 cggcatattt gtttgaagag aatcctgcaa tccttgaaga atactactat agtttttcaa
     4201 aacctctgta gaagttaact tcttaataat tgctacaatg accttttgtt ggttgcgacc
     4261 acggtcacgg tctccatcgg ctagtgagta gcgttcacgt acaaaaccta gagcctgctc
     4321 agagtctaga tggacattcc ctactgggaa atggaacttc ccatgtagag ttgaaaactc
     4381 ttgatcatta tgaacatcta ctcctcccaa taagtcaatc attttcaaga aagaagtgaa
     4441 gttcaatcgc acatagtaat tgatatccac tccatagaga ttttctaagg tgtgaatgga
     4501 cgaatcaact ccataaatgc ccgcatgggt taatttatct ttttgattat ttccaccatc
     4561 tgcgattggt acataggcat cacgtggcgt tgtggtcaag aggattttct tggtatctcg
     4621 attgacagtc atcaggatat tgacatctga tcgcgacacc gaactaatag gaccataggt
     4681 gtcaattcca ctaacataga tattgaaaga ctgattctta gacgtcttag gagcttctac
     4741 ttttttagtg aatcccttgg tataaatctt ttttatcttc gatgcatagt ctggatactc
     4801 tgactcgatg atattttcaa agacactatt taagacaatg gccttagtct cccctgcaat
     4861 caaactcttg taagctgcca agtaagacga actctgatcg accgtcaaat cggtattctg
     4921 acttgacttg atatcagcta gtagtttctg aatattttca ttatcagtcc cagtcggtgc
     4981 tgtcacactc gtcagttgcg taacattttc gatctcacta tctgctaaaa cagcgacact
     5041 gattgaatat tctgagtaat tagaagtcgc atttaaacga ttggtcagtc caacaaactg
     5101 ctgtactgca aagagcgaca cagagctgac aaggatagag aacaccaaca gaaaaatagt
     5161 aaatttttca gcttttttat agataatcag gagtagccct accaaggcaa ctagtaggac
     5221 taacgcagtt accactagat taagatatct aaaagcaagg atattgtact taaagattaa
     5281 gaacaataaa aaacaaacta acaataaata aatagtcaac aaaactatat taacacttcg
     5341 cttcactttc tgtgaacctg attttttaaa acgtctactc atgattaaca cctatacatt
     5401 gaacatcata cgattatatc acttttttac ggtaatgtct acaccttttt tcactatctg
     5461 catctttaag tatcttagta gacttcccgc gaaacaaaaa tatagtaaaa tgaaataaga
     5521 acatgacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag aagcagaggt
     5581 gtactattct agtttcaatc taattatgaa caagacccag aaaaagtagc cttatttctt
     5641 aagaatttta atagtttaaa gcacctagca cctgtttaga ttgacgaaac aggattcgat
     5701 acttattttt atcgagaata tggtcgctca ttaaaaggtc agttaagaag aggcaaagta
     5761 tctggaagaa gatatcagag gatttctttg gttgcaggtc taacaaatgg tgagttaatc
     5821 gctccaatga cttacgaaga gacgatgacg agcgactttt ttgaagcttg gtttcaaaaa
     5881 tttctcttac caacattaaa cacatcatcg gttattatta tggataatgt aagattccat
     5941 agactgggga agctagaact tttatgcgaa gagtttgggc ataaactttt accttcttcc
     6001 tccctactca cctgagtaca atcctattga gaaaacatgg gctcacatca aaaagcctcg
     6061 taaaaggtat taccaagttg caataccttt tatgaggctc ttttgtcttg ttcttgtttc
     6121 aattgactat attatcagcg taatagtcta atagatagtt aaatgtagtt ggatttccac
     6181 taaaggttga tgagtaagtt tttgtatctg aacctgattg gccgcaagca gctaaaagca
     6241 aagcagatgt aaaagtcagg cctgcactaa ggatacgctt ctttatgttc gtcttctttc
     6301 tccttaatag tgggaatttg taaagttaat tgaatttcga gaatgaaggt ttataaacct
     6361 tggttataaa aaacaaagga tttctgcctt ttatacagtc ctcccttgtt tttatacgat
     6421 ttcaatttta aatttttctg caaaaaatat ttatagtaat tccacacaga aagcatccca
     6481 tggagccaag acccgttttt caagtacttc tttagccgca gtgttttcaa tcaagacaga
     6541 tttgactttt ccttctactg tcaagtcttg ctcttcattg gacaagttag ccacaactag
     6601 gaagcgacgg tcgccatcct tacgtatata agcaaagacc ttatcagccg tatcaagcaa
     6661 ttcaaagtca gctcgaatta gccagctatt ctccttgcga atttggacca gtttctgata
     6721 ggtatagaaa atagaatctg gatttgccag cgcttcttgg acattgatca tctcgtaatt
     6781 tggattaacc gccaaccaag gttgacctgt tgagaaacca gcgtttttgc tctcgtccca
     6841 ttgcataggg gtacgggcat tgtcacgtcc aataacacgg atactgtcca tgatttcttc
     6901 aatcggaaca cctttttcaa gagcctcacg cgcatagttg agagattcaa tatcttctac
     6961 ttgatccagt gtttcaaacg gatagttggt catcccaatc tcctcacctt ggtagatata
     7021 aggagttcct ctcataagat gaagcaagat tgcaaaggct ttggcagatt tttcgcggta
     7081 ttcttggtca tttccccaga ttgagacgat acgagggagg tcatgattgt tccagaagag
     7141 ggaattccaa ccgtcctcaa ctcctaactc tgtctgccat ttgttgaaga tttcttttaa
     7201 cttagcgata ttcagctctt tttgatagtg ccatttaggc tgaccttcct gatactgaag
     7261 accgatatgt tcaaactgga agaccataga caattcttgc ccctttggat cagagtagag
     7321 cttggcaatc tctggcgttg ctccccaagt ctcccctact gtcaagagat ccttagctcc
     7381 aaaggtcgcc tgattcattt ccttgagata gggatggagc ataggaccat tattgactac
     7441 cttctcatca ggaattttgc caatcatgtc aataacatcc atacggaaac caccaatacc
     7501 tttatcaatc cagaagttca tcatctcata aattttctgg cgaagttttt tattttccca
     7561 gttgagatcc ggctgtttct tgctgaaaaa gtggagatag tattgaccag atttttcatc
     7621 gtattcccaa gcagatccac taaaaataga atctaaatcg ttaggctcat cccgccagat
     7681 atagtaatct cgctcagggc tattaggatt ttcacaggcc tctacaaacc aggcatgctc
     7741 atccgaggta tgattgacca ccaagtccat gatgatacgg atatcacgct tcttagcttc
     7801 tgcaatcagt tgatccatgt cctccatggt tccaaaaata gccgcaatcg cttgataatc
     7861 agcaatatca tagccattat catccatagg gctgtcataa acgggagaaa gccaaattgc
     7921 tgtgattcct agcttggcta gatagtccaa cttactggta attcctggca aatcgccaat
     7981 tccatcttca ttactatcca taaaactctt tggatagact tgatagacta cggcattgtg
     8041 ccaccatttt tcttgcatct tcttacctca ttattttcat aatctatagt ctatgatagc
     8101 actttttaaa attttgtgca agcgtttgct taatcttttt aatttctttt ttagctccgt
     8161 agtttcctaa cttccaattt tttattataa tagagtccag aggtaaaaaa atgaaaaaac
     8221 aagcttttag ttctgaacaa tatttaaatc tacaacgcga ccacatctta gagcgcatta
     8281 accaatttga cggcaagctt tacctagagt ttggtggcaa aatgttagaa gatttccacg
     8341 ctgctcgtgt ccttcctggt tatgaacctg acaacaaaat caagctcttg caagaattga
     8401 aagagcaagt tgaggttgtg attgccatta atgctagcaa cattgaacat tccaaagcac
     8461 gtggcgactt gggcatttct tatgaccaag aagttcttcg tttgattgac aaattcaatg
     8521 aattagggat ttttgttggt tccgttgtca ttacacaata cgctggccaa cccgctgcag
     8581 atgccttccg caaccaactt gagaaaaacg gaattgattc ttatcttcat tatccaatca
     8641 aaggatatcc gacggatatg gatcacatca tttccccaga aggcatgggc aaaaacgact
     8701 acatcaaaac cagtcgcaac ttgattgtcg taaccgctcc tggacccggt tctggaaaat
     8761 tggcaacgtg tatgtccaat atgtaccacg accaaatcaa tggcatcaag tctggctacg
     8821 ctaaatttga aaccttccct gtttggaatc ttccccttca tcacccagtt aacttggctt
     8881 atgaagctgc cacagctgac cttgatgatg tcaacatgat tgaccccttc catcttcaaa
     8941 cctatggaga aaccactgtc aactacaacc gtgatatcga aatcttccca gtgctcaagc
     9001 gcatgttgga acgtattctc ggaaaatcac catacgcttc accgacagat atgggtgtca
     9061 acatggttgg tttcgctatt acagatgacg aggctgctgt cgaagcttct aaacaagaaa
     9121 tcatccgccg ttactatcaa acagttcttg acttcaaagc tgaaaaagtt ggcgaagctg
     9181 ccgtcaagaa aattgagttg ctcatgaacg acctcggtat cacacctgca gaccgtaagg
     9241 ttgctgtcgt tgcgcgccaa aaagcagaag aaactggtgg accagcccta gcctttgaat
     9301 tgccaaatgg ggaaatcatc actggtaaga actcagaact ctttggccct acagccgctg
     9361 ccttgatcaa tgccatcaaa aaatcagctg acatcgctaa agaagtaaaa ctaatcgagc
     9421 ctgaagttgt taagccaatc caaggtctta aaatcgatca tctcggtagc cgcaatccac
     9481 gccttcattc aaatgaaatc ctgattgcac ttgctatcac agctacagaa aatcctgatg
     9541 ctgcccgcgc tatggaagaa ctcggcaacc tcaaaggaag cgaagcccac tcaaccatca
     9601 tcttgactga tgaagacaag aatgtccttc gtaaactggg tatcaacgta acctttgacc
     9661 catactacca atacgaccgc ttatatcgta agtaaagatt tcaacctctc tagcctcgga
     9721 gaggttttct ctctgtctca ggaacaacct ttatgatata atgagagaag aaaactgaaa
     9781 ggatttacca tgtcaaaaga agttattgtc gaaagttttg aacttgacca caccattgtc
     9841 aaagcaccct atgttcgctt gattggggaa gaaacaggac caaaaggaga cattatctcc
     9901 aattatgata ttcgcttggt gcaaccaaac gaagactcta tccctactgc cggccttcac
     9961 actatcgagc acctcttagc caaactcatc cgtacccgca ttgacggcat gattgactgt
    10021 tcaccatttg gttgccgcac aggcttccac atgattatgt ggggacgtca caccagtgct
    10081 aaaatcgcag ctgttatcaa ggattcgctc aaggaaatcg ctgaaactac tacttgggaa
    10141 gatgtcccag ggacaaccat cgaatcttgc ggaaactaca aggaccacag cctcttctct
    10201 gctaaagaat gggcaaaact catcttggaa caagggattt cagatgatgc ctttgaacgt
    10261 catgtgattt aaaatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtgc
    10321 cgttcggcat acggcggttc aactaacttt taacgcatgt cgttcaaggt aataatccaa
    10381 acacgaaacc agtccacgtt tttcaaggac tggttttgat atagcacgtt taagtaccga
    10441 cttctgagct actaattgat agtggtcgcc ccagccagat accttatctg ctatccattt
    10501 aggaacccct aacttaagca atccccataa tcgtctcgat tttttcttcc attgcttcca
    10561 gataatcact cgtaggcgag tatgcaagcg ctcatctatg ctagtgacta tacttttcat
    10621 atttcccaat gagaaatagt ttatccatcc tcgaacagac aaattcagtt gctctcgtga
    10681 tttacgcata taggctacga ctaccttttt gaactttaga gtttgaggcc ccttatccgc
    10741 tatatacgcc ttattatcac gttcctattc gtagagccac gatttcgcta tcccttcctc
    10801 tgacgattct tatttccagc gtcagactta aaacggtcta tccccagacc gttttaatcc
    10861 gctacctcac gatagtcagg cttgggaatc actattggga gcaccggtag caggtaccca
    10921 ccgaggactt acacctcaga tatatagtaa aatgaaataa gaacaggaca aatcaatcag
    10981 gacagtcaaa tcgatttcta acaatatttt agaagtagat gtgtactatt ctagtttcaa
    11041 cctactgtac gacatgcccg tcgtacctaa aaaggaacca gtttttttca gctggctcct
    11101 tcttttttat cctcaaaatt ctgtcttagg ctttctcacg aataaccaaa acgccatctt
    11161 ccatatctgc ttccagatgt ttagcatcta aatgatccaa gtggaagtct gtcaccttat
    11221 cacgaatttc ttgttcaacc actcgacgga gaggacgaac ccccatgact tcgtcgtaac
    11281 cttcttctgt gatataatct ttagccgctt gactaactac caagtcaatg tctttcttag
    11341 ccaaggtttg gttaacttca gccaacatca aatctacaat cttagaaagg tcttccttag
    11401 tcaagtgtga gaactcgatg actgcattaa agcggttgag gaattctgga cggaagaagg
    11461 gtttcaaacg gtccatcaat tctggtttat ccgcatcttc tgtcaagttg gcttcatagc
    11521 caaatccagc atttgaggtc gcaataatga cagtgttctt gaagtttact gtatttcctt
    11581 gaccatctgt caaacgacca tcatctagaa cttggaggag aagggtaata acttgagggt
    11641 cagccttttc aatttcatcc aagagaatga tagagtatgg attgcgacga acacgttctg
    11701 ttaaggtatt gctattgtca tcataaccca catagcctgc tgttgtacca attagcttag
    11761 aaacagctgt acggtcactg tattcagaca tatccaaacg gataatcgca tcctgggttc
    11821 caaacatatc gagtgccaat tgcttagcaa gctccgtctt accaacccca gtagacccta
    11881 caaagaggaa gctgccgatt gggcgatttc cttcatcaaa accagcacgg ttacgacgga
    11941 tagcacgagc tacagcttct acggccttat cttgaccaat caccttgtct tgcaagcgat
    12001 gagccatatc tttcaaacgt tcgatatctg aagctcccat ttgcgatact gggatacctg
    12061 tcattcgttc cacagattca gccacatcgt tgacacttgc agtcactttc atatcttctg
    12121 tgtggttttc gattttcctt tccaattctg caatgcgtgt tttatagttt agagctgctt
    12181 caaaatcttc tgcttcaact gctttttctt gcttgtcttt ttccgtttcg atttctcgtt
    12241 caacagcatg cacatctgtt actggatgtt gagccgccaa gtgagcagcc gttacatcga
    12301 caaggtcaat agccttatct ggcaagctac gttgaggaat gtattgaaca gaataatcca
    12361 ccgctgcttt caagacttcg tctggcaaga tgacattgtg gtgttgttga tagagatcac
    12421 gaatgccttg aagaatttta aaagtattct ctgctgaagg agcattgacc ttcacttcgt
    12481 tgaaacgacg agcaagagca gcattcttca agatggtgtt acggtattcg tcttgagttg
    12541 ttgccccaat cactgtcaat tctccacgag agagagctgg cttgagaata tccgcaagtc
    12601 ctttagaacc actgtctcca ccagtgctac cagcaccaag aatttggtga atttcatcaa
    12661 agaagaggat aatattccct gcttctttca cttcattgac taagttttgg acattttctt
    12721 caaagctacc acggtattga gtaccagcct caagatctga gatatcaata gaaataattt
    12781 ccttgttctt gatagcagca ggaacatctc cgttcacaat ggcttgcgct agaccttcga
    12841 caactgctgt cttaccaaca cctgcatctc cgaccaaaac aggattgttc ttggtgcggc
    12901 gtgagaggat ttcagatgtt tcttgaattt ccttgtttcg tccgataaca ggatccaact
    12961 tgccctcacg cgcttctgct gtcaagtttc gacctagttt tgcaaggaca ccgtcttgtt
    13021 tcatacctga tgcctgttgt gacatttgca catcagtttc tgcatttcct ggtaattgac
    13081 cagtcgtacg atagtgagca aattcctcag gtgtgacttc gcgcccatta attaagtaac
    13141 ggcgattttc agaactgtat cctcgcatac cacccatcaa ttggttaaat aaatcatcca
    13201 tgttattaaa attattaaag ttgttattca tattctttac ctctttttgt ttacttagtt
    13261 atgattactg atattgacta tctttgacct ttgttttaaa aaatttagac tagctaaacc
    13321 gctaccctac gtactatttc tggtttttct ttttcaagca ggagtagact atctttactt
    13381 ctgaaaattt ctagatgaaa catagaccca cctctttcta ttttacttta aataagtgtt
    13441 ctagtaaggc tttcatttac cttacgttca taatatacta cattggtcag aaaaggtcaa
    13501 gggttttgac tttaactgac caatattttt ttacactctt cgaaaacctc ttcaaaccac
    13561 atcagcttct ccttatctac aacctcaaaa ccgtgttttg agctgacttc gtcagttcta
    13621 tcaaccatct caaaactgta tattaaaacg aaataagaac aggacaaatc aatcaggaca
    13681 gtcaaatcga tttctaacaa tattttagaa gtagaggtgt actattctag tttcaaacta
    13741 ctatgttttg agcagcctaa agttagcttt ctagattgtt ctttgatttt cattgtgtat
    13801 ttaaacacaa aaacaccctg aaacatcagg gtgccattct tacatcaaat ataaaattgc
    13861 gagggtcagg agacttgcta gaagtcccac tccccaaaag aagaagtcaa ccttccactc
    13921 gctccaagga tttcctttac cagacaatcc cccaagctca aaatggtgat gtacaggcgt
    13981 catacggaaa atacgtttac cacctgtcag tttgaaataa ctgacttgca tcataacaga
    14041 agttgtttca aaaacataca caattccgat aatcaagaga gtccattctt ggtggagagc
    14101 catagagata gctgccagca tcccacctag ggccaaactt cccacatcac ccataaagac
    14161 cttggcaggc ttatggttaa agataaagaa accgagcaaa ccaccaatca tggcaagaat
    14221 cactagaaga atatccatct gaccttgcac ataggcaata actccatagg cagacaaact
    14281 aatcacaacg gaaatactag ctaaaccgtc aacaccgtct gtcaagttta ctgcgtttga
    14341 aaaaccgact agccagaaaa gagcgaagaa aatatagaaa aatcccaaat gaactggata
    14401 accaaagaca gacaggatat cgccaccgcg ctcatagaaa agatagaaga taactccacc
    14461 tagaagctga agagctaatt tttgcttagg attaagcccc tcattgattt tacgaaagac
    14521 cttgagaaag tcatctaaaa atccgaccaa gccatacaag accaagatga acaaaatcat
    14581 tcccacatta ttgctgaatt ggctactaaa tagggcgaaa aagaaagcaa ccaaaacaga
    14641 agtaatcaag aaaaccaaac ctcccattgt aggagtccca gcttttgcct gatgctgttt
    14701 gacatcctca tgcatctgct ggcctgtaat ttgcgccttt ctataaaatt ggataaaggc
    14761 cggaatttct actaaagtta gtaaaaatgt cacaattcca gcactgatgg aaataaacat
    14821 attagtctcc taaagttaat gtaatttttt taatgtcctt gatagctgtg ttagcacgaa
    14881 catcttgctt ctgcacagta gagcccgaac cttgaaattc aagttctata ttgagccact
    14941 tagcaagggt ctcagcagtc tcctttgtcc aaccatacat atctggaact tcctctgctt
    15001 tatcagataa gataaggacc tgctgattcg gggcaagatt cttcccttct tcagcagaac
    15061 tgtttttaat cttcgttcct gttcccacaa cgatgggttg tacaagattg cgacgcaatt
    15121 cttctgctaa atcaccaggt gaaatatcct tgacactagg cataggataa ggactttgtt
    15181 gacttacttg ctccaaagcc ttagctgttg tttgaagatt gagagagtct ttcatagctg
    15241 aagcccgctc caagatagga ttggcaaatt ctcccaactg aatacctgaa taatgttcag
    15301 gttgttggac cgtcacatac aagataaaat caggattttc agccggactc atcgatacag
    15361 ccgagaaaat atagtcggtt aacccgacta gataaccacc atttttctcg tcagcaatct
    15421 gagccgtacc agacttgagg gctacatttt gcccaggaac agttacagtt ggcttgcctg
    15481 tgctgtggtt atacatggtt ccataaaccg gatccgtccc taccaaaacc atgttagtcc
    15541 gagttagact agctgcatct ttagaaacag gatttcccac aatttctttt tgagatttcc
    15601 gagcagtttg atcatttgga tcataaatgg cactaataaa tttaggctcc agcatgacac
    15661 cgtcattagc aatagctgta aaggcacgaa tcatttgcgt ctgggtcact gaaatccctt
    15721 gtccaaatga gctttgcgca atgttgacaa tattatccgc aggaagctga ccagcatact
    15781 catccgtcaa accgaaacgg gtcggaacac caaatttaaa acgattaaga taatcaagcc
    15841 aggtagcatc tcccatcttt tgctcaagga gagtcatccc aacgttactt gagtgtgcaa
    15901 aaccttgaga aaaagtcatc attctgccac cagtcaatcc ttcattaacg tcccaatctc
    15961 gaatcgtggc atctgcaatt tttaactcac tactattaaa gacttctcct cctggaaagg
    16021 tattattatc aatagcagca gccaacatca tcactttcat agtggaacct ggctcatagt
    16081 tactttggta aaggatatca cgccaaacaa agtcctctgt aatgccttct tttgtatctg
    16141 catcaaaggt cggtcgttgc gttgttgcca gaatttcccc tgttttagca ctgaccaaag
    16201 tcgctgtcat gtactttcct tttaccttct cttgaaaagc atccatctgg gtttccataa
    16261 aggactggag ggggctggaa atggttgtat aaacatcctt accgtccatc gttcgttggg
    16321 aaacttgttc tgttccaggt acaatattac ccagacgatc cttttcatag gtaataatgc
    16381 cgtctgtccc tgcaagaata ctgttcaagg aactctccat tccagaggtt cccagcaagc
    16441 tcttgcttcc atcttcattt tcatggagct gagctaggcc gataaaacta gaagcaaatt
    16501 gtccgtttgg gtaactacga ttgggactgg ttgtaaaatc aatccccttg acctctgcag
    16561 cttccaattc ttttttgata gacatcatat tggcataggt aatcccattt ccctttgctc
    16621 caaaggaaac ttgcttgaga ttaggttgcg agagttgctc tcttacatag gattcttcca
    16681 tgtccagata cttatgaaag acctctgcaa ccttgttaaa ttgtgttttt tctacgtaaa
    16741 gaatcttacc cgttgctgac ttatagttct catcaatgac cgcatagaca ttataggagg
    16801 ttgcatcctc agcaatcggg actccatttc ggtcataaat agtcccacgt ttggcaggaa
    16861 ctgtacgggt ggtttgatga accttcttag cttccttcgc taaatctgtt ccaaagcgag
    16921 tgcctgtccc aataatgacc gcaaaattga ctaaaaaaat ggcaaaaaca aagacagata
    16981 ataaactcag actttttcca actctgcgtc tgttttcagc cggcgatttc cgatttttgg
    17041 tcgcataacg gattactctt tttgtccact tcatatctta ctccgctatt ctaatatttt
    17101 cattgtttaa ttgcaaatcg tgtgaattgg caatttcttt caaacgttct gcacgtaata
    17161 gttcattgac ctcttgcttg gcatcgtcca attcggtttt cttttcctct atctgcgcat
    17221 tgatttttgt caaatcattc tgcacttgca agagcttggt ctgcataaaa ataatactaa
    17281 tggctacaat aagagtggtt acagcaatgg aaaagtaaaa agctttttcc acacgcgaaa
    17341 accgtttaag ttgcatctgt agtatttgac ctgttttttc cattttttct gccatctttt
    17401 tccctcttac ttgtgaattt ttctggccac gcgcaacttg gctgagtgcg agcggttatt
    17461 ggcttctaac tcttccgcac ttggcaagat tggcttacgg gacaccaatt ccatcttggg
    17521 cttgagatca tctgggatga aaggcaagcc ttttggaact tcaactgttg aagcttcctt
    17581 gaacaattgc ttggtcaagc ggtcttctaa ggaatgaaag gtaatcactg aaattctacc
    17641 atccagagcc aacatatcca tagcctgctg gatggactca tctgccgctc ccagttcatc
    17701 attgacttca attcgaatag cctggaaaat ctgcttagca ggatgaccct tcttcttgag
    17761 ttccttggca ggtttgacca acttgataat ctctgctaac tcagtcgttg tctcaatcgg
    17821 cttcacttca cgcgcttgct caatcttacg cgcaatctgt ttagagaatt tatcctcacc
    17881 gtatttgaag aaaatacgaa ccaaatcatg atagtcatag tgattaacca cttcataggc
    17941 tgtcagacta gcatcctgat tcatccgcat gtccagtggc gcatcctttt tataagaaaa
    18001 accacgctca cgctggtcca attgaggact agacactccc aagtcataac aaattccatc
    18061 aatttcctga acaccagctt cgcgcaaacg tgcctgtaaa tgacggaagt tatccttgat
    18121 aaaggtcacc attcccttct caatgtaagg tgccaagcgt ttttgcgcat tgtcaatggc
    18181 attctgatcc tggtcaaagg catagagatg gcctttttca cttaatttac ttaataaata
    18241 ctcgctatgg cctgctccac ccaaagtcgc atcaacgtag ataccgtcag gttttacgtc
    18301 aagcatatca atcgtttcgt ggagtaagac cgttacatga tgaaattctt ttgtcatatc
    18361 ttatctattt taccacaaat ccgaccagct tgcacttgtc agacaaatag gacaaggaga
    18421 ctactatttc ttcaaaaaat atgtcaaaaa tacttgacac tttatgttta aaagactata
    18481 atatagtcaa atatatacga cataaatcag aaaggagacc agatgaatcg tgtgaaagaa
    18541 tttcgcaagg aattgggtat ttcccagctc gaactcgcca aggatatcgg tgtctcgaga
    18601 cagactatca atatgattga aaacgacaag tacaatccaa ccttggaact ctgtctcaat
    18661 ctcgcccgca gcctccaaac tgacctcaac agtctctttt gggaggaaga tttttaaaaa
    18721 aggagcaaac tcatgaaaaa agaaaccttc actgaaaaac tgatcaaacg cacatacggt
    18781 atttctggtc cccttgacga atacaaacgg cgtgaggccg atcgtattgg gaaccaagtc
    18841 tttatcatcc tcttttatct gatgattttc ggaaatctta ttccactcct tctggcctat
    18901 aaataccctc aagaagtggc tctaatctat cctcctctga ttttagtgat tgccctcatc
    18961 gctgctggct atgtcaccta ccaaatgaaa aaaacaggga ttacagctat tgatccagac
    19021 atgctgagcg agaaagaaag taagcaacta cattacccag gtctgaaagc aggtttgttc
    19081 tttggtctat ggatgttttt tataactcct cttctcagta tactcataga tgaaggtcag
    19141 gactattttc attctcttct cactataaga aatggtgtat caagtattct cggttctatt
    19201 ttcttcggag cgagcataca gttcctcatc tcccgtcgca ttgcaaaaac taagaaaaat
    19261 caagatgagg attaggagga aactcatgaa aaaagaaaag aaacagttac gttatcctgg
    19321 tctgaaagca ggttcgattt atggaacagt gatatttttt ataattccac tcattgatac
    19381 cctaacaagt gaaaatccaa attttatcag ttctcttcta aatacaaaac atatttttaa
    19441 aactatactg ggagctttct ttttcggagt gatgatacat attgtcgact cacttcgtat
    19501 tgcaagagct aaaaaagacc aggattagga ggtgccttat gaaatcacta gctagactac
    19561 tgatcattca tgtttttatc agtattttcc ttttcttcgc ccttacttca ggagctattt
    19621 ctcatacagt tttactactc ctactcctct ttcttcctgc gctcaataaa ggacttgaga
    19681 aaatacaatc aaaacggata cctgtcctca acgcagccct cttctttctc ctcatatcct
    19741 ttccacaact tttaaccaac cctgtccaat ggaaattttc aatattccta gtcgtaacca
    19801 tcatttcaag tttggcctac ttctataact tttatcaagt agttaaagaa gtagatcaaa
    19861 aacagttgat ttaggagctt gctcccataa tttctcattt cataggcaaa taatcccacc
    19921 tagcttgttc taggtgggat tattttattt cttaacagaa attgcaagtc attttccttc
    19981 attttagaaa gtcgactctt tccaattcac actacaatcc aagacttgtt gcctttcttc
    20041 ttgattgcgg ccttcaatct gatcaatcaa aatctttgta gcctgttgtc cttcctcaaa
    20101 ggagggctga accagcgtcg aaacacttgg agaagaaaag caagtccact ccgtattgtc
    20161 aaaaccaatc aacccaactt gcggcaagtt ataattcaac tctttgataa cggtaaagac
    20221 tagaggtagg gcccaacagt tagggataaa taccagagtt ttttcatcgg gatcgatttc
    20281 tttttgtaaa aattctttaa tttgttccaa attcgtatgc ttatcttcaa tggttagact
    20341 ggcgtgacgc atattagcat ctgttaaagc atccacaaaa ccacttgccc gctcaatccg
    20401 agtactcaaa cgactcgtat ccgctgtaat caagagaaaa tgctcataac ctttttcgat
    20461 acaggactgg gtcatgtcat aaacggcatc atagttattg gttttaaccc agctagtccg
    20521 gtgttcatag agctgactat caaaaaagac cattttcttc tttttctcat cgatgatacg
    20581 agaatatttt cggaaattag aggtcggctg aataataaag ccgtctactc ccaagagaag
    20641 catgctttca atataccggt cctcactctc ttggctgtaa ttgctatttc ctatcattac
    20701 ctggtagcca ttctggctgg cgatatcctc aattccctta acaatttggt ttgagaaact
    20761 gttggtaata tcaccgatca aaacaccgat taattttgtt cgtttggagt ttaagctacg
    20821 cgcaacaatg ctcggtttgt aatttgtttc atgaataact ttttcaatct tttcacgtgt
    20881 ctcttgggac attttttcat atttcccgtt taggtaaaat gacacggttg ttttcgaggt
    20941 ctgagccatt tccgcaatgt cttttatggt cagtttcttc tccaatctaa aacacctcct
    21001 actaatactt attataacat attcactatt caaaatgcct atttctagca ctgcaagcga
    21061 atcattctct catttatttt atcatatact agacattttc cacgcatttt tgttccgtca
    21121 acgaggcaca atttttcacc tacacaaata tcctcgctta ataacagaat cagcttgtct
    21181 gtttcgtcat ttttcacttc aaaagccaga gcattttcca cttcacgatc atctgtctgg
    21241 tagacagaat gacggatgat ttcaaaactc tcatgcgcaa tgatggtata atccagcatc
    21301 tgattctcaa agaattggcg cttggtgagt ttgttacttc tttcaagctc attgtattta
    21361 ggagaaatga tggtatcttc caaatcaaaa tcaacttcac tccatagtct caactgattg
    21421 attttcccat cttgataggt cacatccttg tcaaggataa actgagtcaa cgcctcatgc
    21481 tgaccttgac acctgatgtc atctaccaag agccagacat cctctaccaa catgaggatt
    21541 tttctcttgt gaagataagg caaatcaggt tctgctgacc aataagcccc ctcaatataa
    21601 tgcactccct ccctttcttt atggtgacaa aacagggagt gaggatagta ttcatattcc
    21661 caggatcccg tgattctttc cggagctttc ccatctacaa tgcaggtcga atgactccaa
    21721 gcactcttta agagataacg ttcatatatc tcccgataag aataacgccc agcatctatg
    21781 aaaataggtt ggccttgata ctgtaaacaa aaactattct cgtcactatg gctatgggca
    21841 cttcctagcg gaccattttt gaaaaataga taacgatgtt catccttaat gcagacatgt
    21901 ccagagtctt caaagatcat ggacttaggc tgccaagctc tcctttcaaa ttcctgcagt
    21961 cgcttgacct tttctcgccc caggaacaag aggctaagca aatcgacttt aacatccaga
    22021 ccgttaagaa ggtcttcctt gttcaaaacc acagcagaca ggctcaaaat ttctgtcgtt
    22081 tctgtagaat cgctatcacc aaaagccaaa gttcgtccat ctaagcctgt catcatttga
    22141 atataggtcg ccatcttttc cagcaactct cggtaactat cttgcaagtc tggaagcaag
    22201 agacacaaat ccagcaaggc tttataaacc tctacatgat agagaatcga ctgttcaaac
    22261 tggcttccat ctcctaaaat ctgtgtctca atttgctgtt tcaactcctc tgaagcaaaa
    22321 tggtaagctt cttctagatc catcttatct gaaaagaaat gatagatagc aagcatcgga
    22381 attgtttgta aaatccccca gttactaagg gtgtacttgg cgcgatagta gcttttcata
    22441 aagtcaatct gcttttctag actgaccaaa attttctcta attctttctc ctctagcaag
    22501 tcaaatttca agaggagcaa gagtagtttc aaccaagtaa aggaacgaat acccgtatcc
    22561 aaggttctag tcatcaagga ttgaggagaa aattctctca cctgctcaat ccaatcaaat
    22621 agaaagaact tgcacttttg aatatagtcc ttatctcctt ctaccagata ccctatcata
    22681 aactgcaaga gatattcttg tcgattgagc atataagacc attctggatc atcttcaaat
    22741 acttgatccc ataccatcgg ctggatttga tggatttttg aacaaggctc catatcccaa
    22801 ggactatcaa acataaaacg attgtccatc aagcgttcaa gggaactctt gactttctca
    22861 tagtcttttg aacagtggga caagatataa tcacgacatt ggtttccatc gactctttca
    22921 aaaaattgtc ttctttcttc tttcattatc tattaccaga aaaagaacta cttaaaaagc
    22981 agttcttttg tctttcccat tacactttcc ttttctacat agatgaccac accttttgca
    23041 atctgcaagg agaccaagtc atctttgata gaaatgattt ttccatgaat tccagacaat
    23101 aacaacactt catcaccaga tgttaaagaa gctaaatact cttgtcgttg ctccatctgt
    23161 ttgcgaagca acttttgctg acgaatagaa tgaaagcttg acagtaaaag gggactcact
    23221 gccaagacaa tcactattcc ataaaacaat gttgtatcca ttaagctata atcttaagcc
    23281 agcttccgat aattccgatg ataactgtta aaataacgag tttatatgtt gtccatttct
    23341 tttctttgat caagtagtaa actaaaagtg taaatagggc tggtagaaga gctggagcaa
    23401 ccttatcaag cattccctga atacttacga tactttgttt agcgtctgct ttaacttccc
    23461 ctgcagcaaa ggtaatcggc accataatct taacagatgt cgctgccaaa ccagcaatta
    23521 cggttacacc gataatattg gcaatacgag aaatcgttgc catctgttcg cttagtttat
    23581 caatcacagt tgttcctagt ttgtatccat acagaccagt tgacaattta atcgctgtta
    23641 aaatcgtatt catcgcaagg aagaacaaga ttggaccgac aaccaagcct tcttgagcaa
    23701 acgaagctgc gatggttgag aacaatggag ctaaacagaa ttgagaaaga gaatccccaa
    23761 tacctgccaa tggtcccatc aaggccatct tgatgctacg tgtttctttt gttggtcggc
    23821 cattttccaa cattacaaga tgcaagctgg taataaaagg caggaagtgt gggttggtat
    23881 tatagaattc acagttttct tccaaggctt ggtagaaacc ttcctgatcc tctccatagt
    23941 gttttttcaa agcaggatac atcacattgg catatcccaa cccttgatag ttactatagt
    24001 taaatccatt ttgacaaaag aatgcccgca aagacgtttt aagataatca cgttttgtta
    24061 atttgttaga tccagtcatc gtgtgcttcc tcctctacca catgatccgc tgtttttggc
    24121 ttgttataaa attcaatcaa agcaaagata gtacctacaa ttgcaatacc aattgttggg
    24181 atgtttagat aagctgcaca aacatatccc aacaagacaa agggaatcaa ctctttctta
    24241 gccatcactg acaagatcat cgcaaaaccg atagctggga gcattttacc agcaactgtc
    24301 aaacctgtaa gcaatactgg tggaatgtag tctacgagtt tcaacaaggt atccattgaa
    24361 agggcaccca agcaacccaa ggtaaatcca ataaaggcaa acaaccaaat tgttgcattt
    24421 agagtgaact tgaatttctt caaattatgg tttttcaagt gcttcatcgc cgtttcaggc
    24481 gcaccagcac gtacagtgta ggcgaaagtt tgtaagaatt gaatcgccac agcaatcgga
    24541 gtagagaggg caagagccgc ttctggactg actttaccag cactagtgat agccatcaag
    24601 gtaccaaaga taccaggtcc gattgggttt ggtggaacag tacctccagc accaacaccg
    24661 aatcccatat aagccaattc accaatagct cccattgcaa gagcagtagg caaatcacct
    24721 agaataattc cgacaccaaa tgacagaaca atacatctat tggtgtaaat tcctaacagc
    24781 attccactaa aacagaaagc tgtccataat ccaattaaaa tcgcttgaaa tacattaatc
    24841 gacataacga tctcctcttt taaatatagt ccataatgtt gacttgaaca gctccatcat
    24901 ttcctgttgg agttgttttc gtattaaatg ttacttgatg agtttggctc aattccttga
    24961 gggccgcctt gtcctcttca cccaggaaga tggagcgtgt cacttgctct ttaccagtag
    25021 cattgtgaat gttcccaata ttgatttctt tgatagggac accaccttct accaaggtta
    25081 aagcgtcctt cacatccttt acaacgataa agatcgtttg agcaggatta gccttgtgaa
    25141 tgatatcaat caccttttgc aaagggaaga aacgcatggc aactgagtct ggcacaactg
    25201 ttttcatcag agtttgttgc atcttgtctg tgcttacttc gtcattggca acaatgaccg
    25261 tattacaacc taggtatttt acccaaagtt gtccttgccc atgaatcaac cgttcatcga
    25321 tacgggtcat aataatattt ggcattgtca tatttctcct cctaccaata taggttccag
    25381 tctttgtaga aacggataag ggcttctagg taatagtagt caccccagat attgccttca
    25441 tccactcctt tacctgaatg ccatgagtac acaccgtgga ggagacttgt cccaccaggg
    25501 gtaaattgat catttgcata atgttcgatc aaggaacgaa gcatggcatg catagcatgt
    25561 ttataaatat ctttgtcagc atccacctct gggagatgtt ttagcatttc atgaatccca
    25621 cagacggcga tagctgttgc tgaagaatct cgtgattgat cactaccatc attaaaaatc
    25681 aaatcccaat aggacacatg atcttttggc agacgattca agaaataatt ggtcacaccc
    25741 ttaaacaagt caaagcagga ctcgtctttt aagtgacgat aagtcaaagg aataccatag
    25801 actccccatg attgaccacg tgcccagcat gaatcatcac tatacccttg tctcgttaca
    25861 cctttaaagg gttgacctgt ctcaggatca aaatagaagg tgtggaagga cgaagcgtca
    25921 tcacggatta cattattagc tgaagcatag aaatggcttt ctgcaatatc gtagtatttt
    25981 tgatcgcctg tttcttgata agcaaagaat aagagttgga tattgagcaa gcagtcgata
    26041 atcaaacggt aatgctcttt cttgcccaag tctccccaag cttgaataaa accacctttt
    26101 tcttgatagc gttcaatcaa cttatctgca gctttcaagg ttgcttctct agcctctcca
    26161 tctccattta tcttatattc agccatacaa gacggtgtgt acaagaagcc gagatcatga
    26221 tgatccaatt ctactctctt attgacacga tccaggaaag aaagaacatt tttatgagcg
    26281 atgtttttaa atgcatcctg ttgactgtat tcataagcca accacagttc tcctgtccag
    26341 aaaccattgg tccattccgt gttatccatg attggataga cattatcaaa ggtagctggc
    26401 gtcgggaaat cttccttgaa atagtcaagg ttgagttcta actgccgaat aaccttatcg
    26461 attgcctggc cgacttcttc tttcgtcaga agtggtactt ctaagaagcg ctcaggcgat
    26521 tttatttttt caatcgtaac cttttttatc attcttttac cctttaaata cccgattcga
    26581 aatcttcttc ctcttcctct gcatccgttg aaaacaattc tttaccattt acgactccgc
    26641 cctgggccgc cactactgat ttattaacaa cctcatcaaa gctatgagcc attctagcaa
    26701 agactgcttc cattaacatg gctaagttca aacccgagag aacattcatt gttttgactg
    26761 gattttctcc cattatggta gaagaaacct tgaatggcga tcctcccaag agatcactta
    26821 ggattaaaac ttcttcttca tttgaaattg caagtaagat tttttgcttg agttcatctg
    26881 ctgacattcc ttccacaaag tcaatcgcct ccacattttc ttgattgcct gcaatcaatt
    26941 gtaaagaact ataaatccct gtagcaaaat gtccatgccc tacaagtaca attttcatct
    27001 tttctccttt cttgtttagg tttaccggtt tactttatat t
//
LOCUS       DAVMAF010000019        26820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMAF010000019 DAVMAF010000000
VERSION     DAVMAF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.019"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..271
                     /locus_tag="VYO97_000846"
     CDS             <1..271
                     /locus_tag="VYO97_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1753668.1"
                     /translation="EKPKPEVKPQLEKPKPDNSKPQADDKKPSTTNNLSKDKQPSNQA
                     STNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(490..1470)
                     /gene="dusB"
                     /locus_tag="VYO97_000847"
     CDS             complement(490..1470)
                     /gene="dusB"
                     /locus_tag="VYO97_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1753669.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGEDPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1457..2329)
                     /gene="hslO"
                     /locus_tag="VYO97_000848"
     CDS             complement(1457..2329)
                     /gene="hslO"
                     /locus_tag="VYO97_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1753670.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2494..3921
                     /locus_tag="VYO97_000849"
     CDS             2494..3921
                     /locus_tag="VYO97_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1753671.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4080..5588
                     /gene="glpK"
                     /locus_tag="VYO97_000850"
     CDS             4080..5588
                     /gene="glpK"
                     /locus_tag="VYO97_000850"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1753672.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFESGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5630..7456
                     /gene="glpO"
                     /locus_tag="VYO97_000851"
     CDS             5630..7456
                     /gene="glpO"
                     /locus_tag="VYO97_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1753673.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7526..8230
                     /locus_tag="VYO97_000852"
     CDS             7526..8230
                     /locus_tag="VYO97_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1753674.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8527..8730
                     /locus_tag="VYO97_000853"
     CDS             8527..8730
                     /locus_tag="VYO97_000853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753675.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8817..9425)
                     /locus_tag="VYO97_000854"
     CDS             complement(8817..9425)
                     /locus_tag="VYO97_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753676.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGTGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10514..11791
                     /gene="ltrA"
                     /locus_tag="VYO97_000855"
     CDS             10514..11791
                     /gene="ltrA"
                     /locus_tag="VYO97_000855"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1753677.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYFRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     YFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGFLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12247..13425
                     /locus_tag="VYO97_000856"
     CDS             12247..13425
                     /locus_tag="VYO97_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1753678.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            13739..13870
                     /locus_tag="VYO97_000857"
     CDS             13739..13870
                     /locus_tag="VYO97_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1753679.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13891..15441
                     /gene="dltA"
                     /locus_tag="VYO97_000858"
     CDS             13891..15441
                     /gene="dltA"
                     /locus_tag="VYO97_000858"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1753680.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15438..16682
                     /gene="dltB"
                     /locus_tag="VYO97_000859"
     CDS             15438..16682
                     /gene="dltB"
                     /locus_tag="VYO97_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1753681.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16696..16935
                     /gene="dltC"
                     /locus_tag="VYO97_000860"
     CDS             16696..16935
                     /gene="dltC"
                     /locus_tag="VYO97_000860"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1753682.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16928..18196
                     /gene="dltD"
                     /locus_tag="VYO97_000861"
     CDS             16928..18196
                     /gene="dltD"
                     /locus_tag="VYO97_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1753683.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18351..18791
                     /gene="adcR"
                     /locus_tag="VYO97_000862"
     CDS             18351..18791
                     /gene="adcR"
                     /locus_tag="VYO97_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1753684.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18791..19495
                     /locus_tag="VYO97_000863"
     CDS             18791..19495
                     /locus_tag="VYO97_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1753685.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHNAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19488..20294
                     /locus_tag="VYO97_000864"
     CDS             19488..20294
                     /locus_tag="VYO97_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1753686.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20304..21809
                     /gene="adcA"
                     /locus_tag="VYO97_000865"
     CDS             20304..21809
                     /gene="adcA"
                     /locus_tag="VYO97_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1753687.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22938..23712)
                     /locus_tag="VYO97_000866"
                     /pseudo
     CDS             complement(22938..23712)
                     /locus_tag="VYO97_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            23888..25291
                     /locus_tag="VYO97_000867"
     CDS             23888..25291
                     /locus_tag="VYO97_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1753688.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIAETQYYYPQLS"
     gene            25320..26612
                     /locus_tag="VYO97_000868"
     CDS             25320..26612
                     /locus_tag="VYO97_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1753689.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLVAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVQTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
BASE COUNT         8078 a         4921 c         5819 g         8002 t
ORIGIN      
        1 ggaaaaacca aaaccagagg ttaaaccgca gctggaaaaa ccaaaaccag ataatagcaa
       61 gccacaagca gatgataaga agccatcaac tacaaataat ttaagcaagg acaagcaacc
      121 ttctaaccaa gcttcaacaa acgaaaaagc aacaaataaa ccgaagaagt cattgccatc
      181 aactggatct atttcaaatc tagcacttga aattgcaggt cttcttacct tggcgggggc
      241 aaccattctt gctaagaaaa gaatgaaata gtgattgatt cattcaaaaa tgtttgtaaa
      301 aagtattcgg cttaaaagaa aagagtctgg gataaaaaga ttttgatttt aggaattctt
      361 tattataaat ttttaaaatc gatagattag acaaaaaagc gaacaagaca gaattctgag
      421 tgtcagataa ctgattttgt tcgcttttta tatttaatat tatacttttg tcctaggctc
      481 ttttttgcat tatacttttt ccaattgcaa gagagcttca atctctgcta gggtgctagc
      541 ttgcgaaatg gctccacgga gtttggcagc gccagatgtt ccacggagat agtgaggagc
      601 gaggccgcgg aattcacgaa ctgcgacgtt ttctcctttg aggttaatca atcgtttcaa
      661 gtgttcgtag gcgatcttca tcttgtcttc aaatgtcaaa tcaggtagga tttctcctgt
      721 ttcaaagtaa tggttgattt ggttgaagag gtaaggattt cccatggcag ctcggccaat
      781 catgactgcg tcagcaccaa cttcttcgat gcgttgcttg gcttcttgga cagtacggat
      841 atcaccgttg gcgatgaatg gaatcttggt tagagcttgg gcaaccttgt aaagggtctc
      901 aaggtccgcg tggccagtat acatttgttc acgggtacgg ccatgcatgg cgagggcaga
      961 aacacctgca gcctcagcag cgagggcatt ttctactgca agagatgggt ccgcccagcc
     1021 ggtacgcatt ttgacagtaa gtgggatatc aaggacagac tggaccttgt tgatgataga
     1081 gtagatcttg tcaggatcct tgagccacat agcaccagct tcgttcttca cgattttgtt
     1141 gacagggcag cccatgttga tatcgacgat atcggtctta gtgttttctt ggataaattc
     1201 tgctgcgcgt gctaggctgt cttcatcgct accaaaaagt tggatagaga cagggtcttc
     1261 gccctcatcg atatgaagca tgtgcagggt tttttcgttg ttgtattgga ttcccttgtc
     1321 agagaccatt tccattacaa cgagtccagc tccgagctct tttgcgatgg tacgaaaggc
     1381 tgagttggtc acgccagcca taggcgctaa aacggtacga ttgggaatct caacattgcc
     1441 aatcataaaa ggtgtattaa gatttgtcac gaatgagttc ctccaggtcc ttttcatcaa
     1501 agttgtaagt agtttggcag aattgacaag tgatttctac cccgtggtct tcctctttca
     1561 tttcctgtaa gtctgagctt ggaaggctgg caagagcgtt cataaagcgt tcatggctac
     1621 agtcacattg gaaacggatt tcttcttcag aaagacgctt gtaggcttcg tccccgtaga
     1681 tagccttgag gagggcttcg atatggtcgt cgctttcgag aagagtagag atagctggca
     1741 tttcttggat gcgtttttca aagcgagcaa tctcttcttt cttggctcct ggcaagactt
     1801 gaactaggaa accacctgca atcttgacct tgtcttcctc gtccaaaagg acattgaggc
     1861 cgaccgctga aggcgtttgt tggctttcag taaggtaaaa ggcaaggtct tcaccgattt
     1921 ctccagagat gaggggagtt atagagttgt aaggatttcc agtaccgtag tctgtgataa
     1981 cgaggaattg accatttcca acaaaaggtc cgactaggac ttcaccagtc gcagtctttt
     2041 tgatgtcaac accaggattt tgaacgtagc ctttgacgtt ccccttggta tcagcgacgg
     2101 tgataatagc acctagagag ctagatccca acaccttaac tgtaagtttg gtatttcctt
     2161 tttcattggc tgcgagaatc tggctggcga taagagttcg accaagcgct acagttgagc
     2221 tagcttgggt ttgatgtttt tcttgagcag tgcggacggt ttcagtgcta tcaaggacaa
     2281 aagcacgaaa ggctccgctt tctgatatag ttttaataat tttatccata gctactattt
     2341 tagcataaaa atgcccaaag ggggagccgt gtgtttactg attttcagga taatggacca
     2401 ggaaatcagc atgaaaataa aaagagaaac agattatttt agcatttgta agatttatgc
     2461 tatgcttaag gtagaaaatg aaaggggtaa caaatgtatt taggagattt gatggagaaa
     2521 gccgagtgtg gtcaattttc aatcctttcc tttctattac aagagtctca gacgaccgtc
     2581 aaggctgtaa tggaagaaac aggattttca aaagcaaccc taaccaaata tgtcaccctg
     2641 ctcaatgaca aggctttgga tagtggctta gagctgacta ttcactcaga agatgaaaat
     2701 ctgcgtctgt ctatcggtgc agctaccaag gggagagata ttcggagctt gtttttggat
     2761 agtgctgtta aataccagat tttggtttat cttctctacc accaacagtt tttagcccat
     2821 cagctggctc aagaattggt gattagcgag gctacgcttg gtcgtcactt ggctggttta
     2881 aatcagattt tgtcagaatt tgatttatcc atccaaaatg gacgctggag aggtccagag
     2941 catcagattc gctatttcta tttctgtctt ttccgaaagg tctggtcgag tcaggaatgg
     3001 gaaggtcaca tgcagaaacc agagagaaaa caggagattg ccaatttaga ggaaatctgc
     3061 ggtgcaagtt tgtctgcggg gcagaaattg gacttggttc tctgggctca catcagtcaa
     3121 caacgtcttc gggtcaatgc ttgtcagttt caagtcatag aagagaaaat gcgagggtat
     3181 tttgacaata tcttttatct tcgtttgctg agaaaggttc cgtccttttt tgctgggcaa
     3241 catattccac taggagttga ggatggtgag atgatgatat tcttctcttt tctcctatct
     3301 catcgcattc ttcctcttca tactatggag tatattcttg gttttggagg gcagttggca
     3361 gatttactga cgcaattgat tcaagaaatg aagaaggagg aactattggg ggattataca
     3421 gaggaccatg tcacctatga actcagtcag ctttgtgctc aagtctatct ctataagggc
     3481 tatattttac aggatcgcta caagtaccag ttagagaatc gtcatccata tttactgatg
     3541 gaacatgatt ttaaagagac agcagaggag atttttcatg ctctgcctgc ttttcaacag
     3601 gggacagatt tagataagaa gattctctgg gaatggctcc agttaatcga atatatggct
     3661 gaaaacggtg gccagcatat gcggattggt ctggatttga catctggttt tcttgtcttt
     3721 tcaaggatgg cagccatttt gaaacggtat ttggaataca atcgttttat taccattgaa
     3781 gcttatgacc ctagtcggca ttatgatttg ctggttacca ataacccgat tcataagaag
     3841 gaacagacac cagtctatta tttaaaaaac gacttggata tggaagattt ggcagggatt
     3901 cgtcagttat tattcactta aaaggcttgg ttaatccagg tcttttttgt gaaattcaca
     3961 caatctcctc acattttttt aaaaattaaa aaaagttgat aaacaagaaa gcgctttatt
     4021 ttgtatacta gtaagtgtaa agaggaaaca cctcaagatc tttatcagga ggacagtaca
     4081 tgtcacaaga aaaatacatc atggccattg accagggaac tacaagttct cgtgccatca
     4141 ttttcaacaa aaaaggggaa aaggttagct cgagtcaaaa agagtttacc cagattttcc
     4201 ctcaggcagg ttgggttgag cacaatgcca atgaaatttg gaactctgtt cagtcagtta
     4261 ttgcgggtgc tttcatcgaa agtggtgtca agccaaatca aatcgaggca atcgggatta
     4321 ccaaccaacg tgaaacaacg gttgtctggg ataagaaaac aggacttcct atctacaatg
     4381 ctatcgtttg gcagtcacgt cagacagcac ctttggctga gcaactaaaa agccaaggtt
     4441 atgtggaaaa attccatgaa aagactggtt tgattattga tgcttacttc tctgctacca
     4501 aggttcgttg gattttggat catgtagaag gtgctcaaga gcgagcagaa aaaggggaat
     4561 tgctctttgg tactatcgat acttggttgg tttggaaatt gactgacggt gcggctcacg
     4621 tgactgacta ctcaaatgca gctcgtacca tgctttataa cattaaagaa ctcaaatggg
     4681 atgatgagat tttggaaatc cttaacattc cgaaggctat acttccagaa gttcgttcta
     4741 actccgaaat ctacggcaag acagctccat tccatttcta cggtggagag gtgccaatct
     4801 caggtatggc tggggaccaa caagcagccc tctttggaca gttggctttt gagtcaggta
     4861 tggttaagaa tacttatgga acaggctctt tcatcatcat gaatactggg gaagagatgc
     4921 agttgtctga aaacaacctc ttgacaacca ttggttacgg aatcaacggt aaggtttatt
     4981 atgccttgga aggttctatc ttcatcgcag gaagtgctat tcagtggctt cgtgacggtc
     5041 ttcgcatggt tgaaaattca ccagaatctg aaaaatacgc tcgtgattct cacaacaacg
     5101 atgaagttta tgtcgttcca gcctttacag gtctaggcgc tccatactgg aaccaaaatg
     5161 ctcgtggttc cgtctttggt ttgactcgtg gaacaagcaa agaagacttt atcaaggcga
     5221 ctttgcaatc tattgcttat caagtgcgtg atatcatcga caccatgcaa gtggatactc
     5281 agaccgccat tcaagtactg aaggtggatg gtggtgcagc catgaacaac ttcctcatgc
     5341 agttccaggc ggatatttta ggcattgata ttgcacgtgc taaaaacttg gaaacaacag
     5401 ctctaggagc ggccttccta gcaggtttat cagtagggta ctggaaagac ttggacgagt
     5461 tgaaactctt gaacgagaca ggagaactct ttgagccatc tatgaatgaa tctcgcaagg
     5521 aacaactcta caagggctgg aagaaggctg tgaaagcaac tcaagtcttt gcggaagtag
     5581 acgactaata ctggcagaat aaagcgattt atttagaaag tgtgtaaata tggaattttc
     5641 aaagaaaaca cgtgaattgt caattaaaaa aatgcaggaa cgcaccctgg acctcttgat
     5701 tatcggtgga ggaatcacag gggctggtgt agccttgcag gcggcagcta gcggtcttga
     5761 gactggtttg attgaaatgc aagattttgc agaaggaaca tctagtcgtt caacaaaatt
     5821 ggttcacgga ggtcttcgct acctcaaaca attcgacgta gaagtggtct cagatacggt
     5881 ttctgaacgt gcagtggttc aacaaatcgc tccacacatt ccaaaaccag atccaatgct
     5941 cttaccagtt tacgatgaag atggagcaac ctttagcctc ttccgtctta aagtagccat
     6001 ggacttgtac gacctcttgg caggtgttag caacacacca actgcgaaca aggttttgag
     6061 caagaaagaa gtcttggaac gccagccaaa cttgaagaag gaaggcttgg taggaggtgg
     6121 agtgtatctt gacttccgta acaacgatgc gcgtctcgtg attgaaaaca tcaaacgtgc
     6181 caaccaagac ggtgccctca ttgccaacca cgtgaaggca gaaggcttcc tctttgacga
     6241 aagtggcaag attacaggtg ttgtagctcg tgatctcttg acagatcaag tatttgaaat
     6301 caaggcccgt ctggttatta atacaacagg tccttggagt gataaagtac gtaatttgtc
     6361 taataaggga acgcaattct cacaaatgcg cccaactaag ggagttcact tggtagtgga
     6421 ttcaagcaaa atcaaggttt cacagccagt ttacttcgac acaggtttgg gtgacggtcg
     6481 tatggtcttt gttctcccac gtgaaaacaa gacttacttt ggtacaactg atacagacta
     6541 cacaggtgat ttggagcatc caaaagtaac tcaagaagat gtagattatc tacttggcat
     6601 tgtcaacaac cgctttccag aatccaacat caccattgat gatatcgaaa gcagctgggc
     6661 aggtcttcgt ccattgattg cagggaacag tgcctctgac tataatggtg gaaataacgg
     6721 taccatcagt gatgaaagct ttgacaactt gattgcgact gttgaatctt atctctccaa
     6781 agaaaaaaca cgtgaagatg ttgagtctgc tgtcagcaag cttgaaagta gcacatctga
     6841 gaaatatttg gatccatctg cagtttctcg tgggtctagc ttggaccgtg atgacaatgg
     6901 tctcttgact cttgctggcg gtaaaatcac agactaccgt aagatggctg aaggagctat
     6961 ggagcgcgtg gttgacatcc tcaaagcaga atttgaccgt agctttaaat tgatcaattc
     7021 taaaacttat cctgtttcag gtggagaatt gaacccagca aatgtggatt cagaaatcga
     7081 agcctttgcg caacttggag tatcacgtgg tttggatagc aaggaagctc actatctggc
     7141 aaatctttac ggttcaaatg caccgaaagt ctttgcactt gctcacagct tggaacaagc
     7201 gccaggactc agcttggcag ataccttgtc ccttcactat gcaatgcgca acgagttggc
     7261 tcttagccca gttgacttcc ttcttcgtcg taccaaccat atgctcttta tgcgtgatag
     7321 cttggatagc atcgttgagc cagttttgga tgaaatggga cgattctatg actggacaga
     7381 agaagaaaaa gcaacttacc gtgctgatgt cgaagcagct ctcgctaaca acgatttagc
     7441 agaattaaaa aattaagaaa aaataaaaga ggtggagggc agcattcctt gtcgcccgtc
     7501 ccttcttttt aatggagaca gaaagatgat gaatgaatta tttggagaat ttctagggac
     7561 tttaatcctg attcttctag gaaatggtgt tgttgcaggt gtggttcttc ctaaaaccaa
     7621 gagcaatagc tcaggttgga ttgtgattac tatgggttgg gggattgcag ttgcggttgc
     7681 agtctttgta tctggcaagc tcagtccagc tcatttaaat ccagctgtga cagttggtgt
     7741 agctttaaaa ggtgacttgc cttgggcttc cgttttgcct tatatcttag cccagttcgc
     7801 aggggccatg ctgggtcaga ttttggtttg gttgcaattc aaacctcact atgaggcaga
     7861 agaaaatgca ggcaatatcc tggcaacctt cagtactgga ccagccatca aggatactgt
     7921 atcaaacttg attagcgaaa tccttggaac ctttgttttg gtgttgacaa tctttgcttt
     7981 gggtctttac gattttcagg caggtatcgg aacctttgca gtgggaactt tgattgtcgg
     8041 tatcggtcta tcactaggtg ggacaacagg ttatgccttg aacccagctc gtgaccttgg
     8101 acctcgtatc atgcacagca tcttgccaat tccaaacaag ggagacggag actggtctta
     8161 cgcttggatt cctgttgtag gccctgttat cggagcagcc ttggcagtgc ttgtattctc
     8221 acttttctag tttatactct tcgaaaatca aattcaaacc acgtcagcgt cgccttaccg
     8281 tactcaagta cagcttgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
     8341 gaaaacaatt atgttgatag agcttgggca agagcccaat ttcagcaaaa aatgaagtaa
     8401 atcttctcat aataaaacgc atcatatcaa gcacgaaaat tccacgaggt caactacagt
     8461 cagaaagctg aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca agcacctgca
     8521 agcaacgtgc cgaaatggtc aaatcctgat tatgtcaacg aattagaccc aaaaatcgtt
     8581 gatatgctag tagaatttca caagtcacaa ggcactttgg aaactcccga ggcgcaagca
     8641 gaaatcgccc aaaaacgtga agaaatcgag caaaggagag ctgagcttga ggataaaaaa
     8701 caagagcttt tgaaccgctt gaacaaatag agtttcgcaa gtattatgct tacaaattac
     8761 ttgagcaatt aactaaaata taaaccctgc ctttatatct aggcagggtt tatattttag
     8821 aaattcacgt aggttgttac ggtttttaca tacccagtat agtttgagtt tctatagtat
     8881 tcagtgataa acttccattt tctttgagca acatggatat aagtacttgt tatgtagtat
     8941 ggatatgggc tttgtgaatc caagtaagac tgataagctt gtataccaaa atatgctcca
     9001 ccaattattg caccccatgg accccccaat aaagcaccta tcctaccaat catataactg
     9061 attccagtac cagtcatgaa gttagcgaat gtgttagctt gtttattccc atgtattgtg
     9121 ttgacgtaat tccaaacatt aggatcgtat gatctaaaag atatatttag gtcgatttca
     9181 ttcttttgat aagccatata aaatgcccca ttgatataga cgccgtcagc acgtcgttca
     9241 atagtgtcta cacttccatc tggattgaca acctcaagaa cttcatcgct taaaatattt
     9301 acttgcgtat ctccgaaccg cactgatgag ccattctcaa actgagcctc accagataca
     9361 actttagagt ttgccgataa gctatcatca gcaaaaacaa caagcgacgg ggaaatgcta
     9421 gacatacaga aaacagacat aactagcaaa cacatgcatt taaacatctt agacataacg
     9481 gaaactcttt tgtatttttg atttttttca acttttatta tacaataaaa ccaaataaaa
     9541 agaaagcggt aacaatatgc ttaatgcgaa aattttttat atatttttat gtttgatcgt
     9601 tatcgaaact acaggcttgt tgttgttgaa aagaggtctc gaaatgggtt atttagacac
     9661 agaagctatt atcctcgcag ttttttcatt tgctttttac aacctatgtt cattcgcttg
     9721 ggtctgctct acaataaaaa acaataaaaa ataaatagac gtattttcaa aaaaaaaaaa
     9781 atgcatattt atcttagcaa aacgacgatt taaatcgtcg tttttttgta gtacgacggg
     9841 catgtcgtat atctgaggtg taagtcctcg gtgggcacct gctaccggtg aacccaatag
     9901 cgattcccaa gcctgactat cgtgaggtag cagggagagg aagggatagc gaaatcgtgg
     9961 ctctacgaac aggaacgtga tagtaaggcg tatatagcgg ataaggaggc ttcaaactct
    10021 aaagtccaaa aaggtagtcg taacctatat gtgtaaatca cgagagtaat tgaattcgga
    10081 ctaaggtttg tgtgaaaaag ataaatcttt ctagagtcta aagactctgc gtcagatttc
    10141 ctattttcac tgtaaccttt taacgtcctc atatcttgta taaacgagga aagatgtacg
    10201 acttatcccg tgaggtctca tgagtgctga aagcgtagta acaacgaatc atgagaagtc
    10261 agccgaaccc atagtagtga ggaaacttcc gtaatggaag tggagcgaag gggtgaatac
    10321 tcaaacagtc tggggagaga ctgtttgagg tctgtcgcta gaaagagaaa acgacagatt
    10381 gaagtaatcc tacttcactt gtgtctgtaa aatgagtggt ctgatagaac tggactttga
    10441 cacgtactga ctagatgaag gttcaccaat ataagatgtc cctcaggcac caaaacaaga
    10501 aaggaatacg cacatgtcaa aactgctaga taagatatta tcacgcgaaa atatgctgga
    10561 agcctacaat caagtaaaat ccaataaagg ctcagctggg attgatggaa tgactatcga
    10621 agagatggat aattatttca gacaaaactg gcgcttgact aaggaactga taaaacagag
    10681 aaaatataag cctcaaccag ttcttaaagt tgagatacct aaaccagacg gaggcatccg
    10741 tcaactagga attccaacag ttatggatag aatgattcaa caggccattg tccaagtcat
    10801 gagccccatt tgtgaaccct atttctcaga tacgagttat ggtttcagac caaataggtc
    10861 atgcgaaaaa gccatcatga agctcttaga atacttaaat gacggctatg agtggatagt
    10921 ggatatagac ctagagaaat ttttcgatac agttcctcaa gatagattga tgtccttagt
    10981 acataacatt atcgaagacg gagatacgga atccttgatt cgtaagtatc ttcattcagg
    11041 tgttatcatt aatggtcaac gttataaaac actagttggt acaccacagg gaggaaattt
    11101 atctcctctc ttatccaata tcatgcttaa tgaattggac aaggaattag aaaagagggg
    11161 acttcgattt gtgcgctacg cagatgattg tgtgattacg gtcggaagcg aggcagccgc
    11221 taagcgtgtg atgtattcag tcagtcgttt tattgagaaa cggctaggtt tgaaagtaaa
    11281 catgaccaag actaagatta ccagaccaag agagttgaaa tatctaggtt ttgggttctg
    11341 gaaatcatca gatggttgga aaagccgtcc acatcaagat agtgttcgga gatttaagct
    11401 taaattgaag aaactaacac agaggaaatg gagtatagat ctaacaagac gtattgagca
    11461 actgaatttg tctattcgag gatggataaa ctatttctca ttgggaaata tgaaaagtat
    11521 agtcgccagc atagatgagc gcttgcgtac tcgcctacga gtgattatct ggaagcaatg
    11581 gaagaagaaa tcgagacgat tatggggatt tcttaagtta ggagttccta aatggatagc
    11641 agataaggta tctggctggg gcgaccatta tcaattagta gctcagaagt cggtacttaa
    11701 acgtgctata tcaaaaccag tccttgaaaa acgtggactg gtttcgtgtt tggattatta
    11761 ccttgaacga catgcgttaa aagttagttg aaccgccgta tgccgaacgg cacgtacggt
    11821 ggtgtgagag gggctagaga ttatccccta ctcgattaac tcccctgaaa tttattttaa
    11881 ttatgcaaat ttcacgtatt tttgatgctg agacgacgat cctgggaact tttcagatat
    11941 ttttttgact atctaaatct atcattagaa aagcttagag cgccaaagga tttgagcgtt
    12001 tttctgattt ttaagacttt ttccagtctc tttttcgatt gaagatgtaa ttattctact
    12061 aactaactaa cttcttagta ctagccaaca acgataatca taattcctcc taaaattagg
    12121 aataataaag gcaatagttt ttgttttttc atgtaaaaaa cctcactttt gttttctgct
    12181 attttatgct aaaatattaa aaatcaaatt taattccaaa gtttgtaact aaagggggag
    12241 cgctacatgt ctaattcatt tgtcaagttg ttagtctctc aattatttgc aaatttagca
    12301 gatattttct ttagagtaac aatcattgct aacatataca ttatttcaaa atcagtaatt
    12361 gccacatcac tagttcctat cttaatagga atatcctctt ttgttgcgag tcttttagtt
    12421 ccgttggtta ctaaaaggtt agcgctaaat agggttttat ctttatctca atttggaaag
    12481 actatattat tggcgatact ggtaggaatg tttaccgtaa tgcaatccgt agcgcctttg
    12541 gtgacctatc tatttgttgt tgcaatttcc atactagatg gttttgcagc acccgtttcc
    12601 tatgctattg tgccacgcta tgcgaccgat ttgggtaagg ctaattcagc cttatcaatg
    12661 actggtgaag ctgttcaatt gataggttgg ggattaggtg gactcttgtt tgcaacaatt
    12721 ggtctgttac ctaccacgtt tatcatttta gtcttgtata tcatttctag ctttctgatg
    12781 ttatttcttc ctaacgctga agtggaggtg ttagagtcag aaactaatct tgaaattttg
    12841 ctcaaaggtt ggaagttagt tgctagaaat cctagattaa gactttttgt atcagcaaat
    12901 ttattggaaa ttttttcaaa tacgatttgg gtttcttcca ttatacttgt ttttgtaacg
    12961 gagttattaa ataaaacgga aagttactgg ggatattcta atacagcata ctctattggt
    13021 attataatta gtggcttaat tgcttttagg ctatctgaaa agttccttgc tgctaaatgg
    13081 gaaagtattc tttttcctct tgtagctatg gcaatagtga cactgactat tctatatttt
    13141 ccaaacgcac agatgttttt attattttca gctttagttg gtatgttatc gcaactaaaa
    13201 gaagttcctg aaagtgtatt tcttcaggaa acagtagagg aaaatcattt agttaatgtc
    13261 tattccgttc ttgaagtgat ttctacatta gcattttcag tatttgtttt gctaatgagc
    13321 tatattactg agagttttgg tattagcatc agtttttggc tatcagccat ttgtctgatg
    13381 atagaagcta ttttaattta tatcagacga gattatttta aatgagagag tggttaaaaa
    13441 gtctttaaaa atcagaaaag cgcatagtat caggtgttga aaaaacttga tattgtgtgt
    13501 tttattatgg gaagatttac ttcattgtct cctgaaatgg agttttgaag caggatcact
    13561 ctatttgaaa atatacatag aaaaaccgca taatccttaa aataagctga aatctattgg
    13621 aaacatacgg taaacaattg aaaagatact atataaagtg gtacaatggt agaaaaataa
    13681 gctagtaaga atcactctta ttttagcgaa aaattacaga aatgaatggt ttttcttgat
    13741 gaaacagaga aaagaattgt acctctttct tggtcggaca gccttgtatt ttcttatctt
    13801 tctagggctg ctttacttct ttagctatct tggtcagggt caaggaagct ttatctataa
    13861 tgaattttaa cttgaaggag aatccctatt gtgtcaaata aaccaatagc agatatgatt
    13921 gaaaccattg agcattttgc tcagacacag cctagctatc ctgtctataa tgttttgggg
    13981 caggaacaca cttatggcga tttaaaggct gattcggata gtttagctgc agtcattgac
    14041 caactaggct tgcctgagaa gtctcctgtg gttgtttttg gtggccaaga atatgaaatg
    14101 ttggcaacct ttgtagcgct gactaagtca ggtcatgcct acattccaat tgatagccat
    14161 tcggccttgg agcgagtttc agctatttta gaagtagcag agccaagctt gattattgcc
    14221 atttcagcct ttcccttgga gcaggtttct acaccaatga taaatctagc tcaggttcaa
    14281 gaagcctttg cccaagggaa caactatgaa atcacgcatc cagtcaaggg agatgataat
    14341 tactacatta tctttacttc tggtacgact ggtaagccta agggagtgca gatttcacat
    14401 gataatctcc tcagctttac aaactggatg attacggata aggaatttgc gacaccgagt
    14461 cgtccgcaaa tgctggcaca gccaccttat tcttttgact tgtctgtcat gtattgggca
    14521 ccgaccttgg cacttggtgg tacgcttttc actcttcctt cagtcatcac tcaggacttt
    14581 aagcaactct ttgcggctat cttttcattg ccaatcgcta tctggacatc aacaccatcc
    14641 tttgcagata tggccatgtt atctgaatac ttcaacagtg agaaaatgcc tggaatcacg
    14701 catttctact ttgatggtga agaattgacg gtcaaaacag ctcaaaaact gcgcgagcgt
    14761 ttcccaaatg cccgtatcat caatgcttac ggcccaacag aagcgacagt agctctgtca
    14821 gcagttgccg tgacagacga gatgttagcg actctcaaac gcctaccaat cggctatacc
    14881 aaggctgatt ctccaacctt tatcattgac gaggaaggaa ataaactgcc aaatggcgaa
    14941 cagggagaaa tcattgtttc tgggccagct gtttcaaaag gttatatgaa aaatcctgaa
    15001 aaaacagcag aagccttctt tgagtttgaa ggtctgccag cctatcacac aggcgatgtg
    15061 ggaaccatga cagatgaggg cttgcttctc tacggcggac gcatggactt ccagattaag
    15121 tttaacggtt accgcattga gttagaagat gtctctcaaa acctcaacaa gtctcgcttt
    15181 atcgaatctg ctgtcgcagt accgcgctat aacaaggacc acaaggtaca aaatctattg
    15241 gcttatgtca tcttaaaaga cggtgttcgt gagcagtttg agcgagatat cgatattacc
    15301 aaggccatca aggaagacct gacagacatc atgatgtcct atatgatgcc atctaaattc
    15361 ctttaccgag acagtttgcc actaactcca aatggaaaga ttgacatcaa aggattgatt
    15421 aacgaggtga ataagagatg atggagtttt ttcaacagct tcctcattta gagccatatg
    15481 gcaatcctca gtattttgtt tatgtgattg ctgcaacctt gcccatcttt ataggtctct
    15541 ttttcaagaa acgctttgcc tggtatgaag tgttggtaag tctcttcttt attgtcacca
    15601 tgttggtggg tggaaagacc aatcaactag ctgccttggg tatttacctt tgctgggaaa
    15661 tattgctcct gcttttctac aagcattatc gaaaaagcaa ggatggcaag tgggtcttct
    15721 acttagttag ttttctgtcc ctacttccga ttatctttgt caaggtgcaa ccagctatca
    15781 atggaacgca gtctttgctt gggttcttgg gaatttctta cctgaccttt cgttcggttg
    15841 ggattatcat cgagctgaga gatggagtga ttaaggattt taccctctgg gaattcctcc
    15901 gtttccttct cttcatgcca actttctcga gtggtccaat cgatcgcttt aagcgattta
    15961 atgaaaatta tcaggccatt cctgagcgag atgagttgat ggatatgctg gatgaatctg
    16021 tccgctatat catgcggggc tttttgtata agtttatcct agctcatgtt ttaggagaga
    16081 ccttactccc tcctctgaag aatttagcct tgcagtcagg tggcttcttt aatctctatg
    16141 ccttggcagt tatgtatact tttggtctgg agctcttctt tgactttgca ggttattcta
    16201 tgtttgcttt ggccatctca aacttgatgg gaatccgtag ccctatcaac tttaacaagc
    16261 cctttttatc aagggattta aaggagtttt ggaatcgctg gcatatgagt ctgtccttct
    16321 ggttccgtga ctttgtcttt atgcgaatgg tgatggtgtt aaccagaaag aaagtcttta
    16381 aaaatcgtaa tgtaacctca agcatggcct acattgtaaa tatgctgatt atgggatttt
    16441 ggcatggcgt gacctggtac tatatcgcct atggactctt tcatggacta ggcttggtca
    16501 tcaatgatgc ctgggttcgc aagaaaaaaa cgctcaataa ggaacggaaa aaagcgggga
    16561 aggctgccct acctgagaat cgctggattc agtttcttgg catggttgtc actttccatg
    16621 ttgtcatgtt gtcattctta atcttttctg gattcttgaa taatctatgg tttaaaaaat
    16681 aaaaggaaat aaaatatgga tatcaaatca gaagttattg aaattattga tgagttgttt
    16741 atggaagatg tttctgacat gatggatgaa gatctttttg atgcaggtgt cttggatagt
    16801 atgggaacgg ttgagttgat tgtggagatt gagaaccgtt ttgacattcg tgtccctgta
    16861 acagagtttg gtcgtgacga ctggaataca gctaataaaa tcatagctgg tattgtggag
    16921 ctacaaaatg cttaaacgct tatggatgat cttcggaccg gtcttgatcg ctggtttgtt
    16981 ggtttttctg ctcattttct tttatcctac tgagatgcat cataatctag gagctgaaaa
    17041 gcgttcagca gtggctacta ctatcgatag ttttaaggag cgaagtcaaa aagtcagagc
    17101 actatctgat ccaaatgtgc gttttgttcc cttctttggc tctagtgaat ggcttcgttt
    17161 tgacggtgct catcctgcgg tattagctga gaaatacaat cgttcctacc gtccttatct
    17221 tttaggacag gggggagctg catcgcttaa ccaatatttt ggaatgcaac agatgttacc
    17281 acagctggag aataaacaag ttgtgtatgt tatctcacct cagtggttca gtaaaaatgg
    17341 ctatgatcca gcagccttcc agcagtattt taatggagac cagttgacta gttttctgaa
    17401 acatcaatct ggggatcagg ctagtcaata tgcagcgact cgcttactgc aacagttccc
    17461 aaacgtagct atgaaggacc tggttcagaa gttggcaagt aaagaagaat tgtcgacagc
    17521 agacaatgaa atgattgaat tattggctcg ttttaatgaa cgccaagcct ccttttttgg
    17581 tcagttttcg gttagaggct atgttaacta cgataagcat gtagctaagt atttaaaaat
    17641 cttgccagac cagttttctt atcaagcaat agaagatgtt gtcaaagcag atgctgaaaa
    17701 aaatacttcc aataatgaga tgggaatgga aaattatttc tataatgagc agatcaagaa
    17761 ggatttgaag aaattaaagg attctcagaa aagctttacc tatctcaagt cgccagagta
    17821 taatgacttg cagttggttt taacacagtt ttctaaatct aaggtaaacc cgatttttat
    17881 cattccacct gttaataaaa aatggatgga ctatgctggt ctacgagagg atatgtacca
    17941 acaaacggtg cagaagattc gctaccagtt agaaagtcaa ggttttacca atatagcaga
    18001 tttttctaag gacggcgggg agcctttctt tatgaaggac accattcacc ttggttggtt
    18061 gggttggttg gcttttgaca aggcagttga tcctttccta tccaatccca caccagctcc
    18121 gacttaccat ctgaatgagc gctttttcag caaagattgg gcgacttatg atggagatgt
    18181 caaagaattt caatagataa tataatatag aagagttcaa gaatgaaact agttttcacg
    18241 ttttttcttg actctttttt tgcttgtgat ataattaact ggtaaacaaa atgtcaaaga
    18301 atagtatttt tctcttaaaa aagagaagtc taaaaggaaa ggagtcagat atgagacagc
    18361 tagcaaagga tatcaatgct tttttgaatg aggtgatttt gcaggcggaa aatcagcatg
    18421 aaatcctaat aggtcattgc actagcgagg tggccctgac caatactcag gagcatatcc
    18481 ttatgctctt gtcagaggaa tctttaacaa attcagaatt ggcccgtcgt ctcaatgtca
    18541 gtcaggcggc agttaccaag gccattaagt ctttggtcaa ggaagggatg ttggaaacat
    18601 ctaaagattc taaagatgcg cgtgtgattt tttatcagtt gactgacttg gctcgtccaa
    18661 tcgctgagga gcaccaccat caccatgagc atacactttt aacctatgaa caagtggcga
    18721 ctcagtttac tccaaatgaa caaaaagtga ttcagcggtt tttgactgct ttagtaggag
    18781 aaatcaaata atgagatata ttacggtaga ggatttgtcc ttctattatg ataaggagcc
    18841 tgttcttgaa catatcaatt atagtgttga tagtggggaa tttgttacct tgactgggga
    18901 aaatggagcg gctaagacga cgctcatcaa ggctagtctt ggaattctgc aaccacgcat
    18961 tggaaaggtg gctatttcaa agacaaatac gcaaggtaag aaattgagaa tagcctatct
    19021 tcctcaacaa attgccagtt ttaatgctgg ttttccaagt acggtctatg aatttgtcaa
    19081 gtcgggtcgc tatccgagaa aaggctggtt ccgtcgtttg aatgctcatg atgaggagca
    19141 tatcaaggcc agtctggact cagttggcat gtgggaacat cgagacaaac gcttggggtc
    19201 tctatctggg ggacaaaagc agcgagcggt gattgcgcgt atgtttgctt ctgaccctga
    19261 tgtgtttatc ctagacgagc cgacaacggg gatggatgca ggaagtaaaa atgaatttta
    19321 cgaactcatg caccacaacg cccatcatca tggcaaggct gttttgatga ttacccatga
    19381 ccctgaagaa gttaaggatt atgcggatcg caatattcat ctagtccgta accaagactc
    19441 gccatggcgt tgtttcaatg ttcatgagaa tggccaggag gtgggccatg cttagtttat
    19501 tatcttacga ctttatacaa cgcgcctttt tggcggttat tgctatgagt cttttctcac
    19561 cggtattggg aaccttcctc atcttgcgtc gtcagagttt gatgagtgat acccttagcc
    19621 acgtctcact ttcaggtgta gcctttggtc tggttttggg gatttctcca actgtttcta
    19681 ctattgccat tgtcttgatt gcggcggtct ttctggagta tctccgtacg gtttacaaga
    19741 gctttatgga aatcgggaca gctatcctca tgtcaacagg tctggctgtt tctctgattg
    19801 tcatgagcaa gggtaaaagc tcgagttcaa tgagtttgga ccaatatctc tttggttcga
    19861 tcgtgactat cagtgaagaa caggtcattt ccctctttgt cattgcggcg gttgttttga
    19921 ttttgacctt tctctttctt cgtcctatgt atatcttaac ttttgacgaa gatacggcct
    19981 ttgtggatgg cttgccagtt cgtaccatgt ccattctttt taacatggtg acaggggtgg
    20041 ctattgccct tatgattcct gcagcaggag ctcttctggt atcgaccatt atggtcttgc
    20101 cagctagtat tgccctgcgt ctggggaaaa actttaaatc ggttatgctg cttgccagtg
    20161 cgattggctt tttgggaatg gtagcaggac tttacatttc ctactatgca gaaacacctg
    20221 caagtgcaag tattaccatt atttttgtaa ctgtctttat actaatcagt ttagtaagac
    20281 gttttatcaa ataggagacg aacgtgaaaa aaattagctt attactagcc agtctatgtg
    20341 ccttgttttt agtggcttgt tccaatcaaa aacaggcaga tggtaaactc aatatcgtga
    20401 caacctttta ccctgtctat gaatttacca agcaagtcgc aggagatacg gctaatgtag
    20461 aactcctaat cggtgctggg acagaacctc atgaatacga accatctgcc aaggcagttg
    20521 ccaaaatcca agatgcagat accttcgttt atgaaaatga aaacatggaa acatgggtac
    20581 ctaaattgct agataccttg gataagaaaa aagtgaaaac catcaaggcg acaggcgata
    20641 tgttgctctt gccaggtggc gaggaagaag agggagacca tgaccatgga gaagaaggtc
    20701 atcaccatga gtttgacccc catgtttggt tatcaccagt tcgtgccatt aaactagtag
    20761 agcacatccg cgacagcttg tcagcagatt atcctgataa aaaagagacc tttgagaaga
    20821 atgcagctgc ctatatcgaa aaattgcaag ccttggataa ggcttacgca gaaggcttgt
    20881 ctcaagcaaa acaaaagagc tttgtgactc aacacgcagc ctttaactat cttgccttgg
    20941 actatggact caaacaagtc gcaatctcag gactttctcc agatgcagag ccatcagctg
    21001 ctcgcttggc agaattgaca gagtatgtca agaaaaataa aatcgcctat atctattttg
    21061 aagaaaatgc ctcacaagcc cttgctaaca cactttcaaa agaagcaggt gtcaaaactg
    21121 atgtcctcaa tcctttagaa agtctgacag aagaggacac caaggctgga gaaaactaca
    21181 tttccgtgat ggagaaaaac ctcaaggctt tgaaacaaac aacagaccaa gaaggcccag
    21241 caatcgaacc tgaaaaggca gaggatacca agacagtcca aaatggttac ttcgaggatg
    21301 cagctgtcaa ggaccgcacc ttgagtgact atgcaggtaa ctggcaatca gtttatcctt
    21361 tccttgaaga cggcacgttt gaccaagtct ttgactacaa ggctaagttg actggtaaga
    21421 tgacccaggc tgagtacaag gcttactata caaaaggcta tcagacagat gtgactaaga
    21481 ttaacattac tgataatact atggaatttg ttcaaggtgg acaaagcaag aaatacactt
    21541 acaagtatgt cggtaagaaa attttgactt acaagaaagg caatcgtggc gtgcgtttcc
    21601 tctttgaagc cacagatgct gacgctggac aattcaaata tgttcagttt agtgaccaca
    21661 atgttgcccc agttaaggca gaacatttcc atatcttttt tggaggtaca agccaagaag
    21721 ctctatttga agaaatggat aattggccaa cctactaccc agataaccta tctggccaag
    21781 aaatcgccca agaaatgttg gcgcattgat gagaaatcga ctagttcata agagctagtc
    21841 ggttttttga tactagatgg ctaacttcat tatagacctt tcagaactaa gggaacctga
    21901 aaaaataaat aaaactcttg aaatatctgg acttatatgc tagaatgtag ttagtgtata
    21961 aggttcatta gaacaccaaa gcccctaact agtgccatag ttaagggctt ttttgacgta
    22021 tcttagcgat ttaatgggtg tttataggct agtcagtcgg tctacttctt accatctagc
    22081 catttgcaga cgaaatacaa gataattcca gctataacat ctgctataat agtaagagca
    22141 agctctggta taaggctcat tagtttcacc tcctctcacg aacccatagg aacgtaatcg
    22201 gtaaccgatg acaaaaatag tataccacac taaatttaga tcatcaacct cacttaatac
    22261 ttgaactatt gtagattata tgctaaaatg tagttaattg ataaggttca ttagaacacc
    22321 aaagccccta actatggccg tagttaaggg cttttttgac gtatcttagc gatttaacgg
    22381 gtgtttatag gctagtcact cggtctactc cttactatct agccatctgc agacgaaata
    22441 caagataatt ccagctataa catccgctat aatagtaaga gcgagctcta tgataaggct
    22501 cattagtttc acctcctctc acgaacccat aggaacgtaa tcggtaaccg atgacaaaaa
    22561 tagtatacca caatacattt agatcatcaa ggtcacttaa ttcttgaaat atagggtcta
    22621 ggcgacaaag tctttaaaat cagaaaaacg cataatatca ggtgtgcaaa aacttgatac
    22681 tatgcgtttt attgtgggaa ggtttactcc attttctcct gaaattgagt ttttgtccag
    22741 cctctgtttt tagaattgct aagaaaataa tgtcatgtgg tgaatatttg taaatcagtc
    22801 agcagacaga acgaatactc ttcgaaaatc tcttcacatc atgtcagctt cgtcttgccg
    22861 tgtccatggg ggacttgact tcatcagttt tcaaatctgt actttgagca agctgagact
    22921 agcttcctat ttgattttca ttgaatatca gaaacccatt ctccatcaaa taattcgact
    22981 gcatctaata atttttggtc tggcaaggtg tcagaaataa aggttgtgta tttggatagg
    23041 ggattaattt taaaaaaatc cagtcttgta aaatttagaa ctatcaatca gtaagatggt
    23101 ttcatgggct ttgtcaataa tattcttttt tgaaatagct tggctgagag aagcttcata
    23161 aacatattgg tcatcaatac ctcttgctga acaaaatgct aaatcgatat taaaatgatc
    23221 taataaagaa ttttccttat catagttgac cacggaacag gattgatgtt tgacctcgcc
    23281 agatgtgata aagattttgg agctatcttt aacagtttca gatagggttt gtgcagtatg
    23341 taaaccattt gtaaaaataa tcaaattatc aagttcagaa agatagggac agagttcgta
    23401 gacagtcgta ctggaatcaa gataaataca catgcctgac cgaataaagt ctttagcgag
    23461 actagcgatt agtctttttt gcctagtact ttctccttcc cgtatttgat gagaaagttc
    23521 aattgtgttc atagaggaca gggtcacgta tccgtgcttt cttttgataa gaccttgatt
    23581 ttctaagaaa attaaatcac gacgtaaggt acttgtgctg gagaaagtga tttctgccag
    23641 ctcttttacg gcaattcttt ttttcttttt aataatttca atcaattcaa gtacacgttc
    23701 atcttttatc ataagctcct cctaatttat catttcaact atattatagc acaaattgga
    23761 ggaatttgaa ttatttttat gaatattggg ttaatatttg aacattattc aagtaagcgt
    23821 tcacatattg aaaaaataaa acgtggggat tataataaag ttaatcaagg acgaagagag
    23881 aagaaaaatg gaagcggttt tagcaataga tttaggtgcg acttctggaa gagcaatcgt
    23941 tggttacctt tctgaaaata aactagtaat ggaagaaata aatcgctttt ctaatctacc
    24001 tattagagta aaagggcatt tatcttggga tattgacttt ctactagcta aaattcttga
    24061 aagtatccgc ttggctaata ctagttacaa gattttatct atcggtattg acacatgggg
    24121 agttgatttt ggactgattg ataatgaagg taagctgtta ttacaacctg ttcattatcg
    24181 tgatgaaaga acaaagggag tgttaaagga aatatctgaa atgactgaat tagaaaaact
    24241 gtattcagag acaggaaatc agattatgga gataaatacc ttgtttcaac tctttaaggc
    24301 acgtcaagaa tctcctgact ctttctataa gaccaataag attcttttaa tgccagattt
    24361 gtttaattat ctcttgacag gtaagtttgc tacagaaaaa agcattgctt caacaactca
    24421 attatttgat cctaggagtc aaaactggaa tcagaatatc ttaaaactat ttgaattgga
    24481 ttcatcttta cttcctgaga ttgtttcaga gggaaatgtt cttggaagga taaaaaagga
    24541 gtatggttta ggcgatattc ctgttgtgaa tgtttgtagt catgatacag caagcgcgat
    24601 tgtctcagta cctaagacag aaggtagttt atttatttca tcaggtactt ggtctttggt
    24661 tggagtggaa cttacttcac cgcttcttac taccgaatcc ttcagttatg gatttacaaa
    24721 tgaagtcggt aaagatggag tgattacatt tctgaagaat tgtacagggt tgtggatcat
    24781 agaggaacta agacgttcat ttgaacgaag agggaaagcc tattcttttg atgatattag
    24841 gacaatggtg gagaaagaaa aagaaaatct tcctctgatt gatactgaat caactgaatt
    24901 tgcaacagaa tctgatatgc acaagacttt gacagaatat ctagcttatc atcatgaaac
    24961 tagagagtgg acagatggac aactatttaa gattgtttat gaaagcctag ctgaaacgta
    25021 taggaaagcg atagagttac tagaagaact aactcataag gtttataaga ggatatatgt
    25081 gattggagga ggtgctagag ccagttactt taaccaaatg attgctgata gaactggtaa
    25141 agaggttctt acaggtttga ctgagggtac agctgtgggg aatattgttg tgcagctatt
    25201 gagtcaaggt aaaataaacg aggatacaga gttaaaggat attatgacaa atattgctga
    25261 gacacaatat tattatcctc agctatcata aacaaaaata aaaaaacgga ggttttgtca
    25321 tgaacaaaaa aagtcttttg aaatgcgctg ttatcgggtt agtagctacc tttggtttag
    25381 cagcttgtgg aacatctaag gatgcaagcg gtggaagttc ttctggtaaa gaagtattag
    25441 aattttatca tgggtatcat catagtgaag atgaatggcc tgtagcgaag actatgcgag
    25501 atttatatga taaatttgca gaagaacata aagatagtgg tgtagaattt aaaccaactc
    25561 ctgtaaatgg tgatttgaag gatataatga ataacaaggt tgctagtggt gaatttcctg
    25621 atgtgattga cctagcaggt aatgcagtat cattagttgc gattgaacaa aaacttgttt
    25681 tagatttgaa accatatatc gattcaaata agcttgaaaa aaatgttggt ttgaactata
    25741 agcaaaacca aaaagatgga aaaatttata cagttcacga acagttattt acaatgggtt
    25801 tgtggtacaa caaggacata tttgcaaaag cgggtgctaa gacacctgat cagtggaata
    25861 cttgggatga tttcactcag gcaatggcaa gcattagaaa acaagatgga gtgtatgcat
    25921 ttggtgctgg agaaccatct attcgcttgt ttaatacagt attaggaacc acagaaaatg
    25981 gacgtaaatt attagataaa ccattaacaa aagaaggaat tgaatctaaa gaatttgcag
    26041 atgctttgaa aatggttatg aaagaaattc aagctaatgg ttctaagaat gctggtggcg
    26101 atgccaatgc ttattccaaa gatttccaag aaggtaaatc tgcagtattc tttaatggtg
    26161 tgtgggcttc tggtgagatg tctaagaacc caagtcttgc acctggaatc tatcctgcag
    26221 gtgtagcaat tagttcttct ggaggtggta tcactatctc aagtaagatg tctgaagcta
    26281 aacaaaaact agctcttgag tttttgaaat atatgactag cgatgatgta caaaaagtaa
    26341 tttttgaaaa agtaggagca aatccttcta atgaaaatgt aaatgtaaaa gaactttcag
    26401 aaaagagttc agaagctact actaaaattt taggacaagc aatcactcaa gttaagaatg
    26461 caaaagcagt tgttccaact gtaagtgatg tttggggtgg cgatgtacag acagctatta
    26521 tcaatgcatt gacagaaagt gctgctgaaa atgtagatgt tgatcaaaaa gtaaaatcta
    26581 ctcaagatgt gttgaaatca ttgattggtt aattgtttta gggagtgaaa acgaagctca
    26641 gttggcactg ccaactgagc ttgattttac ttatatagtt gaaaaaagga gtttgtttat
    26701 ggcacaaaaa attatgagcc tacaaaatag gaagaatcaa aaaagacgat ttatcttttt
    26761 atttcttttg ccaacgttga tttgtttctt tctattttat ttttattctg tagtaacaat
//
LOCUS       DAVMAF010000020        26474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMAF010000020 DAVMAF010000000
VERSION     DAVMAF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.020"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(136..903)
                     /locus_tag="VYO97_000869"
     CDS             complement(136..903)
                     /locus_tag="VYO97_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1753690.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKHAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGSRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(900..1766)
                     /locus_tag="VYO97_000870"
     CDS             complement(900..1766)
                     /locus_tag="VYO97_000870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1753691.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VYO97_000871"
     CDS             complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VYO97_000871"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1753692.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VYO97_000872"
     CDS             complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VYO97_000872"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1753693.1"
                     /translation="MIDIQEIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKCRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(3601..4086)
                     /locus_tag="VYO97_000873"
     CDS             complement(3601..4086)
                     /locus_tag="VYO97_000873"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1753694.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VYO97_000874"
     CDS             complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VYO97_000874"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1753695.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VYO97_000875"
     CDS             complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VYO97_000875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1753696.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VYO97_000876"
     CDS             complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VYO97_000876"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1753697.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VYO97_000877"
     CDS             complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VYO97_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1753698.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(8117..8341)
                     /locus_tag="VYO97_000878"
     CDS             complement(8117..8341)
                     /locus_tag="VYO97_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1753699.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8401..9375)
                     /locus_tag="VYO97_000879"
     CDS             complement(8401..9375)
                     /locus_tag="VYO97_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1753700.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSIAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9375..9809)
                     /locus_tag="VYO97_000880"
     CDS             complement(9375..9809)
                     /locus_tag="VYO97_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1753701.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9903..10688)
                     /locus_tag="VYO97_000881"
     CDS             complement(9903..10688)
                     /locus_tag="VYO97_000881"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1753702.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11013..12377
                     /locus_tag="VYO97_000882"
     CDS             11013..12377
                     /locus_tag="VYO97_000882"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1753703.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12380..12751
                     /locus_tag="VYO97_000883"
     CDS             12380..12751
                     /locus_tag="VYO97_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1753704.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            12965..14239
                     /gene="serS"
                     /locus_tag="VYO97_000884"
     CDS             12965..14239
                     /gene="serS"
                     /locus_tag="VYO97_000884"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1753705.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDASVTIPEALRPYMGGAEVIKP"
     gene            complement(14264..15163)
                     /locus_tag="VYO97_000885"
     CDS             complement(14264..15163)
                     /locus_tag="VYO97_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1753706.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(15273..15821)
                     /locus_tag="VYO97_000886"
     CDS             complement(15273..15821)
                     /locus_tag="VYO97_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1753707.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16039..17361)
                     /locus_tag="VYO97_000887"
     CDS             complement(16039..17361)
                     /locus_tag="VYO97_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1753708.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(17452..17540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17704..20040)
                     /locus_tag="VYO97_000888"
     CDS             complement(17704..20040)
                     /locus_tag="VYO97_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1753709.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLTSREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKEIELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20144..20692)
                     /locus_tag="VYO97_000889"
     CDS             complement(20144..20692)
                     /locus_tag="VYO97_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1753710.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20689..20991)
                     /gene="zapA"
                     /locus_tag="VYO97_000890"
     CDS             complement(20689..20991)
                     /gene="zapA"
                     /locus_tag="VYO97_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1753711.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21078..21998
                     /locus_tag="VYO97_000891"
     CDS             21078..21998
                     /locus_tag="VYO97_000891"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1753712.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISNPISNPI
                     SNPISNPISLEEKAEGKYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKV
                     ASQILQAYGMQGLNFCAKLHFKNTEKAKNA"
     gene            22010..22624
                     /gene="lepB"
                     /locus_tag="VYO97_000892"
     CDS             22010..22624
                     /gene="lepB"
                     /locus_tag="VYO97_000892"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1753713.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22752..25118
                     /locus_tag="VYO97_000893"
     CDS             22752..25118
                     /locus_tag="VYO97_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1753714.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(25166..26449)
                     /locus_tag="VYO97_000894"
     CDS             complement(25166..26449)
                     /locus_tag="VYO97_000894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1753715.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         7605 a         6154 c         5163 g         7552 t
ORIGIN      
        1 atttttgtaa gcttacatct acagtataca tcttttttgt ctattttata gtctatctcc
       61 tcaactgtca atttttcaac ctgaattgca tttttccata aaaaatgaga cctttctagt
      121 ctcatttagt cattcttagt attttctaaa tcgttgatag cgttcttcca gcaactcttc
      181 tagcggtttt tgtgaaagtc tagccagctc cgtttggagt tcttttttga cactcttaat
      241 cagttcttta ctagaaagtc ctacttcaga aatcacctta tccaccacgt ccatttctag
      301 cagctcatgc gaagtgattt tcatcaattc tgccgcttcc atggcacgac tgccatcctt
      361 ccatagaatg gaagcaaagc cttctggact tagaatggca taaatagaat tttccagcat
      421 ccagacacgg tccgcgactg ctagagccag agccccacct gaaccacctt caccgataat
      481 gatagcaata atcggaactt tcaggtcact catttccatg agattgcgag cgatagcttc
      541 cccttgacca cgttcttccg ctccgacacc aggataagca cctgctgtat tgataaaggt
      601 cacaactgga cggccaaatt tctcagcatg tttcatcaac cgcagtgcct ttcggtagcc
      661 ttctggatgt ggttggccaa aattccgttt gaggttgtct tgcaaactct tgcctttttg
      721 gataccaacc actgttacag cttgatctcc aagccaacca ataccaccaa caactgcacc
      781 atcatcacga aaagaacggt caccatgtaa ttggataaat tcatcaaaaa tgcctgtcgc
      841 aaagtccaag gttgtcaagc gactctgctc acgcgcttct ctgactattt ttgcaatatt
      901 catctaggac tccctccatg caatctgact aggctagcaa tcgtatctgg taagtctctt
      961 cttttgacaa tagcatccac aaagccatgt tctaatagga attctgcctt ttggaaatcc
     1021 tcaggcaagc tttcacgaac cgtattttca atcacacgac gcccagcaaa accaaccaag
     1081 ctctgtggtt cagccagaat gatatcgcct tccatagcga aagaagctgt cacaccacca
     1141 gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag catttgaatg acgtttaacc
     1201 gccgcagaga tcttagccat ctgcatgaga ctcatgattc cttcctgcat acgggctcca
     1261 ccagaggctg tgaataggac aactggcaat ttttcgacag tcgcatactc aaacaaacga
     1321 gtgatttttt cacctacaac cgtacccata gaagccatga taaagttaga atccataatc
     1381 ccgagagcca cagtctgacc tttaataaga gcagttcctg tcacaacggc ttcatgcaga
     1441 cctgtttttt cacgcataga tgccagtttc ttttggtaac cagggaaatg caagggatcc
     1501 ttgctttcaa tccctgtaaa caattctttg aaggttccca tatcaatcgt caaagccaag
     1561 cgttcttggg cagaaatacg aaaggtatag ctacagtgcg gacagatacg ttcacttccc
     1621 agatccttct gatagatggt atgcttacag cctggacact gggaaaataa ttcatctgga
     1681 acctctggct tagcttgagg tttttcccta accgaacgat tgggattgat tcgaatatac
     1741 ttatcttttt tactaaatag agccattgat tccccttttc ggtttaaact cttaaagtca
     1801 ttttattctt tttcttgata tttaggtaag aaggtttcca tcaagaagga agtatcataa
     1861 tccccagcaa tgacattgcg atctgaaatg aggtcaagct ggaaatctgc attggtctgc
     1921 actccttcaa tctctaattc atagagggca cgttgcattt tcatcaaggc gtcaaaacga
     1981 ttttcgccgt gtactatgat tttggcaatc atactatcat aataaggcgg aatggtataa
     2041 cctggataaa ctgctgaatc cacgcgcaag ccaactccac cacttggcag atagagatta
     2101 gtaatcttac ctggacttgg agcaaagtta aaggctgggt tttctgcatt gatacgacac
     2161 tcgatggcat gaccgcgtag gacaatatct tcttgcttaa cagacaaagg ctgacctgcc
     2221 gcaatgcaaa tctgttcctt aacgatatca acacctgaaa caaactctgt tactggatgt
     2281 tctacctgaa cacgagtatt catctccatg aaatagaaat tgctacttgc ttcatcaaga
     2341 agaaattcaa tggttcctgc attctcatag ccaacaaact ctgccgctcg aacagcagca
     2401 gcacctattt catgacgcag cgtttttccg attgcaatcg agggactttc ttccaaaacc
     2461 ttttggttat tcctttgaag agaacaatcc cgctcaccca agtgaatcac atgtccatgc
     2521 tcatcaccta ggatttgaac ctcaatgtgc cgagctggat agataacccg ttctatgtac
     2581 atggcaccat tgccataatt ggccttggcc tcactagagg cagtttcaaa ggcagaaacg
     2641 aggtcatctg gtttttcaac cttacgaatc cctttaccac ctccacctgc tgaagccttg
     2701 agcataacag gatagccaat tttttcagca acaatcaaag cttcttcaga gttatgcact
     2761 tctccatctg aacctggtat aacaggcaca cctgctttaa tcatctgagc acgcgcattg
     2821 atcttatccc ccatcatatc cataacatga ccagatggac cgataaactt gatacctact
     2881 tcttcacaca tggtcgcaaa tttggaattt tcactgagaa atccaaaacc agggtgaata
     2941 gcttctgcct cagtcaagac tgcagctgat agaactgcat taatattgag ataagactct
     3001 gttgccttgc caggaccaat acaaactgct tcatctgcca aaagcgtatg aagagcttcc
     3061 ttatcagcag ttgaataaac cgctaccgtc gcaatcccca attcacgtgc cgcacggata
     3121 atacgaaccg caatttcacc acgattggca attaaaattt ttcgaaacat ggagaacctc
     3181 cttagttccc aattgcaaaa gtaaggatac cactggctgc aagcttgcca tccacttcag
     3241 cctttgcttc aaccacagct atggtgccac gacattttac aaaagtcgct gtcataacca
     3301 attggtcgcc tggtacaact tgcttcttga acttaacctt gtccatacca gcgtaaaaga
     3361 ccagttttcc tttattttca ggttttgata actccaacac accagcagtt tgcgccaagg
     3421 cttccataat cagaacacct ggcataactg ggtattgagg aaagtggccg ttaaagaaag
     3481 gttcgttaat ggtcacattt ttgatagcaa caatggtatc ctcgctcact tccaagacac
     3541 ggtccactag aagcatagga taacggtggg gaagagcttc tttgatttct tgaatatcga
     3601 tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt tcttcgttag agacgagaat
     3661 ttccgttacc acaccatcct taggagctgg gatttcattc atgactttca tggcttcgat
     3721 aattaccaat gtttgacctt ttttgacact atcaccaact gtaacgaagg caggtttatc
     3781 tggtccagca gccaagtaaa ccactccaac aagtggactc tctacaagat ttccctcagt
     3841 agccacactt gcttcagctg gagctggaac ttcttctgct acagtctctg ctggagcaga
     3901 tgtaggagct actggactcg gtgttgctag aacgggtgct ggagcgactt gagttgcaac
     3961 ttcaggcaca ggtctcgctt cattcttgct aaactgcaac tcatccgtcc catttttata
     4021 agaaaattct ctcaaacttg actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa
     4081 attcatactt atctattctc ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa
     4141 ccaaaagtat ttgaaatagc gtatggaatt tctttctcca agccttgtcc ataaacgaca
     4201 ttagcttcga tataatctga tacttcactt gtcccagctg tcattggtac aaagttatgg
     4261 cgcatagctt cgatggtgac gatagcttct actgcacccg cagcccccag caaatgtcct
     4321 gtaaaagact tggttgatga tacaggtact tccttaccaa gaacagctac gatagctcca
     4381 ctttctcctt tttcattggc aggagttgac gttccgtgag cattgacata ggctacttgc
     4441 tctggagaaa tctcagcttc ttccaaggct agtttgatgg ccttgatagc tccctgacct
     4501 tctggatgtg gagaagtcat gtggtaggca tcacaagtat ttccgtaacc aaccacttca
     4561 gccaggatag tagctccacg tttttcagcg tgttcaagac tttctaaaac caacatccct
     4621 gaaccttcac ccataacaaa cccattgcga tccttatcaa atgggatcga agcacgagtt
     4681 ggatcctctg tagtagagag agctgttaag gcttggaaac cagcgatggc aaaaggtgtg
     4741 atagaagctt ctgttcctcc caccaacatc acatcttgga aaccaaactt aatggagcgg
     4801 aaggcatccc caatcgcatc atttgatgaa gagcaggcag tattgataga tttacaaaca
     4861 ccgtttgcac caaaacgcat ggctacattc ccagaagcca tatttggtaa agcttttgga
     4921 agagtcattg gtttgacacg tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca
     4981 atttccttga ttccaccaat accagatgca acgataacac caaaacgatc cctattaaga
     5041 gcctctacat caagattggc atgatttaca gcctcttggg ctgcatacaa ggcatataaa
     5101 gaatagttat caaaacggtt ggtatctttt tttacaaagt atttatcgaa cggaaaatct
     5161 tggatttctg ccgcattatg cacatcaaag tcactatgat caaattttgt aatgccacca
     5221 atgccgattt tcccagttgc taaactattc caaaattctt ctggtgtatt tccgattgga
     5281 gatgttactc cataacctgt taccactact cgatttagtt tcattctttt cacctctagc
     5341 tttcgctaca tacttaagcc accatcaatg gcaatcactt gaccagttag ataatcttgg
     5401 cctgctaaaa atactgtcaa atctgcaacc tgctctgcct gcccaaattc tttcatcgga
     5461 atctgagcta gtgtagcttc cttaatctta tctgataaga tagctgtcat atcagactca
     5521 atcattcctg gagcaatcac attgactcgt atattccgac tagcgacctc gcgtgccaca
     5581 gacttggtaa agccaatcaa gccagcctta gaagcagcat agttagcttg accaatattc
     5641 cccatcaaac caacaacact agacatatta atgatagcac cttctctggc tttcatcatc
     5701 ggtttcaaga ctgattgtgt catattaaag gcaccagtca gattgacctt gagcactttt
     5761 tcaaaatctg cttctgtcat cttgagcata agagtatctt gggtaatccc tgcattgttg
     5821 accaaaacat ctactgaacc cagttctgca atagcttgat caatcatacg cttagcgtct
     5881 gcaaaatctg atacatctcc tgaaatggga accaccttga taccatagtt tgaaaactca
     5941 gcgagcaatt cttctgagat tgccccacga ctgtttaaga caatgttggc tcctgcttga
     6001 gcaaacttgt gggcgatggc aagaccaatt ccacgactcg aacctgtaat aaagatattt
     6061 ttatgttcta gtttcatttt tttcctttca aaacttctac ttattttagt ctatttttct
     6121 aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta agtgagcagt ttgatcaatt
     6181 tttttaacaa aacctgacaa gactttcccc ggtccaatct cgataaagtt gcttatgcct
     6241 gcttcttgca tgaccccaat actttcatag aaacgaacgg gttccttgac ctgacgcgtc
     6301 aagagctgag caatgtcctc tttttgcatc acagcagctt ctgtattgcc gactagggga
     6361 caagtaaaat ctgaaaaact tacctgagct agagtttcag ctagtttctg gctagctggc
     6421 tcaaggagag cggtgtgaaa gggacctgac accttaagag gaatcaagcg tttggcacct
     6481 gcttcttgca aaagttcaac cgctcgatca actgcaacca cttctccagc aatgacgatt
     6541 tgtgcaggtg tgttatagtt ggctggagta accactccaa gttcagaagc tttttgacag
     6601 gcttcttcaa tgacctctac tggcgtattg agaactgcta ccatcttgcc agagtcagca
     6661 ggagccgctt cttccatata ggctccacgc ttagctacca aggcaaccgc atcttcaaaa
     6721 tccaaggcgc cgcttgccac caaggcagag tattctccaa gagacaaacc agcaaccata
     6781 tcaggctgat agcccttttc ttgcaataaa cggtagatag caaccgaagt cgctagaatg
     6841 gctggttgcg tatagcgggt ctgattgagt ttgtcttctt ccgtatcgat gagataacgc
     6901 aaatcataac ctagcacctg actcgctcga tcaatcgttt ctttaacaat cggatactga
     6961 tcatagaaat cccgtcccat ccctagatac tgggcacctt gaccagcaaa taaaaaggct
     7021 gttttagtca tttcttacaa ctcctgtcca gcgagaggct tcttcttgaa ttttcttagc
     7081 ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt tcttctttag aaacaagccc
     7141 tgcgatttga cctgccatga cagagccacc ctccacatca ccgtgaacaa ctgctttggc
     7201 tagggcaccg gctcccattt gttcaaagat ttctaaatca ggatcttcct gcttaaaggc
     7261 atctttttca gccagttcaa aatctctagt caactgattt ttaatagcac gaacagcatg
     7321 accaaagtgc tgagctgaaa tcgtagtgtc aatatccctt gcttttaaaa ttttctcctt
     7381 gtagtttgga tgggcattcg actcttttgc aactacaaac cgtgtcccca cctgtacagc
     7441 ctctgcacct agcataaagc cagccgcagc accttcacca tccgcaattc ctcctgcagc
     7501 aataacagga atagatacag ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt
     7561 accgatatgc cccccagctt ccattccttc tgcaataaca gcgtctgcac cgattttttc
     7621 catgcgttta gctaaagcga cactaggaac gacaggaata acgattatcc cagcttcatg
     7681 gaaacgttcc atatacttgc ttggatttcc tgctcctgtt gtgacaactt taacaccttc
     7741 ttcaataacg agatccacga tatcttccac aaagggagat aagagcatga tgttgacccc
     7801 aaagggttta tcagtcaatg atttgatttt atcaatattg gccttgacaa cttctttcgg
     7861 ggcatttccc ccaccgataa ttcctaatcc tccagccttg gaaacagccc ctgccaaatc
     7921 accatcagca acccaggcca tccctccttg gaaaatagga tagtcaatct tcaataattc
     7981 tgtaatacgc gttttcatag tgcctccaac cttccttgct tacgtaatag ttcgatttca
     8041 ccataatttg acagtcaaac tattacctaa acaagaggga gtgggtttct ccctactcct
     8101 tctactaata ttctgcttat tttgcttgct cttcaacgta agcaaccaag tcaccaactg
     8161 ttttcaagtc attttctgct tcgatttgga tatcaaaagc atcttcgatt tctgagatta
     8221 cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa agttgattca agtgttactt
     8281 ctgatgcgtc ttttccaagt tcttcaacga taatttcttg tactttttca aatactgcca
     8341 tgataggact cctttaaaat aaatagtttt tttataacaa tgtgttcacc acatgattac
     8401 ctaaattgta agaatgagcg tgccccaggt caagcctcca ccgaagcctg atagaagaac
     8461 agtctggcta ccatctaaag ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg
     8521 gatactggct gcactggtat tgccatattc catcatattg gctggaagtt tggctcggtc
     8581 aacaccaatt tttctagcca tcttatccaa aatacggtca ttggcttgat gaagtagcag
     8641 ataatccaag tctgtcacct ctataggaga ttcatcaata gtctgcttga tagacttggc
     8701 tacatctcga atggcaaaat caaagactgt gcgtccatcc atcttcaaaa atgaatctgc
     8761 actttcttga tctgaaaatg gagaatgcaa acctgaatgc ccataagtta aacactcgct
     8821 gcgacttcca tcgctattga gactctcagc taagaaatgc tcttgctcgc tagcttctaa
     8881 caagacacca ccagcaccat ctccaaacaa cacagctatt gatcgatccg accaatcgac
     8941 tgccttagag agggtttcac taccaatcac caagcctttt tgaaagcgac cagaagcgat
     9001 aaacttttca gcagttgaaa gagcaaatac aaatccactg caagccgcgg ttaagtcaaa
     9061 agcaaaggct ttattagcac caatattagc ttgaacacga gcagctgtag agggcatcat
     9121 cgaatctgga gtaatggtag ctaggatgat aaaatccagt tcttctcctg ttattccagc
     9181 ttttgccatc agtttcttag caacctctgt agccaaatca ctggtagatt ctgttcttga
     9241 aatatgcctt tgtcgtattc ccgttcgact tgaaatccac tcatcattgg tatccataat
     9301 ctgagccaag tcgtgatttg taaccacttg ctctggcaca taatgagcaa cctgacttat
     9361 ttttgcaaaa gccattattt caaatcctcc aaaaattggt aaagattagt caagccttta
     9421 cccatgacag caatttcttc ctcgctcatg ccatcaataa ttttttctac catggccttg
     9481 tggaagcgtt tatgcagtct atgaatcaag cgacccttct ttgtcaaatg cagatgcacc
     9541 acacgacgat cctgttctga ccgaactcgc tcaatgtagc ccttacgctc taaattgttc
     9601 aaacttgtcg taacagttcc aagagttacc atcaactctt ttgacacttg acttggagtc
     9661 acatctggag ccttaccgat gacatcaatc gtatgcattt ctttgatgga gatatcctta
     9721 aaacgactac ctctcaagtt cacttcctca attacaagga cattgttaaa tatagatgtt
     9781 aaatattcat tgattcgttg gtagtccatt tttcatatcc ctccttctta aaaaaacttt
     9841 tacaatcaaa acatttgaca gaaagaatta tatcaaaatt caaagaatga tgcaagtatt
     9901 ttttattttc ctgtaaattt aggtcttctt ctctccgaat gagctcgcac tccttctttg
     9961 aaatcctctg tttgagctaa ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc
    10021 cagtctttaa attggctctc ccaaaccaac ttcttaatgg cagcatagga attacttgaa
    10081 gcacgtctta attttttaag aagctgttct ctcgtctttt caagtttttc agcttcagaa
    10141 acgcggtaaa ccaaccccca ttctagggct ttttctgccg ttaaagcctc tcctgtcata
    10201 gctaattgag cagcacgcgt cacaccgata ctgcggctca agagatgaat tccccctgca
    10261 tctggagcca aaccaacacc aacaaaggct tggataaact tagccttatc cgtcgccaaa
    10321 cagaaatctg ccgcaacagc catattcgct gcggcacctg caacagcacc gtcaacttcc
    10381 attaaaacag gtttggctat ttgcttgatt ttataagaaa tcgtattgac caactctgcg
    10441 atttttgtca atgatggaat atcatcctca tccactgctc gcttcatctc taccaaatct
    10501 cccccaactg agaagacctt tccattggca ttgattaaga taaaatgcac agctggattt
    10561 tcttctgcca aagtcagagc ttctaaaatc tcctcacaca tgggaatatg aaaaccattt
    10621 gcgacctcag gacggttcaa ggtaaggatt gccaagtcct cttcaagttg ataaataatg
    10681 tgttccatca gggctccttt ttattttata agcagtttat actcttcgaa aatctcttca
    10741 aaccgcgtca gcttccatct gcaacctcaa aacagtattt tgagctgact tcgtctagct
    10801 tcctagtttg ctctttgatt ttcattgagt gttaaattat ttcactgtca aagtatatct
    10861 tttttaggat cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaagc
    10921 tcgctttagg ctagaaagca tcttattctc ttcattgaaa aaagcctttc tttctgctat
    10981 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    11041 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    11101 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    11161 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    11221 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    11281 tagaaaaaat ttctaaaagc attcacgcct tggccactct tcctattgaa gaaaatgaat
    11341 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    11401 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    11461 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    11521 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    11581 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    11641 tcaaagctga cctctatgaa aactttacgg acgttgatgg tatctttgca gcccaccctg
    11701 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    11761 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    11821 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    11881 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagca
    11941 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    12001 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    12061 tcattctccg ttctcgccaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    12121 ttcaaaaggc taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    12181 ttggtgaaaa aatgaagagc catatcggag tgactgctac tgcgacacgc gctctatctg
    12241 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct attatgtttg
    12301 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggag
    12361 aaagtaagga agactaagca tggctcaatc acttaacaaa acagtactcc tcagtacgac
    12421 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    12481 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    12541 caatcagatt ggagccaatg tcactggacg caaaatcagc cgccacttcg aagtctttac
    12601 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    12661 cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    12721 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    12781 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    12841 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    12901 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    12961 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    13021 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    13081 taacatcttg attaaggttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    13141 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    13201 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    13261 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    13321 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    13381 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    13441 aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    13501 ctttatgttg gatgaacatg gaaaagaagg ctatactgaa gtcatcacac cttacatagt
    13561 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    13621 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    13681 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcca tgagtccgtc
    13741 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    13801 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    13861 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    13921 tgctctctct actggagata tgggcttctc agctgcgaag acttacgact tggaagtgtg
    13981 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    14041 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    14101 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    14161 ccaaaatgaa gatgcttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    14221 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    14281 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    14341 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagccatc ttgagcgagg
    14401 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    14461 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    14521 caatcaagac ccaagtcggc aggctggttc ctcctactcg aactagagaa gccaagagta
    14581 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    14641 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    14701 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    14761 tccccacata gagaaccgtc caacttggag tggcatgaac cctccgccct ggacaagcaa
    14821 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    14881 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    14941 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    15001 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    15061 gactaaaaag atgggataga accggcaacg tatctaaaat aagatttcca gctcctgcca
    15121 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    15181 atgttagttt cagtataaca taaaagcgct taaatgagga tttaaaaaaa cgaagtccgc
    15241 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    15301 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccaaaa
    15361 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    15421 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    15481 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    15541 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    15601 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    15661 atagatgaga caatttgata ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    15721 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    15781 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    15841 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    15901 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    15961 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    16021 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgactgg
    16081 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    16141 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    16201 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    16261 atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca aggaagatgg tcaagagggc
    16321 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    16381 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    16441 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    16501 ggcagctgca gctgcaatag gagcagaacc cagaccagat tcgtttgaga acacaccacg
    16561 cgccacacca ttttgaatag ccatccgaac gctagcacca gcaaatccac ctaccgcagc
    16621 aaggggacta aaagctgagg taaagactaa agcgattgtg ccagggattt ttccgatatt
    16681 aaagaaaata actgtaagag ttcctaagat ataaatgatg gccataaaag gaacaacagt
    16741 agttgaaacc ttagaaatag acttgagtcc accaaagact gcaatcgcta caaagacaga
    16801 caagacgaga gctgtgatgg ctggcgaaat cgtcgttgta ttttggatag attctgcaat
    16861 cgagttgact tgggtgaagg ttccgattcc caagagagca accaatactc ctgctactgc
    16921 aaacaaaaca gcaagtggtc gccacttttc tcccatccct agaaggatat aatgcatggg
    16981 acctcccgct actgcaccat ggtcgtcctt ggtgcggtat ttgatggcca agagtccttc
    17041 cgcatacttg gtagccattc caaagaaagc cgccatccac atccaaaata gagctcctgg
    17101 tccaccaacc ttgatagccg tcgcaactcc tatgatattt cctgttccaa cagttgatgc
    17161 caaggctgta cacagagctg caaaactgga tacatcacca tgtcccttat cctggataaa
    17221 aataagctga aaagccttgg gcagacgcaa aacctgcaag agtcctagcc gaatagttag
    17281 gtaaatccct gttccgacca ataaaatcaa gaggggcggt ccccaagcaa aagcatcgat
    17341 tgatttaagc aattctaaca tttccttctc ctatcgtttc aaccccaaaa gaaagagcac
    17401 atgcaagata catgtactct ggaatgctta gataaatgct aaaaagcggt ctatcctagc
    17461 tctgtccttt tacctgagag tttgagcagt tgcctgcctt gccccttcgg tgcctttacg
    17521 gtctctccag agttccgtcc atttacagtc atggaaaatc aaacgattcc ccacttctat
    17581 taaacttcat tcggtgttgg tatttaattg attctaattt tacaaaaaat gttggctttt
    17641 gtcaatgtgt ttattagtaa aaattagttc aacagttttt actttataaa gtccagaata
    17701 ctgctatcct ttaaaagtga caatagtcgc accactgcct ccagcatttt gtggggcata
    17761 gccgaaactc ttgacatgtt tgtttctttg caagtatttg gtaactcctt cacggatgac
    17821 tcctgttccg ataccatgga tgatatcaac ttgagccata ttgttaagca aggcttggtc
    17881 gatgaaggta tctagctcat tcatggcttc ttcatagcgc ttgcctcgaa gatccagtct
    17941 agcttgaggt cctcgcccag aagttcgttt cacaacattg acctgtttct tcttgactgg
    18001 tttttcttgc tgggcttgaa caagatcaaa ctctttctct tccaaggtca tcttaatcaa
    18061 gccaacttgg gcttcccagc ggccgtcctt gagttgactg gtcaaggtac cacgctgacc
    18121 ataactgaga accacgatat catctcccac ctttggagct cgttttttct tggccttttg
    18181 aaggaccttg tttttagaca agtccacttt ttcaggagcc aattttttca acttggcctt
    18241 ggcttcaatg atttcgtggg gcttgagttg ggatttactg tggagatttt tgagaatctg
    18301 gtcactttca cttagggcca tatccacaat ctcagcagcc tgttcacgcg ccttgttaag
    18361 ctcgatttcc ttttcacgat taagctcgtt gtagagtttt tttagcgcac ggttcatctt
    18421 gagattttct tgctccacct cacggatatt gtccaagcgt ttgcggcttt ccagcgtctg
    18481 ctcttctaat tgctcaatga tacgattgac gtcattgtcc tgatcgatct gctgactggc
    18541 atctcctacg ataacttcag ataggcctag acgtttggca atttcaaagg cattacttcg
    18601 gccaggaaca ccctgcataa agcgataggt cgggcgaaga gttgcagtat caaactccat
    18661 actggcattt tgcacaaagg ctgtctcaat accgtaggcc ttgagttctg gatagtgggt
    18721 cgtcgccatg gtcttgattt gacgcaggcg aaggtcctcc agaatagcca tggcaagggc
    18781 tgctccctct tggggatcag taccagcccc caactcatcc aaaagtaaga gtgaatgttg
    18841 gttgaccttg ccaagaatat ccacgatatt ggtcatatgg ctagagaagg tagacaagct
    18901 ctgctcaata gactgctcat ctccaatatc agcaaagatt tcttcaaaaa taccaacacg
    18961 acttcccttg tctgctaaaa tcggcaatcc tgactgggcc atgacctgtg tcaagcccag
    19021 agttttgagc atgatggtct tcccacctgt attgggacct gtaatgacaa tagctgttaa
    19081 atcttgacca aaatagacat catttgcgac ggcatttttg accaaaggat ggcagacatg
    19141 gagcagttga atctcttgat tttctgacag ctgaggcacg actgcttgtc tttcttggat
    19201 aaatcgaacc ttggcacgaa tcaagtccag atgaccgata atccaagcgt cattagcaat
    19261 ctcagccgca tgagggcgga cacgctcaga aatttcttgg agaatgcgaa gcatttcata
    19321 gcgctcatct gctcgcagac tagcaatttc ttcgctcagt ttgactacct cacgtggttc
    19381 gatatagacg gtgtttccac tagcagaaat atcatgaacg acacctgcaa tcttattgcg
    19441 gtaggtgttt ttgactggta aaacctgacg gccatttctg ctagcaacaa ttccttccgt
    19501 caacatctgc gctttttgct tgagcaagtc ttgtaaaaca tcgcgtacct gactctcgct
    19561 atcatgtatt tttcgacgga ttcgcgccaa ttcttcactg gcaaaatttt caatgaaacc
    19621 cgcatcatta aaggcctgaa gatttccttg taattgcgga aaatcatgta atttctcaaa
    19681 ccaaagggct aattcttcca agctgacatt ttccagattg gcgtaaaaac tttgaagttc
    19741 tcggctggta agaagcacgc gtttcaagag taggaactcc tcgatattga gatccgctcc
    19801 catctccaac ctcttgcaga ctcctgcaat ttccttagtt gagagaatag taaaatgcgg
    19861 ttgctcgacg aaaagagcct gcatttcttt catctcagca aaagcctgtt tgattttatc
    19921 tgctttggca gtcggagcca gctgtctcaa ttgctccaag ccctgctcgg tcaacaaatg
    19981 aggctcaaac aaggccttga ccttatcgaa ctctaatgtt tctaatattt tcttattcat
    20041 ctttatttcc ttgtctttga atgtctaggt gtggtagctt tttacattct gatagattcg
    20101 agtcaaatct gtaaacaaag ggctaggaaa actcctgccc tttttatccg attaaatttg
    20161 tcacccagat ttgtttgagc caactggttg ttatcggtat gctctggatg atgtgttttg
    20221 cgacgatact cttttcaaga ggattttgta taactgccat ggggatggtc gccaagattg
    20281 tcaaggccat ttgcaagaca aataaggtca ccaacatgga caagatacct gctgaaactt
    20341 ggaacaactt accacccagt tttttgctag gaatcaagtg taagagaaga ccaagcaaac
    20401 gaccaatgct atagacaatc ccaaatacaa gcaagtagcc gatacctgca taaaagacct
    20461 tatccagctg aaagagttga tccgatggga aaaagaaagt cccctgacct tcctgcgaat
    20521 ttgcataagg gagcaataaa tggaattgct ctccaagccc cttataaaac tggccagcca
    20581 taaaagccga tgccatggct gaaatcaggt aataaacctg taagagcagg cctctccgat
    20641 agccgatata aaatccccaa accaagacca atagaagaag gaatgaaatc ataaggaatc
    20701 ctcaatcttg ctctgttctt gcttacaagt cacaagcttg tgacggagtt cttctagctc
    20761 ttgctcctta tcgtcaaatt caatctcacg gctgagctga gttgataaac agttgactgc
    20821 caacaaaaga gcgattgttt catcatctgc gctaggcatt tgttctttaa ttgcttggta
    20881 tttttctgtc gcaaccttag cgatttcctc cataaaaagg ttgtcatgtt cgcttgtcaa
    20941 ggttaacgat tttttcccga atgtaaattt gaatcgattt agatttgcca taaaattcac
    21001 ctcacgatat tataccaaaa ttcgctaatt ttgtcagttt ttacaaattt tactgctttt
    21061 gtggtacaat agaaactatg gcaagtatca cactcacacc aagcgaaaag gatattcagg
    21121 cttttcttga acactatcaa accagtctgg ctcctagcaa gaatccctat atccgctact
    21181 ttttgaaact acctcaagca acggtttcta tctatacttc tggaaaaatc ttgcttcagg
    21241 gtgaaggggc tgaaaaatac gccagtttct ttggctatca agctgtagag caaaccagcg
    21301 gacaaaatct tcctttgatt gggacagatg aggtagggaa tggttcctac tttggtgggc
    21361 ttgcagttgt ggcttccttt gtcacacctg accagcatgc cttcttacga aaactcggtg
    21421 tgggagattc taagactctg accgaccaaa agattcgtca gattgctcct attctcaagg
    21481 aaaaaattca gcaccaggca ctccttctct cacccagcaa gtacaacgag gtcatcggag
    21541 accgctacaa tgctgtttcg gttaaggttg ccctccataa tcaggctatc tatctcctcc
    21601 ttcaaaaagg tgttcagcct gagaaaattg tgattgatgc ctttaccagt gctaaaaatt
    21661 atgacaagta cttggcacaa gagaccaatc gtttcagcaa tcctatcagc aatcctatca
    21721 gcaatcctat cagcaatcct atcagcaatc ctatcagctt agaagaaaag gctgagggca
    21781 aatacttggc tgtcgcagtt tcttctgtca ttgcgcgtga tctctttctg gaaaatcttg
    21841 aaaatttggg acgagaactg ggttatcagc ttccaagtgg agctggaacg gcttctgaca
    21901 aggtggctag ccagattttg caagcctatg gtatgcaggg actcaacttc tgcgccaaat
    21961 tgcactttaa aaatactgaa aaagcgaaaa acgcttagaa aggtaagtta tgaatttatt
    22021 taaaaatttc ttaaaagagt ggggattatt cctcctgatt ctgtcattac tagctttgag
    22081 ccgtatcttt ttttggagca atgttcgcgt agaaggacat tccatggatc cgaccctagc
    22141 ggatggtgaa atcctctttg ttgttaagca cctccctatt gaccgttttg atatcgtggt
    22201 ggcccatgag gaagatggca ataaggacat tgtcaaacgt gtcatcggaa tgcctggcga
    22261 taccatccgt tacgaaaatg ataaacttta catcaatgac aaagaaacgg acgagcctta
    22321 tctagcagac tatatcaaac gcttcaagga tgacaaactc caaagcactt actcaggcaa
    22381 gggctttgaa ggaaataaag gaactttctt tagaagtatc gctcaaaaag cccaagcctt
    22441 cacagttgat gtcaactaca acaccaactt tagctttact gttccagaag gagaatacct
    22501 tctcctcgga gatgaccgct tggtttcgag cgacagccgc cacgtaggta ccttcaaagc
    22561 aaaagatatc acaggggaag ctaaattccg cttctggcca atcacccgta tcggaacatt
    22621 ttaagagacc taagaggccg agaatcacca atctcagcct cttcttctat cgtgagaaaa
    22681 tgattggtaa ctatctaaac ttaccagaac agaaacacct caactctcac ctattcatgc
    22741 aaaggaattt tatggaagtt tatttttcag gaaccattga acggattatt tttgaaaatc
    22801 ccagcaattt ttatcgcatc ctcctcctag aaatcgacga tacggacgca gaggattttg
    22861 atgattttga aatcattgtc acaggaacca tggctgatgt aattgagggc gaagactata
    22921 ctttttgggg gcaaattgtc cagcactcca agtatggaga acaactgcaa atcagtcgtt
    22981 atgatcgcgc aaaaccaact agtaagggct tggtcaagta cttttcaagt agccatttca
    23041 aggggattgg tctcaagaca gctcagaaaa tcgtggatac ctatggcgaa aataccattg
    23101 acgaaatttt gcaacaccca gaaaagttag aaggcatcgc aggactctct gccaaaaatc
    23161 gcgaggcttt cgtctccact ctccgtctca actacggaac ggagatggtt ttggccaaac
    23221 tagccaacta cggcattccc aacaaactag cctttcagat tcaagacttt tacaaggaag
    23281 aaacccttga tgtggttgaa aattatccct accagctggt tgaggatatc aagggtttgg
    23341 gctttaccat tgctgaccaa ctagctgagg aactaggcat cgaaagtcag gctcctgaac
    23401 gcttccgcgc cggtctagtt cacagtcttt ttcaggcctg tatggaaaca ggggacacct
    23461 atgttgaagc acgggatttg ctggaacaaa cccttactct ccttgagtct tcccgtcccg
    23521 tggaactaga ccccagccaa gttgcccaag agctctccta cctgatcgaa gaagacaagg
    23581 ttcagcagat tgataccaag atctttgaca acagcctctt tttcgctgag gaaggcatcc
    23641 gcagtcactt ggttcgtatc cttgaaaaag gaaaacagaa gagtcatgat ttaaaaacca
    23701 ttcaaaaaca tatcactact atcgagaaag agcttggaat tgagtatgat aacattcaaa
    23761 aacaggctat ctgtgatgct atccagaata aggtctttat cctgacaggt ggacctggta
    23821 ctggtaagac aactgttatc aatgggatca tcgctgttta cgccctgtta gagggacttg
    23881 acctcaggaa gaaaagcaac ctgcccattc ttcttgctgc tccaactggt cgagctgctc
    23941 gtcgcatgaa tgaattgaca ggtttgccta gcgcgaccat acaccgccac ttgggaatga
    24001 caggtgacga tgataccagt catctggaag attacctaga tgctgacttt atcatcgtgg
    24061 atgaattctc tatggtggat acttggctgg ccaatcaact cttctccagt atctcttcta
    24121 acagtaagat cctcatcgta ggtgacagtg atcagctacc ttctgtcagt cctggacagg
    24181 ttctagcgga tctgcttcat attcctttga ttcctcagac tcgcttggaa aaaatttatc
    24241 gacaaagcga agaatcaacc atcgtcaccc tagctagtca gattcgacag ggcatcttgc
    24301 cagctgattt cacccaaaaa aaagctgacc gttcctactt tgaaattgct agtggccata
    24361 ttcctgccac cattgaaaaa atcttaggtg ccgccctcag aaatggtatt cctgcccgtg
    24421 atatccaagt tctggctcct atgtaccgag ggacggcagg gattgatgct atcaatcagc
    24481 tcatgcaaga cctgctcaat cccccacaaa aagatcaact cagttttgaa gctcctcagt
    24541 gccactatcg taagggagac aaggtcattc atttggttaa cgatgctgaa atcaatgtct
    24601 ttaatgggga tttaggagct atcacagacc tgattcctgg taaatacacc gaatcgaaac
    24661 aagacgagat tgtcattgat tttgatggta atgaagtctc ttacccccgt aacgaatggt
    24721 acaagattcg cttggcctat gccatgagta ttcataagtc tcagggaagt gagttcccag
    24781 ttgtcatcct accgattact agtgctagcc gacgtatgct ggagcgaaat ctcatctaca
    24841 cagccattac acgtgccaaa agcaaactca tcttactagg cgaattacaa gccttcgact
    24901 atgctaccca acacatcgga actgcccgaa aaacctatct gattgaacgc ttcagtgatc
    24961 tactggagaa tgttgaagaa aagcaacaag ctgtctctga aacagtcaca tcaagtgcct
    25021 ctgaacaatc ctacatccta accgaagaaa actgggacag cattccagcc atgattggga
    25081 ttacagacac agacctcaaa gagatttttg gaaaatagta catcagaaaa cccaccaatt
    25141 caggtgggtt ttctgtttat taagattatt ttactgttgc tgtgcttgtg atcaattcaa
    25201 cagctttttt gatagtgata tcatgtttca acatgtcagc tgaaagcaag ttttgaactt
    25261 gtgcaacttc catgttgtag tctgctgcca attgctcaac ttctttttgg atttcttctt
    25321 ctgaagcatc aaatccttca gctttggcaa ctgcttcgat aacaaggtta gtcttagtac
    25381 gtgactcagc ttctgcttgg tattggttgt gaaggtcttc ttgagtagtt ccagtgattt
    25441 ggaagtacat gtcagggttg atcccttgac gttgcaagtt cccaaggaat tcatttactg
    25501 aacggtgaac ttcttcatgg atcatttctt ctggaagttc tacgatttca gcgttttcta
    25561 cagctgtatc aattgctgca ccttcaactg catctttgta agcttcttct ttagcagcag
    25621 ccaattcttt gcggtatttt tctttcaagt cagcaagtgt ttcaacttct tcatcaatgt
    25681 cttttgcaag ttcatcgtca agagctggaa cttctttagc ttttacttcg tggatagttg
    25741 tcacgaattt agcttcttta cctgcaaggt cttctgcttg gtagtcttct gggaatgtta
    25801 cgataacatc aacggtttcg ccagctgagt gacctaccaa ttggtcttcg aaaccaggga
    25861 tgaattgacc tgaaccaagt ccaagtgaga agttttcacc ttttccaccg tcaaattcaa
    25921 caccgtcgat agaaccaacg aagtcgatca caacagtgtc gccgttttca gcagcagctt
    25981 ccttgataac caattcagcc aggttgttgc gttcgcgttc gatacgctct tcaacatcag
    26041 cgtcagttac ttctttttct acatcaactg atacttcaag gtttttgtag tcacccaatt
    26101 ttacttcagg ttttgtaacg acttcagcag cgataaccca gtcttgacct ttttccattg
    26161 aagttacgtc aatttttggt tgggcaacca cttcaagacc agcttctttt acagctgctt
    26221 cataagcgtt tggcaaaaga gcgttcataa cgtcttggta aagtgattct tcaccaaatt
    26281 ttttatcgaa gataggacgt ggaaggtgac ctttacggaa acctggaaca ttaagagatt
    26341 tcttcactga cttgaagaca cggtccaatt ctggtttgat ttggtcttga gagatagtga
    26401 aagtcaagac accacggttt gtttctttgt tttcaaatga tacagacatt ctgtcatttc
    26461 tccttaaaat tttt
//
LOCUS       DAVMAF010000021        26394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMAF010000021 DAVMAF010000000
VERSION     DAVMAF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.021"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            48..443
                     /locus_tag="VYO97_000895"
     CDS             48..443
                     /locus_tag="VYO97_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1753716.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            454..825
                     /locus_tag="VYO97_000896"
     CDS             454..825
                     /locus_tag="VYO97_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1753717.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            835..3078
                     /locus_tag="VYO97_000897"
     CDS             835..3078
                     /locus_tag="VYO97_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1753718.1"
                     /translation="MTEIVKANLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEAIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3285..5060
                     /gene="spxB"
                     /locus_tag="VYO97_000898"
     CDS             3285..5060
                     /gene="spxB"
                     /locus_tag="VYO97_000898"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1753719.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5171..5518
                     /locus_tag="VYO97_000899"
     CDS             5171..5518
                     /locus_tag="VYO97_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1753720.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5635..5876)
                     /locus_tag="VYO97_000900"
                     /pseudo
     CDS             complement(5635..5876)
                     /locus_tag="VYO97_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6047..6328
                     /locus_tag="VYO97_000901"
     CDS             6047..6328
                     /locus_tag="VYO97_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1753721.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            6460..7404
                     /gene="manA"
                     /locus_tag="VYO97_000902"
     CDS             6460..7404
                     /gene="manA"
                     /locus_tag="VYO97_000902"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1753722.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDTLLTKVPVKAG
                     DFFYVPSGTMHAIGTGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADNLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLFSILAGQGQL
                     TVDGKNYPIQKGSHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(7444..8361)
                     /locus_tag="VYO97_000903"
                     /pseudo
     CDS             complement(7444..8361)
                     /locus_tag="VYO97_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            8400..9204
                     /locus_tag="VYO97_000904"
     CDS             join(8400..8768,8770..9204)
                     /locus_tag="VYO97_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1753723.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFKEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGFTVSR
                     TPLSSEDTVMIDATEVKINRPKKQLANDSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     SHDMKLFKMSRRNIEQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKAYNHA
                     LSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(9201..9446)
                     /locus_tag="VYO97_000905"
     CDS             complement(9201..9446)
                     /locus_tag="VYO97_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1753724.1"
                     /translation="MEQSPQKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(9461..10633)
                     /locus_tag="VYO97_000906"
     CDS             complement(9461..10633)
                     /locus_tag="VYO97_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1753725.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGANPI"
     gene            complement(10692..11537)
                     /locus_tag="VYO97_000907"
     CDS             complement(10692..11537)
                     /locus_tag="VYO97_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1753726.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            11662..12219
                     /locus_tag="VYO97_000908"
     CDS             11662..12219
                     /locus_tag="VYO97_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1753727.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            12238..12705
                     /locus_tag="VYO97_000909"
     CDS             12238..12705
                     /locus_tag="VYO97_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1753728.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            12794..13423
                     /gene="upp"
                     /locus_tag="VYO97_000910"
     CDS             12794..13423
                     /gene="upp"
                     /locus_tag="VYO97_000910"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1753729.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            13597..14187
                     /gene="clpP"
                     /locus_tag="VYO97_000911"
     CDS             13597..14187
                     /gene="clpP"
                     /locus_tag="VYO97_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1753730.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            14266..14514
                     /locus_tag="VYO97_000912"
     CDS             14266..14514
                     /locus_tag="VYO97_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1753731.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            14615..15775
                     /locus_tag="VYO97_000913"
     CDS             14615..15775
                     /locus_tag="VYO97_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1753732.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            16043..16912
                     /locus_tag="VYO97_000914"
     CDS             16043..16912
                     /locus_tag="VYO97_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1753733.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            16916..17872
                     /locus_tag="VYO97_000915"
     CDS             16916..17872
                     /locus_tag="VYO97_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1753734.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            17872..18636
                     /locus_tag="VYO97_000916"
     CDS             17872..18636
                     /locus_tag="VYO97_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753735.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            18636..19346
                     /locus_tag="VYO97_000917"
     CDS             18636..19346
                     /locus_tag="VYO97_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753736.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            19654..20310
                     /locus_tag="VYO97_000918"
     CDS             19654..20310
                     /locus_tag="VYO97_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1753737.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            20418..21513
                     /gene="prfB"
                     /locus_tag="VYO97_000919"
     CDS             join(20418..20489,20491..21513)
                     /gene="prfB"
                     /locus_tag="VYO97_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1753738.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            21531..22223
                     /gene="ftsE"
                     /locus_tag="VYO97_000920"
     CDS             21531..22223
                     /gene="ftsE"
                     /locus_tag="VYO97_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1753739.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            22216..23142
                     /locus_tag="VYO97_000921"
     CDS             22216..23142
                     /locus_tag="VYO97_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753740.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(23323..24276)
                     /locus_tag="VYO97_000922"
     CDS             complement(23323..24276)
                     /locus_tag="VYO97_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1753741.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            complement(24499..25254)
                     /locus_tag="VYO97_000923"
     CDS             complement(24499..25254)
                     /locus_tag="VYO97_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753742.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKFESSIMKLATLP"
     gene            complement(25251..26192)
                     /locus_tag="VYO97_000924"
     CDS             complement(25251..26192)
                     /locus_tag="VYO97_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1753743.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
BASE COUNT         8026 a         4892 c         5628 g         7848 t
ORIGIN      
        1 tatactgatg agtgtaattg acaaatgtag attttggagg tatggtaatg cagatttcag
       61 atgcagaatg gcaggtcatg aagattattt ggatgcaggg ggagcagact agtacagatt
      121 tgattagggt tttggcagag cggtttgact ggtccaagtc cacggttcag acacttttgg
      181 ctcgtctggt tgagaaagag tgtttgactc ggaaaaaaga aggcaagtcc tttgtttatt
      241 cagccctttt aactctagac caaagtcggg atttacttgt ccaagatatc aaagacaagg
      301 tttgttcccg taggattagg aatttgttgg ctgacttgat tgtagaatgt gaatttactc
      361 agactgactt ggaagacttg gaagctgtga tttcagagaa gaaatcaagt gctgtaacag
      421 aagtaagatg taattgtatg taaaggagac gtcatgttaa atagtattgt aaccattatt
      481 tgtattgccc ttatcgcatt tatcttgttt tggtttttca aaaagcctga aaaatctgga
      541 caaaaagccc agcaaaaaaa cggataccaa gagattcgag tggaagtcat gggaggctat
      601 actcctgagt tgattgtcct aaagaaatca gtgccagcac gcattgtctt tgaccgcaag
      661 gatccttcac catgtctgga tcaaattgtt tttccagatt ttggtgtaca tgcgaacctg
      721 ccaatggggg aagagtatgt agtggaaatc acgcctgaac aggctggaga gtttggcttt
      781 gcttgtggta tgaacatgat gcacggcaag atgattgtag agtaggtgga gactatgaca
      841 gaaattgtga aagcaaactt agaaaatggc attcaaaaaa tccgtatccg agctgaaaaa
      901 ggctatcatc cagcccatat ccagcttcaa aagggaattc cagctgagat tacctttcat
      961 cgtgctactc cttcaaactg ttataaggaa attctgtttg aagaagaagg tatcttggaa
     1021 gcaatcggcg tagatgagga gaaagtcatt cgttttacgc ctcaagaatt agggcgacat
     1081 gaattttctt gtggcatgaa gatgcaaaag ggaagctata cagtcgttga gaagactcga
     1141 aaatctctat ctctcctgca acgtttttgg attactagta tctttactgt gcctcttgtg
     1201 attctcatga ttgggatgtt gacaggtagc attagtcatc aagtcatgca ttggggaacc
     1261 tttctagcaa caacgcctat tatgttagtt gcaggtaagc catatatcca gagtgcttgg
     1321 gccagtttta aaaagcacaa tgccaacatg gataccttgg ttgcgctggg aactctagtg
     1381 gcttatttct atagcctagt tgctctcttt gctggtctcc ctgtttactt cgaaagtgct
     1441 ggatttatcc tctttttcgt tcttttggga gcagtttttg aggaaaaaat gagaaaaaat
     1501 acgtcccaag ctgtggagaa attactggac ttgcaagcta aaaccgcaga agtcttgagt
     1561 gatgatagtt atgtccaagt tcctttggaa caagtcaagg taggcgacct gattcgagtg
     1621 cgtcccggtg aaaagattgc tgttgatggt gtcgtagtag aaggtgtctc tagtattgac
     1681 gaatccatgg tgacaggtga gagtctgcct gtggacaaga cagttggaga tactgtcatt
     1741 ggctcaacca tcaatcatag tggaacgctt gtctttagag cagaaaaagt tggctcagag
     1801 actgttttgg ctcagattgt ggattttgtg aagaaagctc agacaagtcg tgcgccgatt
     1861 caggacttga cggataagat ttcagggatt tttgtcccag tagttgtcat tttaggaatc
     1921 atgacctttt gggtttggtt cgtcttgctc agggatagtg tggtcgtgct tggagctagc
     1981 tttgtgtcct ctcttctcta cggagtggcg gttttgatta tcgcctgtcc ttgtgccttg
     2041 ggacttgcaa caccgacagc ccttatggtg gggacaggac gtagtgccaa gatgggggtt
     2101 ctcctcaaaa atggaactgt cttacaggaa atccagaaag ttcaaactct tgtctttgat
     2161 aagaccggga ctttgacgga agggaaacct gtggtaacag atatcatcgg cgacgaagta
     2221 gaagtgtttg gattggcagc ctccttggaa gatgcttctc aacacccact ggctgaggct
     2281 atcgttaagc gagcgagtga agctggactt gagtttcaaa ctgttgaaaa ttttcaggcc
     2341 ttgcacggga aaggtgtttc agggcgaatc aatggaaaac aagttttact tggaaatgct
     2401 aaaatgctgg atggcatgga tatttctaat acttatcaag ataaactaga agaactagaa
     2461 aaagaagcta agacagttgt gtttttagct gttgacaatg aaatcaaagg cttgcttgct
     2521 ttgcaagata ttcctaagga aaatgctaag ctagccatca gtcagctaaa aaaacgtggt
     2581 ctccgaacag tcatgctgac aggagacaat gctggtgtgg cgcgtgctat tgcagatcaa
     2641 atcggaattg aagaggtcat tgcaggcgtc ttgccagaag aaaaagccca tgaaatccat
     2701 aaactgcaac agtcagggaa agtagccttt gttggggacg gtatcaatga cgctcctgcc
     2761 cttagtgtag cagatgtggg gattgctatg ggagctggaa cagatatcgc catcgagtca
     2821 gcagatttgg tgttgacaac caataatctt ttaggagtgg ttcgtgcctt tgatatgagt
     2881 aagaaaacct ttcatcgaat tctactcaat cttttctggg cttttatcta caatgttgtc
     2941 ggaattccga ttgcagcagg agtcttttca ggtgttggac tggctctcaa cccagaattg
     3001 gcaggtctag ccatggcctt tagttctgta tctgttctga ccagttccct tctgttaaat
     3061 tttagtaaaa tagactaaaa gtaatgacta gatttctttg ttataaaaca gaaatgacag
     3121 aactttaaaa actgtattag aatagagaat agagagtttt gagcagattt ttagaaaagt
     3181 cagcataaat atgatacagt ggaatagtaa aaatttggag aacgtttcca attctatgta
     3241 atcgtattct ccaaatctaa aaaaattgaa ggagagttat cattatgact caagggaaaa
     3301 ttactgcatc tgcagcaatg cttaacgtat tgaaaacatg gggcgtagat acaatctacg
     3361 gtatcccatc aggaacactc agctcattga tggacgcttt ggctgaagac aaagatatcc
     3421 gcttcttaca agttcgccac gaagagacag gtgctcttgc agcggttatg caagctaaat
     3481 tcggcggctc aatcggggtt gcagttggtt caggtggtcc aggtgcgact cacttgatta
     3541 acggtgttta cgatgcagct atggataaca ctccattcct agcgatcctt ggatcacgtc
     3601 cagttaacga attgaacatg gatgctttcc aagagcttaa ccaaaaccca atgtacaacg
     3661 gtatcgctgt ttacaacaaa cgtgtagctt acgctgagca attgccaaaa gtaattgacg
     3721 aagcctgccg tgctgcaatt tctaaaaaag gtccagctgt tgttgaaatt ccagtaaact
     3781 tcggtttcca agaaatcgac gaaaactcat actacggttc aggttcatac gaacgctcat
     3841 tcatcgctcc tgctttgaac gaagttgaaa tcgacaaagc tgttgaaatc ttgaacaatg
     3901 ctgaacgccc agttatctat gctggatttg gtggtgttaa agctggtgaa gtgattactg
     3961 aattgtcacg taaaatcaaa gcaccaatca tcacaactgg taaaaacttt gaagctttcg
     4021 aatggaacta tgaaggtttg acaggttctg cttatcgtgt tggttggaaa ccagccaacg
     4081 aagtggtctt tgaagcagac acagttcttt tccttggttc aaacttccca tttgctgaag
     4141 tttacgaagc attcaagaac actgaaaaat tcatccaagt tgatatcgac ccttacaaac
     4201 ttggtaaacg tcatgccctt gacgcttcaa tccttggtga tgctggtcaa gcagctaaag
     4261 ctatccttga caaagtaaac ccagttgaat caactccatg gtggcgtgca aacgttaaga
     4321 acaaccaaaa ctggcgtgat tacatgaaca aactcgaagg taaaactgag ggtgaattgc
     4381 aattgtatca agtttacaat gcaatcaaca aacatgctga tcaagacgct atctactcaa
     4441 tcgacgtagg taacactact caaacatcta ctcgtcacct tcacatgaca cctaagaaca
     4501 tgtggcgtac atctccactc tttgcgacaa tgggtattgc ccttcctggt ggtatcgctg
     4561 ctaagaaaga caatccagat cgccaagtat ggaacatcat gggtgacgga gcattcaaca
     4621 tgtgctaccc agacgttatc acaaacgttc aatacgacct tccagttatc aaccttgtct
     4681 tctcaaatgc tgagtacggc ttcatcaaga acaaatacga agatacaaac aaacacttgt
     4741 ttggtgtaga cttcacaaac gctgactacg ctaaaattgc ggaagctcaa ggagctgttg
     4801 gattcacagt tgaccgtatc gaagacatcg atgcagttgt tgcagaagct gttaaattga
     4861 acaaagaagg taaaactgtt gtcatcgatg ctcgcatcac tcaacaccgt ccacttccag
     4921 tagaagtact tgaattggat ccaaaacttc actcagaaga agctatcaaa gccttcaagg
     4981 aaaaatacga agcagaagaa ctcgtaccat tccgtctctt cttggaagaa gaaggattgc
     5041 aatcacgcgc aattaaataa ttcctctcgc cgaaaatcaa atatgaaact tgtagacttt
     5101 attctttgat tttcatagag cagaacttta aaagatcgga gcaatccggt ctttttatgg
     5161 aggagagtaa atgaatttaa atcaattaga tattatcgtt tcaaatgttc cccaagtctg
     5221 tgctgacttg gagcatatct tggataaaaa ggcagattat gctgatgatg gttttgctca
     5281 gtttacgatt ggtagtcact gccttatgtt gtcacaaaat catttggttc ctttggaaaa
     5341 ctttcagtca ggaatcatta ttcatatcga ggttgaggat gtagaccaga actacaaacg
     5401 gttgaacgag cttggtataa aggttttaca cggaccaact gtaaccgatt ggggaacaga
     5461 gtccttatta gttcaaggac ctgctggtct agtgcttgat ttttatcgta tgaaataggt
     5521 cgcgtagtca tttgacgtta accttagcta tttttaaaat agaaattgag aatatgaact
     5581 attgaaagat cggagcaatc cggtcttttt atataaagaa attgcatata atactcatct
     5641 gctttcaaaa agtattctag tccatctctg attaacgatg gactttatca cctccttctc
     5701 cagtccttgt atgacatctt ggagttgatt catgacatct tccaaagttc gaaaggcttt
     5761 attcttaaat ccacgtttat gaatctcttt ccacacttgt tcgatgggtt catctctggt
     5821 gtgtatggag gaataaatgc aaaaccaata ttagtcggag tctttataag atttgcccat
     5881 cagacgaaat agaatgattt gaaggcattt atgatattta gctgtacgag agtctttaaa
     5941 aagtgttttg atggtttgga tttcttcttt agttgatttc atatcactat tatataatgc
     6001 tttttgattt tagtatagta ttatcgtaga aaatggactc ggtgctatgg atacaccgga
     6061 tgagaatggt tatgtagcag atgactaccg gattacttac ttagaggccc acatcaaggc
     6121 catgcgagat gccatttaca aagacggggt tgacttgctt ggttatacga catggggctg
     6181 tatcgattcg gtttcagctg gcacagggga aatgaataag cgctatggct ttatctatgt
     6241 agatcgagat aatgtaggaa atgggactct caaacgtagc aagaagaagt ccttctattg
     6301 gtacatgtca tttatagcaa tggtgtaagc atcgagtaga gcacatttgt ttactatttt
     6361 tataaaatct tctccctgct ttataagatg taaaaaagag ttcaaatgga actctttttt
     6421 tgctataatg aggggagaaa aatcagacag gagattaaga tgtcagaacc attattttta
     6481 caatcagtta tgcaagaaaa aatctggggt ggtaccaagc tacgtgatga gtttggctac
     6541 gacatcccaa gtgaaaaagt cggagagtac tgggctatct cagctcatct aaatggtgtt
     6601 tctaaagttg ccaatggtcg ttacgaggga acagatcttg ctactttgta tgcggaacac
     6661 cgtgaattat ttggcaatcg tcctgagcct gtatttccgc ttttgactaa gattttggat
     6721 gctaacgact ggctcagcgt tcaagttcac ccagacgatg cttatggact agagcatgaa
     6781 ggcgaacttg gaaaaacaga atgctggtac atcatcgctg cggatgaagg ttcagagatt
     6841 atctacggcc acaatgccaa gtcaaaagaa gaactccgcc agcaaatcga ggacaagaac
     6901 tgggatacct tgcttaccaa agtccctgtc aaggctggag atttcttcta tgtaccaagt
     6961 ggcaccatgc atgctatcgg tactggcatc ttgattcttg aaacccaaca gtctagtgat
     7021 acaacttacc gcgtctatga ctttgaccga aaggacgata agggcaactt gcgtgaactt
     7081 caccttgaaa aatccatcga tgttttgaac attggcgagc cagcaaatag ccgtcctgta
     7141 actgttaaag cagataattt gcgttccact ttacttgttt ctaatgattt cttcgcagtt
     7201 tacaagtggg aaatcactgg aaaagttgat tttgagaaga cagctgatta cagtttgttt
     7261 agtattttag ctggccaagg tcaactgact gttgatggaa aaaactatcc aatccaaaaa
     7321 gggagccact ttatcctacc aagtgatgtt gaatcttgga ctttagaagg gcaagattta
     7381 gaattaattg ttagtcatcc ataaaaaaga aagggcctga gttcattact caagtccttt
     7441 tgattacata aattgggcga tgaagaccac gatgatgatt ggaatgacga aacgaagaag
     7501 gaagagccag acttggaaca gtccttgttt ccatgctctt tcatcgagat ggagttcctc
     7561 cattgcaaga gcctttttaa agatatagcc tgtaaaaagt gaaaggcaga gagctccaaa
     7621 tggcatgaga agattggaaa ccaagaaatc catggcatca aagaaagttt taccaaagat
     7681 gtgaacatcc gccataactc cataggatag ggctgaagga atgccaaaga caaaggtcaa
     7741 aattcctaaa ataacactcc atttggcacg tttgctgttg tcctgattgg tgatattgtc
     7801 tacattgatt tccagcataa cgacagaaga agtgacagtc gcaaagagga agaggacata
     7861 gaaaatggtt ccaaaaggca tcttgtcaaa gagttgaggc aagacgataa agaccaagct
     7921 cggtccccct tcagactgga tattgaaggg ggaccgagct tggaaaatgg ctagacctgc
     7981 catgatggat accgagatat tcatggctac gatggagatt cttgactgga ccagattggt
     8041 tttcttgtcc aagtaagaag cataggtcaa catggctgta acccctagtg agagggcaaa
     8101 gaaagattgt ctcagagcat agaggagacc agcgctagtc agttttgaaa agtctggttt
     8161 gaggaagtaa agaacccctt ccatggcatt tgacaagctg agagagcgac cgatgataac
     8221 aacaaagatg ataaagagca ggggcatcat gactttcgaa gctctttcaa tccctttttg
     8281 aaccccacgt gatacaatga agatattcaa taggataaag gccgcttgag ctcctagggc
     8341 aatggctgga tttgaaatga tcagacttcc tgcgaaacaa aatatggcat agtagttcta
     8401 tgaatgatga agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc
     8461 agcgcacgac ttttaaagag atgttagctg tattaaaaac agcttatcaa cttaaacacg
     8521 caaaaggtgg acgaaaacct aaattaagcc tagaagacct tcttatggcc actcttcaat
     8581 atgtgcgaga atatcgaact tatgaagaaa ttgcggctga ttttggtatc catgaaagca
     8641 acttactccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt tttacggttt
     8701 caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa gtaaaaatca
     8761 atcgccctaa aaaaacaatt agcgaatgat tctggtaaaa agaaatttca cgctatgaag
     8821 gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc tgtgaactat
     8881 agtcatgata tgaagttgtt caaaatgagt cgtagaaata tcgaacaagc tggtaaaatc
     8941 ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
     9001 aaatccagca aactcaagcc gctaacagct gaagataaag cctataacca tgcgctatct
     9061 aaggaaagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatattttca
     9121 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgag tgctggtatt
     9181 atcaatcatg aactaggatt ctagttttgc aggaagtcta atagagtatg aacctgttct
     9241 cgtatttctt cgagagctaa gttactttca agaataatat ttccactttg aatataagtt
     9301 cgaacttgtt gaaaatcagc ttctgtcaag atatctacta gataagacat tttagggata
     9361 gcattttttc cattaggagt gactcccctt agcaagataa tatgttccaa agtagttcct
     9421 ttcttttttt ggggggattg ttccatttag taatgtatat ttagattgga tttgctcccg
     9481 ccacccagcc ggtacagagg gcagaagtga tgttaaagcc acccgtgtgg gcattgatat
     9541 ccataacttc gcctgcaaag tggaggccag gtaccagctt actttcaagg gttttaggat
     9601 tgatttcctt gagactgact ccacccttgg taacaaagga ctttgcaagg gacatttttc
     9661 cagttacagg aattttaagt tctttaatgg actgaacaag ttgttctcgt tccttttcag
     9721 tcagctgttt gactttttca ggatatcctt gtacaaaaaa ttcggccaag cgttctggta
     9781 acaaggtttt taaagcgttt ttcaaggatt tttcccgatt ttcttctaga aatgtaacca
     9841 agtccttctc agaaagttga ggcaaaacat cgagtgagag aacctcccca cctttgacaa
     9901 agctagacat gcgtagggca gcaggacctg acaaaccaaa gtgggtaaag agtaaatcat
     9961 gagtgatgac atgcttacca taacttaggg tcacatcgtc cagagaaatc ccttgtaagg
    10021 ctttatgtgg aaaatctgtt aataaaggac tttcagcagc ctcaagctcg gtgatggtat
    10081 gcttaaaatg gcgagcaatc tcgtgaccaa aaccagtcga accagtcgaa ggataagact
    10141 taccacctgt tgtgacaatg agtttctcac aagtgaaggt ttgatccgct gacttaagga
    10201 caaactggtc atctactttt ttaacagaaa cgatttctat ttgagtagca acttgaccac
    10261 ctagttcggt gattttcttt tccaaagctt cgataatagt ccgagacttg tcactggctg
    10321 gaaagacgcg tccgtggtct tcgaccttaa gtttaacacc attttctgta aaaaagttga
    10381 tgatgtcatg attatcgaac tgggagaaaa cactgtaaag aaagcgtccg tttccaggaa
    10441 ttccagctag caggttgtct aagctaccat tgttggtcac attgcaacgt cccccaccag
    10501 tcccagctaa tttttttcca agtttccgat ttttttcgat gaggagggtt ttctgtccat
    10561 aaaagctact ggaaatcgta gccatcatac cagcaggtcc cccaccgatg acaatagtat
    10621 caaaatgttt catagctcta ttgtaccaca aaaaaacaag agatgatggt cacctcttgt
    10681 caagaatgca attaatcaat ttcatagccc atcagcaaac cgccctcttc tgcatagaaa
    10741 ctgcagagac cagaggttgg tagaatttta atatccgctt gtgggaaggt ttcacggatt
    10801 cgctctgaga gctgttgaca acatttttcg ttattgcgtt gggccatgac aatacggcca
    10861 ccagcatatc cagcttttac taactcatca taggcagctt gaactgattt ctttgatccc
    10921 cttgcttttt gtagcaattc gagagttcca gtttcactag cttttccgac catacgaatg
    10981 ttgagaaggc caactaccgt accgataagc ttactcaaac ggccgttctt caccaagtta
    11041 tcgactttgg ctaggacaaa gagcaactta gttttttctt gataggcggt gatagcttca
    11101 accacttctt caaaagacaa gccttggtca atcaagtcat tcaatttttc tacgagtagg
    11161 tcaacttcac caccagcaga taaactatca atcacatgaa tcttagtgtc aggatggtct
    11221 tccagataaa tattctttgc tagttgagcg ctattgtgac tgccagaaag ggtacctgtg
    11281 atggttacta ggaaaatgtt tttggcacct tcaaatgctc gcaaatagtc atctgggctt
    11341 ggacaagctg attttgaagc ttctgcagtt gcatacatgg tttccatcat ttggtcaata
    11401 tcgagactgg cgtcatcaac aaagacctga tcagctactt gaatggttaa ggggacactt
    11461 acaaaggttg tgttaatagc tggtgttggc agttgacgat aatcacaacc agagtcagca
    11521 ataatcttcc aagtcataga aattctccat ctttgtcagt tatacattga caaaggttct
    11581 gtcttttttt acaattatat catgaaaact cttgaaacaa aagcatcatc cgtctgttgt
    11641 gacaagtaga aagaaaatgt tatgtctgaa cgtagaatct ctgaaaagtc tcttgaaaat
    11701 ctcagaaaat caaaccaaga atccaattta ttaaccagag aagccattga aacagccctc
    11761 ttgcaactct tggaaaaaaa ggagctgacc aagattagta tttctgaatt ggtcaaacgt
    11821 gcaggtgttt cgcgtgcggc cttttatcgc aattatgact ccaaagagga aattttagag
    11881 agcgtcttta aacgaactgt ccacaatatt atggaacagc tgcatcatta cgatttaaag
    11941 acagaccttt atttggtttg ggttcacctt ttccgggagg ccagaaagga agctagagta
    12001 attcaattgg ccttggatta ccatctggaa aaaatctttg tccaagccat gcaggaattt
    12061 ctagaaaaat accatgggaa atcaaaaggt gtcagctctt atcttcattc cttctggagc
    12121 tcagccatcg tctctgtcct tctaaaatgg atcaaggatg gcatgaaggt accagctgaa
    12181 aagattgcag atttaggttt accatttttt aaaaaataga gaaaaaggag aaaagagatg
    12241 actgaaaaaa gactggcctg ggatgagtat tttgcagccc aagccctact aattgcgaat
    12301 cgttccactt gtaaacgtgc taaagtgggc gcgattctgg taaaagataa taaggttatt
    12361 tccactggtt acaatggttc ggtgtcaggg actgagcatt gtattgatca cgaatgtttg
    12421 gtcattgaag gccactgtgt tcgcaccctt cacgctgagg tcaatgctat ccttcaaggt
    12481 gctgagcgtg gtgttcccaa aggctttaca gcctatgtaa cccattttcc ttgtctcaac
    12541 tgtacaaaac aattgcttca ggtcggctgc aagcgcgtgg tttatatcaa ccagtaccga
    12601 atggacgact acgcccaata cctttatcaa gaaaaaggga cagaattgac ccatttacca
    12661 cttgagacag tacagacagc tcttaaagag gctgatctaa tgtaaaaatt atcaaaaata
    12721 aatggcttag aaagattttt aaaccgtttt ttggtataat aagaagaata aattgaaaga
    12781 aggaattcca aaaatgggaa aaattgaagt tattaatcac ccactgattc aacacaaatt
    12841 gtcaatcttg cgtcgtacag atacttctac aaaagctttt cgtgagctag tagatgagat
    12901 tgcaatgttg atggggtatg aagtacttcg tgatcttcca ctagaagatg tggaaatcga
    12961 aacaccaatt acaaaaacag ttcaaaaaca attggcaggt aagaaattgg ccatcgtccc
    13021 aatcttgcgt gcaggtatcg ggatggttga tggtctcttg agcttggttc cagctgctaa
    13081 agttggccac atcggtatgt accgtgatga agaaacactt caaccagttg aatacttggt
    13141 gaaattgcct gaggacattg accaacgtca aatttttgta gtagacccaa tgttggcaac
    13201 aggtggctca gcaatcttgg ctgttgattc tcttaaaaaa cgtggcgcat caaatatcaa
    13261 atttgtctgc cttgtatctg ctccagaggg tgtaaaagcc cttcaagaag ctcatccaga
    13321 tgtagaaatc tttacagcag ccttggatga acgtttgaac gaacacggtt atatcgttcc
    13381 aggtcttgga gatgctggag accgcttgtt cggtacaaaa taagatcgaa aagaagaatg
    13441 acttggaaaa gtatttctag tcacgaaagg aggttgggtt tttgtttctg tctaatgaaa
    13501 gcagagcaaa aatttgacct tttttgacca agatattata atagtcttat cttcagtcat
    13561 ttgaccaaca aaattaaaac tcaaaaggag aaatgaatga ttcctgtagt tattgaacaa
    13621 acaagccgtg gagaacgttc ttacgatatt tactcacgtc ttctcaaaga ccgcatcatt
    13681 atgctgacag gtccggttga agacaatatg gctaactctg ttattgccca attgcttttc
    13741 ttggatgccc aagatagtac gaaagatatt tacctttatg tcaatacacc aggtggttct
    13801 gtttcagctg gtttggcaat cgtagatacc atgaacttta tcaaggcaga tgtccaaacc
    13861 attgttatgg gaatggctgc atctatgggg actgtcatcg catcaagtgg ggcaaaaggc
    13921 aaacgtttca tgcttccaaa tgctgaatac atgattcacc aaccaatggg cggtacaggt
    13981 ggtggtaccc aacaaactga tatggctatc gctgcagaac acttgctcaa aactcgtaat
    14041 accttggaaa aaatcttggc tgaaaattca ggtcagtcaa tggaaaaagt ccatgcagat
    14101 gcagaacgtg ataactggat gagcgcccag gaaacacttg aatatggctt tattgatgaa
    14161 attatggcca acaattcatt gaactaatga tgatagaagg caaactcgac tgggtttgct
    14221 ttttttggta taatagggag agatttctta gaaagaggat ttatcatgtt tgaaaaagtc
    14281 aatcgctctg gcttgattat ctatctttac tataatcgtg atgccaaaaa actgcaggat
    14341 tatggagata ttacctatca ttccaagaaa catcgttact tacaactcta tgttccaact
    14401 caagaagtgg agcaattggt cggacgcttg agcaaggaaa aatttattaa aaaagttagg
    14461 gtttgtcata tccaagagtt ggaaacaccc tttgtgggca atctttatcg ataggaaaac
    14521 gttatcatcg aaaaagttca aggaaaacat tgacaatttt ctgataattc ggtatattct
    14581 taacagacta tttaagaaat aaggagacaa aaagatgaag aaaaaatttg ccctatcgtt
    14641 tgtggcgctt gcaagtgtag cacttcttgc agcctgtgga gaagtgaagt ctggagcagt
    14701 caacactgct ggtaactcag tagaggaaaa gacaattaaa atcgggttta actttgaaga
    14761 atcaggttct ttagctgcat acggaacagc tgaacaaaaa ggtgcccaat tggctgttga
    14821 tgaaatcaat gccgcaggtg gtatcgatgg aaaacaaatc gaagtagtcg ataaagataa
    14881 taagtctgaa acagctgagg ctgcttcagt tacaactaac cttgtaaccc aatctaaagt
    14941 atcagcagtc gtaggacctg cgacatctgg tgcgactgca gctgcggtag cgaacgctac
    15001 aaaagcaggt gttccattga tctcaccaag tgcgactcaa gatggattga ctaaaggtca
    15061 agattacctc tttattggaa ctttccaaga tagcttccaa ggaaaaatta tctcaaacta
    15121 tgtttctgaa aaattaaatg ctaagaaagt tgttctttac actgacaatg ccagtgacta
    15181 tgctaaaggg attgccaaat ctttccgcga gtcatacaag ggtgaaatcg ttgcagatga
    15241 aactttcgta gcaggtgaca cagacttcca agcagccctt acaaaaatga aagggaaaga
    15301 ctttgatgct atcgttgttc ctggttacta taatgaggct ggtaagattg taaaccaagc
    15361 gcgtggtatg ggaattgaca aaccaatcgt tggtggtgat ggattcaacg gtgaggagtt
    15421 tgtacaacaa gcaactgctg aaaaagcatc aaacatctac tttatctcag gcttctcaac
    15481 tactgtagaa gtttcagcta aagctaaagc cttccttgac gcttaccgtg ctaagtacaa
    15541 tgaagagcct tcaacatttg cagccttggc ttatgattca gttcaccttg tagcaaacgc
    15601 agcaaaaggt gctaaaaatt caggtgaaat caagaataac cttgctaaaa caaaagattt
    15661 tgaaggtgta actggtcaaa caagcttcga tgcagaccac aacacagtca aaactgctta
    15721 catgatgacc atgaacaatg gtaaagttga agcagcagaa gttgtaaaac cataatagaa
    15781 aaatgttgaa atagggaatg agcctttgac tcactccctg tttcgatatt taatactctt
    15841 cgaaaatctc ttcaaactgc gtcaacgtcg ccttggatta tatatgtgac tgacttcgtc
    15901 agtcttatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt tcctagtttg
    15961 ctctttgatt ttcattgagt ataagaacct atcaaaaagt gagggaaacc cctcggaatt
    16021 ataaatagaa agagtgaatc ttatgctcca acaactcgta aatggtttga ttctaggtag
    16081 tgtttacgcg ctgttagccc taggatatac catggtttac ggaattatca agctcatcaa
    16141 cttcgcccat ggtgatattt atatgatggg agcctttatc ggttatttct tgatcaattc
    16201 tttccaaatg aatttctttg tagcgcttat tgtagctatg ctagcgacag ctattcttgg
    16261 tgtcgtgatt gagtttcttg cttaccgacc tttgcgccac tctactcgta ttgctgtttt
    16321 gattacggct attggggttt ctttcctatt ggagtatgga atggtctatc tggttggtgc
    16381 caatacccgt gccttccctc aagcgattca aacagttcga tatgatttgg gaccaattag
    16441 cttaacaaat gtgcagttaa tgattttggg catttccttg attttgatga ttttgttaca
    16501 agtcattgtc caaaagacta agatggggaa agccatgcgt gcagtatcag tagatagcga
    16561 cgcggcgcaa ttgatgggga tcaatgtaaa ccgtacgatt agctttacct tcgctttggg
    16621 ttctgctctt gcgggtgcgg ctggtgttct gattgctctt tattataact ctcttgagcc
    16681 tttgatgggg gttactccag gtcttaaatc tttcgttgcc gcagtacttg gtggtatcgg
    16741 aattattcct ggtgcggctc ttggtggctt tgtgattggt ctattggaaa cctttgcgac
    16801 agcctttggg atgtcagatt tccgtgatgc cattgtttat ggaatcttgt tgttgatctt
    16861 gattgtccgc ccagctggta tccttggtaa gaatgtgaaa gagaaggtgt aaacgatgaa
    16921 ggaaaattta aaagttaata ttctatggtt actccttttg ttagctggct atagcttgat
    16981 tagtgtactg gtttcagtcg gagtacttaa tctattctat gtacagattt tacaacaaat
    17041 tggaattaat attattttgg ctgttggtct caacttaatc gttggttttt caggacaatt
    17101 ttcacttggt catgctggtt tcatggcgat tggtgcctat gccgcagcta ttattggttc
    17161 taaatcacca acctacggtg ccttctttgg agctatgctt gtaggggctt tgctttcagg
    17221 agcagttgcc ttacttgtcg gcattccaac cttgcgcttg aagggggact atcttgcggt
    17281 agcaactctg ggtgtttctg aaattatccg tatctttatc atcaatggtg gaagtcttac
    17341 aaatggtgcg gcaggtatct tagggattcc taactttaca acttggcaaa tggtttactt
    17401 ctttgtcgtg attacaacca ttgcaacctt gaacttcttg cgtagcccaa ttggccgttc
    17461 aaccctctct gttcgtgaag atgaaatcgc tgctgagtca gttggggtta atacgactaa
    17521 aattaaaatc atcgcttttg tctttggtgc cattactgca agtattgctg ggtcacttca
    17581 ggcaggattt atcgggtctg ttgtaccgaa agattacacc ttcatcaact caatcaacgt
    17641 tttgattatt gttgtatttg gtggactcgg ttccattaca ggtgcgattg tttcggctat
    17701 tgttctggga attttgaata tgcttctcca agatgttgct agtgtgcgta tgattattta
    17761 cgctttggcc ttggtattgg taatgatttt cagaccaggt ggactccttg gaacgtggga
    17821 gctgagccta tcacgtttct ttaaaaaatc taagaaggag gaacaaaact aatggcatta
    17881 cttgaagtaa aacagttaac caaacatttt ggtggtctaa cagctgttgg agatgtgact
    17941 cttgaattga acgaagggga actggttgga ttaatcggtc caaacggagc tgggaaaacc
    18001 acccttttca accttttgac cggtgtttat gaaccaagcg agggaacagt aaccctagat
    18061 ggtcaccttt tgaatgggaa atcaccttat aagattgcct ctttgggact tggacgtact
    18121 ttccaaaata tccgtctctt taaagattta acagttttag ataatgtttt gattgctttt
    18181 ggaaaccatc acaaacagca tgtttttact agtttcttac gcttaccagc tttttacaag
    18241 agtgaaaaag aattaaagga taaagctttg gaattgttga aaatctttga tttagatggt
    18301 gatgcagaga ctcttgctaa aaatctttcc tacggacaac aacgtcgttt ggaaattgtt
    18361 cgtgcccttg ctacggaacc taaaattctc ttcttagatg aaccagcagc aggtatgaac
    18421 ccacaggaaa cagccgaatt gactgagtta attcgtcgta tcaaagatga atttaagatt
    18481 acaatcatgt tgattgaaca cgatatgaat ctggtcatgg aagtaacaga acgtatctac
    18541 gtacttgaat atggccgttt aattgctcaa ggaactccag acgaaattaa gaccaataaa
    18601 cgcgttatcg aagcttatct aggaggtgaa gcctaatgtc tatgttaaaa gttgaaaatc
    18661 tttctgtgca ttacggtatg atccaagcag tccgtgatgt aagctttgaa gttaatgaag
    18721 gagaagttgt ttcccttatc ggtgccaacg gtgcaggtaa gacaactatt cttcgcacct
    18781 tgtcaggttt ggttcgacca agttcaggaa agattgaatt tttaggtcaa gaaatccaaa
    18841 aaatgccagc tcagaaaatt gtggcaggtg gtctttcaca agttccagaa ggacgccacg
    18901 tctttcctgg cttgactgtt atggaaaatc ttgaaatggg agctttctta aagaaaaatc
    18961 gtgaagaaaa tcaagctaac ttgaagaagg ttttctcacg ctttcctcgt cttgaagaac
    19021 gtaagaacca agatgcagct actctttcag gaggggaaca acaaatgctt gccatgggac
    19081 gcgctcttat gtcaacacca aaacttcttc ttttagatga accatcaatg ggacttgccc
    19141 caatcttcat ccaagagatt tttgatatca ttcaagatat tcagaagcaa ggaacaaccg
    19201 tcctcttgat tgaacaaaat gccaataaag cacttgcaat ctctgaccga ggatatgtac
    19261 tggaaacagg gaaaatcgtc ctatcaggaa caggaaaaga actcgcctca tcagaagaag
    19321 tcagaaaagc atatctaggt ggctaaaaca atccagtgga ttgttttagt cggcagatga
    19381 agattacgaa gtaatcatca atatagtccg ggggaccttt ttagtcggta aattgagatt
    19441 gcaaacaaat atgcatctac attgaaagct taatttctaa taattgaaaa aatcgaatga
    19501 aaagtttctt accttcattc acagagctcg atttcagagc tctttttgct agcttattca
    19561 tacttttttg aatttcgaaa aagaaatgta agcgtttgat agatttacaa aaagattgta
    19621 taatagggat aagaatagaa aaggagaagt ttcatggcag ttaaagattt tatgacccgc
    19681 aaggtagttt atattagtcc agatacaaca gtatctcatg cagcagattt gatgagagag
    19741 caaggtttgc accgtctgcc tgttatcgaa aatgatcaat tagttggttt ggtgactgag
    19801 ggaaccattg cacaagcaag tccatctaaa gcaacaagtc tttctatcta tgagatgaat
    19861 tatcttctga ataagacaaa agtaaaagat gtcatgattc gcgatgttgt cactgtctca
    19921 ggctatgcta gtctagaaga tgcaacttat ctgatgttga aaaataagat tggtattctc
    19981 cctgtcgtag ataaccatca agtatacgga gttattactg accgtgacgt tttccaagcc
    20041 tttcttgaaa ttgcaggtta tggcgaagaa gggattcgtg tacgctttgt tacagaagat
    20101 gaagttggtg ttcttggaaa aattgtttct ttgattgtag aagaaaattt gaatatctcc
    20161 catacagtca atattccgcg taaggatggt aaggtgatta tcgaagtgca aatcgatgga
    20221 tcaattgatt taccagcctt gaaagaaaaa tttgaagcaa atggtattca agtggaagaa
    20281 atcgctcgca cttcagcaaa agtcttgtaa gaagggaagc ccaaaggctt cttttttcat
    20341 gaaaagggga ttagagcaaa agatggaaag aaatgataaa atatgctata atgaaataat
    20401 gtaaaaaagg agtatttatg gacatttcag taattcgtca gaaaattgac gcaaatcgtg
    20461 aaaaattagc ttctttcagg gggtctcttt gacctcgaag ggctagagga agagattgcc
    20521 atcttggaaa acaagatgac agaacctgat ttttggaacg ataatattgc ggcccaaaaa
    20581 acgtcgcaag aattgaatga attaaaaaac acttacaata ccttccataa gatggaagag
    20641 ttgcaggatg aagtcgaaat tttattggat tttttggctg aagacgagtc agtgcatgat
    20701 gaactggtag cgcagttagc cgaacttgat aagataatga ccagctacga gatgactcta
    20761 ctcttgtcag aaccttatga ccacaacaat gccatcttgg aaatccatcc aggttctggt
    20821 ggtactgagg cgcaggactg gggtgatatg ttgcttcgta tgtatactcg ttatggtaat
    20881 gctaaaggct ttaaagtgga agtgttggat taccaagcag gtgatgaggc tggtattaag
    20941 tcggtaactt tatcatttga agggcctaat gcctatggtc tcctcaagtc agaaatgggt
    21001 gttcaccgct tagtgcgaat ctcaccattt gactctgcca aacgtcgcca tacctctttc
    21061 acatctgtag aagtgatgcc agaattggat gatactattg aagtggaaat ccgtgaagat
    21121 gatatcaaga tggatacctt ccgttcaggt ggtgccggtg gacaaaacgt caataaggtt
    21181 tcaacaggtg tacgtttaac ccacattcca actggaattg ttgtccaatc aacagtggat
    21241 cgtacccagt atggaaatag agatcgtgcc atgaagatgt tgcaggctaa gctctatcaa
    21301 atggagcaag ataagaaggc tgcggaagta gattctctca aaggtgagaa aaaggagatc
    21361 acttggggaa gccaaatccg ttcttatgtc ttcacgcctt atactatggt aaaagatcac
    21421 cgaactagct ttgaggttgc tcaggtagat aaggttatgg atggggacct agatggtttt
    21481 atcgatgctt atctcaagtg gcgaattagc taagatagaa aggaactcac atgtcaatta
    21541 ttgaaatgag agatgtcgtt aaaaaatacg acaacggaac aactgctcta cgcggtgttt
    21601 cggttagcgt tcaaccgggg gaatttgctt acatcgtagg accttcagga gcagggaagt
    21661 caacttttat tcgttctctg tatcgtgaag taaaaatcga taaaggaagc ctatcagttg
    21721 ctggttttaa tctggttaag atcaaaaaga aagatgtccc gcttctacgt cgtagtgttg
    21781 gggttgtctt ccaggattat aaattgttac caaagaaaac tgtctatgaa aatattgctt
    21841 acgctatgga agtaatcggg gaaaatcgcc gtaatatcaa aagacgagtg atggaagttt
    21901 tggacttggt tggattgaag cataaggttc gttctttccc aaatgaactc tcaggtgggg
    21961 agcaacagcg gattgcgatt gcgcgtgcaa ttgtaaataa tcccaaagta ttgatagctg
    22021 atgagccaac aggaaatctg gatccggata attcatggga aattatgaat ctcttggaac
    22081 ggattaacct acaaggaaca actattttga tggcgactca taatagccag attgtaaata
    22141 ccttgcgcca ccgtgtcatt gccattgaaa atggccgtgt cgttcgtgac gaatcaaaag
    22201 gagagtatgg atacgatgat tagtagattt tttcgccatt tatttgaagc cttaaaaagt
    22261 ttgaaacgaa atggttggat gacagtagct gctgtcagtt cagtcatgat tactttgacc
    22321 ttggtggcaa tatttgcatc tgttattttc aatacagcga aactagctac agatattgaa
    22381 aataatgtcc gtgtagtagt ttatatccga aaggatgtgg aagataatag tcagacaatt
    22441 gaaaaagaag gtcaaactgt tacaaataat gactaccaca aggtatatga ttctttgaag
    22501 aacatgtcta cggttaaaag tgttaccttt tcaagtaaag aagaacaata tgaaaaatta
    22561 accgagataa tgggagataa ctggaaaatc tttgaaggag atgccaatcc tctctatgat
    22621 gcctatattg tagaggcaaa cactccaaat gatgtaaaaa ctatagccga agatgctaaa
    22681 aaaattgaag gtgtctctga ggttcaagat ggcggtgcca atacagaaag actcttcaag
    22741 ttagcttcat ttatccgtgt ttggggacta gggattgctg ctttgttaat ttttatcgca
    22801 gtttttttga tttcaaatac cattcgtatt accattattt cccgcagtcg cgaaattcaa
    22861 atcatgcgct tggtcggagc taaaaacagt tatatccgtg gaccgttctt gttagaagga
    22921 gcctttatcg gtttattggg agctatcgca ccatctgttt tggtctttat tgtttatcaa
    22981 attgtttatc aatctgtcaa caaatcgttg gtagggcaaa atctatccat gattagtcca
    23041 gatttattta gtccgttgat gattgcccta ctatttgtga ttggggtttt cattggttca
    23101 ttgggatcag gaatatccat gcgccgattc ttgaagattt aggtaaaata gctgctttta
    23161 tgaggagatt gtaaaatctc cttttttgct acaagagttt ttgaaaagag atgcgcagaa
    23221 gaaaagagct tccaaagaag tcccccagag aagacttctt gatggctaat ttgaacttga
    23281 aatttgactc actagaaatt tttcccaaat ttcaagttga agttagccac ccaaaagaaa
    23341 ttcacttggc gacttgtagt ccaagcattt tttagggtag ttgttaatcc aattttcgat
    23401 aaaagctact tctttcggag tcgttttctt ggttccttta ggtaaccatc tccgaattaa
    23461 tcgattatga ttttcatttg aacctctctc ccatgaagag taagcatgtg cgtagtagat
    23521 atgttcctca ggaaatacct cagacaatcg tttgaactcc gaaccattat ctgcagtaat
    23581 ggacagaata cgatgctccc ctaatagtaa cgtaagcgcc tgattgacag attcagcagt
    23641 tttatttgga attaacctta taatttggtg tctgctccgc cggtcggtta agactaacag
    23701 gcaataattt tttatcttag ttagtaggac ggtatcaatt tcataatgac cattttccaa
    23761 gcgaagatta ataacgtcag gacgttcttc gatagattta cctgccggct taaagttcgg
    23821 actagcttgc ttcttgacac ctttcctttt tctgggataa agcatgtcgg ccttcgtcaa
    23881 tcctaaatga ccattatgaa accagtagta gatggttgaa ataccaactt tcacttgctt
    23941 cttgttaacc atcatttcag gcgaaaattt ttgcttatga tagtgtaaga tcttctctct
    24001 gatttccttt gttaaaatta actttttcac cgaccgtttt cgattgaatt ggtaaacagt
    24061 ttgtgcgtaa tcggcagaat agaccttttt gtatagccct tttctcactt gttgtaaagt
    24121 tgtacctctt ttgacttcag tatgaatcgt ttgaggcgcc tttcctaaca agcctgcaat
    24181 ttcacgattg gacttatttt cattcttcca ccgctcaatc aagcgacggt tatctattgt
    24241 caaatgtttc ccttttgggg tataatgttc ttgcatctct gtgcctttcc ttgtgtttgt
    24301 ggtgaacaac aagtatagca cagaggtgtt ttcttatacc ttaaatcaca tttcatttga
    24361 caacaggaac ccttgaacct gttttttcag ctaaaccaag gctatgatta gccttggcca
    24421 cctagcctaa cagtttcaaa cctgttatca aatgaaatca tcaacttact caaacatagt
    24481 gaaacatcta attcttgact aaggtaaggt ggctaacttc attatagaac tttcaaattt
    24541 tctaattttt aatcttgaaa taggacaacg atggtcttca tagaaaacaa tttctaagtt
    24601 ttttcgatca atttctctga tattgcctat atttaccaaa aatgacttat gaggagaata
    24661 aaatcgctga gtatgtttgt ccttttcctg aatatctgtc atggtaccat aaaactcttt
    24721 tgcaaaattc ttaccaataa tgcgcaattt atgagagacc cctgttgttt caatatacaa
    24781 aatatcatgg taaggaattt ttaaatcatt tcccttgtaa ttgtagtcga aataatctac
    24841 aacatcttca ttttcaagta acatactctt cgtgtagaag atattttgct caattctctt
    24901 cttaaacatc tcatcattga tatccttatc aacaaaatct agggctgata cctggtattt
    24961 ataggttaga gtcgcaaact ctgatcgact agtgataaag acgataatag cgtaaggatt
    25021 gtaatgacga atgagctgag ccacttcaaa tccctttttc tcaattccat gaatatcgat
    25081 atctaggaaa taaagctgat ttacttcatc attttcaatg tattcttcaa attcacggac
    25141 ttttcccgtt gtcttgtatg atattggaat attcgattct ttcgaaattt catccaatat
    25201 tctctctagt ctcacttgat gttcaataac atcttctaaa attaaaactt tcattcaaat
    25261 tccctcttaa atctaatgat ttgtctaaat gtactgcctt ccatctctgt ttctaaaata
    25321 atattgttgt acttatctag tagttctttc acattattta atccgactcc gcgatttctt
    25381 cccttagtgg agaatcctaa ggcaaataga tctcctgaag gagtcatcgt cattttacat
    25441 gaattctgaa tcacaataac tgtttcagtt tccatcttaa taactgctac ttccatctgc
    25501 tttttatagc tatcagccga tccttcgaca gcattattca ataaaacgct catgatacga
    25561 accaaatcca atagttcaat tggaagcttg gtaatcgtat cttttacttc cagtgtaaac
    25621 tctacaccat tatttcgagc atagacaatt gactgagcaa ccaaacttcg taaagctgag
    25681 tcttctatgt tgttcaaatc aaagtaagtg tacttatctg aacgcaattt atgatttgct
    25741 ttgactaaaa cttcattgta aattctgtca atttcctgta aattaccact gtcaattgcc
    25801 atctgcatgc tgacaagcat tccagcataa tcatgtcgaa aaccacggat ttcattatac
    25861 agaccaacaa tttcatctgt gtaattctgt aaatgtttct gttcaaattt cttctgcttc
    25921 aaagcaatct ctttctccat ttgaacttta tgagaattca ttgcaaagaa ggtcaaaagg
    25981 agagagataa agacaataga tgacaaaata cttccaaaac tattcaaatg tttaatcgta
    26041 cttaccatat ctgaaacgaa agatacaata tgtagcaata gtaaagcaaa aaatactttt
    26101 ttcaagaaag gataaaggta gtcctcgtca aaataggcta gttccaaatg gaaatagtaa
    26161 atgattttta atgtaacaaa ataggttaac accgtcacaa cgaaaaagaa tgggaaatga
    26221 tattgtaaaa caaaattatc tcctgttata gaggagaaag ttacggacag aaagttatga
    26281 gtgctctcat ataaaagaga tagtagtaaa cttaggaata gtcctctatc cctctcatac
    26341 tgtttcatcc atcgaaaata ggaatataat cccaaaggaa ataaaaatct ttca
//
LOCUS       DAVMAF010000022        26185 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMAF010000022 DAVMAF010000000
VERSION     DAVMAF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.022"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            191..1462
                     /locus_tag="VYO97_000925"
     CDS             191..1462
                     /locus_tag="VYO97_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1753744.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1573..2865
                     /locus_tag="VYO97_000926"
     CDS             1573..2865
                     /locus_tag="VYO97_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1753745.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2867..3709
                     /locus_tag="VYO97_000927"
     CDS             2867..3709
                     /locus_tag="VYO97_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1753746.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3962..4762
                     /locus_tag="VYO97_000928"
     CDS             3962..4762
                     /locus_tag="VYO97_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1753747.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4772..5758
                     /locus_tag="VYO97_000929"
     CDS             4772..5758
                     /locus_tag="VYO97_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1753748.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNGNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5894..5974
                     /locus_tag="VYO97_000930"
                     /pseudo
     CDS             5894..5974
                     /locus_tag="VYO97_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            6008..6091
                     /locus_tag="VYO97_000931"
                     /pseudo
     CDS             6008..6091
                     /locus_tag="VYO97_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(6095..7036)
                     /locus_tag="VYO97_000932"
     CDS             complement(6095..7036)
                     /locus_tag="VYO97_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1753749.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(7014..8777)
                     /gene="aspS"
                     /locus_tag="VYO97_000933"
     CDS             complement(7014..8777)
                     /gene="aspS"
                     /locus_tag="VYO97_000933"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1753750.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(9012..9092)
                     /locus_tag="VYO97_000934"
                     /pseudo
     CDS             complement(9012..9092)
                     /locus_tag="VYO97_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9128..9358)
                     /locus_tag="VYO97_000935"
     CDS             complement(9128..9358)
                     /locus_tag="VYO97_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753751.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(9376..10017)
                     /locus_tag="VYO97_000936"
     CDS             complement(9376..10017)
                     /locus_tag="VYO97_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1753752.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(10029..10709)
                     /locus_tag="VYO97_000937"
     CDS             complement(10029..10709)
                     /locus_tag="VYO97_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1753753.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            complement(10765..10962)
                     /locus_tag="VYO97_000938"
     CDS             complement(10765..10962)
                     /locus_tag="VYO97_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753754.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(10962..11168)
                     /locus_tag="VYO97_000939"
     CDS             complement(10962..11168)
                     /locus_tag="VYO97_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1753755.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(11178..11492)
                     /locus_tag="VYO97_000940"
     CDS             complement(11178..11492)
                     /locus_tag="VYO97_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753756.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(11759..11836)
                     /locus_tag="VYO97_000941"
                     /pseudo
     CDS             complement(11759..11836)
                     /locus_tag="VYO97_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(11833..13122)
                     /locus_tag="VYO97_000942"
     CDS             complement(11833..13122)
                     /locus_tag="VYO97_000942"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1753757.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISKNFSEIFEKLGFYTQ"
     gene            complement(13200..14447)
                     /locus_tag="VYO97_000943"
     CDS             complement(13200..14447)
                     /locus_tag="VYO97_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1753758.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFVASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGIW
                     FLALSVNNFSLVVGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLFINSLGGALGGIYNIFFLSHPLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFL
                     TLYVSGKVSPKISTMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGISLLAIILTGLNLKNDI"
     gene            complement(14640..15491)
                     /gene="rgg"
                     /locus_tag="VYO97_000944"
     CDS             complement(14640..15491)
                     /gene="rgg"
                     /locus_tag="VYO97_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1753759.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSAFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            15615..15956
                     /locus_tag="VYO97_000945"
     CDS             15615..15956
                     /locus_tag="VYO97_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1753760.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            16014..16094
                     /locus_tag="VYO97_000946"
                     /pseudo
     CDS             16014..16094
                     /locus_tag="VYO97_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(16210..17913)
                     /gene="ilvD"
                     /locus_tag="VYO97_000947"
     CDS             complement(16210..17913)
                     /gene="ilvD"
                     /locus_tag="VYO97_000947"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1753761.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(18146..19078)
                     /locus_tag="VYO97_000948"
     CDS             complement(18146..19078)
                     /locus_tag="VYO97_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1753762.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDVVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTVFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(19075..19932)
                     /locus_tag="VYO97_000949"
     CDS             complement(19075..19932)
                     /locus_tag="VYO97_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1753763.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNSGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(19936..21282)
                     /locus_tag="VYO97_000950"
     CDS             complement(19936..21282)
                     /locus_tag="VYO97_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1753764.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(21295..21579)
                     /locus_tag="VYO97_000951"
     CDS             complement(21295..21579)
                     /locus_tag="VYO97_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1753765.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELIIKICEEKGLI"
     gene            complement(21583..23613)
                     /locus_tag="VYO97_000952"
     CDS             complement(21583..23613)
                     /locus_tag="VYO97_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1753766.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            23804..24811
                     /locus_tag="VYO97_000953"
     CDS             23804..24811
                     /locus_tag="VYO97_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1753767.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAALTHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNGNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            24814..25014
                     /locus_tag="VYO97_000954"
     CDS             24814..25014
                     /locus_tag="VYO97_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1753768.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            25166..25348
                     /gene="rpmF"
                     /locus_tag="VYO97_000955"
     CDS             25166..25348
                     /gene="rpmF"
                     /locus_tag="VYO97_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1753769.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            25364..25513
                     /gene="rpmG"
                     /locus_tag="VYO97_000956"
     CDS             25364..25513
                     /gene="rpmG"
                     /locus_tag="VYO97_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1753770.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(26005..>26185)
                     /locus_tag="VYO97_000957"
     CDS             complement(26005..>26185)
                     /locus_tag="VYO97_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753771.1"
                     /translation="TGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTT
                     TPDGYRVNANGEWVG"
BASE COUNT         8187 a         5680 c         4606 g         7712 t
ORIGIN      
        1 cttatctatt tcatactttt cttctatata aaatgcaaaa ttatgctatc aaatcaagct
       61 taaaaatttt ttaaaatttt tacaaaaaat acttgcaacc gttttctatt tgtgctatac
      121 taagctcata aaggaaaacg tttgcgtttc cttatcaata aaacttgcta ttctttggga
      181 ggaatacact atgtcatcta aatttatgaa gagcactgcg gtgcttggaa ctgctacact
      241 tgctagcttg cttttggtag cttgcggaag caaaactgct gataagcctg ctgattctgg
      301 ttcatctgaa gtcaaagaac tcactgtata tgtagacgag ggatataaga gctatattga
      361 agaggttgct aaagcttatg aaaaagaagc tggagtaaaa gtcactctta aaactggtga
      421 tgctctagga ggtcttgata aactttctct tgacaaccaa tctggtaatg tccctgatgt
      481 tatgatggct ccatacgacc gtgtaggtag ccttggttct gacggacaac tttcagaagt
      541 gaaattgagc gatggtgcta aaacagacga cacaactaaa tctcttgtaa cagctgctaa
      601 tggtaaagtt tacggtgctc ctgccgttat cgagtcactt gttatgtact acaacaaaga
      661 cttggtgaaa gatgctccaa aaacatttgc tgacttggaa aaccttgcta aagatagcaa
      721 atacgcattc gctggtgaag atggtaaaac tactgccttc ctagctgact ggacaaactt
      781 ctactataca tatggacttc ttgccggtaa cggtgcttac gtctttggcc aaaacggtaa
      841 agacgctaaa gacatcggtc ttgcaaacga cggttctatc gcaggtatca actacgctaa
      901 atcttggtac gaaaaatggc ctaaaggtat gcaagataca gaaggtgctg gaaacttaat
      961 ccaaactcaa ttccaagaag gtaaaacagc tgctatcatc gacggacctt ggaaagctca
     1021 agcctttaaa gatgctaaag taaactacgg agttgcaact atcccaactc ttccaaatgg
     1081 aaaagaatat gctgcattcg gtggtggtaa agcttgggtc attcctcaag ccgttaagaa
     1141 ccttgaagct tctcaaaaat ttgtagactt ccttgttgca actgaacaac aaaaagtatt
     1201 atatgataag actaacgaaa tcccagctaa tactgaggct cgttcatacg ctgaaggtaa
     1261 aaacgatgag ttgacaacag ctgttatcaa acagttcaag aacactcaac cactgccaaa
     1321 catctctcaa atgtctgcag tttgggatcc agcgaaaaat atgctctttg atgctgtaag
     1381 tggtcaaaaa gatgctaaaa cagctgctaa cgatgctgta acattgatca aagaaacaat
     1441 caaacaaaaa tttggtgaat aaaaaatttg ttcaaggggg gtggaaatca aatccccctt
     1501 tgaatttatc aatagagaca caaataattt agctttctta taaaaaagta gtatcctatg
     1561 aaaggagtta atatggaaaa gcaacaacct agtaaagcag ccctgctgtc tatcattcct
     1621 gggttaggac agatttacaa taaacaaaaa gccaaaggtt ttatcttcct tggtgtaacc
     1681 atcgtatttg tcctttactt cctagcactt gcaacccctg aattgagcaa cctcatcact
     1741 cttggtgaca aaccaggtcg tgataattcc ctctttatgc tgattcgtgg tgccttccat
     1801 ctaatctttg taatcgttta tgtactcttt tatttctcaa atatcaaaga tgcacatacg
     1861 attgcaaaac gcattaacaa tggaattcca gttccacgca cactcaaaga catgatcaaa
     1921 gggatttatg aaaatggctt cccttacctc ttgatcattc catcttatgt tgccatgacc
     1981 ttcgcgatta tcttcccagt tatcgtaacc ttgatgatcg cctttaccaa ctacgacttc
     2041 caacacttgc caccaaacaa gttgttggac tgggttggtt tgaccaactt tacaaacatt
     2101 tggagcttga gtaccttccg ttctgccttt ggttctgttc tttcttggac tatcatttgg
     2161 gctttggcag cttctacttt acaaatcgta attggtatct tcacagctat cattgccaac
     2221 caaccattta tcaaaggaaa acgtatcttt ggtgttattt tccttcttcc ttgggctgtc
     2281 ccagccttca tcactatctt gacattctca aacatgttta acgatagtgt cggtgctatc
     2341 aacactcaag tattgccaat cttggctaaa ttccttcctt tccttgatgg agctcttatt
     2401 ccttggaaaa cagacccaac ttggactaag attgccttga ttatgatgca aggttggctc
     2461 ggattcccat acatctacgt tttgactttg ggtatcttgc aatctattcc taacgacctt
     2521 tacgaagcag cttatattga cggtgccaac gcttggcaaa aattccgcaa catcactttc
     2581 ccaatgattt tggctgttgc ggcacctact ttgattagcc aatacacctt caactttaac
     2641 aacttctcta tcatgtacct cttcaatggt ggaggacctg gtagtgtcgg aggtggagct
     2701 ggttcaaccg atatcttgat ctcatggatc taccgtttga caacaggtac atctcctcaa
     2761 tactcaatgg cggcagctgt taccttgatt atctctatca ttgtcatctc aatctctatg
     2821 atcgcattca agaaactaca cgcatttgat atggaggacg tctaagatga ataactcaat
     2881 taaactcaaa cgtagactga ctcaaagcct tacttacctt tacctgattg gtctatcaat
     2941 tgtaattatc tatccactgt tgattaccat tatgtcagcc tttaaagcag gtaacgtctc
     3001 agcctttaaa ctagatacta atatcgacct caattttgat aactttaaag gcctcttcac
     3061 tgaaaccttg tacggtactt ggtacctcaa cactttgatt atcgccttaa ttaccatggc
     3121 tgttcaaaca agtatcatcg tacttgctgg ttatgcttac agccgttaca acttcttggc
     3181 tcgtaaacaa agtttggtct tcttcttgat catccaaatg gtgccaacta tggccgcttt
     3241 gacagccttc ttcgttatgg cgcttatgtt gaacgccctt aaccacaact ggttcctcat
     3301 cttcctctac gttggtggtg gtatcccgat gaatgcttgg ctcatgaaag gctacttcga
     3361 tacagtgcca atgtctttag acgaatctgc aaaactagac ggtgcaggac acttccgccg
     3421 cttctggcaa attgttctac cacttgttcg cccaatggtt gccgtacaag ctctctgggc
     3481 cttcatggga cctttcgggg actacatcct ctctagtttc ttgcttcgtg agaaagaata
     3541 ctttactgtt gccgtaggtc tccaaacctt cgttaacaat gcgaaaaact tgaagattgc
     3601 ctacttctca gcaggtgcta tcctcatcgc ccttccaatc tgtattctct tcttcttcct
     3661 acaaaagaac tttgtttcag gacttacaag tggtggcgac aagggataat ttatccccgc
     3721 cacccttttt cattttatac tcttcgaaaa tctcttcaaa ccacgtcagc tttatctcca
     3781 acctcaaagt tgtgctttga gcaacctgtg gctagtttgc actttgattt tcattgatta
     3841 ttagcaattg tcactgtaaa taatatcctt gtagcaagca atttttctcc tagacttgaa
     3901 ataaagcgca tttctctata taataatact catatagaaa acacctttta gaaagatacc
     3961 tatgcttcca tatccatttt cctatttttc aagtatttgg gggtttcgta agcccctgtc
     4021 caaacgtttc gagctcaact ggtttcaact tctctttacc agtatcttcc ttatcagctt
     4081 gtctatggta cccattgcta tccaaaacag ctcccaggag acctatccgc tagaaacttt
     4141 tatcgataat gtctatgaac ctctgacaaa tgaggttgtc caggatctct ctgaacatgc
     4201 tacaattgtc gatggcacat taacttatac tggaacagct agtcaaaccc cttctgttgt
     4261 gattggtcca agtcaaatca aggaattacc taaggacttg caactgcatt tcgatacaaa
     4321 tgagctagtc atcagcaagg aaagcaagga gctgacccgc atctcttacc gagccattca
     4381 gactgagagt ttcaaaagca aagacagctt gacccaagca atttctaaag actggtacca
     4441 acaaaatcgt gtctatatca gcctcttcct agttctcggt gcgagcttcc tctttggttt
     4501 gaatttcttt atcgtctctc taggagctag tcttctcctt tatatcacta agaaatcacg
     4561 cctcttttca tttagaacct ttaaagagtg ctatcacttt atcttgaact gtttaggatt
     4621 gccgactctg attacactta ttttgggatt atttggccaa aatatgacaa ccctgattac
     4681 tgtacaaaat attctttttg ttctgtatct ggtcactatc ttttataaaa cacatttccg
     4741 tgatccaaat taccataaat aggagatttt tatgcccgtt acgattaaag acgtggccaa
     4801 ggctgctggt gtttcgcctt caaccgtaac ccgtgttatt caaaataaat caaccattag
     4861 cgacgaaaca aaaaaacgcg ttcgcaaagc tatgaaggaa ctcaactacc acccaaacct
     4921 caacgctcgt agcttggtaa gcagctatac tcaggttatc ggattagttc ttcctgatga
     4981 ctcagacgcc ttctaccaga atcctttctt tccatcggtt ctacgtggca tctctcaagt
     5041 cgcatctgaa aaccactatg ccattcagat agcaacaggg aaagatgaga aggagcgtct
     5101 caacgctatt tcacaaatgg tctacggcaa gcgtgtagat gggctaatct ttctctatgc
     5161 ccaagaagaa gaccctctcg taaaactcgt cgcagaagaa cagttcccct tccttatctt
     5221 aggtaaatct ctatctcctt tcatcccact tgtcgacaac ggcaatgttc aagctggttt
     5281 tgatgcgact gaatatttca tcaaaaaagg ctgcaaacgc attgccttta tcggaggaag
     5341 taaaaagctc ttcgtgacca aagaccgttt aacaggctat gaacaggcgc ttaaacatta
     5401 caaacttacc actgacaaca atcgcatcta ctttgccgac gagtttctgg aagaaaaggg
     5461 ctataaattt agcaagcgat tattcaagca cgatccacaa attgatgcta tcatcacaac
     5521 cgatagcctc ctagctgaag gtgtttgtaa ctatattgcc aaacaccagc tggatgtccc
     5581 tgttctcagc tttgactcgg ttaatcccaa gctcaacttg gcagcctatg tcgatatcaa
     5641 tagtttagag cttggtcgtg tttcccttga aactattctc cagattatta atgataataa
     5701 aaacaataaa caaatttgtt accgtcaatt gatcgcccac aaaattatcg aaaaataaga
     5761 gactgggcaa aaagtcgtta aaagcaaaaa cgcatactat caggtattga aaaaacttga
     5821 tactatgcgt tttaatgtgg gaagatttac ttccttttct actgaaattg agtcttttcc
     5881 caagatcttt ttatactcaa tgaaaatcaa agtgcaaact aggaagctag ccgcaggttg
     5941 ctcaaaacac tgttttgagg ttgtagatga aactgacgaa gtcagctcaa aacatggttt
     6001 tgaggttgta gatgaaactg acgaagtcag taaccatacc tacggcaagg tgaagctgac
     6061 gtggtttgaa gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc
     6121 ctagaatatg aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa
     6181 tctgtttaaa ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct
     6241 cgtgattata ggttccttct gcatcgtgga tcatggttgc tccgcggtgc aattttttat
     6301 ggattttttc aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt
     6361 gcttagtaaa gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa
     6421 gagcgtattt ccaaccaaag gttaaacctg ctatcagcat gatagttcca tttaccaaga
     6481 aagaaatact accgacattc ttacccgttt tcttgcgaat agtcaggctg acgatatccg
     6541 tcccaccact ggagatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac
     6601 ccccaaaaag ggaattgata atgggatcct ccgtcaaggt tgccacaggg acaaactgga
     6661 taaagaagga actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct
     6721 gataccaagc taagaccatc aaagggaagt taatggcgta gaagcttagc gaaatcggaa
     6781 tatgaaaacc aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac
     6841 cactcgaata cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac
     6901 catagaccag agaggccgaa atcttctcat catacttttc tcgagagata ctttgtaaga
     6961 cacgtaaaat ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc
     7021 gttttgcttg tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg
     7081 actgttgatg gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg
     7141 acttcacgga tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc
     7201 aaaccaccgt gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca
     7261 ttggcttctt cagttgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg
     7321 ttgatacgaa ggctaccacc accaagctca taaccgttca agacgatatc ataagcaatg
     7381 gcacgaacct tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagggtg
     7441 aaaggatggt gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag
     7501 tcaaccaccc aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata
     7561 cgtccacgaa gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga
     7621 accaagtcct tatcttcaag aacaagcgct gttgtcaatt cttcttggat accagtcaag
     7681 aacttggcaa ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg
     7741 gcaccatact gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc
     7801 gcagctcctg tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct
     7861 acacctcgga ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg
     7921 tcagaaccgt aaagagccat agcatcatcg tatttcatac gagggaatgg tagcgttact
     7981 tcgatgcctt ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt
     8041 tcttgctcag taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct
     8101 ccacgcaagt cctcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca
     8161 ttcatcaaga gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta
     8221 ttaacacgag acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat
     8281 ggtgtctcca cgtcgataaa ctccaactca tccaagtagt tgcggataga gtgggtcacc
     8341 ttggcacgaa gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa
     8401 cgcaaacgtg tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta
     8461 gctgtgttaa gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta
     8521 tcattggctt gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga
     8581 aggctttcag ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc
     8641 atgattcctt cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg
     8701 ccaacccatc ctttcaaggt tatttcttgt ccgatgtgtt cctcacgaac acgaccagca
     8761 tacatactac gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag
     8821 cgcaggctct tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt caggtgaaag
     8881 ccctaaaaat tagcgctaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt
     8941 accgtagtac ggttactgac ttcgtcagtt tcatctacaa tctcaaaacc atgttttgag
     9001 ctgacttcgt cagtttcatc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc
     9061 ttcctagttt gctctttgat tttcattgag tataatacaa aaatccgatg aacttcaccg
     9121 gactctttta ttttgaattt ttgcctgctt tacgcttttc agcgatttcg gctgcctttc
     9181 gaggcaagac aatttccgtt atgtaagccg tcccaaaacg cagtacacct gcaataggag
     9241 caaagacaac tgctagatag ttatagaaga aatcgccttt gaaggcataa gctagcgctc
     9301 caatgatgaa aaatagaacg actgcctgaa tcactgccaa taaaattact cgtttcatgt
     9361 gacctcctga ctctattata gcatgagaat catcaaaaag ccgactaaat tattcaaagc
     9421 gtgaagagaa atactgtaga ccagaccttt tctgctaatg taagccaaac ccaaactaaa
     9481 accaaggcta aaatagacaa aaaattgttg cacatcacct ggaaaatgaa tcaaggcaaa
     9541 tagaagacta gataccagaa gaaaaatcag ggttcgttta ctattgtcct gcttaggaaa
     9601 gagatagcgt gctaacatcc ctctaaaaac aatctcttcc gtcaaaggag caaaaataac
     9661 cacagcaaag aatgagaaaa gtggttgaga caaggtcaag tctgtcgcta tttgctgatt
     9721 tactgaagga tcatctggca agaagaattg aacgaccaga gataagaacc aaaccaagac
     9781 aggaagccaa ataaatcgat taaagccgct cttctcaata tgaacaggag ccttctgata
     9841 ccatttgtaa atgccgtaca catatactcc agccaaggcc acatagagta gagtaacagc
     9901 atagggtgaa gcgcctaaag caagcgacgc agtcgcgagc ccctgaataa agccatagat
     9961 aaataaaaag gatagaaggg ctagaagaat ccagccaagg tttttaagta atttcataga
    10021 taactccttt atttgaaata acgttttacc ataggtaact gcatcacatt gatataaaca
    10081 tggatggctc ctacaagcaa gaaagctagt aactgaatct ctcctgtcaa gaaagaaatg
    10141 ataataagaa aaatatataa ggctggtaag acatattggt gtaattggaa taaaattcga
    10201 aaactctgtt ccaaattagc ctgacgctcc ccttcatcat aagaatttat atagttcaag
    10261 acatcctttg gtgtagcgaa aaattccaaa tcaaactgac gaacaatcgc aatggtttta
    10321 aaaagagatt tttgagcgac taagaatacc acaaagagta agaaagaaag gaaaaatgtt
    10381 tgagggtttg tatgcaatat aatcacctca cttaatgaaa taaaaatagc caatggaatc
    10441 gctacacctg taatattaaa agcaatggtt ccaaactcaa gattccgata catttgcaca
    10501 taataggttt cattcagatc gtcatccatt tcctcttgat acaaggaatg aaattttctg
    10561 cttttcttta agaaattgaa agtcaaaaac atactaatga aacctatcag taaacaaata
    10621 gctgatatcc atggcatcaa ggcttttaca tctaaaataa tttcgtggga ttcgacacgt
    10681 gccttaaaca tccctacaaa catgcccaag aaccccccaa gacaatagac atcaaaaata
    10741 acaatctacg tttctttttc atattcattc tcctttttca cttgctagat ttttggattt
    10801 cttttcaatc cattcaatta ctgggatgag agcaaagtag acccaaacaa attggtcgct
    10861 ttgataggga ttaaaccagc ttaggtccat cccaatcagt agaaatacgc tgactaataa
    10921 agctatgacc actacataat aaatcacttt atacttgttc atcactcgtc ctcctccaaa
    10981 cgaaataccg attcgactgt ttcgttgaaa atttgagata ttttcagggc aatgataatg
    11041 gatggggtgt actcatcccg ttctagtagg ctaatggtct gtctggaaac ccctgccagt
    11101 ttggctaggt cggtttgatt gagaccatcg cgagctcgaa gctcttttag acgatttttt
    11161 agttgcacgt tacacaccta ctctccgtca aattcaacgg tttggatatc ttcaatacgt
    11221 tgcaacttga atttttcttt tcccgtatta tctacacgtc gtagctttac ccattcctca
    11281 tcaacatcca caacttccca gttatctggc ccaatataca ctcccgttat aattggttcc
    11341 tttccaatca tttcttgtaa taatctcgac atttctgcgt ttcctttctc ttttcgctca
    11401 agtcttttga ttttattctc tagtttcttg atttttttag aattattaga ataaaagaaa
    11461 atcataaata gtataaatac tagtacccac attataactc ctttctgctt cctatttctt
    11521 aacttgaatt cattgtaaca catctttttc tttttgacaa ttatagttgt caaaaagttt
    11581 atgatttttg tcattttgca aaagaaaaag gtcaggagta agttcctgac cactttatct
    11641 atcattaata ctcttctaaa atctcttcaa accacgtcag cttcaccttg ccgtaggtat
    11701 ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcat
    11761 cagttctatc cataacttca aaacactgtt ttgagcaacc tgcggctagc ttcctagttt
    11821 gctctttgat tttcattgag tataaaatcc tagtttttca aagatttctg agaaattttt
    11881 gctgattgtc tcaagtgaca cttgcacttc ttctcgggtt tggttgttct tgaccgtcac
    11941 ttgtccgctt tcgacttcac tctctcctag ggtgatgagg gtcttagcca caaagacatc
    12001 ggctgacttg aactgagctt tgagcttacg gttgaggtaa tcacgctctg ctttgaaacc
    12061 ttgttggcga agagcttgga ctagttctag ggctttgaca tttgctccat cacccaagac
    12121 tgcgatatag acatctaggg cattttcgat agggagggcc acgccttgtt tttcaaggat
    12181 gagaagcagg cgctctacac caagcccaaa accaaatcca gcagtttcag ggcctccaaa
    12241 gtaagcaacc aaaccatcgt agcgaccacc cgcacagact gtcaagtcat tgccctcaat
    12301 ctctgtgata aactcgaaaa tggtgtggtt atagtagtcc agaccacgca ccatattggt
    12361 atcgatgatg taatctactc caagattttc caacatctga cgcacagcat caaaatgagc
    12421 ttggctttct tcatcaagaa agtccaagat agacggcgca ttctctactg ccaccttgtc
    12481 ttctttttcc ttagagtcca agacacgaag aggattttcc tccaagcgac gttggctatc
    12541 cttagacaag gtctccttga gcggtgtcaa atagtcaatc aaggcttggc ggtaggctgc
    12601 acggctctca ggatttccaa gagtgttgag gtgcaatttg acaccttgaa taccgatttc
    12661 cttcaaaaaa tgggctgcca tagcgattgt ttccacatcg gtagctggat tgctagagcc
    12721 aaaacactca acaccaatct ggtggaattg gcgcaagcgc cctgcctgtg gacgctcata
    12781 acggaacata ggtcccatat agtagaactt gcttggcttt tgcacttctg gggcgaagag
    12841 tttattttcc acataggaac ggacaacggg cgcagttcct tctggacgga gggtaatatg
    12901 acggtcaccc ttgtcataga aatcgtacat ttccttggtt acgatatccg ttgtatctcc
    12961 gacagagcga ctgataacct cgtaatgctc aaaaatgggc gtgcgcactt ctgcgtagtt
    13021 gtagcgcttg aaaatttcac gggcaaagcc ctcaacgtac tgccatttgg cagattcagc
    13081 aggtaaaata tcctgcgttc cttttggttt ttgtaatttc atagggaatc ctctttaaac
    13141 ttaatagtct tattttacca taaatagagg gattaaaaca gtgagaaaaa aattaggatt
    13201 caaatatcat ttttgagatt aagacctgtc aaaataatag ctagcaagga aataccaaca
    13261 aatagcatcc aagtcaactg tatattccat acggctacaa gtgaaaaaca ggctgttccc
    13321 acaggtatgg ataaggtaaa caagagacct aaaaagttac tggtacgagc tagaacctct
    13381 ggagctagat ttttcatgag catggtacta atcttcggtg aaactttacc agacacatac
    13441 aaagttaaaa agagaaatag caaaccaaga acgacttgat tgaataaatt agccaaacca
    13501 actagactaa gtcctacggt cgcccacatc atcaatctag gcaaggactg cttcccaaaa
    13561 taatcattgc ccgtaaggct actgatgatg attgctacta aaacacagaa ttgattgata
    13621 aatagtgcct ctgtgtaaga aaaattcaag agaggatggc tcaaaaagaa gatattataa
    13681 attccaccta aagcgccacc caaggaatta ataaacaaga cagcaaagag cataaaacca
    13741 aagtttttct gtccactttt aagaaaaacg agacgtaaat ttcggtaaat tgttaggaac
    13801 tggtctttga tagaaagctt ctcatttttt aagttttcac catcagcaga tgacattgac
    13861 aggctcaatt tgctttttcc taaaaagagg atagtggctg atactaggaa aaagcaggca
    13921 ttgattccca caacgagaga aaaattgttg accgatagag ctaagaacca gattccgaaa
    13981 gcttgaccac caatagctga aatataggtg atgaactgtg aaaaagaata agcctccatc
    14041 agatcatctt cagctacttt ttccttaata agaggcatac gcaggccacc tgcaaaatca
    14101 ctgatgacat cactaatgac attgatcaaa cacaggctag aaaaggcaaa gagactagct
    14161 tgctgaacaa ctagggctgc tagaaaaaat agaaccgcct gaaacaaacc gctatagacc
    14221 atccatttga ccttgtccct cgtgtaatct gcccgaatcc ctgcaaaaac tgtaaagaag
    14281 gtcggaagaa tcatgacaat attcgccata gcaacagcaa aagatgcttg tgacaaggtc
    14341 gatgcataga cgataaagac caggttgaaa atcgaagcaa caaaagcatt gaagaagcgt
    14401 gacaaagtca aaattctata agctggattt ctaaacaata gtttcataga taatctccct
    14461 ccttgttcga acctctatat gaagtatgac tatagtatag cacttagaaa tttgagaggg
    14521 agaactcttg aatatatgca acaaataaaa tgagcaaaaa gaaacgatag aacttttgta
    14581 atgacctgag agagggcaat agttctatcg cttcaaattt tttcttggtt tgcagatatt
    14641 caacattcgg tcgtatttct atagtattcg gcagatttat tacagccaag catctcaaaa
    14701 atacggacag catcctccat ctttttctgg ccttccttga ctctaccttg cttgctatca
    14761 aggagacctt ctgcccacag ataaacaatt cggaaatagg tctcattttc cttgtagaaa
    14821 tgctcttcga taacacgttt aaaataatag gcattggtaa attcttcaca ctcaatacta
    14881 gctaaaaagc cattcaatag tatagtatga aaaaagtttc gattaccaga catttccatt
    14941 agaaaatcag atttacgtac catttctcgt acatatctag taaaaagaga aacagataaa
    15001 aaggcagaac tgactgaaaa taaattgagt tcatagattc cccagatctc ggtagaaaac
    15061 aaataatcat gaaggacttt tccttcctct gctgttaact ctaccctttc atccatgctc
    15121 ttcatataag acttgatcat aatggcattt agaatatgtt tctgtttgtt gtgagaatgg
    15181 gcatgctttt gatactccct gcgatataag tcctcaagag gtgctatatt ctttggttcc
    15241 aagacatctg taatttcttt tctcaactca gaatctgtat catactggaa acctctcgcc
    15301 agaaagagga tctcctccac actggcagat atattttcca gagcaaatag aaacttttcc
    15361 accgaaagct cactctgacc tgtttcaaaa cgggacaaca tagacggcga aaattgtcct
    15421 ccggttgctt gtctcagtga gatatttctt gattctcgta attgtctaaa gacttttcca
    15481 atctgctcca tagacttccc cttgattccg tattttcttc attttatcat atttttcaga
    15541 aaattcatca aaaacttgcc aaattgtcag aattatgaga aaatagagga tatttatcac
    15601 gtggagggac tgctatgaga gacgatatca aaatcaatga ccgtgctttg gccttgcaag
    15661 accaaattat cgaaaaacta gagaaagttt ttgatacaga tgtggaattg gatgtttaca
    15721 atctaggtct gatttatgaa atcaatctgg atgaaacggg gctctgcaag attgtcatga
    15781 ccttcaccga tactgcctgt gattgcgccg aaagcctgcc tattgaaatc gtggcaggtc
    15841 tgaaacaaat cgagggtatc aaagatatca aggttgaagt tacctggtcg cctgcctgga
    15901 aaatcacacg aatcagtcgc tatggccgta ttgcccttgg actaccacct cgttaagcag
    15961 gccaatcact tttaaagatg aaaaaagcaa gccgaaaatg gcttgctttt tgatactcaa
    16021 tgaaaatcaa agggcaaact agaaaactag ccgcaggttg ctcaaaacac tgttttgaag
    16081 ttatggatag aactgacgaa gtcagctcaa aacactgttt tgaggttgta gatagaactg
    16141 acgaagtcag taaccatacc tacggcaagg cgacgttgac gtgatttgaa gagattttcg
    16201 agtatgagtt tattttttac ctgacttgtc catattccag aagtctgtca cggctccgcg
    16261 tgaagcagat gatacgatgt gggcatattt accgaggaca ccacggctgt aaagtggtgg
    16321 caaggttgtt tctgccttgc gtttttcaag ttcttcttcg gatacggcca tggaaatttc
    16381 tttggtatct tggtcaaccg taacgatatc gccggtacgg agataggcaa ttggtccacc
    16441 atcctgagct tcaggagcga tatgtccaac aaccagacca taagtaccac cagagaaacg
    16501 tccgtccgtc aagagggcca ccttatctcc ctgaccttta ccaacaatca ttgatgaaag
    16561 tgacagcatc tcaggcatac caggaccacc tttaggtcca acaaaacgaa caacgactac
    16621 atcgccatca acgatttcat ctgtcagaac ggcctgaatc gcatcttctt ctgagtcaaa
    16681 gaccttagct ggcccaacgt gacgacgcac tttaacacct gataccttgg caactgcacc
    16741 gtcaggagca aggttcccgt tcaagatgat aagcggacca tccgcacgtt ttggattttc
    16801 aagtggcatg ataacttttt ggcctggagt caagtctgca aagtcagcca agttttcagc
    16861 tacagtctta ccagtacatg tgatgcgatc tccgtgaagg aaaccatttg ccaacaagta
    16921 cttcataacc gcagggacac caccgacttc gtagaggtct tggaagacat actgaccaga
    16981 tggtttcaag tcggccaagt gaggcacacg ttcttgaatc gtattgaagt cctcaagtga
    17041 caagtcaaca tttgcggcat gggcaatggc gagcaagtga agagtggcgt ttgtagaacc
    17101 accgagagcc atggttacag tgatagcatc ttcaaaggct tcacgagtca agatatctga
    17161 tggtttgaga ccaagttcca acatcttaac aacagcacgt cctgctgctt cgatatcttc
    17221 tttcttatca gctgattcag ctgggtgaga ggatgaccct ggcaaactca tccctagaac
    17281 ttcgatagca gttgccatgg tattagcagt atacatacca ccacaaccac cagggccagg
    17341 gcaggcatta cattcaagac gtttcacgtc ctcagctgtc atgtcaccgt ggttccattt
    17401 tccgatacct tcaaagacag aaaccaagtc gatatcttta ccatcaagat ttcccggtgc
    17461 aatagttcca ccataggcga aaatagctgg gatatccata ttagcaatag caatcataga
    17521 tccaggcatg ttcttgtcac agccaccgat agcgacgaag gcatccacgt tgtgaccact
    17581 catagccgcc tcgatggagt ccgcgatgat gtcacgagat gttagagaga aacgcatacc
    17641 aggcgttccc atagcgatcc cgtccgctac ggtaatggtt ccaaactgta caggccaagc
    17701 gcctgcagat ttgacacctt ctttagccag tttcccgaaa tcatgcaagt gaatgttaca
    17761 tggtgtattt tccgcccaag tcgaaatcac tcccacaatc gatgtttcaa agtccttatc
    17821 tgtcatacca gtcgcacgaa gcatagcacg gttaggtgat ttaaccatgc tgtcatagat
    17881 gctactgcgg tgacgtttat ctaattcagt catcttatcc ctcccatttc agtttttact
    17941 attatagcac aatttaaagg caatgaacag aataaaattc ttgaattttc agaaaattct
    18001 atacgcatgt gaaatattta aaattaaaaa caacaaagcg gattagtgca ctttctgatg
    18061 accagaatat gctttttaat ccgctttctt taaataacgt aatgtaattt ttacagaaat
    18121 tctttcagat aagtgtattt aacatctatc ttgcattata aatttctaga accttctctt
    18181 ttatattcga ttcactcaaa ccatactcat taagaagata atccattttc cctacttgac
    18241 caaatctttc ttgaacaccc atccgatgaa tttttgttat tccatcatca gagaataatt
    18301 cacataaagc actgccaatt ccacctatct gattgtggtt ttctacagta aatacagttt
    18361 ttccacttaa cattgttttt atctgttctg gtatcggttt gattctaaat aaatctatca
    18421 cacctactga ataacctaat ttagacagtt catctgcaac tcgaatactt ggagcaacca
    18481 ttatgccaga agcaactatt acaacatctt caccgtgtct taactcaatg tagcctttag
    18541 aaaaatcttc tccaccttga tatacagcct ctggagcttt tctaattgtt cgaatatatt
    18601 ttagtccttt taagtctaat gtctggttca atatttcacg aaattggata tcatcagttg
    18661 cttcaaaaat gattgattta ggaattaaac gtaacaagcc gatttcttca aatggcatat
    18721 gtgttccacc attcatctct gccgttactc ctgcatctga tccaatcaca gtggcatcca
    18781 attgtgcgta tccaagagaa ataaataatt gatcaaatac tcttcgtgaa gcaaaaggac
    18841 caaatgtatg aagataaggt ctaaacccct gaatagacaa gcctgctgca aggccgacca
    18901 tttctgcttc cataatccca acattcacat aacggtctcc gaagtccttt tcaagattat
    18961 gagtagccat cgaacttgac aaatcggctt ctaagactac tatatcagaa taactttgat
    19021 tagcctctaa aaggaagtct ctatatacat gccgtaattc tttcgtactt ctcatcattc
    19081 tgtttcctcc aattcctgac ttaatctttc tacaactgaa gttaacattt gtttctcctc
    19141 tacagtaggg cgaagatgat gattggattt catttcttcc agctcttgaa ccccttgacc
    19201 tttaatagta tctaatacaa tgcacttagg tgatgaatta tttgactgtt ttaattggac
    19261 aatcccttca taaatttctc taatatctga acccttgacc ctaatggatt caaatccaaa
    19321 tgctgaaaat ttttctacga aatcacctga attacaaata tcctttgtaa aaccatctaa
    19381 ttgttttttg ttatcatcaa caaatacaat taagttggat aactgttgat gagaagcaaa
    19441 ctgtatagcc tcccaacatt gtccctcatt taactcacca tctccaacaa tagcgtaagt
    19501 ataaaaggga ctctttctta ttctctgacc atatgcaagt ccagttgcaa cactaattcc
    19561 ttgtcctaaa gagcccgttg tcatatctat gcctggcgtt agatttctat caggatgaga
    19621 cggtaatttg gttccatttg tatttaaaga atataagaat tctttgtcaa agaaaccatt
    19681 caaatagagt gtactgtata gagctggtcc tccatgacct tttgataata tgaaataatc
    19741 tctatctcgt gctgcaaata tttctggagt cattggcatt atttcaccat aaagcactgc
    19801 taaaacttct acgatagaca aacttcctcc gtaatgtccg aatccaagat gattcaatgt
    19861 tctaagagta tttaatcgga tgttagtcgc aaattttctt aacccatctt ctctattttt
    19921 acttaaaatc atcccttatt cctcctttgc agatggcttt ttaataaagg atactccaaa
    19981 cataactgct aaaataagaa caagaccaat cacaatgcct gcttgtgagc caaattgatt
    20041 taacattcct aaaataattc ctgatagacc aaaatctgca tctgagaaag ttgatccttg
    20101 gaaaccaagt cctcccaaaa ctggcattaa aaagactgga agaaaactga ttaaaatacc
    20161 ttgtaaaaat gctccaatag tggctccacg aacaccacca gatgcattcc caatgacacc
    20221 tgcagtcgct ccacagaaga aatgaggcac aacgcctggt aagataacaa ccgttcctga
    20281 agcaatcata attgccatac ttactaaacc accaacaaaa ctagagataa atccaattag
    20341 aactgcattg ggtgcataag tataaacaat cggacaatcc aaagcaggtt ttgaattagg
    20401 tacaagacgc tctgaaatac ctttaaaggc tggaacaatt tcgcccaaaa taaggcgaac
    20461 acctgctaaa ataacaaata cccctgctgc aaattgacct gctaattgta aagcataaac
    20521 tagaccactt gtaccactac tgatttcttt ttctatatat tctgaccctg caaagatagc
    20581 tacaataatg taaataactg ccatagataa agtgatacta acagtactat cacgtaaaaa
    20641 agctaaactc tttggaaatt taatgtcctc tgttgatttt gatttgtcac cgataaggct
    20701 accagtaaaa ccactcaacc aatatcccaa agaactgaaa tgacctaaag ccaccttgtc
    20761 atttccagtt aattgaacca tatatttttg cacaaatgct ggggaaatac tcataataat
    20821 accgagtgct aatcctccta gtaagataag aggcaagcta gtaaagccag caactgataa
    20881 aatgaccgca atcatacacg ccatatatag agtgtggtgc cctgttaaaa agatatattt
    20941 aaatcgagta aaacgagcga ttaagatatt gaacaccatg cctgcaaaca taatcattgc
    21001 agtagctgag ccatatgttg ttaaagctac agctacaatt gcttcattat tcggcacaac
    21061 gccagataaa tgaaaagcat gctcaaacat ggtaccaaat ggatttaaag aattttgtac
    21121 aattcctgca ccaccagata caactaagaa accaacaaag gtcttaattc cacctttaat
    21181 aatatcaggt aatttcttct tctgaagaac taatcctaaa attgcaatta aagctactaa
    21241 aatagctggt gtactaacaa tatccaatat gaacttcatc atgacgctag cctcctatat
    21301 aagtcctttt tcttcacaaa ttttaataat taattctcgt agttcgtcca tatcaataat
    21361 actatttaag atacgaacat ctccaagatg actagctgaa tcagctagat cacgaccaac
    21421 aatccaaata tcagctgcat ttggatctgc tccacctaaa tcataatgtt caacttctac
    21481 atccgaaaca ttcaaatcac tcaatacaga ttcaatattc atctgtacca taaaacttga
    21541 acctaatcct gaaccacaag ctgtaccaat ttttaacatt atctaatcct cctgtttaat
    21601 tatcatttta atatcatcat agttttttga tgatattaaa gtttgaacat gatttttatc
    21661 tcttaaaatt gttgttaaat gtgacaaagc ctttaaatga ctctcattat caatggctgc
    21721 aatacaaatc aacaatctta cctcttgttc tggattatcc aataaataaa tcggttcttc
    21781 caaaactaac attgacattc ctatttcatt cacaccttca tctggccgag cgtgaggaat
    21841 tgctactccc ttccctaaat taataaaagg tccaaactct tctacttttt gaatcattgc
    21901 ctcagggtag ttctcagtta tcttatcttg atccaaaagc ggtttagctg ctaaacgaat
    21961 cgcctccttc catcctaatt tttgcgaact aacctgatag gtttctttgg taataagttg
    22021 ttctagcact ggtacaattt cctttctatc atttttttga taaagataat tctttaacgc
    22081 caatcttaat tccaattctt gtgtaataat tccatatctt ttgacaatat tcaggatttg
    22141 ttcaatctca aaatctccat actctaaatt cggaaaatct tttaacacta gttctactag
    22201 ttgtattgct tgctcttcag tcatcataac cgaaactaga taatttggct tttctgtctc
    22261 cacttttatg gtagaaaaaa ccatatcata gtcactacta gctttcacct gtaaatcatc
    22321 aatctttgag gttcctataa actcaatttg aggaaataat gctaatagat tctcttttaa
    22381 catcaatgaa gaactaacac cattaggaca aatgattgct gctttatagc atttttgagg
    22441 caaagtatct gctttcttta aataacctcc gaaatggata acaaaatatg ctgtttcact
    22501 atcaggtatg ggattgtcaa tagcgtccat caagggcatc aaagaatctt tgactaattc
    22561 aaataaatca ggataatgtt ctttaacatg caatacatat tcatttgaac taggtaggcc
    22621 gaactttaat ctatagtaag ccggtataag gtggcggcga agattttctc tcaatccttc
    22681 cctttgttta aaatgtaaca aagaaatatc ttccattcta cttataatag cctctgttaa
    22741 ttgattaaag taaaccggag caacatctac ttcaccttca aagcaacttg ataataaaac
    22801 ggtgatatag cgataatcat cctcagaaaa caccgtatct ataattccca aatcaaccac
    22861 tgtatccaat aaaatagtgg ttatatcttg aataacagga gatactaatg tctctgaaag
    22921 acatactctt tcaacatccc tttgatacct acacagaatg aatactaaac cgaaaaggta
    22981 aacttttaat tgattaacaa taggtactag ctgtagcttc tcataataat ctttaactac
    23041 ctgatcaatc aaatcataag ttaatgaata cccccaactg gataaaacat aatccaaacc
    23101 ccaaatccct atggaggatt ccagcaactc actaaccatt tgaaaagcta aacggtgctt
    23161 attccactct gaaccgtgta aagtataacc ttttgctcta ctgtacccta gctccaaatc
    23221 attatctaac ataatctttc ttaatgattg aatatcagat aaggttgtat tcttacttac
    23281 tttcaaaaag tcttggtaat gattattcga tataaaatct aatcggcaaa aagtgtaaag
    23341 atagattaaa gctaagcgag tcgactttgg taaaaccaat tcatccgact taataatatc
    23401 tgtcaaagac tgcttcgtac gatttgataa actatatcga ccttgttttt tatccagcac
    23461 tatcccttta ttagctagat aaggcactaa ataatctatt ccttctttga cttcctttat
    23521 aggtaagctc accttaacag ataattcata taatgatagc tcacaatgat ccatcaaagt
    23581 catcaaaata actagtgctc tataatcaaa catttctctt cctttctaac tacaattata
    23641 gtgtaaaaat tataagaata aaagagtatt tgagcacaat ataatctcac cttttaaaag
    23701 aaattattgg gctgaaactt tttagtgatt attttcaact ttctattgac ttttaaaaac
    23761 aaagtgatat catttagata tcaaataatg ggaggtcatt cttatgactg aaaaactaat
    23821 caattcaaaa ccaaatggtg tattcgcatt gattctcatt gaattgacgc tcgtacttgg
    23881 tgtctttata tttataatgg gtgttggttc ggaaaacatt tttggaatta ttatcggacc
    23941 tttactaatc gtaattgcag cgctaactca tgctggttta aaagttgtca aacctcagga
    24001 ggctctggtt ctgacactct ttggtaacta tacaggtacc atcaaagaac ctggctttta
    24061 ctttgtcaat cccttcagcg tagcagtcaa ccctgcaaac cacactcgac ttggacaaag
    24121 tggtgatgtt agcacaaaat ctcctttttt aggagctaaa tcatcaaatg gcaatgatgt
    24181 aaatcttgaa attggcaaga aacacatttc cctcaaagtc atgaccttga gcaattctcg
    24241 tcaaaaaatc aatgattgct taggaaaccc tgtagaaatc ggtatcgcgg taacttggag
    24301 agttgtcgat accgctaagg cagtcttcaa cgttgataac tacaaagaat atctttcatt
    24361 gcaatgtgat agcgccctcc gtaatattgt ccgcatctat ccttacgatg tgtctcctaa
    24421 tgtggatact acgggtgacg ggcaagcaga tgaaggtagt ctccgtggct ctagcgaaat
    24481 tgttgctaac cgtattcgtg aagaaatcca aagtcgtgtt gaggatgctg gcttggaaat
    24541 ccttgaagca cgtatcactt acctagctta tgctccagaa attgctgccg ttatgcttca
    24601 acgccaacaa gcatctgcca ttattgatgc acggaagatg attgtagatg gtgctgtagg
    24661 aatggttgaa atggcactag aacggctcaa tgaaggggaa ttggtagaac ttgacgaaga
    24721 acgaaaagct gccatggttt caaatctcct tgtcgttctt tgtggcaatc atgatgcaca
    24781 accaattgtc aacacaggaa gtctttacta aagatggctg aagctaaaaa aaagcagatc
    24841 ccccttcgac tctcaacaaa gttatacgct gcactcgcat catgggcaga agacgacttt
    24901 cgttcagtca atgggcaaat cgaatatctc ctcacagagt gtgtcaagca acggaaaaaa
    24961 gacggaaaat acgtatcaga aaccattgac gagccatttg agattgatat ttaacatctt
    25021 ctcctgtctg ccgtgtcgga taggagaatt ttttcatctt tttttgtcaa gtaactttac
    25081 tttacaaaaa aaatgtgtta tcctagtatg gttgatgaaa atcagtagat tgaatcgaat
    25141 ataaatacct aaaggagaaa tcaaaatggc agtacctgca cgtcgcactt caaaagcgaa
    25201 gaaaaacaaa cgtcgtacac actacaaagt aacagctcca tctgtaaact ttgacgaaac
    25261 tactggagat tactcacgtt ctcaccgtgt atcacttaaa ggatactaca aaggacgtaa
    25321 aatcgctaaa gctgcatcag ctgaataata gaagggagat accatgcgcg taaatattac
    25381 acttgaacac aaagaatctg gtgaacgctt gtaccttact tctaaaaaca aacgtaacac
    25441 tccagaccgc cttcaattga agaaatactc accaaaactt cgcaaacacg ttgtgtttac
    25501 agaagtgaag taagcattca atacgaatca atacagaaga aaaacgcttg atcagcgttt
    25561 tttctttttg ttaatttcac tatattgcaa gggaaatcat tcgaatgact acctttttca
    25621 ctaccttttt tgaaataaga atacggattc ggacgattaa aagtaagaga aactcaaatg
    25681 taagttgggg ttcctttttt gtatattttt caaataacgc ctctacacta tttgtagcaa
    25741 attcaccaac tacagttgta tcttagttaa aataagttag aatatgtaag tgagtaccag
    25801 atataccaag acatcgtcac catctaaggt atattcaaaa tacaaaagtt gaccaactag
    25861 atttctgaat atccttatat atccattctt aaaattggtt taaatagcgt agtcttttaa
    25921 actagttttg agaatccaaa aaatcttcct acatatgtaa gaagattttt tagttcagaa
    25981 tgattagatt tagctaatgg atacctatcc tacccactca ccgttagcat tgactcgata
    26041 gccatcgggt gtagtcgtgt ttactaataa atctcctgaa ttataggtat agtaccattt
    26101 accagaaact gtaaaccaac cagtagccat tgaacctgat tcatttaagt aataccataa
    26161 gcctttgtct ttaacccaac cagta
//
LOCUS       DAVMAF010000023        25425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMAF010000023 DAVMAF010000000
VERSION     DAVMAF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.023"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(159..317)
                     /locus_tag="VYO97_000958"
     CDS             complement(159..317)
                     /locus_tag="VYO97_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753772.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(327..1637)
                     /gene="obgE"
                     /locus_tag="VYO97_000959"
     CDS             complement(327..1637)
                     /gene="obgE"
                     /locus_tag="VYO97_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1753773.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENIEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(1701..1835)
                     /locus_tag="VYO97_000960"
     CDS             complement(1701..1835)
                     /locus_tag="VYO97_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1753774.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(1894..2070)
                     /locus_tag="VYO97_000961"
     CDS             complement(1894..2070)
                     /locus_tag="VYO97_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1753775.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(2101..3426)
                     /locus_tag="VYO97_000962"
     CDS             complement(2101..3426)
                     /locus_tag="VYO97_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1753776.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(3442..4485)
                     /gene="cpoA"
                     /locus_tag="VYO97_000963"
     CDS             complement(3442..4485)
                     /gene="cpoA"
                     /locus_tag="VYO97_000963"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1753777.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(4600..4929)
                     /locus_tag="VYO97_000964"
     CDS             complement(4600..4929)
                     /locus_tag="VYO97_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1753778.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(4943..6052)
                     /gene="rpoD"
                     /locus_tag="VYO97_000965"
     CDS             complement(4943..6052)
                     /gene="rpoD"
                     /locus_tag="VYO97_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1753779.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(6055..7815)
                     /gene="dnaG"
                     /locus_tag="VYO97_000966"
     CDS             complement(6055..7815)
                     /gene="dnaG"
                     /locus_tag="VYO97_000966"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1753780.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLTQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(7905..8663)
                     /locus_tag="VYO97_000967"
     CDS             complement(7905..8663)
                     /locus_tag="VYO97_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753781.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMGLTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(8660..9526)
                     /locus_tag="VYO97_000968"
     CDS             complement(8660..9526)
                     /locus_tag="VYO97_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1753782.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPIF
                     KQTILKGAKLSK"
     gene            complement(9595..10599)
                     /locus_tag="VYO97_000969"
     CDS             complement(9595..10599)
                     /locus_tag="VYO97_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1753783.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(10712..10929)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11012..13708)
                     /gene="ppc"
                     /locus_tag="VYO97_000970"
     CDS             complement(11012..13708)
                     /gene="ppc"
                     /locus_tag="VYO97_000970"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1753784.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(13728..14957)
                     /locus_tag="VYO97_000971"
     CDS             complement(13728..14957)
                     /locus_tag="VYO97_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1753785.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(15113..15415)
                     /locus_tag="VYO97_000972"
                     /pseudo
     CDS             complement(15113..15415)
                     /locus_tag="VYO97_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(15520..15809)
                     /locus_tag="VYO97_000973"
                     /pseudo
     CDS             complement(15520..15809)
                     /locus_tag="VYO97_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            complement(16058..16645)
                     /locus_tag="VYO97_000974"
     CDS             complement(16058..16645)
                     /locus_tag="VYO97_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1753786.1"
                     /translation="MGGNPPMKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            complement(16724..17151)
                     /locus_tag="VYO97_000975"
                     /pseudo
     CDS             complement(16724..17151)
                     /locus_tag="VYO97_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(17207..17509)
                     /locus_tag="VYO97_000976"
     CDS             complement(17207..17509)
                     /locus_tag="VYO97_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753787.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            complement(17784..19523)
                     /locus_tag="VYO97_000977"
     CDS             complement(17784..19523)
                     /locus_tag="VYO97_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1753788.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKIPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            complement(19686..20570)
                     /locus_tag="VYO97_000978"
     CDS             complement(19686..20570)
                     /locus_tag="VYO97_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1753789.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(20682..21536)
                     /locus_tag="VYO97_000979"
     CDS             complement(20682..21536)
                     /locus_tag="VYO97_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1753790.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(21594..22352)
                     /locus_tag="VYO97_000980"
     CDS             complement(21594..22352)
                     /locus_tag="VYO97_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1753791.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(22414..22602)
                     /locus_tag="VYO97_000981"
     CDS             complement(22414..22602)
                     /locus_tag="VYO97_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1753792.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(22923..23489)
                     /locus_tag="VYO97_000982"
     CDS             complement(22923..23489)
                     /locus_tag="VYO97_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753793.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(23757..>25425)
                     /locus_tag="VYO97_000983"
     CDS             complement(23757..>25425)
                     /locus_tag="VYO97_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1753794.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYG
                     VNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEDRIQKAREGKWNGGFAP
                     YGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPL
                     FDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDE
                     VWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKY
                     KDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMK
                     VDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDK
                     IDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEI
                     QVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
BASE COUNT         7522 a         5574 c         4095 g         8234 t
ORIGIN      
        1 agaaatcgat tttactgtcc tgatcgattt gtcctgttct catttcattt tactatattt
       61 tagaagatat gctgtgctat tccagattca gttcgctata catgcccgtc gtaccacaaa
      121 atcccgtgtc tgatgaggca cgggattttt tagcttcttt actgagatgg attttccttt
      181 ttagttcgtg ccaatctcaa ggcacgattt ggattgagac gattaaatag ttcttccaat
      241 ttcttttcat tccaaacgtc tgcggcggaa acaaaattac catccgcatc tcggaaagat
      301 atcggtttat ctcccatacc agtctcctag tctacaaatt caaactcaaa tttaccaatg
      361 cggaccaaat ccccatcttt agctccacgc gcacgaaggg cttcatcaac ccccatacca
      421 cgaagctgac gggcaaattt catgacagat tcatcacgat caaagttggt catattaaag
      481 agtttcatga gtttttcacc agaaagtacc catgtcgcat cgtcatcacg actaatttca
      541 aaggcttttt cttcttcgtc aaatccatag taagcttctt cttccatatc ggactcgtcg
      601 tagagcaaaa attctggtgt cttgtctaac aattcagctg tagcatctaa aagtgttgcc
      661 agaccttgct tggtcaatcc agaaattggg aagatagctg gtaactcttc aaattcatca
      721 taattttcag ccaatttttt cttaaagtct tcaatatttt cctgactctc aggcatgtcc
      781 atcttattag ctacaataat ctgtggacgc tccatgaggc gaagattgta agactccagc
      841 tctttattga tagctaggta atcctcatat ggatcacggc cttcgctagc tgacatatca
      901 atgatgtgaa ggataacacg tgtacgctcg atgtgacgga ggaactgagt tcccaaacca
      961 acaccttgac tagccccttc aatcaaacct ggcaagtcgg ctactgcaaa ggattcacct
     1021 gattgggtgc gaaccatacc taaatttggt acaatagtgg taaagtggta ggcaccaatt
     1081 ttaggcttag ctgaggtaat aacacttaaa agtgttgact tccctacaga tgggaatcct
     1141 actaaaccga catctgccaa gatttttagt tccaattgta actcacgttc ctgacctggt
     1201 tctccatttt cagagatttc cggtgcagga ttttttggtg tcgcgaaacg aatatttcca
     1261 cgtccaccac gaccaccgtg ggcaacgata aattcttgcc catgttcaat caaatctgtt
     1321 aaaaccttgc cagtctccgc atcacgaaca gtcgtacctt gtggtactcg aactctaagg
     1381 tcctcagcac cacgaccatg catccctttg gtcatccctt tttcaccaga atcagccttg
     1441 aaatgacgat tgtagcggaa atccatcaag gtacgtagtc cttcgtctac aacgaagacc
     1501 acattgcctc cacgaccacc atcaccaccc caagggcctc cattagggac atatttttca
     1561 cgacgaaagg caaccatacc atcgccacca ttaccagcct tgaccttaat cttagctgta
     1621 tctaaaaaca tactcattat ttcttctcac tttaaaaaag ggctgggaaa tcccagtcac
     1681 taaattttct tgaatctatt ttatagatta ctgagggcac caattgcagt tgcaaaaatt
     1741 cccaataaac ttgctactag catgataatc acgataaaca aggttatttt ctcaaacata
     1801 gtttttttac gatttccatt atctccaaat gccatttttc tctccttcgt tacattctat
     1861 tttctattat cttagcatga attgagctag ttttcaatag tcagttgcta ctggtgcgat
     1921 aatccataaa ataatgtaag ctacaattcc agctccgtaa cagcaagtaa gaacacccca
     1981 aatgacttga acgatagtcg gatccatatc cagataatgg gccaccccag cacaaacacc
     2041 cgcaatcttt ttatcacgac cgcttctcat taattgtttt tttcatagct gttcctcttt
     2101 ctattcttca tggtctttcc aatagtctct catactgatc aactgtgttt ttgaagcctt
     2161 caacatgcgt ctagatcctt ttacaggtac agcaaccacc tgttgctgaa gatacaaaac
     2221 tgtcttaaag atacgctgac tgaccgtatc atctttagcc aaatctttct ggaagttatt
     2281 tgaaataatg gcatccagat aaaactcatg cactcgtttc ccaaagttct cagctgaaat
     2341 ctcatacaat ttctctgata aggtatgctc gttcatgtct ggtgttgcaa tcagggcttc
     2401 caaaatagca ccagccaaat catgttctcc atagtacaag gttccaaaca ttttatcact
     2461 gatgaggttg ttcaaataag gatttccgtg agcaatgaca ggtgttccac tggctaagct
     2521 ttccaagtag gtcaaacctt gcgtttcgct tgtcgatgct gaaatgaaga aatccgccgc
     2581 tttatagtaa agagccgtct cactaggagc aatcatccct gtaaagatga ctgagtcttg
     2641 aatctctagg ttctgggctt gctctttgag gtcattcaga taagggccat ccccagctac
     2701 taccagttta accttgtctt cctctttcag aacatcagca aaggctgcta aaactgcttg
     2761 aatatttttt tcataggaga ttctcgaaag actaagcaac gtcttttcac catcttgaat
     2821 ccctagttta ctacgcagtt ctttcaaatt ttcctgcttg atttccggac gctcaaactt
     2881 ggctaattca atcccagtag gaatgacccg tttttcaacc ttgaccttat aatcagatag
     2941 caagtcacgg acaatctcac tagggcaaat aaccccatcc acatcatgca ggaaacctct
     3001 aaccagatac ttgaccatac tcggccggat caacatcccc ttagcaatat aatggacata
     3061 gtcttcatac tgggtgtgat aggtatggat gactggaatt ttcaattcac gcgcaatcca
     3121 aatccccaac aggccaagag aaaattctgt ctgagtatgg ataatatcta gctgatactg
     3181 tttagcaatt tcaagtgcct tgctaaaacc tcggtaggca aagcgacgat ccttaaaagc
     3241 aaagaaagga acacttggaa tgcggataat ttgccaatct tcgtagcgat tgacatcctt
     3301 atctgtcgtc gtaaagataa aaacagcatg tccctgcttt tcaagttctg ttttcaaggt
     3361 tcgaatactg gtcgcaacac cagaaacctg aggaaaatag gtatctgtaa ataaaccaat
     3421 tcgcatagat tacctcactt tttactttct ccctaaagcg gcttgtttct cataaaaatc
     3481 caaccagatt tgtaacagat gctcttcaga atactctctg gaaatattct tagccttttc
     3541 tttgagatct tttaaggcag caggatttgc ttgatattcc aaaatagcct ctttcatctc
     3601 ttctctaccc gctgtcgccc gataatttcc ctccaaaatc accttataga gatctaaatc
     3661 acgcaacata ataggagcct cacaactcgc agcttctaaa atagtcatag gaaagagctc
     3721 attgtaacta ggcaacaaga aaagatccgc tagagcatac aattcgcgca tccgctctgg
     3781 cgatacaatg cctggaaaaa tcaaattttt agggggattt tccataattt tcttatagtg
     3841 ttcataacca tctgtcatac caccaaaaga gaagccacca gcccagataa aggtaatctg
     3901 aggcaattcc tcagccagac ggataaagtc atcaatccct ttacgtttct gaacttgccc
     3961 agcacctact acgataaact gattgtcact aagaccaaga tctgtgcgca gtctgactac
     4021 ctcttcttgt ggtagaggat gccatttttc cttgttgaca aagttaggaa tataggtcac
     4081 tttttcacgt ggaataccag ctgctaccaa atcctcaata aacataggat tgaccacaac
     4141 caagtgctcc atccggttgt aaaaagaaaa tacatagcgt ttcacaattc cctttaagaa
     4201 aaatggaatt ttcaaacttc cctcaagtgt agctggcaag aaatgcacat agccaatctt
     4261 tctccctgag cgtttctttt ggaaggttga taaataatag ggaaaatcaa tcgtatgaaa
     4321 gtgagtcaca tctgcctcga ttggaagatt ttctgtaaca atcaattggt ccttggcagc
     4381 acggtgaaga agacgaacta attcacggta agcacctgaa actccctgtc ctgctacttt
     4441 ctcacttgaa ctcaacatat tgatgcgtaa tttctttttc tccataacta ctattatatc
     4501 attttctttg aataaaatca gcaaaagaaa ggagagacta gaggaaaaga ggggaaattt
     4561 cctcgctttt ccaacagtct ctcacatgct tttatatgtt tacttaatgc ctagggcaat
     4621 gcgggcatag cgactcattt tttcaactgt ccaggctgga taccagacta atttaacctc
     4681 agtatccgtt acttctggaa cctctatcat agcatcataa atctggtctg tcaaaaggtc
     4741 tgctaaggga caacccatag ttgtcaaagt catgtcaatc tctgtttgcc ctgtgtcacc
     4801 gtcaaaacga atctcataga tcaaaccaag attgacaata tcgattccca actcagggtc
     4861 gatgacttct tccaaggctg ttaaaatccg tgttttgatg ttttcaattt gctcttctgt
     4921 ataagccata ttttcctcac tcttagtctt caataaaatc acgaagcggt ttgctacgac
     4981 ttggttggcg tagttttctc aaagcctttg cttcaatctg acggatacgc tcacgagtta
     5041 cgttaaagac tttccccaca tcttcaagtg tgcgcatttt tccatcatct agtccaaaac
     5101 gtagacgcag aacattttct tcacggtctg taagagtatc taagatttca tccaattgct
     5161 cacgcaagac gatacgagtc gtataatcca ctggattttc aatcacttca tcttcgataa
     5221 agtctccaag gtggctatcg tcctcttcac cgataggagt ttcgagagat actggttctt
     5281 gggcaatctt caagatttca cgaaccttat caggtgtcat atccattcgt tcagcaatct
     5341 gttctggtgt cggatcttgc cccaattctt gaaggagatt ccgctgttca cgaaccaatt
     5401 tattgatagt ttcaaccatg tgaactggga tacggatggt acgagcttgg tccgcaatag
     5461 cacgagtgat agcctgacga atccaccaag ttgcataagt tgaaaacttg aaccctttag
     5521 aatagtcaaa cttgtcaacc gccttcatca agcccatatt tccttcttga atcaagtcaa
     5581 ggaactgcat accacgaccg acatagcgtt tggcaatgga aacaaccaaa cgaagattgg
     5641 cttccgcaag acgttgtttg gcttcgatat caccagcttc aacagccagt gccaactctt
     5701 tctcctcttc attggtcaag agaggaacga cccctatttc tttcaagtac atacggacag
     5761 ggtcattgac cttagcagaa gttgacccaa tcaagtcctc atcgctgagt tctggttctt
     5821 cttcattgct aagaacacgc gcacttggat ttccttcgtt atctgtgata gaaattcctg
     5881 catcctgaat ccgttgcaag agatcttcaa tcccatcagc gtccaaggta aaaggaataa
     5941 ccaaacttgc attgatttca tcatctgttg ctgtcccttt ttgcttatga ttacggataa
     6001 attctgctac ctgtacgtca aatgttgtta cttctttttg ttttgttgcc attattactc
     6061 cattcttctc ttttgggaaa ttaaacgttc caattcttct agggctgtat ctgtatctcc
     6121 tacatggcta gcttcctgca ccttcttttt gattctcata ttgtcctgat tcaagagagc
     6181 cttgtttcga gtcatctcta cttcactaag ttcctgcggc gatatctcag caggcaaatc
     6241 ctgagttaaa acttggtacc aagctctttc aacttcctct gtctgctctg ctaaaacttc
     6301 tggaggaaga tttccatact ggccaagcaa gtcatataag acctgaaatt caggtgtagc
     6361 aaatgcaaag tcttctcgca aacggtaatc gttcaaaaca agaggggatt ccatcatccg
     6421 atagagtaga tgggcttctg ccctcataat agccgataac tgcttggtga taggcatggt
     6481 gattggcgtc ggtctggaaa ttccttccat gcgattctgc ctttgcacct gacgactctc
     6541 attaacaatc tgctcaatct gggtataatc aaaggacgcc agactgtcag ctaaaatatg
     6601 aatatagctg ttttgagcag cgatggactt ttcttgaaca atcaagggag ctattttttc
     6661 aataaactca atctgagcct gcagattttc actgttttca ggtttgtact gatgaatgta
     6721 gaactcaatc ggactaatac gagtttttgt taatagatag gccaagtctt ctggaccatt
     6781 tttttgtaga tactcatcag gatccaagtt atcaggcatg ctaacgattt gcacaggcat
     6841 atcaccaatt tcatccaatg ctttcaatat cgcggcttgc ccagccttat ctccatcgta
     6901 aacaagaacc aatttcttgg ttaacctttt cagatgctca acatgctctc gactcaaggc
     6961 cgttcccatc gacgccacag cattttcgat tccagcccga taggctgcaa taacatccat
     7021 gaatccttcc atcaggtaaa tctcactagc ttttccagaa gatctttttg ccctatccat
     7081 atgatataat tcgtaacttt tgttaaaaat tgcagtcgat cggctgtttt tatacttaga
     7141 agtttgtgaa tccgtttttt gccagatacg acctgagaag gcaatgacct ttccttggtc
     7201 atttgtcagg ggaaacataa tgcgattgtg aaaggtgtct acaaattgat tggcatccga
     7261 gagataaaac aggcctgaat ccagtaaatc ctcttcacga tactgatcag acaaacgttg
     7321 atagagatag tttcgttctg gaggtgctaa accaatccaa aaatgtttaa gcacttcatc
     7381 tgtcaaaccc cgctgataaa ggtaatttct ggcctcttct cccatagtcg ttgtcatgag
     7441 aatagcatgg taaaatttgg ctgcatcttc gtgcatatca taaagagctt ggtgaggcga
     7501 ggctggcttc tgttcactat aaagcggttt ttcaacctca atcccgacac gctgacctaa
     7561 gatttggaca gcctccataa aggtaacccc ttggtactcc tcgatgaatt taaagacatc
     7621 acctgagcga ccacaaccaa aacagtggta aaactgcttg tcctctacaa cgttgaaaga
     7681 aggtgttttt tcaccatgaa aaggacagag ccctagatag ttccgtcctg ccttttgtaa
     7741 agaaatcaca tctcctatga cttccacaat gttggcattg tttttgattt cttcaatgac
     7801 ttgtttgtca accatacaca atacctccat gttatcatag tttactttat atagtatact
     7861 ttatttcaga aaaaaagtaa accatttcac tcattttccc tactttattc aaagagttga
     7921 taataatcag agattttcat ttttgctttt tcttcttggt ttaaatcttg gataatccgt
     7981 ccttctttca tgacaatcaa gcgattgccg tatttgagag catcttccat atgatgagta
     8041 atcataaggg ctgttagctg atctttctta acaaattcat ctgtcaatcc catcaaagca
     8101 acactagtct ttggatccag ggcagcagta tgctcgtcta acaagagtaa ttcaggtcgc
     8161 ttcaaggttg ccatcaagag actcaaagcc tgtctttgtc cacctgataa gaactcaatc
     8221 ggtgtattca agtgtttctc aagaccattt cctacttttt caatggttgc ctgaaattca
     8281 tccttatagc tagtcaagcg tcgtggtaac aatccacgct tttcaccacg aaacttggcg
     8341 attaaaagat tttcagcgac cgtcatacgg ggagctgtcc ccatctttgg atcttggaag
     8401 acacgagaca ggtacttggc acgcttctcg ggtgaaaact tagtgagttc ttcacctaaa
     8461 atacggatag ttccactagt tagtgataag gtccctgcta tagtgttaaa gagggttgat
     8521 tttccagcac catttccgcc caaaatcgtg ataaagtccc gttcaaaaat ttctaaggaa
     8581 acatcattta aaataatctt ttcttcatca aagccatttt taacgatttt ggttgcattt
     8641 tttaattcta caattgctgt catttgctta acttggctcc tttcaagatt gtttgcttaa
     8701 atattggaat catgaggcag actgctaaaa tcaaggcact gtataaacga aggtaacttg
     8761 tattaaagcc aagtgcgata actgcccaca ctaaaaattg ataagcgata gaacctacaa
     8821 cgatagtaac caaacgctct gccaagctca aactcttgaa aataacttct ccaataatca
     8881 aacttgcaag tcccacaacg ataaccccga tccctcgaga cacatcggca tagccttctt
     8941 gctgagcaat gagggcacct gcaagggcaa tcacaccatt tgataagacc aagcccatga
     9001 gctccatgcg tccagtatga atcccgaaac ttctagccat atcaggatta tcccctgtag
     9061 caatataggc ttgtccgagt ttagtgtcca agaaaaagag catgagagca ataacaatgc
     9121 tcacaaagat gagacctgtc aagagttgat tcaaatccga atcaaaaggc aaaacatcct
     9181 gaatttgctt ggttccaagc aggcctaaat ttgcacgtcc cataatcaag agcatgattg
     9241 agtgacaaga agtcatcacc aaaatccctg agagcaaggt tgggatcttc ccttttgtat
     9301 aaagaaggcc tgctgccatt ccagccaaac aacctgctcc tacagcaaca agtgtcgcta
     9361 aaaatgggtt cacgcctttg gttatcaaag tgacagcaac agctccccca agagggaagg
     9421 aaccttctgt cgtcatatct ggaaagttta aaatcctaaa tgtcataaag attcccagac
     9481 ctagaatagc ccagacaaat ccttgagaaa taatggaaac aatcatattt tatttaatcc
     9541 tttctatatt catcttttta aaaaatggga agagtctcct cctccctacc ttatttattc
     9601 gatgacttgt cctgcttctt tgagaacaga ctcaggaata gtaataccta gttcttgtgc
     9661 tattttttta ttgatgactg acttaccagt tgaaaagaca ttgactgggg tatcggctgg
     9721 ttttgcacct ttcaagactt gcacaatcat tttacctgtt gccacaccaa ggtcatgttg
     9781 gtcaattaca actgatgcca aaccacctac ttctaccata gctgtcgcac tgggataaat
     9841 tggtttctta gaactttgat tgctagagac aaccgttgga aatcctgatg caatggtgtt
     9901 atcaattgga acccaaatag catctacctt gctagtcata acagtgacag ttgaggcaat
     9961 ttcatttgtt gaaggaactg caaatgtttc cactgtcaga cctgcttttt cagcataagc
    10021 cttaaattct tcgacctgtg tttttgaatt gtcttcgcta cttgagtaaa gagctccgat
    10081 tgttttcaca ttcggtgtca gagccttgat gagttcaatt tgttgttgag ctggattgtg
    10141 gtcagatacc cctgtaacgt tgccacctgg ttttttcaaa tctttaacca agttagcacc
    10201 aattgggtct gtaatagcgg ccatgataac tggtaggtct tttgttgcac tagccaaccc
    10261 ttgggctgct ggtgttgcga taccaaccac aaggtcattc ccatttgcaa ccaattgttt
    10321 actcattgtc gcaaccttac tttggtcacc ttctgagttc ataaaatcaa ttttaacttg
    10381 atcatcttta tatccttctt ctgcaagtcc atcttggatc cctttataaa tcaaatcaag
    10441 ggatggatgg ctcacaaatt gaagcacacc aatcttagtt attttcttct catccttatt
    10501 attctgagct tctgatttat tcattgaaga ataaatcaag cttcctgcta ctaagactgc
    10561 taatgcagca ataattccaa ttaaacgttt atttttcatt ctgtttctcc tttttatttc
    10621 ctttaacata tttcacttat ctaaacaagc gacgccatcg ttttctttcc atactaacct
    10681 ccatcaaaaa aatcctcaca caaaaaactt gtgtgaggac gtcgatgcgc ggtaccacct
    10741 caattatagg gaatttccct atcgctcagt ctcgcaataa cgagatgcac tgtaaggtgt
    10801 gcttaccgaa tttttataat ttcaaattct aaatagcatt cagcccaatt ttcatcatcg
    10861 ccacttatct gttttcagct accacagact ctctaaaaag tttgtatgat tacttttctg
    10921 aatggttaaa ttatatcatt tttaaactac ttgtcaatag tctattagaa tttttttgat
    10981 attcactttt agcattcact cttgaaaatt atcaacctga attacgcaat cctgtcgcaa
    11041 ttccgttgat ggtgatatga atcaatcgtt cttgatcact ggacaattct ccacgacgtt
    11101 ggcgtttaat caactctaac tgaatatagt tgagaatatt aaagtaaggc atacggtaat
    11161 ccagactagc ttttagatat ggattgtcag ctaagagttc gtcatgtcct tcaatagcca
    11221 agataacgtt cttagtaact tgccattcat ttaaaatagt ctcatagatg gccttaactt
    11281 gctcgtcttc acaaagttta gcatattcaa aagcaatatt catatttgat tttgacaaaa
    11341 ccatatcaac atttgaaaga agcgattgga agaaaggcca attttggtac atatctcgta
    11401 agatagcaat attctctgga tttttattga taaattcctt gaagcttgaa ccaaccccgt
    11461 accatccagg gaacataaca cgactctgtg accatgagaa tacccaaggg atggcacgca
    11521 aaccaccgat ttcagtaata gtcttacgag cggctggacg agaaccaata ttaaaacttg
    11581 aaatagcctt gattggactt gactcgaaga aataatcata gaaatgctca ttaccaaaga
    11641 ccaaatcacg gtagatatcg taactacggt ccactacttg atccataatg gcttcataac
    11701 gatttggggt attggtatcg ctcttcttct gagtaatcat acggttaata gctgccgata
    11761 ctagcatttc aaggttatag taagcggcgt ctttgttacc gtatttattc ccaattactt
    11821 caccctgctc cgtcaagcgg atacgatcct tgatagactt gagcggttga gatgtaatgg
    11881 cttcataggt tggcccacca ccacgaccga cagtaccacc acgaccatgg aagaaggtaa
    11941 ccttaacgcc aaattcatct ccaatagcag tcaattgttg ttgagccttg tagagggtcc
    12001 aacatgatga caagtaaccg ccatctttat tactgtcaga gtagccaagc atgatttctt
    12061 ggtagttatt tcgtgagtca atccattttt tggcaaggct aagagaaaga tattttctca
    12121 ttgtttcttc tgaatgatcc aagtcttcaa ttgtttcaaa aaggggaaca atctgaacac
    12181 gcgccctttc cgtatccacc agtcctactt cttttaacag aatagctaat tctagcatat
    12241 cagaaaggct ggttgcatgt gaaatgatgg tctgacggat gacatcatct cccaacttat
    12301 ctttcaaaac acgagccgtc ttaaaaatag ctaattcttt tgctaataat tctgattttt
    12361 ctgcgtgagt cgcagaaaga attcggggat cttcttctaa ttctttcaag agaaggtcac
    12421 acttttcttc ttcgctcaac tcgctataac gagaatgaat tcctgctgat ttcaagagtt
    12481 ctgccacaca ggcttcatag acgctagagt cttgtcgcat atcaattgat gctaggtaaa
    12541 aaccaaatat ctctactgcc tgcaataatt ccacaaaatc acctgaaatc aaggactcac
    12601 ccttattttc tagtagagaa tctcgaatgg caatcaaatc cttgtagaaa tcattggcgg
    12661 tttcataacg agtcccaact tcttcatcct caatcagata ggttttagtt gcctgaattt
    12721 ttgattgaat atcaaacaag gcacgacggt aaagctcttt ttcgcggtaa atcgagttat
    12781 ccttggattg acgagccatt tctctgactt gcttgctgac gttgacaatg ctagttgaaa
    12841 gagaaaattc acgataaagt tggtaaatct ttttatcata gtagttcatg atgacttcac
    12901 attgagtgag tgcagactgc ttcaaggtct ctgctgtaac aaatggattt ccatcacggt
    12961 caccacctat ccacataccc atggtgattg gtttagcctg ttttaaattc agaccatgcg
    13021 cttgcgctaa gcgcttatac tccgtcgtca aatgaggtac agctttcaaa aaggagctgt
    13081 tataatattc catagcattc gtgatttcgt tagtcacttt taattttttc tcacgaatca
    13141 tgtctgtctg catgataatt tcgatgtaac gacgcaaatc attgtaccat ttatctttat
    13201 tgatcaaccc caacttaaca tcacggtatt tacgcaaaag actatgaata tgatttgtta
    13261 aatccaacat acttttgcgt tgcacttgtg ttggatgggc tgtcaaaaca gggacaacat
    13321 tcaagtgttc taggatctca acggcatttt ccttttctgc taccaattta atcgttgtag
    13381 ataatttacc taaatagtcc tgatcaatat tattttgatg attgatttca taagctaaat
    13441 ccacatcctc tgaaatatta atcaaaagag gcaagataga gaaatagcgt gaaatataga
    13501 ccatttcatc atttgataag ctagtcacta gacggtttag accttgataa tcttcctgcg
    13561 ttgataattc tttcaactgt attatttttt caaaggtctc tggggcaagc atatttttag
    13621 taatatcttc cagtaattct gttagaatca agacttcttc ttgcacaaca cttttattac
    13681 tataattttc taatttttga agagacataa actatccttt ctttatccta cttcaacaga
    13741 aggttcattg gttgattttc caattctcgg tacaacttag cgcgtttttc actggcatca
    13801 atatttaaga caaaggctac tgccactgat aagactagaa gactatttcc accctgggat
    13861 aagaaaggga aagtcactcc tgtagatgga atcaagcccg aaatccctcc gatattgaca
    13921 aatacctgaa ccaacatcat ccctccgaca ccgagtgcaa ccatggcatt gaaaggattc
    13981 tccgctcgga taccgaccaa gataatccgc aaaatcatga aaaacaagag agctaaaata
    14041 agactggcac caacaaagcc aaattcttca atcacgatag aaaagacaaa gtctgtatga
    14101 gcttctggca aataacctcg tttttcaatc gagtttccaa gacctagacc aaaccaacca
    14161 ccattgacca tggcaaaata agaattagct aactggtgac ctgcatcagc acgatcggca
    14221 aaaggattaa aaaaggcact aaagcgcttg gctacatagc caaatactgg aatttttgaa
    14281 aaggtctcaa caccgattag gctgatagtg gtcaagacaa agacagaagt ggcagatacg
    14341 agcgccagaa tggttgaaaa ccagcgataa gcgattccac taactgtata cataatcaag
    14401 gaaaccaaga ctaaaatagt cgcatttcct aaatcaggga aaattcccaa acttccaatc
    14461 agaactagga gaacgaatcg ccaatcatta aaagcacggg gaagccattg attttgagtc
    14521 aaaacttgaa aatcataagt agctatttct tcttgctgtt tggagaatcg gtgagctaaa
    14581 taccaaataa taatgatttt taagtactca gctggctgaa tagttactcc tgcaaccgaa
    14641 atccaaccgt atgccccgtt tactgaaata ccaataaaac gagccaagaa caataaaagc
    14701 atttctatta atataactaa aatgattagt cgctcatttc tcaaaaaatc tagtctcaat
    14761 ttataaatta aggcaatcag tatcaaacta acaatccaaa agattccttg gtttcgaacc
    14821 aactgcaagg cgctcttgcc ttcttcaatt aaaatagcac tggtggtcga atagaccaca
    14881 atcaagccca aaatagataa aagcaagtag ggaatcaaga tggaataatt taataagtgc
    14941 ctcttactaa tcttcatagt atcaccactc tactaggata cagacaaatc tgttccaaat
    15001 tccgttttat tttctagttt tgattgctat tataccattt tttcagaaag aaaaaaactc
    15061 ttaccttaca atccttcgaa ttttactgtt tttacagaat taaggcagaa tttgaaatcg
    15121 taaaaccatt ttgaataaga gttgcttcaa cccattgact ccgacgaatt aagttgcttt
    15181 cgtgaatgcc aaaatcagcc gcaatttgtt cataagtgct ctattctcgc atgtattgaa
    15241 tagttaccat aaggagatcg tctaggctta atttgctttt tcgtccacct tttgcgcgtt
    15301 tacgttgata agctgttttt aacacagcta acatctcttc aaaagtcgtg cgctgaacac
    15361 ctacaaggat cttgaaacgg ctatcgctta attgtttact tgcttcatca tccatagttt
    15421 tattgtattg tatttttgtt tcgcaggaag tctattattc ctttcgttcc aaggttttta
    15481 cgcttttagc aacaagctta cacactgagc gtttcggact taccatatca tttcgttgga
    15541 gattttatca tcaattttta gattaaaaaa aatttcccaa tcttcacaaa cttctgtagc
    15601 aggaattgta tgaatctttc taagtatctt tgtatctttc ctgcttctac accatatgtg
    15661 tattgttggt tttctgaata agctaaaatg ctatctattg catctcttcc gtctatccat
    15721 tcgtgtaaag aatgtacttc gtcatcgcag agttcaatgc taattggttt acacatcgga
    15781 actccaagag aagctacttt ctccatcata tcaaattcaa atttttttat aatctaactt
    15841 cgacttatca gaaacacgca agaaatattt ttactgattt tgatctgtca cacaatattt
    15901 tttatcatct gaccagcctt tatttatggc tatcttacta ctaaaatcca tcaagcacac
    15961 tccgattcac tttacaacac tttttgcagt tttcactagt aacctatcca gttcgtctac
    16021 acttttattt cctagatcca tttttacatt atgaaagtta gtcaattttc tctatgatgt
    16081 ttccaagttt atccaataca agacaagaat gatcatttag tggaattatg ggaacattaa
    16141 cgagttcttc agctctatcc ttatttgctt tatcattcca ggaatcataa tggacactaa
    16201 ttaaaaactg actaaagagt cctaatccat tttttatctt tatctgatga tctgaattat
    16261 cattaggtga gacatagact ttttctccta atagtagggc tcctgcagaa aatcctataa
    16321 tttttgcctc tttattaagc atatgatcga tataactttt gaactcctga ttaacataag
    16381 tagcaatata tttttccgta tttccaccac cgatgataat tatgtcagca tctagatagc
    16441 tatcaagatc aatctgctca gtgtccagga gcaagtaatc aatgtttaga ttaggaaatt
    16501 gactttgaaa aacttccgtg tactttttca tgtacggttc ccaattttct cgaaaaactg
    16561 taaaaatgat aattctttta atcttggttg ataatacaat tttatcaaca atactatatt
    16621 tcttcattgg gggattccca cccatcaaaa aaatttgttc tttcaagtta acacactttc
    16681 taaagataca taaagtttaa acaagtttat tcacgtttct aaattataat tttttcttaa
    16741 aatgaataat tctatttgcc tcctgaaatc caatattgag atgaaatctt atagaatccg
    16801 tattcgttaa agtacagtca cttgcaaatt ccttacatcc tttatttttc gcacattcct
    16861 cacattttgt acagagattt ttagcaatat cctttaaacg atattcctcg tcaacaataa
    16921 tcccttctaa gaatccaaca ggactatatt tacaaccttc aacataatca aatctgagtg
    16981 aacatagtgc tagacctaca attgtgtcac cttcaacttc agtaaagatt gcagtatttt
    17041 tgccattcgt atatttttta cttcatcaat agcttcttta tcggtcaatt ggggccataa
    17101 ttgtttcatt agtttagccg tttttataaa atcttttgtt attccatcca tattgatgtt
    17161 cctccgcaaa ttacaagtaa atgtttcgaa tcatgctgat actttgttac tcattgattg
    17221 ataccaattt tatactgctt tcaaaaggct catatgtttt atacttaatt acttcgtctt
    17281 taaaattaaa taaaggtgta tccggcttag cattccataa aataatcaac cacattatag
    17341 cattataaag ctgctttgca gtttcattcc acttttcagg ttgtaaattt ccactccctg
    17401 tattgtcata ttcttgtcta ttatttacat gagaatcacc tacaagcgga ttaaaaagag
    17461 gtagcccttt gtgattaatt agttctaaaa aatcccttgc cgcacccatg caacactgaa
    17521 taacttcctt cttgtcatca attttactct cagcgataaa tacataatat tcatcttcaa
    17581 tagttgctcc tgcatcacta tgtttttgtt gatttgctaa cagcttaata tgtgctttag
    17641 gctaaacttc aaaatctcta acaattcttt gtcttgtttc atgtcctcta caattcattt
    17701 aagttctcct ttacaagttt agtaactagc cttattatac tatataagat atgaaatgta
    17761 ccataatctt catcaactag tttttagaaa atcacaaatc catctggaga gatgacaagc
    17821 tgattctcca attcttggca attgcttgct agtgctactg cttctttctc taaaagataa
    17881 tcttctgttg attggttgaa tattgctttg aggatccgtc cttcgtattt ccattccaga
    17941 gctactagat cagatttgat ttcttgaagg ctatacttgc catgcgaaat gattactgac
    18001 gcgtatttcc gaatcttaat cagcctcttc ataaagttca gcatatcatt gtcacttgat
    18061 acacgttccc aaggcataca acgacgacaa tctggatctg gtcctccagt caaggctaac
    18121 tcggttccgt aataaatgca cggtgttcct ttttgtaaaa agagaaaggc taaggctgat
    18181 ttaaccagtt gaacatcttc attggccgtc cacaggattc gctctgtatc atgtgaatcc
    18241 aagagattaa acatgacctc tgaaatctgc tgcttgtaat acatagactc tccattgatt
    18301 tcatcgatga actggtctgt cttcttaatt cctcgtaaga aatagtcctt gatactatca
    18361 gataaaggat aattcatgac ggcatggaac tcatctccat ttagccaagg ctgagatgta
    18421 tgccagactt ctcctaggat ataaagatca ggatttttag ctaaaactgc cttacgaaaa
    18481 tccttccaga actgatggtc aatctcatta gccacatcca aacgccaagc atcgatatta
    18541 aactcttcaa tccaataagt cgcaaccttt aaaagataat tcttgacctc tggattggct
    18601 gtatttagct taggcatata gtcctcgaaa ccaaaaacat gatagggtaa gtctctctta
    18661 ttaactagct tttcagttgt cactgggaat tgttgaatat ggaaccaatc cttataagca
    18721 gactgttcac catttttgac gacatttttc cattgaagag attgcgaacc aatatgatta
    18781 aataccgcat ccagcatgac tttcatgcca cgatgatgcg cttgatccac cagttcccga
    18841 aaggtctcct tgtctccaaa atgacggtca atttcaaagt aatctgtcgt attgtacttg
    18901 tgattgcttg tagattcaaa gatgggacaa agatatagtc cagtaatacc caagtcttgc
    18961 aagtaatcca tatgatcaat aatcccctgt aaatcaccac caaagaaatc atcgctctta
    19021 ggtgtgacag atgaatccca gtctaaagtc ccttctgggt ttaatagagc attgccattg
    19081 gcaaatcttt caggaaatat ctgataccat accgtatttg aaacccagtc aggaatcttg
    19141 caggcatcaa tctcatgaag ataaggcaac ttaaatccat tcccaattgc atgaagattt
    19201 tctagagaat tttccacaca ccctttatcg ccatacaaaa tattttgacc ttctgtatct
    19261 ctgagctcaa agagatactg gatacgtgca aagtcaactg acacttcaac ctgccaatgg
    19321 tcaaataagg taccagaagt tatcttgacc atttcttttg tatcctgata aaactcctcc
    19381 ataaagataa aagggtcccc atagtgcaag ttgatgcttt caatgtcccc tttcttagtt
    19441 cgaattcgaa tatggagttt cttatcctta taaagatagg catactccga ctcaggccta
    19501 tggtaaatag cactcaattc catcaatttg tctcctaaag tattcattca aattttacgc
    19561 aaacgttttc aaactcaata aatccagtat actatttttg tgaactattt gcaaggaatg
    19621 tccgttgtta aaggaactat cttttaaagt atacaaaaag ccttaaaaaa ggctttctct
    19681 atatactaat cctctttcgg ttttgaaaat acttctactc gctgagctag gactttgatt
    19741 tctacaggta ggttatctga tttatctccg tcaacatctg actccaattc actatctgaa
    19801 gagattttaa tattacgcgc tttgatatac tcgatattat catttgcgac aacatcccct
    19861 tttaataaat caggaatgac ggataatttg gagaatatag aggcatcttt cagaatcaaa
    19921 atgttggcat agccgtcctt gttttcttca aagattttct tatcagcgaa gtaatttgtc
    19981 aaaaggacca aaacatggct agcttcacca acataatttc cattttctgt ctcaacctta
    20041 atgttaaaga cctgatctgt catgacagac ttcatggtat ttacagcata ggttaaaata
    20101 ccgaattttg ttttgtcctc tatttcaaca ttgtgaatcg cctcaggcag agaaccgata
    20161 ctaaagatat aaccaaaata gttgtcattt gctttaccga tatcaatctt attggttaaa
    20221 tcaaaatcca gttcgtcaat tgcgccatcg atgtcttgat tgatttccaa aagttttgta
    20281 atgaggttac ccgtaccgcc tgggataatc cctaacttag gaatgtagtc tctctcatca
    20341 atacctgaaa tgacttcatt gacagttcca tctccaccaa acacaaccac tgcatcatac
    20401 tgctcacgag aagcttcttc agcaaaatgt gttgcatcca gcgctttttc ggtaattttg
    20461 gtttcaacat attcaaagta ttcttttgct ttattctcca gcttttcttt gtaatccaaa
    20521 gccttctcgc caccagaagt agggttgata attaccattg cttttttcat tgattttatc
    20581 cttaatttta aacagaaatg tttacatttc gtcgtatgca agtaaatgta atcctattat
    20641 acaatgaaaa tacagaaaag agaaatctga cgtactggag attaatacgc ttttattcta
    20701 ttttcccatc gcctaactac atcctttaag ggttcatcca agtaagaata ggccttatcc
    20761 ttgatccaat caggaatacc gtaagctgcc tctgctatgc tacaagtgat tgctgcgaga
    20821 gtatcactgt cgccaccaag tgagatggca tttcttatcg catcttcgaa gtctctactt
    20881 tcaagaaagg cgataatggc ttgagggaca gtttcctgac atgtttcgtt aaaacgatag
    20941 ttaggacgga tttcatctaa agtttgagat agattgtaat cgtattcttt ttctatataa
    21001 tccttaattc gtcttttaca ctctgtagga ttgtcgttaa ttgattgctc gtactctcta
    21061 caataaccac caaagtaaaa acgacataga aagatagcat cagctgtagc catagcgcct
    21121 ttgacacctt ctggatgatt atgagttacc tctgcagaaa gactcgtaag tcctctagat
    21181 gatggccaca taccagtttt cgcataaaaa ccacagtcca tgatccaagc acatggagaa
    21241 atacgcatag ctgatccatt cccaaagcta ttataaggct cacggttatc gctgtttagc
    21301 catgcattaa accgagcacc gtaatcagca ttcggataca ttctgccata tttcttcatc
    21361 gcgtcaatga aatcgtcttt ttgtccacca ttcataattg cttctgcaac agcacaggtc
    21421 ataaccgtgt catctgtaaa aaagcagtcc ttccgaaata aaggaaagtc ctttgttttg
    21481 atattgttcc attcgtaaac agaaccgaca atatctccaa taattgctcc aagcatcaga
    21541 ttcctccttg ttcattttga tgctttttat attggttata taccatattt attttagaaa
    21601 acaacatcct gttggatttt aaaaatttca tttttttcaa aatagggttt taccatttct
    21661 ttccacctag ctctatgaaa attgattgat tttaaaggag ataggccata atttcccaat
    21721 gcataaccat catttacttc aacaacaagt gttctgccat cgcgagtaac accgatatct
    21781 agtccataag ctattggcgc atctttccaa catgatatcg cttcatcaat tacacttgca
    21841 tcaaattgtg catgataatc acctgtatag ggtcgaacat ctaatacgcg accatctaac
    21901 acaaaacaac gccattcagc tatgaattct acaacctcac taatccatat aggatagtcg
    21961 aaaggtagac caatacctat taaatcatgg gttccattaa caactcttcc agtaaagact
    22021 tttgaaccag ctttaggctt aataaatttt ccccaattat caggtatatt cacaatctct
    22081 cctaaaatac cagcataaat ctttcgacca taaaactctt taagctcaat aggatagtca
    22141 tgaaccggaa cgtttaagcc catcattttt agtaatgctc tagtctccat tatataatct
    22201 acaactatat cttcacttgt taactctttt atttcagaaa aagattgata taaaacagct
    22261 tcttctcctt gtaagtaggc acctacttga gcattgtatt tattaattga aacctcactt
    22321 ggtaatttac tttgtctaat ataaacaacc atttcatcac tcctatatca ctagtgttac
    22381 accaatttgt aaaaaataat agcaattttg ctcttatttt tttgagtaaa tagcccccat
    22441 aatatcatcg aaataatcaa cggtatttag gagtaattca ataacctggg actttgttag
    22501 tcgcattccc cttctatctc tagcatcttc tactaaattt tcaagtttct ctagattttt
    22561 atcatccaag ctaatcatta ttctattttt atcggttgcc attttcatca cctcaagtta
    22621 attctatcac aggtgtaaca cttgtgtcaa ctggctttta taatacatta gtttaaaagt
    22681 ggagaggatt tttaacacag taactttaaa tctttggtat taaaaaattt tcacaatatt
    22741 tatagaaata aaatctgtct caaatcagtt atcaaatcta gtataaatta tgagcggcta
    22801 ctctaatact ttccctctaa acaagaaaaa gacttacact caagggtttt cttcccccct
    22861 tcgttataac gttttgactc ttttactagc aaaggtatat actcacaagg aactttggtt
    22921 gactattgaa tctctccaac ttcttcttta acatatcctt ctacatcttc aatctctaca
    22981 aacattgggt ctaagtgaca caagaaatgc caaacttcga tccctttttt tctgtaaaga
    23041 atcgcttcac cgtcttcact tccgaaaaag cttctgtcga tttcatatcc gcggctttct
    23101 aagaagtctt ttgctttacg atagttcgtt tctcttgttt cgacataggc tttaacttca
    23161 tggttgttaa cgacatatgc atcaattttt gaatatcctt cgatcactct atcatttttg
    23221 agggataaat ttgaaatctc tttccaaata atgtttacat tttcctcagg atcgaacata
    23281 aatttagata aaggaacaat atttccgtta aaaataattt ccatataatc cggtatgttt
    23341 ttaggattaa aatactccac ttcaaaacca tcttctgttt ccagagtgta tcccgggatt
    23401 tgagctacaa aggctttccc atcttctatg gaatcaaatg ctactaaatc tttagaataa
    23461 tcattttggt acaattccaa tataaccatc gataatctct ccattttcat tatcaggcta
    23521 atgtaaataa gcacgtcacc tgaccaattc aggctctctg tatcatctca tcatatttcc
    23581 tacttacttt acgagtccta tacccagaac acaccttatc gaccttcggt ctcacctcgt
    23641 cgcattggct gaacatctac ttttacttcg ctgatgcttc aactcgtaca agcagtgata
    23701 ccgcctcagc gtgatgcgtc agtgggactc aaaaggtttg gggaaccttt tgaggattaa
    23761 ctacgtttct ctaataaact tacacattca acttgttcat cattatccaa acctatgttg
    23821 agattttctt ctataattgg tagtttaaaa gtaatggatt ttagccattg tccgttagat
    23881 tgtttttctt cataaacttg aatttcagaa atcaaagctg aaattaactg cctacgctct
    23941 acatcattca tgactttata gagcttatca aaatagatca gaaccttata tatgttatct
    24001 cctgtaagtt tttcagcttc aatagtctgt ttctttgctt tcgcatcaat tagtgatgat
    24061 tctaattcat ctattttgtc atacatacga tatagtctat catctaaatc ctgtttcctt
    24121 ctcttatagt gcttatcttc aacatctaaa ttatctattt cctcaattag cttaaacttt
    24181 gtagaatgac tctttctcaa ttccttttgg taattatcta tttctttttc tatttcagag
    24241 gtatccacct tcatgttgat tttttcttgc atcatagaag caaatttcgg attacttact
    24301 atcttgacaa tcacctctgc aacagcatca tctaacaatt cttctctaat ttgcttactg
    24361 aatgtacact tatgacctct tatcatctgc ctatgtttac aaccatagta ataaaaatct
    24421 ttatactttg tgccatcttt ctttttcttg atacacttgt tcccaaacat tcccactcca
    24481 catatcgggc attttacaat tccagaaagc aagtgtgtgc gtgtatcttt tcctttattc
    24541 acatgctcat atttctttgc ttgagatttt agcttaacct gagcagcttg ccaaacttca
    24601 tcggaaacta tagcttcatg tatcccttca gatattagat attcatcttg ttcaacctgc
    24661 ttatattcat ttcttgtacc atgaactttt tctaaagttc ttcttccaaa tgctattttc
    24721 ccattatata caggattctt taatatcttt cttataagac ctgcatcaaa caaaggattc
    24781 ttaccattct gtcttgggat ttttctaatt ccatgattct ctaagtattt agatatccca
    24841 ttggctccta tcgtagtatt tacatactgg tcgaaaatcg ttcttattgc aactgcctct
    24901 tcctcattta taaacagctt gccgtcttca agtttatatc catacggagc aaagccacca
    24961 ttccattttc cttcccttgc tttttgaatg cgatcttcca ttgtttgaat acggatgttt
    25021 tctctttcta tttcagccac agctgataaa acagaaatca ttagtttccc agcatcttta
    25081 gatgaatcaa tgccatcttc aacgcagata agattaactc cataatcctg cattatctga
    25141 agtgtagaaa gaacatcagc ggcatttctt gcaaatcttg ataacttaaa cacaagaaca
    25201 aaagatactc catcttttcc agattttata tcttccatca ttcgattgaa ctgtattcta
    25261 ccttcaatag acttgccaga cttcccggca tcttcatact ctccaacaat ttcataatca
    25321 ttgtaaatag caaaagcttt cattcgtgat ttttgtgcct ctaacgaata cccctctatc
    25381 tgtattgacg tagatactcg tgtatagagg tatactttta ttttt
//
LOCUS       DAVMAF010000024        24948 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMAF010000024 DAVMAF010000000
VERSION     DAVMAF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.024"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(312..1211)
                     /gene="galU"
                     /locus_tag="VYO97_000984"
     CDS             complement(312..1211)
                     /gene="galU"
                     /locus_tag="VYO97_000984"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1753795.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(1233..2249)
                     /locus_tag="VYO97_000985"
     CDS             complement(1233..2249)
                     /locus_tag="VYO97_000985"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1753796.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            2461..2841
                     /locus_tag="VYO97_000986"
     CDS             2461..2841
                     /locus_tag="VYO97_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1753797.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(3015..3440)
                     /locus_tag="VYO97_000987"
     CDS             complement(3015..3440)
                     /locus_tag="VYO97_000987"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1753798.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(3508..4158)
                     /gene="phoU"
                     /locus_tag="VYO97_000988"
     CDS             complement(3508..4158)
                     /gene="phoU"
                     /locus_tag="VYO97_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1753799.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(4173..4925)
                     /gene="pstB"
                     /locus_tag="VYO97_000989"
     CDS             complement(4173..4925)
                     /gene="pstB"
                     /locus_tag="VYO97_000989"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1753800.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(4927..5742)
                     /gene="pstA"
                     /locus_tag="VYO97_000990"
     CDS             complement(4927..5742)
                     /gene="pstA"
                     /locus_tag="VYO97_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1753801.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(5735..6598)
                     /gene="pstC"
                     /locus_tag="VYO97_000991"
     CDS             complement(5735..6598)
                     /gene="pstC"
                     /locus_tag="VYO97_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1753802.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     IYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(6716..7591)
                     /locus_tag="VYO97_000992"
     CDS             complement(6716..7591)
                     /locus_tag="VYO97_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1753803.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(7758..9089)
                     /locus_tag="VYO97_000993"
     CDS             complement(7758..9089)
                     /locus_tag="VYO97_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1753804.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(9090..9797)
                     /locus_tag="VYO97_000994"
     CDS             complement(9090..9797)
                     /locus_tag="VYO97_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753805.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKT
                     PQFIRTIRGYGYKFKEL"
     gene            complement(9881..10366)
                     /locus_tag="VYO97_000995"
     CDS             complement(9881..10366)
                     /locus_tag="VYO97_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1753806.1"
                     /translation="MAPTDKEDLIRTLELAPHVKGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWESTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            10352..10910
                     /locus_tag="VYO97_000996"
                     /pseudo
     CDS             10352..10910
                     /locus_tag="VYO97_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11225..12916)
                     /locus_tag="VYO97_000997"
     CDS             complement(11225..12916)
                     /locus_tag="VYO97_000997"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1753807.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNTQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NEDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNNTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            13052..13516
                     /gene="argR"
                     /locus_tag="VYO97_000998"
     CDS             13052..13516
                     /gene="argR"
                     /locus_tag="VYO97_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1753808.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKL
                     GEASVLANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            13567..16101
                     /gene="mutS"
                     /locus_tag="VYO97_000999"
     CDS             13567..16101
                     /gene="mutS"
                     /locus_tag="VYO97_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1753809.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTVLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLRYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEHISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            16248..17972
                     /gene="patA"
                     /locus_tag="VYO97_001000"
     CDS             16248..17972
                     /gene="patA"
                     /locus_tag="VYO97_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1753810.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELV
                     GSLSFENVTFTYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQD
                     GAIKIGGKDIREVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANI
                     AQASEFIHRMEKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAK
                     SERLVQEALNKDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVY
                     REIYETQK"
     gene            complement(18302..18565)
                     /locus_tag="VYO97_001001"
                     /pseudo
     CDS             complement(18302..18565)
                     /locus_tag="VYO97_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18763..20529
                     /gene="patB"
                     /locus_tag="VYO97_001002"
     CDS             18763..20529
                     /gene="patB"
                     /locus_tag="VYO97_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1753811.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVSDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFK"
     gene            20668..21357
                     /locus_tag="VYO97_001003"
     CDS             20668..21357
                     /locus_tag="VYO97_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1753812.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            21361..21540
                     /locus_tag="VYO97_001004"
     CDS             21361..21540
                     /locus_tag="VYO97_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753813.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            21527..22876
                     /locus_tag="VYO97_001005"
     CDS             21527..22876
                     /locus_tag="VYO97_001005"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1753814.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            23000..24460
                     /locus_tag="VYO97_001006"
     CDS             23000..24460
                     /locus_tag="VYO97_001006"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1753815.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQIKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            24474..24908
                     /locus_tag="VYO97_001007"
     CDS             24474..24908
                     /locus_tag="VYO97_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1753816.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         7413 a         4893 c         5326 g         7316 t
ORIGIN      
        1 atgttcggct cggttgattt aatttaacca tgactactcc aacagttaag tcaaatttaa
       61 aattatttat tattttggaa ccattcgaaa caacatagca aagttaaaat aaggctttgt
      121 ccgtacacaa cttgaaaaag tgcgcaccga ttcggtgctt ttttgtttta ctatattaat
      181 ttattctact cttattttaa tgaaaaaaca aatacaagta tagcgttttc attagttttt
      241 attaaatagg tagattcaag tagagaattt acttaattta tgtgtggcta atctttatat
      301 aaatgatttt gttattcctt ctcagtcaat tcttttccaa gttggatgag gtaattcttc
      361 aaatcatctt tgacttgtgg gtgtttgagg gcgtagtcga tggatgtttt catgaagcca
      421 aacttgtctc cgacatcgta acgagcccct ttgaactcac gagcaaatac acgttgtgtt
      481 ttattgaggg tgtcgattgc atctgtcagc tgaatttcat ttcctgcacc tggagcttgc
      541 ttttcgagaa tctcaaaaat ttcaggcgtg aggaggtagc gtccgataat agcaaggtcg
      601 ctaggagcgt cctctggagc tggtttttca acaaaggttt caacactgta aagaccatct
      661 tttccttcgc cttgcggagc aataaccccg taagcagata cttcgtcatg agggactggc
      721 atgacagcga tagtagacgc gtgggtacgc tcgtagtcat ccatgagttg tttggtaagt
      781 ggaacagcct tttcgtctgt gatatccatc aagtcatcac caagcataac gacaaaaggt
      841 tcatttccga cgaaagcctt ggcttgcaaa acagcatctc cgagaccgcg tggatgagtt
      901 tggcggataa aatgcagacg catgccagtt gtttcatcaa ctagcttcaa aagatctgtt
      961 ttcccttttt ctttgaggtt atattccaat tcgaagtttg aatcaaagtg gtcctcaata
     1021 gaacgttttg atttaccagt gacaactaga atatcttcaa tacctgattt gagagcctct
     1081 tccacgataa actggatagt tggtttgtct acgattggca acatttcttt ggcaagggcc
     1141 ttggttgctg gtaaaaatcg agttcctagt ccagcagcag ggatgactgc ctttctaact
     1201 tttgatgtca taaaaatcct ttctatagag ggttaagacc actcattttc tgctttaaat
     1261 tcattgttca tgatgtcata aatggcatct ttgatattgg ttccgtgata aataacttgg
     1321 taaatagcct gtgtaatggg catatagact ccaagttctt gggctagttc ataggctgct
     1381 cgagtcgttg aaattccttc gattaccatg cccatattag cttctatatc agctagggat
     1441 tctcctcgtc cgagagcatc tccagctctc cagttacgag agtggatgga agttcccgtt
     1501 acgatcaaat ctcccacacc agataagccg ctataggtca atggactggc cccgagtgct
     1561 acccctaggc gggtgatttc tgctaaacct cgagcgatga tggctgcctt agcattatca
     1621 ccaaatccaa gaccatgtaa agctccagca ccgacagcaa taatattttt aagagcacca
     1681 gcagtttcaa ccccgataac atccgtattg gtataaagtc ggaagtagtg attactaaat
     1741 agctcctgaa cgtattgagc tgtttgtaaa tctttagaag cagcagtgat taaagttagg
     1801 tcacgcacaa tggtttcttc cgcatgacta ggccctgaaa caacgacaat atcactgcgg
     1861 agctgttcag gaatttcttc ttcaaggata gttgataatc gtttatggct atcaggttct
     1921 aatccctttg aagcgtgcat gatgatagcc ttatggtcca aggtttgtgc aacttgttgg
     1981 gcaacaagtc gtgtcacttt tgttgggaca acaaacaaaa tcgcatccac atctttcaat
     2041 gtttctgcta agtcggtgta ggcaatgata ttttcatcta gaacgacatc tttaaagtag
     2101 tgcttgttag tatgataggt attaatttca ttgatttgct cgggaagatt tccccaaata
     2161 cgtacctcgt gtccattgtc atttaagact tgtgaaaggg cagttcccca agaaccaggc
     2221 cccaagacgg cgacggtttg tttttccatg atttcctcct tgatagagtt ctttccatta
     2281 ttctatcata ttctagagga ttttgcactt gcattactgg ggtatggtgg aaaaatacac
     2341 aaagaccgag atgaagggat aaactcttag tctttagtta aagtgagaag aaatctgata
     2401 gatagtttga ttcagtatat gcttataata gaggtaaaca actcaggagg ttcttgtagg
     2461 ttgcgagagt ttggcgaaaa aattaaaaga ttacgtttgg ctaaaaaaat cagtcgttca
     2521 gaattttgtg gtgatgagtc tgaattaagt atccgtcaat taattagaat tgaaaatgga
     2581 gaatccagac caacactaac aaagttaaaa tatatcgctg aacgtttggg ggttgaggat
     2641 tacaagttga tgccaagtta tatagagttg gataaggaat acctagaatt gaagtatttc
     2701 ttgatgagga ctcctacata cgaagatgaa actatcgccc aaaagaaaga gagtgttttg
     2761 ataagatttt tgaagagtat tatgataggc tacctgagga agaaagattt atcatcccaa
     2821 attattcata tctggcacta acgaactaca cagttcaaaa attaccagaa aagctagttg
     2881 aaatactgtc cttctggtga ttttgcttaa tttctgatgt tagagacata attgaggaga
     2941 tttgtcttga ttttttccta aaaaagggca gactcctccc ttggtttgat gctcgatttt
     3001 taaaataagg tgacttattc tgttaggcat gttttcccct gccctctagc tctatatgga
     3061 acagggagta tcagatttat ctggcagatt gtatcaaata aggcttgaaa ctgttttgaa
     3121 taggcatata gacttgagaa tttgagaata tgtcgtctag cagtagagac atattttccg
     3181 gcaatatgaa ggaagagacg tcgaaaacgc ttgatagtca aggcctttac ttcatcacca
     3241 gctagctgct ttaaaaagag gtagagatta taagccagac agcccatcat catacgtact
     3301 tcattcttaa tcatggtcga actatctgtc ttatccccaa agaatcctgc tttcctttct
     3361 ttgataaagt tttcagcatt ccctcgttca cgatagaatt gaaacagctc ctcactatct
     3421 acactgctta aattggtcaa atgaactttt ttacaaccat aaaaaagagc acttaatcgt
     3481 actcttctct tttaaggatt agttgaatta attcaaatcc actagttctc ctgtttctag
     3541 gtagactagg cgttcacaaa tgttagcaat gtaatcagcg aagcgttcca gatgccctat
     3601 gatataaagg tattgtgttc cattgggaat tgaggtttct tggtctttca taagtccaat
     3661 gatttccttt gataaggcat aataatattg gtcaatctgt tcatcttttt gagcaatact
     3721 aatagctttt gaggcttggt gcaaaggaaa ggcaaccaat aaatcggata gcatgctgag
     3781 ggataattta cccatttggt gtaactgttc ttcgtcaggg gctagttgat tttcttttag
     3841 ttgcaaaaca gctttagcaa tgcctgccat atggtctccc atacgttcaa ggtctgaaca
     3901 agaagacatg atgctaatca caaatcgaag gtcagacact tgtggctgct gcaaggccaa
     3961 caaacgggca caggtcaatt cgatagcgct ttgaccttgg ttgatagcat gatccttatt
     4021 gataattagc tctgccatct ccttgtcttt ggaggctaag gccagtaagg cttttgaagc
     4081 tgtttcaagg acaagttgcc ctagtcctaa aaaggattgt tctaattcat gcaattctaa
     4141 gtcaaattga tttctcatag gtttttcctt ccttatccga accgtcctga aatatagtct
     4201 tctgtgcgct gatcttttgg attggtaaac acgtcaacgg tatctccaaa ttcgcaaatt
     4261 tctcctgtta agaaaaaagc agttttatct gaaatacgtg aagcttgttg catgttatgg
     4321 gtaacaatga taatcgtata atcctttttt agttgctgaa tgaggtcttc aatttttaaa
     4381 gtggagatag ggtctaaggc tgaagtcggc tcatccatta acagaatatc aggttctact
     4441 gctaaagctc gcgcaatgca aaggcgttgc tgctgaccgc cagataagga catggcactc
     4501 tttttaagat catctttgac ttcttcccaa atggctgccc cttttaaaga tttctccact
     4561 aaggcatcta attgtttttt gtctcgaata ccatgtgtcc ttggaccata agccacgtta
     4621 tcatagatag acatggcaaa gggattaggc tgttgaaaaa ccatccctac acgcttacgt
     4681 agctgattaa ggttgaattt gctactataa atatcttgct catctaagag gacttggcct
     4741 tcaatatggc aagaaggaac caaatcgttc atccggttaa gggtttttag aaaagttgat
     4801 ttaccacaac cagatgggcc tatcaaggca gtaatctgtc tttctggtaa ttgaatcgaa
     4861 atatttttta aggcttgaaa atccccgtaa aataagtcta ggtgtctgac tgaaaatgtt
     4921 cccatactag gaagctcctt tcacaagttt tcgagataat aagcttgata gagtatttat
     4981 cattaaaaca gtaataatca aaatcacacc ggtagcatag gcttcattga catgtagccc
     5041 ctcacttgac agcatataca tatgcagggc tagagaacga cctgaagaca tgagactact
     5101 tggcgtattg gtagaggtac ctaatgtata catgagggca gctgtctcac caacgatacg
     5161 gccaatagct agtatcactc cagctaaaat acctggcatg gcaactggta gaacaattct
     5221 aaaaacagtc cgtaacttac ctgccccaag tccataactt gcttgacgca tgctatcact
     5281 aacagataaa agggcttctt ctgttgagcg aataatgact ggcaacacca tgatgactga
     5341 ggttaagatt cctgataaca gagagtattg aaaacctaag aagactacaa agaagagcat
     5401 gccaaacaga ccaaaaacaa tggaaggaat cccagacaag gtatctgagg ccaatcgcat
     5461 gattttaaca caaagggaat ctttttttgt atattccaca agataaaaac cagcaaaaat
     5521 ccctatgggc aaggctaaaa gaagagcacc aaagaccaga ataacggtgg aaataatcgc
     5581 tggcataagg gaaatgttct cagaagtata agtccaagaa aagagggata gacttagatg
     5641 aggtaagcct ttgatgagga taaaaccaat gattaaaaag agagagccaa aggttaaagc
     5701 tgaaaaacaa taaacgagaa gttttagcag gtatttactc ataagatgat tttcctttca
     5761 agtaggcaaa gtaggcatta atcaagagaa taaggaaaaa gagaactgct gaggttgcaa
     5821 taagggcttc cctatgctga cctgatgcgt aagccatttc cagaacaata ttggttgtta
     5881 aggttctggt tcctgaaaag agtccacttg gaataatcgg ctggttgcct gccaccaaaa
     5941 taactgccat ggtttcacct actgcgcgac cgattcctaa aataactgct gataaaatac
     6001 cagatctcgc agctggcaag atgacactaa aaatactccg ttcatgacta gctcctagag
     6061 ccaagctacc agaataatac gttttgggaa ctgttcggat agcagattct gacaaactga
     6121 taatggttgg caaaatcatt attcctaata gtaacgaagc ggttaggaca ctcatgccat
     6181 ttcctaaaaa gcttctaatc caaggcacca ataattgtag gccgaaaaaa ccataaacaa
     6241 tagatggaat ggccgccatc aagttgatag ctgattttaa gaagccatag atgggctttg
     6301 gacaataata aaccataaac accgatgtca agatgcctgt tggcacccca atcacaatcg
     6361 ctcctaaggt aattaataag gaaccaacga tcattggtaa aataccatag cttgccggaa
     6421 tgttcgttgg cgaccaatca ctgcctaata aaaaacgggc aaagccgtag ttagccatga
     6481 aaggtaagcc attactaaaa ataaagaaac agattagcaa aatagctaca acagctactg
     6541 ttgcactcat gaaaaaaatt gccctaaaaa ctgcttcttt gaaggcttgt tttatcacat
     6601 cttgtccttt ctagtgaaga aagtaaggga gatacgacac ctccctactt gccttcttta
     6661 tcttattgta cgatgaaacg tctgcatctc tttagagatt tatggagcaa acattttatt
     6721 taatcttgtc ccaggtggtt aatttgccac taaaaacgtc tgcaagttca gccatactga
     6781 cttggcttgc cttattgtca ttattgacca caacagcaat accgtctaaa gcaatagcat
     6841 catgggtgag actcttacct tcttcaggag ttaattccct agaaaccata ccaatatcag
     6901 cggttttctc cttaacagcg gtaatacctg ctgaagaccc attagaggta atatcaatcg
     6961 taacttctgg attttctttt ttataagctt ctgctaattt ttccattaaa gaagatactg
     7021 aagtggaacc tacaacagac aacttgcctg ataagtgttg gcttgtatat tctgtggttt
     7081 cggttttagc ttcaataaat ttattatctg tgaccacttg ttgaccttgt ttggagtgga
     7141 taaagctgat aaaatcttga cctagcttgg aaagattaga ggaccaaaca atgttgaagg
     7201 gacgttgaag agggtattca ccatctaaaa ctgtgtctcg actagccttg acaccatcaa
     7261 tctctaaagc cttgacagat ttcgttaaag atcccaagga gatgtagccg atagcattag
     7321 cattcccttg aactgctgag agaacacctt ctgtactatt ttgaatcaca gctgttttgg
     7381 cagtgttgtc aattttttta tcaccgtctt ttttgagaat ccctgtgatt tctgtgaagg
     7441 caccccgtgt tccagagcca ttttctcgtg aaatcacctc aatcgttcct gaagctgact
     7501 gtttggaagc agctgactga ttgccacagg caacaagccc aaatcctgat aagccaatgg
     7561 ctgcaagagt aagcattttt ttgaatttca taataatcac ctttatctct atgtattttt
     7621 cttgtgtagg cttactacat ttatagtcta acaagtcttt gtaaaggttt atccctgatt
     7681 catgtaaaga ttgtgtaaag aatcaaaaaa agccactttt gaaaaatggc tgctcctaaa
     7741 aatagcttta aaaattatta gtcctgtgcg aaagattggt taggaagaaa aatcgtgaag
     7801 caactgcctc tgccaagctg actcgtcacc gtgacttggc cacctaataa ttgactgagt
     7861 tctttgacaa tggcaaggcc aagaccagtg ccaccagttt gtctgcttcg acctttatta
     7921 actcggtaaa aacgttcaaa aatacgatcc tgctctaatt gactaatacc aatccctgta
     7981 tctgatacag aaatcttaat gccttcgttc accttttggg tcttgacctc aattttcccc
     8041 ccttgttcag tgtaacggat ggcattggat aaaagattga gtaagatttg ggaaagtaat
     8101 tgactatctg atacgagggt gacatcatct ggcacctgca cctttagctg taaatccttc
     8161 ttcttgagct gaggttgcaa gctttgagtc aaatcctgta caaattctgc caaagaaagg
     8221 gtcgtccatt gtataggcat ttgttgagcc ttagataagg taagaagatg ctcaacaata
     8281 tgctcaagac gcaaactttc tttgtaaata atgtctagaa agtcatcctt gagcgcttct
     8341 tcttcagctg acatcccctt aatggtttca gcaaagccct taatcgaagt aactggtgtc
     8401 ctcaattcat gggaggcatt tgagacaaag gctaaattta acttttcata agttctaatc
     8461 gttgttaaat catatagcaa gacgagcaca gcttccacag attgggtggg gctaaaaacg
     8521 ggaactgctg tcacttctaa aatcaagtca ccctcatgaa acccacttac ttcttgtttt
     8581 aaccttgttt tttgatcaaa ggcttggtga actaaatttc gaatatccat ccgtttgagg
     8641 tcatcaagtg aacttatgtc gccgtccaca tcgggaaaat aatgaggcag agagcgactg
     8701 gataataaca tctgaccttg agcggaaact aaaaacgtcc ccatggttag gtgcgacaga
     8761 agaacctcca ttgtttcggc taggtccttg tattgctgat cctgttggga gactttggtt
     8821 tttagggcag acacatactg agccaaagac tttaagtctt cttgcccttt ttctaaaaag
     8881 tattcactac tggtcaagag aggttggtgc aaggtctcaa aagcaacttc ccatttccaa
     8941 aggcaaaaga gccagtagcc accttgtccc aaagaaagtg ctagaagaaa gagaccgatg
     9001 cctttactga tccaagttaa tgccatccct gcaatcagaa tgaggctaac acttagattg
     9061 actagccaaa attgaaggta gcgtttcatc tataactcct tgaacttata accataaccc
     9121 cgaatggttc gaataaattg aggggtttta ggattgtctt caattttttc cctcaactta
     9181 ccaatatgaa cgtccaccaa acgtgtttcc tgcccaaagt cataccccca gatacgttcc
     9241 aaaagacgct ctctagtcag tgtcatgttg ggatgtttca taagatagag caagagttca
     9301 aattcttttg gggtcaaact cagtaactta ttcgccttgt agacttcatg acgctcaggg
     9361 tatactttca aggtcccaaa tagccaagaa tcgtcagcga tattatctga atcatctcct
     9421 tcttgttctc ctttagttcg cctgaggaca gccttgacac gcgccagcaa ttctctaggg
     9481 ctaaaaggct tggtcaggta gtcatcagcc cctaattcca aggccaaaac cttatcaaat
     9541 tcatcacttt tcgcagaaac catcataatt ggagttttga cgcctttggc tctcagccgc
     9601 ttacaaactt ccatgccatc taattgtggt aacatgatat caagcaagat aaaatcaaag
     9661 ggttctgttt ctgccaaagc taaggccttc cgtccatttg ttaccaattg agtagaaaag
     9721 ccttccttac ttaaatggta gtcaagcaat ttcagaatgt gttcttcatc atccactaat
     9781 aagacttgtt ttgtcatcta ttatctccta ttggtaacat tataacacaa ttatcagaaa
     9841 tcctaacatt gctaaatcag attaaatttg cttatcaaga ctagtatctg gtcaaacgct
     9901 caatcatctc cttgtgctct ggataggtcg ccagtagatc taccctttca aataattcaa
     9961 aatcctcaaa ttcaaaacca ggagcaacaa gacaagaaac cagagcatca tccttatcaa
    10021 ctgttgattc ccaaatagtg cccttaggaa cacagtagtg aagttgttgc cctttggata
    10081 tgtccaggcc taaagtgact gcttcgtagt gaccatctgc tgtaatcatg tgaacagtaa
    10141 gtggggatcc tgcatgaaaa taccagattt catctgctgt caatcggtga aaatgtgaag
    10201 gattcgtttc ttctaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa
    10261 ggtttatagt gtctgaagct ttttttgttt gtctaaaata gccaccttta acatggggag
    10321 ctaactctag agttcttatc aagtcttctt tatccgtcgg agccaatggg ttgaagtaac
    10381 tcttgttcaa agtggtttta cgatttcaag aactcctctc agttctgagg acacggtaat
    10441 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa acaattagcg aatgattctg
    10501 gtaaaaagaa atgccacgct atgaaggctc aagcgattgt cacaagtcaa ggaagaatta
    10561 tttctttgga tatcactgtg aactattgtc atgatatgaa gttgttcaaa atgagtcgcg
    10621 gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga
    10681 tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccacta acagttgaag
    10741 ataaagccta taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca
    10801 aagtaaaaac gtttaaaatg atttcaacaa cctatcgaaa tcatcgtaaa cacttcggat
    10861 tacgaatgaa tttgattgct ggcattatca atcatgaact aggattctag ttttgcagga
    10921 agtctattat ttggttaggt gaattagtga agcgtttagg caagtgtctc tggttacgac
    10981 gtcatggcct ctaaatcgat tatatttagg ggtcatgact agtgaagcag ttagctagtt
    11041 cgcatataag cggctagcgt ctaacaatta ggaactttag ttccaataac tttaagatta
    11101 cgacgtttta ggacataaat cgatcatatt tatgtcctaa aactagtgaa gcgcctagcc
    11161 aaagtccgaa taggatttgg cgttagttac ttagattgct ttgcaatcaa gtaactttgg
    11221 cgatttacat cttctctggc gcttctactc caagcaagcg aagggcttct ttgagaacga
    11281 ctgcggttgc gtagctgagg gctagacggc tgtcgcgttc tgggctttca tccaagatac
    11341 gtgtatgtgc atagtatttg ttaaaggatt gagccaggct aattgcaaat ttagcaatga
    11401 tagaaggttc aaagttatct gccgcacggt tgataatacg tgggaagtct tgaatgagtt
    11461 taatgatttc ccagctttca gtattattca agctatagtt gccagctgtt tctggtttga
    11521 aatcggcttt gcgtaagata gattggatac gagcgtaggc atattgaacg taaggtccag
    11581 tttcaccctc gaaggatacc atagcctcta ggtcgaagtc gtatccattt gtacggtcgg
    11641 ttttgaggtc atagaattta atggctccaa ccccaacagc atgtgctacc tgatctttat
    11701 tttccaattc aggattttta gcctcgattt ggaccttggc acggctaaca gcctctgcaa
    11761 cagtaggctc tagcaagatg acattccctt tacgagtaga gagcttcttc ccttcttttg
    11821 taaccaagcc aaaaggaacg tgagtaatgt cgtcactcca gtcgtagccc atctcttgca
    11881 agacagcttt gagctgttta aagtgggcag attgttcttg accaacgaca tagatagatt
    11941 tagcaaattg gtattcgttt ttacggtaaa gggctgcagc caagtcacgt gtgatataga
    12001 gagttgcacc atcagacttc ttgatgaggg ctggatgttc aattccgtat ttctcaagat
    12061 tcacaacttg ggcaccttct gattcaagaa gtagtccttt ttcagaaaga atgtctacaa
    12121 ctgcatccat cttatcattg tagaaggctt ctccgttata gctgtcaaat tcaaccttca
    12181 attcattgta aaggcggtta aattccacta aactttcatc gcggaaccat tgccaaagag
    12241 cgagagcttc ctcatcttca ttttcaagtt tacggaacca ttcgcgcgct tcttcatcca
    12301 agctagggtc attttcagct tcagcgttga tgcggacata gagtttaagg agttcatcga
    12361 ttggatgagc ttttacagct tcttcgtcgc cccatttttt gtaggcaaca atcaacatcc
    12421 caaattgttt accccagtct cccaaatggt tgaccttgac cgtttgataa ccgatttttt
    12481 ggaaaatatg tgacaagcta tctccgataa cagttgaacg caggtggcca atagaaaatg
    12541 gtttagcgat attcggacta gacatgtcga taacaacatt ttcttgtttg ccaatatttt
    12601 ggtcagcata gtgttctttt tcagtggtaa cagcttgcaa tacttgagca gaaatggcag
    12661 atttatcaag gaaaaagtta acgtaaggtc ctgttgcgac aactttttca aaggcttggg
    12721 tgttcatttt ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt tcgactttgg
    12781 caagagaaaa agcagggaaa gcgatgtcac ccatttctga gtttttaggg gtttccagta
    12841 actttaaaat agcctcttgg tccaggctat caatgatgct agccaattcg ctagcaatca
    12901 attcttttgt attcattaag agctcctttt tggacttttc tactatttta tcacaatttt
    12961 aaagaaagaa gaaaaaattt ttgaaatctc ctgttttttt ggtataatat ggttataaat
    13021 atagttataa atatacacgc aagaggattt tatgagaaaa agagatcgtc atcagttaat
    13081 aaaaaaaatg attactgagg agaaattaag tacacaaaaa gaaattcaag atcggttgga
    13141 ggcgcacaat gtttgtgtga cgcagacaac cttgtctcgt gatttgcgcg aaatcggctt
    13201 gaccaaggtc aagaaaaatg ataaggtcaa gaaaaatgat atggtgtatt atgtactagt
    13261 aaatgagaca gaaaagattg atttggtgga atttttgtct catcatttag aaggtgttgc
    13321 aagagcagag tttaccttgg tgcttcatac caaattggga gaagcctctg ttttggcaaa
    13381 tattgtagat gtaaacaagg atgaatggat tttaggaaca gttgctggtg ccaatacctt
    13441 attggttatt tgtcgagatc agcacgttgc caaactcatg gaagatcgtt tgctagattt
    13501 gatgaaagat aagtaaggtc ttgggagttg ctctcaagac ttatttttga aaaggagaga
    13561 cagaaaatgg cgatagaaaa gctatcaccc ggcatgcaac agtatgtgga tattaaaaag
    13621 caatatccag atgctttttt gctctttcgg atgggtgatt tttatgaatt attttatgag
    13681 gatgcggtca atgctgcgca gattctggaa atttccttaa cgagtcgcaa caagaatgcc
    13741 gacaatccga tccctatggc gggtgttccc tatcattctg cccaacagta tatcgatgtc
    13801 ttgattgagc agggttataa ggtggctatc gcagagcaga tggaagatcc taaacaagca
    13861 gttggggttg ttaaacgaga ggttgttcag gtcattacgc cagggacagt ggtcgatagc
    13921 agtaagccgg acagtcagaa taattttttg gtttccatag accgcgaagg caatcaattt
    13981 ggcctagctt atatggattt ggtgacgggt gacttttatg tgacagttct tttggatttc
    14041 acgctggttt gtggggaaat ccgtaacctc aaggctcgag aagtggtgtt gggttatgac
    14101 ttgtctgagg aagaagaaca aatcctcagc cgccagatga atctggtact ctcttatgaa
    14161 aaagaaagct ttgaagacct tcatttattg gatttgcgat tggcaacggt ggagcaaacg
    14221 gcatctagta agctgctccg gtatgttcat cggactcaga tgagggaatt gaaccacctc
    14281 aaacctgtta tccgctacga aattaaggat ttcttgcaga tggattatgc gaccaaggct
    14341 agtctggatt tggttgagaa tgctcgctca ggtaagaaac aaggcagtct tttctggctt
    14401 ttggatgaaa ccaaaacggc tatggggatg cgtctcttgc gttcttggat tcatcgcccc
    14461 ttgattgata aggaacgaat cgtccaacgt caagaagtag tgcaggtctt tctcgaccat
    14521 ttctttgagc gtagtgactt gacagacagt ctcaagggtg tttatgacat tgagcgcttg
    14581 gctagtcgtg tttcttttgg caaaaccaat ccaaaggatc tcttgcagtt ggcgactacc
    14641 ttgtctagtg tgccacggat tcgtgcgatt ttagaaggga tggagcaacc tgctctagcc
    14701 tatctcatcg cacaactgga tgcaatccct gagttggaga gtttgattag cgcagcgatt
    14761 gctcctgaag ctcctcatgt gattacagat gggggaatta tccggactgg atttgatgag
    14821 actttagaca agtatcgttg cgttctcaga gaagggacta gctggattgc tgagattgag
    14881 gctaaggagc gagaaaactc tggtatcagc acgctcaaga ttgactacaa taaaaaggat
    14941 ggctactatt ttcatgtgac caattcgcaa ctaggaaatg tgccagccca ctttttccgc
    15001 aaggcgacgc tgaaaaactc agaacgcttt ggaaccgaag aattagcccg tatcgaggga
    15061 gatatgcttg aggcgcgtga gaagtcagcc aacctcgaat acgaaatatt tatgcgcatt
    15121 cgtgaagagg tcggcaagta catccagcgt ttacaagctc tagcccaagg aattgcgacg
    15181 gttgatgtct tacagagtct ggcggttgtg gctgaaaccc agcatttgat tcgacctgag
    15241 tttggtgacg attcacaaat tgatatccgg aaagggcgcc atgctgtcgt tgaaaaggtt
    15301 atgggggctc agacctatat tccaaatacg attcagatgg cagaagatac cagtattcaa
    15361 ctgattacag ggccaaacat gagtgggaag tctacctata tgcgtcagtt agccatgacg
    15421 gcggttatgg cccagctggg ttcctatgtt cctgctgaaa gcgcccattt accgattttt
    15481 gatgcgattt ttacccgtat cggagcagca gatgacttgg tttcgggtca gtcgaccttt
    15541 atggtggaga tgatggaggc caataatgcc atttcgcatg cgaccaagaa ctctctcatt
    15601 ctctttgatg aattgggacg tggaactgca acttatgacg ggatggctct tgctcagtcc
    15661 atcatcgaat atatccatga gcacatcgga gctaagaccc tctttgcgac ccactaccat
    15721 gagttgacta gtctggagtc tagtttacaa cacttggtca atgtccacgt ggcaactctg
    15781 gagcaggatg ggcaggttac cttccttcac aagattgaac cgggaccagc tgataaatcc
    15841 tacggtatcc atgttgccaa gattgctggc ttgccagcag accttttagc aagggcggat
    15901 aagattttga ctcagctaga gaatcaagga acagagagtc ctcctcccat gagacaaact
    15961 agtgctgtca ctgaacatat ttcactcttt gatagggcag aagagcatcc tatcctagca
    16021 gaattagcta aactggatgt gtataatatg acacctatgc aggttatgaa tgtcttagta
    16081 gagttaaaac agaaactata aaaccaagac tcactagtta atctagctgt atcaaggaga
    16141 cttctttgac aattctccac ttttttgcta gaataacatc acacaaacag aatgaaaagg
    16201 agctgacgca ttgtcgctcc cttttgtcta ttttttaagg agaaagtatg ctgattcaga
    16261 aaataaaaac ctacaagtgg caggccctgg cttcgctcct gatgacaggc ttgatggttg
    16321 ctagttcact tctgcaaccg cgttatctgc aggaagtctt aggcgccctc cttactggga
    16381 aatatgaagc tatttatagt atcggggctt ggttgattgg tgtggccgta gtcggtctag
    16441 ttgctggtgg actcaatgtt gtcctcgcag cctatattgc ccaaggagtt tcatccgacc
    16501 ttcgggagga tgccttccgt aaaattcaaa ccttttctta tgctgatatt gaacaattta
    16561 atgcgggaaa tctagtcgtt cgaatgacaa atgatatcaa ccagattcag aacgttgtca
    16621 tgatgacctt ccaaattctt ttcagacttc ccctcttgtt catcggttcg tttatcctag
    16681 cggttcaaac cttaccttct ctgtggtggg tgattgttct catggtagtc ttgatttttg
    16741 gtttgactgc tgtcatgatg ggaatgatgg ggcctcgttt tgccaagttt caaacccttc
    16801 ttgagcgcat caatgccatt gccaaggaaa atttacgtgg cgttcgtgtg gtcaagtcct
    16861 ttgtccaaga aaaagagcaa tttgctaagt ttacagaggt ctcagacgag cttcttggtc
    16921 aaaaccttta cattggttat gccttttcag tagtggaacc ctttatgatg ttggttggtt
    16981 acggggcggt cttcctctct atttggctgg tcgcgggaat ggttcagtcg gatccgtctg
    17041 ttgttggttc catcgcttct tttgttaatt acctaagcca gattatcttt accattgtta
    17101 tggttggatt tttgggaaat tctgttgtta tggttggatt tttgggaaat tctgtcagcc
    17161 gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct
    17221 tcaaggatat cccagatgaa gagttggttg gaagtcttag ctttgaaaat gtgaccttta
    17281 cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc
    17341 aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct caattgattc
    17401 cacgtctctt tgatccacag gacggggcca ttaaaatcgg tggcaaggat attcgagaag
    17461 tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta
    17521 gtggaacgat tgcagataac ttgagacagg ggaaggggaa tgctactcta tttgaaatgg
    17581 agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg
    17641 aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga
    17701 ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg acctcagcct
    17761 tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg aaggggacga
    17821 caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc
    17881 taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg
    17941 tttaccgtga aatctatgaa acacagaaat gaaagacaaa ctataagaaa agtcaatagt
    18001 tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca
    18061 aaaagctatt gaaaaagtcc attttttcaa aggtaatcct gtgttaattt cagaaattat
    18121 atcacttttt gttcgtcaaa tggcagctct tttttaggat ataaaacagg gttcggataa
    18181 gtttttttgc aaggtggatg atggctacat tgtaatgttt tccttgttct aatttagtct
    18241 taagataggc cttaaaagca ggcgaaaagc gagggcatgc tttggcagct tgtatgagta
    18301 cctaccgcag atgaggggaa ctccgtttga ccattcttcc tgctaaatca atctgatctg
    18361 actgataaat agaagaatcc agtccagcga aagcttgtaa ttgagcagga ttatcaaagg
    18421 catgaatatt tcgaatctca gctaaaatga ccgcccctaa acgatcccca atcccagtaa
    18481 ccgtcgtgat gaccgagttg aactcagcca tcaagtcatt gacacatgtt tccgccttgt
    18541 caatgagcct cttgtaatgt ttgatgtttt cattacacga gataaaacgt ctatgcgtta
    18601 tcaaactcat taccaattaa aacaaaaagc tgtggttaga tcctttcgga aattgtcaag
    18661 cgattggagg aaatgaacta atccacagcg gcttattcca agtataccac ttgggctttg
    18721 gcagtagcta actgcgctaa atataatata aggaggagta aaatgaagac agttcaattt
    18781 ttttggcatt attttaaggt ctacaagttc tcatttgtag ttgtcatcct gatgattgtt
    18841 ctggcgactt ttgcccaagc cctctttcca gtcttttctg gacaagcggt gacgcagcta
    18901 gccaatttag ttcaagctta tcaaaatggc aatccagaac ttgtatggca aagcctatca
    18961 ggaatcatgg tcaatcttgg cctgctggtt ttggttctat ttatctctag tgtaatatac
    19021 atgtgtctca tgacgcgcgt gattgcagaa tcgaccaacg agatgcgcaa aggcctcttt
    19081 ggtaagcttg ctcagttgac ggtttctttc tttgaccgtc gacaagatgg cgatatcctg
    19141 tctcatttta ccagtgattt ggataatatc ctccaagcct ttaacgaaag cttgattcag
    19201 gtcatgagca atattgtttt atacattggt ctgattcttg tcatgttttc gagaaatgtg
    19261 acgctggctc tcatcaccat tgccagcacc ccattggctt tccttatgct gattttcatc
    19321 gtgaaaatgg cacgcaaata caccaacctc cagcagaaag aggtagggaa gctcaacgcc
    19381 tatatggatg agagcatctc aggccaaaaa gccgtgattg tgcaaggaat tcaagaggat
    19441 atgatggcag gatttcttga acaaaatgag cgcgtgcgca aggcaacctt taaaggaaga
    19501 atgttctcag gaattctttt ccctgtcatg aatgggatga gcctgattaa tacagccatc
    19561 gtcatctttg ctggttcggc tgtacttttg aatgataagt ctattgaaac aagtacagcc
    19621 ctaggtttga ttgttatgtt tgcacaattt tcacagcagt actaccagcc tattatccaa
    19681 gttgcagcga gttggggaag ccttcagttg gcctttactg gagctgaacg aattcaggaa
    19741 atgtttgatg cagaggagga aatccgacct gaaaaggctc caaccttcac taagttgcaa
    19801 gaaagtgttg aaatcagtca tatcgatttt tcatacttgc ctgataaacc tattttgaaa
    19861 gatgtcagca tttctgcccc taaaggccag atgacagcag ttgttgggcc gacaggttca
    19921 ggaaaaacga ctattatgaa cctcatcaat cgcttttatg atgttgatgc tggtggtatt
    19981 tattttgatg gtaaagacat tcgtggctat gacttagata gtcttagaag caaggtggga
    20041 attgtattgc aagattcggt cttgtttagc ggaacgatta gagacaatat ccgatttggt
    20101 gtgtcagatg ctagtcagga aatggttgag gtagcagcaa aagcaaccca cattcacgac
    20161 tatatcgaaa gtttgcctga taagtacgat actcttattg atgatgacca gagcatcttt
    20221 tcaacagggc agaagcaatt gatttcaatc gctcgaaccc tgatgacaga tccagaagtt
    20281 ctcattctcg atgaagcaac ttcaaacgta gatacggtga cagaaagcaa gattcagcat
    20341 gccatggagg tggttgtagc aggtagaact agtttcgtca ttgcccaccg cttgaaaacc
    20401 attctcaatg cagatcagat tattgtcctt aaagatggag aagtcattga acgtggtaac
    20461 caccatgaac ttttgaagct aggtggcttt tattcagaac tctatcacaa tcaatttgtt
    20521 ttcaaataag aaagaagttg tcctatgtgg gcagcttttt cttgtccata aaaaatgttt
    20581 atcacagcct taaaaaaaac atattagacg aaagtcattt tgagtgatat gataggacta
    20641 tcgttagcat tcgaaaggag aggcatcatg gctagaacgg ttgtaggagt tgctgcaaat
    20701 ctatgtcccg tagacgcaga aggcaaaatc attcattcat ctgtatcttg tagattcgca
    20761 gagatcattc gtcaagtcgg tggtctccct ttagtcattc ctgttggtga tgagtcagtt
    20821 gtacgtgatt atgtggaaat gattgacaaa ctcattttga caggaggcca aaatgttcat
    20881 cctcagtttt atggagagaa aaagaccgtc gaaagcgatg attacaatct ggtccgtgac
    20941 gaatttgaat tggcactctt gaaggaagcg cttcgtcaga ataaaccaat tatggcaatc
    21001 tgtcgcggtg tccaacttgt caatgttgcc tttggtggaa ccctcaatca agaaatcgaa
    21061 ggtcactggc aaggactacc tttcgggaca tctcactcta ttgagacagt agaaggaagc
    21121 gtggtggcta agctatttgg gaaagaaagt caggtcaact cagtccatcg tcaaagcatt
    21181 aaagatttgg cacctaattt ccgtgtaact gctattgatt caagagacca aactattgaa
    21241 gcgattgagt ctatcgacga gcaccgcatt atcggtttac agtggcatcc agagtttctg
    21301 gttaatgaag aagatggcaa tttagaatta tttgagtatt tattgaatga actgtaacga
    21361 ttggaacatc tggtcgttct ttcttttatt ttttcaaaat ttttagaatg tggcatttta
    21421 cgcaaacgtt tgaattctga taaaaattgg agaaattcga caaaaaaact tgaaaaaaac
    21481 gaaggtaagc gttatgatag aaaagaagaa atattggagg aagaacatgt cacatattaa
    21541 atttgattat tcaaaagttt tagacaaatt tgttgcacca catgaagtgg aatacatgca
    21601 atcacaagta acagcagcag atgaattgat ccgtaaagga actggtgctg gtagcgactt
    21661 cttgggatgg ttggaccttc ctgaaaaata cgatcgcgaa gaatttgacc gcatcttgaa
    21721 agctgctgag caaatcaagt cagacagcga tgttttggtt gtgatcggta ttggtggatc
    21781 ttaccttggt gcaaaagcag caatcgactt cttgaatcac cattttgcta acttgcaaac
    21841 aaaagaagaa cgcaaagctc cacaaatcct ttacgcagga aactcaatct catctactta
    21901 ccttgctgac ttggtagagt atgttgcaga caaagacttc tcagtaaacg taatttctaa
    21961 atcaggtaca acaactgaac cagcgattgc tttccgtgtc tttaaagaac tcttggttaa
    22021 gaaatacggt caagaagaag ctaacaaacg tatctatgca acaactgacc gccaaaaggg
    22081 tgctgttaag gttgaagcag atgctaacgg ttggggaaca tttgttgttc cagatgatat
    22141 cggtggacgc ttctcagtat tgacagccgt tggtttgctt ccaatcgcag catcaggagc
    22201 tgacatcaaa gctcttatgg aaggtgcgaa tgcagctcgc aaagactaca cttcagacaa
    22261 aatctctgaa aacgaagctt accaatacgc agctgttcgt aacatccttt atcgtaaagg
    22321 ctatgcaact gagatcttgg taaactatga gccatcactt caatacttct cagaatggtg
    22381 gaaacaattg gctggtgaat cagaaggaaa agaccaaaaa ggtatctacc caacttcagc
    22441 caacttctca actgacttgc actcacttgg tcaatttatc caagaaggaa ctcgtatcat
    22501 gtttgaaaca gttgtccgtg ttgacaaacc tcgtaaaaac gtgcttattc ctactttgga
    22561 agaagacctt gacggacttg gttaccttca aggaaaagac gttgactttg taaacaaaaa
    22621 agcaactgac ggtgttcttc ttgcccacac agatggtgat gtaccaaaca tgtatgtgac
    22681 tcttccagag caagacgctt tcactcttgg ttacactatc tacttcttcg aattggcaat
    22741 tgccctttca ggttacttga atgctatcaa cccatttgac caaccaggtg ttgaagctta
    22801 taaacgtaac atgtttgccc ttcttggaaa accaggattt gaagaattga gcaaagaact
    22861 taacgcacgt ctataataga agaaaagagt ggtttgccca ctctttttac tctctttatc
    22921 catagaaatt ggactcagcc aagacttgtg atataatata gaaagcaaaa aggcagacgc
    22981 ctagataata ggagaaacta tgtcaaaaga tatccgcgta cgttacgcac caagtccaac
    23041 aggactacta cacatcggaa atgctcgtac agcattgttt aattacttgt atgcgcgcca
    23101 tcatggtgga acatttctca tccgtatcga agatactgac cgtaaacgcc atgtcgagga
    23161 tggtgaacgt tcacaacttg aaaaccttcg ctggttaggc atggattggg atgaaagtcc
    23221 agaatcacat gagaattatc gccagtctga gcgtttggac ttgtatcaaa aatacattga
    23281 ccaactatta gctgaaggaa aagcctataa atcttacgtt acagaagaag agttggcagc
    23341 tgaacgcgaa cgccaagaag tagctggcga aacaccacgc tacatcaatg aataccttgg
    23401 tatgagtgaa gaagaaaaag cagcttacat cgcagaacgt gaagcagcag ggatcatccc
    23461 aactgttcgt ttggctgtca atgagtcagg tatctacaag tggcatgata tggtcaaagg
    23521 cgatatcgaa tttgaaggtg gcaatatcgg tggtgactgg attatccaaa agaaagacgg
    23581 ttacccaact tacaactttg ccgttgccat cgatgaccac gatatgcaaa tctctcatgt
    23641 tatccgtgga gatgaccata ttgctaatac accaaaacag cttatggtct atgaagctct
    23701 tggttgggaa gctccagagt tcggtcacat gaccttgatt atcaactctg agactggtaa
    23761 gaagttatct aaacgtgata ctaatacact tcaatttatc gaagattacc gaaagaaagg
    23821 ttaccttcca gaagcagtct ttaactttat tgctcttctt ggttggaacc caggtggtga
    23881 agatgagatt ttctctcgtg aagaactcat taaacttttt gatgaaaacc gcctcagcaa
    23941 gtcaccagca gcctttgatc agaaaaaact agactggatg agcaatgatt atatcaagaa
    24001 tgcagacctt gaaaccatct ttgaaatggc aaaaccattc ttagaggaag caggccgttt
    24061 gactgacaag gctgaaaaat tagttgagct ctataaacca caaataaaat cagtagatga
    24121 gattatccca ttgacagatc ttttcttctc agatttccca gaattgacag aagcagagcg
    24181 cgaagtcatg acgggtgaaa cagttccaac agttcttgaa gcattcaaag caaaacttga
    24241 agcgatgaca gatgataaat ttgtgacaga aaatatcttc ccacaaatta aagcagtcca
    24301 aaaagaaaca ggtattaaag ggaaaaatct tttcatgcct attcgtatcg cagtttcagg
    24361 cgaaatgcat gggccagaat taccagatac aattttcttg cttggacgtg aaaaatcaat
    24421 tcagcatatc gaaaacatgc taaaagaaat ctctaaataa agaggataca ataatggaca
    24481 tctgggaaaa gatgtacgaa gaagcacaga aactatacaa tccacatgaa gtatcagact
    24541 ttgtttatgc caatcatgtt gtagctgcag tagaagcaga agatggacaa atatttacag
    24601 gattctgtat ggagggaacc tgtggtgttt tccatctctg cgcagaacgg gcggccctct
    24661 tcaatatgta ccaattttca ggacaaacta aggttaagaa agtattagcc tttcgagaca
    24721 aaccacctta tggtggaagt tcagccatgc cttgcggtgc ttgtagagaa ttccttttag
    24781 agcttaatgc tgaaaataaa gatgcagaat tcatgatgga ctataatata agaaaaacag
    24841 ttaaagtcgc agaactaatc ccttattggt ggggaaaaga acgtgcttct aagtttaatg
    24901 agcgatagaa gagtcagtat aattctggct ctttttacta taagttgc
//
LOCUS       DAVMAF010000025        24399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMAF010000025 DAVMAF010000000
VERSION     DAVMAF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.025"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(226..411)
                     /locus_tag="VYO97_001008"
     CDS             complement(226..411)
                     /locus_tag="VYO97_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753817.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(430..843)
                     /locus_tag="VYO97_001009"
     CDS             complement(430..843)
                     /locus_tag="VYO97_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753818.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(974..1621)
                     /locus_tag="VYO97_001010"
     CDS             complement(974..1621)
                     /locus_tag="VYO97_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1753819.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            complement(1798..2448)
                     /locus_tag="VYO97_001011"
     CDS             complement(1798..2448)
                     /locus_tag="VYO97_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1753820.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            2560..3402
                     /locus_tag="VYO97_001012"
     CDS             2560..3402
                     /locus_tag="VYO97_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1753821.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            3478..3915
                     /locus_tag="VYO97_001013"
     CDS             3478..3915
                     /locus_tag="VYO97_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1753822.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            3983..4285
                     /locus_tag="VYO97_001014"
     CDS             3983..4285
                     /locus_tag="VYO97_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753823.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(4376..5449)
                     /locus_tag="VYO97_001015"
     CDS             complement(4376..5449)
                     /locus_tag="VYO97_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1753824.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            5605..6282
                     /locus_tag="VYO97_001016"
     CDS             5605..6282
                     /locus_tag="VYO97_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1753825.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMKVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            6275..7249
                     /locus_tag="VYO97_001017"
     CDS             6275..7249
                     /locus_tag="VYO97_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1753826.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(7298..9241)
                     /gene="thrS"
                     /locus_tag="VYO97_001018"
     CDS             complement(7298..9241)
                     /gene="thrS"
                     /locus_tag="VYO97_001018"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1753827.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(9299..9622)
                     /locus_tag="VYO97_001019"
     CDS             complement(9299..9622)
                     /locus_tag="VYO97_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1753828.1"
                     /translation="MELQELVERSWAIRQTYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            10492..11121
                     /locus_tag="VYO97_001020"
     CDS             10492..11121
                     /locus_tag="VYO97_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1753829.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            11262..11531
                     /gene="rpsO"
                     /locus_tag="VYO97_001021"
     CDS             11262..11531
                     /gene="rpsO"
                     /locus_tag="VYO97_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1753830.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            12346..12960
                     /locus_tag="VYO97_001022"
     CDS             12346..12960
                     /locus_tag="VYO97_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1753831.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(13253..14002)
                     /locus_tag="VYO97_001023"
     CDS             complement(13253..14002)
                     /locus_tag="VYO97_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1753832.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            14135..16471
                     /locus_tag="VYO97_001024"
     CDS             14135..16471
                     /locus_tag="VYO97_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1753833.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKMF
                     GASQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            17000..18463
                     /locus_tag="VYO97_001025"
     CDS             17000..18463
                     /locus_tag="VYO97_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1753834.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            18456..18884
                     /locus_tag="VYO97_001026"
     CDS             18456..18884
                     /locus_tag="VYO97_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753835.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            18862..19311
                     /locus_tag="VYO97_001027"
     CDS             18862..19311
                     /locus_tag="VYO97_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753836.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            19339..19650
                     /locus_tag="VYO97_001028"
     CDS             19339..19650
                     /locus_tag="VYO97_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV1753837.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            19676..20761
                     /locus_tag="VYO97_001029"
     CDS             19676..20761
                     /locus_tag="VYO97_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEV1753838.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILRKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            20772..21467
                     /locus_tag="VYO97_001030"
     CDS             20772..21467
                     /locus_tag="VYO97_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1753839.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            21504..23474
                     /gene="tkt"
                     /locus_tag="VYO97_001031"
     CDS             21504..23474
                     /gene="tkt"
                     /locus_tag="VYO97_001031"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1753840.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            23844..24399
                     /locus_tag="VYO97_001032"
                     /pseudo
     CDS             23844..24399
                     /locus_tag="VYO97_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7172 a         4364 c         5453 g         7410 t
ORIGIN      
        1 taaaatactc caaaaagaaa tcggttcttg atatgcttgg gggaagtctc ttttcaggct
       61 ttggcagatg cgataggaag ggatgagatg tcctaggatg aggagagttc cctgaaggaa
      121 aagtggaata tcacttaaag acaccaagga gagaatcatc aggctatccc ataggagaat
      181 ttgtaaagca aaacgccaga atctcggtct aatctgggaa tagagttaac taaagtggtc
      241 acaaagttgg tttgaaagat aggtcaatag cacaggatga aagaaaatga gccaaccgct
      301 ataaatcaaa atccagtccc aagtaagccc ccttttaaat gtcaaaaatg ccagcatgat
      361 tccatcaatg acaaggagtt gaatggtttt gatgaagatg atttttttca tgctaaaacc
      421 tccttttcct tagagacact cccataaaga gatgtgtttg tcgtaaaagt ctggatagtt
      481 ttctcttagg tagtcagctg tcatataata aaaagtagaa tggcaagcag tgatgattgg
      541 cataagagat gaaaaacgct gagaactaat gaaggctaag agaacaaaaa atagaaaatc
      601 aatggtgaga acccctaaat agtagaagcg aatcttttta ttgatttgag gataaatctc
      661 agcgaattga tgtttgagtc ggacgaccaa gagaaatagc aggagtcctt gagcaaggaa
      721 gaaaattaaa ccagagagca agagccactc aaaagatgtc tcgagtctag tgataaaaaa
      781 tgcaaatagt agcaaatcaa taactgcaat tgctataaaa tggattctat agagtttttt
      841 catgacaaga cctccctctt ttatctaact tcattctact ccaaaagaat gggagttaca
      901 actaaaatga taaaaatagc agaagggaga ttctcttaag ttggctagta ttctttattt
      961 gagtttcctt ctattatcta acttcttcat cattccagac aaataaagct ccgattgcat
     1021 tgaggatata aaagatgtat ttaccgatat tggcgaagtt tccttgaata ccagcttttg
     1081 tcagctgaac gaaattgtaa atcaaccaaa agccccactg agttgttagt tttaatgcat
     1141 tcaaagcatt ggcaatgagg gacagtgcaa aggcaatagt tgttacgtag gcaaggagat
     1201 tcatcttgcc cccatatccg atatagttgg tcacaaaggc aaagaggaag gcgatgatgg
     1261 aaatgatgat ggccgccaat tttacctgtt tttggctcat ttggttgggt ctgccttctt
     1321 gcgaagcttc ccacttcttt atagcaaagg tataaatgag gaaggtgacg ggataggtaa
     1381 tgatggccgc cttatttcca aggatataat caatagcacc ggacaaaatg gtattaacaa
     1441 taccaaagta atttccccat ttgcttaatt tccccgtgaa acgagtggac aacatggaaa
     1501 tcccaacgtt ggttacggaa atcaatccaa agggtacaag agctgtccat gatccccagt
     1561 ctacaaattt atcgaggtgt gagttgaggt aaccagatgc aatcgcaatc ccaacgacca
     1621 aagcaacccc gaagaggtca aactatttag atgtagcaaa aatttttagt gattttttca
     1681 taggttaaac tacctttctt tttttcaaat attctcccac caaatgaaag taaaataaaa
     1741 tgatagaaat aaaaccctga aaataaaact ttcagggttg gtaggggact tgagaaatta
     1801 gttgattttc tcgacataga gttgttttgc aatgtccata ttcacttgta aatcctcgtc
     1861 gttactgagg atagtgaagg tattgctgaa gccatcaaac tgcttgactt ggagctggtc
     1921 accgatgtga agtgagtgct tgtccagata atggagaatg tcaaaactat cgtgcacccg
     1981 agtcaggcgg taggcgccag cttccttgat atcagctagt gggaggttat tgatttcaac
     2041 gagtagttct cccttggcag gaatagttcc tccgtggggg caggttttag ggaaacctag
     2101 cagtttatct agtctttcca cgaacaggtc agagacagtg tgttccaaga cctcagcttc
     2161 ctcgtgaatc tggtcacttg tatagtctaa atgatgaact agaaaaactt caatcaagcg
     2221 gtgcttacga tagagctcag agaccagttt gagaccgagg tcagtcagta gatagccaca
     2281 ttccttgtcc tttaggatga gattttcact tttcattcgt ttgatcattt cagttacggc
     2341 agggggagag acttgcatgc gagccgcaat ttccttgttg gtaatcttat gcaggtctat
     2401 gccaatttca taaatacatt ttagatagtc ttctttgttt ggggtcattc gtttcctctt
     2461 gtctaatatc tttatctagt atatcaaaaa aagctagatt tctctagctg tgacttttta
     2521 tgttatactt attgcaagag aaaaaacgag gagatggata tgacgaaaat agctcttctt
     2581 tcagatattc atggaaatac caccgccttg gaggctgttt tggcagatgc tcagcagcta
     2641 ggagtggatg aatactggct tttgggagat attctcatgc cagggacagg acgtagaagg
     2701 attttggact tgttggatca actaccgatt acggctagag ttttgggaaa ctgggaagac
     2761 agtctttggc atggtgtccg taaggaattg gacagtactc gccccagtca acgctatctc
     2821 ttgcgccagt gtcagtatgt tttagaggaa atttccctag aagaaattga agtgctccac
     2881 aatcaacctc ttcaaattca tcgtcagttt ggggatttga cggtgggaat tagccatcat
     2941 ctgcctgata agaactgggg gcgagagttg attcatactg gcaaacaaga ggagtttgac
     3001 cgcttggtga ctcatcctcc ctgtgatatt gctgtttatg gacatattca ccagcagttg
     3061 cttcgttacg ggactggtgg gcaattgatt gtcaatccgg gttcgattgg ccaacctttc
     3121 tttttggacg ctcagctacg gaaggacttg cgggcccagt atatgatttt ggagtttgat
     3181 gacaaaggcc tggtagatat ggacttccga cgggtagact acgatgtggc agcggaattg
     3241 cagctggcta aagacctgag acttccctat tttgaggttt actatgaaag tctggtcaat
     3301 gggatccacc atactcatca tcaggaattt ttgagagaat tggctcagaa agagggctgc
     3361 gaccgggagt tagacgactg gttgaaaagt ggtaacgatt gacattacaa ctaaaaaggg
     3421 tataataata gaaaaagaag agtagaggca gggtagcctc gtaaaaagga gaagaggatg
     3481 caaattccaa gtagatttac cattgcgact catatgctga taatcattgc cctcgagggg
     3541 aaggaaagca aggtgaccag tgattttctg gctgctagtg tcggggtcaa tcctgtcatt
     3601 atcagaaaga tcttgtccca gttgaagaag gcagagctga tttcagtagc gcgtggaacg
     3661 ggcggaacag agattgtcaa ggaccttaag gatattagtc ttttagatgt ttatcaggcg
     3721 gtcgaatgtc ttggtaagac aggtcaactc ttcagtttcc atgacaatcc gaatccaaat
     3781 tgccctgtag gagctcatat tcatgatgtt ttggatcaaa aattggagag aattcagttg
     3841 actatggagg cagaacttgg tcaaaccagt ctagaaaaag tcgtggccga tgcagagagt
     3901 cagatgaagg attaagaggg ataaatgccc tcttttttct ttggataatt atgataaaat
     3961 gtagctatta aaggagtaag aaatgtatct cgttatagga attgtattag cctttatagt
     4021 gtcattttgg aaggataata gaagtttgtg gaatccagta ttatttttat tatccctcat
     4081 ttcaagttat ttttatctat cctacctttt ttataaaaat ggatacgaga atgttcagtt
     4141 agctttttat gtatttgcct ttgtcttact tccttttttg ctttttctaa gtggcatctt
     4201 tttaatctat aatggggtca ttttgttgaa gcgagaggga cgttccaagc cccattatct
     4261 ctcaatgcta tttgattttt attgactatt agattctatt taaaaagact tagaatgcac
     4321 atgaaagtat gtattctagg tcttttgttt tgtatacatt taattttcaa gagtattatc
     4381 tatttttcca tgcctggtaa cgagtatcaa tcaataactg ggtaaggcga cccaaggttt
     4441 ctctttcttc tggagtgagg gattgagctt ggacaaagag atgatgaatg tattcaagcg
     4501 cctttaagga agccaagtca ttgatagagc taataccagc ctcaagaata gtgccaaaga
     4561 agaggtcgaa gtagagctga agttcttcgt cagggactgt ggccacccat tctttaaagg
     4621 ttgtgtctac ttgctggcta tcactgttgg tcttatccag ttggacgaag tgcttatcct
     4681 caatctgcca actaaaggta tcgtgctggg cgatgccacc cagggcagta ctctgaacga
     4741 tgatttggtg agcaggaatt tccagcatca taccgataat ggaaccttgt ggaatgaata
     4801 tcttgcttct atccattatc ctttgataac ccgcagtctg tgtcaattct tgatggagac
     4861 caggtgcatc aaatgtataa actgctgtga tctgattttg caaactttgc tcaatttggc
     4921 tagcagcata gatagcgaga tttcctccct tggaatgccc agctagaata accttttgct
     4981 taggatgatg ggcaaaaaag ttctttaaat agcgaagggc gtgcttttga gcaggaattt
     5041 ccttcatata ggtcaggtgg aaatcttcct tccagccaat gatactgtca tctgtcccac
     5101 gaaagacaat cagataggta tcgaggctga cacgataagt catagccgca aattgctttt
     5161 gcagttcagg gtcgatgtcg ttgataaaat gggagagttt gcaatttttg aagcgcttgt
     5221 gttgagccaa ttcatctaat aattgaaggc gatttttgct agtaagcatg gtgggatctc
     5281 ttggaacctg aggtgctaga tctaaaagac gttgaggaag tgtggagacc agattatcaa
     5341 aggagaggta ggtgatttct attaaggtta gaatgtctaa ctcattcaag ggaaggtcgt
     5401 aataagaatc atatgcgaca tctttcagat agtcaaaaat attggccata agagctcctt
     5461 tctttttatt tatcttagca aatttccctc aaattagcta gtagcattgc ctgtttgtac
     5521 tggctaaaaa caggctattt caaattcagt ttcagaccat ctggcatgga aaaatctgtt
     5581 ataataatag aaaaggagaa gcgcatgcac aagattttat taatagaaga tgatcaggtc
     5641 attcgtcaac aggtcgggaa aatgctctct gaatggggat ttgaagtggt cctggtagaa
     5701 gactttatga aagttttgag tctatttgtt cagtcggaac ctcatctggt cctcatggat
     5761 attggtttgc ccttgtttaa tggttatcac tggtgtcagg aaatccgcaa gatttccaag
     5821 gtacctatca tgtttctttc ttcgagagac caggctatgg atattgtcat ggcaatcaat
     5881 atgggggcgg atgactttgt gaccaagcct tttgaccagc aggttctttt agctaaggtt
     5941 cagggcttgt tgcgtcgttc ctatgagttt gggcgtgatg agagtttgct ggaatatgct
     6001 ggtgttatcc tcaataccaa atccatggat ttacattatc aagggcaagt cttgaatttg
     6061 accaagaatg aattccagat tttacgcgtg ttatttgagc atgcaggcaa catcgtagca
     6121 cgtgacgacc tgatgcggga actttggaac agtgactttt tcattgatga taataccctc
     6181 tctgtcaatg tggctcgttt gcgtaaaaag ttggaggagc agggattggt aggatttatc
     6241 gagaccaaga aaggaatagg gtacggattg aagcatgctt gattggaaac aattttttct
     6301 agcctatctg cgctcccgta gtcgtctttt tatctatctg ctttctttgg catttcttgt
     6361 cttactcttt cagtttttat ttgccagtct aggaatttac ttcctctact ttttcttctt
     6421 gtgttgcttt gtaaccatat tatttttcac ttgggacata ttggtggaaa cgcaggtcta
     6481 tcgccaggaa cttctctatg gagagaggga agccaagtct cctttggaaa tagctttagc
     6541 agaaaaatta gaagcgcgtg agatggaact ctatcagcag aggtcaaaag cagaaagaaa
     6601 actgacggat ttgctggatt actatacctt gtgggtccat cagataaaga cccccattgc
     6661 agccagtcaa ctcttagttg cagaagtggt cgaccgccaa ctgaagcagc agctagaaca
     6721 ggaaattttc aaaatcgact cctataccaa cctagtttta cagtacctgc gtttagaaag
     6781 tttccatgat gatttgctct taaagcaggt tcaaattgag gacttggtca aggaaataat
     6841 tcgtaaatat gctcttttct ttattcaaaa aggcttaaat gtcaatctac atgaccttga
     6901 taaagaaatc gtgacggata aaaagtggct gctagtggtt attgagcaaa tcatctcaaa
     6961 cagtctcaag tacaccaagg aaggtggtct ggagatttat atggatgacc aagagctttg
     7021 tatcaaagat acgggaatcg ggataaaaaa cagtgatgtc ctccgagtat ttgaacgtgg
     7081 cttttcagga tacaatggcc gtttgaccca gcagtcctct ggacttggcc tttatctatc
     7141 taagaaaatt tctgaagaac tggggcacca gattcgtatc gagtctgagg tcggaaaagg
     7201 aacgacagtg cggattcagt ttgctcaagt gaacttagtc cttgagtaaa aatagatgag
     7261 aaaaagcctc cagattggag gcttttatgc tagactctta tttctcaacg cgtgatttgt
     7321 tggcgatatc agctaggata gcttgaacaa aattatcaac tgagacagtt tgtgtttctt
     7381 tttggccgta gcgacgaacg ttgactgttt cgtcttccat ttctttgtct ccaacaatta
     7441 attggtaagg aatcttgctg gtttgtgaag cacggatctt gaactgcatt ttttcattgc
     7501 gctcatctac gtctgcacgg acaccgcggt cacggagttt cttggccact tcccaagcgt
     7561 agtccacgtg tttttcgtta gatactggga tgagggttac ttggtgtggt gccagccatg
     7621 ttgggaaggc ccccttgtag ttctcaatca agatagctgt gaagcgttcc atagttgaga
     7681 taactccacg gtggatcatg actggacggt gatcttcgcc atcagctccg atgtatttga
     7741 ggtcgaagcg ttctggtaac aaaaaatcaa gttggatagt agaaagggtt tcttcttttc
     7801 caagggcagt tttaatctgg atatccaatt ttggtccgta gaaggctgct tcaccttcgg
     7861 cttcaaagta gtccacgccc atttcatcaa gagctgcacg aagcatggtt tgggcatttt
     7921 cccacatctc atcgttatca aagtacttat gagtatcttg agggtcacga agagagaggc
     7981 ggaagcggta gtcagtcaag ttgaagtctt cataaacatc gataatcaac tgaagggcac
     8041 gttggaattc ttcttggatt tgttctggag taacgaatag gtgaccgtcg ttgagtgaca
     8101 tttcacgtac acgttgaagg ccagtgaggg caccagattt ttcgtaacgg tgcatcatac
     8161 cgatttcagc gatacggatt ggcaattcac ggtaagagtg aacatggtgt ttaaaaactt
     8221 ggatgtggtg cggacagttc attggacgaa ggacaaattc ttccccgtca cccatgtcca
     8281 tggttgggaa catgtcttct tggtaatgat cccagtgacc agaagtcttg taaagctcaa
     8341 cagaagcaag tggtggagtg tagacgtgtt ggtagccaga agccaactct ttgtttacga
     8401 tgtagcgttc caattcacga cggatagtcg caccatttgg caaccagaat ggcaaacctt
     8461 gtcccacttc ttgtgaaatc ataaagaggt caagctcttt accaagttta cggtggtcac
     8521 gttccttagc ttcttcacgc atttgaaggt agtttttcaa gtctttcttg tcaaaccaag
     8581 ctgtaccgta gatacgttgc atcatagcgt tgtcgctgtt tccacgccag tacgcaccag
     8641 ctacatggag aaggtggaag atttggatac gacctgttga tggaacgtga ggtccacggc
     8701 agaggtctac atattcaccc tgacgataga tagtcaaacc gccttcgtct tctgagtgtt
     8761 cttcaatcaa ttccaacttg taagggtcat ttttgaagat ttcacgtgcc tcgtctttag
     8821 tcacttcttc acgaatagat gggaagtttt ctttgacgat tttttgcatt tcttcttcga
     8881 tacgaggaag gtcttcgtta gagatttgac cagctgtgtt gtcagtatcg tagtagaaac
     8941 catcttcgat ggctggacca actcccaagt gaatgtctgg gaaaagacga cgagctgctt
     9001 gggcgaacaa gtgagctgct gagtgacgca agattggaag ggcatcttcg tgatcaggtg
     9061 tcacaatttc gatgcttcca tcttcagtga tagcgcgagt agtgtcgatg agtttgccgt
     9121 tgaatttacc agccaaggct tttttagcta gggaattgct gatagattgg gcaatttcaa
     9181 aagttgttac gccagattcg aattcacgaa cagcgccatc tgggaaagta atgttaatca
     9241 tgatgttctc cttttttatt atttcatagt ttctaagcat tgatttttta tcagcagact
     9301 acttccaagt cctaacgttc aattgctttt ctttatcaga gaggaagttt tccatttccg
     9361 tcagaatgtc tatatccaaa cgttgagaaa gttctaatag ccaccagata ttttctgaaa
     9421 gtttttgttc cagtgtgtag ggtgtttcat catagtagcg tccttgcttt gtcatcacca
     9481 gtcgttggaa atttccaata tcattagata aagccaagag gtcttcttct accgtccact
     9541 tggaatcatg atgcttaact tccagttcgt gataagtttg tcggattgcc caactgcgct
     9601 ccactaattc ttgcaactcc ataattagta tcctaataaa aaaatgcgac atcctcgaaa
     9661 ggacgtcgca acgtggttcc accttcattt atgtacctca aaagagaggg acacctctga
     9721 tcggctctaa cgtggccacc gttttatgtt ttcataaaaa actcaagagt agtatcagtt
     9781 tagacttcct atgcgtttcc agcaaccacg cactctctag tagaaaggga ctaagtgact
     9841 tgtctctatg gattattata gcatgattgt gagatttttc aaggattttg tgaaagtttt
     9901 ttgtttttca ttttttgtag aatatgcttg atgctaatac cagaatccat gcccaccaga
     9961 gcaactgttt catagattag caagtaaatt agtataaact caccaaaggt tatgatagta
    10021 aagtaaaaga gcagtccgtt accaactaac catcaaagtg agaaacggaa acggtagcta
    10081 tagagtaacg atataatcaa gattaccaga ggtgtaaaga agagaagatt gtttatatag
    10141 agttccttga gtgtaagggg gtcagaaaca actagtaaga acaactcata aagtggccaa
    10201 taaaagaata atatgaccag atagtagggg agatgggaat agaatctacg catgataatc
    10261 acctcgtttt aaaaaaacag acaacttaga tctcttgcta tgattattat agcatgttta
    10321 aaacaagaaa gtaaatttgt tgacaaagaa gatttttctt ttcactagaa tcatagttag
    10381 ttttcttggt agaaatattt tgataataat taagcaagac tattgacatt gattattaag
    10441 agtgtcacaa ttattgtaac taaaataatt acaacaagag aggtataaaa aatgaaacta
    10501 gcagttattg cagcaaatgg acaagcgggg aaagcaattg ttgaagaagc agtcaaacga
    10561 ggacatgaag taacggctat tgttcgttct gaaaacaaaa gtcaagctga gtctattatt
    10621 aaaaaagatt tatttgagtt gacaaaagat gatcttactg gatttgatgc tgttatttca
    10681 gcgtttggtg cttatacacc agatacgcta ccgctccaca gcaaatcaat cgaactattt
    10741 aatcagcttc ttgctggaac acagacacgt tttctagttg tagggggagc aggaagtctt
    10801 tatatagatg aaacaaaaac gacacgactt ttagatacgt ctgattttcc tgaagaattt
    10861 aagtcactag caaaagatca agcggatgaa ttggatcttc ttcgtacaaa aaataatctc
    10921 aactggactt ttgtcagtcc tgctgtagat tttattcccg atggtgaaaa aacaggaaac
    10981 tacattctag caggagaaat ttttactact aatgaaaaag gaataagcca gattagttat
    11041 gcagattatg ctattggatt ggtagatgaa cttgaaaaag gtcatcatat aaaagaacgt
    11101 atctctttac ttgaaaaata ataactatga gagtattaat gtttgacaga aaatttaaat
    11161 cttgataaaa tagggattgt cgtaaagaca aataacttct tcttggttac aggcatgcca
    11221 acctgtcact cggatgaagc caaataaaaa ggagaaacat catggcaatc tcaaaagaga
    11281 aaaaaaatga aatcattgca caatatgcac gtcacgaagg tgatacaggt tcagtagagg
    11341 ttcaagttgc tgtccttact tgggaaatca accaccttaa cgaacacatc aaacaacaca
    11401 aaaaagacca cgctacttac cgtggattga tgaaaaaaat cggtcgccgt cgtaacttgc
    11461 ttgcatactt gcgtaaaaac gacgttaacc gttaccgtga attgatcaac tctctaggac
    11521 ttcgtcgcta attcaagata caaaggccgt caaaagcaca aagcaaaaat aggaaaattg
    11581 acgaagaaac ttcagtttct aggagatttt atcttttttg ccaagtgctt aggccgtgtt
    11641 caattgagca tatcttgata ataaagctac tctaaattgg gtagcttttt tgcgttgtca
    11701 ccttacctcg ctatactcaa gtataatctt cggttacggt tcctagcact gtaaggtaaa
    11761 ataaactagc ttttctccct tcggggagat tttttttgtt tcactgaaag tttcgtacga
    11821 tcttcgcctc gtcgcctagt ctataaacga tttatctagc aagaatcatg atgctaaggg
    11881 cgtcaaaaat ccgtatgaaa atagggaaat gacacagtgt tcgatgagca caaggagttt
    11941 catctttttc actaggattt tagcccgggc taaaatcagc tctctgactt cagagggctt
    12001 ttttattgag gttttatcgg taacaatttt ggagactaca aaaacctcaa atggtatcag
    12061 ctaattacac cataaaggtg cgcagttact cggcttttca agccgagtag ctgtatgcaa
    12121 gccccctaga agagcccact ctttacaaag taaagtatag tatgttatac tttacatgga
    12181 agtagtcact gaattccagt tagaaattac tttgtaacta cgttttgagg aggagtaaaa
    12241 tgctttccta cgttcgacat tacccactag cgatagctaa attaatgtgt ctgtgctctc
    12301 ctgaaatctg ctgatttatt actgactaat acaggaggtt tttttatggg acagacaatc
    12361 atatctgcta ttggtgttta tatttccacc agtatcgatt atttaattat tttaattatt
    12421 ttatttgcac agctatcaca gaataaacag aaatggcata tttatgcggg gcaatatcta
    12481 ggcacaggct tacttgtagg ggcgagttta gttgctgctt atgtcgttaa tttcgtgcct
    12541 gaagaatgga tggttggatt gcttggttta atccctatct atttagggat tcgctttgca
    12601 attgttggag aagatgcgga agaagaagag gaagaaatta ttgaaagatt agaacaaagc
    12661 aaggcaaatc aactgttttg gacagttaca ttgctgacaa ttgcgtctgg cggagataat
    12721 ttaggtatct atatacctta ttttgcttcg ttagattggt cacagaccct cgtggccttg
    12781 cttgtgtttg taatcggcat aattatcttt tgcgagatta gtcgggtgtt atcctctatt
    12841 ccgttaatat tcgagacaat tgaaaaatac gagcgaatca ttgtgccctt agtattcatt
    12901 ctacttggac tatacatcat gtatgaaaat ggcacgatag agacttttct gatcgtgtag
    12961 atttttttgt ttcactagga ttttagcccg agctcaaatc agctctctga ttttcagagg
    13021 gctttttcat tgagtataaa tggacaatct taaagttact ctattgtaga gtggcttttg
    13081 atttgattag gagattctta taaatatagt aaaatgaaac aaaaacagga caaatcgatc
    13141 aggacagtca aatcgatttc taaaaatgtt ttaagaagta gaggtgtact attctagttt
    13201 caatttgata tataaatgaa aaccttagct gtttggccaa ggttcatttc ttctatgcaa
    13261 gttcttctct tttcttatct gggttccaga tgaagcttgc gataaagcta aagatgatgg
    13321 ttaggatagc gaggataata gcaaggatga gggcagggat gcggataaac caccagagtg
    13381 gatttggttt tttatcattg tgccattgtt tcgtttcttc gtccagacgt tggaattctg
    13441 tttgaatacg attggcgact gtttcaatgc cttcatcatt cattttcttg atatctgaga
    13501 tatcgattgg atttccaaag ttcatatcga cacgttcacg gctaatcaag cccttcaaag
    13561 tcatgggacc ggtgtaggta accggcatga tacggacctt ggccattttg gcaatcagtg
    13621 ctgcgccccc cttgacatcg tttgagtggc ggctaccact tggaaacatg atgagagagc
    13681 ggtcactttt tttgagaacg ttgataggat atttgatggc tgaggcgctg ggattttcac
    13741 ggtcgatggg aaaggcgcca cacatacgaa tccaccaacc aaagatacgg ttggtaaaga
    13801 gttctttttt tgccataaag atgaactgtt ttggcttggt cgcaaaggcc atataaacag
    13861 gatcccacca ggtacggtga ggcgcaacta aaatataatt ttcatcttga ttaggaattt
    13921 tatcagtatt atgatagtga gcattgccat tgatggacca taagagcaat acaactaatc
    13981 cacgcaaata agtataaaac atgcgatctc cttcgattgt tttcttgtta ttattatacc
    14041 ttatcaaagg agggctggca aacttttccc ttgactagat acatatttag gatgaaatta
    14101 gaattctgtt aaaaaaaatg atataataga atttatggat aaaaataaga ttatgggatt
    14161 aacccaaaga gaagtcaagg aaagacaggc tgagggtttg gtcaatgact ttaccgcatc
    14221 agccagtacc agcacttggc aaatcgttaa acgaaatgtc tttacccttt ttaacgcttt
    14281 gaacttagcc attgctttgg ctcttgcctt tgtgcaggct tggagcaatc tggtcttctt
    14341 tgctgttatc tgctttaacg ctttttctgg gattgtgacc gagctacgag ccaaacacat
    14401 ggtggacaag ctcaatctca tgaccaagga aaaggtcaaa accatccgtg atggtcagga
    14461 agttgctctt aatcctgaag aattagtgct aggagatgtc attcgtttgt ctgcaggaga
    14521 gcagattcct agtgatgcct tggttttgga aggctttgcg gaagtcaatg aagccatgtt
    14581 aacgggagaa agtgatttgg tgcaaaagga agttgacggc ttacttttgt caggaagttt
    14641 cctagccagt gggtcagttt tatctcaagt tcaccatgtc ggtgcagaca actatgctgc
    14701 caaactcatg cttgaggcta agaccgttaa acccatcaac tcccgtatca tgaaatcgct
    14761 ggacaagttg gctggtttta ctgggaagat tatcattccc tttggtctgg ctctcttgct
    14821 ggaagccttg cttttaaaag gcctgcctct caagtcatct gttgtaaact cgtcgacagc
    14881 tcttttggga atgttgccta agggaattgc ccttttgacc attacttcgc tcttgactgc
    14941 agttatcaag ctaggtttga aaaaggtctt ggtgcaggag atgtactctg ttgagacctt
    15001 ggcgcgcgtg gatatgctct gtctggacaa gacgggtacc atcacccaag gaaagatgca
    15061 ggtggaggct gttcttccgt tgacggaaac gtatggtgaa gaggctattg ccagcatctt
    15121 gactagctac atggcccata gtgaggataa gaatccaact gcccaagcca ttcgccagcg
    15181 ttttgtggga gatgttgctt atcctatgat ttccaatctt cccttctcca gcgaccgcaa
    15241 gtggggggct atggagttag aaggcttggg gacagttttc ttaggggcac ctgagatgtt
    15301 gctggattct gaagtcccag aagccaggga ggccttggag agaggatcac gtgtcttggt
    15361 cttagctctc agtcaggaga aattagacca tcacaaacca cagaaaccat ctgatattca
    15421 ggctctagcc ttgctggaaa tcttggaccc cattcgagag ggagcagcag agacgctgga
    15481 ctatctccgt tctcaggagg taggactcaa gattatctct ggtgacaatc cagttacggt
    15541 gtctagcatt gcccagaagg ctggttttgc ggactatcac agctatgtag attgctcaaa
    15601 aatcaccgat gaggaattga tggccatggc ggaggagaca gctattttcg gacgtgtttc
    15661 ccctcatcaa aagaaactca tcatccaaac gttgaaaaaa gcgggtcata caacggctat
    15721 gacaggggac ggggttaatg atatcttggc ccttcgtgag gcggattgtt ctatcgtgat
    15781 ggcggagggg gatccagcaa cccgtcagat tgccaatctg gttctcttga actcagactt
    15841 taatgatgtt cctgagattc tcttcgaggg tcgtcgcgtg gtcaataaca ttgcccacat
    15901 cgccccgatt ttcttgataa agaccatcta ttccttcctg ttagcagtca tctgtattgc
    15961 cagtgcttta ctaggtcggt cagagtggat tttgattttc cccttcattc cgatccagat
    16021 taccatgatt gaccagtttg tggaaggttt cccaccattc gttctgactt ttgagcgaaa
    16081 tatcaaacct gttgagcaga atttcctcag aaaatccatg cttcgtgccc taccaagcgc
    16141 tctcatggtc gtcttcagcg tcctgtttgt gaaaatgttt ggcgcgagtc aaggttggtc
    16201 tgagttagaa atctcaactc tactctatta tctcttgggg tcaattggtt tcttatccgt
    16261 atttagagcc tgcatgccat ttaccctatg gcgtgtcctc ttgattgttt ggtcagtagg
    16321 aggtttccta gccacagctc tcttcccaag aattcaaaaa ctgcttgaaa tttcaacctt
    16381 aacagaacaa acgttgcctg tttatggtgt catgatgttg gtctttaccg tgattttcat
    16441 cctgaccagt cgttaccaag cgaaaaaata aagaaagact gcaatctgtg gattgcagtt
    16501 ttttatacga tggatctatc gtagatctga tgtgcaaggc ctacgtgccg atcatctatc
    16561 ggtgaaccca agagcgaccc tcaagcctgc ttggattgag gtaatagatt caaatatctg
    16621 tagttagact atttgaagtt tgatgtaaga aagagaaagc gacagattga agtaatttta
    16681 actctcttct attgctagaa caaatggtcg gataggttgg tagtttgaaa atgaagatgc
    16741 tatctattgt taaatggaac atagtgttat ttattagaaa atcgtttggt ttatttctta
    16801 tcaaatacga aaagcaactt aaatatttca actaaaatag atgttatgaa gaaaaggtaa
    16861 aatgattttg gcatagtgag gttctgttct atttgatatc atatttttga taaaaacaaa
    16921 aatgtccatt gcaaaggaca aaatgcgaag tatattattt tttgaaagcg atataatgga
    16981 ttcataaagg aggtgtatcg tgtctagaaa acaagaacaa atggaaacgt tgttgctcct
    17041 tttgcgagat agtaaggatt atatatctgc taaagtattg ggagaaaaat taaattgctc
    17101 tgataaaacg gtttatcgcc ttgtcaaggg aatcaacaaa gattgtccgg tagaagcatt
    17161 cattttatct gaaaaaggca gaggtttcaa attaaatcca agaagttccc tcgtggacgt
    17221 tgatgggaat tttacagagg cttttgatcc tgaagtaagg cgtgaaaaat tactagaacg
    17281 tctcttgttg actgctccta agccacattc tatttatgat ttaggagagg aattctacgt
    17341 aagcgagtca gtagtactaa aagatcgtca gatattacaa gagagtctag caatttatgg
    17401 gttagattta aaaatgagac aacgaaagct ttttattgat ggggatgagg ctcaaattcg
    17461 ttcagccatt ctaaatctac tgccaatgtt taatcagttg gatttagagc aaattacaca
    17521 gaataaggtt cagcctcttg acggagaact tgctcacttt tgtttgggat tactgattac
    17581 acttgagaga gaattggggg taaacattcc ctatccatat aatataaata ttttctctca
    17641 cctgtatatt tttatcagta ggaatcgtcg tagtactagt attcatgttg tagcaccttc
    17701 aaaacctact attgttgatg agaaaattta cagtgtctgt caaaaaatta ttcaagaaat
    17761 tgaacaatat tttaggatga aggttgatgc agttgagatt gactatcttt atcaatacgt
    17821 tgtatcttcg agattgcaaa aaccattttc ttccgggaag cttccttttt ctcagcgagt
    17881 tttagatgtc actcattact attttagccg tatgtgtatg gacaatagag agattgaaac
    17941 gacagatcct gactttgttg acttggcgag tcatatcagt cccttactga ggagattaga
    18001 taatagagta cagattaaga atagtctttt atcacaaatt cttttaacct atcctaatct
    18061 ggttaaagag ttaacaacta tttctaaaga agtgagtcta gtatttggtt ttgcttcctt
    18121 gagtctggac gagattggtt ttctagtctt atattttgca cggtttcaag aaaagcgagc
    18181 acgtcctcta aaaacagtag tgatgtgtac atcaggtgtc ggaacttcag agcttttacg
    18241 agcacgatta gaaaagcaat tttctgaatt ggatattatt gatgtagttg cttatcatca
    18301 attagatgag ctgataaatc tatatccaga tttagatttc attgtgacga cggtagcttt
    18361 gcaggaacca gcaagtgtcc cgtttgtcct agttagtgtt tttctaaccg agggtgataa
    18421 acaacgtctt caagcaaaaa ttcaggagat aaactatgaa taatctttcg cttgtcctta
    18481 tggatatatc tgttcaaaat cgtcaagaag cctacaaaga attagcaaat caaatcagcc
    18541 ttcttgtttc tgaagataca gaaaaaatag aagagcttct atattaccgt gagagacagg
    18601 gaagtataga ggttgctaaa ggtgttcttc taccacattg tgaaggaaac tttcaacatc
    18661 atgtcttagt gattactaga ttaaaatcac ctatcagaga atggtcgaag gatatccagt
    18721 gtgttgacct tattatcggt ttggccattg cagtatcaca ggacaagtca tgtattaaaa
    18781 cattgatgag aagactagca gatgaatcat tcataaatca attaaaacag ttaacaaaag
    18841 aagaattacg ggagataata tatggaaatc aaagatattc ttaatgtgag tctgatccag
    18901 acggatttac agatgcagag caaagaagag gtttttgagg cattagctca actattggtt
    18961 gagacgggtt atgtgtctga tagagaccaa tttatcgaag gtctttatca gagagaggca
    19021 gaaggacaga ccggtattgg gaattatatt gctattcccc atagcaagag ttctgctgtg
    19081 gagaaggcgg gggtagtcat agctataaat cacaatgaga ttccttggga gaccattgat
    19141 gggaaagggg tcaaagtaat tgtactcttt gcagttggtg atgatacaga agctgctagg
    19201 gagcatttga agaccttatc actctttgct cgaaaacttg gtaatgacga agttgttgcc
    19261 aaattagttc gggctcagac atctgatgat gtgattgcag ctttttgtta ataagaaaaa
    19321 attttggagg gtatccgtat gaaaattgtt ggtgttgcag cttgtactgt gggaattgcc
    19381 cacacttata ttgcacagga aaaattagag aatgccgcaa aggtagctgg acatgtgatt
    19441 catgttgaga ctcaggggac aataggggta gaaaatgaat tgagtcaaga gcagattgat
    19501 gcagcggatg tagttatttt agcagttgat gttaagattt ctggtatgga acgctttgag
    19561 ggtaaaaaga ttatcaaggt tccaacagaa gtggcagtca aatctcccaa taaactgatt
    19621 gctaaagctg ttgagattgt tacgaaataa ctgaaaatat ttaaggagaa aatatatgtt
    19681 gaaacactta aacttaaaag gtcacttatt gacagccatt tcctatatga ttccaattgt
    19741 ttgtggtgca ggattcttag ttgccattgg tttagcaatg gggggtggtg ttcctgacgc
    19801 tcttgtagca ggaaaattca ctatctggga tgctttagca actatgggtg gtaaagccct
    19861 tggtctcttg ccagttgtta ttgctacagg tttgtcttac tcgattgctg gtaagccagg
    19921 gattgcacca ggttttgttg ttggtctaat tgccaattct gttggttcag ggtttatcgg
    19981 tggtatcttg ggaggttata tagctggttt cttggttcaa gcgattatta aaaaggtcaa
    20041 agtaccaaac tggattaaag gtttaatgcc aaccttgatt attccttttg tagcctcttt
    20101 ggtaagtagt ttgattatga tttatattat tggagcgcct atcgcagcct ttaccaactg
    20161 gttgacgagc ttattacaaa gcttgggaag tgcttcaaat ggtttgatgg gggcagttat
    20221 tggagttctc agtgctgttg actttggtgg cccacttaat aaaacagtct atgcctttgt
    20281 gttgacttta caggctgaag gtgtgaaaga accattgact gctttgcaat tggtgaatac
    20341 tgctacacca gttggatttg gattggccta ttttatcgcg aaattactca aaaaaaatat
    20401 ctatactcaa gaggaaatcg aaacattgaa atcggctgtt cctatgggga ttgtcaatat
    20461 tgttgaaggt gtaattccga ttgttatgaa taacttggtt ccaggtctca ttgcaacagg
    20521 tatcggtggt gctgttggtg gtgctgtttc tttgacaatg ggtgctgatt ctgccgtgcc
    20581 atttggtgga gtgcttatgt taccaaccat gactcgtcca gtagctggta tttgtgcttt
    20641 gttagccaac attgtagtca caggacttgt ctacgcgatt ttgagaaaac caataaaaca
    20701 tacagaacca gttatgactg ttgaagaaga gattgatttg tcagatattg aaattttgta
    20761 agagggtaac gatgtcaaga attgaatttt caccatcttt gatgaccatg gatttggaca
    20821 aattcaaaga gcagattact tttttgaatg ataaagtagc atcttatcat atcgatatta
    20881 tggatggcca ttttgttccc aatattacct tgtctccttg gttcattcaa gaagttcaaa
    20941 aaattagtga cacaccttta tcagttcatc tgatggtcac agacccaacc ttttgggtag
    21001 atcaagttct cgatttacaa tgtgagtata tttgtattca tgctgaagtt ctgaatggtc
    21061 ttgcttttcg tttgattgat aaaattcatg atgcaggtct aaaggctggt gttgtcctta
    21121 atcctgaaac acctgtttct acaatctttc cctacattga tttacttgac aaagtaacta
    21181 ttatgactgt agatccaggt tttgcaggac aacgcttttt ggagtctacc ttgtataaaa
    21241 tccaagaact ccgtcagctt agagttcaga atggttatca ctacatcatt gagatggatg
    21301 gttcttcgag tcgtaagact ttcaaacaaa ttgatgtggc aggaccagat atttatgtta
    21361 taggtcgcag tggattattt ggtttggatg acgatattgc caaagcctgg gatatctgtt
    21421 ctagagatta cgaagaaatg accggaaaaa caatgccaat caaataatgg tttgagaaga
    21481 aatttattag ttaggaggaa tatatgtcac tacaatcagt taacgccatt cgttttcttg
    21541 gcgtagatgc tattaacaaa tctaattctg gtcacccggg aatcgtcatg ggtgctgcgc
    21601 caatggctta tagcctattt acaaagcacc ttagaattac acctgagcag ccaaactgga
    21661 ttaaccgaga tcgctttatc ttgtctgcgg gtcatggatc aatgctactg tatgctctct
    21721 tgcatttaac agggtataag gatgtatcca tggacgagat taaaaatttc cggcaatggg
    21781 gatctaagac acctggtcat cctgaagtga cgcatacgtc tggtgtggat gcgacatctg
    21841 gtccgcttgg tcaggggatt tctactgccg ttggtttcgc ccaagcagag cgttttttag
    21901 ctgctaagta caacaaagat ggttttccta tttttgacca ttatacttat gttatcgctg
    21961 gagacggtga cttcatggaa ggagtgtctg cggaggcggc ttcttatgca ggtcatcaag
    22021 ctttagataa gcttatcgtc ctctacgact ccaacgatat ctgcttggat ggtgagacca
    22081 aagatacttt ctctgaaaat gttcgcgccc gttacgatgc ttatggttgg catacagttc
    22141 tggtagaaga tggaacagat ttagcagcaa tttctacagc aattgagacg gccaagtttt
    22201 ctggtaagcc gagtttgatt gaagtgaaaa cggtaattgg ttacggctca cccaataaaa
    22261 gtggtacaaa tgctgttcat ggtgcaccac taggagcaga agaaacagga gcaactcgta
    22321 agtttttggg atgggattac gatccatttg aagtaccaga ggaagtatat tctgatttca
    22381 agacaaatgt agcggatcgt ggtcaggagg catacgatgc ttgggctagt ttggtgtctg
    22441 attacaaggt tgcttatccc gaagttgcta gtgagattga cgctattgta gctggaaaat
    22501 cccctgtaac cattactgaa aaagacttcc ctgtctatga gaatggcttc tctcaagcaa
    22561 ctcgtaattc gtcccaagat gctattaata cagcagcagc agttttacca accttcttag
    22621 gtggatcggc agacttagct cactccaaca tgacctacat caaggcagat ggcttacaag
    22681 ataaatataa tccattaaac cgcaatattc agtttggggt acgtgaattt gccatgggaa
    22741 caatcctcaa tggaatggct cttcatggtg gtttacgagt ttatggcgga accttctttg
    22801 ttttctctga ctacgtcaaa gctgctattc ggctatcagc cattcaggag ttgcctgtaa
    22861 cttatgtctt tacccatgat tcaattgccg ttggtgaaga tggtccaact catgaaccag
    22921 ttgaacattt ggcaggttta cgctcaatgc caaacttgac tgttatccgt ccagcggatg
    22981 cccgtgaaac tcaagcggct tggcatcatg ccttgaccag taccaccact ccaactgtca
    23041 ttgtcttaac ccgtcaaaac ttggtagttg aagaagggac agactttggt aaggtcgcta
    23101 aaggagccta cgtcgtgtat gataccccgg gatttgatac tattatcatt gctacaggat
    23161 ctgaggtcaa tctagctatc aaagctgcta aggaattggt tttacaaggt ggtaaagtac
    23221 gtgtggtatc tatgccctca accgaactat ttgatgctca agatgctacc tacaaggaag
    23281 acattttacc atctaagact cgtcgtcgtg tggccattga aatggcagcg acccaaagtt
    23341 ggtacaagta tgttggtttg gatggcgcgg tcatcggtat tgacatcttc ggtgcgtctg
    23401 ccccagctca gactgtgatt gataattatg gatttacggt agagaatatc gttgctcaag
    23461 ttaagtccct atagaaacca attacaatga agatacagct gttgtcagac tagcagatgt
    23521 agtgatagac actaatcaga tgattggtta tttaaaaact ataatgagaa tgtaataatt
    23581 tatctacgaa agttataata gatagtatac acaatagagt atactctgaa acggttgcga
    23641 agtacgctaa tcactttgct actgatctag atagtttctt taatcaataa acacagcatc
    23701 cacagattga cttaggatat tgtaagtttt ttgaaagcta gagagaaggt ctctaaaatt
    23761 aaaaaacgca tagcatagga tgttaaaatg aactgacccc aaaagttaga cagaaaaaat
    23821 ctaacttttg gggtgttttt attatgaaac taacttatga tgataaagtt cagatctatg
    23881 aacttagaaa acaaggatat agcttagaga agctttcaaa taaatttggg ataaacaatt
    23941 ctaatcttag gtacatgatt aaattgattg atcgttacgg aatagagttc ggcaaaaaag
    24001 gaaaaaatcg ttactattct cctgatttaa aacaagaaat gattaataaa gtctgacatg
    24061 aaggctggac taaagataga gtttctcttg aatacggtct cccaagtcgt acgatacttc
    24121 ttaactggct agcacaatac aggaaaaacg ggtatactat tgttgagaaa ccaagaggga
    24181 gagtacctaa aatgggacgt aatccaaaaa cgagacctga agagaggaca gaattagaac
    24241 gtcttcaatc agaaaatgag tacctgagag cggagaatgc catcctaaaa aagttaagag
    24301 aactccgatt gaaggaggaa aaagagaaag aagaaagaca gaaattgttc aagaattaat
    24361 gactgagttt tcgttagatc ttcttctaaa atccattaa
//
LOCUS       DAVMAF010000026        24117 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMAF010000026 DAVMAF010000000
VERSION     DAVMAF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.026"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(218..2869)
                     /gene="adhE"
                     /locus_tag="VYO97_001033"
                     /gene_synonym="adhC"
     CDS             complement(218..2869)
                     /gene="adhE"
                     /locus_tag="VYO97_001033"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1753841.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(3169..3579)
                     /locus_tag="VYO97_001034"
     CDS             complement(3169..3579)
                     /locus_tag="VYO97_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753842.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(3581..4009)
                     /locus_tag="VYO97_001035"
     CDS             complement(3581..4009)
                     /locus_tag="VYO97_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1753843.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(4056..4355)
                     /gene="yajC"
                     /locus_tag="VYO97_001036"
     CDS             complement(4056..4355)
                     /gene="yajC"
                     /locus_tag="VYO97_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1753844.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(4472..6448)
                     /gene="tkt"
                     /locus_tag="VYO97_001037"
     CDS             complement(4472..6448)
                     /gene="tkt"
                     /locus_tag="VYO97_001037"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1753845.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            complement(6562..7653)
                     /gene="ulaG"
                     /locus_tag="VYO97_001038"
     CDS             complement(6562..7653)
                     /gene="ulaG"
                     /locus_tag="VYO97_001038"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1753846.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(7765..9438)
                     /locus_tag="VYO97_001039"
     CDS             complement(7765..9438)
                     /locus_tag="VYO97_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1753847.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQFLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKSLLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            complement(9617..10321)
                     /locus_tag="VYO97_001040"
     CDS             complement(9617..10321)
                     /locus_tag="VYO97_001040"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1753848.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVTYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(10323..11186)
                     /locus_tag="VYO97_001041"
     CDS             complement(10323..11186)
                     /locus_tag="VYO97_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1753849.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYVVTEN
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(11190..11855)
                     /locus_tag="VYO97_001042"
     CDS             complement(11190..11855)
                     /locus_tag="VYO97_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1753850.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            complement(11872..12357)
                     /locus_tag="VYO97_001043"
     CDS             complement(11872..12357)
                     /locus_tag="VYO97_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753851.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(12434..12715)
                     /locus_tag="VYO97_001044"
     CDS             complement(12434..12715)
                     /locus_tag="VYO97_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1753852.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(12738..14195)
                     /locus_tag="VYO97_001045"
     CDS             complement(12738..14195)
                     /locus_tag="VYO97_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1753853.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(14326..14949)
                     /locus_tag="VYO97_001046"
     CDS             complement(14326..14949)
                     /locus_tag="VYO97_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1753854.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(15000..15986)
                     /locus_tag="VYO97_001047"
     CDS             complement(15000..15986)
                     /locus_tag="VYO97_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1753855.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(16005..16829)
                     /locus_tag="VYO97_001048"
     CDS             complement(16005..16829)
                     /locus_tag="VYO97_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1753856.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(16804..17175)
                     /gene="rnpA"
                     /locus_tag="VYO97_001049"
     CDS             complement(16804..17175)
                     /gene="rnpA"
                     /locus_tag="VYO97_001049"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1753857.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            complement(17323..18513)
                     /locus_tag="VYO97_001050"
     CDS             complement(17323..18513)
                     /locus_tag="VYO97_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1753858.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSIPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(18564..19517)
                     /locus_tag="VYO97_001051"
     CDS             complement(18564..19517)
                     /locus_tag="VYO97_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1753859.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(19578..20172)
                     /locus_tag="VYO97_001052"
                     /pseudo
     CDS             complement(19578..20172)
                     /locus_tag="VYO97_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(20309..20719)
                     /locus_tag="VYO97_001053"
     CDS             complement(20309..20719)
                     /locus_tag="VYO97_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1753860.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKSEEKPEKKENS"
     gene            complement(20697..21158)
                     /locus_tag="VYO97_001054"
     CDS             complement(20697..21158)
                     /locus_tag="VYO97_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1753861.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            complement(21121..21381)
                     /locus_tag="VYO97_001055"
     CDS             complement(21121..21381)
                     /locus_tag="VYO97_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753862.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(21386..21790)
                     /locus_tag="VYO97_001056"
     CDS             complement(21386..21790)
                     /locus_tag="VYO97_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1753863.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(21783..22049)
                     /locus_tag="VYO97_001057"
     CDS             complement(21783..22049)
                     /locus_tag="VYO97_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEV1753864.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLRKLQADGRITEEQAKAYKEYHDKNGGANRKVND"
     gene            complement(22051..22308)
                     /locus_tag="VYO97_001058"
     CDS             complement(22051..22308)
                     /locus_tag="VYO97_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753865.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVRMYEIEKSESFTILD"
     gene            complement(22540..23496)
                     /locus_tag="VYO97_001059"
     CDS             complement(22540..23496)
                     /locus_tag="VYO97_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1753866.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(23496..23831)
                     /locus_tag="VYO97_001060"
     CDS             complement(23496..23831)
                     /locus_tag="VYO97_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1753867.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRAVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKIRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(23835..>24117)
                     /locus_tag="VYO97_001061"
     CDS             complement(23835..>24117)
                     /locus_tag="VYO97_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753868.1"
                     /translation="EKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMDLHEQSK
                     ADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
BASE COUNT         6846 a         5311 c         4345 g         7615 t
ORIGIN      
        1 caatgtttta gaagcagaag tgtactattc cagtttcaat ctattatata ttttgtttct
       61 attctagttt ttcaaaaagg gcaaaaaggc ctcaatatca taaaaaggca tcctatcagg
      121 tgttcaaaaa ccttgatagg atgcctttta tcatagaaaa attactggat ctttccctca
      181 aattgagttt ttatcttgct tctagactga taaacaatta tttacggcgt cctggtcttt
      241 ctttgtagcc atagtatgca tcttcgatga tttcttgcat atggtctacc attggaagac
      301 gtgggttagc tggtgaacat tggtcttcat aagccaggaa ggctaattca cgagaatgtt
      361 ctttccattc tttttcgtca attccttggt ctctaaaatt catttggatc ccaatacgtt
      421 caccgagttc gtagacagct tttgcgtaag attcaacccc ttcttctgga gtagaagctg
      481 gaagtccaag catgcgtgcg atatcttggt atttttcatc tgcacggtag tagttgtact
      541 taggccatgt tgctgtctta gctggacgtg taccgttgta acggataacg tatggaagca
      601 agatagcatt tgtacgaccg tggattgtgt ggaattgcgc accaatctta tgggccattg
      661 agtgagaaat acctaggaag gcattggcaa aggccatacc agcgattgtt gaagcgttat
      721 gcattttctc acgtgagtgg aagtctgcat tcttaactga gctttcgaga ttttcaaaga
      781 ccaatttaat ggcttgaagt gctaaaccat cagtgtagtc actagccatt tgtgatacgt
      841 atgcttctgt cgcgtgagtc aatacgtcca taccagtatc agcagcaaca aatcctggaa
      901 ctgtcaatac caaagcagga tctacgattg ccacagttgg tgtcaatgag tagtcagcga
      961 ttgggtattt acggttgttt gctttatcag agataacggc aaatggtgtt acttcagatc
     1021 ctgtaccaga tgtagttgga atcgcgatga atttagtctt cttaccaagc aatgggaact
     1081 tgaaggcacg tttacggata tccatgaatt tttggacaag gtcacggaag tccacttctg
     1141 gttgctcgta gaagagccac attactttgg cagcatccat tggagaccca ccaccgagtg
     1201 cgatgatggt atctggtttg aaggcacgca taatctcagt accacggttt acagttgtga
     1261 tatctggatc cggttctaca tccgcaaaga tttggtaaac aaccttattg cgacgaaggt
     1321 ccagttgttc gatgatacga tcaaggaaac caagctctac catggcatgg tcagtaacga
     1381 tcatgacacg ttcaacgtca cgacattttt gaaggtattg aattgaatca cgttcaaagt
     1441 atgtttttga aggaagtttc atccattgca tgttatttct ccgtcttccg acttttttga
     1501 tattcaagag gttaatggca ctaacgttat ccccaactga gttgcgtccg taagaaccac
     1561 atccaagtgt caatgatggc aagaaggcat tgtaaacgtc cccgatacca ccaaaagtag
     1621 aaggtgagtt acagataaca cgaatagctt taacagcttt accaaattct ttagtcaatt
     1681 cttcgtcagc tgtgtggata gctgctgagt gtccaagacc gttaaattca accatttgac
     1741 gagccttagt aataccatct tcacggcttt cagatttcaa aactgcaata actggtgaca
     1801 atttttcacg agtcaatggc tcattttcgc caacttcttt acattctgca gcaagaatgt
     1861 ttgttccttc tggaactgta aatcctgctt gttctgcaat ccaagttgct ggtttaccaa
     1921 cgatgtcagc gttcaatttt gcaccagcac agtttttgct gtttgctttg acgccgaagc
     1981 agaactcttc aagaagagct ttttcttttt tgtttacaaa gtaagtgtgg taagatttga
     2041 actctgctac aaattcatcg taaatttctt tatcaatgat aactgcttgt tcagatgcac
     2101 agaccatacc gttatcaaat gatttagaca tgacgatatc gtgtgctgct tgacgaatgt
     2161 ttgctgattt ttcaacataa gctggaacgt ttccggcacc taccccaaga gctggtttac
     2221 cacatgagta tgcggcttta accatggcat taccacctgt tgcaaggatt gttgcaatac
     2281 cttcgtggtt cataagggca ctagttgctt ccatagatgg ttgagtgatc cactgtacac
     2341 agttttcagg agcaccagct gcgatagctg catcgcggac gatacgagct gcatgagctg
     2401 atgattcttg agctgatgga tggaaggcaa agacgattgg attacgtgtc ttcaatgaaa
     2461 tcaatgattt gaagatcgct gttgatgttg ggtttgttgt tggagtgata ccacaaacaa
     2521 caccaactgg ttcagcaatc agggtcaatc ctgttacatc atcttcttcg ataacgccaa
     2581 ctgtcttagt gtgacgcatg ttgtttacca cgtgttcaca agcaaacaag ttcttagttg
     2641 ctttatcttc aaatacacca cgtcctgttt cttcaaaggc atgtaaagcc aattctccgt
     2701 gggcatccaa agctgctact gatgctttgg caacgatgta gtcaacttgt tcttgatcca
     2761 atttacgcat ttcttcaagg gcaactagag ctttttgaac caactcatct acgtgttttt
     2821 cagcaacgag tttcttttcc tctggtgtca cagttttttt atcagccata ttttcctcca
     2881 tagctttcaa ggaacgatat cctttgttaa ttttttcaca agtttattat aacgcattat
     2941 ttcaactttg taaacagttt cataaacatt tcacaaagaa tttttaaact cttgtgaaac
     3001 ttttctcatt ttcttttctt atcaagtttt atctttgaag tttgtgaaat ttatttcaaa
     3061 tattttttat ttcacaaact tgcttggaag agggatttgg aaagaaaggg atttacaggt
     3121 aagcgcttac cttgtgattc taataatacc tcctttgata aggaggcttt atttttgttg
     3181 aaaaacgcct tgtttaaaag ttccttcata aacgacttct tgttctgttg ttagtttccc
     3241 ttttccttca gcctgaccat ttacaaaatc accttcgtag gtccagcctt ctttagattg
     3301 aaaggtacct tttccgttga aggctccatt gttgaagcca cctgtatatt ggtctccatt
     3361 ttggaaggta atggtacctt ggccattcat tttaccacgg acaagactgc catcataaac
     3421 aatcgttcca ttatcgagtt ttaagacacc ttttcctgga atatttagaa aaaagacgag
     3481 tacagcacag aaaacaatgg taactactgc caaaagctct aaacgtggac gagtcagata
     3541 gacacgatac ttttcgtaaa aattcgtaag attttccacc ttaactctcc ttttctaaac
     3601 gttctaacca agcttgacag ccctcttgaa cacgtcgata ggtttcttca aaatctcctg
     3661 tataccaagg gtctggaaca ctttcagatg aaaatgagta aatcttatct tgacagtcta
     3721 ctggacacat ctgacataag tcagaaatat ttgaagcgtc cattccgata atataatcaa
     3781 aggcttcaaa atcttcctta ctaatctgaa gcgatgtctt gttcttgtca taaggaatct
     3841 catactcttg aaaaattccc tgagtcccct tatgaatcgg attgccatgt tcccaagagg
     3901 aagttgctcg actttggatt tcgtagttat ctgtcattga tttcataaca aactcggcca
     3961 tagggctacg gcaaatattt cccagacaga caaagactaa ttttttcatc ccatttcctt
     4021 tcttttatag aaaaacggga gttcgtgatc ccgtcttatt tttcaattgc gccttctagt
     4081 gaagctgttt ctttcagcgg taatactgtc ttgatagcag ctagttcaaa agtcaagtaa
     4141 actccatcta cgtcaagaac tattgttccc ttctccgtat ctacttcatc gactgttcca
     4201 taaagtccac cgattgtaat cacttcatag cctttttgta gtttatttaa gctttccata
     4261 cgcttttgag cttgtttctt ttgagaacgt tgcataaaga acatcaaggc catcatacct
     4321 acaagcatga ttaaaaaagt aatatttgga ttcattgttt tctcctttgt cttttacata
     4381 ggactactat atcaaatttc agctactttt acaagattgt accttacttt tctggtaaga
     4441 aaaaccagag cttacgccct gatttttagg attatttcaa gtttcgaaca atttttacaa
     4501 gattttctac agtaaagcca tattctgcca atacttttgg tgctggggca gaggctccga
     4561 aggtatcaat acctagaacg gcaccatcga gaccaacata tttgtaccag ttttgacttg
     4621 cacccatttc gactgcaaca cgacggcgga ctgcatttgg aagaatctct tccttgtaag
     4681 ctgcatcttg tttatcaaag acatctgtag atggcatgct gactacgcgg attttttcgc
     4741 cttgactagc caattcttta gcagctgaga cagcaagatt aacctctgaa cctgtcgcaa
     4801 tcaagatggt atcaaagtcg gctgcatttt catatacaac ataagcacct ttagcaacct
     4861 tgtcgaagtc tgttccatct tcaacagtca agttttgacg tgtcaagaca agggcagttg
     4921 gtgttttctc acttgtcact gcaaggtacc aagctgcttg cgtttcacgc gcatctgctg
     4981 gacggaaaac atttagattt ggcatagcac gaagacctgc taaatgctca actggttcgt
     5041 gagtcggacc atcttcccca actgcgattg aatcatgggt aaagacataa gtcacaggaa
     5101 gtccttgtaa ggctgacaag cggacagctg ccttcacata gtcagagaag acgaagaaag
     5161 ttccaccgta tacacgaagt ccaccatgaa gggccatccc gttcaagatt gttcccattg
     5221 caaattcacg aacaccaaac tgaatgttac gattcaagcg attggcatcg tcttgaagtc
     5281 catccgtctt gatataagtc atgtttgagt gagcaaggtc agctgatcca cctagaaagg
     5341 ttggtaattt agctgccaca acattcaagg catcttgact tgaattacga gttgcttgag
     5401 aaaaaccatt ttctaaagct gggaagtctg ctggagtcac ttcgactgga tcacgtccgt
     5461 cgatgatggc ttctacttct gcagccagtt ctggatgagc ttctttataa tctgcaacta
     5521 atttagtcca agcttgataa gctgatgcgc cacggtctgc aacatgttct ttgaaatcag
     5581 catatacttg ttctggaatt tcaaatggtt cgtagtccca accgagggct tgacgagttg
     5641 atgcagtttc atctgctcca agaggggcgc cgtgtacagc attagttcct tgtttgtttg
     5701 gagaaccgta tccaataacc gtcttcactt caatcaaaga tggcttgcct gaagcttttg
     5761 ctgtttcgat agcagcatgg atggcttcca agtctgttcc attttcaacc aaggcagtat
     5821 gccaaccgta ggcattgtaa cggtcacgaa cactttctgt aaaggaatcc tttgtctcac
     5881 catccaagtt gatatcattt gaatcataaa gaacaaccaa cttatcaagt ttttgcaaac
     5941 ctgcgtatga agctgcctcg cttgagacac cttccatcaa gtctccgtct ccacagataa
     6001 cgtaagtata gtggtcaaag atattgtagc cttcacggtt atatttggct gccaagaaac
     6061 gttctgcttg ggcaaaacca gtagcagttg aaatcccttg ccctagagga cctgtcgtag
     6121 catcaatccc tgccgtatga ccaaattctg ggtgacctgg tgtttttgaa ccccattgac
     6181 ggaaactctt aatctcatcc atgctgacat cttcaaaacc agaaaggtga agaagagcat
     6241 aaaggagcat tgaaccatga cctgctgaaa gaataaagcg gtcgcggtta atccagtttg
     6301 gttgagctgg attgatatga agttgttttg taaagaggct gtaagccatc ggagccgctc
     6361 ccataaccac acctggatga cctgagttgg ctttattaat ggcgtcaata cctagaaaac
     6421 gaattgcatt aacagataga tttgacatag aatttccttt ctatttgagg tgattattga
     6481 atcacacatt ctattttact attatatgaa aattatccgt atcatgcaac atacggataa
     6541 cctccaaaga atatttttat attatagcaa agctttaaat tgaatgttag agtcttgttc
     6601 aaaacaatca tcaaaaccac gtggatgatg gtattctact aagtgttgat cttgaggata
     6661 agtgtactta ccgccaactt cccagataaa tggatggaaa tcgtattgca agcgatcttt
     6721 tcgcattttc caaagttcta gaatctcatt agtagaagcc atgaagttag accagatatc
     6781 atagtgaact gggataataa ctttggtacg cagattttct gccatacgaa gaaggtcgat
     6841 agatgtcatt ttgtcttgga tacctaccgg attttcacca tagttattca aagcaacatc
     6901 aattttaaag tctttaccat gttttgcaaa atagtttgag aagtgagaat ctgcaccatg
     6961 atagatggtt ccacctggtg tttcaaagat atagttaaca gccttttgag ccatttcttc
     7021 atctgtaaca gccaatccag caagttcacc gcctgtctca tcagcaccgt tcactgggag
     7081 agttaccaag caagtacggt caaatgattc tactgcatga atcttcatat cttttaattc
     7141 gatagtgtca cctggtttaa caacgatgat acgttctttt ggaacacccc atccttccca
     7201 gattcgtcca cagtggtaag gaccgataaa cttaacatgc tctaacttag gattgttgag
     7261 aattgctgca gctgtgtatg ggtcgatatg atcactgtgg aagtgtgaaa ctaagtaata
     7321 gtctagttcg ttgatagcaa atggatcgat aaccattggc tgaacacgca agtttggttg
     7381 caacttacga acacctgcca tatttgccat ttggtgccca cgaaccatat ctttcacttt
     7441 tttggttgat tttccacggt ttgaccaaag gtccatgaca acgttagcac cagctggtgt
     7501 cttaatccaa gtaccacagt tgcctagcca ccacatggca aaattgcctt caggtacgac
     7561 ttcttcttcg atttcttcgt tcaaccatgt tccccactct gggaaagtgg ctaaaatcca
     7621 tgactctctt gtaatttctt tgacgtttgg catatgttgc ctcctttgga tataattgtt
     7681 atctttgaga ttagtatacc acccttcaaa acactaaaaa agaccaagaa aaacacaggt
     7741 ttgtgttcat cattggactg tccttcaaat atcttccaaa aaagcttgaa ctactatttc
     7801 ttgatttata agtgtttgaa cctcttcttg gaacttacta gccaacttgc tgtctacaat
     7861 ataagacgaa ataagactag aaagattttc actgaaactt ttgctctcat tacgaaatat
     7921 attgtgttta agatagtcta gcattttcaa aatatcttct gcttcaagga taggattaac
     7981 ccttaaaatc ggaaatctgc tatccaaatc atcattagta gtaatcacta catcaacttg
     8041 tgcaatatct tccacactct taaattgttc tgttgtaaat acagtgtcaa tttgctcatt
     8101 tggaaaataa agtttgcatt gtttcagcaa aagtctcgaa accgcaacac cttcatcaca
     8161 aacgagataa acttttttca tatttttttg agacgatggt gtatacttta aaaatcctcc
     8221 aatatgaatc gtcaaatagg caatatcatc gtctgttagc cgaataaacc atgctccttc
     8281 taaaatttcc gcagattttc tagtgactaa aaataattct ccatacttgg atcgaatttg
     8341 ttttgttaga ggatttttag aaaaaatacc gtaagtcttt ctaaataaca aggatttaca
     8401 gtggatcatc aaatttcgta acaaatcatc cttgttctca atctccattc ggatttgtga
     8461 ttcaaaatac cagataaatt cttctaaagc aagttttaat tgccgaaaat catcactttc
     8521 tgcatgaata tccaaatctt tacgatagga gaggaggaga acagctacaa gagaaacttc
     8581 aagtcctgac aaagaaattt caaacttttg aaacaaccgt tctcctaatt tcttagacac
     8641 ctgatactct attctttttc ttatcaaaga aaattctttc tctatatcct gatgtctttc
     8701 ttgatactgt tcaacattat gacagcttaa cagcaaataa ggtagaaact gcaacataaa
     8761 agttatttca tgatggttta ttttcttccc taaatcttgt tcaactaaag gaacctgttc
     8821 cttaaaaaat tggttcattt caacagaaag caaacactca tcatctaacc tctctttcat
     8881 cttatcttct aaaatagata caaaagtggc attttcttcc ataaaaatat gatatagaag
     8941 cgattgaaga tactgaattt tattaagagg gtgggcatga aggtggtatc cctgtgactt
     9001 gctcacttgc aaggtcacct gatattgttc caaagttagt tgataacgaa tgctattcaa
     9061 atcattgaga acagtattcc tagatacctc tgttaactct atcaattttt caatcgtaat
     9121 acgttcttta gaaataccta tccatagtaa cattatcatc atacgttcat gcgcactcat
     9181 gatataatcg tacgaatcta cttccgataa tagtttacaa caagcatctc tctgctcttc
     9241 cgttaagtga ataccaattc gtggaatact aatgatgtga agcgcctcgt cacccagagc
     9301 agcattgatt ttgtcaatgt gataataaat tttccttctt gactctttca aatctttcga
     9361 aatcgccata atcgttttgg acgtttcttg atccatcaaa tattgaagca aatcacaact
     9421 tgccttatcc agtaccatat taaaaaatcc cccaaatcta ttgatatcac agcaattctt
     9481 tttattttac catatatata tcaagcgcta tttctaaaaa atcttttaaa attacttcaa
     9541 ctccaacttt actctcaata aaaatcaaat gtaaaaagag gagctaaatt tatctttttc
     9601 tcctccttca tcgttcttac ttttgaccat aataagcatt tggtccatgt ttacgttggt
     9661 agtgtttttc tagtatgtac tggggagcag gtccaactct tggattgatt tgttctgtaa
     9721 agcgattcat ctttgatact tcctctagta cgacagagtg ataaacagca ttctctggat
     9781 ttttgcccca ggtgaatgga ccgtgattgc gtacaacaat tcctggtact tcaaccgggt
     9841 taagtccgcg atgttcaaat tcttctacga taaccaggcc agtatctttt tcataggtca
     9901 cttctacttc gtccttggtc aaactacggg cgcaagggat tgaaccgtag aaataatctg
     9961 catgggttgt tccgtagaaa ggaatatcac gacctgcctg agcccaacca acagcttctg
    10021 tcgaatgggt gtgaaccaca ctaccaattt ctgaccaagc cttatataat tgcacatgag
    10081 ttgggaggtc ggaagatggt cttaaatccc cttctaggat cttaccatct agatcagtca
    10141 ctaccatgtt ttcaggtgtc aattcgtcat aatccacgcc tgatggtttg ataacaatga
    10201 caccgagttc gcgattgact tcagatacat tcccccaggt aaatttgaca agtccatgtt
    10261 ttggcaatga ttgattggca tcacaaactc gtttacgcat agcattgatt acttgattca
    10321 tcttacatca aacctgcttt cttaatgagt ggatagagaa aagcttgcgc ctcttgaatg
    10381 gctgcgcgtg tttcttctac tgtttcacaa ttttcagacc acatttcgat taggaaaggt
    10441 ccattataat tggtttcctt taaaatatcg aaagcttctt cccatttgac acaaccttgc
    10501 ccgaaaggta catctcggaa ctggcccttt gaattttctg tcactacata agtatccttg
    10561 agatggagag ctgcgatggc atgatgacca agataaaact cactatagat atcattatgc
    10621 catgcagaca cattaccaat atctggatat acaaagagga agggagagtc aatctctttt
    10681 tctatagcca aatatttttc gatgctactg atgaaaggat catccataat ttcaatagca
    10741 agtaccacct gagcttcttc agcccagtca caggcttttc tcaaattttt gataaaacgt
    10801 tggcgtgtct ggggtgactt ttcctcatag taaacatcgt aaccagctaa ttgaatcgta
    10861 cgaactccca agtcttgagc taattcgata cattttttca tgagttctag agatttttcc
    10921 tctagaactg aatcttttga tcccaatggg tagcgacgat ggcctgaaaa acagatagaa
    10981 ggaatacgaa caccagtttc atagatttct ttgacaactt ccaagcgttc ttccttactc
    11041 cagtcaagtc ttgctaaacg ctcgtcacgt tcgtcaatag acatctcgat aaaatcaaag
    11101 cctaactcct tggcaaaatt taaacgttct agccaagtaa agtgtgttgg ggttgccttt
    11161 tcataaattc caattggacg taccatgatt tacccccaaa ttcgtttgat ttcatccttg
    11221 aaggcacgcg ctgctcctgc tggatccaca gcctctgtaa ttccacgacc tgcgataaag
    11281 gtaaagacat caacaccttc aaagagtttg agagtatcta catctagacc acctgttaca
    11341 gatacacgga agcccatgtc aatgagtttt ttaaccttat taaggtcttt ttcaccccaa
    11401 gtttcaccag caagaagagc atcacgagat tggtgataaa tagcttgtga gatacctgca
    11461 tctagccaaa gctgagcttg ttcaaaagtc caatcgccat aaagctcgat ctggatttcg
    11521 cctcgttctc ctcgttcagt cttgatagcc tttagagctg cttccatagt agggatggtt
    11581 gcacaacaga tacaagtcat ccagtctgct ccacgaaccg cattattttt agcaactgtt
    11641 ccaccagcat cagcacattt tgtgtctgcc acaataatct tatctgggaa aaggctacgc
    11701 aagacttcag ccagttcact tccaacttga agcaagcaaa cagttccagc ttcgataata
    11761 tctacttcct gaccaacaga aacagctgct ttaatcgctc cttgcaagtc tgaatggtct
    11821 aatgcaactt gtaaattagg tattcttttt gtcatttctt tattcctctt tctaactttc
    11881 caaatccaat ccttcgagat aagggctatc cttagattct tcaatcatag ccaagacatc
    11941 ttctttagtc tggcaagcct gtaaacgtgc aatagaatct tctaattcaa acagggcaat
    12001 aatttgtgga atggctacac ttgtgtgaat ttttgaactt gttgctgcta gtgccaacaa
    12061 aacagatacc tctttcccat ctgaaaatac aacaggattt tgtaaggtaa ttaatgaaaa
    12121 ggcatcactt tgcacaccag cttcaggtct agcgtggggc atagccatac ctggcatcaa
    12181 gatatagtaa ggcccatact cttcagtcga ttcaatgata gcatcgtaat actctggcaa
    12241 aattgcccca ctttcaatca agggatctac tgctaccttg actgcttctt tccaatcgtt
    12301 agcctctaaa cctagtcgga tcgagtcgtt gtcaattaaa gcttgtttta aattcatatc
    12361 tgcctccttt atttaaagag ggagaaggac agaaacgata actctcagtt tctgtccagc
    12421 cccctccaac cttttactgt agtgcttgac tgagtttttc ggtgatttct ttatcatcca
    12481 tcaagttatc aagcccaatt aacttcccat tagttcgccc ttccaattct tgaatcaaat
    12541 gaagagaagc gattacgatg tcatatccta ctgctaaacc tttagcttca ccgacactgc
    12601 atgaattgac tgtaaaatct gtttgattaa gcttacggag agcattttca accttcatct
    12661 tgataaccat tgatgaaccc attccatttc cgcacgctgc taatacttta accattttat
    12721 ttctcctttt ctagatacta agcttcttct tgaacttcac cgttgtaata tttctcttta
    12781 tcttttgctt tggcaaattg aagttgagga ataacaagca agaagagaca cacaagtaca
    12841 taaccaacaa taccaaggta tttgaagata tatccaaatc caagccatgg gaattcaaag
    12901 tcgatatttc catggtagcc accataagat gccaaatcga gaagggccac acaaagagct
    12961 cctagagcaa cttgaaggac acctgatata aaggaaagga taacagccgc tttccatccg
    13021 ccgcgtttat cagcgtagac cgcaatggct gcattgtcaa agaacactgg tacaaatcct
    13081 gtaataataa gaatcggatt tttaaagacg atgagcaaaa caattgtaat caattgacca
    13141 atcaaaccaa agataaatcc tgacaggaca gcatttggag aaccaaatcc ataagaagct
    13201 gcaacgtcaa ccgctgggaa tgaacctggc aacaatttgt ttgaaatacc ttggaaggcg
    13261 tttgtcaact cagatacgaa cattcggaca ccttgcatca aaacgaacaa gtaaactgag
    13321 aaggtaaagg ctgtttggat aatgtacata aagaaatctt gtttagcagg attgaataga
    13381 gttcctgaag tgatgacttc tttattagac ataatgtctg gacccaagat taaaagaatg
    13441 gctccgaaga atacgagcat caaggtagca gatgcaacaa ctgtatcgtg gaagattgag
    13501 aggaacttag gtaatttaag attgtctaaa ctttcttctt tcttaccaaa gcgtcctgct
    13561 actttatcta caaaccagat tgcaaattgc tgttggtgac caatcgcaaa tccgccacca
    13621 ccagtcaagc gttgagttgc ctcaacagtc atatttgaac taactgccca gtaaagtcca
    13681 cagatgatac caatcgctgc tgtaccgtaa gcattgcgca attgtggtac taagaataga
    13741 accataagag atactgttgc agcttgttgt accatgatgt gaccagtaat aaagagggtt
    13801 cttaccttcg taatctttcg aagagctacg agcaagatat ttattccaaa accaatcaat
    13861 agagctgtag ttgcagttcc aacaaaatct ggaaactctg ctgcaatttt gttgtttgct
    13921 gcagcaagtc caaagtaagg gtcgataacc gctgcaccaa tttggaattt gtagttaaga
    13981 gctgctaaga ttggacgaaa ggttgtaacc aaaccaccag cacccacgtt aagcaacata
    14041 taccctactg ttgctttaac aaaccctgaa aaaacgtcat gggcaggttt tttcaaaagt
    14101 gcatatccta tcaacaccaa taaacctacg aaaaatgcgg gattctgcaa aatattgcta
    14161 gaaaaccaat ttagaacact tgaaacgact tccataattg ttctcctttt gaattgtttt
    14221 tgtaagttta ttatataatg cgaaagcgca aacaaaaaga ccaagaatta cacagcggac
    14281 tgttcactct tggactgtat gatttatgat gttttacaaa ttatcttatt ttctcgtagc
    14341 tagataatag ggaagaattt tagaataaga cttcaataag ccttctacga tttcttcttc
    14401 agacgaattt tctgtcatag gaacatctac cttcactaaa acttttcgaa cttcttgact
    14461 tctcaccttc tcgcgtaaag tacgacgctt ttcttcattc gcctcccacc gttgactttg
    14521 accattagag taggttagat aataaatccc tttaacggtt ggaatgtcta aaactttggt
    14581 ctgcttgccc agtgtttgct catccttctt acgttcgatg aaactgactt ccaaagaaat
    14641 tccaaagtca gtagatgtcc catacaaacg taaggccatc ataggttctg tcacttgtcc
    14701 gtctctctgt agataaaccc aaaaatgtgg tctcaaacgc tgggcctgat tcatccactg
    14761 actagtctgt tggagttgcc attctggatg gcttgcttga aaggctttgg ccagttctgt
    14821 aaaagccttt cgggcctctt gtcctttgtg gcggaacgcc agcatcttct ctcgctctgc
    14881 tcccgcttta tcaggactac ggtactgcaa accagaaaag tctagatagt ctcttatctt
    14941 attcaacatc agtttaacct cctctagcta gcaaaaaatc aggtcaaccc tgattttact
    15001 tattctgtat ctacaacgac atagcgattt ggctcatcac cttcagagta actggtcacg
    15061 ccatccatac gtgaaataat acggtggata atcttgcgtt cactattcga cattggatct
    15121 gtttgatgac tgcgcccttc ttctaaaaca cgaatcgcca atttttgcgc ataggtctgc
    15181 aagacttctg cacggtgttc aacataatca ttgacattaa tagtgatata gaaggttctt
    15241 gaatagcagt tgtaaagata attttgagcc aacagttgca aggccttcaa gactttacca
    15301 tggtagccga taatacgacc tggttcgtta gtatcaattt gcaaattgat gctacgacgg
    15361 ttataatcat ttgaaagtgt agcctcaaca tccatgtcat caataatcgt ttgaacataa
    15421 gcagtcacat ccgtagccac ctgggcaata tcataacttt gctcgacctt caagcccatc
    15481 tctttcaagt cttgattttc agtttgatct tgctcagttt caaggtcatc agcgcctgct
    15541 tcttccctca tcgcttcctc gatttgaagt tcatttaaaa tctcaatgtg accagtttct
    15601 tctaagatag tgctggcatg tctttcatgt tttaagattt cagccttgac ttcatcagaa
    15661 ataccttgac cttcttcctc tattttttta atagcatcaa ccacatgacc aaggtcaacg
    15721 gtttcttcac taaccgtctt cacaggctcg ttcaaatcat tgattttttt cggaacgcct
    15781 tttactacct gttgatttgc tttgacaaca gtcgtttcac taatcgcttc aatatccact
    15841 tgggctggtt ttttaccaaa tagaccaaga aagccttttt tctccctaga aatgactttg
    15901 atatgagcct tcattcttgg aatatctaat tctttcaatc ctttctggat tgcttcttca
    15961 acagttgaac ctgtaaatac taccattacc agattcctcc ttatttattt cgttttctga
    16021 gcctttttct tggcttttct ttttctattt tccaaatctt tttgtgcctg tactacggcc
    16081 tcgcgctctg cgataatctt gaatggatta ttcaagaaat aggtttgcaa gacttgataa
    16141 gcattagaca ctgtccagta tagggcgact ccacctggcg cataaactgc aaagataaaa
    16201 atcaagactg gaatcccata catcatcgca gtcgtagcgc catttcgctc agacaaagct
    16261 ttgttggaca accaagtact taaaaaggtg aatactgctg ctaaaatcgg aagaacaagg
    16321 gttgtatcca cactaccaag gttaatccat aagaaatgac ctgtctttaa aaagtcaact
    16381 cttgataggg cttggaacaa ggccaaaata accggcatct gaatcaaaat cggccaaaga
    16441 gaatctgact gtctgacacc catttcttta aatactttac gcatttcctg ctctagtttg
    16501 gttctgcttt ccatatctcg acctggatat tgttctcgaa gcgccttaat gcgtggctga
    16561 gcttcctgca ttttcctaga agccaccatt tgcacctgaa agactggcaa gaggactgta
    16621 cgaatcaaga ccgtaaagag aataatcccc actccgatac taatatcaaa cgataaaaag
    16681 cgaatgattt tcgcaaagaa gtaaaccaat ttactccaaa aatcagccga ttcggctgta
    16741 atatcgctag ttaccccatt agtcgcacag gctgtcatga ttaacagaga cagtcctagc
    16801 aaactagtca actttagttt ctttttcact cccatttcct tcccggtaaa tctttgataa
    16861 ttttaataca tggagtagat ttttctccat ctctgcgtat cccaaggttt cgactccttt
    16921 tcgagcaatg acaacaaagt cgacatcttc taccagactc ccttttgcat tctggataat
    16981 atgccgaatc cgtcgcttaa tttgatttct agtgacggca ttccccagtt ttttgctaac
    17041 tgatagacct actcgaaaat ggtttttctg gttttctaat tggtagacca caaatttgcg
    17101 attagcaaaa cttgtcccct ccttgaaaat cgccttaaaa tctttctctc tttttacacg
    17161 aaagtttttc ttcaaaacta aactccatct attaaattac tactattata ccatattttt
    17221 caaaaaagcc aatcatagca aggttttgga caatttcatc agaaaaagaa gctggaaaaa
    17281 taactttccc aactccttgt attgaatatt ttttagtttc acttattttt tcaagcgttc
    17341 aacgtcacgg gcaatgacta attcttcatc tgttggaata accaagacac ggattttagc
    17401 tgcctctgtt gagatgtctc ctgtaacgcc aaagacattc ttttcatcat caacatcaca
    17461 accaaaccac gaaatccctg agattacatc acgacggaaa ctctctgcat tttcaccgac
    17521 acctgctgtg aaaacaatgg catctgctcc atttagcact gcaaggtact gaccgatatg
    17581 tttttggata cggtcaacat acatttcata agccaagcta gcctcgtgat tcccttctgc
    17641 tacagctgct tctatatcgc gcatatcgct agaattagca gaaactccca aaagacctga
    17701 ttcacggtta agaacacgac tgatatcttc tggtgtgtta aaatcctctg tatattgcat
    17761 taaataagga atgatagctg gatcaatatc ccctgtacgc gttcccatca taataccacc
    17821 aagaggagtg aaccccatag aagtgtctac agatttgccg gctttcacag ctgtaattga
    17881 gcctccgtta ccaatatgac aggtaattaa cttcaagtct tctaatggac gtcccaagag
    17941 ttttgcagct tctcctgcta caaactggtg acttgtacca tgagcaccgt atttacgaac
    18001 cttgttttct gtgtaatatt ttgttggtag agggtagcga taagctttct ctggtatact
    18061 tgtgtggaag gaagtatcaa aaacaactac actggtaatg tctggcaaca attccttgaa
    18121 ggcacgaaca cctgctgcat tggccgggtt gtgtagagga gccaacaaac tcaactcttc
    18181 aactttttct aaaacatctc cctcaacaac tgttgattct ttgaaatatt ctccaccagc
    18241 aacaacacga tgtccaacac ctgtaatctc gtcataagcc ttgataatat cgaaacgaat
    18301 caagtcatcc aataaaattt taacggcttg tatatgattt tcaatatcca aaatttgttg
    18361 ttcagaacgg ccgtcaaatt ttacagttga aattgaatct ttcaaaccga tacgttcaat
    18421 caaacctttc gccaatactt tttcttctgg cattaagtat aattgccatt tcaaacttga
    18481 acttcctgca ttgattgcaa ttgtttttgt cataacatga tacccctttt taagcgtttt
    18541 caactattat aacaaaatct attttatatt tcagtacctt gagtccattt ttgaaaattt
    18601 tctttaaatt tcattaaaac acttgcatct tgcaagctag caagtggata aacaaaaggc
    18661 tccactgcta tttcactttt cttctgtagg ataaaaatag tcttagattg tttagcatta
    18721 gcaaagagat tttcaggtaa actaatcata gcaaccagac ttgcctcctc tttcaaccag
    18781 actttcaaca aatcactttg aggactggtc aacaaatcac tcggagctag aaaaatagca
    18841 tatccatctg acttaaggta cttaaaccct tgttccatga gcaagtgatg agcgtaagta
    18901 tgttcttggc tagaagcaac ttgatggcgc gacgcaacgg catcatcagg ataatagcca
    18961 acaggcaagt cactgatgac cacatcgctt tctttgagca tttgtgggcg aacggcatct
    19021 ccttggacaa agccagcctg caaaccaatt acatctgcca tgctagctgc cagatcaatc
    19081 agcaaatcat ccacttccat tcccaagtaa tccacctttt tatcaagcga ggtcaagaaa
    19141 gtagcgccta gaattcccat cccagaaccc atttcgagga tagtaatttc ctcctctgta
    19201 aacaactctt ccacaataaa taccaaaagt aaggcaatag catctggcgt aaactggtga
    19261 ttggcctgca agggttctgt ttgcccagcc ttcatcaaga gaaactggta ggtcttgagc
    19321 cattcttctt tgcgtagtgc taaacgctta agggcctgat tgttgtcttt gacctggttt
    19381 agctcagttt caccatccag atagatgcta ttttgctcca ccaaggcgtc ataaaagttg
    19441 gtcgccaaat cactttggat gacttggaca ttctctagta aatagatata agcttgttca
    19501 attttttcaa aatccatatt cctatcttat caaatttccc ttcttttttc catccttata
    19561 gaaaaatcac tccctgacta ggcgagtgat ttttgggata ctgttaaaaa gaattctgag
    19621 tgattccctt gaaacaggtc ttcagcgcta tagagaatct gactatgcac agatgaaaaa
    19681 ccatgctcaa ttagcttgtt ttccagttca gctaaatcaa atccatgatg attagcttct
    19741 gtcttggtaa aatcagcaat gatgagtttc ccatcttcct tcaaatgttg atgaaacagt
    19801 gagagagccg catccaaatc aggcatatga tgaagaaccc gactaacagc aaggcaatca
    19861 aactctttct ccaagggatt tttcggtaaa tcttgctcca aaaactggat attcttgatt
    19921 gcttgctgct ccactttcaa acgagcttgc tccaacattt tctcagaaat gtctacaaga
    19981 gtgactgact tagcctgctt ggctaggggt taggggcaag gctaatagac ccgtgccacc
    20041 accgaaatct aaaatttctt tgtctgatag aagatcaatc tgtttctcgg ctgcttgaca
    20101 taccaagttt gcgaggaaga tatttttagg ggaatcgaaa gtttctgctt tgtggttaaa
    20161 atcatgtttc atatctttaa tttagcacaa agtatagtaa actgaaataa gatataaaca
    20221 aatgaattgg agcttaacat ccatttccag caattttcta gaaactacag tggactattc
    20281 tggattcaac gcattatagt tgaattgact atgaattctc tttcttttca ggcttctctt
    20341 ctgatttttt ctccgctttt tcgcttgaat cagtcgctac ctcttccttt ttatctcttt
    20401 tctcttcttt gattttgact gaaggaaaca gaaactcata ttggctctta gacgtacgaa
    20461 cagtcgtcac caagcttgtt ttcttgtttt gatagcttac ctgacctaga ttaaagacct
    20521 gttccccact ttcttgctcg gctttatcct tggttcgttt agccattgca aaagcaacca
    20581 atttttcttt attcaaagca tagtcttgat agtgggcgac ttgtcggttc aagtaaaatt
    20641 gcaacaaaag actaaagatg gctgctatgg tgactgcgta gaggagaaca cctgccttaa
    20701 cttttttctt tttccacacg atagatgaac tccctttcta agcctttttg aaactggaaa
    20761 cgaaaatgaa ccagttgatt gtcctctgta atctgagctg atttgaggcc ataaaccata
    20821 ggctgatacc cccgtccact agcatcggtt ttacgaaaat cgtctgactt tgacttaccg
    20881 atggcgatgt ccttgccatc ttgcttcatg tataggcgat tgccttctac tttttcgaac
    20941 tgcgaacggt ctaattctgc ctcaagttgg tccacaaaca agagccactc cttttgctcg
    21001 ctttgttgct ggtagcgaac ttctgaaatg aggagctgac tcatagtttg aaaaaggagt
    21061 aatcccccac tgatgacaat gagggcaagt agggattcta acaaggtaaa agccttgacc
    21121 ttatggctct ttgattgcca acaactgttc tgaaccatgg tagacctcca atcctttttc
    21181 actagaaaat acctgaatct caactccgtt gatgcttacc tgattttgcc ccgtctgcag
    21241 ggccatctta gctaccctca agacttcttc cttttgcaag atttttgctt cctcttgcct
    21301 atttttttga atttgtccca aaaggagggt cgcaatgctg gcaaagatag ctagagcgac
    21361 tactgcttcc agtaaaatca ctgccctaat tttttgtttc cttaatgcgt ttaatttttc
    21421 catttcctag atataattga tagcgaatcg ctcctttact ggtctgaaat tcaaccttag
    21481 ccagggacga attgccccca gctcggtcaa atgtaatact ttggcctgat ggtgcctgaa
    21541 ttcctttagg aactgtcaac ttttgactgc cattgctaag cgtctgccca tctaaattta
    21601 gattagtctt ttgctgactg gctacactgc gtttttgggt ttcccgatag agttcttcaa
    21661 actccataaa gaaaatctgt tcctctaccg ccgcaaaagt ggactgaaca gagccggaca
    21721 agcccaaggc aaggatactc acaagaccca aaaccaagag actttccagc atggtaaagg
    21781 ccttaatcat tgactttacg atttgctcct ccatttttat catggtattc tttataagct
    21841 ttagcctgtt cttccgtgat gcgtccatct gcttgtaact ttcttaggct agcatcttca
    21901 ttcttttcta agctataaag ttctgcctgg ctttccacca ccttaacaac agctgctttt
    21961 cctttgtcat tgactgcttc tttttgcttg gtcagattag gtacaaagag caagaaaagc
    22021 acgctgataa tcagcaagac caccaacatt tcaatcaagg atagtaaaac tctcactctt
    22081 ttctatctca tacatcctta ccgaatgcaa cacgagatca cgatacttcc aagtgctgac
    22141 cagatactca ataacctctt ggtcctctat cctgcattcc ataagtaata atagtttgac
    22201 cgtgtattca ttcttcaaaa tcggaacctg ataagtcaca tctacccaat gctcaaaacc
    22261 taagtctgtc tgctctacat ttgctagttc aatatttaaa atcttcattt ttattcctcc
    22321 tacttatcta ttcgtaaaag aagataaaaa gttatgaaaa aatcattact tttttaattc
    22381 agacagtact ttcagagcaa ttttgttaat gtcaacaaaa ttggcaacca agcgatttaa
    22441 tactatttgt tgacgccaac aagtcaattt tcagacaaac aaaaaaaccg caagcctgag
    22501 cctgcggtga aagaacaatt tagaaagttt cctttctatt tatttaactg taatcaagcc
    22561 atctggctct actgtgaact ctggcttatc tgccagtgtt ccgtctggtt tgaggtagta
    22621 ccagcctgtt ccgtctgcgg actggacgaa ggcgtttgat accatagcgc cttctttagc
    22681 gtctaagtag taccaagtgt tcttgtactt gacccagcct gtcttcatgg caccttctac
    22741 atcaaaatag taccacttct cagcgatttt cttccagcct gtggccattt cgcctgattg
    22801 gtcaaagtag taccaattac cgtctgtgtg cttcttccag cggtctgaaa gcatatagcc
    22861 tgagccatcg aaataatacc aggtaccgtt gattttctca aacttatctt ttggataaga
    22921 gccgtctgag tgtacgtacc agtagccagt gccatttttc tgccagcctg ttgcagctct
    22981 caagccgttt tcgatgtctt gcttaaactg ttcacggcta atgccccatt tagcaagata
    23041 tggatatgga tccacatggt ctgagtggtt gtttggttgg ttattggtac agtattcatg
    23101 cgtcttgata cctgccaagt cgtctgtatc aagagtcttc ggcaagcctg cttcgtccgc
    23161 tagattgcgt aagagttcga tataaaggcg gtagtccgtc atgaactctt ctttagttga
    23221 atggctttca atcagttcaa ccgctgcgta actctcagta ttccaaccgc ccccaacatc
    23281 ccaacttccg ttgttcacag gacctacttg catgacacgg ccatttccga caacatgtga
    23341 aaagaaccct agttcagggt cctttctata gtggtaatca gcctcatttt gagctgttga
    23401 gttacggttg cctgttgagt gggcgtgtac ttgtcgataa ggctgcaccc caacctgggg
    23461 caagcctgtt cgtagtctgt ttctatcgat atccattact cttgtccttt ccaagcgtcg
    23521 ttcatctgtt tcactgctga ctctacgaag gtgtctaagt ctttgtcagt catgctaata
    23581 ttgtattttg taagctcagc acgaatttta gcgcgagcct gtgccagttt ttcatctccc
    23641 ttgtagccag tttcagcagc tacctgctcc acggcattaa ctgcgttctt ggccaagatt
    23701 tcaacaatct tgatagtctt ttctccacct tttttgacga ggtattcctt gactgcccta
    23761 actacaaccc ctgccaaaat gactaggatg ctgattgctc cattagtaat gatttcagta
    23821 atttgttgca tttgttattc tcctttttcg atttcttcca tgcggtcgtt catgcgaacc
    23881 atttcttttt gaatgtctcc gaccgtgtga gtgattgtgg ttaattctgt agtggtcttt
    23941 tctaggtgag tcatcaaacg ctcttctcgt ctattagagt cggcctttga ttgctcgtgc
    24001 aaatccataa tcttcttctc tcgcttgtcc gaagtcttga taagatatcg aatgataata
    24061 aagaaaagca agataaacaa aatcgcccaa gctacctgac tttgagcgat tttttca
//
LOCUS       DAVMAF010000027        21162 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMAF010000027 DAVMAF010000000
VERSION     DAVMAF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.027"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(60..1397)
                     /locus_tag="VYO97_001062"
     CDS             complement(60..1397)
                     /locus_tag="VYO97_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1753869.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1407..1673)
                     /locus_tag="VYO97_001063"
     CDS             complement(1407..1673)
                     /locus_tag="VYO97_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1753870.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1938..2324)
                     /gene="rplQ"
                     /locus_tag="VYO97_001064"
     CDS             complement(1938..2324)
                     /gene="rplQ"
                     /locus_tag="VYO97_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1753871.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2336..3271)
                     /locus_tag="VYO97_001065"
     CDS             complement(2336..3271)
                     /locus_tag="VYO97_001065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1753872.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3314..3697)
                     /gene="rpsK"
                     /locus_tag="VYO97_001066"
     CDS             complement(3314..3697)
                     /gene="rpsK"
                     /locus_tag="VYO97_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1753873.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3715..4080)
                     /gene="rpsM"
                     /locus_tag="VYO97_001067"
     CDS             complement(3715..4080)
                     /gene="rpsM"
                     /locus_tag="VYO97_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1753874.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4098..4214)
                     /gene="rpmJ"
                     /locus_tag="VYO97_001068"
     CDS             complement(4098..4214)
                     /gene="rpmJ"
                     /locus_tag="VYO97_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1753875.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4239..4457)
                     /gene="infA"
                     /locus_tag="VYO97_001069"
     CDS             complement(4239..4457)
                     /gene="infA"
                     /locus_tag="VYO97_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1753876.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4574..5212)
                     /locus_tag="VYO97_001070"
     CDS             complement(4574..5212)
                     /locus_tag="VYO97_001070"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1753877.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5363..6673)
                     /gene="secY"
                     /locus_tag="VYO97_001071"
     CDS             complement(5363..6673)
                     /gene="secY"
                     /locus_tag="VYO97_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1753878.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6686..7126)
                     /gene="rplO"
                     /locus_tag="VYO97_001072"
     CDS             complement(6686..7126)
                     /gene="rplO"
                     /locus_tag="VYO97_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1753879.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7271..7495)
                     /gene="rpmD"
                     /locus_tag="VYO97_001073"
     CDS             complement(7271..7495)
                     /gene="rpmD"
                     /locus_tag="VYO97_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1753880.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7467..7961)
                     /gene="rpsE"
                     /locus_tag="VYO97_001074"
     CDS             complement(7467..7961)
                     /gene="rpsE"
                     /locus_tag="VYO97_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1753881.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7979..8335)
                     /gene="rplR"
                     /locus_tag="VYO97_001075"
     CDS             complement(7979..8335)
                     /gene="rplR"
                     /locus_tag="VYO97_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1753882.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8419..8955)
                     /gene="rplF"
                     /locus_tag="VYO97_001076"
     CDS             complement(8419..8955)
                     /gene="rplF"
                     /locus_tag="VYO97_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1753883.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9147..9545)
                     /gene="rpsH"
                     /locus_tag="VYO97_001077"
     CDS             complement(9147..9545)
                     /gene="rpsH"
                     /locus_tag="VYO97_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1753884.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9830..10099)
                     /gene="rpsN"
                     /locus_tag="VYO97_001078"
     CDS             complement(9830..10099)
                     /gene="rpsN"
                     /locus_tag="VYO97_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1753885.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10117..10659)
                     /gene="rplE"
                     /locus_tag="VYO97_001079"
     CDS             complement(10117..10659)
                     /gene="rplE"
                     /locus_tag="VYO97_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1753886.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10683..10988)
                     /gene="rplX"
                     /locus_tag="VYO97_001080"
     CDS             complement(10683..10988)
                     /gene="rplX"
                     /locus_tag="VYO97_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1753887.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11066..11434)
                     /gene="rplN"
                     /locus_tag="VYO97_001081"
     CDS             complement(11066..11434)
                     /gene="rplN"
                     /locus_tag="VYO97_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1753888.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11460..11720)
                     /gene="rpsQ"
                     /locus_tag="VYO97_001082"
     CDS             complement(11460..11720)
                     /gene="rpsQ"
                     /locus_tag="VYO97_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1753889.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11745..11951)
                     /locus_tag="VYO97_001083"
     CDS             complement(11745..11951)
                     /locus_tag="VYO97_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1753890.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11961..12374)
                     /gene="rplP"
                     /locus_tag="VYO97_001084"
     CDS             complement(11961..12374)
                     /gene="rplP"
                     /locus_tag="VYO97_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1753891.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12378..13031)
                     /gene="rpsC"
                     /locus_tag="VYO97_001085"
     CDS             complement(12378..13031)
                     /gene="rpsC"
                     /locus_tag="VYO97_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1753892.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(13044..13388)
                     /gene="rplV"
                     /locus_tag="VYO97_001086"
     CDS             complement(13044..13388)
                     /gene="rplV"
                     /locus_tag="VYO97_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1753893.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13400..13681)
                     /gene="rpsS"
                     /locus_tag="VYO97_001087"
     CDS             complement(13400..13681)
                     /gene="rpsS"
                     /locus_tag="VYO97_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1753894.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13785..14618)
                     /gene="rplB"
                     /locus_tag="VYO97_001088"
     CDS             complement(13785..14618)
                     /gene="rplB"
                     /locus_tag="VYO97_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1753895.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14636..14932)
                     /locus_tag="VYO97_001089"
     CDS             complement(14636..14932)
                     /locus_tag="VYO97_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1753896.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14932..15555)
                     /gene="rplD"
                     /locus_tag="VYO97_001090"
     CDS             complement(14932..15555)
                     /gene="rplD"
                     /locus_tag="VYO97_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1753897.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15580..16206)
                     /gene="rplC"
                     /locus_tag="VYO97_001091"
     CDS             complement(15580..16206)
                     /gene="rplC"
                     /locus_tag="VYO97_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1753898.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16423..16731)
                     /gene="rpsJ"
                     /locus_tag="VYO97_001092"
     CDS             complement(16423..16731)
                     /gene="rpsJ"
                     /locus_tag="VYO97_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1753899.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16997..17614)
                     /locus_tag="VYO97_001093"
     CDS             complement(16997..17614)
                     /locus_tag="VYO97_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1753900.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17611..18201)
                     /gene="nrdG"
                     /locus_tag="VYO97_001094"
     CDS             complement(17611..18201)
                     /gene="nrdG"
                     /locus_tag="VYO97_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1753901.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18194..18700)
                     /locus_tag="VYO97_001095"
     CDS             complement(18194..18700)
                     /locus_tag="VYO97_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753902.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(18744..18884)
                     /locus_tag="VYO97_001096"
     CDS             complement(18744..18884)
                     /locus_tag="VYO97_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753903.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18896..21103)
                     /gene="nrdD"
                     /locus_tag="VYO97_001097"
     CDS             complement(18896..21103)
                     /gene="nrdD"
                     /locus_tag="VYO97_001097"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1753904.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALRKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
BASE COUNT         5824 a         4738 c         3987 g         6613 t
ORIGIN      
        1 ttaatgatta tagtatacac atctaatctt aaatagaact taaaatttct cctattttct
       61 taatttttaa aactatgaat tggtgctggg atttgtccac cgcgagagat gaagtcgaca
      121 gacgaagccc cattaaccct cataacgggt gcagttccta atagaccacc aaactcaatc
      181 atatcgcctt cttttccttt gggaatgata cgaacagctg ttgttttcat gttgataaca
      241 ccgattgctg cttcatccgc aatcatagcc gcaatagttt cagcaggcgt atcttctggg
      301 atggcaatca tatccaatcc aacagaacag atagccgtca tagcttctag tttttctaaa
      361 ttaagagagc cattttgcac tgcagcaatc attccttcat cctcagaaac agggataaag
      421 gcaccagata aaccaccgac ctggttacag gccatcactc cacccttttt aacttggtcg
      481 ttcaagaggg ccaaggcagc cgtcgttcca tgcgtgccaa ctgtttctag ccccatttcc
      541 tcaaggacac gtgccacaga gtctccaacc gcaggggttg gtgccaaact caagtccaca
      601 ataccaaact ccacacccag tctctcactg gccatttgac caaccaattg accgatacga
      661 gtgattttaa aggcagtttt cttaactgtt tcggctacta catcaaagct ctgtccacga
      721 actttttcca aagcacgttt cacaacacca ggaccagaaa ctccgacatt gatgataaca
      781 tctgcttccc caacaccatg aaaggcaccc gccataaatg gattgtcctc aacagcatta
      841 gcgaatacaa ccaacttggc cactcccata tctgaaagat ttgctgtttc cttgataatt
      901 cgtcccatat ctgccacagc cgtcatatta ataccagact tggttgagcc gatattgact
      961 gacgagcaga ccttatccgt ctcagccaaa gcgcgaggaa tggaattgat gagaatctca
     1021 tctccctttt gataaccttt ttgtactaag gcagaaaaac caccaataaa gtccacacca
     1081 atctctttcg cagccttatc aagcgctttt gccagaacca cgtagtccgt cgcatctgtc
     1141 gctgccccaa tcagagaaat aggtgtcacc gatacacgct tattaacgat aggaattccc
     1201 aactcagccg caatttcatc accaacagct actaaattag ccgcctttgt cgtaattttt
     1261 tgatagattt tctccgcagc acgattgata tctggatcga tacagtccaa aagagaaatc
     1321 cccatggtaa tggttctgat atcgaaattt tgctcctcaa tcatggcgat ggtttcagta
     1381 acttgtctaa tatccatgat aacctcctag atattataca tagcttcgaa aatcgctgca
     1441 ctctgaatat tgatttttac attcaaagtt tgcccaaaag cttcaaattc attacgaaga
     1501 taggtaaaat cttgcttttc atcactagat acaatagcca tcatcgtaaa atattcatcc
     1561 aagacagttt gagagatatc gtcaatattc aatcctaatt ctgcaatttt accagaaaca
     1621 cctgcaacaa ttccagattt atctttacca acaacagtta taatcgcttt cataagcaaa
     1681 ctccaattct tcttaaaata agaaacctta acattaaaca ggattctttt caaatagtat
     1741 agcataattc taacgaaaat actcaaaaac gcctgcttac gaagttgttc ttaagaaaaa
     1801 aacaatttcg taaacaagcg tctactatcc caacttatga tgagtgttcc cgctaggacc
     1861 gaaatcctag cggtagacca gagctagact aagagcacaa gactccatca tcataacact
     1921 caacaaaatt gatgatttta tactaattcg atgatcgcca ttggcgctgc atcaccacga
     1981 cgtggttcag ttttaaggat acgagtgtat ccaccgttac gttcagcata acgaggtgcg
     2041 atttctgaga acaatttttg aagtgctgta gtagaagtgt acttatcagt tgcttcatca
     2101 tagttttcag atgcgatttc attacgtacg aaagcagctg cttgacgacg tgcatgcaaa
     2161 tcaccacgtt tacctagagt aatcattttt tcaacagttt tacggatttc tttagcacga
     2221 gcttcagttg tcacgattga ttcgttgatc aaaaggtcag ttgtcaaatc gcgaagcatt
     2281 gctttacgtt gtgagctagt gcgtcctagt ttacggtaag ccatgtattc ctcctttatt
     2341 tatcttttaa tccaagaccc aaatcaatga gtttgagttt cacttcttcc aaactcttgc
     2401 gtccaagatt tcgtactttc atcatctctg cttcagattt ttctgtcaaa tcatgcacag
     2461 tattgatacc ggcacgtttt aaacagttgt atgaacgcac agacaagtcc agttcctcaa
     2521 tcgtacgatc taaaatacgg tcgtcagatt cagtatcagc ttctttcatc acttcagttg
     2581 acttagcaat ctcagtaaga tttgtaaaca aatcaagatg ttctgtcaaa atacgtgctg
     2641 aaagccctaa agcatcttct ggaataattg ttccatttgt caagatttca agggttaatt
     2701 tgtcgaaacc atcattgcta cctacacgag caggttccac ttgatagttg acttttgtaa
     2761 ctggtgtata aatagaatct acagcaagtg ttccaactgg tgcattatcc tttttatttt
     2821 catcagcagg tacatatcca cgaccactgt taacagtcat agtcgctttt agagaagaac
     2881 cttcaccgat tgtaaagaga taatgatctg gatttacaat ttcaatatcg ctatctgtca
     2941 aaatgtcacc agctgttact tcagcaggac cttcaacatc cagttcgatg attttttcgt
     3001 cttcaacgta cgatttcact gcaattcctt taatgttcag aatgatttgc atcacgtctt
     3061 cacgaacacc tggaactgtg tcaaactcat gtaacacacc atcaatgttg atagatgtca
     3121 cagctgctcc tggtagagaa gctagaagta cacgacgaag agagttacca agagttgtac
     3181 cgtagccacg ttcaagtggt tcgattacaa acttgccata atctttattt tcatcaattt
     3241 ttgttatatt tggtttttca aactcgatca tttagttact ccctcttaaa cgaaaagcag
     3301 tgtaatgcga tgattataca cggcgacgtt ttggaggacg agcaccattg tgtggcactg
     3361 gagtcacatc acgaattgct gttacttcaa gaccagcggc agcaagcgca cgaatagctg
     3421 actcacgacc agaacctgga ccttttacag taacttcaac tgatttaaga ccgtgttctt
     3481 gtgcagattt agcagcagct tcagaagcca tttgagcagc gaatggtgta gatttacgag
     3541 aacctttgaa accaagagca ccagctgatg accaagcaat tgcattacca tgcacatcag
     3601 taatcataac aatagtgtta ttaaatgtag cgtgaatatg agcaatacca gattcgatat
     3661 tctttttcac acgacgttta cgtgttggtt tagccaagac ttttacctcc tatattattt
     3721 tttcttacca gcaatcgcaa cagctttacc tttacgagtg cgagcgttgt ttttagtgtt
     3781 ttgtccacgg acaggaagtc cacgacggtg acggatacca cggtatgaac cgatttccat
     3841 caaacgtttg atgttcaagt ttacttcacg acgaaggtca ccttcaactt tgattgcatc
     3901 cacttcacga cggatagcat cttcttgatc tgatgtaaga tcacgtacac gaacatcttc
     3961 tgagattcca gcagcagcca aaattttctt agatgttgca agtccgatac cataaacata
     4021 agtcaatgag attactacgc gtttgtcatt tggaatatca actccagcaa tacgagccat
     4081 gttttctcct ttctatctta tccttgacgt tgtttgtgtt ttggatttgc tgggcaaatt
     4141 accataacac gaccattacg acgaataact ttacagtatt cgcaaattgg tttgaccgat
     4201 ggtcttactt tcatttctta tccctccaag tttttcgatt atttaaagcg gtaagtgata
     4261 cgtccacgtg tcaagtcata tggactcatt tcgacagtaa cacgatctcc cgctaaaata
     4321 cgaatatagt ttttacgaat tttaccagaa actgttgcta aaatctgatg tccattttca
     4381 agttcaaccg taaacattgc attcggcatt gtatcaacta ctttgccttc aacttcaatc
     4441 acatcgtctt ttgccacgca aaagcacctc cataaatttc gattcgatgc ctctaaacac
     4501 agagacaaca attataagtc agactatctc agtataacat ttgtagcgga ttttggcaag
     4561 tgtgaaaaac gctttatttc aaatttgtca atactttttc aatatctgag aagacatcat
     4621 tgatatcttg attaccttcg atgtcatgaa ccaaaccttt ggcacggtag tgagcaatga
     4681 ttggttctcc ttgagcaata ttaacatcca aacgacgttt tactgtctca ggcttatcat
     4741 cttcacgttg gtagtaatct tcttctttat agtcaactgg tgggttaaag accttgtgga
     4801 aagtttctcc agttacgcgg tggatgatac ggccactcaa acgttccaaa aggctgttag
     4861 ggttcacttc aatattgata acaccttcta gttcaatgcc aagttcagcc aatgttttgt
     4921 ccaaggcctg agcttgttca attgtacgtg ggtaaccatc caataagaat cctgtttctt
     4981 taatatcatc ttgtgaaaga cgttctttta cgattccatt tgtaacttcg tcaggaacca
     5041 attcaccctt gtcaatatat gacttagcaa gaacacccat ttcagtttga tttgccattg
     5101 cagcgcggaa catatcacct gttgagatat gtgcaacatg gaattgttct acgatttttg
     5161 ctgcttgagt tcccttacct gcaccaggta agcccataat caaaagattc atgatttgat
     5221 ctccttattt tatttttaaa tagaagcaaa aagtcttttc acccctattt aaaaacaaaa
     5281 tagaagaggg gagatccaac tcttacaaat gtcagagttt aaacctccac tccctcagta
     5341 tttactgatt cagtaaatac ttttattctg ttctgtccat gaaaccaaca tacttacgtt
     5401 tcaataggta accttccaat tgcttgattc cttcgatacc tgtagagata atgatcaaga
     5461 gacttgttcc accaaaggca acaacatcag aaagaccaaa tacatcttta gctgcaatcg
     5521 gtaaaatgga aatcacacca aggaagaggg aaccaacagt tgcaagacga cgaagaagtt
     5581 tagacatata ttcttctgta cctttaccag gacgaactcc atggatatag gcaccactct
     5641 tttgtaggct ctctgctgct ttttcaggat taatctgtac aaacgtatag aagaatgtaa
     5701 agagaataat caacaaagca tacatggcaa taccagttgg agaagtagtt gccaacatct
     5761 cttgtgctac ccttacccaa gcccaatcat gacctgtggc actcaaaaac tgaagaatag
     5821 ccgcaggcgc tgcagtaatc gaactggcaa agataacagg gataactcca gcaggattta
     5881 cctttaacgg aaggtaagag ctagatggag caccttgtgc aaccttagta tattggattg
     5941 gaattttgta ttctgcttgt tgaacataag ttgtaaagta aataatcaac aatacagtaa
     6001 taatcaaaat gattacgaaa atgatagatg aagtgatacg gctacttggg acgttcacaa
     6061 agtagtccac atagatgccc tgaatcatct ctggaattga ggaaacaatc ccggcaaaga
     6121 taatcatgga aacaccattt ccgtatccct tatctgtaat ttgctcaccc agccaagtga
     6181 caatcatact accagctgtt aagatgatac caatcgtcag aaaaacttgt ggagttaaag
     6241 cagttttaat caattgagct ccagccaagg tattaaaacc agctgtaatc ccgatagatt
     6301 gcacaaaagc gagaactaga gcaatataac gagtagcttg attcaatttt cttcgaccta
     6361 cttccccttg tttaccccac tctacaaact tgggtaaaat atccatttgc aagagttgga
     6421 caacaataga agcggtgata tagggactaa ctcctagggc aaaaatcgaa aagtttttta
     6481 gggcattccc cgacaccaag ctcaacatgt ttaagaagga taatccactt aaagcattca
     6541 agctattggc attcacacca ggaactgtaa tgctagttcc gatacgaaag accaaaacga
     6601 taaaaattgt aaataaaatt tttgatcgaa cctgcttgac tttaagagct tctcttaata
     6661 atttaaaaaa cataggtcac ctctcttaga tgacttctac tgaaccacct ttagcagtga
     6721 tagcttcttc agctgattta gagaatttag ctgctttcac agtcaatttc ttagtcaact
     6781 caccgttacc aagaatttta atacctgact tttcagcttt aacaattcct gcttcgataa
     6841 gaacaactgg agttacttca gcaccatctt caaagacgtt caattggtca aggttcacaa
     6901 ttgcgtattc tttagcgttg atgttagtga atccacgttt tggaagacga cggaacaatg
     6961 gagtttgtcc accttcaaaa ccaaggcgaa ctccgccacc gctacgagct ttttgacctt
     7021 tttgaccacg accagatgtt ttaccgttac ctgatgaagt accacgacca acgcggttac
     7081 gtactttacg agaaccttct gcaggtttca attcatgaag tttcattttt attttctcct
     7141 cttttgtaaa atgctagcgc cgataaggga gaaaaggttg tctcccccat caactcgcct
     7201 atacgatgtc atcatagatg actatatcta gttttagggg atggtacagt gcacatcccc
     7261 taaaacttca ttagtttact tcttcaactg ttactaagtg agatactgct gtgatcatac
     7321 cacggatagc agcgttatct tctttaataa cagagctgtt caatttgcca agtccaagtg
     7381 ctacaacagt tttacgttgt gatggaatgc gtccgattgg agacttagtc aaagtaattt
     7441 taatttgagc cattttatcc cctttcttat gccaaatcag aaactgaaat accacgaagg
     7501 gcagcaattt cttcagcgcg tttcaattgt ttcaaacctt caacagttgc acgaacaatg
     7561 ttgattggag tgttagaacc aagtgattta gatgtaatat ctgccacacc tgccaattcc
     7621 acaacggcac gaactgcacc accagcggca actccagaac cttctacagc aggtttcaac
     7681 aatactttag ctccaccgaa ttctgaaaga acttcgtgtg ggattgttgt tccaaccata
     7741 ggaacttcga tcaagttttt cttagcatca tctactgctt tacggattgc ttctggaact
     7801 tcttgagctt taccagtacc aaatcctacg cgaccattgt ggtcaccaac aacaacaaga
     7861 gctgcgaaac gaagacgacg tccaccttta acaacttttg taacacggtt gacagcaact
     7921 acgcgttctt ctaattcaac tgcattgtct ttaaatgcca ttttctagtg tcctcctatt
     7981 agaatttcaa tccgttttca cgagctgcat cagccaaagc tttcacacgt ccgtgatata
     8041 gatatccacc gcggtcgaac accacttctg aaataccttt agcgtttgca cgttctgcaa
     8101 cgagtttacc gacagcaacg gcttgttcag ttttagttcc ttttgaaact tctttatcaa
     8161 gagttgaagc acttgcgagc gttacacccg ctacgtcatc aatcacttga gcgtagatgc
     8221 ctgtattaga acggaatacg ttcaaacgtg ggcgatcagc agttccagag agttttccgc
     8281 gaacgcgacg gtggcgtttt tggcggagtt tgtttttatc tggttttgaa atcacagttt
     8341 tcacctcttt agttttaaat cgtgtgctat gcacaaagtt ggaaaatagg ttggtggttg
     8401 atgatcaacc actcaacatt atttacctgt tttaccttct ttgcggcgaa cgaattcacc
     8461 aacgtaacgg atacctttac ctttatatgg ttctggtgaa cgaaggctac gtacgtaagc
     8521 agctgtttga ccaactactt cttttgaaat tccgctaaca acgattgttg ttgggtttgg
     8581 aagttcaaaa gtaattcctt ctggagcttc aacttcgtct ggatgagatt taccaacagc
     8641 caaaacaagt ttagatcctt gaagctgtgc acggtaacca accccacgca tttcaagttc
     8701 tttcttgaat ccttctgata caccaacaac catgttgttc aaaagggcac gagtagttcc
     8761 gtggatagtt ttcatttctt ttgaatcgtt tggacggtga agagttactt cagtaccttc
     8821 cacacggatt tcaatatctt ttgagaactc acgagtaagt tctcctttag gtccttttac
     8881 agttacaacg ttgtcattgt tagcgagttc aacaccagca ggcaacacga taactttatt
     8941 accaatacgt gacatgttta ttttctcctg ttaaattgtc aggccaaaac ggccagtttt
     9001 cacggggttc agatacttat ttagttcaag agctgaactg aacacgctct aagaactgtg
     9061 caaaatagat aagttggctt gtttgtaaac aaacttctcc aacttcctaa ttttgctttg
     9121 ttctttacga gctttgtatc ttgattttac caaacgtaag cgataacctc accaccaaca
     9181 ttcttttggc gtgcttcttt atcagtaagc aaaccttcag aagttgaaag gatggcaatt
     9241 ccaagtccgt taagaacttt tggaaggtct tcacgttttt tgtagacacg aagtcctggt
     9301 ttagaaacac gtttcaagtt agtgataact ttctcaccat ttggtccgta tttaagaaat
     9361 acacggatga cgccttgttt gtcatcttcg atgatttcaa cgttttttac aaaaccttcg
     9421 cgtttaagga tttcagcaat cccttttttg atgtttgatg caggtacttc aagtacttcg
     9481 tgtttagctt ggttagcatt acgaatacga gttaggaagt ctgcgattgg gtcagtcata
     9541 accattttgt ttttctcctc ttactagtag tttgcaagtt gcacttgcta gttaatacat
     9601 gatacaaaga gcgtaacagc acgagcaaaa atagacaatt taatgcagaa gcaatgctcc
     9661 aaagcaaatt ggtctttttt gccaaagctg tagctcgggt tcaaattggc aagcccaatt
     9721 gaacccgggc taaactctgt gtgaaaaaga taaactttcc tagaaacttt agtttcttcg
     9781 tcaagtttcc tatttttact tggagttttg acacccttga tatcttaaat taccaagatg
     9841 cttttgttac accaggaatt tgacctttat gcgcaagttc gcgaaaagcg atacgactca
     9901 gaccaaattt gcggtaaact gaatgtgggc gccccgtaac tctacaacga ttatgtaaac
     9961 gagtcggtga ggcgttgcga ggtaatttag ataaaccttc gtagtcccct gccgccttta
    10021 atgcagcacg tttttcagca taacggtcaa caattttttg gcgtttagcc tctctagcta
    10081 ccattgattt tttagccatt agatttacct cctatattat tttgcaaaag gcattccaag
    10141 gcctgtaagc aatgcacgtg actcttcgtc agtgttagca gttgttacga taacgatgtc
    10201 aagaccacga gttttgtcaa cgtcatcgaa gttgatttct gggaagatta attgttcttt
    10261 cacaccaagt gtgtagttcc cgcgtccatc aaatgatttt gttgggacac cgtggaagtc
    10321 acgtacacgt ggaagtgaaa ctgataccaa tttatccaag aattcgtaca tacgttcacc
    10381 acgaagggta acttttgcac cgatcgcaac accttcacga agacggaagc cggcgattga
    10441 ttttttagct ttagtgataa gtggtttttg acctgagata agtgccaatt cttcagcagc
    10501 tttttcaagg cttttagcgt ttgatacagc ttcaccaaca cccatgttca aaacaatctt
    10561 atctacttta ggcacagcca tcactgatga gtagttgaat tgttctgtca aagcaggaac
    10621 tacttcatta agatattttt cttttaaacg atttgccatt atacttctcc tttccttcgt
    10681 gattaatcaa gcacttcgcc tgattttttg ttgtagcgaa cttttttacc gtctacaaat
    10741 ttgtaaccaa cacgaccagc tacaccattt ttgtccaaaa cttgaacgtt tgatacgtgg
    10801 atagctgctt ctttctcgat gataccacct tgaggaagct cgttagttgg acgttggtgt
    10861 ttcttaacaa tgttaacacc ttcaacgata actttgttta cttttggaag ggcagtaagg
    10921 acaacagctt ctgttccctt atctttacca gcgattacgc gaactttgtc gccttttttt
    10981 acaaacatta ggtttctcct tgatttttct tacgcccata agggcaccct agcttgaagc
    11041 taggggacta gtttgttcct aaaaattaaa gtacttctgg agcaagtgac acgatcttca
    11101 tgaagccacc ttcacgcaat tcacgtgcaa ctgggccaaa gatacgtgtt ccgcgaggag
    11161 ttttgtcttc acggatgata actgctgcgt tttcgtcaaa tttgatgtat gaaccatcag
    11221 cacgacgagc acctgattta gtacgaacga taactgcttt aacaacgtca ccttttttaa
    11281 ccgcaccacc aggagtagct tgttttacag atgccacgat aacatcaccg atgtttgcaa
    11341 atttacgtcc tgaaccacca agaactttga tagtcaagat ttcgcgagca ccgctgttgt
    11401 ctgcgacttt caaacgagtt tctgtttgaa tcatttcagt tttctccttt caggtttgat
    11461 tagatgatga ccgcttcttc aacaacttct acaagacgga aacgttttgt agctgaaagc
    11521 gggcgagttt ccatgatacg tacgatatcg ccttctttgg caacattgtt ttcatcatga
    11581 gctttgtatt ttttagagta gttaatacgt ttaccataga ctgggtggtt acgttttgtt
    11641 tcaactacaa ctgtgattgt cttgtccatt ttgtcagata caacacgtcc aacaagaact
    11701 ttacgattat tgcgttccat tgaaatttct ccttccctag tctattattt cgcttcagat
    11761 tgaactgttt tgatacgagc gatttgtttt ttaacttctt tcaagcgagc tgtttgttcc
    11821 aattgaccag tagcagcttg gaaacgaagt tcaaacaatt cttttttcaa ttcgttttcg
    11881 cgcttcgcga gttcttcttg agaaagacca cgaagttctt taacaaattc ttttacttca
    11941 ttaagtttca tgccttctcc ttattctgct tcacgtttta cgaatttaca tttaactggc
    12001 aatttgtggc tagcaagtcg aagcgcttca cgtgcaatct cttcagatac accagcgatt
    12061 tcgaacatca ctttaccacg tttaactggt gctacccaac cttcaggtgc ccctttacca
    12121 gatcccatac gcacaccgat agctttagca gtgtatgatt tgtgtgggaa gattttaatc
    12181 caaactttac caccacgttt catgtaacga gtcatggcga tacgagcagc ttcgatttgg
    12241 cggttagtga tccagtggct agttgtagct tgaagaccgt attcaccgaa tgctacttct
    12301 tttccacctt ttgcttcacc gcgcattttt ccacggaact cacgacggtg tttaacacgt
    12361 ttaggtacta acattggtta tttacctcct ttagtgtttt tacgagctgg aagaacttca
    12421 ccacggtaga tccatacttt aacaccaagt ttaccgtatg tagtatctgc ttcttcccaa
    12481 gcgtaatcga tatctgcacg aagtgtgtga agcggaacag ttccttcaga gtatccttca
    12541 gcacgggcga tatctgcacc gttcaaacga cctgatactt gagttttgat tcctttagct
    12601 ccagcacgca ttgcacgttg gattgcttgt ttttgtgcac gacggaaagc aacacgttgc
    12661 tccaattgac gagcaattcc ttcacctaca aggtgagcat ccaaatcagg ttgtttgatt
    12721 tcgatgatgt tgatgtgtac ttgttttcca gtcaatttgt taagttttgc acggagtgca
    12781 tcaacgttag caccaccttt accgataacc atacctggtt tagcagtgtg aagtgaaacg
    12841 ttaactttgt ttactgcgcg ttcgatttca atagttgaaa ctgctgcgtc agcaagttct
    12901 ttttgaacga atttacggat tgcaagatct tcatgaaggt aatccgcgta ttctttttca
    12961 gcataccatt tggcatccca atcacggatg atgccgacac gcataccaat tggatgtact
    13021 ttttgaccca cgattttacc tccttatttt tctgcaacag ctacagtgat gtgagctgta
    13081 cgtttgttga ttggtgaagc tgaacctttc gcacgtggac ggaaacgttt catagttggt
    13141 ccttcgtttg cgaatgcttc agatactacc aagttagctt tatccaaacc aaagttgttt
    13201 tcagcgttag ctacagctga gttcaaaact ttcaagatga tttcagcagc tttgtttgga
    13261 gtgaatgtca agattgcgat tgcatcggct acgcttttac cacggatgtt atcaagaaca
    13321 agacgtgatt tacgaggtga aacacgtact gtacgagcca ttgctttagc tgaagtaatt
    13381 tctgccattt atgttctcct tattttctac gtgttttctt gtcgtctgca gcgtgacctt
    13441 tgtaagtacg agttggtgca aattcaccaa gtttgtggcc taccatgtct tcttggatgt
    13501 aaacaggtac gtgtttacgt ccgtcataaa ctgcaatagt gtaaccaatg aaacttggga
    13561 agatcgttga acgacgtgac caagttttaa taactttttt cttttcgtcg ttagcttgag
    13621 cttcaacttt tttcatcaaa tgctcatcga cgaaaggtcc ttttttaaga ctgcgtccca
    13681 tttttatatt ttctccttta aatgttgtac cacagcggct tgcactccta tggagcgcta
    13741 ccgagctggc ggatttacta gttgcttaag cgactagttt aatattattt ctcgttgcga
    13801 cgacgaacga taagtttgtc agatttcgct ttcttgttac gagttttaag accaagagca
    13861 ggtttgcccc atggagtaga tggtgcttta cgaccaactg gtgctttacc ttcaccacca
    13921 ccgtgtgggt gatcgttagg gttcattaca gaaccacgaa ctgttgggcg gatacctttc
    13981 caacggctac gtcctgcttt accaaggttt acaagtccat gttgttcgtt tccgacaaca
    14041 ccaactgtag cacggcaagt tccaagaatc atacgaactt cacctgattg aagacgaaca
    14101 agaacatatt taccttcaga acccaatact tgagcagatg caccagcagc acgtaccaat
    14161 tcaccaccac gacctggttt caactcgatg ttgtgaatca aagtaccaac tgggatgtta
    14221 gcaagtggaa gagcgtttcc gactttgata tctgcttctg gacctgaaac gatacgttga
    14281 cctacttcaa gaccttttgg agcgatgatg tatgctttca caccgtcagt gtagtgtaca
    14341 agagcgatgt ttgcagaacg gtttggatcg tactcgattg ttttaacaac tgcttcaacg
    14401 ttgtctttat tacgtttgaa gtcaaccaaa cggtagaaac gtttgtgtcc accaccttgg
    14461 tgacgaactg tgatacgacc gttgttgtta cgaccagcct tgctcttcaa tgcaacaagc
    14521 aatgattttt caggagtgct tgttgtgatt tcagcgaaat ccaaagaagt catattacgg
    14581 cgaccgtttg ttgttggttt ataaacacga attcccacga tatttcctcc ttagattatt
    14641 cagcttcagc agcaaacaac tcgattgctt tagaatcagc tgtaagtgtg atgatagctt
    14701 ttttagtttt gttagtaaaa ccagtgtaac gtccaacacg tttagctttt ggttttacgt
    14761 tgattgtgtt aacattggca actttaacac cttcgaaagc agcttcaaca gcttgcttga
    14821 tcaaaagttt gtgtgcacga gtgtcaactt caaatacata tttccctgct tcaagttgag
    14881 ccattgagct ttcagtgatg acaggttttt tgataacatc atacaaattc attatgcaag
    14941 aacctcctcg attttagaga tagctgcttg tgtgacaaga agtttgtcgc tatttgcgat
    15001 gtcaagaaca cttgcagttg tagcagttgc aactttcacg tttggaaggt tacgagctga
    15061 aagagctgcg aattcatttc cttcttcaag gataacaaga actttagaat cgatgctcaa
    15121 tgctgcaaga acttttgcaa attcagcagt ttttggagct gtaaatgaaa gagcgtctac
    15181 agctacgaat ttgttttcag caactttttc agagtaaact gatttaagag ctaggcgacg
    15241 aactttttgt ggaagtttgt agccgtatga acgtggagtt ggtccgaaga caacaccacc
    15301 accacgccat tgtggtgagc ggatagaacc ttgacgagca cgtccagttc ctttttgacg
    15361 ccatggtttg cgtccaccac ctgatactgc agagcggttt ttaacagcgt gtgttccttg
    15421 acgaaggctt gcgcgttggc tgatgattac atcaaacaca actgattcat ttggttcgat
    15481 accaaatact gcatcgctaa gaacaacttg gccagcttct ttaccagttt ggtcaaataa
    15541 tgttacgttt gccattgtga ctgatttccc ctttctttat tatttaccag ctttaactgc
    15601 tgatttgata gtgataagag atttcttagc acctggtacg ttacctttga taaggataac
    15661 gttcttttct ggaacaactt gtacaacttc aaggttttga attgttacgc ggtcgccacc
    15721 catacgccct gcaaggtttt tacctttgaa tacgcggtta ggtgcaacag gtcccataga
    15781 acctggacga cggtggtaac gagaaccgtg agccattggt ccacgtgatt gtccgtggcg
    15841 tttgataaca ccttggaaac ctttaccttt agaagtacca gttacgtcaa caacgtctcc
    15901 agctgcgaat gtttcaactg taatttcagc accaacttcc aagccttcaa cgtttttgaa
    15961 ttcacgaatg aagcgcttag gagccgtgtt agctttcgct acatgtcctt tagcaggttt
    16021 gttgctcaat acttcgcgtt tgtcatcgaa accaacttgg atagcgttgt atccgtctgt
    16081 ttcaacagtt ttaacttgaa gaacaacgtt tggagttgct tcaataactg ttacagggat
    16141 caattcgcca gcttcagtga agatttgagt cattcccact tttttcccta agattccttt
    16201 tgtcatgaga aaatagttcc ttttctatat tttttattca aaaagttttt aacgagcgtt
    16261 ttttatgctc aagtctaaga tacaaagggc gtcaaactca aagtgaaaat aggaaactga
    16321 cacagtgtct agcagacgcg aggaagttta tctttttcac accgagttta gcccgtgttc
    16381 aattagattg aaacaccagc ctctgctctt ttatatttta gattaaagtt tgatttctac
    16441 gtttacacca cttggaagat ccaatttcat caaggcatca actgtttttt gagttgggtt
    16501 aacgatatcg atcaaacgtt tgtgtgtacg catttcaaat tgttcgcgag agtctttgta
    16561 tttgtgagtc gcacgaatga ttgtgtagag gctacgttca gttggaagtg ggattggacc
    16621 cgcaacttgt gcacctgtac gagtagctga ttctacgatt tttgcagccg ctgtgtcaag
    16681 cgtacggtgt tcgtaagctt tcaaacggat acggattttt ttgtttgcca tctttttctc
    16741 cttttcgtct atttaagata ataggctagc tccacaagaa aaccgacgcg gtgttgcgtg
    16801 gcaatgcaac cgagcgtgtc gcaacctctt gcatcaaaac taaggctgta atttacagca
    16861 ccataataga ataacacaaa tcccctgcga ttgcaaggga tttgttaggt tttttcgttt
    16921 ttttgggatg aaactgtttt cctacttttt ctttctaaaa ttagggattt tcttctatat
    16981 ttcttctata cataagttaa gtcctcttga catcaaattt atcttctgat tggtccacta
    17041 agatatccgc cttagactca gtttctttat agtatcgcag atactgctcc cgtctcatct
    17101 gatggctagc caatataaag gatgcatcgc gatttctcac agtcgtatct ctagcaaggc
    17161 gtcgctttaa ttcggtctcc tcatccgtgt agaaacagat ggttttttca aagagttcct
    17221 tgggtagaaa accaacagac atcccttcga caatcaaaat tggtttggct ccagacaaga
    17281 cctcactagc cttccaaggt tcttcaattg tcaagacatc catacccgcc tgcaaggcaa
    17341 ggatatctct ctgcaaactc tccagttcat gcgccactgg cagactggct gtcacctttt
    17401 gattcggcgc gtccttgggt actaccaaat agcgtccgga ggtgatatag ggatctgttt
    17461 ctagcaaatt tactgtagta gaatctaaag cttggtacaa ttcctgtgca aaggttgatt
    17521 tacctgaagc tccatgaccg tatattccca gtgtcctgac tttccccgtc tcgatctctg
    17581 agactagttg gtctactaag tcttttttct tcattctctc ttcacctgtt catagctttc
    17641 ttttccgtca ttgagcttgt cccaaatcac tacttgccca cttttgagcg atttttgcac
    17701 atcgataatt cgttggttag atgaacctcg aaactggagc ataagatttc tcttagttcg
    17761 atcatatctt ccatcgacaa gaatgtcaat cagtgacaag aattccagtt tatctggagt
    17821 ttccaacatc atttcttccc aagtgtagcc ggtccaggac cagatgtcct tgtctggcaa
    17881 ttccttccga atccgcttaa caagtggcaa gagaatccca gtattaagaa aaggctcccc
    17941 tcccaacaaa gtcaaacctt gaacataggg ctgagcaaga tctgccatga tctgctcttc
    18001 taattctgct gtatagggaa tgccagcatt aaaagaccaa gtcgcaacat tataacatcc
    18061 ctcgcagtga aacatacagc ctgatacata gagagagtta cgcacgcctt cgccgtccac
    18121 aaagttaaag gccttgtagt caatgatacg accttgacta agttcctcgc ttttccattc
    18181 ttgtggtttt ggattattca ttcgctacct ctatccaata acgctcgact ccattgcgag
    18241 catcctcaaa tattccacca tttgctagaa tgactgctct gctagcagga ttattcacgc
    18301 tacaggtcac cagagctttc ttgatgttct tttccttagc aacttgcaag ccctgacgga
    18361 gagtctcttt tgcataaccc ttgcctcttt cagatggacg aatggagtag ccaatgtggc
    18421 caccttcttc tagtagaaag ttactgaggc gcaaccggag attaagaaat ccaactgctt
    18481 gacctttctc agaaaaagcc actaactgaa ttgcaggaac ccatccttca ggcagattaa
    18541 tccccatttc ctgttcctga ttgctttcta accagtcttc atacacaaag ttctctgtat
    18601 cccagaaacc gccgtcgtga ggcgactgaa atttttcaaa ctctgtcatc atatctaaaa
    18661 cagctttctt atccgctaat cttggtctgc gtaattccat ccttacctcc cactatgaga
    18721 aaagcgagag ctggctctca cttttatttt ttcttgttct tgtttaaaaa ttgttctctg
    18781 aagctaagca agcgttcttt cttaccagct ccacctttag agtgtttctc gtgataacgg
    18841 gtcacttgtg cgcgcccctt atcgtctagt tgatattttc ccatttcatt tctttctaat
    18901 ttgttacttg atgcccagct attttaatcg ttgaaccatt catatgtttg acacgcgcag
    18961 cgatttcctt gtgacgcccg ttgaccatcg gtcttgcttg aggattacct aggtagccac
    19021 aagttcgctt caccacatct actgttttag ggtcgctatt gccacagttt ggacaagcaa
    19081 accctctctc agttggttca aaatcccctt caaagtcaca cttgtagcaa cggtcaatcg
    19141 gagtattggt gcctagatag cctacacggt cataagcata atcccagaca gcttccaagg
    19201 cctttggatt ttgctgaagg actggatact cacaataatg gatgaaacca cctgacgcac
    19261 ctgcttctgg atagactttc tcaaagtcca atttttcaaa cggtgttgga tttttacgaa
    19321 catcgtagtg gaaagagttg gtgtagtatt ccttgtctgt gatatcagga atagagccaa
    19381 acttgtctat atctagtcgg cagaaacggt ctgtcagact ttcggatggt gttgagtaga
    19441 tagagaaatg gtagccatat tggtctgacc actcttctac acggcgtttc atatcgtgaa
    19501 tgatgtctag cgtgaattcc ttagcatctg gattactttc ccagctgtta ccaaagaaaa
    19561 ctgtcgctac ttcatacaag ccgatatagc ccagcgaaac ggtcgcacga cgattcttaa
    19621 agagctggtc aacactttct tctttaccta gacgatggcc aaaagcaccg tactgataaa
    19681 gaataggagc attcgctggt gtcgcctctt tagtgcgttc gacacggtaa acaagagcat
    19741 cttctgcgat attcattcgc tcgttgaaga tttcccagaa cttattcata tcaccttcag
    19801 actcaagagc aatacgaggc agattaaccg tcacaacacc cagattcatg cgacctgaat
    19861 tgacttctac accattttca tccttccacc cttgaaggaa agaacggcag cccataggaa
    19921 ctttgaaaga acctgtcaaa tcaacaatct tatcataaga caagacgtct ggatacatcc
    19981 gcttagttgc acactctaga gccaactgct tgatgtcata gttgggagtt ccttcctcta
    20041 agttgaggcc tcttttaagc gtaaagataa gtttaggaaa gatagccgta cggtgttctg
    20101 aaccaagacc cttgatgcga atgtttaaaa tagctttttg aatttctcgt tcaaaacgac
    20161 tggttcccag accaaaacct aacgaagtaa aaggtgtttg tccatttgaa gtgaagagag
    20221 tattgatttc atactcaaga gattgcatgg catcgtagat gtccttttgc gctttcttcc
    20281 aagcgtaatc ttcccgtttt tcaggcaata cccactcttc tgcatctttg agatgttttt
    20341 gataattctt ctctgcataa ggcgccaaaa tttcatcgat acggtcagct gaacagccac
    20401 cgtactggct agaagcaacg ttggcaatga tttgagaaat ctgtgctgtc gcagtctgga
    20461 tagacttggg actctctacc tctgcatttc caatcttaaa accattttcc aacataccct
    20521 taaaatcaat caaacagcag ttggtcatag gggtataggg actgtagtcc aaatcgtgat
    20581 agtggatatc ccccttttgg tgggcattgg ctacgtgctt aggaagcatt tgcagtccga
    20641 ttgatttccc aacaatccct gctgtcaaat cacgctgagt gttaaagaca tcactgtctt
    20701 tattagcatt ttcattgaca actgtctggt ctttgttgag aagtttatga atactaaagt
    20761 tgatatccgt cgcttttgag cgctcaaaat ccctctgtgt ccgataagta atatactcct
    20821 cagccagtgc atattctttg gcttcaagga gttcatgttc tacgatattt tgaatttcgt
    20881 aaatcttaat tccctgtgga aagcgactat gaatttctgt aataattcgc tcagtcagat
    20941 cattgaggcg tttttcaacc aggggtgtca catccataac cttgtcagcc gccttgcgga
    21001 gagccttgtc aatcttgtcc acatcaaata caacacgtct cccatctcgt ttctcgacga
    21061 agagagttgg caaaattgta attttttctt ctagtgcaat catacgcatg ctcttttcta
    21121 aaatgttctt tctaaaaagt attataccac tctaaaaaga aa
//
LOCUS       DAVMAF010000028        21053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMAF010000028 DAVMAF010000000
VERSION     DAVMAF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.028"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..493)
                     /locus_tag="VYO97_001098"
     CDS             complement(<1..493)
                     /locus_tag="VYO97_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1753905.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNN"
     gene            661..1983
                     /locus_tag="VYO97_001099"
     CDS             661..1983
                     /locus_tag="VYO97_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1753906.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMVVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1985..3871
                     /locus_tag="VYO97_001100"
     CDS             1985..3871
                     /locus_tag="VYO97_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV1753907.1"
                     /translation="MVIRVFDKQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRRDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEISGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            4039..4383
                     /locus_tag="VYO97_001101"
     CDS             4039..4383
                     /locus_tag="VYO97_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1753908.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            4393..5805
                     /gene="lacG"
                     /locus_tag="VYO97_001102"
     CDS             4393..5805
                     /gene="lacG"
                     /locus_tag="VYO97_001102"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1753909.1"
                     /translation="MENLQFKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLDRENIDHFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKTGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5874..7553
                     /locus_tag="VYO97_001103"
     CDS             5874..7553
                     /locus_tag="VYO97_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1753910.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDTSAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QPISFVLLSLMLVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(7678..9117)
                     /locus_tag="VYO97_001104"
     CDS             complement(7678..9117)
                     /locus_tag="VYO97_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1753911.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLIFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(9121..10470)
                     /gene="trkA"
                     /locus_tag="VYO97_001105"
     CDS             complement(9121..10470)
                     /gene="trkA"
                     /locus_tag="VYO97_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1753912.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIEREHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            10659..11507
                     /locus_tag="VYO97_001106"
     CDS             10659..11507
                     /locus_tag="VYO97_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1753913.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            11522..12070
                     /locus_tag="VYO97_001107"
     CDS             11522..12070
                     /locus_tag="VYO97_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1753914.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            12077..12289
                     /locus_tag="VYO97_001108"
     CDS             12077..12289
                     /locus_tag="VYO97_001108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753915.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            12391..14073
                     /locus_tag="VYO97_001109"
     CDS             12391..14073
                     /locus_tag="VYO97_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1753916.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQYIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRII
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            14058..14888
                     /locus_tag="VYO97_001110"
     CDS             14058..14888
                     /locus_tag="VYO97_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1753917.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            15081..15584
                     /locus_tag="VYO97_001111"
     CDS             15081..15584
                     /locus_tag="VYO97_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1753918.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      15638..15872
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16037..16600
                     /locus_tag="VYO97_001112"
     CDS             16037..16600
                     /locus_tag="VYO97_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1753919.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            16614..17240
                     /locus_tag="VYO97_001113"
     CDS             16614..17240
                     /locus_tag="VYO97_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1753920.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKEPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            17268..17657
                     /locus_tag="VYO97_001114"
     CDS             17268..17657
                     /locus_tag="VYO97_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1753921.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            17662..18111
                     /locus_tag="VYO97_001115"
     CDS             17662..18111
                     /locus_tag="VYO97_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753922.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            18239..18826
                     /locus_tag="VYO97_001116"
     CDS             18239..18826
                     /locus_tag="VYO97_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1753923.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            19216..20823
                     /locus_tag="VYO97_001117"
     CDS             19216..20823
                     /locus_tag="VYO97_001117"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1753924.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6238 a         3601 c         4668 g         6546 t
ORIGIN      
        1 ggttgtttgt aatgatactt ccggagtctt tgttataatg tccaggtatt gataagccaa
       61 atccaagtaa ttttgtttcc aaagggacgt actctatagc tgtttttatc atattggtaa
      121 tggtggaatt gatttctgaa ataggataat cttcattcaa aatttctgtc tcgaaaagag
      181 ttttcccttg taagtcacat agactgagaa agatacctag gacgtttatt tcaatacctc
      241 caacaacata cggaatatta tctgtgatag aaatagtttt tctacgtctt ccagatccgg
      301 aaaactcgct aaattcatca ccagtttcgt atatgaggga ctgttcgctt agctctttta
      361 tgatgttagt cgttgttgct ggagtaaggc ctgtttcatt aacaatatct gtacgggatg
      421 ttgctggatt tcgatataga aactccagta tagtggctct attttgtaaa aaggctgatt
      481 gttttgtaat catttgtttc ctcctttggt ttattttata ttaaacataa gaaaaaatca
      541 attaaaatta ttattctata caaagaaaaa aatattgtaa aaacaacaaa aaatatattg
      601 acaacgcttt ctgttagtgg tatatttaat ttataaaaaa taaaaaaagg agttccggtg
      661 atgaacaata ttttagcgtt tttagaaaca aaagtcgctc cgtttggtga aaaagttggc
      721 aaccaacggc atttgaaagc tattcgtgaa ggatttatga tggcaatgcc tttgatttta
      781 gtcggctctt tatttcttat tctaatcagt tggcctcaag aggattttac aaattggctg
      841 aatagtgttg gattgctaag tatcttgaca actatgaatc agtcaacagt agcgattatc
      901 tccttggtcg cttgtttcgg tattgcctac aggttgtcgg aaggatatgg tacagatggt
      961 ccgtcggcag ggatcatagc cttatccagt tttgtattga tggcacctcg tttttcgagt
     1021 atggtttatg ataaaaatgg ggagcaggtc aagcagttat ttggcggcgc aataccattt
     1081 tctagcctga atgcatcttc tttgtttatg gcgattacta ttggattggt tacagcagag
     1141 atttatcgta tgtttatcca gcgcggaatt acgataaaaa tgccaagtgg tgtcccagat
     1201 gtggtaagta aatcattttc agctctttta cctggcttta ctacttttgt tttgtgggct
     1261 ctggtcttaa aaggtcttga agcggcagga gttgcaggag gtctcaacgg actcctaggt
     1321 gcaattgttg gaacaccgct taagttaatt gcaggaacgc ttccaggtat gattctatgt
     1381 gttattgtaa actcattctt ttggttctgt ggagttaatg ggggacaagt tttaaatgct
     1441 tttgtagatc cagtttggtt acaatttact acagaaaacc aagaagctgt ggctgcagga
     1501 caaacactcc aacacattat tacattaccg tttaaagatt tatttgtatt tattggtggc
     1561 ggtggagcga ctattggtct tgcgatttgt ctcttcctat ttagtaagag tcgtgcgaat
     1621 aaaacattag gtaagctagc tattataccg tctattttta atatcaatac agctattcta
     1681 tttacgtttc caacagtttt aaatccgatt atgctgattc cgtttattgc tactcctaca
     1741 atcaatgcct taattaccta tgtatcaatg gttgtaggat tagtacccta tacaacaggt
     1801 gtaatccttc cgtggacaat gccaccgatc ataggaggct tccttgcgac aggagctagt
     1861 tggcgaggag ctctattaca agttgttttg attttggttt ctgtagcaat ttattatcca
     1921 ttcttcaaaa ttgcagataa acgcaatctt gaaaaagaaa aagctactgt tggagggaaa
     1981 taagatggtt atcagagtat ttgataagca gaaaaatact tattctagct ttgccttaga
     2041 ggaattaagt tactatataa atcgggtctt taagactgac atagaacttg tcgaggagaa
     2101 ggaagcggat atttttgtag gattagtcaa taaagaggat agaagagacc atgttcttat
     2161 ctcattagac aagggtaagg ggagaattga gtctaataca attgtaggtt tacttattgg
     2221 aatttaccga atgtttcatg aatttggggt tgtgtatact agaccagggc gcggacatga
     2281 ctttgttcca gagttacgat ttgaagattt tttagataaa cagctatcta tagatgaaac
     2341 agccagttac tatcataggg gagtatgtat agagggagcg gattcatttg aaaatatact
     2401 agatttcatt gattggctac ctaagattgg gatgaacagt tttttcatcc agtttgaaaa
     2461 tccctactct tttttgaaac gttggtatga acatgaattt aatccatatc taaataaaga
     2521 acaattttca aatgaattag tacaagaatt gagtgatagg ttggataaag aattgcaaaa
     2581 aagaggtctt attcatcatc gtgttggtca tggatggaca ggtgaagttt taggttactc
     2641 ttcaaaattt ggctgggaat caggtcttag tatttcagag gagaagaaac cctatgtcgc
     2701 tgaaataagc gggaaacgag aattatttaa tacggctccg attttaacca gtctggattt
     2761 ttcaaatcca gatgtggcgg ataagatggt agaaattatc aaggattatg ccaagaaaag
     2821 acctgatgtt aactacttac atgtatggtt gtcggatgct cgtaataata tttgtgaatg
     2881 cgaaaactgt agacaagaat tggtttcgga tcagtatatt cgtattctca atcaattgga
     2941 tagggcttta acgagtgagg gattagatac aaagatttgt tttctgcttt accatgagtt
     3001 gctatgggcg cctcagaaag aaaaattaga taatcctgaa cgctttacca tgatgtttgc
     3061 accgattaca agaacatttg aaatgagtta tgcagatgta gattttgaca attccatacc
     3121 tacgcctaaa ccttatctgc gtaataaaat tatacttccg aattctcttg aggaaaattt
     3181 atcttatctt tttgagtggc aaaaaacatt taaaggagat agtttcgtat atgactatcc
     3241 tttagggcgt gctcattatg gcgatttagg ctatatgaaa attagtcaaa ccatttacag
     3301 agatgtatct tatctttcta acctacattt gaacggttac atttcgtgtc aagaattacg
     3361 tgccggattc cctcataatt ttcctaatta tgttatgggg gaaatgctct ggaagaagac
     3421 aagaagttat gaagaattga ttgaagaata cttttctgct ttgtatgggg aaaattggca
     3481 gtctgttgtt gaatatttag aaaaattatc catttattcc tcttgtgatt attttaatgc
     3541 aattggcagc cgtcaaagtg atgttttagc gaatcattat tatatagctt acaatctagc
     3601 tgataatttt ttaccaatta ttgaggaaaa tatttctaag ttattaaata gtcaaaagga
     3661 tgaatggaaa cagctcagtt atcatcgtga atatgttgtt aagatggcga aggctttata
     3721 tcttcaagca actggaaaaa caaggcaagc tcaagatgaa tggagaaatg tgttgaatta
     3781 tatccgtggg cacgaattgc tatttcaatc taatttggat gtttatcgtg taattgaagt
     3841 agcaaaaaat tacgctggtt tccacttata aatcataagt atagaaaatg aactaaggta
     3901 ttcagagaag attgacccta aatattatga aatttaagga tttttaagat atttagggtc
     3961 gactttctat ttgtatcgta gcgaagtcat tttaataatg atgtgtaaaa gatggatcaa
     4021 gattgaggag gaagaaagat gaaatcaaaa gaagaaataa atatgcttgg ttttacaatt
     4081 gtcgcttacg caggagatgc aaggtcagat ttgatggatg ctttggcgtt tgcgagagat
     4141 ggatattttg aacaggcaag agaattggtt gagtctgcaa acgactcaat agtgtctgcc
     4201 catcgagaac agactaattt attagcagag gaggcatatg gagataattt tgaagtgagc
     4261 tttattatga ttcatggtca agatactttg atgacaacga tgctattgta tgatcaggta
     4321 aagtttttta ttgatgaata tgaacgaatt cgaaagattg aagaacatat tggtttgcaa
     4381 tgaggattag tcatggaaaa tttacagttt aaagccttac cgaaggagtt tttattagga
     4441 gctgctaccg ctgcttatca agtagagggt gcaactaggg tagatggtaa aggaataaat
     4501 atgtgggatg tttatttgca agaaaatagt ccgttcttac cagatccagc tagtgatttt
     4561 tattatcgtt acgaagagga tatagctttg gcggcagaac atggtttgca ggctttgcgt
     4621 ttatctattt cttgggttcg tatatttcct gatatagatg gagatgctaa tatattggct
     4681 gttcgttatt accatagagt ctttcagtct tgcttaaaac ataatgtgat tccgtttgtt
     4741 tctttacatc attttgattc gcctcagaaa atgttagaaa caggggattg gttggataga
     4801 gagaatattg atcatttcat acgatatgct cgcttttgtt tccaagaatt tacagaagtc
     4861 aagcattggt ttacaatcaa tgaactgatg tctcttgctg caggtcaata tataggaggt
     4921 cagtttcctc caaatcatca ttttcaatta tctgaagcaa ttcaagcgaa tcataatatg
     4981 ttgttggcgc atgctcttgc agtcctcgaa tttcatcaat taggaattga gggagagata
     5041 ggttgtattc atgctttaaa gccaggctat cctattgatg ggcaaaaaga aaatattttg
     5101 gcagctaaac ggtatgatgt ttataataat aaatttctat tagatggaac ttttttgggc
     5161 tactacagtg aggacacgct ttttcacttg aatcaaatat tggaagctaa taattctagc
     5221 tttattattg aagatggtga tttagaaatt atgaagagag ctgcacctct taatacgatg
     5281 tttgggatga attattatcg ttcagaattt attcgtgaat acaaaggtga aaatagacaa
     5341 gaatttaatt caacaggaat aaaaggacag tcttctttta aattaaatgc tctaggtgaa
     5401 tttgtaaaaa aaactggtat tccgacaaca gattgggatt ggaatattta tcctcaaggg
     5461 ttatttgata tgttgcttcg tatcaaagaa gaatatcctc aacatccggt catttattta
     5521 actgaaaatg gtacagccct taaagaagtt aagccagagg gcgagaatga tattattgat
     5581 gacagtaaga gaatccgtta tattgagcaa catttacaca aagttttaga ggctcgagat
     5641 agaggagtca atattcaagg ctattttata tggtctttgc aagatcaatt ttcttgggcg
     5701 aatggctaca ataagcgata tggtcttttc tttgttgatt atgaaacaca gaagagatat
     5761 attaagaaaa gtgctctttg ggtaaaaggg ctaaaacgga attaaggtta gcgatttgac
     5821 tgatgtttaa tatgttttaa atatgaggtt gaatttttta taggaggagt tttatggata
     5881 agctagtcgc tgccattgaa aagcaacaag ggaaatttga aaaaatttct actaataact
     5941 atatgatggc tattaaagat ggattcattg ctactatgcc tttaattatg ttttcaagct
     6001 ttttgatgat tattattatg attcctaaaa atttcggagt agagttaccg agtccagcta
     6061 ttgtctggat gagaaaagtg tatatgttaa ccatgggagt tttgggtatt attgtttcag
     6121 ggactgttgg aaagtcatta gttggaaatg ttaacagaaa aatgcctcac ggaaaggtaa
     6181 taaatgatac ttctgcaatg ttggcagcca tatgtagtta tctggtatta actgtaacgc
     6241 ttgtagttga tgagaagacg ggatctacaa gtttgtcgac aaactattta ggatctcaag
     6301 gattgataac ttcgtttgtc agtgccttta ttactgtaaa tgtttaccga ttctgtatta
     6361 agcgagacat tactattcat ttacctaagg aagttcctgg ggctatatca caagctttta
     6421 gagatatttt tcctttttct tttgttttac ttattagtgg tttgttagat attgtatctc
     6481 ggtttagttt agatgttcct tttgcccaag tatttcaaca actattgact cctattttta
     6541 agggggcaga atcatatcct gctatgatgt tgatttggtt tatgtgtgct ttgctttggt
     6601 ttgttggaat tcatggacca tctattgtct tacctgctgt tacagcttta caactgagca
     6661 atatggaaga gaatgctcaa cttcttgcaa atgggcagtt cccttatcat tctttaacac
     6721 ctaatttcgg gaattatatc gctgctattg gaggaacggg ggctaccttt gttgtaccat
     6781 ttattttgat tttctttatg cggtctaaac aattaaaatc ggtaggtaaa gctacaatta
     6841 ctcctgtttt atttgcggta aatgaacctc ttctatttgg tatgcctgtt attttgaatc
     6901 cctatctttt tgtccctttt ttgatgactc caccagtaaa tgtatttcta ggaaaggtat
     6961 ttattgattt ctttggaatg aatggatttt atatccagtt accttgggct tttcctggtc
     7021 ccttgggatt gttaattgga acgaattttc aacctatctc ctttgtgctt ttatctttga
     7081 tgttagttgt cgacatattg atttatttgc cattctgtag agcgtatgat agacagttac
     7141 tgatgaaaga agatgttgca agctcaaatg atattatttt agaggaggat acaagtgaaa
     7201 taatgcctgg tgagatagat gtaataaaaa gtaaggagtt gaaagtactg gttctttgtg
     7261 cagggtctgg aacaagtgcg caattagcca atgcaattaa cgagggcgct caattagcag
     7321 aagttagagt gattgcgaat tcaggagcgt acggagctca ttatgatatt atgggtgttt
     7381 atgatttaat tattctggct ccacaagttc ggagttatta tagagagatg aaggtggatg
     7441 cagaaagatt aggtattcag atagttgcta ccagaggaat ggaatatatt catttaacaa
     7501 agagtccaag taaatcctta caatttgtat tggagcatta ccaagctgtg tagtaagttt
     7561 ttccatcttt tatttgagta agaactttgc ttacagacag gcttggattt aaaaacgttc
     7621 ccccttttta atataaaaat ccctctttca caattgtaaa aagagggatt ttgtatttta
     7681 tcttttagac caagttctct tcataaagag aagtaggatt gggtaaatct ccaagcggcc
     7741 tgcaatcatt gcaaaggaga ggagaatttt tgagatagga ctaaagattg agaaactaga
     7801 agtggttcct agaataggcc cgatattatt gaaacagcta aagacagcgc tggtcacgat
     7861 cagaaaatca ttgctatcta ggctgacaat aaagataagg gatagcaaaa tcatagcata
     7921 gatgacaaag tacttgagaa ttttatgctg ggtatcctta tcaatcaccg ttttattcac
     7981 atggagagtc aaaacacggt ggggagatag aatggacaaa atttgatttt tggcaatttt
     8041 tgaaaggatg aggcctcgaa taatcttgag tccacctgca gttgatccag cagaaccacc
     8101 gattgtcatg aggaagagga ggataaactg ggagaagagg ggccagttgg taatatctcc
     8161 atatccaaaa ccagttgttg taatgatgtt ggaaacctgg aagaaggtca tttcaaagct
     8221 ctttgaaaac cctgggtaga ggtagagggt gttgaggcta atcaagcctg tagaaaccag
     8281 tacaatgacc aagtaagccc gaagttcttc gtcaccaaag aaggccttga tgcgacggag
     8341 catgaggtag tagtagaggt tgaaatttac cccaaaaatc agaactccga tactgaccag
     8401 ataggtaatc agtgagctgc catagtgggc aattccgtcg ttatagacgg taaagcctcc
     8461 agttcccgct gtccccatag caatgacaaa actatcgtag agaggcatac cagctagata
     8521 atagatgatg acaaagaggg agaagagagc tagataaagg agatagagaa tctgggcagt
     8581 gttttttagt ttggatacaa ccttgccaaa aacaggacct ggaacctcag ccttcatcac
     8641 ctctaggtgg ctatttttgg cattgtccat aatagcaagt gcaaaaacaa gcactcccat
     8701 ccctccaatc aagtgggtaa aacttctcca gaagaggagg gaacggctga gaaccgaaac
     8761 gtcgttcaaa atacttgctc cagtagttgt aaacccagaa ctaatttcaa aaaaggcatc
     8821 aataacgcta gggatttgcc cagaaaagac aaaggggaga ccaccaaaga aagaccaaag
     8881 gatccaacag agggcaacga tcaagactcc ctccttggca taaatccgtt gattttttgg
     8941 cttctgtaaa ctccctgaac cgcctaacaa tacgagaatc cctatggtcg aaaagagggc
     9001 cgtaaagact tggctcgatt cacggtaata gacagcgata gccacaggaa ccaagaggag
     9061 aacagcttca atcaaaagta actttgaaag gaggtaacga atcatacttt tattcatttt
     9121 ttacctcgca atcaagtcat aaatcttggt gatgtttggc aacaaggttg ttactaggag
     9181 cttgtctcca acttccaaca tatcctcccc agttgggaaa atagtcttgc cctttcgaat
     9241 aatggctgca ataagaaccc cttttttcaa tttcagttga gaaagaggtt tggcagtcat
     9301 tttattggct tccttgatat ggaattgcag ggtttcgatt tggccattgg ctagatggtg
     9361 catggcttga aggtctgaat actgggcatt aactcgacca cgaataaagt gcataatcgt
     9421 atctacagcg atgcttttag gtgtgatgat acttgaaaaa tcaggcgcat tgataatctc
     9481 gaggagactg gtacgattga ccttagtaat atttttctgt acacctaccc tgtcaaggaa
     9541 catagatgta atcagatttt cctcatcgac acctgttaga gtcgcaacgg catcatagtg
     9601 ttgagcactt tcttccagca ggatatcttt tgcggttcca tctccttgaa cgatgtagag
     9661 atttgggaat ttctcgctaa agaagctggc gatttcagga ttgatttcaa tgacttttgt
     9721 atcgatacga ctatctttga gaataccaag tagataatag gcaattctac ctgccccaac
     9781 gatgagaagg ctcttcacgg cgcgtgattt aaaataatta tggaagagta tcatatcgac
     9841 acggttacca gtgacaaaga ttctatcttt atcctgtaca gtcatgtcac cgcttggaat
     9901 gataatttga tgctccctct ctatcgcaca gacaatgaca ttaccaaatt ttttacgaaa
     9961 atcagaaatg ggcatttggc aaagaccgct ggtggacttg acgacaaatt ccatgaggct
    10021 aacgcgtcca ccagcaaagc gttcgacaga cagggcgttg gggaagtcaa tgatattcgc
    10081 gatagcgcgg gcagccaaga gctcaggatt aacgataaga gaaaaaccga gaatattctt
    10141 ttccttgaaa taagagttag aatattcagg gttccgcacc cgaacgatag tttctttagc
    10201 tcccattttc ttggctagaa ctgctgcaat catgttgact tcatcgtgct cagtcagggc
    10261 gataaagata tcacaatctt ggacgctggc ttgctcaaga atggcaaaat cggccccgtt
    10321 accaaggata ccaatgatat caaagcgact gacaatatga ttgagaacag cttcgtcttg
    10381 ctcaatcagc aaaacatcat gcttttctgc aaccaaggag cgacagaggg caaaaccaac
    10441 ttttccccct ccgacaagga taattttcat aataaaacct actttttcat gatgtaacta
    10501 tcataccctt tttcaagaaa aaatgcacct actagctaat aaccagagtt tttagtagga
    10561 aatttgctat aagttaaaac tataccctaa ccaattgaaa tagctattag cgactttctc
    10621 tgaaatatgg tatgataaag gatatacaag gagataaaat gaataataat ttactggtat
    10681 tacaatcaga ctttggtctg gttgatggtg cggtatcggc tatgattgga gtggctttag
    10741 aagagtctcc aaccttaaaa atccatcact tgacgcacga tatcacgcct tataatattt
    10801 ttgaggggag ctatcgtctc tttcagacgg tggattactg gcctgaggga acgacgtttg
    10861 tatcggttgt cgatccaggt gtcggttcga aacgtaagag tgtagttgcc aagactgcaa
    10921 aaaatcaata cattgtcacg ccagataatg ggacgctttc ctttatcaag aaacacgttg
    10981 gcattgtagc cattcgtgag atttctgagg tggccaatag gcgtcaaaac acagagcatt
    11041 cttatacctt ccacggtcgt gatgtctatg cctatactgg tgctaaactg gccagtggtc
    11101 acattacttt tgaggaagta gggccagagc tcagtgtgga acagattgta gagcttccag
    11161 tcgtagcgac catcatagaa gatcatctgg tgaagggagc cattgatatt ctggatgtgc
    11221 gtttcggttc gctttggacc tctatcacac gggaagaatt ttacaagctg gaaccagaat
    11281 ttggtgatcg ttttgaagtg accatctatc atgctgatat gctggtctat caaaatcagg
    11341 ttgtctatgg caaatcattt gcagatgtga gaattgggca acccatcctt tacatcaact
    11401 ctctctatcg tttaggtttg gctatcaacc aaggttcctt tgccaaggcc tataatgtag
    11461 gtgtcggttc atcttggacc attgaaataa agaaaataga aggataaaat aggagaatag
    11521 tatggaaatc aaatttacaa ttaaacaagt tgttgctgtc ggaattggcg ctgccctctt
    11581 tgtcgtcatc gggatgatca acattccaac ccctgttcca aatacaagca tccagcttca
    11641 gtatgcggta caagcgctac tttctattat ttttggaccg attatcggtt tgcttgtcgg
    11701 gttgattggt catgcaatca aggactctct tgttggttat ggtctatggt ggacttggat
    11761 tatcgctagc ggactctttg gtttagttgt gggacttttt agaaagtacg ttcgcgtgat
    11821 caatagtgtt tttgactgga aagatattct tatttttaat ctcattcaac tacttgcaaa
    11881 tgcccttgtt tggggtgtct tggcaccact tggagatgtt gtgatttatc aagaagcggc
    11941 agaaaaagta tttgcacaag ggattgttgc gggaattgcc aatggtgtat ctgtagctat
    12001 tgcaggaact cttctcttac ttgcctatgc aggaacccaa actcgtgcag gaagtttgaa
    12061 aaaggactaa tactcaatga aaatcaaaga gcaaactagg aagttagccg caggttgctc
    12121 aaaacagtgt tttgaggttg tagatgaaac tgacgaagtc agtaaccata cctacggcaa
    12181 ggcgaaactg acgtggtttg aagagatttt cgaagagtat aaaatgatgg ggaaggctgg
    12241 gcaactagtc tttttcgatg tttatagatt agttaggcaa gtaagctagg ataggaatct
    12301 ttttggcgct aagattttaa gagaagagac tgcaagaagg gcttgattta tgataaaata
    12361 gaagtctaag aagcgaatga agagagtaag atgaaagaag ctataattga gtggaaggat
    12421 ttctctttcc ggtatgaaac acaacaagaa ccgaccttgc aagggataga cttgaccatt
    12481 tacaagggag agaaagtctt aattgttgga ccatctggat caggtaaatc taccttgggt
    12541 cagtgtttga atgggattat tcccaatatt tacaagggtc agacatatgg agaatttttg
    12601 ataaagggtc aaacagcctt tgatatgagc atctatgata agtctcatct ggttagcaca
    12661 gttttgcagg atacagatgg gcagtttatt ggcttgtctg tggcagaaga tttggcgttt
    12721 gctctggaaa atgatgtgac agccctagat gagatgaaag gtcgtgttta taaatgggct
    12781 gaaaagctgg accttcttcc tttactggat cagcgtcctc aggatttgtc aggtggacaa
    12841 aagcagcgag tcagtctagc tggtgtcttg attgatgaaa gtccgattct cttgtttgat
    12901 gagccactcg ccaatctaga tcccaagtca ggtcagtata ttatcgaatt gattgaccag
    12961 attcataagg aagaggggac gacgactctg attatcgagc accgtttgga ggacgttctg
    13021 catcgccctg tggatcggat tatcttgata aacgatggtc gtatcctttt taatgggagc
    13081 cctgaccagt tgcttgcgac tgatttattg actcaaaatg gaattcgaga acccctttat
    13141 ctaacgactc tccgtcaatt aggtgtggac ttagtcaagg aagaacagtt agcgaatctg
    13201 gataacatgt ctatctcaaa aggtcaggtt cagctgcaga atgaactggc aaaagaaact
    13261 ccagaattgc agtcactctt taaattagag gacgtgtctt tctcttatga tgatagaccg
    13321 attttaaaat ccctacattt agatattaaa aagggtgaaa agattgctat tgtcggaaaa
    13381 aatggagcag ggaaatcaac tctagccaag gctataagta gctttattca gacggaagga
    13441 cgctatcttt gggaaaaaca ggatataaaa ggcgattctg ttgcagagcg ggcggaacga
    13501 gtaggctatg tgctacaaaa tcctaatcaa atgatttcaa ccaatatgat ttttgatgag
    13561 gtggctctag ggctccgttt gcgaggtgtg gatgagaagg aaattgaaac gagagtctat
    13621 gaaaccttga aaatctgtgg actttatgaa ttccgtaatt ggcctatttc tgccctgtca
    13681 tttggtcaga aaaaacgtgt caccattgct tcaattttgg tcttaggagc tgaaattatt
    13741 ctcctagatg aaccgactgc aggtcaagat cagaagaact atactgagat tatggaattt
    13801 ctcgaagagt tacatcaaaa agggcatacc attgtcatga ttacccatga tatgcaattg
    13861 atgctggatt attcagaccg ggtccttgtc atggtggatg gagaattgat tgccgatact
    13921 gttccagcca gtctgttgag cgatcctgag ctgttagtaa aagccaatct aaaagaaacc
    13981 tccatcttta acttggctaa gaaactagat gtggatccac tggatttaac ggcattttac
    14041 aaagaaagga gagagggatg caagctaaat taatcggtta ccagcataga gatactgtga
    14101 ttcatcgctt gtcaggagct gggaaacttc tctttttcat tctggtatca ttggcggcca
    14161 tgattagcta tgataccaga atgctcttgc tgattgccat cttttcagtc tttctcctct
    14221 atttatcaga aattcgcttt aaagatgttt cctttgtagc cgtttttgcg acggtatttg
    14281 ccgttttaaa cgttttgatg gtctatctct tttctcccga gtatggggtt ggactttatg
    14341 gagagagaag tgtgatttgg cagggaatcg gtgtctacac tctaaccagc caggagctct
    14401 tttatctgct aaatttggcc attaagtatc tttgcaccat tcctctggct attatctttt
    14461 tgatgacaac ccatcctagt cagtttgctt ccagtttaaa tcaaattggt gtgccctata
    14521 agattgctta ttctgtcagc ctgaccttgc gctatattcc agatttgcag gaagaattct
    14581 ttactatcaa gatgtctcag gaggcgcgtg ggatggaatt atccaagaaa gcttctctta
    14641 tgcaacgaat caaaggcaat ctgctcatta ttacgccctt gatttttagc tcgctagaac
    14701 gcattgatac cattgcgact gccatggagc ttcgccgctt tgggaaagag aaaaaacgca
    14761 catggtatag ttatcaggcc ttgaaaaaag gagactatat taccttgctc ttggcaacct
    14821 tgtttttagt agttagttta ctacttatct tgcagaatca gggacgattt tacaaccctt
    14881 ggaaatagct tgaaaaaatt gaaaaaatca agtcatttct attgacaatg attctgaaag
    14941 tgttatacta agaaagtagt ttcgctgatt tacttcaaac ctgttgtgag gtaagttaac
    15001 gatgccttaa ccacgctgtt tgctgagctt gactccgggc agtgtggcta tttttttgca
    15061 atggtgaaag gaagcaagtc atgacaaatc acattgtatt atttgaacct caaattccac
    15121 aaaatacggg caatatcgcg cgtacttgcg ctgcgaccaa ttctcccctc cacatcatca
    15181 agcctatggg ctttcctatt gatgaccgca agatgaagcg ggctggattg gattactggg
    15241 ataagctaga gatttatttt tacgagagtt tggaagattt catatctcag atgaagggaa
    15301 aactctatct gatttcgaaa ttcgcggaaa aagtctattc tgaggtggat ttatcgactg
    15361 acgaggacca ttattttctc tttggacgtg aagacaaggg cttacctgaa gattttatgc
    15421 gagaacatcc tgagaaggct ctccgtattc ccatgaatga tgaacatgtc cgcagtctca
    15481 atgtgtctaa taccgtctgc atgattgtct atgaggctct tcgccagcag aactttgcag
    15541 gtcttgagct cgtccatacc tatgaagtgg ataaattgaa ataaaaaatg aaaatgaaca
    15601 aaatgcttgc gcttgcaagc gttttttgtt atgataaaag agtcttcagg gcagggtgtg
    15661 attcccgacc ggcggtgact ttaactagga aatgatcttt tccttttata ctttgttgac
    15721 aagctttgcc taaccagaag ttatgcctac agcttgtcgc ctagtctaaa agaaaaatct
    15781 ctatttcctt ctctttaaag aagtccgcga gcgcaagctg atgtggtgag attccacaac
    15841 cgacagtata gtctggatgg gagaagacga aagaatagct ttgtctgttt tgatagattt
    15901 atagccggat tgtaaccact tgctttgttt ttctttaata gagtaagagg gaacttttgg
    15961 gatataaaaa gtgagaatag atagaggaat cctttccaac ttcttctgat tttatagaaa
    16021 attggaggaa cctgttatga caaacacacg tcgactttcg accattgcaa ttttatcagc
    16081 catctcattt gtgctgatgt actttgactt tccgctttta ccagcggcat ctttcctcaa
    16141 gatcgaattt agtatcttgc cagtccttgt gggtctggtg gtcatggatt tgcctgctgc
    16201 tctaggagtg ctcttgcttc gctcactatt gaaattgctt cttaacagcc aaggagtgaa
    16261 tacttacatt ggtttgccga tgaatatcgt agctttggga gtttttgtca tcgtatttgc
    16321 tttgatttgg aaaaaggaac ggacaaccct tcgtttccta ctaggctctc tagctggaac
    16381 tgttggttta accttggcca tgttggttct caactatgtt tatgctgttc ctttgtacgc
    16441 taagtttgct aactttgata ttggaaaaat tttgggactt tccaactacc taatgaccat
    16501 ggtattacct tttaacttga ttgagggtgt aatcttttcc gtttcattct ggttgttgta
    16561 cgtccttttg aaaccaacct taaaacacta tgaaagataa acaaacattt ttaatgaagg
    16621 gcagttttgc ccttttactt ttcgttattc ttggctatat ggtcaaattt taccctgaaa
    16681 tgttggtcaa ttttgaccaa tcgattcaga ctgccattcg aggagactta ccagattatt
    16741 tgactattct ttttcgatcc ctcacacgtc tgattgatat cccagtgatt atcacttggg
    16801 ttgtcattac agcttttgtc ttttatcgta agcgatggaa gatagaaagt ttcttcatgc
    16861 tgggaaatct ggctttggca ggtcttttaa tcgtgacctt taaaaatatc taccagcgcc
    16921 cacgaccagc tatcttacat ctggtggagg agaagggatt ttccttccca agtgggcatt
    16981 ctctggctgt aaccttgatg gtaggtactt tgattgttat tcttagtcag cggattaaag
    17041 agccagtctg gagaaaaatc gtacaaatcg tccttggcct ctacctagtc agtgtgctgg
    17101 tatcaagagt ctatctggga gttcattacc catcagacgt ccttgccagt ctctgtgtgg
    17161 gcttgggagt cctgtttatc gagtttccct tctatgacaa gctccgcttc caatggcgat
    17221 ttaaaggcaa acagaagtga gtatcgaatt cccttgagga gaaagaaatg aaagtcaata
    17281 tagcagatct tcatccgact caactatatt tatcagaaaa gaagttgcaa gatattcaga
    17341 tgctttacca gtcggcagaa acaatccaag tcgatccaat cagtattctt gcctttggag
    17401 attgtttgct gattacagat gggcatcaca gggcttatca ggctttattg gcaggtcggg
    17461 atacgatttc tgctgagtgg gatagagatg gtggtgatga actatatcat ctctatgcgc
    17521 aagcttgcga ggaaagaaag atttactctg tttttgattt agaagatcgt atcctagctc
    17581 aagatgggta tgaagcaaaa tggtataact ggtgtgatgg ttttaatcaa gcagcaactc
    17641 ttttgttgaa aaggtagtgc attgtgtttt gttccttatt ataaggtcaa ttattgtgag
    17701 gaagcttttg cttggtatca ggatgtgaac ttggtttacc tcgtagatgg ggtgaagctt
    17761 ccttacagtc aagcgacctt ggaagccatg tattcctatt tggatcggca tggtgagctt
    17821 ttttggattg aagtcaagga gaagggagaa tggtttccaa ttggggatat tacactatct
    17881 caggataatc tccccattgt gattgggaat tccgcttacc aacatcgagg acttggaaaa
    17941 aagattctaa gtgctttgat tgaattggct cgagtaaaag gatggaaaga attgagagtc
    18001 aaggaaatct acacctacaa tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg
    18061 gaaaatggag caacagaaaa aggaaggagt tttatattgg aattagtcta atcctgccac
    18121 tttctccccc ttaacctttg actattccgc aaaattatcg taaaataaag agtaaatgat
    18181 aaaatgaggt cagagtctgt tcgctctggc gatagtagta taaatgagga gaaacgcttt
    18241 ggaattagaa gtatttgctg ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg
    18301 tgctatcttg gaacttcgtg gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga
    18361 aattcaaaac taccttggaa catcaaacag cgatatccgc gaagctttgc ctttgttcta
    18421 cacagagttg aactttgacg gtagcttcat ctcacttggg gacaacaaat ggggtcttcg
    18481 ttcatggtat ggtgtggacg aaatcgacga agaaatcatc gctcttgaag aaaatgacga
    18541 cgatgaagta gcaccaaaag ctaagaaaaa acgtgtcaat gcctttatgg atggtgattc
    18601 agatgccatt gactacaatg cagatgatcc agaagacgaa gatgcatacg aagcagatcc
    18661 agctctttca tacgatgatg aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga
    18721 tgcagaaatc aacgaaatcg ctccagatga cttgggagaa gatgtggatc tcaacgaaga
    18781 cgacgacgag ttttcagatg acgacgctga aaccagcgag gaataagagt tagctattga
    18841 caattatcct atttttaggt atcatattgt ttgggcacct cttttagagg tcggggctcc
    18901 ctagttctta gggagctatt tttatttttt caagaagtta tcttcttgta ttttatactc
    18961 aatgaaaatc aaagagcaag ctaggaaact agccgtaggc tgctcaaaac actgttttga
    19021 ggttgtagat aagactgaca aagtcaggaa cacatatcta cggcaaggcg acgttgacgc
    19081 ggtttgaaga gactttcgaa gagtattagt tgtgaatctg gtgcagttgt cccagattat
    19141 tcttattagt agggtcttgt tttctatatc ccctcgtagt taacaagacc ttgagcattt
    19201 tagaaagagg aatctatgtc tacgaaatat atttttgtaa ctggtggtgt ggtatcgtct
    19261 attgggaaag ggattgtggc agcgagtcta ggccgtctct tgaaaaatcg tggtctcaaa
    19321 gtaaccattc aaaagtttga cccttatatc aatattgatc cgggaaccat gagtccttac
    19381 cagcacgggg aagtttttgt gacagatgac ggagctgaga cagatttgga cttgggtcac
    19441 tatgaacgtt tcatcgatat caatctcaac aaatattcca acgtgacaac tgggaaaatt
    19501 tatagtgaag ttcttcgtaa agaacgccgt ggagaatacc ttggggcaac tgttcaagtc
    19561 attcctcata tcacagatgc tttgaaagaa aaaatcaagc gtgccgctct aacgaccgac
    19621 tctgatgtca ttatcacaga ggttggtgga acagtaggag atatcgagtc cttgccattc
    19681 ctagaggctc ttcgtcagat gaaggcagat gtgggtgcgg ataatgtcat gtatatccat
    19741 acaaccttgc ttccttacct caaggctgct ggtgaaatga aaaccaaacc aacccaacac
    19801 tctgtcaaag aattgcgtgg cttgggaatc caaccaaata tgttggttat tcgtacagaa
    19861 gagccagctg gtcaaggaat taaaaataaa ctggcccagt tctgtgatgt ggcaccagaa
    19921 gccgttatcg aatcgttgga tgttgaacac ctttaccaaa ttccactgaa cttgcaggca
    19981 caagggatgg accaaattgt ttgtgatcat ttgaaattag acgcaccagc agcggatatg
    20041 acagaatggt cagccatggt ggacaaggtc atgaacctca agaaacaagt taagatttcc
    20101 cttgttggta agtatgtgga gttgcaagat gcctatatct cagtggtcga agccttgaaa
    20161 cactctggct atgtcaatga tgtagaagtt aaaatcaatt gggtcaatgc caatgatgtg
    20221 acagcagaga atgtagcaga actcttgtct gatgcggacg ggatcatcgt accaggtggt
    20281 tttggtcaac gtggaacaga agggaaaatc caagccatcc gctatgcgcg tgaaaatgat
    20341 gttccaatgt tgggagtctg cttgggaatg cagttgacat gtatcgagtt tgctcgtcac
    20401 gttttaggtc ttgaaggtgc caattctgca gagcttgcac cagaaacaaa ataccctatc
    20461 attgatatca tgcgtgatca gattgatatt gaggatatgg gtggaaccct tcgtttggga
    20521 ctttatccgt ctaagttgaa acgtggctct aaggctgctg ctgcttatca caatcaagaa
    20581 gtggtgcaac gccgtcaccg tcaccgttat gagtttaata atgccttccg tgagcagttt
    20641 gaggcagcag gttttgtctt ttcaggagtt tctccagaca atcgtttggt agaaatcgtg
    20701 gaaattcctg aaaataaatt ctttgtagct tgtcagtatc accctgaact gtcaagccgt
    20761 ccaaaccgac cagaagaact ctacactgcc tttgttactg cagcggttga gaacagcaat
    20821 tagcaaaatc agaacctttg agaaaaatct cagaggtttt ttgcatacga tgatattgca
    20881 gtatatctga ggtaggggtc ctctgtatgt acctgctacc gttgaaatca atagcgactc
    20941 cctcttgccc tgtgctagtg aatggattta tcagtatatt gaaatgaaat aaaatttgaa
    21001 caaattaatt cggaaagcca aatcaatttc tagcaaagtt ttaggaactg gat
//
LOCUS       DAVMAF010000029        20858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMAF010000029 DAVMAF010000000
VERSION     DAVMAF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.029"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..130)
                     /locus_tag="VYO97_001118"
     CDS             complement(<1..130)
                     /locus_tag="VYO97_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEV1753925.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSY"
     gene            complement(344..1966)
                     /gene="rlmD"
                     /locus_tag="VYO97_001119"
     CDS             complement(344..1966)
                     /gene="rlmD"
                     /locus_tag="VYO97_001119"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1753926.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDHVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIELREH
                     YNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2040..3014)
                     /locus_tag="VYO97_001120"
     CDS             complement(2040..3014)
                     /locus_tag="VYO97_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1753927.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3088..3702)
                     /gene="pmp23"
                     /locus_tag="VYO97_001121"
     CDS             complement(3088..3702)
                     /gene="pmp23"
                     /locus_tag="VYO97_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1753928.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3702..4676)
                     /locus_tag="VYO97_001122"
     CDS             complement(3702..4676)
                     /locus_tag="VYO97_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1753929.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4684..5940)
                     /locus_tag="VYO97_001123"
     CDS             complement(4684..5940)
                     /locus_tag="VYO97_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1753930.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6002..6433)
                     /locus_tag="VYO97_001124"
     CDS             complement(6002..6433)
                     /locus_tag="VYO97_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753931.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6435..7037)
                     /locus_tag="VYO97_001125"
     CDS             complement(6435..7037)
                     /locus_tag="VYO97_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1753932.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7021..7860)
                     /gene="prmC"
                     /locus_tag="VYO97_001126"
     CDS             complement(7021..7860)
                     /gene="prmC"
                     /locus_tag="VYO97_001126"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1753933.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7860..8939)
                     /gene="prfA"
                     /locus_tag="VYO97_001127"
     CDS             complement(7860..8939)
                     /gene="prfA"
                     /locus_tag="VYO97_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1753934.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8949..9428)
                     /locus_tag="VYO97_001128"
     CDS             complement(8949..9428)
                     /locus_tag="VYO97_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1753935.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9418..10017)
                     /locus_tag="VYO97_001129"
     CDS             complement(9418..10017)
                     /locus_tag="VYO97_001129"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1753936.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10140..10322
                     /locus_tag="VYO97_001130"
     CDS             10140..10322
                     /locus_tag="VYO97_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1753937.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10458..11831
                     /gene="mnmE"
                     /locus_tag="VYO97_001131"
     CDS             10458..11831
                     /gene="mnmE"
                     /locus_tag="VYO97_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1753938.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDIEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11878..12813)
                     /gene="dapA"
                     /locus_tag="VYO97_001132"
     CDS             complement(11878..12813)
                     /gene="dapA"
                     /locus_tag="VYO97_001132"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1753939.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12869..13945)
                     /locus_tag="VYO97_001133"
     CDS             complement(12869..13945)
                     /locus_tag="VYO97_001133"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1753940.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14531..14603)
                     /locus_tag="VYO97_001134"
     tRNA            complement(14531..14603)
                     /locus_tag="VYO97_001134"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14568..14570),aa:Thr,seq:ggt)
     gene            complement(14703..15212)
                     /locus_tag="VYO97_001135"
     CDS             complement(14703..15212)
                     /locus_tag="VYO97_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1753941.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15210..15306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15335..15766
                     /locus_tag="VYO97_001136"
     CDS             15335..15766
                     /locus_tag="VYO97_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1753942.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15848..16225
                     /gene="mscL"
                     /locus_tag="VYO97_001137"
     CDS             15848..16225
                     /gene="mscL"
                     /locus_tag="VYO97_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1753943.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16267..17361)
                     /gene="hemH"
                     /locus_tag="VYO97_001138"
     CDS             complement(16267..17361)
                     /gene="hemH"
                     /locus_tag="VYO97_001138"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1753944.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSKQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTIRINENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17516..18739
                     /gene="pepT"
                     /locus_tag="VYO97_001139"
     CDS             17516..18739
                     /gene="pepT"
                     /locus_tag="VYO97_001139"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1753945.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18775..19404)
                     /locus_tag="VYO97_001140"
     CDS             complement(18775..19404)
                     /locus_tag="VYO97_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753946.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            19369..19590
                     /locus_tag="VYO97_001141"
     CDS             19369..19590
                     /locus_tag="VYO97_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753947.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19667..20208)
                     /locus_tag="VYO97_001142"
                     /pseudo
     CDS             complement(19667..20208)
                     /locus_tag="VYO97_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20312..20858)
                     /locus_tag="VYO97_001143"
                     /pseudo
     CDS             complement(20312..20858)
                     /locus_tag="VYO97_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6028 a         4380 c         3917 g         6533 t
ORIGIN      
        1 cataactatt tgcctctata actattatga cacgaaacca acaagcaagt tcaagcacct
       61 ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa acctctttat
      121 cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt ttgttgcctt
      181 tatcaatcat ttcaacgctc ctttaaatat aaaaatgatc ctatcaatct ttattttgag
      241 cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat accaagtagg
      301 aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc attttgaagt
      361 gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga ataatttgtt
      421 tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc ttttttatct
      481 gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat tctttgattt
      541 gagcatatgt tgctttgctc tccgcacttg tcaaatccag ctcaatttca acacttatgt
      601 gcttatcgac atcgagttta gacaaaagtg ctaccgtctc gacgtgatgc gtttgaggaa
      661 atagatccac cggctggact ttcttcaatt catatcccaa ttcttggtag agtttgatat
      721 cacgcgccat ggttgcgaca ttgcaggaga tataggcgat gcgatcggct cctgtttggg
      781 cgcttgcttt gataaagctt tctgtcaagc ccttgcgtgg aggatcaacc aagataacgg
      841 ttggttgaat cccatcttta agccaattct tcatagcatt ttcagctgtg tcacagacat
      901 agtgggcgtt tgaaatattg ttcagctgag catttttttt actattctca accgcttctg
      961 gaatcagttc aacaccgtag acttctttga catgcttggc gactgataaa ccaatggttc
     1021 caataccaga ataggcatca ataatcacgt gatctttttt taactctgca aagtcaatgg
     1081 ctgtttgata gagtttctcc gccatttcag tattgacttg gtaaaaggct gggccagcga
     1141 tttggaagtc atttcccaac atctggtccg taatatagtc ttgaccataa agagtgcgcc
     1201 actccttacc aaaaatcgca ttggtattct ggtcgttgat attttgcatg acagacacaa
     1261 tctctgggaa ctgcttgata acttgttcaa tcaattggtc aacacgaaaa acttttggac
     1321 gagttgtcac caaaacgacc atgatttgtc ctgaatagtg accacgacgc accacaagat
     1381 tccgaatcaa tccagactgt tccttttcgt cataaggttt taaatcaaaa cgacggagca
     1441 ggtctcgaag agctactacg acttggtcaa tgacaggatc ctggataaag aaatcttcaa
     1501 ggggcatgag gttgtgcgaa ttcttacgga aaaatcctgt ttccaagaca ccattcactc
     1561 gacgaacggg cacctgcgcc ttattgcgat acttgactgg atgttccata ccaagcgttt
     1621 cagcaacttc tacatctgca attccagcaa tcttgtagag actgtccttg acttgcttgg
     1681 ttttaaactt gagctgttct ggataagaaa ggtgtcctaa atccgcgatt cctgaacgca
     1741 ggtaagccaa atctagatct tgattacggt gtggtgactg gacaaggtat ttttcaactt
     1801 ttccaaagcc aatcttttta ttgaccttga ggacacgcat gagaattttt tcactcggta
     1861 aagcattctc tacaaaaaag accaaaccat ctaccttggc aactcctgcc ccttcatggg
     1921 tcaaatcaac aatttcaact tctacaatat catttttctt taacattctt ttctctttct
     1981 attggccgac aaaaaagacg gcaacgttac ggttaccatc cattatacca tgaaatttct
     2041 taagaaccaa aactcttgaa gaagttgaca agggcttgcc agagggagct taagaagtta
     2101 ccaccgtcct ctagcgcctt atccgcatca aagttaaggt tgatattttt aaaactgtcg
     2161 ccagcttgtg atacgatgct ttgtttaagg tcatttaggg ttttagtgaa atctgcattg
     2221 ctgaggatat cactctttga gagattcaag gcaaaattga tgatgatatt gatctggttt
     2281 cctgttatga cctgatcaag tttgtaattt tttaaggtat cttcaacaat cttgcggata
     2341 tcttcttctg tcagatttcc cttgctttct ttagctttgg cgagtcctga cttgatatca
     2401 gctagggcaa cgtttaattt attagcatca tagcctgatt tgtccttgtt ttcagcattg
     2461 atatctgaca aagcctttag ctcttcttga gccaaatctt tattggcttg cggcaccttg
     2521 gctccattag cttctagcga atagtagatc ccagccaaag cactctcacc tgtaactgga
     2581 atgggtgctg ctacagtgat tttggcgtgt tccacaccca aagttacggc tgcatttcgg
     2641 tacatatcct gagttacctt agtgatgttt tcaggtgttt caatcttgac ctcaagtggc
     2701 gatttgtcac ctagcttttg aatcttggct gatgaataca actgtaagct agagtcattg
     2761 gccacattca tgattttaga ataaacatca ggtgtcatgg tcttgagttc tttggtatct
     2821 gttgaggcat tgtagcccag ttttttaaga gtttgatttt tttggtcttc agatagggag
     2881 gaacctagga catattcagg ttggacatag gtttcatcga taactttttg aacatctgtt
     2941 gctgcatgga cgctattcat agctgttact gcccacaaga ccgcagcact agtcagaaag
     3001 agtttctttc tcatagggaa tttcctcctt tacttcttta tagtaatata tctatcttaa
     3061 agaaaactta taacaaaaac acctggtcta gccagatgtt gaaaagagag tgaaacattt
     3121 gatgatgtaa aggttaagtc gtacctgtct agaataataa tagtttcctc catttacata
     3181 gagttcagca ccgtgaaaaa tggaaatggg gtgaatataa ctataagtct ttccagtcgt
     3241 attaccaagc aagggggcaa cagtctcacg agagtactgt ttggctagag ccaaggtatt
     3301 ttccttgcca ttttgggcga taaaatcgat ataggcaggt ccaaaattat aggcttgaac
     3361 agctgtccag atatctacac ccttcttctg cgccagatag agattgcctg tcagagtttg
     3421 aatgccttgc cgaatgctag aggcattatc attgatggtg ttggtggaac cacttgcaga
     3481 ctcactagac tgcataacat ctccttcttt tccttttgtt tcagtataaa tcatagcaag
     3541 cacaagctct tcgtttgctg gggtgtcttt ttcactcaag atttctcgca ccatgggttg
     3601 ataggtcatg acttgtttga catcttgatg aacgcggtaa gctttatagc cagcaaaaag
     3661 gaagactgct agtacaagca ctcttcgaat tcgtttaaac attatttact ttggatatcc
     3721 tcgatatttt tgattaagat agagtaggtt ccattttcgt tttggataaa ctcaacagac
     3781 tcggcgtctt gatagacgtt attgggaacg atgagctcaa ttccatttga taaggagagt
     3841 ttttggtttt caaatttctt taattggcga ctggcatcaa tttcatcaaa ttgaacaggt
     3901 tctggtacgg cttctttgac ttggtcaata aagctcaaac gagccgtcag attgttgtca
     3961 aaaaggtcat tagccaattt ctcaggtgac aattcattgc tttcttctag gttgttgaaa
     4021 atcgctgatt tgaccttgga ttgaaattga aaatcatctg tgttaaaaga ttcagcaatt
     4081 ctctgggctg ttttttccag ttccttgata gattttttag gagaaatctt aggagcgaca
     4141 gcaagaagat tatctgaaaa atagttcaaa aaagtcccgt tgtacttgat tcgtttttca
     4201 atcaggtgat acttgctact ctgaagattg accaccaagg cctcatcagc tcctgttcca
     4261 aatccaggca ggttattctg agttagcttg attggattat caacttctcc tccgaggtgg
     4321 gtcaaggtct cccgcagggc aattcgcaag aaagcgaaat gttctacacc ttctttagaa
     4381 aattgaacaa aaatcaagtc attggtcttg agattttcag aaatgctaaa ctcctctttc
     4441 cagagattag ccagcgttac tgatgtctcc aacaaatcgt ctgtaatatg attgaagaag
     4501 ggattttctt cttcgaaaat cccagtcttg gcttcatctg aatacacatg ttcaattttt
     4561 ttacgtaggt attcttcgat ttttggagta atattgagaa acttatctgc taagaacagc
     4621 tcggtatcat ccggactgaa ctggtgaata atggctttct taatataaat gtccataaaa
     4681 gttttattcc tcgtataatg ggaaggcatc tgtcaattct ttgactgcac ttctcacttc
     4741 ttctaataca gcctcatttt ctgaattctt aagggtttta atgatgagtt cagccacttt
     4801 gcgactttct tcttcaccaa atccacgtgc agtaatagct gctgctccga tacgaatccc
     4861 acttgtcttg aatggtgaca agctttcgta agggattgag tttttattta aggtaatatt
     4921 gacttcatcc aacaagtttt gagcaacttt gccgttttct acaactttag tcacatcaac
     4981 aaggaagaga tggttttcag ttccacctga aataatacgg aaatcagggt cttgcaagaa
     5041 gacatctgcc atagccttgc tgttcttaat tacattggca gcatattcct tgaaggctgg
     5101 atccaaaact tctttgaagg aaactgcctt agccgccaca acatgctcta aaggaccgcc
     5161 ctgaatacct gggaaaatag ctgaattgat ttttttagca agttcttcgt cattggtcaa
     5221 aatcaaacca ccacgaggtc cacgaagggt tttgtgggtc gttgttgttg tgatatgagc
     5281 gtatggaact gggcttggat gaaggccagc cgcaaccaaa ccagcgatat gggccatgtc
     5341 caccatgagc ttcgcaccga cagcatctgc gatttcacgg aattttgaaa aatcgataat
     5401 ttgagaatag gctgaagcac cagctacaat cagttttggt tttacttctt gggcttgttt
     5461 caagatagca tcaaagtcta agagttccgt ttcaggatca acactataag aaacaaagtt
     5521 gtaggtttga ccagagaagc taacaggagc cccatgagtc aaatgaccac ctgatgccaa
     5581 atccattccc ataaccgtat cacctggctc aatcaaggac atgtaagccg cacagttggc
     5641 ctgacttcct gaatgtggtt ggacattggc aaatttagca ccgaaaattt cttttgcgcg
     5701 ttcaatagca agagtctcta caacgtctac tacatcagtt ccaccataat aacggcgtcc
     5761 tgggtaaccc tcggcatatt tatttgtcaa gatagaccct tgagctgcca taacagcctt
     5821 ggaaactacg ttttccgaag caattaactc gatattattt tgttggcgtt cttcttcttt
     5881 ggcaatagca ttccagagat cagcatcata tgctttaaaa tcatctttgt caaaaatcat
     5941 aggtcttctc ctttattgtg tgactagtcc attagtttga ttttacaata agaaaatcaa
     6001 actaaaagat gcgaataaac cgtttctgca ttttatcaca agtatagcca actttttcat
     6061 aaaatgcatg agcacccaga cgatgattgg cagaatttaa gcggataaac ccataaccac
     6121 atcttttggc ttcttgttcc aacccttgta gtaaactttt accgatacct tgaccttgcg
     6181 cttgaggtga aactgctaaa gctaagatat taaatcctgc tttggaatag agtgattcgt
     6241 aaacttcagc gtggacatat ccaagtaaga catgattagc tgcatcctca tagccaagta
     6301 ggaaatgatg ggaatcctga gacagtctag ctagttggct agccgtttcc tctggactaa
     6361 aagtataacc caaagcctct tggttgatgt cacatatcgc tttcacatct gtttcttgca
     6421 aatctcttag catttcaagc ctcctcaaaa gaaatctctg gcaaccgagc aagaatatct
     6481 tctcgtttaa ttgcgccttg gcgtaagatt ttcaccttgt ctccagacaa atccacaata
     6541 gttgaatcct gtccagttag aaaagcatcg tcttccagac ccagaacctc ttggtcaaaa
     6601 tccttcagaa tttgttcaaa ggttacacca cttgcctgac ctgagatatt ggcagacggc
     6661 ccaatcaagg gacctgtctc tcgaattaaa tccagtgtga tagggtgact gggcatccga
     6721 aatccaatag ttgcaaggtc agaatttacc caatagggaa ctcggtcatt ggcttcgaga
     6781 ataatggtca agggacctgg caaaaaggtc tctacaagtt tttgtagata agctggctga
     6841 ttctttgaaa agtgcaagat gtcctcgaaa gaggcgatat tgagattgag tgccttatct
     6901 ctaggacgac gtttgagttg gtaaacatgg tcaactgctt tttcatctaa ggccttggaa
     6961 aaaagaccat aaacagtctc tgtaggtaga acgacagctc caccattttc caactcttgt
     7021 ctaatcctgt ccatcatcaa ccacaaccat cctattttga ccaaattggt ccttgagtgt
     7081 tcgaactctt ttttcaggaa gatgtttcct aaaaagttcg ggaacacttt gaccttgctt
     7141 gtatccaatt tcaaggtaaa tcttaccacc atctttgaga tagtcttttg catcttccgc
     7201 aattctacag taaatagcta ggccatcctc gtctgcaaag agagctagat gaggctccga
     7261 atgcaaaaca ttcaagccta cctctgactc atcttcacga gagatatagg gtggattgga
     7321 tacaattata tcatattttt cagaaatttc tgcaaaacaa tcagattttt taaaaaatat
     7381 attaagattt tgattgctag cattctctga tgcaagctct aaggcctctt gagaaacatc
     7441 tgctgctgtc actgaccaat ctggtctatt ttttgctaga gcgagagcaa tagctccact
     7501 acctgttcca atatccagaa ctgagagatt cgtctcaaga ttttcagtta gaataagctc
     7561 cactaactcc tctgtttctg gacgaggaat caaaacccgt tcatccacct ttaaatgcat
     7621 tccataaaaa tctgcctgtc caatgatgta ctgagctggc ttgtgagctg ctagttgctg
     7681 gtaaatatct tctacaaatt gtttttcttc ctctgttgtc acctcctgct ggagggcaaa
     7741 aataaagtct gtaaaagata gatttttcag actacgatag acaaaagaga ggctttccgc
     7801 ttcctctcct tgtcttatca actcttcttc aaaatttgaa aataattgag ctaatttcat
     7861 tatttgttta attcttctag tttttgtgtt tggtcataaa gcaccaaggc atccacaact
     7921 tcgtccaatt taccagacaa aatcgtatct agtttttgga gggtcaagcc gatacggtgg
     7981 tctgtgacac ggttttgtgg gaagttataa gttcggatcc gttctgaacg gtcaccagta
     8041 ccgattgtcg acttacgctc agcgtcctgc tcatcttgag caatctgagc aaagtggtca
     8101 gcaacacggg cacggatgat tttcatggcc ttctcacggt tcttctgctg ggtacgttct
     8161 tcctgcatct caaccttgat attggttggc aagtgaacga tacgaacggc agtcgcaacc
     8221 ttattgacgt tctgtccacc agcaccagag gcgtgataga tgtcgacacg aaggtctttt
     8281 ggatcaatgt cgtattcaac ctcttcaact tctggcataa caagaactgt cgctgtcgaa
     8341 gtatgaacac ggccttggct ttctgtcaca ggaacacgtt gcacacggtg ggcacctgat
     8401 tcatacttaa gcttagagta tacagactga cctgaaacca tagcaaccac ttctttaaaa
     8461 ccaccgacac cattcataga ggcttccatg acttcaaagc gccaaccttg ggcttccgca
     8521 tacttttggt acatagttag caaatctcca gcgaaaagtg ccgcttcgtc tccaccagct
     8581 gctccacgga tttcaaggat gatattcttg tcatcgtttg gatcctttgg aaggagcaaa
     8641 attttcagtt tttcttcata ttcttctttt tcagccttgg catctttgag ttcttgcttg
     8701 gccatttctt ccaagtccgc atctccgcct gattccttaa tcatctcttc ggcatcgacg
     8761 atattttgaa ggacttgttt atactcacgg taggctatta cggtgtcacg attggaagct
     8821 tcttcttttg aaagctccat aaaacgcttg gtgtctgaaa cgacatcagg gtcactcagc
     8881 aattctccta gttcttcata acggtcttct acagcttgta gttgatcata gatgttcatt
     8941 ttttctcctt atttctcaat tgttaaatca tagattgcta ctacttcatt ctcggatatt
     9001 tccccagttt ctttaaatcc ataactgaga taacaaaatc ttgcctgttc attttctggt
     9061 tcataagaca accaaagttt attgcttaaa cctgctggcg ctgttcgaac atagtctagt
     9121 actttatcca taattggttt aaaatatcct tgattttgaa aattcttatc aatcataaaa
     9181 cgaaatagta aataatttcc actactaatt ccgatctttt tatcataagc tatcatcaca
     9241 aaacctataa ttacatcatt atcataaact gccaatggag ctacaaaatc tccattttta
     9301 gtgtagacgt atgcttcagc taaactaatt gcgttggttg caatgaattg tttttgatat
     9361 tccttgacat ccaaatttaa aacatcaaaa taattttcca ttgtaacatc tcttagttca
     9421 attgtcatag ttttgctcct tgttagaggt tatcattggc gcaaaataat gtttacggca
     9481 aaccgagata taggtttcat taccaccaat ctggatctgt tctccatcgt aaacgggcag
     9541 tccatcctgt gttcgcaaca ccatggtcgc ctttttcttg caatactgac agatggtctt
     9601 gatttcgtca atcttgtctg ctaaaagcaa gagatatttg gaaccttcga acaattcatt
     9661 gcgaaagtca tttttcaagc caaaagccat gacgggtatg tctaactcgt ccacaacacg
     9721 agctaggtca taaacatggt ggcgtttgag aaactgggct tcatcgacca aaacacagta
     9781 aggtttttct ggtaggtctc ggatatagcc aaagatatcc gttgtttcct caatcgcaag
     9841 ggcagggcgt ttcatgccaa ttcgactcga cacatagcca acaccgtcac gcgtatccag
     9901 agccgaggtc ataatcacaa caccttttcc ttgctcctcg tagttatagg ccactttgag
     9961 aatctcaatc gttttaccag agttcatggt cccataacga tagtacaact gtgccatgtt
    10021 tcttgcttca cgtccatttc taaatttttg ctacattcta gtatatcata attttcttaa
    10081 gctttaaacg gcaaaatgtg gtaaaatgga agaaatcaaa actagtggag gaagctatta
    10141 tgccatttgt acgcatcgat ttatttgaag gacgcacgct cgagcaaaag aaagctcttg
    10201 ctaaggaagt aacggaagca gttgtccgca acactggagc acctcaatct gctgtccatg
    10261 tcatcatcaa cgacatgcca gaaggaactt acttcccaca aggggaaatg cgtactaaat
    10321 aagctagctt aagcagaatt gcttaggctt tttcaatctc caagtagcac ccattgaaga
    10381 aatagtctaa atttgttaca atttgaaaag agacttggaa aattccaaga aaagagcaat
    10441 taattaaagg aaacattatg attacacgtg aatttgatac catcgctgct atctctactc
    10501 cactaggtga aggagctatt ggtattgtcc gcctgagcgg aacagacagt tttgctattg
    10561 cgcaaaagat ttttaaagga aaagacttga gtcaggttgc tagccacact ctcaactacg
    10621 gtcacattat tgatcctctg actggtaaag tcatggacga ggttatggtt ggggctatga
    10681 agtctccaaa gaccttcact cgtgaggata ttatcgagat taacacccac ggtggtattg
    10741 cggtgaccaa tgagattctc cagctagcta tccgtgaagg agctcggttg gcagaacctg
    10801 gtgaatttac caaacgtgcc tttttaaacg gtcgcgtaga tttgacacag gccgaggcgg
    10861 tgatggatat catccgagcc aagacagaca aggccatgaa catcgcggtc aaacaacttg
    10921 atggttccct ttctgatctt atcaataata tccgtcaaga aatccttaac acacttgccc
    10981 aagttgaggt caatatcgac tatcctgagt atgacgatat tgaggaagcc actactgctg
    11041 ttgtccgaga gaagacaatg gagtttgagc aattactaac caaactcctt aggacagcac
    11101 gtcgtggtaa aatccttcgt gaaggaattt caacggctat cattggacgt cccaacgttg
    11161 ggaaatcaag ccttctcaac aacctcttgc gtgaggacaa ggctatcgta acagatatcg
    11221 ctgggacaac acgagatgtc atcgaagagt acgtcaacat caatggtgta cctctcaaat
    11281 tgattgatac agccggtatt cgtgaaacag atgacatagt ggaacaaatc ggtgttgagc
    11341 gttcgaaaaa agccctcaag gaagccgact tggttctact agtgctaaat gccagtgaac
    11401 cactgactgc gcaagacaga caacttcttg aaattagcca agataccaat cgcattattc
    11461 tacttaataa aaccgacctg ccagaaacga ttgaaacttc gaaactacct gaagacgtta
    11521 tccgtatttc agtccttaaa aaccaaaaca tcgacaagat tgaagagcga atcaacaacc
    11581 tcttctttga aaatgctggc ttggtcgagc aagatgctac ttacttgtca aacgcccgtc
    11641 acatttccct gattgaaaaa gcagttgaaa gcctacaagc cgttaatcaa ggtcttgagc
    11701 tggggatgcc agttgatttg cttcaagttg acttgactcg tacttgggaa atcctcggag
    11761 aaatcactgg ggatgctgct ccagatgaac tcatcaccca actctttagc caattctgtt
    11821 taggaaaata agaaaaatcc atgatccttc attcggtcat ggattttatt gtctttatta
    11881 gtaatctggt cttaagaccc ctgttacagt tgccttagtt gcttcgtagt cgccatctac
    11941 gacaaccttg ataatgcgtt tggcatcttc ttctggtgct ggaacaagag gtagacgagt
    12001 gggtccagct tcaaatccca tatagttaag aattgcctta actggagcag gacttggata
    12061 agagaagaga gcattaacct taggaatgaa tttacgctga attgctgcgg ctttcttcat
    12121 atcgctttct gcaatggcag taaacatctc gtgcatttca tccccatttg tatgagaggc
    12181 aacagaaata accccatccg ccccaaggtt catggcatgg aaagcatctc catcctcacc
    12241 tgtataaatc aagaactctt caggcttgtg ctcaatcaag taagccatat tagccaagct
    12301 agtacattct ttgacaccga taatatttgg atggtcagcc aagcgaagca tggtttctgg
    12361 agtcaattcg acaactacac gccctggaat gttatagata ataattggta ggtcagaagc
    12421 atctgcaata gccttaaagt gctgatacat cccttcttga gaaggtttgt tgtagtaagg
    12481 aacaatagca agcccagctg cgaaaccacc aaattccgct acttctttga caaactcaat
    12541 agagtcacgc gtatcattag tacctacacc cgcaatcaaa ggaacgcgtc cattgacaac
    12601 cttttgtaca gccgcaaaca actccaactc ctcatcgtgg gtcaaagttg gactctcagc
    12661 agtcgttcct gcgagaagaa ttccatccgt atgatgggcc aataaatgct caatcaaggc
    12721 tggaatagca tcaaagttaa tagaaccatc ctcatgaaag ggggtaataa aggctgtaat
    12781 gattttacat tcttttaaat cttgataaga catgaaaaca cctctctatt tcaaagaagg
    12841 agttcatctg aactcctaaa cgatatgact attttaattc aaatttcaat tcagctgttg
    12901 gacgaaccaa tccacgttca tgaagagttt cagcaatctg aactgagttc caagcagcac
    12961 ctttgagaag gttatctgaa acaacccaca tgtgaattcc tttttctgca tccaagtctt
    13021 tacggatacg accaacaaag gtatcacgtg aaccaactgc attgatggct tgaggataga
    13081 tttgatgaac tacatcatct tcaagaacag cacctgggaa ggatgcgata gctgctttga
    13141 cttcttcaat tggagccact tcctttgttt caatataaac tgactcagag tgagctgaca
    13201 agactggaat acgcacacat gtcgcagata ctgcaatgct atcatcttcc ataattttct
    13261 tagtttcctt ggtcatcttc atctcttcgt acgtgtaatc attatcagtg aagacatcaa
    13321 tttgtggaag agcgttaaag gcgataggat aatgtttctt gtcaccacct gaaggcaaga
    13381 tttccgcatg caaatcacgt ggtttcactc catcattcaa gacctcacga agttcacgtt
    13441 gtgtctcaag aattgctccc ataccagcac ctgaaacggc ttgataagtt gaaacaatga
    13501 tacggtccaa gccccatttt tggcgaaccg gctcaagagc caccatcatt tggattgttg
    13561 aacagttagg gcaggcaatg attccgttgt gggcatcaag tgcatgagca ttgacctctg
    13621 gaacaaccaa aggaacatct ggattttgac ggaaataaga tgtattatcc actactacca
    13681 cgccagcttt tactgcgtat ggtgcatact tagctgatgt cgaaccacct gctgaaaaga
    13741 gagcaatatc aactccttca aaagctgttt cagtcgtttc ttcaatcgta atatcttgat
    13801 ctttaaattt caatgactta cctgctgaac gtgcagaagc aaggtaacgg attttatcga
    13861 ttggaagtgt tgattcttcc aacattttta tcatctgagc accgacagca cctgtcgcgc
    13921 cgactacagc aactgtatat cccataaata acctctttcg gaattttcta aaaatttcta
    13981 taatagaagt attatactac tttttccatg aattgcaaag ctttttcatc attttttgga
    14041 aaataaaaat ccccaatcgc tagactaggg attaagaggc atcttcatgt gatgagcagg
    14101 ttcacacaac tcatcaaggt ccgctcctgc gttatgacct ccttatgctc aatagtagcc
    14161 cgaagactac ctatcgactc atcgcatcta ctattctact aaagagaatg acttttgtca
    14221 acagacgaaa ctctttatac tcttcgaaaa tctcttcaaa cttcgtcagc gtcgccttgc
    14281 cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaaaca tgttttgagc
    14341 tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt
    14401 tcctagttta ctctttgatt ttcattgagt attagtttta tttacacagg tgcataagaa
    14461 atatattgga aaaattgggg gcagacataa caaaaaccct tgtaaatcaa gggcttttct
    14521 gtctatttaa tgctaattgc cgggattgaa ccggcgacct catccttacc atggatgcgc
    14581 tctgccaact gagctaaatc agcttactta agaagtatac tataatcaga aaaacttgtc
    14641 aagtttcctt agaaattttc cataggaaaa agccaggtaa gaactaccta gcttatcttc
    14701 ttctatttac ttaaatcaat tcgacgacca attttaccaa taataatcgc tccaataatc
    14761 aatgaagcaa attcaccgat tcctgttgta aaccaagtaa ggaagaatgg taattctgct
    14821 acaatattta actctgcagc gatagtaaac atggaaattg aaaagaggat tgaaaagaag
    14881 aaatgatctt ttcgaatcaa tccgttaaag aggtaatcct tgctatattt tgcaaaaagc
    14941 caaacaccta aactgaggaa tactaaggtt gaaccaccac caacaaagac atctagcagt
    15001 ccgaagctaa agaaattagc aatcatacaa ccgatggtaa ctccgatgat gtacttagga
    15061 ttgtaaaaag ccataaagtt catcatttct gaaatacgaa actgataagc accatagctg
    15121 atggcattta ggggcggtgt gacagtcaaa acgacgtaga tagcagcgac gattgcaata
    15181 tctgcaacat cacgaatagt taattttttc atcttttctc ctttggcggt tatccgcgtg
    15241 taatatgctt ggtgaaagaa gctaagcacc aagggttgat tcattcaacc ttactagtat
    15301 agcacaatag cctgctttat gctatactta aagcatgaaa aaacctattc aaaaattttt
    15361 taacaatgaa atcttagcct atctcttttt tggccttgcg acaactctag tttcgattct
    15421 ctcacgacta gtcatttatc aactaagtca tcaggaactc cttgctacgg ccttggcaaa
    15481 tattatcggt atcttatttg cctttatcac aaatgatacg attgtattta agcaaaaaaa
    15541 gaaaaataga cttatccgtt tagtgaaatt ttctcttgct cgcctttcta cttttctgtt
    15601 agacttgttt ttaacttttc tctttgtaac ccaatttcct cacatcatag ggcaattcgt
    15661 aactgaaaac attgataaga taaatgctat tgaaacaata attgcacaaa tattcattat
    15721 tatcatcaat tatatcttga gcaaggtcta tatttttaga aaataatgct ccatttatga
    15781 gccttttaat tgccttctat aatgatttag gatacaatga aacacgaaac attgaaagga
    15841 aaacagtatg ttaaaaaatc taaaatcgtt cttgcttcga ggaaatgtta ttgaccttgc
    15901 tgtcggtgtt gtcattgcct ctgcttttgg tgctatcgtt acttcacttg taaacgacat
    15961 tatcactcct cttattttaa atcccgcttt gaaagctgct aaagttgaac gtatcgctca
    16021 actttcttgg catggagtcg gctatggtaa cttcttaagt gctattatca attttatctt
    16081 tgtgggtacc gccctcttct ttattatcaa gggcattgaa aaagcacaga aactgactgg
    16141 cataaaggaa gaaaaaactg ccgaaaaaaa accaaccgaa ttggaagtcc ttcaagaaat
    16201 aaaagctctc cttgagaaaa aataatcgat taaaaggatc tagcgcaaag ctaaatcctt
    16261 ttttctttac tctttgggta acttgcctgc tttttcaagc atttttttga taagataagg
    16321 catcttaaca ttctcccgcc cttttttctc aatcagtttt ttcacaaatt ctggcatttg
    16381 taaatcttca gattcagcca aaatgttttt cgtctcgtct gaaggaacta actcatcaaa
    16441 ggtctcttct tctgggagaa attccttaaa ctcttgattt tcattgattc taattgtatt
    16501 gaccaaagca tcaatcaaac gagaatctgt atttggcatt ggtggacgat ggtagtttac
    16561 cccaaattcc tgacataagt cataacattc cacatcgttg tcaaacaaga cctcaatatg
    16621 ctcactgatg aaactaatag gtacaaaaat ataatggtct ggatgttctg tctgttctct
    16681 gagatactcc aaaacatctg gtttaatcca tggaatacca atatcactct cactctgcca
    16741 agtgttggta tattgttttg aactcaagcc cagtttttca gcgactagct tgctattttc
    16801 gaaaatttgg tctatataag gatcaccaaa gtccaaggca aaaataggca cactatgggc
    16861 tgaaaagatg actttaaagc tgtcttgctt tacttcttct tttaaaatct tagcaatttc
    16921 atctgcccaa tagtttaaga gcgcttcttc ctgataccag tccttaatga ccaaaaattg
    16981 aatttgtttg ctttctaaaa atttttcata gcccatgaca gagtagaaag aataatgggg
    17041 ctccaaaatc aagcaaatac actgttcaat tccgtctgct tccatttgac cgatcacatc
    17101 tggaataaag ggactggaaa acttattagc aaagtagaca ctgtattcat tccccagcct
    17161 agcttctacc aaggcaacct cttgacgggt aattttttgc agaggcgtgc ctccaattcg
    17221 tacataatta tcatagagag tctgaatctc gtggtcttgg ggtctcaccc cacgacgaat
    17281 atttgtgaaa aaatcagcta caccttcaaa ggtaatctct tctggtgaac cgaaggtcat
    17341 cattaaaatt gcttttttca tgactgcttc cttgtgatgt ttttatcaag tttattttat
    17401 cattaattca tcgataagta aacgctaaca tagggacatt ctcaatcttc tgtcttttgt
    17461 ttttctcttc tttctatgat acaatggaaa aaataaattc aaaaggagtt tttttatgac
    17521 ttatccaaat ctcttggacc gcttcttaac ctacgttaag gtcaacacgc gctctgatga
    17581 acactctact actactccaa gtacacagag tcaggttgac ttcgcaacaa atgtcctaat
    17641 tcctgaaatg aaacgtgttg gactgcaaaa tgtttactat ctaccgaatg gttttgctat
    17701 tggaaccttg ccagccaacg atccgtcttt aacacgtaag attggtttta tatcgcacat
    17761 ggatactgct gattttaatg ctgaaggagt caatccacag gtaattgaaa actacgatgg
    17821 tggtgtgatt gaactaggga attctggttt caaactcgat ccagctgact tcaagagtct
    17881 tgaaaaatat ccaggacaaa cgctcatcac aacagatgga acaaccttgc taggtgctga
    17941 tgacaagtca ggaattgctg aaattatgac agccattgaa tatctaactg ctcatcctga
    18001 aattaagcac tgtgagattc gtgttggttt tggtccagat gaagaaatcg gtgttggtgc
    18061 caataaattt gatgcagaag attttgatgt ggattttgcc tacactgttg atggtggtcc
    18121 actaggtgaa cttcagtacg agactttctc agccgctggt gctgaattgc atttccaagg
    18181 ccgtaatgtc caccctggta ctgccaaagg gcagatggtc aatgcccttc agctagcaat
    18241 tgattttcat aatcaacttc cagaaaatga ccgacctgag ttaactgaag gttaccaagg
    18301 tttttaccat ctaatggatg tgacaggtag tgttgaggag gcgcgtgcaa gctacatcat
    18361 tcgtgatttt gaaaaagatg cctttgaagc gcgtaaagca tccatgcaat ctatcgctga
    18421 taagatgaat gaagaacttg ggagcaaccg tgtcactctc aacttgacag accagtacta
    18481 caatatgaaa gaagtcattg aaaaagatat gactccaatt accattgcta aagccgttat
    18541 ggaagatcta ggtatcacgc ctattatcga accaatccga ggtggaacag acggctctaa
    18601 gatttccttt atgggaatcc caactccgaa tatctttgca ggtggcgaaa atatgcacgg
    18661 acgttttgaa tacgttagcc ttcagactat ggaacgtgca gttgatacca tcattggcat
    18721 cgtagcttat aaaggctaaa aagacgaggt agctcagcta cttcgccttt ctttttattc
    18781 tactggtttt tcttgatttc cagtagttgt agaagattct gttgtttcat tttctgaagt
    18841 tgattcagca ggtttagaat ctcttgtatt gcttggtttg ttttcgtcgc tagcagtttc
    18901 aatgttagat tctgcagttg cgtttggttg gttctcagca ctggtgttat cactatttgc
    18961 ttcagcattt cttgctggac ttgtttcttc acttgcgcta gcttttgact ggatttgatg
    19021 attcaaaact agaatagctt ttgtcgattc aagtaaagct gttttgtctt tactcttagc
    19081 agaaagttga tctaataatg catccacctt atcaaagtcc gcatcagatc cattattact
    19141 ttctaaataa gagtgaagcg acatgagaat atcgtagagt ttttgataga gtacaagtgt
    19201 ctgagaatct tgctcagcat tttccttttc ttgttgaagg gcgctagcga tacgagtcaa
    19261 aacatctttt acctgactgt ttacttcatc caagtctgca tcagccttgt ttgtggcagc
    19321 ttttagattt tctacttctt ctgccaagga ttgtctgatt ccttcttcat ggatttgttc
    19381 caagagttga tttgccttgc tcaaaagact ttctacttct tccttgctat ctgtcgcaga
    19441 ttattggttg ctatctacca tgtactccta aaacaggaga gttataatcc aagattacaa
    19501 ggccttacag aaataagaaa tccagataag acaatgttcg tccaagacgc tattcgcttc
    19561 gcacaacagc acggattcaa tatgctttaa ttttaaagtt taggtgtcaa gacctctttt
    19621 tagtgtgccc aaaatttaga gaagtaatca atcaactaac ttttattttt ttcaaacttt
    19681 cagtaaactg acctaaagct aactcaatct gtctttgtag atgcttctgc tatcagctag
    19741 aagttgatct acttttgcca agactgcctt ctcatcaaaa gttccaggtt gatagttgga
    19801 ttgcagggat ggaatcttgt ttttcaaagc cgcttcatat cccttagttt gaaccttgat
    19861 gtagtgattg tggtcgccat gaggaatcac aaaaccttct gaatcttcac ttataattcg
    19921 attggcatca aaaccatgac catcttcttc ctcatggtgg acatgtagtg acggattact
    19981 taatacagaa ctagaagaac ttcctgcctc ttccgtgtta gagtgtgatg ggggattgtt
    20041 aagagatgac ttaggaatat agtgatagtg atccccatgt cttactatat aagcatcacc
    20101 tgtatctctg acaatatcat tagggttaaa gacatatgtg gctgctaatt cacctgccga
    20161 caagtcactc tcaggaatga aatgatagtg accaccatgt ggtactatag tagattgaaa
    20221 tagaatatga gcaaattgat aaggggattt taaagtaatt tctaacaatg atttagaaac
    20281 tatgatgtgc tattctaaat tcaactcact atatataacc atcatcggta gtataacgtc
    20341 cctgtaattt tgctacagat acttctgcac tagctccttt atcctcttta ccatgttctt
    20401 gtttttggcg attgatttca tcttttgttc gtacattttc tgcatgagct tgatctttaa
    20461 ggtaaacata atactttcca tctaccttaa tactatatcc tcccttaacc taactgacga
    20521 tatcttgatc tttcggctga tagttggggg ctttcattaa tggctcttca ctaaagagcg
    20581 catcaaaagg aactttacca ttatagcagt gataatgatc gccatgagaa gttacataac
    20641 cttgacctgt aatcttaata acaatttgtt ttgcttgaat tccttctttt tgactaacct
    20701 agtctggagt caaattttca gtcttcttag tgtctttatt actgtttaca tatgaaacac
    20761 gatttttatc tgtattggtc tgttagctat gttggttcag agcataaaca cacagactta
    20821 aggaaaggat aacagcagat ccagctgcta tatatttc
//
LOCUS       DAVMAF010000030        20282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMAF010000030 DAVMAF010000000
VERSION     DAVMAF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.030"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            210..392
                     /locus_tag="VYO97_001144"
     CDS             210..392
                     /locus_tag="VYO97_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1753948.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            337..1043
                     /locus_tag="VYO97_001145"
                     /pseudo
     CDS             337..1043
                     /locus_tag="VYO97_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1113..1475)
                     /locus_tag="VYO97_001146"
     CDS             complement(1113..1475)
                     /locus_tag="VYO97_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753949.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1765..3645)
                     /locus_tag="VYO97_001147"
     CDS             complement(1765..3645)
                     /locus_tag="VYO97_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1753950.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWSVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLQTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDHVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPNNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTNFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3639..4508)
                     /locus_tag="VYO97_001148"
     CDS             complement(3639..4508)
                     /locus_tag="VYO97_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1753951.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGVSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAATGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4592..7237)
                     /locus_tag="VYO97_001149"
     CDS             complement(4592..7237)
                     /locus_tag="VYO97_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1753952.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7328..8608)
                     /locus_tag="VYO97_001150"
     CDS             complement(7328..8608)
                     /locus_tag="VYO97_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1753953.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKNFLLDQLVACDGGTGLMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8779..10863
                     /locus_tag="VYO97_001151"
     CDS             8779..10863
                     /locus_tag="VYO97_001151"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1753954.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDMATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTSFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKILVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10903..12582)
                     /locus_tag="VYO97_001152"
     CDS             complement(10903..12582)
                     /locus_tag="VYO97_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1753955.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13337..14566
                     /gene="arcA"
                     /locus_tag="VYO97_001153"
     CDS             13337..14566
                     /gene="arcA"
                     /locus_tag="VYO97_001153"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1753956.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPVYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14624..15640
                     /gene="argF"
                     /locus_tag="VYO97_001154"
     CDS             14624..15640
                     /gene="argF"
                     /locus_tag="VYO97_001154"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1753957.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15805..16752
                     /gene="arcC"
                     /locus_tag="VYO97_001155"
     CDS             15805..16752
                     /gene="arcC"
                     /locus_tag="VYO97_001155"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1753958.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            16961..18472
                     /locus_tag="VYO97_001156"
     CDS             16961..18472
                     /locus_tag="VYO97_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1753959.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18494..19825
                     /locus_tag="VYO97_001157"
     CDS             18494..19825
                     /locus_tag="VYO97_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1753960.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5789 a         4528 c         4141 g         5824 t
ORIGIN      
        1 ctctccataa acacagtata tcatttttaa attttaagtc aaggaaaatt aacccacttt
       61 aatttctttt ttaacccgtg ttaaaaaaga aattaaagtg ggttaaaaaa gaaattaact
      121 gagtaaaaac tctcgaaatt caattcgcta tattatcact caacttgaac aaataccaga
      181 accacaaaaa acagatgcaa aggaaaaata tggacttaaa atttgaaggg gttgatttag
      241 aatacaaaaa agctaaaaac aatcttccag agtctttctg ggaaacctac tcagcatttg
      301 caaatacaaa tggcgggaaa ataattctcg ggatagatga aaaaaacatt gacacctacc
      361 aaagagtcaa tagactccct gcgaaattat gaagcaagta aacaattaac tgatacacga
      421 tttaagcgtc ttgttggtgt tcagcgcacg acttttaaag agatgttagc tgtattaaaa
      481 acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac
      541 cttcttatgg ccactcttca atatgtgcga gaatatcgaa cttatgaaca aattgcggct
      601 gattttggta ttcacgaaag caacttaatc cgtcggagcc aatgggttga agtaactctt
      661 gttcaaagtg gtgttacgat ttcaagaact cctctcagtt ctgaggacac ggtaatgatt
      721 gatgcgacgg aagtaaaaat caatcgccct aaaaaaagaa ttagcgaatg attctggtaa
      781 aaagaaattt cacgctatga aggctcatga agatatatcc tcaagcacaa actccacgta
      841 aatccagcaa actcaagccg ctaacagctg aagataaagc ctgtaaccat gcgctatcta
      901 aggagagaag caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa
      961 caacctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggtatta
     1021 tcaatcatga actaggattc tagttttgca ggaagtctat tgaaatacta agcctacaca
     1081 gactgattcc aattgttttt tagatttcaa ttttaccatt cttcatagtc aaaaccatca
     1141 taatctggat attgttctgg cgacgttcca accgaacgta cgagataagc ttcttcgtcc
     1201 tctgtctcga tttctctcag tacttggcat tcaaaacgcc attcatcacc gaagtcgaaa
     1261 ataaacttaa atttttgttt gacacttagg ctatccagat agacattttc tgtgtaacgt
     1321 tcttcaacat cgtcactaac aaagctaaga aagtaagaat ctgcatgact ccactcaaca
     1381 ttatccatga aaaatgcgtg agcatgatca tttgcaaaat cgaaactcca taaaatcaca
     1441 tctgctagtt ccgctaacgt ttttgttttt ggaatctata tatgtcggta gcaacctttt
     1501 ttaatttgac ggaaatgatc caagcttcta cagatgctgg cttttcagct tctatttctt
     1561 gttttactaa ttttaactgt agcatttgaa accagataat tcttctgttt cacgcgaaac
     1621 accctgcgcc aagcaaggtg ttcttgtttt tatactcaat gaaaatcaaa gagcaaacta
     1681 ggaagctagc tgcaggctgc tcaaaacact gttttgaggt tgtagatgga agctgacgtg
     1741 gtttgaagag attttcgaag agtattaagt cgtataaatc gtcgaagctg tcgcaatggt
     1801 atgccactgg ttagctgtag ttgctgcgaa atccttgtct gcgtagaagt tagtaaatgg
     1861 taggatttca acttccagct cgtcgatgcg gtcaagctgg ccagccagat aaacctcgat
     1921 acggctttct gctcgtttga tgcgttgcaa gagtccgccc atacggatgt caactgtatc
     1981 caaaccaaag accttgtttt ctttcagcca ttggtgacta aagagggcat ggaagtcttc
     2041 aatttggctt ctaagttctg gtaattcttg tctggcgatt tgttgtaaac tttctttatc
     2101 atccgcttgg taggcctgac gaatgcgtcg tcccacatct actttgctac ttaaaatagc
     2161 attcaactgg gcctgagttt caaagagata agcatagttt ccagcttttt ctttaatgtt
     2221 agcaagcgtc tcagcagcct gagcgaagtg cggtttgtcc tgttcaggtg tcatgtgttg
     2281 atcaagaatc ggacaaagaa catcctgata aaaaacatag cggttgggat tgataccgct
     2341 gagattgcct ggtaggtctg gtaagaggtt ggcaaggtca atctgcataa aatcttcaac
     2401 cgttagacca gtattggtct tgaaatgcgc agacaaacta tctaggtcat tgcgatagct
     2461 gagttctgcc cagatttgca agcttggtag gatagagaac tgggcagttt caccaccatt
     2521 gtctccccaa cccgttacga tgacttcttt aatctgattg gcacggcagg ctttattggc
     2581 ctcgatagcc actagacggc tgaaatggtt attgggtgtg aaaccaatcc acttccaagc
     2641 tccccctgca aatgcaaggt catggctaat cttgtgatgg ttgcggaaat tacggttgta
     2701 tttttcctcg ctatcctgat aataatccca gtaaaccaga gtcacatggt ctttgagacg
     2761 gtctaggtag acacgagttt cttctggaat ttccacatca cggtcgtact ggccatccgc
     2821 tgacatgagt ttgaagaaca tatcactcca catctggcag tggaaaccat atttgtcagc
     2881 aatatccagc acgcgctcca agtgttggca catgaggaga ctacgatcca caacaccgtt
     2941 caatatcaag taacgtccca aaccaaccaa gtgggcttcg tccatcccga tattgacctt
     3001 gcgagtttgt agtttagaca gagtggcaaa catgccatca atcaggtcat aaaccttttc
     3061 ttcgccgata aggaggatat cctccacatc acggagctcc tgaacttctt tgacactcca
     3121 tttaacaaag gccgacaagt gggccaaggt ctggatgcat ggtacaaagg tcatgtcaaa
     3181 ctgttgagca taggcttcga tttcctgcaa ctcctctgct gaataagctc cacggaaata
     3241 gccaaagtaa ggctgccctt caatctggta agtgtcttcc atgtaaagtt caaaggttga
     3301 gtagcccatg agagccaaga cctcaatcat ctgcttggca gaagccacat tcagcaccgc
     3361 atttcgagaa cagtcaacca tgtaagccaa atcttcgtaa gctgcttgtt cctcaatctc
     3421 tactttatca gcttctgcta gaactgttac caacaaggat aaggcacgat aaagttggtg
     3481 aggtttgcgg taggtcaatt gatagtgacc ttcctcaccc tcgatagaga tagaggcttg
     3541 gtcagactga gtgacagcca cttccacatc tggtagagaa atgtgacctt ttaaaacctc
     3601 aatagcttgc gtttgtttgg gactaagtcc tgtaaatctt accattgctt ttcctcctgt
     3661 agccagttga caagggcacc gtagagattg gcatctgcgt gataggtgca ggtctggata
     3721 actggtgcga ccgtgtattc ttcgtaggca tcgacaaagt cttcaacagc cttcttaaca
     3781 ccttggataa aatctggatt ttgactgata gagccaccca gactgatgac atctggatcg
     3841 atcagatact ggatattgag caagccttgc gccagattgc ggttcatgcg ctcaatggct
     3901 tcttgacaaa gagcattacc agttgcggcc tcttggtaaa tcttgcgacc gtcccaatca
     3961 gtatgaccag atttttcaat cacgtatcgt accatattcc cagttgatgc tagttgcgac
     4021 cagttattaa gtttttcagc aggggcaagg gttgtcatgt aaccaaattc tccacccaga
     4081 ccgtggcgac ctcgatgaag tctaccattg ataatcatgg ctccgccaat ccctgtccca
     4141 atcacgacac aggctgcatt ttcaagctct ggatgagcta gtagttcact gactccaacg
     4201 cagttggcat cattttctaa atggacaggt agctgataag agctaagcgc ctcataccaa
     4261 gaaaagccat ggatgtaggg caccgcactg aagccatcaa tcacacctgt ctcttgattg
     4321 accgcacctg gaacgctcat agcaatccca ctgtaatcct gttctgacaa gcgttgatct
     4381 agccacgcta gtaaatcctc caagttttca ggcgttgaaa tacttgtctt atccagtatt
     4441 ttcccatcag gagtcagact ggcaaactta atcccagtcc ctccgatatc aatcgttgca
     4501 atggtcattg tttttaccat aaaaaggggc gaatcagcag ttagttctta ctttttcgcc
     4561 cctcctttct tttgatgttt actttttgaa attaaatttc ttcttttttg atgaattctg
     4621 tacgaatctc ttgtggagcc aatagtcctg aatgaactgg atatggtcgc tcaagtaagt
     4681 caagaatggt ttgttgatgt tctgatatgc gcacattttc ttgactcata ttatagtaac
     4741 ggaggacata gccttcttca ttttccgcca ctttaaaggc tgttggacaa acttgcggta
     4801 tgctgagaac agaatggctc aagaggctac cagtcgcaac cacgcttcct tcctgtctag
     4861 caagctgaag gctggtaaac ggtgtctgca aggctttggc acgacgatag gctgagaagc
     4921 gttcttgggc ttggtggcat tcaagtgcaa actcgacttc aaactcccgc aagcattgtg
     4981 cttctggcgt tgggaagtag ccccagtcac ctagctcacc tgatgcacgc aaaatggtca
     5041 cggcaatggt gttatcccca aggatttcgt attcattcaa tcccttgttg gatacagtca
     5101 cacctttttc atcgtcatac agactaacaa aggcttgttg gtgttgagga ttttcaggat
     5161 tttcccatga agctgctggt ttgtttggtc gtgtcaccac ctcatagatg ctttcagaat
     5221 cattgcttgg acgcgtgtta tgagtcttga ccaagagacg gatacggtga tccttggcag
     5281 tgttggtaaa gcgagtcttg aagcggattt gtggattgtc aacgaagaca gtcaactcag
     5341 tttccagagg aatgtttgtc aattcttctg accgtccagc ctcacgcttc ataaactcga
     5401 tgataccttg ttgctcttct tctagctttt catccgcact gacaggcacg gtcaattcat
     5461 gtttgagcaa gattttagca tagcaagctg tgttttccaa gacctcgtgg cccttaagct
     5521 ctgcaaagat tggctctgtt ccttttggtt ggaaatagat atactcgttt ccgatgtcac
     5581 cccggtcttc gaagcggata aagtcttcat aggcttcgtg agttgtcttg tcatagactg
     5641 tgatgttgtc atccacactc accgttacga atggtgtatc aatcactccg ttttggtaaa
     5701 taccctcacg gtgttcttgt tttccttcca gcaattggaa ggttgtccaa gaaagcggcg
     5761 ctaggtgaac tggaatggtc acgcgcactt gacgagcaat acgagcttgg cggaacttgt
     5821 cttttggtaa atcatactca aaattagctc cgaggtcttc gattgtagcc tctacaggac
     5881 gaccatccaa gtcctccaca cggtaacttg gcaagataag agcagccatc tttttgtagc
     5941 cttctgttgg gtgcaattcc ttgaaatcac aagtcgccac atcaatcact gtgctgacag
     6001 tatcgacctt atcatgcaag cctgtgttaa tgacagtaaa gagatagtca ctttgagcct
     6061 tatccgtagc aattttaccc ttccactcgt tgagcaaatt acttttaaca aagtttccta
     6121 cttggttgac cttggcaaaa cgcgtttcca tctcgcggtg aacttcgtcc acgctacagc
     6181 cacagatact atcatgtggc gcattctgca aaagtgtttt ccaagcatag gtcaactggt
     6241 ccttgtggtt gtgtccacca gtgataatag tcaagggttc taccacttgc tctaggaggt
     6301 tgctattttc ttggaaggct tgttttaggt aaatgcggga tgaagaagtg ttggcaagtg
     6361 tgtaccagcc atctgtttcc tgactggtca actcacctgt aacagttgat aagtgttcag
     6421 gaagagcacc ttctacagct tgaacatatt catcaaaaga actatgaaca aagattacat
     6481 ccgggaagag ttcatttgcc acacgaatgg cttcgctcag atttttctgt acaggctggt
     6541 ggtcacagcc gttcatcatc aaccattggt tggtcgaagc gtaggcacgc acatctgaca
     6601 atttttgttt ccagaaggtc aaggcctcat ctttgtcaac tgggatttca ttcccattac
     6661 tgtaccagtt ggcaaagaga atacctaaaa cacgactacc atccacaccc tgccagtaca
     6721 tttctgaaaa ctgggatgta aactgctcat cttcaaggac ttggttgtca aatccaatcg
     6781 gcttcacacc acgaccaaag gccgccacgt gaatgcctga tttttgaaga atttgaggcg
     6841 cttgtcccat atttccaaag gtatctggaa agtagccaat ctgggttgat ttaccccatt
     6901 tggcagcttc ttgttgacca atcaaggtat tgcggacatt ggcttcactt gaaatcaaat
     6961 aatcatcctg caagatgtaa aagggaccaa ttttaagctt gccctcgtca atgtagcgtt
     7021 ggaccttgtc gcgattttca gggcgaattt gtaagtagtc gtcaaggaca atagtttgtc
     7081 catccaagtg gaaactcttg aactcagggt cattttcaaa gagatcaaag agattgtcaa
     7141 acaattccac caactgcata cgatgacttt caaaaggcaa gtaccactca cggtcccagt
     7201 gactatgtga gataatatgt acaacaacat tttccatgaa gtaaaacctc attctaaatt
     7261 taaattttaa cgttttaaat gtaaacttgt gattctgata agaatcggtt gagctaaagc
     7321 gagcccctta gcgaatatcc aagtaatcta agaccaactc acagaacatc atgttagccc
     7381 aggagaacca ttcacgagag tagagagttg ggtcatccac gtggaagctt tcatgcataa
     7441 gacctgtacc gccatcgcag gcaaccagct gatccagcaa gaagtttttc tctgccttat
     7501 ctcttgttgt caagccttgg atagaaaggg cgattggcca gatatagcga tagaaggtat
     7561 gagaacttcc gagaccgcta gcgtattctc cttggtagaa atatggattt tcagagctca
     7621 gaatggtacg gcgagtagct tgatacactt catcatcgac cgaacagtag cccagatagg
     7681 gcgcagctag tagacttggt acgtttggat catccataat gctggcattt cctaggccat
     7741 ctacttcaaa agcgtaaatc ttttcgccct tgctgttggt ggtgtaagcg tagttttcga
     7801 ttccttcttg gatttcatcc tgaagacgct tggcatcagc aataacactc tggctatcag
     7861 ctaggtttaa tgctgcgaag atttcttgca cataacccaa gactactaca gcaaacatat
     7921 ttgacggaat caagtaacta tactggcaac agtcatcact cggacgaaag gctgaccaag
     7981 tcattcctgt cactgcaaaa tcaggtccaa agccatcatt taccaaggtg tcttccttac
     8041 ggtccgtatc tcggacaaaa cgataaggag agttcttgtg gtcttgttca actgtccata
     8101 gatggagaat ttccttagtc gctacgacaa aaatctcatc aaactgacca gtctcgccag
     8161 tctctttcca gaggagataa gccaactgca aaggatagca aagtgaatcc acctcatact
     8221 tgcgctccca gatccagccg ttaaggtctg tgtggtcagt ctcgtggtgc cctttccagt
     8281 tctcctcaat gttgaaggag ttagcatagg gatccttgag taccaaggtc atctgacgtt
     8341 tgaccaaacc tgcaatggtc tgacgcagga gggcatctct tttagctaca tgaaggtagg
     8401 gtctgagttg ggctgtcgaa tctcgaagcc acatggcagg aatatcccca gtcaagacaa
     8461 aagttgaacc atcttctaag atttcaaccg tattgtccaa ggtatcggtg tagcaacgct
     8521 cgaaaacatc cacccactct ggatggtcct tagcccgctc tgctacttca tctagccatt
     8581 ctctaacaat ttctttcgaa taaatcatcg tgcaattccc tctttcaaac aagtttcatg
     8641 ttcagtatat agcttttgag acagaaaata catacacaaa tgatagtcat ttttctacaa
     8701 aatagttatc tttgaaactc cttttctaaa ggccctcttt ttctgatata atgtagaaaa
     8761 acagctaaag gaaagactat gaaaccacta cttgaaacca tcgatacccg ctttggtact
     8821 gctagcaagc atgccttctc tcggggaaat accctgccat acacaggcgt gccttttggg
     8881 atgaattact ttgtgcccca gaccagtgac caggatggat cttggttttt tgatccgcat
     8941 ctgcctatct ttcaggggat tcgattaact caccagccca gtccttggat tggggactac
     9001 tcttggctcc ttctgacacc tgtcaccagt caactgggtg gagacagcct ctttcaccgc
     9061 cagtcttctt atgatataga taaagcttgc ttccagcctc actatttgaa gctcttttct
     9121 ctgcgttatc agattgaaac ccagctcaca ccgacttgct atggtgcttc tattcgtttg
     9181 aatcaaaagc aaggcaaagc cctctccctc tatcttcacg cagcagatga actgaccgta
     9241 gagcaagtag ataagcggac tcttgccctg cgacaagaag gtaaaactga aactaacaaa
     9301 aattcgctaa cgatgttcac tgccctgcaa atgaacacgg atattcttgc tatcagccaa
     9361 gaagggggag actggcgaat tgacttagca agtagtcaaa ccgagatgca actagccact
     9421 tctttcatct ctccttctca agcactgctt aatctacctc aagaagattt tgatagctgt
     9481 aaagcaagtg cccaagcgga ttgggaaaat ctcctccatc gttttgacat tatagagaca
     9541 ggagaggctg accgaacctt ctttgaccac tgtctctaca gactcttcct cttcccacag
     9601 actttttatg aggttgatga gtcagggcaa gccatccaca tggatatggc tactggtact
     9661 gtcaagcctg gtgtcctctt tagcaacaat ggtttctggg ataccttccg caccaccttc
     9721 cccctctttg cccttatcat accggagcac tatcaacgct ttttagaagg tttcctcaat
     9781 agctaccgcg atactagttt ccttccaaaa tggctggctc cagatgaacg gggtatgatg
     9841 ccaggtacac ttttagacgg tattatcgca gatagcgcct gcaaggacat ggcccccgac
     9901 ctagaaggag aactcttcca agccatgctc gaaacagcca gcaaagccga ccctctcggc
     9961 atcaatggcc gccacggact agcccaatac caagaactag gttacctctc taccgaccac
    10021 cacgaaagtg ttagccatac tctagactat gcctatagtg acttttgtat cgccaactgt
    10081 gccaaaaaac taaaaaacat agaaatcgct gaaacctaca aggctgcttc acaaaattac
    10141 cgccagctat ttgacgctga gacaggttac atgcgagcac gagacaatca aggaaacttt
    10201 caccctgact tctctcctta tagttggggg cgagactacg ctgaatgctc tgccattcaa
    10261 gctactttag gcgttctcca cgacatccct ggcttaatcc aactgatggg tggaaaagaa
    10321 acctttagca actatctttt gaaagcctgt caagatgctc ccctctttga aacaacaggc
    10381 tatggttacg aaattcacga aatgagcgag atggctactg ctccttttgg acaaatagcc
    10441 atttccaacc agcctagttt ccacattcct tatctcttcc gctacagcga ttaccctgac
    10501 tacactgccc ttcttatcaa gacgctccgt cagaaagctt ttcacccaag ttgggaagcc
    10561 tatcctggag atgaagacaa tggcagtctc tctgcttggt acatctggtc agctctcgga
    10621 ttttatccaa cctgtccagg aaaaccaagc tatgacctcg gaatccctct ctttgaccat
    10681 ctacgagtct acctcgctaa agaagataaa tggctggata tccatactaa acaaaaccac
    10741 aaccatttta actttgtcaa agaatgccga ctggacaaaa tactcgtatc aactattcaa
    10801 caccaagacc tcttaaaagc tgaacaacta actttcaccc tcagctggct accaaatcac
    10861 taataaccaa aagaaccttt gcatcgcgca aaggttcttc ttttatgaaa cttggacttg
    10921 aagttgatcc acaagctgac catccaccgt taggttcaga tagaaatcca aggttttatc
    10981 tcctgcgaaa tacaaggttg gtagcgtcag acttttttca gtctcaccag cttgaaacgc
    11041 aaggacttga atctcatcct gataggcgac accatggaca cctgtccccg gttgaatcga
    11101 aatcttagct tctaaagagg agctactttc actacgttca atcctaaaat gaagagtctc
    11161 tccctttgcc acagctaggc ttttttctgc aaatgctagt tcctgaacaa cttctttttt
    11221 tgataatcca ggagttttat aaagggaaat cttggtcaat acagggatag attgtgcctc
    11281 ggtaatcatc acgcgcacct tttgtgcctc tactagcgga cctcgcaaga ggcgcttatg
    11341 accaactgtg aagcccctgc caaactcctg ccagacacca tccacctcta cttgcacatg
    11401 aaaagcagcg attcgttgcc ctagcttcaa atcttctctt aactcaatta catcaaaagt
    11461 tttaggagaa cctaaatcga gttctaactg gatgggcaag tctgcatcgt ttgcccaaga
    11521 gctggtctca aggccgtctg tcaaatggcg acaagcaaag tctgcggaaa gagctggacc
    11581 agatacctca gctcccagag ccaaatcttc tttatagagc tcattgcgat aggtcgcaaa
    11641 ttcataaagt cgttcaatat cctttgcatc aaagagccca gcttgattcg gcggaatatt
    11701 aagcaagagt ggagttcctc gccctactga gtgaaagtag atttcgacca actcctcgag
    11761 agacttagga tcctgatcct catggtagaa ccagcctgga cggatggaaa catctgcctc
    11821 tccgattgaa aaaatcgtgc ccgagggatc cccgtgctga agatagttca gctctgcttc
    11881 tgttcctagt ttatcaggat tcaccttttg ccacagtgga tcacctgcat accctcgttc
    11941 attgccaatc cagcggatac tggtgccttc tgttgaaaaa atcaagcaag cgccctgcag
    12001 gtcacgaatg gtttcaaacc atttttcaaa ttcataatta accttttgcg cgccctctcc
    12061 tctggcacca tccatccaaa cctcagcgaa cttaccagca ttcccatagt taggatttga
    12121 taagatttcc ttcaactgag ccagataata ggcattgtag tccgcttctc ggtccacatg
    12181 atagagggga ctatgggcat cccacggtga caggtagacc cccatatcca tatcaaactc
    12241 tgtggcagct tgggatactt caaggagcaa gtcgcccttt cctctcctcc aaggactgac
    12301 cttaaccgaa taatctgtgt gagctgtcgg ataaaggaca aagccatcgt ggtgcttgac
    12361 caccaaaatc aactttttga agcccgtttc cttgagcaca cgaacccact cacgcgcatc
    12421 caactgactc gggttaaagc gctcaggatc ctcctgtcca gtcccccatt cttggtcata
    12481 aaaggtatta ggaccgaagt ggataaaagc tgctagttca tctcccagat aagctagctg
    12541 agtctgactt ggtaacggtc catgcggttt gattttcttc atcgttatat atctccttct
    12601 ttagcaactt ctcttgttca caaggttatc cacaggttgt ggataataaa aaatgatcta
    12661 accgccagct ctttccaact ccaactggta ggctcaggat ttttctcctc aatattccct
    12721 cttcaaacag ctctgtcata tggttctgct aacacctctc agaatgctac tctccataat
    12781 tattctcagg cttctttcag gtcattgtgt gaagaccatt cactctccag acttttccac
    12841 agattgtgaa taagtgatta ttctaaacta tcacctcgtc gactataaca aaaaataaaa
    12901 agagggtcaa taagataatt atctcttcct aaatactcag aaaagattct ataatttgta
    12961 caattgagca agaaatgctt ctccaaaatc tatcttcaaa agaatgttgc atctttttat
    13021 cctttcacac aaacatttct ctttatacag atccaagtga taaccagatg ataattggac
    13081 actttttggt catttgtata ttttttgaaa aattttttaa aaaaatttta tatccttttt
    13141 atagaagttt atgcatttgt atctatgtat tctatcctat gttatgtata ataacataaa
    13201 attgtgctaa acatcataat ttatattatt ttccgagaaa ttatagccat tgacattacc
    13261 taggaattgc gttataacat acatgtaagc ggtacccaaa atggtgccaa gtcaaaattt
    13321 ttaaggagga aaatacatgt cttctcatcc aattcaggtc ttctcagaaa ttgggaaact
    13381 gaaaaaagtt atgttgcacc gtccaggcaa ggagttagaa aacttgttgc cggactatct
    13441 tgaaaggctt ctttttgatg atattccttt cttggaagat gctcaaaaag aacatgatgc
    13501 atttgcccaa gctcttcgcg atgaaggaat tgaggttctc tacctagaac aactcgctgc
    13561 tgaatcattg acctctccag aaatccgcga tcaatttatc gaggaatact tagacgaagc
    13621 caacatccgt gatcgtcaaa ccaaggttgc tattcgtgaa ttgcttcacg gcatcaagga
    13681 caaccaagaa ttggttgaaa aaacaatggc tgggattcaa aaagttgaat tgccagaaat
    13741 tcctgacgaa gctaaagatc taactgactt agttgaatca gattatccat ttgcaattga
    13801 cccgatgcca aacctctatt tcactcgcga cccatttgca acaattggaa acgccgtatc
    13861 gcttaaccac atgtttgcag acactcgtaa ccgtgaaaca ctctacggta agtatatctt
    13921 caaataccac ccagtctatg gcggaaaagt ggatttggtc tacaaccgtg aagaagatac
    13981 gcgtatcgaa ggtggagacg agctagttct ttctaaagac gtccttgcag taggtatctc
    14041 tcaacgtaca gacgcagctt ctatcgaaaa acttttggtc aacatcttca agaaaaatgt
    14101 tggcttcaag aaagttttgg cctttgaatt tgctaacaac cgtaaattca tgcacttgga
    14161 tactgtcttc actatggtag actatgacaa gttcactatt cacccagaaa tcgaaggcga
    14221 ccttcacgtt tactcagtta cttacgaaaa cgaaaaactt aaaatcgttg aagagaaagg
    14281 tgacttagct gaacttcttg ctcaaaacct tggtgtagaa aaagttcatt tgattcgttg
    14341 cggtggtggc aatatcgtag cagctgcgcg tgaacaatgg aacgacggtt ctaacacttt
    14401 gaccatcgca cctggtgtgg tagttgttta tgaccgcaat accgtgacca ataagatttt
    14461 ggaagaatac gggcttcgct tgattaagat tcgcggaagt gaattggttc ggggccgtgg
    14521 tggacctcgt tgtatgtcta tgccatttga acgtgaagaa gtgtaatcgc tgttcggtat
    14581 tcgtcaatag aaaatgtaaa aaatagaaag aggaaataat aaaatgacaa attcagtatt
    14641 ccaaggacgc agcttcttag cagaaaaaga ctttacccgt gcagagttag aataccttat
    14701 tggtctttca gctcacttga aagatttgaa aaaacgcaat attcaacacc actaccttgc
    14761 tggcaagaat atcgctctcc tatttgaaaa aacatctact cgtactcgtg cagcctttac
    14821 aactgcggct atcgaccttg gtgctcaccc agaatacctc ggagcaaatg atattcagtt
    14881 gggtaaaaaa gaatctactg aagatactgc taaagtattg ggacgtatgt ttgacgggat
    14941 tgaattccgc ggattcagcc aacgtatggt tgaagaattg gcagaattct caggcgttcc
    15001 agtatggaac ggtctaactg acgaatggca cccaactcaa atgctcgctg actacttgac
    15061 tgttcaagaa aacttcggtc gcttggaagg cttgacattg gtatactgtg gtgatggacg
    15121 taacaacgtt gccaacagct tgctcgtaac aggtgctatc cttggtgtca atgttcacat
    15181 cttctcacca aaagaactct tcccagaaaa agaaatcgtt gaattggcag aaggatttgc
    15241 taaagaaagt ggcgcacatg ttctcatcac tgaagatgct gatgaagcag ttaaagatgc
    15301 agacgttctt tacacagacg tttgggtatc aatgggtgaa gaagacaaat tcgcagaacg
    15361 tgtagctctt cttaaacctt accaagtcaa tatggactta gttaaaaaag caggcaatga
    15421 aaacttgatc ttcctacact gcttgccagc attccacgat actcacactg tttatggtaa
    15481 agacgttgct gaaaaatttg gtgtagaaga aatggaagta acagacgaag tcttccgcag
    15541 caagtacgct cgccacttcg atcaagcaga aaaccgtatg cacactatca aagctgttat
    15601 ggctgctaca cttggtaacc tttatattcc taaagtataa ttttagataa taaaccgtct
    15661 accaacagct atgagggctg cgactaatag ctttagtccg gtcctctttt atgtaatggt
    15721 aatctattat ttcttataaa atatgtgaaa aatcattaaa ttgaaatcta aacgcattct
    15781 attgagtgtg ataaaggaga atttatggca aatcgtaaaa ttgtagtagc tttgggagga
    15841 aatgcgattc tttcttctga cccatcagca aaggctcaac aagaagcttt agttgaaaca
    15901 gctaagcatc ttgtaaaatt gattaaaaat ggagatgatt tgattatcac tcacggtaat
    15961 ggacctcaag ttgggaatct cttgctccaa catttggcat cagactctga aaagaaccct
    16021 gccttcccac tcgactcact tgtcgctatg acagaaggta gcatcggttt ctggttgaaa
    16081 aatgctttgc aaaatgctct cttggatgaa ggcatcgaaa aaaatgttgc ctctgttgta
    16141 acgcaagtta tcgtagataa aaatgatcca gcttttgtta acttgagtaa accaatcggt
    16201 cctttctatt cagaagaaga agcaaaagca gaagccgaaa agagcggagc gactttcaag
    16261 gaagatgctg gccgtggctg gcgtaaggtc gttgcctcac caaaacctgt tgacatcaaa
    16321 gaaattgaaa ccatccgtac tcttttaaat aatggtcaag tcgtcgtagc tgcaggtggt
    16381 ggcggtattc ccgtcgtcaa agaaaacaat ggacatttga ctggtgtcga agcggttatt
    16441 gataaagact tcgcttccca acgtttggca gaattggttg atgcagacct cttcatcgtt
    16501 ttgacaggtg tagattatgt atttgttaac tacaacaagc caaaccagga aaaattggaa
    16561 catgtgaatg ttgcccagct ggaagaatat atcaaacaag atcagtttgc accaggtagc
    16621 atgcttccaa aagtagaagc agctatcgct tttgtcaatg gtcgtccaga aggaaaagca
    16681 gttattactt cccttgaaaa tctaggcgcc ttgattgaat ctgaaagcgg aacaattatt
    16741 gaaaaaggat aagttgtttt actaataaga tgtattctat ttctagtatc tttatatcaa
    16801 attagaaatt attcttgaaa acatgtacaa tatttcaaaa gatactagtt ttagacttta
    16861 atatggtaaa acaaatataa atagaaagcg ttttcttgaa tgtttattta agaaagtagt
    16921 tggtttttta cactttgtta gacatcagga ggaaaaacaa atgagtgaaa aagctaaaaa
    16981 agggtttaag atgccttcat cttacaccgt attattgata atcattgcta ttatggccgt
    17041 gctaacttgg tttatccctg cgggggcctt tatagaaggt atttacgagg ctcagcctca
    17101 aaatccacaa gggatttggg atgtcctcat ggcaccgatt cgggctatgc taggtactca
    17161 tccagaggaa ggttcgctca ttaaagaaac gagcgcagcg attgatgtag ccttcttcat
    17221 ccttatggtt ggtggtttcc ttggcattgt caacaaaact ggtgctcttg acgtagggat
    17281 tgcctctatc gtgaagaagt ataagggccg cgaaaaaatg ttaattttgg tattgatgcc
    17341 tttgtttgcc ctcggtggta caacttatgg tatgggtgaa gaaacaatgg ccttctatcc
    17401 actccttgtg ccagttatga tggccgttgg ttttgatagc ctgactggtg ttgcaattat
    17461 tttgctcggt tctcaaatcg gctgtttggc atctactctg aatccatttg cgacaggtat
    17521 tgcttcagcg actgcgggag ttggtacagg ggacggtatc gtacttcgtc tgatcttctg
    17581 ggttaccttg actgctctta gtacttggtt tgtttaccgt tatgcggata agattcaaaa
    17641 agatccgact aagtcactgg tttatagtac tcgcaaagaa gatttgaaac actttaacgt
    17701 agaagaatct tcatctgtag aatctacact tagcagcaaa caaaaatcag ttctcttctt
    17761 atttgtgttg acattcatct tgatggtatt gagcttcatt ccatggacag accttggcgt
    17821 taccattttt gatgacttta atacttggtt gactggtctt ccagttattg gtaatattgt
    17881 cggttcatct acttctgcac taggtacttg gtacttccca gaaggcgcaa tgctctttgc
    17941 ctttatgggt atcctgattg gtgttattta tggtcttaaa gaagataaga ttatctcttc
    18001 cttcatgaat ggtgctgctg acttgctcag tgttgccttg atcgtagcga ttgctcgtgg
    18061 tattcaagtt atcatgaacg acggtatgat taccgataca atcctcaact ggggtaaaga
    18121 aggcttgagc ggtctatctt cacaagtctt tatcgttgta acttatatct tctatctacc
    18181 tatgtcattc ttgatcccat cttcatctgg tcttgccagc gcaactatgg gtatcatggc
    18241 tccacttgga gaatttgtaa atgtccgtcc tagcttgatt atcactgctt accaatctgc
    18301 ttcaggtgtc ttgaacttga ttgcaccaac atctggtatt gtgatgggag ctcttgcact
    18361 tggacgtatc aacattggta cttggtggaa attcatgggc aaactcgtag tcgctattat
    18421 tgtagtgacc atcgcccttc ttctccttgg aaccttcctt ccattcctat aaaatagtga
    18481 gtgaggtgat tccatgaaaa tagatataac aaatcaagtt aaagatgaat ttcttatatc
    18541 attaaaaacc ttgatttcct atccttcagt actcaatgaa ggagaaaatg gaacaccttt
    18601 tggacaagca atccaagatg tcctagaaaa aactttagag atttgtcgag acataggttt
    18661 cactacctat cttgacccta aaggttatta cggatatgca gaaatcggtc agggagcaga
    18721 gcttctggcc attctctgtc atttggatgt tgttccatca ggtgatgaag cagattggca
    18781 gacaccgcca tttgaagcaa ctatcaaaga cggctgggta ttcggacgtg gtgtccaaga
    18841 tgataaaggc ccttcgctcg cagctctcta tgcagtaaaa agcttgctgg accaaggtat
    18901 tcagttcaaa aagcgcgtac gctttatctt tggtaccgat gaggaaaccc tctggcgctg
    18961 catggcacgc tacaatacca tcgaagaaca ggccagtatg ggctttgcac ctgactcatc
    19021 ttttcctctg acctatgctg aaaaagggct tctacaggtc aaacttcatg gccctggatc
    19081 ggatcaacta gagcttgaag taggaggcgc ctttaacgtt gtaccagaca aggccaacta
    19141 ccaaggtccc ctctatgaac aggtttgtaa cggtctcaaa gaagctggtt atgattacca
    19201 atccactgaa caaaccgtaa cggttctcgg agtgccaaag catgctaagg atgctagtca
    19261 aggtatcaat gctgtcatcc gactagctac cattcttgct cctctccaag aacaccctgc
    19321 tctcagtttt cttgcaacac aagcaggtca agacggcaca ggaagacaaa tctttggtga
    19381 tatagcagat gaaccttctg gtcacctatc ctttaatgtc gcaggtctca tgatcaatca
    19441 tgaacgttct gaaatccgta ttgacattcg gactcctgtc ttagctgaca aggaagaact
    19501 agtagagttg cttacaagat gtgcacaaaa ctaccaactc cgctacgaag agtttgacta
    19561 tctagcgcct ctatacgtcg cagaagacag taaactcgtt agcacactga tgcaaatcta
    19621 ccaagaaaag actggcgata acagtcctgc tatttcatcc ggtggtgcca cttttgctcg
    19681 caccatgcca aattgtgtag ccttcggcgc cttattccca ggagcgaagc agacagaaca
    19741 tcaggcaaat gaatgtgccg ttctagaaga tttgtaccgt gctatggata tttatgccga
    19801 agccgtctat cgacttgcaa cttaatcagg caactgtttc taccaaaaaa aatcgaccga
    19861 ttaatgaact gcaccccaaa agttagacag aataaatcta acttttgggg tgttttatta
    19921 tgaaattgag ttatgaagat aaagttcaga tctatgaact aagaaagcaa ggacaaagct
    19981 tcaaacagct ttcaaaaaga tttggtgtgg atgtttctgg tctaaagtca tctgaatctt
    20041 tgagatgagc tttataaatc gcttttttca gtttttgcac tggtgtttcg ataaactcaa
    20101 actttttaac cgtggtattg cctgatttta tagtatattg aaactagaat agtacacctc
    20161 tacttctaaa acatttttag aaatcgattt gactgtcctg atcgatttgt cctgttctta
    20221 tttcatttta ctatatttga gccacttcgt ctttaacggc tttattcata agctcttgta
    20281 at
//
LOCUS       DAVMAF010000031        18680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMAF010000031 DAVMAF010000000
VERSION     DAVMAF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.031"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(233..1039)
                     /gene="yidA"
                     /locus_tag="VYO97_001158"
     CDS             complement(233..1039)
                     /gene="yidA"
                     /locus_tag="VYO97_001158"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1753961.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1052..2356)
                     /locus_tag="VYO97_001159"
     CDS             complement(1052..2356)
                     /locus_tag="VYO97_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1753962.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2428..2805
                     /locus_tag="VYO97_001160"
     CDS             2428..2805
                     /locus_tag="VYO97_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1753963.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKRDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYILTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2896..3228
                     /locus_tag="VYO97_001161"
     CDS             2896..3228
                     /locus_tag="VYO97_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1753964.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3240..4811
                     /gene="ffh"
                     /locus_tag="VYO97_001162"
     CDS             3240..4811
                     /gene="ffh"
                     /locus_tag="VYO97_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1753965.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5006..6289)
                     /locus_tag="VYO97_001163"
     CDS             complement(5006..6289)
                     /locus_tag="VYO97_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1753966.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSIALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6592..7305)
                     /gene="rsmG"
                     /locus_tag="VYO97_001164"
     CDS             complement(6592..7305)
                     /gene="rsmG"
                     /locus_tag="VYO97_001164"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1753967.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7399..7959
                     /locus_tag="VYO97_001165"
     CDS             7399..7959
                     /locus_tag="VYO97_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1753968.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7961..8860
                     /gene="htpX"
                     /locus_tag="VYO97_001166"
     CDS             7961..8860
                     /gene="htpX"
                     /locus_tag="VYO97_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1753969.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRIFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8904..10463)
                     /locus_tag="VYO97_001167"
     CDS             complement(8904..10463)
                     /locus_tag="VYO97_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1753970.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRLLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10702..11019)
                     /locus_tag="VYO97_001168"
     CDS             complement(10702..11019)
                     /locus_tag="VYO97_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1753971.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            11189..11731
                     /locus_tag="VYO97_001169"
     CDS             11189..11731
                     /locus_tag="VYO97_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1753972.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11731..12360
                     /gene="nth"
                     /locus_tag="VYO97_001170"
     CDS             11731..12360
                     /gene="nth"
                     /locus_tag="VYO97_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1753973.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12571..13092
                     /gene="pyrR"
                     /locus_tag="VYO97_001171"
     CDS             12571..13092
                     /gene="pyrR"
                     /locus_tag="VYO97_001171"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1753974.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13111..14034
                     /locus_tag="VYO97_001172"
     CDS             13111..14034
                     /locus_tag="VYO97_001172"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1753975.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14084..15163
                     /gene="carA"
                     /locus_tag="VYO97_001173"
     CDS             14084..15163
                     /gene="carA"
                     /locus_tag="VYO97_001173"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1753976.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15476..18652
                     /gene="carB"
                     /locus_tag="VYO97_001174"
     CDS             15476..18652
                     /gene="carB"
                     /locus_tag="VYO97_001174"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1753977.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5432 a         3605 c         4196 g         5447 t
ORIGIN      
        1 gaagagagta tttaaaatat cttgggaaat tataatttct ccctgttttt tgagtccaat
       61 ctatgagtat gagttgccca ggagtaaggc cacattctaa ttcgtttagg taaaaattgt
      121 tacaatcaga atttgtgtct ctattatgat tattttgttt taaattaaat agtttgctaa
      181 aaaaattcat tggattttat tgctaatcaa ttcttattga aaaatgatac ttttacagta
      241 cccatgttcg gatggcgtgg gcaacgccgg attcgtcatt tgttttcgtg atgtatttgg
      301 cgattttttt gatttctgga tttccgtttt ccatgactac agggttgcca acgacttcca
      361 gcatggcacg gtcattttct tcgtctccga tagccatggt ttcatctttg gttaatccta
      421 gtttttcagc caagtgagta atagctgaac ccttgtctac attcttttta aggagttcga
      481 ggtagaaagg agcagatttg ttgatggagt agcgttcgta aaattctgct ggtatctttt
      541 caatcgcagc atcgagaatc tctggttcat caataaacat acatttgacg atttccttgc
      601 cagccatttc ttcaggagtg cggtagaaga taggcatgct gacgagggtt gattcgtgta
      661 cagtgtatgt tccgatattg cgatttgcag tatagatacc gtccttagta atagcatgca
      721 tgtgaacacc aagcttgcga ctgagaaatt ccatatccag ataatcctca taggtcaagg
      781 attcgctgat aatctcatgg ccagtagcag tttcttggac aagggcaccg ttgaaggtca
      841 ccacatagtc accttggtct ctcaactgca agtcgtccag aagtttggca acacctgcga
      901 tggggcggcc agttgcaatc acgactttga caccagcttc tttggcatct tggatggcag
      961 aaaagacttc aggagtgatt tccttttggc tgttgactag ggttccgtca atatcgacgg
     1021 cgattagttt aatactcata aatttcctct tttagttttt atttggggta aaatgatcgt
     1081 tctcaatcaa gtgtaaaaat tgctgggtga tgcttgcgaa gatgctgttt tggtctaaca
     1141 tttctttcgg gaaataaaag cgattatctc cgtggcgact tccagcaagg gattggacga
     1201 taggggacag gctagagagt tctgccagtt ctccattttt ttgtaaaatc tcaatctgtg
     1261 tccttggatt ttcagattcg ggacggtaaa tatcataagg gaggtcaaag ttcttatgaa
     1321 tggcagtata gtagtcggga tcaaagccga tgtcctcaac caattttctc atgctagcga
     1381 gttggtcttg gtcttcttgt gaaaaggtaa tggatttaaa gaccttgcgg ttgacaaagc
     1441 gttgtgacaa gtctgcgaga atcttgtcag gactggtcat ccagagttga aagtaggtat
     1501 tcatcacacc atcatctaga gccagatagt cagtcaaggt cacatttttt tcaaagaaag
     1561 gcaggaggtg tggagaagtt cgtgcaaaga agtccttgtc ttcaggatag agttccttgg
     1621 cgcgtttgag gagattctgc aggagaactt ccatggcgcg tgttgcgggg tggaaataaa
     1681 cctgcatgta catctggtag cgactgagga cgtagtcttc gatggcgtgc atgccattgc
     1741 gctgaaaggc gataccattt tcgataggac gaatgactcg gaggatgcga gtcaggtcaa
     1801 attccccata ggatgctcct gtaaaatagg agtcgcgcaa gagatagtcc atgcgatctg
     1861 cgtcaatctg actagaaatg agctgcacga cctgcttatt aggataggta tggtcaatga
     1921 cactggccac cttttctggg aaatcgggcg ctacttgtag caagacttgg tgaatctctg
     1981 tctcaggatt ttgaataatc tcctgagtaa tggcttcatg gtctgtatca aagagatgtt
     2041 caaaagtatg ggagtaggcg ccatgcccaa ggtcatgtag gagagcagcg gtcatggtca
     2101 agagagactc ggcaggattc cattcctcag gatatttttc ttcaaaaatt tctgtgatac
     2161 gtcgtgcaat ctcatagact cccagacagt gagagaagcg actatgctct ccaccgtgga
     2221 aggtataact ggaagtccct agttgcttga tccggcgcaa acgttgaaat tcttttgtat
     2281 taatcaagtc atagatgatt tgattattga catggatgta gttgtgaact gggtcacgga
     2341 atactttttc gttcatatga ccattatagc aaaatcctgc tctttttggt aaaatagtag
     2401 gacaagagac aagaaagagg acctgatgtg aagattcgga tgcgaaatac aattcagttt
     2461 gatgagcagt tggaagtgat tgaccagctt tatgatgtgg aagtgcatga aaaaagagat
     2521 tatagctacc tgcttttcta taatgaggaa aaggaaaaag tagtgattaa atttcatggt
     2581 caagaacttg tgatgagccg attttctaat cccaagacca ttatgcgctt tctaaaggat
     2641 agcgatagtt tagcctatat tcttacacct atgggcatgc aggagtttac catccaaacg
     2701 agccattatc aagttgatag gcaaaagatt gaactagatt atcaactaca aaatcaagag
     2761 ggacatccct ttgccagcta tcaattggaa attacttggg gctaggctca tgtctttttc
     2821 aaatttagct atctatcttc ttggatcggc ttcctagtgt ggtagattag gctaattttt
     2881 ggtataataa aagttatgga aatcgaaaaa accaatcgta tgaatgcgct ctttgaattt
     2941 tatgcggcgc ttttgacaga taagcaaatg aattatatcg agctctacta cgctgatgat
     3001 tacagccttg ctgaaattgc cgaggagttc ggtgtcagtc gtcaggctgt ctatgacaat
     3061 atcaagcgaa cagaaaagat tctggaagat tatgagatga aattgcatat gtactcggac
     3121 tatattgtcc gcagtcagat ttttgatcag attttggagc gctatcccaa ggataacttt
     3181 ctgcaggagc agatagaaat tttaacaagc attgataata gagaataaga ggaagaaaaa
     3241 tggcatttga aagtttaaca gaacgtttgc agaacgtctt taaaaatcta cgtaaaaaag
     3301 gaaaaatctc tgaatctgat gtccaagagg caaccaaaga aattcgcttg gccttgctcg
     3361 aggccgacgt tgccttgcct gttgtaaagg actttatcaa gaaagttcgt gagcgtgcag
     3421 tcgggcatga ggtcattgat acacttaatc ctgcgcaaca gattattaaa atcgttgatg
     3481 aggaactgac agccgtttta ggttctgata cggcagaaat tatcaagtca cctaagattc
     3541 caaccatcat catgatggtt ggtttacaag gggctggtaa aacaaccttt gctggtaaat
     3601 tggccaacaa actcaagaaa gaagaaaatg ctcgtccttt gatgattgcg gcggatattt
     3661 atcgtcctgc tgccattgat cagctgaaga cactgggaca acagattgat gtgccggttt
     3721 tcgctcttgg gacagaggtt ccagctgttg agattgtacg tcaaggtttg gagcaagctc
     3781 aaactaatca taacgactat gtcttgattg atactgcggg tcgtttgcag attgatgagc
     3841 tcctcatgaa tgagcttcgt gatgtgaaag cattggctca accaaatgaa atcttgcttg
     3901 tcgttgatgc tatgattggt caggaagcag ccaatgttgc gcgtgagttt aatgctcagt
     3961 tggaagtgac tggggtcatc cttaccaaga ttgatggcga tactcgtggt ggtgctgctc
     4021 tgtctgttcg tcacattact ggaaaaccaa tcaagttcac tggtacaggt gaaaagatta
     4081 cggacattga aaccttccac ccagaccgca tgtctagccg tatccttggc atgggggata
     4141 tgctcacttt gattgagaaa gcttctcagg aatacgatga acaaaaagcc cttgaaatgg
     4201 ctgagaagat gcgcgaaaac acctttgatt ttaatgattt catcgatcaa ttagatcagg
     4261 tgcaaaatat ggggccgatg gaagacttgc tcaagatgat tccaggtatg gccaacaatc
     4321 cagcccttca aaacatgaag gtggatgaac gccagattgc tcgtaaacgt gccattgtgt
     4381 cttcgatgac acctgaagag cgtgaaaacc cagatttgtt aaatccaagc cgtcgccgtc
     4441 gtattgctgc tggttctgga aatacattcg tcgaagtcaa taaattcatc aaggacttta
     4501 accaggctaa acagctcatg cagggtgtta tgtctgggga tatgaataaa atgatgaagc
     4561 aaatggggat taatccaaat aaccttccta aaaatatgcc aaatatggga ggaatggata
     4621 tgtctgccct tgaaggaatg atgggacaag gcggtatgcc tgacttatca gctctcggag
     4681 gagcaggaat gccagatatg agccagatgt ttggtggcgg tttgaaaggt aaaattggtg
     4741 aatttgccat gaaacagtcc atgaaacgta tggctaacaa aatgaagaaa gcgaagaaga
     4801 aacgcaagta agacaagaga ttgatgcaaa tctcatttga tgagaaaata aagaaaaaat
     4861 ttctatgata aaacgtacag tatcaaggtt ttagaatatc tgatattata cgttttttga
     4921 ttttaataat tttgttaaga cacttttatt atggtatgcg aaaaatccac tcagctgtct
     4981 gagtggatta ggtgtattta gactcttagt ctttattttc gtatggcaag atcaagttca
     5041 agatgattcc tgtcatggct gaaagggcag tacctgaaag tgtaactgga ccaagtttaa
     5101 ggatagctcc tccaagtcca agaaccaaca tagcacttgc gatgatgagg tttcgcattt
     5161 gagcgaaatc aacacgttct ttaatcaaga ctttcaaacc attgctggcg ataaccccat
     5221 agagaaggat tgacatacca ccaagtacag cgtttggaat agttgaaatc aaggcagtga
     5281 atttaccaag gaagctgagg gcaatcgcga tgaaggcagc gttacggata actgagacag
     5341 aagcgatacg agtcataccg ataacccctg tattttctcc gtaagttgta ttggctggtc
     5401 caccaaggaa ggcagaaaca gaagttgcga taccgtcacc aagaagagta cggtgaagac
     5461 ctggttcttt taagaattga cgaccacaga tttgacccaa aacagtatgg tctccgatat
     5521 gttcagaaat tgttacgata gcgattggca agatagcgat ggcttctgga ccaaagtaaa
     5581 gattgtactc tttaaaggca ccacctgtgc taaatggcaa gtagaaacca ggaatttcga
     5641 accagttggc tttaagaact ggtgtaaagt caaccaagcc aagagttagt gcgaaaaggt
     5701 aaccaccgat aatggcaaag aggaatggaa tgattcgtag gaagcctttt ccttttgtat
     5761 tgataaaggc agcaattagg aaagtaacaa cggctaccag agcatttttc caatttccgt
     5821 ctgctacaag acctgcattg gtaacagctg aacctgcaag tccaagaccg ataacgatga
     5881 tcataggacc gataatgatt ggtggcaaga gtttatcaat ccattttgtt cctacaaatc
     5941 ggatgctggt agcaacaagg acatagacca aaccagtcaa gataacccct gtttgggcag
     6001 cagatacatc cccccccatt tctttcatag ccagtgacat agctgtgata aaggcaaatg
     6061 aagaacctag ataaactgga actttaaaac cagtagcaat catgtagatg agtgttccaa
     6121 cacctgaagc aaaaagggca atagatacag gcattcccaa aatcaatggt accaagatgg
     6181 tcgcaccaaa catggcgaaa acgtgttgga aactaaggag aattcctttt ccatccgaag
     6241 gacgttggtc aacgtctagt aacaaatcaa cagttgattc ttgtttcata taaacctcac
     6301 ttttgggcat cagaaaagag cccattattt tacagcaata ggccctcaga gtaagacttc
     6361 tctaaaatct agactttaaa gagtttcaaa tatttctgcg tcttcgtcac ctcacgggat
     6421 gacattaaaa acctttgctt aggatagtat agcagaaaaa aatgattttg taaatcattt
     6481 tttcccgagc ctagaaataa aagagcgagg ctgattttgt aaatcatttt ttcccgagcc
     6541 taaaaataag agagcgaggt tgattttgta aatatttttt tacttaaaaa tttaaagcgg
     6601 gcgtttattt ggcataccag ccttacgtgg atatttattt ggtgtttctt ttttcttttc
     6661 taccactgtg atgtaacgcg gatctctatt tggtagggcg tagctgagat tgtcttcgac
     6721 tttactaaaa aggagattga gggcattctt agcttctaac aactcctcag gcgcattgct
     6781 ggccttgagt gccaatagtt ttccaccaac cttaaggtag ggaatagtca attcagatag
     6841 gacctgcata cgggcaaccg cacgagctgt tacaaaatca tattgagcac ggaagttctt
     6901 gtcttgggca aaatcttcgg cacgtccgtg gtagaaatga actccgttca aatccagttc
     6961 ttgagccaag agttgtagga agttgatgcg cttattgagt gaatcaataa tggtcacatc
     7021 taactccgga tagagaattt tcattggtag actaggaaat cctgccccag ccccgatatc
     7081 aagaagtttg atagtttcat tgggaatcaa accttgaaga atgggtgcaa tcgaatcgta
     7141 aaaatgtttg agataaactt cttccttgtc cgtaatcgcc gtcaaattaa tcttctcatt
     7201 ccactcgacc aagagctcaa aataacgttc aaattgttct ttttgctggt ccgaaagtgg
     7261 aagattctgc tcggcaagca agttgtaaaa tgtttttggt ttcatagtta tttccttctt
     7321 tagtatcatc ttattttacc acaatcatag aaaatttgat atactggtac taatctaaaa
     7381 gcagaaagga cttatattat gacttggatt attcttggag ttttagctct tgttgttatt
     7441 tttgtgattg ttagctataa cggtttggtt aaaaatcgta tgcaaaccaa ggaggcttgg
     7501 agtcagattg atgttcagtt gaaacgtcgc aatgatctct tgccaaactt gattgagact
     7561 gtaaaaggtt atgccaaata tgaaggttct acccttgaaa aggtggcaga actacgtaac
     7621 caagtggcgg cagcgacttc accagcagaa gctatgaaag ccagtgatgc cctcactcgt
     7681 caggtttcag gtatttttgc agttgcagaa agctatccag atttgaaagc tagtgctaac
     7741 tttgttaaat tgcaagagga gttgacaaac acagaaaata aaatttctta ctctcgtcaa
     7801 ctctataaca gtgttgtcag caactacaat gtaaaattag aaactttccc gagcaatatt
     7861 atcgctggaa tgtttggatt taaagcggca gatttccttc aaacacctga agaggaaaag
     7921 tcggttccta aagttgattt tagcggttta ggtgactaag atgttgtttg atcaaattgc
     7981 aagcaataaa cgaaaaacct ggattttgtt gctggtattt ttcctactct tagctcttgt
     8041 tggttatgcg gttggttatc tctttataag atctggactt ggtggtttgg ttatcgcact
     8101 gattatcggc tttatctacg ctttgtctat gatttttcaa tcgacagaga ttgtcatgtc
     8161 catgaatgga gcgcgtgagg tggatgagca aacggcacca gacctctacc atgtagtgga
     8221 agatatggct ctggtcgctc agattcctat gccccgtatt ttcattattg atgatccagc
     8281 cttaaatgcc tttgcgacag gttctaatcc tcaaaatgcg gctgttgctg cgacttcagg
     8341 tctactagct atcatgaatc gtgaagaact agaagctgtt atgggacatg aagtcagtca
     8401 tattcgtaat tatgatatcc gtatttcgac tattgcagtt gcccttgcta gtgctatcac
     8461 catgctttct agtatggcag gtcgtatgat gtggtggggt ggagcaggtc gcagacgaag
     8521 tgatgatgac cgagatggaa atggtcttga aatcattatg ctagtggttt ccctactagc
     8581 tattgtactg gcacctctcg ctgcaacctt ggttcagctc gctatttctc gtcagaggga
     8641 atttctggca gatgcatcta gtgtcgagct gactcgcaat ccccagggaa tgattaatgc
     8701 cctagataag ttggacaata gcaaacctat gagtcgccac gtcgatgatg ctagcagtgc
     8761 cctttatatc aatgatccta agaaaggtgg ggggttccaa aaactctttt atacccaccc
     8821 acctatctca gaacggattg aacgtttaaa acagatgtaa aagaatccag aggtcgtctc
     8881 tggatttttg ctatgttgca aatttataaa tcttctaaat taactagctt caaaccgtgt
     8941 tctgtcatgc gatagatgat cgagcagact tcttttaata aacgacggtc atgcgaaaca
     9001 gtgatgagac cgcctggata ggtagcaaag agttttctga tttggggttg agaagtggga
     9061 gaaaagtttc gtgtgggttc atccagcagg agaaagtttg gtttgcgcag gactaaatcc
     9121 aaaagcagga gttttccctg ttgtccgcca gataaggagc gaatttgatg ctgcatttct
     9181 ggataactga aattgagact agctaggtga gattggattt tctgtagttc ctctttttcc
     9241 ccagttttac tgagataggc tattggggat aaatccaatt gcagtttttt gtggtaatct
     9301 tgtggcataa aaccaagtga aatctctctt ttatcattca gaagtctctg taacttggct
     9361 aacagagttg atttcccaac accatttggc ccgataatac cgattttttc ttggccacgg
     9421 acagttagtt gtagtttttg aaccaaaact cggtcgtcaa tggacaaatt ttctttttcc
     9481 agttggacta agactttaga agctggtaat ggttggatgt ctgaaaagaa aagttggatt
     9541 tgttcctctt caagtggctt ttgagtcatg gactgagctg ccttttcgta gcgtttttct
     9601 tgtgagagga cagttttcat ctttttagcc aataggcgac cggcagtact atctttggta
     9661 gctcgaagcg cagtttctac attttgctta actctccgat gtttttccat ggttttatcg
     9721 taggctcttt ggttgttagc agcttgctga ctttgtttgg caaaattagc ctttctctgc
     9781 tcactatagc tatcatagtc taaatgctct actagcgttt ccgcttcttt acggtgtttg
     9841 accagtcgca agtgaacaat agtgtctgcc gtttcagaaa gaaagtcttc atcatgggaa
     9901 atgaaaataa cggtttgcct ggtcttttga atctggcctt ttagccaatc aactgtctca
     9961 aggtctaggt catttgaagg ttcatctaaa aatagaatct caaagggttt ggctaactca
    10021 tggataagct gaattttcaa agcttcgccc cctgatagat tgccaatctc ttggtcactt
    10081 gcgaaacgat tgctatcaaa atgcaattcc tccgccaaac gatagaggat actgtagtct
    10141 aaatcaatag aatctaaaaa gaagtagtcg tgtaaagttt tcttttttag gtcctcaggg
    10201 actttttgag gaatgtaggc cagtgactga tagtcagatt ggatgtttcc cttgatagtg
    10261 aaatcagaca aagcttcccc cattaaaatt ttaagtaagg ttgatttgcc atttccttct
    10321 tcaccaataa tagcaacctt ttccccgtct tggatggtca tggttaggtc agatacaaga
    10381 tctcgtaaat ctttgttttg tgtgatggtt agatgattga tttttatcat atgatttccc
    10441 tttctagaat gttcatagtg cataacaaaa agctctactt tacctagtag agcccaaagt
    10501 ctatgtcaaa aaaactctat taacatgcca gaaaaagagc ataaaagagc acacaaaaag
    10561 agacaaaaac aagatctact aaataaaggt tctttatttg atagacttag ttgttcatgt
    10621 ctcccttacc tccgagtatt agtatcttta tattatacct ttaaggaaat tttgtcaaca
    10681 gaaaactgga aattctatgc tctaaaattc aaaaacaggt ccatagagac ttagtacgtg
    10741 tttgacatga tcgtaatgga atttatcata gtttcgccaa gcagtgcgtg cttgatcgtt
    10801 tagttttagg ctacccagtc caatcagaag actggtaggt tggtaaaatt tctcaagatc
    10861 aattaagata ctgttgttca actgttccgt ttttttgagc tcttgcatag tagtgtttag
    10921 cagctcttgt agtcgaatat catctgccgt accctgttta cagttttggt agcttttatt
    10981 taacttggat aggagttgaa tgagtttctt gtcttccatg tgagcatcct ccttgatctg
    11041 atgtactctt tccctatagt atagcactaa aaaataaaac tatcatagta aatatgttaa
    11101 aattttgaat tttaattaca aagtttggtg tgctgatttg tagccttttc atggtataat
    11161 caagagagta aatctttatg gaggaagtat gaaattaaat attcaagaaa ttcgtaagca
    11221 gtctgaaggt ttgaactttg aacaaacgtt agatttagtt gatgacctgc gtgcacgtaa
    11281 tcaagaaatt ttagatgtaa aagatatcct tgcagttggg aaagtacaat atgaagaccg
    11341 tatgtatttc ttagattatc aactatctta taccattgtt cttgcttcga gtcgcagtat
    11401 ggagccagtt gagttagttg aatcttatcc agtcacggaa gttttcatgg aaggcgcaac
    11461 taaccagcta gatcaagaag ttttagatga tgacttggtc ttgcccatcg aaaatgggga
    11521 gcttgacctt gctgagagtg tatcagacaa tatcctgcta aacattccta tcaaggtctt
    11581 gacggctgaa gaagaagctg gtcaaggatt tatctcagga aatgactggc aaatcatgac
    11641 agaggaagaa taccaagctc aaaaagcagt aaagaaagaa gaaaacagtc cttttgctgg
    11701 cttacaagga ctatttgacg gagatgaata atggtcttgt caaaaaaacg agcacgaaag
    11761 gtgctagaag aaatcattgc cctcttccca gatgccaagc ctagtcttga ttttaccaat
    11821 cattttgaac tcctggttgc ggtcatgtta tcagcccaga cgacagatgc agcggtaaat
    11881 aaggccacac caggtctctt tgttgccttt ccaacacccc aagctatgtc tgtagcgaca
    11941 gagagtgaga ttgcttcaca tatttctcgc ttgggattgt atcggaataa agctaaattc
    12001 cttaaaaaat gtgcccaaca gttactagac gattttgatg gtcaagtccc tcagacacgt
    12061 gaagaattgg agagtttggc aggtgttggt cgcaagacag ccaatgttgt catgagtgta
    12121 ggatttggaa ttccagcctt tgcagtggat actcatgtgg agcgtatttg caaacaccac
    12181 gatattgtca aaaaatcagc gacgccactt gaggtggaaa agcgggtcat ggatatcttg
    12241 ccgcctgagc agtggttagc tgcccatcag gccatgattt attttggaag agccatttgc
    12301 catccaaaaa atccagagtg tgaccagtac ccacaattat atgattttag caatttgtaa
    12361 gatgctacat tgaaactttt tgcttgattt taagcatgct ttgtgctata gtaaatgata
    12421 actaaatatt cctttaaacc tgtcccgtga ggcaggcaag gagcgcgata aagtagaagg
    12481 gtgaagtcta tactgtgtgc agtagggctc gcttggctat aaatttcaag tctccttgtt
    12541 taaggagact tttctttttg gaggtttgtc atgaaaacaa aagaagttgt agacgaattg
    12601 accgtcaaac gagcgattac gcgtattact tatgagatta tcgaacgcaa caaagatttg
    12661 aataagatcg tcttggctgg tattaaaact cgtggtgtct ttattgccca ccgaatccaa
    12721 gaacgtttga agcagttaga aaatctttca gttcctgttg tggaattgga tactaaacct
    12781 ttccgtgatg atgttaaaag tggagaagat acttctttgg tttctgtcga tgtgacagac
    12841 cgcgaagtta tcttggtgga tgatgtgctc tatacaggtc gtaccatccg tgctgctatt
    12901 gataatattg tcggtcatgg tcgtcctgcg cgtgtgagtt tagcagttct agtcgatcgt
    12961 ggacatagag aattgccaat ccgtccagat tacgttggaa aaaatatccc aaccagtcgt
    13021 tctgaagaaa tcatcgtaga gatgacagaa cttgatgacc aagacagagt tctgattact
    13081 gaagaagctt agaaagttaa aggagtagcc atgtcagaaa atcaacaagc attgaaccat
    13141 gtggtgtcca tggaagacct cactgtcgat caagtaatga aattgatcaa gcgaggaatt
    13201 gagtttaaaa atggagccca gcttccctat gaagaccatc cgattgtttc caatctcttc
    13261 tttgaggatt ctacacggac acataagtcc tttgaagtcg cagagattaa acttggattg
    13321 gaacgacttg actttgatgt gaagactagc tcggttaata agggtgagac actttatgac
    13381 accattttga ctctgtctgc tttaggagtg gatgtctgtg tgattcgcca tccagaggtc
    13441 gactactata gagaattgat tgctagtcca acgattacga cttccatcat caatggtgga
    13501 gatggttcgg gccaacaccc tagccagagc ttgcttgatt tgatgaccat ttatgaggaa
    13561 tttggccact ttgagggtct taaagttgct attgcaggtg acttggacca ctcacgcgtt
    13621 gccaaatcca atatgcagat tttgaaacgc ttgggagctg aactcttttt cgctggacct
    13681 gaggaatgga gaagtcaaga gtttgcagac tatggacagt ttgtaaccat tgatgaaatc
    13741 attgatcagg ttgatgtcat gatgtttctc cgtgtgcaac acgaacgcca tgatagtgga
    13801 gctgtatttt caaaagaaga ctaccatgcc caacatggct tgactcaaga acgttacgat
    13861 cgtttgaaag aaacagcaat cctcatgcac ccagctccaa tcaatcgtga tgtagaaata
    13921 gcagaccact tggttgaagc accaaaatca cggattgtcc aacaaatgac caatggtgtc
    13981 tttgttcgaa tggcaatctt agaatccgta ctagcgagta gaaacgccaa ctaaaacaca
    14041 agacgttaaa agacgccagt gagcgtccac gagaggtgaa aatatgacaa aaagaattct
    14101 agtattagaa gatggcacag tttttgaagg caaggccttc ggagcagata ttgatgtaac
    14161 aggcgaaatc gtctttaata cagggatgac cggctaccaa gaatccatta cagaccagtc
    14221 ttataatgga caaatcttga cctttactta tcctttggta ggaaattatg gtattaatcg
    14281 tgatgattat gaatccatta ttccaacttg taagggagtc gttgtttttg aagaagcgcg
    14341 tagagctagc aactggcgca accaaatgac cttggatgaa tttttgaaag ccaagaaaat
    14401 tccagggatt tcaggaattg atacgcgtgc tcttaccaag attatccgta agcatggtac
    14461 tatgcgtgca accttgaccc atgttgggga cagtatggac catgtgacgg accagctcca
    14521 agcaacagtt ttaccgacag acaatatcaa acaggtttct actaaaacgt cttatccagc
    14581 tccaggagtt ggtttgagcg tggtgctagt ggactttggt ctcaagcact caatcctacg
    14641 tgaactttct aaacgcaact gtaacgtgac agttgttcct tattcgacaa cggctgaaga
    14701 aattctccat ctcaatcctg acggagttat gttgtcaaat ggtccaggta acccagaaga
    14761 cgttccacaa gcactcgaca tgattcgcgg tgtacaagga aaaattccaa tctttggtat
    14821 ttgtatggga caccaactct ttgcaatggc aaacggggct aagacctata agatgaagtt
    14881 tggccaccgt ggattcaacc atgcggtacg tgaaatcgca acaggacgag tagacttcac
    14941 cagccagaac catggttatg cagtcagccg cgaggatttg ccagagcact tgattattac
    15001 ccacgaagaa atcaatgaca agtcagtgga aggtgtgcgc cacagatacc aacctgcttt
    15061 ctctgttcaa taccacccag atgcagctcc tgggccacac gacgctagct acctatttga
    15121 cgagtttatc gagatgatgg aagtttttaa acaatcaaac taagaacgag taaaagctcg
    15181 tcggagaatt tatgtaactg aacacggacg gaaagctcag aatgtagaga aaccaacttg
    15241 gattgtcaat ccttccttga tttcctaaaa ttcaatcgct ttctattcgt cctttgtatc
    15301 ttatttaatg tcgccctgag aggcgacgaa tagaaaggag aagggtgctc cgtatttgct
    15361 gaactgaata cgggctacgg acggtgctaa aaagatagtt attcctagaa ctgacagttc
    15421 ttcgtcataa ctcctatttt agctttgtcc gcttgacacc cttaatatct tatatatgcc
    15481 taaacgtact gatattcaaa aaattatggt gattggttct ggtccgatta ttattggtca
    15541 ggctgctgag tttgactacg ctgggactca ggcttgcttg tcgttgaaag aggaaggtta
    15601 tgaggttgtt ttggttaact caaatcctgc aaccatcatg acggacaagg aaattgctga
    15661 caaggtttac attgaaccga ttacacttga gtttgtgaca cgtattcttc gtaaggaagg
    15721 tccagatgcc ttgctaccaa cactcggtgg tcagacaggt ctcaacatgg ccatggaatt
    15781 atctaaaaat ggtatcctag acgagcttgg cgttgaactt ctgggtacca aattatctgc
    15841 cattgaccaa gcggaggacc gtgacctctt taaacaattg atggaagagc ttgaacaacc
    15901 cattccagaa tctgaaattg ttaacacagt agaagaagca gttgcctttg cagcgacaat
    15961 tggctaccca gtcatcgttc gtccagcctt tacccttggt ggtactggtg gtggtatgtg
    16021 tgccaacgag aaagaattgc gtgaaatcac tgaaaatggt ttgaaattgt cacctgttac
    16081 ccaatgtttg attgagcgtt cgattgccgg tttcaaggaa attgaatacg aagtgatgcg
    16141 tgactcagct gacaatgcct tggttgtttg taacatggaa aactttgacc cagttgggat
    16201 tcacacaggg gattccattg tatttgcccc tgcgcaaacc atgtcagact atgaaaacca
    16261 aatgcttcgt gatgcgagct tgagcattat tcgtgccctc aagattgaag gtggatgtaa
    16321 tgttcagcta gctcttgatc ctaacagctt caagtattat gttatcgaag taaaccctcg
    16381 tgtatcacgt tcgtcagccc ttgcttctaa agcgacagga tatccgattg ctaagttggc
    16441 tgccaagatt gcagtaggtt tgaccttgga tgaggttata aacccagtta caggttcaac
    16501 ctatgccatg tttgaaccag cccttgacta cgtagttgct aagattccac gtttcccatt
    16561 tgacaagttt gaaaagggtg aacgccgtct cggtacacaa atgaaggcga ctggagaagt
    16621 catggcaatc ggtcgtaaca tcgaggaatc tcttctcaag gcctgccgct cccttgaaat
    16681 tggggtgcac cacaatgaaa tacctgaact tgcagcagtt tctgatgatg ccttgattga
    16741 aaaggttgtc aaagcccaag atgaccgtct cttctacgta tcagaagcca ttcgtcgtgg
    16801 ctacacacct gaagaaattg cagagcttac aaaaattgat atcttctatc ttgataaact
    16861 cttgcatatc tttgaaattg agcaagaatt gggtgctcat ccacaagatt tagaagtttt
    16921 gaaaacagcc aaacttaatg gtttttcaga ccgtaagatt gctgaactct ggggaacgac
    16981 tgatgataaa gttcgccaac ttcgcttgga aaacaagatt gttccagttt ataagatggt
    17041 agacacttgt gcggcagagt tcgactccga aacgccatat ttctattcaa cctatggttg
    17101 ggaaaatgaa tctatcagat ctgataaaga atctgtactt gtcctaggtt caggtccaat
    17161 ccgtatcggt caaggggttg agtttgacta cgcaaccgtt cactctgtta aggctatcca
    17221 ggcagctggt tatgaagcta tcatcatgaa ctcaaaccca gagaccgttt caacagactt
    17281 ctctgtatca gataagcttt actttgagcc attgacattc gaagatgtta tgaacgtcat
    17341 tgacttggaa caaccaaagg gagttatcgt tcagttcggt ggtcaaacag ccatcaacct
    17401 tgcggagcca ttggcaaaag caggtgtgac catccttggt acacaggttg ctgacctaga
    17461 tcgtgccgaa gaccgtgacc tcttcgagca agctctcaaa gaattggata ttccacagcc
    17521 accaggacaa acggctacca atgaagaaga agcagctctt gcagctcgca agattggctt
    17581 cccagttctt gtccgcccat cttatgtact tggtggacgt gccatggaaa tcgttgaaaa
    17641 cgaagaagac cttcgttctt acatgcgaac tgctgttaag gctagtccag accacccagt
    17701 tcttgtcgac tcttatatcg ttgggcaaga gtgcgaagtt gatgccattt cagatggtaa
    17761 aaatgtactc attcctggta tcatggagca tatcgaacgt gctggtgtcc actcaggtga
    17821 ctcaatggct gtttacccac cacaaacctt gtcgcaaaag gttcaggaga ccatcgcaga
    17881 ctacaccaaa cgcttagcaa tcggtcttca ctgccttgga atgatgaaca tccagtttgt
    17941 tatcaaggat gaaaaagtct acgttattga ggtcaatcca cgtgccagcc gtactgttcc
    18001 attcctatct aaggtaacca atattcctat ggctcaagta gcgaccaaac tcattcttgg
    18061 tcaaagttta tcagaacttg gctaccaaaa tggactttac cctgaaagca cccgtgttca
    18121 tatcaaggca cctgtcttct cctttaccaa actagctaag gtagacagct tgctcggtcc
    18181 tgaaatgaag tcaacaggtg aagttatggg ttctgatgct actttggaaa aagctctcta
    18241 taaagccttt gaagcttctt atctacactt gccaactttt ggtaatgtcg tctttaccat
    18301 cgctgatgat gccaaagaag aagccttgaa cttggctcgt cgtttccaaa acattggtta
    18361 tggaatcctt gcgacagaag ggacagcagc cttctttgct agtcatggct tgcaagccca
    18421 acctgttggt aagattggtg atgacgataa ggatatccca agttttgtac gcaaaggaag
    18481 aattcaagct atcattaaca cagtaggaac aaaacgaact gctgacgaag atggtgagca
    18541 aattcgtcgt tcagccattg aacacggggt gcccctcttc acagcgctag atacagctaa
    18601 tgccatgctt aaggtacttg aaagtcgtag ctttgttaca gaagcgattt aaatagaaaa
    18661 ataagatata gcagactgaa
//
LOCUS       DAVMAF010000032        18528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMAF010000032 DAVMAF010000000
VERSION     DAVMAF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.032"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            28..1164
                     /gene="dnaN"
                     /locus_tag="VYO97_001175"
     CDS             28..1164
                     /gene="dnaN"
                     /locus_tag="VYO97_001175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1753978.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1229..1423
                     /locus_tag="VYO97_001176"
     CDS             1229..1423
                     /locus_tag="VYO97_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1753979.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1507..2622
                     /gene="ychF"
                     /locus_tag="VYO97_001177"
     CDS             1507..2622
                     /gene="ychF"
                     /locus_tag="VYO97_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1753980.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2693..3262
                     /locus_tag="VYO97_001178"
     CDS             2693..3262
                     /locus_tag="VYO97_001178"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1753981.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDEYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3263..6772
                     /gene="mfd"
                     /locus_tag="VYO97_001179"
     CDS             3263..6772
                     /gene="mfd"
                     /locus_tag="VYO97_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1753982.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLMSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6830..7096
                     /locus_tag="VYO97_001180"
     CDS             6830..7096
                     /locus_tag="VYO97_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1753983.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7089..7457
                     /locus_tag="VYO97_001181"
     CDS             7089..7457
                     /locus_tag="VYO97_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1753984.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7577..8845
                     /locus_tag="VYO97_001182"
     CDS             7577..8845
                     /locus_tag="VYO97_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1753985.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGELTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8842..10119
                     /gene="tilS"
                     /locus_tag="VYO97_001183"
     CDS             8842..10119
                     /gene="tilS"
                     /locus_tag="VYO97_001183"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1753986.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10123..10665
                     /gene="hpt"
                     /locus_tag="VYO97_001184"
     CDS             10123..10665
                     /gene="hpt"
                     /locus_tag="VYO97_001184"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1753987.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10681..12639
                     /gene="ftsH"
                     /locus_tag="VYO97_001185"
     CDS             10681..12639
                     /gene="ftsH"
                     /locus_tag="VYO97_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1753988.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12761..13240
                     /locus_tag="VYO97_001186"
     CDS             12761..13240
                     /locus_tag="VYO97_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1753989.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            13334..13405
                     /locus_tag="VYO97_001187"
     tRNA            13334..13405
                     /locus_tag="VYO97_001187"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13367..13369,aa:Glu,seq:ttc)
     gene            13658..15203
                     /locus_tag="VYO97_001188"
     rRNA            13658..15203
                     /locus_tag="VYO97_001188"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            15255..15327
                     /locus_tag="VYO97_001189"
     tRNA            15255..15327
                     /locus_tag="VYO97_001189"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15288..15290,aa:Ala,seq:tgc)
     gene            15452..18353
                     /locus_tag="VYO97_001190"
     rRNA            15452..18353
                     /locus_tag="VYO97_001190"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            18430..>18528
                     /gene="rrf"
                     /locus_tag="VYO97_001191"
     rRNA            18430..>18528
                     /gene="rrf"
                     /locus_tag="VYO97_001191"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6092 a         3042 c         4247 g         5147 t
ORIGIN      
        1 atactaaaaa taaataaagg agaatccatg attcattttt caattaataa aaatttattt
       61 ctacaagcat taaatactac taagagagct attagttcta aaaatgccat tcctatttta
      121 tcaacagtaa aaattgacgt gaccaatgaa ggtattactt taattggttc aaatggtcaa
      181 atttcaattg aaaattttat ttctcaaaaa aatgaagatg ctggtttgtt aattacttct
      241 ttaggttcga tccttcttga agcttctttc tttatcaatg tagtatctag tttacctgat
      301 gtaactcttg attttaaaga aattgaacaa aatcaaattg ttttaaccag tggcaaatca
      361 gaaattaccc taaaaggaaa agatagcgaa caatatccac gaatccaaga aatttcagca
      421 agcactcctt taatacttga aacaaaatta ctcaagaaaa ttattaatga aacagccttt
      481 gctgcaagta cacaagagag tcgtccgatt ttaacaggtg tccacttcgt attgagtcaa
      541 cacaaagagt taaaaacagt tgcaacagac tctcatcgcc taagccagaa aaaattgact
      601 cttgaaaaaa atagtgatga ttttgatgtc gtaattccta gccgttctct acgcgaattt
      661 tcagcggtat ttacagatga tatcgaaact gtagagattt tctttgccaa taaccaaatc
      721 ctctttagaa gcgaaaatat tagcttctat actcgtctcc tagaaggaaa ctatcctgat
      781 acagatcgct tgattccaac agactttaac actactatta cttttaatgt ggtaaactta
      841 cgccagtcaa tggagcgtgc ccgtctttta tcaagtgcga ctcaaaatgg tactgtgaaa
      901 cttgaaatta aggatggggt tgttagcgcc catgttcact ctccagaagt tggtaaagta
      961 aacgaagaaa tcgatactga tcaggttact ggtgaagatt tgaccattag tttcaaccca
     1021 acttacttga ttgattctct taaagcttta aatagcgaaa aggtgaccat tagctttatc
     1081 tcagctgttc gtccatttac tcttgtgcca gcagatactg acgaagactt catgcagctc
     1141 attacaccag ttcgtacaaa ttaagtgaaa gaggttgagc ctggctcgcc tcttttatga
     1201 tataatcgaa aaagaaaagg agagtagtat gtatcaagtt ggaaattttg ttgagatgaa
     1261 aaaatcacac gcttgtacaa tcaagtcgac tggtaaaaag gctaatcgtt gggaaattac
     1321 acgtgtagga gcagatatca aaataaaatg cagtaattgt gagcatgttg tcatgatggg
     1381 gcgatatgat tttgagcgaa aaatgaataa aattattgac tgagaaccct tagttagagg
     1441 gttagcactt tatccctttt tgtgttataa tattagggat tgaaatgaaa acggagaatg
     1501 agaaatatgg ctttgacagc aggtatcgtt ggtttgccaa acgttggtaa atcaacacta
     1561 tttaatgcaa ttacaaaagc aggagcagag gcagcaaact acccatttgc gactattgat
     1621 ccaaatgttg gaatggtgga agttccagat gaacgcctac aaaaactaac tgaaatgata
     1681 actcctaaaa agacagttcc cacaacattt gaatttacgg atattgcagg gattgtaaaa
     1741 ggagcttcaa aaggagaagg gctagggaat aaattcttgg ccaatattcg tgaagtagat
     1801 gcgattgttc acgtagttcg tgcttttgat gatgaaaatg tgatgcgcga gcaaggacgt
     1861 gaagacgcct ttgtagatcc acttgcagat attgatacaa ttaatctgga attaattctt
     1921 gctgacttag aatcagtgaa caaacgatat gcgcgtgtag aaaagatggc acgtacgcaa
     1981 aaagataaag aatcagtagc agaattcaat gttcttcaaa agattaaacc agtcctagaa
     2041 gacgggaaat cagctcgtac cattgaattt acagatgagg aacaaaaggt tgtcaaaggt
     2101 cttttccttt tgacgactaa accagttctt tatgtagcta atgtggacga ggatgtggtt
     2161 tcagaacctg actctatcga ctatgtcaaa caaattcgtg aatttgcagc gacagaaaat
     2221 gctgaagtag tcgttatttc tgcgcgtgct gaggaagaaa tttctgaatt ggatgatgaa
     2281 gataaaaaag agtttcttga agccattggt ttgacagaat caggtgtaga taagttgacg
     2341 cgtgcagctt accacttgct tggattggga acttacttca cagctggtga aaaagaagtt
     2401 cgcgcttgga ctttcaaacg tggtatgaag gctcctcaag cagctggtat tatccactca
     2461 gactttgaaa aaggctttat tcgtgcagta accatgtcat atgaagatct agtgaaatac
     2521 ggatctgaaa aggccgtaaa agaagctgga cgcttgcgtg aagaaggaaa agaatatatc
     2581 gttcaagatg gcgatatcat ggaattccgc tttaatgtct aaaaattaat aaatggtgtc
     2641 aattaggttg gaaaaaaatt ccaacccttt tggcttttga aaggaaaaat aaatgaccaa
     2701 attacttgta ggcttgggaa atccagggga taaatatttt gaaacaaaac acaatgttgg
     2761 ttttatgttg attgatcaac tagcgaagaa acagaatgtc acttttacac acgataagat
     2821 atttcaagct gacctagcat cctttttcct aaatggagaa aaaatttatc tggttaaacc
     2881 aacgaccttt atgaatgaaa gtggaaaagc agttcatgct ttattaactt actatggttt
     2941 ggatattgac gatttactta tcatttacga tgatcttgac atggaagttg ggaaaattcg
     3001 tttaagagca aaaggctcag caggtggtca taatggtatc aagtctatta ttcaacatat
     3061 aggaactcag gtctttaacc gtgttaagat tggaattgga agacctaaaa atggtatgtc
     3121 agttgttcat catgttttga gtaagtttga cagggatgag tatatcggta ttttacagtc
     3181 tgttgacaaa gttgacgatt ctgtaaacta ctatttacaa gagaaaaatt ttgagaaaac
     3241 aatgcagagg tataacggat aaatggtgac cttattagat ttattctcag aaaatgatca
     3301 gattaaaaaa tggcatcaaa atttaacaga taagaaaaga caactaatac ttggtttatc
     3361 aacatctact aaggctcttg caattgcaag cagtttagaa aaagaagata ggattgtgtt
     3421 attgatgtca acttatggag aagcagaagg acttgttagt gatcttatat ctatcttggg
     3481 tgaggaactc gtctatccat ttttggtaga tgatgctcct atggtggagt ttttgatgtc
     3541 ttcacaggaa aaaattattt cacgggttga agccttgcgt tttttgactg attcatctaa
     3601 gaaagggatt ttagtttgta atatcgcagc aagtcgattg attttaccgt ctcccaatgc
     3661 attcaaagat agtattgtaa aaatctcagt tggtgaagaa tatgatcaac acgcgtttat
     3721 ccatcagtta aaggaaaatg gctatcgaaa agttactcaa gtacaaactc agggcgaatt
     3781 tagtcttcga ggagatattt tagatatttt tgaaatatcc cagttagaac cttgtcgaat
     3841 tgagtttttt ggtgatgaaa ttgatggtat caggtcattt gaagtagaaa cacaattatc
     3901 gaaagaaaat aagacagaac tcactatctt tccagctagt gatatgcttt tgagagaaaa
     3961 ggattatcaa cgaggacagt cagctttaga aaaacaaatt tcaaaaactt tatcacctat
     4021 tttgaaatca tacctagaag aaattctttc aagttttcac caaaaacaaa gtcatgcaga
     4081 ctctcggaag tttttatctt tgtgctatga taagacatgg actgtctttg attatattga
     4141 aaaagatact ccaatattct ttgatgatta tcaaaaattg atgaatcagt atgaagtctt
     4201 tgaaagagaa ttagcgcagt actttacaga agaattacag aatagtaaag cattttctga
     4261 tatgcagtat ttttctgata ttgaacaaat ctataaaaaa caaagtccag tgaccttttt
     4321 ttctaatctt caaaagggtt taggaaatct caaatttgac aaaatttatc aattcaatca
     4381 atatcctatg caggaatttt tcaatcagtt ttcttttcta aaagaagaaa ttgaacgata
     4441 taaaaaaatg gattacacca ttattctgca gtctagcaat tcaatgggaa gtaaaacatt
     4501 ggaggatatg ttagaggaat atcagattaa attggattct agagataaga caaatatctg
     4561 taaagaatct gtaaacttaa tagagggtaa tctcagacat ggttttcatt ttgtagatga
     4621 aaagatttta ttgataactg aacatgagat ttttcaaaag aaattaaagc gtcgttttcg
     4681 aagacaacat gtttcaaatg cagagagatt aaaagattac aatgaacttg aaaaagggga
     4741 ctatgttgtc catcatatcc atgggattgg tcaatatcta ggaattgaaa ccattgaaat
     4801 caagggaatt catcgtgatt atgtcagtgt ccaataccaa aatggtgatc aaatttctat
     4861 tcccgtggaa cagattcatc tactgtccaa atatatttca agtgatggta aagctccaaa
     4921 actcaataaa ttaaatgacg gtcattttaa aaaggccaag caaaaggtta agaaccaggt
     4981 agaggatata gctgatgatt taatcaaact ctactctgaa cgtagtcagt tgaagggttt
     5041 tgctttctca gctgatgatg atgatcaaga tgcctttgat gatgctttcc cttatgttga
     5101 aacggatgat caacttcgta gtattgagga aatcaagagg gatatgcagg cttctcagcc
     5161 aatggatcga cttttagttg gggatgttgg ttttggaaag actgaagttg ctatgcgtgc
     5221 agcctttaaa gcagtcaatg atcacaaaca ggttgtcatt ctagttccga cgacggtttt
     5281 agcgcaacag cactatacga attttaagga acgattccaa aattttgcag ttaatattga
     5341 tgtgttgagt cgctttagaa gtaaaaaaga gcagactgca acacttgaaa aattgaaaaa
     5401 cggtcaagtc gatattttga ttggaacaca tcgtgttttg tcaaaagatg ttgtgtttgc
     5461 tgatttgggc ttgatgatta ttgatgagga acagcgattt ggtgtcaagc ataaggaaac
     5521 tttgaaagaa ctgaagaaac aagtggatgt cctaaccttg accgctacgc caatccctcg
     5581 taccctccat atgtctatgc tgggaatcag agatttatct gttattgaaa ctccgccgac
     5641 taatcgctat cctgttcaga cctatgtttt ggaaaagaat gatagtgtca ttcgtgatgc
     5701 tgtcttgcgt gaaatggagc gtggaggtca agtttattat ctttacaaca aagttgacac
     5761 aattgttcag aaggtttcag aattacagga gttgattccg gaggcttcga ttggatatgt
     5821 tcatggtcga atgagtgaag tccagttgga aaatactcta ttagacttta ttgagggaca
     5881 atacgatatc ttggtgacga ctactattat tgagacaggg gtggacattc caaatgctaa
     5941 tactttattt attgaaaatg cggaccatat gggcttgtca accttatatc agttaagagg
     6001 aagagtcggt cgtagtaatc gtattgctta tgcttatctc atgtatcgtc cagaaaaatc
     6061 aatcagtgaa gtctctgaaa agagattaga agcgattaaa ggatttacag aattgggctc
     6121 tggctttaag attgcaatgc gagatctttc gattcgtgga gcaggaaatc ttttaggaaa
     6181 atcccagtct ggtttcattg attctgttgg ttttgaattg tattcgcagt tattagagga
     6241 agctattgct aaacgaaacg gtaatgctaa cgctaacaca agaaccaaag ggaatgctga
     6301 gttgattttg caaattgatg cctatcttcc tgatacttat atttctgatc aacgacataa
     6361 gattgaaatt tacaagaaaa ttcgtcaaat tgacaaccgt gtcaattatg aagagttaca
     6421 agaggagttg atagaccgtt ttggagaata cccagatgta gtagcctatc ttttagagat
     6481 tggtttggtc aaatcatact tggacaaggt ctttgttcaa cgtgtggaaa gaaaagataa
     6541 taaaattaca attcaatttg aaaaagtcac tcaacgactg tttttagctc aagattattt
     6601 taaagcttta tccgtaacga acttaaaagc aggcatcgct gagaataagg aattaatgga
     6661 gcttgtattt gatgttcaaa ataagaaaga ttatgaaatt ttagaaggtc tgctgatttt
     6721 tggagaaagt ttattagaga taaaagagtc taaggaagaa aattccattt gatatttttc
     6781 ttctataaaa tagataaaaa tggtacaata ataaattgag gtaataagga tgagattaga
     6841 taaatattta aaagtatcgc gaattatcaa gcgtcgtaca gtcgcaaagg aagtagcaga
     6901 taaaggtaga atcaaggtta atggaatctt ggccaaaagt tcaacggact tgaaagttaa
     6961 tgaccaagtt gaaattcgct ttggcaataa gttgctgctt gtaaaagtac tagagatgaa
     7021 agatagtaca aaaaaagaag atgcagcagg aatgtatgaa attatcagtg aaacacgggt
     7081 agaagaaaat gtctaaaaat attgtacaat tgaataattc ttttattcaa aatgaatacc
     7141 aacgtcgtcg ctacctgatg aaagaacgac aaaaacggaa tcgttttatg ggaggggtat
     7201 tgattttgat tatgctatta tttatcttgc caacttttaa tttagcgcag agttatcagc
     7261 aattactcca aagacgtcag caattagcag acttgcaaac tcagtatcaa actttgagtg
     7321 atgaaaagga taaggagaca gcatttgcta ccaagttgaa agatgaagat tatgctgcta
     7381 aatatacacg agcgaagtac tattattcta agtcgaggga aaaagtttat acgattcctg
     7441 acttgcttca aaggtgataa aatggaaaat ttattagacg taatagagca atttttgggt
     7501 ttgtcagatg aaaagttgga agaattggct gataaaaatc aattattgcg tttacaagaa
     7561 gaaaaggaaa ggaagaatgc gtaaattctt aattattttg ttgctaccaa gttttttgac
     7621 catttcaaaa gtcgttagca cagaaaaaga agtcgtctat acttcgaaag aaatttatta
     7681 cctttcacaa tctgactttg gtatttattt tagagaaaaa ttaagttctc ccatggttta
     7741 tggagaggtt cctgtttatg cgaatgaaga tttagtagtg gaatctgggg aattgactcc
     7801 caaaacaagt tttcaaataa ccgagtggcg cttaaataaa caagggattc cagtatttaa
     7861 gctatcaaat catcaattta tagctgcgga caaacgattt ttatatgatc aatcagaggt
     7921 aactccaaca ataaaaaaag tatggttaga atctgacttt aaactgtaca atagtcctta
     7981 tgatttaaaa gaagtgaaat catccttatc agcttattcg caagtatcaa tcgacaagac
     8041 catgtttgta gaaggaagag aatttctaca tattgatcag gctggatggg tagctaaaga
     8101 atcaacttct gaagaagata atcggatgag taaagttcaa gaaatgttat ctgaaaaata
     8161 tcagaaagat tctttctcta tttatgttaa gcaactgact actggaaaag aagctggtat
     8221 caatcaagat gaaaagatgt atgcagccag cgttttgaaa ctctcctatc tctattatac
     8281 gcaagaaaaa ataaatgagg gtctttatca gttagatacg actgtaaaat acgtatctgc
     8341 agtcaatgat tttccaggtt cttataaacc agagggaagt ggtagtcttc ctaaaaaaga
     8401 agataataaa gaatattctt taaaggattt aattacgaaa gtatcaaaag aatctgataa
     8461 tgtagctcat aatctattgg gatattacat ttcaaaccaa tctgatgcca cattcaaatc
     8521 caaaatgtct gccattatgg gagatgattg ggatccaaaa gaaaaattga tttcttctaa
     8581 gatggccggg aagtttatgg aagctattta taatcaaaat ggatttgtgc tagagtcttt
     8641 gactaaaaca gattttgata gtcagcgaat tgccaaaggt gtttctgtta aagtagctca
     8701 taaaattgga gatgcggatg aatttaagca tgatacgggt gttgtctatg cagattctcc
     8761 atttattctt tctattttca ctaagaattc tgattatgat acgatttcta agatagccaa
     8821 ggatgtttat gaggttctaa aatgagggaa ccagattttt taaatcattt tctcaagaag
     8881 ggatatttca aaaagcatgc taaggcggtt ctagctcttt ctggtggatt agattctatg
     8941 tttctattta aggtattgtc tacttatcaa aaagagttag agattgaatt gattctggct
     9001 catgtgaatc ataagcagag aattgaatca gattgggaag aaaaggaatt aaggaagttg
     9061 gctgctgaag cagagcttcc tatttatatc agcaattttt caggagaatt ttcagaagcg
     9121 cgtgcacgaa attttcgtta tgattttttt caagaggtta tgaaaaagac aggtgcgaca
     9181 gctttagtca ctgcccacca tgctgatgat caggtggaaa cgattttgat gcgcttgatt
     9241 cgaggaactc gcttgcgcta tctatcagga attaaggaga agcaagtagt cggagagata
     9301 gaaatcattc gtcccttctt gcattttcag aaaaaagact ttccatcaat ttttcacttt
     9361 gaagatacat caaatcagga gaatcattat tttcgaaatc gtattcgaaa ctcttactta
     9421 ccagaattgg aaaaagaaaa tcctcgattt agggatgcaa tcttaggcat tggcaatgaa
     9481 attttagatt atgatttggc aatagctgaa ttatctaaca atattaatgt ggaagattta
     9541 cagcagttat tttcttactc tgagtctaca caaagagttt tacttcaaac ttatctgaat
     9601 cgttttccag atttgaatct tacaaaagct cagtttgctg aagttcagca gattttaaaa
     9661 tctaaaagcc agtatcgtca tccgattaaa aatggctatg aattgataaa agagtaccaa
     9721 cagtttcaga tttgtaaaat cagtccgcag gctgatgaaa aggaagatga acttgtgtta
     9781 cactatcaaa atcaggtagc ttatcaagga tatttatttt cctttggact tccattagaa
     9841 ggtgaatcaa ttcaacaaat acctgtttca cgtgaaacat ccatacacat tcgtcatcga
     9901 aaaacaggag atgttttgat tcaaaatggg catagaaaaa aactcagacg tttatttatt
     9961 gatttgaaaa tccctatgga aaagagaaac tctgctctta ttattgagca atttggtgaa
    10021 attgtctcaa ttttgggaat tgcgaccaat aatttgagta aaaaaacgaa aaatgatata
    10081 atgaacactg tactttatat agaaaaaata gataggtaaa aaatgttaga aaacgatatt
    10141 aaaaaagtcc tcgtttcaca cgatgaaatt acagaagcag ctaaaaaact aggtgctcaa
    10201 ttaactaaag attatgcagg aaaaaatcca atcttagttg ggattttaaa aggatctatt
    10261 ccttttatgg ctgaattggt caaacatatt gatacacata ttgaaatgga cttcatgatg
    10321 gtttctagct accatggtgg aacagcaagt agtggtgtta tcaatattaa acaagatgtg
    10381 actcaagata tcaaaggaag acatgttcta tttgtagaag atatcattga tacaggtcaa
    10441 actttgaaga atttgcgaga tatgtttaaa gcaagagaag cagcttctgt taaaattgca
    10501 accttgttgg ataaaccaga aggacgtgtt gtagaaattg aggcagacta tacctgcttt
    10561 actatcccaa atgagtttgt agtaggttat ggtttagact acaaagaaaa ttatcgtaat
    10621 cttccttata ttggagtatt gaaagaggaa gtgtattcaa attagaaaga ataatcttta
    10681 atgaaaaaac aaaataatgg tttaattaaa aatccttttc tatggttatt atttatcttt
    10741 ttccttgtga caggattcca gtatttctat tctgggaata actcaggagg aagtcagcaa
    10801 atcaactata ctgagttggt acaagaaatt accgatggta atgtaaaaga attaacttac
    10861 caaccaaatg gtagtgttat cgaagtttct ggtgtctata aaaatcctaa aacaagtaaa
    10921 gaaggaacag gtattcagtt tttcacgcca tctgttacta aggtagagaa atttaccagc
    10981 actattcttc ctgcagatac taccgtatca gaattgcaaa aacttgctac tgaccataaa
    11041 gcagaagtaa ctgttaagca tgaaagttca agtggtatat ggattaatct actcgtatcc
    11101 attgtgccat ttggaattct attcttcttc ctattctcta tgatgggaaa tatgggagga
    11161 ggcaatggcc gtaatccaat gagttttgga cgtagtaagg ctaaagcagc aaataaagaa
    11221 gatattaaag taagattttc agatgttgct ggagctgagg aagaaaaaca agaactagtt
    11281 gaagttgttg agttcttaaa agatccaaaa cgattcacaa aacttggagc ccgtattcca
    11341 gcaggtgttc ttttggaggg acctccgggg acaggtaaga ctttgcttgc taaggcagtc
    11401 gctggagaag caggtgttcc attctttagt atctcaggtt ctgactttgt agaaatgttt
    11461 gtcggagttg gagctagtcg tgttcgctct ctttttgagg atgccaaaaa agcagcacca
    11521 gctatcatct ttatcgatga aattgatgct gttggacgtc aacgtggagt cggtctcggc
    11581 ggaggtaatg acgaacgtga acaaaccttg aaccaacttt tgattgagat ggatggtttt
    11641 gagggaaatg aagggattat cgtcatcgct gcgacaaacc gttcagatgt acttgatcct
    11701 gcccttttgc gtccaggacg ttttgataga aaagtattgg ttggccgtcc tgatgttaaa
    11761 ggtcgtgaag caatcttgaa agttcacgct aagaacaagc ctttagcaga agatgttgat
    11821 ttgaaattag tggctcaaca aactccaggc tttgttggtg ctgatttaga gaatgtcttg
    11881 aatgaagcag ctttagttgc tgctcgtcgc aataaatcga taattgatgc ttcagatatt
    11941 gatgaagcag aagatagagt tattgctgga ccttctaaga aagataagac agtttcacaa
    12001 aaagaacgag aattggttgc ttaccatgag gcaggacata ccattgttgg tctagtcttg
    12061 tcgaatgctc gcgttgtcca taaggttaca attgtaccac gcggccgtgc aggcggatac
    12121 atgattgcac ttcctaaaga ggatcaaatg cttctatcta aagaagatat gaaagagcaa
    12181 ttggctggct taatgggtgg acgtgtagct gaagaaatta tctttaatgt ccaaactaca
    12241 ggagcttcaa acgactttga acaagcgaca caaatggcac gtgcaatggt tacagagtac
    12301 ggtatgagtg aaaaacttgg cccagtacaa tatgaaggaa accatgctat gcttggtgca
    12361 cagagtcctc aaaaatcaat ttcagaacaa acagcttatg aaattgatga agaggttcgt
    12421 tcattattaa atgaggcacg aaataaagct gctgaaatta ttcagtcaaa tcgtgaaact
    12481 cacaagttaa ttgcagaagc attattgaaa tacgaaacat tggatagtac acaaattaaa
    12541 gctctttacg aaacaggaaa gatgcctgaa gcagtagaag aggaatctca tgcactatcc
    12601 tatgatgaag taaagtcaaa aatgaatgac gaaaaataac cctgagagag gctggagcct
    12661 ctcttttttg tacagtttag gagctaaagg gaacagaatg gataaaatgg aacaaatgtg
    12721 ttttctaatc tgttagactg tatctagaaa ggggaaaatt atgattaaag aattgtatga
    12781 agaagtccaa gggactgtgt ataagtgtag aaatgaatat taccttcatt tatgggaatt
    12841 gtcggattgg gaccaagaag gcatgctctg cttacatgaa ttgattagta gagaagaagg
    12901 actggcagac gatattccac gtttaaggaa atatttcaaa accaagtttc gaaatcgaat
    12961 tttagactat atccgtaagc aggaaagtca gaagcgtaga tacgataaag aaccctatga
    13021 agaagtgggt gagatcagtc atcgtataag tgagggaggt ctctggctag atgattatta
    13081 tctctttcat gaaacactaa gagattatag aaacaaacaa agtaaagaga aacaagaaga
    13141 actagaacgc gtcttaagca atgaacgatt tcgagggcgt caaagagtat taagagactt
    13201 acgcattgtg tttaaggagt ttactatccg tacccactag taagtcatgc aaaaaaaatg
    13261 aaaaaaatta gaaaaagtag ttgacaaagt ttgaaaagac tgtataatag taagagttga
    13321 aaataacaac tcaggtccgt tggtcaaggg gttaagacac cgccttttca cggcggtaac
    13381 acgggttcga atcccgtacg gactatggta tgttgcgtca ggaccacttg atgaaaaaaa
    13441 gtttaaaaaa acttaaaaat cttcaaaaaa gtgttgacaa gtgaaagtgg ctgtgatata
    13501 ctaatatagt tgtcgcttga gagaagcaag tgacaaagac ctttgaaaac tgaacaagac
    13561 gaaccaatgt gcagggcgct acaacataag ttgtagtact gaacaatgaa aaaaacaata
    13621 aatctgtcag tgacagaaat gagtgagaac tcaaactttt aatgagagtt tgatcctggc
    13681 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgaa ggaggagctt
    13741 gcttctctgg atgagttgcg aacgggtgag taacgcgtag gtaacctgcc tggtagcggg
    13801 ggataactat tggaaacgat agctaatacc gcataagagt agatgttgca tgacatttgc
    13861 ttaaaaggtg cacttgcatc actaccagat ggacctgcgt tgtattagct agttggtggg
    13921 gtaagggctc accaaggcga cgatacatag ccgacctgag agggtgatcg gccacactgg
    13981 gactgagaca cggcccagac tcctacggga ggcagcagta gggaatcttc ggcaatggac
    14041 ggaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt
    14101 tgtaagagaa gaacgagtgt gagagtggaa agttcacact gtgacggtat cttaccagaa
    14161 agggacggct aactacgtgc cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg
    14221 atttattggg cgtaaagcga gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc
    14281 ttaaccatag taggctttgg aaactgttta acttgagtgc aagaggggag agtggaattc
    14341 catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cggtggcgaa agcggctctc
    14401 tggcttgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg
    14461 gtagtccacg ctgtaaacga tgagtgctag gtgttagacc ctttccgggg tttagtgccg
    14521 tagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga
    14581 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa
    14641 ccttaccagg tcttgacatc cctctgaccg ctctagagat agagttttcc ttcgggacag
    14701 aggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc
    14761 gcaacgagcg caacccctat tgttagttgc catcatttag ttgggcactc tagcgagact
    14821 gccggtaata aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct
    14881 gggctacaca cgtgctacaa tggctggtac aacgagtcgc aagccgatga cggcaagcta
    14941 atctcttaaa gccagtctca gttcggattg taggctgcaa ctcgcctaca tgaagtcgga
    15001 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac
    15061 cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc
    15121 agccgcctaa ggtgggatag atgattgggg tgaagtcgta acaaggtagc cgtatcggaa
    15181 ggtgcggctg gatcacctcc tttctaagga taaggaactg cgcattggtc ttgtttagtc
    15241 ttgagaggtc ttgtggggcc ttagctcagc tgggagagcg cctgctttgc acgcaggagg
    15301 tcagcggttc gatcccgcta ggctccattg gtgagagatc accaagtaat gcacattgaa
    15361 aattgaatat ctatatcaaa tagtaacaag aaaataaacc gaaaacgctg tagtattaat
    15421 aagagtttat gactgaaagg tcagaaaata aggttaagtt aataagggcg cacggtggat
    15481 gccttggcac taggagccga cgaaggacgt gacaaacgac gatatgcctt gggtagctgt
    15541 aagtaagcga tgatccaggg atttccgaat gggggaaccc aacaggtaat acctgttacc
    15601 cacatctgtt aaggatgtga ggaggaagac gcagtgaact gaaacatcta agtagctgca
    15661 ggaagagaaa gcaaaagcga ttgccttagt agcggcgagc gaaacggcag aagggcaaac
    15721 cgaagagttt actcttcggg gttgtaggac tgcaatgtgg actcaaagat tatagaagaa
    15781 tgatttggga agatcagcca aagagagtaa tagcctcgta tttaaaatag tctttgtacc
    15841 tagcagtatc ctgagtacgg cgggacacgt gaaatcccgt cggaatctgg gaggaccatt
    15901 tcccaaccct aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga
    15961 aaagcacccc gggaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga
    16021 gcccgttaat gggtgagagc gtgccttttg tagaatgaac cggcgagtta cgttatgatg
    16081 cgaggttaag ttgaagagac ggagccgtag ggaaaccgag tctgaatagg gcgccttagt
    16141 atcatgacgt agacccgaaa ccatgtgacc tacccatgag caggttgaag gtgcggtaag
    16201 acgcactgga ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg
    16261 gagaaattcc aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc
    16321 gtcgacatta gagattcttg gaggtagagc actgtttggg tgaggggtcc atcccggatt
    16381 accaatctca gataaactcc gaatgccaat gaattatggt cggcagtcag actgcgagtg
    16441 ctaagatccg tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataatt
    16501 gttaagtgga aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct
    16561 attcattcaa agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc
    16621 ggggctaaaa caatttaccg aagctgtgga tacctttata ggtatggtag gagagcgttc
    16681 tatgtgtgat gaaggtatac cgtgaggagt gctggaacgc atagaagtga gaatgccggt
    16741 atgagtagcg aaagacaggt gagaatcctg tccaccgtaa gactaaggtt tccaggggaa
    16801 ggctcgtccg ccctgggtta gtcgggacct aaggagagac cgaaaggtgt atccgatgga
    16861 caacaggttg atattcctgt actagagtat gtagtgatgg agggacgcag taggctaact
    16921 aaagcagacg attggaagag tctgtctaag cagtgaggtg tgaattgagt caaatgctta
    16981 attctataac attgagctgt gatggggagc gaagtttagt agcgaagtta gtgacgtcac
    17041 actgccaaga aaagcttcta gcgtttaaac atactctacc cgtaccgcaa accgacacag
    17101 gtagtcgagg cgagtagcct caggtgagcg agagaactct cgttaaggaa ctcggcaaaa
    17161 tgaccccgta acttcgggag aaggggtgct gactttaagt cagccgcagt gaataggccc
    17221 aagcaactgt ttatcaaaaa cacagctctc tgctaaatcg taagatgatg tatagggggt
    17281 gacgcctgcc cggtgctgga aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt
    17341 gaagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
    17401 gggtaagttc cgacccgcac gaaaggcgta atgatttggg cactgtctca acgagagact
    17461 cggtgaaatt ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca
    17521 tggagcttta ctgcagtttg atattgagtg tctgtaccac atgtacagga taggtaggag
    17581 tctaagagat cgggacgcca gtttcgaagg agacgctgtt gggatactac ccttgtgtta
    17641 tggccactct aacccagata ggtgatccct atcggagaca gtgtctgacg ggcagtttga
    17701 ctggggcggt cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg
    17761 gaaatcattc gcagagtgta aaggtataag ggagcttgac tgcgagagct acaactcgag
    17821 cagggacgaa agtcgggctt agtgatccgg tggttccgta tggaagggcc atcgctcaac
    17881 ggataaaagc taccctgggg ataacaggct tatctccccc aagagttcac atcgacgggg
    17941 aggtttggca cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt
    18001 gggctgttcg cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg
    18061 gtccctatcc gtcgcgggcg taggaaattt gagaggatct gctcctagta cgagaggacc
    18121 agagtggact taccgctggt gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg
    18181 tagggaaggg ataaacgctg aaagcatcta agtgtgaaac ccacctcaag atgagatttc
    18241 ccatgattat atatcagtaa gagccctgag agatgatcag gtagataggt tagaagtgga
    18301 agtgtggcga cacatgtagc ggactaatac taatagctcg aggacttatc caaagtaact
    18361 gagaatatga aagcgaacgg ttttcttaaa ttgaatagat attcaatttt gagtaggtat
    18421 tactcagagt taagtgacga tagcctagga gatacacctg tacccatgcc gaacacagaa
    18481 gttaagccct agaacgccgg aagtagttgg gggttgcccc ctgtgaga
//
LOCUS       DAVMAF010000033        18004 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMAF010000033 DAVMAF010000000
VERSION     DAVMAF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.033"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            202..549
                     /gene="ssrA"
                     /locus_tag="VYO97_001192"
     tmRNA           202..549
                     /gene="ssrA"
                     /locus_tag="VYO97_001192"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(732..819)
                     /locus_tag="VYO97_001193"
     tRNA            complement(732..819)
                     /locus_tag="VYO97_001193"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Ser,seq:gga)
     gene            981..1721
                     /locus_tag="VYO97_001194"
     CDS             981..1721
                     /locus_tag="VYO97_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1753990.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1718..2629
                     /gene="pfkB"
                     /locus_tag="VYO97_001195"
     CDS             1718..2629
                     /gene="pfkB"
                     /locus_tag="VYO97_001195"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1753991.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2626..4578
                     /locus_tag="VYO97_001196"
     CDS             2626..4578
                     /locus_tag="VYO97_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1753992.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKTVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4742..6967
                     /locus_tag="VYO97_001197"
     CDS             4742..6967
                     /locus_tag="VYO97_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1753993.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPQLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7012..7446)
                     /locus_tag="VYO97_001198"
     CDS             complement(7012..7446)
                     /locus_tag="VYO97_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753994.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            7609..9693
                     /locus_tag="VYO97_001199"
     CDS             7609..9693
                     /locus_tag="VYO97_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1753995.1"
                     /translation="MFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKSGVGKSSLL
                     NKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLINDLTVFENLR
                     LALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARALVTGKTLI
                     LADEPTSSLDKENREKVSALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLHFKDHKLVG
                     NCLILNSDEGIKDSKKWSSCLSSSHTKLSVLKHRRPFLPRLLAFFISLVVAIAVTSIN
                     FQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSISTSQNIQT
                     VNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLYQTNTTQRQ
                     IEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEKEGNILKGD
                     GDLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQHPMTKMALD
                     GFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNKGLTYIRQF
                     LMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLKELIGYGKI
                     ISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISGLLPIYTTC
                     RQTVVDTIRKNG"
     gene            9703..10080
                     /locus_tag="VYO97_001200"
     CDS             9703..10080
                     /locus_tag="VYO97_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1753996.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            10240..11382
                     /locus_tag="VYO97_001201"
     CDS             10240..11382
                     /locus_tag="VYO97_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1753997.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDFENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            11391..12605
                     /gene="thiI"
                     /locus_tag="VYO97_001202"
     CDS             11391..12605
                     /gene="thiI"
                     /locus_tag="VYO97_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1753998.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            12740..13553
                     /locus_tag="VYO97_001203"
                     /pseudo
     CDS             12740..13553
                     /locus_tag="VYO97_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            13575..13910
                     /locus_tag="VYO97_001204"
     CDS             13575..13910
                     /locus_tag="VYO97_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV1753999.1"
                     /translation="MHIEHLSHWSGHPNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGRVQFFTLSSVDSESWLATWKNAHDQAEMHRAYERYVIEEAILLS
                     STRQVSLMA"
     gene            13907..14314
                     /locus_tag="VYO97_001205"
     CDS             13907..14314
                     /locus_tag="VYO97_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1754000.1"
                     /translation="MMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYY
                     NDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKLKEAFDQK
                     QIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            14327..15493
                     /locus_tag="VYO97_001206"
     CDS             14327..15493
                     /locus_tag="VYO97_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1754001.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            16001..17494
                     /locus_tag="VYO97_001207"
     CDS             16001..17494
                     /locus_tag="VYO97_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1754002.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            17507..>18004
                     /locus_tag="VYO97_001208"
     CDS             17507..>18004
                     /locus_tag="VYO97_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1754003.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKF"
BASE COUNT         5466 a         3251 c         3909 g         5378 t
ORIGIN      
        1 agaccgaata atataaggat tgattttata gtttttaaac tcaaatgaat tgaaataaag
       61 agagtacgaa aattctcatc tgaaagtatt ttagaataat tctcttcgtg aatttcttca
      121 aaacagatag cttcatctta ggtatgtgat ttctttttgc atttttgagt tagataaggt
      181 ataatggttt tattgtcttt tggggtcgtt acggattcga caggcattat gaggcatatt
      241 ttgcgactcg tgtggcgacg taaacgctca gttaaatata actgcaaaaa ataacacttc
      301 ttacgctcta gctgcctaaa aaccagcagg cgtgacccga tttggattgc tcgtgttcaa
      361 tgacaggtct tattattagc gagatacgat taagccttgt ctagcggctt gataagagat
      421 tgatagactc gcagtttcta gacttgagtt atgtgtcgag gggctgttaa aataatacat
      481 aacctatggt tgtagacaaa tatgttggca ggtgtttgga cgtgggttcg actcccaccg
      541 gctccattat tcctttgcat tcttttgcat tccttggtaa aacgttgtta aatcaacgtt
      601 ttttattttt atctttggta ttcctttgca ttcttttgct aaaaagggag tcacaaacag
      661 accctatttt aaaaaaagga tagaaaaaag gatacaacat ttgtcgcatc ctaaaaataa
      721 tcttttttcg acggaagaca tgggattcga acccacgcac gctattacac gcctaccgcg
      781 tttccaacac ggcctcttaa gcctcttgag taatcttcca atacttactc aaatagtcta
      841 ccataaaggc tcttatcttg caataaaaat tctagaaata agaaaaatga tagattttga
      901 aagaaaatga taaaaaatgc ttgacttcga aagaaagtat gatagaatga atagtgtaaa
      961 cgataacagg aggtgattca gtgttaaaaa cagaacgtaa acaactaatt ttagaggagt
     1021 taaatcagca tcatgtagtt tctctagaaa aattagttag tttgctagaa acgtcagaat
     1081 caacggttcg aagagacttg gatgagttgg aagcggaaaa caagcttcgt cgtgtgcatg
     1141 gtggagcaga actcccccac tccttacagg aagaagaaac cattcaagaa aaatctgtca
     1201 aaaaccttca agaaaagaag ttgctggctc agaaagcagc ctctctcatt aaagaaaaag
     1261 atgtcatctt tatcgatgct ggaacaacaa ctgctttttt gattcatgaa ttggtcaata
     1321 agaatgttac agttgtgacc aactccattc accatgccgc tcagttggtt gaaaagcaga
     1381 ttccaactgt catggttgga ggaaacgtca agacggcgac agatgctagt atcgggggcg
     1441 ttgctcttaa ccagattaac caattgcact ttgaccgtgc ctttatcgga atgaatggtg
     1501 ttgacgatgg ctattatacg actcctgata tggaggaggg agctgtgaaa agagctattt
     1561 tggagaatgc caagcagacc tacgtcttgg tggattcgtc aaaaattgga caaacttgct
     1621 ttgccaaggt agcaccactc aaacgcgcta tcgttatcac tagtcaaggg catgagctct
     1681 tgcaggttat taaggagaaa acggaggtaa tagaagtatg atttatacag tcacactcaa
     1741 tccatccatt gactatatcg ttcgtttgga ccaagtcaaa gttggtagtg taaatcgtat
     1801 ggacagtgat gataagtttg ctggtgggaa aggaatcaat gtcagccgtg tcttgaaacg
     1861 tttgaatata ccaaatacag cgacgggatt tatcggtggc tttactggta aatttatcac
     1921 agatacttta gcagaggaag aaatcgagac acgttttgtc caggtggcag aagatactcg
     1981 tatcaatgtt aaaatcaaag cagaccaaga aacagaaatc aacggaacgg gtccaactgt
     2041 tgaatcggtt cagctagaag aattgaaagc tattttatct agtctgacag cagaagatac
     2101 agttgtcttt gcaggttcaa gtgctaaaaa tctaggcaat gttatctata aggatttgat
     2161 ttccttgacg cgccagactg gtgcgcaagt ggtctgtgac tttgaaggac agaccttaat
     2221 tgatagtttg gactaccagc ctcttcttgt aaaaccaaac aatcatgaac ttggagcgat
     2281 ttttggggtt aaactcgaaa gtttagatga aattgagaaa tacgctcgtg agttactggc
     2341 taagggtgct caaaatgtta ttatctctat ggctggtgat ggtgcccttc ttgtcacatc
     2401 tgagggagct tacttcgcta aaccaatcaa aggaacagtc aaaaattcag ttggagctgg
     2461 tgattctatg gttgctggat tcacaggtga atttgtcaaa tcaaaagacg tagtagaagc
     2521 cttcaaatgg ggagtggctt gcggaacggc aactaccttc tcagatgact tggcaacggc
     2581 ggaatttatt aaagaaacat atggaaaagt tgaggtagaa aaaagatgaa aattcaagac
     2641 ctattgagaa aagatgtcat gttgctagat ttgcaggcaa ctgaaaaaac agctgtcatc
     2701 gacgagatga ttaaaaattt gacagagcac ggttatgtaa cagattttga aacatttaaa
     2761 gaaggaattt tggcgcgtga agctttgact tctactggtt tgggtgatgg aatcgcaatg
     2821 cctcacagca aaaacgctgc tgtcaaagaa gcgacagttc tatttgctaa gtcaaataag
     2881 ggtgttgact acgagagctt ggatggacaa gcaactgacc tcttcttcat gattgcagct
     2941 ccagaaggtg ccaatgatac tcacttggca gccttggcag aattgtctca atacttgatg
     3001 aaagacggtt ttgcagacaa acttcgtcaa gcaacatctg cagaccaagt tatcgaactt
     3061 tttgaccaag cttcagaaaa aactgaggaa cttgttcaag cacctgctaa tgactctggt
     3121 gactttatcg tagctgttac agcttgtaca acaggtattg cccacactta catggcccaa
     3181 gaagcccttc aaaaagtagc tgctgaaatg ggggttggta tcaaggtcga aaccaacggt
     3241 gctagcggtg ttggaaatca actaactgca gaagatatcc gtaaggctaa agctattatc
     3301 attgcagcag acaagaccgt tgaaatggat cgatttgatg gaaaaccatt gatcaatcgt
     3361 ccagttgctg acggtatccg taagacagaa gagctaatta acttggctct ttcaggagat
     3421 actgaagtct accgtgccgc taatggtgcc aaagctgcaa cagcctctaa cgaaaaacaa
     3481 agccttggtg gtgccttgta caaacacttg atgagtggtg tatctcaaat gttaccattc
     3541 gttatcggtg gtggtatcat gattgccctt gccttcttga ttgacggtgc tttgggtgtt
     3601 ccaaatgaaa accttggcaa tcttggttct taccatgagt tagcttctat gttcatgaaa
     3661 attggtggag ctgcctttgg tttgatgctt ccagtctttg cgggttatgt tgcctactct
     3721 attgctgaaa aaccgggttt ggtagcaggt ttcgtggctg gtgctattgc caaagaaggt
     3781 tttgcctttg gtaaaattcc ttatgccgca ggtggtgaag caacttcaac tcttgcaggt
     3841 gtctcatctg gtttcctagg tgcccttgtt ggtggattta tcgcaggtgc cttggttctt
     3901 gccatcaaga aatacgttaa agttcctcgt tcactcgaag gtgctaaatc aatccttcta
     3961 ttgccacttc ttggaacaat cttgacagga tttgttatgc tagctgtgaa tatcccaatg
     4021 gctgcaatca acactgctat gaatgacttc ctaggcggtc ttggaggagg ttcagctgtc
     4081 cttcttggta tcgtccttgg tggaatgatg gctgttgaca tgggtggacc agttaataaa
     4141 gcagcttatg tctttggtac aggtacgctt gcagcaactg tttcttcagg tggttctgta
     4201 gccatggcag cagttatggc tggaggaatg gtgccaccac ttgcaatctt tgtcgcaact
     4261 cttcttttca aagataaatt tactaaggaa gaacgtaact ctggtttgac aaacatcatc
     4321 atgggcttgt catttatcac tgagggagcg attccatttg gtgccgctga cccagctcgt
     4381 gcgattccaa gcttcatcct tggttcagca gtagcaggtg gactcgttgg tcttactggt
     4441 atcaaactca tggcgccaca cggaggaatc ttcgttatcg cccttacttc aaatgctctc
     4501 ctttacctcg tttctgtctt ggtaggagca atcgtaagtg gtgtggttta tggttaccta
     4561 cgcaaaccac aagcataaaa aatagaaaaa tgaaaagatt ggaccgtttg gtgcagtctt
     4621 tttctcttcc cgaaatgcct gtgaaatatg gtataataga agaatggcaa acaagaatac
     4681 aagtacaaca agacggagac cgtctaaagc agaactggaa agaaaagaag cgattcaacg
     4741 aatgttgatt tcattaggaa ttgcgatttt attgattttc gcagccttca aattaggggc
     4801 tgcaggtata accctttata atttaattcg cttgctagtg ggtagcctag cttatctggc
     4861 gatattcggc ctattaatct atctcttctt tttcaagtgg atacgaaaac aggaaggact
     4921 cttatctggc tttttcacca tatttgctgg cttactcttg atttttgaag cctacttggt
     4981 ttggaaatat ggtttggaca agtccgttct aaaagggacc atggctcagg ttgtgacaga
     5041 tctgactggt tttcgaacga ctagctttgc tggagggggc ttgatagggg tcgctcttta
     5101 tattccaaca gcctttctct tttcaaatat cggaacttac tttattggtt ctatcttgat
     5161 tttagtgggt tctctcctag tcagcccttg gtctgtttac gatattgctg aatttttcag
     5221 tagaggcttt gccaaatggt gggaagggca cgagcgtcga aaagaggaac gctttgtcaa
     5281 acaagaagaa aaagctcgcc aaaaggctga gaaagaggct agattagaac aagaagagac
     5341 tgaaaaagcc ttactcgatt tgcctcctgt tgatatggaa acgggtgaaa ttctgacaga
     5401 ggaagctgtt caaaatcttc cacctattcc agaagaaaag tgggtggaac cagaaatcat
     5461 cctgcctcaa gctgaactta aattccctga acaggaagat gactcagatg accaagatgt
     5521 tcaggtcgat ttttcagcca aagaagccct tgaatacaaa cttccaagct tacaactctt
     5581 tgcaccagat aaaccaaaag atcagtctaa agagaagaaa attgtcagag aaaatatcaa
     5641 aatcttagaa gcaacctttg ctagctttgg tattaaggta acagttgaac gggccgaaat
     5701 tgggccatca gtgaccaagt atgaagtcaa gccggctgtt ggtgtaaggg tcaaccgcat
     5761 ttccaatcta tcagatgacc tcgctctagc cttggctgcc aaagatgtcc ggattgaagc
     5821 accaatccct gggaaatccc taatcggaat tgaagtgccc aactccgata ttgccactgt
     5881 atctttccga gaactatggg aacaatcgca aacgaaagca gaaaatttct tggaaattcc
     5941 tttagggaag gctgttaatg gaaccgcaag agcttttgac ctttctaaaa tgccccactt
     6001 gctagttgca ggttcaacgg gttcagggaa gtcagtagca gttaacggca ttattgctag
     6061 cattctcatg aaggcgagac cagatcaagt taaatttatg atggtcgatc ccaagatggt
     6121 tgagttatct gtttacaatg atattcccca actcttgatt ccagtcgtga ccaatccacg
     6181 taaagccagc aaggctctgc aaaaggttgt ggatgaaatg gaaaaccgtt atgaactctt
     6241 tgccaaggtg ggagttcgga atattgcagg ttttaatgcc aaggtagaag agttcaattc
     6301 ccagtctgag tacaagcaaa ttccgctacc attcattgtc gtgattgtgg atgagttggc
     6361 tgacctcatg atggtggcca gcaaggaagt ggaagatgct atcatccgtc ttgggcagaa
     6421 ggcgcgtgct gcaggtatcc acatgattct tgcaactcag cgtccatctg ttgatgtcat
     6481 ctctggtttg attaaggcca atgttccatc tcgtgtagca tttgcggttt catcaggaac
     6541 agactcccgt acgattttgg atgaaaatgg agcagaaaaa cttcttggtc gaggagacat
     6601 gctctttaaa ccgattgatg aaaatcatcc agttcgtctc caaggctcct ttatctcgga
     6661 tgacgatgtt gagcgcattg tgaacttcat caaggctcag gcagatgcag actacgatga
     6721 gagttttgat ccaggtgagg tttctgaaaa tgaaggagaa ttttcggatg gagatgctgg
     6781 tggtgatccg ctttttgaag aagctaagtc tttggttatc gaaacacaga aagccagtgc
     6841 atccatgatt cagcgtcgtt tgtcagttgg atttaaccgt gcgacccgtc tcatggaaga
     6901 gcttgagatg gcaggtgtca ttggtccagc tgaaggtacc aaaccaagaa aagttttaca
     6961 acaataaaaa aatagcttct ttccaagttt ggaaggaggc tattttagtg attattgatt
     7021 agttttattt tccgaagttg gggtattgga ctgtttttcg ttttcagtag caggtttact
     7081 tggagtagga gcaggagtag aagaatcctg agttgatgtt ttctgctctt cttttttctc
     7141 ttccttgacg ctggattttg gcgtttcttc ttgttgtgtt ttttcttgag aagtcttatt
     7201 ttccttattg ggactagtat tttcctgagg ggattccttt tgaatttctt tgacaattgt
     7261 tgtcgtctgg cttgttgtag gttccttttt agtatttttg ttattatcca aggcgttcat
     7321 aatcgtgata ctagcggtaa tcaagccaag gatactagcg atagtcgcaa cggttttttg
     7381 aaagactgta aagccttttt tgatgtgttc tgtttttggt tttgaagttc tacgataatt
     7441 agacatacat tctctccttt aattaaaatt tattatacct catttcttta aaaaaatctt
     7501 aaaaaaagag gagatttttc tacacgaact tcagtaatta gttgaaataa aatatgactt
     7561 atgatataac aaaataaaga aagcgatttc aaaaaaggag ggcgttatat gttcgaaatt
     7621 agaaatttga gtctctcttt tgcaggcagg cggttgctag agccaacaga tttgatattt
     7681 gaaaggggaa aagtatatac aatatatggg aagtcaggcg taggaaagtc ttctttactg
     7741 aataaaattg gtttgctctc tgctacggat cctagtgttt cttatttctt cgatggaaaa
     7801 gagattgata tcgaaaataa gaaatcagtc tctgtcttta tagctgaaga agttgccttt
     7861 gtatttcagg ggcgaaatct aataaatgat ttgaccgtat ttgaaaattt aagactagct
     7921 ttaaactttt atgacttgag tccagatgaa atagaaagca aaattgatac agtattggcg
     7981 gatttacaga tttcttcttt aaagcatgct tatccagaag atttatccgg tggtgaagaa
     8041 caacgagtgg caatcgcaag agccttagtt acaggaaaga ctctcatctt agctgatgaa
     8101 ccaacaagtt ctttggataa agaaaataga gaaaaagtct ccgccttgtt aattgattta
     8161 gctcacaaat atcataaaat tgtattgatt gtttcgcatg atgaggcgat gattgcaata
     8221 ggagatgttc agcttcattt taaagaccat aaattagttg ggaattgtct tattttgaat
     8281 tctgatgaag ggataaagga ttctaagaag tggtcctcct gtttgtcgtc ttctcatacg
     8341 aagctatccg ttttgaaaca tagaaggcct tttttaccta gattattagc gttttttatc
     8401 tctcttgtag ttgctatagc agtaacctct attaactttc agtccttatt tgcagataag
     8461 tatcaccaac tggtgaacaa ctcactcgaa aatggcttct tggtgattaa tgattcgctt
     8521 catttacaaa ctacaaaagt gttggatgat tttctcagtt ttgataaaga ggaaatcacc
     8581 tcaattagca caagtcagaa tattcaaacc gtcaatcctt atatagagtt tctctctttg
     8641 ggtatgacgt ttgataattc aaaagactat tcacaacttc aaaagaactt tcagccaact
     8701 ttgactattg atggtcagac cagaagcttg acaagaaatt tttcaattca acccttgtat
     8761 cagacaaata caacacagag gcagatagag tattttgata ggagcaatga aaaagggatt
     8821 tatctatctg agcaattcat ttcagatgaa aaattaccaa atatagtttc gggaactacc
     8881 gtgacactga ctttttatat tccaatttct ttatatgaat caagtataga gaaagagggg
     8941 aatatattga aaggagatgg cgatttattt gtcaaagtgg ataaaacata tcctgtttta
     9001 gggattgtta aaaaaagtta tccgtttgaa tattcccctt atgggaacac cctatttatg
     9061 aatattgctg aaatggaaga attgcaaaat gaagcaattc aacagcatcc catgacgaag
     9121 atggctttag atggttttcc tttgaaatca tggatgccga gtgcgattca tgtgttacta
     9181 aaggatagcg gtgctattcc tgaagaattg agtagaataa gagcaatctc ttcacaaatt
     9241 actattttat catcatatga aaactacgaa gaatttaata aaggactcac ctatatcagg
     9301 caatttttaa tgttgctatc cctagtctta ctcatccttg tcatcgcagt attgtccttt
     9361 gttttctttt tgctcaatcg tcctcgacgc tttgaagtag ggattttaaa atctttggga
     9421 tacagtactc aaaatattgt ttggcttttc ttaaaagaat taattggtta tggtaaaata
     9481 atttcgatat tagcaagttg tttactcgtc attctttcca ttttagcaat gcaagtgctt
     9541 aaattggaaa tttcagatgt ttttaaattc tatctaactt ccgttttcac cttaattgga
     9601 ttatcagtct cagtactgat aataagtggc ttactaccta tatacacaac ttgtaggcaa
     9661 acagttgtcg atactatccg aaagaatgga tgaggtgata tcatgaaaca taagataatg
     9721 aaaaaaataa tagtggtagt gtttgctatc ccatggttaa catttttaag tcaagtacat
     9781 gcagattatg taagtaagtc tagttcttta gacggttata gcttggtagg cttgaatgtt
     9841 cttaatgtga cttttaatgc ttctggctat tctgatactt atagtggcag tgtgtcaaat
     9901 atgtatttca ctgactgggc ttggtttcct aatacgataa gtaatcgttc cacttggaaa
     9961 agttcgattc catatggtca acgcgcaaat ggtacagtcc atataggtgt aggagtaaat
    10021 tcgccttggg gagcggttaa tctctggggc ggacaaaatt acttttcttt agatttttaa
    10081 cagaaataat caaagttaga tagaatgaat aagaatgctc ttgatgatgg tatcaagagt
    10141 attttatgtt attcagatta atgcaaacat tgccctttct tgtcgcaggc tccgtttcat
    10201 gatacaatag aaggagtgat tttagaaagc gtgagaaaca tgatttactt tgataattcg
    10261 gcgacgacca ggccctatcc agaagccctt gaaacctata tgcaggtggc ttcaaaaatt
    10321 ttaggaaatc catctagtct ccatcgtttg ggagaccagg caacacgaat tttagatgct
    10381 tcccgtcaac agattgcaga tttaatcggt aagaaaagtg atgaaatctt ctttacatct
    10441 ggtgggacag aaggggataa ctggatcatc aagggagtag catttgaaaa agcccagttt
    10501 ggcaagcaca tcatcgtatc agccattgaa catccagcag ttaaggaatc agccctctgg
    10561 ttgaaagctc aaggttttga agtggatttt gctccagttg ataacaaagg atttgtggat
    10621 gttgaggcgc tagaagattt gataagacct gatacgaccc tcgtttccat catggcagtt
    10681 aacaatgaaa ttggctctgt tcagcccatt gaggctattt caaaactatt ggcagacaag
    10741 ccaactattt ccttccacgt tgatgcggtt caggcgcttg ccaagattcc gactgaaaag
    10801 tatctgacag aacgagtgga tttcgcgact ttctctggtc acaagttcca cggaatccgt
    10861 ggtgttggtt ttatctatat caagtctggc aagaagatta cgcctctttt aacaggtggt
    10921 ggtcaggaac gtgattaccg ttcgacaact gaaaatgtgg caggaattgc agcgaccgct
    10981 aaggctctcc gtttatctat ggaaaagcta gatatcttta ggagcaagac tgggcagatg
    11041 aaggcagtga ttcgtcaagc acttctggac tatccggata tatttgtctt ttcagatgag
    11101 gaagacttcg cccctcatat tctgactttt ggaatcaagg gtgttcgtgg tgaggtcatt
    11161 gttcacgcct ttgaagacta tgatattttc atctcaacga cctcggcttg ttcgtccaag
    11221 gctgggaaac cagccggaac cctgattgct atgggagtgg acaaggataa ggctcagtca
    11281 gctgtgcgtc ttagtctaga ctttgaaaat gatatgagtc aggtcgagca gttcttgacc
    11341 aagctaaaat taatttacaa tcaaactaga aaagtaagat aggagcattc atgcagtatt
    11401 cagaaattat gattcgctac ggtgagctgt caaccaaggg caaaaatcgt atgcgtttca
    11461 tcaataaact tcgcaataat atttcagacg ttttgtctat ctatccccaa gttaaggtaa
    11521 cagcagatcg cgaccgtgcc cacgcttacc tcaatggagc agattacaca gcagtagcag
    11581 aatcgctcaa acaagtattt ggaattcaaa acttttcccc tgtttataag gttgaaaaat
    11641 ctgtagaagt tctgaagtct gctgtccaag agattatgca ggacatctac aaggaaggca
    11701 tgacctttaa aatctctagc aagcgtagcg accacacctt tgaattggac agtcgtgaac
    11761 tcaaccaaac gcttggaggg gctgtattcg aagccattcc aaacgtacaa gctcaaatga
    11821 aaagtcctga catcaatctt caggtggaga tacgtgaaga agcagcctat ctttcttatg
    11881 aaaccgttcg aggggcaggt ggattaccag tgggaacttc tggtaagggc atgctcatgt
    11941 tgtcaggggg aatcgattcc cctgtagctg gctatctagc gcttaaacga ggggtagata
    12001 tagaggcagt tcactttgcc agcccacctt acacgagtcc tggtgctctt aaaaaagccc
    12061 aagatttgac ccgtaaatta accaaatttg ggggaaatat ccagtttatc gaagttcctt
    12121 ttacagagat tcaagaggaa atcaaagcca aagcgccaga agcctacctt atgaccttga
    12181 cgcgtcgttt tatgatgcgt attactgacc gtattcgtga ggtacgaaat ggtttagtta
    12241 tcatcaatgg ggaaagtcta ggtcaagtag ccagccaaac ccttgaaagt atgaaggcta
    12301 tcaatgctgt taccaacact cccatcattc gtcctgtggt taccatggac aagttggaaa
    12361 tcattgacat cgcccaggaa atcgatacct ttgacatttc aatccaaccg tttgaagact
    12421 gttgtaccat ttttgcacca gatcgtccaa aaacaaatcc taaaattaag aatgcggagc
    12481 agtacgaagc gcgtatggat gttgaaggct tggttgagcg agcagtggct ggaatcatga
    12541 ttactgaaat cacacctcaa gccgaaaaag atgaagttga tgacttgatt gacaatctgc
    12601 tctaattcag aaaatccaaa agaatagcga aaatcagtaa aaaaagttag ttttttctct
    12661 aaaaacaggt aaaaaactaa ctttttttat ttttatgata taatgatata aaattttgaa
    12721 tatagagagt tttctgacaa tgaatcaatc ctacttttat ctaaaaatga aagaacacaa
    12781 actcaaggtt ccttatacag gtaaggagcg ccgtgtacgt attcttcttc ctaaagatta
    12841 tgagaaagat acagaccgtt cctatcctgt tgtatacttt catgacgggc aaaatgtttt
    12901 taatagcaaa gagtctttca ttggacattc atggaagatt atcccagcta tcaaacgaaa
    12961 tccggatatc agtcgcatga ttgtcgttgc tattgacaat gatggtatgg ggcggatgaa
    13021 tgagtatgcg gcttggaagt tccaagaatc tcctatccca gggcagcagt ttggtggtaa
    13081 gggtgtggag tatgctgagt ttgtcatgga ggtggtcaag cattttatcg atgagaccta
    13141 tcgtacaaaa gcagactgcc agcatacggc tatgattggt tcctcactag gaggcaatat
    13201 tacccagttt atcggtttgg aataccaaga caaaattggt tgcttgggcg ttttttcatc
    13261 tgcaaactgg ctccaccaac cgctatatcg agtgccagaa actatcgcct gaccaacgca
    13321 tcttcatcta tgtaggaaca gaagaagcag atgatacaga caagaccttg atggatggca
    13381 atatcaaaca agcctatatc gactcgtcgc tttgctatta ccatgatttg atagcagggg
    13441 gagtacatct ggataatctt gtgctaaaag ttcagtctgg tgccatccat agtgaaatcc
    13501 cttggtcaga aaatctacca gattgtctga gattttttgc agaaaaatgg taagttaaga
    13561 aaggaaaaaa cgaaatgcat attgaacatc ttagccactg gagtggtcat cctaaccgtg
    13621 aaatgtacct taaccgttat ggacatggtg ggattccagt tgtggtcttt gcttcatcag
    13681 gtggtagtca caacgaatac tatgattttg gcatgattga tgcctgtgct tcctttatcg
    13741 aggaaggccg tgtccagttc tttaccctat ctagtgtgga tagtgagagc tggttggcta
    13801 cttggaaaaa tgctcatgac caagcggaaa tgcaccgtgc ctacgaacgt tatgtgattg
    13861 aggaggccat tcttttatca agcacaagac aggttagttt gatggcatga tgacgacagg
    13921 ttgctctatg ggagcctatc atgcactcaa tttcttcctc cagcatccag atgtctttac
    13981 caaagtgatt gctctcagtg gtgtttacga cgcacgtttc tttgtcggtg attactacaa
    14041 cgatgatgct atttaccaaa actcgccagt agattatatt tggaaccaaa acgatggctg
    14101 gtttattgac cgttaccgtc aggcagagat tgtgctgtgt acggggcttg gagcctggga
    14161 acaagatggt ttaccatcct tttacaagct caaagaagcc tttgaccaga aacaaattcc
    14221 agcctggttt gctgaatggg gacatgatgt cgcccacgac tgggaatggt ggcgtaaaca
    14281 aatgccttat ttcctcggta atctctattt ataaaaggag ttacctatga attaccttgt
    14341 tatttctccc tactatccac aaaactttca acagtttacc atcgaactag ctaataaagg
    14401 catcacagtc ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc ccttgcgcaa
    14461 tagcttgacc gagtattttc gtgttgataa tcttgagaac atagatgaag tcaaacgtgc
    14521 agttgctttt ctcttttata aacatggtcc aattgaccgc atcgagtctc acaatgaata
    14581 ctggcttgag ctagacgcaa cactcagaga acaattcaat gtttttggtg ccaaaccaga
    14641 ggatctcaaa aagacgaaat ataagtctga aatgaagaaa cttttcaaaa aagcaggtgt
    14701 tcctgtggta cctggagctg ttatcaagac ggaagcagat gttgatcaag cagtgaaaga
    14761 aatcggtctt ccaatgattg ccaaacctga taatggagtg ggagcagccg caacctttaa
    14821 acttgagaca gaagacgata tcaatcactt caagcaagaa tgggaccatt caacccttta
    14881 tttctttgaa aaatttgtca cttccagcga aatctgtacc tttgacgggc tcgtggacaa
    14941 ggatggaaag attgtcttct caacaacctt tgactacgcc tatacaccgc ttgacctcat
    15001 gatttataag atggacaatt cttattatgt gctcaaggat atggatccta aactgcgcaa
    15061 gtatggggaa gcaattgtca aagaatttgg tatgaaagaa cggtttttcc atattgagtt
    15121 cttccgtgag ggggacgatt atattaccat cgagtacaat aaccgccctg caggtggttt
    15181 taccattgat gtttataact ttgctcattc cttggacctt tatcgtggct atgcagctat
    15241 tgtcgcagga gaggagttcc cggcgtcaga ctttgaaact cagtattgtt tggctacttc
    15301 tcgccgtgca aatgctcact atgtttattc agaagaggat ttgcttgcca aatatagcca
    15361 gcagttcaag gttaaaaaag tcatgccagc tgccttcgcg gaacttcaag gagattacct
    15421 gtatatgctg acaactccga gtcgacaaga aatggatcag atgattgctg atttcggaca
    15481 acgtcaagaa taagaactat cggattaagg aaattcactc ccttaatcct tttgttttgt
    15541 ctggtaaaaa ataagagcat cctaacaagg tagctatcat aaaacttgtt cgataactat
    15601 ttgaagcagg attaggtggc tagaaattga attttaacat ttcaatttag tcatagtatt
    15661 gtgttttcgt atccttaaat cagctaaaag gatccatgac gacacctata cgatatagtt
    15721 ttcaggatac caaaccagtc tattaatact caatgaaaat caaagagcaa actaggaagc
    15781 tagccgcagg ttgctcaaaa cactgttttg aggttgtgga tagaactgac agagtcagta
    15841 tcatatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtataaa
    15901 atattcaggt gacgcataga tatagttaat tgaagctttg tttgaaatct gataaaataa
    15961 tgatattact aagttttaaa aactaaagaa aagggaagat atgattacag gcgaattaaa
    16021 aaataaaatc gatcagctgt gggaaattct ttggacagaa ggaaacgcaa atcctttaac
    16081 aaatattgaa cagttgactt atctcttatt tatgaaagat ttggatagtg tcgagcttgg
    16141 acgtgaaagt gatgctgaat ttctagggat tccttatgag ggagtttttc caaaagataa
    16201 acctgaatac cgttggtcaa cttttaaaaa tataggagat gctcaggaag tttatcgttt
    16261 aatgactcag gagatttttc cgtttattaa aaatctcaag ggggatacag atgatacagc
    16321 cttttcacga tatatgcgag aagctatttt tcaaataaat aaacctgcta cgcttcaaaa
    16381 ggcaatttct atcttagatg tttttccaac taggggatta gatgtagatt ttgataatga
    16441 caaacaaagt attactgata tcggagatat ctatgaatat ctgttatcaa aattgtcgac
    16501 cgcaggtaaa aatggacagt tccgtacacc tcgtcacatc atcgatatga tggttgagtt
    16561 gatgcaaccg actatcaaag atatcatctc agatcccgct atgggttctg ctggcttctt
    16621 agtatctgct agccgttact taaagcgtaa gaaagatgaa tgggaaacca atacagataa
    16681 tatcaatcat tttcataatc agatgtttca tggaaatgat acggatacga ctatgttgag
    16741 acttggggcg atgaacatga tgctacatgg agtagaaaat ccacaaatca gttaccttga
    16801 ctcgctgtct caagataatg aagaagccga taaatatact ttggttttag caaatcctcc
    16861 ttttaagggc tcacttgact acaattcaac ctctaatgac cttcttgcaa ccgtaaaaac
    16921 caaaaaaaca gaattactct ttctttctct tttcttgcga actttaaaac caggtggacg
    16981 agcagcagtt atcgtacctg atggtgtcct ttttggttcg tctaaagctc ataaaggaat
    17041 tcgtcaggaa attgtagaga atcataagct tgatgctgta atctcaatgc ctagtggtgt
    17101 gttcaagcct tatgctggag tttcaactgc cattctcatc tttacaaaaa ctggtaatgg
    17161 tggtactgac aaagtctggt tttacgatat gaaagcggat ggtttaagtt tggatgataa
    17221 gcgacaaccg attagcgaca atgatattcc agatattatc gaacgctttc atcatcttga
    17281 aaaagaagca gaacgtcaga gaacggatca atctttcttt gttccagttg ctgagataaa
    17341 ggaaaatgat tatgatttgt ctatcaataa atataaagag attgagtatg aaaaagttga
    17401 gtatgaacca acagaagtca tattaaagaa aatcaatgat ttagaaaaag aaattcaagc
    17461 tggcttggct gaattggaaa aattactcaa gtagggaggt ggctgtatga aaaaagtgaa
    17521 gttgggggaa gtttgtgaga ttttatctgg ttacgctttt aaatcatcac aatttaatga
    17581 taacaaaatt ggtctcccct taatacgaat tcgagatgta gaaagaggtt tttctgatac
    17641 ctactttgaa ggaacttatc ccgaagaata tcttattaaa aatggagact tattgattac
    17701 aatggatggc agttttatat tgaaaaaatg ggagggggat ttagcgctac ttaatcaacg
    17761 tgtctgtaaa attaaaatta cggataaaag tgtagatgaa ggttatatat cttggttgat
    17821 tcctaaattt ttaaaagaaa ttgaagataa gacccctttt gttacagtga aacatctttc
    17881 agtagctaaa ataaaagata tctcatttgt attgcctaat aaactagaac aaaagctaat
    17941 tgcaaaaaaa ctaaatacga taagtcaaat ttatgatttt cgtaaaattc aatcagaaaa
    18001 attt
//
LOCUS       DAVMAF010000034        17881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMAF010000034 DAVMAF010000000
VERSION     DAVMAF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.034"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            668..1472
                     /locus_tag="VYO97_001209"
                     /pseudo
     CDS             668..1472
                     /locus_tag="VYO97_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1625..1879
                     /gene="blpM"
                     /locus_tag="VYO97_001210"
     CDS             1625..1879
                     /gene="blpM"
                     /locus_tag="VYO97_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1754004.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATSGF"
     gene            1895..2098
                     /gene="blpN"
                     /locus_tag="VYO97_001211"
     CDS             1895..2098
                     /gene="blpN"
                     /locus_tag="VYO97_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1754005.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            2342..2575
                     /locus_tag="VYO97_001212"
     CDS             2342..2575
                     /locus_tag="VYO97_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /protein_id="HEV1754006.1"
                     /translation="MDTKMMSQFSVMDNEMLVRTEDGDVSDIYRGYANQRSPFASYPS
                     ILKNSGPFPVSGYCLRGYHDRGYIGAGFHLCGI"
     gene            2607..3296
                     /locus_tag="VYO97_001213"
     CDS             2607..3296
                     /locus_tag="VYO97_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1754007.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVVVLHISFNVQTAKH
                     VQSTAEGWNVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3338..3571
                     /locus_tag="VYO97_001214"
     CDS             3338..3571
                     /locus_tag="VYO97_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754008.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3722..4333
                     /locus_tag="VYO97_001215"
     CDS             3722..4333
                     /locus_tag="VYO97_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1754009.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4494..5288
                     /locus_tag="VYO97_001216"
     CDS             4494..5288
                     /locus_tag="VYO97_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1754010.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5285..5920
                     /gene="trmB"
                     /locus_tag="VYO97_001217"
     CDS             5285..5920
                     /gene="trmB"
                     /locus_tag="VYO97_001217"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1754011.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6046..6525
                     /gene="rimP"
                     /locus_tag="VYO97_001218"
     CDS             6046..6525
                     /gene="rimP"
                     /locus_tag="VYO97_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1754012.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTHKKTVRIPYSLVSKARLAVKL"
     gene            6569..7705
                     /gene="nusA"
                     /locus_tag="VYO97_001219"
     CDS             6569..7705
                     /gene="nusA"
                     /locus_tag="VYO97_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1754013.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VTIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7727..8020
                     /locus_tag="VYO97_001220"
     CDS             7727..8020
                     /locus_tag="VYO97_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1754014.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8013..8312
                     /locus_tag="VYO97_001221"
     CDS             8013..8312
                     /locus_tag="VYO97_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1754015.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8329..11121
                     /gene="infB"
                     /locus_tag="VYO97_001222"
     CDS             8329..11121
                     /gene="infB"
                     /locus_tag="VYO97_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1754016.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11372..11722
                     /gene="rbfA"
                     /locus_tag="VYO97_001223"
     CDS             11372..11722
                     /gene="rbfA"
                     /locus_tag="VYO97_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1754017.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12001..12780
                     /locus_tag="VYO97_001224"
     CDS             12001..12780
                     /locus_tag="VYO97_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754018.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDSSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWVSREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            12782..12964
                     /locus_tag="VYO97_001225"
     CDS             12782..12964
                     /locus_tag="VYO97_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754019.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(13304..13381)
                     /locus_tag="VYO97_001226"
                     /pseudo
     CDS             complement(13304..13381)
                     /locus_tag="VYO97_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13412..13645
                     /locus_tag="VYO97_001227"
     CDS             13412..13645
                     /locus_tag="VYO97_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1754020.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            13645..14979
                     /locus_tag="VYO97_001228"
     CDS             13645..14979
                     /locus_tag="VYO97_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1754021.1"
                     /translation="MTDERIHVLRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            14989..15243
                     /locus_tag="VYO97_001229"
     CDS             14989..15243
                     /locus_tag="VYO97_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1754022.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            15339..15734
                     /locus_tag="VYO97_001230"
     CDS             15339..15734
                     /locus_tag="VYO97_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1754023.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            16147..16596
                     /locus_tag="VYO97_001231"
     CDS             16147..16596
                     /locus_tag="VYO97_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV1754024.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQISILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPEFLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            16583..17140
                     /locus_tag="VYO97_001232"
     CDS             16583..17140
                     /locus_tag="VYO97_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754025.1"
                     /translation="MARTELSDKIETERLVLRVRTVADAEDIHAYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIAGSVDFNHRYGDDVLELGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEE"
     gene            17142..>17881
                     /locus_tag="VYO97_001233"
     CDS             17142..>17881
                     /locus_tag="VYO97_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1754026.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKL"
BASE COUNT         5529 a         3172 c         4029 g         5151 t
ORIGIN      
        1 aattttatca gtattgtttg aaatccaaaa aaagtatgtt aagctatatt tatttggttc
       61 tttagtttga ttgttgttga gtataactat ttttgtatag atgaaaatct tgttattgtt
      121 caagatgttt tgatgaccat tcatgatttg agtaatccaa ggtcaaaatg agtgctatac
      181 tagcagtgta aagattcttg ttcaactaag ataaatcaca catttaggag gaaattttat
      241 gaagaaaaaa atacttatca ttttcatctt gtatctaatc atgtccatct ttctttatcc
      301 gcttagggaa agtatttggt atcagctatt ttataccata gcctatgtga ttgcagttat
      361 gatctatttt gctttaacta aaaagaaagg agcaaagaaa tgaaaacttt tcttgctaaa
      421 aaacggaaca tcttcctttc gtttgggggt tgaagtatta taaaaagtag aagttaatga
      481 agtataaaag ggtaagtgtt tttgcaatac ttaccctttt ataatagatt ccctgcgaaa
      541 caaaatatgg tatagtatag tagattgaaa ctagaatagt acgcctctac ttctaaaaca
      601 ttgttagaaa tcgatttgac tgtcctgatt gatttatcct gttcttattt cattttacta
      661 tagttctatg aattatgaag gaagtaaaca actaactgat gcacgattta aatgtcttgt
      721 tggtgttcaa cgcacgactt ttgaagagat gttagctgta ttaaaaacag cttatcaact
      781 taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc ttatggccac
      841 tcttcactat gtgcgagaat atcgaactta tgaagaaatt gcggctgatt ttggtatcca
      901 cgaaagcaac ttactccgtc ggagccaatg ggttgaagta actcttgttc aaagtggtgt
      961 tacgatttca agaactcctc tcagctctga ggacacggta atgattattg atgcgacgga
     1021 agtataaatc aatcgcccta aaaaaagaat tagcgaatta ttctggtaaa aagaaatgcc
     1081 acgctatgaa ggctcaagcg attgtcacaa gtcaagggag aattgtttct ttggatatca
     1141 ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag tcgcagaaat atcggacaag
     1201 ctggtaaaat cttgactgac agtgattatc aagggcccat gaagatatat cctcaagcac
     1261 aaattccacg taaatccagc aaactcaagc cactaacagc tgaagataaa gcctataacc
     1321 atgcgctatc taaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta
     1381 aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga
     1441 ttgctggtat tatcaatcat gaactaggat tttggtttcg caggaagtct aatgtaattt
     1501 ttgcaattca agacgtttcg atgactattc aagatctgga tgaaaaattt taaaaaacga
     1561 tgatatacta aacttgtcaa agttacaaca agacaaaaat taaaaataaa aaagagtatt
     1621 tgtcatggat acaaaaatta tggaacaatt tcatgaaatg gatataacta tgttatctag
     1681 tattgaagga ggcaagaata attggcaaac taatgtctta gaaggtggtg gtgctgcttt
     1741 tggtggctgg gggttaggga cagctatttg tgctgcgagt ggtgttggag cgccatttat
     1801 gggagcatgt ggatacatag gagctaaatt tggtgtggct ctttgggcag gagtaacagg
     1861 ggcaacgagt ggattttaac aaaaggagac ttgtatgaat acttattgta atataaatga
     1921 aacaatgctg agtgaggttt atggaggtaa ttccggagga gcagctgtag ttgctgcttt
     1981 aggttgtgca gcgggtggag tgaaatatgg gagacttcta ggaccatggg gcgctgcaat
     2041 aggaggaatt ggaggagcag tggtttgtgg atatttagcc tataccgcta catcataaaa
     2101 atgaaagcta aattttatga aggctatatt ctttattatt ctcttcgctt ttcaaaccta
     2161 tcttatttat ctatccataa gtatctccga taaaaaacaa aagactattg aattaacatc
     2221 attgaattgt tttgtcatat tatttctaat ctatgataaa ttaatttttc tctttattgc
     2281 ttatgttttt ttgataattt ttatattaaa cttgttccgt aactgaaaag tgatatacta
     2341 tatggataca aaaatgatgt cacaattttc tgttatggat aatgaaatgc ttgttcgtac
     2401 agaggatggg gatgtctctg atatttatag agggtacgct aatcagagaa gtccattcgc
     2461 ttcttaccct tctatattaa aaaattcagg tccttttcca gtaagtgggt actgcctgcg
     2521 tggttatcat gaccgtggtt atataggagc aggttttcat ttatgtggaa tataggctga
     2581 aatacaagaa atgagggagg atttctatga aaaagtatca acttctattc aaaataagtg
     2641 cagtcttctc ttacttattt ttcgtatttg gtctttctca gctgacgctt atcgtccaaa
     2701 attattggca attttcttcc cagattggca atttcgtctg gattcaaaat atcttgagtt
     2761 tactatttag cggagtcatg atttggattc tggttaagac aggccatggc tatctctttc
     2821 gcattccaag aaaaaaatgg ctttggtatt ctattttgac agtattagtg gtagtgctcc
     2881 atatctcttt taacgttcag acagctaaac atgttcagtc aactgctgag ggttggaacg
     2941 tattgattgg ttatagtggg accaactttg ctgagctagg tatctatgta accctgtttt
     3001 tcctgactcc actaatggaa gagttgattt ataggggatt acttcaacac gccttcttta
     3061 agcattcgcg atttggcctt gatttgcttc ttccttccat tttgtttgct cttcctcatt
     3121 ttttgagtct gcctagtctg ttagatatct tcgtctttgc aacatttgga atcatctttg
     3181 ccggtttgac ccgctatacc aagagtattt atccatccta tgcggtgcat gtgatcaata
     3241 atattgtagc gaccttcccg tttttgctca cttttctaca tagggtcttg gggtaaaaaa
     3301 acaaaaggct tgcttttcag ccatagagga ggtcatcatg tataaacact tatttttcct
     3361 agattccaaa actttagatc ggttgacacc ctatattcta gtcttggctt ctgacaccat
     3421 tgcttttaat gtttttgtgc taacctttgt atctgcggtg gtttttaatt tcctaaattc
     3481 catgctagct ttaatggcta tattcatagg ggctggctat gtggtcggat tttggttact
     3541 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgt acttgagtac
     3601 ggcaaggcga cgttgacgtg gtttgaattt gattttcgaa gagtataaaa tggtttgttt
     3661 tggaaaggct agaactaaag aatgacgtgt aggaagtctg tttgttgagg aggataattt
     3721 tatggagttt tttgataaat ttcatgcctt gtgttttgga tttttagtac taataattgt
     3781 cattacagtt ccttatacga ttaaccatgg ggattttttt caaaatgaat ctgcattgat
     3841 tattgtaagt cttcttgtaa cctcgctgag tgttgcttat gctagaaagt ttgaaatgat
     3901 ttcttttggg atgttaagca agaaacaact tttgcttttc attgcaatct ttcttctaag
     3961 tgtacttgag acgctggttt atattcattt cttcgctgtt tcttctggct caggggtcca
     4021 acacttggcg gaagtcagca gaggaatttc cctgtctttg attttgacta cctcagtttt
     4081 tggccccatc caggaggaac tcattttcag aggacttctt caaggtgcgg tttttgacaa
     4141 ttcttggtta gggcttgtgc taacttcctc tctcttttct ttcatgcatg gaccttctaa
     4201 tgtcccttcg tttatttttt atctacttgg gggcttgttg ctgggccttg cttataaaaa
     4261 gagccaaaac ctatgggttt ctactctagt ccacatgttt tacaacagtt ggccactcct
     4321 atattattta taaaaattat gaaaaggtaa gcagaagacg gtgcttacct ttttcaagct
     4381 ataatgatac ccaagccaat ccagaggctt ttctttgaga atcgggttag agtggttgac
     4441 gaataggcca aaaactagta gaatagtaag gaaactttat acggaggaaa gaaatggatt
     4501 tgggtgataa tgagctaaca ctgactccca tacctgggaa aagtggcaag gcttatatgg
     4561 gtagctatcc tgatgggaag cgcatctttg taaaaatgaa cacctctcca atcctacctg
     4621 gtctagctag agaacaaatt gctccacaat tattatggag tcgtcgtttg gcagatgggc
     4681 gtgatatgtg tgctcaagaa tggttgacag gcaagatatt gaccccctat gatatgaatc
     4741 gtaagcaaat cgtcaatatt ttaacccgtc ttcatcgctc acgtccgttg atgacacaat
     4801 tgagtcgttt gggctatgcc atggaaacac ctgtagattt actacagtct tggcaagaaa
     4861 cggctccaga tgctttgcgt aaaaatcatt ttatcagtga agtgatggct gatttgcgtc
     4921 agactattcc aggatttaga gaggaccatg cgaccattgt ccatggagat gtacgacata
     4981 gtaattggat tgagacagat agtggcttga tttatttagt agattgggat tcggttcgct
     5041 tgaccgatcg catgtttgat gtggcccata tgctctgcca ttatatttca gaacatcagt
     5101 ggaaggaatg gttgacctac tacggttaca agtacaatca aacggtatta agtaaattgt
     5161 attggtatgg tcaattgtct tatttgagtc agatttccaa gtattatatg aaccaagatt
     5221 tagaaaatgt caatcgggag attcatggtt tgcgtcattt ccgagacaag tatggaaaga
     5281 gaagatgaga gttagaaatc gtaaaggggc aacagaatta ctagaggcaa atccccagta
     5341 tgtggtcctc aatcccttgg aagccaaggc aaaatggcgg gacttgtttg gcaatgataa
     5401 tcccattcat gtggaagttg gaagtggaaa gggtgccttt gtttcaggta tggccaagca
     5461 aaaccctgac atcaactata tcgggattga tattcaaaag tctgttttga gctacgcttt
     5521 ggacaaggtg cttgaagttg gagtgcctaa catcaagctc ttgtgggtag atggttctga
     5581 cttgactgac tactttgaag acggtgagat tgatcgcttg tatctgaact tttcagatcc
     5641 atggccgaaa aaacgccatg aaaagcgtcg tttgacctac aagacctttt tggacacctt
     5701 caaacgtatc ttgcctgaaa atggagaaat tcatttcaaa acggataacc gtggcttgtt
     5761 tgagtacagt ttagtgagct tttctcaata tggcatgaaa ctcaatggtg tctggctaga
     5821 tttgcatgcc agtgattttg aaggcaatgt catgacagaa tacgagcaaa aattctcaaa
     5881 caaggggcaa gttatctacc gagttgaggc agaattttaa gagatagcct aaaattaggc
     5941 tgtacaagtg cttttgcttt acataagttg gcaaacgtgc tatactgata gtaagaatat
     6001 gaaaagtgag gcggggaaat atcttcgcct cttgcttatg aggaggtgga cgcaatcgca
     6061 acaatcgtag aattagtcag agaagttgta gaacctgtca tagaagctcc ttttgaactc
     6121 gtggatatcg agtatggaaa gattggcagt gacatgattc tcagtatttt tgtagataaa
     6181 cccgaaggaa ttaccttgaa cgacacggca gacttgacag aaattatcag tcctgtccta
     6241 gacaccatca agccagatcc cttcccagaa caatatttcc tagaaattac cagtccaggc
     6301 ttggaacgtc ctttgaaaac caaggatgcc gtcgctggag cggttggaaa atacatccat
     6361 gtcggactct accaagccat cgataagcaa aaggtctttg aaggaacttt gttggccttc
     6421 gaagaggacg agttgactat ggaatatatg gacaagacgc ataagaaaac cgtccgaatt
     6481 ccatacagtt tagtatcaaa agcacgttta gcagttaaat tatagaaaaa gaaaggatag
     6541 cttttgagga ttcaaaagtg aagaaaacat gagtaaagaa atgctagagg ccttccgcat
     6601 tttggaagaa gacaagggaa tcaaaaaaga agatatcatc gacgcagtag tagagtcgct
     6661 tcgttccgct tatcgcagac gctatggtca gtcagacagc gtaactattg acttcaacga
     6721 aaaaacaggt gactttacag tttatactgt ccgtgaagtt gttgatgaag tatttgatag
     6781 ccgtttggaa atcagcttga aagatgctct tgccattaat tcagcttatg aacttggaga
     6841 caaaatcaag tttgaagaag caccagctga gtttggtcgt gtagcagccc aatctgccaa
     6901 acaaaccatc atggaaaaaa tgcgcaagca aacacgtgcc atcacttaca atacttacaa
     6961 agaacatgag caagaaatca tgtctggtac agtagaacgc tttgacaacc gctttatcta
     7021 tgtcaacctt ggtagcatcg aagcccaatt gtcaaaacaa gaccaaattc ctggagaagt
     7081 ttttgcttct catgatcgta tcgaagttta tgtttacaag gttgaagaca accctcgtgg
     7141 tgtgaacgtc tttgttagcc gtagccatcc agaaatgatc aaacgtttga tggagcaaga
     7201 aattccagaa gtttatgatg gaactgttga aatcatgagc gtggctcgtg aagcaggtga
     7261 ccgtacgaag gttgctgttc gtagccacaa tccaaacgtg gatgctatcg gtacaatcgt
     7321 tggacgtggt ggtgctaata tcaagaagat tactagcaaa ttccacccag ctcgttacga
     7381 tgctaaaaat gaccgcatgg taccaatcga agaaaatatc gatgttatcg agtgggtagc
     7441 agatccagct gaatttatct acaatgccat cgctcctgct gaggttgacc aagttatctt
     7501 tgatgaaaac gacagcaaac gtgccttggt ggttgttcca gataacaagc tttctcttgc
     7561 cattggtcgt cgtggacaaa acgtgcgctt ggcggctcac ttgactggtt accgtatcga
     7621 tatcaagtct gctagcgaat ttgaagccat ggaagacgct gcttcagtag agttggaagt
     7681 agaaaacgat actgtagaag aataaaagct gctagaggag ggaaagatga aaacaagaaa
     7741 aatccctttg cgcaagtctg ttgtgtctaa cgaagtgatt gataagcgtg atttgctccg
     7801 cattgttaag aacaaggaag gacaagtctt tattgatcct acgggcaagg ccaatggccg
     7861 cggcgcttat atcaaactag acaatgcaga agccctagag gcgaaaaaga agaaggtctt
     7921 taaccgcagc tttagcatgg aagtggaaga aagcttttat gacgagttga tcgcttatgt
     7981 ggatcacaaa gtgaaaagaa gagagttggg acttgaataa gcaaaagata agtaatctct
     8041 tggggcttgc tcagcgagca gggcgcatca tatcgggtga agaattggtg gtcaaggcca
     8101 ttcaagacgg caaggccaag ttggtctttc tagctcatga tgctggaccc aatctggcca
     8161 agaagattca agataaaagt cattattatc aagtagaaat tgtaaccgtg ttttcaacac
     8221 tggaattaag catagcagtc gggaaatcga gaaaggtttt ggctgtaaca gatgctggat
     8281 ttacaaagaa aatgaggtct cttatggaat agaagaggag gacatgattt gtctaagaaa
     8341 agattgtacg aaatcgcaaa agaacttgga aaagaaagta aagaagttgt agcgcgtgca
     8401 aaagagttgg gcttggatgt gaaaagccac tcatcaagtg tggaagaagc tgtcgctgca
     8461 aaaattgctg ccagctttaa gcctgcagct gctccgaaag tagaagcaaa acctgcagca
     8521 ccaaaagtaa gtgcagaaaa gaaagccgaa aaatctgagc cagctaaacc agctgtagct
     8581 aaggaagagg caaaaccggc tgagccagtt gctccgaaaa cagaaaaagt agcagcgaaa
     8641 ccgcaaagcc gtaatttcaa ggctgagcgt gaagcccgtg ctaaagagca ggcagaacga
     8701 cgtaagcaaa ataagggcaa taaccgtgac caacaacaaa acggaaaccg tcagaaaaac
     8761 gacggacgta atggtggaaa acaaggtcaa agcaaccgcg acaatcgtcg ctttaatgac
     8821 caagctaaga agcagcaagg tcagcaaaaa cgtagaaatg agcgccgtca gcaagaggac
     8881 aaacgttcaa atcaagtggc tccacgtatt gactttaaag cccgtgcagc agccctaaaa
     8941 gcagagcaaa atgcagagta cgctcgttca agtgaggaac gcttcaagca gtatcaggct
     9001 gctaaagaag ccttggctca agctaacaaa cgcaaggaac cagaggaaat ctttgaagaa
     9061 gcggctaagt tagctgaaca agcacagcaa gttcaagcag tggttgaagt cgtccctgag
     9121 aaaaaagaac ctgcagtgga tacacgtcgt aaaaaacaag ctcgaccaga caaaaatcgt
     9181 gacgattatg atcatgaaga agatggtcct agaaaacaac aaaagaatcg aagtagtcaa
     9241 aatcaagtga gaaatcaaaa gaatagtaac tggaataaca acaaaaagaa caaaaaaggc
     9301 aataacaaga acaaccgtaa tcagactcca aaacctgtta cggagcgtaa attccatgaa
     9361 ttgccaacag aatttgaata tacagatggt atgaccgttg cggaaatcgc aaaacgtatc
     9421 aaacgtgaac cagctgaaat tgttaagaaa cttttcatga tgggtgtcat ggccacacaa
     9481 aaccaatcct tggatgggga aacaattgaa ctcctcatgg tggattacgg tatcgaagcc
     9541 aaacaaaagg ttgaagtgga taatgctgac atcgaacgtt tctttgtcga agatggttat
     9601 ctcaatgaag atgaattggt tgagcgtcca ccagttgtta ctatcatggg acacgttgac
     9661 cacggtaaaa caaccctttt ggatactctt cgtaactcac gtgttgcgac aggtgaagca
     9721 ggtggtatta ctcagcatat cggtgcctac caaatcgtgg aaaatggtaa gaagattacc
     9781 ttccttgata caccaggaca cgcggccttt acatcaatgc gtgcgcgtgg tgcttctgtt
     9841 accgatatta cgatcttggt cgtagcggca gatgacgggg ttatgcctca gactattgaa
     9901 gccatcaacc actcaaaagc agctaacgtt ccaatcatcg tagctattaa caagattgat
     9961 aaaccaggtg ctaacccaga acgcgttatc ggtgaattgg cagagcatgg tgtgatgtca
    10021 accgcttggg gtggagattc tgaatttgtt gaaatctcgg ctaaattcaa ccaaaatatc
    10081 gaagaattgt tggaaacagt ccttcttgtg gctgaaatcc aagaactcaa agcagaccca
    10141 acagttcgtg cgatcggtac ggttatcgaa gcgcgcttgg ataaaggaaa aggtgcggtc
    10201 gcaacccttc ttgtacaaca aggtaccttg aatgttcaag acccaatcgt tgtcggaaat
    10261 accttcggtc gtgtccgtgc tatgaccaac gaccttggtc gtcgtgttaa agttgctgga
    10321 ccatcaacac cagtctctat cacaggtttg aacgaagcac cgatggcggg tgaccacttt
    10381 gccgtttacg aggatgaaaa atctgcgcgt gcagcaggtg aagagcgtgc caaacgtgcc
    10441 ctcatgaaac aacgtcaagc tacccaacgt gttagccttg aaaacctctt tgataccctt
    10501 aaagctgggg aactcaaatc tgttaatgtt atcatcaagg ccgatgtaca aggttctgtt
    10561 gaagcccttt ctgcctcact tcaaaagatt gacgtggaag gtgtcaaagt gactatcgtc
    10621 cactcagcgg tcggtgctat caacgaatca gatgtgaccc ttgccgaagc ttcaaatgcc
    10681 tttatcgttg gtttcaacgt acgccctaca ccacaagctc gtcaacaagc agaagctgac
    10741 gatgtggaaa tccgtcttca cagcattatc tacaaggtta tcgaagagat ggaagaagct
    10801 atgaaaggga tgcttgatcc agaatttgaa gaaaaagtta ttggtgaagc ggttatccgt
    10861 gaaaccttca aggtgtctaa agtcggaact atcggtggat ttatggttat caacggtaag
    10921 gttgcccgtg actctaaagt ccgtgttatc cgtgatggtg tcgttatcta tgatggtgaa
    10981 ctcgcaagct tgaaacacta taaagacgac gtgaaagaag tgacaaacgg tcgtgaaggt
    11041 ggattgatga tcgacggcta caatgatatt aagatggatg atgtgattga ggcgtatgtc
    11101 atggaagaaa tcaagagata agattttttg ctcctttctt aggtggtgag ggacgcaagc
    11161 aaaccgatgg tttcattgct tatttttgag cctagggtct caaaaatccc ctgtgatggg
    11221 actgataaat cagttccatc actttcacca cggcgaaaga agcagatgac ttcaaattga
    11281 acttcgtttc aatttaaact gaaaatcaag aagtttaaaa tagctaggtc tgctggccta
    11341 gcttttggtt caaagtagag aaaggaatat catggcaaat catttccgta cggatcgtgt
    11401 gggcatggaa atcaagcgtg aagtcaatga gattttgcaa aagaaagtcc gtgatccacg
    11461 tgtccaaggt gtgaccatca cagatgttca gatgctgggt gacttgtctg ttgccaaggt
    11521 ttattacacc attttgagta accttgcttc ggataaccaa aaagcccaaa tcgggcttga
    11581 aaaagcaact ggtaccatca aacgtgaact tggtcgcaat ttgaaattgt acaaaatccc
    11641 agatttgacc ttcgtcaaag acgagtccat cgagtatgga aacaagattg acgagatgct
    11701 acgcaatctg gataagaact aaagaagagg ggttgcccct cttttttggt ggaggaaaat
    11761 aggttgaatt tgaaatggaa aaatattctt ttaaaatata ttgaaactag aatagtacgc
    11821 ctttacttct aaaaaattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc
    11881 ttgtttcatt ttactataaa aaaggggttc tgtatttttt aatgttatct aattagaaaa
    11941 tgcttttttt gtaggaaata taatatgata aggtgtaaaa aagaaataag gagtttgtat
    12001 atggctgaac aagacttagc tatgcaagta ttacaacaag tggtaaaact accagttgtt
    12061 aaggttgata gttcgaaatt tttagtggat aagttttcca aagaattggg tccacaggac
    12121 attcctacct tattggaaca aggtccaacg tctctcttat ctcaagaaat cttagatcga
    12181 gtagctaatg cttgtattcg ggacaatgta ttattagcga gtgggacttc tgttttggca
    12241 ggattacctg gagggcttgc tatggcaatt accattccag ctgatgtggc tcaattttat
    12301 gctttctctc tgaaattggc tcaagaatta ggttatattt atggttatga ggatctttgg
    12361 gtttcacgag aggagttgag tgaagatgct caaaataccc tcttgcttta tctaggcgta
    12421 atgttagggg tgaatggaac tgctgctttg ctacgtgctg gtggtataac aattgccaaa
    12481 caggtaatga aaacagtgcc taataaagct ttaacaaaga cgctttggta ccctattttg
    12541 aaaaaagtct taaaaatatt tggtgtgaat cttaccaagg gagggttggc caaaggaatg
    12601 gggaaattta ttcctatctt gggtggtatc atttcaggtg gtttaacctt tgcaactatg
    12661 aaaccaatgg gggaaagctt gcagaaagaa ttatccaagc tagtcaacta tagtgaagtt
    12721 caatatcaag aagatgttga aacaatccga aaagaggctg aaatcatcga aggagagtaa
    12781 tatgggactc ataaaaactc tagctaaaat ttatggtaat tactttttga ccgtgcaagg
    12841 tgtaaaagtg atgaaaacga taaagaaagc tgaccatgtc gttgttggtc tgggcaaact
    12901 ttttattgca gataagctaa tggatacggc tcggtggctc attaagccag aggagagaga
    12961 atgaaatttt tttggtcttc ttgctattct ttttatcaaa ccgattattg ggattgtgaa
    13021 attcttttgg atgatcattt cttttgcagt tcaattgctg ttttacaaga tattggattg
    13081 gttctttaaa cttatctaga tggtaatcca agtcgcagaa aactagcagg aaccccactg
    13141 ctagtttttt aatctctctc catatggtat aatataagca gtaaaatcat tttatactct
    13201 tcgaaaatct cttcaaacca cgtcagcttc accttgcagt atatatgtta ctgacttcgt
    13261 cagttctatc cacaacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatccacaa
    13321 cctcaaaaca ctgttttaag caacctgcgg ctagcttcct agtttgctct ttgattttca
    13381 ttgagtatta gaacatacaa tggaggtcgt catggacaat atcatcgatg tgtcaattcc
    13441 tgttgcagaa gtggtggaca agcatccaga agtcttggaa attctagtgg agttgggttt
    13501 taaacccctt gccaatccct taatgcgcaa tacagttggt cgtaaagtat cacttaaaca
    13561 gggttctaag ctagcaggaa ctcctatgga caagattgta cgcacactgg aagcgaatgg
    13621 ctacgaagtg attggattag actaatgaca gatgaacgga ttcatgtcct acgggatatt
    13681 ttgttagaat tgcacaatgg cgcctctcct gagtcggttc aagatcgctt tgatgcgacc
    13741 tttacgggcg tgtcagccat cgagatttcc cttatggagc acgagctgat gaactcggat
    13801 tcgggcgtca cttttgaaga tgttatggaa ctctgtgatg tccatgccaa tctttttaaa
    13861 aatgctatca aaggtgtcga agtttcagat actgagcatc caggtcaccc agttcgtgtc
    13921 ttcaaagaag aaaatctggc tctccgtgcg gccttgattc gcattcgtag attgttagat
    13981 acctatgagt ctatggaaga cgaggaaatg ctggcggaga tgcgtaaggg tttggtgcgt
    14041 cagatgggac ttgtgggtca atttgacatc cattaccaac gtaaggaaga actcttcttt
    14101 cctatcatgg agcgctatgg acacgattca cctcccaaag ttatgtgggg agtggatgat
    14161 cagattaggg aactctttca aacagctcta acgacagcca agtcactacc agaagtgtca
    14221 attagcagtg taaaggaagc ttttgaagct tttgcgacag agtttgaaag tatgattttc
    14281 aaggaagagt ccatcctgct catgattctc cttgagtctt ttactcagga tgactggctt
    14341 cagattgcgg aggagagtga tgcctatggc tatgccatca tccgtccgtc agagaaatgg
    14401 gtgccagaac gacagatctt tattgaggaa aagattgcag aggagcctgt acagctagat
    14461 acggcagaag gtcaagttca acaagtcata gatacgccag aaggccattt taccattacc
    14521 tttaccccta aggaaaagga agctgtgctg gaccgccata gtcaacaggc ttttggtaat
    14581 ggctatcttt cagtcgagca ggccaatctc atcctcaatc atctccctat ggagattacc
    14641 tttgtcaata aagaagatat tttccagtat tacaatgaca atacgccagc tgatgagatg
    14701 attttcaaac ggacgccgtc ccaagtcggg cgcaatgtcg aactctgtca tccgcctaag
    14761 tatttggaca aggtcaaggc tatcatgaag ggacttcgtg aggggaccaa ggacaagtat
    14821 gaaatgtggt tcaagtctga gtcgcgagga aagtttgtcc acatcaccta tgctgcagta
    14881 cacgatgaaa acggagaatt ccaaggtgta ctggaatacg ttcaggatat ccagccctac
    14941 cgtgagattg atacggacta tttccgtgga ttagaataag gagaaaaaat gagttacgaa
    15001 caagaattta tgaaggaatt tgaagcttgg gtcaataccc aaatcatgat taacgacatg
    15061 gcgcacaagg aaagccaaaa agtttacgaa gaagaccagg acgagcgtgc caaagatgcc
    15121 atgattcgct acgagagtcg cttggatgct tatcagttct tgcttggtaa gtttgaaaac
    15181 ttcaaagtag gcaagggatt ccatgatttg ccagaaggct tgtttggtga gcgaaattat
    15241 taaacgagaa agattcttga tttttcacta aaatcttgat agaatgttta tgttaaatcc
    15301 ttgtcagagc agggattttt tattgaaagg attttatcat gtcaaagaaa ctcaatcgta
    15361 aaaaacaatt acgaaatggc ctccgtcgcg caggtgcctt ttcaagtact gtgactaagg
    15421 ttgtagatga gacaaaaaaa gtcgtgaagc gtgcagaaca gtcagcaagc gcagctggta
    15481 aggctgtttc taaaaaagtt gaacaagcag tagaagctac caaagagcaa gctcaaaaag
    15541 tagctaattc tgtagaagat tttgcagcaa atttgggtgg acttccactt gatcgtgcca
    15601 agactttcta tgatgaagga atcaagtctg cttcagattt caaaaactgg actgaaaaag
    15661 aactccttgc cttgaaagga atcggcccag ctaccatcaa gaaattgaaa gaaaatggca
    15721 tcaagttcaa gtaatttttc ttgagccttg catttccgaa aaaatcttgc tacaatagag
    15781 ccattagagg tgttttgaat cccacatttt acagaaagtg gcggcgctga gaagtccaca
    15841 aatgtgtcaa aactggttgc taatggatga aaaattgaaa taaaagtgtc tttttgcttt
    15901 aaagacgaga gttgcgggca actgcccgaa attgggtggt accgcggata aacacattcg
    15961 tccctgtcat gtagatggca gggcttttct tttacgtctt agtcaaaggg ggttgttatg
    16021 aagcaatctt ttaacacaag taaactatat tatggttttc ctgtttttat tttaggatat
    16081 caagattaga attttggcta taacatcacg acctgtagtt cctcttatag tctaggagat
    16141 tgggttgtga ttggagtcgt tgcgagagag aatgccgcag agcagatcaa acagtatcaa
    16201 aaatttactg tgaatatttc tgatgaaact tctatgcttg cgatggagca ggctggtttt
    16261 atcagtcatc aggagaaatt ggaacgtttg ggagtgcatt atgaaatttc tgaacgaact
    16321 cagatttcta ttttagacgc ctgtccactt gttttagatt gtcgggtaga taggattgtt
    16381 gaggaagacg gtatttgcca catctttgcc aagattcttg agcgacttgt tgccccagaa
    16441 ttcctggatg aaaagggaca ttttaaaaat caactgtttg ccccaaccta tttcatggga
    16501 gatggatatc agcgcgttta tcgctatctg gacaagcgtg tagatatgaa gggcagtttc
    16561 atcaaaaaag cgaggaagaa ggatggcaag aactgagctg tcagataaaa tcgaaacaga
    16621 acgtctcgtt ttacgagtcc gtactgtggc ggatgctgag gatatccatg cctacgctag
    16681 tttgccagag gtcgcctatc cagcaggttt tcctccagtc aagaccttgg aagatgagat
    16741 ttattatctg gagcacattc ttccggagcg taatcaaaag gaaaatctcc cagcaggcta
    16801 cgggattgtc gtcaaaggaa cggataagat cgctggctct gtcgatttca accatcgcta
    16861 tggggacgat gtgctagagc ttggctatac cttacactca gactactggg gtcgaggtta
    16921 tgttccagaa gctgcgcgtg ccttgattga cttagccttt aaagatttgg gtcttcacaa
    16981 gattgaacta acttgctttg gatataacct tcaaagtaaa cgagtcgcgg aaaagcttgg
    17041 ctttaccctc gaagctcgca taagagaccg caaagatgct caaggcaact gctgtgatga
    17101 tttaagatat gccttgctga agagtgaatg ggaggaataa gatgagcgat gtaaaagagg
    17161 aagtaagctc tctatctgag aagcagttac gccaaattga tgttgagtat gctgaattaa
    17221 atgatagtga tattatcgag cgattagctt atttagaaat taacaacaat gaaaaacgta
    17281 ttgtcatctc tgatattgaa ccaaccaagg aaataatgtc agtctcggac caaatttttg
    17341 agattcaaaa gaattttcaa aaaatcaaga atatgttcga attgtttatt tcagatgtct
    17401 cggatttttt aagtattaaa aataaacttg agtcaaagga attagaaatt gaagaggcag
    17461 atgtaaatcg tttcatgatt catttattat ccagtggaaa attgtttgtc gattttaacg
    17521 aaaaccaaat taaacaaaaa tattccaaag atagtgagga gtttgattgt atacatggtt
    17581 ttgcaagcta tcagtatgat atcaatttta cttatcgatt ttgccattct ttgagaaatt
    17641 attcgcaaca tactgactta ccaataaatg aggtaaaagc tgttagccca gatgatgaga
    17701 ctgttataat agatttctat attgatttag attatctttt aaattccaac tttaaatgga
    17761 aaaaattgaa aggggagttg ataaaactaa accaagaaac atctaagatt gacgctatag
    17821 ctctagtgaa agaatacttt aatgctctta cagagctcta tggtaattat aataagttgt
    17881 t
//
LOCUS       DAVMAF010000035        17818 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMAF010000035 DAVMAF010000000
VERSION     DAVMAF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.035"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            49..1503
                     /locus_tag="VYO97_001234"
     CDS             49..1503
                     /locus_tag="VYO97_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1754027.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRFQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            1484..2449
                     /locus_tag="VYO97_001235"
     CDS             1484..2449
                     /locus_tag="VYO97_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1754028.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(2484..3758)
                     /locus_tag="VYO97_001236"
     CDS             complement(2484..3758)
                     /locus_tag="VYO97_001236"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1754029.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3758..4930)
                     /locus_tag="VYO97_001237"
     CDS             complement(3758..4930)
                     /locus_tag="VYO97_001237"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1754030.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(5061..5309)
                     /locus_tag="VYO97_001238"
     CDS             complement(5061..5309)
                     /locus_tag="VYO97_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754031.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(5377..6003)
                     /locus_tag="VYO97_001239"
                     /pseudo
     CDS             complement(5377..6003)
                     /locus_tag="VYO97_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6065..6514)
                     /locus_tag="VYO97_001240"
     CDS             complement(6065..6514)
                     /locus_tag="VYO97_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1754032.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(6516..7274)
                     /locus_tag="VYO97_001241"
     CDS             complement(6516..7274)
                     /locus_tag="VYO97_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1754033.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(7389..9368)
                     /gene="pknB"
                     /locus_tag="VYO97_001242"
     CDS             complement(7389..9368)
                     /gene="pknB"
                     /locus_tag="VYO97_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1754034.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(9365..10105)
                     /locus_tag="VYO97_001243"
     CDS             complement(9365..10105)
                     /locus_tag="VYO97_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1754035.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(10120..11433)
                     /gene="rsmB"
                     /locus_tag="VYO97_001244"
     CDS             complement(10120..11433)
                     /gene="rsmB"
                     /locus_tag="VYO97_001244"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1754036.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVTKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(11426..12361)
                     /locus_tag="VYO97_001245"
     CDS             complement(11426..12361)
                     /locus_tag="VYO97_001245"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1754037.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMIFRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEDQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(12374..14770)
                     /locus_tag="VYO97_001246"
     CDS             complement(12374..14770)
                     /locus_tag="VYO97_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1754038.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRSGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(14836..15150)
                     /locus_tag="VYO97_001247"
     CDS             complement(14836..15150)
                     /locus_tag="VYO97_001247"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1754039.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(15175..15801)
                     /gene="gmk"
                     /locus_tag="VYO97_001248"
     CDS             complement(15175..15801)
                     /gene="gmk"
                     /locus_tag="VYO97_001248"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1754040.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(15931..17544)
                     /locus_tag="VYO97_001249"
     CDS             complement(15931..17544)
                     /locus_tag="VYO97_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1754041.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILVQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         4840 a         4190 c         3570 g         5218 t
ORIGIN      
        1 tatcgaaaac agttactatt aaaagtgata agatttttgg aggaatgaat ggaatggaca
       61 actgagcgtc gttacagact ttatcaagat tggacgcaag aagaaattca acatataaag
      121 gaaaatatgg cacaatctcc atggcatact cattaccatg ttgagccaaa aacaggactt
      181 ctcaacgacc caaatggctt ttcttacttc gatggcaagt ggatcctctt ttaccagaat
      241 tttccttttg gtgcagccca cggtttaaaa tcttgggcac agctagaaag tgatgatttg
      301 gttcacttta gagaaactgg agtcaaagtt ttgccagata ctccattaga tagtcacggt
      361 gcctactctg gttctgccat gcaatttggc gataacttat tcctatttta tacaggaaat
      421 gttcgcgatg aaaactggat ccgtcaccca taccaaatcg gtgctttgat ggacaaggag
      481 ggtaagatta caaagattga caagatcttg attgaccagc cagcagactc tactgaccac
      541 ttccgcgatc cacaaatttt taactttcag ggtcaatatt atgccattgt cggcggacaa
      601 gacttggaga aaaaaggttt cgttcgtctc tacaaggctg tcaataacga ctacacaaac
      661 tggcaagcag ttggcgacct tgactttgct aacgaccgta ctgcctacat gatggaatgt
      721 cctaatctgg tctttgtaga ggaacagcct gtccttctct actgtccaca aggattggat
      781 aagaaagttc tagactacga taatatcttt ccaaatatgt ataagattgg ggcttccttt
      841 gaccctaaaa atgccaaaat ggtagatgtg tctcaacttc aaaacatgga ttacggtttc
      901 gaagcctatg caactcaagc cttcaacgct cctgatgggc gtgctctagc agttagctgg
      961 cttggtttgc cagatgtttc ttacccatct gaccgttttg accaccaagg aaccttctct
     1021 ttggtcaagg aactcactat caaagacgac aagctctacc agtatccagt cgctgctatt
     1081 aaggaccttc gtgcttctga agaagccttc tcaaaccgtt tccaaaccaa gaacacttac
     1141 gaacttgaac tcaacttgga agctaatagc cagagcgaga ttgtcttact tgctgataaa
     1201 gaaggtaagg gactttcaat caactttgac cttgtaaacg gtcaagtgac agtggatcgt
     1261 agccaggctg gagaacagta tgcccaagaa tttgggacaa ctcgttcttg ccctatcgag
     1321 aatcaggcta ctactgctac aatcttcatc gataactctg tctttgaaat tttcatcaat
     1381 aaaggagaaa aagtattttc tggtcgtgtc ttcccacatg cggaccaaaa tggtatcctg
     1441 atcaaatctg gaaacccaac tggaacttac tatgaattag attatggtcg caaaactaac
     1501 tgatgtcgcc aaacttgcag gcgtcagtcc tactaccgtt tctcgggtta tcaataaaaa
     1561 agggtatcta tctgagaaaa ccatccaaaa agtcaatgaa gccatgcgag aattgggcta
     1621 taaacccaac aacctggctc gtagtctgca aggaaaatca gctaagttaa tcggcttgat
     1681 tttccccaat atttccaatg ttttctatgc agaattgatt gataaattgg aacaccaact
     1741 cttcaaaaat ggttacaaga ccatcatctg caacagtgaa catgattctg agaaggaacg
     1801 tgaatacatc gaaatgttgg aagccaatca ggtggacggc atcatttctg gtagtcacaa
     1861 cctaggaatc gaagactaca atcgtgtgac agcgccgatt atttcctttg accgaaacct
     1921 atcgccagac atccctgtcg tctcctctga caactatgct ggtggggttc ttgctgccca
     1981 aaccttggtc aagacaggtg cccagtctat catcatgatt acagggaatg acaattccaa
     2041 ttcgccaacc ggactgcgcc acgctggttt tgcatccgta ctcccaaaag ctcctattat
     2101 caatgtttcc agtgactttt ctcccgtcag aaaagaaatg gaaatcaaga atatcttgac
     2161 ccgggaaaaa ccagatgcca tttttgcttc ggatgatttg acagctattc tggtcattaa
     2221 aatcgctcaa gaattgggca tttctgtccc aaaagagctc aaggtcatcg gctatgatgg
     2281 gacctacttt atcgaaaatt actaccctca attggctact atcaagcaac ctttggaaga
     2341 gattgcttgt ctcactattg atcttctctt gcaaaagatt gaaggcaagg aagtcgccac
     2401 aactggttac ttcttaccag ttacgctatt accaggaaaa agtatttaaa cacaagaaaa
     2461 ctcagaccga ttcgtctgag tttttatgat cttaaatttt cgagatagcg ctgggctgtc
     2521 tctaggttaa aggttttatc tgagatgagg cgctctacta ggggagcaac ttcagattca
     2581 ctagccccag ctaggagagc tagggatttg gcctgtagtt tcatgtggcc ttgctggatg
     2641 cccgtactta ccaaggcttt gagggctgca aaattttgag caagaccgat ggacacgata
     2701 atctgggcta attctctggc agaaggattt cctagtagat catgactgag agctacacgt
     2761 gggttgaggc cgatagagcc acccttagtc gctacaggca tgggcagggt catctcaccg
     2821 accaattctt ctctttcaag gtccagcgtc cagcagctaa gaccttgata gcgtccatct
     2881 cgactggcaa aggcatgggc cccagcttcg atggcacgcc agtcattacc agtggcaatc
     2941 aaaatcgcat caataccatt aaaaattcct ttattatgag tagcagctcg gtaaggatca
     3001 gcctgcgcaa actgactagc caacgcaatt ttctccgcaa tctctcgtcc ttgatccttt
     3061 tggcggctca agtagcgaaa ggcgatgcga cagcttgcag tcaccagaga atcagtcgcg
     3121 tagttggaca ggattcccat gagactctgt ccctgactga gttcttctaa gactggtttc
     3181 aaggcttcca gcatggtgtt gagcatattg gcacccatgg cttcctgggt atcgacatga
     3241 atataaacaa cgagaaagtc tggttcgcct tttatctgct cgacatgcag atcacgcgcc
     3301 ccacccccac gtttaacgat agaaggatag gcttgattgg caagctccaa gagctccgct
     3361 ttcttgctgg caatcttctc ttgcgctagt ttaggattag caatttgata aagggctacc
     3421 tgcccaatca tctggcgctg atggacttgt gcagtaaaac cacctgcacg cttgatgatt
     3481 ttgctggcat agctggccgc cgcaaccaca gagggttctt ctgtcacata gggaacggtg
     3541 tattcctgac cgttgacaag tacctccgga accagcgaat aaggcagaga aaaagttccc
     3601 accacattct cactcagctg gtctgccaca gtcacactca tctgttcatc cttctccaga
     3661 ctagcttgtc tctcaggact aaggagcgcc tgagctttta gcagctcgag gcgctcttgg
     3721 tatgattttt tagaaaatcc attccaactt atcttcatta tttttcaacc ttgctataac
     3781 ggcgttggtg gtcaagaatt tcaaccaagg caaaatcttg attttcatag ccagcaaact
     3841 gggcagagtt tgtttcatcc aagtttactt cctcaaaaaa gaccttttca tagtctgcaa
     3901 cggatagggc agttcgttgg ttgagcttgt tcaagcggtc tttatccaaa taagcttcat
     3961 atccttcaac caattcacca ctgaagaact cagccacagc tccacttccg taactataaa
     4021 gggcgatttt atccccagct ttcaagctat ctgtattttc caagagagac aaaagtccaa
     4081 ggaaaagtga acctgtgtag atattcccca ccttttgact gtagagaata gactggtcaa
     4141 aatgcttttg taagaggtct tttttctctt gaggcaggct cttatccatg atttttttca
     4201 agccttttag cgctaattta ggataaggca agtggaaaca aacagcagca aaatcatcca
     4261 aagtaagctg gtagcgtttt tgatattcaa gccaagtcgt tttcaaacta tccaagtatt
     4321 gttgggtaga atagacacca tttacataag gagttgtcga gtaatttggt cgccagaaat
     4381 ccatgatgtc acgggtctga gctacattgt cattattaaa ggccatcatg cgtggatttt
     4441 gtgtaatcaa catagctaca cttccagcac cttgagttgg ttctcccgga gtttcaatac
     4501 catatttggc aatatcactg gcaatgacca ataccttgga ctccggagaa ttttccacat
     4561 gcaatttggc ataatggagg gcagctgtcg ccccatagca ggcttcttta atctcgaaac
     4621 tacgagcaaa gggctggatg cccagcaagc catgcacaaa gacggccgca gccttactct
     4681 ggtcaattcc tgactcggta gccacaatga ccatgtcaac ttcttgtctt tcttgctcag
     4741 ttaaaataga gtcactagca ctggccgcca aggtcacgat gtcctcagtt aggggcgcaa
     4801 tactcaattc cttgagtaag agtcctttac ttaatttttc agggtcaatt cccctcgctt
     4861 ctgctaagtc ttgtaatttc aagacatatt gactggtcgc aaaaccaatc ttatcaatac
     4921 cgattgtcat atttacctct gttttatcat tcatgtaaaa aatcgttcta tactatttta
     4981 tcacaaatgg cagtaaaaga gagaaaaaag acttgattca ccaaatcaag cctcttattg
     5041 gtcatcattt taaagaatga ttagttgcta gagagttcac cgatataagt agctttataa
     5101 gctccattca cagttatcag ctcctggagg atcaaatttc ctgagtaagt ccttcccatc
     5161 tcatctacaa atttttgata aaactgactg gtcggaattt ctctgacatc cttatcaaat
     5221 gtcttgtcaa gtgttttact aaccttctca gcaatcaatt gatgctcttg ccatccactt
     5281 tgaaactctg agcccgaact agaaaccatg actgggataa acaacaaggt cagtagactt
     5341 acagacaata aggaaaatag tagacttcct gcaaaactag aatcctagtt catgattgat
     5401 aataccaaca atcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt
     5461 tgaaaacatt ttaaacgttt ttactttggc aaagatgttc tcaactttgc ttctctcctt
     5521 agatagcgca tggttatagg ctttatcttc agctattagc ggcttgaggt tgctggattt
     5581 acgtggagtt tgtgcttgag gatatatctt catgggccct tgataaccac tgtcagccaa
     5641 gattttacca gcttgtccga tatttctgcg actcattttg aacaacttca tatcgtggca
     5701 atagttcacc gcaatattca aagaaacaat tctttcttgg ctggtgacaa tcgcttgagg
     5761 ttccatagaa tgacatttct attttaccag aataattggc tagttgattt ttttaggacg
     5821 atttattttt acctctgttg catccacaat caccgtattc tcagcactaa gatgagtttt
     5881 tgaaatcgta acaccacttt gaacaagagt tacttcaacc cattggctcc gacggattaa
     5941 gttgctttcg tggataccaa aatcagccgc aatttcttca taagttcgat attctcgcac
     6001 atatagaaag cgttgtcaat ctatttgtct catttttcag aaaattcttt tatttctgta
     6061 aagtctacga tactcgatgt gtttttatat aatgatagag tctgagaatc actgttccgc
     6121 tagccattcc aatagagatt accagagcca acatgacaac caaggtcgca cttgccagtg
     6181 ctttattata gtcccctgtc acaaaaaagg cagttgttcg gtaggagaga taacctggaa
     6241 ccagcggtgc caaaatggcc aagataaaga ccacagcagg tgtcttataa agaatactta
     6301 aaatctggct gacacaagaa ccaataatgg ctgcaatgaa ggtagctaca atgacattgg
     6361 tcggttcctt gagcaagaga tagattagcc agacagtcat gcccaaaatc cctccaggta
     6421 agagcataga ccgttgcaca ttgagtacga ttaaaaaagt gataatggca agaaaacttg
     6481 ctactgcttg taataaaaag gttgttagtg tcatattagt tcatcaatac caaggcgaca
     6541 gaagttcctg cccctaaagc gagggtaatg agcagggatt caaacatctt actcatacca
     6601 gagtttatgt ggttggtcat aatatcacgg accgcattgg tcaaggcaat acctggtaca
     6661 aacggcatga ccgcaccagc tataatcaaa tctgccgttg aaggaaaacc tgtgtagcga
     6721 gcccaaaact gggcaattat cccaaagaca aaggctccag caaaggctgt cacaaaggga
     6781 attcggataa atttttccac atagagggaa aaggcaaaac caaataaggt cgccactcct
     6841 gccccaagtg cgtcgtagat atttccgcta aacataactg aaaagaaagg agcactaaag
     6901 gtcgcagcca gagttacctg caacttagta tagggaaggg gttgagcttg caaggccgtc
     6961 aattgcttaa aggctgtttc taagtcaatc tgccccccaa ctagctgacg agaaatctgg
     7021 ttcacatcgc agactttttc gatgttataa gaagaggagg tcacgcgctt catgcgcgaa
     7081 atattggtat tttcaataga gaaaaagata gcggcaggca tggcaaggac attgcaatcc
     7141 acaatcccct gcgaatgcgc gattcgaatc atggtatctt ctacacgatg gatttctgag
     7201 ccacttttaa ggagaatagt ccccgctagc ataatcacat caatgacggc atttaattct
     7261 tttgattctt ccatgctttc ctccttttat caactccctc tattctatca caaatctgga
     7321 ctcaaaaaaa atctttgcca tgaaatcatg acaaagattg attactcatt ttgattatcc
     7381 atctgctttt aaggagtagc tgaagttgtt ttaggtttgt agattgaaat cttgactcta
     7441 gtcttattta ggtctacctt ttcacctgct ctaggacttt gttcaacaac catgccttct
     7501 acactacctg caggcgctgt cgtcacttct acaacttcta tattagcttc cttaatccca
     7561 acaatttgaa tcaaattgtt cttagtaaac tccaagctgg aaccaatgta actcggcatg
     7621 gcaacacttg taactttttt agctactgtc aagacaattt gagtaggttt actcacatca
     7681 taagtcgttc cggcacctgg actttgtttc ataatcgttc ctggttcgct ttcgctggac
     7741 tcttcttcct ctatcttaat caaattctca ggaaccttct tctgcttgag ttctgagatt
     7801 acttctgtag agttccgtcc aatatagttc cctaattgaa tcgtcgtagc ttttttagct
     7861 actgtcaaaa caatttgagt tgccttgctc aagtcatagg tcgtaccttc tggtagactt
     7921 tgcttcagga ccgttccagc ctcactctca ttcgactctt cttcctcaat tttaatcaaa
     7981 ttatctggaa cttttttctc ttttaattcc gcgataacat cagaagattt ccggccgaca
     8041 taattactaa tttggaagga ttgtttgcct gatgagacaa ccagattgat tttcgttcct
     8101 tcttttcgac cagttccagc gccaggatct gtacggataa tccgcccttc ttccaccttt
     8161 tcactagcct ctgtcttctc ctcaccaatc tcaaaattgg cttttttgag cgttgccttg
     8221 gcctctgcaa ctgtctgacc tgccacatct ggaatggcaa tggttgcagg agttctggat
     8281 agtatccaaa taagagaagc tgccaccaat acaaggctgg ccaacaaaat caggtaacgc
     8341 atcttaaatc tatgtttttt cggtgcttgt ggttggtaag tttcctctgt cacagcctgg
     8401 cttgggtttt tgattgattt gtgttctgtc tgcgcttgaa ccttaggaat agatgtcaag
     8461 gtactctgag aaaccttcgg caaggtcttg gtatctgcct tgctcgtttc atcaaagatt
     8521 aacttacttt catttctacg attgtaggac aagctactag acaagtccac atacatctct
     8581 gaaaccgagc ggtagcgatt ggtcaacttt ttagcagttg ccttgataat aacattttct
     8641 aaagcctgag gtacagatgg attttctgca ataacggacg gcaggggttt ctggaaatgc
     8701 tggagggcaa tggtcaccgc gctatccccg tcataaggga tatggcctgt caacatctca
     8761 tagaaaataa tccccatggc atagatatca ctctgcacag tcgccttcga accacgcgcc
     8821 tgctctggtg acaagtaatg aactgagccc aacatcgagt tagtctgggt cagacttgtc
     8881 tctgcaaagg ctacagcaat cccaaagtct gtgaccttgg ccgtcccatc tggtgtcaaa
     8941 aggatatttt gaggtttcaa gtccctgtga acaattcctc gagtatgggc caagcgcata
     9001 gccaagagaa tttgtcccat gatacggact gcttcttcat tagaaagagg ataatgttcc
     9061 ttgatatagc gtttgaggtc tagtccagca acatactcca ttgcaagata ctgttgaccg
     9121 tcttcctcgc caatatctgt tatccgaacg atatgaggat ggtctagatc tgccatagct
     9181 ctcgcttcac gctgaaaacg agctacagct atcgggtccg tctggtagtt ggtcctcaga
     9241 accttcactg ccacttcttc cccatctaag attaagtctt tggctaggta gacatccgcc
     9301 atgcctcctc gaccaatctg tttgacaatc cgatagcgtc cggcaaaaat cttgccgatt
     9361 tggatcattc ttcatcctcc tcgttcatag aaacaagggc aaccgtaatg ttgtctaaac
     9421 ctcctgcatt gttagcaaaa cgaacaagtg tctccgtttt atctgctaaa ggaatatcac
     9481 tggttacaat atcacgaatc tcactgcctg aaatcatgtt ggtcaagccg tcactattga
     9541 gcaagagata gtcacctgac tcaaggataa ctgtcccaaa atcaggctga atttcatctt
     9601 tttgcccaat agactgggtg ataatatttt tttgcggatg agcttctgcc tcttctggtg
     9661 tcaattgacc agccttgagc aattcattaa ccaaggaatg atcgctcgtc aactgatggt
     9721 attcttctcc acgaatcaag ccgatacgcg aatcaccaat atgagcatag atagcctgat
     9781 tatcaataat agcaaggact tccaaagtag ttcccatgcc tctgtaagct tcatcctgac
     9841 caagctggtg aatcttttga ttttcaattt ctaggtaatg ggcgaaccat tcacgcactt
     9901 cattgactgt atcgatctgg gtatcaaccc aagctacacc caggtctgtg accgccattt
     9961 cactagcgat attccctgca cgatgacctc ccatcccatc agctaaaata atcatggtac
    10021 gtccagctct attgacatag tggttgacat agtcttggtt atttgttcgt ttctgaccaa
    10081 catctgttaa taatgaaatt tccatgtgtc agttccttcc taatccgata tcttgcgaaa
    10141 ttgactgatg aagaatccat cacttccata caattcaggt gtaatgagga tacagccgtc
    10201 tttcatgata tccttacatt catgttctag ttttacctgc tcgaactcgg gatgactctc
    10261 taaaaaggcc ttaacgactt gaaaattctc ctctgagacg atagtgcagg tgctataagt
    10321 tattatacca cctttgccta gtatttgaca aacactacct aatatttcta actgaatttc
    10381 ctgcaaggac gcgaaatctg ccgtttcttt attgtatttg atatctggtt ttcggcgcaa
    10441 aagaccgatt cctgaacaag gagcatccac caaaatctta tcaaaggaat cctggtcaaa
    10501 aaactcatgc acctttctgg catccaattt ttgagtttga acccgatctg caactcccag
    10561 acgttgggca ttttcttgaa ttaaatccaa cttgtggtcg tacaagtcca gagcagtaac
    10621 ctgacctgtc gtaagataag aggctatatg ggctgttttc ccacctggag ccgcacaggc
    10681 atcaagcact cgctcatcac cttgtaaatc aagcgtcgga gcaaccagct gactggactc
    10741 gtcttggatg gtaatggctc catccgcaaa caaattatgc cctgcaaaat gcccttgctc
    10801 cttaaccaga ccagtggttg ctaaaaggga atcattcgcc tccaacaagg cttggatttc
    10861 ctcttttcga cttaggtctg ttacacgaat actggctttg tttcgcacta acaggctttc
    10921 aaagatggct tttgctctct cctctccgta ttcttccttg agtttggtaa ctagccaaac
    10981 tgggagagaa taggcaatgg agtcacgctt gttttttcgc ttgatgctag caatatctgg
    11041 ccagccttca cgcaagatac ggcgaaggac agcgttgacc aatttttcac tgcctttttt
    11101 acggagtttg gccaattcca ctgcttcatt gaccacagca tgatctggaa tcttgtccaa
    11161 atagcggagt tggtaggcac tcatgagaag aaggacatag agccagctgt ctaactggtc
    11221 tctgtcttcg ataaagtggg ataagtacca ttccagagtc agtttacggg ctaccgttcc
    11281 atagactagc tcggtcacta agcccttgtc tgctgcagag agctgactcc ccttgaggtg
    11341 tttatttaag gcaatatttg aatatgcttg attcacaaaa acatcctcta gcactgctag
    11401 agctaaactt ctagccgttt ctactttagt caccaaatcg ttctcctaca gtcaatgtac
    11461 gtccaactcc gttgaggaag gaagcaatgt ccatcttagg cttaccagct ggctgcactt
    11521 gtttgaggga tagagcccct tcagccgttg cgacaatcaa ttctttcttg ccgatagaga
    11581 gaatctcacc tggatttccc tgatcttcta ctggtagggc ttcataaatc ttaaagcggt
    11641 cgcccttaag gaaagtatgg gcaacaggcc aggggttcat tccacgaatt tggttaaaga
    11701 gttgacgatt ggttttgttc cagtccagtt tttcttcctc tggctttata tttggagaga
    11761 aggtaacctg actcgtatcc tgcggttcag gtttgatatc accagcaata taggcaggca
    11821 gagtgtccaa aagcaaatca cgaccaacta gcgccaattt ttcaaacaag gtgccaacat
    11881 tgtcctcatc tgtgatcgga atgctgcgac gaaaaatcat atctcctgca tccatttcct
    11941 taaccatttc catgatggtc acaccagctt cctcatcccc ttgaatcaag gcataatgga
    12001 taggcgcacc accacggtgt ctaggaagga gggaggcatg aacgttgaca gcaaagtcca
    12061 tgctatcaag gagtttgctt gggagaaact gcccaaaagc agcagtcaca attccatctg
    12121 ctcctagctt cataagttct tccatctctg gacttccaga taatttttca ggttggtaga
    12181 tagatagtcc tgcttccttg gcagcctgct tgactggggt ttcttggata acttttttac
    12241 gaccaacagc acggtctggc tgggtcacaa cggctagaat ttcgtaacgg tcatctgtca
    12301 aaagtccttt taagactgtt gctgaaaagt cgggggtccc cataaagatt agttttgtca
    12361 tatcttctcc ttcttataaa aattgctgcg gctcatggtc aatgctgaga cggagctcac
    12421 tattttcccg ttcttgagtc aaggctaaaa cctggttgag ggtcggcccc agctcatctt
    12481 ctaaacggta tttaattaaa atctggtaat gatagaggtt gtgggtacgg gcaatcggtt
    12541 ttggcgttgg ccccagaatg ggactggtct ctgacaagcc tgaccgcaaa atgttcatga
    12601 cttcataggc acgtttgaaa acctcttctt ctttcttgtg agaaagggta ataccaatcg
    12661 tgaaatagta aggtggatag ccgagttgtc gtctgattcc catttcatag gcataaaagc
    12721 cttcgtaatc ttgatccttg gcaaatcgaa tagcatagtg ctgcggattg taggactgta
    12781 tcaagacttg acctgccttt tcagcccgac ctgatcgacc tgccacctga gtcaagagct
    12841 ggaaggttct ctcagaagaa cggaaatcag gcagattcaa ggccgtatcc gcatttagaa
    12901 ctccgactag ggtaacattg ggaaaatcca aaccctttgc aatcatctga gtaccaagta
    12961 aaatatccgc ttcccctcgc ccaaactggt caagcaaggc ttggtgactg cctttctttc
    13021 gagtcgtatc cacatccatc ctcagaatgc gagcttgggg aaagagttct gctagctcat
    13081 cataagcctt ctgagttccc gttccatagt aacgaatact gtggctctta cagttaggac
    13141 agacctgagg aatatccttc gagaaaccac aataatggca gttcatagtc ttggtatcca
    13201 tatgcaaggt cagagaaata tcgcagttgg gacaagtatc caccgtccca cactcccgac
    13261 acatgacaaa gctagaataa ccacggcgat tgagcatgag aaccacctgc tcttttttaa
    13321 ccagacggtc ttggatagcc tctagcaaag gaggcgtaaa gtttgacgtc tcattttgtc
    13381 cgatatagtc tcgaaagtca atcacttgaa cctcagggat tgtagccaaa ggattggcac
    13441 gttgggttag acgtaagtgt tgatagacgc ctttgccagc acgtgcccgg ctctctaagc
    13501 tcggcgttgc agatccaagt accagagttg cttgattata ctgagcccgt aaaatagcta
    13561 cctctctggc atggtaacgg ggattgctgt cctgcttata agccgcttca tgctcttcat
    13621 caataatcat gacacccaga tttttcagag gagcaaagat agcagatctg gcaccaacaa
    13681 caacttgggc atcgccacgc tccaccttgc gccattcatc atacttttcg ccattggata
    13741 atcctgagtg aagaatggct accttgtccc caaaacgtgc tataaaacgc tcggtcatct
    13801 gaggagtcaa ggaaatctca ggtaccagca aaatagctgt cttgccctta tccagggcac
    13861 cttggataat ctgcaagtaa acctcggtct tcccacttcc tgtaatccct tgaagtagaa
    13921 agggaggttg agaactgcca atagaactca caaccgcatc acgcgcctgt ctttgttctg
    13981 gatttaactc caaaggtcta cttgcttcaa ttccttcaaa ataagcagcc gagcgttgaa
    14041 cttccttttg gactatggta acagcacctt gatccacaaa gaagttgact tgctctcgcg
    14101 agtaggactc taacaagcta gccaaggaag cgctctctgg atgagacagc agataatctc
    14161 tcagttccaa ctttttcttg gcacgtgtag aaatctcaac accttctaat tgggcatggt
    14221 caacctcata ccaagactgg gtcttgacct tcttttgatc gactgcctga tattccagac
    14281 caagcaggcc ttttctagtc aaacgcatca tttcagcttg cttacctaaa tccagagaag
    14341 aaaaggctag cgaatcttct gaatcaaaca ggcgtactcg ttcttcctga ctcaagcctt
    14401 ccagaggata gagaatcttg tcatagctag aattcagaaa tcccggaagc atggccttga
    14461 ggatagagat tttgtaggag aagacagatt tgcgtaactc ctcagccagc cagagttgtt
    14521 ctggcgtgag aacaggagaa aaatccagca cctctgcaat atcttttaaa tcttgctcca
    14581 tctcttctcc atctgattgg gactccaaac caagaacaat cccttgaatc aggcgattac
    14641 ccttaccaaa aggcacatga acccgcatcc caacttccag cattccctca aattcctccg
    14701 gaatcctgta actatagggc tggtccgtct gcatcaaggg cacatctacg ataatcttag
    14761 ctagggccat cttctcacct cctccttgtc agtacattct tgcaatagaa aaaataagat
    14821 tgagtccccc caaccttaaa ttttttcacc atcttctttt tctttagcaa tttgctcttt
    14881 gattttcttt tcttcttctt ctttgcggcg tttttcttct tcgatacggc gacgcactgc
    14941 ttcacgtttt ccttctggat ctgggtgaat tgtaacgttt cctgattcga tttcttctaa
    15001 agcgcgaaga gttgattttt cagacttgaa accttgagtt gctggggcac ctgcttccaa
    15061 ttcgtgggca cgttttgctt ccaagattac gagtgaatat tttgaaggaa ccttgtcgag
    15121 caaggtatca atagagggtt ttaacatcat ttgcttgtac ctattttcta aattttatcg
    15181 ggtagttgga gattttggta acatctcctg atagtgacca atgacacgat ccacacagaa
    15241 gtgttctgct tcaatcacac atttgacacg ttcagcagct aggggcacct gatcgttgac
    15301 aatcgcataa tcatactcac gcatgagggc aatttcttcc ttggcctttt cgattcgttg
    15361 ggcaatcact tctgcactat ctgttccacg acctaccaag cgatcttgca attcatccaa
    15421 atctggtggt gtcaggaaga taaagacagc atctggaacc tttttcttga cctgaagagc
    15481 accctgaact tcaatttcaa ggaaaacatc gattcccttg tccaaggttt cattgacata
    15541 ggtcagagga gttccatagt agttgccgac atattctgcg tattccaaca tttgtccttg
    15601 acgaatcagc tcttcaaatt cttcacgagt acggaagaaa tagtcaacac cgtccacttc
    15661 tccaggacgt tgtgcgcgtg tcgtcatcga tacagaatat tgaaattggt tttcagaact
    15721 ctcaaaaatc tctcttctaa ccgttccttt tccaacccct gaaggaccag aaaaaacgat
    15781 tagtaagcct cggtctgcca ttgtgtctcc ttttagtcaa tctgtgaaat aacatttctc
    15841 tagaataatg gcaaaaagcc agattatcct ttacagtctt tctatctagt gtaacaaaaa
    15901 agcagtaatt tttcaactgc tctttcttat ttatttagca taatctactg cacgaagctc
    15961 gcgaatcacg gttaccttga tatttcctgg ataatcgaga ttgttttcaa ttttcttacg
    16021 aactttgtga gccaagattg tgactttgtc gtccttgatt tttcctggat tgaccatgat
    16081 acgaatttca cgtcctgctt gaagggcaaa gctagtttgc actccttcaa agccgttagc
    16141 aatttcttcc aaatcatgga gacgcttgat gtagctttca agagactcac tacgagcacc
    16201 tggacgggct gcgctcaagg catctgctgc agcgacgata actgctatca cgctctcagc
    16261 ttcaacatct ccgtggtgac tagcaatcgt attcaccaca actgggggtt ccttgtactt
    16321 acgggccaat tccataccga tttcaacgtg gctaccttca acctcatggt caatggcttt
    16381 cccgatatcg tgaaggaatc cagcacgacg ggcaagagcc gcattttcac caagttcgct
    16441 cgccatgata ccagccaact tagccacctc aatcgaatgg cgcaaaacat tttgtccata
    16501 tgaagtacgg aactgcaaac gtcccataat cttcatcaag tctggatgaa ggtttggcgc
    16561 accaatttca taggcagcag cctcaccgta ttcacgaatc ttattgtcaa tctcttgacg
    16621 gtttttctca accaactctt cgatacgagc tggatgtata cgaccatctt tgagcaacat
    16681 ttccatagtc atacgggcaa tctcacgacg aatcggatca aatcctgaca aggtcaccac
    16741 ttctggtgta tcgtcgataa tcacatcgac ccctgtcaaa ctttcaaagg tacgaatgtt
    16801 acgaccttca cgaccaataa tgcgtccctt catagtatcg tctggcagat gaactgttga
    16861 gtttgttgac tccgctacat attcaccagc gatacgttgc atagcttgaa ccaagatgtc
    16921 cttggccatt ttgtcagaac gttccttgac ctcttgctca gcttcgcgaa tgcgactggc
    16981 aatctccctg gtcaagtttt cctctgtctg aaccaagata atatctcgtg cttctgcctg
    17041 agacagagca ccaatacgct ctagttctgc ttctttttgt ctttcgactt cctctaattg
    17101 ctcttcacgc gcatcaaggt ttttcgctct atcagaaata ctttgttctt tttgttcaag
    17161 tgtttgttct ttactcgtca aattgtcgtc cttacggtca aggctagtag ctctctctgt
    17221 caaacgactt tcgatttgtt tgagttcttg acgttctgat ttgaattcag cgtccacttc
    17281 ttcacggtat tttctggctt cttctttggc ctccaatagt gcttcttttt taagagactt
    17341 gctttcacgt ttggcttcat taacaagtaa atccgcttca cgctcagctt gtccacgtaa
    17401 attagttgct tcttgttcag catttaaaag catcaactct gcagcttcct gagatgattt
    17461 catcttagct gagatgctga catatccaat gactaaacca atgatgacgg caaaaacagc
    17521 aatcgcaagc gacatgattt ccatgttttt acctcatttt attgttattc cgaatgacat
    17581 acattctttt acattctacc ataaaaaagt gattttcaca aacctaaaat agaatatgtt
    17641 ttggggaaat tagtctgaat tttaggatta cacttccttg gttttttaag gagataagtc
    17701 agtgccgcta tcaaaaagtt ccaccgaatt tctactgcta tggattttct attgttgtgc
    17761 caaatagtgt tccactagat tcaaacattt tgctttcaag tctttgaaaa ttcacttt
//
LOCUS       DAVMAF010000036        16666 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMAF010000036 DAVMAF010000000
VERSION     DAVMAF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.036"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..245
                     /locus_tag="VYO97_001250"
     CDS             <1..245
                     /locus_tag="VYO97_001250"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754042.1"
                     /translation="EVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVKKDAETLKQE
                     ANGNKTVSMNNTVKLKEAVYKKLLQQTDSFKTSIFK"
     gene            306..2027
                     /gene="pabB"
                     /locus_tag="VYO97_001251"
     CDS             306..2027
                     /gene="pabB"
                     /locus_tag="VYO97_001251"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1754043.1"
                     /translation="MHRKTVIDFRTLGERYTFTKPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSSIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            2147..3673
                     /locus_tag="VYO97_001252"
     CDS             2147..3673
                     /locus_tag="VYO97_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1754044.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            3760..4679
                     /locus_tag="VYO97_001253"
                     /pseudo
     CDS             3760..4679
                     /locus_tag="VYO97_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            4814..5773
                     /locus_tag="VYO97_001254"
     CDS             4814..5773
                     /locus_tag="VYO97_001254"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1754045.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            5870..6709
                     /locus_tag="VYO97_001255"
     CDS             5870..6709
                     /locus_tag="VYO97_001255"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1754046.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(6757..6927)
                     /locus_tag="VYO97_001256"
     CDS             complement(6757..6927)
                     /locus_tag="VYO97_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1754047.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            7048..7932
                     /gene="miaA"
                     /locus_tag="VYO97_001257"
     CDS             7048..7932
                     /gene="miaA"
                     /locus_tag="VYO97_001257"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1754048.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            7925..9163
                     /gene="hflX"
                     /locus_tag="VYO97_001258"
     CDS             7925..9163
                     /gene="hflX"
                     /locus_tag="VYO97_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1754049.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQFKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            9156..9779
                     /locus_tag="VYO97_001259"
     CDS             9156..9779
                     /locus_tag="VYO97_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1754050.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            9794..10723
                     /locus_tag="VYO97_001260"
     CDS             9794..10723
                     /locus_tag="VYO97_001260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1754051.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            10744..11499
                     /locus_tag="VYO97_001261"
     CDS             10744..11499
                     /locus_tag="VYO97_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1754052.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(11553..12461)
                     /locus_tag="VYO97_001262"
     CDS             complement(11553..12461)
                     /locus_tag="VYO97_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1754053.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            12525..12794
                     /locus_tag="VYO97_001263"
     CDS             12525..12794
                     /locus_tag="VYO97_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1754054.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            12794..13174
                     /locus_tag="VYO97_001264"
     CDS             12794..13174
                     /locus_tag="VYO97_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1754055.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            13263..13433
                     /locus_tag="VYO97_001265"
     CDS             13263..13433
                     /locus_tag="VYO97_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754056.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNI"
     gene            complement(13530..14255)
                     /locus_tag="VYO97_001266"
     CDS             complement(13530..14255)
                     /locus_tag="VYO97_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1754057.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            14417..16258
                     /gene="typA"
                     /locus_tag="VYO97_001267"
     CDS             14417..16258
                     /gene="typA"
                     /locus_tag="VYO97_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1754058.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            16278..16532
                     /locus_tag="VYO97_001268"
     CDS             16278..16532
                     /locus_tag="VYO97_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1754059.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         5055 a         3089 c         3727 g         4795 t
ORIGIN      
        1 aagaagttaa agataaagca acgaaaggat taacaacatt cgaagtaaat ggttcttccg
       61 tttcatcata cgatgattta ctgacattgt ttaaagaagc tgttaaaaaa gatgccgaaa
      121 ctcttaaaca agaagcaaac ggtaataaaa cagtatctat gaataataca gttaaattaa
      181 aagaagctgt ttataagaaa cttcttcaac aaacagatag ctttaaaact tcaatcttta
      241 aataattgaa atctctcgtc tgctttgcgg atgagggttt tcttatttta tgctatactt
      301 aaggtatgca tagaaaaaca gtgattgatt ttaggacttt gggggagaga tataccttta
      361 ccaagcctat taaagagttg aaaacgagaa atgtagaaga agtggcagat ttgctggcac
      421 aagtggaaag ctaccaagag caaggttatt atgtggtggg gtatgtcagc tacgaggctg
      481 cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc cctactggga gagtacctgc
      541 tttattttac tgttcatgat agcgtagaaa cttcctctat tcctctgact tatgaggata
      601 ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc agactatgaa aaggccattg
      661 cccaaattca ccatcattta cggcagggag atacctatca ggtcaactac accgtccaac
      721 ttaagcaaaa gttaaatgcc aatccttttg ccatctacaa tcgtatggtg gtagagcagg
      781 aggcgggcta caatgcctat gtggaacatg acgagatggc agtgatttcc atgagcccag
      841 agctcttttt tgagcaaaat gatcgcgagt tgacaacacg accaatgaag gggacgactc
      901 agcgtggggt aactgaccaa gaagatcttg aacaggccag ttggttggaa caggatccca
      961 aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg caatgatatg aaccgtattt
     1021 ctgaagttgg gagcgagcac gtggagcgtc tgtgtcaggt agagcagtat tcaactgttt
     1081 ggcagatgac ttcgaccatc aagagtcagt tgcgagagga tgtggacctt gttgaaatct
     1141 tccgctcact ctttccttgt ggttccataa cgggtgcacc gaaaattgcg acaatggaga
     1201 ttatcaagga cttggagcct caaccgcgtg gagtctactg tggaacgatt ggtctcttgc
     1261 ttccaaatgg acgacggatt tttaatgtgg ccattcgtac cattcaactt cacaaaggtc
     1321 aagccattta tggagttggc ggagggatta cttgggatag cacatgggaa tctgaatacc
     1381 gagaggttca tcaaaaggca gcagttcttt atcgtaaaca agctcgcttc caactgatta
     1441 cgacagggga aatcagccag aagaacctgt tgtttgaaga tcaacatctg gaaagactga
     1501 gaaaagctag tcgttatttt gcctttcctt ttgatgcaga agacttggga cacaagattg
     1561 aggaagagtg tcaggattgt gaagctaatc gagattaccg cttgcgaatc agccttagca
     1621 aatctggaga gatagaagtc aatcgtcaag tattaacccc tctcagtaca agcttttgtc
     1681 aggcccaagt ctgccttcag gaagctgctt tgaatcaatc ctttacctac tttaaaacca
     1741 ctcaccgacc gcatttgagc ctaggagaac aagagaagat ttaccacaat aagtcaggag
     1801 aactgcttga aacctctata ggaaatttgg ttctgaaaat cgctggaaaa ctctacacac
     1861 cgcctatccg acttggaatc ttgccaggaa tttaccgtca gtatttgcta gaaacaggac
     1921 aggtagaaga gaaagtcttg accttggcag atttagccca agcagaagct atttacggct
     1981 gtaatgcagt gagaggcttg tatgagttgc aagtgatagg attgtgagtt ttagctatta
     2041 atagttttta atatatatta aaatatattg gtgtttactt ttgatgagat atcatatttg
     2101 tgttataatg taaccgtaaa caatagaaat ttaaaggaga tttttgatga aatcaaaaat
     2161 agttttggga gcttcgctag cgatcgcaac acttagctta gtatctcttg tcgagattga
     2221 aggactctca ccgtttttaa tagagaatgt tagcgcaaat actcattctg caaataaagt
     2281 tattaatcat aaagtaagta tttacttaga aaatgctgat gaaggtaaag gtctaactgt
     2341 aaacttttca acagacagtg ttagtccaaa tttatttgat gagttcgaaa aaaagagtgg
     2401 aattacaata actactatgt tagtgaatgc taagacaggt gaagttgtag aaaaaagatt
     2461 aacaccaagc gtttttttac gttccaatga tttaaccagt gggaccatat cttcgtttat
     2521 ttttagtgaa tatcctgatg gagaatataa atatgttgtg tctaaaggag actttatcga
     2581 tcctaagact cagtttaaac atcagtatag aggagaatct ccagttttta gaatacgtaa
     2641 tcgaaaatat gtggagttgg gaacaactga taaaaaatta gatgaaagac gtgataatag
     2701 cgtttataaa gatggggttg ttgaacacaa ggtaaattta agtttaacaa gttatcaagg
     2761 aggtaatgga gttactgcaa ttttttcaac tgatagtgtt aactctaact tacttaattc
     2821 ttttggtgaa aaggctaaaa aagttttgat taggtctaag ttaattaatg ttaagactgg
     2881 cgaggttatt gatgaaacgt tttctccaaa agtaagttta acctctaaaa ttttgaaaag
     2941 tggttctaca gcagtatttt attttataga tttaactgat ggcgaatata aatatgttgc
     3001 gtatgaaagc cagcaatata ctgatcctca aacaacattg actcatcagt atagaggaga
     3061 atctcctatt tttagtatca aagatggaaa attttctggt ttagtatctg cctcaaaacc
     3121 agacgaaaac ccgaagccaa cgccaaaacc ggatgagaaa ccaaaacctt cagcaccaca
     3181 acaggagaaa acaaaaccta cagttcagtc tggttgggta ggttcatctt attatcaaaa
     3241 tggtaaaaaa gtcactagta agtggatttt tgataaaaaa tataattcat acttctatct
     3301 agatgcatca ggaaattatg ttcaaaacgc ttgggtaggt aactattatc tcaaatcagg
     3361 cggatacatg gctaaaagtg agtggattta tgataagaac tatggttcat actactatct
     3421 aacatcagaa ggcagctacg ctcgtaacac ttggtcaggc aattattatt tgaagtcaaa
     3481 tggtaagatg gctaaaagtg agtgggtata cgatagtaac tacaaatcat attactactt
     3541 gacatcagaa ggaagttatg ctcgtaacac ttgggtaggc aattactatc ttaaatcaaa
     3601 cggtaaaatg gctgttaatg aacgtacacc agatggttat cgtgtagatg gttcaggtaa
     3661 atgggttaaa taagaaacta aaaaatagga taataggtaa tatcagatga ataaaaaaaa
     3721 ttattttcat gtgctttagc attttcaacg attggaacat tgtcaacgat tgataaatca
     3781 ggagttcttc ctttttcagc gcaaacggtg caggctgaca tacaaacgct taccccaatg
     3841 gcaaaatatt atattattct tccgaaggat gcagaaatat ataaaacttg gagaggtact
     3901 gtcaatattc ccattattga tgctacaaaa actactccag aattatctta tttcaaagaa
     3961 gatcatcgca actatatagc taatgagaat aagtctggtg ccaattacat agagtggaag
     4021 ggaacagtag aagagtttaa agaggccatt aagaagttga ctgataaaaa atcaactact
     4081 gcaacaccaa aaaaggatga aaagccaaca ccaaaaccgg atgagaagcc aaaaccaaca
     4141 cctacagttc agtctggttg ggtaggttca tcctattatc aagatggtaa aaaagtcatt
     4201 agtaagtgga tttttgataa aaaatataat tcatacttct atctagatgc atcaggaaat
     4261 tatgttcaaa acgcttgggt aggtaactat tatcttaaat caggcggata catggctaaa
     4321 ggtgaatggg tttatgatgc cacctatcaa gcttggtatt atttgacatc agatggttct
     4381 tatgcttaca gtacatggca aggaaattac tatctaaaat cggatggtaa aatggctgtc
     4441 aatgaatggg ttgatggtgg acgttattat gttggcgctg acggagtttg gaaggaaggt
     4501 caagcaagta cagcttcttc tagtaatgat agcaatagtg aatattctgc tgctttagga
     4561 aaggcaaaaa gttataattc gttattccac atgtcaaaaa aaaacgtatg tatagacaat
     4621 taacttctga ttttgataaa ttttcaaatg atgcagctca atatgccatt gatcattaga
     4681 tgattaacag atactggtag gcgaaaaaat tccttataat tcatttacgc aaacctttgc
     4741 ataagtgaat ggattttgat atactagtac tgtaagcatt ttcaattact acacaatgaa
     4801 aaaggagaat atgatgagtc aaaagattat tgggattgac cttggtggaa cttctatcaa
     4861 atttgcaatc ttaacaacag caggagaaat ccaaggaaaa tggtcaatca agaccaacat
     4921 tttggatgag ggaagtcata tcgttgatga tatgattgag tctattcaac atcgtttgga
     4981 cttgcttgga ttggcagcag cggacttcca aggcattgga atgggatcac caggtgtggt
     5041 tgaccgtgac aaagggactg ttatcggtgc ctacaacttg aactggaaaa cccttcaacc
     5101 aatcaaacaa aagattgaaa aagctttggg cattccattt ttcatcgata atgatgccaa
     5161 cgtagcagct cttggtgagc gctggatggg tgctggagat aaccaaccag acgttgtctt
     5221 tatgacactc ggtactggtg ttggtggcgg tatcgtcgca gaaggcaaat tgcttcacgg
     5281 tgttgctggt gcagcaggtg agcttggtca catcactgtt gactttgacc agccaatctc
     5341 atgtacttgc ggtaagaaag gctgccttga gacagttgct tcagcaacag ggattgtcaa
     5401 cttgactcgt cgctatgccg atgaatacga aggcgatgca gccttgaaac gcttgattga
     5461 taatggagaa gaagtaactg ctaagactgt ctttgatctc gcaaaagaag gagacgacct
     5521 tgctttgatt gtttaccgta acttctcacg ttacttggga atcgcttgtg ctaacattgg
     5581 ttcaatccta aacccatcaa caatcgtcat cggtggtggt gtgtcagctg cgggagaatt
     5641 ccttctacaa ggtgttcaaa aagtttacga tgaaaatagt ttcccacaag tacgcacatc
     5701 tactaaatta gctcttgcaa ctctaggaaa tgacgctgga gttatcggag cagcatcact
     5761 tgtattgcaa taagataatg agagaggaaa atgctaacct agagttagta atgttcctct
     5821 tttatttttt atgctatact agttaggaca atgaatgagg tgagagtaaa tgacaaaagc
     5881 agatacgatt tttaaagaga atattgaacg cattctaaag gaaggtgttt tctcagagca
     5941 ggctcgtcct aagtacaagg atgggactgt tgctaattcc aagtatgtaa ctggtgcctt
     6001 ttccgagtac gacttatcta aaggagaatt tcctatcaca accttgcgtc ccattgcaat
     6061 caaatccgcc atcaaagaag ttctctggat ttaccaagac cagtcaaata gcttagaagt
     6121 gcttaatgac aagtacaatg ttcactactg gaatgactgg gaagttggag acacgggaac
     6181 cattggtgag cgctatggtg ccgttgttaa gaaacacgac attatcaata agcttctcaa
     6241 acagttggaa accaatcctt ggaaccgccg caatattatt tcgctctggg attaccaagc
     6301 tttcgaagaa acagatgggc tgctcccgtg cgcctttcag accatgtttg atgttcggcg
     6361 tgttgatggg gaaatctatc tggatgcgac cttgacccag cgctccaatg atatgctggt
     6421 ggcccaccac atcaacgcta tgcagtatgt ggctttgcag atgatgattg ctaaacattt
     6481 tggctggaag gttgggaagt tcttctactt catcaacaac ctccatatct atgataatca
     6541 atttgaacaa gctcaggaat tgctccgtcg ggagccgtca aactgccaac cacgcttggt
     6601 tttaaatgtt cctgatggga ctaatttctt tgatatcaaa gcagaagatt ttgagttggt
     6661 ggattatgac cctgttaagc cacagttgaa gtttgaccta gctatttaaa agaatagaaa
     6721 aaagaagttg agaataatcc caacttcttt tgtttcttaa cgtgatacgc ggcgacgagc
     6781 tgctttttta cggttttctt cgatgaaagc tgctttttgc tcttctggtt cgattacttt
     6841 ctttttaaat gcgtatactg cacctgcaac ggcagcgaca gttcctgcga cacctgttac
     6901 aagaccttta gcgaatcctt tagccatgag tcttcctcct ttatattctc aatcagccag
     6961 cctcctcaag aggtcacatt tttctgactg acctttttgt gttataataa tagtaacgaa
     7021 aaaatgggaa tttttcaagg aaaaaagatg aaaacaaaaa taattgtgat tgttggaccg
     7081 actgctgttg gaaagacggc tctagctatt gaggttgcaa agcgctttaa tggcgaagtg
     7141 gttagtggag atagccagca agtctatcga ggacttgata ttgggacggc caaggctagt
     7201 ccagaagagc aggcagctgt tcctcatcat ttaatcgatg ttagagagat aaccgagtct
     7261 tactcggctt ttgattttgt ttcagaagct aagatgacta ttgaggatat tcagagccgt
     7321 ggcaagctag ccattatcgc cggtgggact ggactttata tccagagctt gctagaaggt
     7381 taccacctag gtggggagac tcctcatgag gagattttag cttatcgagc tagtttggag
     7441 ccatattcag atgaggaatt agcccatttg gtggagcaag caggccttga gattccccag
     7501 tttaatcgtc gtcgtgctat gcgtgcctta gaaattgccc attttggtca ggatttggaa
     7561 aatcaagaga tcttgtatga accgctgatt atctgcttgg atgatgaacg tagtcaactt
     7621 tatgagcgta tcaaccaccg agtggatttg atgtttgagg ctgggctttt ggatgaggcc
     7681 aagtggctgt ttgaccattc ccctaatgta caggctgcta aaggaattgg ctataaggaa
     7741 ctctttcctt atttccgtgg agagcagacc tttgaggaag ctcgtgagag tcttaaacag
     7801 gcgacccgtc gttttgccaa gcgtcagctg acctggttcc gtaatcgcat gcaggtcacc
     7861 ttttatcaga ttggagaatc tggtgtacaa gaccgcattt taagccagat agaggagttt
     7921 ttagatgatt gaaacggaga aaaaagagga gcgagtcctg ctgattggtg tggaattgca
     7981 gggtatggac agttttgacc tctccatgga agaattggct agtttagcga aaacggcagg
     8041 ggcagtcgtt gtagatagct acagacaaaa acgtgaaaaa tatgattcca agaccttcgt
     8101 cggctctggt aagttggaag agattgcgct tatggtggat gcagaagaaa tcactactgt
     8161 catcgtcaac aatcgtctga ccccaaggca gaatgtcaat ctagaggaag ttctcggtgt
     8221 taaggtcatt gaccgtatgc agttgatttt ggatatcttt gccatgcggg ctcgaagcca
     8281 tgaagggaag ctccaagtcc acctagccca attcaaatac ctcttgcctc gcttggttgg
     8341 tcaggggatt atgctcagcc gtcaggcagg gggaattggt tcccgtggtc ctggtgaaag
     8401 ccaactggag ctgaaccgtc gtagcgttcg caatcaaatc acggatatcg agcgccagct
     8461 taaggtggtt gagaaaaatc gtgcgactgt cagagaaaaa cgtttggagt ctagcacttt
     8521 taagattggt ttgattggtt atactaatgc tgggaaatca actatcatga acatcttgac
     8581 cagtaagacc cagtatgaag cagatgagct ctttgcgact ctggatgcga caaccaagag
     8641 tattcatctg ggaggcaatc tccaagtaac tttgacagat accgttggct ttatccaaga
     8701 tttgccgaca gagttggtgt ccagtttcaa gtcaaccttg gaagaaagca agcatgtgga
     8761 ccttctggtt catgttatcg atgctagcaa tccttaccac gaggagcatg aaaaaacggt
     8821 tctctccatc atgaaagacc tggacatgga agatattcct cacttgacgc tttataataa
     8881 agcggatttg gtggaggatt tcacgcctac ccaaacgcca tataccctca tttctgccaa
     8941 gtctgaggac agtcgtgaaa acttgcaagc attattgcta gataagatta aggaaatttt
     9001 tgaagcattt accctgcgag tgcctttttc aaagtcctac aagattcatg atttagagag
     9061 tgttgcaatt ctggaagaac gtgattatca ggaagacggc gaagtgatta caggctacat
     9121 ttccgagaaa aataaatgga ggttagaaga attttatgac tgatattaaa actttggctc
     9181 taaagtatgg aggttataca agtctggaca aggtctatct ggatcagctt ctagctggta
     9241 aaacagatca ggagcagtta acacttatta cacctccgcc aagtgttgtt aatgcctact
     9301 ttgcagaact ctatcagaaa aagagtcctc aggctgcgac ggattatttt gcagaactca
     9361 gtcaggaact gaatctttac aatactgagc caagtttcac cctagaaaat aagcctttta
     9421 ttcggcttaa tctgtctggt aaatcctttg gtttttgtta tgagagtgac ggccttggtc
     9481 ggattttctt tgaaaatgag gagaaaatct cggatgactt gctctttgaa attgcgcaaa
     9541 ttttccccca tcaattagtc tttgaagagt ctggtaagat ttacatgaag cctgtcgagg
     9601 acgaggaagt tatcagccta gaaagtctca cagctttgac tgatttggaa agattggctg
     9661 atggtcgaaa acgtctcaaa ggctacagcc aagaggattt attgcaagaa gctgctgctt
     9721 tttctggcaa acgctatttc cgatcggaaa accgcacagc catgttatat attgattaat
     9781 tagaaagtat cgaatggata ttcaattttt aggaacgggg gctggtcagc cctctaaagc
     9841 ccgcaacgtt tcaagtctcg ccctgaaact tttggatgag attaacgaag tttggctctt
     9901 tgactgtgga gaaggtacgc aaaatcgcat tctggaaacc acaattcgac cacgtaaggt
     9961 cagcaaaatc tttattaccc atctgcatgg agaccacatt tttggtttgc caggtttcct
    10021 ttctagccgt gcctttcagg ccaatgaaga gcagacagat ttggaaatct acggacctca
    10081 aggaatcaag tcatttgtct taaccagcct tcgtgtgtca ggttctcgtc tgccctaccg
    10141 cattcatttc catgagtttg accaagattc tctaggtaaa attcttgaaa ccgataaatt
    10201 cactgtgtat gcagaggagc tggaccacac tattttctgt gttggctatc gtgtcatgca
    10261 aaaggatcta gaagggacgc tggatgctga aaaactcaag gctgctggtg ttccgttcgg
    10321 cccgcttttt ggtaaaatca aaaatggcca ggatcttgtt ttggaagacg gaactgaaat
    10381 caaggcagca gactatatct cagcgccacg tccaggtaag attatcacta ttttaggaga
    10441 cactcgaaaa acggatgcca gtgtgcgtct ggctgtcaat gcagatgtcc tagttcatga
    10501 gtccacttat ggcaagggtg atgaaaaaat tgctcgtaac catggtcact caactaatat
    10561 gcaagctgca caagtagcgg tagaagcagg tgccaaacgc ctcctactca accatatcag
    10621 tgcccgtttc ctctcaaaag atattagcaa actcaagaaa gacgctgcca caatttttga
    10681 aaatgtccat gtggtcaaag acttggaaga agtggaaatc tagcagtcac agaaaggata
    10741 agtatgccta ctattctcat taccggagct agcggtggtc tagctcaaga aatggtcaaa
    10801 ctcctgccca atgaccaact catcttgctt ggtagaaata aggaaaaatt agcccaactc
    10861 tacggaaatt attcccatgc agaattgatt gaaattgata ttaccgacga ttcagcccta
    10921 gaagctctgg taactgatct ttatctccgc tatggcaaga ttgatgtctt gattaacaac
    10981 gctggttacg ggatttttga gggatttgac cagattgctg ataaagatat tcaccagatg
    11041 tttgaggtca atacctttgc cctgatgaat ctgtctcgtc accttgcggc tcgtatgaag
    11101 gaaagcagca aagggcatat catcaacatc gtcagcatgg caggtctaat agctactggc
    11161 aagtctagtc tttactcagc gaccaagttt gcggctattg gtttttcaaa tgctctgcga
    11221 ctcgaactta tgccctatgg agtctatgtg acaacagtca atccaggtcc aatccgaaca
    11281 ggattttttg accaagctga cccagatgga acttatctta aatcggttga ccgcttcctc
    11341 ttagaggcag atgcagtggc taaaaagatt gtcaagatta taggcaaaaa taaacgagaa
    11401 ctcaatctcc cgattttgtt gaacctagcc cataagtttt atactctctt tcccaagcta
    11461 gctgataagt tggcagggga aacttttaat tataagtaaa aagaaccaat gtgcaggttg
    11521 ttgctagcct acatattggt tcttatcatt tctcaactat caaactgaac ttcttctagg
    11581 agatagtcga taaagcgttc gcccatctta gataggctgg ttttctcatg ctggatatag
    11641 accagctcaa tcgggtcatc aatatccagt gggatagaaa cgatattgtc tccgtttagg
    11701 ttgctgttca aaatccctgt cgcaatggta taaccatcca aaccaatcaa gagattaaag
    11761 agggtggcac ggtcactgac cacaatggat ttcttgtggt gttcttgaga aagaatctct
    11821 tctgaaaagt agaaggagtt gtgcgtccct tggtcatagc tgaggtaagg gaaattctcc
    11881 aaatcagaca gtttcacctt gtctttcttt gccagagggt tggtcttgct gacaaagata
    11941 tgcggttgcg ctgtgaagag atggtgggat agcaggtgat tgtcatccag catcttggtt
    12001 aaaacatcac ggttgtagct gttcaaaaat aggacaccga cctcactacg gaaattcttg
    12061 acgtcgtcaa taatctccca agtccgagtt tcacgaagga agagttcgta tttctccata
    12121 tcgcttttct tgagcaaaga gacaaaggca ttgaccacaa aggcatagtg ttgagacgaa
    12181 acgctaaaga gttcgcggtg ggcgacagga tttttatagc gttcctccag aagctgggtc
    12241 tgctcgacaa cctgacgggc ataggagaga aactccatcc catcacgggt caaggtgatt
    12301 cccttgggat tgcggataaa gatctcaatg cccatttcat tttccaaatc tcgcactgca
    12361 ttggagagac ttggctgagt gataaagagt tgcttggctg cctcattcat ggagccagtt
    12421 tcgacgattt tgataatata atgtaattgt tgaattctca tgtttttatt gtaccatact
    12481 tgccttagaa tcgctaaaga agataggaaa ataaggagca ttgtgtgagt ctcttcttga
    12541 aaaaaacaca aaattttgct agaatgagtc ttatgaaaac attctatgat gtgcagcaat
    12601 tcctcaaacg atttggtatt attgtttaca tgggaaaacg cttatatgat attgaactga
    12661 tgaagttgga actatctcgg atttacgatg cagggttgat ggataaatta gactatctag
    12721 aggcagaagc agttcttcgc agagagcaca aggtagaatt ggattatatt gagaaaaatg
    12781 gagaaaagaa ctaatggtta cttggatttt gtgggcactt atactagcaa tgttggcgtg
    12841 gatgggcttt aactatcttc gtattcgccg tgcggctaaa attgtggaca atgaggagtt
    12901 tgaagccttg attcgtacgg gtcaattgat tgatttgcgc gacccagcag aattccacag
    12961 aaaacatatc cttggtgcac gcaatattcc ttcaagtcag ttgaaaacta gtcttgcagc
    13021 ccttcgtaaa gataaacctg tccttctcta cgaaaaccaa cgtgcgcaac gagttacaaa
    13081 tgcagctctt tacttgaaaa aacaaggttt ttctgagatt tatatccttt cttatggctt
    13141 ggattcttgg aaagggaaag tgaagactag ctaaccttat ttccaaatta gtcgaatgat
    13201 aggatgaagc tattgtttta tagtataatt aatttgtata aaatttaagg agttcttaga
    13261 acatgaaaga aaagaagaaa aatccacttt ttgtgggtat cttgtcgatt attcttggtt
    13321 tgttatttcc aattgtaggc ctgattttgg gtatcatagg attggtcttg gctatttcat
    13381 accaaaaaga gtcacaatta gactataaaa tagaaaagat tcttaatatc taaggaattg
    13441 tgatttctgt agttaactgg attgtagcaa tcgccttaat ttttcggtaa ataaaagaaa
    13501 aaagctttag ttttaaagct ttttttgttt tatcgactca tctctaaata gtttttaatg
    13561 atttgtaact ctttttggtt caaagggcgg tagttacctt ctgctaaatc tgggttcaat
    13621 gtgaagtccc caaattggat tcttttgagg ctagtcacct taacaccaac cgagaggaac
    13681 attttcttga tttgatgaaa ttttccttct gaaatggtga tagaggcttg actgagggag
    13741 ggacttgcag atagaatctc tagttttgcg ggtttacaga cagtaccatc taaaaagaca
    13801 attccctttt gaaaggcttg gatatggtca ggtgttagaa gtccattaac ctggacttgg
    13861 taagtcttat cgacatgata ttggggatgg aggagctgaa agcccaaggg accgttatcg
    13921 gtcaagagga ggagtcccgt tgtatctcgg tccagtcggc caacggcata gagcttgtca
    13981 gactggatgt cagatggaag caggtccatg acggtcggaa gttccttgtc tttgttggct
    14041 gtaacgacac cagcaggttt atgaagcata agataggtgt gttcatagcc ttgaatgatt
    14101 cgatcctgaa aaaggagttc ttgtagtcct gtatcgatat tttgagctag ggagcgggct
    14161 gggcaaccat cgactagaat ttcccctctg aggagtgctt gcttcatggc ctttcggctg
    14221 attttttctt gggctaataa attatctaaa cgcatactgt gcctatctta tcataagtcg
    14281 ctcgctttga aaaggcttga cgtgaaaaga aaagcagagt gaaaacattt acacttgctt
    14341 gagttatgtt atttacttga aattatggta taatcgttca gttagaaaat aaattttgaa
    14401 tattatagag gaaatcatga caaaattaag agaagatatc cgtaacattg cgattatcgc
    14461 ccacgttgac cacggtaaaa caaccctggt tgacgaatta ttgaaacaat cagaaacgct
    14521 tgatgcacgt actgaattgg cagagcgtgc tatggactca aacgatatcg aaaaagagcg
    14581 tgggattacc atccttgcta aaaatactgc cgttgcttac aacggaactc gtatcaacat
    14641 tatggacaca ccaggacacg cggactttgg tggagaagtt gagcgtatca tgaaaatggt
    14701 tgacggtgtt gtcttggtcg tagatgccta tgaaggaacc atgccacaaa ctcgtttcgt
    14761 attgaaaaaa gccttggaac aagaccttgt cccaatcgtg gttgttaaca aaatcgataa
    14821 gccatcagct cgtccagcag aagtagtgga tgaagtcttg gaacttttca tcgagcttgg
    14881 tgcagatgac gaccagcttg atttcccagt ggtttatgct tcagcgatca acggaacttc
    14941 ttcattgtca gatgatccag ctgaccaaga agcgactatg gcaccaatct ttgacacgat
    15001 tatcgaccat atcccagctc cagtagataa ctcagatgag cctttgcagt tccaagtgtc
    15061 gcttttggac tacaatgact tcgttggacg tatcggtatc ggtcgtgtct tccgtggtac
    15121 agttaaggtt ggggaccaag ttaccctttc taaacttgac ggtacaacta aaaacttccg
    15181 tgttacaaaa ctcttcggtt tctttggttt ggaacgtcgt gaaatccaag aagccaaagc
    15241 gggtgacttg attgccgttt caggtatgga agacatcttt gtcggtgaaa ccatcactcc
    15301 gacagatgca gtagaagctc ttccaatcct acacatcgat gagccaactc ttcaaatgac
    15361 tttcttggtc aacaactcac catttgctgg taaagaaggt aaatgggtaa cttctcgtaa
    15421 ggtggaagaa cgcttgcagg cagaattgca aacagacgtt tcccttcgtg ttgacccaac
    15481 tgattcacca gataaatgga ctgtttcagg acgtggagaa ttgcacttgt caatccttat
    15541 cgaaacaatg cgtcgtgagg gctatgaact tcaagtatct cgtccagaag ttatcgtaaa
    15601 agaaatcgac ggtgttaaat gtgaaccatt tgaacgtgta caaatcgaca ctccagaaga
    15661 ataccaaggg tctgttatcc aaagcctttc tgaacgtaaa ggtgaaatgt tggatatgat
    15721 ttcaactggt aatggtcaaa ctcgtttggt cttccttgtt ccagcgcgtg gtttgattgg
    15781 atactcaact gagttcttgt caatgactcg tggttacggt atcatgaacc ataccttcga
    15841 ccagtacttg ccattgattc caggggaaat tggtggacgt caccgtggtg cccttgtttc
    15901 tatcgatgct ggtaaggcta caacttactc aatcatgtct atcgaagaac gtggtacgat
    15961 ctttgtcaac ccaggtactg aggtttatga aggaatgatc atcggcgaaa actctcgtga
    16021 aaatgacttg acagttaaca tcacgaaggc aaaacaaatg accaacgttc gttcagctac
    16081 taaggaccaa acagctgtta tcaagacgcc tcgtatcttg acacttgaag agtctcttga
    16141 gttcttgaac gacgatgagt acatggaagt aacgcctgag tctatccgtt tgcgtaaaca
    16201 aatccttaac aaagcagagc gtgagaaagc taacaagaag aaaaaatcag ctgaataaga
    16261 gctagaaaga gagaaagatg gtctatttag tcctaggaat tttactgctc ctactctatg
    16321 tatttgcgac accagaaagc attaaaggga ctgtcaatat cgtcgctatg gtatgtattt
    16381 tagtggcact cttgatttta ttggttctat cttttctgaa aatttttcaa ttaccaacag
    16441 aaatattcct agcaatagcc atgttgatcc tagcttactt tagtgtgaga gacatcacac
    16501 tcatgccagt caaaaaaagt aaaagaagat aaaaaagaga gtttcggctc tctttttgct
    16561 aggaatttta caatagtttg aattgctttt tagttaacac catctctttt acttggatag
    16621 tatgaatggg aaaaacttga ataaaattcg ttaatggcat ctattt
//
LOCUS       DAVMAF010000037        16555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMAF010000037 DAVMAF010000000
VERSION     DAVMAF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.037"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            4..1935
                     /locus_tag="VYO97_001269"
     CDS             4..1935
                     /locus_tag="VYO97_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1754060.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKV
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            1922..3238
                     /locus_tag="VYO97_001270"
     CDS             1922..3238
                     /locus_tag="VYO97_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1754061.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3413..4399
                     /locus_tag="VYO97_001271"
     CDS             3413..4399
                     /locus_tag="VYO97_001271"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1754062.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENIAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4463..4534
                     /locus_tag="VYO97_001272"
     tRNA            4463..4534
                     /locus_tag="VYO97_001272"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4496..4498,aa:Arg,seq:ccg)
     gene            4717..5415
                     /locus_tag="VYO97_001273"
     CDS             4717..5415
                     /locus_tag="VYO97_001273"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1754063.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRETILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5406..8945
                     /gene="smc"
                     /locus_tag="VYO97_001274"
     CDS             5406..8945
                     /gene="smc"
                     /locus_tag="VYO97_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1754064.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKDRTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDEMARLIESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLATIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8942..9736
                     /locus_tag="VYO97_001275"
     CDS             8942..9736
                     /locus_tag="VYO97_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1754065.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9736..10554
                     /locus_tag="VYO97_001276"
     CDS             9736..10554
                     /locus_tag="VYO97_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1754066.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10558..11847
                     /gene="ftsY"
                     /locus_tag="VYO97_001277"
     CDS             10558..11847
                     /gene="ftsY"
                     /locus_tag="VYO97_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1754067.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GIIEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11891..13378)
                     /locus_tag="VYO97_001278"
     CDS             complement(11891..13378)
                     /locus_tag="VYO97_001278"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1754068.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13527..14267)
                     /locus_tag="VYO97_001279"
     CDS             complement(13527..14267)
                     /locus_tag="VYO97_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1754069.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14267..16432)
                     /locus_tag="VYO97_001280"
     CDS             complement(14267..16432)
                     /locus_tag="VYO97_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1754070.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQSQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5181 a         2950 c         3656 g         4768 t
ORIGIN      
        1 ttgatgaata aaagaaaagt tagtttagaa gatttttata aatggtatag tctaaataaa
       61 gaagagttat taaataaggc aactgttggt gaaaagttta atgataaatt aaaagaagag
      121 tttctccagg aatggccttt ggataggatt ttaacaatgt caatcgatga atatgtaata
      181 ggaaagggac agcaaaataa gtctttatgc tacgctcttg agaagggaaa atacaaaaat
      241 ctatttcttg gaatttctgg tggctcagct tcaaaatttg gtatttattg gaataaaaaa
      301 acaaacaaat ataaagatca agctaataat gagatttcag agttggatca gcgattttca
      361 aaattaaaat cagatttgta tgaaattatc aaagaaggta ttcgttttaa ctttgaaaat
      421 cctatttttg atatgaaaag atcaacaaat gaatttattg gtcgttctgc tatggtgaca
      481 aaattacttt gtatctattc tgagggagat cctttctttg gtgtaaatat taatagtcag
      541 aaagaatttt ggaaccactt tgtttctcag acaaatcaag gtggacctta tctgcaaaat
      601 cataaaataa ttgaactggt gtccaaaact tatcctgagt tggagccatc gaaagtagga
      661 actatgcttt ttgagtattc taagcttttt atggaaaata aggaagacaa tagtacaatg
      721 gattcatcaa acaattttcg tcatcaatta actcaatctc tattaaagtc tccaaacctt
      781 atccttcgtg gtgctcctgg cacaggaaag acttatcttg ctaaagaaat tgccaaagaa
      841 ttaacggatg gcaacgaaga ccaaatcgga tttgtacaat ttcacccatc atatgattat
      901 acggattttg tagaaggttt aagaccagta tcaaatgggg atggagctat tgagtttagg
      961 ctacaggacg gtatttttaa agatttttgt cagaaagcaa aagaaaccca attgattgga
     1021 ggacaagata attttgatga ggcttgggat tcttacttag aatatataaa tgttgctgaa
     1081 gaaaaagaat atataacaaa aacatcttac ttatctgtta atagtagaca aaatttgtca
     1141 gtaaattatg atagtggtgt tccaggatgg tcactaccta gcaaatatgt ttacgagttg
     1201 tataaagata aaaattataa taagcaagaa tactacaaaa gtggtggaaa aactgtccta
     1261 gaaacattga gaaagagatt tggtttgaaa gactatgttt ccccaacaga aattgatact
     1321 gataagaaat tcgtcttcat catcgatgaa atcaatcgtg gggagatttc taagattttt
     1381 ggcgaactct ttttctctat cgaccctggc tatcgtggtg aaaaaggaag tgtttctacc
     1441 caatatgcaa atctacacga aactgatgaa aagttctata tccccgaaaa tgtttacatc
     1501 atcggaacta tgaatgatat tgatcgttca gtggatacct ttgattttgc tatgcgtcgt
     1561 cgttttcgtt ttgttgaagt tactgccgag ggtcaagttg gcatgttgga taaagagctg
     1621 aatatccatg cagaagaagc aaaaattcgt ctaagaaact tgaacgctgc tatcgaaaat
     1681 gttcaggaat taaacagtca ttatcatatt ggaccaagtt attttcttaa gttgaaggat
     1741 gtagattttg actatgaatt actctggtct gattatatta agcctctcct agaagactac
     1801 ttgcgaggtt cttatgatga ggttgaaact ttggaaactt tgaaaaaagc atttgaactg
     1861 acaaataatg agcaaaaaga tcaggcagta gctgatgaca atgaaggtga tgaaaacgat
     1921 gatgcggatt actgataatc aacacaagat tattaaagaa aaatttgttg aagaatatcc
     1981 taaactaagc aatcttcttt tagacagaac cttggaaagt ctatcccaag atgaacgtat
     2041 tttcattttt ccaaatgatt tgactcatac tcctgatttg gataaggacc aaaagatttt
     2101 tgaaacagtc aatcagaaaa tcaagacagg gaacgtgatt ggttttcttg gatatggtca
     2161 ggaaagatta acgatttcct cacgattttc tgatgagagt aatgaccatt ttttgcatta
     2221 tctcttaaac aaggttcttc atatcaatct cactagttta gatgttgctt tgtctcgtga
     2281 agagaggctt tatcaacttt tggtgtatct ctttcccaag tatctacaag ctgctattcg
     2341 aaaaggtctt tataaggaat atcatcgatt ttctcataac gacagtcatg ttaagggagt
     2401 gattgatgta agaaaccatc tcaagaaaaa tcttcctttc acgggaaata ttgcctacgc
     2461 aacgagagag ttcacctatg ataatcccct catgcagttg gtccgtcaca ctattgaata
     2521 cattaagaat cagaaaagca ttggtcaagg ggtactagat aatctctcaa ctagtcgtga
     2581 aaacgtatct gaaatcgtgc gtgtaacgcc ctcttataaa ctagctgatc gtgctaagat
     2641 tattcgggga aatcaatcta aacctatacg tcatgcatac tttcacgagt acagaaactt
     2701 acaagaactt tgtctgatga tcctaaacca agaaaagcac ggtttagggt atcaagatca
     2761 aaaaatctat ggtattctct ttgatgttgc ctggctttgg gaagagtatg tttacacctt
     2821 gttgccaaaa ggttttgtac atcccagaaa taaggataag acggatggaa tttcagtatt
     2881 ttctgttggg aaacgaaaag tatatccaga tttttatgac agagaacgaa agattgttct
     2941 agatgcaaaa tataaaaaac tggaattgac tgaaaaagga atcaaccgtg aggacttatt
     3001 ccagctgatt tcctattctt atattttaaa agctgagaag gctggacttg tttttccgag
     3061 caaggacaag gttattgata atgagatggg aaacctagca ggttatggag ctttgttgaa
     3121 gaaatggtct atccaaattc ctcagaagtc ttcatcttat agtgcatttt gtaaaatgat
     3181 ggaaaattca gaagaaaatt ttaaagcgat tattgatgaa gaagtgggga gaaaataatt
     3241 tcctctcttt cttttatttt taacacaagt aaaacccgaa caatatattt caattcataa
     3301 aataatttga caaaaactgt actttggttt ataataaatt aaggaaacgt cggaaaaggc
     3361 gtagtgatgc aagagcatag gtaggtcatt acaaaagaaa cgagacatcg atatgttaaa
     3421 tgaatttcca atttttgatt acgaagatat tcaattgatt ccaaataaat gtgtcattaa
     3481 aagccgtgca gaagcggata caagtgtcac tttaggaaat cacaccttta aactacctgt
     3541 tgtgccagcg aatatgcaga cgattttgga tgaaaatata gcagagcaac tggctaaagg
     3601 tggttacttc tacattatgc accgttttga tgaggcagga cgcattcctt ttattaaacg
     3661 aatgcacgat caagggctca ttgcttccat atctgtcggt gttaaggact atgagtatga
     3721 ttttgttagc cagctcaagg ctgatgctcc tgagtatatc actattgata ttgctcatgg
     3781 tcatgcggat agcgtgattt ctatgattca acacatcaag aaagaattgc cagatacctt
     3841 tgtcattgct ggaaatgtgg gaacaccaga agctgtgcgt gaattggaaa atgctggtgc
     3901 ggatgctact aaggtcggaa tcggtcctgg taaggtttgt atcacaaagg ttaagactgg
     3961 ttttggtaca ggtggttggc agttggctgc tctacgctgg tgtgccaagg ctgcgcgtaa
     4021 accgattatc gctgatggag gaattcgtac tcacggtgat attgccaagt ctatccgctt
     4081 cggtgctagc atgatcatga ttggttccct ctttgcagga cacattgaaa gtccagggaa
     4141 aacgattgaa gtcgatggtg aacagttcaa agaatattat ggttcagcct cacaatatca
     4201 aaaaggtgct tacaaaaatg tggaaggcaa acgtatcttg cttcctgcta aaggtcatct
     4261 gcaagacact ttaactgaga tggaacaaga ccttcaaagt gctatttctt atgcaggtgg
     4321 acgtcaggtt gctgacctta aacacgttga ttatgtgatc gtgaaaaact ccatctggaa
     4381 tggggatgct tcccactaag acggggtctt tgaccaaaaa acttgttatt agagcaaatt
     4441 tctgttataa taaaacaagt ttccaccctt agtgtaatgg atatcacgta agattccggt
     4501 tcttgagatg ggggttcgat tccctcaggg tggatgtaaa catcctaaaa agcctttaat
     4561 agggcttttt tctttatcct gacccaaatt tgccgctaaa actgagaatc tctgtctaaa
     4621 gaattcccaa atgctttcca gtcccgtttt agagtgactc agggggcttt ttttgatata
     4681 ataaaaagga ctattatcag ttagaaagag gttggtatga aagaattaca aactgtacta
     4741 aagaatcatt ttgcaatcga atttgcagac aaaaatttac tggaaactgc ctttactcat
     4801 acgagttatg ccaatgagca ccgcctctta aaaatttcac acaatgaacg cttggaattt
     4861 ttaggagacg ctgttctaca gttattgatt tcagaatatc tatataaaaa atatcctaaa
     4921 aagcctgaag gtgacctatc aaaactccgt gctatgattg tccgtgagga gagtttagct
     4981 ggttttgcgc gtgattgcca gtttgaccag tttatcaagt tgggtaaagg ggaagaaaaa
     5041 tcaggtggtc gcaatcgtga aaccattctt ggtgatgcct ttgaagcctt tcttggtgcc
     5101 cttcttttgg ataaggatgt ggccaaggtc aaggaattta tctaccaagt tatgattcct
     5161 aaggtagaag caggcgaatt tgagatgatt acagactata aaacccatct ccaagagtta
     5221 cttcaggtca atggtgatgt ggctattcgt tatcaggtga tttctgaaac agggcctgct
     5281 cacgataagg tctttgatgt agaagttctt gttgaaggta agagcatcgg tcaaggccaa
     5341 ggtcgttcta agaaattagc agagcaggaa gctgccaaaa atgccgttga gaaggggctg
     5401 gattcatgta tttaaaggaa atcgaaattc aggggttcaa gtcttttgct gataagacca
     5461 aggtcgtttt tgaccaaggt gtgacggcag ttgttggacc caatggatct ggaaagtcca
     5521 atattacaga aagtctgcgt tgggctttgg gggagtctag tgtcaagagt ctccgtgggg
     5581 gcaagatgcc ggatgtcatc tttgctggaa cagaaagtcg caaaccgctc aattatgctt
     5641 ctgtagttgt gactctggat aatcatgacg gatttatcaa ggatgcaggt caagaaatca
     5701 gggtggaacg ccatatctat cgtagtggag atagcgaata caagattgac ggcaagaaag
     5761 tccgtctgcg tgatattcat gacctcttct tggatactgg attgggacga gattccttct
     5821 ctatcatttc ccaagggaag gttgaggaga tttttaattc taagcctgag gaacgccgag
     5881 ctatttttga agaagctgca ggagttttaa aatacaagac tcgcagaaaa gaaaccgaga
     5941 gtaaactgca gcaaactcag gataatctgg accgcttaga ggacattatc tacgagttgg
     6001 ataatcaaat caagcctctt gagaagcaag ctgagaatgc ccgtaagttt ttagacttgg
     6061 aaggacaacg taaggctatt tatttagatg ttctggttgc tcaaatcaag gaaaataagg
     6121 ctgaactaga gtcgacagaa gaagagttgg ctcaggttca agaactcttg atgagttatt
     6181 accaaaagcg tgaaaaatta gaagaagaaa atcaaactct taaaaagcaa cgccaagatt
     6241 tacaggctga aatggccaaa gaccaaggca gtttgatgga cttgactagt ctgattagtg
     6301 atttagaaag aaaattagcc ctatcgaaac tggagtccga gcaagtggcc ttgaatcaac
     6361 aggaggcaca agcccgtttg gctgctttgg aggataagag aaattcactc agcaaagaaa
     6421 agtctgataa agaaagctct ttagctctgt tagagggaaa tctagtccaa aataatcaaa
     6481 aactcaatcg tttagaagct gaattgctgg ctttctcaga cgatcctgat cagatgattg
     6541 agctcttacg tgaacgcttt gtagctcttt tacaagaaga agcggatgtc tcaaaccagt
     6601 tgacccgtat tgagaatgag ttggaaaata gtcgtcagct ttctcaaaaa caagcagatc
     6661 aactagaaaa gctgaaagag caattagcta tagctaaaga gaaggctagt cagcaaaagg
     6721 aagagcttga aactgccaag gagcaggttc agaaattatt ggctgactac caagctattg
     6781 ccaaggagca agaggagcag aaaacttcct atcaagctca acaaagccaa ctctttgacc
     6841 gtctggataa tctcaaaaac aagcaggcca gagctcaaag tttggaaaat atcctgagaa
     6901 atcatagtaa cttttatgca ggtgttaaga gtgttctcca agaaaaagat cgcctaggtg
     6961 ggattattgg tgcagtcagt gagcatctga cctttgatgt ttattatcaa actgccctag
     7021 agattgcctt aggggcaagt agccagcata tcatcgtaga agatgaagag tcggcaacca
     7081 aagctattga tttccttaaa cgaaacagag ccggtcgtgc aaccttcctt ccgttgacaa
     7141 caatcaagga tcgtacgatt tctagccaga atcaagatgc tatcgctgta agtccaggtt
     7201 tccttgggat ggcagatgag ttggtgactt ttgatactag actggaagcc attttcaaga
     7261 atttgctagc tacaacggct atctttgata ccgtggagca tgcgcgtgca gcagcgcgcc
     7321 aggttcgtta tcaggttcgt atggtgacat tggacggaac agagctgcgc acaggtggtt
     7381 cttatgcagg tggagctaat cgccagaata acagtatttt catcaagcca gaactggagc
     7441 aattacaaaa agaaattgct gcagatgaag caagcttggg ttcagaagaa gcggctttga
     7501 agaccttgca agatgagatg gccagattga tcgagtcatt agaagctatt aaatctcaag
     7561 gagagcaggc acgcattcag gagcaaggct tgttcctcgc ttatcagcaa actagtcagc
     7621 aagttgaaga actggaaact ctttggaaac tccaagaaga ggaaatagat cgtctttccg
     7681 agggagattg gcaagcagac aaggaaaaat gccaagaacg ccttgctact atcgccagtg
     7741 acaagcaaaa tctggaagct gagattgaag agattaagtc taacaaaaat gccatccaag
     7801 aacgctatca aaacttgcag gaagagctag cgcaagctcg tttgcttaag acagaactgc
     7861 aagggcaaaa acgttatgaa gttgctgata ttgaacgctt aggcaaggaa ttggacaatc
     7921 ttaatatcga acaagaagaa atccagcgca tgctccaaga aaaagttgac aatcttgaga
     7981 aggttgatac agaattgctc agtcaacagg cggaagaatc caaaactcag aaaacaaatc
     8041 tccaacaagg tttgattcgc aagcagtttg agttggatga tatagaaggt caactggatg
     8101 atattgccag tcacttggat caagctcgcc agcagaatga ggagtggatt cgcaagcaaa
     8161 cacgtgctga agccaagaaa gaaaaggtca gcgagcgctt gcgccatcta caaaatcaat
     8221 taacagacca gtaccagatt agctatactg aagcactaga aaaggcacat gaattggaaa
     8281 acctcaatct ggcagagcaa gaggtgcagg atttagagaa ggctattcgc tcattgggac
     8341 ctgtcaactt ggaagctatt gaccagtacg aagaagttca caaccgtctg gactttctaa
     8401 atagtcagcg agatgatatt ttgtcagcga aaaatctgct ccttgaaacc attacagaga
     8461 tgaatgatga ggtcaaggaa cgctttaaat caacctttga agctattcgt gagtccttta
     8521 aagtgacctt caagcagatg tttggcggag gtcaggcaga cttgatattg actgagggcg
     8581 accttttaac agctggtgtg gagatttctg ttcaacctcc aggtaagaaa atccagtcgc
     8641 ttaacctcat gagtggtggt gaaaaagctc tatcggctct tgccttgctt ttctccatta
     8701 ttcgtgtcaa gaccattcct tttgtcatct tggatgaggt ggaagctgcg ctggatgaag
     8761 ccaatgttaa acgttttggg gattacctca accgctttga caaggacagc cagtttatcg
     8821 tcgtaaccca ccgtaaggga accatggcag cggctgattc catctatgga gtgaccatgc
     8881 aagaatcggg tgtttcaaag attgtttcag ttaagttaaa agatttagaa agtattgaag
     8941 gatgacaatt aaactagtag caacggatat ggacggaacc ttcctagatg agaatgggcg
     9001 ctttgatatg gaccgcctca agtctctctt ggtttcctac aaggaaaaag ggatttactt
     9061 tgcggtggct tcgggtcggg gatttctgtc tctggaaaaa ttatttgctg gtgttcgtga
     9121 tgacattatt ttcatcgcgg aaaatggcag tttggtagag tatcaaggtc aggacttgta
     9181 tgaagcgact atgtctcgtg acttttatct ggcaactttt gaaaagctga aaacgtcacc
     9241 ttatatagat atcaataaac tgctcttgac gggtaagaag ggttcatatg ttctagatac
     9301 ggttgatgag acctatttga aagtgagtca gcattataat gaaaatatcc aaaaagtagc
     9361 gagtttggaa gatatcacag atgacatttt caaatttaca accaacttca cagaagaaac
     9421 gctagaagct ggtgaagctt gggtcaatga taatgtccct ggtgtcaagg ctatgacaac
     9481 tggctttgaa tctattgata ttgttctgga ctatgtcgat aagggtgtag ctattgttga
     9541 attagctaaa aaacttggca tcacaatgga tcaggtcatg gcttttggag acaatcttaa
     9601 tgacttacat atgatgcagg ttgtgggaca tcctgtagct cctgaaaatg cacgaccaga
     9661 gattttagaa ttagcaaaga ctgtgattgg tcaccataag gaccagtcgg ttatagctta
     9721 tatggagggc ttataatggc agatataaaa ttaatcgcat tggacttgga cggtaccttg
     9781 ctgactactg ataaaaggct gacggatcgc accaaggcaa ctttgaaagc tgcgcgtgat
     9841 cgtggtatca aggtcgtatt gacaactggt cgtcccttaa aagccatgga cttctttctc
     9901 catgagttag ggactgacgg tcaggaagat gagtatacca ttacttttaa tggtggtttg
     9961 gttcagaaaa atacaggtga aatccttgat aaaacagtct tttcatatgc tgatgtggca
    10021 cgtttgtatg aagaaacaga gaaattatca ctgccacttg atgccatctc agaaggaaca
    10081 gtttatcaaa tccaatcgga ccaagaaagt ctttatgcca aattcaatcc agctttgact
    10141 tttgttccag ttgactttga agacctgtct agtcaaatga cctacaacaa atgcgtgact
    10201 gcctttgctc aagaaccctt ggatgcagcc attcagaaga tttctccaga attgtttgac
    10261 caatatgaaa tctttaaatc acgtgaaatg ttgctagagt ggtcaccaaa gaatgttcat
    10321 aaagcaacag gtttggcaaa actaatcagc catcttggaa tcaaccaaag ccaagttatg
    10381 gcctgtggtg acgaggccaa tgacctttct atgattgaat gggcaggtct tggtgttgct
    10441 atgcaaaacg ctgttcctga agtaaaggca gccgcaaatg tagtaacgcc gatgaccaac
    10501 gatgaggaag ctgtcgcctg ggctatcgaa gaatatgtgc taaaggagaa ctaagatatg
    10561 ggattgtttg accgtctatt cggaaaaaaa gaagaaccta aaatcgaaga agttgtaaaa
    10621 gaagctctgg aaaatcttga tttgtctgaa gatgttgatc ctaccttcac agaagttgag
    10681 gaagttcctc aggaagaagc agaggttgaa attgttgaac aagctgtgtt ccaagaggag
    10741 gaaatccaag acacagttga agaaagtcag gattcagagc cagctgtagg ggtttctcaa
    10801 gaagaaatag aagaattacc aaactcagaa gaattaccag aggaagagaa gcttgagcac
    10861 gaaggaatta tagaagaaaa taattctgaa gttcttgaac cagaaaggcc tcaaacagaa
    10921 gaaactgttc aggaaaaata tgaccgcagt ctcaagaaga ctcgtacggg cttcggtgct
    10981 cgcttgaacg ccttctttgc taacttccgc tctgttgacg aagaattttt cgaggaactg
    11041 gaagaactgc tgattatgag tgatgttggt gtccaagtcg cttctaactt aacggaggaa
    11101 ctacgttacg aagccaagct tgaaaatgcc aagaaacctg atgcacttcg tcgtgtcatc
    11161 attgagaaat tggttgagct ttatgaaaag gatggtagct acgatgaaag catccacttc
    11221 caagataact tgacagttat gctctttgtt ggtgtgaatg gtgttgggaa aacaacttct
    11281 attggaaaac tagcccaccg ctacaaacga actggtaaga aggtcatgct ggttgcagca
    11341 gataccttcc gtgcgggtgc agtagctcag ctagctgaat ggggccgacg agtagatgtt
    11401 ccagtagtaa ctggacctga aaaagctgat ccagccagcg tggtctttga tggtatggaa
    11461 cgtgccgtgg ctgaaggtat cgatattctc atgattgata ctgctggtcg tctgcaaaat
    11521 aaggataacc ttatggctga gttggaaaag attggtcgta ttatcaaacg tgttgtgcca
    11581 gaagcaccac atgaaacctt cttggcactt gatgcatcaa caggtcaaaa tgccctagta
    11641 caggccaaag aattttcgaa aatcacacct ttaacgggaa ttgttttgac taagattgat
    11701 ggaactgctc gaggaggtgt ggttctagcc attcgtgaag aactcaatat tcctgtaaaa
    11761 ttgattggtt ttggtgaaaa aatcgatgat attggagagt ttaactcaga aaactttatg
    11821 aaaggtctct tggaaggctt aatctaatca gaagcaaaaa tcctgcaagg tacaaacttg
    11881 caggattttt ttattcgaaa cgaccatctt gacgataggc gatatctggt tgccaagtcc
    11941 atttggcatc gaatttttca agtaggtcaa agctggcttg aggtcccatg cttccagctt
    12001 tatagtcatg aagtggggca ccattttcgg cccagagctc ttcaatacgg tcaatcaact
    12061 tccatgacgc accaacctca tcccagtggc taaagttagt tgagttgtta tttaggacat
    12121 cataaatcaa tttctcgtat ggttctggag aagcaccagt tgcagtcgcg tctgtacgat
    12181 aatcaagtga gttaggagcc aagttaaatt cttctcctac ttgcttccca tttaggctaa
    12241 gagagaagcc ttctgttggt tgaatataga tggtcaaaat atttggagca agtggttctc
    12301 caaagataga atccatttgt ttaaagacga tgttgacatg agttcctttt tcagtcagtc
    12361 gtttacctgt acggaaaaag aaaggaacac cacggaatcg atcgctgtct acaaagaagg
    12421 caccagatgt aaaggtttca gttgttgatt ctggattcac atttggttcg ctacgataag
    12481 agatgtattt catgccatca atcttaccag agcggtattg tccacggata aagtgttctt
    12541 tgagttcttc atcagttgga tgatagaggt ttttaaagac cttaatcttt tcagcacgaa
    12601 tctcgtcttt tgtgaagctt gctggtttgt ccatggcgag gagcgaaaga agttgtagag
    12661 tgtggttttg gaccatgtca cggagggcac cggattggtc atagtagcca ccacgttctt
    12721 ctacacccaa gcgctccgca aaggtaattt gaacattgtc gataaaatcc ttgttccaaa
    12781 cgttttcaaa aatcaagttt gcaaagcgaa ctgcaaagat gctttggatc atttccttac
    12841 caagataatg gtcaatacgg aaaatttgtt cttcgtcaaa tgttgctagg agttcgtcat
    12901 tcaacttact tgcagttgcg taatctgtac caaatggttt ttcaacgatc aagcgctcaa
    12961 aacctttgcc atcgacaatg ttttcagatt tgaggtgttt ggcaatggtt ccaaagaact
    13021 gaggtgccat agacaagaag aagagcttat tgtgttcagc ttggtatttt tcattaagct
    13081 cagcttgtaa ttgacgcaaa gcaatataat gttccgaatc attgacatca tggctttgat
    13141 agtagaagtg gctagcaaat tcttgggctt gctcggtact atctgccaaa tcaaggatgg
    13201 actcgacaac tacagattca aaatattcct tactccaagg tctacgggca gttccaataa
    13261 cggcaaagtg cttggaaaga ttgccggatt tatatagtct aaaaagggaa gggtagagct
    13321 tgcgtttagc caggtctcca ctcgcaccga aaattgtaac aataacctta gatgacatct
    13381 agctacctaa tttctatttt tattcctagt cttgctaggt atgaggaaac ctcatttcat
    13441 aaacttaatt ctaacataat tttccgaaaa atcaaaaatt ccaatacgag gtagaaaaac
    13501 tgcaaggaaa tccttacagt ttgagtttag acgtttaaga ccttatctaa gaactctttc
    13561 agacgagggt gttgtgggtt atcaaagatt tggtcaggtg ttccgtcttc aaggaactcg
    13621 ccatctgcag taaagataac gcggttggca acctgacgag caaatcccat ctcatgggtt
    13681 acgataatca tggtcatgcc ttgctcagcc aattccttca taacgttaag tacgtctcca
    13741 accatctcag ggtcaagggc agaagttggt tcatcgaaga gcatgatgtc tggattcatt
    13801 gctaggccac gagcgatggc cacacgttgt ttttgaccac ctgataggct atctggattg
    13861 gcattagctt tatctgctag tccaaccttt tcaagcaact ccattcccaa ttcctcagct
    13921 tcttccttag tcatcaactt gtgctcaata ggagcaaagg tgatgttgtc caatacagac
    13981 atatgaggga agaggttgaa gtgttggaat accatgccga tattttcacg gacgtggtca
    14041 acattggttg ttttttcagt taaatcatag ccgttcacag tgatgtgacc gctagtgact
    14101 tcttctaaaa gattgaggct acggaggaaa gttgacttac cagaacctga aggaccgatg
    14161 atacaaacaa catctccttc atagaactta gtcgtaattc cttttaggac ttcatttttt
    14221 ccatagtgct tgtgtaaatc atttacatca atttttaatt ttgccattaa cgaatcctct
    14281 tttctaagcg tttcgctagt ctagtcaaaa gtgtgataat tacaagatag aagatagcaa
    14341 ggattgcata catcttgaaa ctttggtagt tacgagcaat gataatctta ccagtttgga
    14401 agagttcaac caaaccgata gcagatacga tagttgtatc tttaagagcg ataacgaatt
    14461 ggttgacaaa gtttggcaac atcaatttag ttgcttgtgg caagataatc ttacgcatgg
    14521 tttttccata agagataccc aagcttcggc tggcttccat ttggccaact ggaacggcct
    14581 gaataccacc acgaacgatt tcagcgatat aagcagccgc attgagtgag agggcaattg
    14641 ttccagctac aaagtcgtta attgggcttt gttggcctgt gatagactcg atgaagtttg
    14701 gaattcccca gaagatgaag gctgcaagaa tcatcaatgg aataccacga ataacgtcaa
    14761 cgaaaatctc agagatgacg cgaagagatt tgtatgggct aacgctaaac ataccgaaga
    14821 taattccgat gacaatggca atagcaaatg agataagagc tagagcaaga gtgataccaa
    14881 gaccgctaag gagttgtttg tagttgtttt gaagcaagcc ccagagcgtt gtttcgtcaa
    14941 cagtacttgt tgaagcagtt gaagattcgc tagctaggta tttgtcaaga atcttttgga
    15001 attcaccgtt tgctttaagg tttgcaagtc cgttgttgaa catttcaatc agttctggat
    15061 ttgctccttt tttaacggca aaggctgttt caccgattgg agttccagag attggagttt
    15121 tcaatttttg actttggctg atagaatatt tgagaacagg ttcatcatcc ataacggcat
    15181 caatggcacc agtgtttaaa ctgtcataca ttgaagaacc atcagcaaag gttttgattt
    15241 tgtaaccgta tttgctttga ttttctgtta ggaaggtttg agaagcagtt ccgtttttaa
    15301 caccgactgt ctttcctttt agatcttcat aagaagcaat attgcttgat tctttgacac
    15361 caagaatggt attagcagtg tagtatgatt ctgagaagtc aaaagttgcc ttacgagcat
    15421 ctgtgacaga cataccagca atgataccat cggcttgacc agcttggaca gcactgatag
    15481 cagcatcaaa accagggttg gtgatttcaa tttcaaaacc ttggtcttta gcgattgcct
    15541 taatcaattc catatcaata ccagtgtatt ggttgcttga attttggaaa acaaaaggtg
    15601 caaaagaaga atcgctggca atgatatatt tagccttaac aggaatagct tttaatcctg
    15661 cgagagtagt tgtagttggc actgctgaag atgatgaagc agtccatttc ttgataattt
    15721 tatcaagact accatctttt ttcatttcag acaaggcttg gttaaattca gtaaccaggt
    15781 gctcgtattt acttcctttt ttcacaccga aagcaaaact tcctacagct tcaccatcca
    15841 tttcaatatg gaggtcttga ccttggttaa tggcatattc gataacaggt ttgtcatcca
    15901 tcatggcatc gatggcacca gcactcaagc tgttgttcat taaatcacca gtgtcaaatg
    15961 ttttaatagt aaagccgtat ttatctttga ttgtttcaag gaaacgttga gcggcagttc
    16021 cgtttttaac accaacggtt ttgccagtta attggtcgta cttgctaatt ttgtgtgact
    16081 ttgtagtagc aatgacaact tttgtatcat agtaagtatc agacatggtg aagacttttt
    16141 cacgttcttt agtctttgtc atccctgcca tgatagcgtc ggcttgccca gcttgaaccg
    16201 cattgactgc tgcgtcaaat ccaggatagg acatctgaat gttccagcct ttaatctcag
    16261 cgactttgtt aataatgtca acatcaattc ctttataagt ttgatctgaa tctttaaact
    16321 caaaaggtgc ataggcggta tcagaaacaa tcttaatcgt ttctgctttg gcaataccta
    16381 atgagaaaat tgggaataaa attagcaaaa atgctagaaa ttttttcttc atttttagtc
    16441 tccttttccg aatattctct cattatatca gaaaaagtaa aaaaaggcaa atttttatat
    16501 ttttcgtgtc agaaaatata acattgattt tttcctttcc ttgaattgct attaa
//
LOCUS       DAVMAF010000038        15941 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMAF010000038 DAVMAF010000000
VERSION     DAVMAF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.038"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..115)
                     /locus_tag="VYO97_001281"
     CDS             complement(<1..115)
                     /locus_tag="VYO97_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1754071.1"
                     /translation="MNYEASKQLTDARFKRLVSVQRTTFEEMLAVLKTAYQK"
     gene            240..698
                     /locus_tag="VYO97_001282"
     CDS             240..698
                     /locus_tag="VYO97_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1754072.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            711..1319
                     /locus_tag="VYO97_001283"
     CDS             711..1319
                     /locus_tag="VYO97_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754073.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSRLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1390..1722
                     /locus_tag="VYO97_001284"
     CDS             1390..1722
                     /locus_tag="VYO97_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754074.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1733..3148
                     /gene="ply"
                     /locus_tag="VYO97_001285"
     CDS             1733..3148
                     /gene="ply"
                     /locus_tag="VYO97_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1754075.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4029..4745
                     /locus_tag="VYO97_001286"
     CDS             4029..4745
                     /locus_tag="VYO97_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1754076.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4869..5318
                     /locus_tag="VYO97_001287"
     CDS             4869..5318
                     /locus_tag="VYO97_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1754077.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNCLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            5357..6178
                     /locus_tag="VYO97_001288"
     CDS             5357..6178
                     /locus_tag="VYO97_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754078.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQETNLDELWQPA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGSIMAIWAIGKILGHSEYWLWAVLVVVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            6185..7216
                     /locus_tag="VYO97_001289"
     CDS             6185..7216
                     /locus_tag="VYO97_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754079.1"
                     /translation="MDFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPAQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSIGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIACALLKDASLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSIIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7281..7643
                     /locus_tag="VYO97_001290"
     CDS             7281..7643
                     /locus_tag="VYO97_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEV1754080.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGEKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERITITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7640..8143
                     /locus_tag="VYO97_001291"
     CDS             7640..8143
                     /locus_tag="VYO97_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1754081.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSAGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8257..8703
                     /locus_tag="VYO97_001292"
     CDS             8257..8703
                     /locus_tag="VYO97_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1754082.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8708..9382
                     /locus_tag="VYO97_001293"
     CDS             8708..9382
                     /locus_tag="VYO97_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754083.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGTAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            9638..9826
                     /locus_tag="VYO97_001294"
                     /pseudo
     CDS             9638..9826
                     /locus_tag="VYO97_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            10076..10375
                     /locus_tag="VYO97_001295"
     CDS             10076..10375
                     /locus_tag="VYO97_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1754084.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10372..10689
                     /locus_tag="VYO97_001296"
     CDS             10372..10689
                     /locus_tag="VYO97_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1754085.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     SNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            10705..11331
                     /locus_tag="VYO97_001297"
     CDS             10705..11331
                     /locus_tag="VYO97_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1754086.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKDKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11364..12125
                     /locus_tag="VYO97_001298"
     CDS             11364..12125
                     /locus_tag="VYO97_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1754087.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADIVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12203..12598
                     /locus_tag="VYO97_001299"
     CDS             12203..12598
                     /locus_tag="VYO97_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1754088.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12754..13038
                     /gene="groES"
                     /locus_tag="VYO97_001300"
     CDS             12754..13038
                     /gene="groES"
                     /locus_tag="VYO97_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1754089.1"
                     /translation="MLKPLGDRVLLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13054..14676
                     /gene="groL"
                     /locus_tag="VYO97_001301"
     CDS             13054..14676
                     /gene="groL"
                     /locus_tag="VYO97_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1754090.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            14814..15569
                     /locus_tag="VYO97_001302"
                     /pseudo
     CDS             14814..15569
                     /locus_tag="VYO97_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4789 a         2880 c         3648 g         4624 t
ORIGIN      
        1 ttttttgata agctgttttt aatacagcta acatctcttc aaaagtggta cgctgaacac
       61 taacaagacg cttaaatcgt gcatcagtta gttgtttact tgcttcataa ttcatagaac
      121 tactatacca tattttgttt cgcaggaagt ctaataagtt atagtccagt ttttactata
      181 ctttgatatt caaatatgtt atactatggt tatcaaagta aatgaaagga gttacgaata
      241 tgacgccaga agaaatgtac ctgacagagc gattagacgt acagatagct cattttttaa
      301 agaaaagcgt tcaacatcgt agacgctata aggtattaaa aataacagaa atcgtggcag
      361 gtttcctcat agctgtcttt tgtgctattc ctatgccagg tgatcgctac cgtttgattt
      421 cggttgcctt atccagtctc ggcttgctgt gtgaggggat tatcaatttg tataatgcaa
      481 aggaaaattg gatttcttac caaaaaactg cgcaactcct ggagaaagaa aaattcctct
      541 atcaatgcca aacggagaaa tatgcaggaa agaccaaggc ttttgcccta tttgtcaaga
      601 catgcgaagg tcttatctca gaggagatta accagtggga aagtatccag tcaaaagaag
      661 tggcagctag tgcagatgct ccagttaaaa aagagtagga ggtagaggaa atgtctcaat
      721 ccagctacct gtcgcccttg ctctggttaa aaaaagaagc cgataaggaa aagatgagcg
      781 cgacccagtg ccagatattt ttcttttact atcaaatgtt tgagctctta tttgctagag
      841 aaagcgacat gaaagactta tgtctgggaa cgaaaggttt ttatttctcg cagttagaga
      901 aaaatttgct ttctggagtt tcccgatttc taaaaaactt ggaggggaaa gtaactctca
      961 aggctaacca agaagtatca gctcgcaaag ccctttttct agccttgaca actagccaat
     1021 cagattggca ggagttagct cctgtttttg atttttatca gactatcggg aggcttgaaa
     1081 atccttctcg cttgagttct caggacagac aacatctgat gtggatttac cagtcagctt
     1141 tggagaagga ttatattgtc aaggttattg gcgacaagca ttttgtattg aagagacaag
     1201 atgctactaa attgacagcg cgccaaactc aaactttgga aattctgagt caatcagaag
     1261 acttggtcaa tcctgtctat gttacattag gagaaaaggg ggtgctcttg cttgattaag
     1321 agaggagatg ttgtagctct ttatttgcct tttccgacta ttagtagcga tttggctgtg
     1381 aagaatcata tgtatatctg tattgacaac agcatgacta aaaacaaaga gttggttaaa
     1441 aatcagacct tcaaaccagc tctcttgacc agacgtttgg tcaagaactt tatgatagaa
     1501 gagccggatc tagctcgtaa tccttttaca agaccaacct tgattgactt agataaggta
     1561 tttatgttgg ataatacggt tattccgact tcttatctag ccagacggcg acgcaatgtc
     1621 tcagaagaat tgtacgagga aattttggat tacttagtcc aaccacggct gatttcgctg
     1681 aacaagtctg agtttatgca actcaatcca ggaacttatt aggaggtaga agatggcaaa
     1741 taaagcagta aatgacttta tactagctat gaattacgat aaaaagaaac tcttgaccca
     1801 tcagggagaa agtattgaaa atcgtttcat caaagagggt aatcagctac ccgatgagtt
     1861 tgttgttatc gaaagaaaga agcggagctt gtcgacaaat acaagtgata tttctgtaac
     1921 agctaccaac gacagtcgcc tctatcctgg agcacttctc gtagtggatg agaccttgtt
     1981 agagaataat cccactcttc ttgcggtcga tcgtgctccg atgacttata gtattgattt
     2041 gcctggtttg gcaagtagcg atagctttct ccaagtggaa gaccccagca attcaagtgt
     2101 tcgcggagcg gtaaacgatt tgttggctaa gtggcatcaa gattatggtc aggtcaataa
     2161 tgtcccagct agaatgcagt atgaaaaaat cacggctcac agcatggaac aactcaaggt
     2221 caagtttggt tctgactttg aaaagacagg gaattctctt gatattgatt ttaactctgt
     2281 ccattcaggc gaaaagcaga ttcagattgt taattttaag cagatttatt atacagtcag
     2341 cgtagatgct gttaaaaatc caggagatgt gtttcaagat actgtaacgg tagaggattt
     2401 aaaacagaga ggaatttctg cagagcgtcc tttggtctat atttcgagtg ttgcttatgg
     2461 gcgccaagtc tatctcaagt tggaaaccac gagtaagagt gatgaagtag aggctgcttt
     2521 tgaagctttg ataaaaggag tcaaggtagc tcctcagaca gagtggaagc agattttgga
     2581 caatacagaa gtgaaggcgg ttattttagg gggcgaccca agttcgggtg cccgagttgt
     2641 aacaggcaag gtggatatgg tagaggactt gattcaagaa ggcagtcgct ttacagcaga
     2701 tcatccaggc ttgccgattt cctatacaac ttctttttta cgtgacaatg tagttgcgac
     2761 ctttcaaaat agtacagact atgttgagac taaggttaca gcttacagaa acggagattt
     2821 actgctggat catagtggtg cctatgttgc ccaatattat attacttgga atgaattatc
     2881 ctatgatcat caaggtaagg aagtcttgac tcctaaggct tgggacagaa atgggcagga
     2941 tttaacggct cactttacca ctagtattcc tttaaaaggg aatgttcgta atctctctgt
     3001 caaaattaga gagtgtactg ggcttgcctg ggaatggtgg cgtacggttt atgaaaaaac
     3061 cgatttgcca ctagtgcgta agcggacgat ttctatttgg ggaacaactc tctatcctca
     3121 ggtagaagat aaggtagaaa atgactagga gaagagaatg cttgcgacaa aaagaggcga
     3181 tgatctctct gcggatatct gaaagtttat ctcttgccta gcgatttcca ttggagtatg
     3241 ctcgtgatgt taaaatgagt ctatcaaaca ggatgtgtaa aaaagtaaaa agggtagcca
     3301 attccttgat atacagggga ttgattatcc tttacttgtt ttagatgatt ggaaattcaa
     3361 gaatgacacg tatcggaaat attttgctgt ttatatctaa tacccattta tggaaaaaat
     3421 gaaattaaat atgaacaaat caattgctag caatggttta gaagtgccag tgtactagtc
     3481 taacttctcc atcttgggaa cacgtaaatt atactcttcg aaaatctctt caaaccaggt
     3541 cagccctatc cgcaacttca aaacagtgtt ttgagcactc tgcggctagc tttctggttt
     3601 gttctttgat ttttattgag tattagaggt ttgcttagaa actcttctat tgatttctta
     3661 gaatacaata gcgcctataa gaactgttgg atttcaaagt atttacttga actatgatat
     3721 gtctggagta gactgtcaac aaaaggtgca gtatgattgt ttttgtcgct tatatctaca
     3781 aacttcaaac agtaatttga gctgactttg tcaaacttgg ctgaaacttt ctatttgcag
     3841 ataatggtct agtttgtttt ttgaatgtgg atgagtgtct gttgataaag atagaaaggc
     3901 aagaccgtct tagcaggtaa agtgctatcc tagcttgatt tgctaataaa atccatttcc
     3961 tctagtgaaa atcaaaaaaa cttgtgttat aataagaaag attaaaatgt gaaaaaagga
     4021 gattcctaat gggacgtaaa tgggccaata tcgtagctaa gaaaacggct aaagatggag
     4081 ccaactctaa agtatatgca aaatttggtg tagaaatcta tgtagcagct aaaaaaggtg
     4141 atccagatcc agaatcaaac tcagctttga aattcgttat cgaccgtgct aaacaagccc
     4201 aagtgccaaa acacattatc gataaagcga ttgataaagc caaaggaaac acagacgaaa
     4261 cctttacaga aggacgttac gaaggttttg ggccaaatgg ctctatgcta attgtggata
     4321 ctttgacttc taacgtcaac cgtacagcag ccaatgtccg tgcagccttt ggtaaaaacg
     4381 gcggaaacat gggcgcttca ggttctgttt cttacctttt tgacaacaaa ggtgttattg
     4441 tatttggagg tgaagatgcg gacgcagtct ttgagcaatt gctcgaagcg gatgtggatg
     4501 tggatgacgt agaagcacaa gaaggtacaa tcacagttta cacagcgcca actgaccttc
     4561 acaaggctat cgttgctctc cgtgagtctg gtattgaaga attccaagtg actgaattgg
     4621 aaatgattcc tcagtcagaa gtggaattgt caggcgaaga ccttgaaacc tttgaaaaac
     4681 tttacagcgt tcttgaagac gacgaagacg tccaaaagat ttatacgaac gtagatgggt
     4741 tttaatttaa cctatagaac atgatcctaa gtgagaaaat cacttgggat ttttttaatc
     4801 aagaaaatag ttctcataga aatttaatta ttctccagag aactattttt atttagaagg
     4861 gggagattat gggtaaaccg atgttagtct ttaagcgttt tggtcatcaa attcacctga
     4921 tggtgcaaaa ggaagccaaa cgttgcggca ttgaatttat gggtgggccg caaggtcagg
     4981 ttgtgcattt tttggataat cgcgagaaaa accaagactt ggtcttgatt aaagatatcg
     5041 atcaggaact caatattacc aagtctgttg ctagtaatct ggttaagcgt atagtgcaaa
     5101 attgtttggt ggaattggag gcgagtcctg ttgataagcg ggctaagttt gttcgtctga
     5161 cggacaaagc acgttctcag atgcaacagg ttaaggcttt ttttgaacgc atagacaagc
     5221 agttgatgga agatattgat gaagatgaat tactgatttt tgagaaggtt ctcggtcaac
     5281 tacaggcaaa atatcaaggg aataggagga gagaataaag aaattagcca aaagaattag
     5341 tagaaaagaa tgggggatga ttttactagc cattctcttt acttgctttt cggtctatct
     5401 agagttggaa gtgccgacct atatctcgaa aattacggat ttgctaggta gtcaagaaac
     5461 taatttagat gagttgtggc agccggcaag catgatgatg ggaatgtcct ttcttgcctt
     5521 cttgtccgta gttgcagttg gattttttgc atcccgagtg gcggcttctt atactagtag
     5581 gctgagaagt gatattttta accgagtttt ggattactcg cagacagaga ttaagaaatt
     5641 ttcaattcct agcctcttga cgcgtactac caatgacatt actcaagttc aaatgttgat
     5701 tactatgggc ttgcaagtgg taacgcgtgg ttcaattatg gctatctggg ctattgggaa
     5761 gattttaggt cattcagaat actggctctg ggccgtactt gtggtagtga ttgtcaacgt
     5821 cctgatgacg accgttttga tgacgctagc ctttccaaaa cagtccttga ttcaggggtt
     5881 gacagataaa ctgaacagta tcactcgtga gagtttaaca ggtattcgtg tcgttcgtgc
     5941 ctacaatgca gaggattatc aaaatgaaaa atttgcagca gtaaatgatg aattgacccg
     6001 tttgaatttg tttgtcaacc gtcttatggc tattttgaat cctatcatga tggggatttc
     6061 aagtggtttg agtgtagcga tttactggat tggggcctat gtgattaacg acgctgctcc
     6121 gatagcgcgt ctgcctctct ttagtgacat gattgttttc atgtcttatg ccatgtaggt
     6181 tgtcatggac ttccttctca tgggagcact cttcatcgtt cttccccgaa ctatggtctc
     6241 tgctaagcgg attaatcaag ttttagattt gcattcttct atccaaaacc ctgctcaagt
     6301 gcagctgact gatgaaaact tcaaaggtca ggtcgagttt aaggatgtga ccttccgcta
     6361 tgcggcaaat tcggaggcag ttattgaaca tgttagcttt aaagcagaaa ctggtcaaac
     6421 agtggccttt attgggtcaa taggttctgg taaatcaact ctggtcaatc tgattccacg
     6481 tttctacgac gtgtcagcag gagaaattct ggtggacggt gtcaatgttc aagactatga
     6541 cttctctgcg acagctcatg ctggtcaaag ggttgccatt gttgggccga ctggggctgg
     6601 taagacaacc attgtcaatc ttttgatgaa attctatgag attgataagg gaagtattcg
     6661 cattgatggt gtggatacca aggctatgac gcgttcagaa gtgcatgatg ccttttcaat
     6721 ggtcttgcag gatacctggc tctttgaagg aactattcga gacaatctca tctataatca
     6781 aatagggatt agtgatgaac gaatgatgga agctagtaag gctgtgggaa ttcaccactt
     6841 tattatgacc ttgccagatg gctatgatac catcttggat gacaccgtga ccttgtctgt
     6901 aggacaaaaa caactattga ctattgcttg tgcccttctt aaggatgcat cgcttttgat
     6961 tttggatgag gcgacttctt ctgttgacac acggacagag gaattgatcc aaaaagccat
     7021 ggaccgtttg atggaaggac gcacatcctt tgtcattgcc caccgcttgt caatcatccg
     7081 aaatgcagac ttgatcttgg tcatgaaaga tggaaatatc atcgagcaag gcaactatga
     7141 ggaactgatg gcgcaaggtg gcttctacgc tgacttgtac aatagtcaat ttacagaaga
     7201 tgaagcagaa gaataaatca aaaaaggctt gacggccttc tttttcttat ttatactagt
     7261 acacatagaa agagagaagt atgagtcaga aattatacaa tatgaaattt gctgctgtct
     7321 acttagcctt gattgcaaaa gttgagcgaa agggtgaaaa ggcagagtca gttcaccaag
     7381 tgaccagttg gttgacaggg tatgaagtga gtgacgttct tgcttgtctg gatagggatg
     7441 ttacttacgg agattttttt ctacaagcac catattatgt ccctgaacga attacaatta
     7501 cagggaagat ttgcggtgtc cgtattgagg aaattgatga tcctctgatg caggaaatac
     7561 gtcgcttgga caagctagtg gattggcttg ccaaggggaa aacatctcaa caagttctag
     7621 aaaagtatga gaagcataaa tgaaaaatta tcaagaatgg tatcgaaata tcagctccag
     7681 actaactagt cgtccaattc ttttattcct attacgcagt ttcaatcgtt tgatgacagt
     7741 cgccatgccc ctggtctatc tgatcttgct agtcaccact tacctgcaac taggacttgg
     7801 tcagcaagtt ggggtttatg tgcttattcc tgcatcaggt tttgtggcct tgtccctgtt
     7861 tcgtaagaaa atcaatcacc cgagacctta tgaaacttgg gatatctgtc cccttcttga
     7921 taaggatagt gcgggacggt cgatgcccag tcgccatgtc ttttctgcaa ctatcatctc
     7981 catggcctgt ctgcatgcta gtctacctat tggcttagta tgcttgatct tttcagcttt
     8041 gctggggttg gtgagggttt tagggggtgt tcattatccc aaggatgtct tggttggcta
     8101 tgcttgtggt ctggtgtggg gattcctttt cttcctattc tgacatgtaa gttaagctag
     8161 tcctacaacc acttactgct tcaaattgac cacttgcttt tttgcccttg tattcctaac
     8221 ttagctttga tatagtagag tcagaaagga gatgtgatga agttacgaat tgagattgac
     8281 ggcaatttag aggagactga aattgtcatc aagaccccca ctttgacaga tgaaattgca
     8341 gacttgcaac ggctcttgca agagtcaaag gctccgaggt tgacttttta caaggggaca
     8401 ggtgaatatt atctagacct gtcagaaatt ctcttctttg aaacagaagg gagcaagatc
     8461 tacgctcata accagaagga agcttatgag gttcgcctca agctctatga gttggagtct
     8521 atcttgcctc gctattttaa tcgagtttcc aagtcaacga tcgcaaacat ccgtcagatt
     8581 tactcagtgg acaagtcctt ttcaggaacg ggcaccattt ccttttatca gacgcacaag
     8641 gaggttcatg tctcacggca ttaccaatcc ctcctaaaag aaaatctaag aaacatgagg
     8701 taataagatg aaaaagaaag catttggtat tgtgttactt gtactagcag ctttaatatt
     8761 agtacaagga aattttggaa tcccttctct aggagggcac atttggccct tgattggtat
     8821 tgcttttttt gcctaccaat cagttggagc tttgttgcgt cgccacttaa cttcagcctc
     8881 ttttacagct ctagtagcct taatgattgc taaccacttt tatggcattt taccgattcc
     8941 aaaccagaca ctaatctggg caggagttct aatcgttctc ggagtaaata tgttgactca
     9001 ttctaacaga acttggaatg ggaaaaaatg gtggtatgat ggtgaaaaaa acattcttat
     9061 agataaggaa gttgcttttg ggacagggac tttctacaag caagaccaag aattggtaga
     9121 agaccaagta gaagttggtt ttggcaatgc aaaaatctac tatgacaatg cagagatgtt
     9181 aggtgatttt gcaactttaa atattgaagt agcttttggt aatgcaactg tctatgttcc
     9241 gcaacactgg cgtgtcgatt tgaaagtaga aacatccttt ggtacagcca aggcggacgc
     9301 tcctgtagcg ccaacaagca aaaccttgat tattcgtgga gacgtggcct ttgggaagtt
     9361 ggagcttgtc tacgttaaat aaaaaaatct tcacttaaac catcaaaata gacgtactaa
     9421 gagtaggaaa ttgatgcctt gctctgattt cagttctatg gttgttagac tttaaaaaat
     9481 gaaatgttgc ctttaaaagt tgtatatttt tcgatatttt gactcttacg tttgatgtat
     9541 ctatgtccta cagcgtggat gatgtagtgt aaaatgcttt aaaaaaaaaa tgaatgatat
     9601 tggacagttt ttttgaactt ttaattgctc aggagccatg aaagcaagcg cttgggttta
     9661 tgacaaggga gaatggtatt atgtaagttc ttctggttcc atgattgcga atgattgggt
     9721 caaagacaat ggcaagtagt attatctggc ttcatcaggt aagatgcttc gcaataccta
     9781 cacacctgat agttactacg ttggcaactt aggtgcctgg caataagaat aatactcttc
     9841 gaaaatctct tcaaaccacg tcagcttcat ccacaacctc aaaacagtgt tttgagctga
     9901 cttcgtcagt tctatctaca acctcaaagc agtgctttga gcagcctgcg gctagcttcc
     9961 tagtttggtc tttgattttc attgagtata agaagtcctt ttccttaaag gaagaggctt
    10021 tttacttgct tttttgctgt ttcctcattt gtcctaaagc cttttttgtg ttaaaatgaa
    10081 tggtatgaaa gctaaaaaaa tgtggatggc aggtctggct ctgcttggta ttgggagcct
    10141 tgcccttgct acgaaaaaag ttgcagatga ccataagctc atgaagactc aggaagagtt
    10201 gacagcgatt gtacgagacc atttttcaga catgggggaa attgcgaccc tctatgttca
    10261 agtctatgaa agcagtctag agagcttgct tggtggcgtt atttttgagg atggccgtca
    10321 ttataccttt gtctatgaaa atgaagacct agtctatgag gaggaagtct tatgatacaa
    10381 ccagcaagtt tagaagaatt ggcatcttta gtagaaaaag acggtaagaa ggtcttcctt
    10441 tttgtggcgg actggtgtgg cgattgccgt tatatctatc ctgccttacc agaaattgag
    10501 gagagtaatc cagagtttac ctttattcga gtggatagag accagtatat agatttggcc
    10561 aaactctggg atgtatatgg aatccctagc cttgttgttc tagaaaagga caaggaaatc
    10621 ggtcgttttg tcaatcgcga ccgtaaaagc aagcaacaaa ttaacgattt tttagcagga
    10681 ttgaaatagg agaaaaagga aacaatgatt tttacatata acaaagaaca tgtcggtgat
    10741 gtccttatgg tcatcgtgaa aaatagcgga gatgctaaac tgaatgtgga acgcaaagac
    10801 aaggtagccc gtgttttcct caaagaaaat ggggaaacag tagcttggaa catttttgag
    10861 gtttcgagct tgtttgaaat tgcagagcgc ggtcaagtct ttttatcaga tgagcaagtc
    10921 gctcgtttga accaagaatt acaggcggaa ggttttacag aagaaattgt taatgataag
    10981 gaacctaagt ttgttgttgg tgaaattgtc gagatggtag ctcatccaga cagtgaccac
    11041 ctcaacatct gccaagttgc agtcgcaagt gacaagatag tgcaaatcgt tgcaggagca
    11101 cctaatgcgc gtgttgggtt gaaaaccatt gtggctcttc ctggtgccat gatgccaaaa
    11161 gggaatttca ttttcccagg cgaacttcgt ggtgaaaaga gttttgggat gatgtgtagt
    11221 cctcgtgaat tgcatttgcc aaatgctccg caaaaacgtg gggtgcttga attatcagaa
    11281 gaccaagttg tcggaacacc gttcgaccca gcgaaacact ggactgccta ggaacttgtc
    11341 agcatctgat agaaggaaat aagatggcaa aaaatgtagt gattacggga gcaacctcag
    11401 gaatcggtga agcgattgcg cgtgcttatc tggagcaggg tgaggatgtc gttctaacag
    11461 gacgacggat agacagatta gaaatcctca agtcggagtt tgcagtaagc tttccaaatc
    11521 aaaccgtctg gacttttcca ctagatgtga cggatatggt catggtgaag actgtttgct
    11581 ctgatattct agaaacgata gggaggattg atatcttggt caacaacgcc ggactggctc
    11641 ttgacttggc tccctatcaa gactatgagg agttggatat gttgaccatg ttggatacca
    11701 atgttaaagg tctgatggcg gttactcgct gtttcttgcc agcaatgata aaagtcaatc
    11761 aaggtcacgt tatcaatatg gggtcaaccg caggaatcta cgcctatgct ggtgccgctg
    11821 tttactcagc taccaaggca gcggttaaga ccttttcgga tggactgcga attgatacca
    11881 tcgcaacgga tatcaaggtg acaaccattc agcctgggat tgtcgaaaca gatttctcaa
    11941 ctgttcgttt tcatggtgat aaagagcggg ctgcgtccgt ttaccaagga atagaagcct
    12001 tgcaagctca ggatattgca gacatagtag tctatgtgac cagtcagcct cgccgtgttc
    12061 agattacaga tatgaccatt atggccaatc aacaggcgac aggtttcatg attcataaaa
    12121 aataagaaat ttcctcgaaa agttacaaat ttctgtaact ttttttgatt tcctacgaat
    12181 agataagtag gaggaagaaa atatgtataa taaagttatc atgattgggc gtttaacgtc
    12241 tacaccagaa ttgcacaaaa ccaacaatga caagtcagta gcgcgagcaa ctatcgctgt
    12301 caaccgtcgt tacaaagacc aaaacggtga acgtgaagct gattttgtca atatggttct
    12361 atggggcaga ctagcagaaa ctttggcaag ctacgcaacc aaaggtagtc tcatttccgt
    12421 tgatggagaa ttgcgtaccc gtcgctttga gaaaaatggt cagatgaatt atgtaaccga
    12481 agtccttgtg acaggattcc aactcttgga aagtcgtgcc caacgtgcta tgcgtgaaaa
    12541 taatgcaggc caagatttgg cagatttggt cttggaagag gaagaattgc cattttaaga
    12601 attaaaaagt ctgagttggt ctcaggcttt ttatcttgag aaagccagac ttttttcttg
    12661 actatttctg accaagtgat acaatagaat tatgaattag cactccagtt caaagagtgc
    12721 taataatatc tatctcatta tggaggaaat cagatgttga aaccattagg ggaccgtgtg
    12781 ctcttaaaaa tagaagaaaa agaacaaacc gttgggggct ttgtccttgc aggctcagcc
    12841 caagaaaaaa ccaaaacagc tcaagttgtg gctactggac aaggtgttcg taccttgaac
    12901 ggtgacttgg ttgctccaag tgttaaaact ggagatcgtg tcttagttga agcccacgca
    12961 ggtcttgatg tcaaagatgg cgatgaaaag tacatcatcg taggcgaagc taacattttg
    13021 gcaatcattg aggaatagaa ggagaaagta agtatgtcaa aagaaattaa attttcatca
    13081 gatgcccgtt cagccatggt tcgtggtgtc gatattcttg cagacactgt taaagtaacc
    13141 ttgggaccaa aaggtcgcaa tgtcgttctt gaaaagtcat tcggttcacc cttgattacc
    13201 aatgacggtg tgaccattgc caaagaaatc gaattggaag accattttga aaatatgggt
    13261 gctaagttag tatcagaagt agcttctaaa accaatgata tcgcaggtga cggaactacg
    13321 actgcaacag tcttgaccca agctatcgtc cgtgaaggaa tcaaaaacgt cacagcaggt
    13381 gcaaatccaa tcggtattcg tcgtgggatt gaaacagcag ttgccgcagc agttgaagct
    13441 ttgaaaaaca acgccatccc tgttgccaat aaagaagcta tcgctcaagt tgcagcagta
    13501 tcttctcgtt ctgaaaaagt tggtgagtac atctctgaag caatggaaaa agttggcaaa
    13561 gacggtgtca tcaccatcga agagtcacgt ggtatggaaa cagagcttga agtcgtagaa
    13621 ggaatgcagt ttgaccgtgg ttacctttca cagtacatgg tgacagatag cgaaaaaatg
    13681 gtggctgacc ttgaaaatcc gtacattttg attacagaca agaaaatttc caatatccaa
    13741 gaaatcttgc cacttttgga aagcattctc caaagcaatc gtccactctt gattattgcg
    13801 gatgatgtgg atggtgaggc tcttccaact cttgttttga acaagattcg tggaaccttc
    13861 aacgtagtag cagtcaaggc acctggtttt ggtgaccgtc gcaaagccat gcttgaagat
    13921 atcgccatct taacaggcgg aacagttatc acagaagacc ttggtcttga gttgaaagat
    13981 gcgacaattg aagctcttgg tcaagcagcg agagtgaccg tggacaaaga tagcacggtt
    14041 attgtagaag gtgcaggaaa tcctgaagcg atttctcacc gtgttgcggt tatcaagtct
    14101 caaatcgaaa ctacaacttc tgaatttgac cgtgaaaaat tgcaagaacg cttggccaaa
    14161 ttgtcaggtg gtgtagcggt tattaaggtc ggagccgcaa ctgaaactga gttgaaagaa
    14221 atgaaactcc gcattgaaga tgccctcaac gctactcgtg cagctgttga agaaggtatt
    14281 gttgcaggtg gtggaacagc tcttgccaat gtgattccag ctgttgctac cttggaattg
    14341 acaggagatg aagcaacagg acgtaatatt gttctccgtg ctttggaaga acccgttcgt
    14401 caaattgctc acaatgcagg atttgaagga tctatcgtta tcgatcgttt gaaaaatgct
    14461 gagcttggta taggattcaa cgcagcaact ggcgagtggg ttaacatgat tgatcaaggt
    14521 atcattgatc cagttaaagt gagtcgttca gccctacaaa atgcagcatc tgtagccagc
    14581 ttgattttga caacagaagc agtcgtagcc aataaaccag aaccagtagc cccagctcca
    14641 gcaatggatc caagtatgat gggcgggatg atgtaagctt tctatagaaa acaacttata
    14701 aaaaacacaa aaggagggaa tgactaaccc ttctttttat aatattcact tctaaattga
    14761 tttgagctct cctaacttat atgataaaat aagactagaa aaaggagaag aacatgatcg
    14821 atgtagaaga aattctgtgc aagatgaccc ccaatcagaa gattaattat gaccgtgtca
    14881 tgcagaaaat ggtacaagca tgggaaaaaa atgagtagcg gccaaccatt ctcgtgcatg
    14941 tttgctgtgc cccttgtagt acctatacac tagaatattt gaccaagtat gcagatgtga
    15001 ccatctattt tgccaattct aatatccatc ccaaggcaga ataccataag cgggtctatg
    15061 tcaccaagaa atttgttagt gattttaatg agcggacagg aaatacggtt cagtacctag
    15121 aagctcccta cgaacccaat taataccgaa aactagttag ggggctagag gaggagcccg
    15181 aaggtggcga ccgttgcaag gtttgttttg actaccgact ggataaaaca gcgcaagtgg
    15241 ctatggactt gggctttgac tactttggtt cagccttgac catcagtcct cataagaatt
    15301 ctcaaactat caatagcatc ggaatcgatg tgcaaaaaat ttacacgccc cactattttc
    15361 ccaacgattt caagaaaaat caaggctaca aacgttcagt agagatgtgt gaggagtatg
    15421 atatctatcg tcaatgttat tgtggctgcg tctatgcagc ccaagcccag aatattgacc
    15481 tggtttaagt taagaaggac gccacagcat gcttgttgga taaggatgtt gagaaagact
    15541 attctcatat caaatttata gtagattgaa actagaatag tacaccttta cttctaaaac
    15601 attgttagaa atcgatttga ctgtccttat ttcattttac tatactgtta cgaaattaga
    15661 tatataaatg aaaacttgcc tcaaggtagg ttttttgcta gtagaaaagc gatagacaga
    15721 aattgttgga ctcttaaaaa aatgaagttt tgctcaaaag agctttttaa attaaaacga
    15781 gcaattatca gttcaaatct gtttcaaaag aagaacggat aaattaatcg atgctttgtt
    15841 tttgagttag gaagttaagc atgccaaact gagtcgtagg aataaatttc tttatagaaa
    15901 tgtgttacaa ctctgctata acggaatatt ccttttttgc a
//
LOCUS       DAVMAF010000039        15164 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMAF010000039 DAVMAF010000000
VERSION     DAVMAF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.039"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(104..454)
                     /locus_tag="VYO97_001303"
     CDS             complement(104..454)
                     /locus_tag="VYO97_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754091.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDCICFKKMLDLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(594..929)
                     /locus_tag="VYO97_001304"
     CDS             complement(594..929)
                     /locus_tag="VYO97_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754092.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWIYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1020..2330)
                     /gene="der"
                     /locus_tag="VYO97_001305"
     CDS             complement(1020..2330)
                     /gene="der"
                     /locus_tag="VYO97_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1754093.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPGMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2344..3057)
                     /locus_tag="VYO97_001306"
     CDS             complement(2344..3057)
                     /locus_tag="VYO97_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1754094.1"
                     /translation="MTETIKLMKAHTSVRRFKEQVLPQEDLTEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLKQKKLL"
     gene            complement(3054..3950)
                     /gene="dnaI"
                     /locus_tag="VYO97_001307"
     CDS             complement(3054..3950)
                     /gene="dnaI"
                     /locus_tag="VYO97_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1754095.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTTWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3951..5120)
                     /locus_tag="VYO97_001308"
     CDS             complement(3951..5120)
                     /locus_tag="VYO97_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1754096.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEYLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEAIIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(5121..5594)
                     /gene="nrdR"
                     /locus_tag="VYO97_001309"
     CDS             complement(5121..5594)
                     /gene="nrdR"
                     /locus_tag="VYO97_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1754097.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5734..6099
                     /locus_tag="VYO97_001310"
     CDS             5734..6099
                     /locus_tag="VYO97_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1754098.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6104..6799
                     /locus_tag="VYO97_001311"
     CDS             6104..6799
                     /locus_tag="VYO97_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754099.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6816..7583
                     /locus_tag="VYO97_001312"
     CDS             6816..7583
                     /locus_tag="VYO97_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754100.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7936..9135)
                     /locus_tag="VYO97_001313"
     CDS             complement(7936..9135)
                     /locus_tag="VYO97_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754101.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9132..10025)
                     /locus_tag="VYO97_001314"
     CDS             complement(9132..10025)
                     /locus_tag="VYO97_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754102.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10142..10222
                     /locus_tag="VYO97_001315"
                     /pseudo
     CDS             10142..10222
                     /locus_tag="VYO97_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10707..10920
                     /locus_tag="VYO97_001316"
                     /pseudo
     CDS             10707..10920
                     /locus_tag="VYO97_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11335..11952
                     /locus_tag="VYO97_001317"
     CDS             11335..11952
                     /locus_tag="VYO97_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1754103.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(11995..12882)
                     /locus_tag="VYO97_001318"
     CDS             complement(11995..12882)
                     /locus_tag="VYO97_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1754104.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIEFNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(13031..14914)
                     /locus_tag="VYO97_001319"
     CDS             complement(13031..14914)
                     /locus_tag="VYO97_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1754105.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
BASE COUNT         4471 a         3313 c         2789 g         4591 t
ORIGIN      
        1 ttcaatccac tatacaagat gaatatttct ctcaagtttc ataatcaaaa taaaaatgac
       61 tgtatctata gctccataaa caaatcatag atacagtcat tttttagttc ctacaccttc
      121 ctttatgcac acctttagac ttccctagtt cccgtctcaa gtcaagcatt tttttgaaac
      181 agatacagtc taaccagtca ctagcaaaag cgataagtag taataatagc caaatacccc
      241 ataacttgat atctgtcaaa tttctcaaga cgatattgaa aaacagaact gaaaaaactg
      301 tccaagcaag gctaaaaaga gcatagaggg tgatgtaaat ccagtaaaaa tagtaaaaaa
      361 ttgggaaaaa tttactttct ctattatcct tgtcaatcca gctgaaaaag taaaaacagg
      421 gtaataagat tatcatttta aacaagtgtt ccatcacaag cctccttttt taaattgttt
      481 tctttagttt cgttctatta aatgttatta ccaatataaa ccttggaata gcgtgtaata
      541 aatgatttta gaggtaccat aagccacctc ctacaaatag aaaccgatat aaatcaatgc
      601 cttccaccct tagacttccc tagttcctgt ctcaagcgaa acatttcttt gaaacaggaa
      661 taagttaacc aattcatacc aatagctagc agaataaaaa gaaaccaaat gccccataac
      721 ttgatatctg tcacatttct caagacggta ttgaaaaaca gaactgaaac aactgtccaa
      781 gcaaggctaa aaagagaata gaaggggatg taaatccagt aaaaataata aaaaattgga
      841 aaaaacttac tatttctgtt ggccttttca atccagttat caaaataaaa gtacggtgct
      901 aaaagtaaga atttaaacaa atgttccatc accgacatcc ccctccttct tttaatagcg
      961 ttttctatta ttttattata tcaaaagaat ctggaaatgt cattccagat tctacttttt
     1021 tatttacgct ttcttgcgat gagatgaatc ggtgttccct caaagacaaa ggccttgcgg
     1081 atttgatttt ccaagaaacg taggtaagaa aagtgcatga gttcttcttc gttgacaaag
     1141 ataacaaagg ttggtggttt ggttgccact tgggtcgcat agaaaatctt gagacgtttt
     1201 cctttgtctg tcggtgttgg gttgatggca atggcatcca tgatgacatc gttcaagaca
     1261 gctgatggaa tacgtgtatt ttgactttcg ctgatttgct taatcatccc aggaagtttg
     1321 tggagacgtt gcttggttaa agctgataca aagataatcg gtgcgtaagg caggtattgg
     1381 aactgctcac ggatatcttc ttcccagttt ttcatagtgt ggttatcttt ttcaagcgta
     1441 tcccacttgt tgaccacgat aatcatccct ttaccagctt catgagcaaa tccagcgata
     1501 cgcttgtcgt attcacgaat cccctcttcc gcattgatga ccattaagac cacatctgaa
     1561 cggtcaatag cacgcatggc acgcatgaca gagtacttct cagtattttc ataaacctta
     1621 ccagacttac gcataccagc cgtatcaatc atggtaaact cttgaccatc tgtatctgta
     1681 aagtgggtat caatagcgtc acgagttgtt ccagcaacag gactggcaat aacacggtct
     1741 tcgcccaaga tagcattgat caagcttgat tttccaacgt ttggacgacc aatcaggcta
     1801 aacttaatga catctggatt ttcttcctca tattcatttg gaagattttc tacgatagca
     1861 tctagtacat cccctgtacc gattccatgg acagatgaga taggcagtgg ttcacccaaa
     1921 ccgagagcat agaaatcata gatatcattt ctcatctcag ggttgtccac cttgttgact
     1981 gcgaggataa ctggtttgtg ggtcttataa agcttacgag ctacgtattc gtctgcatca
     2041 gtaattcctt ccttaccaga cacgacaaaa acgataacat ctgcttcttc catggcaatt
     2101 tctgcctggt gcttgatttg ttccatgaaa ggagcatcta catcgtcgat tcctcctgta
     2161 tcaatcatac taaaagagcg attgagccac tcacccgttg catagatacg gtcacgtgtc
     2221 actccttcga catcttctac aatggagatt cgctcaccag cgatccgatt aaatagggtt
     2281 gatttcccaa cattgggacg tcctacaatg gcaatagttg gtagggccat aatttctcac
     2341 tttctacaat aacttcttct gtttaagatt ttttctagtt gagcttggtt ctgcttggcc
     2401 aaactgttct gctagacgct gactccagct tgtggtcgca cgcgctccag catagtcagc
     2461 ctgcacacgg tcataagctt ggattgcctc agttgactgt tcttggtatt cttcctcaaa
     2521 gactacatta tctagtggca gtctcggttt cacatcatga gtttgatttg gcacacccag
     2581 tgccatccca aagacagaat aggtgtagtc aggtaggtta aagagctctg ccacttcttc
     2641 agacttgtat cgaaccaaac cgataatcac accaccatag cccaagcttt cagctgccaa
     2701 caaggcattt tgtccagcaa gagctgcatc gaccgaacta atcaagagac cttccacacc
     2761 ttggggttgg aaggtgtcgg tatgaagtcg ggctcccttt tctgctcggt tcaaatctcc
     2821 gacaaagaga aggaaaacag cagactggcg aatggcttct tgaggtacca attcatacaa
     2881 ggcatctttc ttctcttggc ttcgtaccac aatcacagag taggattgga aattcttcca
     2941 agatgatgcc atctgggctg ctgtcaggat ttcagtcaaa tcttcctgag gaaggacttg
     3001 ctccttaaat ctgcgcactg aagtatgagc cttcatcaat ttaatggttt ctgtcatcga
     3061 cggtttactc cttctaaacg agtctcctca gccaaataac ggatgcgttc catgacgcgt
     3121 ctggcttccc aggtttcgtc atttccattt ttccctttag cgaaatgctt ctccaaatct
     3181 tcaaagttga agttagaggt gaaaaaggtc ggtaaatttt cctgcatccg atattggaga
     3241 atgacctgca ggatttcatc acgcacccaa gtggtggatt gctcggcacc aatatcatct
     3301 aaaatcagga cttcagaaag cttaatctca tccactaagg tcttaacatt gccatcactg
     3361 atagcatttt tgacatcaat gacaaagcta ggatagtgga ggagagtgga tgatacacca
     3421 cgtttttctg ataaatcatg agctagggca gccaccatga aacttttacc cacaccaaag
     3481 tctccataca agtaaagacc tttcaaatta ttggggtaat cgttaacaaa ttctatcatt
     3541 ctttttagaa cagttaagcg ctgaacatca tcacgataaa catctaaaaa actgacattt
     3601 ttcaggctag atggaaaatt aattaagttg agacgtttct tgatagccgc ttctttttca
     3661 gctgcgatta gttcaggagt ttcttcataa gaaacatccg catagccatg gttcatgact
     3721 aatataggct tgtagccttt ggcaatataa tctgtatctc ctcggaggaa tttatctctc
     3781 tcagtgatgt attggttaaa cttggaaata ctacggttca actcgtcctg attgagggat
     3841 tcttgctgga caaaggccgc aacatcagga tccttcatga ttttctgaac taaatcttga
     3901 tagtgaaaac gacttggttg acgtttgagt acatctccga cactttccat ctaatcacct
     3961 ccgttatcta attttgctaa taattctagg gtttggcgtt ccatctcttc taattcctca
     4021 gcggatgacg tatttttata ttcaggatta ctccatttag gaatattggt ttttgctggg
     4081 gcagtctgat tctgtttttg tgtttttgct ttctgtcctc gctcacggat gcgcaagact
     4141 gcctcttctg ccgaatgaat cttttgatag gcatagtcat tggctacctt catggcatat
     4201 ttctcattga tatttgccga atcgacctta ttaaaggtca ataaaagaat gatattgatg
     4261 acttcgtcca gcaaacccaa gccagccatc tgttgcaaga gttctctttc tgtttgggta
     4321 atggttccct tgcgtgtttg cttgatttct gccaagaact gcagggcagt tttacttttg
     4381 gcttctttga taatgatcgc ttccttaaga ctaaagtcag aggaaactgg tttttgagca
     4441 attttttcac gcatgcgttt ggttgaaata acctgagaaa cagctgttga cttggccaat
     4501 tgataggttt caaaccaagt ccatttcttc tcctcggcaa tggcaaagag gtttaagaca
     4561 tcggactgct catccgcaaa gcgaagtcca tctcgagcca taagctgacg aaaatggtcc
     4621 aagtcaaaat cgttggccac tttcttcttg agaccaaggt attcttgact gcctagttct
     4681 gccaattctg gaaagacttg attgagtgag acaggtattt cttcaccatc agcactttca
     4741 actttcaaat cctccacagc tacatcgcca atctttttct ctaagagtct gcgataaaca
     4801 ggatgcccca agaagtcttg actagataga ggagcatgga gggctagctg ataaacatcc
     4861 cccttttgat agagagtcaa gagattaaaa gcagataaga ttttcaatga ttttatcagt
     4921 ctatccatcc caaagttgag atgattgagg atacttgaaa aaagatattc ctttctacca
     4981 ttatcccaaa aactaatcgt ataaagataa aggctcagtg cctcctgacc gataatcggg
     5041 aggtagcact gtaccagaga tgaggtatct tgcgacaccc gattattctt tagataagaa
     5101 aaacggtcaa ttggcttcat ttatctttcc tttttctttt tagaggactg ggtgatttgt
     5161 tggagcaagc tctctaactc actgacatcc ttaaaactac gatagacact agcaaaacgt
     5221 acataggtaa tctcgtccaa ttcagccaac tcctccatga cgagtgaacc aatgtcctca
     5281 ctttgaattt cattttcatt tcgaccacgg agtttctgtt cgatacgatt gactaccatg
     5341 ttgatttcat cacttgacac aggacgtttc tgggctgagc ggataatccc attaaagatt
     5401 ttatctctgg agaattgttc ccgtgtgcca tcttttttaa caaccactaa ggttctttct
     5461 tctactcgtt cgtaggttgt aaaacggtgt tggcactcgt cgcactctcg tcttctacga
     5521 atggtgttcc cttcttctgc ttggcgacta tcgataacac ttgacttggt agccccacat
     5581 tttggacaac gcatcctttc cctccttatc gttttctttt cattatacca ttttttaagc
     5641 gattcccaaa acaattcttc tttttgcttg acaagttttt tgttttgttg tattatttaa
     5701 ttaagacaac aaggtaaaag aaaggagact aagatgtcct ggacatttga caacaaaaaa
     5761 cccatctatt tacagattat ggagaaaatc aagcttcaga ttgtttccca tacactggaa
     5821 cccaatcaac aacttccaac cgtgagggag ctagctagcg aggctggtgt caatcccaat
     5881 accatccaaa gagccttatc agaccttgaa cgagaaggat ttgtctacag caagcgaaca
     5941 actggacgat ttgtgactaa ggataaggag ctaatcgccc agtcacgcaa acaattatca
     6001 gaagaagaat tggaacactt cgtttcctcc atgacccatt ttggctatga aaaagaagaa
     6061 ctaccaggcg tagtcagtga ttatattaaa ggagtttaag cctatgtcat tactagcatt
     6121 tgaaaatgta tccaaatcat atggagcaac accagccctt gaaaatgttt ctcttgacat
     6181 tccagctgga aaaattgtcg gccttcttgg gccaaacggc tcaggaaaaa caaccctgat
     6241 taaactaatt aatggcctct tacaaccaga tcaaggacgt gtcctcatca acgacatgga
     6301 cccaagccca gcaaccaagg ccgttgtagc ttatttgcct gatacgacct atctcaatga
     6361 gcaaatgaag gtcaaagaag ccctaaccta cttcaagacc ttctataaag atttcaatct
     6421 tgaacgcgcc catcatctac ttgcagacct gggcattgat gaaaatagtc gtctcaagaa
     6481 actatcaaaa ggaaacaaag aaaaggttca actgattttg gttatgagcc gtgatgctcg
     6541 tctctatgtt ttggatgaac ccattggtgg ggtggatcca gcagcccgtg cttatatcct
     6601 caataccatt atcaacaact actcaccaac ttctaccgtt ttgatttcta cccacttgat
     6661 ttctgatatc gagccaatct tggatgaaat tgtcttccta aaagacggaa aagtcgtccg
     6721 tcaaggaaat gtagatgata ttcgctacga gtcaggtgaa tccattgacc aactcttccg
     6781 tcaggaattt aaggcctaag caaaggagat tatttatgtt ttggaattta gttcactacg
     6841 aatttaaaaa tgttaacaag tggtatttag ccctctacgc agccgtgcta gtcctttctg
     6901 ccctcatcgg aatacagaca caaggcttta aaaatctacc ttaccaagaa agtcaggcta
     6961 ctatgctact ttttctagct acagtctttg gtggcttgat gcttacactt gcgatttcaa
     7021 ccattttctt gattattaaa cgcttcaaag gtagtgtcta cgaccgacaa ggctatctga
     7081 ctttgacctt gccagtttct gaacaccata tcatcacagc caaactaatc ggtgccttta
     7141 tctggtcatt gattagcacc gctgtattgg ctctaagtgc tgttattatt ctggctttaa
     7201 cagctccaga atggattcct ctttcttatg tgattacatt tgtagaaaca catctccctc
     7261 agatctttct tacaggtata tccttcctac taaataccat ttcaggaatc ctctgcatct
     7321 acctggctat ttccattgga cagcttttca atgaataccg tacagcactc gctgttgcag
     7381 cctacattgg tatccaaatc gtcattggat ttattgaact tttcttcaat cttagttcta
     7441 atttctatgt caattcactg gtaggactca atgaccattt ctatatggga gcaggtatag
     7501 ccattgttaa agaactcata ttcatagcta tcttttatct cggaacctac tacatcttga
     7561 gaaataaggt taatttgctt taaataattt ttacctagat atgtaacata ctcatagaac
     7621 aaaagagacc aggcaaaaag tttttaaaat tagaaaacgc atagtatcaa gtgttaaaaa
     7681 tccttgatat tatgcgtttt ttgtgagctg acttatttcc tttcactata tcgcaaaatg
     7741 aaataagaac ggaacgatgg gattttggaa ttcaaatcaa tttataagaa tgttttagaa
     7801 gtaatattat cctattccag attcagttca ctatacaatt gagttttcaa gcaacctgtt
     7861 tacataatgt gtacataatt aggttcgtga ttccaccctt ttcaccttta aaaacctcgc
     7921 tttcgcaagg ctcttctatt tataagataa ggcacgttta aaggttttcc aaatccctaa
     7981 atcatccgtt tgaagaacga gactagcata catgcgtccg ataaatcctg ttgctaccac
     8041 cgcaaaaatc actgtaatag caagtgaaat ccatgcttct gctccccccg catagccatt
     8101 aatcgttcga aacggcataa agaaggtcga aataaaggga atataagaac caatcttcaa
     8161 gaggagattg tcaccagctg cacctagagc tgtcactcca aaaaaaccac ccataatcaa
     8221 aatcatcaaa ggcgacaagg ctttccctga gtcctcagga cgagaaacca tagatcctag
     8281 gaaggctgcc aagactacat acatgaaaag actgatcaaa ataaagagca aggtattcag
     8341 tgagatagca tctcccaagt gatccaaaat accagactga gccaagaatg gcaaatcttt
     8401 aaagagcaaa acggcagcca gaccacctac aacatagatc ccaatatgcg ttaaaatcac
     8461 tagaaacaga gccatcatcc gcgcatagaa atagtgactt gcccttatgc tagaaaaaac
     8521 gacttccata attttggtgc ctttttcact ggcaacttcc tgagctgtta cacccgcata
     8581 ggtaatcaga atcatataaa gaaagaatcc taaggcacct gctgcaattg tttgaataaa
     8641 ctttttattt tccttggctt catcaatctt ttctgtgaat tgaattgtct gcgctaagcg
     8701 tttttcctgc tcttgagaca aggaagcagt tgaacgatta agctgatttt gcagttcatt
     8761 gagtgtacct gtaacctcaa atttaattcc attttcaagc gatgtttcgc catgataaac
     8821 tgcctttaga acactatctt cttgatcaat ggtcaaataa ccttttaatt tttcttcttt
     8881 aattgcttct ttggcacttg cttcgtcttt atagtcgaag ttaacaccat ttacattctt
     8941 cagtccttct gctacagatg gcactgttgt cactactgcc actttattat ttttagccat
     9001 agaagaacct tggatatgcc caattcctac agagattcct aaaaagagga acggcgaaat
     9061 caccataaag aagaaactcc atgacttgac atgtcgaaga taggtttcct tgattacaac
     9121 ccacatattt ctcatacttc cactcctgat tctagtttaa agatttcatc gatagttggc
     9181 gcttgttggt caaatgttgc gatatattga ccttgagtca agattgggaa gagttccctt
     9241 ccagcgctct catcctccaa aatcaatttc caactgcctt gtttggtcaa gctcacctgt
     9301 ttgacatgag gaagattttc caattcttcc ttgcttcgtt cacttgaaac aaagagacgc
     9361 gttttcccgt attgattgcg gacatcctga actggtccgt gcaagaccac acggccatct
     9421 cggatcatca gaatatcgtc acaaagttcc tcaacattgg tcatgacatg gtcagaaaag
     9481 ataatggttg ctccgcgctc tttttcctga aaaatgactt gtttgagcaa ttctgtatta
     9541 actgggtcca atccactaaa aggctcatcc aagataatca ggtctggttc atgaatcaga
     9601 gtaataatga gctgaatctt ctgctgattt ccttttgaca gactcttgat tttatctgtc
     9661 agctttcctt tcacttccaa cctcttcatc cattgaggga gtttttcttt gacttctttg
     9721 gcatccatgc cttttagagt cgccaagtag cgaacttgtt caagaactgt caatttaggc
     9781 atgagactgc gttcttcagg cagataacca atccgagcat aggtctcctg acgaatatcc
     9841 tgaccatcca gaccgatttc tccctgatat tctaggaatt tcaaaatact atggaaaatc
     9901 gttgtttttc cagcaccatt ttttccgact agtcccaaaa tacgacctgg tcgcgcttga
     9961 aagtcaatac caaacaaaac ttgcttggat ccaaaacttt tctctagact tcttacttct
    10021 agcatctttc acctccgaaa tttcttgcac tcattatact cctttttgat agcctttaca
    10081 atgttttttg tccattttta gaagactatt gctgtgtaaa atatggcctg gagcactttt
    10141 atactcaatg aaaagcaaag agcaaactag gaagctagcc gtaggctgct caaagtacag
    10201 ctttgaggtt gcagataaaa ctgacgaagt cagctcaaaa cactgttttg aggttgtgga
    10261 tagaactgac gaagtcagta actatatcta cggcaaggcg aagctgacgt ggtttgaaga
    10321 gattttcgaa gagtattagt gataaatcca ttatacagta gcaaacttaa tttatacctt
    10381 ccgctcctca actgtctatt tttaatcctg aattgttatt tgagtaactc ctttttcctc
    10441 ataaagtttt cttcctctaa aacttctgga aaaaagcgaa tagtttcaga caacattttt
    10501 ataagaaaca agttcatctg tcatttcaag aaggagtaat cctttatcta ctaatggacg
    10561 gaacggaatt caaccgcttg tccgatatgt tttctaagga ttatatagta aaatgaaata
    10621 aaaacatgac aaatagatcg ggacagtcaa atcgatttct aacaatgttt tagaagtaga
    10681 tgcgtactat tctagtttca atctattata tctattatgc acaccactat aggatctaat
    10741 aaaaatcaca acaagttcat tcatagatgg ttacctcagg gaactaagaa aacgactaca
    10801 aaggaagtcg cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttgaactac
    10861 aagtccccca gagaagactt ctggatgact aacttgaact tgaaatttag caataattaa
    10921 ttcaccatct aactatattt agtaattatt tcagaactga ttaatattaa aattaactaa
    10981 caattcaaag gattcatact agccataaat tacgtccatc agagagagac tcttactact
    11041 tttagatttt agtctttcta gcttcagaat acatctaaac tttagggaaa atgactattt
    11101 gaaagcgcga atgcctcaaa attatctcag ataagctatt cgaaacttag aatgctttta
    11161 aatttatgga attgcgatta ttcgaaacct agaatgcata taacctttag ttgacagacc
    11221 tattctaagt ctcgaagggc tatttacttt ctattcctta tcaaaaaaga ctcattcccc
    11281 ctttctcctc caaaatatgg tatagtagaa atatactatc tatgaggagt ttacatgtca
    11341 caggataaac aaatgaaagc tgtttctccc cttctgcagc gagttatcaa tatctcatcg
    11401 attgtcggtg gggttgggag tttgattttc tgtatttggg cttatcaggc tgggatttta
    11461 caatccaagg aaaccctctc tgcctttatc cagcaggcag gcatctgggg tccacctctc
    11521 tttatctttt tacagatttt acagactgtc gtccctatca ttccaggggc cttgacctcg
    11581 gtggctgggg tctttatcta cgggcacatc atcgggacta tctacaacta tatcggcatc
    11641 gtgattggct gtgccattat cttttatcta gtgcgcctat acggagctgc ctttgtccag
    11701 tctgtcgtca gcaagcgcac ctacgacaag tacatcggct ggctagataa gggcaatcgt
    11761 tttgaccgct tctttatttt tatgatgatt tggcccatta gcccagctga ctttctctgt
    11821 atgctggctg ccctgaccaa gatgagtttc aagcgctaca tgaccatcat cattctgacc
    11881 aaacccttta ccctcgtggt ttatacctac ggtctgacct atattattga ctttttctgg
    11941 caaatgcttt gacacgtaaa aaatccgttt ggtttcccaa gcggattttg tgctttattt
    12001 tgaaacttct tttgcaagaa caaagttccc aagtgtggca gaaccatttc ctgcgactgc
    12061 tggcgtcacg atatagtcac gcacatctgg tactggtagg taaccattaa gaagagatgt
    12121 aaatttctca cggacacggt ccagcatatg ttgttgagcc atgacccctc caccaaagac
    12181 aatcacgtct gggcggaaag tcactgtcgc attaaccgca gcttgagcga tatagtaggc
    12241 ttgaacatcc caaacagggt tgttgaattc aatagtttcc ccacgtacac ctgtacgagc
    12301 ttccaaactt ggaccagctg cataaccttc tagacatccc ttatggaaag gacaaacacc
    12361 cttaaactct ttttcaatat ccattgggtg tctagcaaca taataatgac ccatttcagg
    12421 gtgacccaca ccaccgataa actcaccacg ttggatgacg cctgcaccga tacctgtacc
    12481 gattgtgtag taaaccaagt tttcgatacg accaccagca ttgttacggg caaccatttc
    12541 accgtaagca gagctgttta cgtctgttgt gaagtacatt ggcacgttta gggcgcgacg
    12601 aagggcacca agcaagtcca catttgccca gtttggtttt ggagtcgtcg tgataaagcc
    12661 ataagttttt gagtttttgt caatatcaat cggcccaaat gaaccaactg caagaccagc
    12721 aaggttatcg aattttgaga agaactcaat ggttttatcg attgtttcga ttggagttgt
    12781 tgttggaaat tgtgtttttt ctacaacgtt aaagttttca tcaccgacag cacagacaaa
    12841 ctttgtaccg cccgcttcca agcttccata taattttgtc atgataaacc tcttgttttt
    12901 attttcttta ttatagcata cttcgaaagt ctaaatgtct ctatttttta gattttcctc
    12961 tgtaaatctt actatctaat aaaaacgaac aaacatgtca tttgttcgtt ttcacattag
    13021 agaggattga ttagattttc acttcgatca cagcatcccc cttcgcaact gaacctgttg
    13081 cgactggagc tactgaagcg tagtcagctg tatttgtaac gataaccatt gttgtatcat
    13141 caagtccagc tgcagcgatt ttgtttgagt caaatgttcc aagaacatcg ccagctttca
    13201 ccttattacc ttgagcaact tttgcttcaa aaccgtcacc gttcatagat acagtgtcaa
    13261 taccaacgtg aatcaaaact tcagcaccat ttcttgtttt caaaccaaaa gcgtgccctg
    13321 ttggaaaggc aattgaaact tcagcatcag ctggtgcata gaccacacct tggcttggtt
    13381 tcacagcgat accttgtccc atagctccac ttgagaagac tgggtcattg acatcagcaa
    13441 gagcgacaac atcaccaacg ataggagtta caagtgtttc attttgaaga gctgctggcg
    13501 caacttcttc tttttcttca gccacttcag cttgttttgc agctgcagtt gcgtctactt
    13561 catcttcgta accaaacatg taagtaagag caaaaccaag ggcaaatgat acagctacca
    13621 taagaaggta ttgtggaagt tgtccgttac caacataaag cattgtacca gggatgatgg
    13681 tgataccatt accagtacca gcaagtccaa ggatagaagc caatccacca ccgattgcac
    13741 cagcaatcaa tgaaaggaag aatggtttac ggaagcgcaa gttcaccccg aagatagcag
    13801 gctctgtaat acctaggaag gcagaaagag cagccgggaa agcaagtgtt ttcagttttg
    13861 gattttttgt tttaacacca accgcaacag tagcagcacc ttgagctgtc atagcagctg
    13921 tgatgatagc gttgaatggg ttagcatggt cagcagcaag taattgcact tcaagcaagt
    13981 tgaagatgtg gtgcacacct gacacgacga tcaattggtg aaccccacca atcaagaaac
    14041 caccaagacc aaatggaatg ctaagaatcg cttttgtagc aataaggatg tagttttcaa
    14101 caacgtggaa aactggtcca atgacaaaga gtccaaggat agacatgacc aaaagtgtca
    14161 cgaatggtgt taccaagagg tcaatgacat ctggaacaac cttgcggaca gctttttcaa
    14221 atttagctcc gacaaccccg atgatgaagg ctggaagaac ggaaccttgc aaaccaacaa
    14281 cagggatgaa accaaagaag ttcatcgctg ttacttcacc accttgagca actgcccaag
    14341 cgtttggaag tgagccagag acaagcatca taccaagaac gataccaacg gcaggatttc
    14401 caccaaatac acggaaggtt gaccacacaa ccaaacctgg caagatgatg aaggctgtat
    14461 ctgtcaagat ttgtgtgtaa gttgcaaagt cacctggaag tggcatttca agagcgttga
    14521 aaagaccacg cacacccatg aagagacctg tcgctacgat aactgggatg attggaacga
    14581 aaacatcacc gaaagtacgg atagcacgtt ggaaccagtt cccttgttta gcaacttctg
    14641 ctttcatgtc atccttagat gatgttggta agccaagtac aacaacttca tcgtacattt
    14701 tgttaactgt acctgtacca aagataattt ggtattgccc tgagttaaag aaagcacctt
    14761 gaactttttc caagttctca atcacttctt tattgatttt ctcttcatct ttgaccatga
    14821 cacgtagacg agtcgcacag tgggcaacac tattgacatt ttcacgtccg cccaaggcat
    14881 cgatgacttt ttttgcaatt tcctgattgt tcatttgcaa aaatctcctt atataacatt
    14941 ttgttcttgt ttgaaagcga ttttattcgc ccttacgact attattttat catgttttaa
    15001 aaatatgtca agcgttttgc agaaaaaata ttctatccac ttagtgggca agttttagat
    15061 tgattgtttt tgactgtttc atcaggaatt ccttgaaaaa taagtttgaa accttggtta
    15121 aaaagtattt tatttcatta tctttcacat tttcgtaaaa attt
//
LOCUS       DAVMAF010000040        15088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMAF010000040 DAVMAF010000000
VERSION     DAVMAF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.040"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            14..490
                     /locus_tag="VYO97_001320"
     CDS             14..490
                     /locus_tag="VYO97_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1754106.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            528..830
                     /locus_tag="VYO97_001321"
     CDS             528..830
                     /locus_tag="VYO97_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1754107.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            838..1642
                     /locus_tag="VYO97_001322"
     CDS             join(838..1206,1208..1642)
                     /locus_tag="VYO97_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1754108.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKQLANYSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQIPRKSSKLKPLIAEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            1691..2155
                     /locus_tag="VYO97_001323"
     CDS             1691..2155
                     /locus_tag="VYO97_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1754109.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2313..2738
                     /gene="lacA"
                     /locus_tag="VYO97_001324"
     CDS             2313..2738
                     /gene="lacA"
                     /locus_tag="VYO97_001324"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1754110.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2769..3284
                     /gene="lacB"
                     /locus_tag="VYO97_001325"
     CDS             2769..3284
                     /gene="lacB"
                     /locus_tag="VYO97_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1754111.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3295..4224
                     /locus_tag="VYO97_001326"
     CDS             3295..4224
                     /locus_tag="VYO97_001326"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1754112.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYCASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFTKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4226..5206
                     /gene="lacD"
                     /locus_tag="VYO97_001327"
     CDS             4226..5206
                     /gene="lacD"
                     /locus_tag="VYO97_001327"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1754113.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5440..6276
                     /locus_tag="VYO97_001328"
     CDS             5440..6276
                     /locus_tag="VYO97_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1754114.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6312..6629
                     /locus_tag="VYO97_001329"
     CDS             6312..6629
                     /locus_tag="VYO97_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1754115.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6629..8320
                     /locus_tag="VYO97_001330"
     CDS             6629..8320
                     /locus_tag="VYO97_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1754116.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDVAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8535..9941
                     /gene="lacG"
                     /locus_tag="VYO97_001331"
     CDS             8535..9941
                     /gene="lacG"
                     /locus_tag="VYO97_001331"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1754117.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNVKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9984..10193
                     /locus_tag="VYO97_001332"
     CDS             9984..10193
                     /locus_tag="VYO97_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1754118.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEE"
     gene            complement(10520..11281)
                     /locus_tag="VYO97_001333"
     CDS             complement(10520..11281)
                     /locus_tag="VYO97_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1754119.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(11463..12425)
                     /gene="nrdF"
                     /locus_tag="VYO97_001334"
     CDS             complement(11463..12425)
                     /gene="nrdF"
                     /locus_tag="VYO97_001334"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1754120.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADD
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEVMQDDDYNYGLD"
     gene            complement(12613..14772)
                     /gene="nrdE"
                     /locus_tag="VYO97_001335"
     CDS             complement(12613..14772)
                     /gene="nrdE"
                     /locus_tag="VYO97_001335"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1754121.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(14853..15071)
                     /gene="nrdH"
                     /locus_tag="VYO97_001336"
     CDS             complement(14853..15071)
                     /gene="nrdH"
                     /locus_tag="VYO97_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1754122.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         4673 a         2721 c         3095 g         4599 t
ORIGIN      
        1 aagggaaaac cttatggtat tagattattt ctttgacaaa aaccttgtgt tttgcttaga
       61 agcggataat caagaacaac tctttgatca ggtagcaagc ttgttggaag aacgagaaat
      121 tgtcactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc caactggttt
      181 agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcaca cagatattgt
      241 tcataatcta gctgaaaaag tggtggttgt tcgattagaa aaaccagtaa cttttcacaa
      301 tatgattgcg ccaggtaagg aagtagaagt atccctactc ttctttatca ttaacaattc
      361 aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag gaaatggaca
      421 tcttgaagac ctatcaaaaa tttccgaacc agaaaaactt tatgcttaca ttgctgaagc
      481 aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatctaaatg attaaaattc
      541 ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct ctagaagaag
      601 aactttcaaa ccgtggttat gatgttcact gtgatgcagt tatggtgaaa gatgtaaacg
      661 aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac cttggttttg
      721 aaccaggtat cccagttatc gaagctgggc caatcttatt ctgtatccca gcaatgagcg
      781 ttccagtatt tgacaatatt agacttcctg cgaaacaaaa tatggtatag tagttccatg
      841 aattatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt tggtgttcaa
      901 cgcacgactt ttgaagagat attagctgta ttaaaaacag cttatcaact taaacacgca
      961 aaaggtggac gaaaacctaa attaagccta gaagactttc ttatggccac tcttcaatat
     1021 gtgcgagaat atcgaactta tgaagaaatt gcggctgatt ttggtatcca cgaaagcaac
     1081 ttaatccgtc ggagccaatg ggttgaagta actcttgttc aaagtggtgt tacgatttca
     1141 agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt acaaatcaat
     1201 cgccctaaaa aaacaattag cgaattattc tggtaaaaag aaatttcacg ctatgaaggc
     1261 tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcgctg tgaactattg
     1321 tcatgatatg aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt
     1381 ggctgacagt ggttatcaag ggctcatgaa gatatatcct caagcacaaa ttccacgtaa
     1441 atccagcaaa ctcaagccgc taatagctga agataaagcc tataaccatg cgctatctaa
     1501 ggatagaagc aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgttttcaac
     1561 aacctatcga aatcatcgta aacgcttcgg attacgaatg aatttgagtg ctggtattat
     1621 caatcatgaa ctaggattct agttttgcag gaagtctaat aaagaaacta gcaaggtcag
     1681 gccttacttt attggtggac atccaggatt taattgtctt ctacttgatg atgaagtcta
     1741 tgaagattat tatctagagt ttgagaaaga agagatttgc tctgttccac gtcctttccc
     1801 agaaacaggt atgttggatt tccaagatag aagtccatgg ctagagggac aaaaagaaat
     1861 agatcttagt tatgatcttt tcagcacaga tgcagtgact ttggatgaat tgcaatctcg
     1921 aacaattgcc cttcgttctc ttaaacatga taagggattg aaagtgcatt ttgcagaatt
     1981 tccaaacctc atcatctggt caactttgaa caagggacct ttcattacct ttgaaccatg
     2041 gtctggcttg tcaacattcc ttgaagaagg agatcattta gaagataaga agaatgtttg
     2101 tctcttagaa gccaatcagg ttgaagaatt agggtttgag atagaagttt tataaaaaaa
     2161 gtaaaaaata aataacaaaa acaaacacaa actgttgact tcttcaaaca atagtagtat
     2221 attatgtttg tgaggactaa ttgactgttt tttaaagaca gccaatacac tgctggaatc
     2281 cagcatagaa aaaataaaaa ggagtataca atatgtctat tgttatcggt gcagatgctg
     2341 caggtttgag attgaaagaa gttgtgaaag acttcttaga aaaagaaaac ttccaccttg
     2401 tggatgttac agctgaaggt caagactttg ttgatgtgac tctcgctgtt gctgcagaag
     2461 taaacaaaga agaacaaaac cttggtatcg tgattgatgc ttatggagct ggtccattta
     2521 tggttgcaac taagatcaaa ggaatggttg ctgcagaagt atctgacgaa cgttcagctt
     2581 atatgactcg tggccacaac aactcacgta tgatcactat gggggcacaa cttgttggtg
     2641 atgaattggc taaaaacatc gctaaaggat ttgttaatgg taaatacgac ggtggtcgtc
     2701 accaaatccg ggttgacatg ttgaacaaaa tgtgctaatt agattatagt aagaaaggta
     2761 agttaaaaat gagaattgca attggatgtg accacatcgt aacagatgaa aaaatggcgg
     2821 tttcagaatt tttgaaatca aaaggatatg aagtcattga ctttggtacc tatgaccata
     2881 cacgtactca ctacccaatc tttggtaaaa aagtagggga agcagtaact agtggtcaag
     2941 ctgatctcgg ggtatgtatc tgtggtactg gtgttggtat caacaacgct gtaaataaag
     3001 ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac agccctttat gctaaagaac
     3061 aattgaacgc caacgttatc ggttttggtg gtaagattac tggtgaattg cttatgtgtg
     3121 atatcatcga agctttcatt catgctgaat acaaaccatc tgaagaaaac aaaaaattga
     3181 ttgcgaaaat tgaacacctt gaaagtcaca atgctcaaca aacagacgca aacttcttca
     3241 ctgaattcct tgaaaaatgg gatcgtggag aataccacga ctaagaggta acctatgatt
     3301 ttaacagtta caatgaaccc atccattgat atttcctatc ccttggatga gttgaagatt
     3361 gatactgtca atcgtgtggt ggatgtaacc aaaacggctg gtggtaaggg actcaatgtt
     3421 acccgagtac tttcagaatt tggcgattct gttcttgcta ctggtttagt gggtggcaaa
     3481 cttggtgagt ttttggttga acatatcgat aatcaagtaa agaaagattt cttctcaatt
     3541 cagggagaaa ctcgtaactg tatcgctatt ctccacggag acaaccaaac agaagttctt
     3601 gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagact ttttggaaca tttcaaaaaa
     3661 ctcttggagt cagttgaagt agtggccatc tcaggtagtc tgccagctgg ccttccagtt
     3721 gattactgtg cgagcttggt agaacttgct aatcaagctg gcaagcctgt agtcttggac
     3781 tgctcaggtg cagcacttca ggctgttctt gaatcacccc ataaaccaac agtcatcaaa
     3841 ccaaataatg aagaattgtc tcagcttctt ggaagagaag tttctgagga tttggatgaa
     3901 ttaaaagaag tacttcaaga acctttgttt gcagggattg aatggattat cgtttcactt
     3961 ggtgccaacg gtacttttac caaacatggt gacactttct acaaggtaga tattcctaga
     4021 attcaggtgg taaatcctgt tggatctgga gactctactg tggcaggaat ttcttcagga
     4081 cttcttcaca aagaatcgga tgcagaatta ctcatcaagg caaatgtcct tggtatgctc
     4141 aatgctcaag aaaaaatgac tggtcatgtc aacatggcca actatcaagc tctatatgat
     4201 caattaatag taaaagaggt ataaaatggc tttaacagaa caaaaacgtg tacgcttaga
     4261 aaaactttct gatgaaaatg gtatcatctc agctcttgca tttgaccaac gtggtgcttt
     4321 gaaacgcctc atggctcaac accaaacaga agaaccaact gtggcccaaa tggaagaact
     4381 taaagttttg gtagcagatg aattgactaa atacgcttca tctatgcttc ttgaccctga
     4441 gtatggactt ccagcaacta aagctcttga tgaaaaagct ggtcttctcc ttgcttatga
     4501 aaaaacaggt tatgacacaa caagcacaaa acgcttgcca gactgcttgg atgtttggtc
     4561 tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa ttcttgcttt actatgatgt
     4621 agatagctca gacgaactca atcaagaaaa acaagcctac atcgaacgca tcggttctga
     4681 gtgtgtggct gaagatatcc cattcttcct tgaaatcctt gcttacgatg aaaaaattgc
     4741 agacgcaggt tctgtagaat acgctaaagt aaaaccacac aaagttatcg gtgctatgaa
     4801 agtcttttca gacccacgct ttaacattga tgtcttgaaa gttgaagttc ctgttaacat
     4861 taaatatgtt gaaggcttcg ctgagggtga agtggtttac acacgtgaag aagcagcagc
     4921 cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac atctacttga gtgctggtgt
     4981 atcagctaaa ctcttccaag atactcttgt atttgctcat gaatcaggtg caaactttaa
     5041 cggagttctt tgtggccgtg ctacatgggc agggtcagtt gaagcttaca tcaaagatgg
     5101 tgaagcagca gctcgcgaat ggcttcgcac aactggattt gaaaacattg atgagctcaa
     5161 taaagttctt caaacaacag cgacttcatg gaaagaacgt gtgtaagaaa gtcctcctag
     5221 tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta tgtaaaggtt tacaaaataa
     5281 cttacttgtg ctatacttaa atcacaagtt aatacaaggt gagtgttact aagtaatatt
     5341 aggcatgatc acaggtgaat tagaaatcag ctgattttct agttcatttg tggtcatttt
     5401 ttgtactcat atacctttaa aatataaaag gaggttgaca tgtatcgaat tctaaatcca
     5461 atgaatcaca atgtctcgct tgtcagaaat gataagggag aagaggtgat tgtaattggt
     5521 aaggggattg cattcggaaa gaagaagggg gatttgattg ctgaaaatca ggttgagaaa
     5581 atctttcgga tgaagaccga agagtccaga gaaaacttta tggctcttct caaagatgtt
     5641 ccgcttgatt ttatcacagt gacctatgaa atcattgata agctatcaaa gaaatatcat
     5701 tatccgattc aagagtatct ctatgtaacc ttgacagatc atatttactg ttcttatcaa
     5761 gctctaactc aaggaaggta caaggatagt aatctgccag atatttccgc taagtatcct
     5821 gtcgcttttc aaatcgcaaa tgaagctttt gaaatttacc gtcagaagct agcagatcat
     5881 tttcctgagg acgaaattat tcggattgct tatcatttca ttaatgctga aggtgaaaat
     5941 gaagtggaac ttgtggagtc gattgataag aggaaagaaa ttctcaggaa tgttgaagaa
     6001 gttttaacgg actatgcaat tcaacgaact aaaaagaata accatttcta tgatcgcttt
     6061 atgatccatt tgaattattt cttggattat ttagacagat ctagagatga taaccaatca
     6121 cttctggata tggaagatca tattaaacaa tcctatccaa aagccttcga gattggttcc
     6181 aagatctatg atgtgattac gcaacatacg ggtcttgatt tgtataaaag tgaacgagtt
     6241 tatctagttc tacatatcca acgtttattg tcataaaaat ttatttaaaa ctatataagg
     6301 agaattctat catgaataga gaagaagtaa cattgttagg ttttgaaatc gtagcctatg
     6361 ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc tgctgaagct ggtgattttg
     6421 aaaaagcgga cgctctggta gaggaagctg gtagctgtat tgcagaggct caccacgcgc
     6481 aaacaagtct attgactaag gaagcttctg gtgaggactt agcgtatagt gtgaccatga
     6541 tgcatggtca agatcacttg atgacaacta tcttgttaaa agacctaatg caccatttaa
     6601 ttgaacttta caagagagga gttaaataat gaataaacta attgcattta tcgagaaagg
     6661 aaagcctttc tttgaaaaac tatctcgtaa tatctatctt cgtgctattc gtgatggttt
     6721 cattgcaggt atgcctgtta ttctcttctc aagtatcttt atcttgattg cctttgtacc
     6781 aaactcatgg ggctttaaat ggtctgatga agttgtagcc tttctgatga aaccttatag
     6841 ctattctatg ggtattctgg ctctcttggt agctggtaca acagctaagt cattgactga
     6901 ctcagtaaac cggagcatgg aaaaaaccaa tcaaatcaac tatatgtcaa cattgttggc
     6961 agcaattgtt ggtttgttga tgttggcagc tgatcctatc gaaagtggtc tagctactgg
     7021 attcttggga acaaaaggtt tgctttcagc cttccttgct gccttcgtta ctgtagccat
     7081 ctataaggtc tgtgttaaga acaacgtcac tattcgtatg cctgacgaag ttccaccaaa
     7141 tatctcacaa gtctttaaag atgtgattcc attcactcta tcagtggttt ctctttatgc
     7201 tcttgattta ctagctcgtc attttgttgg ttctagtgtg gcagaatcaa tcggtaaatt
     7261 cttcgcacca ctcttctcag cagcagacgg ataccttggt attaccatta tctttggtgc
     7321 ctttgccttc ttctggtttg ttgggattca tggtccatct atcgttgaac ctgctatcgc
     7381 agcgattacc tatgcaaatg ctgaagtcaa cttgaacctt ctccaacaag ggatgcatgc
     7441 agacaagatt cttacttctg gtacacaaat gtttatcgtt accatgggtg gtacaggtgc
     7501 gacattggtc gttccattta tgttcatgtg gttgacaaaa tcgaaacgta accgtgcaat
     7561 cggacgtgct tcagtagttc ctaccttctt cggtgtaaat gaaccaatct tgtttggtgc
     7621 acctcttgtt ttgaatccaa tcttcttcat tccatttatc tttgctccaa ttgcaaacgt
     7681 atggattttc aaattcttta ttgaaactct tggaatgaac tcattcactg ctaatctacc
     7741 atggacaact ccagctccac taggtctagt tcttggaact aacttccaag tgctatcatt
     7801 cattcttgct gcccttctaa ttgtggttga cgtagttatt tattacccat tccttaaagt
     7861 ctatgatgaa caaattcttg aagaagaacg ttcaggtaag tctaatgatg aattgaaaga
     7921 aaaagttgct gcaaacttca acactgcaaa agcggatgct attcttgaaa aagcgggtgt
     7981 cgatgtagca caaaatacca tcactgaaga aacaaatgtc ctcgttctct gtgctggtgg
     8041 aggtacaagt ggactccttg caaatgcttt gaataaggca gcagcagaat acaatgtccc
     8101 tgtgaaagca gcagcaggcg gctatggtgc tcaccgtgaa atgttgccag agtttgattt
     8161 ggttatcctt gctcctcaag ttgcttcaaa ctttgaagat atgaaggcag aaacagataa
     8221 gctcggtatt aaacttgcta agacagaagg cgctcaatac atcaaattaa ctcgtgatgg
     8281 aaaaggtgcc ctagcattcg tacaagagca attcgattaa ggctaggaac tgtgaaacag
     8341 tctcccatcg ttacggaaat cgctatggcg aatttcctaa tcgccttatt aattcgtcgg
     8401 taaaaagata tcgtttttac ctcctcatgt cacaattcag tgacttggta caagaagtga
     8461 gatggagaag catggctcac tgactcctct cctctcactt ttactttatt taaatcaaga
     8521 aataggtgaa aaaaatgaca aaaacacttc caaaagactt tattttcggt ggcgcaacag
     8581 ctgcttatca agcagaaggt gctacacata ctgatggaaa aggaccagtt gcttgggata
     8641 aatatcttga ggataactac tggtacactg ccgaaccagc tagtgatttt tacaatcgat
     8701 atccagttga cctcaagcta gcagaagagt atggtgtcaa tggtattcga atttctattg
     8761 cttggtcacg tattttcccg actggttacg gccaagtaaa tgttaaaggt gttgagtttt
     8821 atcataattt atttgcagag tgtcacaaac gtcatgttga gccctttgta actcttcatc
     8881 actttgacac gccagaagct ctccactcaa atggagactt cttaaaccgt gaaaatatcg
     8941 aacattttgt agactacgct gccttctgtt ttgaagaatt tccagaagta aactattgga
     9001 caacctttaa tgaaattgga ccaatcggtg atggtcaata tttggttggg aaattccctc
     9061 caggtatcca gtacgacctt gccaaagtct ttcaatcaca ccacaatatg atggtgtctc
     9121 atgcgcgtgc agtcaagcta tataaagata aaggctataa aggtgaaatt ggtgttgttc
     9181 acgctctgcc aaccaagtat cctttggatc ctaaaaatcc agcagatgtt cgtgcagctg
     9241 agttggaaga tatcatccac aataaattca tcttagacgc aacttatcta ggtcgctatt
     9301 cagctgaaac catggaaggt gtcaaccata tcttatcagt caatggtggt agtttggatc
     9361 ttcgtgaaga agattttaca gcattagaag ctgcaaaaga cttgaatgat ttcctaggaa
     9421 tcaactacta tatgagtgac tggatggaag cctttgatgg agaaactgaa attatccata
     9481 atggtaaagg tgaaaaagga agctctaagt atcaaatcaa aggtgttggt cgtcgtgtag
     9541 ctcctgacta tgtaccacgc acggattggg attggattat ctaccctcaa ggtttgtatg
     9601 accaaatcat gcgtgtgaag aaagattatc ctaactacaa gaagatttac atcactgaaa
     9661 atggtctcgg ctataaagat gagttcgttg ataacactgt ttacgatgat ggtcgtattg
     9721 attacgtgaa acaacacttg gagatcttgt ctgatgcgat tgcagatgga gctaatgtaa
     9781 aaggttactt catttggtca ttaatggatg tcttctcatg gtcaaacggt tatgagaaac
     9841 gttatggtct cttctacgta gattttgaaa ctcaagaacg ttatcctaag aaatcagctc
     9901 actggtacaa gaaagtagcg gaaactcaga ttatagacta gtagaattag tcattagata
     9961 tagaatttta gtgagtcaaa aagatgttca aagattttat ccaatctatt tatgaaaaag
    10021 tttatattat aaatttcgaa aaatgctctc aaataccgtg tttgacgagt gaagaattga
    10081 aaagtcttgg aaaatggtat gtctcgactg gtaaagaatg gatttgtcat tcagatgatg
    10141 agctggaaga atttaaaaat ctatttttaa attttatcaa tcctgaagaa taggatacta
    10201 tctcctttga ttcagatttt atgccgtttc aacaatcgta accaatttct caaaaaagtt
    10261 aaatcttata tttagtactc tgcaaaactc ttatctgatc acgttgctta tactcaatga
    10321 aaatcaaaga gcaactttaa actaggaagc gagtcgcaga tttctcaatg catagctttg
    10381 aggaattggg caaaaagtct ttgatataga aaaaagcata gtatcaggtg ttgaaacacc
    10441 tgatactatg ctttttattg tgggaagatt tacttttttt cttctgaaat tgagttgtta
    10501 cccaggctct ttcagtttat taaggcttga tgactttaat gtgtttagat agcttaaaaa
    10561 ggattgaatc acttagttta gaatctgaaa caatagtatc aagatttgat acattataaa
    10621 aagtataaaa atcaaactta ttgaacttac tatgatctgc gagtaaatat tttttattag
    10681 aattatttaa agcgatgcgt tgagcctctc cctcttcctc gctaaaagta gctagagctc
    10741 cgttttgaat accattacag ctaacgaaag ctttagaaaa ttggagatta gagagatttt
    10801 gtagggtcaa tgtaccaaca aaagcacctg taatatcgcg ataatttcca cctattaaaa
    10861 tcaaatctgt taattttcgt tcgcttaaaa tcagaaaaac aggtagactg ttggttacga
    10921 cgcggatatt gtcaataggc aactcacgcg caaaaaactc taatgttgtt cctggtccaa
    10981 tgaaaatagt ttctctttct tctactagac tgcctgcaaa atgggctatt tcttgttttt
    11041 ctgccgtttg gagggcttgt ttttcaatat ttgatcgctc attagtcaaa agggagttgg
    11101 ttcgaagttt ttcagctcca ccatgcacac gaatcagcaa atctttatca gctaattcct
    11161 gtaaatagcg ccttgcagtc atatctgaaa cggctatttc gtccataatc tgtttaactg
    11221 ttatggttcc tttactattt actatctcta aaattttggc taatttttct tgtttgagca
    11281 tattatcacc tcgtttccta ctactatctt accataaaca aactcatcat tcaaatacaa
    11341 aaacaacaaa atgaaacaaa aacaaaaata tcgaagtttg ttttcaaaac tttcgatatt
    11401 tttgttgggt tataactttg atgtttctag tttacttttt gatgattgag agtgatggag
    11461 aattagtcta aaccgtagtt atagtcatcg tcttgcatga cttcaacttc gccaagaagg
    11521 taaccatttc cgacttgaga gaagaagtcg tggttggaag ttcctgttga aataccgttc
    11581 attacgattg ggttgacatc atcagctgaa tctgggaaga gtggatcttg tcccatgttc
    11641 atgagagctt tattggcatt gtagcgaagg aaggttttaa cctcttcagt ccaaccaaca
    11701 ccgtcataaa gactctctgt gtagccttct tcattttcat aaagagtata gagtaggtcg
    11761 tacatccatt ctttgagttt ttcttgctct tcttcaggta attcattgaa accaagttgg
    11821 aatttgtaac caatgtaggt tccgtgaaca gactcgtcac gaataatcaa tttaatgatt
    11881 tctgcaacgt tggcaagttt gttgttaccg agatagtaga ggggagtgaa gaaaccagag
    11941 tagaagagga aggtttcgag gaagacgctg gcaactttct tttcaagtgg gctgccgttt
    12001 aggtagattt cgttgacaat ctcagccttc ttttgtaggt aaggattggt attggtccat
    12061 tcgaaaattt cttcaatctc agccttagta ttcaaggtag aaaagattga tgagtaagat
    12121 ttagcgtgga cagattccat aaattggatg ttattgaaaa cagcttcctc atgtggtgta
    12181 cggatgtctg cgcgaagggc ttgaacccca gtttcagatt gcatagtgtc aagaagggtt
    12241 aaaccaccaa aaacttttcc gaccaagtct ttctctttgt tagatagctt tctccagtca
    12301 tccaagtcgt ttgataaggg aatacgtgta tcgagccaaa attgctccgt cagtttttcc
    12361 caagttgatt tgtcgatgac atcttcgatg gcattccagt taatggcttt gtagtaagtt
    12421 tccatttaaa atctctttct gtgtttagta ttgcgaactc acaattattt ctactttacc
    12481 ataattctat aggagtatcg cacaaaaagt cggaagcccg acttttaaaa tgttacataa
    12541 attatgttat gacatagtag atttgatttt atcagtgctg cttagggaaa aatagtgttt
    12601 ttatgctaga aactaaatca cacagctttc acattggttg gcgccgactt ctccaccgtc
    12661 atctgtaaag gtacggacgt agtagataga cttgattccc ttgttaaagg catagttacg
    12721 aaggatggac aagtcacgtg tcgtttgttt attttccctc ttccattcgt aaaggccttt
    12781 tggaatgtca ctgcgcatga agagggtgag tgaaagtcct tgatccacgt gttcagtcgc
    12841 agcagcgtaa acatcgatga ctttacgcat atccatatcg taggcagaag tgtagtaagg
    12901 aatggtttct gtagacaagc cagcagcagg gtagtagatt ttaccaattt tcttctcttg
    12961 gcgttcttcg atacgttgcg taatcgggtg gatagaagca gaaacgtcgt tgatatagct
    13021 gatagaacca tttggcgcta cagcaaggcg attttggtgg taaagaccat cttcttgaac
    13081 cttgtcgcga agttcagccc aatcagcaac accagggata aaaacatttt tgaagagttc
    13141 tttaacacgg tctgatgttg gaacaaattc accagttaca tacttgtcaa agtaacttcc
    13201 gttagcatag tctgattttt caaagttgtg gaaggtaata ccacgctcac gcgcaatatt
    13261 gtttgactct accaaggtcc agtagttcat aagcataaag tagatgcttg taaattcaac
    13321 agactcaggt gaaccatatt caatgagttg ttgggcaagg tagctgtgca gtcccatggc
    13381 accgagacca aaggtgtggg cttggctatt tccatggtca atcgttggta cagctacgat
    13441 atgtgaacta tctgtaacga aagtaagggc acgaaccata gcacggatag aacgaccaaa
    13501 atcaggtgaa gtcatcatgt taaccacgtt ggttgaaccc agattacatg aaacatctgt
    13561 tcccatttga aggaattctt gagcatcgtt gatcaagctt ggttcttgaa cttgaagaat
    13621 ctcagaacac aagttactca tgataatctt tccatcaaca ggatttgcac ggttagctgt
    13681 atcgatgttg actacataag gatagccaga ctcttgttgc aatttagaga tttcagtttc
    13741 caaatcccgc gccttgattt ttgtcttgcg aatatttgga tttgcgacca attcatcgta
    13801 tttttcagta atgtcgatgt aattgaatgg cacaccgtat tctttttcta cagagtaagg
    13861 gctgaagagg tacatttctt catttttacg agccaattcg tagaatttat caggtactac
    13921 aacaccaagt gatagagtct tgacacgtac tttttcatca gcgttttctt tcttagttga
    13981 aaggaaagcg atgatatctg ggtgaaagac gttgaggtag acaacaccgg caccttgacg
    14041 ttgccccaat tggttggagt aagagaagct gtcttcaaaa agcttcataa caggaacgac
    14101 acctgaagca gctccttcat agcctttgat aggtgcacca gcttcacgaa ggttgctgag
    14161 ggtaattccc acaccaccac caatacgtga aagttgaaga gctgagttga tagaacgtcc
    14221 gatagagttc atatcatccg tcacttggat taggaaacaa gataccaact ccccacgacg
    14281 agcacgtcca gcattcaaga aggaaggagt agcaggttgg tagcgttggt ggatgatttc
    14341 attggcaata tcgattgcaa cagcttcatt cccatcagcg aaataaaggg cgttgaagaa
    14401 gacacggtct tccatatttt caagatagta ttcaccgtca ttagtcttta aggcatattg
    14461 attgtaaaat ttataagctg ccatgaatga cttgaattgg aagttttggt ctttgataaa
    14521 ttgagccaat tcttccaaga actctggacg gtatttcttg ataaaggctg tttcgatgta
    14581 gttgtgttca atgaggtaat tgattttgtc tttgattgaa tcaaaaacca tagtgtttgg
    14641 aactacattt tctttaaaga aagcatccaa ggcttcctta tctttatgaa gcatgatttg
    14701 tccattaaca ggacggttaa tttcgttatt aagacggaag taagtcacgt cttcaagatg
    14761 ttttaatccc ataaaatttc ccttatctaa ttacaaaaga aaggcttcta agttagccct
    14821 aaaagcagtt tcttctggat gatgtactaa gattatgcta attgtttcag ttttcctggt
    14881 tggaaacctg aaaagacttc agttggtgtt tggataacag gagctgcgct aaaaccgagc
    14941 tctttaactt gatcgacgta ctcaggttgc tcatcaagat tgatttcacg ataagagaca
    15001 ttattactgt ccaagaaacg cttggtcatt ttacattgga cacaattgtt tttagaataa
    15061 acggttacca ttgtgtaact cctcttca
//
LOCUS       DAVMAF010000041        14326 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMAF010000041 DAVMAF010000000
VERSION     DAVMAF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.041"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(527..1507)
                     /locus_tag="VYO97_001337"
     CDS             complement(527..1507)
                     /locus_tag="VYO97_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1754123.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1845..1994)
                     /locus_tag="VYO97_001338"
     CDS             complement(1845..1994)
                     /locus_tag="VYO97_001338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754124.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            complement(2022..4037)
                     /gene="recG"
                     /locus_tag="VYO97_001339"
     CDS             complement(2022..4037)
                     /gene="recG"
                     /locus_tag="VYO97_001339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1754125.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(4056..5159)
                     /locus_tag="VYO97_001340"
     CDS             complement(4056..5159)
                     /locus_tag="VYO97_001340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1754126.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(5149..5517)
                     /locus_tag="VYO97_001341"
     CDS             complement(5149..5517)
                     /locus_tag="VYO97_001341"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1754127.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5556..6587)
                     /locus_tag="VYO97_001342"
     CDS             complement(5556..6587)
                     /locus_tag="VYO97_001342"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1754128.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6589..7620)
                     /locus_tag="VYO97_001343"
     CDS             complement(6589..7620)
                     /locus_tag="VYO97_001343"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1754129.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7701..10214)
                     /gene="secA"
                     /locus_tag="VYO97_001344"
     CDS             complement(7701..10214)
                     /gene="secA"
                     /locus_tag="VYO97_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1754130.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10360..10647)
                     /locus_tag="VYO97_001345"
     CDS             complement(10360..10647)
                     /locus_tag="VYO97_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754131.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10658..11308)
                     /locus_tag="VYO97_001346"
     CDS             complement(10658..11308)
                     /locus_tag="VYO97_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754132.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(11312..11953)
                     /locus_tag="VYO97_001347"
     CDS             complement(11312..11953)
                     /locus_tag="VYO97_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754133.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11946..12737)
                     /locus_tag="VYO97_001348"
     CDS             complement(11946..12737)
                     /locus_tag="VYO97_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754134.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12759..13151)
                     /locus_tag="VYO97_001349"
     CDS             complement(12759..13151)
                     /locus_tag="VYO97_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754135.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAVDMARQNWWDLSAAISGSWSPN"
     gene            13478..>14326
                     /locus_tag="VYO97_001350"
     CDS             13478..>14326
                     /locus_tag="VYO97_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1754136.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLSEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKVFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
BASE COUNT         4301 a         3196 c         2612 g         4217 t
ORIGIN      
        1 tgtttttata ctcaatgaaa atcaaagagc aaactaagaa gctagccgca tgatgctcaa
       61 aacactgttt tgaggttgta gataagactg acaaagtcag ctcaaaacac cgttttgagg
      121 tggcagatag aactgacgaa gtcagtaaca tatatacggc aaggcgaagc tgacgtggtt
      181 tgaagagact tttgaaaagt attaacactt acagacaagc ctgacgatag caagaactac
      241 cctactcgat aggtatcggc ttttgctttc tgaaaaaaat tattttaagc aatttgacaa
      301 atctagcaac aaaaaattct ataaatataa tagattgaaa ctagaatagt acacctttgt
      361 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc aatttgtcct gttcttgttt
      421 cattttacta tatttctatg ataaaacgca tagtatcaag ttttcttaat cccctgatac
      481 tatgcgtgtt tgtaattttt aagattttgt acttagagtc gactccttat tttagatatt
      541 taaaaggaat ctcacttcca cagagccagt tgtagacttg gtcattgaca aatacattca
      601 tggcttcgtg agcatactca ggcatgatac gataggtttt atcgcaggtc agacgattat
      661 aaatcgcaaa ctgggtaatg ggatagcaaa catcgtcgtc caagcccgta atcatcttaa
      721 cctcaccttg gatacgatgg gcaagatttt tgacatcgat ataggcaagg gtcgccatga
      781 tttcctcctc tgtttcatgg aaggggtcgt gaaacttgaa ataacggaaa agttcgtcgt
      841 aagcctcgct agtattacca atctcaatca cccgtctgaa gtctgacaag aagggataaa
      901 tggcaactgt tttctgaatt cgaggattga gggctgctgc aactagggct agagcccctc
      961 cttgtgaggc accatagcta gaaagacgct tctcatcaac ctgagacaga ctagcaacaa
     1021 tttcgaccaa ctggtaaata tccagataaa catccttata aaagaggtgg tcccgacctt
     1081 ccacagcacc acggataata tgccccttca cggtatttcc taaaggagaa cgcaagccgt
     1141 cttgtgagta acctgactgg ccccgcacat ccatggaaac aacaccgtaa ccagctacgg
     1201 tgaagcccag catgtcggcc cagtcccagc cacgtcccat ataaccatga aaatggaaga
     1261 ttagtggaac cttctcctca ctctttggaa gaacaatgcg tgcatagacc tttccttcct
     1321 tgcttccttc aaatgttaac tcatagcact tgacttgagg aatgtggaaa tctctttcct
     1381 ccaagtggta ggatggaagc gtggaaacat ttttcacttc cccatcccag aaagcatcaa
     1441 agtcttccgg aacctcatcc cttcctctat aggtcttaat ttcttctagc aaagctggat
     1501 ttttcatagc atgctccttt tattttgtaa tcgttaccac aactgtagca tatctatgta
     1561 atcaatgcaa gaaagaaggg taaatagaaa gtgaatttag attctttact tttaggatga
     1621 tgggctgaaa gtagttttag aaacgaaaaa agagcatttc gctctctctt ctgtttatac
     1681 tagttttttg cttccttctt ttggaaagtg gtttggtaat ttactttaat cgttactgtc
     1741 atatgagagt cctcgtttct ttttgatgac tttagtataa gagccaaacc taaaaactag
     1801 cttaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tatatatatg
     1861 ttactgactt cgtcagttct atctgccacc tcaaaacggt gttttgagct gacttcgtca
     1921 gtttcatcca caacctcaaa acagtgtttt gagcaacctg cggttagctt cctagtttgc
     1981 tctttgattt tcattgagta taagttttcc ttagagaaag cttaatccag atgttctttc
     2041 ttttccagat gaagggcaat catgcgccac tctggatctt cttgccaagc ttctatagaa
     2101 ctaatgtagc tagcaacctt tcttgcttct tctaaaatcg gaaaatcttc gataatatca
     2161 gccacttgga actctggaag tcctgactgt ctggttccaa aaatctcacc agaaccacgc
     2221 attttcaaat cttcctccgc aaggacaaat ccattggtcg tttctgtcat gatgcgcatg
     2281 cggtctttcc cagaatccgt cttgggattg gcaacgagaa cagcgtagga ctgcttgtcc
     2341 ccccgaccga cacgacctct aagctggtga agctggctga gaccgaagcg atcggcatcc
     2401 atgataatca tgacagtcgc attgggaacg ttgaccccaa cctcaataac cgtcgtcgaa
     2461 accagaatat ccgtctttct ctccttgaaa tcctgcatga tctggtcttt ttcgtcactc
     2521 ttcatcctac catgtagaag agccacctct gccttgcctg caaaatgagt cgtcaactcc
     2581 tctgataagg caatggcatt tttcaaatct agagcttctg attcttcaat caaaggagag
     2641 atgacatagg cttgggaacc tttttgaatt tccccctcta accaagtcaa gacctgaggt
     2701 agttgctcat gtttgatcca gcgcgtcaca ataggcttcc gacctgctgg catctggtcg
     2761 ataatggaaa catccatatc tccaaaggct gtgatggcaa gcgtccgtgg aatgggagtc
     2821 gccgtcatca tgaggacatc tggattgtca cctttttccc gtaaaatacg cctttgccct
     2881 acaccaaaac ggtgctgctc atcgataata atcaaaccaa gacgagcata ctccacccca
     2941 tcttgtatca gagcgtgagt tccgataatc aaatcagcct cacccttggc aatggtctcc
     3001 aagacttctc tcttttctgc agctttcaag gaacctgtca agagagccag tttcaaattg
     3061 ggaaaaaggt tctgtaaact ctcaaagtgt tgctctgcga ggatttctgt tggtaccatt
     3121 agggcagcct gataacctgc tgtcactgcc gcaaacatgg ccaagccagc gactaccgtt
     3181 tttccactcc ccacatcccc ttgtaggaga cgattcatgt ggtggtcgga cttcatatca
     3241 gttaaaattt cctgcaaact cttttcctga gcttgggtca gggcaaaagg aagacttact
     3301 ttaactgctg tcactttttc ctgagaccaa ttcagaacca gaccacttcc ctgaactcta
     3361 ttttcagact tgagcgtctg caactgcatt tggaaataaa agagttcctc aaacttgata
     3421 cggcgaagag cctgcttgta ttctgccaaa tcctttggaa aatgcatagc tcggactgcc
     3481 tgacaacggg acatgagttt gtatttgtct agtaaagact ggggcagatt ttcttctatc
     3541 aagaggtcca gtccctgatc aaaagccgtc ttgatgacct taaccagact ggcctgactg
     3601 attccctgag ccagacgata gacaggctgg aggtcatctt ctacctgagc cagaaccttc
     3661 atcccagtca gactagcctt agcgcggtcc cattttccaa agacagcaag ggttgctccc
     3721 aactctattt tatcagccag atagggctgg ttaaagaaat tcaccgcaaa aacgacctct
     3781 ccctgcttga gactaaaacg caggcgattg cgcttgaaac cataatactg gacactagca
     3841 ggagtcacta cctgaccaga aagaactgcc ttctcaccgt cttccagctc cagcacctgc
     3901 ttggttttga agtcttcata acggaaagga aagtagagca agagatcttg caagttttca
     3961 attcctagtt tggcgtattt ttctgctgac tttggtccca caccaggcaa gacatgcaag
     4021 ggttgatgta gattcatgct ccactccttt cttttctaat aatattctct cggaatacgg
     4081 tcgctgagga ggcaaaccac ctcatagtta atggttacgc ggtaggtcgc tacctgagtt
     4141 gcagtgattt ccttatcccc attggagcca atcaaggtta ccttggttcc gagtgggtaa
     4201 ggcttaggca atcgaatagt gatttggtcc atcgaaaccc tgccgacaat tgggcaagct
     4261 tggccatcta ccaagacaga gaaattttgc atgtctcttg tccatccatc tgcatacccg
     4321 attggcacgg tcgcgatgac ttgctcgcta tccgcttgat aagttgctcc atagcccatg
     4381 caagctccag ctggaactgt cttgacatga accagagcag actccaaggt caaggccggt
     4441 atcaaatcat aaggcaaatc caagaccgct ccacttggat tgaggccata catggcatct
     4501 cccatacgaa ccgcattgaa aatagtctct acatgccaaa gagtcgttgc agaattgcta
     4561 gcatgaacca gctctggaac ttccttcata ctagctaaaa tagttttaaa ccgttctaac
     4621 tgggcattaa aatagtcatc tgattcctca tcagcagtag caaagtgggt aaagattcct
     4681 tcaacacgaa caccgtgttg ttggagcaaa tcttgagcct gctcaacctc actggcctct
     4741 ctaaaaccaa tccgtcccat ccctgaatca atcttgaggt ggactgtcaa tccagttagg
     4801 tccacttcct tatctaagag tgcttgaatc cactccagtc cagccactgt caaggtgaag
     4861 tcatattctt tagctagagc aacagcttcg atttcagaaa ctcctaaaat gaggattggc
     4921 ttgctgagtc cagcttgtct gagttcaatg gcttcatcga tattggaaac gcaaaagcca
     4981 tcaacatcat cttgaattgc cttggcaacg gcaacagctc catggccata agcattggcc
     5041 ttgaccacag cccacttgag cgttccttga gggatatgag cccccatttg ctgaatattt
     5101 tgtcgaatag ctcccagatg aatcagaacc ttggttggtc tatgtggact agctttcatg
     5161 attttcctcc aaaatgacac tggctgtcac aaactgatcg gtgtggctga tagacagcca
     5221 aatctttcct gaaaatggtg cctgactaaa ataaggcgcc ccacgttcat tgttcaagac
     5281 ttccaaatcc tgaaaaccga gcttgctaat gcccgttccc atagccttgg aaaaggcctc
     5341 cttagccgac cagcgaccag ctaaatattc tatttgcctg cgtcctttga gactggtgaa
     5401 gcgctccatt tcctgagcgg tcagtacacg cttagcaaat ccttcatgtc gtgtaactgc
     5461 gctttctatc gaagccaatt cttcgatgtc aattccgtgt ccaactatca ttctcatcaa
     5521 aaggagttga gattccccaa ctccatcctt ttcacttatt ttgtcaaggt aacataaatc
     5581 tcttctacca aggcctctgt attttcccaa cctaggcaag ggtcagtaat agagcaacca
     5641 aagacctctg gttggttttg acgaccatct gctaggtaag attcaatcat aaatcctcga
     5701 accgtctttt taattttctc attccaatca cgattctgca aggtctggcg aacaattcga
     5761 atctgctcca tatattgctt gcctgagtta tcatggttgg tgtcaatgag gataaaagga
     5821 ttttcaagtc ccatggtttc atatcgttca atggcttgga gtaggttttc atagtagtaa
     5881 ttcggcatat aattgccata ctcgttgact gctccacgga ggataacatg ggccaaagga
     5941 ttacctgatg tctcaacttc ctgcccatga taaaggaagg tttgcttgtt ttgagcagca
     6001 tagatggcgt taaacataac acccaaattt cctgaggttg gatttttcat ccctactggt
     6061 gcatcaatcc cagaagccac aaagcggtgc tcttggtctt ccacagaacg agctccaacg
     6121 gcatggtagc tgaccaagtc atccaccaag atcagatttg acggataaag catctcatct
     6181 gccgttgtca aaccagtctc tgtaatcacg cggtagtgca actggcgcac agcctgcaag
     6241 ccgttaatca ggcttggagc cttagaagta tctggctggt gaaccaatcc tttatagccg
     6301 tctccattgg tacgaggctt agcagtatac acgcgcatga ccatgaaaat cttgtccgct
     6361 accttctttt gcaaggcgga taaacggcga gcatattcca agaccgcctc ttcattatca
     6421 gaagagcaag gaccaatcac caagagaata cggtcatctt cccctgaaat aatatctgcc
     6481 aattccctgt cacggctttc cttgagtctc aaggcttctg cagacaattg ggtttcagcc
     6541 ttgatgactt ccatatcgat ttcttgacct ttttcaataa atgccatctt attctcctaa
     6601 cgttttgtaa atttctctga caagagcttc tgtgttatcc caacccaggc aagggtctgt
     6661 gatagactta ccaaatactt ctggctcatt ttgtcgacca tcttccagat aagactcaat
     6721 cataaaacca cgaacgaact gcttaatttt ttcattccaa gcacggttaa tcaaggtctg
     6781 gcggacaatt cggatctgtt caatatactg cttaccagaa ttgtcatgat tggtatcaat
     6841 gatgataaaa ggattttcca agcccatttt ctcatactgg gcaatggtat caattaaatt
     6901 gtcataatag tagttgggaa tattttttcc atactcatta agagcaccac gaagaatagc
     6961 gtgtgaaagc gggttcccag ttgtttctac ttcttttcct aagaaaagga aactttgttt
     7021 gttttgagca gcataaatcc cattaaacat gacattgaga tttccagagg ttggattttt
     7081 aaaaccagta gaaaatcctg ccccacttgc cacaaagcgg tgttgctggt cttcaactga
     7141 acgggcacca actgccatgt aagaaatcaa atcatctaca agcggaaggt tttcaggata
     7201 aagcatttca tcagcagttg tcatccctgt ttctgtgatg acacgatagt gaagatggcg
     7261 aacggctttg attccattga taagactagg cgcttctgtc gcgttaggct ggtgaatcaa
     7321 gcccttatag ccatctccgt tggtacgggg tttggcagta taaacacgca taaccataaa
     7381 gatacgatct gccacttctt cttgtaggac tgccaaacgc ttagcgtatt caaggacagc
     7441 ttcttcgttg tcagatgagc atggcccgat taccaagaga attcgctggt cttctccacg
     7501 tataatggct tctagctctt gatctcgctg tgatttcctc tccaaagcct gaccttctaa
     7561 ttttgacaag gcacgaactt cttctatatt tattttagag ctttttgctg taaataccat
     7621 aactcatctc cttataaagc aaacggatac ccagtaaaaa tttacttttc acaagatatc
     7681 cgcctctaaa ctatctcatt ttattgtctt ttaccgtgac agtttttaaa tttcttacca
     7741 gaaccacatg ggcaaagttc attgcgtcca atctggctca aatccaaatc ttctggcata
     7801 cttgcttggt gagcagcgat attgcgagtc gctgttgtac tgatatgacg ttctgcctgt
     7861 ggtctttctt gttcatgaat ttgtgctttc atcatcaagc gtgtcacatc aaactcaatc
     7921 gaaccaatca tatcattaaa catacggaaa ccttctgcct gatactcaac aacagggttg
     7981 ttctgagcat agccacgaag tccaaccgcg ttacgcaatt gatcaagggc atcgatatga
     8041 tctgtccact tgttatccac cactcgtaga atcaaaactt tttggaattc tttaactgct
     8101 tcttcatcgc gtagttttga aacctgacta tcgtaaacct tcaaggcacg ttggaaaagc
     8161 tcttccttga tggccttatc agacaagcct gacaagtctt ccatcgtaat agaatcttca
     8221 ggaagcaagt tgtacttagc gaagttcaaa attgcctcta gtttctcatc ttgtttagca
     8281 cgcgcatgac cgtcaacgac acgaccaatc gtgcgtttga tcattgcctg aatttcaggt
     8341 gccaagtcac ggtctgcagt aatgacatca taacgttgag tatagataat ctcacgttgt
     8401 tcacgcatga catcgtcgta ttgaaggact tgtttacggg tatcgtagtt atttccttcg
     8461 acacgtttct gagctgcttc aacctgacgc gtcaacatgc gagactcaat ggcctcttca
     8521 gacatgttca agcgttcaaa gattcccttc aagcgttcag aaccaaaacg tttcatcaaa
     8581 tcatcttcaa gagataggta gaattgtgac tcacctggat ctccttgacg acctgaacgt
     8641 ccacgaagct ggttatcgat acgacgactt tcatgacgtt ctgtaccaat aacacaaagt
     8701 cctccaagtt cacgaacacc ttcaccaagc ttgatgtcgg taccacgacc cgccatgttg
     8761 gttgcgatgg taacggcacc acgttgacca gcattcatga tgatttgggc ttctctatag
     8821 tggtttttgg cattcaagac ttcgtgagga acaccagctg caaccaattt cttagaaatg
     8881 tagtcactag tttcaaccgc tactgtacca accaagacag gttgaccctt ttggtaacga
     8941 gccttaacgt cttcgacaac cgctttaaac ttagattcga tacttgcata aagaaggtct
     9001 gagtggtcaa tacgttgaac aggacggttt gttgggattg gaataacacg aatgttgtag
     9061 atttcacgaa attcttcttc ctcagtctta cctgtacccg tcataccaga caattttttg
     9121 tacatacgga aaaggttttg gtacgtgatt gaggcagatg tcttggtttc atcctggatt
     9181 ggcacacctt ctttggcttc aatagcttgg tgcaatccat cagaataacg acgaccttcc
     9241 atggtacgac ctgtaaattg gtcgacaatc aagatttctt gctcttcgct caccacatag
     9301 tcaatatcga gaagcatgat gtagttggca cgaagggcgt tatcgataaa gtgggtcaaa
     9361 gccacgtttt cgatgtcata gaggttttca agtttgaagt agctttcagc cctgtcaatc
     9421 cctgaatcag acaaaccaat agtcttagac tgcacatcga tgatgtagtc atctttgttc
     9481 aaagatttta catagtggtc tgccatgtga tacaactgac tggtttcaac cgcattggca
     9541 cctgatacaa tcaaaggtgt acgagcctcg tcaatcaaga tagagtcaac ctcatcgacc
     9601 aaggcatagt taagcggacg ttgtaccatg ttttcagcgc gaacgaccat gttgtcacga
     9661 aggtagtcaa atccgatttc tgagttagtt gagtaagtaa tatcacactc ataggcttct
     9721 tttttctcca ttggagattt ggtagccaag ttaatcccta ctgacaaacc aagccaagag
     9781 tacaattcac ccatctcagt cgcgtcacgt tctgacaggt attcattaac cgtaactacg
     9841 tgaacccctt tacctgaaag ggcattgagg tataccggca tggtcgcagt caaggttttc
     9901 ccttcccctg tacgcatctc tggcacgtca ccatggtgaa gaacaatccc ccccatgacc
     9961 tgaaccttat aagggaagag acctaggaca cgtttggcac cttcacggac aaccgcaaat
    10021 gcttcgtaaa gcaatgaatc cagtgattct ccattttgat aacgttcctt aaattcaact
    10081 gtttttgctt ttagttggtc gtcagtcaaa gcagccattt ggtcttcgta tttgaaaacc
    10141 ttgtcagcca tcttttccag acgacggatt tctcctttat cattttcgat aattgttttt
    10201 aaaatattag ccatgttttt ccttacttta aattccgaat attttagaat gttcctttaa
    10261 ttttagcaca attactgatt attttcaagg aagaatcccc attttgaaaa ggaaaaagag
    10321 gttagttttc aagctaacct ctccgacttt tatgactgtt taattgccaa aaattggcaa
    10381 aatggcaaat gtgataaata agttaatcca aagagaaaga cagcaaatca gtccaaaaac
    10441 aaagagactg actttcttgg acagcaaggc catcaaaatg gacagaattc caaaaactag
    10501 caagactttc tgtatgatga agatgtcaat ccttaccccc agaatcgctc tatcccaagg
    10561 gataaagaag aaagacagcc agatcagcag aactaaacca atataaacct tggaatagcg
    10621 tgtaataaat gattttagat gtgccataag ccatctccta ggattgtata tcctttacat
    10681 attgtatatg tccattttcg acaactaata catctgtaca aaggtctgaa atatcttccg
    10741 atatgtgcga cgtcaggata accagaccat tttctttttt caggtaagat aaaatgacct
    10801 gtttggttaa cctcacactt ttctcatcca aagcattcat aggttcatcg agaaagagta
    10861 tagaaggaga ggaaataaag gcttgaatca aggccatttt ttgctttgtt cccaaggata
    10921 aatgacggta ttcaacgtct tcaaattcct gtaaatcata gtattgaatc caataggcaa
    10981 ttcttttttc cgtaacttta gatgaaaaat accttaacag ttccaaattt tctctcaggg
    11041 ataaatgaga taaaaactcg actttttcaa ttaaaattcc tgcatcctga atataatgat
    11101 tttttacccc gtaaacttta ccatcttgaa gaacttttcc tttgtcaagc ttaatataac
    11161 cagcaagtat ctttaaaaga accgtcttgc cagatccatt atcaccttta agtccataaa
    11221 tttttcctga ttgaaacacc aaattcaact gatttaaaat aggtcttttc ccataaattt
    11281 tctgtccatt tataatttca agtctcatcg tttatccttt cttaaatttc tcttccaagc
    11341 gactaagaat caacataact aataagctta agagtactaa atacactgtt acaatatgat
    11401 ccatcatcca ttcttgtccc acaaatgcca aaaaatagag aaaaaaagaa aaaccagcgc
    11461 ccgcactaat cagtgctatc agataagaca gtagtaagta caacatcatt aactgagcaa
    11521 gtaaaaagaa aagtgcgact gttttgaaat gaattcctag cggaagtaaa ccaagaaacc
    11581 agacaaacaa atcttgagta aaaaatttag aaatgacaac caatcgtttc cttgtataat
    11641 cgaaaaagga gcttgattga tagcgaatca agtgatggta atggtctgcc aaatcacttt
    11701 caagtaaaat gacagagagc catcctacca tgaaaagcga aaggtttaac aagagtttgg
    11761 ggatttctaa attcgcttca gaatagtcat tataggaaaa aattgaaatc aattctctct
    11821 tgaaaaagat aacagcatcc tgcttttgaa ccgctgttga caatagatag tttatcaaaa
    11881 tcatcatcag aactatacga agtagtaaaa cgatctttct ctttagccaa atattgttaa
    11941 aattcttaaa cattatcttc atatttttta agtaccatat aagggacaac ataaaggata
    12001 taaggagtaa agagtttcat caaacttaca tccccatagc tagcttgcat caaactcgtc
    12061 ataggaacca tatagtaagg caaggcacta tagagcagca tagaaccaag ccaaagaaac
    12121 atcaaatagg tgattgccga acgagtcaca ttccccacat aatcaactat ttgtaaaaaa
    12181 tagattgcat tgatgaaaat accgattaat aggacacaag taaagaacaa atagcttttt
    12241 atctctccat ctaggagtct ttgtaaacca ctcccatcag aaaatagaga attccattca
    12301 agaggagaaa aagtcccaaa gaaataggtt ataattgcaa ttatcagcac agtcactaaa
    12361 tatatcaaac aagggatact tgcaacttgc aaagtcaact ttctttttaa cccttgatag
    12421 ctcacttctc ttccaatact caatcgtaaa actttatttt tcagctcaat atattgaaaa
    12481 cctagcaaaa caatgatgat tggaataata aatgattgat aaaaacctag attgaactgg
    12541 agaatacgaa aatctaatgc tgattttaat aagtcatttt taattgcttg tccatcgaat
    12601 ttagctagat cgggcgcatg aatatcaaaa aagaaataag tcatcaaaac atcacgataa
    12661 accaaagcaa gaaaaagcaa agtaagcaag acataaacaa atttatgcct tgcaaaagtt
    12721 aagagaggaa tcttcataaa ttatacctac tttacgaatt aatttggtga ccaactacct
    12781 gaaatggcag ctgataaatc ccaccaattt tgtcttgcca tatctactgc ataaacatag
    12841 ccaaccgttg cccaattacc atgagttgaa cgagtaccac aggttgttaa cgattctcca
    12901 ctacgagctt ctccattaga atttactata cgatgcttaa cagtcatggg atgtaaattt
    12961 ctacaaggat ttaagttcat gatatattcc gcaccttcat tttctttagt caaataacct
    13021 gttctcttat aactggtagt aataacaaaa ttactaaaag cacctatctg atttgcttct
    13081 acgaaagtag ttccaacagc acttatcatt aaaacaggaa taaccatttt caataatttc
    13141 ttaattttca ttttttctgt ttcctttcat tttttattca agaagattcc ttataacttc
    13201 tattttaaat atagacttat tctaaacgat ggtatatcat acaaattcct cctttactta
    13261 ttatgtatag actctaattt ctttatcccc aaaaccatgt ttcaaaccag ataagttgaa
    13321 tccaactcca aatcaaaaat ccagagtttt tcggtaaatt cttcaaatat tttttaaagc
    13381 ccatgacttt ttccctcctt tttgatataa taataccacc tttttctacg ttttttataa
    13441 atgtagaaaa tattcacatt tacctaggag gaacaatatg agatgggatt atggacaaat
    13501 ttttaaagaa attcgaaagt caaaaggatt gacccaacaa gatgtatgtg gacaagtcat
    13561 acatcggaca actctaacaa atattgaaca cggtaaagtt attcccagtt ttgaaaacat
    13621 ggtatttctt cttgaacaaa ttgatatgag cttggcagaa ttcaagtata tatgcaacga
    13681 ataccatcct agtaaaaggc gagatattat tgtagagagc caaaatccgt ctacttttca
    13741 agatactaga aaaatggttg aactcactga gaaatgtcaa aaatatctta agacacatca
    13801 cgatgttcct attcaaaata tctatcgtca tacaaaaatt gtcacagagt tacgaactaa
    13861 aggattcaaa aacaaccacg tcttgaaaga tttgtctgaa gaaatttggg actatcttga
    13921 acctatggat acatggtaca ttagtgattt gaaattgctt ggaaccattc tctttttctt
    13981 tccttctgaa aatcttcccc ttcttattga tagaattatg aaaaccatcg agaaatataa
    14041 atacttccga gaaacaaaag tatttttatc atctttttta gccaatctct ccactgtata
    14101 ttttcaacat catttattca aagaatgtga aacaatcacc ctacaacttt tggtattagc
    14161 agaagaatta aaaatatacg atattcttgg attctctcaa gtacgcttgg gaatactaca
    14221 acataactcc gatttgattg ataaaggaat aactttatta cgactaacca aagaagaggc
    14281 attagttaaa atactcgaaa aagaaattaa tgatttttca aaccta
//
LOCUS       DAVMAF010000042        14163 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMAF010000042 DAVMAF010000000
VERSION     DAVMAF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.042"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            71..868
                     /locus_tag="VYO97_001351"
     CDS             71..868
                     /locus_tag="VYO97_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1754137.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGFLMGLPYAFLNKNEDTYVD"
     gene            1016..1138
                     /locus_tag="VYO97_001352"
     CDS             1016..1138
                     /locus_tag="VYO97_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV1754138.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1160..1681
                     /locus_tag="VYO97_001353"
     CDS             1160..1681
                     /locus_tag="VYO97_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1754139.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFD"
     gene            1682..2275
                     /locus_tag="VYO97_001354"
     CDS             1682..2275
                     /locus_tag="VYO97_001354"
                     /inference="COORDINATES: protein motif:HMM:NF013237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1754140.1"
                     /translation="MTSGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ASSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALA
                     EKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKSLVVHPATITHGQLS
                     EKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI"
     gene            2371..3645
                     /locus_tag="VYO97_001355"
     CDS             2371..3645
                     /locus_tag="VYO97_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1754141.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3655..4533
                     /gene="truB"
                     /locus_tag="VYO97_001356"
     CDS             3655..4533
                     /gene="truB"
                     /locus_tag="VYO97_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1754142.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4774..5076
                     /locus_tag="VYO97_001357"
     CDS             4774..5076
                     /locus_tag="VYO97_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1754143.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5184..5822
                     /gene="udk"
                     /locus_tag="VYO97_001358"
     CDS             5184..5822
                     /gene="udk"
                     /locus_tag="VYO97_001358"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1754144.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5950..7290
                     /locus_tag="VYO97_001359"
     CDS             5950..7290
                     /locus_tag="VYO97_001359"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1754145.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRSTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLDSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7268..7480
                     /locus_tag="VYO97_001360"
     CDS             7268..7480
                     /locus_tag="VYO97_001360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1754146.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7477..8352
                     /locus_tag="VYO97_001361"
     CDS             7477..8352
                     /locus_tag="VYO97_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1754147.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFDEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8345..9160
                     /locus_tag="VYO97_001362"
     CDS             8345..9160
                     /locus_tag="VYO97_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1754148.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9153..9584
                     /locus_tag="VYO97_001363"
     CDS             9153..9584
                     /locus_tag="VYO97_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1754149.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9591..11258
                     /gene="recN"
                     /locus_tag="VYO97_001364"
     CDS             9591..11258
                     /gene="recN"
                     /locus_tag="VYO97_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1754150.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11260..11988
                     /locus_tag="VYO97_001365"
     CDS             11260..11988
                     /locus_tag="VYO97_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1754151.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12067..13890
                     /gene="lepA"
                     /locus_tag="VYO97_001366"
     CDS             12067..13890
                     /gene="lepA"
                     /locus_tag="VYO97_001366"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1754152.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13971..>14163)
                     /locus_tag="VYO97_001367"
     CDS             complement(13971..>14163)
                     /locus_tag="VYO97_001367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754153.1"
                     /translation="IKEQTRKLAAGYSKHSFEVVDETDEVSSKHDFEVVDETDEVSNH
                     TYGKATLTWFEEIFEEYKN"
BASE COUNT         4185 a         2593 c         3361 g         4024 t
ORIGIN      
        1 tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa aaaagattta
       61 ggagtttatt atggtttctt cagaatttat ttcaaagatt gaatttgctt gcaataagaa
      121 agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt tcgcaggtgc
      181 atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta ataaaattgc
      241 accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg cctacattgt
      301 ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg gtagtttctt
      361 aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct tgttcaacct
      421 tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg cgaatctgac
      481 acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa atgaattggt
      541 cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt catttatttt
      601 ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca tgtttgtatt
      661 cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga aattcagtgt
      721 tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact ggggtgtgac
      781 tttcatcgga aactttatcg gaggaggctt cttgatgggt cttccatatg ccttcctcaa
      841 taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg tgcttttttc
      901 attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact gatatttgta
      961 aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc accttatgac
     1021 ttgtgatttt aaatctgaaa ctctacaact acatgctggt caagttgtgg ctccagctac
     1081 taagtctcgt gcagtgccga tttatcaaac aacatttttt gtttttgatg acacgtagga
     1141 aggtgccgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta tcaccaatcc
     1201 tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag gtatgactgc
     1261 agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg ctgcttcgac
     1321 tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg gtatcacaac
     1381 aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag acaataccaa
     1441 gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc tggaaaaact
     1501 ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt ttgcaatacc
     1561 ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg cgactaagtt
     1621 tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc gttttgactg
     1681 aatgacttca gggaaattcc ctcaatttgt tgacgagggt tcaagctgcc acaatttgag
     1741 ctatactcgt gatgtgggtg cagtagcctt tattatagct gttcgagttc aattgcttcg
     1801 tgatacaggt gcagcctcgt caccattcaa tgctttcctc ttgctacaaa gacttgaaac
     1861 cctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg attttcttgt
     1921 caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc cttattatgc
     1981 cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc acgtcaaagg
     2041 tgacgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg accttgcaaa
     2101 cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca atcactcacg gtcaattgtc
     2161 agaaaaagac ctagaagcag caggtgtcac accaaatcaa atccacttgt ctatcggtct
     2221 tgaaaatgta gaagatttga ttgaagactt acgcttggcc ttggaaaaaa tttaaagtaa
     2281 aagaagataa acagtgggtt ttgactcact gtttttgatt ttccctcagg catgatataa
     2341 tggttacaga agtctagaaa gaggaatgta atgaacgaaa tcaaatgtcc caactgtggg
     2401 gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt gagaacggca
     2461 gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc tgagcaaaag
     2521 gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc gcaattgcag
     2581 agtcaaatcc aaaactttga tacagaaaaa gaattggcca agaaagaagt tgaacagaca
     2641 agccatcagg ccttattggc taaggacaag gaagtacagg ccttggaaaa ccagttggcg
     2701 accttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct agaaaaagaa
     2761 cgcaatcagg tcaaaaatca gctcctactg caagaaaagg aaaatgagtt gtctttggct
     2821 tctgttaagc aaaactacga agcccagctc aaagcagcca gtgaacaagt cgagttttat
     2881 aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga acagtatgca
     2941 gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt tgagaaggat
     3001 aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg tgatgaaaat
     3061 ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg aacagagaag
     3121 aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga gaagaactgt
     3181 gagtatgccg ttttggtgac catgcttgag gctgataatg actactttaa cacagggatt
     3241 gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt ctttatccaa
     3301 ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca ggagttggcc
     3361 ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga tgcctttaag
     3421 ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc tattgatgaa
     3481 atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac atctgaaaac
     3541 caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt gacccggaaa
     3601 aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac
     3661 ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt ttttaaactg
     3721 cgtaagattt tgggaaccaa gaaaattggt catggcggaa ccttggatcc ggatgtggtg
     3781 ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat gcaggacgag
     3841 ggtaagatct atgaggggga aatcactctg ggctattcca cgacgactga ggatgctagt
     3901 ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaagct tgttgatgaa
     3961 gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc ggcagttaag
     4021 gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa
     4081 cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga tggccaactt
     4141 gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac tttgtcagtt
     4201 gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg tactagtgct
     4261 gctggcttac aattagaaga tgctcttacc ttggaggaaa ttgctgaaaa agtagaggct
     4321 gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt caaagttttc
     4381 ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct agaccaaacg
     4441 gacaaagaac tggctgcctt tgaagatgat aaattattag ccattctaga aaaacggggc
     4501 aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa atcgggtgat
     4561 agataacaat tgcttgataa aaccccatac taatagtaga atggttttgg gaattataat
     4621 attccaattg ttgcgagttg taggtactca aataatctat atagaaattt agaggtgtga
     4681 aatgaagcaa tttaaaattc tttcagataa atatttagag tccattacag gttctggtgg
     4741 gaacttaggc ccaggatttg gtgtgataat tccatgatgc gaaatgagtt tcgagaaagg
     4801 gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag tcacctgttt
     4861 cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aaacggtcat ggccaatttg
     4921 agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa gtctgtcatt
     4981 gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact ggctttcttg
     5041 cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc agagggaaga
     5101 ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata gaatagacga
     5161 cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga gtgacaggtg
     5221 gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt cctgatgaaa
     5281 agatttccat gattgagcat gattcatact acaaggatca gtctcatttg acctttgaag
     5341 agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg atgatcgagc
     5401 agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac tatacagagc
     5461 atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc gttgagggca
     5521 ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt tttgtggata
     5581 cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggaa cgtggccgta
     5641 gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac caccagttta
     5701 tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc aataccgtgg
     5761 ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga aacagcaaat
     5821 aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg tacataaatg
     5881 gagattttta ggcatatttt tggtataata atacccataa aagagcaagt aaatgaatag
     5941 taggtggaga tggaaaagta tttatcggta acaactttga ccaagtatct gaaaatgaaa
     6001 ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc caactttcgt
     6061 aaacgatcta ctcaccaata tttctcccta aaggatgacc atgcagttat tcaagcgacc
     6121 atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat gaagatcaat
     6181 gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat cattgaaaaa
     6241 gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa gaaaaaattg
     6301 acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt ttctaagaga
     6361 attggggtag taaccagccg tagtggagcc gttattcgag atattatcac gaccgtcagc
     6421 aggcgattcc caggtgttga catccttctt tatccgacca aggttcaagg tgaaggggct
     6481 gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt ggatttgctc
     6541 attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga agaaattgtg
     6601 gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca tgagacggat
     6661 gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc ggcggctgaa
     6721 ctggcaacac ctgtgaccaa gttggatgta ttaactcatt tgcaaaatca ggaaaaacgg
     6781 atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa aaaatgcagt
     6841 cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca acgcttggac
     6901 caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa caaacaatta
     6961 gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat ccaacgctat
     7021 caagaccgtt taggacagtt ggacaagctc ttagatagcc aaatggcgtt agtttatgac
     7081 gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga tactagccga
     7141 atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccattgtaga ttcggttgag
     7201 agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt agaattagag
     7261 gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg aaaccattgt
     7321 ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct ttcaaaaggg
     7381 catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga ccttggtcaa
     7441 ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga aaaattagct
     7501 cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc tagtttacgg
     7561 gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt tctcttgtta
     7621 gaagttctgg aatccttgca ggttaccatc aaacctgctc acgcgcaggt agctactgcc
     7681 ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat ggatgatgac
     7741 gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat ggccattttg
     7801 gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga tttgccaagt
     7861 cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct gggtatggtg
     7921 gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga agaacttcag
     7981 actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc ggcagctatt
     8041 atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga attgattgga
     8101 cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga tgaaatcggc
     8161 aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt gttgggcttg
     8221 gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa attagaagaa
     8281 attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga aagtttgaga
     8341 atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt ttgaaacgag
     8401 agagcaggcc aagcgaggtg tgatggctgg cctagtcgta gcagtcctta atggagaacg
     8461 gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca agggggagaa
     8521 actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg tctttgattt
     8581 gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt ttaccgatgt
     8641 catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca atcagttggc
     8701 ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt ttcgctatgc
     8761 tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga gtttcatttc
     8821 ccttagtctg attttgccag ccttgcaccg tgtcttggct gatcaaggtc aggtggtagc
     8881 acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg gaattattcg
     8941 agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag tagaggcagg
     9001 tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa atattgaatt
     9061 tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg agattaaaga
     9121 agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga cttgaaaaaa
     9181 ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta gaacatttga
     9241 aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa gagttaggta
     9301 ttgtcaaaat tcctttgaga gacaacacct atgtctacga attaccaaaa tcaatcgtaa
     9361 aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat aagatgatca
     9421 atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc gatacttttg
     9481 cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt gccagaagtg
     9541 gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat atgttacttg
     9601 aaatttcgat aaaaaacttt gccattattg aagctatttc cctcaatttt gaaaagggga
     9661 tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat gccatgaata
     9721 tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca aaggcagaga
     9781 ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt gatgagcaag
     9841 gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat ggtcgtagta
     9901 tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgagctatt gggcaacatc
     9961 ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa ctgcatatcc
    10021 agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc tatcaaacga
    10081 gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa aaccaacagg
    10141 aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag gcagcaaact
    10201 tgcaggctgg agaagacttg gctctcaatc aagagcgaga taaactcctc aaccacaaaa
    10261 atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat ttttcaagtc
    10321 tggccaatgt tcgttcagct atgaatgaca tggaaagtgt cgaagagtat gatcctgaat
    10381 accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac attagcaaac
    10441 gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg caggttgaga
    10501 atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt gatgatgttt
    10561 tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat aatctttcgt
    10621 ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat ttggcaggtc
    10681 aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt aaacaagaac
    10741 tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag ggaaaattca
    10801 gtcgtggggg aaatgaaatg gttgaatttt atatttcaac caaccctgga gaagacttta
    10861 aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta gccattaagt
    10921 ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg gatacgggag
    10981 tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt cagtatggtc
    11041 aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat caattcttta
    11101 ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg acagtcgaag
    11161 agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa gcagccctga
    11221 cgcaagccag agaattgttg agaaacaggg agaaataaga tgacagatta ttatgtaatt
    11281 ggagatgttc acggaaaagc tgggatgctg gaagaccttc ttaaaacctg ggatggtcag
    11341 acccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca ccgtgttctt
    11401 gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtctgg aaaccacgag
    11461 tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta tcgtcgcaat
    11521 ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc agttgatgga
    11581 gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt cattcgtcaa
    11641 atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat tgatttgacc
    11701 ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa accattccac
    11761 ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt ctatggtttg
    11821 ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa gattggaatg
    11881 gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga ccaaggaatg
    11941 acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta ctcctagcag
    12001 ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc tgaaaggaag
    12061 agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa cttctctatt
    12121 atcgcccata ttgaccatgg aaaatcaact ctagcagacc gcattttgga aaaaacagag
    12181 acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggagct agagcgggaa
    12241 cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa agatggggaa
    12301 acttatattt tccatttgat tgacacacca gggcacgttg actttaccta tgaagtttca
    12361 cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca aggaattgag
    12421 gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat catgcctatc
    12481 attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga gattgaagat
    12541 gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg tattgggatt
    12601 gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga tgtgacggcg
    12661 ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt tatcctccaa
    12721 gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat gagcaatagt
    12781 aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg tcgtgatttc
    12841 cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca ggatactcgt
    12901 gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca tggttataag
    12961 cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa atacaatgac
    13021 ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt tgaaccagaa
    13081 acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct ccatatggat
    13141 gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac agctccgtct
    13201 gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa cccatctgag
    13261 tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc gcaaatcatg
    13321 gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg tggggacttt
    13381 gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat tcctcttgct
    13441 gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta tgcaagcttt
    13501 gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat ccttctcaat
    13561 ggagataagg tggatgccct cagctttatc gttcacaagg actttgccta cgaacgtggg
    13621 aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga agttccaatc
    13681 caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct tcgtaagaac
    13741 gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct tgaaaaacaa
    13801 aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca agaagccttc
    13861 ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc ttgttcacag
    13921 gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt ttagttttta
    13981 tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt atggttactg
    14041 acttcgtcag tttcatctac aacctcaaaa tcatgttttg agctgacttc gtcagtttca
    14101 tctacaacct caaaactgtg ttttgagtaa cctgcggcta gcttcctagt ttgctctttg
    14161 att
//
LOCUS       DAVMAF010000043        13742 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMAF010000043 DAVMAF010000000
VERSION     DAVMAF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.043"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            40..957
                     /locus_tag="VYO97_001368"
     CDS             40..957
                     /locus_tag="VYO97_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1754154.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            968..1858
                     /locus_tag="VYO97_001369"
     CDS             968..1858
                     /locus_tag="VYO97_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1754155.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            1887..3503
                     /locus_tag="VYO97_001370"
     CDS             1887..3503
                     /locus_tag="VYO97_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1754156.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFTFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            3513..4832
                     /locus_tag="VYO97_001371"
     CDS             3513..4832
                     /locus_tag="VYO97_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1754157.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHMDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(5040..5112)
                     /locus_tag="VYO97_001372"
     tRNA            complement(5040..5112)
                     /locus_tag="VYO97_001372"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5077..5079),aa:Lys,seq:ctt)
     gene            complement(5377..6648)
                     /locus_tag="VYO97_001373"
     CDS             complement(5377..6648)
                     /locus_tag="VYO97_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1754158.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            6918..7157
                     /locus_tag="VYO97_001374"
     CDS             6918..7157
                     /locus_tag="VYO97_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754159.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            7322..7501
                     /locus_tag="VYO97_001375"
     CDS             7322..7501
                     /locus_tag="VYO97_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754160.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7644..7817
                     /locus_tag="VYO97_001376"
     CDS             7644..7817
                     /locus_tag="VYO97_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1754161.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            7854..8306
                     /locus_tag="VYO97_001377"
     CDS             7854..8306
                     /locus_tag="VYO97_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1754162.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8597..9067
                     /locus_tag="VYO97_001378"
     CDS             8597..9067
                     /locus_tag="VYO97_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1754163.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            9086..10174
                     /locus_tag="VYO97_001379"
     CDS             9086..10174
                     /locus_tag="VYO97_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1754164.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            10155..10313
                     /locus_tag="VYO97_001380"
     CDS             10155..10313
                     /locus_tag="VYO97_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV1754165.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            10423..11457
                     /locus_tag="VYO97_001381"
     CDS             10423..11457
                     /locus_tag="VYO97_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1754166.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGVSK
                     SSVSRELSED"
     gene            11519..13702
                     /locus_tag="VYO97_001382"
     CDS             11519..13702
                     /locus_tag="VYO97_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV1754167.1"
                     /translation="MGPITIPKKTIITLEHLKYLHFSLEETKTYWEKNNIIDGILISI
                     YYNRIVAKSNRINGYFNVGGGNPFPNDTIVGAKFNDEKTKHIVTHYISRDALNKTITV
                     LSKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQYLRDSCFIENFGVE
                     HTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLLDEKNIELLLSKAP
                     YLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLFDKRVYFNEWVEYH
                     DFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRHFGVSLQSGFNSFT
                     IIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQFENDVIFIIAGTNA
                     SVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFVKPDVSYYGGGNGD
                     FINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALLIDAAIPWNDKKTF
                     TDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFPVPISSESYPYVAK
                     ATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAEDSPGYVRENAARN
                     IFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSGDGQGVRFGVVVTL
                     KELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE"
BASE COUNT         4376 a         2222 c         2903 g         4241 t
ORIGIN      
        1 ttctagaaaa aaatgaaacg tttcaaaaag gaggttgcta tgaatagcaa agcgaagcaa
       61 gtttctcttt gggaaagaat caagaaacaa aaactcttgt tattgatgac tgtccccggt
      121 ttagttttaa cctttatctt taaatacatc cctatgtatg gggttttaat cgcatttaaa
      181 gattacaatc ctttaaaagg aattttaggg agtgattgga ttggtttttc tgagtttaca
      241 aaattcatat cctctcccaa ctttggtatc ttgttagcca acacattaaa attaagtatc
      301 tatggtttat tgcttggctt tttaccacca atcattcttg ctattatgct caatcaactc
      361 ttgagtgaaa aagtcaaaaa acgaattcag ctcattttat acgcaccaaa ctttatctca
      421 gtcgttgtta ttgtcggtat gattttcctc ttcttttcag tgggaggacc aatcaacaat
      481 tttctttcta tgtttggaat gaaggctgac ttcttgacaa atccagactt ctttagaccc
      541 ttatacatct ttagtggtat ctggcaagga atgggctggg cttcaacgct ctacacggca
      601 acattggtaa atgtagatcc agccttagta gaagcagccc gactggatgg agccaatatc
      661 ttccaacgaa tctggcacat tgatattcca gctcttaagc ctattatggt tatccaattt
      721 gttttagctg caggtggaat tatgaatgtc ggatatgaaa aagcattctt gatgcagaca
      781 tcgttaaatt tgccaacttc tgaaattatc tcgacatatg tctataaagt tggtcttgta
      841 tcaggagact attcttactc aacagcggtt ggtttgttta atgcagtgat taacgtagta
      901 ttgcttgttg cagttaacca aatcgttaaa cgcatgaata atggtgaagg aatttaagga
      961 gggaagtatg aaaaattcga ttatggatac aaaatttgat agacgtatct tactcttaaa
     1021 taaaatcatt attgtcttta tcgttttgat gactttgctt cctttacttt atatcgtcgt
     1081 agcatccttt atggatccta aggttctggt tagtagaggg attagcttta atccagccga
     1141 ttggactgta gaaggttatc agcgtgtatt cagtgaccaa tctattctaa gaggttttat
     1201 caattctcta ctatactctt ttggatttgc agctttaaca gtcttgctat ctgtgtttac
     1261 agcttatcct ctttctaaga aagacttggt tggacgtcgt tggattaact acttcttgat
     1321 tgtaactatg ttctttggtg gtggtttagt cccaacttac ttgctcgtaa aagaattggg
     1381 aatgctcaat actccatggg caatcattgt tccaggtgct gtcaatgttt ggaatattat
     1441 tcttgctagg gcctatttcc aaggattgcc tgaagaatta gttgaagctg ctgtcattga
     1501 tggtgcaaat gatttacaga ttttcttcaa aatcatgctt cctcttgcaa aaccaattat
     1561 gtttgttctc ttcctctatg cttttgtagg acagtggaac tcatactttg atgcaatgat
     1621 ttatatcaag gatccaaact tggaaccatt gcaacttgta cttcgtaaaa ttctcattca
     1681 gagccaacca ggtcaagaca tgattggagc acaagcggct atgaatgaaa tgaaacgttt
     1741 agctgaattg attaaatacg caactattgt catttccagc ttgccattga ttgttatgta
     1801 tccattcttc caaaaatact ttgataaagg aattatggct ggttcactta aaggataaaa
     1861 aaagaaaaaa taaaaggagt tttctcatga aattcaaaac attctcaaaa tcagcagttt
     1921 tgttgacagc tagtttagca gtacttgcag cctgtggctc aaaaaataca gcttcaagtc
     1981 cagattataa gttggaaggt gtaacattcc cgcttcaaga aaagaaaaca ttgaagttta
     2041 tgacagccag ttcaccgtta tctcctaaag acccaaatga aaagttaatt ttacaacgtt
     2101 tggagaagga aactggcgtt catattgact ggaccaacta ccaatccgac tttgcagaaa
     2161 aacgtaactt ggatatttct agtggtgatt taccagatgc tatccacaac gacggagctt
     2221 cagatgtgga cttgatgaac tgggctaaaa aaggtgttat tattccagtt gaagatttga
     2281 ttgataaata catgccaaat cttaagaaaa ttttggatga gaaaccagag tacaaggcct
     2341 tgatgacagc acctgatggg cacatttact cattcccatg gattgaagag cttggagatg
     2401 gtaaagagtc tattcacagc gtcaacgata tggcttggat taacaaagat tggcttaaga
     2461 aacttggtct tgaaatgcca aaaactactg atgatttgat taaagtccta gaagctttca
     2521 aaaacgggga tccaaatgga aatggagagg ctgatgaaat tccatttaca tttattagtg
     2581 gtaacggaaa cgaagatttt aaattcctat ttgctgcatt tggtataggg gataacgatg
     2641 atcatttagt agtaggaaat gatggcaaag ttgacttcac agcagataac gataactata
     2701 aagaaggtgt caaatttatc cgtcaattgc aagaaaaagg cttgattgat aaagaagctt
     2761 tcgaacatga ttggaatagt tacattgcta aaggtcatga tcagaaattt ggtgtttact
     2821 ttacatggga taagaataat gttactggaa gtaacgaaag ttatgatgtt ttaccagtac
     2881 ttgctggacc aagtggtcaa aaacacgtag ctcgtacaaa cggtatggga tttgcacgtg
     2941 acaagatggt tattaccagt gtaaacaaaa acctagaatt gacagctaaa tggattgatg
     3001 cacaatacgc tccactccaa tctgtgcaaa ataactgggg aacttatgga gatgacaaac
     3061 aacaaaacat ctttgaattg gatcaagcgt caaatagtct aaaacactta ccactaaacg
     3121 gaactgcacc agcagaactt cgtcaaaaga ctgaagtagg aggaccacta gctattctag
     3181 attcatacta tggtaaagtg acaaccatgc cagatgatgc caaatggcgt ttggatctta
     3241 tcaaagaata ttatgttcct tacatgagca atgacaataa ctatccaaga gtctttatga
     3301 cacaggaaga tttggacaag attgcccata tcgaggcaga tatgaatgac tatatctacc
     3361 gtaaacgtgc tgaatggatt gtaaatggca atattgatac tgagtgggat gattacaaga
     3421 aagaacttga aaaatacgga ctttctgatt acctcgctat taaacaaaaa tactacgacc
     3481 aataccaagc aaacaaaaac tagaggttga ttatgggaga taagaaatac acagtagaaa
     3541 aagccaatcg ttttatagca gaaaataaac atctcgttaa tactcaatat aagcctgaag
     3601 aacatttttc agctgagatt ggttggatca atgatccaaa tggatttgtt tattttcgtg
     3661 gagaatacca tctcttttat caattctatc catatgatag tgtttgggga cctatgcact
     3721 ggggacatgc taaaagtaag gacttggtga cttgggagca cttgccagtg gcacttgctc
     3781 ctgaccaaga ttatgaccga aatggttgtt tctcaggctc tgccattgtc aaggatgatc
     3841 gcctctggct catgtacact gggcatatcg aagaaaaaac cggtgtccgc caagtgcaaa
     3901 atatggcatt ttcagatgac gggattcact ttgaaaagat ttcccaaaat ccagttgtaa
     3961 ctggaacaga cttgccagat gagttgattg ctgctgattt ccgtgatcca aaactctttg
     4021 aaaaagatgg acgctattac tccgtagtag ctgccaaaca catggataat gtgggctgta
     4081 tcgttctact agggtccgat aacctagtag aatggcagtt cgaatccatc tttttaaaag
     4141 ggggagaaca ccaaggcttt atgtgggaat gcccagatta cttcgagtta gatgggaaag
     4201 attgccttat tatgtcaccc atgcgttatc agcgtgaggg agactcatat cataacatca
     4261 actcatcgct tttgttcacg ggtaaggtag attggagaga aaaacgtttt atcccagaat
     4321 cagttcaaga aattgatcat ggccaagact tctatgcgcc tcaaacattg ttggacgatc
     4381 aaaatcgtcg tatcctgatt gcttggatgc agacatgggg gcgtaccctt ccaacccatg
     4441 accaagaaca caaatgggca tgtgccatga ctttacctag aattctaaga ttggaagatg
     4501 gcaaactaag acaattccct gttaaaaaag gccaatatca aatccaaata gataaagatt
     4561 gtcattacca cttaggaaat gatacagatt atcttgaatt tggttatgac agtaatgcgc
     4621 agcaagttta cattgatcgt agccatctta ttcaaaaaat tctaggtgaa gaagaacagg
     4681 acactagtcg acggtatgta gatattgaag ctaaagaatt ggaagttgtt ctagataaaa
     4741 attccatcga gatttttgtc aatcaaggtg aagcaagctt gactgcaact tattacttaa
     4801 cggtgccagc tgagctatca cgaattgatt aaaaattaag ttatttctcc taaagaaaaa
     4861 gttctctttc taaaatagtg gaaagaggac tttttgtgtt ttgggtatat aagcttagtt
     4921 tatggtattt gtaaaattgg tgttggatta tgatttaagc tagttttcta aagaatttga
     4981 aaaaaatttt atttaagcaa aaaaaccttg gttccaaggc ttttcctgtt gtatttatat
     5041 gccccctaca gggatcgaac ctgtgaccca cggattaaga gtccgctgct ctgccagctg
     5101 agctaaggag gcaaagaaaa aagctgtatt ggtgccgaaa cttcacggtt tgtattgaac
     5161 ccgcgcaatt aagcaggtgg gcaactcgct ctaactgaag ctgtttccgt gtgagacggc
     5221 ctacatgctg ttagaagact tttgtttccc taataataca aaaaatagtc ggtcaacact
     5281 taagtgtgaa gtcgtacacc acagcgtttc tatatttata tgataccact tttttaaaaa
     5341 aaatcaagag gaaagtgcaa ttttttgaaa aggatttcag atttttcgca aatggtcgat
     5401 agcttccttt ctttgagcca aagcccgttc atatttacca gtatcttctg cttggaaata
     5461 gtgatgatta cgaatttttt ctggcagata gtcttgcttt acccaatttc caggatagtt
     5521 gtgtggatag agatagtctt gggcattccc cagttccttg cttccactgt agtgcccatc
     5581 acgtaggtgt cgcggaatag gcaagtgccc tgatgttttg aggtcagcaa gtgccttatc
     5641 catagctaca taggctgagt tggattttgg agaaagggcc aaatcaatca cgacattggc
     5701 aatgagaatg cgggcttctg ggaacccaat cttctgggca gcatccagag cagtcacggt
     5761 atgaatctgg gcctcaggat tggctaagcc gatatcttca taggcgataa cagtcaagcg
     5821 acgagcgaga ctaggcagat ccccagcctc aatcaagcgg gcagcatagt ggagactggc
     5881 atccacatct gagccacgga tagacttttg cagggcagat agaacatcat agtgaccgtc
     5941 tccatccttg tccatagtga tgtagcttct ctgaagacta ttttccatga tgtctaaggt
     6001 gatgtggcga atgccctcgt cattctcagg ggtagagaga acagccaagt ccagtgagtt
     6061 aaaggcagag cgaaggtctc cgtttgtaga ggttgcgatg aaatccagcg catcctcatc
     6121 tagttctatt ggaaaatcaa aaccacgttc agggttactt agagctatct gcagggcctc
     6181 tttgacgtct tggttagaca gaggttccaa ctcgaaaatt tgaacacggc tacgaatggc
     6241 aggagtgaca gagaagaaag gattttcagt cgttgctcca atcatgatga ccagtccact
     6301 ttccaagaga ggcaagagga agtcttgctt ggtcttatct agtcgatgaa tttcgtctag
     6361 caataggacg agaccaccag aaaatttagc ttcttccgag atttcttgca gtcgcttttt
     6421 actatcaact gtcgcattga aagttcgaaa ggcatacttg gtcgttccag cgatggcaga
     6481 ggcaatactg gttttgccga ttcccggagg gccatataga atcatggagg acaggcggtt
     6541 ggcttccacc atgcggcgga tgatttttcc aggtccgacc agatgctcct gaccgatgac
     6601 ctggtcgatg gttttagggc gcatgcgaag cgcgagattg tctggcatag cagtcctttc
     6661 taacgtggat ttgagtagaa tgactcatct actatatctt actatgtcca ttctagccta
     6721 ttagttgtga tatttcaatg gttttgtaat aatcagggag tgagatagaa tcttttttac
     6781 tatttaattt aatgaggaga ggtatcagca tgagagattt gattttcctt aaagctcaaa
     6841 ataggatagt tgagcctaaa gaagtggaag tgctgacttg agaaataata ttgctgaatg
     6901 tcattgtatg aactataatg aaaagagagg tgataagcaa tggaaatgat ggaccaagtc
     6961 aggaaatttt gatttttaca aaacaaattc gtcattggat tcttagtgat caagttattt
     7021 cagggaaaag aaaacttttc ttccgggaag atactccaaa agagatttta gatttgtacg
     7081 aaaacattaa atctaaactt gattttgctt accaagaagt tcattctaat aatgggttga
     7141 aaaaatatga aaaatagaat aattgatgtt tttgaagtag taaatcgcct ccttgtaata
     7201 actgttgaaa atcctgattt cgaagacttg agagttaatc atactcaatg aaaatcaaag
     7261 agcaaactag gaaactagcc gcaggctgta cttgagtacg gtaaggcgac gttgacgtgg
     7321 tttgaatttg attttcgaag agtatcagtt tgtaaaaatt ggagataaaa aatatagagt
     7381 acgcagtggt cctatgattc attcaactcc gcctcaatct gtcttagatc gagatacttt
     7441 tacaattgac tatacagatg atgaattgct cgataaagaa gcagttttta ctactcatta
     7501 gaaaaatagg tctgaataaa ttggtttccc ttagaaccac atctaggggc ttttatgtat
     7561 aatagaataa taagtatcac tcctttacaa tacgtgtaac acgtgttgta atggtgttgt
     7621 caggaggtaa aagcgctata cctatgacac aaaaagagat ggttaaactc cttacggccc
     7681 atggttggat aaagactaga ggcggtaaag gctcccatat taaaatggaa aagcaagggg
     7741 aaagacctat cacaatcctt catggtgagc tgaataagta cactgaaaga gggataggaa
     7801 agcaagctgg gttgtaaaat tcagtccctg ctttttgata tggaggtagt gaaatgttag
     7861 ttacgtatcc agctttattt tattatgacg acacagacgg aacagaagcg acttattttg
     7921 tccatttccc agattttgaa tactcagcta cacaaggaga ggggatttct gaggctttgg
     7981 ctatggggtc ggagtggcta gggataactg ttgcagattt gatagagagt gatggagagt
     8041 tacctcagcc atcagatatc aatagcttgt ctttaattga taatgatcct tttaaagatg
     8101 atgaagattt cgtgtcaacc tatgaccttg ataaatcttt tatttctatg gtatctgttg
     8161 atgtatcaga atacctagga agtcaggaac ccattaaaaa gactttgacc ataccaaaat
     8221 gggcagataa gttgggacga gaaatgggac ttaacttttc tcagactttg acagacgcta
     8281 ttgcagataa gaaagttcaa gcctaagatt aagcgtggta tcgtggtaat agtcaataaa
     8341 aaagcacgtc cacttgtgct agttactcgc ctattgattt tatagattta ttagcccttt
     8401 cgagggcttt ttatattgat ttttatgaga aatgaagaaa tcagactttt aagaaaatca
     8461 ttgataccaa gggtttaaat gaggtaatat ggtataatta ggacataaaa taattttgtg
     8521 gtaagatggt agtatctatt ttagcatatt tccgagcaat cggggcgatt aaagagtcgc
     8581 atagaaagag gacaaaatgg caacatacgg atttttagat attttagagg aagagttgga
     8641 caagaacttt ccctttgact ttgagattag ttgggacaag cgtaaccacg cggtagaagt
     8701 gagttttcta ttggaagcgc aaaatgctgc aggtgtggag atggtggatg aagacggaga
     8761 ggttttatca gatgacattc tttttgagga agcggtcctt ttctacaatc ctgccaagtc
     8821 aacagtcaat gaggaagact atttgactgt tattccttac ctgcctaaaa aaggattttc
     8881 ccgtgaattt ttagcttatt ttgccctttt ccttaaagat actgctgagg ttggactaga
     8941 taccctcatg gactttttgg aagatccaga agcagaggaa tttgttatgg aatggaatca
     9001 agaagtcttt gaagaaggaa aagttggctt ggaagaggga gaattttatc cttatccaag
     9061 atactaggag ttggttggag gtgttatgaa gaaaattggg acttatttgg tttatgtgtt
     9121 agctgttgtc tttattatgc tagcttttgc ttgtggaaca atcgcatttg cagagttggg
     9181 gtattccgca gttttagcct ttacttttgg ttatgccttc gctcttctaa gcatgtattt
     9241 aatctttatt cttcatgagc tgggtcatgc tttttgtggc tacttgacag gctatcggct
     9301 ggtggctttt ggattaggac attttatttt gaccaaaaag tcaggcaggt ttcatcttag
     9361 tagaacagcc attctgaaaa atgttggtgc tcaatacatt ggtttaaaag aggatgaaag
     9421 cgatcaaaga atcatcctga tgctttcagg aggcttgatg gttcatctcg gcttgatatt
     9481 attggcgata gtgtttggat ttttgacaag aagctggtat tttgcaggga cttggatttt
     9541 tcttaattta tctttcttcc taaataacat tttgccagtc gacatcaccg atggagcaaa
     9601 aatttgggaa ttactacaac accctgaaaa taccaaatac gcctacctgg tattgagaca
     9661 ttctgctcaa actttgctgg ctcctcaaga atatgatttg aaagactttg ttcaagcagt
     9721 tcctgaggat gcacgaggga gttttgcgga tggtgtatta ggtatgcaag gtgaagtttc
     9781 cattttggaa gggaaggaag aagtagctaa acagcagttt caagcattac ttgagaggac
     9841 aactactccg atgatgcaga cagttgccca actatccctg cttcatatcg ccttgttgga
     9901 aggagatttt gagcaagcgg agcaatatgc tagtattcga caggtcaaat cgtttctgtc
     9961 tctcaaactg tccaatcttc aagtgataca agcctggtat caatttaagg taaggaaaga
    10021 tgtggttcaa actcacaagg ctatgaagat tgctagacag aaaatggata gtagtcgaat
    10081 gttgagggac gagaaacgct attatgaaaa cttgttagca gagctggaaa aggaaatgtt
    10141 agaaggaact gaagatggaa ctagaaattt ctgatttccc aggctgtaag attgctttgt
    10201 tttgtgggga taagcttttg actatcttac gcgatgataa ggcaagcatt ccctgggcca
    10261 atatgtggga actgccaggt gggggaaaga gtgtcaggga aaatctgata tagttttgca
    10321 ttaaaagaat atcctttcaa ggttattctt ttttaatttt gtcccaaaat aattgacttt
    10381 gtcccgaaaa tatagtatta tttgtataag gaggaatgaa tcatgaaaaa atcagatgtt
    10441 cttgatttaa taaagtatca ttatgagggc agagagacag aatttagaaa tcaatccata
    10501 gcaattgctc ggaacttcaa taaacatggt gatacacaaa tagcacaata tattatgggg
    10561 ttaatgtctc aaagtgatag attcatgcca caaatagaaa atcctagtga gtatttgact
    10621 cctgctaaac tagatattgg tcctctccca ttgcctcttt caattatgaa tgatttaaaa
    10681 ggaataataa acgctgtcaa tcatcatatt ggaataaata aatttttatt tgtgggatct
    10741 ccaggtactg gaaaaactga aagtgtaaag caggtggcta gacttattgg caaggaatta
    10801 cttgtagtag atttcagtca cttagtagat agtaagctag ggcaaacagt caaaaactta
    10861 gcaactcttt ttaatgaaat caataatctt cctttcaagc aaaattatat catcctattt
    10921 gatgaaattg attccatagt gttggatagg gttaatcaga atgatttaag agaaatggga
    10981 cgagtgactt ctgccttttt aaaggagttg gataggctgt caccagaaat tgtattaatt
    11041 gcaacaacaa atctttttga aaatcttgat aaagcagtta cgagaagatt cgatgccata
    11101 attgactttg accgttatac tgatgaagat aaagtagaag tagctactat tattttaaat
    11161 gagttattaa agcaatttaa gaatgtagct agagatttaa aattatttaa aaaaattatc
    11221 aatagtgcca atgtaatacc gaatcctggt gatttgagaa actcaataag aacgtcattg
    11281 gcatttagtg atccatcaga tccgcatgat taccaaaaac gcctgctaag aagtttgcat
    11341 aatggtagaa atttatctat ttctaaatta tcgaagctag gttttactgt tagagaaatt
    11401 gagattttga caggtgtttc taagagtagt gtatcacgag agttaagcga ggattaaaat
    11461 gaatacatta ttgacattac gaggtaaaag ctttacacaa aaatcaagaa ataatggtat
    11521 gggaccaata actataccca aaaaaacaat aattacattg gaacatttga agtatcttca
    11581 tttttcatta gaagagacga aaacatattg ggagaaaaat aacattatcg atggtatttt
    11641 gatcagtatt tactataatc gaattgttgc caaaagtaac cgtattaatg gctatttcaa
    11701 tgtaggagga gggaatccat tccctaatga tactattgtg ggagcaaaat tcaatgatga
    11761 gaagacaaaa catattgtta ctcattatat ttctagagat gctcttaata aaacaataac
    11821 cgttttaagt aagataattg aagtttttga ggaacatttt gatagagcga taacatgtga
    11881 gatgttttct gattcatcaa cgtttgcttc tataaatttt tcggagtatg gtatttcaaa
    11941 aagcaaattt caacagtatt tgagagattc ttgctttatt gagaattttg gagttgagca
    12001 cactactgtt tctgatattc aaaattctat cgtgacattt tatgatgtcc atacagacat
    12061 ttttagatta ctgaataaat taaatattga tatttcagaa gctaatatca tgaatcagac
    12121 cacggtcttg cttgatgaaa aaaatattga attactacta tcaaaagcgc cctatcttgt
    12181 ctccatgatt gtggaagatt tttctaagtt atctgtagat gatttttctt tagataataa
    12241 tgatttaaaa ataaatcttc catctccaat gaacgaacca gttgttggcg ttattgatac
    12301 cttatttgac aaaagagttt actttaatga gtgggtagaa tatcatgatt ttgtctcacc
    12361 tgatattagc aaagatagcc aagattacaa gcatgggaca gctgtgactt ctttaatcgt
    12421 tgatggagcc aatttgaacc ctaatctgga tgatggttgt ggaaatttta gagtgaggca
    12481 ttttggtgtg tcactacaaa gtggatttaa ttcatttaca atcataaaac agataaaaga
    12541 gattgtttct cagaatgctg atattaaggt ttggaatctt tctttagggt ccaatgatga
    12601 aataagagaa aattttatct ctgcagaggg agcattatta gacgaaattc aatttgagaa
    12661 tgatgtaatt tttatcatag ctgggacaaa tgcttcagta ataaatggga aacgcaaaag
    12721 aataggtgct cctgctgatt cacttaattc tataatagta aattctgttg attttaataa
    12781 tcaatcagtg tcatattcac gagagggtat tgttttatca ttctttgtta aaccagatgt
    12841 gtcatattat ggaggaggaa atggtgactt tataaatgtt tgtgaacctc taggattagg
    12901 tcgtgtagct ggaacaagtt ttgcggcacc ttttattgca aggaaaatgg cctatctcat
    12961 tcatattatg ggacttagcc gagaagaagc gaaggctttg ctaattgatg cagcaatacc
    13021 ctggaatgat aagaaaacat tcactgattt atctttgata ggaaatggta ttgttccaat
    13081 aaaaatggat gatattttat ctacacctga tgatgaaatt aaatttattg tgtcagatat
    13141 ctctagggct tatgatacct ataattatga ttttccagta cctatttcta gtgagagtta
    13201 tccatatgta gctaaagcaa caatgtgtta tttccctaac tgctcaagaa aacaaggggt
    13261 tgattataca aatactgaga tgcagcttac ttttggtagg ttaaagtcag acggtatcaa
    13321 atcgataaat aaagacaacc agcatgccga agattcccca ggatatgtga gagaaaatgc
    13381 tgctagaaat atatttagga aatgggataa tgtcaagcat attggagagt cgtttacttc
    13441 tcgaaagaga gctaaagcaa ttttaaatcc atctaatcct cagtggggga tgagtattaa
    13501 aacaatcgaa cgtttgaaaa gcggtgatgg acaaggagta cgttttggtg tagtagttac
    13561 attaaaagaa ttaaatgggg ttaatcgtat tgaagatttt attcagcaag ctgaattacg
    13621 cggttggcta gttaatagat tgcaagtaga agctcaagta gacctcttta atagtttaaa
    13681 tgaagaaatt gaatttgaat agtggatatt ttatccttat tttagatctt aggaacacaa
    13741 ga
//
LOCUS       DAVMAF010000044        13684 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMAF010000044 DAVMAF010000000
VERSION     DAVMAF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.044"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(779..1648)
                     /locus_tag="VYO97_001383"
     CDS             complement(779..1648)
                     /locus_tag="VYO97_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1754168.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1872..2549)
                     /locus_tag="VYO97_001384"
     CDS             complement(1872..2549)
                     /locus_tag="VYO97_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1754169.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            complement(2507..3613)
                     /locus_tag="VYO97_001385"
     CDS             complement(2507..3613)
                     /locus_tag="VYO97_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1754170.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3724..3978)
                     /locus_tag="VYO97_001386"
     CDS             complement(3724..3978)
                     /locus_tag="VYO97_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754171.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(4318..4764)
                     /locus_tag="VYO97_001387"
     CDS             complement(4318..4764)
                     /locus_tag="VYO97_001387"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754172.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(4837..5162)
                     /locus_tag="VYO97_001388"
                     /pseudo
     CDS             complement(4837..5162)
                     /locus_tag="VYO97_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5396..7114
                     /locus_tag="VYO97_001389"
     CDS             5396..7114
                     /locus_tag="VYO97_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1754173.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            7225..7572
                     /locus_tag="VYO97_001390"
     CDS             7225..7572
                     /locus_tag="VYO97_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1754174.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7850..8686)
                     /locus_tag="VYO97_001391"
     CDS             complement(7850..8686)
                     /locus_tag="VYO97_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1754175.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8699..9328)
                     /locus_tag="VYO97_001392"
     CDS             complement(8699..9328)
                     /locus_tag="VYO97_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1754176.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9338..9979)
                     /locus_tag="VYO97_001393"
     CDS             complement(9338..9979)
                     /locus_tag="VYO97_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1754177.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(10125..11354)
                     /locus_tag="VYO97_001394"
     CDS             complement(10125..11354)
                     /locus_tag="VYO97_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1754178.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11344..12510)
                     /locus_tag="VYO97_001395"
     CDS             complement(11344..12510)
                     /locus_tag="VYO97_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1754179.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12654..13667
                     /locus_tag="VYO97_001396"
     CDS             12654..13667
                     /locus_tag="VYO97_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1754180.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEKGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         4134 a         2932 c         2436 g         4182 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatag cccccctaat
      361 cgcttatttt atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa ggtaaatcaa
      421 ttcctcacaa tgtttgagaa agaagggagg actattccaa cttcaaccca ctataaagca
      481 gacttacaag atagggaacc catctccgat aatatatagt agaatgaaaa ctgcacaatt
      541 tgattggaga attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca
      601 actgcaacct actatagtcc attgaagctg gaatagccca ctgagacttc taaaccactg
      661 ctggagagga gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc
      721 agtctgatcc tctcttactt atagatggtg ctcactcaaa tgttaaagga gctatttctt
      781 agagataaag gaatttgaat acagctactg ccgcaattgc tgctacgata ggtgctacta
      841 ctggtaccca agaataccac cattttgaat cgcctttatg ctcaccaaga actgattttg
      901 gaaggaaagc atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg
      961 taggtcctcc aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca
     1021 cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat
     1081 caactgtttg tcctgcttct attgcctttt gtttcatgaa ttgaagcact tcagcaccaa
     1141 agaagttttt agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat
     1201 taatcaaacc attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg
     1261 aaatagttga gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac
     1321 ggtatgttgc cacaactaag gcttggccaa agatagctcc caagacttgc gcgataatat
     1381 aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag
     1441 ggttgatgtg attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac
     1501 cataaccaac agcgatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa
     1561 cgttggcaac tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg
     1621 catacttcag tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat
     1681 tccttatttt atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa
     1741 aataggattc atagtttccc agttcaacgg acttgtaagc gtttttattc ttcacgaaag
     1801 acctcgttct caaaaccagg aaaaaagccc acatctgtga gcttttcctg gttcctagat
     1861 tctaaaatct attatttttc atcttctttt ttcttgaaga atcctaatcc tagaccagct
     1921 aacaaggtca tgataccagc actagccaag gtagcattag cttctgtacc tgtgtttggc
     1981 aattcctctc tcttacctgt ctcataagtc ggaacttctg ggtctggatt cactggagtt
     2041 tcagtttttg gagtacctgg ttctggagtt ggctctggta ttggagtctc ttgatttggt
     2101 agttctgggt ttggtgtcgg aacacttgga tgctctggat ttggttgtgg gattggtttt
     2161 tctggagtcg gttcacctgg agtacctggt tcctgaggac ttggaatttc tggtttttct
     2221 ggagttggtt tatctggtgt tgataaacgg tcatacctta ccgttatttc tttatcacta
     2281 gagtctgacg taacttcttg tgattcaact gttggaatat ctggatcttt gtacttgtca
     2341 atcttaccag atacaacctc gtcccagttt cctgttgtcc attcaccgta ggttacaact
     2401 cccgtgacct tgttctcaat ttttgtacgg cttaaggtta caggttgaac aacatcttct
     2461 tttacatttt ggttcgtaac tttatcaacg taatgaatga tacgcgttat agtcttcgtc
     2521 tcagtagagg ttgctgtttt gggaaccact gtttcctcaa cattctcacg gtagtaatag
     2581 tcaactgttg caccgtcttc tggtacgcat ttgcaggagt tgctaccaag gtgtatgttg
     2641 ttttccttgt gataactcgg tcttctttgt cctcagttgt tgttttccct tcaatagttt
     2701 ttgattctgt ggtatactca gaacctatcg ctaaatcagc ttttataaca gactctgcca
     2761 acttctcttg gctaccttct ttatagtaat tggctgttac tgtagcagtg gttggcgctt
     2821 cgctttctct ataaactaag gtcactgttc taccttcgct tacaatattc ccagttaaac
     2881 ttgcagaatt tgtatctgct tctttaaaag tataatattt tccgtcagta gtagtcatgc
     2941 tactgagttt tttatctgtg acattgtagc tggtaccaat cagttgtttt ttattggtaa
     3001 tgtaggttcc gtcactttct ttttctccaa ttccagtatc atttttcaat gatagcaaac
     3061 gcccttgttc atcaacatag cgaactttca cattttctga gattagttct gccaattctg
     3121 aggttttttt ctttttcttg atttcttcgg ttattttccc tttctcttct tcgggaatat
     3181 ttagttttgg aatgattttt tcaacaacgg ttcgtgatgg ttccacagta tcttggatga
     3241 ctgaaaagtc agctagaatt gggagattat actgaacacg gtgactttga gtgtttactc
     3301 ctactctttc attattctct gaaaatactt gtacggtata agaaacaaca tcttttccta
     3361 atagaacatc cccagtagag aaatagccgc cttttcctag tttgctatct ccagagccca
     3421 cttctttcct aatcttatca gatagttttt taccagtcag tacattcgtt cgcacaatcc
     3481 ctttgtctac ccctacaaag tgggagaact ttttgaactc ttcagaacca gatctagccc
     3541 aaccattatt aagggcattt gcttttgtat ttgtattctc tctcaaaagt ttggcgatta
     3601 gaattatatt catcggcact tagagttgct gctatatctg actcttgaat accaacttcc
     3661 ttactaccat ttctagcggc agtatatgtg tatttatctg tttctacttc tagctgatta
     3721 tccttaacat gataggtaat tttctcctgg tataagagtc ttcagtagtt tatatactct
     3781 gtcatcatct ttagctttag aagcagctga gtttagccaa gttggggtat taggagtatc
     3841 tttatcagcc tgtttcaaga cattattcaa gtcatttgct agcctattgg ttatcctcac
     3901 tgtgaaagtt ttcttccagt ctgaaccact tttattagac ttagatatgc tcatgaaatc
     3961 ctggtcaaca ttcaacacaa tatcgccttg ttttttagca tcttccgttg cttctgcttc
     4021 aaattgatca tggagttttt gttgctctgg tgtatagttt ttttatttac acgatttaat
     4081 gtactatttt ttattttagt attaagaact ccattaatac gatttttaaa ctctgcttca
     4141 aaaaatccct taatttgttg gtgcgcaagt tcaacattca aacctttctt atacaagtct
     4201 tcattctgaa cgatttcggc tacacggtga atggtttgat tgtataccgt cttctcttaa
     4261 ccagcagtat ccttagtttt aatgactact cgataggttc ctttagaacc gttatagtca
     4321 tctagtttta aaaaagcatt ccaaacattc aaaaatagtt ggaacttccc ttaataaatc
     4381 cgtcactttc tttttcaaga tagcccaagc ttgctcaata ggattcaaat ccggtgaata
     4441 aggtggtaga ggtaagaaaa agtgtttatc ttggatgcag agttcatcca aaatgttctt
     4501 gggatgaaaa ccagcattgc ccatgacaat aacatgaggt gtcttcaaag caggtaggag
     4561 ttgcgttttg aaccactcca caaagaaatc gcttgtcatg cttttcttgt aaatcatggg
     4621 agctataaac tctccgtcta cttgtcctgc aacaattgaa gttcgctcaa aacgacgtcc
     4681 gctaatcttt tcatagactt tctcccctct aggagcccct gcataaggac gatagagata
     4741 gcggtcgatt cctgtttcat caatataaac aggtgctagg tgctttaaac tattaaaatt
     4801 cttaagaaat aaggctactt ttctgggtct tgttccttaa agctcctcat ctttttttaa
     4861 agtgacctta atctgcttta aagctgccca tactgaagga acagcacaat caaaatgtgc
     4921 cgcaatttcc cgtaaaaaag catctggatg agcctctaca aaggctttca attcctctaa
     4981 aggaatcttt cggtttttga cgactcgctt tttcctctct atgtgttctt gttcacgtct
     5041 ttcttttccc acgtgaagag agttctgacg ccaacaccaa aaaacttggc tgcctcgaca
     5101 tggctgtgcc cctctttgat gtaatctaat gctcgctgtt taaaatcagt actatatgcc
     5161 atagctttat tataatatgc ttgtttaaaa ctaagtaact atatcaatgt tttgttatga
     5221 gaagacttac tttttttact gaaattgaat ttttcctagc ctcaacatca tataacccca
     5281 aaattcaatc tgacctggtt tgaagagatt ttcgaagcag ataaggtttt actaaaacta
     5341 aaaaaaatga tataattaaa ctgattaaat gttttaaaaa taaaggagaa tttgaatgtc
     5401 ttaccaagaa aattaccaga aatgggttga ttttgtggag cttcctgact accttcgtca
     5461 agatttggaa aatatggacg aaaaaactaa ggaagatgcc ttctatacaa atcttgaatt
     5521 tggtactgca ggtatgcgtg gcttggttgg tgctggtaca aaccgtatca acatctacgt
     5581 tgttcgccaa gctactgaag gattggctcg tttgattgag tcaaaaggtg gaaacgagaa
     5641 agaacgcggt gtagcaattg cctacgatag ccgtcacttc tcacctgagt ttgcctttga
     5701 atctgcggca gttcttgcta aacacggcat caaatcttac gtatttgaaa gccttcgtcc
     5761 aactccagaa ctatcatttg cagttcgtca tctcaactgt ttcgcaggta tcatggtcac
     5821 agccagccac aaccctgctc catttaacgg ttacaaggtt tacggtgaag acggtggaca
     5881 aatgcctcca cacgatgcgg acgctttgac tacttatatc cgtgcaatcg aaaacccatt
     5941 tgcagttgaa gttgctgatg tggaaactga aaaagcttct ggcttgattg aagttatcgg
     6001 cgaagctgtt gacatagaat accttaaaga ggttaaggac ataaacatca acccagcctt
     6061 gattgaagaa tttggtaaag acatgaagat tgtctacaca ccacttcatg gtactggtga
     6121 aatgttggct cgtcgtgctc ttgcccaagc aggatttgac tctgttcaag ttgttgaagc
     6181 gcaagcaact gctgacccag acttttcaac tgtaacatct ccaaacccag aaagccaagc
     6241 agcctttgcc cttgctgaag aacttggtcg tcaagttggt gcagatgttc ttgtcgcaac
     6301 tgacccagac gctgaccgtg ttggtgttga agttcttcaa aaagatggta gctacctcaa
     6361 cctttcaggt aaccaaatcg gtgctatcat ggctaaatac atcttggaag ctcacaaaaa
     6421 cgctggaact cttcctgaaa atgccgctct ctgcaaatct atcgtttcaa ctgacttggt
     6481 aacgaagatt gctgaaagct acggcgcaac tatgttcaac gtcttgacag gtttcaaatt
     6541 tatcgctgaa aaaatccaag aattcgaaga aaaacacaac cacacttaca tgatgggatt
     6601 tgaagaaagc ttcggttact tgattaaacc atttgtacgt gataaagatg ccatccaagc
     6661 cgttcttgtc gttgctgaac ttgctgccta ctaccgttct cgtggtttga cacttgctga
     6721 cggtatcgaa gaaatctata aagaatatgg ctactacgca gaaaaaacaa tctctgttac
     6781 tctttcaggt gtcgatggtg ctgaacaaat caaagcgatt atggctaaat tccgcaacaa
     6841 tgctccaaca gaatggaacg caacagctat cactgtcgta gaagacttca aggcacaaac
     6901 tgctactgtt gctgacggta ctgttacaaa cttgacaact cctccaagtg atgtgttgaa
     6961 atacacactt gctgacggtt catggattgc cgttcgccct tcaggtacag aaccaaaaat
     7021 caagttctac attgcagttg taggggaaac caacgaagaa tcacaagcta agattgctaa
     7081 catcgaagca gaaatcaatg catttgtaaa ataaagacct ataaaagatg agaccaacca
     7141 acctcatctt ttttcgtatc aaatctaagc aattttagaa actttatggc atactagact
     7201 tatcaatgaa agcgaggtaa tctcatggaa caatttttgg ataacatcaa agaccttgaa
     7261 gtcactacag ttgtgcgtgc gcaagaagct cttgataaaa aagaaactgc aaccttcttt
     7321 atcggtcgca aaacttgccc ttactgccgt aaatttgcag atacattgtc aggtgtcgta
     7381 gctgaaacca aagctcacat ttacttcatc aatagtgaag aaccaagcca actcaatgat
     7441 ttgcaagcat tccgctcacg ctatggaatc ccaactgtac caggttttgt tcatattaca
     7501 gatggacaaa tcaacgtccg ttgcgactct tcaatgtcag cacaagaaat caaagatttc
     7561 gcaggattgt aaaaaatcag ctaacaagtc ttaaaatcac aaaaacgcat aatgtaaggt
     7621 gcaaaaacct tgatattatg cgttttattg ttcctaaaac agttacatca gcatttcatt
     7681 acagaacatt ttaattttgg aagtcaaatc aatttttaac aatgttttag aaatcacagt
     7741 gggctattcc atattcaatt atccatattc aatcgctatt atcgttttcc tagattacaa
     7801 aaaaactacg ttttgctaat caaaacgcag tttcagccac tattttatct tactgtcctt
     7861 cttttacttc tttggttgct acatcttctc caaaccattt ttggctgatt tcttggaact
     7921 tgccgtcctt gtaaagacta gaaaaagctt catttatctt cttaaccaag gttgtatctt
     7981 ccttacgggc tccaaccgca aaagcttctg tttctagtcc aactgtaaag acattataat
     8041 cgtttaaaac accttctgct tctaaataat agtttgcata gacacggtca atcaatagac
     8101 catcaattcg atcgtttttc aaatcaatca aggcttcatt aaaggtttgg tattgattcg
     8161 cttccttatt agcgacaata ttcttcaaaa tttctggatt tgcttcaaag tccgcataac
     8221 cagatgaacc agcttgagct cctaatgtct ttccagtcat atcctttgca gtcgtgatac
     8281 cagatgattt cttcgtaacc aatacctgct cattcttcat atatgagtta ctgaaagcca
     8341 ccttttcacg gcgttcgtct gtagcggaat agccattcca aatcagatca atcgttcctt
     8401 ttgtcaattc agcttctttc aaatcccaat caatcggttg ccaatttacc gtgattccgt
     8461 atttttcaaa aacagctgta gctaaatcaa tatcaaatcc tgcataagaa ccatctttct
     8521 gagcaaatcc cattggaaca aaagtactat caaatccaat agtaatagac ttgttagact
     8581 ggtactttga ccaattatct ccactagttt cgctagaatt tttcccacaa gctactaaga
     8641 acaaagcagt catcagactg actaatacaa gcatccattt tttcatccat ttttcctcct
     8701 atttaggttc tactttcaat aatacatctg cgatattttc agcaaactgc aaatcatggg
     8761 taaccacaat ctgggtcatc ccaagttccc tattttgcaa gattagcttc tccacttcca
     8821 aacgtaattc tggatccagg gcagaagttg gttcatcgta gccaatgatt tctgggtcaa
     8881 tcatcatagc acgcgccaaa gccacccgct gcttttgccc accagatagt gagaaaggat
     8941 aggcctctgc gtgtcctcct agtcctaact gttccaagag ttcactcgcc ttcttctcag
     9001 cctcttcctg cttcattccc atggtcttca caggcgataa agtcaaattt tccagaactg
     9061 atagatgagg aaatagttga aaatcttgga agacaaatcc cagtaggttg cgcttctgca
     9121 attcatccag ctctaaaggt tgtccattat aaaagatttg ccctgaatca atggtttcaa
     9181 gacctgcaag catacgtaaa agagttgtct tacctccacc agaaggtcca acgatagcca
     9241 ggatttgctt ttcaggaata cttagactga aatcagacag gatttgtttg tctccaaaga
     9301 ctttattgat atttcgtaat tctaacatag ctgcccccta tctataataa ctgtactttt
     9361 tctcaacttt tttggaaata attgtcacaa tcccaattaa aatcaagtaa atggctcctg
     9421 ccaagaacat aggaaccaga ctagcatcgc ggttagcagc tgttcgacta gccaagataa
     9481 ggtctgaaat tccgagagca tagaccaaag aagtatcctt gaccaaactc ataacttcat
     9541 taaagacact aggaagaacg atcttggtca cttggggcaa gataatatag cgcactctgt
     9601 caaaagggct aaacttcaag accttggcag cctcatactg tcctcttgga atagtgtcaa
     9661 ttcccccacg gaaaatttct gcaaagtaag ctgcatagtt gagaacaaag gcaataatag
     9721 ctgcaggaag gcggtctaaa cgaatcccaa tacttgggag cacataatag ataaaaatca
     9781 gttgcaagag taagggggtt cctcgcataa cccagatgta aatgttaatc agataatgga
     9841 ggggcttcca atggacttgc aaggcaaagg caatcaaaac gcccaaggga atcgaaaata
     9901 gcaagaccag tgcaaagacc tgtacagtcg tgctcgcacc attcagtaaa ctcggtaata
     9961 tctcaaataa ataagacata ctgcacctcc taaaagtaat tgttcctatt atagcatgaa
    10021 taaacaagat taccaagtat ttttgtaaaa agattctacc atatacaaat aaaaaccgta
    10081 gctatcaaaa gagataaact acagttctat atgtttaaac attcttacaa tctctcaaac
    10141 agttcttgca tttgcacatc atctggaacc agttttaagt aagtgtgagc atggactttt
    10201 gcttcttcaa aatgtcccaa ttcacgcaag agatagatat agtgttccag aaattctgga
    10261 ttgtccttca aatctcctgt caactcttga taatgctcat aagcagtatc caaatcgtcc
    10321 atttcttgat aagaacgagc aatcatccac ttggtcaaaa gattttctgg ctcctcactc
    10381 tgcaagtcta gaatatcctc ataacgctcc tgctccagat aaatagtggc taaacgaagc
    10441 aagatttctt ctgtatcctc agcgtcttct tttgcagtaa ggagataatt ttctgcacca
    10501 ctagcatcat gcaattcata agaaaattgt gaagcagcta gcaagaggcg agtttcaaag
    10561 ggatttttct ctaatccttg cttagcgata cgcagggctt cttgaacttg atgttcctta
    10621 tgtaaagcct gactgtaccc atactcatag ccttcaaagt caggagaaat ggtatcaagc
    10681 tgcttaaagt agagggtggc tttttgatat tcttcttgat caaaataaag actggccaac
    10741 tcaaaagctg ttaagtcatc gtattctaac tccagggctt tttctaaaaa ctcagtagcc
    10801 gtttcaaatt tccctaactg agcataggca aagccaattc gttgataggt ggaaatgccc
    10861 gtttgctcat aaatcgagcg attatctaac tgggcatagg cttgaatagc cgcttggtaa
    10921 ttttccaact cactatccaa ctctgccaaa cccaatatca agagagaatc ctctgagtag
    10981 gtcaaggcct ccaataattt ctcacgtgcc acatctgtca aaccttccag ctggtaaagg
    11041 tctgccttca aaaccaaaga cgagacatac cagtcactgt cagcttggat ttcctcaaga
    11101 taggtaaagg cttcttctat ttgaccatcc tcgctagcaa ttgcagctag attaagatga
    11161 acctctggaa aatcctctac aattttcagg taaatttcct tggcctgagg atagaaacca
    11221 atcccttcaa gataagttgc caactcatac agaagattac ttgaatcatt ttctaaagct
    11281 ttggcgaaat aatgctcagc cttagttaaa tcttgctcct ccaaagcctg taacatctgt
    11341 tgactattgt tcactcttga cttcctcacc ctctaattca tatagaccac tgactacctt
    11401 ataccattcg aaaatggctg aaatgacaac cttagcagag gcataaaccg gaataccaag
    11461 taaaactccc cagataccaa acatagatcc tgaagttaac aaaacaaaga gaacattaat
    11521 agggtggatg tttaattgac ttcccaaaat caatggagag acaaaacggc cttcaatagt
    11581 ttgttctacg ataaagacaa tcactacttt caaaagcatg actggaccag caatcaaacc
    11641 caatactaga gcaggaagca tggctagaaa gctaccaaga taagggacca gatttaaaat
    11701 accagcagta acccccagcg taaccgcata gcgtagacca ataatcttga agaagatgat
    11761 aaacattact gctacaataa tagccactgt cacttgccct cgaacatagt tggacaactg
    11821 ttgattcaca tctgatagaa cttgtccaac aggttccttc aattttcttg gaatgaattg
    11881 ggtcaaatag ttacgcaagc ctttcccatc acgcaagaga taaaagagca tgaaaggaac
    11941 gataatcaag gcaacaatca cttgagaagc tccgctaata aaggcactca cccagttgac
    12001 tgcctgagat gaaaccttac ttgccaaaac tgtagcctgg ctagaaaaat tggtcaaaac
    12061 ttgctctaat tgaggtctga aatcatctgg caggtgctgg gctaccaatc cattaacaat
    12121 cctatctata tcttctaagt aaacaggaac gtttcttgca aaggtcaaaa cctgacgttg
    12181 cagatttgga atggcgactg ccaagcccca aatgataaag agagcgatga taacaaagac
    12241 aatagtgata gctataacac gattaacctt atgcttctcc atccaatcaa caataggatt
    12301 caacaaataa tataacaaac cagacaaaat gactggcaac atcacaactg ctaaaaagtc
    12361 taaaacaggt gaaaatagaa aactaatctt acttaaaata aaaagattca gtcccaataa
    12421 taaggttact aaaaataccg taattgcctt gttatctaaa aaccacttga aaaaccaaga
    12481 taggctaaaa tgtttctctt tttgctccat aaatacttcc tttctattgt tctctcatta
    12541 taccattttt gaaacaaaat aactcttttt taaagtgctt acatctaatt ggatatcttt
    12601 aatttacttg tttttgtgat ataatagtct tatctttaca cagaggtata gtaatgaaag
    12661 aaactgttta ttttggaact tatacacgtc gtacttctca agggatttac aaggcagact
    12721 ttgatacaga aactggtcag ctttcaaatc tagaactttt tgcagctgag ccaagtccaa
    12781 cctaccttgc ctttgaccag caccaacatt tatacactgt tggtagccaa gacgataagg
    12841 ggggaattgc agcctatcaa actgacggga ctgtgttaaa tcatgttgtt gaaaaaggag
    12901 ctccccactg ttatgttgct gtcgatgaaa agcgtgattt ggtttacgca gccaactatc
    12961 acaagggaca agtccttgtt tataaacgcc aggaagatgg tagtcttcta cttagtgata
    13021 tggatcaaca cagtggccaa ggtccacatg aaaatcaagc ttccccccat gttcactata
    13081 cagatttaac acctgaccac tatctagtga cctgcgactt gggaactgac caagtcatca
    13141 cctatgacct cgatcaagaa ggaaaattat ctaagctcta tacctatcac agcaagccag
    13201 gagcaggctc acgccatatc attttccata accactataa aatcgcttat ctcatttgtg
    13261 aactcaatag tactatcgaa gttttaatct acgatggcgt tggcgaattt gaacgtatgc
    13321 aggtcatttc aactttaccg gaagcttacg aaggctttaa tggaaccgct gctattcatc
    13381 tctctaaaga cggtaaatac ctctacgctt ctaaccgtgg ccatgattct atcgcagtat
    13441 ataccatcct tgcggacggt agcttagagt tattagaaat cgttccaacg catggtcaga
    13501 ctccacgtga ttttgatttg acacccgacc aaaaatttct cattgttgtc catcaagact
    13561 ctgacaatgc aactgtcttt aaacgtaatt gtgacaatgg tcgtctagca gaactctcca
    13621 acgacttcca tgttcccgaa gcagtctgca tccgttttgc tccttaaaac accataaaaa
    13681 atgg
//
LOCUS       DAVMAF010000045        13564 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMAF010000045 DAVMAF010000000
VERSION     DAVMAF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.045"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..124
                     /locus_tag="VYO97_001397"
     CDS             <1..124
                     /locus_tag="VYO97_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1754181.1"
                     /translation="EGHVDFINTYDLETMAQQVIPKAAFGYIASGAGDTFTSFQ"
     gene            252..1742
                     /gene="lysS"
                     /locus_tag="VYO97_001398"
     CDS             252..1742
                     /gene="lysS"
                     /locus_tag="VYO97_001398"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1754182.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINKPFKRVHMVDAIKEITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            2367..3503
                     /gene="lctO"
                     /locus_tag="VYO97_001399"
     CDS             2367..3503
                     /gene="lctO"
                     /locus_tag="VYO97_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1754183.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      3755..3843
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4058..4726
                     /locus_tag="VYO97_001400"
     CDS             4058..4726
                     /locus_tag="VYO97_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1754184.1"
                     /translation="METQNYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(4876..4962)
                     /locus_tag="VYO97_001401"
                     /pseudo
     CDS             complement(4876..4962)
                     /locus_tag="VYO97_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5053..5835
                     /gene="thiM"
                     /locus_tag="VYO97_001402"
     CDS             5053..5835
                     /gene="thiM"
                     /locus_tag="VYO97_001402"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1754185.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            5837..6466
                     /locus_tag="VYO97_001403"
     CDS             5837..6466
                     /locus_tag="VYO97_001403"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1754186.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVVIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      6675..6763
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7025..7585
                     /locus_tag="VYO97_001404"
     CDS             7025..7585
                     /locus_tag="VYO97_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1754187.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            7586..8971
                     /locus_tag="VYO97_001405"
     CDS             7586..8971
                     /locus_tag="VYO97_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754188.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQAI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            8973..9623
                     /locus_tag="VYO97_001406"
     CDS             8973..9623
                     /locus_tag="VYO97_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1754189.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLLVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            9634..10326
                     /gene="tenA"
                     /locus_tag="VYO97_001407"
     CDS             9634..10326
                     /gene="tenA"
                     /locus_tag="VYO97_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1754190.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            10331..10855
                     /gene="thiW"
                     /locus_tag="VYO97_001408"
     CDS             10331..10855
                     /gene="thiW"
                     /locus_tag="VYO97_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1754191.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            10855..11658
                     /locus_tag="VYO97_001409"
     CDS             10855..11658
                     /locus_tag="VYO97_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1754192.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSKGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            11651..12283
                     /locus_tag="VYO97_001410"
     CDS             11651..12283
                     /locus_tag="VYO97_001410"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1754193.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(12492..13283)
                     /gene="thiD"
                     /locus_tag="VYO97_001411"
     CDS             complement(12492..13283)
                     /gene="thiD"
                     /locus_tag="VYO97_001411"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1754194.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIHPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(13367..13465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         4017 a         2493 c         2924 g         4130 t
ORIGIN      
        1 agaaggtcat gtagatttca tcaataccta tgatttggag acaatggcgc aacaagttat
       61 tcctaaagca gcatttggct atatcgctag tggggcggga gatactttca cttctttcca
      121 gtgattttag cgtcaggttc tttttagttt ttaaagatta tccgtgaatt tcttgcttat
      181 ttatgataaa atgggagtat cgcaaaaaat gactcatcgt attcaatttt gagtaaaact
      241 aggaggatcc catgtctaca gaacatatgg aagaactaaa tgaccagcag atcgttcgcc
      301 gtgaaaaaat ggctgcgctc cgcgaacaag gaatcgatcc tttcggaaaa cgttttgaac
      361 gtactgcaaa ttcacaagaa ttaaaagata aatatgccaa cctcgataaa gaacaattac
      421 acgataaaaa cgaaacagct actatcgcag gacgcttgat aaccaaacgt ggtaaaggaa
      481 aagttggttt tgcccacctt caagaccgcg aaggccagat tcagatctac gttcgtaagg
      541 atgctgtcgg tgaagaaaac tacgaaatct tcaaaaaagc agatcttggt gacttccttg
      601 gtgtcgaagg tgaagtgatg cgtacggata tgggagaact ctctatcaag gcaacccaca
      661 tcacacactt gtctaaggct cttcgtcctc ttcctgagaa attccatggt ttgacagacg
      721 ttgaaacaat ttaccgtaaa cgttaccttg acttgatttc taatcgtgaa agctttgaac
      781 gctttgtcac tcgttcaaaa atcatctctg aaatccgtcg ttaccttgac caaaaaggat
      841 tccttgaagt ggaaacacct gttcttcata atgaagccgg tggtgctgct gcccgtccat
      901 ttatcaccca ccacaatgcc caaaacattg acatggtgct tcgtatcgca actgagcttc
      961 acttgaaacg ccttattgtg ggtggtatgg aacgtgtcta tgaaattggc cgtatcttcc
     1021 gtaacgaagg aatggacgct actcataacc ctgagtttac ttctatcgaa gtttaccaag
     1081 cttatgcaga tttccaagac atcatggact tgacggaagg cattatccaa cacgctgcta
     1141 aatcagttaa gggcgatggc ccagtcaact accaaggtac tgaaatcaag attaacaaac
     1201 catttaagcg tgttcatatg gtggatgcta tcaaagaaat tactggtgtc gatttctggc
     1261 aagacatgac tttggaagaa gctaaagcta tcgctgctga gaagaaagtt ccagttgaga
     1321 aacactacac tgaggttggt cacatcatca atgccttctt tgaagagttt gttgaagaaa
     1381 ctttaatcca accaaccttt gtctatggac atccagtagc tgtatctcca ctcgctaaga
     1441 aaaatcctga agaccaacgc tttactgacc gtttcgagct ctttatcatg actaaggagt
     1501 acggtaatgc ctttactgag ttgaacgacc caatcgacca acttagccgt tttgaagccc
     1561 aagctaaagc caaagaactt ggtgatgatg aagcgacagg aatcgactat gactacattg
     1621 aagctcttga atacggtatg ccaccaacag gtggtttggg aatcggtatc gaccgtctct
     1681 gcatgctcct cactgataca acaactatcc gtgatgtatt gctcttccca acaatgaaat
     1741 aaattcttat cctctggctc ttaccagagg attttttgat tcaaaaagag actgaattta
     1801 aggagaaaat gaagtgtagt atattgaagt tgaaatagta cactttgatt tctaagacat
     1861 tgttagaaat tggtttaaat tccctaatca atttgtgcat gttttatttc attttacgat
     1921 agtacgctga aactgttcaa aaagtactag aaattgactt ggattcccca attgatttgt
     1981 tcagattcac tataaataaa aaattaataa gtgggatagg aagttagcgt caactaggat
     2041 agtatcttgc ttaaacagta tatatgggat tgatataagt ccataggtcc tattagagga
     2101 tgttctggtg tcttattcac ttgctttttt atagtattag tagatagaat cagcaaataa
     2161 aaaagtagtt caaatgcctg gcagcaaaaa atctcaacga ttcacttaat atagactaaa
     2221 gtttagagtt taaggaaagt agggctagta tattgtatga aaaaataaga tggtttttaa
     2281 gcaaaactct tgaaaatgat tgaaaaaaga gttattatag ttgtgtagta aaaagcaaac
     2341 gcttacttaa tcaaggaggg cattttatgt catacaaaac aagcaatgca gaaggtcatg
     2401 tagacttcat caatacctat gatttggagc caatggcgca acaagttatt cctaaagcag
     2461 catttggcta tatcgctagt ggggcggaag atactttcac tttaagagag aatatccgcg
     2521 cctttaacca caaactcatc gttcctcata cactttgcga tgttgaaaat ccaagtacag
     2581 agattgaatt tgcaggtgaa aaactatctt caccaatcat tatggcgcct gttgcggctc
     2641 ataaattggc aaatgaacag ggggaagtgg cgactgcgcg tggtgtgcat gagtttggtt
     2701 ctctttatac aaccagctct tactctactg ttgaccttcc agagatttct gaagcccttc
     2761 aagggacacc ccattggttc caattttact ttagtaagga tgacggtatc aaccgccaca
     2821 tcatggaccg tgtgaaggct gaaggttata aagcgattgt cttgacggca gatgctactg
     2881 taggggggaa tcgtgaagtg gataagcgta atggttttgt cttcccagtt ggcatgccga
     2941 ttgttgaaga atacctgcca gaaggtgctg gtaaatcaat ggactttgtt tacaaatcag
     3001 ctaaacaacg cttgtctcca cgcgatgtag aatttatcgc tgaatactct ggtcttcctg
     3061 tgtatgtcaa gggaccacaa tgccgtgagg acgttgaacg ttcgcttgct gcaggagctt
     3121 ctggtatctg ggtaaccaac cacggtggtc gtcaaatcga cggtggacca gctgcctttg
     3181 actcgcttca agaagtggca gaagcagttg atagacgtgt gccgattgtc tttgactctg
     3241 gtgttcgtcg tggtcaacac gtctttaaag ccttggcttc aggagcagac ttggtagcta
     3301 ttggtcgccc tgttatttat ggcttggctc tcggtggtag tgtcggtgtg cgtcaagtct
     3361 ttgaacactt gaatgcggaa ttgaagacag ttatgcaatt gtctggagct cagaccattg
     3421 aagatgtcaa acactttaag ctccgtcaca atccatacaa cccaaccttc ccagttgacc
     3481 ctcgtgactt aaaattgtat tgataaaaca gattgcctcc actgaatgtg gaggtttttc
     3541 ttcgtgtatt ggaagaataa attgctatcg aaaactatag taaaatgaaa taagaatagg
     3601 acaaatcaat caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact
     3661 attctagttt caatctacta tataaagata tttttcggtc aaatatcttg ctagggcttt
     3721 cattttttgc tatactggtt cagtaattaa ataatagaga catttggggt gctttaagct
     3781 gagatgatac ccattgaacc tgatacagtt aagactggcg aagggaaatg tgaaacgttt
     3841 tttcgtatgg cgaaatgaac gatctaatct tgtaagtcaa ctctaattct tgattgtaat
     3901 tggagttttt tgtttatata aactggatag gcttgtttag ttagctctct gcaaatgctc
     3961 tcgatgtgct ttaaaaggat caagtggaat tacgattttg cttgatattt cttggtgttt
     4021 tattaattac taacaagtaa agagaggaga agtaaatatg gaaacacaaa actatgcatt
     4081 tcagccaggt ttgactgttg gagaattatt aaaaagcagt cagaaggatt ggcaggctgc
     4141 aatcaatcat cgttttgtta aggaactttt tgcggggaca attgagaata aggtcttaaa
     4201 ggactatctg attcaagatt atcacttctt tgatgccttc ttatccatgc tgggtgcctg
     4261 cgtagctcac gcagataaac ttgaatccaa gcttcgtttt gccaagcaac taggctttct
     4321 tgaagcagat gaagatggtt atttccaaaa ggcttttaaa gagttaaaag tagcagagaa
     4381 tgactatcta gaagtgactt tgcaccctgt aacaaaagcc tttcaggatc taatgtattc
     4441 ggcggtggct tcatcagact atgcccatct tttggtcatg ctggtcattg cagaaggtct
     4501 ctatttagac tggggttcta aagatttggc tttacctgaa gtctatattc attcggaatg
     4561 gatcaatctc cacagaggtc ctttctttgc agagtgggtt caatttctgg ttgacgaact
     4621 caatcgtgtc ggtaaaaatc gagaagattt gacagaactt cagcaacgct ggaatcaagc
     4681 agtcgcttta gaattagctt tttttgatat tggttacgat gtctagagaa ggctcatgat
     4741 atttacaaat ttgatgaggt aagactcttc gaaaatctct tcaaaccacg tcagtttcac
     4801 cttgccgtag gtatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
     4861 tgagctgact tcgtcagtct tatctacagg ttcaaagcag tgttttgagc aatctgcggt
     4921 tagcctccta gtttgctctt tgattttcat tgagtataaa tcattcaacg taaatgaaaa
     4981 aaatttctta acgaaagata gagataaatc gaaatcagta gatcgtataa agtgtaaagg
     5041 aaggatttca atatgacaag tttaaaatta ttaaaagaaa aagcaccatt ggtcatttgt
     5101 ataaccaatg atgtagtaaa aaatttcaca gcaaatggat tagtagcact gggcgcctca
     5161 ccagccatga gtgagtttcc agcagattta gaggatttgt taaagtatgc tggtggttta
     5221 ttaataaaca taggaacatt gacagatgaa aattggaaat tataccaagc tgctctgaaa
     5281 attgcagaga aatataatgt cccagcagtt ttagatcctg tagcctgtgg agcaggagaa
     5341 tatagaaaaa aagtagcaga tgatctaatc aacaattata aactagcagc gattagagga
     5401 aatgctggcg agattgcctc tttagtagga atagatgtgg catctaaagg agtagatagt
     5461 gcgggcgtag ataatattga cgaaattgct ctagcagcaa atgagaagtt caatattcca
     5521 atagtagtaa caggtgaagt ggatgccatt gctgttaatg gagaagtggt aacgattcat
     5581 aatggtagtg ctatgatgcc gaaagtcatt gggacaggat gcttattagg agctgtagta
     5641 gcaagcttta tcggactaga aaaaggtcaa gaattgaaat cattagaaac agcaatgttg
     5701 gtttacaata tcgctggaga aatagcagaa aaacgtccca atggacatct tcctggaaca
     5761 tttaaagttg aatttataaa tgccttatat gagattacag atgaagatgt aaaggaattc
     5821 aaaagagtga agtaagatgt ttcataaaga attactaaaa ctatatttta tttgtggaac
     5881 gactacttgc caaggaaaaa atctatatac agtcgttgag gaagccttaa aaggtggtat
     5941 aaccttattt caatttcgag aaaaaggtga gggtgcttta gaaggcttag aaaaacttga
     6001 gctagctatc caaattaaag aactttgtaa aaaatacaat gtgccgttta tcgttaatga
     6061 tgatatcgat ttggcaatgg aaattgacgc tgatggcgta catgtaggtc aagatgatat
     6121 tggtgttgat gaaattagaa aattgatgcc agataaaata attggtcttt ctataagaaa
     6181 cgaagaagaa tttcaacaat caaaagttga atatgtagat tatgtaggtg ttgggcctgt
     6241 atttgatacc cagtcaaaag atgatgctgg tggtgctata ggttatgaag gtcttgaatt
     6301 gatgagaaaa ctattgccac aaatgccttt agttgtaatt ggtgggatac agacgaaaca
     6361 tattaaagac attataaaga ccaatgttga cggcgtttca atcatttcgg caatatcgta
     6421 tgcaaaaaat atagaaaaaa ctgttcggga aatgagtgaa caatagatat ctatccttgg
     6481 attctaagta agtaggattt tcctttttta aaaagctatg tctagattca gcttgaatgg
     6541 ctaattcatt aaacaggtca tttgtttttt gcaaaagaaa gatctgctgt ctttgtgagt
     6601 gcaaggctgt atttccagtt caatttcttg aactagtata aaatgtgtgt tataataatc
     6661 acataattaa ataataaagg catttggggt gctttaggct gagatgatac ccattgaacc
     6721 tgatacagtt aagactggcg aagggaaatg tgaaatgttt ttttcgtatg tcgaaaagaa
     6781 ctgtctaatc ttgtaagctg aactctaatt cgtatctgta attagagttt tttcttttac
     6841 gaaactggat gaagatgata tagtagattg aaataagatg cgaacaaatc aattagaaaa
     6901 ttcaaattaa tttctaggaa tattttagca ttcctgtgta ctgttctaaa ttcaatctgc
     6961 tatacaaagt atttaggtcg gtcttcttga gtgccccact tttcttttaa aaaaggagtt
     7021 ttttatgttg aaaaaatggc agttaaaaga tgttatcttg cttgctttct tgtctatctt
     7081 ttttggtggg gttttcgttg gttcaggata tgtgtataat attctcagtc tactcttaac
     7141 acctcttggt ttgcaggcct ttgccaatga aatcctcttc ggtctctggt gtatggctgc
     7201 gcccattgct gccatctttg ttccgagagt cggaagtgca acgattggag aagtgctagc
     7261 tgcgcttgct gaagtccttt atggtagcca atttggtcta ggagctcttt tgtctggctt
     7321 agttcaaggt ttgggaagtg aatttggttt tatcgtaact aagaatcgct atgaaagttg
     7381 gctctctcta actgctaata gtattgggat tacgcttgtt agctttgtct atgaatacat
     7441 taagttaggt tactacgcct tttcccttcc gtttgtcctt tccttacttg tggtacgttt
     7501 tatttctgtt tatttcttct gtaccatctt ggttcgtgcc attgtcaaac tctatcatca
     7561 gtttgcaact ggaggaaaag catagatggg tctggaacta cgagcgattc agtccccaat
     7621 cttctctgag ccgtttgatt ttacttttca tgcgcaagcc tttaccttgt tagttgggag
     7681 cagtggttca ggaaaatcca gcctttttca agccattgcc caagtcagtt ctcttcccta
     7741 tagtggtcaa gtcctgatag atgggagcga ggtcagtcag ctttctatca tcgcacgtgt
     7801 ccagaaggtt ggcattctct ttcaaaatcc caatcatcaa tttaccatgg agaacttgtt
     7861 tgaggagctg attttcacct tggagaatat cggctatcac cttcaggaaa ttgattctaa
     7921 aatagcagag gttgtccagc aatgtcgttg cgaggcaatc ttacaccgtc ccattcatca
     7981 cctatcaggt ggggaaaagc aaaaagcagc gctggctgtc ctctttgcca tgaatcctag
     8041 ggtctatctc ttggatgagc ccttcgcttc cattgaccgc aagagcagaa tcgagatatt
     8101 ggagattcta aaagagttgg ctcttgatgg gaagacagtt attttgtgcg accatgattt
     8161 atctgactat aaagcctata tcgaccatat ggttgagcta agagacggaa aactaaggga
     8221 agtgtttcaa atcccttcct atgagatgac acaggttgct tcaaaggaag ttgcttctag
     8281 cccggaacta ttccatatgg atagaacgac ttgtgagttt ggtaatcgcc ccctcttttc
     8341 aattgctgat ttcacattct atcaagggat ttcctgtatc ctgggtgaca atggtgtcgg
     8401 gaaatcaacc ctctttcggt ctattcttca atttcaaaag tataaggggc gcattgcatg
     8461 gaaggggaca gtcctgaaaa agaaaaagag tttgtatcgt gatctgactg gtgttgttca
     8521 ggaagctgag aagcagttta tccgagtcag tctgcgagag gagcttcaat tagatggacc
     8581 tgattctgaa agaaatcagc ggatttttca agctttacga tattttgatt tggagcaggc
     8641 agtcgataag agtccctatc aattaagtgg tggtcagcaa aaaattcttc agctcttgac
     8701 catcttgacc agtaaggctt ccgtgatctt gctagatgaa ccttttgcag gtttggatga
     8761 tagagcctgc cattattttt gcaagtggat tgtggaggag aggaatcaag gaagaagttt
     8821 tctgctcatt agtcatcgtt tagacccttt gatttctgtg gttgattatt ggattgagat
     8881 gactagtcag ggtcttcggc atgtgaaaga agtgaccatt accaaaccac ttacatctca
     8941 gagtagcaat acccaagggg aggtgagata gtatggtcaa agtagcaacc cagacaccga
     9001 ttatcagtct cttcttgctg attttatcct tggaaacatc tttcattcct tcgattgctc
     9061 tgaatctttt ggtagtcgca ttttgtattc tctttatgct ctattaccgt cgatttaaaa
     9121 tgttagcttg gatgatcata cttgccattt taccatcttt tgccaactac tgggcagttc
     9181 agttacacgg agatgcttca caggcagtca tgcttggaac gagggccttt gtgacagttt
     9241 gtatcggcct tgtctttgtt tcctctattt cactaaaaga gcttctcttg tacttggctc
     9301 aaaaggggct atcacgctct tggtcctatg ccttgattgt ggtattcaat tcttttcctc
     9361 tcattcagca agaaatcaag tccctcaaag aagcttgcct attacgtggt caagaactac
     9421 atttttggtc gcccttgatt tacagtaagg ttctgatgac agtctttagg tggcgccatc
     9481 tttacctgag agctctatct gctcacggat atgacgaaca tgcacagttg aagaatagct
     9541 atcggacttt ttatattcct aaaaaaacaa aattaatcta cctgcttttc tttttattgc
     9601 ttcaaaccag tctattttta taaaggagtt attatggaat ttacagatat tgcgatggaa
     9661 ttatccaaga aagcttggca ggcttccttt catcatccct ttattttaca attacaagag
     9721 gggaatttag aacctgccat tttccgctat tacctgattc aggatgccta ctatctgaag
     9781 gccttctcag aaatctatca tcttttggct gataagactt caaaccaaga gatgaaaaga
     9841 ctcttgaaac aaaatgctca gggtttagtg gagggtgagt tatttattcg ccaacaattt
     9901 ttcaaggaaa tggaaatcag cgaccaggaa atggagcaac atccaatcgc tccaacctgt
     9961 tatcattaca tttctcatat ttataggcaa tttgcagaac cgaacttagc catcgctttt
    10021 gcaagcttgc ttccttgtcc ttggttatac catgatatag gcaaatcact taatcttaaa
    10081 ccatcaccaa atcctctcta tcaacaatgg attgaaactt atattacgga tgagttagag
    10141 caacagatca gagaggaggg agcactggtc aatcagctct atcgagaaag tgatgagaca
    10201 gataagcaaa aaatgctaga tgccttccac atcagtgttc atatggaagc caagttttgg
    10261 gagatggctt accaacacca gacatggaag agcgatttac agtctttaga aaaaggagaa
    10321 gaatagaaac atgagaaagc accaactaca agttcacaaa ttgaccattt tatctatgat
    10381 gattgccctt gatgtagtcc ttacacctat ctttcggatt gagggaatgg caccgatgtc
    10441 cagtgtagtc aatattctag cgggaatcat gatgggacct gtttatgcct tggctatggc
    10501 tacagtgaca gcctttatcc gtatgacgac tcaagggatt ccgcctttag ctctcacagg
    10561 agcgactttt ggagcccttc tagcaggtct cttttataag tacggtcgaa aattttactt
    10621 ttctgccttg ggagaaattg tgggaacagg tattattggt tctattgttt cctatcctgt
    10681 tatggtactc tttacaggat cagctgctaa gcttagctgg tttatctaca cgcctcgatt
    10741 tttcggagca accttgattg gtacagcgat ttcctttatt gcctttcgat ttttaatcaa
    10801 gcaggaattc tttaaaaaag tgcagggata tttctttagt gaaaggatag actgatgcag
    10861 gaatttacaa atccttttcc tataggctct agttccctaa ttcactgtat gaccaatgag
    10921 atttcttgtg agatgctggc aaatgggatt ttggctctgg gatgcaaacc tgtcatggca
    10981 gatgattccc gtgaagttct tgattttact aagcaaagtc aggctctctt catcaatttg
    11041 gggcatttgt cagctgagaa ggaaaaagca atccgcatgg cagcttcgta tgcaaaccaa
    11101 tcttctctcc cgatggtagt agatgcggtt ggcgtaacga cttcatccat tcgtaagagc
    11161 ttagttaaag accttttaga ctatagacct acggtcctta aaggaaacat gtcagaaatt
    11221 cgaagtcttg ttggattaaa gcaccacggc gttggggtcg atgcgagtgc taaagatcaa
    11281 gaaacggagg atttgcttca agtcttgaaa gactggtgtc agacctatca tggtatgtct
    11341 ttcttagtca caggtcccaa ggacctcgtc gtttcgaaaa atcaggtcgc tgtactggga
    11401 aatggctgtg cagaattaga ctggataaca gggacaggag atttggttgg agccttaaca
    11461 gccgtttttc tcagcaaagg aaagactggt tttgaagctt cttgcttagc agtctcttat
    11521 ctcaatatcg ctgctgagaa aatagttgtt caaggaatgg gattggaaga atttcgttac
    11581 caagtactca atcagctttc gctcctaaga agagatgaaa attggctaga taccatcaaa
    11641 ggagaggctt atgaatagag aagcacttag actatatctg gtaaccaatc gctatcaaga
    11701 ttccgtggaa agctttcttg caaaagttga gacggcctgc cgttcagggg ttaccatagt
    11761 ccaattgcga gaaaaaaatc tcacaaccaa tcaatattat caactggcaa aacaagtcaa
    11821 ggaaataaca gatgcttatc aggtaccctt gataatcgat gatcggttgg atgtttgtct
    11881 tgcggttgat gctgctggtc tgcatattgg agatgacgaa ctcccagttt cggttgcccg
    11941 acaagtcttg ggtcctgaaa aaatcctcgg tgtcaccgct aaaacggtta aaagagctct
    12001 ggaagcggag aaatcaggtg cagattactt ggggacagga gccatttttc cgacaaccac
    12061 caaggaaaat gcacccatca ccctgatttc aaccttgaaa acaatttgcc aaacggttgc
    12121 cattccaata gttgctattg gcggcttgac ttcggagaat attgatcagc ttatgggcac
    12181 tggcatagct ggtgtagctg ttgtacgtga tttgatgcaa gcagaagata ttgaggcaaa
    12241 aacgcaagcc tttttaacaa agttgcatga cattctttcc taattaatac tcaatgaaaa
    12301 tcaaagagta aactagaaag ctagtcgcag gttgctcaaa gtactgcctt gaggttttag
    12361 ataagactga cgaagtcagc tcaaaacagt gttttgaagt tgcagatgga agctggcgta
    12421 gtttgaagag attttcgaag agtataaaaa ctctctaatt cccttaaagt gggaactaga
    12481 gagttttttt cttaatcttt aaaagttgta tggttgactg gaccagaacc atgaccgagt
    12541 tgaggggcat cttgaatagc ttttgtgata aaggccttgg ccttatcaac tgcctggtaa
    12601 agactcttgc ccttggctag ttcagcagta atcactgcag caaaggtaca tccagtacca
    12661 tgggtgtgac aggtttgaat tcgtgggctt tcccagacaa attgttcatt cttggtaaag
    12721 aggaaatctt tagcaccacc tttgagatgt ccgcctttga taaccacaga ctgaggacca
    12781 aattctttta aaatcaggcg accagcacgc tgcatgtctt cggggtcatg gattgaaaaa
    12841 ccaacaatct cttctgcttc aggaagattt ggcgtaataa tagttgctag aggtagtaag
    12901 tttgttttga gatagtctct agcacttgag tcaatcaagg catctccact tgtagcaacc
    12961 ataacaggat caaggacata gggacaatcc agttttttaa gataggggtg gatgatttcc
    13021 atgatttcag tagtagccaa cattccagtt tttacagcct gaggtggaat atcagaaaag
    13081 acactctcca attgggcttt caacatttga ggagaaacgt gctcgattag ctgaacacct
    13141 ctggtatttt gagcgacaag actggttaca acagccattc catagacatc tctcgcttgg
    13201 aatgacttta aatctgccat aatgccagca ccaccactag ggtcagtccc tgcaatggtc
    13261 aaagcaacgg gtaaataagt catctaaaac ctcccaacca actgaagttt gtattcttaa
    13321 gaacaagcta gtctgtttta gaaaggcaat agaagactgc tagtccccat tatcatttcc
    13381 acttccatca gctagcatta tctagattga gtcagaggga tctaacagtc gttaatctca
    13441 gctttacgca cccctagtgg ttgttttctt ttttctttag tatagcatat ttttattgtt
    13501 tatatagtaa tatttgactg caaatcgttt ttcaaatgat taaaattcta tttttcgaag
    13561 aaaa
//
LOCUS       DAVMAF010000046        13350 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMAF010000046 DAVMAF010000000
VERSION     DAVMAF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.046"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..248
                     /locus_tag="VYO97_001412"
     CDS             <1..248
                     /locus_tag="VYO97_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754195.1"
                     /translation="QGLMKIYPQAQTPRKSSKLKPLTVEDKAYNHALSKERSKVENIF
                     AKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            439..2730
                     /gene="pcrA"
                     /locus_tag="VYO97_001413"
     CDS             439..2730
                     /gene="pcrA"
                     /locus_tag="VYO97_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1754196.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2773..3453
                     /gene="radC"
                     /locus_tag="VYO97_001414"
     CDS             2773..3453
                     /gene="radC"
                     /locus_tag="VYO97_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1754197.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3450..4139)
                     /locus_tag="VYO97_001415"
     CDS             complement(3450..4139)
                     /locus_tag="VYO97_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1754198.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDTGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4157..4798)
                     /locus_tag="VYO97_001416"
     CDS             complement(4157..4798)
                     /locus_tag="VYO97_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1754199.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4881..5096)
                     /locus_tag="VYO97_001417"
     CDS             complement(4881..5096)
                     /locus_tag="VYO97_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1754200.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5104..5451)
                     /locus_tag="VYO97_001418"
     CDS             complement(5104..5451)
                     /locus_tag="VYO97_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1754201.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5456..6571)
                     /locus_tag="VYO97_001419"
     CDS             complement(5456..6571)
                     /locus_tag="VYO97_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1754202.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6581..7540)
                     /locus_tag="VYO97_001420"
     CDS             complement(6581..7540)
                     /locus_tag="VYO97_001420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1754203.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7653..8222)
                     /locus_tag="VYO97_001421"
     CDS             complement(7653..8222)
                     /locus_tag="VYO97_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1754204.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8350..9021
                     /locus_tag="VYO97_001422"
     CDS             8350..9021
                     /locus_tag="VYO97_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1754205.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9005..9823
                     /locus_tag="VYO97_001423"
     CDS             9005..9823
                     /locus_tag="VYO97_001423"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1754206.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRNRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9820..10716
                     /locus_tag="VYO97_001424"
     CDS             9820..10716
                     /locus_tag="VYO97_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1754207.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFNHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10760..11734
                     /gene="pta"
                     /locus_tag="VYO97_001425"
     CDS             10760..11734
                     /gene="pta"
                     /locus_tag="VYO97_001425"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1754208.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11996..13293)
                     /locus_tag="VYO97_001426"
                     /pseudo
     CDS             complement(11996..13293)
                     /locus_tag="VYO97_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3910 a         2515 c         2871 g         4054 t
ORIGIN      
        1 atcaagggct catgaagata tatcctcaag cacaaactcc acgtaaatcc agcaaactca
       61 agccgctaac agttgaagat aaagcttata accatgcgct atccaaggag agaagcaagg
      121 ttgagaatat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc
      181 atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat catgaactag
      241 gattctagtt ttgcaggaag tctattgagg tattgagcta gtttatgaaa aaattgggtg
      301 aaaagtcgag tgttttagaa acccacagtg tagtattcta gtttcaatcc actatatttt
      361 gctactcccc gtaaagtttc tattttccct gatttctgat ataatagaaa tattgacttc
      421 aagagtaagg aagagaagat gaacgcatta ttaaatggaa tgaatgaccg tcaggctgag
      481 gcggtgcaaa cgacagaagg tcccttgcta atcatggcag gggctggttc tggaaagact
      541 cgtgttttaa cccaccgtat cgcttatttg attgatgaaa agctggtcaa tccttggaat
      601 atcttggcca ttacctttac caacaaggct gcgcgtgaga tgaaagagcg tgcttatagc
      661 ctcaatccag ctactcagga ctgtctgatt gcgaccttcc actccatgtg tgtgcgtatt
      721 ttgcgtcgcg atgcggacca tattggctac aatcgtaatt ttacaattgt ggatcctggt
      781 gaacagcgaa cgctcatgaa acgtattctc aaacagttga acttggatcc taaaaaatgg
      841 aatgaacgaa ctattttggg gaccatttcc aatgctaaga atgatttgat tgatgatgtt
      901 gcttatgctg cccaagctgg cgatatgtat acgcaaattg tggcccagtg ttatacagcc
      961 tatcaaaaag aacttcgtca gtctgaatcc gttgactttg atgatttgat tatgctgacc
     1021 ttgcgtctct ttgatcaaaa tcctgatgtt ttgacctact accagcaaaa attccaatac
     1081 atccacgttg atgagtacca agataccaac catgctcagt accaattggt caaactcttg
     1141 gcttcccgtt ttaaaaatat ctgtgtggtt ggggatgcgg accagtctat ctacggttgg
     1201 cgtggtgctg atatgcagaa tatcttggac tttgaaaagg attaccccaa agccaaggtt
     1261 gttttgttgg aggaaaatta ccgctcaacc aaaaccattc tccaagcggc caacgaggtt
     1321 attaaaaata ataaaaatcg ccgtcctaaa aatctctgga ctcaaaacgc tgatggggag
     1381 caaatcgttt actatcgtgc cgatgatgag ctggatgagg ctgtatttgt agccagaacc
     1441 atcgatgaac ttagtcgcag tcaaaacttc cttcataagg attttgcagt tctctatcgg
     1501 actaatgccc agtcccgtac aattgaggaa gccctgctca agtctaacat tccttatacc
     1561 atggttggcg gaaccaaatt ctacagccgt aaggaaattc gcgatattat tgcttatctc
     1621 aaccttattg ctaatttgag tgacaatatt agttttgagc gtattatcaa cgagcctaaa
     1681 cgtggaattg gtctaggtac agttgagaaa atccgtgatt ttgcaaattt gcaaaatatg
     1741 tctatgctgg atgcttctgc taatattatg ttgtctggta tcaagggtaa ggcagcccaa
     1801 tctatctggg attttgccaa tatgatgctt gatttgcggg agcagctaga ccacttaagc
     1861 attacagagt tggttgagtc cgtcctagaa aaaacaggtt atgtcgatat tcttaacgcc
     1921 caagcgactc tagaaagcaa ggcacgggtt gaaaatatcg aagagtttct ttctgttacg
     1981 aagaactttg atgacaccac tgatgtgaca gaagaggaaa ctggtctgga caaactgagt
     2041 cgtttcttaa atgacttggc tttgattgcc gacacagatt caggtagtca ggagacatca
     2101 gaagtgacct tgatgaccct gcatgctgcc aaaggtctcg aatttccagt tgtctttttg
     2161 attgggatgg aagaaaatgt ctttccactt agtcgtgcga ctgaagatcc agatgaatta
     2221 gaagaagagc gccgtctagc ctatgtaggt atcacgcgtg cagagaaaat tctctatctg
     2281 accaatgcca actcacgctt gctttttggt cgtaccaatt ataaccgtcc gactcgtttt
     2341 attaacgaaa tcagttcaga cttgcttgag tatcaaggtc tggctcgtcc tgcaaataca
     2401 agctttaagg catcatatag cagtggtagt atttcctttg gtcaaggtat gagtttggct
     2461 caggctcttc aagaccgtaa acgcggtgct gccccaaaat caatccagtc aagcggtctt
     2521 ccatttggtc aatttacagc tggcgcaaaa tcagcatcta gcgaggcaaa ttggtccatt
     2581 ggtgacattg ctctccacaa gaaatgggga gagggaactg ttctggaagt ttcaggtagc
     2641 ggtgctaggc aggaattgaa aatcaatttc ccagaagtag gtttgaaaaa acttttagcc
     2701 agtgtggctc caattgagaa aaaaatctaa ttttccatcc ttctcacgaa taataaagtg
     2761 aggaggattt ttatgtacag tatttcattc caagaagatt cactattacc aagagaaagg
     2821 ctggccaagg aaggagttga agcgcttagt aaccaagagt tgctagctat tttactcagg
     2881 acaggaacac gtcaagctag cgtttttgaa attgcccaaa aagtcttgaa caatctttca
     2941 agcctaacgg atttgaaaaa aatgaccctg caggaattgc agagtttgtc tggtattggg
     3001 cgtgttaagg ccatagaatt acaagctatg attgaactgg ggcatcgtat tcacaaacac
     3061 gagactcttg aaatggaaag tattctcagc agtcaaaagt tggccaagaa gatgcagcag
     3121 gaattagggg ataaaaaaca agagcacctg gtggcactct atctcaatac tcaaaatcaa
     3181 atcatccatc agcagaccat ttttatcggg tctgtaactc gtagtatcgc tgaaccgcga
     3241 gagattctgc actatgcaat caagcatatg gcgacttctc ttatcttggt ccacaatcat
     3301 ccttcaggag cggtagcgcc tagccaaaat gatgatcatg tcactaaact tgttaaagaa
     3361 gcctgcgaac tgatggggat tgttctcttg gaccatttga ttgtctctca ttctaattac
     3421 tttagttatc gtgaaaagac agatttaatc taaagttcat taacgacata gtcaaagagt
     3481 ttcttatctt tgggacgatt ttcaaaaaga agttctggat gccattggac accgagaaag
     3541 gcgacatcat ccgtactcat gacagcctca atgataccat ctttaggatc atgagccaca
     3601 acttttaaat ttggtgctaa gtccttgatg ctctggtggt ggaaggagtt gatatgagag
     3661 atttctccat agatttcttg gagaacggta tctggttctg ttaccaagcg ttgagttgtg
     3721 tactcaacag aagaatcctg ccaatggtct tcgatatctt ggtacaaagt tccacccatg
     3781 gcaacgttaa agagttgggt accacggcag acagagaaaa tgggcttttt ctgtttaata
     3841 gcttccttga tgagggccag ttcgaagata tctctttgaa ggtgatagtc atcgctatca
     3901 atggttttgg gttcgccata gaattttgga tcgacatttt gcccacctgt caagatgagc
     3961 ttgtcaatca aactgatata gtggcaggcc atttcttgat caccaatcgg taggatgatg
     4021 ggaatccctc cagtatcttt aacgccttca acaaagcctt ttgctgcgta gctcatcatg
     4081 atgtcatcat ctggatgagc tttttcgttt cctgtaatcc caataactgg ttttttcata
     4141 aaatgatttt cgctttctaa tcctcttttc gcatgaagta gaggagggtt tggagttcac
     4201 ttgtcaaatc gacatactga acgaccacgt cttttggtaa atgcagatgg actggtgaaa
     4261 aactgagaat tcctttcaca ccagcatcaa ccaagagatt agcaacctct tgtgacttga
     4321 cgctgggaac agttaggata gcagtcttca catcagcatc cttgatttta tccttgatct
     4381 gagaaatccc gtaaatggga atcccgtcag gagtttgggt accgacttca ggatggtcgt
     4441 ctaggtcaaa ggccatgata atcttcatct tgttacgttc gtggaagcgg tagtggagaa
     4501 gggcatggcc catatttcca ataccaacca gcatgacatt ggtaatagag ttgtcattga
     4561 gcaaatcggc aaaaaatgtc attagttttt tgacatcata gccaaaacca cgacgaccaa
     4621 gttcaccaaa ataggaaaaa tcacgacgta cggtcgctga atcaataccg atagcctctg
     4681 caatttgctt agagttggca cgttcaatct tttctgcatg aaatctctta aaaattcgat
     4741 agtagagaga gagtcttttt gctgtagctt ttggaatagc aaactgttta tctttcacaa
     4801 aatcacaacc tttctattct tctattttat agaaacattg tgaaaaaatc aacaaaaata
     4861 agaaaaaact aagaaaaatc ttagttttga tgtaaaaaat ctgcatgaga tagaaaacgg
     4921 tagaggtctc cgaccagccc ctgataaact tttttgcccc taaaagtcag agaagtcaca
     4981 taaagtgtat ctggtaaggt tacacatcct gacaaagtca acatgagagc ctcatgatcc
     5041 tcatacttga gagtacgctc tacatgatag cagtccttat aggtcagttc aaacattttg
     5101 gctctatctt tccgattttg taaagacacc acgttctacc aagctatcca tgaggaagta
     5161 gaatttttcc tgatgaatat ggtggtcttc tgatttgaaa atatcaacta gacgaaggcc
     5221 aaacttgtca gtgatattga ttttagcccc tgtaagttcc ttgttaatga tgattttgag
     5281 ttggaagcct tcaccgctgt ttggcacttt ttccaaaagg cgagtcagtt catagttacc
     5341 aaccttagtt tcaaaaaagg tgttatcttt gagggtgaat tttttaacag aagggctaag
     5401 agtgtaatcg taacgacaat tttttaactg aatgattttt tcaaatgcca tatggctaac
     5461 ctccgataat ttcttttaag gtttttgcga gggtttgtag gtcttcaacg gtattttgtg
     5521 gcgacaaact gatgcgaagg gattccttca agcgttctga atttgcgcca tacatggctt
     5581 taagaacatg gctggattgg acaacgcctg cagtacaggc tgagccagta gagattgaaa
     5641 ttccagctaa atctagccga aggagtaaga ggtcattttt ctgaccagga aatccaatat
     5701 tgagaacata agggagatga tgttttcctc tattcaagta atactgaatg ccctccagtt
     5761 ctgccagaaa ggcagtttct agattttgta catgttgaaa atgttcttct tgtttttcta
     5821 ggtcttcttt tagggctgca accatgccta cgatggcagg cagattttca gttcctgcac
     5881 gttttttctg ttcctgatct ccgccatgta gataggaatc aaagtccatg ctagatgcgt
     5941 agagaaaacc gattccctta ggaccatgga atttgtgggc agaagcagtg agaaaatcaa
     6001 tgcccaattc ttctgaatga attgggattt taccaatagc ctgaactgca tcaacatgat
     6061 aggcagcagg gtgttgcttg agtatttggc caatttcagc gatgggaagt aggtttcctg
     6121 tctcattatt ggcaaacata gtagaaacca aaatcgtatc gtcacgtaaa gccttttgaa
     6181 tttgctgggc tgtgatttct tgattttctg gctggataat ggttgcttca aacccaaagt
     6241 gttgaaccaa gtaatcaatt gtttcaagga cagcatggtg ctcgatggca gttgtgatga
     6301 tatgttttcc ttgttcttgg tgacgaagac agtagccaat gatggtagta ttattgcctt
     6361 cagtcccacc agaagtgaaa aagatatgtt gaggttttgt ccttagtaac tgggctagtt
     6421 cctgacgggc ttctcgcaag agtttgccag cttgacgacc atgaccatga atactagaag
     6481 gatttccgtg ggtttcttgc ataaccttgg tcatagctga aatagcaact gctgacatag
     6541 gagtcgttgc agcattgtcc aaataaatca aagaatcacc ttatttcttt ttattgtagg
     6601 caaagagtgg gctgactggt tttctttcgt gaatacggac gatagcatca ccaattaact
     6661 cactagcagt gatgtagcat acatttttag gagttttttc ttttgttgct actgaatcag
     6721 tcacaagaat ttctttaata ttagtattgt caagaagctc agcagctccc tcgacgaaga
     6781 gaccgtggct agaaacagca taaatttctg tagctccttc acgttcaacg attttagaag
     6841 cttcagagaa ggtacgtcct gtatttaaaa tatcatcaat caagatagct ttcttacctt
     6901 caacatcacc aataatataa ccttcgttac gagttgcatc gtcttgaggg tagtcgataa
     6961 tggcgatagg agcatcaaga tattcagcca ggctacgcgc acgtttgaca cctgaatttt
     7021 tagggctaac gacaacaaca tctgaaccaa gcaatccttt atcgcagtaa tgttttgcga
     7081 ataggggaac agtgaaaaga ttatccactg gaatatcaaa gaaaccttga acctgaacgg
     7141 catgcaaatc aagagtcagg atacgatcaa ctccagcctt aaccagcata ttggcaacta
     7201 gttttgctgt aagtggctca cgaggacaag caatgcggtc ttgacgtgca tagccaaaat
     7261 atggaaggac aacgttgata ctgtgggcac ttgcacgcac acaagcatcg accatgatta
     7321 acaattccat taggtggttg ttgacaggga aacttgttga ttggatgatg taaacatcat
     7381 aaccacggac actttcttcg atatttactt ggatttctcc gtctgaaaat tgacgtgatg
     7441 atagttttcc aagtgggaca ccaacagctt gggcaatttt ttgtgcaatc tcttggttag
     7501 agttgagtgc gaaaagtttc atgttttttc tatctgacat tatagaccgt cctctgtaaa
     7561 ctttataaat cctagttata tttaccttac atatatgaac tgggatttgt gtatttttat
     7621 cttttctatt ttaccaaaaa atggagatta tttcagctat ttttcatact tttgacaaat
     7681 cgaaccaatt ttgaaggagc tttttggtag gaaatctgat ttttctctaa aaattgtcgg
     7741 aaatcctgtt tgccttgctc atgattttcc acttcaagct ccaattcgta atctgttata
     7801 tcaaagtatc ggctctgatc cagtgccatg agaccaatag ctgttttcat ttcatagcga
     7861 agcgttgtta gacaaccaag aacctgccag ttcttacttt ggataccatg tttcgccaat
     7921 tcatccagta ctagcccttg aggaagttct tccttactca gatagttctc agcatctttt
     7981 agttgcaatt tttggttgta ttccatgttt ccaacactct gcgggacttt gagtgtcaac
     8041 tcagcccagt cttcaaaggt tcgaatgcgc atagcgactt tcttttctcg cagttcaaaa
     8101 tcaggcgtgt cgatgtagta atttgtttga agaacaggag tgacacctgt gaactggtct
     8161 tttagacgat tgtattcatc ttttttcaat agtgttttca attcaatttc taaatgtttc
     8221 atttttctta ccttttttta tcgttgaaag cggatttatg gtataataag cattgtattt
     8281 attgtatatg aatctggaga aaaaatcaaa gatatttttg acggataata tgagaacaag
     8341 ggagaatata tgaccttaga atgggaagaa tttctagatc cttacattca agctgttggt
     8401 gagttaaaga ttaaacttcg tggtattcgt aagcaatatc gtaagcaaaa taagcattct
     8461 ccaattgagt ttgtgaccgg tcgagtcaag ccaattgaga gcatcaaaga aaaaatggct
     8521 cgtcgtggca ttacttatgc gaccttggaa cacgatttgc aggatattgc tggcttacgt
     8581 gtgatggttc agtttgtaga tgacgtcaag gaagtagtgg atattttgca caagcgtcag
     8641 gatatgcgaa tcatacagga gcgagattac attactcata gaaaagcatc aggctatcgt
     8701 tcctatcatg tggtagtaga atatacggtt gataccatca atggagctaa gactattttg
     8761 gcagaaattc aaattcgtac tttggccatg aatttctggg caacgataga acattctctc
     8821 aactacaagt accaagggga tttcccagat gagattaaga agcgactgga aattacagct
     8881 agaatcgccc atcagttgga tgaagaaatg ggtgaaattc gtgatgatat ccaagaagcc
     8941 caggcacttt ttgatccttt gagtagaaaa ttaaatgacg gtgtaggaaa cagtgacgat
     9001 acagatgaag aatacaggta aacgaattga tctgatagcc aatagaaaac cgcagagtca
     9061 aagggttttg tatgaattgc gaaatcgttt gaagagaaat cagtttatac tcaatgatac
     9121 caatccggat attgtcattt ccattggcgg ggatggtatg ctcttgtcgg cctttcataa
     9181 gtacgaaaat cagcttgaca aggtccgctt tatcggtctt catactggac atttgggctt
     9241 ctatacagat tatcgtgatt ttgagttgga caagctagtg actaatttgc agctagatac
     9301 tggggcaagg gtttcttacc ctgttctgaa tgtgaaggtc tttctcgaaa atggtgaagt
     9361 taagattttc agagcactca acgaagccag catccgcagg tctgatcgaa ccatggtggc
     9421 agatattgta ataaatggtg ttccctttga acgttttcgt ggagacgggc taacagtttc
     9481 gacaccgact ggtagtactg cctataacaa gtctcttggc ggtgctgttt tacaccctac
     9541 cattgaagct ttgcaattaa cggagattgc cagccttaat aatcgtgtct atcgaacatt
     9601 gggctcttcc attattgtgc ctaagaagga taagattgaa cttattccaa caagaaacga
     9661 ttatcatact atttcggttg acaatagcgt ttattctttc cgtaatattg agcgtattga
     9721 gtatcaaatc gaccatcata agattcactt tgtcgcgact cctagccata ccagtttctg
     9781 gaaccgtgtt aaggatgcct ttatcggtga ggtggatgaa tgaggtttga atttatcgca
     9841 gatgaacatg tcaaggttaa gaccttttta aaaaagcacg aggtttctaa gggattgctg
     9901 gccaagatta agtttcgagg tggagctatt ctggtcaata atcaaccgca aaatgcaacg
     9961 tatctattgg acgttggaga ctacgttacc atttacattc ccgctgagga aggctttgaa
    10021 accttggagg ctattgagca tccattagat attctctatg aggatgacca ctttctagtc
    10081 ttgaataaac cctatggagt ggcttctatt cctagtgtca atcactctaa taccattgcc
    10141 aattttatca agggttacta tgtcaagcaa aattatgaaa atcagcaggt tcacattgtt
    10201 accagactag atagggacac ttctggcttg atgctctttg ccaagcacgg ttatgcccat
    10261 gcacgattag acaagcagtt gcagaagaaa tctatcgaga aacgctactt tgctttggtt
    10321 aagggagatg gacatttgga gccagaaggg gaaattattg ctccgattgc gcgtgatgaa
    10381 gattccatta ttaccagacg agtggctaaa ggcggaaagt atgcccatac ttcatacaag
    10441 attgtagctt cttatggaaa tattcacttg gtctatattc acctgcacac tggtcgaacc
    10501 catcaaatcc gagtccattt ttctcatatc ggttttcctt tgctgggaga tgatttgtat
    10561 ggtggtagtc tggaagatgg tattcaacgt caggctctgc attgccatta cctatccttt
    10621 aatcatccat ttttagagca agacttgcag ttagaaagtc ccttgccgga tgatttcagt
    10681 aaccttatta cccagttatc aactaatact ctataaaaac tgtctcagag tataattatt
    10741 atcttaaagg agaaaactca tggaagtttt tgaaagtctc aaagccaacc ttgttggtaa
    10801 aaatgctcgt atcgttctcc ctgaagggga agagcctcgt attcttcaag caacaaaacg
    10861 cttagtaaaa gaaacagaag tgattcctgt tttgcttgga aatcctgaaa aaattaaaat
    10921 ttatcttgaa attgaaggaa tcatggatgg ttatgaggtc atcgaccctc aacattatcc
    10981 tcaatttgaa gaaatggttt ctgccttggt ggagcgtcgc aagggcaaaa tgactgaaga
    11041 agatgtacgc aaggttttgg ttgaagatgt caactacttt ggtgtgatgt tggtttactt
    11101 gggcttggtt gatggaatgg tgtcaggagc gattcactca acagcttcaa cagttcgccc
    11161 agctctacaa atcatcaaaa ctcgtccaaa tgtaactcgt acttcaggag ccttcctcat
    11221 ggttcgtggt acggaacgtt acctatttgg agactgtgcc attaatatca atccagatgc
    11281 agaagccttg gctgaaattg ccatcaactc agcaatcaca gctaagatgt ttggcatcga
    11341 acctaaaatt gccatgttga gctattctac taaaggttca gggtttggtg aaagcgttga
    11401 taaggtcgtt gaagcaacta aaattgctca cgacttgcgt cctgaccttg aaatcgatgg
    11461 tgagttgcaa tttgatgcgg cctttgttcc cgaaactgca gctctgaaag ctccgggaag
    11521 tacagtagct ggtcaagcaa atgtcttcat cttcccaggt atcgaggcag gaaatatcgg
    11581 ttacaagatg gctgaacgcc tgggtggctt tgcggctgta ggacctgttt tgcaaggttt
    11641 aaacaagcca gttaatgatc tttctcgtgg atgtaatgca gatgatgttt acaagttgac
    11701 cctcatcaca gcagctcaag cagttcatca atagtgaaaa ctataaagta atatactatg
    11761 ctatactgta gttatgaaac tatgtgcgaa aagcacttcc attaactcct gagaactaaa
    11821 ttactgattg gtgtcaaaaa ggacaacttc taagcgataa tatcctgtct atatacgacc
    11881 tatagaaatc tgtaatatac atgtccgtaa aacgataaat tccctttttg attttaaatg
    11941 agtatgaaaa gagaattttt tggctctttg tcaactgtag tgggttgaag aaaagctaag
    12001 ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcgata aaaatccgtt
    12061 ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg tttgataagt ttgatgagat
    12121 tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg acgattttct
    12181 ctttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc tgctttagat
    12241 tgtcctcaat aagcccgaaa aatttctctg gttccttatt ctggaagtga aaaagcaaga
    12301 gctgatagtg gtgtttcaag tcttccgaat agctcaaaag cttgtttaaa atctctttat
    12361 tggttaagtg catacgaaaa ataggacgat aaaatcgctt atcactcagt ttacggctat
    12421 cctgttgaat gagtttccag tagcgcttga tagctttgta ttcatgggat tttcgatgaa
    12481 actgattcat gatttggaca tgcacacgac tcatagcacg gctaagatgt tgtacaatgt
    12541 gaaagcgatc aagaacgatt ttagcattcg ggagtgaaac agcctgggag actgtttcag
    12601 cctgagccta gaaatttgaa agcgaagctg tttagccaag tcatagtaag ggctaaacat
    12661 atccatagta atgattttga cgcgacatcg gacggctcta tcatatttaa gaaagtgatt
    12721 tcggatgaca gcttgtgttc tgccttcaag aacagtgata atattaagat tatcaaaatc
    12781 ttgcgcaatg aaactcatct ttcccttagt gaaggtatac tcatcccaag acataatctt
    12841 tggaagccga gaaaaatcat gctcaaagtg aaagtcattg agcttgcgaa tgacagttga
    12901 agttgaaatg gccagctgat gagcaatatc ggtcatagaa atcttctcaa tcaacttttg
    12961 cgcaattttt tggttgataa tacgaggaat ttggtgattt ttcttgacga tagaagtttc
    13021 agcgaccata attttagaac aatgatagca cttgaaacga cgctttctaa ggagaattct
    13081 agtaggcata ccagtcgttt caagataagg aattttagaa ggtttttgaa agtcatattt
    13141 cttcaattga tttccgcact aagggcaaga tggggcgtcg tagtccagtt tggcgatgat
    13201 ttccttgtgt gtatctttat tgatgatgtc taaaatctgg atattagggt ctttaatgtc
    13261 tagtaatttt gtgataaaat gtaattgttc catatgaatc tttctaatga gttgtttggt
    13321 cgcttttcat tatagatctt atgggacttt
//
LOCUS       DAVMAF010000047        13339 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMAF010000047 DAVMAF010000000
VERSION     DAVMAF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.047"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            1..1075
                     /locus_tag="VYO97_001427"
                     /pseudo
     CDS             1..1075
                     /locus_tag="VYO97_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1160..2362)
                     /gene="rpsA"
                     /locus_tag="VYO97_001428"
     CDS             complement(1160..2362)
                     /gene="rpsA"
                     /locus_tag="VYO97_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1754209.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2524..2595)
                     /locus_tag="VYO97_001429"
     tRNA            complement(2524..2595)
                     /locus_tag="VYO97_001429"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2561..2563),aa:Gln,seq:ttg)
     gene            complement(2605..2685)
                     /locus_tag="VYO97_001430"
     tRNA            complement(2605..2685)
                     /locus_tag="VYO97_001430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2649..2651),aa:Tyr,seq:gta)
     gene            complement(2735..2965)
                     /locus_tag="VYO97_001431"
     CDS             complement(2735..2965)
                     /locus_tag="VYO97_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1754210.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3133..3777)
                     /gene="pcp"
                     /locus_tag="VYO97_001432"
     CDS             complement(3133..3777)
                     /gene="pcp"
                     /locus_tag="VYO97_001432"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1754211.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3792..4715)
                     /locus_tag="VYO97_001433"
     CDS             complement(3792..4715)
                     /locus_tag="VYO97_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1754212.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4712..5401)
                     /locus_tag="VYO97_001434"
     CDS             complement(4712..5401)
                     /locus_tag="VYO97_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1754213.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5466..6170)
                     /locus_tag="VYO97_001435"
     CDS             complement(5466..6170)
                     /locus_tag="VYO97_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1754214.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(6226..7248)
                     /locus_tag="VYO97_001436"
     CDS             complement(6226..7248)
                     /locus_tag="VYO97_001436"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1754215.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7380..9851)
                     /gene="parC"
                     /locus_tag="VYO97_001437"
     CDS             complement(7380..9851)
                     /gene="parC"
                     /locus_tag="VYO97_001437"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1754216.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDVAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10271..12214)
                     /gene="parE"
                     /locus_tag="VYO97_001438"
     CDS             complement(10271..12214)
                     /gene="parE"
                     /locus_tag="VYO97_001438"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1754217.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12350..12991
                     /gene="plsY"
                     /locus_tag="VYO97_001439"
     CDS             12350..12991
                     /gene="plsY"
                     /locus_tag="VYO97_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1754218.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDYLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13034..>13339)
                     /locus_tag="VYO97_001440"
     CDS             complement(13034..>13339)
                     /locus_tag="VYO97_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1754219.1"
                     /translation="NTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSPDNGMMES
                     FFGILKSEMFYGYEKSFQSLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3870 a         3008 c         2341 g         4120 t
ORIGIN      
        1 aagcgtcgat tcaagtgcta tcactgttca aaaatgatgg tcgctgaaac ttctatcgtc
       61 aagaagaatc accaaatccc tcgtatcatc aaccaaaaga ttgctcaaaa gttaattgaa
      121 aagatttcta tgaccgatat tgcccatcag ctttccatct caacttcaac tgtcattcga
      181 aagctcaatg acttccactt tgagtgtaat tttagaaatc tgcctgagat tatgtcttgg
      241 gacgttgaaa cagtccgggg agtgactgtt tcaatcggaa gatggagatg agctttattg
      301 cgcaagattt tgaaaagctc aatatcatca ctgttcttga aggtagaaca caagctatca
      361 tccgagatca ctttcttaaa tatgatagag ccgttcgttg tcaagtgaaa atcattacta
      421 tggatatgtt tagtccttac tatgacttag ctagacaact tcgctttcaa atttctaggc
      481 tcaggctgaa acagtctccc agactgtttc actcccgaat gctaaaatcg ttcttgatcg
      541 ctttcatatt gtacaacatc ttagccgtgc tatgagtcgt gtgcatgtcc aaatcatgaa
      601 tcagtttcat cgaaagtctc atgaatacaa ggctatcaag cgctactgga aactcattca
      661 acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc gtatgtactt
      721 aaccaataaa gagattttaa acaagctttt gagctattca gaagacttga aacaccacta
      781 tcagctctat aataaggaac cagagaaatt tttcggactt attgaggaca atctaaagca
      841 ggttcatcct ctttttcaga ctgtctttaa aacctttcta aagaacaaag agaaaatcgt
      901 caacgccctt caactaccct attcaaacgc caaattggaa gcgaccaata atctcatcaa
      961 acttatcaaa cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt
     1021 tatcgctctg aacatcaaaa aagaaaggac aaaatttgtc ctttctcaag cttagctttt
     1081 tttcaaccca ctacagttga caaagagccg aaaaatccct tgtttagtaa acaaaggatt
     1141 ttgtgaattt tcaatttgat taaagttcga tatcaccaaa caaatcagcc attgaaaatc
     1201 ctgtttgtgt ttctggaagt tcgaaatcac gcttttcttg acgtcttgga cgacgtggac
     1261 gagcagcacg tttttcttct ttttgtcctt cttcttgggc tggacgttct tcaagagctt
     1321 taatagaaag tgatacgcgt tctgcatctg cgttaacttc aagaacttta acttgaactt
     1381 cttgaccaac tttaagagct tcttttggat tttcaatccg tttgtgtgaa atttgtgata
     1441 cgtgaacaag tccatcgata cctggcaata cttcaacaaa tgcaccgaag tcagtcaaac
     1501 gtttaactgt tccttctact acatcacctt tagccaattt ttgctcaacg ccatcccatg
     1561 gtcctggtac tgttgcttta agtgaaagtg atacacgtcc ttcttcttcg ttaagatcaa
     1621 ggattttcac ttcaatttct tcaccaacag ttacaactga ttttggtgat acgttacgtt
     1681 catgtgacaa ttcagtcaag tgaaccaatc cgtcaacacc accaaggtcg atgaaagcgc
     1741 cgaagcttgt gatacgagca actttaccag tcacaacatc accaacagcc aatttaccga
     1801 atacttcagc gcgagctgct gcagtagctg cttcaacaac ttcacgacgt gaaaggatga
     1861 agcggttttc tttagcgtta acttctttga ttttagtatc aaattcttga cctacaaaac
     1921 gctcagcgtt acgtacgaaa cgagtatcca acattgaagc tgggataaat ccacgaacac
     1981 cttcaaattc tactgaaagt ccacctttaa cggcacgcgt tcctttaaca gtaacaactt
     2041 cttcttcgcg accaacaagt ttgtcccatg ctttgcgagc ttcaaggcgt tttttagata
     2101 caaggtatgt aactgtatca gtatctttac caactacttg acgaagtaca agaacatcca
     2161 atacttctcc tactttaaca aagtcattga tatctgcatc acgatcgttt gtcaattcgc
     2221 gaagagtcaa gacaccttca acaccagttc cagagattgc aacgttagct tgagtcgcat
     2281 caactgtcaa tacttcagca ctaacaacat caccagtctc aacttgacta acgctattta
     2341 gcaaatcttc aaattcgttc atctaaaaaa tcctccaaca atcaagtttt tcttaaactt
     2401 gacatactta taattttttt cctaagcacc cgcaaggaca tgttcatatc attcacgtct
     2461 ttcaattata aaaataaaat accctaagat attttgcttt ctatcttgat tattacctaa
     2521 gaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     2581 cttggctata ccccattaat gaaatggaga gagagggatt cgaacccccg aacccgaagg
     2641 agcggattta cagtccgccg cgtttagcct cttcgctatc tctcctacaa tcaacatgga
     2701 ctattatatc atgaaaattt caaaaaaaca agacttattt tgctaaatta taattttcaa
     2761 tcaaaatttc cacagctttt tccaattttt tataaaaact atcgacattc ccattcttta
     2821 taatggcaaa ttgtccgtct aattcggcaa tttctccgat aactttttta ccgatagtca
     2881 atgtataacc ttcaaaactg tctttaccaa cattaacttt ggcatccgct acttgaattt
     2941 cgattttttt atctttcttg ctcatttcat acctctcttc actttttcta ttttacaaga
     3001 aaatcctaaa actagcaaga aaaaactcta tatcaggctt ctctgatata gagttttatg
     3061 taaacaacct caattgattt agcatagtta atagttagta aacaagcata attgactatc
     3121 ctttttaggt tatcagtgag tctcaccgcc tactaactgc aaatccttat ctttgtagtc
     3181 tacaatagct tcaatagcta cctctatgcc tcttacaata tcacatagac tcattcctgc
     3241 agtattcggc ttatttacta cttgttccat catatatgga atatgcatga aacctgctct
     3301 catattaggg aattttttat ccactaaata aagagcttgg tacatcaaat gattgcaaac
     3361 aaaggttcct gcactattgg atacaactgc cggaagtccc tgttttttga tagcttgtac
     3421 catcgctttg ataggtaaac tactaaaata ggccgatgct ccatcaatac gaatcggtgt
     3481 atcaattggt tgattgcctt cgttatcagg tatgcgagca tcatcttgat taatagccac
     3541 tcgttcaggt gttaagccgg tcctgccgcc tgcttgtcca atacaaagta cagcatctgg
     3601 ttgatatcgt aatatttctg cctctaaaac ttctgacgac ttataaaaaa ccgttggaat
     3661 ttctacccag cgaacttcag ccccattaat ctcagatggt aataatttta cagcctccaa
     3721 agctggatta atcttttcac ctccaaaagg attaaaacct gtaaccaata ttttcatttt
     3781 attttccttt actaaaatgc gagaaagtac attaagaata tgtgaataac aatcattact
     3841 agagcaacac ctgcttgagc ctttataacg ccattctgat ctttcatatc catcaatgct
     3901 gctggtagag cgttaaaatt agcagccatt ggggtcaata aggtcccaca ataacctgct
     3961 gtcatggcaa gagcaccagc cacaattgga ttagctccca gagcaaatac aaagggaact
     4021 ccaacacctg ctgtaataac ggtgaatgct gcaaaagcat ttcccataat cattgtgaat
     4081 agaaccattc caagaacata agccaaaact cctataaagc gactatctga aggaacaata
     4141 ccgctaatca gatgagagat aacatcacca acacctgcta cagtaaaaat agcccccaaa
     4201 gcccctaata attgaggaac aatcccactt gttgaaactt gctgagtcat tcgattattt
     4261 tctgataaca gactcttagg gtgactattg gtaatcacaa gaacagaaat tgtagcaaac
     4321 aaggcggcaa ggctaatcga aatcttgcta aattctggaa tcatttgcgc taagaccaac
     4381 gcaagtattg ccatcagcat aactggaata aaaattttat ttttcaacct gttagattca
     4441 atattggctt tcatttcatc taaggatggc aaggttccga tacggacttg cttaaacaat
     4501 gttaacagcg ataataggat tacaataata ccaatactca tatttggcat ataggaacca
     4561 cctataaacg taatagacaa tagagtccaa aatgcagatg tcccaagtcg aactgggttt
     4621 gttttatctt tataactaca ataggctgta tggagaaatt gacaaccaat cacaatatag
     4681 gtcaactcta atagttgctt tgccaactct gtcatttttg ttctcctccc ctagtctttt
     4741 ttgatatcaa ttttttatca aataaataat tgtaaatccc cactacaata agtgttataa
     4801 cagcaacaat aatagatgta gaagcaatcc ctgcataatt gctttcatag cctaactgat
     4861 ctaatgttcc ccctatcaag aggactcccc cagcacctac aaacgtattt tgagcaaaga
     4921 aatttccaaa attttcattc gcagccgcac gcgcttttat tgtctcatct tcaacctctg
     4981 ttaactttct acctaattga gactctgcag ctgcttctcc cataggttga accaaaggtc
     5041 tgacaaactg agggtgtcct cctagacgaa ttgaaaagaa accagctaac tctcgaataa
     5101 agaaataaac tgtatagaag tttccaactg tcagaccttt aatctttcga atcaaatcga
     5161 ttgatcgttg cttgagtcca aaggtttctg acagccccac aagaggcaag gtaaccataa
     5221 aaatcgtgag cactcgctga ttgctaaatt cttttcccaa aatctccaaa aattcaacga
     5281 gagaaacacc tgaaactaaa gctgtaacca aaccagctaa gactactgtt gcaattgtat
     5341 caaattttaa aataaaaccc acaacaatga ttgctattcc tattaatcta atccactcca
     5401 tatcaaactc ctttatattc aaaatgacag tattttaaaa attttatcaa gatcaatacc
     5461 attccttatt taatgtattt ttctagttct ttttggtatt tgctattgga ttccaatttt
     5521 tctttttgcc attttttaaa aacctcgtta tattcttttg ttgtaacaat atctttttgc
     5581 aatttcattc ctttaaagat atatggttcc cccttaatac caacttgtga gtatggtttt
     5641 gagaatggta ctacgttact tacaactgga gaaccaccag atgaagctgt tggcatcaat
     5701 aatgaactat ctgtcgacca agcttgagct ttggcatatt tttcatatct cttctctagg
     5761 tcagtggtct cagaaacagc atcttctaac aatttcttat atttatccaa accaggttta
     5821 gctacaacat ccttatcttt tcctttcgta atgccaaggt gtttcatggc agaaccagat
     5881 tttggatcca taatattcaa gtaagacgct ggatcttgat agcttggagc ccatcctgta
     5941 ctgttcaaat catagtcttt ttgagaagga gcaacattac cgtatttatc attttccatc
     6001 aaaccatcaa taacatttcc aataacgtct gtcctcgatg ttcgagtcgc tatactgtag
     6061 cccaatgatg ctggatctac tgcatagaca taagaaaatg ttgtcggtgc atctgcttct
     6121 ttatcagttt ttccacaagc cactaaaata gctgacgtgc tcaggaccac tcctgctgtt
     6181 aagagccact ttttctattt cataaaaaat ctcctttggt ttattttaat ctacttttac
     6241 aatccaacct tctggcgctt caatatcgcc aaactgaata cccgtcaatt cattatataa
     6301 tttacgcgtc acaggaccta cttctgtttc actatagaat acatggaaat catcaccatg
     6361 ttgaatacct ccaattggag aaataaccgc tgctgtacca caggcacctg cctctacaaa
     6421 acggtcaaga ttatcaattg gaacatcacc ctcaatagga gttaatccca agcgatgttc
     6481 tgccaaataa agcaaggaat acttggtaat agatggcaag atagatggac tcaatggtgt
     6541 tacaaattca ttatcagctg taattccaaa gaagttagct gatccgactt cttcaatctt
     6601 tgtatgagtt gatgggtcca gatagataac atctgagaaa tgacgtgact tggccatttt
     6661 tcctggtaag agacttgcag catagtttcc accaacctta gccgcacctg taccatttgg
     6721 tgctgcacgg tcgtactcat cctgaatcaa gaagttggtt gggaccaaac cacctttaaa
     6781 gtaatttcca actggcatag caaagatggt gaaaatgtat tcttctgccg gttttacccc
     6841 gataatatct ccgacaccaa tcaaaagagg gcgaagatat aaggttccac ctattccgta
     6901 tggtggtacg tattcttcat tcgcacggac aactgcttta caagcttcta caaacatgtc
     6961 tgtcggaact tgtggcatca agagacggtc acatgtacgt tgcaggcgtt tagcattttc
     7021 atcaggacgg aacagttgaa cactgccatc cttagtacga taagctttca aaccttcaaa
     7081 tgcttgttgt ccatagtgaa gacttggaga agactctgaa atatgcaaag ttgcatcctc
     7141 tgtaagctct ccttgatccc attgtccatt tttgaaatga gcaagatagc gataaggtaa
     7201 tttcatatag gaaaaaccga ggttttccca atcaatcgtt actgtcatat tccactcctt
     7261 attctaaaaa cctatttctt atattctaca ctatttttct aaaatagcaa gtatattttg
     7321 taattttcag aaaatttctc caataaaaac cagctcttag aactgattct tcatttcact
     7381 tatttatctt cagtaactac ttcctgaaga taagcgtcaa aaacttcttc atctgaaatc
     7441 gtgtcagaaa tgaagcttcc attgctagtg cgttctgaca agttcaagtc ttgcaatcgg
     7501 ctttcataga ttgttccttt attggattgg acaagcagag tttggtcgtt cacatccact
     7561 tccgtactga agaaatcgcc aacaaatcct tgctctgcaa ctgctcctgc caagaagaca
     7621 cgatgcggtt tgtttttcaa ctcacgcaag acttgtaatc ctcgtttggc acggctggtt
     7681 gctagaattt cctcaatgga aacacgtttc aagcttccac gctgggtcaa gaggtagaag
     7741 gacgaagtat tacagataaa gccagattgg aggacatcat cttctttcaa attcatagcc
     7801 ttgacacctg ctgccttagc accgacaacc ggaacctctt cgatattgaa acgcagggca
     7861 taaccatttt gactaaccaa gacaacatca tctagtttaa tcggagccac tgctacaatc
     7921 tgatctgtat cgtctttgag cttagcatac ttgacagact tagatctata ggtccgccat
     7981 ggagtaaatt cttttcgctc tacccgtttg atttgaccaa ggcgagtcgc tgcaaagtag
     8041 gttgtcgcat cgtcaaactg atccagtact tccacataaa ggatttcttc attcgtttca
     8101 aagtttgtga tggtttggct cagatgctct ccgatgtcct tccaacgaat atctgccaac
     8161 tcatggattg gtctgtagat gacatttcca agacttgtga acatcaagag gtgctgggtt
     8221 gtcttggcag attgaacaaa aatcaaacgg tcatcatcac gcttgccaat ttcttccaag
     8281 gtggaagccg caaaggaacg tggactggta cgcttgatgt aacctgcctt ggtcacgctg
     8341 acgtaggtat cttcctcagc gataagacta gctgtatcaa tctcaattgc tttcgcagta
     8401 tcttctaaag aactcaaacg aggagttgca aatttcttct tgacctcacg aagttctttc
     8461 ttcatgagat tgtacatagt cctttcatca ccgataatag ccgccagcat agcaatcttc
     8521 tcacgaagct ctgcttcttc ttcctgcaag acaaccacat cggtattggt caaacggtac
     8581 agttgcaaag ttacgatagc ctcagcctgt tcttccgtaa aatcatagct aactttgagg
     8641 ttttccttgg cgtccgcctt attctcagaa gcacggataa gagcaatgac ttcatccaaa
     8701 atcgaaatca cacgaatcaa accttcgacg atatggagac gtttctcagc cttttctttg
     8761 tcaaagcgtg aacgcgccaa aatcacttct cgacggtgag cgatgtagct agacaggatt
     8821 ggaacaatcc caacctgacg aggtgtgaaa ttgtcaatcg ccaccatatt aaagttgtag
     8881 ttgatttgta ggtcggtgta cttaaataag tagttgagaa caagctcagt attagcgtct
     8941 ttcttaagtt cgatagcgat acgaagacca tcacggtcag actcatcacg aacctcagca
     9001 attccagcta ccttgttatt aacacgaaca tcatcgattt tcttgactag attggcctta
     9061 ttgatttcat aaggaatctc agtaataacg atttgttcct taccaccttt tagcttttca
     9121 atttcagtct tggaacgaac aaccacgcgc cctttcccag tctcataagc tttcttgatt
     9181 tcatcacgac cctgaataat agcccctgta gggaagtctg gtccaggcaa gaattccatg
     9241 agtttatcaa tctttgcagt tgggtggtca atcatgtaaa ctgcaacatc tatgacctca
     9301 gctaaattat ggggaggaat gtctgtggca taaccagccg aaatcccagt cgaaccattg
     9361 accaagaggt ttggaaaggc tgctggcaag accgttggtt ctttctccgt atcgtcaaag
     9421 ttccatgcaa aaggaactgt ctttttctcg atatcctgaa gaaggtagcc tgcaatttca
     9481 gacaaacgtg cctcagtata acgcatagcc gcaggaggat ctccgtccat agaaccgtta
     9541 ttaccgtgca tttcaactag aatctcacga tttttccagt tctgtgacat acgaaccatg
     9601 gcatcataga tagaagaatc cccgtgtggg tggaaattcc ccatgatgtt cccgactgac
     9661 ttggccgact tacggtagct cttgtcaaaa gtattgctat ccttattcat agaataaaga
     9721 atacggcgct gaaccggctt caacccatca cgaatatctg gcaaagcccg gtcttgaata
     9781 atgtacttgg agtagcgacc aaagcgctct cccatgatgt cctccaggga catgttttga
     9841 atgttagaca taagatacaa agcccataaa ataccaagtg aaaatagaaa attcttgaag
     9901 taagcaaact cacaagagaa tttatctttt tcacacagta tctagggcgt gttcaactcc
     9961 tttcaaagaa tgtagagtag gtttttatgc agtaaaagat attttacggg aattcgtccc
    10021 gtgttcagtt acgataagta accaaactat cctgtttgta tttttcaata tgaaaatctg
    10081 gttttccaaa attagtctta gtttgtgtct tagccgctcc cttaaacgcc tctttgagat
    10141 aagcactcat agaagattct tcattaataa tcctgcaatt ttttcaaacc aagattttca
    10201 aactgctttt ttacatagtc attcacatcc gactctaatt tccagtttac taacatatta
    10261 ttttctttca ttaaaacact gtcgcttctt ctagcgtaaa cttgacatta tcttcaatcc
    10321 atttacggcg tggttctacc ttatctccca tgagaacatt gacgcggcgt tcggcgcgcg
    10381 ctaaatcttc aattgtgaca cggatgaggg tacgtgtttc tgggttcatg gttgtttccc
    10441 agagctggtc cgcattcatc tcaccaagtc ctttgtatcg ttggagggta gcgcctttac
    10501 cgaactgttt acggagttct tctagttctc cgtccgtcca agcgtaggcc acttcttctt
    10561 tcttgccttt acctttggac atcttgtaaa gaggtgggag ggcaatatag acatgacctg
    10621 cctcgactag cggacgcatg taacggtaga aaaatgtcaa gagcaaggtc tggatatggg
    10681 caccgtcggt atccgcatcg gtcatgataa tgatcttatc atagttggca tcttcaatag
    10741 agaagtctgc tccaacaccc gcaccaatgg tataaatcat ggtattgatc tcttcatttt
    10801 tgaggatatc cgccatcttg gccttggctg tattgacaac cttaccacga agaggtagaa
    10861 tagcctggaa cttgcggtca cgaccttgtt tggcagaacc accggcagag tccccctcaa
    10921 ctagatagag ttcattctta gcaggattct tagattgggc tggggtcaat ttcccagaca
    10981 acaagccctt atctttcttg tttttcttcc catttcggct ctcatcacgc gccttacgtg
    11041 ctgcttcacg agcatcacgg gccttgatag ccttgcggat gaggttagaa gctaattccc
    11101 cattttccat aaggaaaaag gtcaacttat cagccactat tccatccaca actgggcgag
    11161 ctagggggct tcctagttta tccttggtct gtccttcaaa ctgcaagtgt tcttcaggaa
    11221 ctaagataga aagaacggcc gctagtccct cacgatagtc tgaaccttca aggtttttat
    11281 ctttttcctt gagaagacct gttttacgtg catagtcatt catgaccttg gtaatggcag
    11341 acttgagtcc tgtctcgtgc gttccaccgt ccttggtgcg aacgttattg acaaaggata
    11401 gaatgttatc tgagaatccg tcattgtact ggagggctac ttccacttga aaaccattgt
    11461 cttccccttc aaagtaaaga actggcgtca agatttcctt atcttcgttg agataagaaa
    11521 caaaatcttg tactccattc tcatagtgga actcaatcgc ttcatctgtt cgcttgtccg
    11581 ttaaagacaa ggtcacattt ttcaagagaa aggctgattc attaaggcgc tctgaaatgg
    11641 tattgtactt gaaatctgtc gtagaaaaga tagtcgcgtc aggcataaaa gtaactttgg
    11701 tgcctgtttt agacttgggt gctgtaccga ttttcttcaa agtcgtgaca ggttttccac
    11761 cattttcaaa acgttgcttg taaactgcgc catcacgggt aatttcaact tctaaccagc
    11821 tagaaagggc gttaacaacg gaagaaccca ctccgtgaag tccacctgat gtcttatagc
    11881 caccttgacc gaatttccct ccggcatgaa gaatggtaaa gataacttca acagttggaa
    11941 ttcccatagc gtgcatacct gtcggcatcc cacgtccatg gtcttgaacc gttagactac
    12001 cgtctttatt gatagttaca tcaatacgat caccaaaccc agacaaggct tcatcgactg
    12061 cattatcaac gatttcccaa actaggtgat gaagaccagc gccatcggtc gatccaatat
    12121 acatccctgg acgttttcgg accgcatcca acccttctag cacctgaata gcatcatcat
    12181 tataattgtt aatattgatt tccttttttg acacaaggaa cctcctattc gttcatcttt
    12241 actattctac aggttttcca aggattttgc aaaatttttc tttctccgat gtgacaattt
    12301 cagcagagat tctctgcttt tctttcccaa ttcatgatat aataggagta tgattacaat
    12361 agttttatta atcctagcct atctgctggg ttcgattcca tctggtctct ggattggaca
    12421 agtattcttt caaatcaatc tacgcgagca tggttctggt aacactggaa cgaccaacac
    12481 cttccgcatt ttaggtaaga aagctggtat ggcaaccttt gtgattgact ttttcaaagg
    12541 aaccctagca acgctgcttc cgattatttt tcatctacaa ggcgtttctc ctctcatctt
    12601 tggacttttg gctgttatcg gccatacctt ccctatcttt gcaggattta aaggtggtaa
    12661 ggctgtcgca accagtgctg gagtgatttt cggatttgcg cctatcttct gtctctacct
    12721 tgcgattatc ttctttggag ctctctatct tggcagtatg atttcactgt ctagtgtcac
    12781 agcatcgatc gcggctgtta tcggggttct gctctttcca ctttttggtt ttatcctgag
    12841 taactatgac tatctcttca tcgctattat cttagcactt gctagtttga ttatcattcg
    12901 tcataaggac aatatagctc gtatcaaaaa taaaactgaa aatttggtcc cttggggatt
    12961 gaacctaacc catcaagatc ctaaaaaata aaacgccagt tctgtactgc ccccaaacag
    13021 ttagacaatt aatttatccg aaggatttag ttctgtattg cacagggcta agtcctttta
    13081 gttttacctt aattcgttta ttgttgtagt aatcaatata gtctataatg gcttgttcca
    13141 attgcttaag cgactgaaac gacttctcat aaccgtaaaa catttccgat ttcagaatcc
    13201 caaagaagga ctccatcata ccattgtctg ggctgttacc cttacgtgac atggatgctt
    13261 gaactccctt actctctagg aaccgatgat aagaatcgtg tcggtattgc cagccttggt
    13321 cactatggag aatcgtatt
//
LOCUS       DAVMAF010000048        13276 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMAF010000048 DAVMAF010000000
VERSION     DAVMAF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.048"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..306
                     /locus_tag="VYO97_001441"
     CDS             <1..306
                     /locus_tag="VYO97_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1754220.1"
                     /translation="NTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMES
                     FFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG"
     gene            complement(380..910)
                     /locus_tag="VYO97_001442"
     CDS             complement(380..910)
                     /locus_tag="VYO97_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEV1754221.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPNWKMDLFSEEEKRNYFQTTCEIS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LYNIVNIVSDIHKLGI"
     gene            complement(956..1372)
                     /locus_tag="VYO97_001443"
     CDS             complement(956..1372)
                     /locus_tag="VYO97_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754222.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYYLSDKVPAQGWKIHISSQIK
                     DAVNIFKIVYKLSQLNNCSFKVVKNLEELKKINSPREMSPTANKFITLYPKSESEAKS
                     MICNLTNRLSEFKAPKILSDYQCGMHSLVHYRYGAF"
     gene            1921..2598
                     /locus_tag="VYO97_001444"
     CDS             1921..2598
                     /locus_tag="VYO97_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1754223.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2585..3382
                     /locus_tag="VYO97_001445"
     CDS             2585..3382
                     /locus_tag="VYO97_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1754224.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            3399..4502
                     /locus_tag="VYO97_001446"
     CDS             3399..4502
                     /locus_tag="VYO97_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1754225.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            4503..4583
                     /locus_tag="VYO97_001447"
                     /pseudo
     CDS             4503..4583
                     /locus_tag="VYO97_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4848..5867
                     /gene="galE"
                     /locus_tag="VYO97_001448"
     CDS             4848..5867
                     /gene="galE"
                     /locus_tag="VYO97_001448"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1754226.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESSDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5869..6822
                     /locus_tag="VYO97_001449"
     CDS             5869..6822
                     /locus_tag="VYO97_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1754227.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(6884..7090)
                     /locus_tag="VYO97_001450"
     CDS             complement(6884..7090)
                     /locus_tag="VYO97_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1754228.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            7142..7618
                     /locus_tag="VYO97_001451"
     CDS             7142..7618
                     /locus_tag="VYO97_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1754229.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7627..8298
                     /locus_tag="VYO97_001452"
     CDS             7627..8298
                     /locus_tag="VYO97_001452"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1754230.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8429..8767
                     /locus_tag="VYO97_001453"
     CDS             8429..8767
                     /locus_tag="VYO97_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1754231.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            8770..9399
                     /locus_tag="VYO97_001454"
     CDS             8770..9399
                     /locus_tag="VYO97_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1754232.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9639..10790
                     /locus_tag="VYO97_001455"
     CDS             9639..10790
                     /locus_tag="VYO97_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1754233.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            11103..11852
                     /gene="truA"
                     /locus_tag="VYO97_001456"
     CDS             11103..11852
                     /gene="truA"
                     /locus_tag="VYO97_001456"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1754234.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11842..12615
                     /locus_tag="VYO97_001457"
     CDS             11842..12615
                     /locus_tag="VYO97_001457"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1754235.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKRGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            12593..13054
                     /locus_tag="VYO97_001458"
     CDS             12593..13054
                     /locus_tag="VYO97_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1754236.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKRKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         3807 a         2464 c         2924 g         4081 t
ORIGIN      
        1 aatacgattc tccatagtga ccaaggctgg caatatcaac acgattctta tcatcggttc
       61 ctagagagta agggaattca agcatctatg tcacgcaagg gaaacagccc agacaacggc
      121 atgatggaat ctttctttgg cattttgaaa tcggagatgt tttatggtta tgagaagaac
      181 tttagatctt tagaaaacct tgaacaagct attgtggact acattgatta ctacaacaac
      241 aaacgaatta aggtaaaact aaaaggactt agccttgtgc agtacagaac taaatccttc
      301 ggataaatta attgtctaac ttttggggtg cagtacattt ttggtatata taaaatttgt
      361 aggagctata tctacaattt tatattccca gtttatggat gtcacttact atattcacaa
      421 tgttatacag tgttttttct ctaatattta aggagtgttc tgtttctcga ataaattctt
      481 caaagtttaa cccgtcaact tgttcctgaa caagaaaata atcatccacg atataaaatt
      541 catcagttaa attagtagta taacttttat cggctaattt ttttagcatg tgagcttcat
      601 tttttatatc atcaagagct gtccattctc cttcagcatc ataattcaca aaaggtcttg
      661 actgcttgat gattactttt tgcccgtccg attttctaat tgcccgataa acatttcctt
      721 tatttgatct cttaataatt ttttccattt tgtatttatt tattgcagag tctttacttg
      781 aaatttcaca tgtggtttga aaataattcc ttttttcttc ttctgaaaat aaatccattt
      841 tccagttagg gagactaggg aaattttgtc tcttatcttc tacatagttc ttcctttttt
      901 catctagcaa taaatagatg acttttttat ttttttcatc ataagcttgt tttttttaaa
      961 aagccccata tctataatga actagagaat gcattccaca ttgatagtca gatagtattt
     1021 ttggagcctt aaattctgac agtctattcg taagattaca aatcatactc ttagcttcag
     1081 attctgactt aggatataga gttataaatt tgttagcagt agggctcatt tccctagggg
     1141 aattaatttt ttttaattcc tctaaatttt taacgacttt aaagctacaa ttatttagtt
     1201 gggatagttt atacacaatc ttaaaaatat ttacagcgtc ttttatttgg gagcttatgt
     1261 gtattttcca cccttgtgca ggcaccttat ctgataaata atagtaattc caaatatcac
     1321 cctgttggaa atttttatca cattttaaac attctttctc taataaatcc ataacaaact
     1381 cctaattatt aaaataggct agagacacag cactagccta ttccgtaagt aaagctactt
     1441 attttatata aaatataact agcgacctgt aattaaacaa gtaaaagaac aatacttagg
     1501 gagacctgtg acaatgacat cttcctctgg aagtagttct aattcaaaca atgtttgttc
     1561 catgactgta tcctcctgtt atgataaatt ccagaaagca tagagcaaat tcgtggttcg
     1621 tctgactaaa tttcttatct gaacaccacc ttcaaataca aattttagtc aaacttttat
     1681 tttttctaac tttctaatta tattctacca tgtgttttta tatgtcaaga attttctgaa
     1741 taaactgttt attttttatt ttttgacatt tttgcaaatt gtttaaaatc tttcaattgg
     1801 attcacttat tactttagga cattgaaact tctttcgcca gaggtgctta tattactggg
     1861 aagttgaaga ttgaaatgtg gtataataag aggtaataga gttttggaaa gtgagagaag
     1921 atgatttcaa agagattaga attggtagct tcctttgtgt cacagggggc tattttacta
     1981 gatgtgggaa gtgaccatgc ttatctgcct atcgagttgg ttgagagagg ccaaatcaaa
     2041 agcgctattg caggtgaggt ggtggaaggt ccctatcagt ctgcggttaa aaatgttgag
     2101 gctcacggcc taaaggagaa aatccaagtc cgtttagcca atggcttggc agcttttgaa
     2161 gagactgacc aagtgtctgt cattaccatt gctggcatgg gtggtcgttt gattgctagg
     2221 attttagaag aaggtttggg gaagttagct aatgtagagc gtttgatcct ccagcccaat
     2281 aatcgtgaag acgacttgcg tatctggcta caggatcatg gattccagat tgtagcagaa
     2341 agcatcttag aagaagctgg aaagttttat gagattttgg tggtggaagc aggacaaatg
     2401 aagctatcag ccagtgatgt tcgctttggt cccttcttgt ccaaagaagt cagtccagta
     2461 tttgtccaaa aatggcaaaa agaagctgag aagctagagt tcgccctcgg acaaatccca
     2521 gaaaaaaatc tggaagaacg tcaagttcta gtagataaga ttcaagctat caaggaggtg
     2581 ctccatgtta gcaagtgaag tgatccaagc ttatgaagcc ttttgccctc aggaattttc
     2641 tatggaggga gacagccgtg gtctgcaaat tggcacttta gacaagggta tccaaagggt
     2701 catggttgcc ctcgatattc gtgaagagac ggtggcagag gccattgaaa agggtgtgga
     2761 cttgattatc gtcaagcacg cgcctatctt tcgtcctatc aaggacttgc ttgccagccg
     2821 tccgcaaaat cagatttaca tcgacctgat taagcatgat atcgcagttt atgtcagcca
     2881 taccaatatt gatatcgttg aaaatgggct caatgactgg ttttgtcaga tgctaggaat
     2941 cgaggagacg acttatctgc aggaaacagg tccagaacgt ggaattggac gtattgggaa
     3001 tattcagcct cagacatttt gggaattggc ccaacaggtc aagcaagtct ttgacctaga
     3061 tagccttcga atggtgcatt atcaagagaa tgatttgcag aagcctattt caagagtagc
     3121 aatttgtggt ggaagcgggc agtctttcta taaggatgct ttggcaaagg gggcagatgt
     3181 ctatatcact ggcgacatct actaccatac tgctcaggat atgctgtctg atggcttgtt
     3241 agcattggat ccaggtcact atatcgaagt gctttttgtg gaaaaaatcg cagcactcct
     3301 tactcaatgg aaagagaaaa aaggctggga actagagatt ttacctagtc aagcatcgac
     3361 caatcctttc cgtcatatct agttagaaag tgaagacaat gaaaaaagtt gccattattg
     3421 gagcaggaat tgtgggagca acagctgcct actacctctc gagagaaagt gacctagagg
     3481 tgaccgtttt tgaccatgag caaggtcaag ccaccaaggc cgcagcagga attatcagtc
     3541 cttggttttc caaacgccgt aataaagcct ggtacaagat ggcgcgcttg ggggctgatt
     3601 tttatgtgga tttattagct gatttagaga aatcaggaca aaaaatcgac ttttaccagc
     3661 gttcgggagt ctttctcttg aaaaaggatg aatccaagtt ggaagaactt tatcaactgg
     3721 ccctccagcg cagagaagaa tctcccttga tagggcaatt agccattctg gaccaagctt
     3781 cagctaatga attattccct ggtttgcagg gatttgaccg cctgctctat gcttctggtg
     3841 gagcgagagt agatggccaa ctcttggtaa ctcgtctgct agaagccagt catgtcaagc
     3901 tggtcaaaga aaaagtgact ctgacactct tatcatcagg ctaccagatt ggcgaagagg
     3961 tgtttgatca ggttattttg gcaacgggag cttggttggg ggacatgtta gagcctttag
     4021 gttatgaagt ggatgtccgt cctcaaaaag gacaactacg agattatcag cttgcccaag
     4081 acatggaagc ttaccctgtt gtcatgccag aaggggagtg ggatttgatt ccctttgcag
     4141 gtgggaaatt atccttgggc gctacccatg aaaatgacat gggatttgat ttgacggtag
     4201 atgaaccctt gctccaacaa atggaggagg cggccttgcc tcattaccca gttttagcta
     4261 gagcgacttc aagggctgag cgtgtgggaa tccgtgccta taccagtgat ttctctccct
     4321 tctttgggca ggtgccagaa ttggcaggtg tctatgcagc cagtggacta ggttcatcag
     4381 gcctcacaac tggtcctatc attggttacc atctagccca actgatccaa gacaaggagt
     4441 tgaccttgga ccctctaaat tacccaattg aaaactatgt caaacgagta aaaagcgaat
     4501 aatactcaat gaaaatcaaa gaacaaacta ggaaactagc cgcgggttac tcaaagcact
     4561 gctttgaggt tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgtgg
     4621 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtagataga actgacgaag
     4681 tcagtaacca tacccatggc aaggcgacgt tgacgtggtt tgaattggat tttcgaagag
     4741 tataagcatt ttactgaaat tttatcaggt agtttaggat ggcaaatgac attccctgtc
     4801 aaaaatggta aaataagaaa aaataatctg agaatcgagg aaaaaagatg caagaaaaga
     4861 ttttggtaac tggtggtgcc ggttttattg gaacccacac tgttattgaa ttgatccaag
     4921 caggccatca agttgttgtg gtggataatc ttgtcaatag caatcgtaag agtttagaag
     4981 ttgttgaaag aatcacagga gttgagattc ctttctatga ggcagatatt cgtgacacag
     5041 ataccctcag agatattttc aagcaggaag aaccgactgg tgtcattcac tttgctggtt
     5101 tgaaggctgt tggcgaatca acacgtatcc ctcttgccta ctatgacaac aatatcgctg
     5161 gaactgtcag ccttttgaaa gcaatggaag aaaacaactg taaaaacatc atcttcagtt
     5221 cttctgcgac agtttacggg gatccgcaca cagtgcccat cttggaagat ttcccacttt
     5281 cagtgaccaa cccatacggt cgtactaagc tcatgctaga ggaaattttg actgatattt
     5341 acaaagcaga ctcagaatgg aatgttgtct tgcttcgtta ctttaaccca atcggagccc
     5401 atgagagtag tgatttggga gaaaatccaa acggtattcc aaacaatctc ttgccatatg
     5461 tgactcaagt agccgttgga aaattagagc aagtgcaagt gtttggagac gattacgata
     5521 cggaagatgg aacaggtgtt cgtgactata tccacgttgt cgatttggct aagggtcacg
     5581 ttgcagcttt gaaaaaaatc caaaaaggtt caggactaaa cgtttataac cttggaactg
     5641 gtaaaggtta ctcagttctt gaaattatcc aaaacatgga aaaagcggtg ggacgtccta
     5701 ttccttaccg catcgtagaa cgtcgcccag gtgatatcgc tgcctgctac tcagacccag
     5761 caaaagctaa agcagaactc ggttgggaag cagaactcga catcacccaa atgtgtgaag
     5821 acgcatggcg ttggcagagc aagcatccaa atggatttga agactaagat gatgatttca
     5881 atcatcgtcc cttgtttaaa cgaagaggaa gtacttcctc ttttttatca ggctctggaa
     5941 gctttacttc cagatttgga aacagaaatc gagtatgtct ttgtcgatga tggatcaagt
     6001 gatgggacct tggaactctt aaaggcctat cgggagcaaa atccggcagt ccattatatt
     6061 tctttctctc gaaattttgg caaagaagca gccctttatg caggcttgca atatgcgaca
     6121 ggagatttgg tggtggtgat ggatgcagac ctccaagatc ctcctagtat gttgtttgag
     6181 atgaaaaatg tactagacaa aaatgtagac ttggactgcg ttgggacacg gagaactagt
     6241 cgggagggag aacccttctt tcgcagtttc tgtgctgttc tcttttatcg cctcatgcaa
     6301 aaaatcagcc cagtagctct gccgtcgggt gtccgtgact ttcgtatgat gagaaggtct
     6361 gtggtcgatg ccattttaag cttgactgag tccaatcgtt tttctaaggg actctttgcc
     6421 tgggtcggct ttaaaaccca ctatctggac tatccaaatg tcgaaaggca ggctggcaag
     6481 accagttgga gttttaggca actctttttt tactccattg aagggattgt taatttttca
     6541 gatttccctt tgactatagc ctttgtagct ggtctcctat cttgttttct ttctctgctg
     6601 atgacctttt ttgttgtggt tcggaccctc attttgggca atccgacatc tggttggacc
     6661 tctctgatgg ctgttattct ctttcttgga ggcattcaac tcttgaccat tgggattctc
     6721 ggtaagtata tcagtaagat ttatttagag actaaaaaaa gaccacttta tcttatcaaa
     6781 gaaaaaagtg accttcctga ttttacagaa aaaataaagt gaaaagacta taattttaca
     6841 tggaaatgtg ctaaactaga gggagtggat tatcgccatt gatttaggct tcctggtttc
     6901 caatcagggc gcgagttggg caatttttaa cagcctctaa gacatcctga ctaggagaaa
     6961 tttctttttc cagttggtca gggtcatcgt aaaaacgcac gattccatta tcgtggtaat
     7021 caaataaatc agaataagtt tggcaaagtc cacaggcgat acatcgttca ggtataagtg
     7081 tgattttcat gttcatattt atattgtaat aagaaaagta aaaaaaaaca aggagtaagg
     7141 tatggcaaaa gaaccgtggc aagaagatat ttatgatcaa gaagaatcaa gagcagggcg
     7201 tcggcatcga aaccacggag gggctgatag gatggctaat cgtattttga cgatcctagc
     7261 tagtattttc tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg
     7321 ggggagcaat cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca
     7381 aatctcatct tcaagcagtt ctcagcctga gcagagttca gagccagaat ctacttctag
     7441 tagttcagaa gaagctgcta atcctgaagg aacgattaaa gttctcgcag gagaagggga
     7501 agcagctatt gccgctcgtg caggaatctc cattgctcag ttagaggcct tgaatcctgg
     7561 gcacatggct acaggatctt ggtttgctaa tccaggtgat gttataaaaa taaaatagga
     7621 gccgatatga aaacaattca aattgctatt gatggtcctg cttccagcgg taagagtacg
     7681 gtcgcaaaga ttattgctaa ggattttgga ttcacctacc ttgatacagg agctatgtat
     7741 cgtgcagcga cctatatggc tcttaagaac caattaggag ttgaagaagt cgaagccctt
     7801 ctagccttgt tggaccagca tccaatcagc tttggacgtt cagaaactgg agaccagctt
     7861 gtttttgtag gagatgtgga tattacccat cctatccgtg aaaatgaagt gaccaatcat
     7921 gtttctgcta ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa
     7981 attgcccagc aaggcgggat tgtcatggat ggtcgcgata ttggaactgt tgtattgcca
     8041 caagcagaat tgaaaatttt cctagtagct tctgttgatg agagagcaga gcgtcgttac
     8101 aaggaaaata ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct
     8161 gcgcgtgact acaaggatag tcatcgtgag acttctcctc tcaaacaagc agaggatgct
     8221 gtctaccttg atacaactgg tttgaacatt caagaagtag ttgaaaaaat caaagcagaa
     8281 gctgaaaaaa gaatgtaaat gtagaaaagc cgatagattg atatcggttt ttttctaaat
     8341 ttgtcaaaat gctgatttta gggtataata gttgctgttc gagaaatttt gattttcaaa
     8401 gaatagtgaa tgatgataag gagaaaccat gaacaattta ccaaattgtc caaaatgtaa
     8461 ctcagagtat gtctacgaag acggtgccct actggtttgc ccagagtgtg ctcatgagtg
     8521 gaatcctgct gaagttgcag aagtagaaga aggtcttgtc gctatcgatg ccaacggaaa
     8581 taaattgact gatggtgata cagtaactct catcaaggac ttgaaagtaa aaggtgcgcc
     8641 aaaagatttg aaacaaggga cgcgcgtgaa aaatatccgc atcgtagaag gcgatcacaa
     8701 tatcgactgt aagattgatg gttttggtgc catgaaactt aaatcagagt ttgtgaggaa
     8761 gatttaagca tgtcaaaaca ctataaactt gtattttata gccgtatctt cttgtttcta
     8821 gcggctttta cgggagttta tcttgaaatt actaggcatg gtggttttgg gatgcttctc
     8881 tattacacgg ttctgtccaa cctcttggta actattttta ccctttatct tctaaaggtt
     8941 atgagccgtg taggtgaaaa ttggcaaagg ccaagtctct tgcgcttaaa aggtggggtc
     9001 accatgagta tcatgattac ctgtgtgatt taccatttcc tcttggcgcc cattgcgact
     9061 aatttctata ccctagaaaa tttcctttgc cactatatcg ttcccatctg gtttttagcg
     9121 gataccctct tttttgacaa acagggtcaa tacaagattt gggatccagc agtgtggacc
     9181 attttaccct ttctgtatat gatgtttgct ctttttaatg gcttggttct aaaactcaat
     9241 attccaaatg ccaaggataa tcctttccct tacttctttt tgaatgtgaa caagggttgg
     9301 aatgttgtgt ttaagtggtg tctgattatc tttgttgcct atatggtagc aggatttatt
     9361 ttctacttta tcaagcaaat caagagaaag tcatcctaat actcttcgaa aatctcttca
     9421 aaccacgtta gtttccatct gcaacctcaa aacactgttt tgaacaacct gcggctagct
     9481 ttctagtttg ctctttgatt ttcattgagt atgagataca agggattcat tttcgaattt
     9541 aagaaggagt ctacaaacca atgaggcttc ttcttttctt gcttatttga taactatcaa
     9601 aaaagtagaa gattaagaga aatatagaaa gagatttcat gaaacaattt ttagaacggg
     9661 ccagcatttt ggctctctcc ctcgttttga ttacctcctt ttccatttcg agtgccctac
     9721 cagccatgtt tgactattat cagggttatt ctaaggaaca aattgagctc ttggtgagct
     9781 tgccttcctt tggaatcatg atgatgttac tgctaaatgg tttcttagaa aaaatatttc
     9841 ctgagcgctt acagattagt ttgggcttgc tgattttatc attgagcggt acagctccct
     9901 tctggtacca agcctatccc tttgtctttg gaacacggct tctctttggt ttgggtcttg
     9961 ggatgatcaa tgccaaggcc atttctatta tcagtgaacg ctaccaagga aaaaggcgaa
    10021 ttcagatgtt agggctacgc gcttctgcag aggtcgttgg agcttctctc attaccttgg
    10081 ccgtcggtca gttgttggcc tttggttgga cagctatctt tctagcctat agtgctggat
    10141 ttttggtgct gcccctttat ctgctctttg tcccttatgg aaaatcaaag aaagaagtca
    10201 agaaaagagc gaaggaagca agtcgtttaa ctcgagaaat gaaaggctta atttttacct
    10261 tagctatcga agcggcagtt gtagtttgta ccaatacagc tattaccatc cgtattccaa
    10321 gtttgatggt ggaaagagga ttgggggatg cccagttatc tagttttgtt cttagtatca
    10381 tgcagttgat cgggattgtg gctggggtga gtttttcttt cttgatttct atctttaaag
    10441 agaaactgct cctctggtct ggtattacct ttggcttggg gcaaatcgtg attgccttgt
    10501 cttcatcctt gtgggtggta gtagcaggaa gtgttctggc tggatttgcc tatagtgtag
    10561 tcttgacgac ggtctttcaa cttgtctctg aacgaattcc agctaaactc ctcaatcaag
    10621 caacttcatt tgctgtatta ggctgtagtt tcggagcctt tacgacccca tttgttctag
    10681 gtgcaattgg cttactaact cacaatggga tgttggtctt tagtatctta ggaggttggt
    10741 tgattgtaat ctctatcttt gtcatgtacc tacttcagaa gagagcttag gattgattcc
    10801 taagtttttc ttttgatact caatgaaaat caaagagcat agttgattga gtttggaata
    10861 gtacgctgta gctgctaaaa tatttctaga aattaatttg acttttctaa tagagttatt
    10921 catatcttat ttcaatctac tatagaaagc tagccgcagg ttgctcaaaa tagtgttttg
    10981 aggttgcaga tggaaactga cgtggtttga agagattttc gaagagtatg agatttttat
    11041 acccatacaa tatttgtttt aggtccttgt ttacagacta atttcttgat aaaatagagg
    11101 ttatgacgag atataaagca actatttcct atgatggtta tgcctttgct ggctttcagc
    11161 gccagtctca tgcgcgtagc gttcaggaag aaattgaaaa aaccttgacc agattaaata
    11221 aagggcaaac cattactgtt cacggtgctg gtaggacaga tagtggggtt catgccctgg
    11281 gacaggtcat tcattttgac ctgccttatc agatggatga ggagaaactc cgttttgcct
    11341 tggataccca gtctcctgaa gatattgatg tgatttcgat tgagcttgtg gcagatgatt
    11401 ttcattgccg ttatgccaaa catagcaaga cctatgagtt tattgtggat agaggacgtc
    11461 ccaaaaatcc gatgcgccgt cactatgcca cccactttcc ctacccactc gatgtggaac
    11521 gaatgcagat tgcaatcaaa aagctagagg gaacccatga ttttaccggt tttacagcct
    11581 ctgggactag tgtagaggat aaggttcgca ccatcacaga agctagttta atagtcgatg
    11641 agacaggaca atttttgacc tttacctttt caggaaatgg tttcttgtat aaacagattc
    11701 gcaatatggt ggggactctt ctcaaaatcg gtaacaaccg catgccagta gagcagattg
    11761 atctcatctt ggagaagaag gacaggcaac ttgcaggtcc cactgcagca ccaaatggtt
    11821 tgtatttaaa ggagattcgt tatgaagaat aatcgtattt tagcactttc tggaaatgat
    11881 atttttagtg gtggtggact gtcagctgat ttggctacct ataccttgaa cggcttgcat
    11941 gggtttgtag cagtgacttg tttgacagcc ttgacagaaa aaggatttga agtctttcca
    12001 actgatgata ccatttttca acatgaatta gatagcttgc gtgatgtgga atttggggga
    12061 attaagattg gtcttctccc tactgtcagt gtggctgaga aggccttgga ctttatcaaa
    12121 caacgcccag gagtacctgt ggtgttggat cctgtcttgg tctgcaagga aacgcatgat
    12181 gtagctgtca gtgagctctg ccaagagttg attcgctttt tcccttatgt cagtgtgatt
    12241 acgcctaatc tcccagaagc agaattatta tccggtcagg aaattaaaac cttggaagac
    12301 atgaaaactg cagcgcagaa attgcatgat ttaggagcgc cagcagtcat tatcaagaga
    12361 ggcaatcgtc ttagtcagga caaggctgtg gatgtctttt atgatggaca gacctttact
    12421 atcctagaaa atccagttat ccaaggccaa aatgctggtg caggttgtac ctttgcctct
    12481 agcattgcca gtcacttgat taaaggtgat aaacttttgc cagcagtaga aagctctaag
    12541 gctttcgttt atcgtgctat tgcacaagca gatcagtatg gagtaagaca atatgaagca
    12601 aacaaaaaca actaaaatcg cccttgtatc cctattaacc gccctttctg tagttctagg
    12661 ttatttctta aaaatcccaa cacctacagg aattctaact cttttagatg ctggtgtctt
    12721 ctttgcggcc ttttactttg gtagtcgtga aggagcggta gtcggaggac tagcaagttt
    12781 cttgattgac ctcttatcag gctaccctca gtggatgttc tttagcttgg tcaaccatgg
    12841 cttgcaggga tttttcgcag gatttaaaag aaaaagtcag tggttaggcc ttattttagc
    12901 aactattgcc atggtaggag gctacgcctt gggttctact ttgatgaatg gctgggcagc
    12961 agccctccca gaaattctac caaatttcat gcaaaatatg gtagggatga ttgtaggatt
    13021 tattcttagt caaagtatca agaagattaa gtaatactct tcgaaaatct cttcaaacca
    13081 cgtcagcttt catctgcaac ctcaaaccac tgttttgagc agcctgaggc tagctttcta
    13141 gtttgcactt tgattttcat tgagtataaa gaggttgagt aaaaagtatt tcaaataaga
    13201 aaaacgcata gtatcaggtg ttgaatgaac tgccccccaa aagttagaca gaaaaaatct
    13261 aacttttggg gtgttt
//
LOCUS       DAVMAF010000049        13074 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMAF010000049 DAVMAF010000000
VERSION     DAVMAF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.049"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            39..971
                     /locus_tag="VYO97_001459"
     CDS             39..971
                     /locus_tag="VYO97_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754237.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDDKFKDKATLENNVKEINKQIDFIKKVDETFKQKKLDE
                     TVKSLTDIADKFQKQIDVLKKEEEQKASDKAKEDKKKEESTSSQSSNSSSQASSSSFS
                     SYSGNSDYAPEQTYTADNSQSYSGSTGGDNSNYKAPAATDNGGNDGYILGHVTGADGS
                     QRDFYFNRSNGDVRDTSGNHVGNFGGGITDFRQR"
     gene            complement(1074..1751)
                     /locus_tag="VYO97_001460"
     CDS             complement(1074..1751)
                     /locus_tag="VYO97_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1754238.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            complement(1735..2274)
                     /locus_tag="VYO97_001461"
     CDS             complement(1735..2274)
                     /locus_tag="VYO97_001461"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1754239.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            complement(2286..3416)
                     /locus_tag="VYO97_001462"
     CDS             complement(2286..3416)
                     /locus_tag="VYO97_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1754240.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGIFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            complement(3484..4182)
                     /gene="dapD"
                     /locus_tag="VYO97_001463"
     CDS             complement(3484..4182)
                     /gene="dapD"
                     /locus_tag="VYO97_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1754241.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGSVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            complement(4412..5323)
                     /locus_tag="VYO97_001464"
     CDS             complement(4412..5323)
                     /locus_tag="VYO97_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1754242.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            complement(5388..7853)
                     /locus_tag="VYO97_001465"
     CDS             complement(5388..7853)
                     /locus_tag="VYO97_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1754243.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRVGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7995..9251
                     /locus_tag="VYO97_001466"
     CDS             7995..9251
                     /locus_tag="VYO97_001466"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1754244.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            complement(9329..11392)
                     /locus_tag="VYO97_001467"
     CDS             complement(9329..11392)
                     /locus_tag="VYO97_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1754245.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(11394..11672)
                     /locus_tag="VYO97_001468"
     CDS             complement(11394..11672)
                     /locus_tag="VYO97_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754246.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            11824..12672
                     /locus_tag="VYO97_001469"
     CDS             11824..12672
                     /locus_tag="VYO97_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1754247.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTITILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
BASE COUNT         4070 a         2941 c         2377 g         3686 t
ORIGIN      
        1 ttttaaagta attgcggaca ctatcaaagt aaaagattat ggagaacaaa tttgtagaat
       61 ttatcgaaaa caataaaaaa gtaatcattt catcagttgc agttggtgtt gtattggtat
      121 tagggtttgg atggtattca tataaccaac aacaagcaga acaacaagca aaaattgtac
      181 aattagaaaa agatagcaaa tcagacaaag aacaagttga taaactattt gaatcatttg
      241 atgcatcttc agatgaatct atttctaaat taaaagaact atctgaaact tcacttaaaa
      301 ccgatgcagg taaagactat cttaataaca aagtcaaaga atcatctaaa gcaattgtag
      361 attttcattt gcaaaaaggt ttggcttatg atgttaaaga ttcagatgac aaatttaaag
      421 ataaagcaac cttagaaaat aatgtaaaag aaatcaataa acaaattgat tttatcaaga
      481 aagttgatga aactttcaaa caaaaaaaat tggatgaaac tgtaaaatct cttacagata
      541 ttgcagacaa attccaaaaa caaattgatg ttctgaagaa agaagaagaa caaaaagcat
      601 ctgacaaagc taaagaagat aagaagaaag aagaatcaac ttcatctcaa tcttcaaata
      661 gttcatctca agcatcaagc agttctttta gttcatattc tggtaattca gattatgctc
      721 cagaacaaac atacacagct gataatagcc aatcttattc aggttcaact ggtggagaca
      781 atagtaacta taaagcacca gctgctacag acaacggagg aaatgatggc tatattcttg
      841 ggcacgtaac tggtgcagat ggatcgcaaa gagattttta ttttaaccgt tcaaacggag
      901 atgttcgtga tacaagcggc aatcacgtag gaaactttgg agggggcatt acagatttta
      961 gacaacgtta ataactattt tagggctgtg ttgtttcgaa tggttccaaa acacattaaa
     1021 agctactcat tttttaagta gcttttttct tattcaagtt tacatattag ttgtcacaac
     1081 cccattccct tgtagaaaag caaaatggcg agtcctacga acaagactac cgctcctaat
     1141 ctctggctgg tattatacat ccgtttttct cctctaactg gaaagataac tgctagaaat
     1201 gcgccaccaa ctgcaccacc gatatggcct gctaggctga ttcctggaat cagaacactt
     1261 ccaataatgt taaccacaaa aagtgtcaga taggattgcc ctagctgttg gatataagga
     1321 ttgcgagttg catagcgaag aacaataatc gcggcaaata gcccataaag agaggtagag
     1381 gcgcctgctg ctaaggattt aggactaaat acaaaaacaa agagattgcc catcattcct
     1441 gataagagat agagaaaaaa gaactgctta gagccgaaaa tctcctctac ctgccttcca
     1501 agataataaa gtgaaagcat attaacaatg aaatgttccc acccaatatg aacaaaaatg
     1561 gcagacaaga gacgccaaac ctgctcggga aagaggcgaa tagctggccc atacatggct
     1621 ccaaatcgaa ataatgtatc tgccctgtca aagtttccgc ctgcagtgac caacattagt
     1681 aaaaatacca aggccgtcac taagaggaag aaactcgtca cagggtaacg tctatcaaag
     1741 atttccttca tcaattaata cctcctgaac aggaatatca tggttttcag gtataaagtc
     1801 ctgaatttga caaggatata tcgtactcaa agtacgacca gaaaaatgtt ccaaatagcg
     1861 gtcataatag cctccaccgt atcctatccg atatcctttc gtcgtaaaag ccagaccagg
     1921 aacatgaatc aaatcaatct gagatgcatc caccacttcc acatctccct gtggctccag
     1981 taaggcaaag gcagttttta ccaactgttg cggatcatag accacaaagt ccatgcgccc
     2041 cttgggataa gttttgggta ttaaaacctt cttgccgtcc ttcagcgcct gctcaatcag
     2101 ttcccgcgtt tgaaactcat gagaaaaaga gaggtaggtt gcgatgacct tggcttcttg
     2161 gtagaaggga tggtgtaaaa gccgctcggt taaagcttgg tctatagcct gtttttgctc
     2221 ttgagatata gccttcattt catgcaagac ttgcttgcgt aattccgatt tcatagacaa
     2281 gccctctatt ctgctgcctt ctttttcagg aaactagaca ccgcagccac cccaatagct
     2341 aagacttctt ccttaggact catttgaggg tgatgaagag cgtagggact atcgataccc
     2401 agccaaaaca tcacgccatc aacctttgaa aggagataac caaagtcctc gcctgtcata
     2461 gcaggttcga tatcaatcaa ctcgattccg tctttttcgt caaagaagtc catcagttca
     2521 cgcgccaagg ctggattgtt ctcaacaggt aggtatccac cttgtttgag ttccacttcg
     2581 acttccatat caaaggcagc tgcaacccct tctgcaactg tttttaccct cttttgcacc
     2641 aagagactca tgtcctgtgt caaggcacga atagttccat gtaaaaaagc tgtgtctgtg
     2701 atgacattgt tggtggttcc agcttgaaaa atgccgaagg tcaccactgc tccctcgatt
     2761 gggttgacat tgcggctaac aactgactgc acttgggtca caaagtaact agccgccacc
     2821 aaggcgtcat tggcttcatg aggaaaagct gcgtggccac ctttgccttt gaaacggatc
     2881 ttcacctcgc aagttcctgc aaagagtgta tgagtattag tcgcaatctg gccgactttc
     2941 aaatctggac gaacatggag accatagaat tggtttggca accaatctcc aaaagcaccg
     3001 tcctcataca tgagcatacc accagcttca ttttcttcag caggctgaaa tagaaagagc
     3061 agattattct tgggttgctc ctcaagggcg cgctcaagac agcctaaggc aatggtcata
     3121 tgaaaatcat gaccacaggc atgcatgcga ccttggtgtt gagaagcaaa aggtagacct
     3181 gtttgttcga cgataggcag gccatcaata tctgtccgcc aaccaatggt tcgctccggc
     3241 tgacttccct gcaggtagac caaaatccct gtccgccaag tacgaatttg aacaaaatcc
     3301 ttgcccgtag tcaatttctc aatcacatcc agcaaataag cctgagtctt gaactcctcc
     3361 aagccaatct ctggtatctg gtgtaaatct cgtctagtct gaatcaaatc taacatctat
     3421 ctgtcctccg atatagcaga aagaggctgg aaaaagggtt ccgcctcttt tttactttta
     3481 caattacaag gtacgaagcg catcctctag cgctgttttt tgttgggttt gggcatcaat
     3541 ttctttgatg atacgagctg gaacacctgc tactaccacg ttttctggga catcttgggt
     3601 aacgatagct cctgcagcga caactgaacc actaccgatt tggactcctt cgataaccac
     3661 tgcattagca ccgataagaa cattgtctcc gacacggact ggttcagcac tagctggctc
     3721 aatcacacct gccaaaactg cacctgcacc aacgtggcta ttttttccaa cgatggcacg
     3781 gccaccaagg atggcaccca tgtcaatcat ggttccagca ccgatttcag caccgatatt
     3841 gataacagat cccatcatga taacagcatt gtcaccaatt tccacctggt cacggataat
     3901 agcacctggc tcgatacgag cgttgatagc acgcttatct agcaaaggaa ctgcagaatt
     3961 acgagcatct tgctcgacaa cataatcttg attttctacc aaaccttcaa gaagcggagc
     4021 cacgtccttc cagtctccga ataggacatt tcctagtttg acaacagagc taggcacagc
     4081 agttgcgagt tgcccctcaa aggttacttt gacactggtt ttcttttcag cattggcgat
     4141 aaattggata atttcttgag cgttcatttt tgtagcagtc ataggtgcct cctggttcgt
     4201 tataatgata cctattctac caaaaaaggc ctcaaatttg aaaccttttt gtctgttttt
     4261 aggtatgatt ttttagggat gtgagataaa acgtgctgtc ggtaaaagca agcccctacc
     4321 gatacaacct aaaaacactc ttcacaacta tctccccgtg ttttattgac tattctagta
     4381 tagcacactt ttagaattaa gaaaagactt tttatctact ttctttcgct tcttctatag
     4441 acaaaaatat catgggtaaa ataatcaagg ctatagctag aaggagggac caatccacta
     4501 ctaatcctaa gaacaaaaca ctcaagagag cagaagagag aggttcactg gcactgataa
     4561 cggcaaccac caaaggagaa accaaggaca cagccttcat ggaaatgaaa aaagcaaaag
     4621 ccgttccaaa gaaagcgata atgaggcaaa tcaagatact ccaaatatca agagtaaagg
     4681 aaagctgata aaccggcgag aggacattgc taaacaaacc ggccaaaatc atcccccacc
     4741 caaccgtagg aacaaaacca taacgcttag caaaaggttg gggcaagata acattaaaca
     4801 taacacccat ggcactcagc aaacctgtta taagagctag cggcgtcatg gataactgag
     4861 agagatctcc ctttgtcgcc atcaagcaaa cacccagcat ggcaaccaaa acatagaaaa
     4921 cagcgctttt tgacgctcgt ttttgataaa ccaagcgatt gtaaaagagg ataaagacag
     4981 ggctaataaa ctgtaaaata gttgctgtcg tagcatttga gtattctaca cagagataga
     5041 aaaaatactg gactgaaaaa atccccaaaa tagcataggc taaaaagggc aggtaatttt
     5101 tcttgtctcg ccaaatatct agcacttgcg attttaattg tattgcagac caaatgagta
     5161 caagactccc tgccagtgtc aaacgcatag aggtaatcca gcccgaagac acctgataat
     5221 gagtaaagaa gtactctcct aaaattccac agattcccca tattaagccg gataggagcg
     5281 aataaatttt tccgttaaca atctttttct gatactgatt cataccctta ttgtaacacg
     5341 aaaggaaaaa agtggcaatc cattgattgg ccacttcatt tagagaatta tcgtctcgcc
     5401 cttgaagaag aaggtcgtgt agtacttgag ttactgctat cgctagaact actacttgaa
     5461 ctgctggagc tggatggagt tggtagactc cccacaatac tagaccaagc attctgataa
     5521 tccgcatcac ttccgccaat agcaaagcga taacttgtcg ctggcgctcc tgacttatta
     5581 gcccaatagc tggtaacagt cgaacctgtg acctctacct cttttccttc aacagaaacc
     5641 ttccctggtt tttgacctgt tgatttcaag acttccgatt tcactacact aggatctaaa
     5701 gcaaagcgct cgtttcccca aatgcttggg gaagcttgct gaatcgcatt tactagatga
     5761 gccatgtaat tagagttatt agaataacct actctacgtg acaatgaatg attatcatca
     5821 tgcccaatcc agccacctag ggttaatcta ggtgtcgaaa gcatgagcca catattttcg
     5881 tcttggttgg ttgtaccagt cttcccaatc caatctgcat tagccagagt aggatttaaa
     5941 gaagtcaggt tagacttgaa ggttgttgtc acacgagagg atagaacttc tcgtagcaat
     6001 ccctgcataa tcgtcgcagt agcttttgaa tagacttgaa ccggtttatc ctgatactca
     6061 tacaccactc taccatctgc tgcttcaatc tttgaaatca catgcttctg atgataaact
     6121 ccattattag ctaaggtctg atagccattg gtatgctggg caactgtgac ttcaatacca
     6181 ccacccattg gcaagctctc aataccgtac tcaggaatct cgtaacccat cttttccata
     6241 taacccttga catcaacacc cttttcacga agcatacgat aggtccagta agcagggata
     6301 ttccatgaat agttcagagc ttctcccaag gtcatcattc ctgttccctt gctattagca
     6361 tacataatcg gattgccatt agcaaagttt gttggatagt tagatagaat cgtttcactt
     6421 cccatcaagc cctggtcaat agcaataccg taggccagca agggcttggt agtagaagct
     6481 ggcgaacgtt tggtatcaaa ggcatgatta ttttgatttt cttgataatt acgaccacct
     6541 acaaagccta gaatagcacc tgtttggttg tccatcaaga cattccctac ttctacacga
     6601 cctgttccat cgtctaaaag atagccataa tcagcaaccg cactttgcat ggcagaatga
     6661 attttctgat ctatggtagt agtaatctta taaccaccat tttcaatttc cttggctgcc
     6721 aaatctcgat aaaacttctg agttgcctca tttttcaact ccttagcgga gacattgtct
     6781 ctctgagcta gatagtcata catacgttct tgagcttctg ccaaagttgt aaagtataaa
     6841 tagtctcgtg aaattcctgt aaccgtgccc gatggtaaaa agtcctgttt aaggtcataa
     6901 tccttgtact gagaatactc gtctttgctt aatgcacctg tacgatacat actgtaaaga
     6961 actgccttag cccgtcttaa gccaatttct aggtcttcat cactcttcaa ctccccagta
     7021 ttttcataag gagagtaagt aatgggactc tgtggaagtc ctgctaaaaa tgctgcttga
     7081 ggaacagtca actgactggc atctacaccg aaaattccct cagctgcttg ccgagcccct
     7141 gcaatattct gtcccttatt atttcggcca aagggagcca cattgagata ggtcgttaaa
     7201 atctcatctt tattcatggc gcgttccaag gcaagagcat ccacaatctc tgccgcctta
     7261 cgagccaagg tcggcgcatc cccaacaacc tgctgtttaa ttagttgctg ggtcaaggtt
     7321 gaacccccac tagaggaacc caaacctaca aatttcccca aggtcgcacg aatcaccgcc
     7381 ttgggtacta cacccttatg ttctttaaag tgttcatctt ctgtcgcaat gatagccttc
     7441 ttcagatttt ccgaaatttg ctcagatgag atagaagtgc gcaacaaatc actctctatg
     7501 gaagcaatca ccgtcccgtc cgaataggta atctctgaaa tagaagagat gtccttgacc
     7561 tgattcacca attcttctgt ctgaggcacc cgaaccttgt caaataaggc cactccgtat
     7621 cccaaagcaa tcccagctcc caacattcct cctagaaaac cgagtacaaa gagtaagtta
     7681 aataaggctt ttatactcag taaaatagct gggaaaatgg ctgacttatc taaggtttta
     7741 gattttttgg tacttgaacc tttcttgcca ggtctagctg attttttatt tttttgtttt
     7801 tgctggaaaa attccagcat ttttcgtttt aattcattta attgattttg catggatttc
     7861 ctcactctat ctattatacc acaaaaggga aattttcaat aaaatagcca ctttcttccc
     7921 tattctgcta ggctattgcc caagtttgtg atacaatagg tagaaacaat aattttaaaa
     7981 aggagaaaag acacatgcac atttttgatg agctaaaaga tcgtggtttg atatttcaaa
     8041 cgactgatga agaagctttg cgtaaagccc tagaagaagg tcaagtttct tattatactg
     8101 gctacgatcc aactgctgac agccttcacc taggccacct tgtcgcaatc ttgacaagtc
     8161 gtcgcttgca actagcaggt cacaaacctt atgcgctcgt tggcggtgct acaggtctca
     8221 tcggagatcc gtccttcaaa gatgctgaac gtagtctcca aacaaaagac acagtagatg
     8281 gctgggtcaa gtctatccaa ggacaacttt ctcgttttct tgactttgaa aatggcgaaa
     8341 acaaggctgt catggtcaac aactacgact ggtttggcag catcagcttc attgacttcc
     8401 tccgtgacat tggaaaatac ttcacggtca actacatgat gagtaaggaa tctgttaaaa
     8461 aacggatcga aacaggaatt tcttacactg agttcgctta ccaaatcatg caagggtacg
     8521 acttcttcgt ccttaaccaa gaccataatg tcactcttca aatcggtggt tctgaccagt
     8581 ggggaaatat gacagctggt accgaattgc ttcgtcgtaa ggcggacaag actggtcacg
     8641 ttatcactgt tccactaatc acagatgcaa ctggtaaaaa atttggtaaa tcagaaggaa
     8701 atgccgtctg gctcaatccc gaaaagactt ctccatacga aatgtaccaa ttctggatga
     8761 acgtgatgga cgctgacgct gttcgcttct tgaaaatctt tactttcttg tcacttgatg
     8821 agattgaaga tattcgtaaa caatttgaag cagcgccaca cgaacgcttg gctcaaaaag
     8881 tcttggctcg tgaagttgtt acacttgttc acggagaaga agcctacaaa gaagcactta
     8941 acatcactga gcaactcttt gcaggaaaca tcaaaaacct ttctgtcaaa gagctcaaac
     9001 aaggacttcg tggtgtgcca aactaccaag tacaggcaga cgaaaacaac aatatcgtgg
     9061 aactgctcgt ctcatctggt atagttaact caaaacgcca agcccgtgaa gacgtccaaa
     9121 acggagccat ctacgtaaac ggcgaccgca tccaagacct tgactatgtc ttgagtgacg
     9181 ctgataagtt agaaaatgaa ctgactgtta tccgtcgtgg gaagaaaaaa tactttgtat
     9241 tgacttacta aactattcaa catttatcta taaacaaagg agttaacctc gagaaaggta
     9301 actccttttt gctgttaata actctcatct atctattttt aatagacagg ctacgcagga
     9361 caatgcgcaa ggttgttaga ttatgtaaga tagagagatt tgaaggactg aaccaattaa
     9421 ataagccaaa gccaatcaaa ctactattta cgacaacggt atcctgaata tttttcttga
     9481 tgagtgtttg caaagatgat gataacgaat ccaactcttg gaagaaatcc aaacgattat
     9541 ctaacaataa gatatcactc atctgcttag aaatatctgc actctcattc atcaccacac
     9601 cgatatctga tagagttaaa gccgctgagt cattcaatcc atctccgacc atcaaaatag
     9661 tgtgacctgc tttctgcagt ttctctacta actcaaattt cccatcaggt ttcaagtctg
     9721 tatagacctg atcaaagggc aaatctttga ctaattcctc tgtcctaatc aaggtgtctc
     9781 ctgttgccag aatcaatttt ttcccctgtg ccttaagttt atccaaggct gtttttgctt
     9841 cttttctcaa aggagtatga atgcagaaca ttccaatcaa ttcattttga taagccaaga
     9901 ataagagatt gtagtgactc ttgtactctt caattaaagc attttgttct gaactgatat
     9961 gaatctgctc atcctgcatc aagacataat tcccaataag aactggttgg ccatctatat
    10021 gagatttgat ccccttgctt gcgatatatt ggagtttccc atgcatttcc tcatgttcaa
    10081 ttccctctat ctcagcttgc ttgacgatgg cattagcaat aggatgataa atgtgttcct
    10141 caagacaggc actgattctg agaatatctt cctcactata gtctccaaaa ggtaacacct
    10201 tttcaactat aggataacta gttgtgattg ttcctgtctt atcaaacaag aaagtatcaa
    10261 cttccagata tttctccaga acatctccat ccttaatcac catttcacgg ttcaaccctt
    10321 ccttgataac tgtcaaataa gctacaggag tagagatttt caaagcgcag gagaaatcga
    10381 ccaataggaa agaaatagcc ttagaaaaag aacctgtcaa taggtaagtc agcccagccc
    10441 ccaagaaatt atatttgacg actttatccg ccatcttgat gaaatagcgt tgtttcgttt
    10501 tcttgttttc ttcagatttc ttcatcaact caatcagctg taaaatacgg ctgttcatct
    10561 gattatctgt tacacgaatg cgtaactctc cagtttctaa tactgtattt gcacaaacca
    10621 aatcagactc tcttttttca actggaaaac tctctcctgt caaggaactt tcgttgacca
    10681 tacctaaacc tgaaactact tgtccatcaa acagaatttc atttccttga gataagatca
    10741 agacatctcc tatttgaaca tcggaactct tgatactaac aaccgtatcg ccctgtacta
    10801 ggaatacatc gctctctttt gcaagaagac tctgttctaa atctgttgca gtttttttca
    10861 aggaccactg atctaaatga ttccccaaat caagcataaa catgatattg ctagctgtct
    10921 ttgattggtt cataaacaaa gacaataaaa tagccgaaca gtccaagact tccatcgtta
    10981 gttccttacg cgctagtgtt tgataggctt ctctaatata acccaaagcc tgataacaag
    11041 tccatatata gcgaatagga tacggcacaa aactacgaaa aagtacacgc ttaaccgctg
    11101 cacctgaaac aatagaataa gcactctctt ctctacgaat gggaagagtc atcaactcag
    11161 aaactttccc tttatcaatt ctttttaaaa aggcttctgc attatctaat acagaaaagc
    11221 cttcttttat gcgtagagta aagtgctgtt gatccatgta aaactggata gactcaatcc
    11281 ccttttcatc tctcgccaag gaacgaagat agtcttgaat atccaaggta agtgaaaaag
    11341 aagatgatag tcggatatgt tggtatcctc tatgtagcac tttaaaagac atattattca
    11401 cctataaggc tatctaattg ctcttctttt ttctcttgct cgtacaaata tttggcatct
    11461 tgcaagacat cgtctccatg ttgcttcaca acagaaacag atgcatctag ctcgtctttc
    11521 aacttgtaag ccttagccaa agctttagaa taaccttttt tagcttcctt acttgctaag
    11581 attttcaaac caagggtacc aaatgcgaca ccacccaaaa ataatgaaga ttttttcgca
    11641 acttttgcaa cggttaatac ttcttttaac atacttattt cctccttatc attattatat
    11701 agcaatttag atataaaagc aatttcattc tataaattgg agaattagtt ttatttctac
    11761 tgaatctccc atctacttat tcctaaagtt gctttcattt gcctcttttg atacacttaa
    11821 actatgaata caaatctcaa gcccaaactt cagcgttttg cttctgcgac tgcctttgcc
    11881 tgtcctatct gtcaagaaaa tctgactctg ttagagacta atttcaagtg ctgcaaccgt
    11941 cattcttttg acttggcgaa atttggctat gtcaatctag cacctcaaat caagcaatct
    12001 gctaactatg acaaggaaaa ttttcaaaac cgtcaacaaa tcctagaagc cggcttttac
    12061 caagctatct tagatgctgt atctgacttg cttgcaagct caaaaactac cataacaatt
    12121 ttggatatcg gttgtggtga aggattctat tctcgcaaac tacaagaaag tcactctgaa
    12181 aaaactttct atgcctttga catctccaaa gattcagtcc aaatcgctgc taaaagtgaa
    12241 cccaactggg cagtcaattg gttcgttggc gacttggcac gccttcctat aaaagacgct
    12301 agcatggata tcctgcttga tatcttctca cctgccaact atggagaatt tcgtcgcgtt
    12361 ttatccaaag atggtatctt gataaaggtt atcccaactg aaaatcacct caaagaaatc
    12421 cgtcaaagag tacaggacca gctgacaaac aaggagtatt ctaaccaaga tatcaaggag
    12481 catttccagg aacactttac catcctatct agtcaaactg cctctctgac taagactatc
    12541 acagcagaac aactccaagc cctactcagt atgactcctc tcctctttca cgttgaccag
    12601 agcaagattg actggagcca actgacagag attaccatcg aagcagagat tttggttggg
    12661 aaagcattct aatactcttc aaaccacgtc aacgtcgcct tgccgtacgt atggttactg
    12721 acttcgtcag tttcatctac aacctcaaaa ccatgttttg agctgacttc gtcagtttta
    12781 tctgcaacct caaagctgta ctttgagcaa cctacggcta gtttactctt taattttcat
    12841 tgagtataaa ctagacttcg caaaagtaga aaacttaccg aaaatcttca atatgacaaa
    12901 aacgtataag aacaggtaca caaaccttaa tcttatgcgt tttgttatag tttccataca
    12961 cattaagggg aaatagttga aatcagttct tttaataacc attgaatacc atatactaca
    13021 tttaccccaa atatgatata ggccgtataa atcaagtatt tcgatttttc ccgc
//
LOCUS       DAVMAF010000050        13069 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMAF010000050 DAVMAF010000000
VERSION     DAVMAF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.050"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            109..1455
                     /gene="celB"
                     /locus_tag="VYO97_001470"
     CDS             109..1455
                     /gene="celB"
                     /locus_tag="VYO97_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1754248.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            1834..2010
                     /locus_tag="VYO97_001471"
     CDS             1834..2010
                     /locus_tag="VYO97_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754249.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2411..4624
                     /locus_tag="VYO97_001472"
     CDS             2411..4624
                     /locus_tag="VYO97_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEV1754250.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFVQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDEADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETENVPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYKLEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4846..5322)
                     /locus_tag="VYO97_001473"
     CDS             complement(4846..5322)
                     /locus_tag="VYO97_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1754251.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5545..8745
                     /locus_tag="VYO97_001474"
     CDS             5545..8745
                     /locus_tag="VYO97_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1754252.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPFSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9125..9510
                     /gene="tnpA"
                     /locus_tag="VYO97_001475"
                     /pseudo
     CDS             9125..9510
                     /gene="tnpA"
                     /locus_tag="VYO97_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9889..10518)
                     /locus_tag="VYO97_001476"
     CDS             complement(9889..10518)
                     /locus_tag="VYO97_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1754253.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(10528..11529)
                     /locus_tag="VYO97_001477"
     CDS             complement(10528..11529)
                     /locus_tag="VYO97_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1754254.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISALEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKETYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLSGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11560..12201)
                     /locus_tag="VYO97_001478"
     CDS             complement(11560..12201)
                     /locus_tag="VYO97_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1754255.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPKERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12220..13035)
                     /locus_tag="VYO97_001479"
     CDS             complement(12220..13035)
                     /locus_tag="VYO97_001479"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1754256.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4074 a         2235 c         2791 g         3969 t
ORIGIN      
        1 aattggaaaa gaactcactt agtttataat cctgatctag tataatttgt atttgaaact
       61 agtctttaga aagtaaaaat tattatgttt tattaaggag gtctatttat ggatcaacaa
      121 aacgggttgt ttggttttct tgaaaaccat gttatgggac caatgggcaa acttgctcag
      181 tttaaagtag tgcgtgctat cacggctgca ggtatggctg ctgtaccatt tactattgta
      241 ggatcaatgt ttttggtatt cagtattttg ccacaagctt tctcattttg gccaattgtg
      301 gcagatattt tctctgcttc atttgataaa ttcacatcac tttacatggt tgcaaactat
      361 gcgactatgg gttctctatc tctttatttc gttctatcac ttgcatatga attgacaaaa
      421 atttatgcag aggaagaaga actcaatatg aatcctctta atggtgcctt gctcgccttg
      481 atggcttttg tcatgacagt accgcaaatc atttttgatg gtggaatgat gaagactgtg
      541 acaagtctaa aagaaggtgc agtaattgca gatggatggg caatgggaaa tggagtcgca
      601 cgttttggga caacagggat ttttaccgca atcattatgg caattgtgac tgttcttatt
      661 tatcgtatgt gtgttaaaca taattgggtt attaaaatgc ctgaagctgt tccagaagga
      721 gtttctcgtg gatttaccgc tttggttccg ggatttgttg ttgcatttgt tgttatcttt
      781 atcaacggtc ttcttgtagc aatgggaaca gatattttta aagtcattgc aattccattt
      841 ggttttgtat ccaatctgac taattcgtgg attggtttaa tgattattta tctattgact
      901 caactacttt ggattgtagg tatccacggt gcgaacattg tttttgcatt tgttagtcca
      961 attgctcttg ctaacatggc tgaaaatgct gctggcgggc acttcgctgt tgcaggtgaa
     1021 ttttctaata tgtttgtaat tgcaggtggt tctggtgcaa ctttaggact atgtttatat
     1081 attgcttttg cctctaaatc tgaacagctt aaagcaatag gacgagcatc tgtagttccg
     1141 gccttattta atattaatga accattaatt tttggattac ctattatcta taatccagcc
     1201 ttggctatac catttatttt agcaccaatg gttactgcta ctatttatta cgtagcgaat
     1261 tctctaaact ttattaagcc aattatcgca caggttccat ggccaactcc agtagggatt
     1321 ggagctttct tagggacagc agatcttcga gctgtattag ttgctctagt atgtgcattt
     1381 gcagcattcc tagtctatct tccattcatc cgtgtatatg atcaaaaatt ggtgaaagaa
     1441 gagcaaggta tctaataagt aaaaaagtat actcgatgat agaatccata tagtttaaat
     1501 aatgagtgtt aatattagca gtgaagaggt ctttttaaga tattcttctc tgctttttca
     1561 tattttttcg ctatgtaggg tatgaagcaa aatagaaagt agttcgaata gttgataaac
     1621 agggaactgc tgttatttaa aaaaacaata aagtaaagta tgttatcgta gaatacgata
     1681 caatagtaaa aaagcaaaaa gaatgcgttt tatatttggg tttgaggttt ataaaatagg
     1741 ataagcgtgc tgaaaactta gatatgacaa acaaaataaa atttttaaaa gctacagaga
     1801 aacatggtat aatgtatcta aaggaggtag catatgtcct tagatataga taaagaaaag
     1861 atgacaatca tgggaatagc ttttgaaaac cgatccgttt ttaagagtgt ttggtatgct
     1921 ttaagtacaa atatgattga aggatggcga cctacggtta gtgatgttga aaaattacga
     1981 gatgaagctc ttgctctggg gatgacttaa tgaagcgcaa ctgttatgaa tcttatgact
     2041 atgttgatcc ggataattta tataccgatc cgggttcttc agttctaaga aataagcagg
     2101 aaggaaaaaa gcacgcgagc ttgaatatcg tatgattgct agtaagagtt tgaaattatt
     2161 tatcaatcct atcttagtta atagtttaaa agtagtttgt ttcacaactg cataagctta
     2221 gaaaagttga aaagtgataa ctctttaata aactcttggt ttgttaaaag atattttgtc
     2281 cactacattt tttcaattga ctattatttg tcatagatat aacagataga acattgcatt
     2341 cagaataatg aaaaacgcat caaaaatggt gcgttctgtt tttatgctac cataaaagga
     2401 gggaaacaaa ttgaaaattt ttaagggaga gttttatcga atctctgtat taacagacaa
     2461 gttagtaagg ttagaatact ctcaaactgg aagttttgag gatagaacga cacaacttat
     2521 ctataataga gattttgtcc aagtttcgtt agattatatc gagacatcaa acgtactaga
     2581 tattatgacg gactattttc atttgcattt taataaagga gaatttaacg ccgaaaattt
     2641 atttatagaa ttaaaaggaa attttgccgt atatggtagt cgctggtatt ttggtgaatc
     2701 tattgaaacg ttaaaaggaa cagctcggac tctggatgag gcagatggag caatctcgtt
     2761 agaagatgga attattagcc gaaatggtat agccttattg gatgattctc aaggatttat
     2821 ttgggatgaa cagtctggtt atattgagag agaaaatcaa attgatctct atttctttgc
     2881 ctatgggcat gattatagag gagcaatcag agacttttac catttgactg gttcaacacc
     2941 cttgttgcca agatatgctt taggcaattg gtggagtagg tattggcctt atacgtcaga
     3001 tgaatacttg gatttaatag acagatttga aacagagaac gttccattat ctatcggtgt
     3061 tttagatatg gattggcata taactgacat tccagctcgt tttggaagtg gctggacagg
     3121 atatagttgg aataaaaact taatacctaa tccagaacag ttattgcaac aacttcatga
     3181 tagaaagttg aaactctcct taaatgtcca tcctgctgat ggtatacggg cttatgaaga
     3241 agcttatcct caagtcgcaa aacgattggg gttaaatgta gaactagaag aacctgctat
     3301 ttttgatttt tttaatccat cttttagaga agcctacttt aaagatgttc attataagct
     3361 agaaaagcag ggagtagatt tttggtggat tgactggcaa caggggactc aaggcatgtt
     3421 ggatccactg tggcttttaa atcattatca ctatcaggat agttgtaaaa atgcagaagg
     3481 tggtttgatt ttatcaagat atgcaggtcc tggtagtcac cgctaccctg ttggtttttc
     3541 aggggacact attattagtt ggaattcctt aagatttcaa ccttatttta cagcgacagc
     3601 atcaaatatc ggttatagtt ggtggagcca tgatatcggt gggcacatgc tgggggatta
     3661 tgacgaagag ctgcaaacta gatggctaca gtttggtgtt tttagtccga tcactcgatt
     3721 acatagttct agaagtcctt ttaatagtaa agaaccttgg tttttttcag aaacaacatc
     3781 taagattatg aagaaatacc ttcgtttgag gcatcagatg attccatatc tatacactat
     3841 gaatgtaaag acacacgagg aaggtgcccc attaatcagc ccaatgtatt atttctaccc
     3901 agagaatgat gagagctata atgttccaaa ccaatacttt tttggaacag aactaatggt
     3961 ggctcccatt gtagaaaaga tggacttggc attccaatct gcaaaagtgg atgtatggtt
     4021 ccctgaaggt gaatggtatg acttcttttc agagaaaaaa tatacaggtg gtgtgaagtt
     4081 gagtgtttat agagacattt cgactatacc tgtgtttgca aaaagtggtg cgatcattcc
     4141 cttggttggt tctgagatag atatgggtgt tgatttacct gaagttgtag attggtatgt
     4201 attcccaggc aaacaacatt cttttgaaat gcttgaagat caaaatggtc aaagatataa
     4261 aacaagatta tcaatcgatt gggaaatggg gatggtagag ttaacattac aaggagattc
     4321 tagtatcgtt ccaagcaata gaaggcatag aattcatttt aaaggaacga atgtgtctat
     4381 aattgaatta ccaaataaga atgatacagc tagatttgaa tgtaaagata ataaaacaat
     4441 atctttaaat gatgaagttt ttagactact aaagacagct tctcttccat atgaattaaa
     4501 agatagattg ttaaatcaat tcatcaatgc gaaaaactct catgacttaa tgaatatctt
     4561 gcatcatcag gataaggaat tgagagggcg tttgttggaa atgatattta ctagccaaaa
     4621 ctaattgata taattagaaa gtcatttgtt tagtgtatat gaagattctg tatggtattc
     4681 gttatcactg gtcacattga aaatgtgaaa taagcaccta caacatagac tttacaacac
     4741 ttcatgtttc atatcatggg gtgtttttct atttaagtca aagccaatca aaaaatctca
     4801 ctattcatca ggttaaagga aacctaatca tagtgagatt gtgaattata gtagagtttg
     4861 tatcgcctct tcaatttgtt ttgggtctgt ttttgaagaa aagcgttcaa agacttgccc
     4921 atctcgaccg atgagaaact tagcgaaatt ccattcgatt cgttttccta gtgggccgga
     4981 tttctggtct tttaaccaga catagagagg gtctgcttcc ttaccgttga ccttaatctt
     5041 ggcaaaacgt gggaaggtgg tttgaaaatg taggctacag aaggcgttga tttcctctgc
     5101 gctgccgggt gcttgtccca taaactgatt gcaagggaaa tccaatattt cgaagccctg
     5161 atcttgatag cgttcataga gttcttgaag tccctggtac tggggcgtta aaccacatcc
     5221 agtagcagtg ttgacaatca agagaacttt gccacgatag ctatccaagg gagttgcttg
     5281 gttgttttgg ttcaaaacgg aaaaatcata aagtgaagtc atgagggcct cttttctttt
     5341 tctacattgt aacagttttt acttttttcg tcgagtcgga tggaagatat tgttgaagaa
     5401 taagccttca atatgataga aaccgtttac aaataacttt aaaaatggta tgatttaatt
     5461 aaaccggttt acttagttgc tataaatctc taatttgaga tttagttcct attgatttta
     5521 caatatgttt attggagtgt atacatgcaa acaaaaacaa agaagctcat tgtgagtttg
     5581 tcttcacttg ttttatcagg atttttatta aaccattata tgacagttgg agcggaagaa
     5641 acgactacga ataccattca gcaaagccag aaggaagttc agtatcagca aagggataca
     5701 aaaaatttag ttgaaaatgg tgattttggt cagacggagg acggaagcag tccgtggaca
     5761 ggaagcaaag ctcaggggtg gtcagcttgg gtagaccaga agaatagtgc agatgcctca
     5821 actcgagtca ttgaggctaa ggatggggct atcactatct caagccatga gaaattaagg
     5881 gcagcggttc accgtatggt tcctattgaa gctaagaaaa agtataaact gcgtttcaag
     5941 attaaaacag atcataaagt cgggattgcc aaagttcgta tcattgagga aagtgataag
     6001 gacaagcgat tgtggaattc tgcaacgacg tcaggaacaa aggactggca gaccattgaa
     6061 gcagactata gcccgacttt agatgttgat aaaatcaagc tggagttatt ctatgaaaca
     6121 ggaactggga ctgtttcctt taaggatatt gagctggtag aggtagcaga ccagccttct
     6181 gaggactatc aaacagataa acagcttgag gaaaagattg atttaccaat tggaaaaaaa
     6241 catgtttttt ctcttgcgga ctatacttat aaggtagaaa atcctgacgt tgcttcagtc
     6301 aaaaatggaa ttttagaacc tcttaaggaa gggacaacca atgtcattgt cagtaaagat
     6361 ggcaaggaag tgaaaaagat tcctttgaag attctagcct ctgttaagga tgcatacaca
     6421 gaccgtttgg atgactggaa tggcatcatc gctgggaatc aatactatga ttctaaaaat
     6481 gaacagatgg ccaaattaaa ccaggaattg gaaggaaagg tagctgatag cctatctagt
     6541 atttcaagtc aggcggaccg cacctatttg tgggaaaaat tttcaaatta taaaatgtct
     6601 gcaaatctga ctgccactta tcggaaattg gaggagatgg ccaagcaagt gaccaatcct
     6661 ttttctcgtt attatcaaga tgaaactgtc gttcgaacag tcagggattc catggaatgg
     6721 atgcataaac atgtctacaa tagtgaaaag agcattgttg ggaactggtg ggattatgaa
     6781 atcggtacac ctcgtgccat caacaatacc ttgtctctga tgaaagaata cttctctgat
     6841 gaggaaatta aaaaatatac agatgtgatt gaaaaatttg taccagatcc cgaacatttc
     6901 cgaaagacga ctgataaccc atttaaggct ctaggtggaa acttagttga tatgggaaga
     6961 gtaaaagtaa tagctggttt actgcgtaag gatgatcaag aaatttcttc taccattcgc
     7021 tcgattgagc aagtgttcaa gttggtagac caaggtgaag gtttttatca agatggatcc
     7081 tatatcgacc ataccaatgt tgcctatacg ggtgcttatg ggaatgtttt gattgatggc
     7141 ctgtctcaac tgttgccagt cattcaaaag accaagaatc caatcgataa agataaaatg
     7201 caaaccatgt accactggat tgataaatcg tttgctcctt tgctggtgaa tggagagctg
     7261 atggatatga gtcgtggacg ctcgatcagt cgtgcaaata gcgaggggca cgtggccgca
     7321 gtagaagtac taagagggat tcaccgaata gcggatatgt ctgaaggaga aaccaaacaa
     7381 cgtttgcaga gtcttgtgaa gaccattgtt caatcggata gttattatga tgtctttaag
     7441 aatttgaaga cttataagga tatcagtttg atgcaatcct tgttaagtga tgcaggagtc
     7501 gcaagtgttc caagaacaag ttacctatct gcctttaaca agatggataa aacagccatg
     7561 tacaatgcag agaaagggtt tggatttggc ttgtcactct tttccagtcg taccttgaat
     7621 tacgaacaca tgaacaagga aaataaacgt ggttggtata cgagtgatgg gatgttctat
     7681 ctttacaatg gcgatttgag tcactatagc gatggctact ggccaacagt taatccatat
     7741 aagatgcctg gtacaacaga gacggatgct aagagagcgg atagcgatac aggtaaagtt
     7801 ttaccgtctg ctttcgttgg aacgagcaaa ctagatgatg ccaatgcgac agcaaccatg
     7861 gatttcacca actggaatca aacattgact gctcataaga gctggtttat gctaaaggat
     7921 aagatcgcct ttttaggaag caatatccaa aacacttcaa cagatactgc tgcaactaca
     7981 attgaccaga gaaaactgga atcaagtaat ccatataaag tctatgtcaa tgataaagaa
     8041 gcctccctta cagaacaaga aaaggattat cctgaaaccc aaagtgtctt tttagaatca
     8101 tccgattcga aaaagaatat tggttacttt ttctttaaga agagttcaat cagtatgagt
     8161 aaggctttgc aaaagggagc ctggaaggat atcaatgaag gacagtcaga caaggaagtt
     8221 gaaaatgaat ttcttacgat tagtcaggct cataagcaaa atggagattc ttatggctat
     8281 atgctcatcc ctaacgtggg tcgtgccacc ttcaatcaaa tgataaaaga gttagaaagt
     8341 agcctcatcg aaaataacga aacccttcag tctgtttatg atgccaaaca aggagtttgg
     8401 ggcattgtga aatatgatga ttctgtctct actatttcca accaattcca agttttgaaa
     8461 cgtggagtct ataccattcg aaaagaaggg gatgaatata agattgccta ctataatcct
     8521 gaaacccagg aatcagctcc agatcaggaa gtctttaaaa agctagagca agcagctcag
     8581 ccacaagtac agaattcaaa agaaaaggaa aaatctgaag aggaaaagaa ccattcggat
     8641 caaaagaatc tccctcagac aggagaaggt cagtcaatct tggcaagtct agggttcttg
     8701 ctacttgggg cgttttattt attccgtaga ggaaagaaca actaatttgt tcatcatcta
     8761 gatgaattac atgaaactgt ggaaaataga aaaaatccga agaaggccaa atatcttcgg
     8821 atttttcttt taaaaaagga taagttgata cttatccttt ttacttcatt atgtgactat
     8881 agcgttttga aactagaata ggacatcata actgctaaaa gatttctata aattcatttg
     8941 attttcctaa tcaatttatt cgtatcctat ttcactctac tatattagtc cgtctcgcac
     9001 ctcacggagc gagacaaaac aaccacccgc tatgcgggtg cgcgtcgaag gttataccaa
     9061 aaaaactcca aacgcgatac aataaaggtg ttcaatccac ttgtaaagcg aaaggagaaa
     9121 aaatatggca caaaaggctc atagtttatc tcacacaaag tggaggtgtt ctatcacatt
     9181 gtgttcaccc ctaagtatag acgaaaagtc atctataatc aatatagaag tagtttaggc
     9241 gaaatatttc atcgcttgtg tagttataaa ggtgttgaaa tgatcgaggg tcacttaatg
     9301 ccagaccatg tacacatgtt agtcagtatt ccactaaggc taagtgtttc gagtttcatg
     9361 ggttatttaa aaggaaaaag tgcactcatg atgtttgaca aacacgccaa tctcaagtac
     9421 aagtttggga atcggcattt ctgggtggaa ggttattatg taagtccagt agggcttaat
     9481 gaagccacaa ttaagaaata tagtcaagat taaagaaatt atcgagtgga tgatttcttc
     9541 acgagtatga aaatttgaga actagtaaag tatgatatat agtaaaatga aataagaaca
     9601 ggacaaaatc gatcaggaca gtcaaatcga tttttaacaa tgttttagaa gtagaggtat
     9661 actattccag tttcaatcta ctatagcact agataaattg agtgtaaaag aatatgagga
     9721 tccctttagg gatagtggta agtaatacca aagtctctta aagaggcaag tgacgagtca
     9781 agagcaataa ggcttgaaca acgtgaaagc cagcgtcttt aggcgctggc tgatgatttg
     9841 ggcttatagc tctgggataa accacccgtt agacgggtag ttatgatttt atctgagtgt
     9901 aatatactgt tgggcaatct cgctgatgcg gtcaaagttg ccttgggaag cgagtttatt
     9961 gagttcgcca ccaattccaa cggcatctgc accagcagcg aaccattgag ggatgttgtt
    10021 taggccgact cctccggtta ccattacgga aacttgtggg atcggtgcct tgactgcaga
    10081 gatatatgct ggactgagag tactacctgg gaagagtttg atgatttcac taccggcttc
    10141 aagtgcagtc gtgatctctg tgagggtaat acagcctgga atgtacggtg tgctgtagag
    10201 attgcacatt ttcgcagttt cagcatggaa agatggagaa acaacgtaat ttgctccagc
    10261 tagaatggca tctctagcag ttacggcatc aagcacagta cctgcaccga tacaaacact
    10321 ctgatcgtcc tgatacaagt ctacaagttc cttgatgatt tgtcctgcat actgattggt
    10381 ataggcgatt tcaatagctt tgataccgcc cttgatacaa gcaatcgagg cttgtagtcc
    10441 ttcttccttt gtatttcctc gaatgacagc gacaattttc tgttttttta gttcaataat
    10501 cgtatctgat ttggtcatgt aattctccta acgaatgata tcttgtgcat ttgccagtaa
    10561 attttcaata ctagttgcgg aagtggagag atggtctcct gaaagagtgc atttgagagt
    10621 tgcgctcgca actgcaaagt caatggtagt ttttagggag gaatgatgga gtagttggta
    10681 aagggcacca gatataaagg catccccgct accaattcgt tgatagactg cagtttttag
    10741 ttggacagac tcttcaaatc gttcttccag agcataggct tgatagacat ttttgtcttg
    10801 ctcatcacta gatcggaggg tatggaaaat agccttgaaa ccataggttt cttttaaaag
    10861 tcgcatgcga ttttccacct cttctaggct agcactgtct cttggaaaca tctctagatt
    10921 ttggtcatca atcatgagag gatcaatacc gaagcaatag tcagtaaaac gtgcaaactt
    10981 agaaaattca tacttggctt ctagggctga aatcatcttt gttctcagat tgagatccat
    11041 tgaaacgaca attcctcggc gcttggcttc ttccaagagt aggagaagga tcgcacggac
    11101 ctcttgaccg atagctatgg tgattccact aaaatgaaaa tggctaattc cctgaaagag
    11161 agaatccata tctagcatgt ttggccgaat ctggctgata ctcgtatgct tacgatcgta
    11221 gaaaacttca ctttgacgac aaccaaagcc gttctccaaa tagtagaggc cgattcgatc
    11281 gccaagccga cagattgaac tggtatcgat ttggtgctgt ttcaagaatg tgagaaaacg
    11341 atctccaatc tcgttggcag ggagtgcggt aaagactttc gttgagatac ccagggcttg
    11401 caaattacaa gcgatgttaa tttctgatcc gccaaaataa gtcgaactgg caacatgatc
    11461 tccgatactg gtggcatcta atggtgagat tcgaattaat ggttcgccaa acagtaatat
    11521 tttcgtcatc cattagttcc tttctggttt agaatagctt tacttggcta atacttcttt
    11581 caaataagca gcgacttcat catcttggca gttttcaaag aagtattctt ggaaatattt
    11641 gccagagatg gtgtctttga ggaagtcttg gtcgattgtt ttaaggatgg tcatcaaatc
    11701 attgtgggtg atttgtttca cctcgtttaa gatacgagcg ttgcgttgtt cagggattac
    11761 acgttctttt gggtagccac cgcccatttc ttcagcaaat aagcgttcaa acatcaattt
    11821 gagggtcagt tctgcccccc agccaaatcc tttggcataa gggatagaca aggcgttacc
    11881 accattgatt tgagaataaa ggtaagcgtc agttgggtcc actgctagac cacagacaac
    11941 accagggaag ctgtttaaag caagcatagc ccctacaccc gtaccacagc cggtaacaac
    12001 aaagtcaact gcctttgtat ttaaaaggat ggcagccatt agtccgttct gcacataagt
    12061 taattgactt tctccttctt ctccacgcat accatagtta aataattggt agtctttttt
    12121 atctgtcgct tcttttagac tatcgtaaat aatgtgattc ttgctagctt gactattttc
    12181 attgattaat gcgattttca taagatcttc tttctatttt tactcaggtt gttttccgat
    12241 gtaggctaag ataccgccat ctacatatag gatgtggcca ttgacaaaat tgctggcatc
    12301 actagcgaga aagacagcag ggcccatcaa atcttcagga tttccccaac gtgcagcagg
    12361 tgtttttgca atgatgaact ggtcaaatgg gtggcgagaa ccatcttctt gcaactcacg
    12421 aagaggtgct gtttgaggag tggcaatata acccggtcca attccgttac attggatatt
    12481 ggctccaccg tattcagacg caatgttgcg ggtcaacatt ttcaaccccc ctttagcagc
    12541 agcataagcg ctaactgttt cacgtcccag ttcgctcatc atcgaacaaa tattgataat
    12601 ctttccatgc cctttcttta tcatagaagg aataactgcc tttgaaacga taaatggtgc
    12661 gtttaagtca atatcgatga ccttacggaa atcagcggcg ctcatttcgc acattggaac
    12721 tcggcggata ataccagcgt tattaacgag gatgtcaatg acaccaacct cttgttcgat
    12781 ttgcttgacc atggcttgga taccgtcctc gtctgtcacg tcacagacat atccatgggc
    12841 ttcgatgcca acttcacgat aagcttcaat ccctttattg accagatctt gattgatatc
    12901 gttaaagaca atagtagcgc cggcttcagc gtaggatttg gcaatagcaa atccaattcc
    12961 ataagaagcg ccggtgatga gagcaatttt tccttttaaa gaaaactgct caattgagaa
    13021 tgatgtattt gtcatttgat acttcctttc aaatttgtta agacttctc
//
LOCUS       DAVMAF010000051        12964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMAF010000051 DAVMAF010000000
VERSION     DAVMAF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.051"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            35..1244
                     /gene="sstT"
                     /locus_tag="VYO97_001480"
                     /pseudo
     CDS             35..1244
                     /gene="sstT"
                     /locus_tag="VYO97_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1312..2265
                     /locus_tag="VYO97_001481"
     CDS             1312..2265
                     /locus_tag="VYO97_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1754257.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2409..2789
                     /locus_tag="VYO97_001482"
     CDS             2409..2789
                     /locus_tag="VYO97_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1754258.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            2888..3109
                     /locus_tag="VYO97_001483"
     CDS             2888..3109
                     /locus_tag="VYO97_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1754259.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3126..3440
                     /gene="trxA"
                     /locus_tag="VYO97_001484"
     CDS             3126..3440
                     /gene="trxA"
                     /locus_tag="VYO97_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1754260.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3746..4279
                     /gene="queF"
                     /locus_tag="VYO97_001485"
     CDS             3746..4279
                     /gene="queF"
                     /locus_tag="VYO97_001485"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1754261.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQHHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            4329..4508
                     /locus_tag="VYO97_001486"
     CDS             4329..4508
                     /locus_tag="VYO97_001486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754262.1"
                     /translation="MLKAVFEVVDKTDEVSLKHCFEVADRTDEVSNHTYDKVKLTWFE
                     EIFEEYHTKKPCSSR"
     gene            complement(4533..5201)
                     /locus_tag="VYO97_001487"
     CDS             complement(4533..5201)
                     /locus_tag="VYO97_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1754263.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(5312..6103)
                     /locus_tag="VYO97_001488"
     CDS             complement(5312..6103)
                     /locus_tag="VYO97_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1754264.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLIFTSF
                     AKGIQEELFIQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLSADANQ
                     QSVFYQGLQSEIRDDLSNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(6528..7292)
                     /locus_tag="VYO97_001489"
     CDS             complement(6528..7292)
                     /locus_tag="VYO97_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754265.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(7285..7935)
                     /locus_tag="VYO97_001490"
     CDS             complement(7285..7935)
                     /locus_tag="VYO97_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754266.1"
                     /translation="MGILGINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSK
                     SCLGIVGQEDMLDMTLNVYDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVN
                     QLSSGMRRRLVLARALINRPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITS
                     HYMNEIEYLTDRVLMLHQGTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            8302..8979
                     /locus_tag="VYO97_001491"
     CDS             8302..8979
                     /locus_tag="VYO97_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1754267.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
     gene            complement(9039..10835)
                     /gene="pepF"
                     /locus_tag="VYO97_001492"
     CDS             complement(9039..10835)
                     /gene="pepF"
                     /locus_tag="VYO97_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1754268.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(10846..11589)
                     /locus_tag="VYO97_001493"
     CDS             complement(10846..11589)
                     /locus_tag="VYO97_001493"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1754269.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(11591..12541)
                     /locus_tag="VYO97_001494"
     CDS             complement(11591..12541)
                     /locus_tag="VYO97_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1754270.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(12554..>12964)
                     /locus_tag="VYO97_001495"
     CDS             complement(12554..>12964)
                     /locus_tag="VYO97_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1754271.1"
                     /translation="GVSDETQISNSFDEEQKESLIKNLVLKNYRENMDVKNSDIIKNS
                     DIIILYGVSLGETDGYIWNQIAEQSIRSSVPVIIYHYVPHFDAGNPTRVKRLYRNVED
                     KFIQNSGIDLELEKKLRDNLIVVIGKTIFNLMER"
BASE COUNT         3895 a         2951 c         2401 g         3717 t
ORIGIN      
        1 ataatcataa tcactacaca aaaaggagat tattatgaaa agaatcattc atgcttggaa
       61 taaggcaagc cttgtcaagc gtatcttgat tggtatgctt tatggggaat cctaggactg
      121 acccttccta atctctcagg gattgggcta ctcggggatt tattcgttgg aggtctaaaa
      181 gctgttgctc ctatcctagt ctttgccctc gttgccaatg ccctttccca acatcaaaag
      241 ggacaagata gcaatatgaa aactgttgtt ttcttgtata tcctatgata gggactttcg
      301 ccgctgctct tgtagctgta ctagcaagtt tcatcgtccc tattgaaatt accctaaata
      361 gtgccaatac tgaaattgca ccaccagatg ggattgggca ggtcctcagc aacctcttgc
      421 tcaaactggt tgacagccca gtcaacgccc tgcttactgc taactatatt ggaatcttat
      481 cttgggcagt catttttgga atcgctatga gagaagccag taaaaatagt aaagaattgc
      541 taaaaactat cgctgacgtg acttctaaaa ttgtcgaatg gatcatcaat ctggctccat
      601 ttggaatcct tggtcttgtt tttaaaacca tttctgacaa gggagtcgga agccttgcca
      661 actacggtat tttattggtt ccattagtaa cgactatgct ttttgttgcc cctgtggtca
      721 accctttgat tgccttcttt tttatgagac gcaatcctta ccctctagtt tggaactgcc
      781 tccgtgtcag cggtgtgaca gcctttttca ctcgtagttc tgcgactaac attcctgtca
      841 acatgaaact ctgccatgac cttggactca acccagatac ctattctgtt tctatcccac
      901 tcggttctac tatcaatatg gctggagtag cgattaccat taaccttttg acccttgctg
      961 cagttaacac tcttggaatt cctgttgact ttgccacagc ctttgtcctc agtgtggtag
     1021 cagctatctc agcctgtggt gcttcaggta ttgccggagg ttccctcctt cttatcccag
     1081 ttgcttgtag ccttttcggt atttctaacg atattgccat acaagttgtt ggggttggtt
     1141 ttgtgattgg tgtcatctaa gactcatgtg aaacagccct taactcttct acagatgtcc
     1201 tctttaccgc cgttgccgaa tacgcagcaa cccgtaaaaa ataactcatc aaggcaagcc
     1261 tgctcatgtc ttgtctttta cgcttttatt ctaacttatt aggaaattct tatgtctatt
     1321 agccaacgta cgaccaagct catcttagct acctgtcttg cctgcctgct tgcttatttt
     1381 ctcaatcttt cgtcagcagt ttcggctgga attatcgctc tcttgagcct atctgatacg
     1441 cgtagaagta ctttaaaact ggctcgcaat cgtctttttt ctatgcttct agctctggct
     1501 atcggtgttc tagcttttca cttgagcgga tttcatatct ggagtctcgg cctctatctg
     1561 gccttctacg ttcctttagc ctacaagatg ggctgggaaa ttggcatcac accaagcact
     1621 gttttggtta gccatctctt ggttcaagag tcaacctctc cagaccttct agtcaatgaa
     1681 ttccttctct ttgctattgg tacaggattt gccttgcttg ttaatctcta tatgccttca
     1741 cgagaagagg aaatccagca ctaccacacg ctggtggaag aaaagttaaa agatatcctc
     1801 cagcgcttca aatactattt atccagagga gacggacgca accgagcaca gctggtagca
     1861 gaattagaca cgcttttgaa agaagccctc agactggtct atttggatca ctctgaccac
     1921 ctctttcacc agaccgacta ccatatccac tactttgaga tgagacagcg acaaagtcgt
     1981 atcctgagaa acatggccca acagattaac acttgtcacc ttgccgccag tgaaagcctg
     2041 atcttagcgc aactcttttc aaaaattgca ggtcaactga gccagaccaa tcctgcttct
     2101 gatttgctag atgaaattga acgttatctg gaagtcttcc ggaaccgcag tctgcccaaa
     2161 acaagagaag agtttgaaac ccgcgccacc cttcttcaac tcctacgtga agccaaaacc
     2221 ttcatccaag taaaagttga tttttaccaa aaatatagac agtaaaaaat aaaaccacta
     2281 aaaaacagag cttgccagca ttcttgaaaa gtagtataat tattgcatgc gcaatcatct
     2341 tgtgacgcag agactgcccg ttaatggact ttcttctata ttatgactct aaaaagaaag
     2401 gagacactat gacctatttg gaaaaatggt ttgacttcaa tcggcgtcag aaagaaattg
     2461 aaagtctctt ggaagagacc attgcccaac agagcgagca aagtctgacc ttgaaagagt
     2521 tctacctgct ctactatctg gacttggctg aagaaaaatc tctacgccag attgacctgc
     2581 cagataaact tcatctgagc ccgagcgcag tttctaggat ggtggctcgc ttggaagcta
     2641 aaaattgcgg tctactcagt cgcatgtgtt gccatcaaga tagacgctct agttttatct
     2701 gcctgacaaa tgatgggcaa aagacactgg cctcgctaca aaagactgtc gaagaaagct
     2761 tggaagcagg tttggatttc ttgatttaag atgattttaa aaaaagttgc gtgcgcaatc
     2821 atttttcttg acattctctt ttataaggag taaaataaag ttagcattaa acaaaggagt
     2881 tttaaatatg attgaaatta cctatataga tgccagcaag aacgaaagaa ctgtgacttt
     2941 cgagtcttat gaagactttg aacgttcaca acaagcttgc cttatcggcg tcgcagacta
     3001 ctaccctgtc caaaaattaa cttacaaggg tcataatttg gactaccatg ggacttatgg
     3061 agatatcttc ttctatctca tgaaacaaga tttaagccaa tataactaaa aaaaggagaa
     3121 atacaatggc aaaagcaatt acagatgcaa cattcgaaca agaaacaaaa gacggtttgg
     3181 tcttagtaga cttctgggca acttggtgtg gtccatgtcg tatgcaaggt ccaatcttgg
     3241 acaaattgtc tgaagaactt tcagaagatg tcttgaaaat cgttaaaatg gacgttgatg
     3301 aaaatccaaa cacagctcgt gcttttggaa tcatgtctat tccaactctt ctcttcaaaa
     3361 aagacggcca agttgtcaaa caagttgcag gtgttcacac agcagaacaa atcaaggcca
     3421 tcattgctga attgagctaa tcaaacgaga gaccaagtac tttatttggt ctcttttttc
     3481 ttgccctttg ccatttttca aaaaatatgc tagactatag tagattgaaa ctagaatagt
     3541 acacctctac ttctaaaaca ttgttaaaaa tcgatttgac tgtcctgatc gatttatcct
     3601 gtttttattt cattttacta tataagtaga atattacgat gtttgggata tgccatcgct
     3661 aaaaaaaact cgatttggta ttttttagtt ccctaatggg agctttttgc gtgctctgaa
     3721 catttcccat tttggaagga gtactgtgaa acgtcaatca gccttggtcg tctttagtgg
     3781 tggtcaagat tcaacaacct gccttttctg ggccatgcaa cactatgaaa cagtcgaagc
     3841 tgtcaccttt gcctacggcc aacatcatca cctcgaaatt caaattgcta gagaaatcgc
     3901 caaggaacag ggcattcgtc accatatcct cgatatgtct ctgctgggac aaatcactgc
     3961 tcaaccagac tttgcgacga ttcatatttc ctacattcct gacaagctct gtgtcgagtc
     4021 aaaatccctc aaactatatc tatttagcta ccgaaatcac ggagatttcc acgaaaactg
     4081 tatcaacacc atcgggaaag acttggtcaa cttgctagac cctcgctatt tagaagtctg
     4141 gggaaaattc actccgcgcg gtggcatttc aatcgacccc tactacaact acggtaagca
     4201 aggaactaag tatgagggct tggcagaaca acgcctcttc caacacgacc tttatccaga
     4261 gaaaattgac aaccgctaaa gtcatactca atgaaaatca aagagcaaac taggaagtta
     4321 gccgcaggtt gctcaaagca gtgtttgagg ttgtcgataa aactgacgaa gtcagcttaa
     4381 aacactgttt tgaggttgca gatagaactg acgaagtcag taaccatacc tacgacaagg
     4441 tgaagctgac gtggtttgaa gagattttcg aagagtatca tacgaaaaag ccctgttcct
     4501 caagatgagg agcaaggctt tttgagtttc aattattctt ctgttccaag gaaatttttt
     4561 gcaacaaggg ctgcaagaac tccaccagcg attggtgcaa ggatgaaaat ccaaacttgt
     4621 tgaagggctg cgccgcctac caagacagct ggtgccaagc tacgagctgg gtttactgaa
     4681 agtccagtaa tcttcaatcc gacaagaatc atcgccatca atgacaaacc gattaccaaa
     4741 ccagcaatcg cgccattgcc cttgctttct gaagtcacag tcatgataac caatacaaac
     4801 aagaaagttg cgatgacttc aaacaagaaa ccaccaaaga cagtgacacc gtttgccaag
     4861 gcattttcac caagactagc agttgacata cctgagttag ccaagaggaa gaagacagcg
     4921 ccagaagcga tgaaagctcc aacaacctga ccaaggatgt agtttacaag ttctgaagat
     4981 gacaaacgtt tgtttacaaa catagcaatc gaaacagccg ggttcaagtg agcacctgaa
     5041 acagttccga ttgagtaggc tgccaccacg attgccaaac caaaggcaaa ggcgattcca
     5101 aggtgaccaa ggccatcaag accatttcca aaaacaacag ctcctgtccc gacgaataca
     5161 agcatgaacg taccgattaa ctcagcaaca aattttttca ttttctttct ccttttttca
     5221 aaaactagat actagtctat caaaagtagg aaagggtttc aagaaaattg attggaaatt
     5281 ttttgaaaat catagaacta ttagctaatc tctagtattg aaaagactgg atagcttctt
     5341 tcaactcatc ttgtaaacta tttctctggt caagttggac atagacttcc accagacagg
     5401 atctaaagtt ggaaaatttg gaaaaatcct ccctttcttc tatcggaaaa tcaacagttt
     5461 ttatccaaga agctacttgt tcttgctcca acttcccttg taaaataggt tcatagatca
     5521 ctcttgctaa acgccaatcc tcatcatctg taaagcgaat cgacattctt ttaaatagtt
     5581 ggccaagtat atcaaatact tcatgaactc tgtttttagg aaagtctgga tgacaaacca
     5641 cctctgtcag taaatcggct ccatgtgcaa aagaatgaac ccaaccatac tgacttgaga
     5701 aacctgttgt atccttttct tttgaaagat agtgcaagcc ttgatttgaa agatcatcac
     5761 gaatttctga ttgtaatccc tgataaaaaa ccgattgctg gttagcatct gcagacaaga
     5821 gatttgcata aacaagtgcc ctaaaagaac gttccagtgt tggcaggcct accttatcaa
     5881 tctctttatc tagtcctaca tcagatgaaa ccccttcagc aatgaaatga aattgctcct
     5941 gtataaatag ctcttcctga atccccttag caaagcttgt aaaaataagg tcatcacgaa
     6001 tttctggaga aggatttccc aaatgatcaa acaaccactg gatttcttcc tggttataat
     6061 ttggtttttc ttctgctatt tttcttagta aatcttgata catggtcaat acctctacat
     6121 ttctagcaac tgttcaaaaa ggcagtctta aatgacctta ccaatttgta aaattaagtt
     6181 aaaaagaaaa agacatttat attcagagaa acagcatatt tctgaaaaga tatataaatg
     6241 atttattcca tattacaaca aataaacttg taatgataga gccttatcac aaaaatcaca
     6301 ttcctgtctc aaaaattgcg aaaaactagt tataataaag tgaaataaaa actatacaaa
     6361 ttgattaggg aatttaaaac aatttctaac aatattttaa aaactgaacc gtactatttc
     6421 aacttccata aattacaata ttgataggca cagttcccat acctgtccaa attcgaataa
     6481 cgtttagtat aaattagaat atgactacaa catcacaaat aattagacta tctataatat
     6541 ctacgatgta acatatacat aggaagcacc gttatcaaga gcgccaaggc tatcaaccag
     6601 ataagatgaa gatacaaacc tgtcacctca cctccccata taatacgaat catgcgagta
     6661 gcatgataca agggagaaaa ccaagtcaaa tcaccaagta ttgggggcaa ggaagtaatg
     6721 ggaaagaagg tatctgaaaa catataaaga ggacggatta ccaaaacatt gtagtaattc
     6781 agaaattccc tagacggcgc caagtaggta aagattagtc ccaaaacacc aaacaaataa
     6841 cccactataa ataaaatcag aggaatcaat aacatcaaga catgaaaatg cgctgtgata
     6901 agcatggaaa tcagccaaaa aatcccaccg aaaatcactg cacgaatacc tgcccagata
     6961 gcctgaccca ccacaatatc actcgtattg attggcgtca tcaacatagc atcataagtc
     7021 ttttcaatct tatactggat aaaggcattg accgttgttt ctgcggtggc cgcagacatg
     7081 gcagtcgcag caattaaacc tgtaatgaca aaatcacggt agagcatacc gttaacatgc
     7141 gtcaaatagg caccaactcc aaatcccatt gctaaaagat aaaagagggg gtcgataagg
     7201 ttggggacaa ttgaaatttt aaataaattt ctaaaactca tgtagttgcg taccacaacg
     7261 tgtccgatgg actgaaatga taacttaaac atgcaagtcc tccttctctg tcccagtcaa
     7321 ccccaaaaaa agcgtctcaa ggttgtcagc atcatacttg atgagcaagt cttctacagt
     7381 tccttcttct ttgatagtcc cttggtggag catcagcacc ctatctgtca aatattcaat
     7441 ctcattcata tagtgggaag taataatgat ggaaacacct aattccttca actccaaaag
     7501 tttatcccaa ataatattgc gggattgaat gtctaaacca actgtcggtt catctaaaat
     7561 gataatatca ggtcgattga tgagagctct tgccaagaca agacgtcgtc gcatcccact
     7621 cgacagctga ttcaccatct tttgcgcatg tttgctcagt tgaacaaaat ccaagagaga
     7681 atcgattctc ttcctaagtt cgacagatga gataccataa catagaccgt gagcaaaaag
     7741 attatcataa acattcaagg tcatgtccag catatcctcc tgccctacaa tacccagaca
     7801 agatttactc ttacgacgat tggtttcaat attttgcccc aagatatcaa tctctcctga
     7861 ggaaattgag gtcagtgagt aaatcatctt taaagtggat gttttgccag caccgttgat
     7921 tcccaagata cccaaacact ctccttatcc caattcaaag ctaattccct tgacagcttc
     7981 ataagaacca tattgctttt ttagattttt aacacttaac ttcttcactc tttctttccc
     8041 ccacaaatag agcatgttgg atactttgtt aattcatctc acttttgaca aaatgttgag
     8101 aatgtaatct taagattaac gtccttcaac tgtggatact gctgcaatta cttttgtaat
     8161 tttacctcca tttaaactca gacaataaaa tttaaaatat tctctagtaa aacattttta
     8221 aaatctattt ttcttgtgct ttaattatag tataataaaa gatagaaaat catcgatata
     8281 tgaaatttgg agaaaatttt tatggaacca gatattttca tcatcacaaa actcttatca
     8341 gacccttctc ggatgcacat tttagatatt ctaatggatg gaaaagctca cacggttaac
     8401 gaaattgcca gctttaccaa aatcaagcaa catactgtca gctatcacct caaattactt
     8461 accgaagcgc aggtaaccac tctacaaacc tatggacgtt ttcattatta ttctttaaaa
     8521 agtgctgcca ttgcagaaat gctggaattt ctcagcttct actctcctca gcgtgatgtc
     8581 aaaagttata aacaacacat tcacaaaaag gaattaaagg tcgctcgcac ctgctatgac
     8641 catattgctg gtgagcttgg catttccatt accaattatc tcttgcaaga aaatctcctc
     8701 agcgaatcag aaaaagactt ccaactcact gaagctggga agtcttattt ccaaagaaaa
     8761 ttacacattg atacggatga attgaaaaag aaaaaacgga aattttgccc taaatgtttg
     8821 gattggagcg aacgtaaaaa tcatgtcggc ggagctctcg gcaatgctct tttagaattt
     8881 ttcagtgaaa aacaattggt catccctgca caaacgccac gttcacttac cattacagag
     8941 aagggcaaag aattcctcga aaaggaatgg gggatttaaa catggacttt taagcaaata
     9001 actgtccata cccattctat aacaacctct ctatcccctt actcccccaa ctcagcactg
     9061 taggcgataa tctggtcaac tgtgtcagac aagaattgga tggtatcacg gagtggtttg
     9121 tctgttgaaa tatcagcacc gataatcatg gctgactcaa gtggtgtctt gctaccacct
     9181 gatttgagga gattgagcca gtcttcagct ccagtttctg agtttttaag atgaaggtaa
     9241 ccagctgtcg agatgacaag accagcagag taagtgtaac tatacaagcc catatagtag
     9301 tgagcttggc gcatccaagt caaagctgca tcgtcgtcga tttcaatggc atcgccccaa
     9361 aaatctgtca aaacttcctt cataatgctg ttgagtttgc ttgctccgaa ggtttctcct
     9421 tcttcaatca atgtatacac cttacgctgg aaggcggctt ccaagaggtg ggtgataaag
     9481 ttatggaagt aggtgtctgt caagcgatga gccagagcga agcgtttttg acgtgggtca
     9541 ttagattggt tctctaagta atcactgagt agcaattcat tgaaggtcga cggtgcttca
     9601 acataatagg tcgacatgtg ggcgttgaaa tagctttggt gattgtctga aaagatgaat
     9661 tgaccagaat gcccgatttc atgaatcaag gtatagacat cgctcaaacg gcctgtccag
     9721 ctcatgagta cataagggtg tacgcgatat gggtccgccg cataaccacc ggaatccttg
     9781 ccactgttag cagcaaagtc cacccagcgc tcttcttgat aacgagcaac ttcctgacaa
     9841 tattcttgcc ccaaaggttc taccgacttc atgaccaaat cataggcatc gtcaatagtc
     9901 acttcaggat tgagggcgct gtccaagtcc aatttccagt ctgcaaaggt catcttttca
     9961 agaccattta ccttggcaac atgcttgagg tatctctgag cgactggtgc aaagtccttc
    10021 atgatgaggt caatctggcg gtcaaacatg acacggtcca cttcttgttc agctagaaga
    10081 tagtcaaaga cagagtcgta tcccttcata tcagccaaga gtttttcaga cttgacctga
    10141 gccagatagg ctgctgcagc cgtattttgg tgcttacgaa gtccctctga gaaggaacgg
    10201 aaggatttct cacgaacctc agcatcctcg tgattttggt agaaattctc ataggtgaca
    10261 aagctgtttt tataggtctt gccatgggct tcaaagtcag ccatttcaaa atccccagct
    10321 cgcatcttag tataaatgtc ctgcggacta tagaaaactt caccgagatt ggtcaaggcc
    10381 ttctccacat cagcccctag atagtgggct tttttgattt tggcctgacg aatagctgcc
    10441 gtcaagtgag gcaattcacc caaacggtcc aagacttcct cgtctgctac caccaaggca
    10501 tcgtcaaaga aggtcaaggc tacgctggca tctgtttcaa attccatccc agcttgggca
    10561 atattggcaa attcgtcatt gctatagtcc gtcgtctgag gcataaaagc atagttgcca
    10621 atatggctca tctgaatgta gatctgttcc aattccgcaa aggccttctc gaaatcctca
    10681 aaagtgtgaa gattgccctt gtaatcacgg ctaaactggt tgatgtcttc acgagctttc
    10741 tcgattgcac gcaagaaatc ctcacggtct tggtataggg ctgttaaatc ccagagttcc
    10801 ttctctggaa attctgaacg gtgtttttgt tccattttct tcctcttatt tctctaattc
    10861 tactaaaaca ctaagggctg ataaggcgta aagcggtgct gtttctgctc gcaaaatacg
    10921 aggacctagg cctgccaaaa ctgctccttt agcttcaaaa ctttcgattt ctgcaggtga
    10981 cagaccacct tctggaccaa agataaagag cagtttggct cctgtttcaa gaccagtgac
    11041 tgcttgcaga agcgcagcgg cttctccttc tttagctgat tcttcatagg ctactatgat
    11101 agagtcaaac tggtccagct gagctagaaa atctgctttt ttctcgaaaa gtttaatact
    11161 tggtacaata ttacgcttgc tttgctcggc tgctccaagg gcaatttttt ctagtttttc
    11221 aactttttta cccaatttct tgccatccca cttggcaact gaccagtctg caggaaaggc
    11281 ccagatttgg ctagcaccca gttcggttac tttttgagcg atgaactcca gcttgtctcc
    11341 cttgggaaat ccagatgcga tggtcacttg gactggtagt tccacattgt catttaattc
    11401 ttggaccaac tcaaactgac gattttccat atccagcacg cgcgccaagc gcttgatgcc
    11461 atcatcaaag actaaggtaa cctcatcctc ttctttcaag cgcataacct gaaacatatg
    11521 cttactggtt tccttgtcct cgatagtgac aggagagata gcactgcctt ttacaaaata
    11581 ctgctgcatg ctagcctcca atcacaccag agatatcctt ggttttctta aagacacagg
    11641 tattccattc cccttgaacc atgtgagttt cgaggaaaaa tccagctgac tcagccgact
    11701 ggcgcaccat gtcccacttg tccttgataa tgccactcat gatcaggtag ccttcatcct
    11761 ttaccaagcg ataagcatcg tccgtcagat gaatgagaat atccgccaag atattagcca
    11821 cgatgacatc tgcctcaatt tccacaccct taagcaaatc tccagccgct acatggatat
    11881 tttccatgcc agggttgagc tcaatatttt cctgagccac acgaaccgcc acatcatcca
    11941 ggtcataggc gaaaatttct ttagccccca gaagcgagct ggcaatagag agaacccctg
    12001 aaccagtccc cacatctagc accgtttcgc caccacgaag aacctgttcc aaggcaaaaa
    12061 ggctcatctt ggtagttggg tgggttccag taccaaaagc catgccagga tccagcttga
    12121 taattttttc tcccgcagtc gcctcatagt ctgtccaaga gggaacgatg gtcaaatcat
    12181 gagtgatacg agctggctca tagtatttct tccagttatc tgcccagtct tcctcagcca
    12241 aggcagtcgt acctattttt aactctccca aatccataaa atccgtcaat tctgatatgc
    12301 gagcctgcaa gtccgcctca accactgtca catccaccgt gtcagggtag taggctgtca
    12361 ctacgatttc ttcttgctgc tcgacctctg ggaaaatctc gccgaagcgg tcaacatttc
    12421 ccacatagtc cagactgtct tcaatcgcaa caccttgcgc tcccagctca atcaagagat
    12481 tggaaaccaa ctcctctccc tcacgcttca ctgtaacttt taactcttgc catgtttcca
    12541 ttatgtattt tgcttacctt tccatcagat taaaaatagt ctttccaata acaacaataa
    12601 ggttatctct taatttcttc tctaactcta aatcaattcc actattttgt atgaatttgt
    12661 cttctacatt tctatataag cgttttactc tagtggggtt tcctgcatca aaatgaggta
    12721 cataatggta aattatgaca ggaacagaac ttctgattga ttgttcagca atctgattcc
    12781 agatataccc atcagtctct cccaaagaaa ccccataaag aattatgatg tcagaatttt
    12841 ttataatatc gctattttta acatccatat tttctctata gttcttcaaa actaaatttt
    12901 ttattaagga ttctttttgt tcttcatcaa aactatttga aatctgtgtc tcgtcactaa
    12961 ctcc
//
LOCUS       DAVMAF010000052        12905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMAF010000052 DAVMAF010000000
VERSION     DAVMAF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.052"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(98..610)
                     /locus_tag="VYO97_001496"
     CDS             complement(98..610)
                     /locus_tag="VYO97_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1754272.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1209)
                     /locus_tag="VYO97_001497"
                     /pseudo
     CDS             complement(635..1209)
                     /locus_tag="VYO97_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(1222..1626)
                     /locus_tag="VYO97_001498"
                     /pseudo
     CDS             complement(1222..1626)
                     /locus_tag="VYO97_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1677..1763)
                     /locus_tag="VYO97_001499"
     CDS             complement(1677..1763)
                     /locus_tag="VYO97_001499"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1754273.1"
                     /translation="MKIGQRIMRFGIKKLNIGVVSVAVGFDF"
     gene            complement(2070..2879)
                     /locus_tag="VYO97_001500"
     CDS             complement(2070..2879)
                     /locus_tag="VYO97_001500"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1754274.1"
                     /translation="MTFLNKIHETATFLKEKGIQTPEFGLILGSGLGELAEEIENPVV
                     VDYADIPNWGRSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGYGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSKAYTP
                     EYRATAHQVAKKLGIKLDEGVYIGVTGPTYETPAEIRAYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2876..3436)
                     /locus_tag="VYO97_001501"
     CDS             complement(2876..3436)
                     /locus_tag="VYO97_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1754275.1"
                     /translation="MTCYALLVRGINVGGKNKVVMAELRQELTELGIEKVESYINSGN
                     VFFTSTVPKARLIEKLETFFAVHYPFIQSFSLLSQEDYEAEVENLPAWWNEDLARKDV
                     LFYTELLDVEQVITTVESLELKDEVVHFGKLGIFWGKFSEESYPKTAYHKYLLKMPFY
                     CHITIRNAKTFDKIGQMLKNNKGDTQ"
     gene            complement(3438..4649)
                     /locus_tag="VYO97_001502"
     CDS             complement(3438..4649)
                     /locus_tag="VYO97_001502"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1754276.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVPAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFAPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGVIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4663..5346)
                     /gene="rpiA"
                     /locus_tag="VYO97_001503"
     CDS             complement(4663..5346)
                     /gene="rpiA"
                     /locus_tag="VYO97_001503"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1754277.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHIVGVVEHGLFNQMVDKVIVAGRDGV
                     QILTSKKGK"
     gene            complement(5644..6483)
                     /locus_tag="VYO97_001504"
     CDS             complement(5644..6483)
                     /locus_tag="VYO97_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1754278.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6497..7318)
                     /locus_tag="VYO97_001505"
     CDS             complement(6497..7318)
                     /locus_tag="VYO97_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV1754279.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7455..8312)
                     /locus_tag="VYO97_001506"
     CDS             complement(7455..8312)
                     /locus_tag="VYO97_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1754280.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8466..9200)
                     /locus_tag="VYO97_001507"
     CDS             complement(8466..9200)
                     /locus_tag="VYO97_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1754281.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9200..9883)
                     /locus_tag="VYO97_001508"
     CDS             complement(9200..9883)
                     /locus_tag="VYO97_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1754282.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            10073..10303
                     /locus_tag="VYO97_001509"
     CDS             10073..10303
                     /locus_tag="VYO97_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1754283.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            10532..12790
                     /locus_tag="VYO97_001510"
     CDS             10532..12790
                     /locus_tag="VYO97_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1754284.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLKEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KKKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         3845 a         2900 c         2348 g         3812 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattgtac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagctca atccgaacat agcgcacttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatgttatac gtttttactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaaactgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagatcg aggcagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt ccactaacag ctaatttacg
     1201 actttccaaa ccaacatccc agaaaccatt cttagcattc caaacagttc tggaagtaat
     1261 gactttagga gcactgaaag ttgtcacttt cctcaataca tggttataaa aatcaggata
     1321 tttttggtaa gcttcaacct ctgcaatctg agctaggtag catccaagag aatgacctgt
     1381 catatacaac ttagtaatac ttatatcctt ggctaattcc ccaacaacct tgcggatatc
     1441 atccgcttga gctggctctg ttatctctac ttttgactct tcccttttgt tttcaaggac
     1501 ttcacttgct tccagaagcg tttgaggagc tagtcttggc tcctctctaa gaggagtttc
     1561 tagttttgga gcatcatttt ctgactcttt caattcctcc ttagaatcta gtacacctat
     1621 taccacttca gatgttacat cttgctttac ttcattggct gaaattccag ctggagctag
     1681 aaatcaaagc cgactgcaac agatacaacc ccgatattta atttttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattattctt tatctttctt ttcgtttatt tgtaagcaga
     1801 ctcaagcgaa atctccttgt cccctatata ggacaaggct aagaaattca gaaagtaaat
     1861 tcattcttat ctttccttat catcctagtc tgcttggtag gaagctaatg ctagtatcct
     1921 agaatcttgt ctttcacaat gctaactatc agactctttc agttacaaga gtaggaagaa
     1981 acccatagtc tacctatgtt tcttatttgt tccgaatagc cagtcaatca tgaaaaggta
     2041 gaggtattcc cccttttaaa tcttttttct tacaattcag caagaatcgc tttaagcaag
     2101 cccttgaagt cacctttgac acgttcggtt acttccacaa cttcttcgtg gttgagttct
     2161 tcttggaaac cagccgcaaa gttagtaata catgaaattc ccagaacttt caagccagag
     2221 tgggctgcca cgatcacttc aggaaccgta gacataccaa ctgcatctgc tcccagtgtc
     2281 ttataggcac gaatctctgc aggtgtttca taagtcggac cagttacacc gatatagaca
     2341 ccttcatcaa gcttgatacc aagtttctta gccacttgat gggcagtagc acggtattct
     2401 ggtgtgtagg ctttagacat atcagggaaa cgtggaccaa agtcatccaa gttttcaccc
     2461 atcaatggat tttgccccgt catattgata tgatctgaaa tagccatcaa agtaccaggg
     2521 ccataaccaa taccaccagc tgcattggtt acaatgacac cttcacatcc aagaactttc
     2581 atgacacgta ctgggaaagt cacaacttcc agagggttcc cttcgtagaa atggaaacgc
     2641 ccttgaagag ctaagacctt gcgacctgca aggtcaccat ataccaattt accagcgtga
     2701 ccaactactg ttgaacggcc ccagtttgga atatccgcat agtctactac aactggattt
     2761 tcgatttctt ctgccagttc tccaagtcct gatccaagga ttagaccgaa ctcaggcgtt
     2821 tggattccct tttctttcag gaaagtagca gtttcatgga ttttgtttaa aaatgtcatt
     2881 gtgtgtctcc tttattattt tttagcattt gaccgatttt gtcaaaggtt tttgcattgc
     2941 gaatggtaat gtggcaatag aaaggcatct tgagcaagta cttgtgatag gcagtcttag
     3001 ggtaggattc ttcagagaat ttcccccaga aaataccaag ttttccaaaa tgaacgacct
     3061 catctttcag ctctaaactc tcaactgtcg tgatgacttg ctccacatcc aaaagctcag
     3121 tgtaaaagag aacgtctttt cgtgccaagt cttcgttcca ccaagctggt agattctcca
     3181 cttctgcttc atagtcttct tgactcagta gggaaaaact ctgaataaat ggataatgga
     3241 ctgcaaagaa agtctctaat ttttcaatca atcgggcttt gggaactgtc gaagtaaaga
     3301 aaacattgcc actgttgatg tagctttcaa ccttttcaat tcccaactct gtcaattctt
     3361 gccgaagctc agccatgacg accttattct taccaccaac attgatgcct ctcaccagca
     3421 aagcatagca tgtcatctta taccaattta tctaagaaac tttccccaat catggcagtt
     3481 tccacaccaa agttatcggc aacagtcgct gaaatatctg caaaatgtcc tactggaatg
     3541 acaccatttc ctttaaaagc aggactgtaa gccaaaagtg ggatatattc gcgagtgtgg
     3601 tctgttcctg cgtaagttgg gtcgttcccg tggtccgcag taatcaagag aaggtcgttc
     3661 tctcgcatgg ctgcgataat ttcaggtaag cgttcatcaa actcatgcaa gcaatcacgg
     3721 taaccgtgag cattacgacg gtggccgtaa agggcatcaa agtcaaccag gtttgtgaat
     3781 gagaatcctt tttcaaactc agcaagtccc atagtcttca atagtgtatc aattccatgg
     3841 ctgtttgact tgttatggcc catgtcgtgg ttgataccgg caccgttaaa gatatcgttg
     3901 attttaccaa cagcataagt atcgatacca gcctcattca atttatccaa aacagttggg
     3961 gcaaatggag atacagccaa gtcacgacgg tttgccgtac gagtgaagtt acctggctca
     4021 ccaacatatg gacgggcaat gatgcgacca agaagggcag gacgctcaag ggtaatcgaa
     4081 cgagcgtatt cacagatacg gtacaattca tccagaggaa tgatgtcttc gtgagcagca
     4141 atttgcaaaa cagggtcagc tgaagtatag ataatcaact ctccagtttc catctgacgt
     4201 ggtccaaaat catcgataac ggctgttcct gagtaaggtt tgttagcttc acgaatgacc
     4261 ttacgtcctg aaaattcttc gatttttgtc aggatttctt ctgggaatcc gttccagaaa
     4321 gtatcgaaag gctccgtaat gttgagtccc atgatttccc agtgcccagt catggtgtcc
     4381 ttaccaaggg acacttcttc caattttgtc gcatatccag ttggattgct ttctgccggt
     4441 acagtcttaa gaggagtttc acgaggaata tttccaagac cgattttagc catgtttggg
     4501 acattcaaac caactgtttt tgaaatgtgt cccagtgtgt cagaggcacc atctggaacc
     4561 cctgcattga caaagttatt agcatctggt gctgcaccga ttcctacaga atccagtacc
     4621 accaaatgaa tacgattaaa tttagacatc ttatgccccc ttctattttc ctttttttga
     4681 agttaaaatc tgaactccat ctcgtccagc aacgattacc ttatccacca tttggttgaa
     4741 taaaccgtgc tccacaacac caacgatatg gtccaattct tgtccaaaag caattggatt
     4801 ttcaatgaca tccaaggcga ggtcaatgat aaaattctgc atatccgtca caaaacgttg
     4861 gccatctttt tcacggaaac ttggtttgta gccagctcgt tcaaaacgac gaaagacctg
     4921 ttctgcacca tactgaacca cttctactgg caatttaaaa gcacctagtt tttcgaccag
     4981 cttgctttca tccaccaccc aaatgtattc ttttgatggt gtcgcaacaa ccttctccat
     5041 cagaagagca ccaccaccac ctttgattcc attaaactga ctatccactt catctgcgcc
     5101 atcaactgtc acatcgacaa agtctacttg gtcaatagat ttgagtggga tattaaggcc
     5161 ttctgcctgt ttagtggtca cactagaagt cgttacagct ataatctgca atccttcttc
     5221 cttgatacga cgaccgattt cttcgacaaa ataataggca gtcgagcctg ttccaagtcc
     5281 aacgaccatt ccatccttga caaactcagc agccttgata cctgccattt tcttcagatt
     5341 ttccactcaa acacctccat taaagagcta cctctattat accatgtaaa ggcttttatt
     5401 tcatctaaaa gtacctttag aatactgctt atttatcctt gaaattaaat ttttttaata
     5461 aaagcagaac tctgacaaat cactttaagt acggatgtaa accaaatcaa ctctaacaaa
     5521 aatagattta caagagtaaa aaaacgaaca aatttagttt cttagcgaga agaatactaa
     5581 acgttcgttt tttgtgaatt aagaataatg atacaaatta tcaatatttt ttgtctaact
     5641 attttagaca tatcttttca ttactttagg aagacaatta ctaatctccg ttggcaaaac
     5701 cacatactgt tcttgagcta gttcttgggc tatggctgaa tgaagatggg tcgctactgc
     5761 cacacgttcg taaagactag cttgcttaaa ctggcctgca aatcctgcaa tcatcccagc
     5821 aagcgtatct cccattcccc cagtcgcctg atagggaccg ccaacctgta actggtaata
     5881 atctgattgg ccagcttgcc aaatacgagt agctgaacct ttctccacca aaattgttcc
     5941 ttgagggaag gaagttaggg cactagccgt tgcatcttcc ttttgctttt caatcgtaat
     6001 tccagataat ttttcccatt ctttttggtg aggagttagg ataagctggc ttgaggaaaa
     6061 tgacaaactt gtcctagcaa gaatggtcaa ggcacctccg tctacaatca aaatctgatt
     6121 ttgtcttaag ccagcaaaga cctgtttaac tagatcttct ccaaaagcat cgtctcgtaa
     6181 acccggcccc agcaagacaa cttctgcctt ctccaattgc tcttttaaca actgctggtc
     6241 ttgaagagaa aaggccatag cctcaggtaa atggctgtgc agggctggaa tattttccct
     6301 atctgttcca acggtcacca atcctgcacc gctttttaca gctgccaaag cagccatgat
     6361 gatggcacca ccataaggat aagttccacc aagaaacagc agacggccat agtctccttt
     6421 atgactagta cgagaacgtt caataataac tttttctagt aaggcttgat caatcacttt
     6481 catcgttctt cctcaactat cctttatctg attgttttaa aacaacttga tagactttct
     6541 tagtccactc ttctaagtct tctccagcat agtcaagata atgcacccta tcttgtattt
     6601 tagttggaat aatattttca aattggctct tattatgagt tggaataata caaatataca
     6661 actcactaaa taaattcaac atgtccttaa tttttgcaac atggtagcca tcaaaaatat
     6721 aaccagtaac tttaatcaaa tctgtataga taaaatgaaa actcactcca ggaataaact
     6781 tgtcttgtaa ttctttctct gaaggagctc gacctaataa acgctccatc gctaatcgat
     6841 aaccagaact tgtatttgac caccctaaca tgacataatc aaaataatct atcggatccg
     6901 atgcagcatt tctactatcc aaaaccaact cttcagcatc tcgcctaaag gctttcacag
     6961 cagataagag ctgacctgtt ttaaagatag aaatcgctgc atctatagtc gtagtatgac
     7021 tacagtagtc tgaagtcact aacctcttat gtagatcata agtcgaggct actcttttat
     7081 ctgtaggggt aaatggtggc agaaagggat gctgacagat gtaatctaaa atttcttttt
     7141 ttaaacaaga atcagcacac tcaatacttg tttgacgttt atgaagtttt tcgtaagcta
     7201 taaatttagc aattttttct aattcccact cttgaatatt tggataatca attagtgcta
     7261 ggtagtatat tccatcccat tgtaaactat attccctatt ataaattttt aaaatcatct
     7321 aatcacctca ctccattata caacaaaaag gaggcgcaga cctccttttg taatcttata
     7381 tctaaaattt aatattcatt tctgccattt taaatatagc tatagaaaat acactctctt
     7441 cagcgatttt tctcttattt tctatccaat gtccgaagtg ctgcttgata agtttgctcc
     7501 atcagcatag taatggtcat agggccgaca cctccaggga ctggcgtaat atagctagca
     7561 agtggggcaa ctgcctcata atccacatcc ccacagagct taccattttc atcacgattc
     7621 atcccaacgt caatgactac cgcacctggt ttgacaaagt cagcagtcac aaacttggca
     7681 cgaccgattg cgaccaccag aatatctgct ttagcagcca ccttagcaag attatgggtg
     7741 cgtgagtggg tcaaagtcac tgttgcattc ttggccaaaa gaagctgagc catgggtttt
     7801 ccaacgatat tagaacgacc gatgacaacc gcatttttac cttctaattc aatcccatat
     7861 tcatgaaaca tttccataat tcctgcaggt gttgatggaa tcatgactgg atgtccagac
     7921 caaaggcgtc ccatatttag aggatggaaa ccatccacat ccttttctgg gtcaatagcc
     7981 aatagaaccg cctcttcatc aatgtgtttt ggtaatggca actgaaccaa aatcccatgc
     8041 caagctggat cctgattgta tttagcaatc aggtctaaca attcctcttg agtaatggtc
     8101 tctggaactc gcactacttc gctacgggaa ccagccgcaa gagctgacct ctccttgttg
     8161 cgaacgtaga cttggctggc tggattatcc ccaaccaaaa tcactaccaa accaggcact
     8221 agagctgttt cttcctttaa ttttgcagtc ttttcagcca actgcccctg caatttggcc
     8281 gctaaagctt tcccatcaat aatctgtgtc atatcacttc tcttttcttt caaatatctt
     8341 ccattatacc aaaaactgct agtgccctct aacacttctt tcctaggtta tcctatagtt
     8401 tcaaactata agaaaaagct ctggttgaac ttttcactaa tcttgtcttg aaattttaaa
     8461 atagattata aaaccttact caagaaatct ttagtccgtt gttcttgggt ttgttcaaaa
     8521 atctgctcag gtgtcccatc ttcaacaacc acaccgtctg ccataaagat aacacgatct
     8581 gccacctcac gggcaaatcc catctcatgt gttacgataa ccatagtcat ccctgacttg
     8641 gcaaggtctt gcataacagc caatacctca cctaccattt caggatccaa ggctgaagtt
     8701 ggttcgtcaa agagcaaaac atctggttcc atagccaacc cacgcgcgat ggcaatccgt
     8761 tgttgttgcc cacccgacag actctgtgga taagcgtctg ccttatctgg caaaccaact
     8821 ttttccaaaa gctcttgggc tctcttctca gcaacttccc tgctttcacc tttagtcttg
     8881 ataggtgaca aggtaatatt ttccatcaca gtcatattag gaaagaggtt aaattgttgg
     8941 aaaaccatgc ccatcttctc acgcatggca aacaggtcat tcttcttgtc cgtaatatcg
     9001 actccctcaa agataagctt ccccttggtt gcttcctcca acaaattcat agagcgaagc
     9061 aaggtagatt tcccactccc tgaaggaccg atgataacga caacttctcc tcttttaatc
     9121 tcgaggttga tgcccttcaa tacttcattc tttccaaaag atttatgtaa attttcaatt
     9181 tttatcaagg tttctgtcat tatttcttat ctccttgtcc catatgtttt tcaaaagctt
     9241 tcaaggctac tgtcagaata gaggtcataa tcaagtagta aaatgctgca aataaaagtg
     9301 gtgttaaagg tagataggtt gttgtagaaa ctgttgtagc cccattccac aactccatga
     9361 ccccaatagc tgataagagg gagctgtcct tgataatggt gataaattcg ttccccaatg
     9421 ctggcaagat atttttgact gcttgtggca aaatcacata acgcatcgca tttttaggac
     9481 gaatccctag cgaataagcc gcctctagct gaccttttgg aaccgcattg attccggcac
     9541 gaacagtctc cgaaacataa gcaccactat tcatagagat aatcaaaatc cctggaatca
     9601 gacgcgaaaa atcaacacct aaaattccaa tctgaatagt cggagcattg atatgcataa
     9661 gagcaaaggc aatcataatt tgaaccatca tcggtgtccc acggaaaatc caaacgtaca
     9721 agttggccaa ccaaacaagc ggtttaaact ttgaacgttg cccaaaagcc aagacaacac
     9781 ccaaaatagt tcccaaaaag ataacacaga tagaaataag aatcgtcaca acagccccat
     9841 agttaaaata aggtaaatac ttaggtaaaa aagaaaaatt catagtcaca ctgctttcta
     9901 aaatagaata ctgtatgatt ataacatgtt ttgaattaaa atttaaattt tttatccaat
     9961 ttatttaaaa acctttaaac tagcagaatt agcgagaaat cttagttttt tctagtcaag
    10021 ttggcctcct ttatggtaaa atagtaacga taagaaatga aggagaatca aaatggaatc
    10081 acatttggtt agaatcatca accgccttga agcaatggca aacgatggcg gaaatttaaa
    10141 acgtaatttt gaacgcgaag gagttgttgt tgcagaagtt gcgtacagcc acgacgaaga
    10201 aaacggccca atctttaccc ttcgtgacgt agaagatcgc gaaacgtata cgtttgatag
    10261 cattgactta atcgcaatgg aaatttacga acttctctac taatctaaaa caagctggga
    10321 ttgtccctgc atgtctaagt aaaatccttt ttgtctcaca aataggattt ttttatagtc
    10381 caaattcaca aaaattttca ttgtaacaac ttctcaaagc ttcaatcttt ttacttgctt
    10441 tacaccccaa tcctgttata atgagagtat agaatttgac tatttttgac ctttaaacaa
    10501 gtcaggctaa agaaagaaat gaggtagatg tatgctttgt caaaactgta aaattaacga
    10561 ctcaacaatt catctttaca ccaatctcaa tggaaaacaa aaacaaattg acctctgtca
    10621 aaactgctat aagattatca aaacagatcc taacaatagc ctcttcaaag gtatgacgga
    10681 tctgaacaat cgtgacttcg atccctttgg tgatttcttc aatgatctaa acaatttcag
    10741 accttctagc aatactcctc ctattccccc aacccaatca ggtggaggtt acggtggaaa
    10801 cggcggttat ggttcccaaa atcgtggatc tgctcaaact ccgccaccta gccaagaaaa
    10861 aggcctgctg aaagaatttg gtattaatgt aactgaaatt gcccgtcgtg gagacattga
    10921 ccccgttatt gggcgcgacg atgagattat ccgtgtcatc gagattctca atcgtagaac
    10981 caagaataat cctgtcctta tcggtgaacc tggtgtcgga aaaacggccg ttgtcgaagg
    11041 tctagctcag aaaattgtcg atggcgatgt gccacataaa ctccaaggta aacaagtcat
    11101 ccgtctggat gtggttagct tagttcaagg aacggggatt cgaggacaat ttgaagaacg
    11161 catgcaaaaa ctcatggaag aaattcgcaa acgtgaagac atcatcctct ttatcgatga
    11221 aatccatgaa attgttggtg ctggttctgc gagtgatggt aatatggacg caggaaatat
    11281 cctcaagcca gcccttgctc gtggagaact gcaactagtc ggtgctacta ccctcaatga
    11341 ataccgtatc attgaaaagg atgctgccct cgagcgtcgt atgcagcctg ttaaagtcga
    11401 tgaaccaacg gtggacgaaa caatcactat tctcaaaggg attcaaaaga aatacgaaga
    11461 ttaccaccac gttcaatata cagatgctgc gattgaagca gctgcaactc tttccaatcg
    11521 ctacatccaa gatcgcttct tgcctgacaa ggccattgac ctcctagatg aagctggttc
    11581 taagatgaac ttgaccttga attttgtgga tcctaaagta attgatcagc gcttgattga
    11641 ggctgaaaat ctcaagtctc aagctacacg agaagaagat tttgagaagg cggcctactt
    11701 ccgcgaccag attgccaagt ataaggaaat gcaaaagaaa aagatcacag accaggatac
    11761 tcctatcatc agcgagaaaa ctattgagca cattatcgag cagaaaacca atatccctgt
    11821 tggtgatttg aaagagaaag aacaatctca actcatccat ctagccgaag atctcaagtc
    11881 tcatgttatt ggccaagatg atgcagtcga taagattgcc aaggctattc gccgtaatcg
    11941 tgtcggactt ggtaccccta accgcccaat cggaagcttc ctcttcgttg ggccaactgg
    12001 tgtcggtaag acagaacttt ccaaacaact ggctatcgaa ctttttggtt ctgctgatag
    12061 tatgattcgc tttgatatga gtgaatacat ggaaaaacat agtgtggcta agttggtcgg
    12121 cgcccctcca ggttatgttg gctatgatga ggctggtcaa ttaactgaaa aagttcgcca
    12181 caatccatat tctctcatcc ttctcgatga agtggaaaaa gctcacccag atgttatgca
    12241 catgtttctt caagtcttgg acgatggtcg tttgacagac gggcaaggac gcaccgttag
    12301 cttcaaggat gccatcatta tcatgacctc aaatgcaggt acaggaaaga ccgaagctag
    12361 cgttggattt ggtgctgcta gagaaggacg taccaattct gtcctcggtg aactcggtaa
    12421 cttctttagc ccagagttta tgaaccgttt tgatggcatt atcgaattta aggctctcag
    12481 caaggataac ctccttcaga ttgtcgagct catgctagca gatgttaaca agcgcctctc
    12541 tagcaacaac attcgtttgg atgtaactga taaggtcaag aaaaagttgg ttgacctagg
    12601 ttatgatcca aaaatgggag cacgcccact tcgtcggact attcaagact atattgagga
    12661 cacaatcact gactactacc ttgaaaatcc aagcgaaaaa gatctcaaag cagttatgac
    12721 tagcaaggga aacattcaga ttaaatctgc caaaaaagct gaagttaaaa gttctgaaaa
    12781 agaaaaataa atcctataga aaaggagtag aaaatgaaat ttttctgctt ctttttttac
    12841 taaaataact gtaatttctt gacagcttgc cctttgtcca ttatgatata tagtagactg
    12901 aatct
//
LOCUS       DAVMAF010000053        12730 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMAF010000053 DAVMAF010000000
VERSION     DAVMAF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.053"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            628..840
                     /locus_tag="VYO97_001511"
     CDS             628..840
                     /locus_tag="VYO97_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754285.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            924..1727
                     /locus_tag="VYO97_001512"
     CDS             924..1727
                     /locus_tag="VYO97_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1754286.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1784..3325)
                     /locus_tag="VYO97_001513"
     CDS             complement(1784..3325)
                     /locus_tag="VYO97_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1754287.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3327..3809)
                     /locus_tag="VYO97_001514"
     CDS             complement(3327..3809)
                     /locus_tag="VYO97_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1754288.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GLLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3809..4894)
                     /gene="rlmN"
                     /locus_tag="VYO97_001515"
     CDS             complement(3809..4894)
                     /gene="rlmN"
                     /locus_tag="VYO97_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1754289.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKLNRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4915..5445)
                     /locus_tag="VYO97_001516"
     CDS             complement(4915..5445)
                     /locus_tag="VYO97_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1754290.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(5602..6207)
                     /locus_tag="VYO97_001517"
     CDS             complement(5602..6207)
                     /locus_tag="VYO97_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1754291.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6348..7385)
                     /locus_tag="VYO97_001518"
     CDS             complement(6348..7385)
                     /locus_tag="VYO97_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1754292.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7419..8354)
                     /locus_tag="VYO97_001519"
     CDS             complement(7419..8354)
                     /locus_tag="VYO97_001519"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1754293.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8907..10097)
                     /locus_tag="VYO97_001520"
     CDS             complement(8907..10097)
                     /locus_tag="VYO97_001520"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1754294.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10474..11817)
                     /locus_tag="VYO97_001521"
     CDS             complement(10474..11817)
                     /locus_tag="VYO97_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1754295.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(12197..12709)
                     /locus_tag="VYO97_001522"
     CDS             complement(12197..12709)
                     /locus_tag="VYO97_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1754296.1"
                     /translation="MPAEVLSLLPQSGTYTTSSSVAIAFVPEAPMPVEVAVANGTWTF
                     LGYQEVEEGSNLTFVGKWVFEAKQDPSPQPQPAPTPQPAPQPNPVPPVNPGGEQGTDN
                     NQADRHQSEQPRKPEENPPAQGPAGEKEKQATLPNAGSTAPLSLTGLVTSSLLAGLAV
                     LLLGRKRKDD"
BASE COUNT         3751 a         2775 c         2388 g         3816 t
ORIGIN      
        1 tatcgcctga acgagagttg gatacttggc ttctaagtcc caagcccgtt cccaagcgaa
       61 atatggactt attagtagat gattcactag caggagatat cattttacta tggagaatcc
      121 agtttggtac ttttaccact gaaacatgat tttaaaaact cccattcgaa tatgattcaa
      181 aattcgctta gaatcaatgt tttcccactt tttcaaaaaa taaattttca tctaatttaa
      241 ataactttca attaagtgtg aattttgctt aaaatcatta aaaaacaccc catggtgtga
      301 accatgaagt gttgtaaatg ctgtgttgta gctagttctt agcgtttaga gaactggcta
      361 gctttacgag ctttcttaag atctggtttc caaaatactt tgagtacact tatggtattg
      421 atgcccctaa acatctaaaa actctggtag aaaaagggta tgctcttgtc gaaacttctt
      481 ttgactcact tgaccatcta aatactacca tgaaaaagaa tatcctgaag aagaagggag
      541 ttactggact ttctaaaatg aaatcttgcg acctggatca agcccttcat gagcattttt
      601 cagaagaaga attagctggt cactttcatg tccttctatg gactttttta caatggcatt
      661 gctatcacac ccaataccta tctaagcgcc tggttcgtaa actttattgc agctcttcct
      721 ctaaatttcc taattgttga accaattgcc cgttttatac taagttcttt tcagaaacca
      781 tttactgggg aagaagttga agattttcaa gatgatgatg aaatcccaac tattatctaa
      841 gccagttctg taaactacta atatttgaaa tccacttcct tttagggtac aatggttata
      901 aatgaatttt tgagaggatc agaatgaaaa aactagcaac ccttctttta ctgtctactg
      961 tagccctagc tgggtgtagc agcgtccaac gcagtctgcg tggtgatgat tatgttgatt
     1021 ccagtcttgc tgctgaagaa agttccaaag tagctgccca atctgccaag gagttaaacg
     1081 atgctttaac aaacgaaaac gccaatttcc cacaactatc taaggaagtt gctgaagatg
     1141 aagccgaagt gattctccac acaagccaag gtgatattcg cattaaactc ttccctaaac
     1201 tcgctcctct agcggttgaa aatttcctca ctcacgccaa agaaggctac tataacggta
     1261 ttaccttcca ccgtgtcatc gatggcttta tggtccaaac tggagatcca aaaggggacg
     1321 gtacaggtgg tcagtccatc tggcatgaca aggataagac taaagacaaa ggaactggtt
     1381 tcaagaacga gattactcct tatttgtata acatccgcgg tgctcttgct atggctaata
     1441 ctggtcaacc aaacaccaat ggcagccagt tcttcatcaa ccaaaactct acagatacct
     1501 cttctaaact ccctacaagc aagtatccac agaaaattat cgaagcctac aaagaaggtg
     1561 gaaaccctag tctagatggc aaacacccag tctttggtca agtgattggc ggtatggatg
     1621 ttgtggataa gattgctaaa gccgaaaaag atgaaaaaga caagccaact actgctatca
     1681 caatcgacag catcgaagtg gtgaaagact acgattttaa atcttaaaaa ccaaaaaaaa
     1741 tacagtatcc acattcggta ctgtatttct tttactctca ttcttaagtt aaattattaa
     1801 aatcccatat ttggtctatc cagccttcat aaaagtctgg ctcgtggcag accataagga
     1861 tagatcccct atattctttg agagcgcgtt tgagctcatc ctttgcatcc acatccaaat
     1921 ggttggtcgg ctcgtccagc actaaaacgt tgttttcacg attcatcaag agacagaaac
     1981 gaaccttggc ttgctctccc cctgataata cttgaatctg gctttcaata tgtttggttg
     2041 tcaaaccaca acgggcaagg gctgcacgga cttctgcttg attaagggca ggaaaggcat
     2101 tccagacagc ttcaagagga gtttggcgat taccgccttc tacttcctgc tcaaaataac
     2161 caagttctaa ataatctcca cgctccactt ccccagcgat tggcgagata atgcctaaga
     2221 gactcttcaa gagagttgtt tttccaatac cattagcacc aataatcgca accttttgat
     2281 tgcgttcgaa ggtaagattt aaaggcttag taagaggacg gtcgtaacca atttgcaagt
     2341 tcttggcttg gaagataaag cgccctggtg tacgagctgg tttgaaatca aaggatggtt
     2401 ttggtttctc actttggagt tcgataatat ccatcttatc caatttcttt tgacgagaca
     2461 tagccatatt acgagttgca acacgggctt tattacgagc cacaaagtcc ttgaggtctg
     2521 caatctcttt ctgctggcgt tcgtaggctg cctctagctg agatttcttc atagcataaa
     2581 cttcttggaa ctggtagtag tcaccagagt aacgcgtcag ctgttgattt tccacatgat
     2641 agacaatatt gataacgtca ttgaggaatg gaatatcgtg cgaaatgaga acaaaggcat
     2701 tctcatagtt ttggagatag cgcttgagcc aatcaatatg ctcagcatcc aagtagttgg
     2761 tcggctcgtc caacagcaag atatcaggct tttcaaggag aagttttgcc aaaagcacct
     2821 tggttctttg cccacctgac aaagaagtta catccgtatc catgccaaag tccataacac
     2881 caagagcacg cgctacttcg tcaatcttag catccaaggt atagaaatca cgactctcca
     2941 gacggtcttg aagttctcct acttcttcca tgagagcatc aacatccgca ccgtcttcag
     3001 ccatttccat atagaggtca ttgatacgag cttcagcttt gaaaagctca tcaaaagccg
     3061 tacggagaac atcacgcacc gactgtcttt cagcaaggac agagtgctga tccaagtaac
     3121 cagccgtcac atatttggac cactcaacct ttccttcatc tggcagcatt ttaccagtca
     3181 cgatactcat aaaggttgat tttccttcac cattggcacc gaccaggccg atatgttctc
     3241 ccttgaggag acggaaggac acatcttcaa aaattgcacg gtcaccaaaa ccgtgactca
     3301 gatttttaac ttctaaaata ctcattttaa ttccttatct tgtttttatg taatcgttta
     3361 taaaggagcc aagccagata gccacccaaa gtgttggtcc acaaatcatc aatctcaaag
     3421 acgcgattga aatcaaagaa aaagtccaag attaattgcg tacactcgat tccaagactc
     3481 acaagaaaac taaaaagaag gacctttttt gttttccgca aatttggaaa tagataaagg
     3541 agttggaaaa tcagaggaaa aaacaagaag acattgagga tattttgtaa aaaaatccaa
     3601 cataattgtc caatgtcact cacttcgccc agtttccaga gagaattgaa aggagtcaaa
     3661 agaaaaacca ggagtccaag atgctgaata tctggagttc ccactcccac ggtagattgt
     3721 tcttgaggag taaagcaaaa atagacaatg caaatgctat agaaaatgac tccccagacc
     3781 aaaatatgat tataagtctt cttcatcatt aaggatttac cgctgcgact gccttctggc
     3841 ggtcacgttt cattgtgtta gagcgcaatt gtccacaagc tgcgtcaata tctgtaccat
     3901 gctcttgacg aaccacacag ttgacccctt ttttcttaag cgtatcatag aaagccaaca
     3961 cgcgctcttt gggactacgg ctatattggt catgctcact aactgggtta taaggaatca
     4021 agtttacata agacaatttc ttgatgttct tgagcaattc agtcaattcc aaggcttgtt
     4081 ctacaccgtc gttgacttca ttaagcatga tatattcaaa ggttacacca cggtttgttg
     4141 tctcaatgta gtattcaata gcagcaaaga gtttttcaat cggaaaggca cggttaagct
     4201 tcatgatact tgaacgaagt tcattgttgg gtgcgtgaag agacacggca agattgacct
     4261 gaaccccttc atcagcaaag tcacgaattt tatgagccaa acctgaggtt gaaaccgtga
     4321 tgtgacgagc accgatagcc attcctttat catcattgat agtacgaaag aaattcaaga
     4381 cattgttgta attatcaaag ggctcaccga ttcccatgac aacgatatgg ttgacgcgtt
     4441 catcctgacc acgctcagca aagtatttct gaaccagcat gatttgcgct acgatttcac
     4501 cgttattgag gtcacgttgc ttcttaatca aaccagaggc acagaaggta caaccgatat
     4561 tacagccgac ctgagtggtc acacagacag ataaaccata gtgttgacgc atgagtacag
     4621 tctcaattaa cataccgtca ggcaattcaa agagatattt gactgtacca tcagcagact
     4681 cttgcacaat accttgtttc aagggattga ccacaaactg gtcattgagc ttagcaatca
     4741 aatccttgga aaggttggtc atttcttcaa atgactgcac acgtttacgg tagagccatt
     4801 cccagatttg atctgcacgg aatttctttt ctccctgctc caatacccat tcctgcatgg
     4861 tttgatgtgt caaactatga attgaaggtt tcatttcttc tccttattct ctactcactt
     4921 ctgacgaatg acaaaatgac gttgtccctt gtcctctttc tgagaatgct ggtctttctg
     4981 acgacgtcta ttcttcttat ctgcattcga ctttcgttta gtttgagtcg gtttctttcc
     5041 ttttctagaa ggtgtttctt cttccgtctt acgcattttc ttgtcaaatg atgctcgctt
     5101 aggggcttca ttttctaaga caaaataggc acaaccataa ctacaatact ctaaaaggta
     5161 gtcttgtaaa cgactgattt tttcaagttt ttcttctgtt cggtcatcct tgtaaaaacc
     5221 tcgtaggcga agctgttcgt tgctccagtc ccccacgata taatcaaact tggttaagac
     5281 ttctgaaaaa cgctgattaa aagtcgtcac atcaaaggca tccttgatat tttcaaccaa
     5341 ggaaaaagct atcccttccg tttcgacctt atccccgtgt aaatggaact ccggaccagg
     5401 aaacttgtta tagttgtata attcaggtgc aatttctttt cgcatagata tcctttttcc
     5461 acgattactt aatactttat tctaccataa tttctagcag ttagcacgtt tctcataaaa
     5521 atgaaaaaag tctaacgatt ttgtcagacc agaatcttat aacctaaaaa agagaagaac
     5581 aattcttccc tccaactatc attatttagc agctgcgtac aattcatcta ctttattcca
     5641 gttgattact gaaaagaaag ctttgatgta gtcaggacgc acgttgcggt atttcacgta
     5701 gtaagcatgt tcccaaacgt ccaagcccaa gattggtttt ttaccttctg agattggtgt
     5761 gtcttggttt gctgttgaag tcacttcaag tttcccttct ttgttgacaa ccaaccatgc
     5821 ccaacctgaa ccaaaacgag ttgttgctgc tgcagtgaag gctgcttgga attcttcaaa
     5881 tgaaccaaat gttgcatcga ttgctgctgc cagttctgct gaaggagctg ttttctcggg
     5941 agtcatcaat tcccagaaaa gagcgtggtt caagtgtccg ccaccattgt tgataagtgc
     6001 ttgacggata tcagctggga tagattctac atcagcaagc aaggcttcaa ggtcttcacc
     6061 gatttcaggg tgtttttcta aagctgcatt ggcattgttg acataagttt gatggtgttt
     6121 gtcatggtgc aaatgcattg tttccgcatc gatgtatggt tccaaagcgt cgtatgcata
     6181 tggaagttct ggtaagataa tagccatctg taatacctct ttttctttct atatgaaaat
     6241 gataacgcaa acattcactt ttttcaagtt ttttgcttat agataagcaa atcagcaaga
     6301 aacccacgac aagataatcc tgacgtgggt tgtagtttca acaaatgtca attgacctga
     6361 ctagcaatct gtaagagtgc cttttcaaaa aggaaacctt tttcataaag acctgtctta
     6421 atctgatagt ctgtctcaat caaataggaa atagcttgct tcagaaagct caaagaaagt
     6481 cctctcgaat ctcttaatgc aaacttgatt tgataaggat ttgggttacg tcccagataa
     6541 ctacctaaac tacttgcaat ctgcgattct gtttggccag actccgccaa aatcttcacc
     6601 tgagtaaaag tccgaaattg tcccagcatg actgcaatca gtttgatttc atcttcccct
     6661 tgcaaggtca agtctctcac caaatcgcgc gcctgatcca tctttttagt cagaataaac
     6721 tgagttaaat caaaaatatt gtcctgcaag gtcttgggaa ttgcgttaac aatatcctct
     6781 tcctcgataa cagaattcgc cttataggac tgtaaaaaga gaagattttt ctggatttcg
     6841 ctaaattgaa accccgactt gatgaggaga ttttcaaaag aatgattggt aaactgcaga
     6901 ccttgtttct gactccactt ttggaaatac tggcgcaatt cttgttcttt tacttctact
     6961 gcatcgaaga ccttggcatc acgcttaagt aatttgacta accgtctttt gctatccagc
     7021 tttccttctg caaagattat caacttggtt gttggagaag gattgtcaag gtattcctca
     7081 aatgacttaa gctcatcatc tgtcaaaaag cgtttcttag cagtcgtgat atccataaaa
     7141 taatctaata tcacgatttt ctcatccgca aagaaaggaa ggctgaccaa ctccagttcc
     7201 acatccttgt aaactacttc tttcatatca aagtaggcaa agttgaggtc agcagaatca
     7261 tacccaatct gtttcaacac ttgactcttc atcacttcaa actgaccctg atctgtccct
     7321 gtaaataggc tcaggctcgg taaatttgat aaagtcaact tctgactttc ttcaatggct
     7381 agcatcgtct ctcctttctt cagatttttc gatttaattt agtcaatata gcgcaatttc
     7441 ccacggaaat cttctaagct ctcgtagcct ttttccacca tgattgcttt cagttcattg
     7501 gtaatgcggt caaaagcact gacgccttct ttgtgaaggg tcgttcccac ctgcaccata
     7561 cttgctccac agaggatgtg ttcaaaggca tctcgaccag tcagaacgcc acctgttccg
     7621 ataatttgga tttgaggatt taaacgttga taaaaggcgt gaacattggc tagagcagtc
     7681 ggtttgatgt attctccacc aattccacca aaaccattct taggccgaat aacgacagat
     7741 tcgtcttcta tatagaggcc gtttccgata gagttaacgc agttgacaaa cttgagcgga
     7801 tatttgttga aaatagctgc cgcttgatca aagtgaacaa tatcaaaata aggtggcaat
     7861 ttaattccaa gaggtttggt gaagtaagca aacacttctg ccaaaatccg gtctgttgtc
     7921 tcaaaatcat aggcaatctg aggtttacct ggaacatttg gacaggaaag atttagctca
     7981 gtcagaccac gaaaatcact ctcttggact tttttcaaaa tagtatgggt ttcctctgga
     8041 gacatgccga ccagagataa gaagaaagtt cggttcgact ctttttcctg caaatctaaa
     8101 agataatcca aataatagtc taagccatta tttggcaagc ccatagagtt gatggaacca
     8161 agtggaacat cttggtagcg tggctcagga ttcccctgac ggaagtccaa ggtcgctgtc
     8221 ttagtaacaa aggttcctgc cgctgagttt ttgacctctt ctaactcctc tatcgtcata
     8281 caagccacac ctgctgcatt catcaagcaa ttgtcaaact caaaaccagc aatttgtgtt
     8341 ttcgttgata ccatgattct aatcctccag atttctttta ttgctaatat tataccataa
     8401 ttttaattct aggtttacta gattctgtgg ttctatctat ttattcggaa aagaaagtct
     8461 agtacagaag tcatgctatg gaaacagcta gtgtattaga agtttgaaca aggcttgggt
     8521 aacagacttt tgaatgaagt ccaagcaaca aggagaaatc tcaaccatga gtccattcca
     8581 tgactctgtt gctctagaaa accttgcaat agtcaagtga taacgaataa aactttggat
     8641 tgaaatatcc ccaagattat ctattacgag gagaaatctt aacctttatc atttttaaag
     8701 aaatatagta aaatgaaaca agaacaggag aaatcgatca ggacagtcaa atcgattttt
     8761 aacaatgttt tagaagttga ggtgtactat tctagtttca atctactata catgtaaatc
     8821 tctactttcg caaaatggat tttcagaaca gtatcccagt atagttaaaa actataaaaa
     8881 agagaggacg ttcccctctt aaaatcttat tttactgctt ctttcaaagc atctacctta
     8941 tccaaacgtt cccatggaag atcaatatct gtacgtccca tgtgaccgta agccgatgtt
     9001 tgacggtaaa ttggacgctt gaggtccagc atttggataa tccctgcagg gcgaaggtca
     9061 aagatttgac gagccgcttt ttcaagttga ctttcagcta ctgttcctgt accgaaagta
     9121 tcgatacgaa cagaaacagg ttgcgcaaca ccgatagcat aggccaactg cacttctgcc
     9181 ttcttagcaa ggcctgctgc aacgatattc ttggcaatat agcgagccgc ataagaggct
     9241 gaacgatcca ccttagtcgc atctttacca gagaaggcac caccaccatg acgagagtag
     9301 ccaccataag tatctacaat aatcttacga ccagtcaaac ctgagtcccc ttgaggacca
     9361 ccgattacaa aacgacctgt cggattgata aagaattttg tcttatcatc aagataagaa
     9421 gatggaataa cttctttgat gaccttgtca atcacatctt gatggatttg ttcattagtg
     9481 gcctctggat catgctgagt agaaataacg actgtatcta cacgtaccgg acggtcattt
     9541 tcatcgtact caactgtaac ttgtgatttt gcatctggac ggagatagct aatttctcca
     9601 gacttacgaa gttctgccag acgacgaacc aatttatgac tgagtgcaat tggcaatggc
     9661 atgagctctt ctgtttcatc tactgcaaat ccaaacatga gcccttggtc acctgctcca
     9721 atcaagtcca gtggatcttg atcagcattt ccacgaacct ccaaggcttc gttaacacct
     9781 tgagcgatgt caggagattg ttccaccaaa gatgggtgta ctcccaccgt ctcagcagaa
     9841 aatccatatt ctgtattggt ataaccaatc tctgcaatgg tatcacgaac cacacggtta
     9901 atatccacat aggcatttgt agaaatttca ccaaaaacgt ggacagaacc agtatataca
     9961 gctgtttcag cagcaacgtg cgcctctgga tcctttgcta aaatagcatc caaaatcgca
    10021 tctgaaattt ggtctgcaat cttatccgga tgcccctcag atacagattc agacgtgaat
    10081 aatttacgtt ctgacataaa aatgtccccc cttaaaaaat attgttatag agttacaaag
    10141 tactgatagg aatttccatt atctaagagt tactagcttc agaaataaaa aagtatcgtt
    10201 tttatttctg aagctagtaa ggcgcatagg gtgaccgcag tatggcggca accgtagaaa
    10261 gacgagcaac ctcgaagccg tcattctttg cgaattgcaa actactgata ggaaaactct
    10321 tcgtaataaa tacctaccat cttatcggga ctatataact ttatcattat accattttta
    10381 atcgcaattt gcagtcttta atgtagtgtt tcggtttaga tagttcgttc tcataaagca
    10441 aaaagttgga gctcagtact ccaacaattt ctattaaaat gtttggcgtt tagctttacg
    10501 ctctgcacga ccgcgagcgc gattttcagc acgcttggtt ttacggcgct tttcatcaac
    10561 cgcccattga attttcttct tataacccgg tttgactttt ttctttttct ttttaaccaa
    10621 accaatcatt tcgatatcaa gtttatcttg ttttttctca cggttggcac gacgatcacg
    10681 gtcataggta tcttgaaatt ccccgtcttt gaccatctta ggactaaact tgattcccaa
    10741 tttctccaac tcacggatat ccgagtcatc acttggctga taaagggtaa tagctgtacc
    10801 tggtaggcca tttcgtccag tacgaccaac acggtgaaca aaaaaagata agtcttgcgg
    10861 aatggcatca ttgatgacat ggctgacacc ttcaatgtca atcccacgcg ctgctaaatc
    10921 tgttgcgaca atatactcaa aatccagatt ttgcacctga ttcatgattc gcttgcgttc
    10981 acgaggggca atatctccat ggatttttgc aaccttcaag ccttgagcag tcagatatga
    11041 atgcaattca tcagcacgcg ttttagtgtt aacaaaaatc attgccaaat acggctgcat
    11101 caactgagtc aactggtaaa tttgagcatt cttatcatgt cccttggtcg aaatcaacca
    11161 attatcaatg gtgtcagaaa taaccgtttt ggtcttaatt ttctccataa caggattgga
    11221 taagtatttt ttcaagaatg gttgcagttt ttgtgggata gtcgctgaga agaccatgaa
    11281 ttgcaagtct tttggaagac tgccagcaat cttatcaaca gtttccaaga atcccatatc
    11341 caaggtcata tctgcttcat caacaacaaa tgtcttggct ttatgaatag ctaaatcacc
    11401 agatttaacc aagtcgtaga tacggcctgg tgttccaata acaatatgag gctgattgct
    11461 tgccaatttc tcaatctggc gagccttatc cgtaccaccc acataattaa ccacacgaac
    11521 ttcgacatct gagtgagctg aaatctgacg cgctacttgg taaatttgag tagccaactc
    11581 acgactcggt gcagtaatca ctgcttgcac actatcgcta gcttcatcta attgctggaa
    11641 aatcggtaac aagaaagtat gagtcttacc tgaacctgtt tttgattctc ctactaggtc
    11701 acgacctgcc aaaacaatag gaatcaactt gtcttgcacc tctgttggag ttgtaaattt
    11761 taactccttc aaggcttctc taatatagtt tttaaattga aatttcgtaa atgacataac
    11821 atcctcgatt ctatctatct tatcaattat accatatttt gaacattttc agtagtccga
    11881 ctcatctgac ctatcacaag gctctcaatt tctctaaata tagtaaaatg aaataagaat
    11941 aggatgaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gcagaggtgt
    12001 actattctag tttcaatcta ctatacctct cttatactaa aaatcataca tttgctttct
    12061 ttccaaaatg caaaaagaga agaccaaatt ggtcttctct cagccttaaa gcttgcgact
    12121 cagatagatt tgtcaaacag tcaaacagtt gcttaagaat cttgtaaaag tcgaaagctt
    12181 ctagaaaaat tagtacttaa tcatctttcc gcttgcgacc aagaagcaac actgctagac
    12241 ctgcaagaag agagctagtc actagtccag tgagtgaaag tggtgcagta ctacccgcat
    12301 ttggcaatgt agcttgtttt tccttttctc ctgctggacc ttgagctggt ggattctctt
    12361 caggttttct tggttgttct gattgatgtc tatctgcctg attattatca gttccctgct
    12421 ctccccctgg attcactggt ggaactggat ttggttgagg tgctggttgt ggcgttggtg
    12481 ccggctgtgg ttgtggacta ggatcttgct tagcttcaaa gacccactta ccgacgaagg
    12541 ttagatttga cccctcttcc acttcttggt aacctaagaa tgtccatgta ccatttgcaa
    12601 cagcaacttc tacaggcatc ggtgcttctg gaacgaaagc aatcgctaca gaagaacttg
    12661 tagtatatgt tccactttgt gggagtaagc tcaatacttc tgctggcaag ttgttgtctg
    12721 cggttgcact
//
LOCUS       DAVMAF010000054        12467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMAF010000054 DAVMAF010000000
VERSION     DAVMAF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.054"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            457..750
                     /locus_tag="VYO97_001523"
                     /pseudo
     CDS             457..750
                     /locus_tag="VYO97_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            857..1208
                     /locus_tag="VYO97_001524"
                     /pseudo
     CDS             857..1208
                     /locus_tag="VYO97_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1484..2784
                     /locus_tag="VYO97_001525"
                     /pseudo
     CDS             1484..2784
                     /locus_tag="VYO97_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2830..3840)
                     /gene="tsaD"
                     /locus_tag="VYO97_001526"
     CDS             complement(2830..3840)
                     /gene="tsaD"
                     /locus_tag="VYO97_001526"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1754297.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMVAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3830..4267)
                     /gene="rimI"
                     /locus_tag="VYO97_001527"
     CDS             complement(3830..4267)
                     /gene="rimI"
                     /locus_tag="VYO97_001527"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1754298.1"
                     /translation="MIEIKRIQQRPDLAQAIYAVMAAVYPVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENLFEAEVLQIAVKEAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNHRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4264..4947)
                     /gene="tsaB"
                     /locus_tag="VYO97_001528"
     CDS             complement(4264..4947)
                     /gene="tsaB"
                     /locus_tag="VYO97_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1754299.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDFDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVAPF
                     VEQIQEYLPRTNFKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5125..5310
                     /gene="cibA"
                     /locus_tag="VYO97_001529"
     CDS             5125..5310
                     /gene="cibA"
                     /locus_tag="VYO97_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1754300.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5312..5464
                     /gene="cibB"
                     /locus_tag="VYO97_001530"
     CDS             5312..5464
                     /gene="cibB"
                     /locus_tag="VYO97_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1754301.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6282..6515
                     /locus_tag="VYO97_001531"
     CDS             6282..6515
                     /locus_tag="VYO97_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1754302.1"
                     /translation="MIYKIFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6517..8196
                     /locus_tag="VYO97_001532"
     CDS             6517..8196
                     /locus_tag="VYO97_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1754303.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRNLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8294..8371)
                     /locus_tag="VYO97_001533"
                     /pseudo
     CDS             complement(8294..8371)
                     /locus_tag="VYO97_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8575..10488)
                     /gene="mnmG"
                     /locus_tag="VYO97_001534"
     CDS             complement(8575..10488)
                     /gene="mnmG"
                     /locus_tag="VYO97_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1754304.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMILPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10498..10953)
                     /locus_tag="VYO97_001535"
     CDS             complement(10498..10953)
                     /locus_tag="VYO97_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1754305.1"
                     /translation="MTQQNFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11094..12215)
                     /gene="mnmA"
                     /locus_tag="VYO97_001536"
     CDS             complement(11094..12215)
                     /gene="mnmA"
                     /locus_tag="VYO97_001536"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1754306.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLGKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3545 a         2918 c         2464 g         3540 t
ORIGIN      
        1 caataacctt tgaatctcta gatacgatta tcaaaatagg atatagagac atccttctga
       61 tctgcttttt acaaagtcca attatatgcg gatctatacc tccacaatgt ccattattat
      121 acctaactat aatatgagcc gaaaacacta tatccttaat gtctccatat ccatcaggga
      181 tattaatatt tatttttcca caactatatt gcattgtaac catctcctta aacgacgcat
      241 tatgatattt gatagagaaa tttttatgaa taactcaata attttatagt aaatcatgct
      301 tatatctcaa agatacctat tttctcttgt ctcgaccttc tccaaagaat tgctataata
      361 ctattacaaa tacatctgca ctacacttca aattttagca ctgtataaaa acgtttcaac
      421 acactaactt taagaaaact tccactatta attgaaaaag ttgatagaga taaattaaag
      481 atctatattg aaactcatcc cgatgcttat ttgactgaaa tagctgctga attcaactgt
      541 cctccaacaa ctattcatta cgctctaaag gctatgggat atagtctaaa aaagagctgt
      601 acctactgcg aacaagaccc agaaaaagta aatcggttcc ttaaagaatt gaatcactta
      661 agctacctga ctcctattta tatttatgag acaggggttg agacctattt ttatctcgaa
      721 tatgatcgag ccttgagcag gcagttagtc tctctggaag aagatataat tatttgaatt
      781 aagatcgaga caacgcacac cagagattgc gatactgtta tagaagtact aatgcccttt
      841 tttgtttcaa tatactatgg ctccgatgac ctataaagat acgatgacga gtgacttttt
      901 cgaagcttgc ttccaaaaat tcttactacc tactttagat acaccatccc ttatcattat
      961 ggacaatgca aggtttcaca gaatgaacat gtgtaaggag cagggcatag actgttacca
     1021 cttcctacct attcacccga gtataatccc attgagaaaa tatgggctta catcaaaaac
     1081 atctcagaat aatattgtca aattacgatg cttttcttga ggcacttttg tcctattctt
     1141 gtttcagccg actatactcc gttattgggc agctacggaa cagtcgatgg gacgatgggg
     1201 ggacataaaa aaatcctcca gttttgtttt ttataacagt atactggaga attgacaatc
     1261 tcggtagata cctcgttata gcgcggttac ttattaggca gttacaaaac aactgtgaac
     1321 agaaaacatt ccagagtcag acaagacttt ggaatgtttt ggctctataa tttctgtagt
     1381 gggtaatccc accccaggaa ttatagggtc gtttcttgta gaaaaaaagc cccatatgac
     1441 ctataatgaa aagcgtctaa ccaactcatt agaaagggtt catatggaac aacttaagaa
     1501 taccacagat ttgctcggat tgaaagacaa aaatatcaaa atcttgtctg ttctgaaata
     1561 ccaaacccat ctagtcgttc aggcaaagtt ggattccccc gctcctcctt gtcctcattg
     1621 tcaagggaag atgatcaaat acgacttcca gaaagcctct aaaattccgc ttctcgactg
     1681 tcagggttta cccacggtac tgcatctcaa aaagcgccgc tttcagtgca agaattgcct
     1741 taaggtggtc gtttctcaaa catccattgt caagaaaaat tgccagattt ccaacatggt
     1801 gagacaaaaa atcgctcagc tcctccttga aaagcagtct atgactgaga ttgcccacag
     1861 attggcggtc tcaacttcca ccgtcatccg aaaactgagg gaatttaagt ttgaaaccga
     1921 ttggaccaag ttgccaaaag ttatgagttg ggatgagtat agcttcaaaa agagcaaaat
     1981 gagcttcatt gcccaagatt ttgagtccaa atccatcctc gcaattttag acgggcgaac
     2041 tcatgcggtg attcgaaacc atttccaacg ctatcagaga gaggttcggg agctggtcga
     2101 ggtcatcacc atggacatgt acagccctta ttatcggctc gctaagcaac tctttccaaa
     2161 ggcgaagatt gttcttgacc gcttccacat tgtccaacat ctgagccgag ctatgaaccg
     2221 agtacgaatc caaatcatga accaatttga ccgaaaatcc ttggagtatc gggcgctcaa
     2281 gcgcttttgg aaccctcgct ttttcgtttc taggctcggg ctaaatcagt ccactggact
     2341 gatttactac accagtatag cttcaagctc tgtcagaaac gattctatca gcccacgttt
     2401 cgaatgcact taacccatcg ggaagtacga gataagctgc tttcttactc tgagggatta
     2461 caggttcact acgaactcta tcaactcctg ctctttcatt ttcaagagaa gaatgccgac
     2521 catttctttg gattgattga gcaagaactg ccaacggttc atccgctttt tcaaacggtc
     2581 ttttggactt ttttaaggga tagagataag attatcaacg cacttaagct gccttattcc
     2641 aacgctaaac ttgaagcgac caataatttg attaagatta tcaagcgcaa agcctttggt
     2701 ttccggaact ttaacaattt taaaaaacgg attttgatga ctttgaacat caaaaaagag
     2761 agtacgaatt tcgtactctc gagattgcag cttttcgcct acccactaca cttgacaaag
     2821 agccactctt tattccatgg tatcaaaggc aagacttggt ttggcattga ggtcccagcc
     2881 tgcgaagttt tctttgttcc actcgctgac gctggcatag gcaatcatac ctgcattgtc
     2941 tccgcagagt cgcagagggg ggatgataac cttgacatct gtgatttcgg ctgctaggcg
     3001 ttctctgaga cctttattgg ctgccacacc acctgccaca actagggttt taacaggata
     3061 tttctccaaa gccttcttgg tttttgccat gagaatgtcc ataactgctg cttggaagga
     3121 agcacacaaa tcttctgtag acaggctttc tcccttttgc tcggcattgt gatgaagatt
     3181 gataaaggca gatttcaaac ctgagaagga gaactccaga ttatcttcct taatcatggc
     3241 acgggggaaa tcataaatat cctgcccctg atgagccagc tcgtcaatct cacgacctgc
     3301 aggataggtc aagcccatga cacgaccgac cttgtcataa gcctccccaa ctgcatcgtc
     3361 tcgtgtctcc ccaacaatct tgtaatcgcc agcctcagaa acatagacca actctgtgtg
     3421 cccaccactg actaaaaggg ctagcaaggg aaactccaaa ggctccacac tctgagctac
     3481 catgaggtgc ccagccatgt gattaacagg aatcagtgga agtccgtgag cccaagcaaa
     3541 ggccttggca gctgacaaac caactagcaa ggctccgacc aagcctggtc cgtaggtaac
     3601 cgcaacagct gtcacgtcct cttcggtaat ccctgcttct gccaatgcct cctcgataca
     3661 ggctgtaatg acctcgacat ggtgacgact ggctacttcg ggcactacgc caccaaaacg
     3721 tttgtgactc tcaatttgac tagcaatgac attggacaag agctcatcgt cgtttttcaa
     3781 gacggcgaca ctggtctcat cacaggatgt ctcaaatgct aaaatatatc tatccttcat
     3841 ctatttctct cttcataatg atggcgtcct cgactgggtc atggtagtag gcctttcgct
     3901 cagcgatgac tgccatcttt tctttcttgt aaaatgcttg cgctcgatga tttgactgtc
     3961 tgacttcaag gaaaatttcc ttgtctgtcg gcaattgagc aaacaaggct gacgcaattc
     4021 cctgaccctg ataggcttct ttgacagcga tttgcaagac ttctgcctca aaaagattct
     4081 cctgaacagc tagaaagcca atcacttctg ccccatcata agccagaaca taccaagtct
     4141 ggtcttggga caggtctgct tggatttgtt ccagcgtcca cggactaact gggtaaacag
     4201 ctgccataac agcgtagatg gcttgagcca agtcaggtcg ctgttggatt cgtttgattt
     4261 ctatcatagg cgtttaatgt aagactcgcc agactctgtg tggttcttga gccagttttc
     4321 ctcagcctca acacgtttga gataattcgg cacaaaatca tgcaaggagt ctgcttcctt
     4381 gtcccaagct aaaagagcaa gattggctgc atttggcaag gtttctttga aattagtcct
     4441 tggcaagtat tcttgaatct gctccacaaa gggggcaact tctccgacaa aggttacctg
     4501 actagcgccc ttgattagct caatgactcg ctcaaaaggc agatgcgctt ctggcatgac
     4561 aggtttagca ttttcataaa atcctgcata aacattgttg cgacgcgcat ccatcaaagg
     4621 aacgaacaag ccttcttgtt gatatggcac cagagccaag aggctagaca taccaactaa
     4681 ctcaatgttc agagtatgag ccaaggtctt ggcagtggct accgcaattc gcaaacctgt
     4741 gtagctacct ggcccttcag ccaccacgat tcggtcaaaa tccttgggtg tccaatccaa
     4801 acttgccatc aaaaaatcga tggcaggcat aagggtaata ctgtgatttt tcttaatatt
     4861 aatcgtcgtc tcggcaagaa cctgcttatc ctctaaaata gccagagaaa gagccttgct
     4921 ggacgtatca aaagctaata ctttcataac acattcctat ctttttgtct gcttactatt
     4981 atactacaaa agatggcaca tgggaatttt ctttgccccc agacaagagt gccctcactt
     5041 aactaaaaat aatttaaaaa aatgctcact tttccttttc ttttccgaat ataaaagtga
     5101 acaagaaaaa aggaggaaag ttcaatgaca aattttgaca ttcttgacaa tcaattttta
     5161 tccttatctg aaaatgaatt atcagatatt gatggcggtc tcgctccctt ggttatcttt
     5221 ggagtagcag tatcttggaa ggctattgca ggtggaacag cacttatagg ttctggtttg
     5281 gcagctggtt attttttagg aggagattaa tatgatgaaa gatttgaaca actatcgtga
     5341 aatttctaat aaggaattgc aagaaatcaa gggtggcttt ggtgtaggtg ttggtatcgc
     5401 tttatttatg gcaggttata ccattggaaa agaccttcgt aaaaagtttg gtaagtcatg
     5461 ctagataaga aacacatttt tagaaggata aattttattg tcttcatctc ttacagtttg
     5521 ctcagcattc tcaatgattt gaacattact actatccctt taccattcga tttatctgtt
     5581 tgtattgttt tatttttatg cttcaactct atttttgagc agaacaatga ctcccataaa
     5641 aataataagc tttgaaaatt ccattgtcat gtcatgttag aaaaatgcaa agaccacctc
     5701 atcttgatag atggggtgga attttcgtgt cgtaaatcta ctatctctac atccccaaac
     5761 aaaaaaaacc agcataagca gggcatctaa gcatttaatc caaagtaaaa tacaaaccaa
     5821 acgacatagg tcacgaggag gagaaaaagc gagtagagag tcacaaaggt cattttccac
     5881 aagaacttgg tttgtcatcg ttccagtttg gcaaatagaa gatttccttc ataaacgcaa
     5941 gcaacaaaaa cgataaaagc tagcaaacga actccgataa caaaagcaaa taagttatac
     6001 atagggcaac ctccttgact taaaatctat atggaattat gacaagtaat aaacttcact
     6061 tccgttatca acataacaca ttttctttat ttttgcaaac gcttaccgaa gaaatcgtcc
     6121 cctaactttc tcgtttccgt cttttactaa tttttcattt tgtggtataa ttgaaataat
     6181 tgtaacgaat caaggtcaat ctagacacaa aatggaatga aatcaagcaa atatctgcta
     6241 aaagtttgga ataagctgac ctgtaaatag aaaggaacta tatgatttac aaaatttttt
     6301 atcaagaaac aaaagaacgt agcccacgcc gtgaaacaac acgcgcgctt tacctagaca
     6361 tcgatgccag ctcagaactt gagggccgta tcactgctcg ccaacttgtc gaagaaaatc
     6421 gcccagagta caatatcgaa tatatcgaac tcttgtctga caaattgctc gattacgaaa
     6481 aagaaactgg cgccttcgaa attacggagt tctaatatgg cctacactct taaacctgaa
     6541 gaagtcggcg tttttgccat cggtggtcta ggagaaatcg ggaaaaacac ttacggaatt
     6601 gaataccaag acgagattat catcgtcgat gctgggatta aattcccaga agatgacttg
     6661 cttggtatcg actatgtcat tcctgactac tcttacatcg tggacaatat cgaccgcgtc
     6721 aaggctgttt taatcacaca cggacacgag gaccacattg gtgggattcc gttcctactc
     6781 aagcaagcaa atgtccctat ctacgctgga ccgcttgcct tggctttgat ccgtgggaaa
     6841 ctcgaagaac acggcctctt gcgcaacgcc aaactttacg aaatcaacca caacaccgag
     6901 ttgaccttta aaaatctcaa ggcaactttc tttagaacga ctcactctat tccagagcct
     6961 ttggggattg tcattcatac tcctcaaggg aaaatcgtct gtacgggtga ctttaagttc
     7021 gactttactc cagttggaga acctgcggac ttgcatcgta tggctgcgct tggtgaagaa
     7081 ggcgtgctct gtctcctgtc tgactcgaca aatgcggaag taccaacctt taccaactct
     7141 gaaaaagtcg ttggtcagtc cattatgaag attatccaag gtattgaagg acgtatcatc
     7201 tttgcatcct ttgcctcaaa tatcttccgt ctccagcagg caacagaagc tgctgttaag
     7261 actggacgca agattgcggt ctttggtcgt tctatggaaa aggccattgt caacggaatc
     7321 gatcttggct acatcaaagc tcctaaggga acctttatcg agccaaatga aatcaaagat
     7381 tatcctgcag gagaagttct tatcctctgt acaggtagtc agggtgagcc tatggcagcc
     7441 ctctctcgta tcgccaacgg aacccaccgt caagtacaat tacaaccagg tgataccgtt
     7501 atcttctctt ctagtcccat ccctggaaac actactagcg tcaacaagct gattaacatc
     7561 atttctgaag ctggtgtcga agttatccac ggtaaagtaa acaatatcca tacatctgga
     7621 cacggtggtc agcaagagca aaaactcatg ctctgcttga ttaagccaaa atacttcatg
     7681 cctgtccacg gtgaataccg catgcaaaaa gtccacgctg gactagcagt ggatactggt
     7741 gttgagaagg acaatatctt tatcatgagc aatggcgatg tgcttgccct tactgctgac
     7801 tcagctcgta tcgcaggtca tttcaacgcc caagatatct atgtcgatgg aaatcgtatc
     7861 ggtgaaattg gcgcagctgt cctcaaagat cgtcgcaatc tatctgaaga cggtgtcgtt
     7921 ctggcagtcg caactgttga cttcaaatcg cagatgattc tatctggtcc agacatcctc
     7981 agccgaggct ttgtctacat gagagagtct ggcgacttga ttcgccaaag ccagcgtatc
     8041 ctcttcaatg ccattcgtat cgcactgaaa aataaggatg ctagcgtgca atctgtcaat
     8101 ggtgccattg tcaacgctat tcgccccttc ctctatgaaa ataccgaacg tgaaccgatc
     8161 atcatcccga tgatcctcac accagatgaa gagtaatact cttcaaacca tgtcagcttc
     8221 gccttgccgt atatatgtta ctgacttcgt cagttctatc cacaacctca aaactgtgtt
     8281 ttgagctgac ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag caacctacgg
     8341 ctagcttcct agtttgctct ttgattttca ttgagtacaa gtcaacaaaa cagccccgtc
     8401 ttcggagctg tttttctcta tgctttcttt tgagattaaa actcaatgaa aatcagacca
     8461 gaaggctagg aaaatgcata acttgagtta gctcccatag ttcggggaac tatgggaggt
     8521 tggagatgaa tcaaagccaa gctttgaact cattcgtaag aagccgacga cgtatcattt
     8581 tgatttttga agagttttag aaatactacg atttttacct tccagataca ccatcaaaat
     8641 agaaatatct gctgggttca ctcccgaaat acggctggct tggccgatgg tttctggatt
     8701 gatgagtttg aacttctgac gagcttccgt cgcaatagaa tcgatgtcat cccagtcaat
     8761 attagctgga atgcgttttt cttccatacg tttcatcttg gcaacctgat ccatggcttt
     8821 tgaaatatag ccttcgtact tgatttctgt ttcaatcaat tcgataatct tatcatccaa
     8881 atcctctgca gctggtccga tgaaggctac cacatcttgg taagaaactt ctggacggcg
     8941 aaggaattcc ttggctgtca ctgcatctgt caagggtttg aagcccatct cctcaacctt
     9001 agcattggtt tccttgactg gcttgagttt gatactgtct aggcgtttca tctcattatc
     9061 aaattgattt ttcttgattt caaaacgagc ccagcgttca tcatccacaa ggccaatctc
     9121 gcgtcccatc tcagtcaagc gcatatcagc attgtcatga cggagaatga gacggtattc
     9181 agcacgactg gtcaagagac ggtagggttc gatggttccc ttggtcacca aatcatcaat
     9241 catgacccca atgtaaccgt cactacgctt caaaatcaac tcaggcttat cttggatttt
     9301 cagagccgca ttgatccctg cgataatccc ttggcctgct gcctcttcgt aacctgatgt
     9361 tccatttgtc tgaccagcag tgaaaagtcc tgagattttc ttggtttcca aagtcgcacg
     9421 caactggtga ggcaagatca tgtcatactc aatagcataa cctgttcgca tcatctctgc
     9481 attttccaga cctttgatgg aatgaaccaa gtcacgttgg acatcctcag gcagactggt
     9541 tgaaagtcct tggacataga cctcctctgt attgcgtcct tctggctcaa ggaagagttg
     9601 gtgacgttcc ttgtccgcaa agcgcacaat cttgtcctca atcgatggac agtaacgagg
     9661 tcccactccc ttgaccacac ctgtaaacat aggcgcacgg tggaggttgt tttggataat
     9721 ctcatgactg gtaccattgg tataggtcaa ccagcatggc acttggtcct tgacataatc
     9781 ctcatcacgt gaagtgtatg agaagtgatt aggcgcttca tctcctggtt ggatttctgt
     9841 cacgtcgtag ttgatagaag aggccttgac acgtggaggg gttcctgtct tgaaacgacc
     9901 gatttcgaga cccagttcct tgagattgtc agctaggtta atagaagcca agctgtggtt
     9961 aggacctgat gagtacttga ggtctccgat gataatttcc ccacggagag cagtccctgt
    10021 cgtcacaata acagccttag cagcatattc ttgatgggtg gctgtacgca caccgacaac
    10081 cttgccatct tccaccaaaa tctcatcaat catggtttga cgaagggtca gattttcttg
    10141 attttcaact gtcttgcgca tctccttaga gtaaagttcc ttgtcagcct gcgcacgaag
    10201 agcacggaca gctggcccct tccctgtgtt gagcatcttc atctggatgt aagtcttgtc
    10261 aatagtcttg gccatctcgc caccgagggc atcgacttca cgcacgacaa tccccttggc
    10321 agaaccaccg atagagggat tacaaggcat gaaagccagc atttcaatat tgatggtcgc
    10381 aagcaggacc ttacagccca tacggctagc ggccaaggaa gcctcaaccc cagcgtgtcc
    10441 cgcaccaatt acaataatat cgtattcttc agtaaaatga taagtcatgt ttctctccta
    10501 ttcctcaaga tgaatgtgtc ttagttggcc ttcccaatct ggtagggccg tttttaaaaa
    10561 ggctggaact agctggatat cctcaagctt atccaagtca atccactcac agggctgcct
    10621 tttctcatct tcctgcatgg tcaacggggc atcttcaagc aaatccacca gataatgaaa
    10681 ctcgatattg tgataggaaa cgccgtcttg ttcaaaacga ttttcaacca caaaagctag
    10741 ctgcccagct tgagctttga cacccagttc ttccttcact tcacggacta ccgcgtcttc
    10801 cgtgctttca ttgacttgaa tcgcacctcc aatagtgtaa tacttgccct tgtctttggt
    10861 aactagaagc ttgtgatttt ggacaatcaa ggctgtcgcc cgaacaccaa aaaccgtatt
    10921 gtctactttt gtccgaaagt tttgttgagt cattcttgtc ctttccctta aacgacacaa
    10981 aaacagtcaa aactacaaag aagtgcagga caaaaaagcc tgcaacatcc atgagctttg
    11041 accatcattt ctattgctgt tattattgta gcaaattgag aaaatttgtc aatctaaatg
    11101 tactgacaaa cttgtccatc gcggtaagca ttgtcaatca aaccaccacc tagacactct
    11161 tcgccatcgt aaaagacaac tgcctgtcct ggtgtaatcg cgcgttgtgg ttccgcaaag
    11221 atgacctctg ccttgtctcc tttgacatgg acggtcacct tagagtcagg ctgacggtaa
    11281 cggaatttag ccgtacattc tagcgtaaac tcttctggca tttcacgagt aaagtggact
    11341 tgactggctt ctaggctagt tgacatgagc gaatcatggt agaatccttg tcctacatag
    11401 agaatattct tgcttagatc ttttccgaca acgaaccaag gggcattgtc accgccgtgt
    11461 tgcccaccga taccgagtcc gccacgctga ccgattgtat agtacataag acctgcatgc
    11521 tcgcccatat cgcgaccatc cacagtcatc atgcgaccag gctgagctgg caggtagttg
    11581 ctgaggaagt ttttaaagtt cttttctccg ataaaacaaa tccctgtcga atctttcttc
    11641 ttagcagtcg aaaggcctgc ttcttctgct agtctgcgta cttcaggctt tcccaaatgt
    11701 cctagtggga acatggtttt ttgaagttgt tcttgcgaaa gttggctgag gaaataggtc
    11761 tgatccttgc cattgtccac gccacgaagc atgtgaacgg taccatcctc atcacgcacc
    11821 actcgagcat aatgcccagt cgctacatag tctgccccca aggttatggc atagtccaaa
    11881 aaggccttga acttgatttc cttgttgcac ataacgtccg gatttggcgt gcgccctgca
    11941 cggtattccg ctaggaaata ctcaaaaacg cggtcccagt actctttttc aaaattgaca
    12001 gagtagtagg gaatgccaat ctggtctgcc accgcaacca catccttgta atcttcggtc
    12061 gccgtacaga cgccgttttc atctgtgtca tcccagttct tcatgaagat accgatcaca
    12121 tcgtagccct gctccttgag caaaagagcc gtcaccgacg aatcaacacc accactcatc
    12181 cccacgacaa cacgtgtttt agagttatca ctcatggtaa gtctcccatc tattcgttta
    12241 ttcacgattg aaggtcgtgt gtgcttcacg attgaaggtc gcttgagcag tattcattat
    12301 aacacgcttg gttagagaag acaaggaaga gcttaaaaat tgttttttaa aacttatagt
    12361 caatatacag attatgtgaa tgacgtgagt ataccttctt cttatcttca ttcaaataac
    12421 tataataaat aaaacaaccc attttctcta atctattttt caagtta
//
LOCUS       DAVMAF010000055        12109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMAF010000055 DAVMAF010000000
VERSION     DAVMAF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.055"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..701
                     /locus_tag="VYO97_001537"
     CDS             <1..701
                     /locus_tag="VYO97_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754307.1"
                     /translation="LLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCL
                     DIIFPELDKIVGKHSEYTYQLLTRYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAP
                     RSIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVIL
                     AEIRNIHSFDNPAQLQAFAGLDSSIYQSGQIDLAGRMVKRGSPHLRWALIQAAKACAR
                     FSPAFKAYLKTKLE"
     gene            complement(1002..2891)
                     /locus_tag="VYO97_001538"
     CDS             complement(1002..2891)
                     /locus_tag="VYO97_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1754308.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKEQSKQLLTWIFPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            3029..4594
                     /locus_tag="VYO97_001539"
     CDS             3029..4594
                     /locus_tag="VYO97_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1754309.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4594..5334
                     /locus_tag="VYO97_001540"
     CDS             4594..5334
                     /locus_tag="VYO97_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1754310.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5443..5658)
                     /locus_tag="VYO97_001541"
     CDS             complement(5443..5658)
                     /locus_tag="VYO97_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754311.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(6542..7792)
                     /gene="ilvA"
                     /locus_tag="VYO97_001542"
     CDS             complement(6542..7792)
                     /gene="ilvA"
                     /locus_tag="VYO97_001542"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1754312.1"
                     /translation="MLSSKDIIKAHKILNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7842..8198)
                     /locus_tag="VYO97_001543"
     CDS             complement(7842..8198)
                     /locus_tag="VYO97_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1754313.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8436..8672)
                     /locus_tag="VYO97_001544"
     CDS             complement(8436..8672)
                     /locus_tag="VYO97_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754314.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSSFERAGATKSARYIAKWILRNKKH"
     gene            complement(8779..9801)
                     /gene="ilvC"
                     /locus_tag="VYO97_001545"
     CDS             complement(8779..9801)
                     /gene="ilvC"
                     /locus_tag="VYO97_001545"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1754315.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVTEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9867..10343)
                     /gene="ilvN"
                     /locus_tag="VYO97_001546"
     CDS             complement(9867..10343)
                     /gene="ilvN"
                     /locus_tag="VYO97_001546"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1754316.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(10336..12036)
                     /locus_tag="VYO97_001547"
     CDS             complement(10336..12036)
                     /locus_tag="VYO97_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1754317.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3668 a         2738 c         2098 g         3605 t
ORIGIN      
        1 aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa gtagaactaa
       61 aaatactggc tagacatatt catcgtctca agaaaaaaca gtctgattgg aaagtacaat
      121 acactcgttg tcttgatatc atctttcctg agttggataa aatcgttgga aagcattcag
      181 aatataccta ccaactcttg acacgctacc ctaatcctca gaaaaggctt gaggcaggat
      241 ttgataagct gatagaaatt aagcgattga ccgcttctaa aattcaggat atcctctcag
      301 tcgcacctcg ttctatcgga acaacatctc ctgctcgtga attcgaaatc atcgaaatca
      361 tcaaacatta caagaggctc attgacaagg cggaaacatg tgtcaatgac ttgatggctg
      421 agttcaactc ggtcatcacg acggttactg ggattggggg tcgtttaggg gcggtcattt
      481 tagccgagat tcgaaatatt cattcctttg ataatcctgc tcaattacaa gctttcgctg
      541 gactggattc ttctatttat cagtcaggtc agattgattt agctggaaga atggtcaaac
      601 ggggttcccc tcatctgcgg tgggcactca tacaagctgc caaagcatgc gctcgctttt
      661 cacctgcttt taaggcctat cttaagacta agttagaata aggaaaacat tacaatgtag
      721 ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt ttatatccta aaaaagagct
      781 gccatttgac gaacaaaaag tgatgtaatt tctgaaatta acacaggatt acctttgaaa
      841 aaatggactt tttcaagggc tttttgccgt gctctaaaat catcgccaaa tcatcaaatt
      901 gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc tttcttctat
      961 cttccattat atcatacttt ttcagtctat ggtagctctc tttaaaaggc tccgatactg
     1021 ccgccacctc cgcctccaga gaagccaccg ccagaacttc cacttccaga agatacggag
     1081 taggtacttg ctgtatttgc gacactagca taatggctca tttgcgcgct tgaatgataa
     1141 aacatactgt gccagccata agctacatag agattgatat ctggattttc aacttgaatc
     1201 tgatgaacct tcatcaaatg actaaccttg tccgcatagc caaataaggt tgcatagacc
     1261 aagagacgat tccaaacaac aatactttcc aattcagcct tatccagtcg tgcaatctca
     1321 cgtaacatat tttcaaaact ggtccagaga tagtagacct cagctcccgc ttcatttaga
     1381 acaccatcac gattatctag tcgaagcttc caataataga aaacagacaa aactaaccct
     1441 agaaaaccaa gtattggcaa agggaggtaa agatagccat gaacgtctaa actgtacaag
     1501 aacaaaccaa atccgataaa taggggcaag atagtcaaga cacccatacc cacttgcaaa
     1561 gccttttcca aaccagttaa aggacgatag taatctggga gcccccagaa ggaaactcgt
     1621 tttctcactc cttcttgcat ctggttcaat acctcttcaa aagaagattt gagttgaagc
     1681 cctcttgctt gaatccgttt ttcatcagaa actttggctc tacgataaag actatcagat
     1741 accttgtaat ccgcaaacaa attggaaaga gtttcttctt ttttacctga aaaagctaga
     1801 tttaggcagt ctttctcaaa gcttgacaaa ccatcttctt ttactagcct caaaccaact
     1861 gcatctcctt ctgaaatgat agagacattc ccacggtcta tcacatctag caaggtagct
     1921 tgaataagtt gatcaaaggt gaattttcca gctcccttga ccaagggact cacttcctcc
     1981 aaggaggtcg agtagactgc ttctgataaa accataggct ctaattccat tgggggttca
     2041 tagagacgat gatttttggc atatttgact gaaggagtgg tctttcttct ataaataaaa
     2101 tagaagcaga cactcaataa caaggagatg gaaaggatcg aagggaagat ccaagtaagt
     2161 agttgtttac tttgctcttt ttctttaacg atcgaatcct ctatcttatt aaactcttct
     2221 aaacgattcc ctttcaatcc ctgatcccta gcgctagcaa aatcggtccg aggccaatag
     2281 gcatgcaact caactccacg cttagccgga agattgtcta aacggatagt ataatcaagg
     2341 ttactctttt caatcgttcc ctctctaaaa agtttccctg tatggaaaaa gagtttttca
     2401 gcccccttgt ctccccttac atgaaattca aacttttcaa tagactctga actatctgtc
     2461 agaggttgcc aatttaattc agcgatatca tcataaagga aaagtaaatt ttttaagttc
     2521 cagacgaggt caacttcaac tatgtcgccc tcctgacctg gattatagac tctcacagta
     2581 taaccatccg cttcttctgt tacttcgcta gtcacatctg ctagttctgc accgtttttc
     2641 gcggcctgaa cctttggatg agggtcaatg tcaaacccgc taggcatctt accagcacgt
     2701 ccaagtccca cgatttgtcc cttaaagtcc tcctcaaact ggtaaactat cttctgtcta
     2761 aactctgccg tattatctgc atgaatatac aaatcccctt gataagagtt tatcttgaaa
     2821 tcaatggcaa aaacagagag tggaagaagg caaaacaagc ctaacaccag taagaaaaaa
     2881 gtttttttca tcaactaagc ccccttttta tctttcttat cattatagca ttttttcgta
     2941 agtaaaggct tacttatgtt gaaaaattac gcatttttcg ataaaatagg agacagttat
     3001 tttttaatag atcagaatag gagaaatcat gagaaaaata tacttatcta ttttcacaag
     3061 tctcttgctg atgctaggac ttgtcaatgt tgctcaagcc gatgaatatt tacgcatcgg
     3121 gatggaagca gcatatgctc cctttaactg gacccaggat gatgatagca acggagctgt
     3181 caaaatcgat gggaccaatc agtatgccaa cggatacgat gttcaaatcg ccaagaaaat
     3241 cgctaaggac ttaggtaaag aacctttggt tgttaaaacc aagtgggaag gtctagtccc
     3301 tgcccttact tctggtaaga ttgacatgat tatcgcaggt atgagtccaa ctgcagaacg
     3361 caaacaagaa attgcctttt cgagcagtta ctacactagc gaaccagttt tgcttgtcaa
     3421 aaaagattct gcctacgcaa gtgctaaatc tttggatgac tttaacggtg caaaaatcac
     3481 ttctcaacaa ggggtctacc tctacgactt gattgcacaa atcccaggtg ctaaaaaaga
     3541 aacagccatg ggagacttcg ctcaaatgcg acaagctctt gaggctggtg tcattgatgc
     3601 ttatgtttct gaacgtccag aagcactgac tgctgaagct gcgaactcta agttcaagat
     3661 gattcaagta gaacctggtt tcaaaactgg ggaagaagat acagctatcg ctatcgggct
     3721 tcgtaaaaat gacaatcgta ttagccaaat caatgccagc attgaaacca tttcaaagga
     3781 cgaccaagtt gccctgatgg atcgtatgat caaggaacaa cctgccgaag ctacaacaac
     3841 tgaagagact agcagtagtt tctttagcca agtcgctaaa attctttctg aaaactggca
     3901 acaactcttg cgtggtgctg gtatcactct tttaatctct atcgtcggaa ccatcatagg
     3961 tctcattatt ggacttgcca ttggtgtctt ccgtactgct cctctctctg aaaacaaagc
     4021 catttatggc cttcaaaaac tagtcggctg ggttctcaat gtctacattg aaattttccg
     4081 tggtacgcca atgattgttc aatcgatggt tatctactat ggaactgccc aagctttcgg
     4141 gatcaacctt gaccgtacac tggctgctat cttcatcgtt tcaatcaata ccggtgccta
     4201 catgactgaa atcgtccgtg gtggtatcct agcagttgac aagggacaat ttgaagctgc
     4261 gactgctctt ggtatgaccc ataaccagac catgcgtaag attgtcctac ctcaggtagt
     4321 ccgtaacatc ctacctgcaa ctggtaatga atttgtcatc aatatcaaag atacatctgt
     4381 attgaacgtt atctctgttg tcgaacttta tttctcagga aataccgtgg caacacaaac
     4441 ctatcaatac ttccagacat ttacaatcat cgccgtgatt tactttgtcc tcaccttcac
     4501 cgtaacacgt atcctacgct ttatcgagcg cagaatggac atggatacct acactacagg
     4561 tgctaaccaa atgcaaacgg aggatttgaa ataatgacac aagcaatcct tgaaattaaa
     4621 cacctcaaaa aatcctatgg acaaaacgaa gtgctaaaag acatttctct cactgtccac
     4681 aagggagaag ttatttctat catcggaagc tctggaagtg ggaaatcaac cttcctacgc
     4741 tccattaacc tacttgaaac gccaactgat ggacaaatcc tttatcatgg acaaaacgtc
     4801 ctcgaaaaag gctatgacct cacgcaatac cgtgaaaagt tagggatggt tttccaatcc
     4861 tttaacctct ttgaaaatct caacgttctt gaaaacacaa tcgtcgctca gacaactgtc
     4921 cttaaacgcg aacgcacaga agctgaagaa attgccaaag aaaacctgga aaaggtcggc
     4981 atgggagaac gctactggca agctaaaccc aaacaactct caggtggtca aaaacaacgt
     5041 gtggccattg ctcgtgccct ttcaatgaat cctgacgcca ttctctttga tgaaccaaca
     5101 tcagctctcg atccagaaat ggttggagag gtcctcaaaa tcatgcaaga tctggctcag
     5161 gaaggcttga ccatgattgt cgtaacccat gaaatggaat ttgcccgtga tgtctctcac
     5221 cgtgttatct ttatggataa gggcgtgatc gctgaagaag gtaaaccaga agacctcttc
     5281 accaatccta aagaagaccg aacaaaagag ttccttcaac gctatctcaa ataaaaagaa
     5341 aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc agattctcta
     5401 tctctactac ttgaaggtca agaatccgcc gttatccaaa gattaatctt caaatttttc
     5461 atgatttttt tcatagaaat caattaggcc tagggctgtt tcaaatgaaa tattttttac
     5521 ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg tttctttaga
     5581 gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt ctactttttc
     5641 aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt aattgtatac
     5701 gtttttacta cttttgtcaa taaaaaaaca tgtattcaac gaaaataatt agttcagaat
     5761 tttagcttca gaattttctt acttcctttt tgaatccgat aatatatagg ttttactagt
     5821 ctctacacca taaatatttg ctaataaaaa tactgtatta ttaccctctt aaggtgcgaa
     5881 attattcaag ttcttaaaaa atgcttctct atttccgtaa gatagcttat ttcttcgatt
     5941 taagaactga agatgatagt tctaaccttt agaaatccaa gccttcacta gctcaaaaac
     6001 tcctatcttt cttcctcaat aaatcatctg agagataggt attccttata tccttcaaat
     6061 tcatcactcc tttggatatt gaggtgctag cctagaagca gacacccgat tatctaaacc
     6121 atccttaata gagatgacaa gacagataag ctcagttaat tacttatata tctttttaat
     6181 atggtatatt tgtcaacaaa aagaaatcaa atcatacctt atactcaatg aaaatcaaag
     6241 agcaaactag gaagctagcc gcaagttgct caaaacactg ttttgaggtt gcagatagag
     6301 ctaacgtggt ttgaagagat tttcgaagag tattaaagac agtgtgacta tgcttgtcaa
     6361 tagataggaa atcaaaatga aggatggaaa tctgagccaa acatcataag tgctataatg
     6421 aacagaagta agaattgcac gataagtttc ctaaaaccat agaaatcgtc ccctttcttc
     6481 atcttcatta tagcacatat acatcagttg tacaaataat atgatatttt tttattagtc
     6541 ctcagacaag catattataa agcgtttcat taccatttaa gttaatataa gctggatcaa
     6601 aaccttccat tctacgaatc aaacctgcat aatcatgctt atctgctaaa gcgatcccaa
     6661 ttaatactgg gcctgttccc ttgctagctc gtttgatata ctcaaaacgt gtgatatcat
     6721 catttggccc caggatatca tttacaaact cacgcaaagc tcctggacgt tgtgggaaat
     6781 tgaccacaaa gtaatgtttg ataccatcat aaatcaaggc acgctcttcc atttctggca
     6841 tacggttgat atcattattt cctccagaaa tgatacaaca aatggttttc cccttaatat
     6901 attcagctaa aacctctaaa gaggcgatac tagccgctcc agcaggttct gcgactatcc
     6961 cttgcttaga gtaaaggtca atcaaggttt cagaaatcaa tccctcatcg acacctacca
     7021 aagttttaat atgttgacga gttgcttcat aggtcaactg acctaccttt tgcacagcaa
     7081 tcccatccgc aaatttatca atttccttga gtttgactgg acctccagcc tcaaaggcag
     7141 ctttcatgga acgcgctcca ttcgcctcta ctccgataac ctcaatctct ggacttgttt
     7201 ccttgatata ggtagaaacc ccggcaatga gaccgccacc accaacagga accaagacag
     7261 catcaaaatc aatcgattct tttcgagctt cttctaaaat ctcataagca actgttcctt
     7321 gacctgcttg aacatgagca tcatcaaaag gatcaataaa ggtacgattt tcagagactg
     7381 taaattcttg agctgctttg gctgaggcat caaaggtatc tccaactagt ttaatagtta
     7441 caaaatcccc accaaaaaag cgaacctgac caatcttttg ttgtggcgta gtaataggca
     7501 taaagatagt agcaggaatt ttcatttcat tacaagtata ggctactccc tgcgcatgat
     7561 ttcccgcaga agcgcagact accccacgtt cacgttcttc cttgctgagc tgggaaatgg
     7621 cataataggc accacgaatt ttaaaggagc gaacacgctg ggcattttct tttttcaaat
     7681 aaatcttagc accatacttc tccgataaat aatgatcgta atccagtgga gtattcacaa
     7741 ccacaccgtt caagatcttg tgagccttga ttatatcttt tgaacttaac atataaaacc
     7801 tctttttcta taaaaaacag tcactaatga ctgtttttta gttattcatc tccgaaaact
     7861 tcagtaacac tttcatggta tttatgagcc atacgaatcg aaaactcttt aggaagctca
     7921 taacgatttg tggaaatcca atcatataac cattctacca aactttcttc actatcatta
     7981 cgatggaaga taaaatagtt taaaatttct ttatattcta catcattcaa atccaacgtg
     8041 tctacaaatt gactacgagg aacaaaatta gaatctaatt ttcttagttg atctgtcaat
     8101 tctcccaatt gtaaatccaa ctgaaaaaat agtttttgca gttgcctatc agtttcatca
     8161 catcctgtct tgatagaaaa caaattggta atattcatat gatttcaacc cctaaataag
     8221 tacatctaat atcccacgaa tagctgaagc taaagttttc gcagtccagt catttaaacc
     8281 cgattctttc aatccagatg caataatttg ctctatttga tataaaccgc catctatcgt
     8341 ctcatttatt ctgcgaagag gaccttcaat ataggataaa gcttctccga atctaacagc
     8401 tgatttaggt cctaatgatt taccaactat atttgttaat gttttttatt tctcaaaatc
     8461 catttcgcta tataacgagc agatttagtc gcaccagctc tctcaaacga agaatgaatt
     8521 tcaagacctt cttctctagc tatttgtgcc aatttgaaga attgttcaaa ggtcatatgc
     8581 aacctaccat cttcaaaatt agcatcatca taaatgttaa ttacagtatt ttcatcatac
     8641 gaccaatctg ctttattttg gtctgctagc actattaccg acggtgtaat tacattatac
     8701 aactagaacc aataataacg atgcaatttt tctcataatc tttttcataa aatcatctcc
     8761 agcgctatat atttctaatt agttatagat tttgaatgca tcatcgtcgt ttttaccaac
     8821 gaatggcatt gctttacgca attctgcacc aactttttca atttcaaggt tagctgcttg
     8881 ttcacggtaa gcagtcaatt ttggacgtcc agctttatag tcatttacaa agtcatttgc
     8941 aaatttacca ttttggatgt ctgccaagac agccttcata ttttctttaa cttgttcagt
     9001 gattacacgt ggacctgata catagtcacc gtattcagca gtgtttgaaa tagattgacg
     9061 cattttcttg aatccacctt cgtagatcaa gtcaacgatc aatttcattt cgtgaagaac
     9121 ttcaaagtaa gccaattctg gagcgtaacc tgcttctgtc aagacttcga aacctgcttc
     9181 gataagggca gtcaaaccac cacaaagtac agcttgttca ccaaacaaat cttcttcagt
     9241 ttcttctttg taagttgttt caagaagacc tacacgagcc gctccaacac ctttacacca
     9301 gtccatagca atgtttttag catttcctgt tgcatcttgg tatactgcat aaagagctgg
     9361 aacaccaaat ccttcttcgt aagtacgacg taccaagtgt cctggtcctt taggagcaca
     9421 catgaagaca tctacatccg caggaacttt gataaattca aagtggatgt tgaaaccatg
     9481 ggcaaatcca actgcgtttc cagcttccaa gtttggagcg atttctgctt cgtacaattc
     9541 ttgttgaatt tcgtctggcg ccaagatcat gataacatca gccaacttag tagcttctgt
     9601 tactgtgtaa gtatcaaatc catcttcttt tgctttatca aaagatttac ctggacgtac
     9661 accgataata acgtcacgac ctgaatcacg caagttttga gcatgcgcat gcccttgtga
     9721 accataaccg ataacggcga tttttttacc gtcaagtgct gctactttaa catctttttc
     9781 atattccatt tgaactgtca tagcttttct ctcttttcta ttatttattg ccttttaagc
     9841 tggtttaaca aatttaagtt ggatttttaa tcgcgggtaa atccagttgc acccgttcga
     9901 gcaatattgc gaataccgta tgggcgaatg actcgcaata gggcttcgct cttttctgca
     9961 tttcccgtca tctgaatggt aatcgagctt ggcgctacgt ctaccactgt tgcacggaaa
    10021 ggttgaataa tcgctaaaat ctcagctctc ttctcagctg gcgctgacat cttaaccaaa
    10081 atcacctcgc gctccaaatg aggcttgtct gtaatatctc gaatgcgaat cacatcaatc
    10141 tgacgattga gctgtttgat gatttgctcc acttcatcat gagaagcaac atcaataata
    10201 atagtgatac gcgatacatt cggatcttct gttgctccaa cagagatgct ttcaatatta
    10261 acctgacgac gagataggac acctgtaaag cgattgagga ctcctgaacg attttgtagt
    10321 tttgctgtta acattctacg catggaactg cacccccaac atctcatgat tactcttacc
    10381 agccggtacc attggtaaca cctgttcctt acgagaaata tctacctcaa ttagcatagg
    10441 aacatcctca gtgatgactt caaggtcttg agccaaggtc tcaggattgt caaacttata
    10501 gtttttaata ccataagcct gcgccatcaa ttggaaatca ggaagggtat caaagaccga
    10561 ctctgatgtt ctgccttcat agaaggattc ctgccactgg cgaaccattc cgagtgaatg
    10621 attgttcagc ataaccacct tgattggcac cttgtaaata ttcaaaatag ccaactcctg
    10681 gttggtcatt tggaaaccac catccccaac aaacaagact acttccttat ctgggttagc
    10741 aattttagca ccgattgctg ctggaattcc aaagcccatt gttcccaaac cacctgaagt
    10801 cactaactga cgttcatttt ggtagggata atactgagct gtccacattt ggtgttgacc
    10861 aacgtctgtt accacaatgg catctccatt cgtcaattca ccaattcgtt caataactgc
    10921 ttgcggttga accacacgct ctttcttgtc ataagaacga acacgattct tgtctttagt
    10981 gactttctca atccactttt cagtattgtt gtgaactgtt ggttctgcta gcaacatttg
    11041 caaggccttc ttagcatctc caactacagg aatgtctgca ctgataatct tgccaatctc
    11101 agctgggtca atatcaatgt gggcaacctt agcattctta gcgaaagtct taggattccc
    11161 cgtcaaacgg tcatcgaaac gagaaccaat actaatcata aagtccgctt ccgtcatggc
    11221 aatatttgct gcgaatgacc cgtgcatgcc tcccattcca agaaagagtg ggtgactcgt
    11281 tgcaatcgtt ccttgtccca aaagactggt taccactgga atttgatagc gttctgcaaa
    11341 ttcatttagt tccgtagcag cctcagcata actaattcca ccaccagcta acaagactgg
    11401 ctttttagcc ttggacaatt gcttcaagat tttcttgatt tgcatatcat tcggctcaag
    11461 agtcggctga taacttggta aattcacttc tggtgaataa atgaagtctg tttctaaagc
    11521 agatatgtct tttggtaggt caattacaac tggccctgga cggcctgtag ttgcgatatg
    11581 gacagcttcc gtaatgatac gcggaatatc agctgtctca cgaacttggt aattgtactt
    11641 agtgattggc atggtaattc ccacgatgtc tgcctcctga aaggcatcct tcccaatccc
    11701 tgctcgcgcc acctgacctg taaagaccaa aaggggaacg ctatcgctca tagcatccgc
    11761 aatccctgta atggcatttg ttgctcctgg tccactagtg acgacggcaa cacccaactt
    11821 tccagttgat ttggcataac cttcagcttc atgcaaacaa ccttgctcat ggcgccctag
    11881 aatgtggcga atgcctttaa aattatatat cgcatcataa aaaggcaaaa ccgcaccacc
    11941 aggataacca aagatggtat caactcctaa atcacgaagt gtttccaaaa ctaggtccga
    12001 ccccgtctta ggagattcta aactgatttt ctccattgtt cccctttctt tcctcttaaa
    12061 aataacttgt tactatcata ccattttttc aaaatttttc aagataaaa
//
LOCUS       DAVMAF010000056        12105 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMAF010000056 DAVMAF010000000
VERSION     DAVMAF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.056"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(678..1904)
                     /locus_tag="VYO97_001548"
     CDS             complement(678..1904)
                     /locus_tag="VYO97_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1754318.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWRANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1947..2639)
                     /locus_tag="VYO97_001549"
     CDS             complement(1947..2639)
                     /locus_tag="VYO97_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1754319.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTKKGLSVDQAYVEHVLPEKMHYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            complement(2812..4563)
                     /locus_tag="VYO97_001550"
     CDS             complement(2812..4563)
                     /locus_tag="VYO97_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754320.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4553..6298)
                     /locus_tag="VYO97_001551"
     CDS             complement(4553..6298)
                     /locus_tag="VYO97_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754321.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAAVEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMAGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6504..6881)
                     /locus_tag="VYO97_001552"
     CDS             complement(6504..6881)
                     /locus_tag="VYO97_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1754322.1"
                     /translation="MKCLIDGKPTLLIKNGNIDPEACRSVGLSASDVALKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(6953..7409)
                     /locus_tag="VYO97_001553"
                     /pseudo
     CDS             complement(6953..7409)
                     /locus_tag="VYO97_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7543..8370
                     /gene="xth"
                     /locus_tag="VYO97_001554"
     CDS             7543..8370
                     /gene="xth"
                     /locus_tag="VYO97_001554"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1754323.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8382..8525
                     /locus_tag="VYO97_001555"
     CDS             8382..8525
                     /locus_tag="VYO97_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1754324.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            8525..8764
                     /locus_tag="VYO97_001556"
     CDS             8525..8764
                     /locus_tag="VYO97_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1754325.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9147..9242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9642..10223
                     /locus_tag="VYO97_001557"
     CDS             9642..10223
                     /locus_tag="VYO97_001557"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1754326.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGRVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10223..11485
                     /locus_tag="VYO97_001558"
     CDS             10223..11485
                     /locus_tag="VYO97_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1754327.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11572..>12105)
                     /locus_tag="VYO97_001559"
     CDS             complement(11572..>12105)
                     /locus_tag="VYO97_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV1754328.1"
                     /translation="KSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIV
                     QSYLEAEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLI
                     EVNFYSGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILP
                     FLYNINDLEHNILKNLK"
BASE COUNT         3596 a         2645 c         2412 g         3452 t
ORIGIN      
        1 aacaaatacc ttgtcataca agcattcgcc agggatattt acatgaatat cgctcatatg
       61 gcggattgaa actaaaacac tacaatatat ttcttaaata gaccttctat aaaagctaga
      121 tttaacctcg ataaaaatag atgcagtgct tctaaaatac tggaataggg catataggta
      181 atagtagaca agtgttttat agattgtctc gcaggagatc taatgatgta tagcaagcat
      241 cactaaacat atagaatatt tgcattatta taatatcaac atgttataaa ttctatcatg
      301 caaaagaatg actagaacat gaaagacagg cacaaaactt tgaacaatat tctgaaaaca
      361 aaaaaaagac gcgagtttcc acaatctcac tcatggaaac tcacgtcttt caaaaaatag
      421 ggtgaattga aaactagaac aagacactag attttctaaa tgatgtctag taaggattct
      481 aaatgctaca ccaaattgct cacatcttat ttcaaaccac gggactccca caagaaagga
      541 aaatcctata aaaattctct ctctgaggag aaagaggcct caactacagg tgtaaactat
      601 tatcaattga aaacaacagt ttttacactc aattagactt tttagggata ggatcacttt
      661 cgcttgactg tagtttgtca tttttttgat aaagttagca ctttttcaga aactgttttg
      721 aaagtctcaa tgatatagtc tacttcttca tcgcttaatt tagtatgaag agggagggta
      781 atttcattct caaagaaggc ataggcttta ggatagttcg tcatatcaaa tccaagattc
      841 ttataggctg tcaagagagg aagcggtttg tagtgaacat tacttgcaat tcctgcttta
      901 gccaattctt ggatgatgag gttgcgttct tctaggcttg ctccttctac acgggtgatg
      961 tagaggtggc gtgaagattc gacagtttca gtcttgtgtg ccaaaggatg gatgcgagaa
     1021 cctgcaaaac cactatcata gcggtccaca atgtccttac ggcgttgcaa caaacttgga
     1081 tagcggtcca attgtaccaa accaagtgaa gccatgatat cggtcatgtt gcacttatag
     1141 gctggtgtaa cgatatcgta ttcccatgac cccagttgca tcttggcaag agcatcctta
     1201 gtttgcccgt gaagggaaag gatttggaat tccttgtaca tctcttcgtc atcaatcact
     1261 ggattggctc tccaagtcgc acttccacct tctgccgttg taaagttctt aacggcatgg
     1321 aatgagaagg aagtaaagtc agcgatagaa ccagaaggtt gtcctttata agtagatccc
     1381 aaagcgtggg cactatcaga gacaatgaca atacggttaa aggccttttg ccacttgctt
     1441 gaagcagtaa agaagtcacg ttttttctcc acgacttgga acaaacggtc ataatcgcaa
     1501 acaatccctg cgagctctac tgggataatc accttagttt tctcagtgat agcttgctca
     1561 agcaggtcat agtccatctc aaacgtatct gcttggatat ccaccatgac aggggttgct
     1621 cccacgtgcg taatgacact acatgaagcc gtataggtca tggctggaac gatgacttca
     1681 tcaccaggtc ccacttccaa aacgcgtaaa atcaactcca gagcggctgt cgcagagttg
     1741 agacaaacag tcttaggtgt ctgtgtgtaa agagacaagc ggcgctccag ttcttttgtt
     1801 ttaggacctg ttgtgatcca accagaacgc agggtatccg ctacttcagc aatttctgct
     1861 tctgtgatat caggcggtga aaatggaata ttgtaatttg gcattgattt gctcctttta
     1921 tctgtactca ttttcttatg actactttat tttagtacct caaatacggt ttgaaacatg
     1981 attttgatgt ccccaaggaa actaaactct cggagatagg cgaggttata gtgcatcttt
     2041 tcaggaagaa catgctccac ataggcctga tcaactgaca gacctttctt cgtcatttga
     2101 ctgatgatgg tgtcctcatc cttgtagttg atgctggctg gagaggtaat ccctgcttgc
     2161 aagagcaagg ttgccatcat ttcagggcta tactgctctg tataacgtgg cacttcaggt
     2221 cgtgtaccga caaaggacat ctcaccttta aggacattga ccaactgagg cagttcgtcc
     2281 aaacggacac gtcggatgaa atttccaacc ttggtaatac ggctatcgtt agcagaagtc
     2341 accagacttc cttttttatc cgcatccgtc accatggtac ggaacttcca aatcttgaac
     2401 cgacggttgt actgggtcac acgctcttgc ttgtaaatca ctggcccctt actatccaac
     2461 ttgatccaaa tgctcaagat gagaaagatg ggagaggtta gaaccagtaa gaccaaggcc
     2521 aaaaaccagt ccaagcaacg cttgaaaatc agcgaaccct tccttttaga gacaagctgg
     2581 tagtaagact caacctcgct tgatttcatt tccacaggca agtcttccca tttcagcatg
     2641 ctgttctcct ttgtttttat tatactattt gtcaacattt ttcattatac cacaaaatgg
     2701 aaaaggcggt gaaagaaatc tgtaatttga aggatttcct tctagtactc aatgaaaatc
     2761 aaagtacagc tttgagattg cagataaagc tgacatggtt taaagagatt ttcaaagagt
     2821 ataggccatg gcccctgcct gcaaactata catcttgtga taggttcctc ccagagccaa
     2881 gagttcctca tgggttccac tctcgataat gcgtccctta tccaagacat agatgcagtt
     2941 ggcgtcttga atagtagaaa ggcggtgagc gatagcaata gttgttcggc cctgtctcat
     3001 cttcgccaga gaagcttgaa ccaagctttc tgtttcagag tcaatattgg ctgtcgcttc
     3061 atccaaaatc aggattttag gctggctggc gactgttcta gcaaaggcaa gaagctggcg
     3121 ctgcccagta gagaagctcg aaccacgctc ggaaacaggg gagtcgtacc cctgaggaag
     3181 ttcttgaata aaggaatctg catccacaaa ggctgccgca gcctgaacct gctcatcact
     3241 gatttcttgg tacatggcga tattggactt aatagttcca tgatagagga agggttcctg
     3301 taaaaccaaa ccgatgtttt ttctcagctc ttcttgactg aaatccctga tatccacatc
     3361 atccaagaga actctccctg actggaattc ataaaagcgc atgaggacat tgataataga
     3421 cgatttccct gaacctgtat gacctacaaa ggcaatggtt tcacccttat taacagagaa
     3481 agaaatgtca tccagaatcg gatgtttacc gtcatacgag aaacacacat gttcaaaacg
     3541 gatattgcct tctttgactt tagcctgccc attttcttga agaggttcat aggtcctctc
     3601 atcgattaag gcaaagacac ggcctgcaga aaccatagac gtttgcagag ttgaaaagtt
     3661 ttgcgtcacc tcaatcaagg ggtcaaaaag gcggttgatg tactggataa aggcatacat
     3721 ggtcccgacc gttatcccga tagaaagacc acgatagcca aaataggcca tcaagacagc
     3781 ataacctaga agtttcaaca aactcatggc aggtctcaaa aagagggcat ccaaggctac
     3841 agaacggttg gcatagacca agtgctcttg gttgatttca tcaaattctg cctgcaggcg
     3901 cttctcttga ttaaaggcct ggattattct gattccctcg atattctctg ccagcttact
     3961 attgatatct gacaagagac ttctggtttt ctcgatgatt ttcactgact tttttcgata
     4021 gagattgacc aaaaggaaaa tcaaagggag aaagagcaag actaaagccg tcaaacgaaa
     4081 atccagcacc aacatggtat aaagggttgt cagaaagata aaaactgctg agataaagct
     4141 ggataaaatc ccagaaaaca tatcactaat cgtctcggta tcgttggtca aacgagaaac
     4201 gatagaacct gctggcgtct tgtcaaagta agacatgccc agtttctcca tattggcaaa
     4261 ggcatcccga cgaatatccc taacaatact gtaagacacg cgcgcaaaga gaagattgcc
     4321 gacatactga actacagttt gtaggatgta gagaccatag tagaccagca aaacggtaac
     4381 ggctagttgg ttaagattgc tgagatactg gtcgataaag tgggaagcca cgaggggtat
     4441 gacactttta atgaccgtcg tcgctaggag aaaactgagt gccaaaaagg tcaggagtcc
     4501 ataaggcttg agataagaca tcaagcgctt caatacagcc cattgttctt gtttattctg
     4561 catcttcttc tcctttcatt tccaactgct gagactggta ggtttgggca taccagccag
     4621 ccatcgctag caagtcttcg tgcctgcctc tttcgataat ttgaccattt tgtagaacta
     4681 aaataaaatc tgcatggaca acagcactga ggcgatgggc agtgataatg gttgtcttgt
     4741 cctttcgcgt ctccttgagg ttgtcgataa tcgcatactc tgtcttggca tctacggcgg
     4801 ataaggaatc atctaaaatc aagatatcag ggtctaaaat catagcccga ctcatagcca
     4861 accgttgctt ttgaccacca gaaagactga ctcctttttc accaatcagc gtatcaaatc
     4921 cttgaggcat gtctacaata tcttggtaaa ctcgggctag cttagtagct tccacggccg
     4981 ctgaaagggg caagttagga ttgccaaagc ggatattgtc taggattgaa gttgcaaaga
     5041 gaaactggtc ctgaggaaca tagcccatga gactgcgaag gtctgtcaga cgatagtccc
     5101 gaatatcgtg accgtttaga taaatggcac ccttatccac atcgtattca cgcaagagga
     5161 gcttgattaa ggacgttttc ccagagcctg tctgcccaac caagcccagt gtttgccctt
     5221 ttgccaaact aaagtgaata tccgtcagtg tttcctcatt ttcaaaagca aagctgtcaa
     5281 tggtatactc caaacgccca ttttcaatac catccagagg aaactcaggg tcttgtacag
     5341 gagattcctg agacaaaaga ttttcaatcc gctggtaaga aaccttccct cgctgagtag
     5401 tattaaagag gaagccgatg gccataagag gccagaccag catatccaaa tagctgataa
     5461 aggtgactag attaccaact gtaatctgcc cttcctgaac catcaaggag ccaaccaaaa
     5521 gcgttaaaac ataggacgaa ccaacaaaca agagaaccat agggtcaaag agactatcat
     5581 atttcatggt ttgcaggttc ttttggaagg ttaattcatt gactgcctga aaagacttca
     5641 actcgtctgc ctgataaccg aaagacttgg tcactttgat acctgatacg gactcctgta
     5701 ccttgttatt gagttcagaa aaagcagctt gggattcgcc aaaggcctta tgagtctttc
     5761 tccctaggcg actagtcgca taggccatga aaggtagggg gagaatggca acaagagtca
     5821 tctgccatga gatgctaaag agcatggtca acaaagtcac cagagccgtg atagaggcat
     5881 ccaccgcaga catgacaccg ccacctgcta aacgagtcaa ggcattgata tcattggttg
     5941 cgtgtgccat cagatcaccc gtccgatagg tttgataaaa ggctgacgac atttttgtga
     6001 aatgcttaaa caagcgagac cgcatgatct gtcccaagca ataagaggtc ccaaggatat
     6061 acatacgcca cacatagcgc aaatagtaca taccaaaggc tgcaagtagc aagtaaaata
     6121 ggctaagaag gaggtcctgc tgggttaatt gccccgatgt gatggcatca atgactcgcc
     6181 ccataaccat aggaggaatg agattgagga cggaaaccaa gaccagggcc acaatcccga
     6241 ctagataacg gcgtttttct aacttgaaaa accaccagag tttttgaata atggacataa
     6301 aatccctttc tgattgcaaa tggaaacctg aggccaatac tcaatgaaaa tcaaagagca
     6361 aactaggaaa ctagccgcag gctgctcaaa gcactgcttt gaggttgtag atagaactga
     6421 cgaagtcagt aacctacata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
     6481 gagtacaaga ccccaggttt ttcttattta taagttacga ctgtaacagc acccttgtca
     6541 tattcagcaa taaagatatt ggctacattg tcatgccctt gtttactgag gttatcaagc
     6601 aaccactctt cgctacgacc aatcgattcc aagacatcta cttgaatcac accgtcagtc
     6661 acaactggat acttaggatt ttcatctccc atttgcacaa cgatgagttg cccattttgc
     6721 tcttgcacag ctcgtttgac ttgcttcatc tggaaaatcc cttggctacg aagtttgagg
     6781 gctacatccg atgcagacaa accaactgaa cgacaggctt ctgggtcaat attcccattt
     6841 ttgataagga gagttggctt accatcaatc aaacatttca caaagtgaac attgttgttg
     6901 agctatttga gagtcaagac cagtttgatt aaaatttcga tataattaag tgtcatttgt
     6961 tcacatccac cagttcaatt gcgtctgtct tatacaattc gactttctct aacaggtact
     7021 tgtctggctc acttccgttt agggcacgat aatagcgatc tcccaccttg ataagtgcgc
     7081 catctgtgtt tgtggaagta ttaacataga cttctgactt gtctactccc aaatctttgg
     7141 aaacaacctc gatgaaatga agtgaagttt gaaatttatt gttagaaact tgattgtttt
     7201 gataagtcgt gatgctgacc aaaagcagag ctaaataagg ccagagctga aatcatgacc
     7261 aactcacgaa attcggttcc ctttttatca tggtatgcct taaaagcaaa aaaaactgtc
     7321 acagctaata gaacaatccc aaagccaacc atgataccat tttgctgacc gatttggctg
     7381 agtacatagt cataagagta gaatttcatg tattttcact ccctttcata aaatcattct
     7441 taattataaa cgaaagagag taatttttca aactatacgt tggggaaata gatctttttt
     7501 tggtataata aaatctataa tctgaatgaa aaaggtaact ttatgaaact tatctcatgg
     7561 aatattgatt ccctaaacgc tgccctaact agtgactcag ctcgtgccaa attgtcccaa
     7621 gaagtcctac aaaccttggt cgctgaaaat gctgatatca ttgctatcca agaaaccaag
     7681 ctttctgcca aaggacctac aaagaaacac gtggaaattt tagaagaact cttcccaggc
     7741 tacgaaaaca cgtggcgttc ttcccaagag cctgcccgta aaggctatgc tggaaccatg
     7801 ttcctttata agaaagaact tacacctacg atcagcttcc cagaaatcgg tgccccttct
     7861 accatggact tggaaggccg tatcatcact ctagaatttg atgcattttt cgtaacccaa
     7921 gtttacactc caaacgctgg tgacggtctc aaacgcttgg aagaacgcca agtctgggat
     7981 gttaaatatg ctgagtattt ggctgaacta gacaaagaaa aaccagtcct tgcgaccggt
     8041 gactacaacg tagcccacaa tgaaatcgac cttgcaaatc ctgctagcaa ccgccgttca
     8101 cctggattta ctgacgagga acgtgctgga tttaccaacc ttttggcaac tggatttaca
     8161 gacaccttcc gccatgttca tggcgatgtt cctgaacgct acacttggtg ggcacaacgc
     8221 agcaaaactt ctaaaatcaa caatacaggc tggagaatcg actactggct cacaagtaac
     8281 cgtatcgctg acaaggtcac taaatctgat atgattgact caggtgctcg ccaagaccat
     8341 acaccgattg tcttggaaat tgacttgtaa ggattttcta tatggactac aatgcggtca
     8401 ttcccgagtt tcttgtatcg aatatcgaac agtcccggtc cttctactgt ggtttactgg
     8461 gttttaggat tgaataccaa cgtccagaag aaaactttct ctttctcttg aagagtgtca
     8521 actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctacc cctttggtcg
     8581 tggagtcaat ctctcctttg gcataaagga tgttccaaaa ctctatcaaa aagtgatgga
     8641 ggcaaactat cctatttatc gtcccttgac caaaagaaag tttcgtgtca gtgatcccta
     8701 tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa gatttagcga
     8761 gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat tgtcaggtgt
     8821 ctaaaaactt gatagtatgc cttttatgat agaaaaatct tctaatactc ttcgaaaatc
     8881 tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactgactc tgtcaattct
     8941 atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt gctctttgat
     9001 tttcatttag tataaatgtt agcttacaat ggagtttttg ttcaccccct cttctgtttg
     9061 ttcgtttttt aaaccaaaac actaacaaaa tagttgattt ataaagttat ttagtgtaaa
     9121 ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt ttatgtcgtg
     9181 aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag tcagcagtga
     9241 gatatggccg tttttgccat gtctatttag gggacttact tagagattaa gtaggtcttt
     9301 tttgaatatt tttaatattt cccttaatta gtgcggacgg gaggtgaaat tatcctgtga
     9361 ataatttcag gaatccaggg aatttcatcc ctaatttttg agcccctagc tctttcattt
     9421 ctaggcttag gctaaaatag ttccccgaac tattttactc tcaaaaatcc cgtcatagac
     9481 aataacaaga ttattgtcta tgataccact acaacgggaa atatttgata agactcgctt
     9541 cgctcgtaat agttttatca aatatatagt attagcaaaa gggatcacta tagaaatatt
     9601 taaacaacaa gtaaattaac ttttagactt taggaggtct tatgaaatta ttagaagagc
     9661 gcatcctcaa ggatgggcat atcttgggtg ataacatcct caaggtagat tcctttttaa
     9721 cccaccaagt tgactttagc ttgatgcgag agattggtag ggtttttgcg gaaaaatttg
     9781 ctgctactgg cattaccaag gtcgtaacca ttgaagcgtc gggtattgcc ccagccgttt
     9841 ttacagctga agccttaaac gttcccatga ttttcgccaa aaaagctaag aacatcacca
     9901 tgaacgaagg catcttaact gctcaagtct actcctttac caaacaggtg accagcaccg
     9961 tttctatcgc tggaaaattc ctctcaccag aggacaaggt tttgattatc gacgatttcc
    10021 ttgctaatgg ccaagctgct aaaggcttga ttcaaatcat cgaacaggcc ggtgccacag
    10081 tccaagctat cggtatcgtg attgagaaat ccttccaaga tggccgtgat ttgcttgaaa
    10141 aagcaggcta ccctgtccta tcacttgctc gtttggatcg ttttgaaaat ggtcaggtcg
    10201 tatttaagga ggcagatctc taatgcaaac tcaagaaaaa cactcgcaag cagccgttct
    10261 tggcttgcag cacttactag ccatgtactc aggatctatc ctggttccca tcatgattgc
    10321 gacagccctt ggctattcag ctgagcagtt gacctacctg atttctacag atatcttcat
    10381 gtgtggggtg gcaaccttcc tccaactcca actcaacaaa tactttggga ttggactccc
    10441 agtcgttctt ggagttgcat tccagtcggt cgctcccttg attatgattg ggcaaagcca
    10501 tggtagtggc gctatgtttg gtgcccttat cgcatctggg atttacgtgg ttcttgtttc
    10561 aggcatcttc tcaaaagtag ccaatctctt cccatctatc gtaacaggat ctgttattac
    10621 cacgattggt ttaaccttga tccctgtcgc tattggaaat atgggaaata acgttccaga
    10681 gccaactggt caaagtctct tgcttgcagc tattactgtt ctgattatcc tcttgatcaa
    10741 catctttacc aaaggattta tcaagtctat ctctattttg attggtctgg ttgttggaac
    10801 tgccattgct gctactatgg gcttggtgga cttctctcct gttgcggtag ctccacttgt
    10861 ccatgtccca actccactct actttgggat gccaaccttt gaaatctcat ctattgtcat
    10921 gatgtgtatt atcgcaacgg tgtctatggt tgagtcaact ggtgtttatc tagccttgtc
    10981 tgatatcaca aaagatccaa tcgacagcac gcgccttcgc aacggttacc gcgcagaagg
    11041 tttggccgta cttctcggag gaatctttaa caccttccct tacaccggat tttcacaaaa
    11101 cgttggtttg gttaaattgt caggcatcaa aaaacgcctg ccaatctact acgcagctgg
    11161 tttcctggtt ctccttggac tgcttcctaa gtttggtgcc cttgcccaaa tcattccaag
    11221 ctccgtcctc ggcggtgcca tgctggtgat gtttggtttt gtatctattc aagggatgca
    11281 aatcctcgcc cgagttgact ttgctaacaa tgaacacaac ttccttatcg cagctgtttc
    11341 aatcgctgca ggtgtcggtc tcaacaacag taatctcttt gtcagcatgc cgacagcctt
    11401 ccaaatgttc ttctcaaacg gaatcgtcgt agccagccta ctcgccattg tcctcaatgc
    11461 cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt tgtttatcac
    11521 aaattgatta taaatacaat caatcccaat tcccatctaa ttgaattgga cctatttcaa
    11581 attctttaaa atattatgtt ccaaatcgtt aatattatat aagaatggta aaatatcaaa
    11641 tgtttctcgt aaattattct tagccttata ccaaccttgt ccatcagtaa tccacataaa
    11701 ctcaacatta ggaatagctt tagtttcttc agcaatcatt ttgtaagatc tagccgtctc
    11761 atttaatttt gaaccacttc ctgaataaaa attaacctca atcagataaa gcacttgttc
    11821 attttttatg acaaaatcaa aacgtttcac agtattccca tcattcgtaa tagcagataa
    11881 atcgacagaa aaaatttcct ctatttcatt ttgttcaatc tctttaaata aattctctcc
    11941 taaaatatac ccttcagctt caagatagct ttgaacaata ttttccatcg catctccagt
    12001 ccgatttttg cgcccattag tatccatacc aacctcaaca cctgtaacgt agtcaactaa
    12061 atttgatact aaatgatttt gcaataaatc aaatatacct gattt
//
LOCUS       DAVMAF010000057        11768 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMAF010000057 DAVMAF010000000
VERSION     DAVMAF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.057"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            112..765
                     /locus_tag="VYO97_001560"
     CDS             112..765
                     /locus_tag="VYO97_001560"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1754329.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            775..1239
                     /locus_tag="VYO97_001561"
     CDS             775..1239
                     /locus_tag="VYO97_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1754330.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1252..2520
                     /locus_tag="VYO97_001562"
     CDS             1252..2520
                     /locus_tag="VYO97_001562"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1754331.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2534..2794
                     /locus_tag="VYO97_001563"
     CDS             2534..2794
                     /locus_tag="VYO97_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754332.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2805..4166
                     /gene="pdrM"
                     /locus_tag="VYO97_001564"
     CDS             2805..4166
                     /gene="pdrM"
                     /locus_tag="VYO97_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1754333.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKPKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4320..5288
                     /locus_tag="VYO97_001565"
     CDS             4320..5288
                     /locus_tag="VYO97_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1754334.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5304..6296
                     /locus_tag="VYO97_001566"
     CDS             5304..6296
                     /locus_tag="VYO97_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1754335.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6514..7557
                     /locus_tag="VYO97_001567"
     CDS             6514..7557
                     /locus_tag="VYO97_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1754336.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVITQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7621..9306
                     /gene="lpdA"
                     /locus_tag="VYO97_001568"
     CDS             7621..9306
                     /gene="lpdA"
                     /locus_tag="VYO97_001568"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1754337.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKGDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9370..10359
                     /locus_tag="VYO97_001569"
     CDS             9370..10359
                     /locus_tag="VYO97_001569"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1754338.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLTQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VYO97_001570"
     CDS             complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VYO97_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1754339.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKIMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3491 a         2199 c         2655 g         3423 t
ORIGIN      
        1 attcaaatcg tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt
       61 ccatagccac tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac
      121 tcgtcttggc atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat
      181 cagtttatgg agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt
      241 caggctctct tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc
      301 tatcacggac caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct
      361 ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct
      421 catgatttga cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt
      481 cctgctggac aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg
      541 attcaggtgg tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca
      601 cgtaagaaga aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca
      661 agtcctttgt cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca
      721 ttcttaaaag agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct
      781 cagttagcga cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat
      841 aagaaaccca atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga
      901 ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc
      961 aagccagttc taaaaggtgt catcactttt cctgaattta caccagattt agactggtac
     1021 acctatgttt ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg
     1081 ctagaatggg ttccctatga cgaggttttg agcaagccga cttgggaagg tgaccacacc
     1141 tttgttgagt ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg
     1201 gataaattgt tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta
     1261 atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg atcaagtttg tgatgtctta
     1321 gttcaagatg ggaaaattat caaaattgcg cctgagatca cggaagaagg agcagaaacg
     1381 attgatgcta ctggtcttgt ggttgctcct ggtttggtcg atattcatgt gcatttccgt
     1441 gaacctggtc aaacacataa agaagacatt catactggtg ccctggcagc cgctgcaggt
     1501 ggttttacta ctgtcgtcat gatggctaat accagtccaa ccatttcaga tgtggagact
     1561 ttgcaagcag ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc
     1621 attactaaaa actttaatgg taaaaacttg actgacttta aggcgctctt agaagctggt
     1681 gcggttggtt tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc
     1741 atggaggaag ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg
     1801 aacggtgttc ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct
     1861 actggggtgg ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa
     1921 gcccatgttc acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct
     1981 caggggttag gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa
     2041 gcacttcttt taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca
     2101 gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac
     2161 cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt
     2221 atgactggct tagaaacatc cctgtctctc ggcttgacct atttagtaga agctggtgag
     2281 ttgagcttga tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt
     2341 gaagcaggtt acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct
     2401 gaccgccttg tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa
     2461 accttaaaag ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga
     2521 taggagttag aatatgaatt attttcgaaa acgtagggag aggcaagcga aatctaatag
     2581 tggaatttac tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc
     2641 tgaccatgcg gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg
     2701 tgcgtgttgg catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg
     2761 tctgaaactc tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt
     2821 gtttacgaga gaagttagta ttatttttaa aaattttctt cccaatcctg atttaccaat
     2881 ttgccaatta ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg
     2941 acttggctgg tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag
     3001 ggattgtgtc agccttggtg cctatcattg gtcaccatct tggtcgagga aaaaaggaag
     3061 aagttgcgtc tgatttttac caatttattt atttggcctt gggcctatct gtggtattgc
     3121 tggggatggt acttttcttg gcaccaacaa tcttgaatca tattgggtta gaagcagcag
     3181 tagcggcagt agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc
     3241 tctttagcgt cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc
     3301 tcatgctttt gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct
     3361 ttggtgttcc agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg
     3421 tcttgcttgg gatttctgtt ctggttttat ttaaacagga gaagcccaaa gccttacacc
     3481 ttgagaaacg aattccactt aatatggata aaattaagga aggagttcgt ttaggtctgc
     3541 ctattggggg aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg
     3601 ctaagttttc gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta
     3661 tgtacgcctt tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg
     3721 gagccaagcg atttgatgat gcgaaaacct atattggtct aggaagatgg actgccctca
     3781 tttttgcggc cttcacctta accttccttt acatttttag gggaaatgtg gccagtcttt
     3841 atggtaacga cccaaaattt atcgatttga cagtgcgttt tttaacttat agtcttttct
     3901 tccagttagc agataccttt gcggcgccgc ttcagggaat tttgcggggg tataaggata
     3961 cagttattcc tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta
     4021 cgctatttga ttctctaaca gattttggag cttattctta ctggatcggc ttgattatta
     4081 gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg
     4141 aatctttagc aaaatccaaa tgctaaaaag tttgtgaaaa aatattcttg acaagaaaat
     4201 cccttgtttt cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga
     4261 cagtatttgc caaaagcggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga
     4321 tgtcaacttt agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc
     4381 gcatggactt aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact
     4441 tttctgttgg tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc
     4501 tgattacctc aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg
     4561 gaatgatggc tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta
     4621 tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa tggtattgta ggtggtggta
     4681 tggggatcgc tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg
     4741 tctgcttctt tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg
     4801 cttctatctg gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg
     4861 cggatatcaa gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa
     4921 ttcctggaat gttcatcgaa gatggtaaca atgtcatcga tgtctatgaa ggatttcaga
     4981 aagctgtgga ccatgttcgc agtggcaatg gtccagtctt gattgaaagt gtaacttatc
     5041 gctggctcgg tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat
     5101 tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg
     5161 caagtgccga agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg
     5221 ttaaatttgc agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg
     5281 cagactaagg agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta
     5341 tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg
     5401 tcggtgtctt cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag
     5461 aacgcgtccg tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag
     5521 ccatgacagg acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca
     5581 tggacaatat cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg
     5641 ttccaatgac tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac
     5701 aatctctaga gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac
     5761 ctgcggacat gaaaggcctg cttaagtctt ctatccgtga taaaaaccca gttattatac
     5821 ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa
     5881 ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg
     5941 gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa ggaatttcag
     6001 ttgaaattgt tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag
     6061 tgaagaagac tggtaaggtt gttctggtca acgatgccca caaaacaagt ggctatatcg
     6121 gtgaaatttc agctattatt tcagaatcag aagcatttga ctatctagat gcaccaattc
     6181 gccgttgtgc aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga
     6241 ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gaatagagtt
     6301 acataagata agttatgtaa ataaaataaa gctgacgaga aactttgtat cttttaggtg
     6361 cagagctctt cgtcattaat atcttagatt agagcacggg ctaaaagctc ggaaaaaaga
     6421 taaatctcct tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac
     6481 gccctttgta tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact
     6541 cctgcagcta gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca
     6601 ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt
     6661 cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc
     6721 caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa
     6781 aatatcgaaa acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct
     6841 gataacgtaa caccatatgg tgaaatcgag cgtattccaa tgacacccat gcgtaaggtt
     6901 attacccaac gcatggttga atcttacttg actgcgccaa ccttcaccct aaactatgaa
     6961 gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc ttgagccaat catggaagca
     7021 actgggaaga agactactgt aacagacctt ctttcacttg cagttgttaa gactcttatg
     7081 aaacacccat acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac
     7141 tatgtcaatc ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac
     7201 aatgctgaga agatgagtct ttcagaactg gttgtagcct ttaaggatgt tattggtcgt
     7261 accttggatg gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat
     7321 ttgggaatgt ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc
     7381 cttggtgtca gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc
     7441 ccaatcatga gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct
     7501 aagtttatga aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag
     7561 caagagtatt catttttaag atatagataa aggaaggaag acatggcctt agaagtaatt
     7621 atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc
     7681 ggagaatttg taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg
     7741 gaattggaag ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact
     7801 gtccctgtaa cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct
     7861 ggagcagcat cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt
     7921 aagagcgatg atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca
     7981 gccattaaag ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt
     8041 ggaacctgct taaaccgtgg atgtattcca accaagacct accttcataa cgctgaaatt
     8101 attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact
     8161 gttgatatgg aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt
     8221 gttgcaggac ttcttcgtag ctacggagtt actgttcata aaggaattgg tacaatcact
     8281 aaagacaaga acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt
     8341 gctggtggtt caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg
     8401 actagtgatg acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt
     8461 ggagttgtcg gtatcgaact cggtcaggcc ttcatgacgt ttggttcaaa agtgactgtt
     8521 atcgaaatga tggaccgtat agttccagct atggatgcgg aagtttctaa gaaccttcgc
     8581 ttgatccttg agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt
     8641 gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag gcgatatcat cgcaagcaaa
     8701 gctcttcttt caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa
     8761 ttggatcgtg gttgtatcaa ggtcaatgaa tacatggaaa cttcagttcc aggcatttat
     8821 gcaccaggtg acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa
     8881 gtttccgctg aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct
     8941 gcagccatct acactctccc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt
     9001 gagaaatacg atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca
     9061 tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt
     9121 ggtgtacaca ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc
     9181 gaaatggaaa tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct
     9241 gaagtgatgt acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa
     9301 aaataaaacg aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa
     9361 agggatatca tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg
     9421 gaagaatacg cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac
     9481 aagccatcta tcattgttgg tcgtcaccag aacactatcg aagaaatcaa ccgtgattat
     9541 gttcgcgaaa atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac
     9601 gatttaaata acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac
     9661 ttcaagagct tctcaactcc ggttatcaat accctgactc aacttggggt taaagctgag
     9721 ttcacaggtc gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc
     9781 tatatcaacg gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc
     9841 ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc
     9901 cgtgcccgtg taactaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc
     9961 cgtgatttac tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt
    10021 tcagaagaag aattggccga aatcaatcac atcaaggata ctaagtttgg aacttgggac
    10081 tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt
    10141 aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt
    10201 gacttctttg gtattgaaga cgttgctgca gtagaagatg tccttcgtgg ggtgaaatac
    10261 gaacgcgaag atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt
    10321 attagccgtg aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac
    10381 taaccaactt ctttttttga tatttattta cataatttaa aatacgtaaa atcataaact
    10441 atctagagca ttcttttgtt catcattaac aatatgggta tagaggtcgg tgacttgtgt
    10501 gctggcatgt cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct
    10561 agttgctagt gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta
    10621 tttagcaacc attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag
    10681 agttaaaaaa agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag
    10741 ataattctct aaataaggtt tagcaaaagc agcgacattg actgagtcac gtttgccacc
    10801 ttttcgagta acatcaataa ccattatttt tagattgaga tctcttagat ctagattaac
    10861 agcttcagat aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc
    10921 tttatttttg ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc
    10981 gatataagtt agaaaacctt ctgtttcatc acctagaaag agtttttgct tgatattttc
    11041 agctctggca gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg
    11101 atagaaataa ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact
    11161 agaaagtgct gataaggttc gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt
    11221 agcattcagc aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga
    11281 catattttcc aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga
    11341 ctccaaaacc cagctgaaaa atcggtcata ttccttaagg tattcgtaca gggttgtaaa
    11401 actgtagggc acagccagct tagattggta gtattccaga acataccagg gcatgagttg
    11461 ttttagtttg tcgattcgtt ccagtaaaat ctcacgtttc atgtattttc tccataagta
    11521 agtatactag tagtatagca tagacttgta tatttttcaa gaaaattata gtttttcaga
    11581 aagatgttat aggagttttt caaagtgata aaaagactta gacctacaaa caaaacagaa
    11641 taaggaaaat ctcacccact aaacttctgt taatactaca taactgaaga gctatataag
    11701 gagctatgag atacaaaaca cattgtataa tagattgaaa ctagaatagt acatctggac
    11761 ttctaaaa
//
LOCUS       DAVMAF010000058        11651 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMAF010000058 DAVMAF010000000
VERSION     DAVMAF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.058"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            70..1467
                     /locus_tag="VYO97_001571"
     CDS             70..1467
                     /locus_tag="VYO97_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV1754340.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEAFRHGNRNRKPKHAIPDEIKERILKKYLSYETYKPNVLHFCELLAEEEGIKLSDT
                     TVRKILYKKNILSPKSHRKTKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKKVHPSR
                     PRKKFSGELIQMDASPHAWFGPETTNLHLAIDDASGNILGAYFDKQETLNAYYHVLEQ
                     ILANHGIPLQMKTDKRTVFTYQASNSKKMEDDTYTQFGYACHQLGILLETTSIPQAKG
                     RVERLNQTLQSRLPIELERNNIHTLEDANTFLLSYIQTFNEQFGNKTKLSVFEEAPNP
                     SERNLILARLAERVVDSGHHIRFQNRYYMPVEQGKEVYFIRKTKALVLKAFDGDIYLN
                     IADKIYHTKELLDHELYSKNFEQEPEQKKERRKYIPPQTHPWKLTSFKQYLHKNKKDY
                     EEFTSEELHSPQLQV"
     gene            complement(1764..2339)
                     /locus_tag="VYO97_001572"
     CDS             complement(1764..2339)
                     /locus_tag="VYO97_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1754341.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(2339..3127)
                     /gene="codY"
                     /locus_tag="VYO97_001573"
     CDS             complement(2339..3127)
                     /gene="codY"
                     /locus_tag="VYO97_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1754342.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            complement(3391..4965)
                     /locus_tag="VYO97_001574"
     CDS             complement(3391..4965)
                     /locus_tag="VYO97_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1754343.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            complement(5164..6390)
                     /locus_tag="VYO97_001575"
     CDS             complement(5164..6390)
                     /locus_tag="VYO97_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1754344.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6584..7900
                     /locus_tag="VYO97_001576"
     CDS             6584..7900
                     /locus_tag="VYO97_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1754345.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            7999..9342
                     /locus_tag="VYO97_001577"
     CDS             7999..9342
                     /locus_tag="VYO97_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1754346.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLAIRQIVRKEMN"
     gene            9342..10124
                     /locus_tag="VYO97_001578"
     CDS             9342..10124
                     /locus_tag="VYO97_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1754347.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILNQEIAEEYSDVKSKADFS"
     gene            10250..11332
                     /locus_tag="VYO97_001579"
     CDS             10250..11332
                     /locus_tag="VYO97_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1754348.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            11325..11528
                     /locus_tag="VYO97_001580"
     CDS             11325..11528
                     /locus_tag="VYO97_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754349.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
BASE COUNT         3610 a         2587 c         2173 g         3281 t
ORIGIN      
        1 tctttagtaa atcgaacgtg aaaatgtcac atttggtaga atagtcatat gaaaaggatc
       61 caactgaata tgaatgaaac gaaaaaatat cttgtgataa aagctatagc ccaaggaaag
      121 aaaacaaaga aacgggcctg tgtcgaactc aatctttctg aaagacaaat caatcgtctg
      181 ctactagcct atcaacagaa aggaaaagaa gctttcagac acggaaacag aaatcgaaaa
      241 ccaaaacatg caatccctga tgaaatcaaa gaacgtatcc taaagaaata cctctcctat
      301 gaaacatata aaccaaatgt ccttcatttc tgtgaattac tagctgaaga agagggaatt
      361 aagctctctg atacaactgt tagaaaaata ctctataaga aaaacatcct ttctcctaag
      421 tctcacagaa agacaaagaa gagagtaaga aaacaagcta aactgaacct gaatcaaccc
      481 ctagacaatc caatcttacc gacggctaaa gacttcctgg aagaccctaa aaaggtacat
      541 cctagtagac ccagaaagaa attttctgga gaactcattc aaatggatgc cagccctcat
      601 gcctggtttg gaccagaaac caccaactta cacttagcca ttgatgatgc ttctggcaat
      661 atcctaggcg cttattttga caaacaagag accttgaatg cctactacca tgtcctcgaa
      721 caaatcttag ccaatcacgg cattcccctt caaatgaaaa ctgataagag aacggtcttt
      781 acctatcagg cctccaactc taagaaaatg gaggacgaca cctatacaca atttggatac
      841 gcctgtcacc aactagggat tctccttgag accacctcta tccctcaagc taaagggaga
      901 gtcgaaagac tcaatcagac actacaatca cgcctgccta tcgaactgga acgaaacaat
      961 attcatacat tggaagatgc caatactttc cttctttcct acatccaaac ctttaatgaa
     1021 cagtttggaa acaagacaaa actctctgtt tttgaggagg ctcctaaccc ctctgaacgg
     1081 aatctcattc tagctagact ggcggagaga gtcgtcgata gtggacacca tatccgattc
     1141 caaaatcgtt actatatgcc tgttgaacaa ggaaaagaag tctatttcat cagaaagacg
     1201 aaagcattag ttctaaaagc atttgatggt gacatctacc tcaatatcgc tgacaaaatc
     1261 tatcacacaa aggagctact agatcatgaa ctctattcga aaaactttga acaagaacca
     1321 gaacaaaaaa aagaaagacg caagtatatc cctccacaaa cccatccgtg gaaactcaca
     1381 tctttcaaac aataccttca taaaaataaa aaggattatg aagagtttac tagtgaggag
     1441 cttcattctc ctcaactaca agtataatct atttttcgct aaatgggaag cttttcctta
     1501 aagaaaacca ctctatccct tacatttagg agagggaaag tgaggagtgt aagacaagca
     1561 tttcatttga taagaggctt ggagctgtta ggctggcgag ccaaggctaa gactagcctt
     1621 ggtttagcta acaaaacagc ttcaagggct cctcgtgtca aatgaaatca aaataattgc
     1681 cctcaaactc tccatttttt ggggacataa tcactttcga ttgacacact ttttttcaaa
     1741 ttttttcatt ttcaccaacc aggttactca gaaagttcat ttagattttc atctactaac
     1801 ttagctccga gtgtattttt gaaatgacct agggcaaatt gatgattttc aggccagatg
     1861 gaagcaacag ctggtttaac aatctcgatg tcatatccta gattataggc atctatagct
     1921 gtatgtagga cacagatatc cgtcaagaca cctgttaaga taacggtaga cactctacgc
     1981 tctctcaaac gaatatctag gtcagtccct gaaaaagctg agtaatggcg tttatccatc
     2041 caaaagacac gactgtctga accatgctct tgataaaaga tccccaaatc tccatataaa
     2101 ttccgtccac tcgtcccaat cagattatga ggaggaaata acttactttc cggatggaaa
     2161 caatcgtttt cttcatgagc atcaatagta aagaagatat aatctcctcg ttcaaaagct
     2221 aatcgagtta ccttgctgat ggcatccgaa atcgcctgag ctggagcacc tgctgttagt
     2281 ttcccactat cagcaacaaa atcttctgta taatcaatcg aaattaaagc ctttgtcatt
     2341 agtaatctct tttcttcact tcttcaaaaa tatctgaaat caagacctta agataggttc
     2401 ccttcattcc aagtgagcga ctttcaataa tccccgcaga ctcaagttta cgaagagcat
     2461 tgacaatcac agagcgagtg attccgatac gatctgcaat cactgacgca gtcaacttcc
     2521 cttcatttcc atttaattcc cctaaaattg ctgaaacagc acggagttcg gagtaagaaa
     2581 gggtattgac cgccatggtg acagcagtac gacgacgaat atttttctca tcttcttcac
     2641 gttggaagtt aagaagctga atcccaacaa cggtactggc aatctcaaca agaaccaagt
     2701 cctcatcttc gaatttttta tcattacgcc aaataatcaa agaaccaagg cgaatccccg
     2761 atacatgaat cggtgcaata gtcgtcaagc catctggaaa atcatctcta ctctcaacag
     2821 ggaaaatact catatcatgc tcaacaggca agtttgcttc tgtttcgtaa atcatattag
     2881 ccccttgaac gtagtcatct gggaaaatct tagtttggaa gaattgctct acgcgatctg
     2941 tatttgtttt ataacgcata aaatagccaa gcagacgtcc cttactattg ataatgcagg
     3001 cattgcaatg aataatatcc gctaactgac gcgtaatagc gttgtaagga agctcatcct
     3061 gcaactgctc ctctgagcgt ttcaaaattg atgtaatttt tctagttttt tctaataaat
     3121 gtgccatttt tcacctcgaa tttaatcgct atcattataa cataaaaacg tctctttttc
     3181 aataattatc tgaaaattcc ttattgactt gcattgactt acaatttaat taaaaaccag
     3241 aatattttta attaaattgt tccttttcta ttgacaagtt gcctattttt gtgtatcata
     3301 acattataaa agataatata ataattttat ttgtcttttc acattcggtc tccttatata
     3361 aaaaagcgat tcattttgaa ccgctttttc ttatttatcg cccttgttac gaataacaaa
     3421 gcctgtttgc ttttcgctta aagtattgcg tggtttttta ttatccttac ggtaacgttt
     3481 ttccttatca aaacgatcgt tgccacgact tccttttttg aactcatcac ggcgaccatt
     3541 gccacggcga tcacgctctc gacggtcgtc cccacgacgg cctccacgac ctcccttagc
     3601 tttaccaccg aaaccattac ctgatggttt aaacggtagt ggtttttcac gtgcaatctc
     3661 cacttctgga aggctatctg gatcttggac tgtcagactc aagatataca ttgccaattc
     3721 ttctggagta aactcagcag ccaatttgcg agcatcctta ccaaatttct caaagttggc
     3781 acgaatggtt tcatctgcaa aatcacgttc gattttcttg agagctacct gtttttttga
     3841 ttggaaggat tcttctacac ttgcaggttt gagacctttc atgcgtttct tagtcaagtt
     3901 ttcaatgatt tgaaggtaac ccatttcgtt tggagaaaca aaagtaatag attgacctga
     3961 cttaccagca cgacctgtac gaccgatacg gtgaacataa ctctcaggat cttgtggaat
     4021 atcgtagttg tagacatggg tcacacctga aatatccaaa ccacgcgctg caacgtctgt
     4081 cgcaaccaaa acatcaagat tgccattttt aaagtcacga aggacacgaa gacgtttgtt
     4141 ttggtctagg tcgccatgaa ttccttctgc acggaagcca cgaattttca aaccacgagt
     4201 caattcatcc acacggcgtt tggtacgacc aaatacaata gcgagttctg gttgtgccac
     4261 atccatgaga cgagtcatgg tgtcaaattt ttcttgttcc ttaacacgga tatagtactg
     4321 gtcaaccaat tctgttgtca attccttagc cgcaatcttg acatgttcag gggctttcat
     4381 aaactgaaca ccgatacgtt tgatggcatc tggcatagtt gctgagaaaa gcaaagtttg
     4441 acggttctca ggtacacggg aaataatggc ttcgatgtct tcaaggaagc ccatgttaag
     4501 catttcatcc gcttcgtcaa ggataagggt ttcaatgtct tgtaatttca aggccttgcg
     4561 tttaatcaag tccaagaggc gacctggagt tcccaccacg atatgggcac cagatttaag
     4621 agccttaatt tgtttttcaa tgcttgatcc gccatatact gaacggactt tgactccctt
     4681 actacgacca aagcggaaga gttcttcttg actttggaca gctagttcac gagttggagc
     4741 gatgaccaag gcttggatag tcgcttcttc tgtacggatt ttttcaaggg taggcaagcc
     4801 aaaggctgca gtttttcctg taccagtctg agcttgaccg ataacatcct tgccttcaag
     4861 ggccaaagga atagtttgtt cttggatagg actagcttct acaaaaccag ctttttcaat
     4921 ttctgctagc aaatcagcag acaagtttaa ttcattaaat ttcacgttat tcttctttct
     4981 aaaggtggtg cgaagccacc ctatagggct tagtttatac ttttcttttt atgacgtatt
     5041 ttcatataac tagatataaa atcgtgttgc ttcttttcca caaaagaaaa gtactgtttt
     5101 ctttgcaacc tatctagtat aacacaagac cagagcaaaa gatagcccca tttctacaga
     5161 aaatcatgta agcggttttt gactttcttt tttgattgaa cgacctagat aataagacaa
     5221 agccaaggcg atactgtata aaatgagaaa aacgaacaag gtttgtgtgt acgaatgagc
     5281 cattttataa gtctctgcta ataaaatagg tcccgctaaa ccagccattg cccaagctgt
     5341 taaaatataa ccatgcagag cggccaattc cttggttcca aaaatatcac tgagataagc
     5401 tggaatcaaa gaaaaaccag ctccatagca agtcatcaaa atagacatag caactacaaa
     5461 taaaacggaa tctgtaaaga gccaaagtga gagagaaaag aaaagattga caagcagtaa
     5521 tatactaaag gttagagggc gaccgatata gtcagacaaa ctcgcccaga gcaagcgacc
     5581 aaatccattg aaaatcccca aaacacccac cattactgct gcatgacttg tagacaagcc
     5641 agccatctcc tgtgccattg gcgatgccgc tgaaattaag cctaaaccac aagctatgtt
     5701 gataaagaaa ataatccaaa gcatataaaa ccgattgctt tttagagcct gatttgcagc
     5761 cattccttgc gtcaaagagg ctgttttttc tttccctgaa gaagataaaa ttgcaagctc
     5821 ttgctcattt ggacgcttaa tgaattgtga agctaggagc atgataataa agtaacttgc
     5881 tcctaaaata taaaaagttt ctacaatccc tacccctgcg atgaggtgtt gcgctatggg
     5941 actagtcaat aaagaagcaa aaccaaaccc cataatcgct aaacctgttg cgagaccacg
     6001 tttatcagga aaccatttta taatcgtcga cacaggggta atatagcctg ctcccaaacc
     6061 aagcccacct aaaatgccat aagcgagata caacaaccac agctcctgac ggtctattgc
     6121 aaatcctgct aagatatttc cacctgcgta tagaaaagca gatagacttc ccatgacttt
     6181 cggaccaaat ttttctacca aacgccccat aaatgcagcc gataagccca aacaaaagat
     6241 tgctagacta aaggcgaagg caacagaagc ctgatcccat cccgtttttt caataatagg
     6301 gttacgataa acactccagg cataagtcga acccagcatt aagtgtaaaa tgaccccagc
     6361 aaaggcaata atataacgat tcgatttcat aaaacctcct attttcgaac atttattcta
     6421 tcttatataa aaatagatag gattttagtt tatcaagctt gttaaaaaat tactagtttt
     6481 ttgtctggaa agcgttttta tctttttctg tctttttcgt agcagaattg tgcaaaagtt
     6541 tttttcttgt gctagaatga aatgcgaata ggaggaattc taaatgttaa cttatgattt
     6601 aatcgttatc ggatttggta aagctggtaa aacactagct ggtaaattgg cttcagctgg
     6661 caaaaaagtt gccctcgttg aacgtagcaa agctatgtac ggtggaactt gtatcaacat
     6721 cggttgtatc ccaactaaaa ctttgctggt tgctgctgag aaagacttgt cttttgaaga
     6781 agtcattgct accaaaaaca cgatcactgg tcgcctcaac ggtaaaaact atacgactgt
     6841 tgctggtact ggcgtcgata tctttgatgc tgaagctcac ttcctttcaa ataaagtcat
     6901 cgaaatccaa gctggtgatg aaaaacaaga attgactgct gaaactatcg tcatcaacac
     6961 tggtgctgtt tcaaacgtct tgccaatccc tggacttgct acaagcaaaa acgtctttga
     7021 ctcaacaggt atccaaagct tggataaatt gcctgaaaaa cttggagtcc ttggtggcgg
     7081 aaatatcggt cttgaatttg ccggccttta caacaaactt ggaagcaagg tcacagtcct
     7141 agatgccttg gatacattcc tacctcgtgc agaaccttcc atcgcagctc ttgctaaaca
     7201 atacatggaa gaagacggta ttgaattgct tcaaaatatc catactactg aaattaaaaa
     7261 cgacggtgac caagtgcttg tcgtaactga agacgaaact taccgtttcg acgcccttct
     7321 ctacgcaact ggacgcaaac caaatgtaga accacttcaa cttgaaaata cagatattga
     7381 actaactgaa cgtggcgcta ttaaagtaga taaacactgt caaacaaacg ttcctggtgt
     7441 ctttgcagtt ggagatgtca acggtggtct tcaatttact tacatttcac ttgatgactt
     7501 ccgtgttgtt tacagctacc ttgctggaga tggcagctac acacttgagg accgtctcaa
     7561 tgtgccaaat actatgttca tcacacctgc actttcacaa gttggtttga ctgaaagcca
     7621 agcagctgat ttgaaacttc catacgcagt gaaagaaatc cctgttgcag ccatgcctcg
     7681 tggtcacgta aatggagacc ttcgcggagc tttcaaagct gttgttaata ctgaaacaaa
     7741 agaaattctt ggtgcaagca tcttctcaga aggttctcaa gaaatcatca acatcattac
     7801 tgttgctatg gacaacaaga ttccttacac ttacttcaca aaacaaatct tcactcaccc
     7861 aaccttggct gagaacttga atgacttgtt tgcgatttaa gttgaaatct catcttaact
     7921 gacagccctc tttgggctgt ttttacttct acgaaacacc aaatctgtct tttccctctt
     7981 ttgtgatata atagaaacat gaacttaaaa actactttgg gccttcttgc tgggcgttct
     8041 tcccacttcg ttttaagccg tcttggacgt ggaagtacgc tcccaggtaa agtcgccctt
     8101 caatttgata aagatatttt acaaaaccta gctaagaact acgagattgt cgttgtcact
     8161 ggaacaaatg gaaaaaccct gacaactgcc ctcactgtcg gcattttaaa agaggtttat
     8221 ggtcaagttc taaccaaccc aagcggtgcc aacatgatta cagggattgc aacaaccttc
     8281 ctaacagcca aatcttctaa aactggaaaa aatattgccg tcctcgaaat tgacgaagcc
     8341 agtctatctc gtatctgtga ctatatccag cctagtcttt ttgtcattac taatatcttc
     8401 cgtgaccaga tggaccgttt cggtgaaatc tatactacct ataacatgat attggatgcc
     8461 attcggaaag ttccaactgc tactgttctc cttaacggag acagtccact tttctacaag
     8521 ccaactattc caaaccctat agagtatttt ggttttgact tggaaaaagg accagcccaa
     8581 ctggctcact acaataccga agggattctc tgtcctgact gccaaggcat cctcaaatat
     8641 gagcataata cctatgcaaa cttgggtgcc tatatctgtg aaggttgtgg atgtaaacgt
     8701 cctgatctcg actatcgttt gacaaaattg gttgagttga ccaacaatcg ctctcgcttt
     8761 gtcatagacg gccaagaata cggtatccaa atcggcgggc tctataatat atataacgcc
     8821 ctagctgctg tggccatcgc ccgtttccta ggtgccgatt cgcaactcat caaacaggga
     8881 tttgacaaga gccgtgctgt ctttggacgc caagaaacct ttcatatcgg tgacaaggaa
     8941 tgtacccttg tcttgattaa aaatccagtc ggtgcaaccc aagctatcga aatgatcaaa
     9001 ctagcacctt atccatttag cctatctgtc ctccttaatg ccaactatgc agatggaatt
     9061 gacactagct ggatctggga tgcagacttt gagcaaatca ctgacatgga cattcctgaa
     9121 atcaacgctg gcggtgttcg tcattctgaa atcgctcgtc gcctccgagt gactggctat
     9181 ccagctgaga aaatcactga aacgagtaat ctggagcaag ttctcaagac cattgagaat
     9241 caagactgca agcatgccta tattctggca acttatactg ccatgctgga atttcgtgaa
     9301 ctgctggcta ttcgtcagat tgttagaaag gagatgaact aatggtttat acttcacttt
     9361 cctcaaaaga tggtaattac ccctatcagc tcaacattgc ccacctctac ggaaatctca
     9421 tgaataccta cggggacaat ggaaacatcc tcatgctcaa gtatgtggct gaaaaactgg
     9481 gagcccatgt gaccgttgac atcgtttctc tccatgatga ctttgatgaa aatcactacg
     9541 acatcgcctt tttcggtggt ggtcaagact ttgaacaaag tatcattgca gacgacctac
     9601 ctgctaaaaa agagagcatt gacaactaca tccaaaacga cggtgtagtt ctggctatct
     9661 gcggtggttt ccaactattg ggtcaatatt atgttgaagc ttcaggaaaa cgtatcgaag
     9721 ggctaggggt catgggacac tacacgctca accagaccaa taaccgtttt atcggtgaca
     9781 tcaagattca caatgaagat ttcgatgaaa cctactatgg atttgaaaat caccaaggcc
     9841 gtaccttcct ctctgatgac caaaaaccgc tgggacaggt tgtctatgga aatggaaaca
     9901 acgaagaaaa ggtcggtgaa ggggttcatt ataagaatgt ctttggttcc tacttccacg
     9961 ggcctatcct ctctcgtaat gccaatctgg cttatcgcct agttactact gccctcaaga
    10021 agaaatatgg tcaggatatc caactccctg cctatgagga tatcctcaac caagaaatcg
    10081 ctgaagagta cagtgacgtc aaaagcaagg ctgacttttc ttaaacaaag gaaaatgata
    10141 tcaaagaact ccgttatctt gtcggagttt tttgtctttt cttttaccct tctcccttgc
    10201 attttctctc attttttgcc aaaatagagg ggtagaaaga aggtagcata tgtctaaatt
    10261 acaacaaatc ctaacatatc ttgaatcaga aaaactagac gtcgctgtcg tatctgaccc
    10321 cgtcacaatc aattacctca ctggttttta cagtgatccc catgaacgcc aaatgttcct
    10381 ctttgtccta gcggatcagg aacctctcct ctttgtccca gctcttgaag tagaacgtgc
    10441 aagtagcacc gtttccttcc cagtagtggg ctatgtcgat tctgaaaatc catggcaaaa
    10501 aatcaaacat gctcttccac aacttgactt caaacgtgtc gctgttgagt ttgacaatct
    10561 catcttgacc aaataccatg gtttgaaaac agtttttgag actgctgagt ttgacaacct
    10621 cactcctcgt atccaacgca tgcgcctcat caaatcagct gatgaagtgc aaaaaatgat
    10681 ggttgcaggt ctttatgctg acaaggctgt tcatgttggt tttgacaata tttctcttga
    10741 taagactgag acagatatca tcgcacaaat tgactttgcc atgaaacgtg aaggttacga
    10801 aatgagcttt gataccatgg tcttgactgg tgataatgct gcgaatccac acggcattcc
    10861 agcagctaat aaggttgaaa atgatgctct tctcctcttt gacctgggtg ttctggtcaa
    10921 tggctatgcg tcagatatga ctcgtacagt cgctgtcggc aaaccagacc aattcaagaa
    10981 agatatttac aacttgactc ttgaagccca acaagctgct cttgacttta tcaagccagg
    11041 tgtgactgct catgaagtgg accgcgctgc ccgtgaggtc atcgaaaaag ctggttatgg
    11101 tgagtacttc aaccaccgtc tcgggcatgg tatcggtatg gatgtccatg aattcccatc
    11161 tatcatggaa ggaaacgaca tggtcatcga agaaggcatg tgcttctctg ttgaaccagg
    11221 tatctatatc cctggtaaag tcggtgttcg tattgaagac tgcggtgttg ttaccaagga
    11281 tggcttcgac ctctttacaa gcaccagcaa agatttgctt tattttgatt aaactatata
    11341 gcccctatgc tttcctttca aaatatctag gggctatttt attgtcattt ttctgctatt
    11401 atgctaaaga aattggctgc aataatctaa ccctaagtgt ctggaatgat aacgagggtg
    11461 ctctccgctt ttatcaaaga caagggatga aaccccaaga aacaacaatg gaaatgataa
    11521 ttgattaaga agtcatctat caaaagatgt tagaaaaagt tcaatttcac tagaaaatga
    11581 ggaaaatctt cccacaataa aacgcatagt atcaggtgtt gaatatgtac tgccccccaa
    11641 aagttagaca a
//
LOCUS       DAVMAF010000059        11559 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMAF010000059 DAVMAF010000000
VERSION     DAVMAF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.059"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VYO97_001581"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VYO97_001581"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1754350.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VYO97_001582"
     CDS             complement(1169..1693)
                     /locus_tag="VYO97_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1754351.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VYO97_001583"
     CDS             complement(1746..1982)
                     /locus_tag="VYO97_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1754352.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VYO97_001584"
     CDS             complement(2050..2970)
                     /locus_tag="VYO97_001584"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1754353.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEIYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3564
                     /locus_tag="VYO97_001585"
     CDS             3160..3564
                     /locus_tag="VYO97_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754354.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLMIKHLKVKQ"
     gene            3780..4361
                     /locus_tag="VYO97_001586"
     CDS             3780..4361
                     /locus_tag="VYO97_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1754355.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4358..5635
                     /locus_tag="VYO97_001587"
     CDS             4358..5635
                     /locus_tag="VYO97_001587"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1754356.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLEDGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5653..6315
                     /gene="deoC"
                     /locus_tag="VYO97_001588"
     CDS             5653..6315
                     /gene="deoC"
                     /locus_tag="VYO97_001588"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1754357.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDISAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6302..6691
                     /locus_tag="VYO97_001589"
     CDS             6302..6691
                     /locus_tag="VYO97_001589"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1754358.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VYO97_001590"
     CDS             6782..7834
                     /locus_tag="VYO97_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1754359.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="VYO97_001591"
     CDS             7978..9513
                     /locus_tag="VYO97_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754360.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SIGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VYO97_001592"
     CDS             9506..10564
                     /locus_tag="VYO97_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754361.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VYO97_001593"
     CDS             10567..11523
                     /locus_tag="VYO97_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754362.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3285 a         2062 c         2653 g         3559 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc tacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa tttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtttttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatataa
     3061 tataagtgat caatttattg atgaaatagg aggtagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta tatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctattcgttt ttaattgctt tcttactaaa aaaacgatgg
     3361 gcgaaatgga ttgctgctgg tagttattgc tttttagtcc tatggattgc gacagagggt
     3421 tatttcttcc gcatgtcact agaagatttg atacgacttt ggacaagttt ggaaatcctg
     3481 acacaaacgt atcagttggg cttttatcta aatattttgt taggaatttt gttgatgatt
     3541 aagcatttga aggttaagca atagtaatca aaatgcgcct gatgatagac aataaacttt
     3601 ttgtttttat ctggactgtg atatttatag ttctaaatgg tttattattt ataagtgttg
     3661 aaagagattg aagaaaatgg cttcgtgact cgctttcttc ggtctttttc gttttattat
     3721 caatttgtaa acgatttaca atttgagtca aattatggta aaatagtgtc atgagtaaaa
     3781 tgtattatgc agaaaatcct gacgctgctc acgacattca tgagttgaga gtagagttgt
     3841 tgggacaaaa aatgaccttt ttgacggatg cgggtgtttt tagcaagaaa atagttgact
     3901 ttggaagtca actcttgctc aagtgtctgg atgtcaacca aggagagaca gtcctagatg
     3961 taggctgtgg ttatggtcca ttaggtttgt ccttggtcaa ggtttacgga gttcaggcga
     4021 ccatggttga tattaacact cgtgccctgg atttagctca gagaaatgct gaaaaaaata
     4081 atgcaaaagc gacgatattt caatctaata tttatgcaag agttcaaggg cgatttgacc
     4141 atgtcatttc caatcctcct atccgtgcgg gcaagcaagt cgttcatgaa atcatcgaga
     4201 aaagtaaaga tttcttggaa actggtgggg atttgacaat cgtcatccag aaaaaacaag
     4261 gggctccaag tgctaagtcc aagatggagg atgtgtttgg taattgtgaa atccttaaaa
     4321 aagacaaggg atactatatc cttagaagtg tgaaagaatg agagcagttg atttaataca
     4381 aaaaaaacga gacggtcaag aactgacttc aagtgaaatt gaatggctgg tagaaggcta
     4441 tgtgtcagga actgttcctg attatcagat gtctgccttt gctatggctg tttattttaa
     4501 aggaatgacg actcgtgaaa tttctgattt aacgatgaat atggttaaga ctgggcaaga
     4561 gtttgacctg tcagccattg atggtgtgaa agttgacaag cattctactg gtggtgtcgg
     4621 cgataaggta accttgattc tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa
     4681 aatgagtggt cgtggtcttg gccatacagg cggaacaatt gataaattgg agtccattaa
     4741 gggctatcaa gtagaacgta gtcaagagga ttttattcgt caggttcagg atattggagt
     4801 atctgtcatt gggcaatcag accagctggt caaagcagat aagcttctct atgcccttcg
     4861 tgatgtgacc gcaactgtcg acacgattcc tttgattgcg agttcggtga tgagcaagaa
     4921 aattgcggca ggggcggatg ctattttgct agacgtgact gtcggtgagg gtgccttcat
     4981 gaagacggtt gatgaggcgc gtgagttggc tcaaactatg gtagatcttg gtaaggttgt
     5041 tggacggaag acagtagcag tgattacgga tatgagccag cctttgggac gagcgattgg
     5101 aaatcgtctg gaaatccttg aagcattgga gattttgcaa ggtcaaggac gtcaggatat
     5161 tacccacttt atttgtgaat tggctcaaat tatgcttggc ttggcaaatg ttaacaagac
     5221 tgttgaagaa gttcgccaac atcttgagga tggtcaagca ctggctaagt ttgaggaaat
     5281 ggtgcaagcc caaggtggag acttggaaga cctctatcgt cctgtaaatg tagctcatgt
     5341 ggtggaaatc cctgctcagg aaacgggtgt catttcagct cttccagcta tggactttgg
     5401 tctttatgcc atgaggctgg gtgctggtcg tgcagttaag tctgatgcct tggactatga
     5461 aacaggaatt gtttttgaaa agaagattgg agactccgtt caaaagggtg aaattgttgc
     5521 aaaagtatac acaaatgaaa aaattccacc tcaactagtt acagatttcc aaaaatgtgt
     5581 taaaataagt gatgaagtga aaaaaatccg tgaaattgta gaaatcatct cctaaatgga
     5641 gggaaaaccg aaatgaaatt aaataaatat atagatcata cgcttttaaa acaagatgca
     5701 aagaaaaaac aaattgatag tttgttgtct gaggctagag agtatggctt tgccagtgtt
     5761 tgcgttaatc caacctgggt tgaacatgct aaaaaagggc ttgaaggcac agatgttaag
     5821 gtttgcacgg tagtaggttt ccctttggga gcaacaactt cagccgtgaa agcatttgag
     5881 acaaaagaag ctatccaaaa tggtgcagat gagattgata tggtgatcaa tgttggagct
     5941 ctcaaatcag gtaatttagc cttggttgag tcagatatta gcgcagtagt ggaagcaagt
     6001 ggtgataagt tagtgaaagt cattattgaa gcttgccttc tgacagacca agaaaaagtt
     6061 gttgtttgcc aattggccca aaaagctggg gctgactttg tcaaaacatc tactggcttt
     6121 tcaactggtg gtgctacgat agcagatgtt acattaatgc gtgaaacagt tggatctgat
     6181 atgggagtca aggctgctgg tggagctcgt tcttatgcag atgctcttgc ctttgtcgaa
     6241 gcaggtgcga cccgtatcgg aacgtcagct ggggtagcta ttttaaaagg agaattggca
     6301 gatggcgact actgagttga ttgaactggc aattgaaacc agcaaacatg cctatgtccc
     6361 ctattctcac tttcctatcg gagcagtttt agtagccaaa gacggaaatg tttacacggg
     6421 agtgaatatc gaaaatgcta gctatccttt gaccaattgc ggtgagagaa cagctatttt
     6481 taaggctata tctgaaggcc aaagagaatt ttcagaattg attgtctatg gtcaaactga
     6541 aaaaccgatt tcaccatgtg gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga
     6601 tttaaaagtg accttagtcg caaaagataa atcgacggtc gagatgacgg tcggggagtt
     6661 acttccatat tcttttacag acttaaatta gtctgagtcg ctctttgagt ggcaagggtc
     6721 tttgtgacca atcaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcag gcttggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttt ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaga atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 agcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtcc aagttgacta
     7381 cgctggttca tttggtgatg cggctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgaccg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aactagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagca
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta taggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctaccaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtattgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg agattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaagaac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta tttctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gccctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttggct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactt tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agcttgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctttataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccttggg aatcaacgtc
    11161 tacaagatga gatatttagg gattattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactac
//
LOCUS       DAVMAF010000060        11545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMAF010000060 DAVMAF010000000
VERSION     DAVMAF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.060"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            150..2303
                     /gene="blpA"
                     /locus_tag="VYO97_001594"
     CDS             150..2303
                     /gene="blpA"
                     /locus_tag="VYO97_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HEV1754363.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2314..3675
                     /locus_tag="VYO97_001595"
     CDS             2314..3675
                     /locus_tag="VYO97_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1754364.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            3732..3887
                     /gene="blpC"
                     /locus_tag="VYO97_001596"
     CDS             3732..3887
                     /gene="blpC"
                     /locus_tag="VYO97_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1754365.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(3931..5271)
                     /locus_tag="VYO97_001597"
     CDS             complement(3931..5271)
                     /locus_tag="VYO97_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1754366.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(5285..6022)
                     /locus_tag="VYO97_001598"
     CDS             complement(5285..6022)
                     /locus_tag="VYO97_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1754367.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRFLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6027..6365)
                     /locus_tag="VYO97_001599"
     CDS             complement(6027..6365)
                     /locus_tag="VYO97_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1754368.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6642..6971
                     /locus_tag="VYO97_001600"
     CDS             6642..6971
                     /locus_tag="VYO97_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1754369.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7139..8188)
                     /locus_tag="VYO97_001601"
     CDS             complement(7139..8188)
                     /locus_tag="VYO97_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754370.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LGWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8185..8916)
                     /locus_tag="VYO97_001602"
     CDS             complement(8185..8916)
                     /locus_tag="VYO97_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754371.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            8984..9394
                     /locus_tag="VYO97_001603"
     CDS             8984..9394
                     /locus_tag="VYO97_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1754372.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9404..9691
                     /locus_tag="VYO97_001604"
     CDS             9404..9691
                     /locus_tag="VYO97_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754373.1"
                     /translation="MKLSNLLLFAGAATGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10058..11194)
                     /gene="dnaJ"
                     /locus_tag="VYO97_001605"
     CDS             complement(10058..11194)
                     /gene="dnaJ"
                     /locus_tag="VYO97_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1754374.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGGLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         3732 a         2513 c         2049 g         3251 t
ORIGIN      
        1 tttcaagcca cctctaaatg tttaaaaaaa ataatttcta atcacttttt taccattcag
       61 gaagttttaa tgactattca agatttcata aaatatgaac ttagttttat ggcataatat
      121 acctatccac tatatgaaag gaattgccaa tgacttctta taaacgtaca tttgttcctc
      181 aaatagatgc gagagactgt ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt
      241 cagatttttc tctagctcac ttgagagaac ttgcaaagac caataaagaa gggacgactg
      301 ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga aacaaggcct gttcaagcag
      361 ataaaactct ctttgacatg agtgatgtcc cctatccatt tatcgttcat gttaacaaag
      421 aaggaaaact ccaacattac tatgttgtct atcaaacaaa gaaagactat ctgattattg
      481 gtgatcctga cccttctgta aaaatcacta aaatgtcaaa agaacgcttt ttctctgaat
      541 ggactggagt agctattttt ctagctccca aacccagcta tcaaccccat aaagataaaa
      601 agaatggtct actaagcttc cttcctctga ttttcaaaca aaaatctctc attgcttaca
      661 ttgttctctc aagcttattg gtcactatta tcaatatcgg tggttcttat tatctccaag
      721 gaatcttgga tgaatacatc ccaaatcaga tgaaatcaac tttaggaatc atctcagttg
      781 gtctggttat cacctatatc ctccaacaag tcatgagctt ttccagagat tatctcctaa
      841 ccgttctgag ccagagatta agcattgatg tgattttatc ctatattcgc catatttttg
      901 aacttcccat gtctttcttt gcgacacgtc gtacaggaga aatcatttca cggttcacag
      961 atgctaactc tattatagat gccttggctt ctaccattct ttctcttttt ctggatgttt
     1021 ctattctgat tcttgtaggg ggcgtcttac tggcacaaaa ccctaatctc ttcctccttt
     1081 ctcttctttc cattcctata tacatgttca tcatcttttc ttttatgaaa cctttcgaaa
     1141 aaatgaacca tgatgtcatg caaagtaatt ctatggttag ctctgccatt atcgaagata
     1201 tcaacgggat tgaaactata aagtcgctca cgagtgaaga aaatcgctat caaaatatag
     1261 acagcgaatt tgtagattat ttggaaaaat cctttaagct cagtaaatat tctattttac
     1321 aaacgagttt aaagcaggga acaaaattag ttctgaatat ccttatccta tggtttggcg
     1381 ctcaattagt catgtcgagt aaaatttcta tcggtcagct gattaccttt aacacacttt
     1441 tttcttactt tacaactcct atggaaaata ttatcaacct ccaaaccaaa ctccaatctg
     1501 cgaaggtcgc taataaccgt ttgaacgaag tctatctagt cgaatctgaa tttcaagttc
     1561 aagaaaaccc tgttcattca cattttttga tgggcgatat tgaatttgat gacctttctt
     1621 ataagtatgg ttttggacga gataccttaa cagatattaa tctcacgatt aaacaaggag
     1681 ataaggttag cctagttgga gttagtggtt ctggtaaaac aactttagcc aaaatgattg
     1741 tcaatttctt tgaaccctac aaagggcata tttccatcaa ttatcaggat attaaaaaca
     1801 ttgataaaaa agtcttgcgc cgtcatatta attacctacc ccaacaagcc tatatcttta
     1861 atggctctat cttggaaaat ttaaccttgg gcggtaatca tatgattagc caagaagata
     1921 ttctaagagc ttgtgaatta gctgaaatcc gtcaagacat tgaaagaatg cctatgggct
     1981 atcaaactca gctctctgat ggagctggtc tatcaggagg acaaaagcaa cgaatcgctc
     2041 tcgctcgtgc tcttttaact aaagctcctg ttttaatact agatgaagct actagcggtc
     2101 ttgatgtctt gactgagaaa aaggttatag ataatcttat atccctaact gataaaacca
     2161 ttctctttgt agcccatcgt ctcagtatag ccgaacgaac taaccgtgtc attgttcttg
     2221 accaggggaa aatcattgaa gttggtagtc accaagagtt aatgcaggcg caaggcttct
     2281 accatcatct attcaataaa taaggagaat gtcatgaatc ctaatctttt tagaagtgtc
     2341 gagttttatc agagacgtta ccataactat gcgacagtgt tcattatacc tctttcatta
     2401 ctatttactt tcatcttgat tttctccctt gttgccacaa aagaaattac tgttacttcc
     2461 caaggagaaa tcgcccctac aagtgtcatt gcatctattc agtcgaccag tgataatcct
     2521 atccttgcta atcatttagt ggcaaatcaa gtagttgaga aaggtgactt actcatcaaa
     2581 tactctgaaa caatggaaga aagtcagaaa actgccttag caactcaatt acaaagactt
     2641 gagaagcaaa aagaaggact tggaatcttg aaacaaagct tagaaaaagc gactgatctt
     2701 ttttctagcg aggatgagtt tggctaccat aataccttta tgaattttac taaacaatcc
     2761 catgatattg aactgggtat ctcaaagact aacactgaag tttcaaatca agctaatctt
     2821 accaatagca gttcatcagc catcgaacaa gaaattacaa aagttcaaca acaaattgga
     2881 gaatatcaag agttgagaga tgctatcata aataacagag cacgcttacc aactggcaat
     2941 ccgcaccagt caattttaaa tcgttatctt atagcctctc aaggacaaac acaaggaacg
     3001 gcagaggagc catttttatc tcaaattaat caaagtattg caggtctgga atcatctatc
     3061 gcaagcctca aaattcagca agctggtatc ggaagtgtag caacttatga taatagttta
     3121 gcaaccaaaa ttgaagtact ccgcactcag tttttacaaa cagcctcaca gcaacaacta
     3181 accgtggaga atcaattaac agaattaaaa gtacaactag atcaagcgac acagcgctta
     3241 gaaaataata ccttaaccgc tccaagtaaa ggtatcgttc atctgaacag cgaatttgaa
     3301 ggtaaaaata gaattccaac tggtacagaa attgctcaaa tattccctgt catcacagat
     3361 acaagagaag tactaatcac ttactacgta tcatctgact atctcccctt actagataaa
     3421 ggacaaactg taagattaaa actggagaag attggaaatc acggcaccac catcatcggc
     3481 caacttcaga gtattgatca aactcctacc agaacagagc aaggcaatct ctttaaatta
     3541 accgctcttg caaaactatc taacgaggat agtaaactca tccaatatgg cttacaaggc
     3601 cgcgtcacta gtgtaactac aaagaaaaca tattttgatt atttcaaaga taaaatttta
     3661 acccattctg attaattttc agataacact ctataactat ttattatctt atcaaaaagg
     3721 agaatcataa catggataag aaacaaaacc taacttcatt tcaagaacta acaactaccg
     3781 aactcaacca aattataggt ggaggatggt gggaagattt cttatataga tttaatataa
     3841 ttgaacaaaa aaatacaaaa ggattttatc agccaataca actataaaaa aacaagacca
     3901 agagaacgca ctctcggtct tattttttct tcattctata tgtattacag taagtacctg
     3961 acgaaaaacg tgatcttggc aggtggtatt gagactggta ttgggatgac tttccacaat
     4021 tttcataacg gtatagagac caactcctct ctcctcccct ttagaacttg ctccaaagga
     4081 gaagatttca gaaatatcga ttccctcttc tttgatggaa ttttcaatga taaaggtctc
     4141 ctgtgctcca tttttaaaaa aggcaattga aacatgaggt tgacaggctt ctacactagc
     4201 ttcaatagcg ttgtcacaaa ggatagacac aacggttaga aaatcaagta gactcacccc
     4261 ctctacctga atctcctctg gaacttcgac attaaagaca atgttcttat ctctagcttt
     4321 tataaatttt ccagctagga gacttttgag ggcacgatcc cgaacattca ccaatcggcc
     4381 aaggtcatat ttattgtcct gcaatttttc actagaatcc tttaagaccg agtcgtagat
     4441 ctcttttatc tgctccatat cctcctcttc aatgcccaga cgtaagctag tcaagaggtt
     4501 ggtgtagtca tggcgaaaac tccgtacttc cttataaagc tcctctatat gccgactata
     4561 ccgctccatc tctctgtagc gcaagtcctg ctcttggttc agtctctcat ggagtttgtc
     4621 cttcaaatag gtatccaatt tcttgataat ccccataaaa aagagcaggt aaaagactag
     4681 gatgagatgg cgaacagtcg ttgattgaat cccctgttca tattcaaagt aagacagatt
     4741 ttgtatcacc aaatagtaag cccccattat ccagttaatc tgagtcaggg acttttgaaa
     4801 atctttatcg agaatcccct ttctcaagct agtgaaatca tagtccaacc atttcaaaaa
     4861 agctagagaa atgaagaaat tgaaaattat tatacataac caaataaatg agtagtcatc
     4921 atatacttgc ccttgtccca aaaatggaag cacaaaatag gaaacacctc tataaaagag
     4981 attcactaat atcattggaa agagaccata aaagagaagg agttttttag gaagttctct
     5041 taacaataag aaagataagc ctatgccgta caagggttcc ataaaataag ataggtaaac
     5101 atttcctacc atatagccaa gcattccaaa aacaacggcc aacagtatct taaaaagaaa
     5161 ggccttaaaa atcctctcaa aagtgagact aattccatct accttaaaga atatgacaat
     5221 ttttaatccg tgagtaacaa gtgtatacaa caatatccaa gcaacattca taaactctcc
     5281 tagctcagtg taagttatta atagcctcag acacttccct gaccttataa cgcgcgatta
     5341 gacaacttcc accattggga aagaaaagca gtttttcttt cttatctaaa tgcaccacat
     5401 tggcaggatt gatgagaaaa gagcgatggc actgcaagaa acgaggctcc tgcttgagaa
     5461 cctcctctag actcgctgta aattccagcc tgtctgtctt ggtatagaga ataacacgat
     5521 ggggtcttgt ggacgtttcg agatagtaaa cctctttaaa aggatactgg aattgagcaa
     5581 attttgattt aaagtaaaag caatcttccg ctagactttt actgtcttga ccattggcat
     5641 agaggagggc tgtctcgata cgagattcaa actcctctgc cgaaagggcc ttatcaatat
     5701 aatccagagc agacacttgg tagcgaaagg acaggggcat aaactccgag tgagtcgtca
     5761 caaagacaat cagggcatac ggatcccgat cccgaatctt tctagccact tccagaccct
     5821 tcatctcctc atttcgaatc tcaatatcca aaaagaatag ctgatgggcc cccttctcat
     5881 gcacctctgc cagcaattga tctggcttac caaaaacttc aaaagaactc agagtgatat
     5941 gatgttcctt caagagtttc tcaatcgtcg tttcaattct agtctgttgg gaaaaatcat
     6001 cttctaaaac aaatattctc atcttcttac tctccttctt tcaaccattt ttgacgaatt
     6061 tctctatagc gacgtctggc atacttgaca gcgtattgac cttcttctcc aagaaaaagg
     6121 attctttctt gaaaatcaat cgattttact ttatcaagat tgaccaaaca atttcgatga
     6181 catctcatca aggtttcccc atattggttc tcaagattac ttaaattttg aaccatatta
     6241 aaactagcct cttctgtaac aatctttaca gtatgggctt tagttggatg tgtttggata
     6301 tagtagacat cgtcgacgtt tactttatag actgttttct gtgtctgaat aatcatatgt
     6361 ttcattggat tctcctccta agaaaagaat aacagaaaaa atctaacaca agcacaccca
     6421 atcctaaaca gtctttgaaa gctcctaaaa gaattatttt tctacaatat catgatgagc
     6481 ttaccactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
     6541 aagatttaaa ggcgaaaaag gagataaaag tgatagactg acagtatcaa aacacagttg
     6601 ctagaatcaa gactggcacc cagattagag gaggaattct catgacagat acagacccta
     6661 tcaaaagagc tcagactttg attactgact taaacaaagc ctatcaagca tgcaaacagg
     6721 caaccgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca
     6781 aggctgaaac agtggataat cgattcttga ttgaattgga aaaattttac cagacttcca
     6841 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
     6901 cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat gagaatcaaa
     6961 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaagacga cgctgacgtg
     7021 gtttgaagag attttcgaag agtataagtc tctacggacc aaccattatc ctgtagtaac
     7081 aaaaaagaag ttgagacaga ctgaactcaa cttctttttt agtgtcgtat cagcaatgtt
     7141 agtcctgcat ctgacgtttt acctgataag gcaaatacaa gactagtaaa accaaaccag
     7201 ctcctagtaa ggctagaagg gctagttttt cttggaaggt aatcaaccca acaactactt
     7261 ccacaagtaa aacaaaactg gtcaatcctc gaactacctc ctgtaagcct ttttcctttt
     7321 gccccttgtc cagcggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg
     7381 ccagcaactg gaagagcaag aggtagttaa agagaactac cactgctgtc gcaatccaag
     7441 cttgctcgat aaaaacctgc gccagtacgg aaagtaagag aagacgaaga ctgagagcaa
     7501 agaggtcgcc atttcgcaga taagaacgca gatagagatt ttgccaaatc ttcccaggca
     7561 ccttctgaac agcctttaag ataaagtcca gataggcacg acgcttgaca ctgtttgaaa
     7621 ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa gacttgctta cgcttgcttt
     7681 cttgagaaat aagatagtcc cagcccagtc cagtttcagt gaaaaatttg ctggcctttt
     7741 gacgaaagtg gaaatatttt cctaccccca ataaaagcac atagagcaga aaaactggca
     7801 agccataacc cattgctaaa aacaagggcg caaataacag caagaaaagg gtctgtacaa
     7861 agagccaaaa gactagggaa atgccagttt gacgcttgag atggagcttg atttcctctt
     7921 ctccaactaa gagaaagagc ttgtctggag cctccatata ggtggcaatt cctccccaaa
     7981 gtaaaagtaa aacagacgta attcctacaa acaaaaggat aggccaatga ttttcaggaa
     8041 aatgttgtaa gagttgactg tactggtagg ctagaaagcc caacaggaca agcaggaaca
     8101 agacaaagtg gtcattgagc acatagcgca gataaccaag acactcctta cgaaaggcct
     8161 gctttctctt taaaaacaag tctttcatag atcctcctct ttggtcagag ccaagtaaat
     8221 atcattcaaa ctagcctcag gcatatcaaa ggcttcacgt agttgcagga gattgccttt
     8281 ggcacgcacc tctcccttgt gaagaatgac aaaggcatca cacatcttct ccgccgaatc
     8341 cagcacgtgg gtactcatga gaatagactt gcccttttgc ttctccactt ccaaaagctg
     8401 aatcaaatca gaaatagcca gcggatcaag accaaggaaa ggctcatcca cgatgaaaag
     8461 acttggatcc accacaaaag cacagataat catgaccttc tgcttcatcc cttttgaaaa
     8521 atgaacaggg aaccagtcta atttctgttc caaacggaac atttttaaca agggctctac
     8581 tcgttcgaaa gccacttttt gctcaatacc gtaagccata gcaaccgttt cgatatgctc
     8641 tctgagggtc aattcctcat acagactagg cgtctcagga atgtagccaa tctgcttgcg
     8701 gtagctagtc gcatctcctt gcagagtcag gccattgata ttgatggagc cactataagg
     8761 tgccaacaga ccgataatct cattgatcgt cgttgatttc ccagcaccat tgagaccaat
     8821 caaaccgacc aactgcccac tttcaacagt aaaggacaca tctttcaaaa caggaacatg
     8881 aacatagcca cctgtcaggt ttttaatttc taacatattt tctccaaatc tggtataatg
     8941 tagctatatt atatcaaaat tcagtacagt agaggtagat tttatgtcag attgcatttt
     9001 ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta tatgaagatg agcaggtcct
     9061 tgcctttctt gatatctctc aagtaacact aggacacacc ttggtcgtgc caaaagaaca
     9121 ctatcgcaat cttttggaga tgaatgctac gagcgccagc caactctttg cccaagtacc
     9181 aaaagtagct caaaaagtca tgaaagtcac taaggctgct ggtatgaata tcatttccaa
     9241 ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac gttcaccttg tgcctcgcta
     9301 cagtgctgac gatgacctca agattgattt tatcgcccac gaaccagact ttgacaaact
     9361 tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct
     9421 actgctattt gcaggagctg caaccggaag ttatctggtt acaaaaaatc gccaaaccat
     9481 cacagatgaa gtcttgaata ccactgaccg cgttcaagct atcaaagacg atgtggatat
     9541 tatccaaaat agcctgcaaa tcatcgacca gcaaaaagaa cttatcaagg aataccaaga
     9601 agacttgact tacaagttta aggtcttgga aaaggatatc cagactagac tagctgtgat
     9661 aaaagaaatg cagggaactg aagataagta aaaagagccc cccggctctt ttttaaaacg
     9721 taaacaagtt atagtgaatt gaatttagaa tagcacatca tagtttctaa agcattgtta
     9781 gagattactt taaaatcctc ttatcaattt gttcatattc tatttcaatc tactatatat
     9841 atcccatcaa ctatgcatca taatttaggt aactcatact caataaaaat caaaaagcaa
     9901 actagaaagc taggcacaga ctgctcaaaa caccgtttta aggttgtgga tagaactgac
     9961 gaagtcagct caaaacactg ttttgaggtt gtagatacga ctcacatata tacgacaagg
    10021 caacgctgac gtggtttgaa gaaatttttg aagagtatca ttctccatca aaggcatctt
    10081 taatatggtc aaagaagcct tttttctttg gatttacttt caagccacca gcagccgcga
    10141 attctttcaa ggctactttt tggcggtcgt tcaagcctgt cggtgttacg acattaacag
    10201 taacgtattg gtcaccaact gcaccgccac gaaggctcgg tgccccctta ctacgtaggc
    10261 ggaatttctt accagtctga gttccctctg gaataaccaa ttcaacatca ccgtgaacag
    10321 ttggaatatc tactgtatca ccaagagccg cttggacaaa gttgaggttg agattgtaga
    10381 agatagtcgt tccttcacgt tcaaacttgt cactagcttc cacagaaact actacataca
    10441 agtcaccata aggtccaccg ttaaagcctg cttcaccttg accagcgagg cgaatttgtt
    10501 gacctgtttc cacaccagca gggattttca catgtacgct atgagcttgt ttctcatgac
    10561 ctgttccatg acaggttgta catggatatt tgatttcttt tcctcgaccg tgacagacat
    10621 cacaggttac ttggcgacgc atcataccaa gaggagtctg cgtatcgacg ttaatgacac
    10681 cagcgccatg acagcgtcca caagtgactg gacttgtccc tggcttagca ccagatccat
    10741 tacatgtacg acagccagct tcacgatgat acttaacttc cttctcagtt ccgaagatag
    10801 cttcttcaaa ggtcaaattg acacgatact ggagatcatc tccttggcga ggagcgtttg
    10861 gattgcgcga agaaccgcct ccgccgaaga aacttgagaa aatatcctca aaaccaccga
    10921 agccacctgc cccattgaaa ccgccgaaac caccagctcc accaaaacca ccattggcgc
    10981 ctgcagcacc atactggtca taggcagcac gtttttggtc gtcactcaaa gtctcatagg
    11041 cttcttgaac ttccttgtac ttgtcctcag caccaggctc cttgttgata tctgggtgat
    11101 attttttgga aagcttacga taagcctttt tgatttcgtc tgccgaagcg tttttggata
    11161 cccccagacg atcataaaat tcagtattgt tcataattca agatacaaag ggcgtcaaac
    11221 ggacacagcc atctaggagt tttgacgacg gacgcttacg tcctagaaaa aactatcttt
    11281 ttggcacagt ccgtagcccg tgttcagtta cgaacaccct tattcctttc tattgatgtt
    11341 ctgaatcgaa ccaccattta ggtcgggttc aattcctttc ttttatattt agtaagaaat
    11401 tctgaaaccc gtgttcagtt acgaacactc tttgttcctt tctattgatg agacaaaacc
    11461 caaacaaatg agattccgtc cctttttatc gaaaaacaga ggctgggttg caacctctgc
    11521 aaataagtaa attaacttta ttttg
//
LOCUS       DAVMAF010000061        11399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMAF010000061 DAVMAF010000000
VERSION     DAVMAF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.061"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(2..1852)
                     /locus_tag="VYO97_001606"
     CDS             complement(2..1852)
                     /locus_tag="VYO97_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1754375.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIILLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1894..3051)
                     /locus_tag="VYO97_001607"
     CDS             complement(1894..3051)
                     /locus_tag="VYO97_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1754376.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNML"
     gene            3109..4192
                     /locus_tag="VYO97_001608"
                     /pseudo
     CDS             3109..4192
                     /locus_tag="VYO97_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
     gene            4331..4657
                     /locus_tag="VYO97_001609"
     CDS             4331..4657
                     /locus_tag="VYO97_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1754377.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4644..5237
                     /locus_tag="VYO97_001610"
     CDS             4644..5237
                     /locus_tag="VYO97_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1754378.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            5230..6141
                     /locus_tag="VYO97_001611"
     CDS             5230..6141
                     /locus_tag="VYO97_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1754379.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPIY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6204..7268
                     /locus_tag="VYO97_001612"
     CDS             6204..7268
                     /locus_tag="VYO97_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754380.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7446..8306)
                     /locus_tag="VYO97_001613"
     CDS             complement(7446..8306)
                     /locus_tag="VYO97_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1754381.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(8581..9567)
                     /locus_tag="VYO97_001614"
     CDS             complement(8581..9567)
                     /locus_tag="VYO97_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1754382.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9839..11314)
                     /locus_tag="VYO97_001615"
     CDS             complement(9839..11314)
                     /locus_tag="VYO97_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1754383.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     IWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEVLNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFGTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
BASE COUNT         3343 a         2510 c         2062 g         3484 t
ORIGIN      
        1 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
       61 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      121 ccttaccaac gaaaatctta tccataactt gattgtcaac gagttcggtt gataccaaga
      181 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      241 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      301 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      361 ggctagcttc tggaatggtc ataaagtaac gggtcatgcg gaagtctgtc accgttacag
      421 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      481 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      541 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      601 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
      661 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      721 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      781 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      841 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag ataatgcgtt
      901 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
      961 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1021 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1081 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1141 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1201 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1261 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1321 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     1381 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1441 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1501 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1561 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     1621 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     1681 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1741 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1801 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     1861 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     1921 tattttatcc aaataaatta ttgttccctt gtactccttc ttccatttaa atcctctaat
     1981 taaacctaca atcgtcccaa agccataagc aaagtgaatg gaaaataaaa gaaagggcat
     2041 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta gtgacaaaag
     2101 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2161 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     2221 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2281 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2341 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2401 atttactaaa ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga
     2461 aacatatcta tcctcggaac tatttcgata attggcaata ctacttccaa acatattttc
     2521 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     2581 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaactctc
     2641 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     2701 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2761 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2821 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     2881 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     2941 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     3001 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3061 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     3121 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3181 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3241 ggtgtcttga ccagcctgcc tcttcttctt gaccatcggc tcacttattc gactaatcaa
     3301 tctgcccctg ctctatctag gaaccttgat ggttggggca agtgtcgcag tcatcaatgt
     3361 cctgcttcct agtcttatcc aagccaatca accaaagaaa attggttttc tgaccacctt
     3421 atatgtaacg tctatgggga ttgcaacggc tctggcttcc tatctagctg tgcccattac
     3481 acaagccagt tcttggaaag gacttatcct tctcctcacg ttactctgtc tagcaacttt
     3541 tttggtctgg ctcccaaatc accgctataa tcatagacta gctccacaaa ccaaacaaaa
     3601 aagtcaaata aaggtcatgc gtaataaaca ggtttgggca attattatct tttcaggttt
     3661 tcaatccttg atcttttaca ccgtcatgac ctggttacct accatgtcta tccatgcagg
     3721 tctatccagt cacgaagctg gcttgctgac ttctatctta tctctgatta gcattccttt
     3781 ttcaatgacc atcccaagcc tgacaaccag tttatctact cgcaaccgtc agctcatgct
     3841 cactctggtt tcactagctg gtgtggtcgg catttccatg ctctttttcc caatcaataa
     3901 tttcatttac tggcttgcca tccatctcct catcggaacc gcaaccagtg ccctcttccc
     3961 ttatctcatg gtcaactttt cactcaagac aagcgcccct gaaaagacag cccaattgtc
     4021 cggcctatct caaacaggag gctatatcct agcagccttt gggccaaccc tctttggtta
     4081 cagttttgac ctgttccact cttgggtacc atctgtagct gccctcttgc tcatcgatat
     4141 cctgatgact gtggccctct ttacagtgga cagagcggat aagatccttt aaacttctag
     4201 ttttagctag aagtttttta tatataaaaa ttttacacat ttctcttgac agggctttct
     4261 tttagatgta caatgtatgt gtagaaaaat tatatataaa aatcttacac attagaaaag
     4321 gaggtttccc atgtactttc caacatcctc tgccttgatt gaatttctca tcttggccgt
     4381 actggagcag ggtgattctt atggttatga gattagccaa accattaagc tgatcgctaa
     4441 tatcaaagaa tctacactct atcccattct caaaaaattg gaaggcaata gctttctgac
     4501 aacctattct agagagttcc aaggtcgcat gcgcaaatac tactccttga caaacggtgg
     4561 tatagagcag ctcttgaccc taaaagatga atgggcactc tatacagaca ccatcaatgg
     4621 catcatagaa gggagtatcc gccatgacaa gaactgaata cctgactcag ctagaactct
     4681 atctcaagaa actacctgaa gctgaccgta tcgaagccat ggactatttc agagagctct
     4741 ttgacgatgc tggagtcgaa ggagaagaag aactcatcgc tagtttggga actcccaaag
     4801 aagcggccca cgaagttcta tccaatcttc tcgataaaaa aatcaatgaa gcacccgctc
     4861 aaaaaaataa ccgacaaatt ttacatatcg ccttgttagc cctccttgca gcacctatcg
     4921 gcattcctct gggaatcgcc atcctcgtga ccctgttcgc aatccttgta gccgctttga
     4981 ctgtcattct ggctttcttt gcagtttcca tactgggtat catcggcgga ttcctatttt
     5041 tagttgaaag tttcactgtc ctcgcccaag ccaaatcagc ctttatcttg atttttggtt
     5101 ctggtttact ggctatcggt gcttcttcgc tagttttact aggcatttcc tatgtagctc
     5161 gcttcttcgg tctactcatt gttcgcctgg tacaatttgt tcttaaaaaa agaaagagag
     5221 gtaaccagca tgcgtaaatg gacaaaagga tttctcatct ttggtgtggt gactaccgtt
     5281 atcggcttta tccttctttt tgtaggtatc caatctgacg ggattaaaag cctactttcc
     5341 atgtccaaag aacctatcta tgatagccgt acggaaaagc taacctttgg caaggaagtc
     5401 gaaaacctag aaattactct ccaccaacac gcgctcacca tcacagactc tttcgatgat
     5461 caaatccaca tttcttacca tccatctctt tctgctcacc atgatcttat caccaatcag
     5521 aacgatagaa ctctgagtct cactgataag aaactgtctg aaactccgtt tctctcttct
     5581 ggaattggtg ggattcttca tatcgcaagt agctactcta gtcgttttga agaagttatt
     5641 ctccagctac caaaagggag aactctaaaa gggatcaaca tctcagccaa tcgcggacaa
     5701 accaccatca taaatgctag ccttgaaaat gcgaccctca atacaaacag ctatatcctc
     5761 cgaattgaag gaagtcgtat caaaaacagt aaactcacaa cgcccaatat cgttaatatc
     5821 tttgatacag ttcttacaga tagtcagcta gagtcaacag agaatcactt ccacgctgaa
     5881 aatatccaag tccatggcaa ggttgaactg actgccaaag attatctcag aatcatccta
     5941 gaccagaaag aaagccaacg aattaactgg gacatctcaa gcaactatgg ttctatcttc
     6001 caattcacaa gagaaaagcc tgaatcaaga ggtacggaat taagcaaccc ttacaaaact
     6061 gaaaaaaccg atgtcaagga tcaactcatt gcgagatctg atgataatat tgatctaata
     6121 tccacaccaa gcagacgttg acaaaaacgc ttacatctgc tactatggaa gcatatttat
     6181 ctaactaaaa aggaggttct accatgaaac aagaatggtt tgaaagtaat gattttgtaa
     6241 aaacaacaag caagaacaag cctgaagagc aagctcaaga ggttgcagac aaggctgaag
     6301 aaacgatagc cgatttcgat acaccaattg aaaaaaatac tcagttagag gaggaagtct
     6361 ctcaagctga agtcgaattg gaaagccagc aagaagagaa aattgaagct cctgaagaca
     6421 gtgaagcgag aacaggaata gaagaaaaga aggcatctaa ttctactgaa gaagagccag
     6481 acctttctaa agaaacagaa aaagtcacta tagctgaaga gagccaagaa gctcttcctc
     6541 agcaaaaagc aaccacgaaa gagccacttc ttatcagtaa atctttagaa agtccttata
     6601 tccccgacca agctccaaaa tctagggata aatggaaaga gcaagtgctt gatttttggt
     6661 cttggctagt ggaagcgatc aaatctccta caagtaagtt tgaaacaagt atcacacaca
     6721 gttacacagc ctttctcttg ctcattctgt tttctgcatc ttcctttttc tttagtatct
     6781 atcacatcaa acatgcttac tatggacata tagcaagcat taacagtcgc ttccctgagc
     6841 agctagctcc tttaactctt ttttctatcg tctctatcct agtagcgaca acactcttct
     6901 tcttttcatt cctcttgggt agtttcgttg tgagacgatt tatccaccag gaaaaggact
     6961 ggacgctaga caaggttctc caacaatata gtcaactctt ggcaattcca atcttcctca
     7021 ctgctattgc tagtttcttt gctttctttg atagcctacg atttacagct ctcttgtgtg
     7081 tgattagcat tggaatcatt ctgcttgcca gtctccatat cattacaaga ccaagtcaag
     7141 caagtgaaac cgactccttc tatcaattat tcttgtctgt ccttgtgaac ggagttatta
     7201 tcctcctctt ctttgtagct gaagtggcac tgattggaga ttatcttcgt atcttggcct
     7261 ttctttaaac ttcatactct tcgaaaatca aattcaaacc acgtcagctt cgccttaccg
     7321 tactcaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtagca
     7381 atcctgtcat ccttggcagg attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt
     7441 ctgctttact cttcttgtgc taatgcttta aaatctttgt ctaatatggg ttgcacttct
     7501 atttgattgg cgtctagttg gtggtaaaaa gataggggta atacctctaa gaattttgca
     7561 tagtccaagc gggcaatggc ttcatagtct tctggtttag agccatctcc tgtgatttga
     7621 accaagtcaa tctcccactg cacttcctta tccaccaatt gctcaatata ggctgcagga
     7681 acaaacggtt ctctcggtaa aactgtcttg actccttgag ctagatggta aacgccatgc
     7741 acttttcctt ctccatcctg atagaaggaa actctcgcaa agtaggcatc tgtctcttga
     7801 accgcacgca cacgcgcttc aaactgagcc aagccatctg cctctaaaaa gcttttcaag
     7861 ttctcacgac taaagaaaat aggattaaaa attccttggc aaatcgtaaa acgagctaca
     7921 gggtctgcta gataggcttg aatacttgct tggaaataag cttcaaaatc aaaaccatct
     7981 agcccttcaa tatcagtccc tgtataagca agcaaagcat ctaaaaatga cttgataatc
     8041 tgccagtctg tctttgttag taggtcagga agatcgacac ggtaagcctt ttgaccatca
     8101 gcataactga ccttaaagag aaattgtgat tgccccacta tcgcacactc gatatattcg
     8161 agacgattaa gaggctgacg gagatagact acatcatagc tatgtgactc caaaccgtct
     8221 agcaatgcca agatcgactt ggcattcaaa atctcttgtt ctcctaaaat gctctgttta
     8281 tttggaataa aaaatgtttt cgccataact ggactccttt gaaatcgttt acatacagta
     8341 taccttattt ttctgaaaga tacaaaaaca aactttatac tcttcgaaaa tctcttcaaa
     8401 ccgcgtcagc ttcgccttgc cgtagatata tgtaactgac ttcgtcagtc ttatctacaa
     8461 cctcaaagca gtgctttgag cagcctgcgg ctaacttcct agtttgctct ttgattttca
     8521 ttgagtatta gatttttgag tacaaagcat agaaaaacag cccctgagga ctgtttgatc
     8581 ttatacagta gctgcttgat ccaagctttc accgatagcg gctaggcgct caatcacttc
     8641 agcttgtgtc aattcatttt ttgaaacata gcggttacgt gggtgaacac ggcactcgtg
     8701 tgagcatcca cgaaggtact tgtcttcatt ttcttctgat gtcaagatac gacggttaca
     8761 gaagggattt ccacagttga cataacgttc acatggtgtt ccatcaaacc agtctttccc
     8821 tacgatggtt gggttgacat ggttgacatc aacggcgata cgctcgtcaa atacgtacat
     8881 ttttccatcc caaagctcac cttgaacttc tgggtcttta ccgtaagttg cgattcctcc
     8941 gtgcaattgg ccgacatctt tgtagccttc acggaccatc cagcctgaga atttctcaca
     9001 gcgaacgcca cctgtacagt aaaccacgac acgcttgtcc atgaattttt ccttgttatc
     9061 acggacccat tgtggtaact cacggaagtt gcgaatatct gggcgaatag ctccacggaa
     9121 atgtcctagg tcgtactcat aatcgttacg tgtgtcaagg acaacggtat ctttatcaag
     9181 aagcgcttct ttgaactctt ttggagacaa gtaagcacct gttgtttcaa gtgggttgat
     9241 gtcattgtca aagtcgttgt cttccaaacc aaggtggaca atttctttct tgtagcgaac
     9301 aaacatcttc ttgaaggctt gttcattttc ttcgtcaatc ttgaaccaga gttcttccat
     9361 acctgggagg ctgtgaacgt agtccatgta tttttgagtt gtttcatagt cacctgaaac
     9421 tgttccgtta attccctcgt cagcgactag gatacggcct ttaaggccga ttgatttaca
     9481 gaaagccaag tggtctgcag caaattgctc tgcattttca attggagtat aaaggtagta
     9541 aagtaagaca cgaatatctt ttgccataag atttgttctc ttttctattc ttaaattttc
     9601 taaatttttt atttatccat actagtatac ctgcttgaga aaacgaatgc aatttatttg
     9661 actggaaatt gtagaggatt ctcgaaattt cctgaatggt taaaataagg acaagagaaa
     9721 acagggatat ctatatcctt gtgccaaaaa aaccactgcc ctccccagac caacctgagg
     9781 aaagcagtga ttcttatttt aggagttagg aatgaataca cgaaatcaat ttagctgatt
     9841 attttttgtt tttcaagaat tcatcgtatt gtttttgcat ttcgttcaat actttttcgt
     9901 aggcaccttc agatttcaat ttttccatca attctggaat cgctttatct gggtctacag
     9961 taccagtgtt gatagctgta ccaaattgtt gcattgtgtt agcaatagct gagatttcag
    10021 atttcacatt gtcagtattg aagataaatc caagcgctgg agattcttta gcttctgcca
    10081 attctttctt agaattttcg atttgttggt ctgtaacgtt ttcgttgatg taaaggatcc
    10141 agttgttacc agtgttccat ccacccatgt gagtgtttcc tttgtagcca tcaagaacgc
    10201 gaacacggtt ttctttacct tcaatttttt cccagttctt gccttctgga ccgtaaacaa
    10261 gaccgttcaa gagttctggg ttggtattca agaggttcaa cacttccatt gatttttctt
    10321 tgttcttaga gttgtttgag atgacaaagt tagcaacttg tgttgtttgg tttttcttga
    10381 tgaagttagt aattggtttg atttggatat ctttgttggc aacacgtgaa agcaagctgt
    10441 taccgtagtc agctggtcct actgtttctt cacgaacgaa ccaaatatct tgttgaaggt
    10501 caaatgaagt atcgcttgtt gcgacatctt ttggaatgta gccagcttca tagaatttgt
    10561 gaagagtctt caagtgttct ttgaaacgag gcacttcgta acggtttaca actttagtag
    10621 tatcgccttc aaggtcgata acgaatggaa gaccgtttgc tactgggtag tcaaagttat
    10681 cagatgggat gaaaacttta ccaatagcaa atggtactac gtctggagct ttttctttaa
    10741 tttgtttcaa gactggctca agagtttcgt aagaagtaac acctgaaata tcgataccat
    10801 atttagcaag gagagttccg ttgaaggcaa agttttgaga tgatgcaacg ttggctgcaa
    10861 ctggaacagc gtaaatctta ccatttacag tattaccctt gatgtaagct gggtcaagtg
    10921 ctttgtaaag gtctttacct tcttttttgt acaattctgt caagtcagcg taagcacctt
    10981 tttgagcatt tacaatatag ttatctgcaa aggcaatatc atagttttca ccagatgatg
    11041 tgataactga cattttctta ccatagtcac cccagccaag gtattggata tccaatttgg
    11101 caccaacttt ttcttcaatg attttgttgg catttgctaa caattcatcc aagttgtctg
    11161 gtttgtcacc gatttggtac attttgataa caggtttgtc acctgaatca gcagcttttt
    11221 tgctgttacc tgtcaaattt ccacaagcag caagacctgc agccagagcg actacactag
    11281 cagatgcaaa agcatatttt ttccagtttt tcatgataaa aactcctttt tttattttta
    11341 aacttataaa caatgtaatg atcttatact caatgaaaat caaagagcaa actagaaaa
//
LOCUS       DAVMAF010000062        11031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMAF010000062 DAVMAF010000000
VERSION     DAVMAF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.063"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            7..363
                     /locus_tag="VYO97_001616"
     CDS             7..363
                     /locus_tag="VYO97_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754384.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDNRVGVAPSVLMIVFGGVSLIPFLGWVGGILVII
                     GGSLFLATLKKFKSEE"
     gene            557..847
                     /locus_tag="VYO97_001617"
     CDS             557..847
                     /locus_tag="VYO97_001617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754385.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            898..1590
                     /locus_tag="VYO97_001618"
     CDS             898..1590
                     /locus_tag="VYO97_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1754386.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1643..3076
                     /locus_tag="VYO97_001619"
     CDS             1643..3076
                     /locus_tag="VYO97_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1754387.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMM"
     gene            3140..4054
                     /locus_tag="VYO97_001620"
     CDS             3140..4054
                     /locus_tag="VYO97_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-fucosidase"
                     /protein_id="HEV1754388.1"
                     /translation="MLRETVRFWNAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQS
                     LIWQLFHDFIQAAQELGLDEDLLTEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNE
                     KVEAQHRHASHLVGLYPGNLFSYKGQEYIEAARASLNDRGDGGTGWSKANKINLWARL
                     GDGNRAHKLLAEQLKTSTLPNLWCSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAA
                     LPDAWSTGSVSGLMARGHFEVSMSWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMN
                     QEKIKAKCMGKDCISVATAEGDLVQFYF"
     gene            4304..5005
                     /locus_tag="VYO97_001621"
     CDS             4304..5005
                     /locus_tag="VYO97_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754389.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5007..7716
                     /locus_tag="VYO97_001622"
                     /pseudo
     CDS             5007..7716
                     /locus_tag="VYO97_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(7812..8303)
                     /gene="tpx"
                     /locus_tag="VYO97_001623"
     CDS             complement(7812..8303)
                     /gene="tpx"
                     /locus_tag="VYO97_001623"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1754390.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8426..9355)
                     /gene="psaA"
                     /locus_tag="VYO97_001624"
     CDS             complement(8426..9355)
                     /gene="psaA"
                     /locus_tag="VYO97_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1754391.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9387..10235)
                     /locus_tag="VYO97_001625"
     CDS             complement(9387..10235)
                     /locus_tag="VYO97_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1754392.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10232..10954)
                     /locus_tag="VYO97_001626"
     CDS             complement(10232..10954)
                     /locus_tag="VYO97_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1754393.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3221 a         2056 c         2526 g         3228 t
ORIGIN      
        1 gctttaatta gtggtatcgt cggtcttgtg ggaggaattt tacttcttat tggtcctttt
       61 gtcttgttgg gaacagcggt aaacacagtt gctacaactc ttaatggagg agctactgca
      121 ggggcttttt caggtgttgc cttactcttg aatgccttga agattgcaaa tcttgttctt
      181 ggtatcattg ctattgttta ctataaagga gataatcgtg taggtgtagc tccgtctgta
      241 ctaatgattg tttttggtgg agttagtctc attccattct taggatgggt tggggggatt
      301 cttgttatta tcggaggatc tctattcctt gcaacattga agaaattcaa atcagaagaa
      361 taaaaggtat tttagcatga aaagaacaaa aaagtttatc ggtataggag tagctctatt
      421 atctcttttt cttctagttg catgtggaac ataaagttca aaggatactt caacaagtaa
      481 tgatgagaag acagtagcaa catccaatag ttcaaaagaa acaatcactt tcgatacacc
      541 ggttgtaaca gacgatgtga ttgaatcaat acgcacttat gcagattata tagatcttta
      601 taaaaatatt tttgatgatt attttactaa agctgaggaa ggtttcaaag gcacagctat
      661 ggaaaataat gactcgttta ctaaactaaa agagtcaact caaaaattat tcgatgcgca
      721 gaaaaaaagg ttaaataatg aagatagaat agaaacaact aaaaacaatg tgattgccaa
      781 acattgtcaa acagtccttt cctttttggt tttgactagc ttttttgtga aaaattgtgt
      841 aaaatagaat agataaacga ggggaaacct cggaaaattt aaaggagaat ccatctaatg
      901 gtaaaattgg tttttgctcg ccacggtgag tctgaatgga acaaagctaa ccttttcact
      961 ggttgggctg atgttgattt gtctgaaaaa ggtacacaac aagcgattga cgctggtaaa
     1021 ttgatcaaag aagctggtat caaatttgac caagcttaca cttcagtatt gaaacgtgct
     1081 atcaaaacaa ctaacttggc tcttgaagct tctgaccaat tgtgggttcc agttgaaaaa
     1141 tcatggcgct tgaacgaacg tcactacggt ggtttgactg gtaaaaataa agctgaagct
     1201 gctgaacaat ttggtgatga gcaagttcac atctggcgtc gttcatacga tgtattgcct
     1261 ccaaatatgg accgtgatga tgagcactca gctcacacag accgtcgtta tgcttcactt
     1321 gacgactcag taatcccaga tgctgaaaac ttgaaagtga ctttggaacg tgctcttcca
     1381 ttctgggaag ataaaatcgc tccagctctt aaagatggta aaaacgtatt cgtaggagct
     1441 cacggtaact caatccgtgc ccttgtaaaa cacatcaaag gtttgtcaga tgacgagatc
     1501 atggacgtgg aaatccctaa cttcccacca ttggtattcg aattcgacga aaaattgaac
     1561 gtcgtttctg aatactacct tggaaaataa aaaattgtaa gtctagaatt gatttctagg
     1621 ctttttatgt tagtatggaa gtatgataag gaataaaaaa caagattatg tactggccta
     1681 caagcaacca gtttcaacca cttacatggg ttgggaagaa gaagctttac cgataggcaa
     1741 tggttcttta ggagcaaaag tatttggcct tataggggct gaacggattc aatttaatga
     1801 aaaaagtctc tggtctggag gtccacttcc tgatagttca gattatcagg gtggaaatct
     1861 tcaggatcag tatgtttttt tagctgagat tcggcaggct ttggagaaga gagattacaa
     1921 tctggctaag gaactggctg agcagcacct aattgggcca aaaacgagtc aatatgggac
     1981 ctatctgtct tttggggata ttcacattga gttcagccag caaggtacga ctttgtctca
     2041 ggtgacggac tatcagagac agctgaatat tagtaaggca cttgtgacga cttcttatgt
     2101 ctataaggga acgcgatttg aacgtgaagc ttttgcgagt tttccagatg atctcttggt
     2161 tcagcgtttt actaaggaag gggcggaaac tctagatttt actatagaac tatccttgac
     2221 ctgtgatttg gcttctgatg gaaagtatga gcaggaaaaa tctgattaca aggagtgtaa
     2281 gttggatatt actgattctc atatcttgat gaagggaaga gttaaggata atgatctgcg
     2341 gtttgctagt tatctagctt gggaaacgga tggagatatt agagtttggt cagatagggt
     2401 tcagatatca ggagccagtt atgccaatct cttcttggcc gctaagacgg attttgccca
     2461 aaatcctgct agcaattatc gcaagaaact agatttagag caacaggtga tagacttggt
     2521 ggacacagct aaagaaaagg gctataccca attgaaatca aggcatatcg aggactacca
     2581 agccttattc cagcgtgttc aattggattt ggaagctaat gttgacgcat tcactacaga
     2641 tgatttgtta aaaaattata agccacaaga agggcaggct ttggaggagc tgttcttcca
     2701 gtatggacgg tatttattga ttagttcgtc cagagactgc ccagatgctc taccagctaa
     2761 cctacaggga gtctggaatg cggtcgacaa tcctccttgg aattcggact atcacttaaa
     2821 tgtcaatctg cagatgaatt attggccagc ctatgttacc aatctcctag aggcggtctt
     2881 tccggtcatc aactatgtag atgatttgcg tgtctatggt cgtctagcgg ctgtaaagta
     2941 tgcaggaatc gtctctcaaa aaggtgagga aaatggttgg ttggttcata ctcaagcgac
     3001 tccctttggt tggacggcac ctggttggga ttactattgg ggttggtcac cagctgccaa
     3061 tgcgtggatg atgtaaaccg tttatgaagc ctattcattt tatagggacc aagactatct
     3121 cagggagaaa atttatccca tgttgaggga aacggttcgt ttttggaatg cctttttaca
     3181 taaggatcag caggcgcagc gttgggtgtc ttctccgtct tattccccag aacatgggcc
     3241 gatttcgatt ggcaatactt atgaccaatc tctgatttgg cagttatttc atgattttat
     3301 tcaggctgct caggaattgg gactggatga ggacttgttg actgaggtta aggagaagtc
     3361 tgatttacta aatcctttgc aaatcactca atctggtcga atcagggagt ggtatgagga
     3421 ggaagagcag tattttcaaa atgagaaagt ggaggcccag catcggcacg cttcccatct
     3481 agtgggactc tatcctggca atctctttag ctacaaggga caagagtata ttgaagcggc
     3541 acgtgctagc ctcaatgatc gtggagatgg cggcacaggc tggtccaagg ctaataagat
     3601 caatctctgg gcgcgtttgg gagatggcaa tcgagcccat aaattattgg cagagcagtt
     3661 aaaaacatcc accttgccaa atctttggtg tagccatcct ccttttcaga tagatggtaa
     3721 ttttggtgct actagtggca tggcagaaat gttactccag tctcatgcag cttatctggt
     3781 acctctagct gccctacctg atgcttggtc aacaggttct gtttcaggct taatggcacg
     3841 tggacatttt gaagtgagca tgagctggga agataaaaaa ctcttacagt tgaccatttt
     3901 atcaaggagt ggaggagatt tgcgagtttc ttatccagat attgagaaga gtgtgattaa
     3961 aatgaatcaa gaaaaaataa aagcgaaatg catggggaaa gattgtattt cggtggcaac
     4021 agcagaaggt gatcttgttc aattttattt ttaagaagat gttataaggc agtaatttga
     4081 aacttccttt taataaggat ttaagaatat aggcagtttc caactagttg aaaaagcgtt
     4141 ataatgataa taggaagtaa tactcaatga aaatcaaaga gcacaaacta ggaagctagc
     4201 cgcaggttgc tcaaaacagt gttttgaggt tgtagatgga agctgacgtg gtttgaagag
     4261 agattttcga ggagtataat ttgtttgata gagggtgggt ctgatggctt atattgagat
     4321 gaaacactgt tacaagcgtt atcaggttgg ggacacggag attgtggcca atcgtgatgt
     4381 gaattttgag attgaaaagg gggagctggt tattatcctt ggtgcttcag gtgcaggcaa
     4441 gtcaacagtt cttaaccttc ttgggggaat ggataccaat gatgaagggg aaatctggat
     4501 tgatggtgtt aatattgcgg attatagttc ccaccagcgc accaattacc gtagaaatga
     4561 tgtggggttt gtttttcagt tttataatct agtttctaat ctgacagcta aggaaaatgt
     4621 ggaactggct tctgaaattg tgacagatgc cttgaatcct gatcaggtct tgacagatgt
     4681 aggtctggct catcgtctca ataactttcc agcccagctt tctggagggg agcaacagcg
     4741 agtctccatt gcacgcgcgg tagccaaaaa tcctaaaatt ctcctttgtg atgaaccgac
     4801 tggagccttg gattatcaga cgggcaagca ggttttgaaa attctccaag acatgtctcg
     4861 tcaaaaggga gcgacggtga tcatcgtgac tcataatgga gctttggcgc ccattgctga
     4921 tcgcgtgatt catatgcacg atgccagtgt caaggatgtg gtgctcaacc agcatcctca
     4981 ggatattgac agtttggagt actagcatga tcaagcgaaa aacttattgg aaggacttaa
     5041 ttcagtcctt cacagactcc aaggggcgtt ttttatccat cttgatcctg atgatgttgg
     5101 gatctctagc cttagtaggc ctcaaagtaa ccagtcccaa catggaggcg acagctaatg
     5161 cttatttaac aactgctcaa accttggatt tggcagtcat gtctaactat ggcttggatc
     5221 aagcagacca agaagaacta aaacagacgg agggcgcaga ggtcgagttt ggctatttga
     5281 cagatgtgac tatggataat gggcaggatg ccattcggct gtactccaaa ccagagcgaa
     5341 tttcaacctt tcagctaaga aagggacgac ttcctcagtc agacagggaa atcgctttgg
     5401 ccactcattt gcaaggccaa tacagcgtgg gacaggagat tagttttaaa gaaaaagaag
     5461 agggtcattc ctctttaaaa gaccatactt ataccattac tggttttgtg gattcggctg
     5521 aaatcctctc ccagcgagat atgggctacg caggaagtgg aagtgggact ctgacagcct
     5581 atggggtgat tttacctagt cagtttgatc agaaagtcta caatatagct cgtttgaaat
     5641 atcaagattt agcaggttta aatgcctttt catcagctta tgaagaaaaa tccaagcaac
     5701 atcaagaaga gcttgaacaa attttatcag ataatggcaa ggtacgtctg caacttttga
     5761 aaaaagaagg acaagagtct ctagacaagg ggcaagagac ccttgacaag gctcagacta
     5821 atttgcagga aggcaagcgt cgtttagcag ctgctcaagc tcgtatacag gctcaagaaa
     5881 gtcaactagc cttgtttcct caagttcaga gagagcaggc tagtgctcaa cttacccaag
     5941 ccaagcagga attgggcaag gaagaggaca aactaaagca agctgaacaa aatctagccc
     6001 aagaaaagga aaaattagaa aaacatcagc aagtcttgga cgatttggcg gagccaaggt
     6061 atcaggtcta taatcgtcag accatgccag gtggtcaggg ctatcttatg tatagcaatg
     6121 cttcatccag tattcgagca gtgggcaata tctttcctgt ggtactttat gccgtagcag
     6181 ccatgggtga cctttacgac catgactcgc tttgtagacg aagagcgaac tcatgcaggg
     6241 atttttaagg ccttgggtta ccgtagtaag gatattatcg ccaagtttct cctttatgga
     6301 ctagtagctg ggactgttgg aacggctcta ggtagtatac ttggtcatta tttgctagcc
     6361 agtgtaattt caagtgtcat tacaaaaggc atggtggtgg gagaaaccca gattcagttc
     6421 tattggacct atagcttact agctcttgtc ttgagctggt tggcgagtgt gttaccagcc
     6481 tatctggtgg cttggaggga acttcatgac gaagcagccc agcttctact tcctaaacct
     6541 cctgtcaaag gagctaaaat cttattggag cgtatcggtt ttatctggcg tcgtctcagt
     6601 tttactcata aggtaacagc ccgcaacatc tttcgttata agcagagaat gttgatgaca
     6661 atctttggtg tggcaggttc tgtagctctg ctctttgcag gtttgggaat ccaatcttct
     6721 gtagcaggag ttccgtctaa acagtttcaa caaatccaac agtatcagat gcttgtctct
     6781 gaaaatccta gtgcgaccaa tcaggacaag gtagagctag cagaagtgtt gaaagggcag
     6841 gagatactag cctaccagaa aatctattct aaaacgctag acaaggattt caaaggcaag
     6901 gctggtcttc aaaacattac tcttatgatg atagagaagg aagatttgac tccctttatc
     6961 catcttcaac atcatcagca ggagctgaca ttaaaagatg gcatcgttat tacagctaaa
     7021 ctcgcccagc tggcaggtgt caaggttggg cagactttag aaattgaagg taaggaacta
     7081 aaggtcgctg ctattactga gaactacgtt ggtcacttta tttatatgag tcaggctagc
     7141 tatgagcaac tttacggaca gctaccccaa gccaacactt atctggtctc attaagggat
     7201 accagtgcaa ctagtatcga aagtcaggcg ggcttgctta tgaatcaatc tgcggtgtcc
     7261 agcgttgtcc aaaatgcttc agccattcga ctcttcgact ctatcgctag ctcactcaat
     7321 cagaccatga ccatcttggt catcgtatcg gttctattag ctattgtcat cctttacaat
     7381 ctgaccaata tcaacgtagc tgagagaatc cgtgaactct ccactatcaa ggttctcggt
     7441 tttcataata atgaagtcac cctctacatt taccgtgaga cgattgtgct gtcccttgtg
     7501 ggaatcgtac ttggtctggt agctggtttc tatttacacc aatttttgat tcaaatgatt
     7561 tcgcctgcga ctattctctt ttatccgcag gtaggctggg aagtctatgt aatcccagtg
     7621 gcaacagtaa gcatcatttt gaccttgctt ggtttcttcg tcaattatta tttgagaaag
     7681 gttgatatgt tagaagccct gaaatctgta gagtaaggta gttattttta gctgattgaa
     7741 cttctattta ctaatattca aaaatcctcc gtttcaaaga gcagggaact ctttgtgaca
     7801 gaggattttt tctatagggc tttagcagct gcaattgcgg cttcgaagtt tggctcagaa
     7861 ttgatattat ccacgtattc aacgtagcga atcgtattgt cagtatcgag gacaaagact
     7921 gcgcgtgcta ataggtgcca ttcgttgatc aagagggcat aatcgcgccc gaaagaatgg
     7981 tcaaagtagt ctgaaagcat aatggcattg tcaaggcctt cagcaccgca ccaacgtttt
     8041 tgagcaaaag gtaggtccat tgaaacagtc aatacgaccg tgttgtccag tccagccagt
     8101 tcttcattaa aacgacgtgt ttgagttgag cagatgcctg tatcgataga aggaacgaca
     8161 ctcaagactt ttttcttgcc atcaaaatca gccagagatt ttttagaaag atctgttgta
     8221 gtaagagaaa aatcaagcgc cttgtcgccg acttgtagtt gtttacctgt aaagcttaca
     8281 ggatttccga gaaaagttac cataggatac tccaatcttt tttcttccat tgtatctgaa
     8341 acagtcagaa ttttccaatg atttgaccgg aaatgtgggc atagaaaaaa cgccagctca
     8401 catgagaatg acgtttttca gaggcttatt ttgccaatcc ttcagcaatc ttgtcaaggt
     8461 tgtatttcat catgctgtag tagctgtcgc cttctttacc ttgttctgcg atagaatcag
     8521 taaagatttg tgcgtagatt gggatgtttg tgtcttgaga aacagttttc attggacggt
     8581 catccacact tgattctaca aagagtgatg gaacttttgt ttggcgaagt ttttcaacca
     8641 aggtcttgat ttgttcagga gttccctctt cttcagtatt gatttcccag atgtaggcac
     8701 ttggaacacc ataggcttta gagaagtatt tgaatgctcc ttcgctggtt acaatgagtt
     8761 tcttttcagc agggatctta ttaaatttat ccttactttc tttatcaagt ttgtctaact
     8821 tatcagtata ttctttgaga tttttttcat agaattcttt attgttaggg tctttggcgc
     8881 tcaattgttt ggcgatattt ttagcaaaaa taataccgtt ttcaaggtta agccaagcgt
     8941 gtgggtcttc ttttcctttt tcattttgac cttcaaggta gataacatca acgccgtcgc
     9001 tgactgcgaa gtagtctttg ttttcagttt tcttggcatt ttctaccaat tttgtaaacc
     9061 aagcattgcc acctgtttca aggttgatac cgttatagaa aatcaaatca gcctcagaag
     9121 ttttcttaac gtcttcagga agtggttcgt attcgtgtgg gtcttgccca atcggaacga
     9181 tactatgaag gtcaattttg tcaccagcaa tatttttagt aatatcagcg atgattgagt
     9241 ttgtagcaac aacttttagt ttttgaccag aagttgtatc tttttttccg ctagcacatg
     9301 ctacaagaat gattgcagaa agaaagagaa cgagtaatgt acctaatttt ttcattagat
     9361 cctccaattt attagggctt tgccccttat tttaacaaat gtttattttt cagtttcaaa
     9421 tatcgttgtt tgggagcgat aaagaagcta atgagaaaga aactagcagc tgtaagcacg
     9481 atactagaac ctgccgcaac attaaaacta tagccaataa agagtcccaa aactgaagca
     9541 gtagctccga aggttgagga aaggaaaatc atacttttca gactattagc atacagataa
     9601 gcagttgcag ctggggtaat cagcatggct acaatcagga tagttccgac actttgcatg
     9661 gctgtcacag acacgagagt caggagtacc atgagaaggt agtgatagaa attgacaggc
     9721 attcccatgg ctttagccaa gagttcatca aaggaagtta tcaagagttg cttgaagaaa
     9781 atccaaatta acaagaggat ggctgccccc acacccatag taataaacat atccgtatct
     9841 tggacggcca gaatattacc aaaaaggata tggaaaaggt cagttgaact tttagcgaca
     9901 ccaatcaaga tgataccgag ggctaagaaa gaagaaaagg taatgccgat ggcggtatcg
     9961 cttttgataa tcgagtttcc tttgatgtag gtaatgatga tggcagctag caatccaaag
    10021 acaatggctc cgataaagaa gtcaaggccc aagatgaagg agagggctac acctggtaag
    10081 acagcatgtg aaatggcatc tcccatgagt gacatcccgc gtagaataat gaaacatccc
    10141 acagctccag ctacaatccc gacgacaata gctgttatca aggcattttg taggaaatgg
    10201 aatttttgca atccatcgat aaattctgca atcataggtc acctccattg aaaaagagtt
    10261 gattaccgta agcttctttt agattggttt cggtaaaagt ttcttttgtt ggaccaaagg
    10321 caatcacttc tcgattgaca agtaagactt gatcgaagta gtggggaatc ttgctgaggt
    10381 cgtggtgaac gatgagaacc gtcttcccag cttttttcaa atctctcagc gtattcatga
    10441 tgatttcctc actgacagag tcaatcccag caaagggttc atccaagagg atatagtcgg
    10501 cttcctgcac caaacatctg gcaatcaaga cccgctggaa ttgacctcca gacagttgac
    10561 taatttgacg ttcagcgtag tcagctaggc cgacgatttc aagggcctct tgcactttct
    10621 tccaatgttt agcctttaaa cttcgaaaga gaggaataga gggaaatagt cctaacgaga
    10681 cgcattcctt gaccttgatg ggaaagttgt agtcgatatt gattttttgt tcgacatagg
    10741 caattcggtg taaggatttt ttaacttcct tgtcatcgag aaatgcctga ccttgatgtg
    10801 ggataattcc cagcatacct tttaatagtg ttgatttccc agcgccgttt ggaccaatga
    10861 tgccggtaat tgttggtcca tggagcacta gtgaaatatc cttaagtgcc aacgtttctt
    10921 tgtaggagac actgaggttt tcgatacgta tcataaactt gtattcctcc tgtctcttaa
    10981 tatacattaa aaaaaaaatt aagtcaagtt aatttttgaa aaaattaaaa t
//
LOCUS       DAVMAF010000063        10803 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMAF010000063 DAVMAF010000000
VERSION     DAVMAF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.064"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            195..692
                     /locus_tag="VYO97_001627"
     CDS             195..692
                     /locus_tag="VYO97_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1754394.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            692..2347
                     /gene="dnaX"
                     /locus_tag="VYO97_001628"
     CDS             692..2347
                     /gene="dnaX"
                     /locus_tag="VYO97_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1754395.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2375..2569
                     /locus_tag="VYO97_001629"
     CDS             2375..2569
                     /locus_tag="VYO97_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1754396.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2719..3489
                     /gene="sufC"
                     /locus_tag="VYO97_001630"
     CDS             2719..3489
                     /gene="sufC"
                     /locus_tag="VYO97_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1754397.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3517..4779
                     /gene="sufD"
                     /locus_tag="VYO97_001631"
     CDS             3517..4779
                     /gene="sufD"
                     /locus_tag="VYO97_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1754398.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4790..6016
                     /locus_tag="VYO97_001632"
     CDS             4790..6016
                     /locus_tag="VYO97_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1754399.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6003..6443
                     /locus_tag="VYO97_001633"
     CDS             6003..6443
                     /locus_tag="VYO97_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1754400.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6497..7909
                     /gene="sufB"
                     /locus_tag="VYO97_001634"
     CDS             6497..7909
                     /gene="sufB"
                     /locus_tag="VYO97_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1754401.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8140..9381)
                     /locus_tag="VYO97_001635"
     CDS             complement(8140..9381)
                     /locus_tag="VYO97_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1754402.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9484..10593
                     /locus_tag="VYO97_001636"
     CDS             9484..10593
                     /locus_tag="VYO97_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1754403.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3197 a         1972 c         2347 g         3287 t
ORIGIN      
        1 atatgattct ttctaatgag ttgttttgtt gcttttcatt ataggtcata tgggactttt
       61 tttctacaca aaataggctc cataatatcc ataggggatt tacccactac aaatattata
      121 gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
      181 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
      241 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
      301 ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
      361 aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
      421 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
      481 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
      541 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
      601 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
      661 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
      721 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
      781 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
      841 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
      901 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
      961 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
     1021 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
     1081 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
     1141 ctttattttg gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca
     1201 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
     1261 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca ggcgggcgga
     1321 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
     1381 tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga
     1441 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
     1501 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
     1561 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
     1621 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
     1681 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
     1741 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
     1801 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
     1861 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
     1921 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
     1981 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
     2041 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
     2101 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa
     2161 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
     2221 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
     2281 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
     2341 agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat
     2401 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
     2461 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
     2521 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
     2581 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
     2641 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
     2701 aaaaagaaag gaactatcat gtcagtatta gagattaaag atcttcacgt tgagattgaa
     2761 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
     2821 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
     2881 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
     2941 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
     3001 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaagg tgatgagaag
     3061 atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct caacatgaaa
     3121 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
     3181 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
     3241 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
     3301 ggttttggtg ctatgatcat cactcactac caacgtcttt tgaactatat cacacctgat
     3361 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
     3421 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
     3481 gaattgtaat tccctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
     3541 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
     3601 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
     3661 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
     3721 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
     3781 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
     3841 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
     3901 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa cgtagaaatc
     3961 acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag
     4021 catattatga ttatcgctgg taaaaattct aagattagtt atctggagcg tttagagtca
     4081 cgcggtgaag gaagtgacaa agcaactgcc aatatcacag tggaagtgat tgcacgttct
     4141 ggtgcgcaag tcaagtttgc tgctattgac cgtctaggtg aaaacgtcac tgcctacatt
     4201 agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac
     4261 gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag ccatgctgac
     4321 ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact
     4381 aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca
     4441 actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcccaa
     4501 caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa cccaattctt
     4561 ttgattgatg aaaatgacgt cactgcagga catgcagcct ctattggtca ggtagatcca
     4621 gaagatatgt actacctcat gagtcgtgga ttggataagg caactgcaga gcgtttggtt
     4681 gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa
     4741 atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt
     4801 agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt
     4861 ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag
     4921 ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc
     4981 gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac
     5041 aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc
     5101 tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac accattctaa
     5161 tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct
     5221 taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt
     5281 tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac
     5341 tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat ctacacctca
     5401 tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat
     5461 ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc
     5521 tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatctg ctagttggaa
     5581 ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc
     5641 gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga
     5701 attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc
     5761 tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca
     5821 cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc
     5881 gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt tttatatcta
     5941 taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt
     6001 caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa
     6061 atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac aatccgactt
     6121 gtggagatgt catcaatctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg
     6181 cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg
     6241 ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc
     6301 aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg tcaggtgttg
     6361 ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa
     6421 ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga
     6481 agaaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg accttggtga
     6541 atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa
     6601 cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt
     6661 ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt
     6721 gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg
     6781 ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga
     6841 agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca
     6901 caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact
     6961 caaggaatac ccagacttat ttaatcaata ctttgcgaag ttggtaccgc cgacagataa
     7021 caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa
     7081 aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg
     7141 tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg
     7201 atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc
     7261 tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa
     7321 cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa
     7381 cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg
     7441 tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa
     7501 gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg
     7561 tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt
     7621 ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc
     7681 atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat
     7741 ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga
     7801 aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt
     7861 tgagctgaac cgcttgatta gctatgaaat ggaagggtca gttggataaa atttgatttt
     7921 atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact
     7981 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg aggctagctt
     8041 cctagtttgt tctttgattt tgagtattag atttactcaa aatcaaggat tttgaagatg
     8101 aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa
     8161 gcggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc
     8221 taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc
     8281 ttcataagtc aaatggccaa ccactgttcc agcttcaagt ggtgctggga ttgctttgga
     8341 atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcga ttagatagat
     8401 atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct
     8461 atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata
     8521 atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc
     8581 atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc
     8641 tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat
     8701 accttctaac atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt
     8761 tttggtgatt tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac
     8821 atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc
     8881 tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc
     8941 caagagtttt gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc
     9001 tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac
     9061 agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata
     9121 aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc
     9181 atagaccaga taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc
     9241 atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc
     9301 aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa
     9361 agttaaaaat atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg
     9421 ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa
     9481 ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc
     9541 atcttttacc tatggagaca gatggggtct ttgattaacc cttttgttag cgtgcttaat
     9601 acaattatga ttccattttt attaggaggc tttctttatt atttgacaaa ccctattgtt
     9661 actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt
     9721 actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag
     9781 ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata
     9841 gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta
     9901 aacttatcct atgtggatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg
     9961 agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg
    10021 gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag
    10081 agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat
    10141 attagtggag tttcgattga cgcaatcatt ataggttgtt tggcttatat tggctatagt
    10201 attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt
    10261 ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat
    10321 ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat
    10381 atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt
    10441 ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc
    10501 tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata
    10561 atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt
    10621 actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc
    10681 acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattctgtg
    10741 tcagggtaag ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttatag
    10801 tag
//
LOCUS       DAVMAF010000064        10568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMAF010000064 DAVMAF010000000
VERSION     DAVMAF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.065"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(95..1519)
                     /locus_tag="VYO97_001637"
     CDS             complement(95..1519)
                     /locus_tag="VYO97_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1754404.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VYO97_001638"
     CDS             complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VYO97_001638"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1754405.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VYO97_001639"
     CDS             complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VYO97_001639"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1754406.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6506..7684)
                     /locus_tag="VYO97_001640"
     CDS             complement(6506..7684)
                     /locus_tag="VYO97_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1754407.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLQSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7764..8604)
                     /locus_tag="VYO97_001641"
                     /pseudo
     CDS             complement(7764..8604)
                     /locus_tag="VYO97_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
     gene            complement(8607..10508)
                     /locus_tag="VYO97_001642"
     CDS             complement(8607..10508)
                     /locus_tag="VYO97_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1754408.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3164 a         2339 c         1900 g         3165 t
ORIGIN      
        1 attataatac caaaaagttc gtattttttc tataattatt attattttat agcaaaaaaa
       61 taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa acaatggatt
      121 tgacatttgt catcgcttca atgctatatt taattccttg cactccagct ccagaacctt
      181 tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta atgtggactg
      241 tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt gtaaagactg
      301 atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct acactagcca
      361 cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc atatcttttg
      421 taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt ggtgtaagag
      481 cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg gctgaagcat
      541 tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt aatttagaaa
      601 cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca atgacacgtt
      661 taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt tgcttggcag
      721 catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca agttccaaca
      781 tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt gaacctgtaa
      841 agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct gaaccacgac
      901 ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct ttagccaaca
      961 agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc cctgcaatca
     1021 aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga gcaatcgcta
     1081 gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt gctgcctcaa
     1141 aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca gcataacgaa
     1201 tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt gctacttctt
     1261 tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct gttttatgta
     1321 aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct gcacgcgcag
     1381 cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc aattcttctt
     1441 gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt ccatttacta
     1501 aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat attttaccaa
     1561 attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca caaacttgat
     1621 ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg aggctaatgt
     1681 ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa taaaaaaact
     1741 gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa acaagggtgt
     1801 gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag tctaggtaaa
     1861 agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag gaggtcttgt
     1921 tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg ttctttctat
     1981 tctttcaatt tcaatgaatg gattttttat caatactcat aactgggaat ttgtctgtgt
     2041 aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact aatatcattt
     2101 aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt tcctgcattt
     2161 tcaaattgat agggttctga tttagcattc acaactacca agaggtgttc tttgccgtga
     2221 acttcataga taaggcagcc gctatgttca atcgcagaat gcacaaagac atgatggtaa
     2281 atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg gatgacttga
     2341 cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac ttggttcaca
     2401 ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt caactcacca
     2461 ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc cataaaggcc
     2521 atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat gatttgctct
     2581 gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt gtcatgggct
     2641 tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg acttcctagg
     2701 attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga cttgatagca
     2761 ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa accaatattt
     2821 ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc tgttcccata
     2881 tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct ttgacgaatc
     2941 atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc gtcaatatta
     3001 tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat ttcgtgttca
     3061 ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg attcatacga
     3121 taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa ggtatggtta
     3181 tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac catcaccttc
     3241 aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt ttctggcgcg
     3301 ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc atcgtattct
     3361 ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt cttgatgtaa
     3421 tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc acccaagaaa
     3481 gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc acgaatgtgc
     3541 atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc cgaaccgtgc
     3601 ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga acgtttgcca
     3661 tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg agggaattgg
     3721 acttgatact gataagtctt acctaccaaa tcttcttcaa catccaaact ccagacaccg
     3781 attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc aaaagtcttc
     3841 caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc tgtcgctgta
     3901 ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacctaa ttctccttgg
     3961 taaccccaat gatgatcaaa actagcactg ttaatggcct tatcaaaggc aaaaggattt
     4021 tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat cctatcatcg
     4081 ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat agaatattct
     4141 ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg tgaacttggg
     4201 tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa atcaattcgt
     4261 tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg atggtaatga
     4321 atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt ctatttcact
     4381 aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc taaactctgt
     4441 ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg gataccaagt
     4501 tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag gtcggtcttt
     4561 ttagaaacac tacctgtcac tttggcaccc agactttcga gtttactttt agcttctgag
     4621 cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa ggccgcatcc
     4681 gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa ttctctgagc
     4741 agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat cacgccacct
     4801 agactttcaa tactagccac ttcctctgaa tctgcctgat acagattttc aattgaatgg
     4861 aaatattgaa gtaaaagctg actagccttg cttccgacat gacgaattcc caaaccaaat
     4921 aagagcttct cggcagaatt ttcctttgac gcttggatag cctgatacag tttagcagcg
     4981 gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg ataaatatcc
     5041 gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg accaaggcct
     5101 gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat ttgagcaggg
     5161 caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa gtcagagtta
     5221 caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt ggactctacc
     5281 acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt atcgtctttt
     5341 cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct aacagtcgta
     5401 ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt acggccaact
     5461 gtccagtcaa ctgataagag ttgagcttct ttttcttcag cagggaactt gtaggctact
     5521 gcccactttg gagccttaac ggtaaaacca agttcttctt gacttgctag gtcgttgacc
     5581 ttgattacca ctccatcaat atcgtaaggt agattttccc gttcctgtcc tacttcttgg
     5641 ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt gaccacaaaa
     5701 cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga agggctggct
     5761 tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc caactgacgc
     5821 agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt ttcttggcgc
     5881 gcttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg aactgtgata
     5941 tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt ttctgtgata
     6001 ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc cttttcataa
     6061 gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg atgagccact
     6121 tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc atcctgcaaa
     6181 ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt gccaccaaca
     6241 cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa ctcgaccaac
     6301 tcacggtaaa ggcggtcata ctcactgtct gaaaccgagg gattatcgct ggtatagtac
     6361 tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt cataagacca
     6421 ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca cttagtttga
     6481 aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt cgaacataaa
     6541 taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa gaagagagat
     6601 gtacactttg caagactgtc ataaatcctt ttgcaatagg cataaataga atagctaagg
     6661 taaaaattgt actcagtact ctcccaagaa attcgctctc aaccttggtt tgtacttgag
     6721 taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt ccagaaaagg
     6781 aaagaaaagt tggaagtggt aatcccatca taaaaactcc aacacctgtc aaagccagta
     6841 aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc ccaatgatgg
     6901 aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg taaagctgat
     6961 tcgaaaaagg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg gaagctacca
     7021 gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc ttgatatcta
     7081 caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc tcttttggaa
     7141 ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt agcgtcatat
     7201 ggagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca cctacaacct
     7261 gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc accacttcag
     7321 ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc acaatgttag
     7381 caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata gccagacaaa
     7441 gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa cggtctgaaa
     7501 taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc tgatacattc
     7561 ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt ccataatcat
     7621 agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact gcatagcgat
     7681 tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa ggagcttttt
     7741 attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg ttcctgataa
     7801 taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctc ttgcatctgc
     7861 ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa ataatggaaa
     7921 acattacgct cataaagctt aatacctttg tcaataatct tctctgtata agcctcaaaa
     7981 tagttggcat tataactggt aacaattgag ggccaaaatc aacactaatc tcttatggcg
     8041 actaatctct tggtaaaatt cctccctctc cataacttct ctaccaatcc gagtgacata
     8101 gtctacatcg tagaaactat agaggttacc gaaaagaatc aactcataca tggtccattc
     8161 ttctgttttg aagagataat ctgctacctt atccaaatca tcctgcttca tatcataact
     8221 cgcatctctt tgacaaatca gaccttgtag caaaatccag ttcagctcaa aataaagggg
     8281 agtcgtcgaa ctcttagact tttcaagttg ttctctttga agcttttgaa aacctgcaat
     8341 atcgtttgaa tagtaaagtg ggataatctg tgccatcata gacacatgtt catgattatg
     8401 aaaattcctt gccttatcca tgaaattttc gattgttaca tgaatgttat ccaaaatctc
     8461 aaagaaacgg gagactgcca ggtcagactc cccaagctca aagcgagata actgagaggt
     8521 agagcaggat tcgcctgctg cttcctttaa agaataattt ccacttgttc gaaattcacg
     8581 aaatactttt ccaagatgtt ccatctttac acctgatctg ataattcttc ccactcaagc
     8641 atagcttctt cctgacgatg gctgattttg tccagctcag cctgtaattc catgagtttg
     8701 tcggcatcgt ttgtttccaa catttgttca gaaatggctt ggctttgact ttctagctct
     8761 tcaatttcag cttctagact ttcgatttgt cgcatgagtt tgcgaacttc tttttgactt
     8821 tctttctggg cctgatagtc attgactgga cttgcttcct ttgcttgatt gctagttgaa
     8881 gcttcctcag tctgactcat ttctgctgtt gctttcttct caacatagta gtcgtaatct
     8941 ccaaggtaga gagttgaacc attctcagac aattccaaaa catgagttgc cacacgattg
     9001 ataaagtaac gatcatgact gacaaaaagc aaggtaccat caaagtcaat caaagcattt
     9061 tccaacactt ccttgctatc aatatccaag tggttggtcg gctcgtcaag gatcaggaag
     9121 ttgttgtttt ccatagacaa tttagctaaa agcaaacgag ctttttcgcc accagatagc
     9181 atgccgactg attttttaac atcatctcct gagaaaagga aggctccaag acggttgcgg
     9241 atttcaactt ctggtgtcag tttgaaatca ttccagagtt catccagcac cgtattactt
     9301 ggtgtcagct tgctttgggt ttggtcatag taaccaacct caacattagc gccaaagcgc
     9361 ttttcacctt tgataaaagg aatctggtcc acaatagact tgataaaggt tgacttgccg
     9421 ataccatttg gaccaacgat agcgacagca ttcatcttac gaagatctag gttgataggt
     9481 tgtgacaaga cttccccgtc ataaccaata gcagtatttt caacagtcaa aacaacattg
     9541 cccgacgttt tttcagactg gaaggtcatg ttggctgctt tcttgccagc ttcaggcttg
     9601 tccaaacgct ccattttttc tagttgttta cggcgagatt gagcacgttt ggtcgttgaa
     9661 gcacgaacta gattgcgatt gacaaagtct tccagagcag cgatttcctt ctgttgcttt
     9721 tcatagtttt ttgcctcagt aactagcttt tgctccttca attcgacaaa acgagagtaa
     9781 ttccccacat agcgatccaa ggaatgcttg gtcaaatcta gcgtaattgt cgcaaccttg
     9841 tccaagaaat aacggtcgtg gctgacgata atgagggcac cgctatagtt taccaagtaa
     9901 ttctctagcc aggcgatggt ttcaatatcc aagtggttag ttggctcgtc caagaccaag
     9961 agattgggct tttcaaggag cattttggca agtgccaaac gagtattttg accaccagaa
    10021 agctcagcaa ttttcatctg ccacatagac tcgtcaaact tgaatccatt caaaatcgct
    10081 cgaatatcag cttcataggt aaagccacct gcttggcgaa aattctcaga taagcggtca
    10141 taatctgaca tcagtttatc caaatcctca ccagactttt cacccatctc cagctccatc
    10201 tgacgcagtt gtctctccgt ccgacgcaaa tcattaaaga catgaagcat ttcatcgtag
    10261 atggtatttt cagactcaaa acggctatct tgggctaggt aagacagaga aatatctttt
    10321 ttcttattga tttctccgct agttggctcc tcttctccaa ctaaaatctt caaaagagta
    10381 gacttacctg caccattttt cccaacaagg gcaatccgat ctcgttcatc aacctgcagg
    10441 ttgatattat cgaaaagaac ctctcctgca aaagaacgtt caattttatt agcttgtaaa
    10501 ataatcataa gagtagtata gcatgtttcc ctaaggcatt caagataatc gtaagtcttt
    10561 tagtacaa
//
LOCUS       DAVMAF010000065        10340 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMAF010000065 DAVMAF010000000
VERSION     DAVMAF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.066"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..450)
                     /locus_tag="VYO97_001643"
     CDS             complement(<1..450)
                     /locus_tag="VYO97_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754409.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNA"
     gene            complement(450..1214)
                     /locus_tag="VYO97_001644"
     CDS             complement(450..1214)
                     /locus_tag="VYO97_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754410.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1310..1713)
                     /locus_tag="VYO97_001645"
                     /pseudo
     CDS             complement(1310..1713)
                     /locus_tag="VYO97_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1893..2525)
                     /locus_tag="VYO97_001646"
     CDS             complement(1893..2525)
                     /locus_tag="VYO97_001646"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1754411.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2559..3260)
                     /gene="pyrF"
                     /locus_tag="VYO97_001647"
     CDS             complement(2559..3260)
                     /gene="pyrF"
                     /locus_tag="VYO97_001647"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1754412.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSVMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3493..4713)
                     /gene="divIB"
                     /locus_tag="VYO97_001648"
     CDS             complement(3493..4713)
                     /gene="divIB"
                     /locus_tag="VYO97_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1754413.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTNQRSSRR"
     gene            complement(4723..5781)
                     /locus_tag="VYO97_001649"
     CDS             complement(4723..5781)
                     /locus_tag="VYO97_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1754414.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5785..7137)
                     /locus_tag="VYO97_001650"
     CDS             complement(5785..7137)
                     /locus_tag="VYO97_001650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1754415.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDIPGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(7241..7882)
                     /locus_tag="VYO97_001651"
     CDS             complement(7241..7882)
                     /locus_tag="VYO97_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754416.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKSSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7884..9905)
                     /locus_tag="VYO97_001652"
     CDS             complement(7884..9905)
                     /locus_tag="VYO97_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1754417.1"
                     /translation="MKKLFILLSTFFLSFFFAWIIVLRAPQYLYASYDSVTLLRVKKE
                     AQEPTREVFEQELEKFVNSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKQFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKAIADRKTPPYSLAFRMLLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWHDRVDYYQISFGL
                     GDRVNDTENQNKWYEFSKEAVEKEQALFVKENLIHFANPQGKNENGETLDTYSPDANV
                     LYVSPSYLDKENVAVNDETRQKLAHLQKGEFGLLLPESLRSQEAELKKVFEERLNYYG
                     KSSEDKDAPLEYEMRAIVSYLPTGQKRFVYNNGESPVSIQYLTDPILVVFTPTSTGDS
                     FISKYVWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILSSRDLVIGLLTLFVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(9976..10275)
                     /locus_tag="VYO97_001653"
     CDS             complement(9976..10275)
                     /locus_tag="VYO97_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1754418.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSVVSKWDSHSDKGSAPAGKTSEAWIWTKFGEQVSFYYDYD"
BASE COUNT         3160 a         2241 c         1808 g         3131 t
ORIGIN      
        1 agcattgttt gcaatactta tgccataaaa ttctcttaaa aaatcaacct cctttatctc
       61 caaagagaag ctaaaaccat tactaaatgc aattagaaaa atcaataaaa ataaaatcgc
      121 cgtccaaatc cccgtactaa gagctgctaa tttgaaacta aaactggtaa agtgcttaat
      181 tgatttcaga cgaatgcgac actccaacct attaaaatag ttattcatca aataaaaaaa
      241 gaacaatata tatgtgaacg gaaagcaata tactccagtc gtcatatctt gaagtaaaac
      301 taagatccat tctaatacat ttggatggat tgaatattgg cgacagcgca ataaatatac
      361 tgtactagat aaaacacagg atagcagtaa tataaaataa accaatactg ctaagaaatc
      421 ttttttataa aattgaacaa attgtttcat tatacatagt cctctgaatg tagaaaaaat
      481 gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc actaactaag
      541 gaaagactga tatctttctg atatcccaaa gctagcgttg tcacaggttc taagtaagat
      601 agccctaaaa tagcccataa aataccacca accatcatat aggcaactgg gatgaaaata
      661 gctcctaatt ttttcttcac tagcaaagca ctagctagtc caaaaataga gaacacagcg
      721 ccccaaactc cataccagag agtcgtcaca agactataga gaaactgatt agaatcaaat
      781 aattctttta aggcaccact ataatctcca atataaattt cctgataagg agtcactaaa
      841 agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga agaaataatt
      901 gcagtactac ctacaatagc tagatacctc tttttagaaa ttcggcacaa ttgtgctgtt
      961 agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt acagataagt
     1021 gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat
     1081 aaaccttcaa ttttataaat accattgaaa gcaagaaaat ttcctaaact catgcaaaga
     1141 agggtcaaaa ataaagacat ataaaaccga ggtgattgaa cgactcgtac aagattacaa
     1201 atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca ttcttttttc
     1261 atgctacacc cacaatcaac catctttaag gcttactctg acaagtaagt taataagaat
     1321 ctggactcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt ggcgccatag
     1381 ctaatctagt accagaacca atataattgt caccacctcc attatagtac atgacaatcc
     1441 tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag gcgctacgaa
     1501 tagaggtatt taacctttta ccgccaccag tgctgtcact attattaatt ccagcagagg
     1561 cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac gttaaatcat
     1621 aatttccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca attgtaacta
     1681 cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag aagtgataaa
     1741 caagatagtt gattgatcaa tttgatacat attacatttt cattatatca ataaaagaaa
     1801 gcgttgtcaa ttattttatt taaaatattg tcatatttta aataaaatgg taaaagagac
     1861 tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt ttcttggtct
     1921 tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc ttcttgggct
     1981 agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc atctgcaaag
     2041 ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag tacatctgct
     2101 ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga aataaggtct
     2161 tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt accagctccg
     2221 tggtcttttg gtttgctacg gatgtaggca aaaggcaagt ccatcttatc agcaataatg
     2281 gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc aggaaaggct
     2341 tctttgatag cttccacaaa accattttca attagggtac gagtttctgg ataggctagt
     2401 gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt gaagggttcc
     2461 tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc tttagcaagt
     2521 gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc ctgtgtccat
     2581 tcatccttga tggcatgata agctgcaaca ggatcctcag cttgggtaat gggacgtccc
     2641 actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac tcgtttttga
     2701 tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa atctggattg
     2761 gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc caagccagct
     2821 tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg gatattttga
     2881 aactcctgca tctgagcttc tgacgttgat gtgagctgag ttacagcgat caatttagct
     2941 tgacttccaa gaccttcacg cgcagccttc atcatctcta caccaccagc cgcatgaaca
     3001 ttggtcatat ccacaccaag ctgagatagg atcttcatga ctgacttgac tgtattagga
     3061 atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa gtaggacaca
     3121 atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag gctttcttct
     3181 gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaac taggaaaatc aagagcaatg
     3241 attggacgat gttctcgcat aggtttctcc ttttaccttt actagtgaga gagtctggcc
     3301 aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa atgggtagtc
     3361 tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag gttttctatt
     3421 attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag aactataaga
     3481 gaaaaggtaa acctagcgac gcgatgaacg ctgatttgtt tgatttcgat tgctctcttc
     3541 ctcttgtttt ttctgttctt cttctaaacg tttctgttct tcttcctgtt ttttctcggc
     3601 ttccttggcc tcttgtttgg ctttttcctc agcctccata attaatttat ccgccacagt
     3661 gtaactgtaa attccagctt ccatgtcgac cacactcggt tctgacaatt gtggcttaat
     3721 cttactataa tatggcagtt tcttactcat ttcagataga ggaaccaaga cttcgtccga
     3781 atcattcatg gtcaatcgaa ttaaatcgga tgtcaccttg cttggggcta attccacctt
     3841 ttggatagcc gccttgagtt ctgggctaat ttgagcaagt tctgagacaa aaaccttgat
     3901 ttgttcacta tcattaaaga gaactgataa ataagtttct ggtaaactgt tcagactcac
     3961 agaactagtc tcaagctgac cactggaaag aataggataa tgattttcac cagaaatata
     4021 gtaggccaca atatcatatt ccttgacctt aatagtgaac ttagttggaa attgatagac
     4081 aagttgagct gattcaaccc aatagttaga cttaatctgc ttttcatatt ttgccttgtc
     4141 tagcagaagg ttaatcgtat aatccgaatc ctgaatgcct gaagcctgtc gaatatcatc
     4201 agctgtagtt tgcaccgttc cctcaacacg aatatctttc atggtcgcat aaggactgag
     4261 caagtaggca gagacaatca ataaaagcag acttggaaat aaaatcgtga atgctcgcaa
     4321 gatatggata ccaggaatct ttgctttggc tggtttttcc tttgtagcct ttttagaaag
     4381 ctttttatcc tgttcctcct tctctttaga ctctggttct tctttctctt ctttctcttt
     4441 gtcagcctct gaggatgcta ctttttcttc agactcttcc ttagctgatt ctgaatcttc
     4501 ctggtctgtt tcactctcct ggtcctgttt gtcctctgac ttctcagatt cttctcccat
     4561 tcgagcttgt ctttcctttt ccttctcctc agctagagcc gcctcttctt cagccttctt
     4621 ttttagatat tcttggtttc gtttctgcca ttctgataac tctttcaatt cttcgaggat
     4681 ttctttgtcc tcatttttct tatcttttga catttacttt ccttatgata aatctttttt
     4741 caacaattga taaaaatctg ctagagattt caattcctta gaagccttca tcttagcttg
     4801 gtaatcttcc ttgtgactta gtaagtgaga aagcttctct tccaaactat ccaaggtcaa
     4861 atcgctttct tgaaggtctt ctgcatagcc tttttttaca aagtaagctg cattttcaag
     4921 ctggtcacca cgactagctt cacgaccaag cggcacaatg acatgcaatt ttgctatcgc
     4981 caagagctca aaaatcgtat tggcaccacc tcgtgtcaca acaatatcag ccaattccat
     5041 caagggttga tagagatcgg tcacatagtc aacacgaaaa agattttggc tcaactcatt
     5101 caggctagaa tctccagtta gattgataat attgtagcgc tctgttagtt ctttcttatg
     5161 gtctgtcacc aattggttaa agacacgagc acctgcagaa ccgccaacaa acaatacagt
     5221 cggcaattta tgattaaagt ggctttgaat atccaccaat tcatctggtt ctggattttt
     5281 ttgatctgaa accttggtca ctgctcccac atgctcaacc ttagacaaac tcgaagcttg
     5341 ctcaaaggtt gaatacatct tagtcgcaaa tttataggcg attttattgg ccaagcccat
     5401 agacaggtca gattcgtgaa taaagacagg cactcctgac acacgcgcag cgataacagg
     5461 cggtactgag acaaagcccc cctttgaaaa aagggtctgt ggacgaagtc gcaacatgat
     5521 aaagagcgat tggacgattc cccagcaaac tttgaagacg tccagcatat tttgccaaga
     5581 gaaatagcga cgcaattttc cagtcgcaat ggagtggaaa gtgacatcca aacctgactt
     5641 aaggatttct tggtgttcga taccacgctt gtccccgata tagtggactt cccaaccatc
     5701 ttcgatgaac ttgggcatta acaaaagatt gagggtaacg tgtccaaccg tccccccacc
     5761 tgtaaagaca atttttttca tattttattc ttttaactcc gctactgtgt cgataaagag
     5821 gtcgccacgt acttcaaagt tagcatacat atcccagctg gcattggcag gactaagaag
     5881 aaccacatct ccttgagtcg caagctcata ggccttgcgg gtcgcatctg caatatctgt
     5941 cgcctccaca taagcgacac cagccttgtc tgctgcccgt ttgacacgtt ctgcagattg
     6001 acccaggatg accatcttct tgagtccagg aatgtctggc accaattcgt caaactcatt
     6061 gccacggtcc aaaccacctg caatcaagac gaccttgctg ttgtcaaatc ctgacaaggc
     6121 tttttgagta gccaagatat tagttgattt actgtcgtta tagaatttaa cacccttgat
     6181 gtcatccaca aactggagac ggtgtttgac accaccgaag gctgaaagag tttccttgat
     6241 ggtttgattg tccacaccac gaagcttggc tacagcaata gtcgcaaggg cattttccac
     6301 attgtggcta cctggaacac cgatttcatt cgctgccatg actacttcac cacggaagta
     6361 gagttgacca tcttccagat aagctccatc aaccttttca agtgttgaaa atggtacaac
     6421 agtggcttct gtcttggaag tcaagtcttt tgccaagtct tgattaaagt tcaagacaag
     6481 gaaatcagct gctgtcatct tgttctggat attccacttg gctgctacat attccgaaaa
     6541 tgacccatgg tagtcgatat gagttggcat gaggttggta ataaccgcaa tctctggatg
     6601 aaattcttga acacccatga gttggaaaga agaaagttcc ataacaagcg tgtccttgtc
     6661 cgacgcagtt tgggccactt gactagctgg atagccgata ttccctgata aaagaccatg
     6721 ttgcccagca gcagtcaaaa cttccccaat catagtcgtt gtggttgtct taccgttcga
     6781 tcctgtgata ccaataatcg gtgcttctga aatcaaataa gccaattcca cctcagtcaa
     6841 gactggaatt cccttggcca aagccttttc aatcatggga ttgttgtagg ggatacctgg
     6901 atttttcacc ataagggcaa actcttcatc caagagttcc aaaggatggc cacctgtaat
     6961 gaccttgatc ccttcttcca gcaaactttg ggcagctgga ttgtcctcga aaggtttccc
     7021 atcatttact gtcacaatgg cacctagctt gtccaacaaa cgagctgcag attcaccaga
     7081 cttggccaaa cctaaaacaa ggactttctt atttttaaat tgatctatta ctttcatgtc
     7141 tcgaactcca tttctactcc tactatttta ccatttttat ggaaataaaa aagccacaaa
     7201 gtgtgtttgt gactctttct tctaactgaa tcttaccata tcatctatgt gataaatcgg
     7261 taactcgaat gacctgatcc acttgctccc aaatcagagg attgtgggtc gcaataataa
     7321 tggtccgatt cggatttttt aaagattcta ggatggaaag taattcctca gagtttttgg
     7381 ggtctaagga agcggttggt tcatctgcaa gaatcaaagg cggatccttt aaaattatct
     7441 tcgctaatgc aacacgttgt gcttctcctc ctgataactc aaatataggt tgcttcaaat
     7501 ccaaataaga gaggtttaca cggtttagag cttgtttcat caaagagatt ttctcttttt
     7561 ccttcaactt tttaccaact aaacccagat tgagattctc tttgacggtt tggctttcaa
     7621 ttaagccaaa atcttgaaat aagtatccca agtaatctct aaagaaaaca gaagacttga
     7681 tgtccttaag agaagtgcca tcatagatga tttgcccttt gtcatatggc tctaatcgtc
     7741 caatcatatt caagagtgtt gtcttaccac agccacttgt accgattaag gcataaattt
     7801 tcccaccttc aaaatgaaga tttgtatctg aaaatagctg acggcttcca aattttttag
     7861 atatattctt tagttcaatc atcctatttt cctttcataa ttgtcataga aacacgagat
     7921 tctttctgtg cttgacggta aagcgtcaga actgcactag ctagaaagac gaataaagtg
     7981 agcaagccaa tcaccaagtc tcgactgctt aaaataaaga aactagcacc aaatacaaaa
     8041 ctggcaaatt ggctaaccat atactgagca tgtgtttcaa aaaatcgtaa acctgaaatt
     8101 cgtttaatca agatatctcg gcggaattgc tcgaaatata gaagattgac agaataaaag
     8161 agtaataagg aactggctat cccaacaata gctcctaaga ttaaagttgc tgtttcagtt
     8221 tgaacttcat tataacgagt tagataaaca cttcttcctt ctttaagata ggatacttgc
     8281 tcataaattc cagctttctt taagagttct agcccactct catatccttt gataaagagt
     8341 tgttttccag cattgataga ccaaacatat tttgaaatga aactatcacc tgtagatgtt
     8401 ggagtaaata caactaaaat cggatcggtt aagtactgaa tagatactgg actttcaccg
     8461 ttattataaa caaagcgttt ttgtcctgtt ggaagatagc taacaatcgc tctcatttca
     8521 tactctaaag gagcgtcttt atcctcgctt gattttccgt aatagttcaa acgttcttca
     8581 aaaactttct taagttctgc ttcctgtgaa cgaagagatt ctggtaataa gagtccaaat
     8641 tccccttttt gaagatgagc tagtttctgt ctagtctcat catttacagc cacattttcc
     8701 ttgtctaaat aacttggact cacatagaga acattagcat ctggactata ggtatccagt
     8761 gtttccccgt tttcattttt tccttgagga tttgcaaaat gaatgagatt ctcttttaca
     8821 aatagagcct gttctttttc aactgcttct ttggaaaatt cataccactt attctgattt
     8881 tctgtatcat ttactctgtc acctaagcca aaagaaattt gataatagtc tactctgtca
     8941 tgccatgctt gttttgaaat ttcaagttct ttcaatcgtt ggtaagacgt caaacctgtc
     9001 ttaacagcgt atcccactgt aaagacagca accaactgac acaatagggt taaaatcatc
     9061 aggcgtttaa ggggtaatct tcccttaata acgggaacta atgctttgta actcaaactc
     9121 attagataaa ggagcattag taaaattgaa atcgccaata aaaacaacag atagaaacta
     9181 atcccaaaac cataggtggc taacaagata ggataaaaca aaccttgact aaaaagaacg
     9241 actcccccac ctaggaagga aaggagggct gatagaagga gccacttaat atcagtagat
     9301 aaagaatgcc ccataatgga taagagagtc tgaccagaaa agagttttat acctgctgct
     9361 ctcatttcct taatccgagt gataatcact aaagcaaaga aagataagcc aaatattgct
     9421 aaactaatta aaataagggg atttagtaac attcgaaaag caagagaata gggaggtgtc
     9481 tttcggtcag caattgcttt ataacctaaa tctcctaatt tatcagctag cttttctttt
     9541 gtcaaggagc ctgataaaag gagataacta tttagcggat cactacgttc acgactttct
     9601 tggctagctt cttggaattg ttttggtaat gttccctgac cataagttgc ataagtaaag
     9661 tgagtcgtcc catccttact cggttctaca attctcctag ctattaaact ctgttctgag
     9721 tttacaaact tctccaattc ctgttcaaat acctcacgag tcggttcctg agcctctttt
     9781 ttgacacgaa gtaaagtaac ggaatcatag cttgcatata aatattgtgg cgcacgtaag
     9841 acaataatcc aagcaaagaa gaagctgaga aaaaaagttg atagtaatat gaatagtttc
     9901 ttcatagtag actccttgta aacaaaattc cccctgtaat ttcttacaag gggaattatc
     9961 taagaaaact aaatattagt catagtcata gtaaaatgat acttgttccc caaatttagt
    10021 ccaaatccaa gcctctgaag ttttccctgc aggtgctgat cctttatctg aatgactatc
    10081 ccatttactt acaacagaag aataatgata ttttttacca tgatagtaat ttgaaaaagc
    10141 ccaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat attgtacagc
    10201 agctgatgca gctcccaata atgtaaagct tgaaataaga gctagagcaa gtaatctatg
    10261 ttttttcgtt ttcattttat ttttcctttc aaaaaaagca caccctgagc aacaatgcaa
    10321 caaaataaat cctcctctct
//
LOCUS       DAVMAF010000066        10325 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMAF010000066 DAVMAF010000000
VERSION     DAVMAF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.067"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(14..631)
                     /locus_tag="VYO97_001654"
     CDS             complement(14..631)
                     /locus_tag="VYO97_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1754419.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLCLKSRRK"
     gene            complement(621..1394)
                     /locus_tag="VYO97_001655"
     CDS             complement(621..1394)
                     /locus_tag="VYO97_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1754420.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(1405..2142)
                     /locus_tag="VYO97_001656"
     CDS             complement(1405..2142)
                     /locus_tag="VYO97_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1754421.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVFRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(2147..3277)
                     /locus_tag="VYO97_001657"
     CDS             complement(2147..3277)
                     /locus_tag="VYO97_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1754422.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(3361..3750)
                     /locus_tag="VYO97_001658"
     CDS             complement(3361..3750)
                     /locus_tag="VYO97_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1754423.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(3766..4149)
                     /locus_tag="VYO97_001659"
     CDS             complement(3766..4149)
                     /locus_tag="VYO97_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1754424.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(4150..4938)
                     /locus_tag="VYO97_001660"
     CDS             complement(4150..4938)
                     /locus_tag="VYO97_001660"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1754425.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYHQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(4931..5866)
                     /locus_tag="VYO97_001661"
     CDS             complement(4931..5866)
                     /locus_tag="VYO97_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1754426.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(5996..6172)
                     /locus_tag="VYO97_001662"
     CDS             complement(5996..6172)
                     /locus_tag="VYO97_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1754427.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(6312..7019)
                     /locus_tag="VYO97_001663"
     CDS             complement(6312..7019)
                     /locus_tag="VYO97_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1754428.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            7170..8198
                     /gene="queA"
                     /locus_tag="VYO97_001664"
     CDS             7170..8198
                     /gene="queA"
                     /locus_tag="VYO97_001664"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1754429.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(8859..9986)
                     /locus_tag="VYO97_001665"
     CDS             complement(8859..9986)
                     /locus_tag="VYO97_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1754430.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKYRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVIVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         3045 a         2347 c         1841 g         3092 t
ORIGIN      
        1 aacatctttc ctttcacttc ctacgacttt tcagacacag atagccaaag aagttttcat
       61 agagggcaaa aaagaggagg aaggcatgaa gaaagaaggt ctctggcaaa atcataataa
      121 caggatcctt ggctggatca aaaagccagg tatcatctcc cacaaagaga atttgatgga
      181 aaagagtaaa gaattggtca aaaccaatca aaactccccc aagtccaatc attacaggta
      241 aaactactag agccaggaga ctttttcgat aaagagacaa aaagtccttt ttcacaatcc
      301 tattgacaaa gacatagaaa cttggcagtg tcactagagc tactagctga accaaatgaa
      361 agagattctt gaccactgcg aaatggtgca gaccagctgc tgacgaacga aaatcaggca
      421 tctgtaagac ctgactaaaa ggattggtca gataattcat caagatatga aaattgtatt
      481 gaatggtttc tggttttaga tagactcgat tcgttaagtt tagccactga atctccatag
      541 gatagaaaat ccaagccaga taaatggtca gaaggatgga gagggagagg agaaagagca
      601 tagagcccca aaagatcaat ttagttttca tcaaaatccc actccgcaag gctagaaacc
      661 acatgtgtcg gtgcgattgg caggccagct acttcttctg ccttagtaaa acctgtcgtc
      721 accaagagcg ttggaatgcc attgtcaatc ccagcccgaa tatcagtcaa ataattgtcc
      781 ccaaccatga ttaactcttc acgttccaaa cctaagtgct caaccgcctt gtccataatg
      841 atggcatttg gttttccgat ataaaccggc ttcactcgtg tcgctacttc aagcagcgta
      901 atcagtgagc cagcacctgg caaaagaccg cgttccgtcg ggatgttgag gtcaggattg
      961 gttccgataa aatgggcacc cttttgaata gcaagagttg ctgtggcaaa tttttcatag
     1021 tcgacttgcc aatccagacc aactaccacg taggcaggtt tttccttgtc ttccacataa
     1081 ccagccgcct tgatggcttc cttgagtcct gcttctccga cgacatagac ggtcttttca
     1141 agccccaaat cattcatata gtcgatggtt gccaaagtcg ctgtgtagac agtcgatagg
     1201 ggcgtatcga tattaaaatt ctgagccaac atctccttaa cactctctgg agtgcgggtt
     1261 gtattgttgg ttacaaagag atagggaatg tcccgctttt gcaattcatg aacaaaagtc
     1321 tctccagcag ggattcggtc tttcccctta taaatggttc cgtctaaatc aattaaatag
     1381 cctttatatt tcatctattt ctccctaagc cttttttatt tcttgccaag taatgattgc
     1441 ttgggcattg ataaccccat cacttgtaat ttcatgcttg ctttccagtc cagtccgttc
     1501 aacagccgat gtaatcaccc cacctggtcg aacttccttg acatacttga ggttgatttt
     1561 cttgggaata tagtgggtca aaaaatccgc tcccatgacc tcaaaaatcc agtccaagta
     1621 tttactgtta ttgacatgac cattcatatc caagtcgtaa aaacgaacat ggtaatcctt
     1681 gctgatcggt tcttccaagg actcatactt cggtccacgg ataagttttt tatcaaaatc
     1741 agactggtaa ggagccacaa tctcaggttc aacaacatgg acttttcgac tgtcgcggtc
     1801 catgagaaca aaggtcgcca tcatgtggat gagctcctgc tccgcttcat tataaatagt
     1861 aaagcgacgg tagcaaaaaa gtcgattgta gctcaaggct tccgtttcga tggtaatttc
     1921 ttccgcaaaa cgaggcaaac gaaacacctc aatatcatat tctacgataa tccagaccag
     1981 attatattct tccaaaatgg ccttatcact aactcccagt tcaatcgact gcatccctga
     2041 aacttgcagt gacagcaaaa tcacatctgg aagtttgata tgaccgttca tatcagccat
     2101 atcaaaagga attttcattt tcatttgata agttaagccc atgatcctac tccaaaataa
     2161 atcgttctgc tacagtatct cccaaaaaga gacctctctt tgtcatgcga acgtggtcac
     2221 cctcaatctg catgaggcct tgttgaacca aatctctgac aatttctcca taaagtccag
     2281 caaaagactg tccaaatttt tcctcaaatc gcgccatgga aaccccggat ttcttgcgga
     2341 gtcccaagaa catttcttct tccatttgct ccttttgact caggtgatct tctgtaatac
     2401 aagcattgcc ttcctcaacc gcactgagat aatgacgaat gggaccatga tttttatagc
     2461 gtactccatt gacataacca gatgcccctg caccaatacc atagtattca gcattgtccc
     2521 agtacatgag attatgacga ctttcaaagc cgggtttgga gaaattagaa atctcataat
     2581 gctcaaaacc cgctcgctcc agctctgcaa tgatgtactc aaacatctcc gcttctagtt
     2641 cctccttagg cagaggcaat ttcccacgtc gcatccggtt cataaagacc gtatggtttt
     2701 ctaaaatcaa actatacaaa ctcatgtggg gaatatccaa tccaatggct ttagccacat
     2761 tttcctttac ttgctccatg gtctgaccag gcagagcata aatcaaatca atggagatgt
     2821 tgtcaaaacc agccagtttc aggcgatcga tattttcata aatatccttc tccaaatgac
     2881 tgcgcccaat ctttttcaac atcttatcat caaaggtctg gacacctagc gaaacacgat
     2941 tgacagccga atttttcaaa acagctatct tatccgcatc caaatcgcct ggattggctt
     3001 caatggtcaa ctcttccaag acagacaaat ccaagttttt agtcaagcca ttcagtaaca
     3061 cctccagttg cggagccgac agggctgtcg gtgttccacc accgatataa agggttgaca
     3121 acttttcaat atcataagaa cgaaactctt ccagcagatg ctctaaatag ctgtcgactg
     3181 gctgattttt gatgaagacc tttgaaaaat cacaataata acaaatctgg gtacaaaatg
     3241 ggatgtgcac ataggctgac gttggttttt tctgcatagt aattattata ccacaaagac
     3301 tagattccag ataaaaatca ccatccccag atacatagtc cgtccggaga tggtgatggt
     3361 ttattcttct gttatatcaa tcacaatctc ttctgagtca tcaagagctt cggctttttc
     3421 ttgccattgt tccttgagat tatttaattg attttttgat gcttctgtcg cttgaaaagc
     3481 ataggattta gcttgagcaa gtatactgtc cacagtgatt tcacctgact caacctgttc
     3541 ttttgttttc agaacaaaat ctgtagcctg ctccttaact tctgtcagtt tttcacagac
     3601 ttgctccttg gcatactccg gatcttctct caaatcatct agaaaatctt gagcctgact
     3661 gcaaacttgt ttgcccttat cacttgttaa aaacaaggca agagctgcac ctgaaacggt
     3721 tcctaaaagg attgaggata atttacccat aaggattctc cttttttatt ttttgaaaaa
     3781 tttacttgca agacgaagag ctgacagact tgcaccagtc ttgagtgttt ttgaaccagc
     3841 tgatgaagct ttcttgctca agacacgcgc atggtcattg aggtctgaaa cagatagaga
     3901 taaatctgca acagcactga agagtggatc aatcgtagcc accttgacat tgatatcatc
     3961 tgccaagaca ttgaccttag ccaacaactc attggtgtga tgcaaggtca catccacatc
     4021 tgaagtcaag gttttaatcg tcttttctgt ttcatcgatg acacgaccaa gcttttgtac
     4081 agtaatgatc agatagacca aaaagacaat cacagctagg gcaacaagaa tatatgcaac
     4141 ttctaacatt tagttttcct cctctgtaat atagtaaggg gccttctttc gattttgata
     4201 aataacgatc attataccga gaccgataaa gacaactgac agccattggg acactcgaag
     4261 gccgaagaac atgagactat ctgttcgcat accttcgatg accatacgac cgaaaccata
     4321 ccaaatcaag taaaaggccg tgatatgacc tcgtctgaga ctcttccatt tccgtctaaa
     4381 aatcagaatc aaggcaaagc caagcagatt ccatagagac tcataaagga aagtcggttg
     4441 atggtagctc ccctcaatat acatctggtc acggataaag ccaggtagat aatccagatt
     4501 atccactgtt gcaccataag cttcttggtt aaagaaatta ccccaacgcc ccaaactttg
     4561 agcaatcata acgctaggcg ccgcaatatc tagaaaatcc caagtattga tgagtttacg
     4621 gtcagcaaag atatagagca caagagcccc agttatcaaa ccaccgtaaa tggccaaacc
     4681 accattccaa atggcaaaaa tctctcctaa attctgacta tagtaatcaa atcggaaaat
     4741 aacatagtag agacgagctc ctaaaatagc caagggaaag gctactaaga taaaatctaa
     4801 aatatcgtct ggtatgatct tctttctagg tgcttctttc atggtcaaat aaaccgcaag
     4861 aatcaagcct gtcacaatac ataaggcata ccaacgaatg gctaggggtc ctagttgaat
     4921 agcaattgga tcaagcattt tgcacctcat ttcgagctat gagacttgtc agtcgttcgt
     4981 cgaacaaacg ggtagcatca aagcccattt ccttggcacg ataattcatg gcagctgcct
     5041 caatcacaac agagatatta cgacctgttt taactggaat acgaatacga ggaatggcta
     5101 cgccagaaac ttcaagttcc tctgcattgt ttccaagacg atcaaaggtc ttatgcgtat
     5161 cgtaattttc caaatagaca gcaagctgaa cctgtgaaga atccttgaca gcactcgcac
     5221 cgtagagact cataacatcg ataataccaa ccccacgaat ttcaatcaag tgtttcaaaa
     5281 tttcagctgg ttcaccccag agagtaatct catccttggc aaagatatcg acacggtcat
     5341 cggctaccaa acggtgacca cgtttgacaa gctcaagacc tgtctcgctc ttaccaattc
     5401 cactatctcc ctgaatcaag acgcccatcc cataaatatc catcaagaca ccgtgcacac
     5461 tggtacgttc tgccaaacga gaatccagat agctagataa ctctccagat aaacgactgg
     5521 tagctgcacg gctggttaaa atagcaatct tacattctct agcagccttt aacatctcct
     5581 ctggaaccac caaaccacgg gcaacaatga ctgctggtgt ctcaggtaga aacatttttt
     5641 tcaaaacttc ataacggctg ttggaaggca tgctgatcag ataagaccac tccttcatcc
     5701 ccaaaagttg aatccgctct ggtgtatagt agtcaaaata gcctgtcatt tcaagaccag
     5761 gtcgcgtaat atccgctgta ttgatttcct tttcaagcaa ttctggttca ccatagacaa
     5821 tatctagtct aagcttttca atcacttctt ttactaaaac cgacatcatt tcctccttca
     5881 aatgaattct tctctctatt atatcaaatt gtaggaggaa gtttcagatt tttgcaggct
     5941 ttggagtatt tatttaaaag aaaaaggact agatttctct agcctttcat ttcaattaga
     6001 attttttacg tttacgagct acttctgatt tacgtttacg ttttacagaa ggtttttcat
     6061 agaattcacg tttgcgtgtt tcttgaagag taccagcttt agtaaccgca cgtttgaaac
     6121 ggcgaagtgc gtcgtcaaga gattcattct tacgtactac tgttttagac atttttttca
     6181 ctcccttcaa gttcaatatc tatttcattc tacacgtaaa aggaataatt gtcaagcaaa
     6241 aatgcggttt aattttgatt ttttcttcca tttatacaag agttgaaagc gttcaataga
     6301 acattgcttt tttatttttc gagtaagcta agcgcttcag catctgcgat gatggttaca
     6361 tcagggtgat tttggaggct acttgcaggt aggttctcag tcactgggcc agatactgtt
     6421 ccggcaatgg cttctgcttt cgactcacca taagcaaaaa gaataataga cttggcatcc
     6481 aaaatgtttt taatccccat tgaaatagct tgggttggga cgtcttcaat cttggcaaag
     6541 aagcgagcat tggcttcgat agtagactgg tcaagttcta ctagatgcgt ttgactgtca
     6601 aatggagtac caggctcatt aaagccgata tgcccattgc gaccgattcc caagatttgg
     6661 aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt caacttcagc ttcagcattg
     6721 tccttaacac cacgaggcaa gaaactttct ttaaatggtt tttggttgaa caagttttct
     6781 tgcatgaagt aacgataaga ctgtggattg tccccatcaa ggcccacata ctcatcaagg
     6841 ttgacactgg ttagatttga aaaatcaagg tcactctcaa caatttcctt gtaaaattca
     6901 agtgggctac ttcctgtcgc aagtcctaga gtttgagctc cattagccaa tttttccttc
     6961 aaaatctcaa aagcaacttt tccaccttga acttggtttt caactttaat aactttcatc
     7021 ttttcatcct ccatttctgt ctatatttat tatatggtat agaccaattt ttgtcaagtg
     7081 aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat aatggatagg
     7141 attagaactt agaaagaatg aacaaatata tgaatacagc tgattttgat ttccacttgc
     7201 cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa ctcctcatcg
     7261 tcaaccgtga gacaggagaa atgcaagata aacatttcca ctctattatt gatatgctgg
     7321 aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc ctctatggtc
     7381 aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact agtggagacg
     7441 agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt atcagctttg
     7501 gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggagga cgcattgtcc
     7561 gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa atgcctctgc
     7621 caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc tacgccaagg
     7681 aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa ctgctggcag
     7741 aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga ctcggaacct
     7801 ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag ttctatcaac
     7861 tttctgagga agctgctgcc acccttcgct ctgtcaaaaa aaatggtggt cgtgtcatcg
     7921 ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt gatgggcaaa
     7981 tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag tggaaggtcg
     8041 tggatgcctt ctcaaccaac ttccacctgc caaaatcaac tctggtcatg ttggtttctg
     8101 cctttgcagg ccgtgaatta gtcttagatg cctaccacca ttccatccaa gaacactacc
     8161 gcttcttcag ttttggtgac gccatgttta tttattgaga aagaatttct ctaaatcttc
     8221 taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga aactctagct
     8281 agctgtagaa gaggcctagt acattgaaat taaaatgctt ccccctagct tcgaaaatat
     8341 tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc agcttcctat
     8401 actttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca tatttgttac
     8461 tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca ctactttgac
     8521 cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct cttcaaaccg
     8581 cgtcaacgtc accttggatt atatatgtga ctgactttgt cagttctatc tacaacctca
     8641 aagcagtact ttgagcaacc tgcgactagt tttctagttt gctctttgat tttcattgag
     8701 tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca gaagactagg cttgttcact
     8761 tttttatagt cgctataaga tgaccttatc tatagctttt tatatataat tatatattca
     8821 gacatactat tatcaatttt gtcgcaggga ggaatctgtt aacgcaccca ttcaccatta
     8881 tcattgactc tatagccatc tatacttgta ttgaccgcta actcacccga tgtatttaca
     8941 taataccatt taccaccaac ttggaaccat tgattgactt tcatagaacc gttgctgttg
     9001 aggtagtacc atgaaccatt aacttgtacc caacctgttg ccatggaacc atcagtatta
     9061 taaaaatacc acataccatt ttcttgtttc cagcctgttg ttggagcaac tgctttagct
     9121 ggttctactg ctacatctgt tccttggtta gatgtaacag atacaggata cgaaggaata
     9181 gatgattgct caggaacaac aactttttca ggttctctcg tccctctcct tatacgtctt
     9241 tttaccatct ctttagtaat ttgacgagaa gtagtttctt caattgttcc atcacgttca
     9301 tctacagcat agattgtagt aagagtaatt ttaccaattt ctcctacttc ttgaccttta
     9361 tcaagagttg ggtcatcgag atattctgtt tcgattggaa tttcttggac aataacttgg
     9421 ggcttggttc cttttttaac aactcttgtt tgagagtctt ctttttgact taaagtactc
     9481 tcagttactt gtccactctt tccatctaca tcataagtta tcgttgtaac tgttttccca
     9541 ttctttccta gagtaatctc ttgctcctgt cctgcagaaa ggtcattgtc tgcttcatat
     9601 ttagtagcac atggaacaag aacttcttca accttgcttt tagctggaac tttgataact
     9661 gtatccgtgg cttcttttct atcaacagta accttttcgg taacatgacc agtctctgga
     9721 ttaacatcgt aggtccttgt cgtggttaca tagccatcct ctccgtcaat tgtaacagga
     9781 ttttcactac ggtattttgt ttcatctttt tcaaaacgaa ttcgcgtact tgaaattttc
     9841 ttggttacta ccttaggttt agtcgctact tttacaataa tatccccatt gtcagcgtca
     9901 tcatactcta ttccctcttc tttatctcta gtatcatctc tgacatattg aatcccatca
     9961 gcagcatgag caaaacttgt attcagattc ctcctaaaaa taaagttagc ccgattaccg
    10021 cagaaccaaa aatctttccg agtttacgta ttgcatagcg cttattagta ttagattttg
    10081 ccattacatc ctacttctag tatagcatct tttctatcaa acgttaaaca atatacttta
    10141 tatataaaat agacttagaa tgatatattt actattgaac taacacttta actatatcgt
    10201 aatcaatctc atatataaag gattgcagac atctgatcta aatacatgct aatatattta
    10261 gatacaaaca ttccaacttg ataattttca ctcatctttc atcattcctt acacaacgat
    10321 gcagt
//
LOCUS       DAVMAF010000067        10234 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMAF010000067 DAVMAF010000000
VERSION     DAVMAF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.068"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            1..475
                     /locus_tag="VYO97_001666"
                     /pseudo
     CDS             1..475
                     /locus_tag="VYO97_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            375..821
                     /locus_tag="VYO97_001667"
     CDS             375..821
                     /locus_tag="VYO97_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1754431.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(959..2263)
                     /gene="eno"
                     /locus_tag="VYO97_001668"
     CDS             complement(959..2263)
                     /gene="eno"
                     /locus_tag="VYO97_001668"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1754432.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2427..2873
                     /locus_tag="VYO97_001669"
     CDS             2427..2873
                     /locus_tag="VYO97_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1754433.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2870..3673)
                     /locus_tag="VYO97_001670"
     CDS             complement(2870..3673)
                     /locus_tag="VYO97_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1754434.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3682..4011)
                     /locus_tag="VYO97_001671"
     CDS             complement(3682..4011)
                     /locus_tag="VYO97_001671"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1754435.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3956..4327)
                     /locus_tag="VYO97_001672"
     CDS             complement(3956..4327)
                     /locus_tag="VYO97_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1754436.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4386..5819)
                     /gene="glgA"
                     /locus_tag="VYO97_001673"
     CDS             complement(4386..5819)
                     /gene="glgA"
                     /locus_tag="VYO97_001673"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1754437.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5816..6955)
                     /gene="glgD"
                     /locus_tag="VYO97_001674"
     CDS             complement(5816..6955)
                     /gene="glgD"
                     /locus_tag="VYO97_001674"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1754438.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6945..8087)
                     /locus_tag="VYO97_001675"
     CDS             complement(6945..8087)
                     /locus_tag="VYO97_001675"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1754439.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8077..10005)
                     /gene="glgB"
                     /locus_tag="VYO97_001676"
     CDS             complement(8077..10005)
                     /gene="glgB"
                     /locus_tag="VYO97_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1754440.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3117 a         2257 c         1800 g         3060 t
ORIGIN      
        1 gctgtcatcc aactaccatc cactatgcgc tcaaagctat gggctacact cgaaaaaaaa
       61 gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa
      121 tagtttaaag cacctagcac aggtctaaca aatgaatggt gagttaatcg ctccaatgac
      181 ttacgaagag acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc
      241 aacattaaac acaccatcgg ttattattat ggataatgca agattccata gaatggggaa
      301 gctagaactc ttgtgtgaag agtttgggta taaactttta cctcttcctc cctactcgcc
      361 tgagtacaat cttattgaga aaacatgggc tcatatcaaa aagcacctca aaagggtatt
      421 accaagttgc aatacctttt atgaggctct tttgtcttgt tcttgtttca attgactata
      481 taaattgtct aagcgaaaca accgataaga attggcacaa aagcgaccgt atttttgtta
      541 ccaatacagg aaaaccagtt catagttcta tcttgagcaa gtctctccag cgagcaaacg
      601 aacgccttaa aaaaccaatt cccaaacatc tgtcccctca catcttcaga cacaccacta
      661 ttagcatctt atcagaaaat aaaattcctt taaaaacaat cacggacagg gttggtcatt
      721 ccgactctga agtcactact tccatctaca cccacgtcac aaagaacatg aaagatgaag
      781 caatcaatgt actggataaa gttatgaaaa agatttttta aaaagttttg tccctttttt
      841 gtcctctaaa tacaaaaata gcccttcgga taaaatccga ggggctagaa acgttgttaa
      901 atcaacggcc gaacttttga atttcatggt tcgggataaa atagttcact gaactatttt
      961 attttttaag gttgtagaat gatttcaatc cacgatattc agctacttca ccaagttggt
     1021 cttcgatacg aagcaattgg ttgtatttag cgatgcggtc tgtacgtgaa agtgaaccag
     1081 tcttgatttg tcctgcgtta gttgcaactg caatatcagc gattgttgaa tcttcagttt
     1141 cacctgaacg gtgtgataca acagcagtgt aaccagcttc tttagccatt tcgatagctt
     1201 caaaagtttc agtaagagta ccgatttggt taactttgat aaggattgag ttagcagcac
     1261 cttcttggat accacgtgca aggtagtcag tgtttgttac gaagaagtcg tcaccaacaa
     1321 gttgtacttt cttaccaaga cgttcagtaa gagctttcca accatcccag tcgttttcat
     1381 ccataccatc ttcaatagtg atgattgggt atttgttaac caattcttca aggtagtcga
     1441 tttgttctgc agatgtacga acagcagcac cttcaccttc aaatttagtg tagtcgtaaa
     1501 ctttacgttc tttatcgtag aattctgatg aagcacagtc aaatccgata aatacgtctt
     1561 tacctggtac atatccagca gcttcaatcg cagcaaggat agtttcaaca ccatcttcag
     1621 ttccttcgaa acgaggagcg aatccacctt cgtcacctac ggcagtttcc aaaccacgtg
     1681 atttaaggat tttcttaaga gcgtggaaga tttcagcacc gtaacgaagg gcttctttaa
     1741 atgttggcgc accaactggc aagatcatga actcttggaa agcgattgga gcgtcagagt
     1801 gagaaccacc gttgatgatg ttcatcattg gagttggaag aactttagtg ttgaatccac
     1861 caaggtagct gtaaagtggg atttcaaggt agtcagcagc agcacgagct acagcgatag
     1921 acacaccgag gattgcattc gcacccaatt tacctttgtt aggagtaccg tcaagtgcaa
     1981 tcatagcacg gtcaatagct tgttgatcac gtacatcgta gccaatgata gcttcagcaa
     2041 tgatgttgtt tacgttgtca acagcttttt gtgtaccaag accaccgtaa cgagatttgt
     2101 caccgtcgcg aagttcaact gcttcgtgtt caccagtaga agctcctgat ggaaccatac
     2161 cacgtccgaa agcacctgat tcagtgtaaa cttctacttc aagtgttggg ttaccgcgtg
     2221 agtctaggac ttcgcgagcg taaacatcag taataattga cattttttac tctccttatg
     2281 agttaaattt tttacacctc tataatacct taaaacccct cctttttcaa gaaaaaacgt
     2341 tatctttgtg caacttttcc ttaactttat aaagtaatcg ctttcttttg tctgttttat
     2401 tctaactttt atgatatact gttttcatga cagatttatc aaaacaatta cttgaaaaag
     2461 ctcatggtgg gttaaaaata aatccggatg agcaaagacg ctatcttggt acttttgagg
     2521 aaagagttct tggatatgta gatattgaca cagcaaatag ccctcagtta gaaaaaggct
     2581 ttttatttat tttagaaaac cttcaggaaa aagcaaagcc actatttgtg aagatttcac
     2641 caactatcga atttgataag caagttttct acttaaaaga agcaaaagaa actgatagtc
     2701 aagccaccat agtatctgaa gagcatatta cttctccttt tggcctggtt attcatagca
     2761 atgcaccagt tcaagtagaa gaaaaagacc ttcgacttgc ttttccaaaa ctttgggaag
     2821 ttaaaaagga agaaccagcc aaaacatcct tatggaagaa atggtttagc taaatcttgc
     2881 acatatttaa taagtgccca atattggcag ccgtgcgctc cagatagaga ctggcatttt
     2941 tcaaactatc ttctaaaggt tcacttttct ccaaaataga aaagacagct tggatatttt
     3001 caaatggtag gggaggtaaa tcttcagcaa gactaccgca aatagcaata acaggaactc
     3061 caacaggggt tctttttgca acacctatag gcgctttccc agcaaagctt tgactatcaa
     3121 gtcttccttc tccaacaaca accaagtcag catctgaaac tttcttatca aagttgatta
     3181 agtccaagca ggtatcaatt ccagacacga tacttgcctg agcaaaggca cacaaaccag
     3241 cagcaatccc cagtcctcca tcattactgg ccgtgccacc aacaccgata taaatatctt
     3301 taatcccttt agaaatgaga tggagaatca actctccaat accacaagtt tggatttgaa
     3361 gtggatttcg tttctctagc ggaatttttc caagaccaac caagtcagct acttcaaata
     3421 gtgccagttc ccctttttga aaatagcgca tggcttcttt ttgtccaaaa gggtctgtca
     3481 cttggatcca tttttctttt aggtcaagag aatgtcggat agcatctaca gtaccttctc
     3541 ccccatcacc aacagggcag aggagacatt ctacatctgc tatcgattgt tggaagcctc
     3601 tttttattgc ttcagctacc tgttgagctg tcaagctttc cttaaacgaa tccggtgcaa
     3661 ttacaatctt catattttcc ctcattctaa acagtcaatc aaaggaagaa cttctaaaaa
     3721 atccctcttg tcaacatgat gtggtatttc ttttttgagc atttctttgg aacaaaaggc
     3781 gattcctaac tccgccgact tcaacattaa tagattatta accccatcac cgattgccac
     3841 cgttctttct ttagaaagtt ttagtttctt tctccatttt tccagagttt cttttttgac
     3901 ctggggactt ataatttgtc caactaattt tcctgttaaa agaccttctt tgacttcaag
     3961 ctggttggca gtgaaatagg caataccaag ggattttgct aatctctcca actattggtg
     4021 taaatccacc agacaccaga ccaactagga tgccattctt ttggagaata gagatgaact
     4081 ctgggacatt tagcgataga tgaattgagt tgaagacgtt atcaaagacc aaaataggaa
     4141 gaccttccaa caaggacact ctttttctta aactgctttc aaagaccaac tctcctcgca
     4201 ttgctcgact tgtaatctgc gaaatttccg cctcatgacc tgcctctctc cctaaaagat
     4261 caatcacttc ttctaggatt aaggttccat ctacatccaa aacacacaag ccttttactt
     4321 gagacatcag ttctcctctc taaacagcct aaaaatcgta tgaagtcatc atacgatttt
     4381 atctattaat taactaaact atggtacaag tcaaggtatg acttgcaggc tgtatcccat
     4441 gagaagtcac tctccatagc ttgtttttgt aggtttctcc aaatgtctgg atggtttcta
     4501 tacaagtcca atgctgtttg gaaagtccaa tttaaccaat aaggagatag attgtcaaag
     4561 ctaaagccag taccgcttcc ttcgattgga ttgaaagcgc gaactgtatc tcgcaagcct
     4621 ccaacttcat ggaccaatgg caaggttcca taacgcatag ccatcatttg agacaagcca
     4681 cacggttcaa aacgacttgg catgaggaag aggtcacaag cagcgtagat ttcctgagca
     4741 agtttgacat caaaagtgat atttgctgat agcttgtctg ggtaaatctg agcaaaccat
     4801 gagaaagctc cttcaaaggc tggatcgcca gttcccaaaa gaacaatctg aacatcttct
     4861 tgcaagatat ggtgaagact ttcgaccacc acatcaaaac ctttttgacg tgtcaaacga
     4921 gaaacaattc ccaccagtgg aacgtctgct ctaacaggca agccaactct ttcttgcaat
     4981 tttgccttat ttttggcttt cccagacaaa tcttcctgat tgaaatgata gtctaaaaga
     5041 gcatccgtct gaggattata aagatcagca tcaatcccat tcacgatacc agatacttta
     5101 ccagactcca ttttaagaat ctgatccaaa ttacatccaa actgactagt cataatttca
     5161 tgagcatagc taggtgaaac ggttgaaaca cggttcgcat agagaatacc tgccttcatc
     5221 cagttcagac agttgttcca tcgaagggtg ccatcagcgt aacgttcaag gccaactcca
     5281 aacaaatcac ccaacattcc ttctgaaaat tgtccttgga attctaaatt atgaatggtt
     5341 aaaactgttt caatgtcctc ataggcttga atccaacagt atttttcctt caacaagaaa
     5401 ggaatcatag ctgtatggta gtcatgaaca tggagaagat caggaataaa gtcaatcctt
     5461 tccatagcct caatggcagc cagttggaaa aaggcaaagc gttctccgtc atcaaaatca
     5521 ccgtaaacat gaccacggaa gaaataatat tgattgtcaa taaagtagaa ggttacacca
     5581 tttaatactg ttttcttaat tccacaatac tgtctacgcc aaccaacgct cacctcaaaa
     5641 tgaagcacat cttcaatctg atttccaaat ttagcctcta ccatatcata gtagggtaaa
     5701 atcactgcaa cttcgtgccc agcttttacc agtgattttg gaagagcgcc aatgacgtct
     5761 cccaaaccac ctgtttttga aaagggtgca ccctctgctg ctacaaataa aattttcatg
     5821 aatgaatatc ctctgttact ttagcacctt tcttaaccac aactggatgt tctgcagttc
     5881 ctcgaatcac aacaccaagc tcaacttcaa cccctttgtc caagatagca tattcgacct
     5941 gagccccttc tccaataaca acacgaggga agagcaggct atctttaacc aagctatcct
     6001 tatggacatg aatattacgt gatagaacag aattagctac ttgaccttca ataatactac
     6061 cagaggcaaa ctgagaagtg cttaccttag atgtattagc ataataagtt ggctcttcgt
     6121 ttttgacctt tgtataaatc ttttggtttg gtgagaaaag agaatagaat ttttgtgatt
     6181 caagcatatc gatatttgct tgataataag atttaacaga gtgaatattg gctagatagc
     6241 ccgtgtactc gtaggcaaaa gctccctctt ttacagccaa atcccgtaaa acatagcgca
     6301 atttctctgg atgttctttt ttagcttctt cttccaagtg ttcaatcaac caaggtgtat
     6361 caacgacaaa gatatctgta gacatattga acgtttcagc tgttgacttg ctatcaaaga
     6421 gtttatgaga aagaacatgg tctgtttcat ctacatccaa gattgcattt acttctgaaa
     6481 tatctttctt agctagtttt ttataaacta cagtgatagg ctcttttgtt gtactatgta
     6541 ggtggaaaac ttggttcaaa tcaatgttaa taagaacatc gcagttgagg gcaaccgttt
     6601 ggtttgagcc agaacgtttc aaataagtaa gaagctgttg gtagtattct tttccaactg
     6661 tactactttc tacacgggta ttgtaaattc ctagatagta atggctaaga agggttgata
     6721 agccccactc gcgccctgaa cgaatatggt caaagactga gctgatatta tcctgctgga
     6781 aaataccaaa gacactacga acacctgcat tagcaaggct tgaaagtggg aagtcaatca
     6841 aacgatattt cccaccaaat ggcaaacttg ctactggacg gtggtccgtc aatgtcgaca
     6901 tatcgtgaaa accaactgta tttcctaaaa tggcagaata tttatcaatc ttcatctgtt
     6961 gctaccccca ctacttcatt atatcctaca acttgtactt catctgttcc atcaatttcg
     7021 acaccgtcag aaataatcgc accttcacca ataatggcac gtttaatctt agctccttga
     7081 ccaataatag ctccactcat gataactgaa tcaaggactt ccgctccttc gcgaacttgc
     7141 gcgcctgttg aaaggataga atgtttaata gttccatcaa cgaaacatcc gtctacaact
     7201 aatgagtctt ccacatgagc atttgccccg aggaagtttg gtggtgaaat caagtttctt
     7261 gagtaaatct tccattgacg gttacgacta tccaaggcat tttctggaga aatatactcc
     7321 atgttcgctt cccaaagtga ctcaatagta ccaacatctt tccaataacc actaaattcg
     7381 taagcataaa cactttcacc tgactcaagg taatttggaa tgacattttt accaaagtct
     7441 gacatgccaa ccttgctctt ttcagcagcg actaacatat tacgaaggcg ttgccaatca
     7501 aaaatgtaga ttcccataga agcttttgta gatttaggtt gagctggttt ttcttcaaat
     7561 tcaacaatac gattgttagc atctgtgttc atgataccaa aacggctcgc ttctttaaga
     7621 gggacgtcta aaactgctac tgtcaagctg gcattattat ccttatgaga ctggagcata
     7681 tcatcatagt ccattttgta gatgtggtcc ccagacaaaa tcaagacata ctcaggattg
     7741 acactgtcga tatagtcgat attttggtaa atagcgtgac tagttccctc aaaccaacga
     7801 tttccttcac ttgcagaata aggttgaaga atagagacac ctgaattaat accgtctagt
     7861 ccccagcttg aaccattccc aatatggttg ttgagagcaa gtggttgata ctgtgtaacc
     7921 accccaacat tatgaatccc tgagttggca cagtttgata gggcaaagtc aatgatacgg
     7981 tagcgcccac caaattgcac agctggtttt gcgatgcttt gagtgagttt accgagacga
     8041 gttccttgcc caccagcaag aatcaaagct aacatttcat ttttcatttt ctactccttt
     8101 ttggttttta tttgtgacgg ttttagtaga tttcaagcga cgtttgattt tccatacact
     8161 tgctcccata gccggtaggg taaaggttaa ggtctgctca taatctttcc ataggccttc
     8221 ttgcgtttga acagtttgat tatgttcttt ccaaacgcct ccccactctt ccaactcagt
     8281 attccatact tcttcgtaaa ttcctgcaac gggtagtccg attgtaaaat ctttccgctc
     8341 aacaggtacc atgttaaaga tacagactaa catttctccc tttttaccct tacgaataaa
     8401 ggaaagaaca ctctggtctc gattatccgc atcaatgatt tcaataccat catagctggt
     8461 atcgatttcc cacagacagc gatgatcttt gtaaaactgg tttagctgag aagtgaaata
     8521 cttcatctta gcattcattg ggtcttctag gttagaccat tccaactgtt cttcagattt
     8581 ccattctagg aattgaccgt attcgctacc catgaagagc aatttcttac cagggtgaca
     8641 aatttggtac gtatagagat tgcgcaagcc tgcgaattga ttgtaacgat ctccccacat
     8701 cttatgcatc atactcttct tgccatgaac cacttcatcg tgcgagaatg gcaagagata
     8761 attctccttg aaaacataca taaagctgaa agtcaccagg ttaaagtcat atttacgata
     8821 aatcggatct tcttcgtaga aacggaggat atcattcatc cagcccatgt tccatttgta
     8881 gtcaaatcct agaccaccaa tctctttcat tcccgtaatc ttggtcgcag acgaactttc
     8941 ttctgcaatc atcatcacat ctggatattc taacttaata acttcattca agcgttgaag
     9001 aaaataataa ccttcatagt tgagatttcc gccatcttta ttaggtgtcc atggagcatt
     9061 atcatagtcc aaatagagca tgttgctaac agcatccaca cgaataccat ccaaatgata
     9121 gacatcaatc caatgcttaa tgcaagaaat taagaaggac tggacttcat tttttccaag
     9181 gtcaaaatta agggcacccc aaccatggtt atgagcctta ttatggtctt ggtattcaaa
     9241 agtcggtgtc ccatcataat aggctaaggc atcatcgttg atggtaaagt gacctggtac
     9301 ccagtccaca ataaccccaa tattatgggt atgacactcc tcgacaaaat cttgaaactc
     9361 ctctggtctg ccataagcat gctctaaagc gaagtaaccc ataagctgat acccccaact
     9421 caagcccaaa ggatgggaca tcaagggcat aaactcaata tgagtatagt tcatttcaac
     9481 gagataagga atgagttcat ccttgagctg ggcaaaacta taaggactgc catcagaatt
     9541 tcttttccat gatccagcgt gaacttcata aatattgaca ggacgctctt caaagcccca
     9601 acgttttctt cgtgccagcc aaagtccatc cttccatttc ttctcaggaa gctctgttaa
     9661 gattgcccct gttcctggac gagcctcata cctgacagca aaagggtcaa tcttcatcag
     9721 ttgatgacca ttttgacgtg tgacatgata tttgtaaata tgcccttctt gagccatatt
     9781 ggtaaagact tcccagaccc caaaatcatt tcttaccatt ggaatctgat tttcaatcca
     9841 gttggtaaaa tcaccaacca agtgaacagc ctgagcatta ggtgcccaaa cacggaaggt
     9901 atagccatgc tctccattta gttcttccct atgtgctcct agataatgtt ggagataaaa
     9961 attttcaccc gtcataaagg tttttaatgc ttctctatta tccatatact ccccttctct
    10021 tgtaagcgtt ttctatgttt ttattatact acctttttag agaagattca agtaaattac
    10081 tatacttctt taattatttt gaaaatctac aacaagttca cttactcgtt caattgtaaa
    10141 tcaatatttt ttcaaaaaat tgcgaaaacg cctttctttt tctactatag tgaaatgaaa
    10201 taaaacatgc gcaagtcgat taaggaattt aatc
//
LOCUS       DAVMAF010000068         9885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMAF010000068 DAVMAF010000000
VERSION     DAVMAF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.069"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            147..5087
                     /locus_tag="VYO97_001677"
     CDS             147..5087
                     /locus_tag="VYO97_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1754441.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSRANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKRFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EFNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKAAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5177..6373
                     /locus_tag="VYO97_001678"
     CDS             5177..6373
                     /locus_tag="VYO97_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1754442.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6509..7036
                     /locus_tag="VYO97_001679"
     CDS             6509..7036
                     /locus_tag="VYO97_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1754443.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7113..7469
                     /gene="glnR"
                     /locus_tag="VYO97_001680"
     CDS             7113..7469
                     /gene="glnR"
                     /locus_tag="VYO97_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1754444.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7506..8852
                     /gene="glnA"
                     /locus_tag="VYO97_001681"
     CDS             7506..8852
                     /gene="glnA"
                     /locus_tag="VYO97_001681"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1754445.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8948..9067)
                     /locus_tag="VYO97_001682"
     CDS             complement(8948..9067)
                     /locus_tag="VYO97_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1754446.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9556..9885
                     /locus_tag="VYO97_001683"
                     /pseudo
     CDS             9556..9885
                     /locus_tag="VYO97_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3202 a         1790 c         2152 g         2741 t
ORIGIN      
        1 tagatttgtg aaaacagcta ttgttttttg tctaaaaatg ttttaggata aaaatgtgat
       61 tatcatggtt ctagagtttg ataaaaaact acagaatata aagcgctttc ttgaaaacaa
      121 caaaatcaat cttttaggag gagaaaatga agaatccatt ttttgaaaga cgttgtcgtt
      181 acagtattcg taagttatca gtaggagcct gctcgctgat gattggtgct gttttatttg
      241 ctggtccagc cttggctgaa gaaactgcag ttcctgaaaa tagcagagct aatacagagc
      301 ttgtttcagg agagagtgag cattcgacca atgaagctga taagcagaat gaaggggaac
      361 atgctagaga aaacaagcta gaaaaggcag aaggagtagc gacagcatct gaaactgcag
      421 aagcagctag cgcagctaaa ccagaggaaa aagcaagtga ggtggttgca gaaacaccat
      481 ctgcagaagc aaaacctaag tctgacaagg aaacagaagc aaagcccgaa gcaactaacc
      541 aaggggatga gtctaagcca gcagccgaag ctaataagac tgaaaaagaa gtccagccag
      601 atgtccctaa aaatacagaa aaaacattaa aaccaaagga aatcaaattt aattcttggg
      661 aagaattgtt aaaatgggaa ccaggtgctc gtgaagatga tgctattaac cgcggatctg
      721 ttgtcctcgc ttcacgtcgg acaggtcatt tagtcaatga aaaagctagc aaggaagcaa
      781 aagttcaagc cttatcaaac accaattcta aagcaaaaga ccatgcttct gttggtggag
      841 aagagttcaa ggcctatgct tttgactatt ggcaatatct agattcaatg gtcttctggg
      901 aaggtctcgt accaactcct gacgttattg atgcaggtca ccgtaacggg gttcctgtat
      961 acggtacact cttcttcaac tggtctaata gtattgcaga tcaagaaaga tttgctgaag
     1021 ctttgaagca agacgcagat ggtagcttcc caattgcccg taaattggta gacatggcca
     1081 agtattatgg ctatgatggc tatttcatca accaagaaac aactggagat ttggttaaac
     1141 ctcttggaga aaagatgcgc cagtttatgc tctatagcaa ggaatatgct gctaaggtaa
     1201 accatccaat caagtattct tggtacgatg ccatgaccta taactatgga cgttaccatc
     1261 aagatggttt gggagaatac aactaccaat tcatgcaacc agaaggagat aaggttccgg
     1321 cagataactt ctttgctaac tttaactggg ataaggctaa aaatgattac actattgcaa
     1381 ctgccaactg gattggtcgt aatccttatg atgtatttgc aggtttggaa ttgcaacagg
     1441 gtggttccta caagacaaag gttaagtgga atgacatttt agacgaaaat gggaaattgc
     1501 gcctttctct tggtttattt gccccagata ccattacaag tttaggaaaa actggtgaag
     1561 attatcataa aaatgaagat atcttcttta caggttatca aggagacccc actggccaaa
     1621 aaccaggtga caaagattgg tatggtattg ctaacctagt tgcggaccgt acgccagcgg
     1681 taggtaatac ttttactact tcttttaata caggtcatgg taaaaaaagg ttcgtagatg
     1741 gtaaggtttc taaggattct gagtggaatt atcgttcagt atcaggtgtt cttccaacat
     1801 ggcgctggtg gcagacttca acaggggaaa aacttcgtgc agaatatgat tttacagatg
     1861 cctataatgg cggaaattcc cttaaattct ctggtgatgt agccggtaag acagatcagg
     1921 atgtgagact ttattctact aagttagaag taactgagaa gaccaaactt cgtgttgccc
     1981 acaagggagg aaaaggttct aaagtttata tggcattctc tacaacttca gactacaaat
     2041 tcgatgatgc agatgcatgg aaagagctaa ccctttctga caactggaca aatgaagaat
     2101 ttgatctcag ctcactagca ggtaaaacca tctatgcagt caaactattt ttcgagcatg
     2161 aaggtgctgt aaaagattat cagttcaacc taggacaatt aactatctcg gacaatcacc
     2221 aagagccaca atcgccgaca agcttttctg tagtgaaaca atctcttaaa aatgcccaag
     2281 aagcggaagc agttgtgcaa tttaaaggca acaaggatgc agatttctat gaagtttatg
     2341 aaaaagatgg agacagctgg aaattactaa ctggctcatc ttctacaact atttatctac
     2401 caaaagttag ccgctcagca agtgctcagg gtacaactca agaactgaag gttgtagcag
     2461 tcggtaaaaa tggagttcgt tcagaagctg caaccacaac ctttgattgg ggtatgactg
     2521 taaaagatac cagcctacca aaaccactag ctgaaaatat cgttccaggt gcaacagtta
     2581 ttgatagtac tttccctaag actgaaggtg gagaaggtat tgaaggtatg ttgaacggta
     2641 ccattactag cttgtcagat aaatggtctt cagctcagtt gagtggtagt gtggatattc
     2701 gtttgaccaa gccacgtacc gttgttagat gggtcatgga tcatgcaggg gctggtggtg
     2761 agtctgttaa cgatggcttg atgaacacca aagactttga cctttattat aaagatgcag
     2821 atggtgagtg gaagctagct aaggaagtcc gtggcaacaa agcacacgtg acagatatca
     2881 ctcttgataa accaatcact gctcaagact ggcgcttgaa tgttgtcact tctgacaatg
     2941 gaactccatg gaaggctatt cgtatctata actggaaaat gtatgaaaag cttgatactg
     3001 agagtgtcaa tattccgatg gccaaggctg cagcccgttc tctaggcaat aacaaggtac
     3061 aagttggctt tgcagatgta caggctggag caactattac cgtttatgat aatccaaatt
     3121 ctcaaactcc gctcgcaacc ttgaagagcg aagttggagg agacctagca agtgcaccat
     3181 tggatttgac aaatcaatct ggtcttcttt attatcgtac ccagttgcca ggcaaggaaa
     3241 ttagtaatgt cctagcagtt tccgttccaa aagatgacag aagaatcaag tcagtcagcc
     3301 tagaaacagg acctaagaaa acaagctacg ccgaagggga ggatttggac cttagaggtg
     3361 gtgttcttcg agttcagtat gaaggaggaa ctgaggacga actcattcgc ctaactcacg
     3421 caggtgtatc agtatcaggt tttgatacgc atcataaggg agaacagaat cttactctcc
     3481 aatatttggg acaaccggta aatgctaatt tgtcagtgac tgtcactggc caagacgaag
     3541 caagtccgaa aactattttg ggaattgaag taagtcagga accgaaaaaa gattacctag
     3601 ttggtgatag cttagacttg tctgaaggac gctttgcagt ggcttatagc aatgacacca
     3661 tggaagaaca ttcctttact gatgagggag ttgaaatttc tggttacgat gctcaaaaga
     3721 ctggtcgtca aaccttgacg cttcgttacc aaggtcatga attcaatttt gatgttttgg
     3781 tatctccaaa agcagcattg aacgatgagt acctcaaaca aaaattagca gaagttgaag
     3841 ctgctaagaa caaggtggtc tataactttg cttcaccaga agtaaaagca gctttcttga
     3901 aagcaattga agcggccgaa caagtgttga aagaccatga aactagcacc caagatcaag
     3961 tcaatgaccg acttaataaa ttgacagaag ctcataaagc tctgaatggt caagagaaat
     4021 ttaaggaaga aaagacagaa cttgatcgct taacaggtga ggttcaagaa ctcttggctg
     4081 ccaaaccaaa ccatccttca ggttctgccc tagctccgct tcttgagaaa aacaaggcct
     4141 tggttgaaaa agtagatttg agtccagaag agcttgcaac agcgaaacag agtctaaaag
     4201 atctggttgc tttattgaaa gaagacaagc cagcagtctt ttctgatagt aaaacaggtg
     4261 ttgaagtaca cttctcaaat aaagagaaga ctgtcatcaa gggtttgaaa gtagagcgtg
     4321 ttcaagcaag tgctgaagag aagaaatact ttgctggaga agatgctcat gtctttgaaa
     4381 tagaaggttt ggatgaaaaa ggtcaagatg ttgatctctc ttacgcttct attgtgaaaa
     4441 tcccaattga aaaagataag aaagttaaga aagtattttt cttacctgaa ggcaaagagg
     4501 cagtagaatt ggcttttgaa caaacggata gtcatgttat ctttacagca ccacacttta
     4561 ctcattatgc ctttgtttat gaatctgctg aaaaaccaca acctgctaaa ccagcaccac
     4621 aaaacaaagt ccttccaaaa cctacttatc aaccggcttc tgatcaacaa aaggctccta
     4681 aattggaagt tcaagaggaa aaggttgcct ttcatcgtca agagcatgaa aatactgaga
     4741 tgctagttgg ggaacaacga gtcatcatac agggacgaga tggactgtta agacatgtct
     4801 ttgaagttga tgaaaacggt cagcgtcgtc ttcgttcaac agaagtcatc caagaagcga
     4861 ttccagaaat tgttgaaatt ggaacaaaag taaaaacagt accagcagta gtagctacac
     4921 aggaaaaacc agctcaaaat acagcagtta aatcagaaga agcaagcaaa caattgccaa
     4981 atacaggaac agctgatgct aatgaagccc taatagcagg cttagccagc cttggtcttg
     5041 ctagtttagc cttgaccttg agacggaaaa gagaagataa agattaaata tcgaaaaatc
     5101 ttgtgaaatc tttccttata tttccaaagt gtgatataat agtttcgaat aaaataaata
     5161 aaggagtttt tgtaacatgg caaaacttac tgttaaagac gttgacttga aaggtaaaaa
     5221 agtcctcgtt cgtgttgact tcaacgtacc attgaaagat ggcgtaatca ctaacgataa
     5281 ccgtatcaca gcagctcttc caactattaa gtacatcatc gaacaaggtg gacgtgcaat
     5341 tcttttctct caccttggac gtgtgaaaga agaagctgat aaagctggta aatcacttgc
     5401 tcctgtagca gcagacttgg cagcaaaact tggtcaagat gttgttttcc caggtgtcac
     5461 tcgtggtgct gaattggaag cggcaatcaa cgctcttgaa gatggacaag ttctcttggt
     5521 tgaaaacact cgttacgaag atgttgacgg caagaaagaa tctaaaaacg atcctgaact
     5581 tggtaaatac tgggcatcac ttggagatgg tatcttcgta aacgatgcat tcggtacagc
     5641 tcaccgtgca cacgcatcta acgttggtat ctcagcaaac gttgaaaaag cagttgctgg
     5701 tttccttctt gaaaacgaaa ttgcctacat ccaagaagca gttgaaactc cagaacgtcc
     5761 attcgtggct atccttggtg gttcaaaagt ttcagacaag atcggtgtta tcgaaaactt
     5821 gcttgaaaaa gctgataaag tccttatcgg tggtgggatg acttacacat tctacaaagc
     5881 acaaggtatc gaaatcggta actcacttgt agaagaagac aaattggatg ttgcgaaagc
     5941 tcttcttgaa aaagcaaatg gtaaattgat cttgccagtt gactcaaaag aagctaacgc
     6001 atttgctggt tacactgaag tgcgtgacac tgaaggtgaa gcagtttctg aaggcttcct
     6061 tggtcttgac atcggtccaa aatctatcgc caaatttgac gaagctttga ctggtgccaa
     6121 aacagttgta tggaacggac ctatgggtgt atttgaaaac ccagatttcc aagctggtac
     6181 aatcggtgtg atggacgcta tcgtgaaaca accaggagtt aaatcaatca tcggtggtgg
     6241 tgactcagct gccgcagcga ttaaccttgg ccgtgcagac aagttctcat ggattagtac
     6301 gggtggtgga gcatcaatgg aacttcttga aggtaaggtt cttccaggac ttgcagcctt
     6361 gacagaaaaa taagatttta taaacaaatc aaagaagaga gggatgaaag ttcctctttt
     6421 cttttgctta aaataaaaac gcttcctctc aactattact cataaaaatc accgatttat
     6481 gataaaatgg aaatagaaag ttgagattat gagttatttt aaaaaatata aattcgataa
     6541 atcccagttc aaacttggta tgcgaacctt taaaacaggt attgctgttt ttctagttct
     6601 cttgattttt ggcttttttg gctggaaagg tcttcaaatt ggtgctttga cagccgtttt
     6661 tagcctgagg gagagttttg atgagagtgt tcattttggg acttcgcgta ttctaggaaa
     6721 tagtatcggt ggactctatg ccttggtctt cttcttatta aatacttttt tccacgaagc
     6781 cttttgggtg accttggtag ttgttccaat ctgcaccatg ttaaccatta tgacaaatgt
     6841 agccatgaat aacaaagcag gggttattgg tggtgtagca gctatgttaa tcattaccct
     6901 atcaattcca agtggcgaga caattttgta cgtgtttgtg cgtgtattag aaacgtttat
     6961 gggagttttt gtcgcaatta tcgtaaatta cgatattgat cgtattcgtc tctttttaga
     7021 gaaaaaagaa aaataatgtt acattttata acattatcaa ttgacgtttg tcttttttta
     7081 gactataata gacagaaaga aggaaattgt aaatgaagga aaaagaattt cgccgaaata
     7141 tggctgtttt tcctatcggc agtgttatga agttgaccga tctatcggcg cgtcagattc
     7201 gttattatga agatcaagag ttgatcaagc ccgatcgaaa cgaaggaaat cgtcgcatgt
     7261 attccttgaa tgacatggat cgtctgcttg aaatcaaaga ttatatctct gaaggttata
     7321 atatcgctgc cattaagaaa aaatatgctg aacgtgaagc gaaatccaag aaagcggtta
     7381 gtcagactga agtacgtcgt gcacttcaca atgaactcct ccaacagggg cgttttgctt
     7441 cagtacagtc accttttggt cgcggttagg caaccgcaag tagtcataga ttaaggagaa
     7501 aacttatgcc aatcacagct gcagatattc gtcgtgaagt caaggaaaaa aatgttacct
     7561 ttattcgtct tatgttctca gatattttgg gaaccatgaa aaacgtcgaa attcctgcta
     7621 cagatgaaca gttagataag gtcttgtcga acaaggttat gtttgatgga tcttctattg
     7681 aaggttttgt acgtatcaat gagtcggata tgtacttgta tccggacttg gatacatgga
     7741 cagtcttccc ttggggagat gaaaatggaa gtgttgcagg tctgatctgt gatgtctata
     7801 caacagaagg tgaaccattt gcgggtgacc ctcgtggtaa tttgaaacga gctcttcgtc
     7861 acatggaaga agttggattc aaatccttca accttggtcc agagccagaa ttcttcctat
     7921 ttaagttgga tgaaaatggg gacccaacac ttgaagtgaa tgacaagggt ggctactttg
     7981 atttggcacc tactgacctt gcggacaaca cacgtcgtga gattgtgaat gtcttgacca
     8041 aaatgggatt tgaagtagaa gcgagtcacc acgaggttgc ggttggacag catgagattg
     8101 actttaagta cgatgaagtt ctccgtgctt gtgataagat tcaaatcttt aagcttgttg
     8161 ttaaaaccat tgctcgcaaa cacggacttt acgcaacatt tatggcgaag ccaaaatttg
     8221 gtattgctgg atcaggtatg cactgtaata tgtccttgtt tgatgcagaa ggaaataacg
     8281 ccttctttga tccaaatgat ccaaaaggaa tgcagttgtc agaaacagct taccatttcc
     8341 taggcggttt gatcaagcat gcttacaact atactgccat catgaaccca acagttaact
     8401 catacaaacg tttggttcca ggttatgaag cgcctgttta cattgcttgg gctggtcgta
     8461 accgttcgcc acttgtgcgc gtacctgctt cacgtggtat gggaactcgt cttgagttgc
     8521 gttcagtgga tccaatggcg aacccttacg ttgctatggc tgttcttttg gaagttggtt
     8581 tgtatggtat tgaaaataaa atcgaagcac cagctcctat cgaagaaaat atctacatca
     8641 tgacagcaga agagcgcaag gaagctggta ttacagacct tccatcaact cttcacaacg
     8701 ctttgaaagc tttgacagaa gatgaagtgg ttaaagctgc tctcggagat cacatctata
     8761 ctagcttcct tgaagccaaa cgaatcgaat gggcaagtta tgcaaccttc gtttcacaat
     8821 gggaaattga taattattta gacctttact aatactaata tagaagaaag attgcctgtg
     8881 ggtaaacagt cacacagcag tctaagccat caaataccta taacccttaa aatagcgatt
     8941 tcggtcgcta tttatttttc gaaaaatcct cttgactctt ttgcgaatta gatttatcat
     9001 cagacgtgta gaagaaaatt aaggagtcac tatggaaaaa ttcaatttca aaaacaatat
     9061 cgggcaagaa aacaagttac ttcaaatcga aatctacaag tttactaact tttacaagct
     9121 tcaaaattac acatctgtaa atattttttc gaaagatata tttgaagcca tagtaaattg
     9181 agactagaat agtacactac atctgctaaa aatttctaga aactaattta actttcctaa
     9241 tcgatttgtt tacatcttat ttcaatctac tatattatct caataaatga tgatggcgac
     9301 tgttttgttt gtgcttgtcg atttttgaat ccctgatcga gcggaagaat atttctgccg
     9361 aggttgccaa ggggaaaata ctgaagatat tgaattttat gatgaacaat tacaggcaga
     9421 aaaggtggag gttttgaata ttcgattgga agtaaaaaat tgaattatct acaatctcat
     9481 caacaataaa cctatttcaa ccccattatc tataggcgta tttttacgcc tttttatata
     9541 cttgaagcac ataagaaaca ttatgggaag tttgctgatg gaagtactca agaaattgat
     9601 gttccttatg atattcctga tacttgggag tgggtgagga taaaatcaat ttattggaat
     9661 tttgggcaaa ataagccaga gaaatccttt aggtatatag atacgtctag tattgataga
     9721 aaaaagaaca taatcaacta caaaaatcta caatatcttt cacctgaaca agcgccttcc
     9781 cgtgccagaa aattagtttc gcagaatagt gtcttatttt caacagttag accatatcta
     9841 aaaaatattg ctgtagttag agaacttaaa gagtatttga tagct
//
LOCUS       DAVMAF010000069         9796 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMAF010000069 DAVMAF010000000
VERSION     DAVMAF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.070"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(172..1935)
                     /locus_tag="VYO97_001684"
     CDS             complement(172..1935)
                     /locus_tag="VYO97_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1754447.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1944..2534)
                     /locus_tag="VYO97_001685"
     CDS             complement(1944..2534)
                     /locus_tag="VYO97_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1754448.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2632..3123)
                     /gene="rocS"
                     /locus_tag="VYO97_001686"
     CDS             complement(2632..3123)
                     /gene="rocS"
                     /locus_tag="VYO97_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1754449.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3200..3892)
                     /locus_tag="VYO97_001687"
     CDS             complement(3200..3892)
                     /locus_tag="VYO97_001687"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1754450.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            complement(3909..4223)
                     /locus_tag="VYO97_001688"
     CDS             complement(3909..4223)
                     /locus_tag="VYO97_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1754451.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4235..4780)
                     /locus_tag="VYO97_001689"
     CDS             complement(4235..4780)
                     /locus_tag="VYO97_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1754452.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4790..6169)
                     /gene="glmU"
                     /locus_tag="VYO97_001690"
     CDS             complement(4790..6169)
                     /gene="glmU"
                     /locus_tag="VYO97_001690"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1754453.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            6323..7123
                     /locus_tag="VYO97_001691"
     CDS             6323..7123
                     /locus_tag="VYO97_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1754454.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7218..8060
                     /locus_tag="VYO97_001692"
     CDS             7218..8060
                     /locus_tag="VYO97_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1754455.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGALAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLTWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            8060..8542
                     /locus_tag="VYO97_001693"
     CDS             8060..8542
                     /locus_tag="VYO97_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1754456.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8542..9162
                     /locus_tag="VYO97_001694"
     CDS             8542..9162
                     /locus_tag="VYO97_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1754457.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9416..9511)
                     /locus_tag="VYO97_001695"
                     /pseudo
     CDS             complement(9416..9511)
                     /locus_tag="VYO97_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2994 a         2081 c         1690 g         3031 t
ORIGIN      
        1 actatatttt ataagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga
       61 aacaagaaca atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc
      121 acaagaatac ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc
      181 aaagaattcc aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac
      241 ttctatttcc cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg
      301 aagagatttg ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt
      361 tgtccatagt ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt
      421 cgctcttgtt ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc
      481 atcatactgt cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata
      541 aatcttgagc tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt
      601 tttactggtt ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata
      661 atcttcaaag cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag
      721 ctcctccaag tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc
      781 atcatgcatc atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc
      841 tggtttgagt tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt
      901 ggaaacccct cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat
      961 ataaccttct aaggcaaaga aaagactggc acaagtcatg acaccccatc gcacgatgtg
     1021 atctttttct ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt
     1081 aaccaaaaaa taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat
     1141 accaagaatc agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa
     1201 agggatcaaa ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca
     1261 agcataaaaa gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac
     1321 agtcagctga tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag
     1381 aaagataaga gatagaagta ggatgctcag ccaccacatc caatatttat cttctaaata
     1441 agcttggata aactctggaa tgacgatttt attaagataa taaatcttca acattttccg
     1501 tataaaagga aaaagcatag ctatatagaa aaagataaac aaggctttag cgcaagttag
     1561 ctttttcata aatccaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg
     1621 cttttcatta tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc
     1681 aaccagaagg ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt
     1741 aaggatggct aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa
     1801 gctagccaac catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa
     1861 aaatagaaag aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg
     1921 tttttcaggt ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa
     1981 ggattgtttg ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag
     2041 aaggattcgt aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct
     2101 tgaaatccaa gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct
     2161 gaactccgac gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct
     2221 aaaccatgct ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc
     2281 aaagaaccca caaactcttg gaattcttct aagactttct caactggagg agcatctttt
     2341 aaggtctcag ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta
     2401 tgaacatagg aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact
     2461 tgaatcaagt gtgtttgtcc ttcgtgctga ttaaattcta aatcaaaagc aatgtaatct
     2521 tctaatcgtt ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt
     2581 cacaaacaaa agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc
     2641 aaataaacga gcaaaaaagc ctttcttagt ggattggact tcttcttttg cttggtccag
     2701 ctctagctta agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc
     2761 taattgtttg tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc
     2821 tgataactga cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt
     2881 ctcatccact agaatctcca tcagttcacg ttgtttgaca tcttcactga caggctcatc
     2941 ttcaaaaatc gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac
     3001 tgttacccct ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt
     3061 gattgcttgg cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact
     3121 cataatatcc tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga
     3181 aaaactgtca aatttccgct taatctaaag ccttcaaaaa ggtcaacaag acttgggcag
     3241 agcgacgtcc agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat
     3301 tgtcactcat agctctgatg actaagactg ggagattgag ggcatgcgct gcttgagcaa
     3361 tagctgcccc ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta
     3421 tcttgtcatt tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt
     3481 ggtccaattg agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat
     3541 aaagcggttg ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat
     3601 gataggctaa tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc
     3661 cagctgaacc cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac
     3721 tcatagcaga catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa
     3781 tggttcctgt atgataggta ttccccaaaa caacttgctc ctgggcatta tctaaatgct
     3841 ggaccagata agccagttct tctggcatag cagcaataat tcctattttc atttcaattc
     3901 cttttctatt acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgca
     3961 aataagattt tattcaactt agaattgaag acatttctct tggtattttc aatacgacgg
     4021 cttttatgaa tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgatcc
     4081 ttaggattgg cataaccaaa acgggaagaa gaggttggta aaatcttagt ttcctcatta
     4141 tcatctagca aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca
     4201 tccgttaata aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact
     4261 gaacagccat aattgtcttg gcatcacaaa tatgacctga ttggattaat tctttcgctt
     4321 cttctaagct cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt
     4381 ccacttttgt caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag
     4441 ctgaataaaa atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt
     4501 cacgaagggc agctgccaca ggggctgtgt tttctcctac ttccaatttt ccggctggaa
     4561 tttcgtaaga gacagcctcg atagctttgc ggtactgctt gaccaagata agtttttgtt
     4621 catccgttac tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag
     4681 ttcccttgcc ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat
     4741 agatttcttt tcggctaagc gttttttctt caaactccat gataggctcc tactggttct
     4801 taggatgatg aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa
     4861 tagcaatagc atctgctggc acgtctttag taatagttga accagcacca acgagggaat
     4921 tgtcaccaag ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc
     4981 caatgactgt cttgtatttg tttttgccgt catagttgac tgtaatagtt ccagcaccga
     5041 aattaacgtt gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat
     5101 tctcaccgat tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca
     5161 gacttgaatt tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac
     5221 tttcctcaat catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat
     5281 aagtaccgtt tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat
     5341 tggcttcgat ttgaacttcc gaagcaatct caacatcaat atcgatataa gttgcttctg
     5401 gattgacaaa gctaacaccg ttgaccatgt gtttatgatt gatgcgacga cgcataactg
     5461 actcagctgt cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca
     5521 aagtataagc gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat
     5581 agtattcgcc ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt
     5641 tgtcaaagac gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat
     5701 ccttctgctc aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc
     5761 caaaaggatt atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga
     5821 aatcaatcaa gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca
     5881 aggtgtgtcc tgacaaacct tctaagatag gctctgtcat cataactgca tgaccagttc
     5941 ccaactgttc agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt
     6001 ctgccttgtg tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga
     6061 aaacatgttc caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt
     6121 tcatgcgagt ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt
     6181 ttctgtagaa attccctttt attataccat atttcaaatc attccgcact cttgaatgaa
     6241 aaaatgctct aaagtctctt ccttaaccca tttttttgat tttttttgat tttttttgat
     6301 ttttagggta gaattataag atatgagaaa acaaattcat ccacttattt tatttagttt
     6361 tttggggatt atgatgttca ttttaatcat caatcgccca cttaatgaca accaatcgtt
     6421 taaaacaaaa tccaatatag cacagattga agcacaggct ttgaaacact tagacaaacc
     6481 gattattgac ctctctggct ggcagcgtcc tgaagaaatc aactacgatg ccctctcaca
     6541 aaatatttca ggtgctattg ttcgtgttca cagtggtgct caaacgacaa aagaaaatga
     6601 tgcttcattt atcaatggaa tagacaaggc ctataagagt catatcactg aacttcaaaa
     6661 gcggaatgtc ccagttgctg tctacgctta tgtagctgga aaaagtgtcc aagaaatgga
     6721 aaaggctgcc gaagttttct acaatgctgc ctctccttac agccctagtt actattggct
     6781 tgacgtggaa gacaaaacca tgtctaatat gaacgaaggt gtcgaaaact ttcgagcaaa
     6841 actagcatca ctaggtgcta aaaacatcgg catctacgtt ggtgtctact tcatggaaga
     6901 acacagtatt gatacaggca agtttacctc tgtttggatt ccgtcctacg gctcagactc
     6961 aggatttttg gagagcagtc ctaaaactga tttagattat gatattcacc aatacacatc
     7021 taaaggaaaa attgccggct ttgaccacga tttagatatc aacgttatct ctcccttaaa
     7081 aaataaagaa gaaacctttc gaaaactttt tttaaaacct taaaagcatt tgtttatcat
     7141 ttgctttttc tttttgtgtc tgattgtgat acaatatagt aaggattttt tgaaatagaa
     7201 atagttggag gaaatatatg ctctcatggt tagcacgcgt tattaaaggg attgtaattg
     7261 ctcttggatt tatcctaccg ggaatttccg gaggggctct agcagcaatc ttaggaatct
     7321 atgaacgaat gattggcttt ctggcccatc cctttaaaga ctttaaagaa aatgttttgt
     7381 actttattcc agttgccatc ggtatgcttc taggaatcgg cttattttcc tacccgattg
     7441 aatacctgct tgaaaattat caggtctttg tattatggag ctttgcggga gctattatcg
     7501 gtacagttcc tagcctcctc aaagaatcaa ctcgagaatc tgaccgagac aagattgatt
     7561 taacttggtt atggacaacc tttatcattt ctggattagg actctatgcc ttaaattttg
     7621 tcgttggaac cttaagcgcc agctttctta acttcgtcct agcaggcgca ctattggccc
     7681 ttggcgtctt ggttcctggc ctcagcccat caaatttact tttgattttg ggactctatg
     7741 ctcctatgtt gactggtttt aaaacttttg atctcttggg aaccttcttt ccgattggaa
     7801 ttggtgcagg tgcaactctc atcgtttttt caaaattgat ggattatgcc ttaaacaact
     7861 accactcacg cgtctatcat ttcatcatcg gtatcgtcct atcaagtacc cttttgatct
     7921 taattccaaa tgcaggaaac gctgaaagta tccaatacac aggactttca cttgtcggtt
     7981 atgtcatcat cgccttcttc tttgcgctgg gaatctggct tggtatttgg atgagtcaat
     8041 tggaggataa atataaataa tggcaaaaaa agttaaaatc aaaaaaacat tggtggaaca
     8101 aatcctatct aaagcagcta tccctcatca gggaattcaa atcaatgccc tagaaggaga
     8161 gcttcctcaa ggttatgaac gagatcagat tttcaaaacc ttggcgcttt tgggagacaa
     8221 gaccggaccg attatcggaa ttgtccctat cactcagcac ttgtctgaaa agaaactagc
     8281 taaaatttct ggcaataaaa aagtgagcat gattccacaa aaggacttag aaaaaacaac
     8341 tggttacatt catggagcca ataatcctgt cggaattcgt cagaaacaca attatcccat
     8401 ttttatcgat aaaattgctc tagatttgga tcaaatgatt gtctctgcag gcgaagtcgg
     8461 acacagcatt atcgtcgcgc cacaagactt ggctagcttt gtaaaggcag actttgtaga
     8521 tattttggag gacatcaagt aatgaaactc tactttgtcc gccacggtcg taccctctgg
     8581 aaccaagaag gtcgttttca aggtgctagt ggtgattctc ctcttcttcc tgaatccatt
     8641 gaaaccctaa aacgacttgg tcagtatctc aaggaaattc cttttgatca gatttattca
     8701 agtgatttac ctcgagcggt caaatctgct gagattatcc aaagtcaact ctataccccc
     8761 tgttctttag aaatcgttcc taatctccgt gaatggcaac ttgggaagtt agaaggtttg
     8821 aaaattgcaa ccttggaagc tatttacccg caacaaatcc aggcttttcg ttcaaatctt
     8881 gctcaatttg acactcgaat gttcggagcc gaatccctct atagtacaac tcaacggacc
     8941 atccaattta tcaaatcatt aaaagatagt ccagctgaac gtattctaat tgtcggacac
     9001 ggcgccaatc ttactgccag tcttcgtact ctcctaggct ataaagaacc acttcttcgt
     9061 aaagatggcg gtctagcaaa tgccagcctg accattatag aaacccatga ttttgaaaca
     9121 ttcactctca atacttggaa tgatacttct tatcaatcat aattatagcc tagccttctg
     9181 aactagaacc ataggagata tagtcagttg aaacaaaaat aagataaaag agcctcgtaa
     9241 aaaggtattg caacttgcca ataccttttt tgatatgaac acgtgtcttc tcaacaggat
     9301 tgtagagcaa aatgaaataa gaactgaata atatgatttg agaatttaaa tcaatttata
     9361 agaatgtttt agaagcagtt atgtactatt ttagattcac ttttctatac taagatgagt
     9421 agggaggaaa aggtaaaagt ttatgcccaa actcttcaca taagaactct agcttaccca
     9481 ttctatggaa tcttgcatta tccattaaaa cttccagact catacccttc taaaatctat
     9541 tcaaaccacg tcagcttcac attatatatg ttggttacct cacaagtttt atctgcaacc
     9601 tcaaagctgt tctttgagca gcctacagct agcttcctag tttaatcttt gcttttcact
     9661 gagtatcaag ttaaaaaatg aggctggtca aaaagtcttt aaaataaaaa aaacgcatat
     9721 tatcaggtgt taaatgaact gcaccccaaa agttagacag aaaaaatcta acttttggag
     9781 tgtttttatt atgaaa
//
LOCUS       DAVMAF010000070         9631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMAF010000070 DAVMAF010000000
VERSION     DAVMAF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.071"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            26..5917
                     /gene="iga"
                     /locus_tag="VYO97_001696"
     CDS             26..5917
                     /gene="iga"
                     /locus_tag="VYO97_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HEV1754458.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLTEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDSYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     SPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPAVKLTELY
                     LEEQFSKTKQNLGGSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     HHGTTDLFSALETYRKVFLPNTSNNDWFKKQTKAFIVEEKSTIAEVKAKQKQAGTKYS
                     IGVYDRITSNTWKYRNMVLPLLTLPERSVFVISTLSSLGFGAYDRYRNSEHKAGKDLN
                     NFVEENASETAKRQRDHYDYWYRILDNEGREKLYRTILLYDAYKFGDDTTSGKATVEA
                     KFDSSNPAMKNFFGPVGNKVVHNHHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRSGLGPEFFAKGLLQAPDHPYDATITINSILKHSKSDSLEGSRLQVL
                     DPTERFQNSADLQNYVHNMFDLIYMLEYLEGQSIVKKLNVSQKMEALRKIENQYLTDP
                     ADGNEVYATNVVKELTEEEARNLNSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAP
                     IFSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEGIAKQNGQTINLYGK
                     KQGLVTDKLVLEKIFSGKYPSWTAFKKAMYQERIAQFDHLTKVIFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYNPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            5969..6172
                     /locus_tag="VYO97_001697"
     CDS             5969..6172
                     /locus_tag="VYO97_001697"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1754459.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVRNSFLLPLKSHHLMAFFYIG"
     gene            6365..7216
                     /gene="ylqF"
                     /locus_tag="VYO97_001698"
     CDS             6365..7216
                     /gene="ylqF"
                     /locus_tag="VYO97_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1754460.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7203..7982
                     /locus_tag="VYO97_001699"
     CDS             7203..7982
                     /locus_tag="VYO97_001699"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1754461.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7998..9548
                     /locus_tag="VYO97_001700"
     CDS             7998..9548
                     /locus_tag="VYO97_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1754462.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMSDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQILGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVRNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3244 a         1782 c         1969 g         2636 t
ORIGIN      
        1 ataaatttta tttcaaggag gaataatgga aaagtatttt ggtgaaaaac aagagcgttt
       61 ttcatttaga aaattatcag taggacttgt atctgcaacg atttcaagtt tattttttat
      121 gtctgtatta gctagttcat ctgtggatgc tcaagaaact gcgggagttc actataaata
      181 tgtggcagat tcagagctat catcagaaga aaagaagcag cttgtctatg atattccgac
      241 atacgtggag aatgatgatg aaacttatta tcttgtttat aagttaaatt ctcaaaatca
      301 actggcggaa ttaccaaata ctggaagcaa gaatgagagg caagccctag ttgctggtgc
      361 tagcttagct gctctgggaa ttttaatttt tgctgtttcc aagaaaaagg ttaagaataa
      421 aacggtatta catttagtat tggttgcggg aatgggaaat ggtgtcttag tttcagtcca
      481 tgctttagaa aatcatcttt tgctaaatta caatacggac tatgaattga cctctggaga
      541 aaaattacct cttcctaaag agatttcagg ttacacttat attggatata tcaaagaggg
      601 aaaaacgact tctgattttg aagtaagtaa tcaagaaaaa tcagcagcca ctcctacaaa
      661 acaacaaaag gtggattata atgttacacc aaattttgta gaccatccat caacagtaca
      721 agctattcag gaacaaacac ctgtttcttc aactaagccg acagaagttc aagtagttga
      781 aaaacctttc tctactgaat taatcaatcc aagaaaagaa gagaaacaat cttcagattc
      841 tcaagaacaa ttagccgaac ataagaatct agaaacgaag aaagaggaga agatttctcc
      901 aaaagaaaag actggggtaa atacattaaa tccacaggat gaagttttat caggtcaatt
      961 gaacaaacct gaactcttat atcgtgagga aactatagag acaaaaatag attttcaaga
     1021 agaaattcaa gaaaatcctg atttagctga aggaactgta agagtaaaac aagaaggtaa
     1081 attaggtaag aaagttgaaa tcgtcagaat attctctgta aacaaggaag aagtttcgcg
     1141 agaaattgtt tcaacttcaa cgactgcgcc tagtccaaga atagtcgaaa aaggtactaa
     1201 aaaaactcaa gttataaagg aacaacctga gactggtgta gaacataagg acgtacagtc
     1261 tggagctatt gttgaacccg caattcagcc tgagttgccc gaagctgtag taagtgataa
     1321 aggcgaacca gaagttcaac cgacattacc cgaagcagtt gtgaccgaca aaggtgagac
     1381 tgaggttcaa ccagagtcgc cagatactgt ggtaagtgat aaaggtgaac cagagcaggt
     1441 agccccattg acagaatata agggtaatat tgagcaagta aaacctgaaa ctccggttga
     1501 gaagaccaaa gaacaaggtc cagaaaaaac tgaagaagtt ccagtaaaac caacagaaga
     1561 aacaccagta aatccaaatg aaggtactac agaaggaacc tcaattcaag aagcagaaaa
     1621 tccagttcaa cctgcagaag aatcaacaac gaattcagag aaagtatcac cagatacatc
     1681 tagcgaaaat actggggaag tgtccagtaa tcctagtgat tcgacaacct cagttggaga
     1741 atcaaataaa ccagaacata atgactctaa aaatgaaaat tcagaaaaaa ctgtagaaga
     1801 agttccagta aatccaaatg aaggcacagt agaaggtacc tcaaatcaag aaacagaaaa
     1861 accagttcaa cctgcagaag aaacacaaac aaactctggg aaaatagcta acgaaaatac
     1921 tggagaagta tccaataaac ctagtgattc aaaaccacca gttgaagaat caaatcaacc
     1981 agaaaaaaac ggaactgcaa caaaaccaga aaattcaggt aatacaacat cagagaatgg
     2041 acaaacagaa ccagagaaaa aactcgaatt aagaaatgtt tctgatattg agttgtatag
     2101 tcagacgaat ggaacctaca gacaacatgt ttcattggat ggaattccag agaatacgga
     2161 tacttacttt gtcaaggtaa aatcttcagc atttaaagat gtctatatac cagtagcttc
     2221 aataaccgaa gagaaaagaa atgggcagtc agtttataaa atcacagcca aagctgagaa
     2281 actccagcaa gaactagaaa ataaatatgt cgacaatttc accttctacc tcgataagaa
     2341 ggctaaagag gaaaatacaa actttacttc ctttagcaac ctggtcaaag ctataaacca
     2401 aaatccctct ggaacctatc atttagcggc cagcctgaat gctaacgaag tggagcttgg
     2461 tcctgatgaa agatcctata tcaaggacac ctttactggt cgtttaatcg gtgaaaaaga
     2521 tggcaagaat tatgctatct ataatttgaa aaaacctctg tttgaaaact tgagtggtgc
     2581 tacagtagaa aaactgagtc taaaaaatgt tgctatttca gggaaaaatg atattggttc
     2641 actggcaaat gaagctacga atggcacaaa gattaaacaa gttcatgttg atggtgttct
     2701 ggctggagaa cgtggtgtcg gtggtttgtt ggctaaggca gaccaatcaa gcatcgcaga
     2761 gagcagtttc aagggaagaa ttgtcaatac ctatgaaacg actgattcct acaatattgg
     2821 cggtctggtc ggtcatttaa caggaaaaaa tgcgtctatt gctaaatcca aagcgacagt
     2881 aaccatttcg tcaaacacca ataggtcaga tcagactgtc ggtggtcttg caggtctagt
     2941 agaccgagat gcacagatcc aagatagcta tgctgaaggt gatatcaaca atgtcaagca
     3001 ctttggtaga gtcgctggag tggcaggcaa tttgtgggat cgaacttctg gtgatgttag
     3061 gcatgctgga agtttgacaa atgttctcag cgatgttaat gtaacaaacg gaaatgccat
     3121 cactggatac cactatacag gaatgaaggt agctaataca tttagtagta aggctaatag
     3181 agttttcaat gtcactttag agaaggatga ggtcgtcagc aaggaatcct ttgaagaaag
     3241 aggaacaatg ctagatgctt ctcaaattgt aagcaaaaaa gcagaaataa gtcctctcac
     3301 tctaccaacg gtggaacccc tctcaacaag tggcaagaaa gacagtgatt tttctaagat
     3361 agcccattat caagctaacc gtgctttggt ttataagaac attgaaaaat tgttaccttt
     3421 ttataataag tcaaccatcg tgaaatacgg aaacctggtt aaggagaaca gtctcttata
     3481 ccaaaaagaa ctcttatctg cagttatgat gaaggatgac caagtaatca cagatattgt
     3541 ttccaacaaa cagactgcaa acaaactctt acttcactat aatgaccatt catctgagaa
     3601 atttgatctc aagtaccaga ctgattttgc caatctagca gaatataatc taggtaatac
     3661 gggacttctc tacactccta accaattctt atatgatcga gactctattg ttaaggaagt
     3721 cttgcctgag ttgcagaagc ttgattacca gtcagatgct atcagaaaga cacttggtat
     3781 ttctccagca gttaagttaa ccgagctcta tttagaagag cagttctcca aaacaaaaca
     3841 aaatctggga ggcagcttga aaaaactttt gtcagcagat gccggtctag ctagcgataa
     3901 ctcagtcacc agaggctatc ttgtagataa aatcaagaat aataaggaag ccttgctact
     3961 cggtttaact tatttagaac gttggtataa ctttaactat ggtcaagtga atgtcaaaga
     4021 cctagttatg tatcatccag acttctttgg taaaggaaat acttccccac tagatactct
     4081 gattgagtta ggtaaatctg gctttaacaa tcttcttgcc aagaacaatg tcgatactta
     4141 tggtatcagt ctagccagcc atcatggaac gacagatttg tttagcgcac ttgaaactta
     4201 ccgaaaagtc tttttaccaa acactagcaa caatgactgg tttaaaaaac agaccaaagc
     4261 tttcattgtc gaagaaaaat ccactattgc agaggtcaaa gccaagcaaa agcaagcagg
     4321 aactaagtac tctatcggtg tttatgaccg aattactagt aatacttgga agtaccggaa
     4381 tatggtcttg cctctcctta ccttgccaga aagatctgta tttgtcatct caaccttgtc
     4441 tagtctagga tttggtgctt acgatcgcta ccgtaatagt gaacataaag caggcaagga
     4501 cctcaataat ttcgttgaag aaaatgcgag tgaaactgcc aaacgtcagc gagatcacta
     4561 cgattattgg tatcgcattt tagataatga gggacgtgaa aaactatatc gtacgattct
     4621 cctttatgat gcctataagt ttggtgatga cacaacatct ggaaaagcta cagtggaggc
     4681 taagtttgat agctccaatc cagccatgaa gaatttcttt ggtccagttg gtaataaggt
     4741 tgttcataat catcacggtg cctacgcaac aggagatggt gtctactata tgtcttaccg
     4801 catgctcgac aaggatggag ccattaccta tacccatgag atgacccatg attcggatca
     4861 agatatttac cttggcggct atggtcgaag aagtggtctt ggtcctgagt tctttgctaa
     4921 aggtttatta caagcgccag accatccata tgatgcgacc attaccatta actctatctt
     4981 gaaacattca aaatcagata gcttagaagg atcgcgtctg caagtcttgg atccgacaga
     5041 aagattccaa aactcagcag atcttcaaaa ctatgttcac aacatgtttg acctcatcta
     5101 tatgttggaa tatctagaag ggcaatcgat tgttaagaaa ctgaatgttt ctcagaaaat
     5161 ggaagctctc agaaaaatcg agaatcaata tctaacagat cctgctgatg gaaatgaggt
     5221 ttacgctact aacgtagtca aagaattgac agaagaagag gcccgaaacc tgaatagttt
     5281 tgatagtttg attgacaata atattctttc agctcgtgag tataaggctg gtacatatga
     5341 gagaaatggc tactttacta taaaactctt tgcaccaatc ttttcagcat tgagcagtga
     5401 gaaaggtact cctggagatc ttatgggacg aaggatagct tatgaactct tagctgctaa
     5461 aggatttaaa gatggtatgg taccatatat ttccaaccaa tatgaaggaa ttgctaaaca
     5521 aaatggtcag acaatcaacc tttacggtaa aaagcaagga ttagtgacag ataagcttgt
     5581 tttggaaaag atattctctg gcaagtatcc atcttggaca gcctttaaaa aagctatgta
     5641 tcaagagcgt attgctcagt ttgatcattt gactaaagtt atttttaaag atccgacaaa
     5701 accatggcca agctatggca caaagactat caataatgtg gatgaattgc aagccctcat
     5761 ggaccaagct gttctcaagg atgctgaagg tccacgttgg agtaattata atccagaaat
     5821 cgacagtgcc gttcataagt tgaagagagc aatctttaaa gcctatcttg accaaacaaa
     5881 tgattttaga agttcaattt ttgagaataa aaaatagtgt ttactattag gaaataaagt
     5941 ttaaaaaggt gatgaagaac aaaccaagat tcaagcagga attcctactg ataatgaagt
     6001 aagttatgct cttatttatc agcaggaaac tcttcctgca acaggttcat caacttctgc
     6061 gcttacagct ttaggcctat tagctgttgg tagtttagtt cttttggttc ggaacagttt
     6121 tttgctccct ctgaaaagtc atcatttgat ggcttttttc tatataggat agggtatagg
     6181 gtaaaagata gggtaaaagg ctatcatcgg acaaaataaa gaaggcatga tataatataa
     6241 agtagatttc tatgtcataa aacaagaact gtttggacat cattcatttg aaaactctct
     6301 atgttcaaac tatagtaaaa taaaataggg gatctaaatc cttgctatga aaggaaaaaa
     6361 ctcaatggct actattcaat ggtttcctgg tcacatgtct aaagctcgtc gacaggtgca
     6421 ggagaattta aaatttgttg attttgtgac gattttagta gatgcacgct tgcctctatc
     6481 tagtcaaaat cctatgttga ccaagattgt tggtgataaa ccaaaactct tgattttaaa
     6541 caaggccgac ttggctgatc cagcaatgac caaggaatgg cgtcagtatt ttgaatcaca
     6601 aggaatccag acgctagcta tcaactccaa agagcaagtg actgtaaaag ttgtaacaga
     6661 tgcggccaag aagctcatgg ctgataagat tgctcgccag aaagaacgtg ggattcagat
     6721 tgaaaccttg cgtaccatga ttatcgggat tccaaacgct ggtaaatcca ctctgatgaa
     6781 ccgtttggct ggtaaaaaga ttgctgttgt tggaaacaag ccaggggtca caaaaggtca
     6841 acaatggctt aaaaccaata aagatctgga aatcttggat acaccgggga ttctctggcc
     6901 taagtttgag gatgaaactg ttgcacttaa gttggcattg actggagcta tcaaggatca
     6961 gttgcttcct atggatgagg ttaccatttt tggtatcaat tatttcaaag aacattatcc
     7021 agaaaagctg gctgaacgct tcaaacaaat gaaaattgaa gaagaagcgc ctgtgattat
     7081 tatggatatg acccgcgccc tcggtttccg tgatgactat gaccgttttt acagtctctt
     7141 cgtgaaggaa gtccgtgatg gcaaactcgg taactatacc ttagatacat tggaagacct
     7201 cgatggcaac gattaaagaa atcaaagaac tccttgtgac agtcaaggag ttagaaagcc
     7261 ctattttttt agatcttgaa aaggataatc gctcaggagt tcaaaaggaa atcagcaagc
     7321 gtaaaagagc cattcaagct gaattagatg aaaatttgcg cttggaatcc atgctttctt
     7381 atgaaaaaga actttataag caaggattga ccttaattgc aggtattgat gaggttggtc
     7441 gtggtcctct tgctggtcct gtagtcgctg cggccgttat tttacctaaa aattgtaaga
     7501 ttaaaggtct caacgacagc aagaaaattc ctaaaaagaa acatctggag attttccaag
     7561 ccgttcaaga ccaagccttg tcgattggaa ttggtatcat agataatcag gtcatcgacc
     7621 aagtcaacat ctatgaagca accaaactag ccatgcaaga agcaatctcc cagctcagcc
     7681 ctcaaccaga gcaccttttg attgatgcca tgaaactgga cttgcccatt tcacaaacct
     7741 ccattatcaa aggagatgcc aactccctct ctatcgcagc agcatctata gtagccaagg
     7801 taacacgtga tgaattgatg aaagaatacg atcagcagtt ctctggctat gatttcgcta
     7861 ctaatgcagg atatggcaca gctaaacatc tggaaggcct cacaaaacta ggagttaccc
     7921 caattcaccg aaccagcttt gaacccgtta aatcactggt tttaggtaaa aaagaaagtt
     7981 aattgaaagg aaataccatg gaggaacagt cggaaatagt ccgttctaag aaagaattcg
     8041 cctttgcatc cagcactata ctatcccaag ttggtcgagg aatcattgtc ggcctcatcg
     8101 ttggaattat cgtcggatcc tttcgtttct taattgaaaa gggcttccac ctgatacaag
     8161 gagtttatca agatcaaggg tacttagtgc gcaatctttt tgtactggtt ttgttttata
     8221 tactcatctg ttggctcagt gccaaactaa cacggtcaga aaaagatatt aaaggctcag
     8281 gaattcctca agtcgaagcc gaactgaaag gcctcatgtc cctcaactgg tggggcattc
     8341 tttggaaaaa atatgtgcta ggtattcttg ctattgccag tggactcatg ctgggtcgag
     8401 agggacccag cattcaactt ggagcagttg gtggtaaagg aattgccaag tggctcaaat
     8461 ccagtccagt agaggaacgt tccttgattg ccagtggagc tgcagcaggt ttagccgcag
     8521 cctttaatgc tcctattgca gcacttctct ttgttgtaga agaagtctat caccattttt
     8581 cgcgcttttt ctgggtctca actctagcag ccagcatcgt agcaaacttt gtgtctctac
     8641 tcatgttcgg tttgacacca gtattggata tgtcagataa cattcctccc atgaccctag
     8701 accagtattg gatatatctc gtcatgggaa ttttccttgg attttcaggt tttctctatg
     8761 agaaagctgt attaaacgtt ggaagagttt atgacttgat tggtcaaaaa atccatttgg
     8821 atagggctta ttatcccatc ttggctttta tccttatcat accagtcgga atcttcttac
     8881 ctcaaatcct tggtggcgga aatcagcttg tcctttcttt aactgaacaa aattttagtt
     8941 tccaagtttt attagcttac tttttgatcc gctttatttg gagtatgatt agctatggaa
     9001 gtggactgcc aggaggaatt ttcctaccca ttttagctct tggttctttg cttggtgcct
     9061 tagttggtgt tatctgtgtc aatcttggac ttgtcagtca agagcaattc cctatatttg
     9121 tcattctagg aatgagtggc tattttggag ccatatcaaa agctccctta accgctatga
     9181 tcctcgtaac tgagatggta ggagatattc gcaacctcat gccacttggt cttgtcactc
     9241 ttgtttctta tattatcatg gatttgctca aaggtacgcc agtctatgaa gccatgctgg
     9301 aaaaaatgct tccagaagaa gtatctagcg aaggagaagt tacacttatc gaaataccag
     9361 tttctgataa aattgctggg aaacaagttc atgaactcaa cttaccacac aacgtcctca
     9421 tcacaactca agtccgtaat ggcaagagcc aaacagttaa cggctcaacc agaatgtatc
     9481 tgggtgatat gattcacctg gttattccaa aaagtgaaat tggaaaagtc aaagatttgt
     9541 tgttgtagta tgatcattca cataatttat gttatgtaaa tgatcagttt gatttattta
     9601 gaaaaccgat tctcagaaat gagatcggtt a
//
LOCUS       DAVMAF010000071         9236 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMAF010000071 DAVMAF010000000
VERSION     DAVMAF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.072"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            129..365
                     /locus_tag="VYO97_001701"
     CDS             129..365
                     /locus_tag="VYO97_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1754463.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            362..775
                     /locus_tag="VYO97_001702"
     CDS             362..775
                     /locus_tag="VYO97_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1754464.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            893..1858
                     /locus_tag="VYO97_001703"
     CDS             893..1858
                     /locus_tag="VYO97_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1754465.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1881..2462
                     /locus_tag="VYO97_001704"
     CDS             1881..2462
                     /locus_tag="VYO97_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1754466.1"
                     /translation="MFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTK
                     NVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRI
                     NSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQ
                     NEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            2491..5841
                     /locus_tag="VYO97_001705"
     CDS             2491..5841
                     /locus_tag="VYO97_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1754467.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6347..6817)
                     /locus_tag="VYO97_001706"
     CDS             complement(6347..6817)
                     /locus_tag="VYO97_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1754468.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(6834..9107)
                     /locus_tag="VYO97_001707"
     CDS             complement(6834..9107)
                     /locus_tag="VYO97_001707"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1754469.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYDLTVDLAQSMLTLPC"
BASE COUNT         2979 a         1509 c         1937 g         2811 t
ORIGIN      
        1 gcaatccaaa aatctctgga agaacttgaa actttgaaga aatctctgat gcaggagtat
       61 tttggctgat attctgccat tgtaattacg gtaatgattt gttataatac ttcaaaggag
      121 gaaatcagat ggtagtaaaa acaagaaaac aaggaaattc aatcaccatt acgattccaa
      181 gtgaatttaa tattccaagt ggtgttaaat acgaagcgaa attgttacca agtggtgaga
      241 ttatctttac tcctgaagaa ttggggcagc aggtttctta tgtatctgat gatgcctttg
      301 acttaaattt agataaaata tttgacgaat acgacgatgt tttcaaagct ttggtggaaa
      361 aatgacaatc tatttgacag aaaagcaaat tgaaaaaata aatgctttag caattcaacg
      421 gtattctcca aatgagaaaa ttcaaacagt tagtccttct gccttaaata tgattgtgaa
      481 cttaccagaa caatttgtct ttgggaagcc tctttatcca acaatttttg ataaagcaac
      541 gatactattt gtccaattga taaagaagca tgtttttgct aatgctaata aaagaactgc
      601 tttcttcgtt ttggtcaaat ttttacaatt aaacggctat cgtttttctg taacggtaga
      661 agaagcagta aaaatgtgtg taaccatcgc agtagaagct ttaactgatg aaaaaatgac
      721 aagctactcc aaatggattt ctgaacattc tgttagagaa aaggtcaaaa agtaacctag
      781 tatgctggat ttgaatgagc acaagaaaat aaatgaacag acaatattag aattctgtaa
      841 tgcagaaact gatattgtct ctttttattg atgaataaga aagtgagaaa ttatggaatc
      901 aaaagttaca attatcatgc aagaaatgtt acctctttta aataatgaac aattactagc
      961 gttgagagag agtttagaac atcatctagt agacggaaaa aagcagcaga agtattcgaa
     1021 taataacctg ttgcaactat ttattaccgc caagcaggta gagggctgta gctcaaaaac
     1081 aattcgttat taccagagga cgattgaaaa cttgtttaat gctattaaag agtctgtgac
     1141 acaactcaca acagatgatt taaggagtta tttagcaaat taccagtctg aaaaggattg
     1201 tagtaaggca aatttagaca atattaggcg tatattgtct tctttttttg cttggcttga
     1261 gcaagaggaa tatatcatta aaaatcccat tcgacggata aagaaaatta agactgagca
     1321 aaatgtgaag gaaacttata ctgatgaaca tttggaaatt atgcgtgata actgtgaaaa
     1381 tttgagagat ttggcaataa tagacctact agcatcgaca ggtatgcgtg taggggagct
     1441 tgtacagttg aatcgttcag atattgattt tgaaaacaga gagtgtgttg tctttggtaa
     1501 aggaaagaag gagagaccag tatattttga cgctcgtacg aaaattcatt taagaaacta
     1561 tcttaacgac agaaaagata gtcaccctgc tctttttgta acgttagttg gaaaagtcca
     1621 gaggcttgga attgctggtg tagagattcg cttaagaaag ttaggagaca aactcggtat
     1681 acaaaaggtt cacccacata agttcagaag aactttagcg actaaggcaa ttgataaagg
     1741 tatgcctatc gaacaagtcc aaaaactgct aggtcatagc aagattgaca caaccctggc
     1801 ctatgccatg gtcaatcaaa ataatgtcaa gcattcacac caaaaattca tctcttaaaa
     1861 gcaaatcccg atttaacgag atgtttgggg aaaataaaat atttgaaagc attgataact
     1921 tatttgatat tatagatggt gataggggca aaaattatcc taaatcagat gagttgttta
     1981 gtgaggagta ctgtttattt ttaaatacaa agaatgttac taaaaacgga ttttcattcg
     2041 atacaaagca atttatcact aaaacaaagg ataaattact tcgaaaaggc aaacttgagc
     2101 gttatgatat agtcttgaca acaagaggta ctgttggaaa tgtagcgtac tacgatgaat
     2161 taataaaata taaacattta cgtataaatt caggtatggt aatattacgt cccaagacac
     2221 caaatctaaa tcagaaattt attatccatg ttttaaggaa taataattat agtcgagtga
     2281 tatcaggaag tgctcagcct cagttaccaa ttacaaaatt aaaaaaaata cttctccccc
     2341 tccccccact agccctccaa aatgagttcg cagactttgt agtccaggtc gacaaatcac
     2401 aatttgcttg tgagatagct ataaaagtgt ggagaaatag cttgaaattt agtataatat
     2461 agctaaacta tttgttaaag tgagaaaaaa atgggaaatt ttagctttct tttaaaaaat
     2521 gacgaatatg aatctttttc aaaaccttgc attgaagctg agaatatgat tgctacatca
     2581 actgtggcta ctgcctttat ggcgcgtcgt gctttagagc aggctgtcca ttggatatat
     2641 agtcacgatt catatttaga agctccctat cgtgctactc tatcttcttt agtatgggat
     2701 gatgatttta gggatatcgt agattctgaa ctccacaagc agatagttct gttgattcgg
     2761 tggggaaacc atgctgctca tggtggtgaa attaaggaac gagaagcgat tttagctttg
     2821 catcatttgt atcagtttgt taattttatc gattattgtt acagcaatga gtttgtggag
     2881 cgttattttg atgagaagtg cttaccactt tcagcaaaca tcaaataccg agaaactcca
     2941 caatctatga taaagttaca agacagttta ccagaactgc ctgattttca tgaacagatg
     3001 gctgctcagt ccgtagaagt tcaagagact tatactgaaa aacgtgagac tgcagcgcaa
     3061 cggcaagatg tgcctttcca tattgatcaa ttatctgagg cagagacaag aaagctcttt
     3121 attgatatcg atctccgttt agcaggatgg atatttgaag aaaactgtcg tgttgagata
     3181 gccgttgatg gtctcaagca cggttcagga attggttact gtgactatgt actttatggt
     3241 aaaaatggga aaattttagc gattgtggag gctaaaaaag cctctgtcaa tccagaagta
     3301 ggggaagtac aggtcaaaga atatgctgaa gctttggaga aacatatcgg ctatcagcca
     3361 atttgcttta ttacaaatgg gttgaagcac tatatacttg atggtccgaa ccgccgccag
     3421 attgcaggct tttactctca agaagaattg caattagtga tggatagacg tcatcttcaa
     3481 aaaccgcttg aggatatttc tagtaaaatt agggacgata tttccgggcg tcactaccaa
     3541 aaacatgcca ttgcaagcgt ttgtgaagct ttctctaatc atcgtagaca ggcacttttg
     3601 gttatggcaa ctggggcggg gaaaactcgt acagcagttt ctctagttga tatcttatca
     3661 cgtcataact gggtaaaaaa cgttctcttc ttagccgata gaacttcctt ggttaagcaa
     3721 gcctatgatt cgtttagaaa attactccca gatctttccg tttgtaactt cttagaagat
     3781 aaagaaggag ctcaatcaag tcgtatggtc ttttcaactt atccgaccat gattggagcg
     3841 attagtggtc aagaagaagt aaatcaacgc ccttttactg ttgggcattt tgaccttatc
     3901 ataattgacg aatctcaccg ttctatttat cagaaataca agtccatttt tgattatttt
     3961 gatgcaagaa ttgtaggctt aacagctact ccgcgtcaag atttagataa aaacacctat
     4021 ggattcttta atttggagaa tggggttcca acatatgcat atgatttgga agaggctgtt
     4081 aaagacggat atttagtagc ctatcattct atcgaaacca aactgaaact acctacggat
     4141 ggtctacatt atgatgattt gtccgaagaa gaaaaggaac attttgatag caaatttgaa
     4201 gacgatagct gtgaaaaaga tattgatggg agtgtattta attcctttat tttcaataaa
     4261 agtacagtag aaattgtttt aaatgaactc atgacaagag gaattcagac agcctcgggt
     4321 gatgaaattg gtaaaactat tatttttgct aaaaatcatg atcatgcgga atatatcaga
     4381 ggtattttta acaaccgcta tcctgaaaaa gggagcgact atgctcaggt gattgattat
     4441 agtattaagc attatcagac cttgattgat gattttaaaa ttaaggagaa gtatcctcaa
     4501 attgcgattt ctgtcgatat gttagataca ggtattgatg taccagaggt tgttaattta
     4561 gtcttcttca agaaagtacg ctctaaaact aagttttggc agatgattgg tcgaggaacc
     4621 cgtctatgta aagatttatt tggacctgag caggataagg aaaacttctt ggtatttgat
     4681 tatggggaca attttgatta ttttcgtgca gatccaagag atggagaggg tcgtcacatt
     4741 gtttcgctga ctcagcgttt atttaatatc aaagtggact tgattcgaga acttcaggga
     4801 ctccaatacc aagaagatca gtttgcgaga gcataccgtc agcagcttgt ctcggaactt
     4861 caaggtcgta tagagagctt aaatgagttg gacttcaggg ttcgtatggt tttagataca
     4921 gtttatagct ataggaaatt ggaaagttgg cagaatctaa ctgctgttac aagtgaaacc
     4981 attcaaaaaa atctctctct gcttttattt gatgaagata aagaagatga gatggcgagg
     5041 agatttgatt tgtggttgct tcatattcag ttggggcaac tgacagctaa atcttccact
     5101 gttcatattt cccaagtgat gaagacggct agagctcttt ctgctattgg caatatcccg
     5161 caggtttttg agcaggctga aattatcagg aaagtacagg agcctgaatt ttggaaagaa
     5221 gttaacttgt ctgatttgga aaaaattcgt cttgctattc gagatttatt acagtttttg
     5281 gataaaacag accgtaaacc ctactatgtt aactttgaag atcgtatact ctccactgtt
     5341 cacgagacca cagcattttt gcaggtcaac gatcttcggt cttacaatga aaaagttgag
     5401 cattatttga aaactcatct ggatgaggag tccatttcta agctatacca taataaaaag
     5461 ttgacatctg atgatatgct tgcacttgaa aaattgcttt gggaaaaatt aggtagtaaa
     5521 gcagactacc aaagtcatta tgaaaataag gcaattccga gattggttcg tgagattatt
     5581 ggcttagata gagagtctgc caatcgtatt ttttctaaat ttttgtcgga tgagaatctt
     5641 aatgccaggc agatttcatt tgtaaaattg attgtagact acattgtaga aaatggtttt
     5701 ttagagacga aagtgttaac gcaagagccg tttaaatctt atggttctgt tcaactactc
     5761 ttccaacacc aactaccagt acttcgtaat attgttcaaa tcattgaact tatcaataat
     5821 cgagctggag aagcggctta aattctaaag tgattgccat gctgagactc atttaaaatt
     5881 aaaaagagta gaaatttatg ctatatatga gaagttttat taggaagaat gtcatcgttt
     5941 tcctagaata cagtatcagt tgttaagtgg ttgataaatt tcaaagtaga tacttgtacc
     6001 acgatgtttg ttgatcgagt tattaacaaa agagctactt tgattttaaa gaaatagaaa
     6061 acaaaaagcc gagcaagaat tcaattgcag gagaaaatga aataatactc aatgaaaatc
     6121 aaagagcaaa ctaggaaact agctgcaggc tgctcaaaac actgttttga ggttgtagat
     6181 ggaagctgac gcggattgaa gagattttcg aagagtataa atcttcctag gataaagcaa
     6241 aacgcatagt atcaagggtt ttcaacactt gatactatgc gttttctgat gttaaagact
     6301 ttctaccagg ttttttaaaa gcataattgt tagttgtagt catttattat tcttcaaaga
     6361 aaaatggtgg ggcgaatttt ttcagttctt caaagcactt ttgagcagta tctgcatctt
     6421 cacagatgat aagacagaca tcattaccac aaagggtagc gatagcgtca gggaagctca
     6481 aagtatcaat gatagaacca aaggattgag ccagtccagg aagggttttt agtaggactt
     6541 ggtgttgaac tgggcgcatc cagacaaggg cgtcttccat gtagagttcg agacgttttt
     6601 cccattttga gatggaacca ttgttaagaa cataataagc gctatcttct tcgcggactt
     6661 ttgataggtt catatttttg atgtcgcgtg agagggttgc ctgggttact tgaatgtcgt
     6721 tctcagcaag aagggcttgc aactcagcct gtgtatgaat cttgtttttt gtgataagag
     6781 cgcgtataag ttggtggcgg tgttctgatt tattcataat aatgtaactc cttttagcaa
     6841 ggtaaggtaa gcatggactg agcgaggtcg acagtcaagt cgtagtctgt attgtcacgg
     6901 atggtgattt caaagtcagt agtatagagg actaaacgga gagtgtctcc ttcttttagc
     6961 ttgtaaatag ttggctgcag ttcaaattga acgtccatcc attcatctgc agtaatatcc
     7021 tctactaaca gtaaatcatt tctattttgt aaattaaggt aaccttttgt cacgactcgt
     7081 tgtgcctctg gtctaaatgg caattcacag agattttcca acatgtgata gcgaccgttg
     7141 tcaatggttc tagcacttaa aatagctgga taaggttgta ggtatttctt ttgcccaaat
     7201 tctagcagtt gggcagataa gagccccttg tttgtactgg atttgatacg aagattgagc
     7261 tgagcgcgac cgtttaggtg gagatcttta gtcacaggaa ggttaatagt aatctgattg
     7321 gctttccctt gatagagctc tgtattgaag gtttggtatg tcttaccata gcgctcaaaa
     7381 tccttatctg ggtactggtt ttgaatagct tgctcttctt gaccaagtga gaaggtttca
     7441 cagttttctt gcccaccgaa gttatcaagt gataaccaag tctgtggagc tgtattgtcc
     7501 tgccagataa cagtaggaag ttgaaagtct gtttcctgtc ctagtaattt cttggtcaat
     7561 aaggcattta tggactcacg gaagtcaatt gattgccaat tgttcatgta aacatgagca
     7621 ccattatgga aaaagagatg cttgtgtata tgagtaggaa gagcatggaa catctggtaa
     7681 acatgaagtg gtttgacatt ccaatcctga gaaccatgag taaagacaac ctctgccttt
     7741 actttatggg cattgagcag ataattgcgg tcatgccaaa actgattgta gtccccagtt
     7801 tttcggtcta gctgagcttt cactttttct aagtcagctt ggtgagcttc attgccacgg
     7861 atatagtcgc cagctaagag attacgagaa taggttaact cagcaaggga gtcaaagtcc
     7921 tcacctggat aaccacctgg gctagtcacc agaccgtttt cacggtagta gttgtaccat
     7981 gatgaaattc ctgcctcggc aatgataact tctaaaccat cgactcctgt agtcgcaaga
     8041 ccattggaca tggtacctag ataggaaagt cccgttgtag caacttttcc gtttgaccaa
     8101 tcagccttga cttgacgctg gcgcgtgtga tcagtaaagg cacggcaacg gtcgttaagc
     8161 caatcgatga catttttata agcctcgatt tgctggtagt ctccattagt catgaaacct
     8221 gtcgagtctt tggtaccaac acctgagaca tagagagtgg caaagcctct cggaaggaag
     8281 tagtcgttta gtgtatagct agagttgatg tgagttagct tttcctcagc ctctgctaca
     8341 agctcagctt taccttgggg ttggacgaga tttagttgag gtttctctag ctcaatcttg
     8401 tgaggaagag aaacctcaag ctcgccctcc atcttgtaga gagccttgtc actagccttg
     8461 tcattggttc cctgatgata agggctggct gtcatgatgg cagggatttt tccatcaaaa
     8521 cgagggcgaa taatgctaac ctttactagg tctggtagcc ctttttggtc agtatcgaca
     8581 cgagactcaa cgtaaacgac ttcacgaatg acatcctggc tagaaaaagt agccaaactc
     8641 ttgccgttaa agtagtggta gtcattatcc tccggaataa gaccatcact aacaagttgg
     8701 tcgataagag tatttccttt tttggtgcga gtattgagta actgatagag attttcaatc
     8761 aagtcaccat atataatggg aaatccagtt tctttacgaa aaacgtcact atcttcgaag
     8821 tcaaccaaat aagaaaagcc taaaagttga aaagcaacag tataaaaaat atctgctgtc
     8881 agttcatctt ctgattgaaa aaatgtcagc aggtctgttt ttttatcagc tgctaggatg
     8941 gaaagtgggt agttggtgtc ttgataagtg aaaaagaaac gacgtaaaaa ggtttcaagt
     9001 gagtctttgt gattggctgt attttgtaaa tcaaagccac atttttttag ttcagataag
     9061 acattttctt ttggaaaatt gatataacta tattgattaa aacgcataga acctccatat
     9121 agaatgacag ttaaggttat tatatcaaaa aaaaagcaga aagggaattg ttaacttcaa
     9181 aaggaaataa tccaataaaa atgaataaag tactaaattc aatatagaga acagag
//
LOCUS       DAVMAF010000072         9054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMAF010000072 DAVMAF010000000
VERSION     DAVMAF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.073"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..201
                     /locus_tag="VYO97_001708"
     CDS             <1..201
                     /locus_tag="VYO97_001708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754470.1"
                     /translation="AQTRKLATGCSKHCFEVVDKTDEVSSKYCFEVADKTDEVSSKYC
                     FEVADKTDEVSSKYCFEVADGS"
     gene            complement(385..1233)
                     /gene="dprA"
                     /locus_tag="VYO97_001709"
     CDS             complement(385..1233)
                     /gene="dprA"
                     /locus_tag="VYO97_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1754471.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1329..2018)
                     /locus_tag="VYO97_001710"
     CDS             complement(1329..2018)
                     /locus_tag="VYO97_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1754472.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2030..2908)
                     /locus_tag="VYO97_001711"
     CDS             complement(2030..2908)
                     /locus_tag="VYO97_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1754473.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVVNGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2898..3767)
                     /gene="licA"
                     /locus_tag="VYO97_001712"
     CDS             complement(2898..3767)
                     /gene="licA"
                     /locus_tag="VYO97_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1754474.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3784..4806)
                     /locus_tag="VYO97_001713"
     CDS             complement(3784..4806)
                     /locus_tag="VYO97_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1754475.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPSGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMKVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4811..5518)
                     /locus_tag="VYO97_001714"
     CDS             complement(4811..5518)
                     /locus_tag="VYO97_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1754476.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVLHAEDLVDKYLPLHKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIKLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5873..7360
                     /gene="tacF"
                     /locus_tag="VYO97_001715"
     CDS             5873..7360
                     /gene="tacF"
                     /locus_tag="VYO97_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1754477.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7370..8173
                     /locus_tag="VYO97_001716"
     CDS             7370..8173
                     /locus_tag="VYO97_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1754478.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8175..8984
                     /locus_tag="VYO97_001717"
     CDS             8175..8984
                     /locus_tag="VYO97_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1754479.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKYDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLIIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2831 a         1819 c         1548 g         2856 t
ORIGIN      
        1 gcgcaaacta ggaagctagc cacaggttgc tcaaagcact gctttgaggt tgtagataag
       61 actgacgaag tcagctcaaa atactgtttt gaggttgcag ataagactga cgaagtcagc
      121 tcaaaatact gttttgaggt tgcagataag actgacgaag tcagctcaaa atactgtttt
      181 gaggttgcag atggaagctg acgtagtttg aagagatttt cgaagagtat tatatgagaa
      241 aaatcctcta ctctactaat ccacttagaa aaaacgcata ttatcaagtg ttaaaaactt
      301 gctaatatgc gttttattat ggaaatgttg ggttcatttt ctcttaaaat tgattttttc
      361 ttagaattct agcttaggtc atttttaaaa ttcaaattcc gcaagaacat cttgcccact
      421 ggtgaccaat tttgctcctt cttgaatcaa atgatggcaa ccgtctgata gtccatctaa
      481 aatgctacca ggaatagcaa agacatcgcg tccttcttcc attgctcgct cacacgtaat
      541 gagactacct gaacgcatct tagcctctgc tacaatcaca ccacgacaaa gtccagcaat
      601 gatgcgatta cgggcaggaa aatgaaattt cagaggttgt tcgccaggtc catattcact
      661 taaaaccaga tggtcattgc cgatgtagtc ttgcaagcgt ttattggctt taggataaaa
      721 cacatccagt cctgttccaa tcactgcaat ggtttttccg ccattctgaa gagctgccat
      781 atgagctgct gtgtcaatgc ccttggccag accactgaca ataaccagtt cattttccaa
      841 gccttgaatg actttttcaa ctgatttagc tccctgtttg ctacaagcac gactgcccac
      901 gaccgctacc ttcgggaatt tcaggaggtc aagatttccc ttgtaaaata aaagtacagg
      961 cgcatcatat atttcactca aatcccaagg ataacagtca tctaaaatag agaaagatgg
     1021 aaatttttga aactctttcg acaaatgcgc atcgtctatc tgaaaataac gttccataaa
     1081 aacggctgga ttacgacaac ctgatatatc tgcaatatca cccaacaaaa gctcctgatc
     1141 aacattttca ccgtattcta gcactttcaa aatctgttga ttggtcaaac ctgatttttt
     1201 taacttatag atttcatagt ttgtgatttt cataaataac tccatttctt tttctactca
     1261 tctatctatt cgtaaaagtt cgtaaaaaat caaaaaacat ccgactattt tgtcagatgt
     1321 tggaatcttt aattttcgtt tttaagaatt tcttctaatt tacgatagtc ttggacacta
     1381 tcgatctcat aaatgctatt gccttctaat tcttcaacat agacatctag ctctttgata
     1441 ttatccttaa ccatattgtc ccagtagaga tcaacaaatt cgccacttgc ataagccttg
     1501 tcgataaagc tgacaatctt ttctgcagtt ggagcatccc agaaggatac accactaagg
     1561 atgcgacctg ccttgctatc aacaataatg tcttgaacct tgtagtcatc tccatagacc
     1621 aagaaccatt cgttggtaca atcttcacga taaacactaa aataagtcga acgagtcaaa
     1681 tcattgcgga acatattttt aaagagatag ttatctgcat caataacata gctgttggcc
     1741 aattcttctt ttacaagata gagagagtaa aagttattgt agtcagcgta tttatcattg
     1801 aaaacgaggc gaacaccgta tttttctttt aagtaatcga attgttcttt aagataacca
     1861 acaatgatga tgatgtcatt gattcctttt tctttgagaa actcaatttg gtattcaatc
     1921 aaaggttttt gattaacctg aaccaaggct ttaggggtat tttcagtcat aggacgcaag
     1981 cgagttccca atcccgctgc taagataatg gctttcacac gaatctcctt tattctttaa
     2041 taataatata aactccagca atgacgacaa gtgacgtcat aatggtcagc atatctagcg
     2101 gtgcacccaa aaaaacaact gcaaacaaga ccgtccatac tacatagctc acgttcaagc
     2161 ctgtagcctt ggctggttgc aagcgattga tagcgatata ataagccaag taggaaatca
     2221 tatcaaaggc tgcaaaaaca atcatgagac ctagcaattg tccattgact actgcagtaa
     2281 atgactgatg agagaagagc acaatcacaa gataggacaa gaacgaagtc acttgacgga
     2341 ttaagagggc ttcgatttca ctcagttcac tttccatagc aaaggagcta agaacactct
     2401 cacttcccca tgcaatagca caaaccaaag cacagagaat cccaatgtag aaggaattaa
     2461 cctgttcaac cttataggtc tgggcaataa tccctccaat aatcaagaca atcccaaata
     2521 cagtattttt cgaaatcttg tgcttcaaaa agaagaaagc caatagaact gaaatcgcag
     2581 ggtaaatagc cgatacagat gaagctaaag aacttccgat atacttaact gcataaagat
     2641 tggcctgcat accgataggg cctgctagca aggctccgat gataacactg acattgcgaa
     2701 tatttaagaa aattgagagg cgaactttcc cttcttttac caagagaaaa gctagtaaga
     2761 taaagatgct caaaaaatca tgagctgcag ccaccacaaa gggtgacaaa tctgtaaaaa
     2821 tcgaaaagat ataagcacta accgttagac ccaagcccca gaaaatacct gagagaaggc
     2881 caaaaggaac tccgttttta cttttcatct gatcctccat aagaagccaa acctttaaca
     2941 gctctttggt aacgattcac accatagtaa ccaaaatctg caccttgctc ttccttatag
     3001 acagtccata gactccaaat agtatcttgt aaaattttat aaatagcaat cttttcatga
     3061 gaaaccggtg tttggtcact ctcatagtga gataagaaag tttcttcctc ttgggaagtg
     3121 aattcagact ctaaaaagag ggcagccaaa tcccacattg gatcattcat tgatgaatat
     3181 tcccagtcaa tcaaataaag tcgtccttga ggtgattcga taaagttttc aggcaccaaa
     3241 tcgatatgac aagattttct gtcaacacct aagtcagcca gtcttttctc taaggagaag
     3301 actgcatttc taacagattc atagttggca taaggaattt gttcttcaat caaggattcg
     3361 tattttttga tttcttcaaa aggagcaaat tctcctctta attccttagc agatgtatga
     3421 atagtttgta atattggagc aattttgtcg aacttggtct tgattgacgt tgaatcaagc
     3481 gtaatcgcag attcgatata ctcatttact ttgatgccag cttcaatatc aaaaagataa
     3541 ttttttacat ctaagcctaa atcctttagt agttcaagat tgtacttttc atcttgtcga
     3601 ttgataagct tttctgtccc tttaccaaag aatttaacaa tgtattgctt atttgttgtt
     3661 ttggccaaat agttttgatt ggtcattcca cccagttgtt caacactgag gacttcttct
     3721 tcttgactaa gtaaggaaga aattttttct ttaatgattt tctccacaat taacctccag
     3781 gacttatact tcccacttaa acactgtttt aaaggctgtg tttaaatcgg ttgcaaagac
     3841 acggtgaata tctttaattt ctcttacagg ttcttctaga taaaggatat ttttaagacg
     3901 attggcaaat ttcttgactt tcatcatttg gatagcattt tcaaaatcaa tgcgaccaga
     3961 acgagatgac ccaaccaaga gcaagccctt ttctaaggca tcgcgagtat tgagattgac
     4021 tttatattcg ctaactccca tcatgagaat tgttccctga ggacgaatgt agcgaatcaa
     4081 gtcattaata gctggtccag taccatcacc accacaacat tcaaaagcat ggtcaaaggc
     4141 caactcttca ggaatattat ccgtaatata gcattctttg gcaaatgaga agagttccaa
     4201 cttttcccaa tgacgaccaa taaccacaat ctctgcttct ggcaaagtat agttgataat
     4261 attggcaacc acaaaagcta aacttccatc tccaataacg gcgatccgct cccgcttgct
     4321 atgagcaaga gtcaatagac gattcatagc gtgcatgccc acactgacaa actctgtaat
     4381 ggctgcaacc gtatcttcaa tagcatcata agccaccaca cgatctttag ggagagaaac
     4441 aaactctctc ataaagccat caaatccact agacaagaaa tgggtccctg tcatgtagtt
     4501 ttcatagaat tcttcatcac tctgcatagg agactgattg ggaatcatga caactttttg
     4561 accaacctcg taggttccgc tcgggtcaga aatgacggtt ccacatgact cgtgaatcat
     4621 tgccattgga agctttttat tcaaaatctt gggatcacgt tttccctggt agtaacgctg
     4681 atccgcatga cagacagcca tgtagttggg acggataagg atatgattct cttggtcaat
     4741 agcctcttcc tgatatttga cattgataaa cttaggttta gttagttgat aaatttgatt
     4801 aatcatttta ctagtctttc tcaatcatac tttttgcaat cttcaaatct gttacggttg
     4861 taatcttcag atttgagtat tcgcctttgg ccaaggctac atcttttcct ttgatcacaa
     4921 agattttaca tgcatctgtc aagatttcct tctcttcatc agaaagagat ccataaaggt
     4981 ccatgaagtc cttgcaacgg aatgtttgag gtgtttgtcc ctgatagagg tgagcacgat
     5041 ttggaatacc tgtaatgaat tgaccattgg tactttcaac gatagtatcc actgcttcta
     5101 ctactgtatc cactgcgtca tgattttgag caagtttgat actgtcttga atcatgcgaa
     5161 gcgtaataaa tggacgaaca gaatcgtggg taacaacgat atcctctgga gtaagcgggc
     5221 gataagcatc aatggcttca atgatgttct caatacttgt attgcggtca gcaccaccct
     5281 ttgtaatgat aatacgttcc ttatgaagag gaagatattt atctacaaga tcttctgcat
     5341 gtaaaaccca gtctccatga accccaacta caattttttc aatacttggt tccaagacaa
     5401 atttttcaat tgtatgaatc aaaataggtc gatcacctag ctctaaaaat tgttttggca
     5461 agttactgat tcccatgcgt gtgccagttc caccggcaag aattcctgca taaatcatct
     5521 attttctcct ttgtcttact aaaaaactta ttctctctat tataccacaa tctagtccta
     5581 tataccacaa tctagtccta tcatgaaaga aatactggtc ctccccttct agaataggag
     5641 aattatgcag ttctattatt caaagaaaca agcctatttc tagtaatgac taggaattct
     5701 gtagcaatta ctaataaatg actgcgaaat tttagagtcc ttcagaataa tatactttcc
     5761 ttaaagaaaa cttaatattt aagatttgaa acttcaaagc gattgtttga cttgctgccc
     5821 ttcatttctt attagctaac tttatgatat aatgaaaaac gaggtaaatt gaatgaaaag
     5881 tataaaatta aatgctctat cttacatggg aattcgtgtc ttgaatatta tttttcccat
     5941 cctaactgga acctatgtcg cgcgtgtctt ggaccgaact gactatggtt acttcaactc
     6001 agtcgacact attttgtcat ttttcttgcc ctttgcaact tatggtgtct atagctacgg
     6061 tttaagggct atcagtaatg tcaaggataa caaaaaagat cttaacagaa ccttttctag
     6121 tcttttttat ttgtgcatcg cttgtacgat tttgaccact gctgtctata tcctagccta
     6181 tcctctcttc tttactgata atccaatcgt caaaaaggtc taccttgtta tggggattca
     6241 actcattgcc cagatttttt caatcgaatg ggtcaatgaa gctctggaaa attacagttt
     6301 tctcttttac aaaactgcct tcatccgtat cctgatgctg gtctctattt tcttatttgt
     6361 taaaaatgaa cacgatattg ttgtctatac acttgtgatg agtttatcga cgctgattaa
     6421 ctacctgatt agttattttt ggattaaaag agacatcaaa cttgttaaaa ttcacctaag
     6481 tgattttaaa ccactctttc tccctctgac agccatgtta gtctttgcca atgccaatat
     6541 gctcttcact tttttagatc gcctcttcct cgttaaaaca gggattgatg tcaacgttag
     6601 ttactatacc atagctcagc gaattgtgac cgttatagct ggggttgtaa caggtgcaat
     6661 tggagtgagt gtgcctcgtc tcagttacta tctggggaaa ggagacaaag aagcctatgt
     6721 ttctctggtt aatagaggta gtcgaatctt taacttcttt atcattccac tgagttttgg
     6781 actcatggtt ttaggaccaa atgccatcct actttacggt agtgaaaaat atatcggagg
     6841 cggcatcttg acctctctct tcgcttttcg tacgattatc ctggccttag ataccattct
     6901 tggttcccaa attctcttta ccaatggcta tgaaaaacgt atcacagtct atacagtctt
     6961 tgctgggcta ctcaatttgg gcttgaatag tctccttttt ttcaaccata tcgtggctcc
     7021 tgaatactac ttactgacaa ctatgctatc agagacttct ctacttgttt tctatatcat
     7081 tttcatccat agaaaacaac tcatccactt gggacatatc tttagctata ctgttcgata
     7141 ctctctcttt tcactttcct ttgtagcaat ttatttcctg attaatttcg tgtatcctgt
     7201 agatatggtc attaatttgc catttttgat taatactggt ttgattgtct tgctatcagc
     7261 tatctcttat attagtctac ttgtcttcac aaaagatagc attttctatg aatttttaaa
     7321 ccatgtccta gccttaaaaa ataaatttaa aaaatcatag gagtttaaaa tgaaacaact
     7381 aaccgttgaa gatgccaaac aaattgaatt agaaattttg gattatattg atactctctg
     7441 taaaaagcac aatatcaact atattattaa ctacggtact ctgattgggg cggttcgaca
     7501 tgagggcttt atcccttggg acgacgatat tgatttatcc atgcctagag aagactacca
     7561 acgatttatt aacatttttc aaaaggaaaa aagcaagtat aagctcctat ccttagaaac
     7621 tgataagaac tactttaaca actttatcaa gataaccgac agtacgacta aaattattga
     7681 tactcgaaat acaaaaacct atgagtctgg tatctttatc gatattttcc ctatagatcg
     7741 ctttgatgat cctaaggtca ttgatacttg ttataaactg gaaagcttca aactgctgtc
     7801 tttcagtaaa cataaaaata ttgtctataa ggatagcctt ttaaaagatt ggatacgaac
     7861 agccttttgg ttgctccttc gaccggtttc tcctcgttat tttgcaaata aaatcgagaa
     7921 agaaattcaa aaatatagtc gtgaaaatgg gcagtatatg gcttttattc cttcaaaatt
     7981 taaggaaaag gaagtcttcc caagtggtac ctttgataaa acaatcgatt taccctttga
     8041 gaatttaagc cttcctgcac ctgaaaaatt tgatactatt ttgacacaat tttatggaga
     8101 ttatatgacc ctaccaccag aagaaaaacg cttctacagt catgaatttc acgcttataa
     8161 attggaggat taggatgcaa tatttagaaa aaaaagaaat taaagaaatt caactagccc
     8221 tgctggacta tattgatgag acttgtaaga aatatgatat tccttatttt ctcagttatg
     8281 gaaccatgct tggagccatc cgccacaaag gtatgattcc ttgggatgat gatattgata
     8341 tttcccttta tcgtgaggat tatgagcgtt tactgaagat tattgaagaa gaaaatcacc
     8401 ctcgctacaa ggttctttcc tacgatacat cttcttggta cttccataat ttcgcatcga
     8461 ttttggacac ttctactgtt atagaagacc atgttaagta caagcgtcat gataccagcc
     8521 ttttcatcga tgtcttccca attgatcgat ttacagactt gatcattgtc gacaagagct
     8581 ataagtatgt ggctcttcgt caactagctt atatcaaaaa atcacgagca gttcacggtg
     8641 atagcaaact aaaagatttt cttagattat gtagctggta cgctctccga tttgtcaatc
     8701 ctcgctactt ttacaagaaa attgatcaac tagtcaaaaa tgctgtaacc aacactcctc
     8761 aatatgaagg aggagttggg atcggtaagg aagggatgaa agaaatcttc ccagttgata
     8821 cctttaaaga actgatttta actgagtttg agggccgtat gttgcctgtt cccaaaaaat
     8881 atgaccaatt tttaacccag atgtatggcg attatatgac accaccatca aaagaaatgc
     8941 aagagtggta tagtcatagc attaaagctt atcgcaaaaa ctgattgagg gggattatac
     9001 aaactactaa gatagaggtt attcaaaaac ataattttag tagaaaatga aata
//
LOCUS       DAVMAF010000073         8995 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMAF010000073 DAVMAF010000000
VERSION     DAVMAF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.074"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            206..2857
                     /locus_tag="VYO97_001718"
     CDS             206..2857
                     /locus_tag="VYO97_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV1754480.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTTVETKEASTPL
                     DVVILLDNSNSMSNIRHNHAHRAEKAGEATRALVDKITSNPDNRVALVTYGSTIFDGS
                     EATVEKGVADANGKILNDSALWTFDRTTFTAKTYNYSFLNLTSDPTDIQTIKDRIPSD
                     AEELNKDKLMYQFGATFTQKALMTADDILTKQARPNSKKVIFHITDGVPTMSYPINFK
                     YTGTTQSYRTQLNNFKAKTPNSSGILLEDFVTWSADGEHKIVRGDGESYQMFTKKPVT
                     DQYGVHQILSITSMEQRAKLVSAGYRFYGTDLYLYWRDSILAYPFNSSTDWITNHGDP
                     TTWYYNGNMAQDGYDVFTVGVGVNGDPGTDEATATRFMQSISSSPDNYTNVADPSQIL
                     QELNRYFYTIVNEKKSIENGTITDPMGELIDFQLGADGRFDPADYTLTANDGSSLVNN
                     VPTGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSNKFY
                     DTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPEITIPKEKKLGEIEFIKINKNDKKP
                     LRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENSEPA
                     GYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREYPRT
                     GGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3090..5024
                     /locus_tag="VYO97_001719"
     CDS             3090..5024
                     /locus_tag="VYO97_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEV1754481.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAADNVSTAPDAVTKTL
                     TIHKLLLSEDDLKTWDTNGPKGYDGTQSSLKDLTGVVAEEIPNVYFELQKYNLTDGKE
                     KENLKDDSKWTTVHGGLTTKDGLKIETSTLKGVYRIREDRTKTTYVGPNGQVLTGSKA
                     VPALVTLPLVNNNGTVIDAHVFPKNSYNKPVVDKRIADTLNYNDQNGLSIGTKIPYVV
                     NTTIPSNATFATSFWSDEMTEGLTYNEDVTITLNNVAMDQADYEVTKGINGFNLKLTE
                     AGLAKINGKDADQKIQITYSATLNSLAVADIPESNDITYHYGNHQDHGNTPKPTKPNN
                     GQITVTKTWDSQPAPEGVKATVQLVNAKTGEKVGAPVELSENNWTYTWSGLDNSIEYK
                     VEEEYNGYSAEYTVESKGKLGVKNWKDNNPAPINPEEPRVKTYGKKFVKVDQKDTRLE
                     NAQFVVKKADSNKYIAFKSTAQQAADEKAAATAKQKLDAAVAAYTNAADKQAAQALVD
                     QAQQEYNVAYKEAKFGYVEVAGKDEAMVLTSNTDGQFQISGLAAGTYKLEEIKAPEGF
                     AKIDDVEFVVGAGSWNQGEFNYLKDVQKNDATKVVNKKITIPQTGGIGTIIFAVAGAA
                     IMGIAVYAYVKNNKDEDQLA"
     gene            5071..6252
                     /gene="rrgC"
                     /locus_tag="VYO97_001720"
     CDS             5071..6252
                     /gene="rrgC"
                     /locus_tag="VYO97_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEV1754482.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDDRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKTDTMTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIFVTNLPLGNY
                     RFKEVEPLAGYAVTTLDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEESGHYTPVLQNGKEVVVTSGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            6590..7492
                     /gene="srtC-1"
                     /locus_tag="VYO97_001721"
     CDS             6590..7492
                     /gene="srtC-1"
                     /locus_tag="VYO97_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEV1754483.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7479..8372
                     /gene="srtC-2"
                     /locus_tag="VYO97_001722"
     CDS             7479..8372
                     /gene="srtC-2"
                     /locus_tag="VYO97_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEV1754484.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGKNTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8359..>8995
                     /gene="srtC-3"
                     /locus_tag="VYO97_001723"
     CDS             8359..>8995
                     /gene="srtC-3"
                     /locus_tag="VYO97_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEV1754485.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTF"
BASE COUNT         2921 a         1515 c         2082 g         2477 t
ORIGIN      
        1 aagtgtgtca ttgccaggtt gagaagatag ctataacgca cttttatacg cttttgctac
       61 gtttgttagt gaacggatta actcagtgag ataaatttta tcagaacata agtaatccgt
      121 ttcttcgtgt atacagattg aaagtaccta tgaatcatag aaggattaac ttgttctatg
      181 aataatgctt aacagggaga cacacatgaa aaaagtaaga aagatatttc agaaggcagt
      241 tgcaggactg tgctgtatat ctcagttgac agctttttct tcgatagttg ctttagcaga
      301 aacgcctgaa accagtccag cgataggaaa agtagtgatt aaggagacag gcgaaggagg
      361 agcgcttcta ggagatgccg tctttgagtt gaaaaacaat acggatggca caactgtttc
      421 gcaaaggaca gaggcgcaaa caggagaagc gatattttca aacataaaac ctgggacata
      481 caccttgaca gaagcccaac ctccagttgg ttataaaccc tctactaaac aatggactgt
      541 tgaagttgag aagaatggtc ggacgactgt ccaaggtgaa caggtagaaa atcgagaaga
      601 ggctctatct gaccagtatc cacaaacagg gacttatcca gatgttcaaa caccttatca
      661 gattattaag gtagatggtt cggaaaaaaa cggacagcac aaggcgttga atccgaatcc
      721 atatgaacgt gtgattccag aaggtacact ttcaaagaga atttatcaag tgaataattt
      781 ggatgataac caatatggaa tcgagttgac ggttagtggt aaaacgacgg ttgaaacgaa
      841 agaagcctct actccgctag atgttgttat tctattagat aactccaata gtatgagtaa
      901 tattcgacat aatcatgccc atcgagcgga aaaagcggga gaagcgacac gagcccttgt
      961 agataagatt acctccaatc cagataatcg agtagcactt gtgacttatg gctcaactat
     1021 ctttgacggt tcagaagcta ctgtggaaaa aggggtagca gatgcgaacg gaaaaatatt
     1081 gaatgactca gctttatgga cgttcgatcg tacgacgttt acagctaaaa cttataatta
     1141 tagcttttta aatctcacat cagatcctac tgatattcaa actattaagg ataggattcc
     1201 atcagatgca gaggaattga acaaagacaa attgatgtat caattcggcg cgacttttac
     1261 ccagaaggct ttgatgaccg ctgatgatat cttgacaaag caggcaagac caaacagtaa
     1321 aaaggttatt ttccacatta cagatggtgt tccgactatg tcatatccaa ttaattttaa
     1381 atatacagga acgacgcaat cgtacagaac tcagctgaat aattttaaag caaaaactcc
     1441 aaatagtagc gggatattac tggaggactt tgttacatgg tcagcagatg gtgaacataa
     1501 gattgttcgt ggagatggtg aaagttatca gatgtttacg aagaaacctg taacagacca
     1561 atacggagtt catcaaatac tttcaatcac ctccatggag cagagagcta aattagtttc
     1621 agcgggatat aggttctatg gaactgactt gtatttatat tggcgtgata gtattctagc
     1681 ctatccattt aactctagta ccgattggat taccaaccat ggtgacccta cgacttggta
     1741 ttataacgga aatatggctc aggatggcta tgatgtcttc actgttgggg ttggtgtaaa
     1801 cggggatcct ggtacggatg aagcaacggc tactagattt atgcagagca tctctagttc
     1861 tcctgacaac tacactaacg tagcagatcc atctcagatt ttacaagaat tgaatcgcta
     1921 cttctatact atcgtcaatg agaagaaatc tatcgaaaat ggtacgatta cagacccgat
     1981 gggtgaacta attgatttcc aattgggagc agatggaagg tttgatccag cggattacac
     2041 tttaactgca aacgatggta gttcgttggt gaataatgtc cctactgggg gaccacaaaa
     2101 tgatggtggc ttgctaaaaa atgcaaaagt gttctatgat acgactgaga aaaggattcg
     2161 tgtaacaggt ttgtaccttg gaacgggtga aaaagttaca ttgacttata atgttcgctt
     2221 gaatgaccaa tttgtaagca ataaattcta tgacacgaat ggtcgaacaa ccctacaccc
     2281 taaggaagta gaaaagaaca cagtgcgcga cttcccgatt cctaagattc gtgatgtacg
     2341 aaagtatcca gaaatcacaa ttccaaaaga gaaaaaactt ggtgaaattg agtttattaa
     2401 gatcaataag aatgataaaa aaccactgag agatgcggtc tttagtcttc aaaaacaaca
     2461 tccggattat ccagatattt atggagctat tgatcaaaat ggcacttatc aaaatgtgag
     2521 aacaggtgaa gatggtaagt tgacctttaa aaatctgtca gatgggaaat atcgattatt
     2581 tgaaaattct gaaccagctg gttataaacc cgttcaaaat aagcctatcg ttgccttcca
     2641 aatagtaaat ggagaagtca gagatgtgac ttcaatcgtt ccacaagata taccagcggg
     2701 ttacgagttt acgaatgata agcactatat cacaaatgag ccaattcctc caaaaagaga
     2761 atatcctcga actggtggta tcggaatgtt gccattctat ctgataggtt gcatgatgat
     2821 gggaggagtt ctattataca cacggaaaca tccgtaaagt gtagcaatga gaaatgataa
     2881 tatcgatact ctgagcgata cttttaagaa gtagcactca agaagagatt taagtttact
     2941 tggtgaaaac agttttcttc gccaagtaaa ccaccattga aaggggagat gttttcgaaa
     3001 acttgcacag aaaaaaggat tattattgtc atgtgtaatt cattacattg ctcacagttg
     3061 attttaagag atatgaataa ggagaaatca tgaaatcaat caacaaattt ttaacaatgc
     3121 ttgctgcctt attactgaca gcgagtagcc tgttttcagc tgcaacagtt tttgcggcgg
     3181 acaatgttag tacagcacca gatgctgtta ctaaaacttt aacaatccat aagttactgc
     3241 tctcagaaga tgatttaaag acttgggata caaacggtcc taaaggatat gatggaactc
     3301 aatctagttt aaaagattta actggagttg tagctgagga aattccaaat gtatactttg
     3361 aattacaaaa gtataatttg actgatggta aggaaaaaga aaatcttaaa gatgatagta
     3421 aatggacaac agttcatggt ggtttgacaa ctaaagatgg acttaaaatt gaaaccagta
     3481 ctcttaaagg tgtgtatcgt attcgtgagg atagaacaaa gactacctat gttggtccta
     3541 atgggcaagt attaacaggt tcaaaagccg tacctgctct tgtaactctt ccacttgtta
     3601 acaataatgg tacagtaatt gatgcacatg ttttccctaa aaattcatat aataaaccag
     3661 ttgtagataa aagaattgct gatactttga attataacga tcaaaatggt ctgtctatcg
     3721 gtactaaaat cccatatgtt gttaatacaa caattccaag taatgcaaca tttgcaactt
     3781 cattttggtc agatgaaatg acagaaggtc taacttataa tgaagatgta acaattactt
     3841 tgaataatgt agctatggat caagctgatt atgaagtcac taaaggaatt aatggcttta
     3901 acttaaaatt aacagaagca ggtttagcta aaattaatgg taaggatgca gaccaaaaaa
     3961 tccaaattac ttactcagct actttgaact cacttgctgt tgcagacatt cctgaaagta
     4021 acgatattac atatcattac ggaaatcatc aagatcatgg gaatactcca aaaccaacta
     4081 aacctaataa tggtcaaatt acagtaacta agacatggga cagtcaacct gctcctgagg
     4141 gggtgaaagc gactgttcaa cttgtaaatg ccaagactgg tgagaaagtc ggtgctcctg
     4201 tagaactttc agaaaataat tggacatata cttggagtgg tctagataat tctattgaat
     4261 acaaagttga agaagaatat aatggatact cagctgaata cacagtagag agcaaaggga
     4321 agttgggggt aaaaaactgg aaagataata acccagctcc aatcaatcct gaagaaccac
     4381 gtgtaaaaac atacggtaaa aagtttgtca aagtagacca aaaagatact cgtctagaaa
     4441 atgcgcagtt cgttgttaaa aaagcagata gcaataaata tattgccttt aagtcaactg
     4501 cacaacaagc tgcagatgaa aaagcagcag caactgcaaa acaaaaattg gatgcagcgg
     4561 tagcagctta cacaaatgct gcagataagc aagccgctca agctctagta gatcaagcac
     4621 agcaagaata caatgtagct tacaaagaag ccaaatttgg ttatgttgaa gtagctggaa
     4681 aagatgaagc aatggttctt acttctaata cggatggtca attccaaatt tcaggtcttg
     4741 ctgctggtac ttataaatta gaagaaatta aagctccaga aggttttgcg aaaattgatg
     4801 atgtagaatt tgttgttgga gcaggttctt ggaatcaagg tgagtttaat tacttaaaag
     4861 atgttcaaaa gaatgacgct acaaaagtag tcaacaaaaa aatcactatc ccacaaacgg
     4921 gtggtattgg tacaattatc tttgctgtag cgggggctgc gattatgggt attgcagtgt
     4981 acgcatatgt taaaaacaac aaagatgagg atcaacttgc ttaagtaaga gagaaaggag
     5041 ccattgatga caatgcagaa aatgcagaaa atgattagtc gtatcttctt tgttatggct
     5101 ctgtgttttt ctcttgtatg gggtgcacat gcagtccaag cgcaagaaga tcacacgttg
     5161 gtcttgcaat tggagaacta tcaggaggtg gttagtcaat tgccatctcg tgatggtcat
     5221 cggttgcaag tatggaagtt ggatgattcg tattcctatg atgatcgggt gcaaattgta
     5281 agagacttgc attcgtggga tgagaataaa ctttcttctt tcaaaaagac ttcgtttgag
     5341 atgaccttcc ttgagaatca gattgaagta tctcatattc caaatggtct ttactatgtt
     5401 cgctctatta tccagacgga tgcggtttct tatccagctg aatttctttt tgaaatgaca
     5461 gatcaaacgg tagagccttt ggtcattgta gcgaaaaaaa cagatacaat gacaacaaag
     5521 gtgaagctga taaaggtgga tcaagaccac aatcgcttgg agggtgtcgg ctttaaattg
     5581 gtatcagtag caagagatgg ttctgaaaaa gaggttccct tgattggaga ataccgttac
     5641 agttcttctg gtcaagtagg gagaactctc tatactgata aaaatggaga gatttttgtg
     5701 acaaatcttc ctcttgggaa ctatcgtttc aaggaggtgg agccactggc aggctatgct
     5761 gttacgacgc tggatacgga tgtccagctg gtagatcatc agctggtgac gattacggtt
     5821 gtcaatcaga aattaccacg tggcaatgtt gactttatga aggtggatgg tcggaccaat
     5881 acctctcttc aaggggcaat gttcaaagtc atgaaagaag aaagcggaca ctatactcct
     5941 gttcttcaaa atggtaagga agtagttgta acatcaggga aagatggtcg tttccgagtg
     6001 gaaggtctag agtatgggac atactattta tgggagctcc aagctccaac tggttatgtt
     6061 caattaacat cgcctgtttc ctttacaatc gggaaagata ctcgtaagga actggtaaca
     6121 gtggttaaaa ataacaagcg accacggatt gatgtgccag atacagggga agaaaccttg
     6181 tatatcttga tgcttgttgc cattttgttg tttggtagtg gttattatct tacgaaaaaa
     6241 ccaaataact gatattcaat gtacatcatt atgaaaaaga tagcaggctg aagggaagac
     6301 cagagtactc tgaggtgatg ttaatcagga atcatggtga tgtggcatga atcacaataa
     6361 cggatatgag gctgggcaga ttgtgccagc ctcattgtgg gttattgttt gtaaaacgat
     6421 aggactggtc tggtaatcat tttaggaatg gacaggactg ggattctgat ttaaaatgga
     6481 tggtgaatca gaaagaaatg agattttctc gtttctctta gcagatagga ttgtctgtta
     6541 ggaaaagcga taaaatgatg agtttgaaga taaagggatg ctgataaaaa tggtaaaaac
     6601 aaaaaagcaa aaacgaaata atctcctatt aggagtggta tttttcattg gaatggcggt
     6661 aatggcgtat ccgctggtgt ctcgcttgta ttatcgagtg gaatcaaatc aacaaattgc
     6721 tgactttgat aaggaaaaag caacgttgga tgaggctgac attgatgaac gaatgaaatt
     6781 ggcacaagcc ttcaatgact ctttgaataa tgtagtgagt ggcgatcctt ggtcggaaga
     6841 aatgaagaaa aaagggcgag cagagtatgc acgtatgtta gaaatccatg agcggatggg
     6901 gcatgtggaa atccccgtta ttgacgtgga tttgccggtt tatgctggta ctgctgaaga
     6961 ggtattgcag caaggggctg ggcatctaga gggaacttct ctgccgatcg gaggcaattc
     7021 gacccatgcg gtgattacgg cacatacagg tttgccaaca gctaagatgt ttacggattt
     7081 gaccaaactt aaagttgggg ataagtttta tgtgcacaat atcaaggaag tgatggccta
     7141 tcaagtggat caagtaaagg tgattgagcc gacgaacttt gatgatttat tgattgtacc
     7201 aggtcatgat tatgtgacct tgctgacttg tacgccatac atgatcaata cccatcgtct
     7261 attggttcgg gggcatcgga taccgtacgt agcagaggtt gaggaagaat ttattgcagc
     7321 aaacaaactc agtcatctct atcgctacct gttttatgtg gcagttggtt tgattgtgat
     7381 tcttttatgg attattcgac gcttgcgcaa gaagaaaaaa caaccggaaa aggctttgaa
     7441 ggcgctgaaa gcagcaagga aggaagtgaa ggtggaggat ggacaacagt agacgttcac
     7501 gaaaaaaagg cacaaaaaag aagaaacatc cgctgatcct tcttctgatt ttcttagtag
     7561 gattcgccgt tgcgatatat ccattggtgt ctcgttatta ttatcgtatt gagtcaaacg
     7621 aggttattaa agagtttgat gagacggttt cccagatgga taaggcagaa cttgaggagc
     7681 gttggcgctt ggctcaagcc ttcaatgcga ccttgaaacc atctgaaatt cttgatcctt
     7741 ttacagagca agagaaaaag aaaggcgtct cagaatatgc caatatgcta aaggtccatg
     7801 agcggattgg ctatgtggaa attcctgcga ttgatcagga aattccgatg tatgtcggaa
     7861 cgagtgagga cattcttcag aaaggggcag ggctgttaga aggggcttcg ctgcctgttg
     7921 gaggtaaaaa tacccataca gtgatcactg ctcacagagg attgccaacg gcagaattgt
     7981 tcagtcaatt ggataagatg aaaaaagggg atatctttta tcttcacgtt ttagatcagg
     8041 tgttggccta ccaagtggat cagatagtga cggtggagcc gaatgacttt gagcctgtct
     8101 tgattcaaca tggggaagat tatgcgacct tgttgacttg tacaccgtat atgattaaca
     8161 gtcatcgtct gttggtacgt gggaagcgga ttccgtatac ggcaccaatt gcagagcgga
     8221 atcgagcggt gagagagcgt gggcaattct ggttgtggtt attactagga gcgatggcgg
     8281 tcatccttct cttgctgtat cgcgtgtatc gtaatcgacg gattgtcaaa ggactagaaa
     8341 agcaattgga ggggcgtcat gtcaaggact aaactacgag ccttattggg atacttgttg
     8401 atgttggtag cctgtttgat tcctatttat tgttttggac agatggtgtt gcagtctctt
     8461 ggacaggtga aaggtcatgc tacatttgtg aaatccatga caactgaaat gtaccaagaa
     8521 caacagaacc attctctcgc ctacaatcaa cgcttggctt cgcaaaatcg cattgtagat
     8581 ccttttttgg cggagggata tgaggtcaat taccaagtgt ctgacgaccc tgatgcagtc
     8641 tatggttact tgtctattcc aagtttggaa atcatggagc cggtttattt gggagcagat
     8701 tatcatcatt tagggatggg cttggctcat gtggatggta caccgctgcc tctggatggt
     8761 acagggattc gctcagtgat tgctgggcac cgtgcagagc caagccatgt ctttttccgc
     8821 catttggatc agctaaaagt tggagatgct ctttattatg ataatggcca ggaaattgta
     8881 gaatatcaga tgatggacac agagattatt ttaccgtcgg aatgggaaaa attagaatcg
     8941 gttagctcta aaaatatcat gaccttgata acctgcgatc cgattcctac cttta
//
LOCUS       DAVMAF010000074         8818 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMAF010000074 DAVMAF010000000
VERSION     DAVMAF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.075"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            209..1258
                     /locus_tag="VYO97_001724"
     CDS             209..1258
                     /locus_tag="VYO97_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1754486.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLMANLNLKFSVRNKSRNRFK
                     FCRSFKYPARRCSWC"
     gene            1230..1874
                     /locus_tag="VYO97_001725"
     CDS             1230..1874
                     /locus_tag="VYO97_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1754487.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1916..3904)
                     /locus_tag="VYO97_001726"
     CDS             complement(1916..3904)
                     /locus_tag="VYO97_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754488.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFKKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3906..4664)
                     /locus_tag="VYO97_001727"
     CDS             complement(3906..4664)
                     /locus_tag="VYO97_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754489.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            5092..5283
                     /locus_tag="VYO97_001728"
     CDS             5092..5283
                     /locus_tag="VYO97_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754490.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5637..5909)
                     /locus_tag="VYO97_001729"
     CDS             complement(5637..5909)
                     /locus_tag="VYO97_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1754491.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5968..6417)
                     /locus_tag="VYO97_001730"
     CDS             complement(5968..6417)
                     /locus_tag="VYO97_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1754492.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLINQPED"
     gene            complement(6404..8533)
                     /locus_tag="VYO97_001731"
     CDS             complement(6404..8533)
                     /locus_tag="VYO97_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1754493.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            complement(8635..8811)
                     /locus_tag="VYO97_001732"
     CDS             complement(8635..8811)
                     /locus_tag="VYO97_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEV1754494.1"
                     /translation="MDNLPYSNKLILVNRPYKNLKSQCVIPGQEHLPELAIMSDPLNT
                     FDIIAWLKKGGNAL"
BASE COUNT         2698 a         1826 c         1707 g         2587 t
ORIGIN      
        1 ttagaaatag attttactgt cctgatcgat ttgttctgtt cttgtttcat tttactatat
       61 gttcgtaaat gaaagttcta taatgaagtt agccacccag ccctcctaaa aattagatgg
      121 ttcaactagc tgatagctct tgtaggcata agaaaacacc tctgtgttat acttgttgtt
      181 caaccacaaa cacaagaaag gcacagagat gcaagacaat tatactacaa aagccaaaca
      241 tttgacaatc gatagccgtc gcttaatcga aagatggaaa aaagaaggga aatcaaatag
      301 agaaattgcc tctctacttg gaaaagctcc tcaaactatc cacactgaaa tcaagcgtgg
      361 gacagtccga caatgtcttg gaaaagggcg cttcaaagag gtttattctg ccgactatgc
      421 tcaacagtct tatgaaaaca atcgcaagcg ctcggtcaag aaatcaagct tgaccaagga
      481 actaaaggaa aagattctcc actatcataa ccaaaaattt tcgcctgaaa tgatggttat
      541 ggctaaaggg gttaacgtgg gaatttcaac catttactat tggattcatc atggaaaatt
      601 ggggttaagc aagcaggatt tgctttaccc tagaaaagga aaagcgctta agaaacaggc
      661 tagcaccaac tttaaacctg ctggtcaatc catcgaacag cggcctgaag ctatcaatct
      721 tcgcttggag aatgggcatt atgagattga tacggttcta cttacgagag cgaaaaacta
      781 ctgcttgctt gtcttgacgg atcgaaagag tagacatcag atcatccgat tgattccaaa
      841 taaaagtgct gaggtggtca atcaggctct aaaactcatc ttaaaacaac acaagattct
      901 ttccatcacg gcagataatg gaacggaatt caatcgcttg tttgatgtat tttctgagga
      961 gcacatctat tatgcgcacc cctatgcctc ttgggaaagg ggaactaatg agaatcacaa
     1021 caggctcatt cgtagatggt tacctaaggg aaccaagaaa atgactccca aagaagtcgc
     1081 attcatcgaa aagtggatta acaactatcc taaaaaatgc ttgaactaca agtcacccag
     1141 agaagacttc ttgatggcta acttgaactt gaaatttagc gtcagaaaca aatcacgaaa
     1201 tcgattcaaa ttttgcaggt cgtttaaata tcctgcgcgc aggtgttctt ggtgctaacg
     1261 atggaattat ttccattgct ggtgtggtta tcggggttgc cagtgccacg accaatatct
     1321 ggattatctt tttatcagga tttgcggcta tcttagctgg tgccttttca atggctggtg
     1381 gagaatatgt atccgtttca actccaaaag ataccgagga agctgccgtt tcgcgagaaa
     1441 aactcttgtt agaccaagat agggaactag ccaaaaaatc cctctatgct gcttatatcc
     1501 aaaatggaga atgcaaaact tctgcccaac tcttgaccaa taagatcttt cttaaaaatc
     1561 cactcaaggc tctggtagag gaaaaatatg ggattgagta tgaagaattt accaatcctt
     1621 ggcacgctgc catttctagc ttcgttgcct ttttccttag aagtttgcct ccaatgctgt
     1681 cagtgaccat attcccaagt gaatatcgca tccctgctac tgtccttatt gtcggtgtgg
     1741 cccttcttct cactggttac actagtgcca gacttggaaa ggatccgact agaacagcta
     1801 tgattcggaa ccttgctatt ggtctcttga ccatgggagt taccttcctg ctcgaacaac
     1861 ttttcagcat ttagaataca agaaatacct cgattttgaa gtcgaggtat cttttttaca
     1921 tttgcacaat cttgcgataa cttcttgaag taatcatgaa aatcagcaca taggcgatga
     1981 ggaagatagc gcagatagac aaggtcacaa tcaacatcat agtcgtatcc agtacaccaa
     2041 tcacttttaa aatcaggcta agcatatggt aggcaaaggc gagatgtatg aaggcaaaga
     2101 gcaaaggaag gaagaaaaca gttaaaacct gtttgttgat ggtttgcttg atttgctttt
     2161 ggtccaaacc gactttctgc aagataataa agcgttcacg gtcttcgtag ccttcagaaa
     2221 tttgtttgta gtagatgacc agaacagttc cgaccataaa gataatggat aggaaaatac
     2281 cgataaagaa gacaccacca aagagggcac tcatctgaga actagcatct gctagattgc
     2341 taccataaac atagctacct tctgtgtcta attgagcatt aaattgattg aggtagtttt
     2401 catactcctc agcgaccttg agttgttctt cttcgctggc atctacattc ataccaccgt
     2461 aaaactgatt atagatagcc gaatctggga attgatccaa aaaggcttgt aaatcaggaa
     2521 caacaaggta attgtaatca gtagtcaaga tattaaactt atttggaaca tggttgacaa
     2581 tgaaatcttt attaaattct tctttgacag aaaattgatg atcatttaga gttagagctt
     2641 tctgtccttt cagtccgtca tttttggcaa agagaccgac ctcatttcct gatagagaca
     2701 gtttttgacc agtcatattt tcataatctt tttggtcaaa taccatgaaa actgtttttg
     2761 gttggacacg gttttgtcct tttttaaaaa tagttaactt ggttccttct tgattcgcaa
     2821 cagcaaagta agtgtaacga agcacttctt tctctttaat cttataacct ttgtcacttg
     2881 caaactggct caagagtttg tccaaatctt ctttttcaac attttgccct gaaaccccaa
     2941 aatcatgagg atttagaact tttttaaagc tttctgagga attgaaaatg cttgtcgctg
     3001 ctgacatggt taccaaaacc attgttgaca agatagcgat ggtggctagt ccaactgcat
     3061 ttttcttcat acggaaaatc aagttagaaa cagatatgag attatttggt tggtaatagt
     3121 atttcttatt tttctttaag atttggagga aaacggtaat ccctgcatta aacaagagat
     3181 aagtcccaaa gataaccagt aaaacagcta tgaagaaggt tgttaaggct gtaagaggat
     3241 cttttaccgt aagggcaaga taatagccaa ttcctaaact tatggaacca agaatggttt
     3301 ggagaggaag gaagcgacct tttttctctc cactagcttt ctcacgagag agctggaggg
     3361 cattcatacg ggcgattcga agggcattca ggaacatgag gcctaggaaa atcaaaccga
     3421 agacaacaag cactgtaatg acaactttcg tctggaaggt agcaaccagc tcaaccttca
     3481 atttcattag tttgagcagg aaagcgaaaa ttaacttgtc aaacaaggct ccaataccga
     3541 tacccgctcc aacagttaga atcccaaata ccactaactc cttaaaggtc atactgataa
     3601 gatgacgctt ctccaagccc aacatgccat aaattcctag ttccttggaa cggttcttca
     3661 tgacaaaact attggcatag agaacgataa tggctgacgc aagggtgacg acaaacatac
     3721 caaatccaag tgtagcctga atggttgttc ctccacggat ttccgcaatc ttaggattga
     3781 aggttagaga ataaaagaga taggtgacag tgactgccaa gagaacagcc agcgcaaaag
     3841 gatagtagag tttgcggttt ttaatcaagt tcgataccgc taacttattg gttaatcgaa
     3901 acatactaat tcacctcgct tgccatgaca gtcaaggtat cagagatttc ttggaacatc
     3961 tgacgttctg tcttctctcc acggtagatt tgattgtaaa gaatgccgtc tttgataaag
     4021 agtacacgtt tagctctgct agctgctgct gttgagtggg ttaccatgag aatggtttgg
     4081 ccgcgctcat tgatttcatc aaatacatca agtaaggctg cagatgactt ggagtcaaga
     4141 gctcctgtcg gttcgtccgc aaggagaatt tcaggttcag tgatgatggc gcgtgctact
     4201 gctacacgct gtttctggcc accagaaatc tcgtaagggt acttctcttg taattggttt
     4261 attcccagat tctcagctgt caccaccaat ttcttcatca tctctgtaat aggtcttctt
     4321 gacaagacaa gcggaagcaa gatattgtcc tttacagata gagtatctag caagttaaag
     4381 tcttggaaga caaagcccaa cttttcacgc cggaaactag aagcctgtga atttttaata
     4441 gttgctgtat cagttccatt taagtaaacc tgaccacgac ttggtttgtc cagcatagct
     4501 aggatattga gaagagtgga tttaccagaa ccagactcac ccatgatggc aacatagtca
     4561 cccttttcta cggtaaagtg aatatccttg agggcttcta cttggttgcc ctgaaaacgt
     4621 gttttataaa ttttttgaac gtgttttaca tctaaaagtg tcatgagaat ctcctttctt
     4681 aatatcccat aaaatgaaat gtagcttgca tcatagtgca tcccagctga acgcgctcaa
     4741 tttctttgtg acttggtttt ctagttttct tatgtacgat ttgtttcatg atgttactcc
     4801 tttgttcttt atgagtctag tttacatcaa aaaaagaccg cttgccataa cctaacctta
     4861 caaaagagac tttaatctta catttttgta agattggggg aaatataagc aaaattactt
     4921 agaaaatgat ttgatttcct atttttaaag ttgtaaaata taagtgcgaa aaggcttact
     4981 ttgaaaagga ggaagcggga actaacattt tagtaaggtt acctagatga cacaccatta
     5041 aattgaacac tttcaaatcg aactacctat aaggaaagaa ggtgcgcttt aatgaaacat
     5101 aaagaacata tactcatcgg atttctctat ctgctctctc cattcatcgg tcagctctta
     5161 gttgaacaca cacactttat cggtacagaa tttaccggca ctgcttatgt tatttgctgg
     5221 ttatcagttg ttatcagcat ccaccatttt tccaaaaatg tattgtctca gcagcaaaaa
     5281 tagattaaaa aactgcattt aaattactgc ttttatagta aaatgaaata agaacaggac
     5341 aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat
     5401 tctagttcca atccactata gtttgtagat gaagctggcg aaatattatt aatactattc
     5461 aaaaatcaaa gtgcaaacta tatcaacttc acattgccga ctaaattaca gcctgcggct
     5521 ggcttcctag tttgcacttt gatttttatt gagtataaaa gtaaaaaagc tttaaatcaa
     5581 gcagtttaga ctattgattt aaagcttttt gcgtattcca aatcttttgt aaaacctcac
     5641 caaaaccagc cttcattatc gtcaatggct tgagcttctt tgcgtttacg tgggcctgtt
     5701 ccgtacattt ctgcttcgat ttcttccttg gttggcatga tagaagctag gatgatatag
     5761 ataatcaccc caagtccaaa gtttgcgact gtaaaaatgg caaacagaaa acgtacaagg
     5821 gtaacatcaa agttccactt gtctgacaga cctgccaaaa ctcctgaaat catgcgattt
     5881 cgtctcattt tataaaattt actgttcatg atatttcctc tttctgctag gttagacata
     5941 gtatatcacg ggtaggctcg gctttcatca gtcctcaggt tgatttatta gtagcaattt
     6001 acctcgacaa agtccacagc gatagcgttg ggtatcaatc ctacgcttac gctgataagt
     6061 ttgctggcag gattggcaac gatagagctt gattggcttg gagttactat tgggcaagga
     6121 tggtacaaac cgtaatccat ctactgcttt caacagttcc ttaaaatccc gatccttgtg
     6181 ttgatagcct ttcccttgaa aatagaggtg ataatgacag agttcatgtc ggacaatttt
     6241 cctaaaaacg tccaacccca gttcctgata aaccttggga ttaaaatcca aatgcccatc
     6301 tttggggaaa aatcgcccac ctgtcgaacg tagacgccta ttccactgga catgatggat
     6361 aaaaggtctg ccgaagtctt ctagtgaaac ctgcttgacg taatcagtca gtttcatttg
     6421 gagctaggag agacagatta actttttcac gttcagtatc aattttctta acccaaacgc
     6481 tcaccaaatc tccaactgcc accacttgac tagggtgttt gataaacttg cgactcatat
     6541 gggaaatatg gatgagaccg tcctcatgaa ttccgatatc aacaaaagca ccgaaatcaa
     6601 caacgttacg caccactcct tctagctttt gtccaaccac taagtccttg atatctagga
     6661 catcttgacg gagaactgga gcatcaaagg aatcacggaa atctcgacct ggtttgagaa
     6721 gatctgcaat gatatcttta agagtttctg gaccaaggtc taactcttgc gccatttcct
     6781 tgactgaaag cgacttgagt ttactttggg cctcttcatt cagatcctta atgtccaagc
     6841 atttgaagag ttccttaacg gcagcgtaat tttctgggtg aactcctgta ttatcaagga
     6901 tattgctact ttcagggata cggaggaaac cagcagcctg ttcaaaagcc ttggccccca
     6961 aacgaggaac tttcttgatt tgggagcgtg aagtgatttt tccctcttcc tcacggtatt
     7021 tgacgatatt ttcagaaatt gttttattga gtccagccac gtgagaaaga agagctgggc
     7081 tagctgtatt aacattgaca ccgacttggt taaccaccgt atccacgaca aagtccagac
     7141 tctcagatag tttcttctga ctgacatcgt gttggtattg accgacacca attgacttag
     7201 gatcgatttt gaccaattcc gcaagaggat cttgcaaacg acgggcgata gaaatggcag
     7261 agcgtttttc aacggtcaag tctggaaact cctgacgagc aagttcgctg gcagaataga
     7321 cagaagcacc actttcatta acgataacat agctgacttc agggaaatct ttcagaactt
     7381 ccgctacaaa agcttcgctt tcacgactgg ccgttccatt tccaatggca ataatctcta
     7441 caccgtattg accaattaaa tctgctaaat ctttcttggc ttcttcgatt tgacgagctg
     7501 atgctggttt aacaggataa ataacctgag ttgtcagcat ttttcctgtt gcatccacga
     7561 cagctagctt ggcacctgta cgaaaggctg ggtcaaaccc aagaaccacg cgccctttca
     7621 gtggagcaac caagaggaga ttgcgcagat tgtcagaaaa aagttggata gctccctctt
     7681 cagctttctc agttaattct gtccgaatac gacgctcaat agcaggcaag acctttttct
     7741 taacggattg ctgaacaact tcatcaatat aagcattttt caccttgaaa cgagtagcaa
     7801 agaaggcaag aatacggtcc gtcgcatgtt caaaaccgat cttcaagaca ccaagtttct
     7861 ccccacgatt gagagccaag gtacgatagc cttgcatagt tccaactgtc tctgaaaaat
     7921 cataataaat ctgaaaaacc tgcttttcat caagactttc atccttggct tgagaagtga
     7981 gtttagagtg tctcagcact tcctgataag tcatagaacg caaggtcaca tcttccgata
     8041 aggcttcgac caaaatatca actgcaccgg tcaaggcttc cttgccagtc gcaaatcctt
     8101 cacagacgaa cttttcagct tctttctcta agtcaactat attctgcaaa atcaagcgag
     8161 caagaggaaa gagtccagct tcacgggcaa tggttgcctt ggtacgacgc ttttccttat
     8221 aaggaagata gagttcttca acgtctgcta atttttcggc aactaagata gcttcttcca
     8281 attccttggt caacttacct tgttcttgaa tcttagctaa gacagcttcc ttacggtcat
     8341 tgagatttgt cagactttta tccaaatcaa taatagcctt aatcgccacc tcatccagac
     8401 taccagtcat gtccttgcga taacgcgcga taaagggaat agtcgcccct tcagctgtca
     8461 aacttagaac ggtatcaatt tgctttaacg tcactcccaa atcctgagag attttttcat
     8521 attttttatc cataaatcta ttataccaca agctaaacgt ttcaaattaa ctcgtagaac
     8581 atttaaaaaa tatgtaggaa atagatttat atgctacagc gcaataactt gcacttaaag
     8641 agcattgcca ccttttttta accaagctat gatatcaaaa gtatttaatg gatcagacat
     8701 aatagccagt tctggaagat gttcctgacc tggaataaca cattgacttt tcaaattttt
     8761 atatggacga ttgactaaaa ttaatttatt agaataagga agattatcca tcttattt
//
LOCUS       DAVMAF010000075         8813 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMAF010000075 DAVMAF010000000
VERSION     DAVMAF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.076"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            24..179
                     /locus_tag="VYO97_001733"
                     /pseudo
     CDS             24..179
                     /locus_tag="VYO97_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            379..3654
                     /gene="rexB"
                     /locus_tag="VYO97_001734"
     CDS             379..3654
                     /gene="rexB"
                     /locus_tag="VYO97_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1754495.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LEILFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLEQQGLENPAIPTSPSSKTLAKDTLQALY
                     PDKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3651..7301
                     /gene="addA"
                     /locus_tag="VYO97_001735"
     CDS             3651..7301
                     /gene="addA"
                     /locus_tag="VYO97_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1754496.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGIEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIDSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEAYRQCKRQENAFEFTDIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMNILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWVISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7516..8694
                     /locus_tag="VYO97_001736"
     CDS             7516..8694
                     /locus_tag="VYO97_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754497.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSNFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2727 a         1852 c         1875 g         2359 t
ORIGIN      
        1 tatagttcaa tcattctatc aagaatattg aatatataaa ggcacgtaat attaaaactt
       61 cttctgatat cgaattagag tccgatgttg atggcgataa atcagacaat ctacccgtcg
      121 acattaagat actgggacaa cacattgaag tgtattccgt acctaagaaa caatcataaa
      181 actcaaagcc ttgttaccga ggctttttta ttatgtgatt ttcagtagcc agacttattc
      241 tatctaatac aagtaaagct aagtatttaa gatttagcag atgatgaaga aaagaaagga
      301 tggcctaaat attttaacag atttacgagg ctaatcatgg tataatatag ggtgatgaaa
      361 aattccaacg aggctgagat gaaattactt tatactgata ttcgaacttc tttgacagaa
      421 attctaacaa gagaggcaga agagctagtt gcagctggca agcgggtctt ctacattgcc
      481 cccaactctc tttcttttga aaaggaacgc gccgtgctgg aatgcttgtc ccagcaggct
      541 tctttttcga ttaccgtcac gcgctttgct caaatggctc gttatctggt cttgaatgat
      601 ttaccagcta aaactactct tgatgatatc ggtcttgggt tggcctttta caaatgcctt
      661 gccgaactcg atcccaagga cttgcgtgtt tatggcgcta ttaagcagga tcctcaattg
      721 atccagcagt taattgagct ttaccatgag atgaccaaat ctcagatgaa ttttttggac
      781 ttggagaatt taacagatga ggataagagg gcggatttac tcttgatttt tgagaaagta
      841 acagcctatc ttaatcaagg gcagttagcc cagggaagtc agttgtccca tttgattgag
      901 gctattgaga atgacaaggt aagtagtgat tttaatcaaa tcgccttggt cattgacggc
      961 tttactcgtt tttctgctga ggaagagcgg gttgtggact tacttcacgg caaaggtgct
     1021 gagattgtta tcggggctta tgctagtaag aaagcctata ccagtccttt tagcgagggc
     1081 aatctctacc aagccagcgt aaaatttctc catcatctgg cttctaaata ccaaacgccc
     1141 gctcaggact gttctcaaac tcatgagaag atggatagtt ttgacaaggc ctctcgtttg
     1201 ttggagtctt cttatgactt ttcagaactc gctttggatg tcgatgagaa agaccgtgaa
     1261 aatttacaaa tctggtcttg tttgacgcaa aaggaggagt tggagctagt agcccgtagc
     1321 attcgtcaga aattacatga gaactcagac ctgagctaca agcattttcg tattctcttg
     1381 ggggatgtag cttcttacca gttatctctc aaaaccattt ttgaccagta tcagattcct
     1441 ttttatcttg gtagaagcga agccatggct catcatccct tgactcagtt tgtcgagtct
     1501 attttagctt taaaacgtta ccgttttcgt caggaggatt tgattaatct tcttagaact
     1561 gacttgtata ttgacctcag tcagtctgat attgatgctt ttgagcaata tatccgctat
     1621 cttggtatca atggcttgcc agcctttcag caaaccttca ccaaatccca ccatggaaaa
     1681 tttaatcttg agcgtttgaa tgtcctccgc ctgagaattt tagcacctct tgaaatcctc
     1741 tttgccagcc gaaaacaaaa ggctgaaaaa ctcctacaaa aatggagtgt ctttctaaaa
     1801 gaaggagctg tgaccaagca gttacaagat ttgacaacca ctttggaagc tgtagaacag
     1861 gaaagacaag ccgaagtttg gaaggctttc tgccatgttt tagaacaatt tgcgactgtt
     1921 tttgctggtt cacaggttag tctggaagac ttcctagcct tgctccattc tggaatgagt
     1981 ttgtcccaat accgtaccat tccagcaaca gtggacactg ttctggtgca gagttacgat
     2041 ttgattgcac cattgactgc tgactttgtc tatgctattg gactaactca ggacaattta
     2101 ccaaaaattt ctcaaaacac cagtcttctg acagatgaag aaaggcaaaa tctaaaccaa
     2161 gcgaccgaag aaggcgttca attactgatt gccagcagtg aaaatctcaa gaaaaatcgc
     2221 tacactatgc tttccttggt caattctgct cgtaagcagt tgttcttgtc ggctccaagc
     2281 ctttttaacg aaagtgaaag taaggaatct gcctatcttc aagagttgat ccattttgga
     2341 tttaggcgga gagagaagag gatgaatcac aaaggactgt ctaaggagga tatggggtcc
     2401 tatcacagtc ttttgtctag tctggttgcc tatcaccagc agggtgagat gagcgatact
     2461 gagcaagatt tgacttttgt caaggttttg tcgcgtgtta taggtaaaaa actagagcag
     2521 caaggtctgg aaaatccagc catcccaacc agtccaagca gcaagacctt agccaaggac
     2581 accttgcaag ctctctatcc tgacaaacag gagttttacc tgtctacgtc gggtttgaca
     2641 gagttttatc gcaatgaata cagttatttc ctacgctacg ttttaggctt gcaggaggaa
     2701 ttacgtttgc gtcctgatgc ccgtagtcac gggaatttct tgcatcgtat ctttgaacgc
     2761 gccttgcagt tgcctaatga agattccttt gaccaacgtc tagaacaagc tattcaagaa
     2821 accagtcaag aacgcgaatt tgaagctatt tatcaagaaa gtttggaagc ccagtttacc
     2881 aaggaagttt tgcttgatgt tgcacggaca actggacata ttctccgaca caatccagcc
     2941 atcgaaacca tcaaagaaga agcaaatttt ggtggaaaag accaagcctt tattcaatta
     3001 gacaatggac gcagtgtctt tgtacgaggc aaggtggacc ggattgaccg tttgaaagct
     3061 aatggagcga taggagtagt agactacaaa tccagtctga ctcagttcca gtttcctcat
     3121 ttctttaatg ggctcaattc tcagttacca acctatcttg ctgccctaaa aagagaaggg
     3181 gagcagaact ttttcggcgc catgtacttg gaaatggctg aacctgtcca atctctgatg
     3241 gcggtaaaaa gtctggcagg agcagtggta gaagccagca aatctatgaa ataccaaggg
     3301 ctcttcttgg aaaaagaaag cagttattta ggcgaatttt ataacaaaaa caaggctaat
     3361 caactgacag atgaggaatt tcagctccta ctggactaca atgcctatct ttacaagaaa
     3421 gctgctgaga agattttagc aggccggttc gccatcaatc cttatactga aaatggcaga
     3481 agcattgccc catacgtcca gcaacatcag gctattacag gctttgaagc caattaccat
     3541 ctgggccaag cccgtttcct agaaaagttg gacctagctg atggcaagcg tctggtcgga
     3601 gaaaaactca agcaagcttg gcttgaaaaa ataagagagg agttgaatcg atgaagctta
     3661 ttcccttttt aagtgaggag gagattcaaa aactgcaaga agcagaagca aattcgagca
     3721 aagaacagaa gaaaactgcc gagcaaatcg aagctatcta cacttctggt cagaatatcc
     3781 tggtctcagc atcggctggt tctggaaaga cctttgtcat ggcagagcgc attctggacc
     3841 aattggcgcg tggtatcgaa atttctcaac tctttatctc aacctttacc gtcaaggctg
     3901 caactgaact taaagaacgt ttagagaaaa aaatcagcaa gaaaatccaa gaaacagatg
     3961 atgtcgacct caaacaacac ttgggtcgcc agttggcaga cctacccaac gctgccatcg
     4021 gaaccatgga ctctttcaca caaaaattcc ttggcaaaca tggttatctg cttgatattg
     4081 cacctaattt ccgtatttta caaaaccaaa gcgagcaact tctgctcgaa aacgaagtct
     4141 ttcatgaggt ctttgaagcg cattaccaag gtaaacagaa agagaccttt agtcatttgc
     4201 tgaaaaactt tgctgggcgt ggcaaggacg aacggggtct gcgccagcag gtctataaaa
     4261 tctatgactt cctccaatcc accagtaatc ctcaaaagtg gctgagtgaa tctttcctca
     4321 aaggatttga gaaagctgat tttaccagtg aaaaagaaaa actgactgag cagatcaaac
     4381 aagccctttg ggatttggaa agctttttcc gttaccatct ggataacgat gccaaggagt
     4441 ttgcaaaggc tgcctattta gaaaatgttc agttaattct ggatgaaatt gactccctaa
     4501 atcaggagtc cgatagtcag gcttatcagg cagtgcttgc gcgtgttgtc gccatctcta
     4561 aggagaaaaa cggtcgagct ctgactaatg ccagtcgtaa ggctgatttg aagcccctgg
     4621 ctgatgccta caacgaagag agaaagaccc agtttgctaa actaggacaa ttatcagacc
     4681 agataaccat tctcgactat caagaacgtt atcatcaaga cacatgggaa ctagctaaaa
     4741 ccttccaaac ctttatgagt cattttgtgg aggcttatcg tcagtgcaaa cgccaggaaa
     4801 atgcctttga attcactgat atcagccatt acaccattga gattttagag aatttcccac
     4861 aagttcgtga gtcttatcag gagcgcttcc atgaagtcat ggtcgatgag tatcaggata
     4921 ccaaccatat tcaagaacgg atgctggaat tgttgtctaa tggccacaat cgctttatgg
     4981 tgggagatat caagcaatcc atctatcgtt tccgtcaggc agacccgcag attttcaatg
     5041 agaaattcca acgctatgcg caaaatcccc aagaaggcaa gctcattctc ctcaaggaaa
     5101 atttccgtag tagttcagaa gtgctgtcag caaccaatga tgtctttgaa cgtctcatgg
     5161 accaagaggt cggcgaaatc aactatgata acaagcacca gcttgttttt gccaatacca
     5221 aactgactcc caatccagac aacaaggcag aatttctcct ctacgacaag gacgatacag
     5281 gtgaggaaga agagagtcaa acagaaacga aactaacagg cgaaatgcgc ttagttatca
     5341 aggagattct gaaacttcat caagaaaaag gtgttgcctt taaggaaatt gcccttctga
     5401 cctccagccg cagtcgtaat gaccagattc tcctcgccct gtctgagtac gggattcctg
     5461 tcaaaactga cggagagcaa aacaattatc tccaatccct agaagtgcaa gtcatgctag
     5521 acactcttcg tgtcattcac aatcccctgc aagactacgc cttggttgcc cttatgaagt
     5581 ctccaatgtt tggttttgat gaggatgagc tagcacgttt gtcccttcag aaagcagagg
     5641 ataaagtcca cgaaaatctc tatgagaaac tggtcaatgc acaaaaaatg gcaagtagtc
     5701 aaaaaggctt gattcacaca gctctagctg aaaaactaaa gcaattcatg aatatcctag
     5761 cttcttggcg cttgtatgcc aaaacccact ctctctatga cttgatttgg aagatttaca
     5821 acgaccgttt ttattatgac tatgttgggg ctttgccgaa tggtcctgct aggcaggcca
     5881 atctctatgc cctagcactg cgtgctgatc aatttgaaaa gagcaatttc aaaggtttgt
     5941 cgcgttttat tcgtatgatt gaccaagtct tagaagccca gcacgatttg gcaagcgtgg
     6001 ccgtcgcacc gccaaaagat gcagtagagc tcatgaccat ccacaagagt aaagggctgg
     6061 agtttcctta cgtctttatc ctcaatatgg atcaagattt caacaagcaa gactctatgt
     6121 cagaagtcat tctcagtcgt cagaatggtc ttggtgtcaa atatattgcc aagatggaga
     6181 caggggcagt agaagaccac tatcctaaaa ccatcaaact ctccattcct agtctgacct
     6241 ataggcagaa cgaagaggaa ttacagctag caagctattc tgagcagatg cgtttgctgt
     6301 atgttgctat gacgcgggct gagaaaaagc tctatcttgt cggcaagggt tctcgtgaaa
     6361 agctggaatc caaggaatac ccagcagcca aaaatgggaa actaaatagc aatactagac
     6421 tgcaagcacg gaatttccaa gattggcttt gggttatcag taaagtgttt actaaggaca
     6481 agctcaactt tagttatcgt tttattggcg aagatcagtt gaccagagaa gctatcggag
     6541 agttggaaac caagagtcct ctccaagata gctcccaagc agacaatcgt cagtcagata
     6601 ccatcaaaga agctctggaa atgctgaagg aggtggaagt ttataatact cttcaccgcg
     6661 cagctattga acttcctagt gttcaaaccc caagtcaaat caagaaattc tacgaaccag
     6721 ttatggatat ggaaggtgtc gagattgctg gtcaaggtca gtcagtaggc aagaaaatca
     6781 gcttcgattt gccagatttt tcaaccaaag aaaaggtaac tggagctgag attggtagtg
     6841 ctactcacga actcatgcag agaattgacc tcagccagca actaaccctt gctagcctaa
     6901 cagaaacact caaacaagtt caaactagcc aagctgtcag agacaagatc aatcttgata
     6961 aaattcttgc tttctttgac acagtactcg gtcaggaaat tcttgctaat accgaccatc
     7021 tttatcgcga gcaacctttc tccatgctca aacgagacca aaagagtcag gaagactttg
     7081 ttgtccgtgg tatccttgat ggctatctgc tttacgaaaa caaaattgtt ctgttcgact
     7141 acaagacaga ccgctatgat gaaccaagtc aactcgtaga ccgctatcgt ggtcagttag
     7201 ctctatacga agaggcttta tcacgagcct attcgattga aaatattgaa aaatacttga
     7261 ttttactcgg taaagacgag gttcaagttg taaaagtata acttatactc tttgaaaatc
     7321 tcttcaaacc acgtcagctt cgccttgctg tatatatggt tactgacttc gtcagttcta
     7381 tccacaacct caaaacagtg ttttgagctg acttcgtcag ttctatctac aacctcaaaa
     7441 cagtgttttg agctgactgc ggctagcttt ctagtttgct ctttgatttt tattgagtat
     7501 tagaaaggag acctcatgac acttccagtt agaaaatccc tgcacgatgc agttttacag
     7561 gcttcgaaag cggatacttg ggaacaagct accaaggaat ggaatgaagt ttcctttatt
     7621 tttaacggta tcggccgtag caattgtgtc tgtggaaatg ccatcaaata cgcttatgaa
     7681 ctctttaacg gtgtcacagg ccaacgcctc tttccaattg gtagcgactg tgttcgtcat
     7741 tttcatcgat tgtcccttga ccagcaattg gaagaggaag aaaaactgct cagaaaggtt
     7801 gaaaatctaa ccagaaaagc tcagaaaaag gaaaaaatca aggtcaataa aagtaacttt
     7861 gacgagcgac ttctaaaatg gctctgggaa aaaggtgttt tcaaacccaa tcgtggcaat
     7921 caatttgcgc ctgagagaga ctaccagctt ttcctagaag tctttcaggg aggaagttgg
     7981 accaaggcgg aaccaaagaa aaaggctcgg atggaagaag tccttgaaaa gtgtatcaaa
     8041 ccctttttac ttgggaaatc ggatgaccaa ctctaccttg tcaagctagg caaggagaaa
     8101 attgactacg aacaagaact ccgtatccag gcagagaaag aacgcaagaa gagggataaa
     8161 atcgccaagc aatatgcaga caatctggtt ctagccatgg gacccgcaga gcgtgcttat
     8221 caagattact ttggctttac agagaccctg actcaagaag aacgcaagtg ggaaaaaatt
     8281 ctttttggta aaaatagagc agaacgagct atcaaggcca aacaatacca aaaagagctg
     8341 gaaaaagacc aacgaattgc cagtcaggat ccaattgaaa gaaaacagaa gcagacttgg
     8401 atcctcaatt cctattttcg tgagcttcct gaagaaaaag ctcgattttc tagactctta
     8461 ttagaatatc gaaaaagtgg tgaagtaccc ttttcaactg aatacctgtc agatcatctc
     8521 atcgactttt tctataaaat gaaagccttt gaatttgaaa tcgcaccaga acaagtccga
     8581 gattttctaa aaaaaagcct gcaggaagac catcgctcat cagcacaagg aagctggata
     8641 gaaggaattc tcctcaactg cctcaaacca tttttagaac gattagtgat atgagtaaca
     8701 cctaccgtgt aaatttcatg gtaggtgtta ttgtaccccc aaataatata aaacaaagta
     8761 tgaaagcgca tctcaatttt aagacttttt tgtaaattaa gagtataatt aag
//
LOCUS       DAVMAF010000076         8656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMAF010000076 DAVMAF010000000
VERSION     DAVMAF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.077"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            5..3022
                     /locus_tag="VYO97_001737"
     CDS             5..3022
                     /locus_tag="VYO97_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV1754498.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLIIKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     DSYDQPSLNFDQKNHMVNITINSNGNLEFELHF"
     gene            3037..3765
                     /locus_tag="VYO97_001738"
     CDS             3037..3765
                     /locus_tag="VYO97_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV1754499.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVQSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            3778..5544
                     /locus_tag="VYO97_001739"
     CDS             3778..5544
                     /locus_tag="VYO97_001739"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1754500.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            5690..6841
                     /locus_tag="VYO97_001740"
     CDS             5690..6841
                     /locus_tag="VYO97_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1754501.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            6985..7869
                     /locus_tag="VYO97_001741"
     CDS             6985..7869
                     /locus_tag="VYO97_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1754502.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            8243..8494
                     /locus_tag="VYO97_001742"
     CDS             8243..8494
                     /locus_tag="VYO97_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1754503.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            8482..>8656
                     /locus_tag="VYO97_001743"
     CDS             8482..>8656
                     /locus_tag="VYO97_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754504.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEDKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKL"
BASE COUNT         2877 a         1396 c         1848 g         2535 t
ORIGIN      
        1 aaaaatggaa agtaaaaaaa tagcaaaaca gattttaata gctacggctg ttttaacaag
       61 ttttttagga agtaatttgg tttatgcaga tgtagttcaa agtaattcta ataacagagc
      121 tagtactgaa actgctagag taacagggaa taacttagaa aaacttatta ttaaagataa
      181 agaaattgat aaagagatga catatctttc agatatggat tggtcttctg caactcatgg
      241 tgatatagat aaaacaaaaa cagtacaaaa agatgctcca tttacaacag gaaataaagg
      301 agagcatact aagatttcat tgctcacttc tgatgataaa gttaaatatt ttgataaagg
      361 aataggaaca gtcgcagatt ctccatcagt aatcagctat gatatcagcg gtcaagggtt
      421 tgaaaaattt gaaacttata taggaattga tcaaagtgct aatagttcta gatcagacca
      481 tgcagtagtg gatagaatag aaatagaaat tgatggaaag gttgtctatt ctagtagtgt
      541 aactaatcca gaaggattca gatataatac ccaggcacaa tttattagtg tgacaattcc
      601 acaaaacgct aaaaaaatat ccttaaaatc attcgcagga gaacatacat ggggtgatga
      661 ggttgtattt gctgatgcta aacttattaa aactgttagt actcaaacta taactccaga
      721 tcttcttaat aaaggaatta atggaggagt atatctttct gatttagaat gggttgatgc
      781 tactcatggt gatgacgata aatcaaaaac agttcagaaa gataaaccgt ttacacctgg
      841 taataacggg tctaataata aaataaaatt actaattgac ggtaaggaag tcgaatttaa
      901 taagggattg ggaactgtag caagtaaccc gtctagtata aaatatgatg tttcgggtgc
      961 taatgttaca agatttattt cctatgtcgg aattgatagg agtgcaaatc atttaaattc
     1021 agattacgca gatattcaaa aatttgaagt tgttgcggat ggtaaagtca tttactcatc
     1081 agattcaaaa tatccaaaag gaattaagta tgatacatcg gcatttctgg ttgatgttga
     1141 aattcccaaa gatacacaga caatagaact taaatcatat tcagggaaac atacatgggc
     1201 tgacgaattg gttttaggtg gagccttgtt tatggcaaat ggtaaattta agaatcctaa
     1261 tgattggtca gaagttgata aacgtcgtga aattaataat gaacatccgt tacttatgat
     1321 gcctttatat gctaacggag aagagtttaa ccaagggaaa tatacttttt ggggaggaga
     1381 tacattaaca ggtaagtggg agaatatacc agatgactta aaaccatata ctgttataca
     1441 gttacatcca gatgatcttc ctaaaagaga tggagctgct agagattttt atgagcatat
     1501 gttagaagaa gcagcgaaat atgtaaatcc taagactgga aaaaatgaac ctattccagt
     1561 tattctaacg gtttacactg ctggtaatat gccttattat acttcggcac attggttaag
     1621 taccagctgg attgataaaa tgtatcaaaa atatccaaat ttgcatggta ttttcagtac
     1681 tgagaattat tggatttggg caaatgatat tgaaaataag gcggcagatt acttaaaagt
     1741 atcagctaaa aatggaggat attttatatg ggctgaacaa aataatggtt ctgcaataga
     1801 aaaagcattt ggaaaaaacg gtaagatagc atttcagaaa tctgttgata agtactggaa
     1861 gaaccttatt ttcatgttca aaaatacacc tgctgctgag ggaaatgatt caactacaga
     1921 aagttatatg aagggacttt ggctttcaaa tcatacttat caatggggtg gtttgatgga
     1981 tacttggaag tggtatgaga caggtaaatg gaaactgttt gcaagtggta atatcggaaa
     2041 aagtcaagga gatagacaat ggttgactga accagagtct atgctaggag aagaagcttt
     2101 aggggtttac cttaatggag gtgtagttta taattttgaa cacccagctt atacctatgg
     2161 tgtaaataat aaagaaagct tactctttag cgaagtaata aaagaatttt ttagatatgt
     2221 aatagcgcat ccagcaccat ccaaagaaaa ggtacttgag gatacaaaag tattcataca
     2281 tggagactat tcaaataaag gaaatggtaa gttctttgta aacgtaaata ctgatagaga
     2341 acaaactcca ttatacatga ctggtcgtta taatgttatt ccagctattc caggtgtttt
     2401 aaaaacagat aaattgaaag agtctgtttc tggtagccgt attcagatta aggaaatcac
     2461 ttctccagaa tttagctcta ctcaggctag aaaagaatac ttaaataagc tgtatcctat
     2521 gaattatgaa ggagatatct ttgctcagaa attagataat agatggtttg tctataatta
     2581 taaggtaaat gagaatgtta aacagactgg gaaattaaaa tttaattctt tagaaatgga
     2641 tgttgaattt gaacctcata cctatggtat ttttgagaga ataagtaatg gattgaaagt
     2701 gaatttaaat aattttagaa caaataaaga ttcactatgg tctaatgctc aagatgcaaa
     2761 tcaggcaaag aaactaccac aattgaccaa gaaaggtgcc attaagtgga ttgaagaaca
     2821 ttatatcaaa gatactcagt ttggagagaa aagggttaca aagatagtat taagaggtat
     2881 agataaactt cctactattc attcattatc aggaacaaat gattcctatg atcagccatc
     2941 tctcaatttt gatcagaaaa atcatatggt taatattacc attaacagta acggaaatct
     3001 tgaattcgaa cttcattttt agaaaggagt gagtttatgt ctcaagatga aaaattaatt
     3061 cgtgaacaga tttgtgatgt ttgtcataag atgtggcaac ttggttgggt tgctgctaac
     3121 gatgggaatg tatctgttcg attagatgag gataccattc ttgcaacacc tactggtatc
     3181 agcaaaagtt ttattacacc agaaaagctg gtgaagttaa atcttaaagg agagatttta
     3241 gaagcagaag gtgattactg tccttctagt gaaattaaaa tgcacattcg gtgctacgaa
     3301 gaacgtgagg atgttcgttc agttgttcac gcgcatccac cgattgcaac aggatttgct
     3361 cttgcacaca ttcctttaga tacttattca ctaattgaga gcgcgattgt ggtaggggca
     3421 attcctataa cgccatttgg agttcagtct acaatggaag tgccagaagc aattacacct
     3481 tatctgcccg atcatgatgt catgctatta gaaaatcatg gagctctgac tgtcggaagc
     3541 gatgtaatta cagcatacta ccgtatggaa actttagaat tagtcgcaaa gacaaccttc
     3601 cacggaagaa tgttactttc tacaaagggc attgaggagc aagaaattgc tcgtccgact
     3661 ttagaacgtc tattctcaat gcgagaaaat tataaggtta caggtcgtca cccaggctac
     3721 cgtaaatata atggcgatgg tagtatgaaa gaaacagaaa aataagagga aaatagtatg
     3781 attcaacatc cacgtattgg tattcgtccg actattgatg gtcgtcgtca aggtgtacgc
     3841 gaatcacttg aagtacaaac aatgaacatg gctaaaagtg tggcagattt gatttcaagc
     3901 acattgaaat atccagatgg ggaacctgtg gaatgtgtga tttctccatc taccattggt
     3961 cgtgttccag aggctgcagc ttcccatgag ttgtttaaaa aatcaaatgt ttgcgcaaca
     4021 attacagtta caccatgctg gtgttatggt agtgaaacta tggatatgtc tccagatatt
     4081 cctcatgcta tttggggatt taatgggaca gaacgcccag gagctgtcta tcttgcagct
     4141 gtactagctt cacatgctca aaaagggatt ccagcctttg ggatttatgg tagagatgtt
     4201 caggaagcta atgatacagc tattccagaa gatgtcaaag aaaaactttt acgttatgcg
     4261 cgtgcagctc ttgcaactgg cttgatgaga gacactgctt acctatcaat gggtagtgtt
     4321 tcgatgggga ttggtggttc tattgtaaat ccggatttct tccaagaata cttaggaatg
     4381 cgaaatgaat cggtagatat gacggagttc acacgccgta tggaccgtgg tatttacgac
     4441 cctgaagagt ttgaacgtgc tatggtttgg gtaaaagaac atatcaaaga aggagttgac
     4501 cgaaatcgtg aggatttgat tctttctaaa gaagagaaag aaaaacaatg ggaatttgtt
     4561 attaagatgt tcatgattgg acgtgactta atggttggta acccaagact tgctgaactt
     4621 ggttttgagg aagaagcagt tggtcaccat gctttagtag ctggtttcca aggtcaacgt
     4681 cagtggacag accattttcc aaatggagat tttatggaaa ctttcctcaa tactcagttt
     4741 gactggaatg gtattcgaaa accatttgta tttgcgacag agaatgattc actaaatggt
     4801 gtgtctatgc tctttaatta tctattaaca aatactccac aaatctttgc tgatgtgcgt
     4861 acttattgga gtccagaggc tgttgaacgt gtaacaggac atactttaga gggtcgtgct
     4921 gcagctggct tcttacatct aatcaactct ggatcttgta cattggatgg tacaggtcaa
     4981 gctactcgag atggcaaacc tgttatgaaa ccattctggg agttggaaga aagtgaagtg
     5041 caggctatgc ttgaaaatac agacttccca ccagcaaacc gcgaatactt ccgtggagga
     5101 ggattctcaa ctcgtttctt gacgaagggg gatatgccag taacaatggt acgtctcaat
     5161 cttctaaaag gggttggtcc agtgctacaa attgcagaag gttacacact tgaacttcct
     5221 gaagatgttc accatacttt agataatcgt acagatccag gatggccaac tacttggttt
     5281 gctccacgtt tgacaggaaa aggtgctttc aagtctgtct atgacgtcat gaataattgg
     5341 ggagctaatc acggagccat aacatatggc cacattggag cagacttgat taccttggct
     5401 tctatgttga gaattcctgt caatatgcat aatgtacctg aggaagatat ctttagacct
     5461 aaaaattggt ccttatttgg aacagaagat ctagaatcag cagactatcg tgcatgtcag
     5521 ttgttgggac cactacataa ataaaacttg tttatatagg aggtgaactt acgtccctcc
     5581 tatcctttta aaaagatttg ttaaacaatt cacaaataat tgaaaacgaa tacaaaaagt
     5641 aatataatga tgttaaatag atagcgcgga ggcgcaggag gaaaattata tggctatatt
     5701 ttatgttccg gcagtcaacc ttattggaaa aggtgttgta aatgaagtgg gtccttatat
     5761 caaggaactt ggctataaaa aggcactttt ggttacagat aagtacatcg aaggcagtga
     5821 tattttacct aaggttttaa aaccactgga tacagaagga atcgaatatg tcatctttag
     5881 cgatgtagag ccaaacccta cttgtaagaa tgtcacagat ggggtagctg ctttgcaaga
     5941 acatggctgt gattttatca tcagtcttgg cgggggttct ccacaggatg cagctagttg
     6001 tatttctatc atggctacaa atggtggaaa accacaggat tatgaagggc ttcataagtc
     6061 tgctaaaaaa ggccttcctg ttgtagctat caatactacg gcaggtacat cagcagaaat
     6121 taccattaac tatgtgatta ctgatgaaga acgcaaggtt aagatggtaa tggttgacaa
     6181 gaatagcctt gccttgattt ctgtcaatga ccctgaactc atgctttcca aacctcaagc
     6241 cttgactgct gctactggta tggatgctct gactcacgct gttgaagctt tggtaacacc
     6301 tggtgcttat gatgtaacca agaaactgtc tattggtgct attgagctta tcaaggaata
     6361 tcttcctcgt gctgtagcaa acggacatga tattgaagcg cgtgaaggta tggtcaatgc
     6421 catcttcctt ggtggtatga gctttaataa tgctggtctt ggctatgttc actcaatggc
     6481 tcaccaactc ggtgcagtat ataatttgcc acatggtgtt tgctgtgcca tgttgctacc
     6541 agttatagaa cgtgaaaatg ctaaacgtgt accagaagct ttccgcaatg ttgccaaagc
     6601 cttgggactt catgtagaag gtaaatcaga tcaagaatgt gccgattatg cgattgctga
     6661 gattgagaag ctttctgaga cagtaggtat tcctaagaaa ctgactgaac ttggtattga
     6721 agaaaaagat ttcgactttg aatacctttc taagaatgcc ttgattgatg cctgcgcacc
     6781 aggaaatcca tttatgccaa ccttagaaga aacgattgcc ttttataaag agttatttta
     6841 gcaaggatta gacgacaata ggacaattca cctctaatta ttgacatttc aggaaaaatc
     6901 gcagtaaaaa cggcacagcc ttctttaaat gtgctataat actagaaaaa tacttgtgga
     6961 ggttccatta tggcaatatt tttcatgatt tttctgattg tttgtgtgct cctattggtg
     7021 atagtcacac tgagtacagt ttatgtggtt cgtcagcagt cggtggcgat tattgaacgc
     7081 tttgggaaat accaaaaggt tgccaatagc ggtattcata ttcgcttgcc ttttgggatt
     7141 gactcgattg cagcacggat tcagttgcgc ttgttgcaaa gtgatattgt ggttgagact
     7201 aagaccaagg acaatgtgtt cgttatgatg aatgtagcga ctcagtaccg tgtcaacgag
     7261 cagagcgtga cagatgctta ctataaactc atacgtccag aatctcagat taaatcttat
     7321 atcgaagatg ctcttcgctc ttctgttcca aaattaacct tggatgaatt gtttgagaaa
     7381 aaagatgaga ttgcccttga agttcaacac caagtagcag aagaaatgac cacttacggc
     7441 tacattatcg tgaaaacctt gattaccaag gtcgaaccgg atgcagaagt taagcaatcc
     7501 atgaatgaaa tcaatgcggc gcaacgtaag cgggtcgcag cacaagaatt ggcggaagct
     7561 gacaagatta aaattgtcac tgcagctgaa gccgaagcag aaaaagaccg ccttcatggt
     7621 gtggggattg cccaacaacg taaggcgatt gtggatggat tggcagagtc tatcaccgaa
     7681 ctcaaggaag ccaatgttgg catgacagaa gaacaaatca tgtctatcct cttgaccaac
     7741 cagtatttgg ataccttgaa tacctttgcc tctaaaggaa atcaaaccat ctttttacca
     7801 aatacgccaa atggtgtgga tgatatccga acacaaatct tgtcagccct tcgcgctgag
     7861 aagaaataat agactaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgc
     7921 cgtatatctg caacctcaaa acagtgtttt gagctgactt cgtcagtctt atctacaacc
     7981 tcaaaacggt gttttgagca acctgtggct agtttcctag tttactattt gattttcatt
     8041 gagtacaaag agcttggcaa caggctcttt ttgtattgcg tttattaaga aaaacaaaaa
     8101 acatagatat acatttaact aaaaattatt gtatgttatc tcccttaatt aggaatgata
     8161 agggaataac tagaaagatt tgtgaataca aactatttct gatatacaaa atatacagta
     8221 ataatgaatg atgggagatg ggatgaaaga atttcaattt gagagaaagc agcgtttttc
     8281 tttgaggaaa tatgcaatag gagcttgttc ggtcttgcta ggaacgagtt tattttttgc
     8341 tggtatgggt gctcagcctg tacaggatac agaaacgagt tcagcactaa tttcaagtca
     8401 ttatttggat gagcaggatt tatctgaaaa gctgaaatct gagttgcaat ggtttgaatt
     8461 agaaaacaag cttttgaact tatgggagca ttaggttact atgaaggatt tgttccttat
     8521 gtttcaaatc aatacaaaaa ccaagctgaa gaagaagaca aaccgctatc tgataaatat
     8581 attttcgaaa aaatcttagg aaaaacatat gcagccttca aaaaagatca aattaatgag
     8641 cgtgtcgaga aattag
//
LOCUS       DAVMAF010000077         8609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMAF010000077 DAVMAF010000000
VERSION     DAVMAF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.078"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            8..1009
                     /locus_tag="VYO97_001744"
     CDS             8..1009
                     /locus_tag="VYO97_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1754505.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1611..1691
                     /locus_tag="VYO97_001745"
                     /pseudo
     CDS             1611..1691
                     /locus_tag="VYO97_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1996..2151
                     /locus_tag="VYO97_001746"
     CDS             1996..2151
                     /locus_tag="VYO97_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754506.1"
                     /translation="MEKLQFFLSQRACGCWKQAEKAVKLGSSETREENIILKGVPFIA
                     QPVTGDI"
     gene            2286..3647
                     /gene="trpE"
                     /locus_tag="VYO97_001747"
     CDS             2286..3647
                     /gene="trpE"
                     /locus_tag="VYO97_001747"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1754507.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYLYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGAMDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKSTLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3644..4210
                     /locus_tag="VYO97_001748"
     CDS             3644..4210
                     /locus_tag="VYO97_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1754508.1"
                     /translation="MILLIDNYDSFTHNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDKAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4221..5225
                     /gene="trpD"
                     /locus_tag="VYO97_001749"
     CDS             4221..5225
                     /gene="trpD"
                     /locus_tag="VYO97_001749"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1754509.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLAFK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5222..5989
                     /gene="trpC"
                     /locus_tag="VYO97_001750"
     CDS             5222..5989
                     /gene="trpC"
                     /locus_tag="VYO97_001750"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1754510.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            5976..6575
                     /locus_tag="VYO97_001751"
     CDS             5976..6575
                     /locus_tag="VYO97_001751"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1754511.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEVAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6553..7776
                     /gene="trpB"
                     /locus_tag="VYO97_001752"
     CDS             6553..7776
                     /gene="trpB"
                     /locus_tag="VYO97_001752"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1754512.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7769..8545
                     /locus_tag="VYO97_001753"
     CDS             7769..8545
                     /locus_tag="VYO97_001753"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1754513.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8517..>8609)
                     /locus_tag="VYO97_001754"
     CDS             complement(8517..>8609)
                     /locus_tag="VYO97_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1754514.1"
                     /translation="LLQKKKERLPFVPFLLLATCLIIFGKLLLV"
BASE COUNT         2546 a         1617 c         2076 g         2370 t
ORIGIN      
        1 gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc
       61 aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga
      121 aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc
      181 aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc
      241 ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt
      301 tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta attcaattat
      361 atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat
      421 cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac
      481 gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc
      541 tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg ggaggagaat cccatgttaa
      601 taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt
      661 aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt
      721 gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt
      781 gattcgtgct tgctatgaga aaggaattct tattccagat gatatttcta tcattggatt
      841 tgataatatt cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta
      901 tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt
      961 cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat
     1021 tcttgaaaat cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat
     1081 tcagtttcta ttgagttgga tccaatttct aacgagtggg acatgttcat gttcgatcta
     1141 aggaagacat agtcacattc gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa
     1201 agaaaaaaca aagtattaaa ataagttcaa caaatcagaa aagtaactat ttttgatctt
     1261 cagggagcct gtggtgattg tgaataggtg gttggaagta gtgaaagtgg gctgattttg
     1321 aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct tacagtgcag cttgttgtta
     1381 aacaaggcag agatgtaaag ggataatccc tttataattg aggtggcatc gcgttatcaa
     1441 cgccctcaca cggaagtata ttctgtgtgt gggctttttt ctatccgtcg tttggtttat
     1501 cttttattag ggccttaagt cgctttgatg aacttgagtt ctatctacag ctccttgcct
     1561 actactaaaa gcaaacaaaa aggctggact aatacgaaaa aagctagtaa tactcaatga
     1621 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaaacactgt tttgaggttg
     1681 tggatagaac tgacgaagtc agctcaaaat actgttttga ggttgtggat agaactgacg
     1741 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac
     1801 actgttttga ggttgcagat agaactgacg aagtcagtaa ccatacctac ggcaaggcga
     1861 cgttgacgcg gtttgaagag attttcgaag agtataaagg tgtaccgaga tgaacatctc
     1921 ttcatcaatg tctctggtcg tggaggcaag gacctagctg cgattgcaga ctacctagaa
     1981 gctaaaaaat aatagatgga aaaattacag ttctttctct cacagagagc ttgtggttgc
     2041 tggaaacaag cagagaaagc tgtaaagttg ggttcatctg aaacgagaga agaaaacatc
     2101 attctcaagg gagtgccctt tatcgcacag cctgttacag gagatatctg agacaggaat
     2161 gagagatagg agaaatccta taattgaggt ggcaccgcga atttcgtcct cacgcaagtt
     2221 attttgcgtg gggatttttc atatagtcac cacggactag aagtagaact gaaagggaaa
     2281 ttacaatgga acgaatcatt catggagatg tcttatcacc aatcttggct tatatgcgct
     2341 taaaggggca acacaaggtt atcttagaga gtattccgag agacaaggaa acagctcgtt
     2401 tttctatctt agcctataat cctgtttttg agattaagtt tgaaaatgga gttctttatc
     2461 aaaatggtca agtgattgat cgggatccct tggatttcct ttatgaagtg attcataaga
     2521 gtcagcacca ctcagagcta ccttttggtg gtggagccat tggttttgtg ggttacgata
     2581 tgatttcgct ttatgaagaa attggtcaaa tccctgagga tacaattggg acgccagaca
     2641 tgcatttctt tgtctatgag agctatatgg tctttgacca caagaaggag aagatacatg
     2701 tcatagagga tgcactttat agtgagcgca gtcaagaagc cttggaaaaa tccttgaacc
     2761 aagtgcttga ggaattacgc attcctgctc caaatgaatt tgaagacttg gatctatctc
     2821 cgttagactt caaaccccat atcgctcctc agaagtttga ggaaatggtg gaaaccgctc
     2881 gcgacttgat tcgtaatggc gatatgttcc aatgtgtact cagtcagcgc ttctcagcag
     2941 aagttactgg aaatccattt gacttctaca gaaatctccg cgtgaccaat ccatccaatt
     3001 acctctattt atatgacttt ggggattatc aaatcatcgg tgccagtcca gaaagtttgg
     3061 tttctgtcaa aaatggcatc gtgacaacca atccgattgc agggacgcga ccaagagggg
     3121 ctatggatga agaagacaag gccttggcga ctgacctcct ctctgatgag aaggaaacag
     3181 cagaacatcg aatgttggta gacttggggc gtaacgatat tggccgcatc tctgaaacgg
     3241 ccagtgtcca agtcactaag tatatggaag tggagctctt ccgttatgtt atgcatctga
     3301 ccagcgtggt caaggggcgt ttgcttccag aactcactgc tatggatgcc ttgaaatcaa
     3361 cgcttccagc tggaaccgtt tctggagcac caaaaattcg agctatgaga cgcatctatg
     3421 aactggaaac ggaaaaacgg ggcgtatatg caggagcaat cggctacttg tctgcgacgg
     3481 gtgatatgga tttggccatt gccatccgaa ccatgattct caaaaatcaa agagcctatg
     3541 tgcaggctgg ggcagggatt gtctacgact ctatcgccca aaacgaatac caagaaacca
     3601 ttaacaaagc aaaatctatg actagaattg gagaactaag accatgattt tattgattga
     3661 caactatgat tcttttaccc ataacttggc ccaatacatt gggaattttg cagaagttca
     3721 ggttctgaga aatgatgatt ccaagctgta tgaagaagct gaaaaagcag atggtctggt
     3781 cttttctcct ggtcctggtt ggccagttga tgctggaaag atggaagaca tgattcgtga
     3841 ttttgcaggc aagaagccga ttcttgggat ttgtttgggc caccaagcca ttgcagaagt
     3901 ctttggtggt aagctaggtt tggctccaaa agtcatgcat gggaaacaga gcaatatcaa
     3961 ctttgaagcg ccatctgttt tgtatcaagg tattgaggat ggccgtgcgg tcatgcgtta
     4021 tcacagtatt ttgattgagg aaatgccaga agactttgaa gtgacagctc gttcgactga
     4081 tgacaaagcc atcatgggga ttcaacataa aaacctaccg atttatggct tccagtacca
     4141 tccagagagc attggaacgc cagacggctt gtcttctatt cggaatttta tcgagaaggt
     4201 tgtaaagtga ggaaactagg atgaaagaga ttattgaaaa actagcaaaa tttgaaaatt
     4261 tatcaggtgt ggaaatgacg gatgtcattg agcgtatcgt aactgggcgt gtaactgaag
     4321 cgcagattgc ttctctcctc ttagctttta agatgaaggg ggaaacacct gaagaacgca
     4381 cagccattgc ccaagtcatg agaggacatg cccagcatat tccaactgag attcatgatg
     4441 ctatggacaa ctgtggtaca ggtggggaca agtctttcag ttttaatatt tccacaactg
     4501 cagcctttgt cttggctggt ggcggtattc acatggccaa gcatggtaac cgctcgattt
     4561 cttctaaatc tggttccgca gatgtcctcg aagccttggg aatcaatctt gacctcaaac
     4621 cagctgaact aggtaaggtc tttgataaaa ctggaatcgt ctttctcttc gctaaaaata
     4681 tgcacccagc tatgaaatac atcatgccag ctcgtttgga attgggaatt ccaacgatta
     4741 tgaatttgac tggtccactg attcatccaa tggctttaga aacacagctt cttggaatta
     4801 gtcgtccaga actcctagaa agtacagctc aggttttgaa aaatatgggt cgcaaacgtg
     4861 ccatcgtggt tgctggacca gaagggttgg atgaagctgg cttgaacgga acaaccaaga
     4921 ttgcacttct tgaaaatggc gaaatcagct tgtcaagctt tactccagag gatttgggaa
     4981 tggaaggcta tgctatggaa gatattcgtg gtgggaatgc tcaggaaaat gcagaaattt
     5041 tgcttagcgt tctgaaaaac gaagcaagtc cattcttgga aacgacagtc ttgaatgctg
     5101 gtcttggttt ctatgctaat ggtaagattg atagcatcaa ggaaggagtt gccttggccc
     5161 gtcaagtgat tgctagaggc aaggcccttg aaaaactcag actgttacag gagtaccaaa
     5221 aatgagtcag gaatttttag cacgaatctt agagcagaag gcgcgtgagg tggagcagat
     5281 gaagctggag caaatccagc ctctgcgcca gacctatcgc ttggcagaat ttttgaagaa
     5341 tcatcaggac cgcttgcagg taatcgctga ggtcaagaaa gctagcccta gtttgggaga
     5401 tatcaatctc gatgtggata ttgtgcaaca ggcccagact tatgaagaaa acggagcagt
     5461 gatgatttcg gtgttgacag atgaggtttt ctttaaaggg catttggatt atctacggga
     5521 aatttccagt caggtagaga ttccgacgct caacaaagac tttatcatcg atgaaaagca
     5581 aatcatccgc gctcgcaatg caggtgcgac agttatcttg ctcattgtgg cagccttggc
     5641 agaagaacgt cttaaggaac tttacgacta cgcgacagtg cttggtctgg aagtcttggt
     5701 ggaaactcac aatctagctg aactagaagt ggctcacaga cttggtgccc agattatcgg
     5761 ggtcaataac cgcaacttga ccacctttga ggtcgacttg cagaccagtg tagacttggc
     5821 taaacatttc aaagatgatt gcttgtacat ttctgaatct gctattttca cagggcagga
     5881 tgcggaacga ctagccccat actttaacgg aattttagta gggacagctc tcatgcaggc
     5941 agaggatgtg gcccagagaa tcaaggagtt gcagattgac aaaggttaag atttgtggac
     6001 tatcgaccaa agaagcggtg gaaacagccg tttcagcagg agccgactac atcggttttg
     6061 tcttcgcacc tagtaaaaga caggtaactt tagaagaggt agctgagttg gcaaagctta
     6121 ttcctgcaga tgtgaaaaag gttggagtat ttgtttcacc aagtcgggta gaactgctgg
     6181 aagcgattga caaagttggc ttggacttgg ttcaagttca cggtcaggtg gcagatgatt
     6241 tatttgagaa tttgccttgt gccagtattc aggctgtgca ggtagatgga aatgggcatg
     6301 tccccaattc tcaggcagat tatctactct ttgatgcccc tgtggcagga agtggccagc
     6361 cctttgattg gggtcaactg gatacgactg gactagcaca gcccttcttt atcgcaggtg
     6421 gccttaatga agataatgta gtaaaagcaa ttcaacattt tactccctat gcagtagatg
     6481 tatcgagcgg agtggagaca gatggacaaa aagatcatga aaagattaga agatttatag
     6541 agagggtaaa gcatggcata tcaggaacca aataaagatg gattttacgg aaaattcggc
     6601 ggacgttttg tcccagaaac attgatgaca gcagttttgg agttggagaa ggcctaccgt
     6661 gaaagtcagg cagacccaag tttccaagag gaattaaacc agcttttgcg ccagtacgta
     6721 ggacgtgaaa ctcctcttta ttacgcaaaa aacttgaccc agcatatcgg cggagccaag
     6781 atttatctca aacgggaaga ccttaaccat acaggagccc acaagattaa caatgcctta
     6841 ggacaagttt ggcttgccaa acgcatgggt aaaaagaaaa ttatcgcaga aacgggtgct
     6901 ggtcagcacg gtgtggcaac tgcaactgct gcggccctct ttaacatgga atgtaccatc
     6961 tacatgggtg aggaagatgt caaacgccaa gccctcaatg tcttccgcat ggagcttttg
     7021 ggagccaagg tcgaggctgt aacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca
     7081 gcccttcgtt catgggtggc taatatcgac gatacccact atatccttgg ttctgccttg
     7141 gggcctcatc catttccaga aatcgttcgt gacttccaaa gtgtcatcgg tcgagaggct
     7201 aaacaacagt accgtgactt gacaggtcga gatctgccag atgccctagt agcctgtgtt
     7261 ggtggtgggt cgaatgctat cgggctcttc catccctttg tagaagatga gtcagtagct
     7321 atgtatggaa ctgaagcagc agggcttggt gtggatacag agcaccacgc tgctaccttg
     7381 accaagggtc gtccgggtgt tcttcacggt tctctcatgg atgtgctcca agatgcccat
     7441 ggtcaaattc ttgaagcctt ctctatctca gcaggtttgg actatcctgg tatcggtcca
     7501 gagcattctc actaccacga tattaaacgt gccagctatg tccctgtgac agacgaagaa
     7561 gccttggaag gatttcaact cttgtctcgt gtggaaggga ttatcccagc tttggaatct
     7621 agccatgcta tcgcttttgc ggtgaaattg gccaaagaac ttggaccaga aaagtctatg
     7681 attgtctgcc tatcaggtcg tggggacaag gatgtggttc aagtcaaaga ccgcttggaa
     7741 gcagatgcag caaagaaggg agaagctcat gcctaagaca ctaacagaaa aattgaatgc
     7801 tataaaagta actggaaaag gaattttcgt tccttatatc atggctggtg accacgaaaa
     7861 aggtttggac ggtcttgctg aaacaattca ctttttagaa gatttgggtg tctctgctat
     7921 tgaagtgggg attccctttt cagaccctgt tgcagatgga cctgttatcg aagaagctgg
     7981 cttgcgcagt ttagcccacg ggacctctac ccaggcttta gttgaaacct tgaaaaccat
     8041 tgaaacagag attccactgg tcatcatgac ctacttcaac cccctctttc agtacggtgt
     8101 ggagaacttt gtcaaagatc tggctgatac agctgtgaag ggcttgatta tcccagacct
     8161 gcctcatgaa catgccaact ttgtagaacc atttttggca gatacagata tcgccttgat
     8221 tcctttagta agcttgacca caggaattga gcgccagaaa gagttgattg aaggggcgga
     8281 gggattcgtc tatgccgttg ccatcaatgg ggtgacaggg aaatcaggca attaccgtgc
     8341 agatttggac aagcacttgg cgcaacttca tcaagtggcc gacatcccag tcttgacagg
     8401 ttttggtgta tctagtcaag ccgatctgga acgcttcaat gcggtgtcag atggtgttat
     8461 cgtcggttca aaaatagtaa aagctctcca ccaaggagag ccgattcagg attttatcag
     8521 acaagcagta gcttaccaaa aataatcaaa caagtagcaa gtaagaggaa aggcacgaaa
     8581 ggaagtcttt cctttttctt ttgcaggag
//
LOCUS       DAVMAF010000078         8521 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMAF010000078 DAVMAF010000000
VERSION     DAVMAF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.079"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(232..303)
                     /locus_tag="VYO97_001755"
     tRNA            complement(232..303)
                     /locus_tag="VYO97_001755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Arg,seq:cct)
     gene            complement(346..693)
                     /gene="rplS"
                     /locus_tag="VYO97_001756"
     CDS             complement(346..693)
                     /gene="rplS"
                     /locus_tag="VYO97_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1754515.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(812..1141)
                     /gene="crcB"
                     /locus_tag="VYO97_001757"
     CDS             complement(812..1141)
                     /gene="crcB"
                     /locus_tag="VYO97_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1754516.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1135..1509)
                     /gene="crcB"
                     /locus_tag="VYO97_001758"
     CDS             complement(1135..1509)
                     /gene="crcB"
                     /locus_tag="VYO97_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1754517.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1506..1772)
                     /locus_tag="VYO97_001759"
     CDS             complement(1506..1772)
                     /locus_tag="VYO97_001759"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1754518.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1888..2331)
                     /locus_tag="VYO97_001760"
     CDS             complement(1888..2331)
                     /locus_tag="VYO97_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1754519.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2435..3370
                     /locus_tag="VYO97_001761"
     CDS             2435..3370
                     /locus_tag="VYO97_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1754520.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3424..3708
                     /locus_tag="VYO97_001762"
     CDS             3424..3708
                     /locus_tag="VYO97_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1754521.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3765..4343)
                     /locus_tag="VYO97_001763"
     CDS             complement(3765..4343)
                     /locus_tag="VYO97_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV1754522.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(4318..5331)
                     /gene="efeB"
                     /locus_tag="VYO97_001764"
                     /pseudo
     CDS             complement(4318..5331)
                     /gene="efeB"
                     /locus_tag="VYO97_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
     gene            complement(5334..5793)
                     /locus_tag="VYO97_001765"
                     /pseudo
     CDS             complement(5334..5793)
                     /locus_tag="VYO97_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            6517..7446
                     /locus_tag="VYO97_001766"
     CDS             6517..7446
                     /locus_tag="VYO97_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1754523.1"
                     /translation="MNKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            7460..8383
                     /locus_tag="VYO97_001767"
     CDS             7460..8383
                     /locus_tag="VYO97_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1754524.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
BASE COUNT         2540 a         1825 c         1561 g         2595 t
ORIGIN      
        1 ttaaagaaat atgaatgtta tagtccttta aataatttag aacttgacga ttttgaataa
       61 attgactata caaacattta tatactcgat atagtctcaa ttttatctga taattgccca
      121 aatttttcaa taataaaacg cataatatta tggagacaca caaaatcacc tatattatgc
      181 gttcttttaa tatcaaagac tttttgacaa acttctttga tgtctaatta ctgccccctg
      241 caggaatcga acctgcaact actccttagg agggagttgt tatatccatt gaactaaggg
      301 agctagataa aaactctgct aaatgagcag agttttttag tcgaattaac gacggatttc
      361 tttgatacga gctgctttac cttgaagagc acgcaagtag tacaatttcg cacgacgtac
      421 tttaccgtaa cgaacaactt cgattttttc aacacgtgga gtgtggattg ggaagatacg
      481 ctcaacacct acaccgttag agattttacg aactgtgtag ttttctgaga ttccagcacc
      541 tttacgtgcg ataacaacac cttcaaaaat ctggatacgt tcacggttac cttcgacaac
      601 tttcgcatgt acacgaacag tgtcaccagg acggaatgat gggatatctg tacgaagttg
      661 accttcagtc aagctttgga ttaatggatt cattttattc tcctatcttt gtcaatcttg
      721 aggaatcttc ctcagcggat aaactgtatt tttgtgcgtc cattacacac aagatatagt
      781 ttaccaaatt tctaaaaaaa agtaaagaaa tttatagcaa aattcctaaa aaaatcgcaa
      841 ccaaaccacc tatgtaagtc aaagtcaggt aagaataaaa gaccttctta tcacttagca
      901 gtctttgaag ttcgtcattc aaggtcgaaa aagttgttaa accgccacaa aatcctgttg
      961 ctagaatagc atatacttcc ttagactcta catgattgta gaatactcca attaaaaaac
     1021 aacctaaaag attagctatg agcgttccaa gtggcaattt agaagcttga ttgtagcggg
     1081 aaaagaaata ccgtacgagt gctccgaaac cacaagcaat tgcaagatag acgattacca
     1141 tttcttcctc cctagaaaat aagctaagag taggcctcca ccgatgctca aaagcaaata
     1201 caggactaaa ctaagataac gccctgtatc aagcagcttc acagtatcaa gcattaggct
     1261 ggaaaaagtt gttaaacctc cgcaaaaacc cgtccccagt gctaaaatca agcccttact
     1321 agtcccctta tagaccagat agcccttgac aagaaaaatc aagcagaaaa ttcccagata
     1381 gttgacaaag agagtgcccc aaggaaagtc tggactggct ggtaaccagg tggaaactag
     1441 atatcggaca agtccgccca acatagcagc tagaaaaatt cctagcggat aaaattgttc
     1501 ttttttcatt tgatattttg atcctgataa tcacgcgaac gtttgagtat gtccgaaaaa
     1561 gtcgcgacaa tagtctcctg atagcgcttg tcctctacac gactcctaac cttttcaaaa
     1621 ataacttctt ctctcttagt atctaaaatc ggtttacctg aagctttctt ataagcaacc
     1681 accccctcaa ctaaatgcat tcgttcttct aacagtttga caatttggtc gtcgatttga
     1741 tcgatttctt gacgaataat atctaaatcc atacagtctc ctcctttatt tgaattattg
     1801 tatcaaaaag ccatcaatta ggctagtaaa atcccaactc tcgataagaa aaatgcactg
     1861 attgattaca tcagcgcact tttatgttta tcctacttta gcagccaatt cttctgcgaa
     1921 ttgttccaag cgttcaatat cttcttcctc ggcagaaaga tcaactttaa cacactctga
     1981 acctttttct gctcctgttg acacaaagac acggtcaaag tcatcaacag ccttacagaa
     2041 ttcatcgtag aaggtatctc ctgagccgac cactccgtag attttgccat tcaagttgag
     2101 atctgctagg tcttcgtaga agtccatcat ctcatctggc aattcaccat caccataagt
     2161 ataggtcgca acgatagcga tgtctgcttc caagaagtct gaagcgtcaa cagttgtaca
     2221 ttcatcaaca tcaacatcca agcccaagtc acgtaatttg tctgctacaa tatctgcaat
     2281 ttcttcggta ttaccggtca tactggcaaa tacaattttt gctaatgcca tatcgtcctc
     2341 ctcaatttat ctttctccat tatatcacat ctttttcatt ttaaaaataa aaattcttgt
     2401 gctacaatga aatgagaaag aattgaggtt atttatggag atttgccaac aaattttaga
     2461 aaaaattaaa gaatacgaca ccattatcat tcatcgtcat atgaaaccag accctgatgc
     2521 cttgggaagt caggtgggat tgaaagcctt gctggaacat catttcccag aaaaaaccat
     2581 caaagccgtc ggttttgatg aaccaactct tacttggatg gctgagatgg atcttgttga
     2641 agatagagcc taccaaggcg cacttgtcat cgtctgtgat acagctaata ctgctcgtat
     2701 cgatgataag cgctatagtc aaggtgattt tctcattaag attgaccacc atccaaatga
     2761 tgatgtatac ggtgacctgt cttgggtcga tactagttca agtagcgcta gcgagatgat
     2821 taccctattt gcccaaacaa cccaactagc cttggcagat cgcgatgctg agttgctctt
     2881 tgcaggaatt gtcggtgata caggtcgctt cctctaccct tctaccactg cacggactct
     2941 tcgcctggct gcttatttga gagaacataa ctttgacttt gcggctctca ctcgcaaaat
     3001 ggacactatg agctacaaaa ttgctaaact gcaaggctac atctacgacc atctggaagt
     3061 ggatgaaaat ggtgctgctc gcgttatcct gagtcaagaa gtcttgaaac aatacaatgt
     3121 aaccgatgct gaaactgcag ccattgtcgg tgcacctgga cgcattgaca gagtgagtct
     3181 ctggggaatt tttgtcgaac aggctgatgg ccactaccgc gttcgcttac gcagtaaaat
     3241 ccatcctatc aatgaaatcg ccaaggagca tgatggtgga ggccaccctc tagcaagtgg
     3301 tgctaattcc tatagcctag aagaaaacga aatcatctac caaaagttaa aaaacttgct
     3361 taaaaactga taaaatactt gccaaacttt tcagaatctg atagactagt atggtaacaa
     3421 tctatggctc gcaaagagac catggcagaa aggaaatatt gcaaaatgaa aaaagatatc
     3481 catccagaat atcgcccagt tgtcttcatg gatacaacta ctggttacca attccttagc
     3541 ggttcaacaa aacgctctaa cgaaacagtt gagttcgaag gcgaaactta cccattgatc
     3601 cgtgtggaaa tttcatcaga ctcacaccca ttctacactg gacgtcaaaa gttcactcaa
     3661 gcagatggac gcgtggatcg tttcaacaaa aaatacggtc tcaaataatg ataagagaac
     3721 agtttcggct gttctttttt gtttcttgaa atcaactgct gttttcatgt tccagactca
     3781 tctgtaggtt cggtttccat gctactaggc aggaaggaaa tagctgtttc aacacgtcca
     3841 taatgagctg tactattgtc acgaaccaca ctttcattga tggtccaagt ggaattcatt
     3901 ttcttaaaag cttctcggac tttttccaaa tctttggagg caatggcctg ttctaaggtt
     3961 tcaaaacgag gacttagacg gtttaccaat ttttcctttt ccgcatctag gtcaacagga
     4021 ttttgttctt tttcaaaagc taatagagct gaggaaattt cggtgagttt ggcttcagta
     4081 atatctccag atagagctaa cttttcctta acaatcttac caacctcaga atccttattt
     4141 tctactcttt cgaagtctga tgccatcttc gtaacgagtt tctgggtctc agtctgacca
     4201 ccatttttaa ccgttttgga tgcatctgtg attttcacaa agaaaaaact caagctttct
     4261 tgggcacctg ctggggaaag aattaaaaat gacaaagcta aaactagtaa ccagctttta
     4321 gccttccaat aatttctgac caatgtagcc tcctttctcc acaccaccaa agcaagtaaa
     4381 aagtccacta cctatgtgag tgatgtattc attcatctta tcagtagcac ctaggttagt
     4441 ttgaacctta acaaagttat caggatcctt ctggaaagaa atgaagagca atcccgtatc
     4501 aaactgacca gtcttttcat caatgccatc tgagtaagaa taagaacgac gtaaaagtgg
     4561 cttatccact tctttggcca aacaaacatg cgaatcatca ggtaagagac tcaaatccac
     4621 ttcatcaaac tcatttttct taccaaaggg ggcaccgctt tccttgtaac gaccaaaggt
     4681 attttcttgt tcttcgagac tagtgcggtc ccaggtctct aaaaacatct gaatccgacg
     4741 aactgccata taagaaccat tttccatcca gtccttacta tcagcccaga taacgcggtc
     4801 aaagtcttgc tccttggttg gatttgctgt tccatcctta aaaccaaaga ggttccacgg
     4861 agtctccatt cgatccccga tagctgcaaa tccagactga ctccaacgaa ggggcaccgc
     4921 atttctcccc ttacggatga gattgcgaat agcgtgaaag gcaatttgct catcatctgc
     4981 acaagcatga atcacaatat ctccaccagt atatttttca cgtaattgct ccttaggaaa
     5041 tagaggtaaa tccctgaaca gtcgagggcg cttgttttcc aagttcatcc ttttcaagaa
     5101 acttgcagat acaccaaaag tcagcgtcaa acgatgagga ttgagcccaa ctgtctcacc
     5161 agtatcgctc ggaggaaaga gagcattctg tccatctttt ttaaccaatt ccccctcgac
     5221 caacttggca ctataatccg tccaatcctt gaatagctgg ataattttat ctctatccgt
     5281 cgtatgcagg tccaagacaa caaaatagat attcttttgc atgggcgtac tatttggtac
     5341 atcaagcgaa ccttatgaaa acgtctacta tatttaaaac gctttcttgt cttcttgaat
     5401 tgagatttta tttttagacg cgacaatggt ttgagcgtct gctttcttag gatgttctgg
     5461 acgtggcata atatttcgcc aaccatggcg cttcaacagt tgatagaagg catcacgtgt
     5521 gtaggaacga cctaactcct ttttataagc ctgaaataag gcatcaattg taacaaattc
     5581 tcctgcctct gcagccttca aatggcgggc aagaaagact ttcttttcct caactgtcat
     5641 atatgcatgg ttacgaccac tacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc
     5701 atattttttt acattttgct aaatcgttgt ttgattacag tctaaaagct ctataatctc
     5761 tttataagat ttgcccatca gacgaaatat aataaattga aactagaata gtacacctct
     5821 acttctaaaa cattgttaga aatcgatttg actgtcctaa tcgatttgtc ctgttcttgt
     5881 ttcattttac tatagaacga tttgaaggcg tttatgatat ttagctgtac gagagtcttt
     5941 taaaagtgtt ttgatagttt ggatttcttc tttagttgat ttcatatcac tgttatataa
     6001 tgctttttga ttttagtcta gtattagtaa gcagactcaa accttgaaac agcagataag
     6061 gatgtccttg aaaactctca agaagaactt caaaaagcag ttttgttctt ggcagatgaa
     6121 aaaggctctg agcatactga agcagagttg attgataatc ttaaagaggt tattgctaag
     6181 ttgaaagcaa acgcctaata acatattaaa aatctccaga ttaggaacta tcgctgagtt
     6241 ccctagtctg gagatttttc agtatacccc gttgttggat gcttataaca gagtgacgcc
     6301 atcactgggc taatagagat aaaataaaca attgatttag gacatttgtt tgatagtggt
     6361 gataaagttt taaatctgct acataatttg tggagttttt tctataatta ggagtttaac
     6421 ctaacctgca aataggagta tactaataat gtaatcgtta tcaaaagtct aaaaaagaat
     6481 ttttagatgg atatcaaaat aaaaagggag gaaattatga ataagttttc aaaaaccttg
     6541 agagacaact ggatctttct cttgatggtt ttgccagggg cactctggtt gattctattc
     6601 ttttacattc cagtatttgg gaacgtggtt gccttcaaag actaccacat gaccagtaat
     6661 ggtttcatag atagtatcat aaatagtaaa tgggtcggac tcgataattt cagattctta
     6721 tttagttcaa gagacgcctt tattatcaca cgaaatactg tcctctacaa tcttggcttt
     6781 atctttctag gtttagttgt atctgtaggg attgccatta tcctcagcga gctccgttct
     6841 aagagaatgg tgaagatttt ccaaacttct atgttgttcc cttacttctt gtcttgggtt
     6901 atcatcagtt tctttacaga tgccttccta aatattgata aaggggtgtt caatcatcta
     6961 ttggaaagtc ttggtctcaa agaagtcaat ttctacgctg acctgggcat ctggccctat
     7021 ctcctacttt tcctaggtat ttggaaaggt tttggatata gcagtgtcat gtactatgcg
     7081 acgatcatgg gaattgatcc aacctactac gaagcagcga cagtggacgg agctagcaag
     7141 tggcaacgta ttcgcaacgt aaccattcct cagttgactc cgcttgtaac tgtattgacc
     7201 atccttgcag tcggaaatat cttccgcgca gacttcggtc tcttctatca aatcccacac
     7261 aatgctggtc agctttacaa tgtaaccaac gttttggacg tatatgtttt taatggtttg
     7321 actcagacag cagatatcgg tatggctgca gcagccggtc tttaccaatc cgttgttggt
     7381 ttgattctgg ttatcctatc aaacttgctt gcaagacgag tcgatccaaa ctcagctttg
     7441 ttctagaaag gaggagaata tggcaaaaaa gaaaattaaa aaagaaaaaa ttgataatgt
     7501 cggcattcac tccttcagta agaaagcaga tatcttcttc agtatcattt cgggtttgat
     7561 tgccctctct tgtatcctac cttttgtatt cgttatcatt atttcagtga cagacgagaa
     7621 aagcctcctc caatacggct atagcttctt cccatcccaa tttggcttag acggttttga
     7681 gtttttggca cagtttaagg ataaaatcct acaagccctc ttcatctctg tctttgtaac
     7741 cgtagttggg acattgacaa atgtctttat cacaacaacc tatgcctacg ctatttcacg
     7801 gacaaccttt aagtatcgca gattctttac catcttcgtt ctgctcagta tgttgttcaa
     7861 cgctggtttg gtaccaggtt atatcatggt gactcgtgtg cttcagcttg gggatactgt
     7921 ttgggccttg attgttccaa tgcttctctc accatttaac atcatcttga tgcgttcctt
     7981 cttcaagaag accattccag aagccattct agaatcggct cgtatcgatg gtgccagtga
     8041 agcccggatt ttcttccaaa tctgtttgcc attgtcacta ccaggtatcg caaccatcac
     8101 gctcttaaca gctcttggtt tctggaatga ctggttcaac gcccttcttt acatcaagag
     8161 tgacaacttg tatccattgc aatatttgct catgcaaatc caacaaaata tggactacat
     8221 tgccaaagcg gtcggcttga ctggtcaact gggagttgct ctaccgaaag aaacaggtcg
     8281 tatggccatg gttgtggttg caacccttcc aatcgcgatt ttgtatccat ttttccaacg
     8341 ctactttgta aaaggtttga ctatcggtgg tgtgaaagaa tagtgcttat tgagaaataa
     8401 catttctcag ttcccaactt cccttgtgtg aagttaatac tcttcgaaaa tcaaattcaa
     8461 accgtgtcaa cgtcgccttg ccgtagatat atgtaactga cttcgtcagt cttatctaca
     8521 a
//
LOCUS       DAVMAF010000079         8437 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMAF010000079 DAVMAF010000000
VERSION     DAVMAF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.080"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            53..640
                     /locus_tag="VYO97_001768"
     CDS             53..640
                     /locus_tag="VYO97_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1754525.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(692..1012)
                     /locus_tag="VYO97_001769"
     CDS             complement(692..1012)
                     /locus_tag="VYO97_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1754526.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            1438..1725
                     /locus_tag="VYO97_001770"
     CDS             1438..1725
                     /locus_tag="VYO97_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1754527.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            1777..3885
                     /locus_tag="VYO97_001771"
     CDS             1777..3885
                     /locus_tag="VYO97_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1754528.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTIPSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLSDVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSLFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHALLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            3882..4523
                     /locus_tag="VYO97_001772"
     CDS             3882..4523
                     /locus_tag="VYO97_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754529.1"
                     /translation="MIELDNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSTKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            4741..5538
                     /locus_tag="VYO97_001773"
     CDS             4741..5538
                     /locus_tag="VYO97_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1754530.1"
                     /translation="MKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALNPD
                     FAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLAI
                     SGVSKTDERSKVFDFSIPYYTAKNKLIVKESDLTTYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNFEKE
                     QDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            5504..5974
                     /locus_tag="VYO97_001774"
     CDS             5504..5974
                     /locus_tag="VYO97_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1754531.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQIEYQIAVAKKANEAKK"
     gene            complement(6264..7127)
                     /locus_tag="VYO97_001775"
     CDS             complement(6264..7127)
                     /locus_tag="VYO97_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1754532.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGARELSDHTEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            7593..7931
                     /locus_tag="VYO97_001776"
     CDS             7593..7931
                     /locus_tag="VYO97_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1754533.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            7909..8280
                     /locus_tag="VYO97_001777"
     CDS             7909..8280
                     /locus_tag="VYO97_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV1754534.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
BASE COUNT         2621 a         1346 c         1824 g         2646 t
ORIGIN      
        1 gtaatattta aagggtatga tagaactata gaaagaagga gaattttcga atatgaaatc
       61 aataactaaa aagattaaag caactcttgc aggagtagct gccttgtttg cagtatttgc
      121 tccatcattt gtatctgctc aagaatcatc aacttacact gttaaagaag gtgatacact
      181 ttcagaaatc gctgaaactc acaacacaac agttgaaaaa ttggcagaaa acaaccacat
      241 tgataacatt catttgattt atgttggtca agagttggtt atcgatggcc ctgtagcgcc
      301 tgttgcaaca ccagcgccag ctacttatgc ggcaccagcc gctcaagatg aaactgtttc
      361 agctccagtg gcagaaactc cagtagtaag tgaaacagtt gtttcaactg taagcggatc
      421 tgaagcagaa gccaaagaat ggatcgctca aaaagaatca ggtggtagct atacagctac
      481 aaatggacgt tatatcggac gttaccaatt aacagattca tacctgaacg gtgactactc
      541 agctgaaaac caagaacgtg tagcagatgc ctacgttgca ggacgttacg gttcatggac
      601 agctgctaaa aacttctggc ttaacaatgg ctggtattaa gaactaacga acaaataata
      661 tgtaaagagt cgaggaaatc cctcggcttt tttatctttc cttcgcttgg taatagatct
      721 ctacttcttt tttgagatga gacagcatag aagtttcctc ggttagctct aggagtgaga
      781 aacctttttg gataagtttc gcatcatccc tacaaatccc aatagggaca tagcagatag
      841 ccagcctatc gtagcttttc ttattcttgg cgtcctccag taaagtgtag cagatttgct
      901 tacacatgtt tttatcttga ttgtatagat agagtgtgga aaggttgagc aggattgcta
      961 ttcgcaagtc atataaatgt tgatagtttt tatagacttc taagcgttgc aagattttct
     1021 cggattattg acaaataaac ggacattctg atagacgatt gccctagcgg cttagtgtaa
     1081 actttacaaa tttggcttgt aatttttttt ataaaattca cttcagttac ttgacagtta
     1141 ttatattttc acttattata tagataaaag gatgggatag gagtttaaaa atttgaaagc
     1201 gctatccata ttgtttattg gactgattct tgagtaatag ttagagtgtg cttaatacta
     1261 ctctggttgt tcaaggacct gatacagaag cgcaccattc tcccggttaa gagtaagttc
     1321 gttgttaaaa aatgaaatga gatagaagga aggtgagtcc aatggactac gatacttgtt
     1381 gataaattac tatagctgtt tttacggaac agctctcgat aataggagaa ataaatcatg
     1441 agaaaattag ttaaaattgg tgttgcatct ttaatggtat gtggtatact tgctactaca
     1501 aatgctttag cagtatgggt tgagggtggt caatggaact atggagtagg ttggacagga
     1561 acttttggat attctgatta cttacattct actcgatatc atacagcaac tgttagacat
     1621 gggggtagaa cctctaagga ttatgcaaaa cctgaggcat gggctagagc ttccctcacc
     1681 aagattccgc caacaggaat ggaatatttc tatggatttg agtaaaattc ttccctctcc
     1741 tatttggaga gggaaaactg tagtggaggc acttttatga aaaaaatcag taatttctgt
     1801 atgttactcc tgcttctgtg taccactttt tttgttttta atgtaaacta tacacgagaa
     1861 gtggttcgga ttcaagaaat gggaaagact gtagattctt tggatttgta tttgaaagat
     1921 attaacgaac ctgcagcgtc tgttcttcga ttttttgagg atgtatcaaa ggagtataaa
     1981 gtctccatca tcaaaacaga cagtggtgat gaggtggtca agtctggtgt ttttgataaa
     2041 gataccttcc cctaccaaga gtttgggatt tcttctcttg attttaccac agatggtgaa
     2101 ggagtctata gtaataaaga aatttccaat aaacttggta cgattccgac ctttctaaaa
     2161 gccaaaccta ttcagcttat gacttttcaa acctatatca aggatacatc tcgtagttta
     2221 aatggtcgct atacgatacc ttctacacaa gagatggaca aggataggat tgtacagaaa
     2281 tggagcgatt ttttcaagat agaccaggct accttgctag agccgaccta caaaagtgca
     2341 gtggaagtca taaatcgaga tttgctttta tctgccattg tttttgtctt ggctattttg
     2401 cttcttgtgt tagtgacagt gtatcaaccg atgatggaga tgaaacgagt tggggtacaa
     2461 aaattacttg gttttcaaga tagggctgtt ttatctgatg ttgtaaaagg caacctttac
     2521 ctcctcctag gtggggctct tgtgatcaat ctaggcgtgt ttttcttgct tgattataag
     2581 ccaaaagatt tgtttcctat gctgtggttg tctctttttt tgctgttgca gctttatctc
     2641 tttatcagtt ggttgactta cctcttaatc caaaaaatga caatcagctc tctgctgaaa
     2701 ggtttttcat ctttcaaatt tggtctactc tttaattatt tgatgaaaat tggaactacc
     2761 attttactga cagtcttgct agttggggta ggaaaaagtt tggagcaaga aaacaaagag
     2821 ttggattacc agaaacagtg gatcagccaa ggaaattatc tgaccttaga aactttccaa
     2881 ctcaatgaca acttgtggca agagcagttg gcaggctcag ggaaggcatt ggattatttc
     2941 tatcgatttt atcaggattt ggtagaaaaa acgcaggcgg gctatgtgca gagtagcagt
     3001 cttcctgtaa aaaattttgt ccaatcagaa cagattcagc aatatcagtt aacagatacg
     3061 gtggatgttt actatgccaa tcgcaatttt ctaaagagca agggattcaa gctaccagat
     3121 accggtatta aaaaagttat tttgatgcca gcaagtacga aaggtgaaga agataaaaat
     3181 cagctcttgg ggaagttaat tgcctttcat tcgatgaagt atgaagagca gcaaaaacga
     3241 acgatagagg agatggatgt cgagattgcc tattatgaag gagattggtc atttttccca
     3301 tatagtgata agcgaaagga aaatctctcc aatccaatta ttagcttggt caatgattct
     3361 gatatgatgt gggatgagaa agcctccctg tcaacaactg gcttaaataa tccgattaaa
     3421 attgaaaata cggttcaaca tcaaaaagag attacagagt tagttgagaa attgtcagat
     3481 ggaaattatt taaaattttc atctattcaa gccattcaac aagagaaagt ggattcttat
     3541 cgagatgctg ttcggaattt taacctactc tttgctttgt ttggtctcct tagcatgatg
     3601 atttcctact tcttactagt aataactttc ttattgaagc gcagggatat tattaccaag
     3661 aagtttatgg ggtggaaact ggtcgatcgc taccgccctc tcctcgttct gctcttgctg
     3721 ggctatagtc tgcctcttct agtcttgatt ttctttgccc atgcgctctt gccactccta
     3781 ctttttgcag gttttacatg tttggatata ctatttgtgc taggcttggc ttctaggatg
     3841 gagaaaagaa gtctagtaga gttattgaaa gggggcatct tatgattgag ttggacaata
     3901 ttaccaaaac cattggagga aaagtgattt tggataactt atctctcagg attgatcagg
     3961 gggatttggt agccattgtt ggcaagagtg gtagtggtaa gtcaaccttg ttaaatttat
     4021 taggtttgat agatggtgat tacagcggac ggtatgagat ttttggtcag acaaatctag
     4081 cggtcaattc taccaagtcg caaacaataa tccgtgaaca tatctcttat ctatttcaaa
     4141 attttgccct gattgatgat gaaacggtcg agtacaatct tatgctggcg ctgaaatatg
     4201 tgaaattgtc caagaaagac aagctcaaaa aggtggaaga gattttagag agagtaggtt
     4261 tgtcagctac tttgcaccaa aaggtctccg agttgtctgg tggcgaacaa caacgaattg
     4321 cagttgctag agccatctta aaacccagcc agctgatttt agccgatgaa ccgacaggtt
     4381 ctctggatcc tgaaaataga gatttggtct tgaagtttct cttagagatg aatcgagagg
     4441 ggaaaacagt cattatcgtg acccacgatg cttatgtagc ccaacaatgt catcgaatca
     4501 ttgaattagg cgagggaaaa tgagttcgtt cagcttcatt tgaccgactg aatactcata
     4561 tttttcagag agaaatagca taaataggcc taggaatgat atttttatgt cattttcctg
     4621 ggtttttctg ttttaaattg aaaaaaaatt acaatttagg cttgacaaag gatgcatata
     4681 gatgtattgt ttatacaaac aaaaggaata aacataaagt aggctttatt atgaataaag
     4741 atgaagaagg tgttgatgac gatgtttggt ttagtgatgc tccccctact atttgcttgt
     4801 agcaacaatc aatcggctgg aattgaagcc atcaagtcca aaggaaaatt ggttgtagcc
     4861 ctcaatccgg attttgctcc atttgaatac caaaaagtgg ttgatgggaa aaatcagatt
     4921 gtgggttcag atatcgaatt agccaaggct atcgcaacag aactaggtgt cgaattggaa
     4981 ctatctccca tgagttttga taatgtactg gctagtgttc aatcaggaaa agccgacctt
     5041 gccatatcag gtgtttctaa gacagatgaa cggagcaagg tgtttgactt ttccattccc
     5101 tactatactg caaaaaataa actcattgtc aaagaatctg acttgactac ttatcagtct
     5161 gtaaacgact tggcgcagaa aaaggttggc gcgcagaaag gttcgattca agagacgatg
     5221 gcgaaagatt tgctacaaaa ttcttccctc gtatctctgc ctaaaaatgg gaatttaatc
     5281 acagatttaa aatcaggaca agtggatgcc gttatctttg aagaacctgt ttccaaggga
     5341 tttgtggaaa ataatcctga tttagcaatc gcagacctca attttgaaaa agagcaagat
     5401 gattcctacg cggtagccat gaaaaaagat agcaaggaat tgaaagaggc agtcgataaa
     5461 actattcaaa agttgaagga gtctggggaa ttagacaaac tcattgagga tgcctttaaa
     5521 gcgtccattg aaaaatagaa agaaggaaaa gaacatgagt aaagaaaaag taattttggc
     5581 ctattctggc ggtttggaca catcagttgc tattacctgg ctgaagaaag attatgatgt
     5641 tgttgcagtt tgtatggatg tgggtgaagg gaaagactta gatttcatcc atgataaggc
     5701 tctcaaggtt ggggcagtcg aatcttatgt cattgatgtt aaggacgaat ttgctacaga
     5761 ttatgtgcta gtggctcttc agtcacatgc ctactatgaa caaaagtacc ccttggtatc
     5821 tgccttgagc cgccctctta tttctaaaaa attggttgaa atagcgcatc agacaggagc
     5881 gactacaatt gctcatggtt gtacaggtaa ggggaatgac caaatcgaat accagattgc
     5941 agtcgctaag aaagcgaatg aagctaaaaa gtgatggagt gatgggttca gaataagagg
     6001 ttggtcgatt ggcagcctct ttcttgtcgt tgaaaaagtg agatatattg acttttgaaa
     6061 aaaatgtcat aattgagttg ggataaacgt aaaagtcata ctttctcgtt aaatcgttcg
     6121 gcgattcatg gtgacatgga cacatagtag taagcacact atgtggctta gcagagctaa
     6181 aaacttctgg tttaacaatg gctggtatta agaactaacg aacaaataat atgtaaagag
     6241 tcgaggaaat ccctcgactt tttttataat ttctttggtt gataatgggt ctctacttct
     6301 tttttgagat gagacagcat agaagtttcc tcggtcagct ctaggagtga gaaacctttt
     6361 tggataagtt tagcattatc tgtacaaatc ccaatacgga catagcagat agccagccta
     6421 tcgtagcttt tcttattctt ggcgtcctct agtaaagtgt agcagatttg cttacacatg
     6481 tttttatctt gattgtatag atagagtgtg gaaaggttga gcaggattgc tattcgcaag
     6541 tcatataaat gttgatagtt tttatagact tctaggcgtt gcaatatttt tccagtgatg
     6601 agatggaggt attcaatggg aaaactgaaa agaatggtat tgaggatttt tagatcactc
     6661 tcataccatg tatcttgttt ttcaattttt ctccaaagtt ttttgacaac ctgttctgtg
     6721 tgatctgata gttcccttgc cccgtgttga cggatataaa tgacaacttc aagcatatct
     6781 ctgatttctt ctatagggag atcgtggtgg gtcttgaggt agtcttggca tttttgaaat
     6841 aaattgacaa gatcgctggt cccgataatg gaacgcatat tgagataggt ttgcataatc
     6901 tctgtgcgtt gacttggttg ataaagttga cagatatatt caaactcttc aaaactcata
     6961 ttgatttgac ggagaagaaa ttccatattt tcatattttg gggttgtctt gccactttcg
     7021 atttttgata agctggttct tgagagaaca cccccacaga cctcttcttg ggtcagcccc
     7081 tttgactcac gtatttcttt atagaccttt ccgaaatcat aacgcatgat tttctccttt
     7141 tcgtgaaaaa agttaacaaa caataaaatt ttgttaaaaa tattgtatca tagcaatgta
     7201 aaaagtaaaa aagaaagtcg aaataaattt gtgaaaaaag ttaacgtaat tttaaaatga
     7261 gcttaataag aggtactatg taggtagaaa ataaagatag gagaaaaagt atgaataaaa
     7321 aaatattttg tattctagta tgtattatat tattgattag tctggctata atctttccat
     7381 ggggctggcc gatataatgt aagggagttt gagttgttaa aggaggctca gtccttgggc
     7441 aaaaatctgc tgtactacaa tgcttggaaa gatatcaaga acaaagaatg atacggttga
     7501 aaataaatta attcaagaac tactgggcag tgattgaggt ttgggtgaca tcaaagaaaa
     7561 gttagattaa ttctaaagaa ttgagtattt ctatgttaaa aaaatatcct tgtacgatgc
     7621 aacatgacca gtcagattgt gctgcggcag ttgtttcaac agttctttta tcttacaaaa
     7681 aggaactatc aatcatgaag attcgggaaa ttattggtac agacatgtat ggaacgactg
     7741 tcagtggtat tgtttcaggt ctgaataagt tgaattttac agtaaaagct gttcgagtag
     7801 cactggaaga tttgactcca aaattaacat ttcctgcgat tcttcaagtt aagaatgatt
     7861 taggtcaaaa tcattttgtg gtattacata gtataaagga gaaaataaat ggaactcgta
     7921 ttaccaaata attatgttgt gattgatgaa gaagagatga tgtaccttga tgggggggct
     7981 atatatatac ccaggtgggc aattacagga gccattactg gtgcagcata tgcagcatta
     8041 gcagcagcag gaggtggagg ccttcaacta gttcttgcat cttatggatt acgctccgca
     8101 ctggtagctg ggattgttaa aggtttagga gtattaggaa ttcatattgg aaatgctttt
     8161 gcaaatactg ttattagaag tattgcatct gctggaattg gtgctggagc tgattggatt
     8221 tttaccaata ttattgatgg ctgggatggg cgacgtgata atcaattgag aataggataa
     8281 tagtattatg atggttgttt taatttcagt tattgttgca ttcctgtacc ataaaattac
     8341 agagtatgtc aacaacagga aaattgatat tgttattttt ctatcattgc tgatttctca
     8401 ttttatttgg aattactatt ttttaccaat tgaaata
//
LOCUS       DAVMAF010000080         8282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMAF010000080 DAVMAF010000000
VERSION     DAVMAF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.081"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            59..244
                     /gene="briC"
                     /locus_tag="VYO97_001778"
     CDS             59..244
                     /gene="briC"
                     /locus_tag="VYO97_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1754535.1"
                     /translation="MTATETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            607..984
                     /locus_tag="VYO97_001779"
     CDS             607..984
                     /locus_tag="VYO97_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1754536.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(1034..1456)
                     /gene="nusB"
                     /locus_tag="VYO97_001780"
     CDS             complement(1034..1456)
                     /gene="nusB"
                     /locus_tag="VYO97_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1754537.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAINEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1449..1838)
                     /locus_tag="VYO97_001781"
     CDS             complement(1449..1838)
                     /locus_tag="VYO97_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1754538.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1860..2420)
                     /gene="efp"
                     /locus_tag="VYO97_001782"
     CDS             complement(1860..2420)
                     /gene="efp"
                     /locus_tag="VYO97_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1754539.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2549..3991)
                     /gene="gatB"
                     /locus_tag="VYO97_001783"
     CDS             complement(2549..3991)
                     /gene="gatB"
                     /locus_tag="VYO97_001783"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1754540.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3991..5457)
                     /gene="gatA"
                     /locus_tag="VYO97_001784"
     CDS             complement(3991..5457)
                     /gene="gatA"
                     /locus_tag="VYO97_001784"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1754541.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLEDIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(5457..5759)
                     /gene="gatC"
                     /locus_tag="VYO97_001785"
     CDS             complement(5457..5759)
                     /gene="gatC"
                     /locus_tag="VYO97_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1754542.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5972..7516
                     /locus_tag="VYO97_001786"
     CDS             5972..7516
                     /locus_tag="VYO97_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1754543.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7771..7968
                     /locus_tag="VYO97_001787"
     CDS             7771..7968
                     /locus_tag="VYO97_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1754544.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            8079..8255
                     /locus_tag="VYO97_001788"
     CDS             8079..8255
                     /locus_tag="VYO97_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1754545.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
BASE COUNT         2303 a         1596 c         1875 g         2508 t
ORIGIN      
        1 cctgatagag ttgttcacat cttatttcaa atcactatat tttaagaagg tacttgcaat
       61 gacagctaca gaaacattta cagttatttc aactgaggac ttggagcaaa cttcaggtgg
      121 tcttgctgtt tgggaagatg gatatagtag atggttatat tatagagaat ttgctcctta
      181 tatgaggcaa ggggcactta attcttatat agatgcttgg aagtacggct tccgagcagg
      241 gtaatctttc aaaaaagtac gatgagaaac acaaaaaaac taagacaatt tggaatattt
      301 ttgcttatta ttttactatc tacctattta ccacaaacaa ttggattgta tgtgacgatc
      361 attttagggt taggagctga tgtctattct cttattttaa caatggtatt agtgggaagt
      421 ttccttctat taatttggag gttaaaaaag aaaaagatgc tctttatctt tgaaaaaaag
      481 agctggaact ggtcatttgt cttctactta tttgcaacgt atgtggttta tcagattctc
      541 ggtaattttt aggcacgcta tgctcatttg attaaccata ggaatattca tgatgagtat
      601 tttaccgtgg ttctttcaaa tggacaacca acctttttat cgactatctt atcttttgtc
      661 ctgcttgtaa tcatcggtcc tgtttttgag gagactcttg atagagggta ttttatgaat
      721 accttctttc ctaagtcaaa gtactattta gatgtcatct tatcaggcct tatctttggg
      781 attagccatt tgatactatc tcacagagat ccaattagtt tattatatta tagtctgatt
      841 ggtttctttt ttgcccttgt ttatcgttct accgacaatc tgagattaac gattctctgc
      901 catagttttt ttaactttct caatcatgca aaacctatct ggatttttgt ttacaattat
      961 atctattatc atttttttag atagtgggag gcaaagaaaa aagtgtttga tatctatcaa
     1021 acactttttc tttttattgt tcttctgtta caaactggct gagcagtcca ttgataaaac
     1081 gggcagattt ttgatcggag aagtcctttg caagctcgat agcttcatta atagcaacca
     1141 gctgaggagt gtcaaatgaa gtgatttcaa agactcccaa gcgaaggagg tttctctcca
     1201 cgagcgttaa gcgttcaatg gtccaacctg cttttaaatg ctgagtgatt tgcttatcta
     1261 gttcttcctt tttagcttga acaccagaaa cgaggtctat caaaaaggct ggaagttgta
     1321 catccgtata ttcacgatca tgagtatagg cgaaacgaca agcagtttcg acatccgtac
     1381 cgaactcaag gctcatgaga gcttgaaaag cgcatttacg gagttggcgt ctagattcta
     1441 atagtggact agtcattgag gaagtcctcg tcaaatagat ctttcaattc tggttttggt
     1501 gttttatctg ggacgatacc tgcaacgtga atattgatag cagcgagttc tacatcagcc
     1561 atattacgga cggcatcttt gacagctttc tggatagcaa ccgctacctt aggaactttt
     1621 actccgtact caaggtagag atagatatct gctgtgagtt cttcgtccac gtttttaaga
     1681 taaatgccac ggccgagtga aagttttgaa agggtatcag acactgatct gtttgaaaaa
     1741 gagtgaacac cctctacctt tgcagtagcg atagcaatga ttttttcaag tacacgtggg
     1801 gcgataacga tttcgccaag ttgttcttca attcccatag aatgacctct ttctagagat
     1861 taggcacgag aaacgtaagt tccttctgca gtgttgataa cgagtttttg tcctgcttcg
     1921 atgaagtctg gaacgtttac gacaagtcca gtttccatcg ttgctggttt gccagaacct
     1981 gtaacagtag cacctttgat agatggttga gtttcagcaa ctgtcaactc aacagtagta
     2041 ggaacggtga caccgatcac ttcagttccg tagaattgga ttttcacatc agagttttca
     2101 aggatgtaaa gcaattcgtt ttcaacattg actacaggga tttcgtactg gtcgtaagtt
     2161 tctgtattca tgaagtatgc tgtgtcatcc attttgtaca agtattgagc tgggacagtc
     2221 tcgataatag cttgttcaaa tttttcctct ggacggtagc ttgtgtcaaa tgtagaacca
     2281 gtacggacat cacgcaattt catacgcatg atcgtgtttc ctttacctgg cttgtggtga
     2341 ctagcttcca aaacgcgaat caatttgccg tcagctgttt caaaggtcat accagctttt
     2401 aatttacttg cttcaatcat gtttttacct ctttaataaa tattattact cttcatttta
     2461 ccataaaata ctctggaaat aaagccaaaa tagttattgg gagttggtag agaagaaaaa
     2521 accaacctta gggctggttt tgttctgtct agttttcttt caacttcgcc aattcctgtg
     2581 caagtagttt aagggcaact tgtgggttgg cttggccttt ggttgccttc ataaggaatc
     2641 ctgtaaaggc cttgtcggcg ttacgtttgc ctgacttgaa gtcggcaaca gcagcttcgt
     2701 tatcggcaaa gacttggtgg atgattggga tcaagatagc tggatctgaa atttgaacca
     2761 tacctgcttt ttccacgtat tcacgcgcgc caccgccatt tttagctaga tggacaaaga
     2821 ctttcttggc aatcttagat gaaatagtac cgtcttcgat gatggcaatc atttcaacca
     2881 agttttctgg tgtcaattcg atttgttcca gtgttttacc ttcagcattc aagaactgag
     2941 cgacttcccc ttggagccag ttagagactt gtttggcatc accacctagg gcaacagctt
     3001 tttcaaagaa gtcagaagtg actttattag cagtcaactg actagcatcg tagtctgata
     3061 aaccaaggtc agatacataa cgcgcacgac gttcttttgg aaactctggc aactcagtcc
     3121 gcatttcctc aatccactcg tcagaaattt caaagagggg taggtctggt tctgggaagt
     3181 agcggtagtc agcagcccct tccttgacac gcatgaggat ggttgttttg ttagcttcat
     3241 cgtaacggcg tgtttcttgg cggatttgac cacctgagcg aagaatttca gcctggcgtt
     3301 ggacttcgta ttcaagacct ttacgaacgt ttgagaagga gttgaggttc ttcaattcag
     3361 tcttggtacc gaatttttct tgaccataag ggcgaaggga gatgttggca tccacacgca
     3421 tagaaccttc ttccatctta acgtcagaaa tgccagcgta ctggataact tccttgaggg
     3481 ctgtcagata agcataggcc tcctctggcg aacgcatatc ggcttcagat acaatctcaa
     3541 tcaaaggtac cccttggcgg ttaaggtcaa cgtaagagta gccgtctgta ccgtgggtgt
     3601 ttttaccagc gtcttcctct aggtgggcac gttcgatacc gattttcttg gtcgtaccgt
     3661 cttctagctc gacttcaatc cagccgttat aaccgattgg ctcatcaaac tgagaaattt
     3721 ggtaggcttt gggattatca gggtagaagt agttcttgcg gtcaaagtgc atttttttgt
     3781 ggaggtccat gttgagggca agagcagcct tgataccggc atcaacaacc cctttattga
     3841 gaactggtag aactcctggg aaagaccagt caatcacgtt agtgttggca ttttggtcat
     3901 ttccaaagtg ggcagaagta ggtgagaaga tttttgaatt ggtgttgagc tctacgtgga
     3961 cttcaagtcc gatgactgtt tcaaagttca ttagttgtca cctccaaaaa tcacgggttg
     4021 ttgtttgtgg tagtctgttg ttgcttcaaa agcagcagca gcttggtaaa tggtttcctc
     4081 agagtacttg ggaccaatca attggagtcc gacaggtaga ccttgagaga atccagcagg
     4141 aatcgaaatt ccaggcagtc ctgccaagtt tacaggtatg gtcaagaggt cggctaagta
     4201 catggcaact gggtcatggt tgagagaatc caagtcatag gcaacacttg gagcagttgg
     4261 acccaaaatc aaatcgtaat ccgcgaagac tttttcgaaa tcttgaatga taagtgtacg
     4321 gacctgtcca gccttcttgt agtaggcatc gtagtaacct gatgaaagac tgaaagtacc
     4381 cagcatgata cggcgcttca cttcttcacc aaaaccttgg ctacggctgt ttacatagat
     4441 ttcatcaaga ttagtcgcat cttctgcgcg gtagccgtaa cggataccgt caaaacgttg
     4501 caagtttgat gaagcttctg atgaagcgat gatgtaataa acggcaacac cgtatttaga
     4561 gtgaggaagg ctgacttctt cgacgatagc acccaatttt tcaaagtgtt tagcggcatt
     4621 tagaatggtt tccttaacct ctgggttaat tccttcaccg aggtattcct taggcaaagc
     4681 aattttcatg cccttgatgt cttggccgat ttttgaagta aagtcggcga tgcggacagg
     4741 agcagaagta gagtctttag catcttcgct ggcaatagcg ttgagcaaga gggcgttttc
     4801 cttaacagta ggagcaaaag gtccaatctg gtctaatgag ctaccaaagg caatgagacc
     4861 gaaacgtgaa actgttccgt aggttggttt gagaccaacg attccgttga aggcagcagg
     4921 ttggcggatg gaaccaccag tatcagaacc aagtgacaag cgaacttgtc ctgaggctac
     4981 agctgcggca gaaccacttg atgacccacc aggaaccttg ctgtggtccc aagcgttttt
     5041 agttgctccg tagtgtgaag tttcacctga accacccata gcaaattcgt ccatgttggt
     5101 ctttccaacg acaatcatgc ccttggtttt tgcattggca acagctgtcg catcaaagat
     5161 tggctcatag ttgtagagca tttttgaggc agcagttgtg agaataccgt ctgtagagat
     5221 gttatcctta acagcaagtg gaattcctga aaggacattg tcagcatcaa ttccagcttc
     5281 atcaatggct ttagcttgaa caagagcttg ctcctcagcg atggtgacaa atgaattgag
     5341 ggcttcctca cgagacttga tatcttcaag tgttgcttgg gtcaattctg ttgcagaaat
     5401 ttccttagaa acaaggagat tgtgcaactc ttcaatagtt ttattgttaa aagtcattag
     5461 gcatctcctc catcgtctag gatagctggc accttgatat agtagttgtc tttttcaggt
     5521 acgtttttaa acaagcgatc acggtctgtt ccttcttcgg ccacatcagg gcggagtaca
     5581 gtcttgcggt cagccatagt cgtagtaggt gcgacaccag ttgtgtcaac ttcgcccagc
     5641 aattcaacca tgtcaacaat cttagacaag gtggtcgcaa aggcagcagt ttcttcttca
     5701 gagaatctta attttgaaag attggcaacg tgtgttacct cttcttgcgt aattttcatc
     5761 ttgcatcctt tcgtgaaatg atgattttta gtctgttcta ttttaccata ttttcccata
     5821 aataagggag ggggaagtga aaagaaatag aaattgaaaa aaatgctaaa atccgatata
     5881 atggagtgtt gaaaggaatg gtaaaatcca atgtaaaaat cattctcagc tattgaaaca
     5941 agaaaaatag agaatcaaag aaagagaact tatgaatatt caagaagaaa ttaagaaacg
     6001 tcgtaccttt gccattatct cccacccgga cgcggggaaa acaaccatca ctgagcagtt
     6061 actctacttt gggggtgaga ttcgtgaggc tggtacggta aaagggaaga aaacagggac
     6121 ttttgctaaa tctgactgga tggatatcga gaagcaacgt gggatttctg ttacttcatc
     6181 tgttatgcaa tttgactacg acggcaagcg cgtgaatatc ttagacacgc cagggcacga
     6241 ggacttctca gaagatacct atcgtacctt gatggcggtg gatgctgcgg tcatggtcgt
     6301 ggactctgcc aaggggatcg aggctcaaac caagaaattg tttgaggttg tgaaacatcg
     6361 tggcattcca gtctttacct ttatgaacaa gctggaccgt gacggtcgtg agcctttgga
     6421 tctcttgcaa gaattggaag aaatcttggg cattgctagc taccctatga actggcctat
     6481 cgggatgggg aaagcctttg agggcttgta tgacctctat aaccaacgtt tagagcttta
     6541 taaaggggat gagcgttttg ccagtctaga agacggggac aaactctttg gtagcaatcc
     6601 tttctacgag caagtcaagg atgacattga gcttttaaat gaagctggga atgagttttc
     6661 agaggaagct attctggctg gagaattgac gcctgtcttt ttcggttcag ccctgacaaa
     6721 ctttggtgtg cagaccttcc ttgaaatctt cctcaagttt gctccagaac cacatggtca
     6781 caagaaaaca gacggtgaaa ttgtggatcc ttatgacaag gatttctcag gctttgtctt
     6841 taaaatccaa gccaacatgg atcctcgtca ccgtgaccgt attgcctttg tccgtatcgt
     6901 atcaggcgaa tttgagcgcg gcatgagtgt caatctccct cgtactggta agggggctaa
     6961 actatctaat gttacccagt ttatggcgga gagtcgtgag aatgtgacca atgccgtggc
     7021 aggtgatatt atcggggttt acgataccgg tacttatcag gttggggata ccttgacggt
     7081 tggaaaaaac aagtttgaat ttgaaccact gccaaccttt actcctgaaa ttttcatgaa
     7141 agtttctgct aagaatgtta tgaagcaaaa atccttccac aaggggattg agcaattggt
     7201 gcaagaagga gccgttcagc tttataagaa ttaccaaaca ggtgagtaca tgctgggagc
     7261 tgttggtcaa ctccagtttg aagtctttaa acaccgtatg gaaggcgaat acaatgcgga
     7321 agtggtcatg agcccaatgg gtaaaaagac cgttcgttgg atcaagcctg aggacttgga
     7381 tgaacggatg tcgtcaagtc gcaatatctt agccaaagac cgttttgacc aaccagtctt
     7441 tctctttgaa aatgactttg ccctccgctg gtttgcggac aagtatccag acgtagagtt
     7501 agaggagaag atgtgactca gtaccaacaa ttggaactaa agttccaaat tgttggacgc
     7561 tagtcgctat ttggcgaact agctaactgc ctcactaact acgtttggca aatgaaatcg
     7621 atttgtaaaa ctccgtgtcg taatgtaaaa attgtaaacc gtaatgggac ttcatctaaa
     7681 atctccactg gtatggtttt acgaacgaat aggcgaaaat aatggttacg tagaaacata
     7741 tgatgggaat aaaaagaaat gagataaaaa gtgaaagaag agaaaaaagc gattgtctta
     7801 ggtgcagata atgcttatat ggataaagtt gaaacaacaa taaaatctct ttgtgttcat
     7861 cattataatc ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg
     7921 atggaaaaaa gactagagac tttgaatagt gagattgtga atgtttagat tgacagtaat
     7981 attctgaaag gttatcgtct cccctttgag ggactttcat atgcggcttt tttaggtatt
     8041 ttattccaaa gtatgtaagc gagtctcgtg tactgtattt ggattcagat attgttgtaa
     8101 gaaaatcgat agatgagctg tgggatttgg acttgacggc tataccctta gctgcagtta
     8161 gagatgattt ttatacacat aattttaatt ctggtgttct gttaatcaac gatggcatgt
     8221 ggagagcaga aaatgtcacg caagacctga tttaagacac aaataaaaaa tcttccaatt
     8281 cc
//
LOCUS       DAVMAF010000081         8083 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMAF010000081 DAVMAF010000000
VERSION     DAVMAF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.082"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            17..970
                     /locus_tag="VYO97_001789"
     CDS             17..970
                     /locus_tag="VYO97_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1754546.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLEFIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1047..3035
                     /gene="uvrB"
                     /locus_tag="VYO97_001790"
     CDS             1047..3035
                     /gene="uvrB"
                     /locus_tag="VYO97_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1754547.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDVNSLNKQE
                     RKDLVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3058..3525
                     /locus_tag="VYO97_001791"
     CDS             3058..3525
                     /locus_tag="VYO97_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1754548.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFVESVIREIYEETGLTIQNPQLVGVKNWPLDTGGRYIVFCYRATEFSGTLQS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(3522..3608)
                     /locus_tag="VYO97_001792"
                     /pseudo
     CDS             complement(3522..3608)
                     /locus_tag="VYO97_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3839..4816
                     /locus_tag="VYO97_001793"
     CDS             3839..4816
                     /locus_tag="VYO97_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1754549.1"
                     /translation="MTTIKQVAEEAGVSKSTVSRYISKKGYVGDDAREKIKNAIKKLN
                     YTPNVLAQSLKTKKNQMVGLLLPDISNPFFPRLVRGAESYLKDKGYRIMVGTISDHDS
                     LEEYINLLLKTNAAGIITTLDFTKEFPNLTLPVVVVDRISKDTGYGVFSDNQLGGRLA
                     AKAIWNAGAEQVMIIKVLDDKAENIVERFEASLNYLRNKNLEICIEESETFDFEKIQK
                     EAKENLKRNPNIDSIIAPSDIHAIAYIHEILAIGKKIPDDIQIIGYDDIVLSQFIYPS
                     LSTIHQSSYKMGEQAAKLIYNMANKFSIDEAKIKLPVRYIERNTLRRKK"
     gene            4813..5724
                     /gene="rbsK"
                     /locus_tag="VYO97_001794"
     CDS             4813..5724
                     /gene="rbsK"
                     /locus_tag="VYO97_001794"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV1754550.1"
                     /translation="MSKIVVVGSISMDLVMRTKRIPEGGETIFGDSFNIVPGGKGANQ
                     AVAIGRLSSVEDNIYIFGNVGEDIFSADLLSNLQNNDISTAYVGTVPQSTGVAQITLY
                     GGDNRIIYYPGANNLVKTNDWKNEWKLLSDASIVVLQNEIPHEANLSIAKFCQENQVK
                     VLYNPAPARETDKEMIPFCDFITPNEHECSELFPDKKLEEIIKIYPNKMIVTLGVEGS
                     IYYDGTAVKKIPAIKAEVVDTTGAGDTFNGAFAYAISKGKEMNVALSFATIASHLSVQ
                     RFGAQGGMPSLKEIKEHSGYEEIWDFK"
     gene            5699..6097
                     /gene="rbsD"
                     /locus_tag="VYO97_001795"
     CDS             5699..6097
                     /gene="rbsD"
                     /locus_tag="VYO97_001795"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEV1754551.1"
                     /translation="MKKYGILNSNIAKLADDLGHMDWVCIGDLGLPVPKGVDKIDLAL
                     RKGSPSFLEVLKEYSDHVLIEKIFLAEEIKEQNTEQWQAVLDLLGSNIIIEYISHEEL
                     KAMNTKVKAVIRTGEDTPYSNIILQSGVII"
     gene            6111..7589
                     /locus_tag="VYO97_001796"
     CDS             6111..7589
                     /locus_tag="VYO97_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV1754552.1"
                     /translation="MKIEMKNISKSFGNNRVLESIDLVLNSGEVHALMGENGAGKSTL
                     MNILTGLFPATSGTIFIDGKEKTFSNPQEAERFGLSFIHQEMNTWPDMTVLDNLFLGR
                     EIKNSIGFLDKASMEKKAKEAFKRLNISIPLDAIIGQLSVGQQQMIEIAKCLLSEVSL
                     LIMDEPTAALTDRETETLFKVIEGLKSDGVGIVYISHRMEEIFKITDLITVMRDGFVI
                     DTKKTKLTNADELVQKMVGRELEDYYPEKKAEIGNIVFQAKNLSGNSFTDVSFYVRQG
                     EILGFSGLMGAGRTEIMRAIFGIDSLKSGQIIINEEELIIKNPFEAIKHGIGFLTEDR
                     KDEGLILDFSIKDNMTLPSTRDFVKNGIFDNKTSDIFVQRLIDRLQIKSGYPDKEVGT
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELAERGVPIIMVSS
                     DLPEVIGVSDRIMVMHEGRISGELTRQEATQEKVMQLATGGQ"
     gene            7591..>8083
                     /gene="rbsC"
                     /locus_tag="VYO97_001797"
     CDS             7591..>8083
                     /gene="rbsC"
                     /locus_tag="VYO97_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEV1754553.1"
                     /translation="MKNTMKYMSELTTVIALIILMAVITIINSNFLTANNLLNLLLQV
                     TSNALIAFGMTFVILTGGIDLSVGSILALSSALTAGLLGSGMPVTLAILISLILGCIL
                     GMMNGLLISYGKLAPFIVTLATMTIFRGATLVYTNGNPITKGLSDTFLFQFLGQGYIV
                     GIPF"
BASE COUNT         2628 a         1260 c         1718 g         2477 t
ORIGIN      
        1 aaaagaggac ggggatatga agaacaaaag aatatttaaa gacttccaag cttcaaaaat
       61 gagtttaaac atttacacaa gccccttgtt agcctttgtt tttgtcttca taggagagtt
      121 tgtggctttt actttgtatg gtattggctt gttagctctc atcggacttg ctagaaattt
      181 tggagaggct ggtcaaaatc ttgcaagcta cttgcagacc ttgcatcaga gcttgacgga
      241 taaaacaagt gactttcgtt taattttagg attactggcc tttggtttta ttcttaacac
      301 tgtgttcaga tggacaagaa aagttgagaa aagacctatt cgaaccttgg gattttatag
      361 agagaatttc ctcagcaatc ttctgaaagg atttagtcta ggtctggcac tttttcttct
      421 gaccttgtta ggtttggtgg tcttaggtca atatcgtttg gaattcattc acttgaatcc
      481 ttattctctt gcctttgtcg tctttactat cccattttgg attttacagg ggacagcaga
      541 agaagtggtg gcccgtgctt ggttacttcc tcaattggcc tcaagaacca atctaaaact
      601 agctattctt atatctagcc tgttctttac cctgcttcat atgggcaatt ctggcctcac
      661 ccctctatct ctagtaaatc tctttttatt cggagttgcc atggctcttt accttctcaa
      721 aactgataca gtttggggtg ttgcaggtat tcatggtgct tggaattttg ctcagggtaa
      781 tctctttggg attttagtta gtggtcaacc gtcaggaacg tctctgatga cctttttacc
      841 acaaggcaat caagattggc tatcaggtgg ttcttttggc atagaaggtt ccattatgac
      901 aagtctggta ttactactgc tgattgtcta tcttgctaat aaattaaaga aagaaaatga
      961 aaggatgtga cttcggtcca tccttttctt cgtgaaaata ctataagtat gctaaaatag
     1021 gaatagcaca tggagagagg attcttatga tcaatcacat tacagataat caatttaaac
     1081 tagtatcaaa atatcaacca tcaggagatc aaccccaagc tatcgagcag ttggtggata
     1141 acattgaggg gggagaaaaa gcacagattc tgatgggggc gactggaaca gggaagacct
     1201 atactatgag tcaggtcatt tctaaagtca ataaaccaac tctggttatt gcccacaata
     1261 aaactctggc tggtcagctc tatggggagt ttaaggaatt tttccctgaa aatgcagttg
     1321 agtatttcgt atcctactat gattattacc agccagaggc ctatgtccct tctagcgata
     1381 cctatattga gaaggatagt tctgtcaatg acgagattga caagcttcgc cactcagcta
     1441 cctcagccct tttggagcgt aatgatgtta ttgtcgtggc ctcagtctct tgtatctatg
     1501 gtttgggttc gcccaaggaa tacgctgata gtgtcgttag tctccgtcct ggtctagaga
     1561 tttctcgtga taaactcttg aatgacttgg tcgatattca gtttgaacgc aatgatattg
     1621 atttccaacg cggaagattt cgcgttcgtg gggatgtggt agagattttc ccagcttccc
     1681 gagatgaaca tgcctttcga gtagaatttt ttggagacga aattgaccgt attcgtgaag
     1741 ttgaggctct gacaggtcag gtgttgggag aagtggatca tttagcgatt ttcccagcga
     1801 cacactttgt gaccaatgac gaccacatgg aagttgccat tgcaaagatt caggccgagt
     1861 tggaagaaca attagctgtc tttgaaaagg aaggtaaact gcttgaagcc cagcgtttga
     1921 aacagcggac agagtatgat atcgaaatgt tgcgtgagat gggctatacc aacggggttg
     1981 agaactattc acgacacatg gatggacgga gcgaaggaga gcctccttat acgctcttag
     2041 atttcttccc agatgatttt ttaattatga ttgacgagag tcacatgacc atggggcaaa
     2101 tcaagggcat gtacaatgga gaccgttcgc gtaaagaaat gctggttaat tatggtttcc
     2161 gtttgccgtc tgctttggac aatcgtcctc tccgtcggga ggagtttgag agtcacgttc
     2221 atcagattgt ttacgtttca gcgacacctg gtgactatga aaatgaacag accgagacag
     2281 tgattgagca aatcattcgt ccaacgggac tcttggatcc agaggtggaa gtccgtccga
     2341 ctatgggaca gattgatgac ctcttgggtg aaatcaatgc ccgcgttgaa aaaaatgagc
     2401 gtacctttat cacaactttg accaagaaaa tggcagagga cttgaccgac tacttcaagg
     2461 aaatgggtat caaggtcaag tacatgcact cggatatcaa gaccttggaa cggacggaga
     2521 ttatccgtga cctgcgcttg ggtgtctttg atgtcttggt cggaattaac ctgctccgtg
     2581 aaggaattga cgttcctgaa gtgagcctcg tagctattct cgatgctgac aaggaaggtt
     2641 tcctacgtaa cgagcgtgga ctcattcaga ctattggtcg tgctgcccgt aacagtgaag
     2701 gtcatgtcat catgtatgct gacacggtta cccagtctat gcaacgtgct atcgatgaaa
     2761 ctgcccgccg tcggaaaatc cagatggcct ataatgaaga acatggtatc gttccacaaa
     2821 ccattaagaa agaaatccgt gacctgattg ccgtaactaa ggcagttgct aaggaagaag
     2881 acaaggaagt tgatgttaat agcctcaaca aacaagaacg taaagatctt gttaagaaac
     2941 tcgaaaaaca aatgcaagaa gccgtcgaag tgcttgactt tgaactagca gcacagattc
     3001 gtgatatgat gttggaagtc aaggcgttag attagaataa tatctaaagg agaaaatatg
     3061 tcccgttccc aattaacgat tttaacaaac atttgtctga ttgaagacct cgaaactcag
     3121 cgcgtggtga tgcagtatcg cgcccctgaa aacaatcgct ggtctggtta tgcctttcca
     3181 ggaggccatg tagaaaatgg cgaatctttc gtagagtctg tcattcgtga aatctatgaa
     3241 gagacaggat tgactatcca aaatccacaa ctggtaggcg ttaaaaattg gcctctggat
     3301 acaggcgggc gctacattgt cttttgttat agggcgaccg agttttctgg aactcttcaa
     3361 tcttcagaag agggagaagt ttcttgggtg caaaaagacc agattccaaa cttggatctg
     3421 gcctatgata tgttaccctt gatggagatg atggaagctc ctgacaaatc agagtttttc
     3481 taccctcgcc gtacagaaga tgattgggag aagaaaatct tctagtcttt tactaaataa
     3541 cctagctgat ccaagacctc ctcaatatag tggaggtctt gttgtgtttc gtatctaata
     3601 gtatagtaga ttgaaataag atataaacaa atcaatcagg aaagtaaaat taatttctag
     3661 aaatatttta gcagtcacga cgtactattc tggtttcaat caattataat agaagattag
     3721 taaaaacatg tgtactctga aataaattta aaatatttct aaaaaagtat tgacaaaata
     3781 aaagaaaacg cttactatat aaataaggga acggttccac aataaaaaag gaggagtgat
     3841 gactacaata aaacaggttg ctgaagaagc tggagtatca aagtcaacag tttcaagata
     3901 tatttcgaaa aaaggctatg tcggagatga tgcgagagaa aagataaaaa atgctatcaa
     3961 gaaattaaat tacacaccaa atgtattagc acaatcttta aaaactaaaa aaaatcaaat
     4021 ggtaggactc ttattgccag atatttctaa tcccttcttc ccaagattgg ttcgaggagc
     4081 cgaatcatat ttgaaagata aaggttatag gattatggtt ggtactattt cagatcatga
     4141 ttcgttagaa gaatatataa accttttgtt aaaaacaaat gcagctggta ttataacaac
     4201 acttgacttt acaaaggaat ttccaaatct aacattacct gttgttgtgg ttgaccggat
     4261 tagtaaagat acaggctatg gtgttttttc tgataatcag ttgggtggac gtttggctgc
     4321 taaagcaatt tggaatgctg gcgctgagca ggttatgatc atcaaagttt tagatgataa
     4381 agctgagaat attgtggaac gatttgaagc aagtttaaac tacttgagga ataaaaattt
     4441 agaaatttgt attgaagaaa gtgaaacgtt tgactttgaa aaaattcaaa aagaagctaa
     4501 agagaatcta aaaagaaatc caaatattga tagtattata gcaccttctg atattcatgc
     4561 gatagcttat attcatgaaa ttttagcaat tgggaaaaaa attccagatg atattcaaat
     4621 cattggttat gatgatattg tacttagtca atttatttat ccttctttat caactatcca
     4681 ccaatcatca tataaaatgg gagaacaggc tgcaaagcta atctataata tggcaaataa
     4741 gttctctata gatgaagcta aaattaaact ccctgttagg tacatagaaa gaaatacatt
     4801 aaggagaaag aaatgagtaa aattgttgtt gttggaagca tttcaatgga cttagttatg
     4861 agaacaaaaa ggattccaga aggaggtgaa acgatttttg gcgattcatt taatatagtt
     4921 ccaggtggaa aaggtgcaaa tcaagctgta gctattggta gattatccag tgttgaagat
     4981 aatatttaca tatttgggaa cgtaggagaa gatatttttt cagcagattt attaagtaat
     5041 ctgcaaaata atgatatttc tacagcatat gtgggaacgg taccacagtc tacaggagtt
     5101 gcacaaatta ctttatatgg aggtgataat aggattattt attatcccgg agctaataat
     5161 ttagttaaaa caaatgattg gaaaaatgag tggaaattac ttagtgatgc aagtattgtt
     5221 gtattacaga atgaaatacc acatgaagcc aacttatcta tagctaaatt ttgccaagaa
     5281 aatcaagtta aggttcttta caatcctgca ccagctagag aaacagacaa agagatgatt
     5341 cctttttgtg attttattac tcctaatgag catgaatgtt cagagctttt tccagataag
     5401 aaattagagg aaattattaa aatttatcct aataaaatga ttgtaacatt aggagttgaa
     5461 ggctctattt attatgatgg gacagcagtt aagaaaattc ctgctataaa agctgaagta
     5521 gttgatacta caggagcagg agatacgttt aatggtgctt ttgcttatgc tatctcaaaa
     5581 ggaaaagaga tgaatgttgc attgtccttt gcaacgattg cttcacatct ttctgttcaa
     5641 agatttggag cacaaggagg tatgcctagt ttgaaagaaa taaaggagca ttcaggatat
     5701 gaagaaatat gggattttaa atagtaatat agcaaaacta gctgatgatt taggtcatat
     5761 ggattgggtt tgtatcggag atttgggatt gccagtccca aaaggtgttg ataaaattga
     5821 tttagcatta agaaaaggta gtccaagttt tttagaagtt ttaaaagaat attcagatca
     5881 tgtacttatt gaaaaaattt tcttagcaga agaaattaaa gaacaaaaca cagaacagtg
     5941 gcaagcagtt ttagaccttt taggatcgaa tataattatt gaatatatta gtcacgaaga
     6001 attgaaagct atgaatacta aagtaaaggc agttattcgc actggggaag atacaccata
     6061 ttcgaatata atcttgcaat caggagttat tatttagaag ggggttacct atgaaaattg
     6121 aaatgaagaa catttcaaaa tcttttggga ataatcgtgt tttagaaagt attgatttgg
     6181 ttcttaattc aggagaagtt catgctttaa tgggagaaaa tggtgcaggc aagtcaacct
     6241 taatgaatat tttaactggt ctttttccag ctacttcagg aactattttt attgatggaa
     6301 aagaaaaaac attttctaat cctcaagaag cagaacggtt tggactgagt tttattcatc
     6361 aagaaatgaa tacttggcca gatatgactg tacttgataa tcttttttta ggaagagaaa
     6421 ttaaaaattc gattggcttt ctagataaag ctagtatgga aaagaaagct aaagaagcat
     6481 ttaaacgtct taatatctct attcctctcg atgcaattat tggtcaatta tcagttggac
     6541 aacaacaaat gatagaaata gcaaaatgtt tattatcaga agtttccttg ttaattatgg
     6601 atgaacctac agcagcttta actgatcgtg aaactgaaac acttttcaaa gtgatagaag
     6661 gattaaaatc tgatggtgta ggtattgtgt atatttcaca tcgcatggaa gaaatcttta
     6721 agatcacaga tcttataact gtaatgagag atgggtttgt aattgatacc aagaaaacga
     6781 agttaactaa tgcggatgaa ttagttcaaa agatggtagg tcgtgaatta gaagattatt
     6841 atccagaaaa aaaggctgag attgggaata ttgtttttca agctaagaat ctttctggta
     6901 attcttttac ggatgtttct ttctatgtac gtcaagggga aattcttggt ttttctggtt
     6961 tgatgggggc tggtcgtact gaaataatgc gagcaatttt tggaattgat tctttaaaat
     7021 ctggtcaaat tataataaac gaagaagaac tgataattaa aaatcctttt gaagcaatta
     7081 agcatggaat tggtttctta acagaagatc gtaaagatga gggattgatt ttagattttt
     7141 ctatcaaaga taatatgact ttacctagta ctcgtgattt tgttaaaaat ggcatttttg
     7201 ataataaaac aagtgatata tttgtacaac gcttaattga taggttacaa attaaatcag
     7261 gttatccaga taaagaagtt gggacacttt ctggtggtaa tcaacagaaa gtagttttag
     7321 caaaatggat aggtattgca ccaaaagtac tgattttaga tgagccaact cgtggggtag
     7381 atgttggagc taagcgtgaa atttaccaat taatgaatga attggctgaa cgaggtgtac
     7441 cgattattat ggtctcttca gatttaccag aagtgattgg agtcagtgat cgtattatgg
     7501 tcatgcatga aggtagaatt agtggagaat taacacgtca agaagctaca caagaaaaag
     7561 ttatgcaact agctacagga ggacaatagt gtgaaaaata ctatgaagta tatgtcagaa
     7621 ttgacaacag taatagcatt gataatttta atggctgtca tcactattat caattcaaat
     7681 tttttaacag caaataatct gttaaattta ctattgcaag taacatcaaa tgcactgatt
     7741 gcttttggaa tgacctttgt tattctaaca ggtggaattg atttatcagt tggatcaatt
     7801 ttagccttat ccagtgcgct aacagctggc ctattaggat ctggaatgcc tgttacatta
     7861 gcaattctca tctctttaat tttgggttgc attctaggta tgatgaatgg attattgatt
     7921 tcctacggga aattagctcc atttatcgtc actttagcga ctatgactat ttttagggga
     7981 gcaacacttg tttatacaaa tggaaatcct attacaaaag gattaagtga tacattctta
     8041 tttcaatttt tggggcaagg ttacatagta ggaattccat ttc
//
LOCUS       DAVMAF010000082         8003 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMAF010000082 DAVMAF010000000
VERSION     DAVMAF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.083"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            5..1405
                     /gene="galT"
                     /locus_tag="VYO97_001798"
     CDS             5..1405
                     /gene="galT"
                     /locus_tag="VYO97_001798"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1754554.1"
                     /translation="MKNRVLALIGEEGIDRISDENDLKQIKDYLVEIALKNGKIKDLI
                     EEKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDYIKTSAIAKN
                     IEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENEGYQGRINYP
                     ARSNHRIIRLKLGDEVWGFQYSPYSYFNKHAIFLNSQHVPMAITSKTFEQLLEIVDIL
                     PGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFCFKDFEDVNAGIVKWP
                     MSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTPIARKVDGRY
                     ELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRLKPELEEVGK
                     YLLGEDNAIADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLEDAGVYKNTP
                     SGHEAFMRFVKSVGIN"
     gene            1418..2371
                     /gene="galE"
                     /locus_tag="VYO97_001799"
     CDS             1418..2371
                     /gene="galE"
                     /locus_tag="VYO97_001799"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1754555.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATNPYGYTKVMLEQILKDVHVADSEWSIALLRYFNP
                     IGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFGNDYDTLDGTGVRDYIHVVD
                     LAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFESVNKIPIPYKIVPRRSGDV
                     ATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNGYN"
     gene            2536..3195
                     /locus_tag="VYO97_001800"
     CDS             2536..3195
                     /locus_tag="VYO97_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1754556.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIYMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3220..4287
                     /locus_tag="VYO97_001801"
     CDS             3220..4287
                     /locus_tag="VYO97_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1754557.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4390..5400
                     /locus_tag="VYO97_001802"
     CDS             4390..5400
                     /locus_tag="VYO97_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754558.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5412..7103
                     /locus_tag="VYO97_001803"
     CDS             5412..7103
                     /locus_tag="VYO97_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1754559.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7106..7816
                     /locus_tag="VYO97_001804"
     CDS             7106..7816
                     /locus_tag="VYO97_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1754560.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2686 a         1114 c         1576 g         2627 t
ORIGIN      
        1 atatgtgaaa aatagagtac tagcgttaat aggtgaggag ggaattgatc gcatttccga
       61 tgaaaatgac ctgaaacaaa taaaagatta cttagtggaa atcgccttaa aaaacggtaa
      121 aattaaagat ttaatcgagg aaaaggaatg tttaggagca gagttaatga atttcatagt
      181 tcctttacct agtcgattaa atgatatttt ttggagttca tatgatatat cacctcaaga
      241 agcagttgaa gagttttata agttaagtaa agatagtgat tatattaaaa cttctgctat
      301 tgctaagaat attgagtttc gagcatcaac taaatacgga gagctagaga ttacgataaa
      361 tttgtcaaaa cccgaaaaag atccaaaaac aattgctgct gaaaaattag taaaagcaac
      421 taattatccc aaatgtttac tatgtatgga aaatgaagga tatcaggggc gaataaatta
      481 tccagcacgt tcgaatcata gaattattcg gttgaaactt ggtgacgaag tatggggatt
      541 ccagtattct ccctactcgt actttaataa gcatgcgata tttttgaata gtcaacatgt
      601 accaatggct attacctcta aaacatttga acaattattg gagattgttg atattttgcc
      661 aggatatttt gctggttcaa attctgatct tcctatctca ggaggctcca ttcttagtca
      721 taaccactac cagggaggga aacatatttt cccaatggaa aaagcaaaat ttgagagtga
      781 attttgtttt aaagactttg aggatgttaa tgctggtatt gtaaaatggc caatgtcagt
      841 aattaggtta caaagtgaaa ataaaaatcg cttgttagat ttagctacta aaattctaaa
      901 taagtggaga gaatattcag atttagaagt agacatcatt gctatgactg aagatgtccc
      961 acaccatacg gtgactccta ttgctcgtaa agtggacggg agatacgaat tagatattgt
     1021 tctaagagat aatcatacta ctgaacaata cccagatggt gttttccatc ctcatcaaga
     1081 tgtacaacat attaaaaagg aaaatatcgg gttaatcgaa gtgatgggac ttgcgatatt
     1141 accacctcgt ttgaaaccag agttagaaga agttggaaaa tatcttttag gagaggataa
     1201 tgcaattgcg gattatcatt tagaatgggc cgaccaattg aaagaaaaat atcctcgcat
     1261 taataaagaa gaggttaata gcgtagttca acacgaagca ggacaagtgt ttgcgagagt
     1321 tctggaagat gcaggagttt ataagaatac accgtctggg catgaagcgt ttatgagatt
     1381 tgttaagtcg gttggaatta attaggagga aacccagatg gcaatattgg taacaggcgg
     1441 agctggttat attggtagcc ataccgtagt agaattacta aatttaggaa aggaagttat
     1501 cattgtcgac aacctttcga actctagcaa attggtgcta gaccgtattg aagcaattac
     1561 aggaatacgt cccgtgtttt acgaattaga tgtttgtgat aaacaagcat tgagaaaggt
     1621 atttgaacaa gaatcgattg atgctgcaat tcattttgca ggttataaag ctgtcggaga
     1681 atccgtgcaa aagcctgtga tgtactacaa aaataatatt atgagtacat tggcacttgt
     1741 tgaagtgatg tcagaattta atgttaaaaa gattgtcttt tcttcaagtg cgacaaatcc
     1801 ttatgggtat acgaaagtga tgcttgagca aattttaaaa gatgttcatg tggcagattc
     1861 agaatggagt attgcgttgc ttcgttattt caatccaatt ggtgctcatg agtctggttt
     1921 gattggggaa gatccctcag gaattcctaa caacttgatg ccttatattg cacaagtagc
     1981 ggtaggtaag ctatcagagc taagtgtgtt cggaaatgat tatgatacgc ttgatggtac
     2041 tggtgtgcgt gattatatcc atgtagtaga tttagcaata ggacatataa aagctttaga
     2101 aaaagtatct gaaaaaacag atgtttatat ctataaccta ggctcgggag aaggcactag
     2161 tgtattacaa cttgtaaata catttgaaag tgttaataag atccctattc cttataaaat
     2221 agttccaaga cgttcaggag acgttgcaac ttattatgca aatgcagaca aagcatataa
     2281 ggaattaaat tggaggacaa caaaatcgat tgaagacatg tgtagagata catggaattg
     2341 gcaatcaaaa aatcccaatg gctataattg atgatactgt ctatatgagt gctgtatttt
     2401 ggaaaaatta cttaatcttt taatgaaaga taaatttaaa aaaaatgttt gacagcgctt
     2461 gacaaaggaa acggtttcat tgtataatgt aaacacaatg acaccgatgt caaatataaa
     2521 aaagaaggtc tttatatgaa aaacttaaca aaaattaagt tcaaagaaaa tggggaattt
     2581 aatcatttcc ctgggaatac agttgtagca aatctttata ctaaacaaga tttgatggaa
     2641 gttgttgata ttattcaatc acgttataga gaattaccat ttatcgataa gtttacttta
     2701 actccaagga attctattca catgactgta attgaattgt tgtgccatga aaatcgtgaa
     2761 acggagtttt ggagcagtaa tcttcctcta gatacacctt tacaggaaat acatgattac
     2821 tttgctaaac aacttgaaat ttttccattg ttggatgaag aaatttatat gcgtataact
     2881 gaaatgggaa aacaaaacat actagttgaa cctgcagatg aagcttctgc aaagagatta
     2941 gaagaaattc gtacttatgt ctcagaaaaa gcaggtgttt gtttccctaa tcatgataga
     3001 tatcaattcc atatttcaat tgggtatctt cggattcctc taaccgaaga ggaggaagaa
     3061 gagtttacta aagtcagagc agaattaact gaaattttat tagagaagat tccaactatt
     3121 actgtaaacc gtattgacta tactgtattt gaagatatga gacaatttgt tccatatcac
     3181 gaaaaattta aataaaataa taaaaaagga gaacttatca tgaaaaaaat gaaagtttgg
     3241 tctactgtac ttgcaacggg agttgctctt actacacttg ctgcttgctc tggaggttca
     3301 aattctacga ctgcttcttc atctgaagaa aaagctgata aaagtcaaga attagtaatc
     3361 tattcgaact cagtctcaaa tggtcgtggt gattggttga ctgctaaagc aaaagaagct
     3421 ggttttaata taaaaatggt tgatattgct ggcgctcaat tagcagaccg tgttattgct
     3481 gagaagaata atgcagttgc agatatggta tttggaattg gtgctgttga ctcaaataaa
     3541 attagagatc aaaaattact agtacagtac aagcctaaat ggttagataa aattgatcaa
     3601 tctttatcag ataaagataa ttattataat cctgtgattg ttcaaccatt agttttaatt
     3661 ggggcgcctg atgtaaaaga aatgcctaaa gattggactg aattaggtag taagtataaa
     3721 ggtaaatatt caatttatgg tcttcaaggt ggtacaggac gggcaattct agcaagtatc
     3781 ttagttcgtt accttgatga aaaaggagat ttaggcgtat cagaaaaagg atgggaagtt
     3841 gcaaaagaat actttgcaaa tgcctatact cttcaaaaag gtgaaagctc agttgttaaa
     3901 gcgttagata aagaaagccc aattcaatac ggtatgatct ggggttctgg agcgttaatc
     3961 ggacaaaaag aacaaaatgt tgaattcaaa gttatgactc ctgagattgg tgtaccattt
     4021 gtaactgaac aaactatggt tttaagcact agtaaaaaac aagcgttagc taaagaattt
     4081 attgattggt ttggtcaatc agaaattcaa gtagaatata gtaaaaaatt tggctctatc
     4141 cctgccaata aagatgccct aaaagagtta cctgaagata cgaaaaaatt tgttgatcaa
     4201 gtgaaaccac aaaatattga ctgggaagct gttggaaagc atttggatga atgggtagaa
     4261 aaagctgaat tagaatacgt aaaataagaa ataagagtta ttttttatgt ccaagtctct
     4321 ctattatttc taaatttaag gaagaaataa agagaggctt ttggtttagt aatagaggga
     4381 gttagtgaga tgattaaatt tgataatatt caaattaaat atggtgattt tgttgcaatt
     4441 gataatctga atttagatat acatgaaggg gaattcttta catttcttgg gccttcagga
     4501 tgtggtaaat caactacttt gagagcattg gtaggttttt tagatccatc atcaggaagt
     4561 attgaagtta atggaacaga tgtcactcat ttggaacctg aaaagcgtgg aattggtatt
     4621 gtatttcaat cttatgcgct atttccaact atgactgttt ttgataatat tgcatttggt
     4681 ttaaaagtta agaaggtagc tccagatgtt attaaagcta aagtatcagc agtggcagca
     4741 aaaattaaga tctctgatca acagttacag cgtaatgtat cagaattatc tgggggtcaa
     4801 caacaaaggg tagcattggc tcgtgctctg gttcttgaac ctaaaattct ttgtctagat
     4861 gaaccattgt caaaccttga cgcaaaatta cgtgtagatt tgagaaaaga gttgaaaaga
     4921 cttcaaaaag agttaggtat tactacttta tatgttactc atgatcaaga ggaagccttg
     4981 actttatctg atagaattgc agtctttaac aatggataca tcgaacaggt cggtacacca
     5041 gtagagattt atcataattc tcaaactgaa tttgtatgtg attttattgg agatattaat
     5101 gttttgaccg atgaaacagt ccacgaagta ttattgaaaa atacaagcgt tttcttagag
     5161 gataaaaaag gatacattcg attagagaaa gttcgattca atcgtgaaac tgaacaagat
     5221 tttattctaa aagggacaat tattgatgtt gagttttctg gagttacaat tcactataca
     5281 ataaaagttt ctgaaagtca gattcttaat gtaacaagta ttgatagtca ggctgctatt
     5341 agatctgtcg gagaaagtgt ggaattattt atcacaccat cagacgttct gcaattttaa
     5401 ggaggtgcag tatgcgtcat aaattaaatt taaaagattg gcttattcgt ttagggttaa
     5461 tctggttctt agtaacattt attatttatc caaactttga tctagtagtg aatgtatttg
     5521 taaaaggagg agaattttcc cttgatgctg tacatcgtgt tctaaaatct cagagggcac
     5581 ttcagagtat tatgaacagt tttaagttag cattttcact cattattaca gttaatgtcg
     5641 taggtattct ttgtgttcta tttacagagt actttgatat taaaggtgct aaaattttaa
     5701 aattaggtta tatgacctct ttaatttatg gaggagtggt tttagcgact ggatataaat
     5761 ttgtctatgg tccttatgga ttgattacaa aatttttaca aaatgttatc ccttctttag
     5821 actctaactg gtttattggg tatggtgcag tcttattcat tatgacattt tcaggaactg
     5881 ctaatcatac attgttttta acaaatacaa ttcgaagcgt tgactatcac actattgagg
     5941 ctgctcgaaa tatgggagca aaaccattta ctgttttccg aaaagtagtg ttaccaacct
     6001 taattccaac tctatttgca cttactatta tggtttttct tagtggttta tctgcagtag
     6061 cagcacccat gattgttggt ggtaaagaat ttcaaactat aaatccaatg attattacat
     6121 ttgcagggat ggggaattct cgtgatttag ctgccttact tgcaattatt ttaggtattg
     6181 caactacaat tttgcttact atcatgaata agatagaaaa aggtggaaat tatatttcta
     6241 tctctaagac taaagcgcct cttaaaaaac aaaaaattgc gtctaagcct tggaatatca
     6301 ttgctcacat tgtagcatat ggattgttca cagttttcat gcttccacta atttttatag
     6361 tattatactc atttacagat ccagttgcaa ttcaaacagg taacttaaca ttatcaaact
     6421 ttactttaga aaattatcgc ttattcttta gtaatagtgc ggcattctct ccattcttgg
     6481 tcagctttat ttattctatt attgctgcga caacagcaac aattctcgca gttgtatttg
     6541 ctcgtgttgt cagaaaacat aaatctcgtt ttgatttctt atttgaatat ggtgctctac
     6601 ttccttggtt actaccaagt acacttttag cagtaagttt attatttact tttaatcagc
     6661 cacaatttct tgtcttgaat cagattttgg taggtagttt ggtaattcta cttattgcat
     6721 atatagttgt aaaaatccca ttttcttata gaatggtacg tgctatttta tttagtgttg
     6781 atgatgagat ggaagatgca gcaagaagta tgggtgcttc acctttttat actatgatga
     6841 aggttatcat tccatttatt ttaccggttg ttctctctgt tattgcttta aactttaact
     6901 ctttattaac tgacttcgac ttatctgtat tcctttacca tcccctagct caaccattag
     6961 gtattacgat tcgatctgca ggtgatgaaa cagcaacatc taatgcacaa gctctggtat
     7021 ttgtttatac aattgttctg atgattattt ctggaacggt attatacttc acacaaagac
     7081 cggggcgtaa agtaaggaaa taatcatgac agccactagt cttgggttat caaatattga
     7141 aatagttgtc aggattgttt tatcagtagt cattggtagt ataattggtt tagagagagg
     7201 gagcaaatcc cagcctgcag gcatccgaac ttatagtatt gtttgtctag ctgcatgttt
     7261 gattatgatg acgaatgaat acgtatctta taaatttggg acaggagatc ctacacgatt
     7321 aggagctcaa gttatatcag gtgtgggttt tctaggcgct ggaacgattc ttattacaga
     7381 taaaaagaaa attacaggtc tgacaactgc agcaggcatt tgggcttcgg caggaattgg
     7441 attagctatt ggagtaggtt tttatgaggg agctctttta gtagctattt ctgtttgggg
     7501 tgtgatatcc atgttccaac cactaaaaaa atatctgcaa aatcgttcta aaatgattga
     7561 attgtatata gtggttaaat ctacagaagc ctataatcgt gtattggttt attgtgcaga
     7621 aaatggtatc agaatgaccg attcaagaac agcgtttgga gatgttaatt ctgacagaat
     7681 tgaatacttt gatgttccag ataaaaaaat agcgtcattt attactctgg aattatcagg
     7741 tagatttgaa catcttcgac tgatggaaga aattgctaat attgtaggtg ttatttatgt
     7801 cgaagaaatt agctgaaatt tagtaaacaa agggacagat gtttttgtcc ttttgttttt
     7861 tagatgtttt aatcatctaa tttttgagta gatttttgct cagaggtttt tatgaaagat
     7921 tataaaatca attttgattt aggtaaaata gaatattttg ataataattg tttgatacaa
     7981 gtatacaaat ttatttcttt tta
//
LOCUS       DAVMAF010000083         7990 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMAF010000083 DAVMAF010000000
VERSION     DAVMAF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.084"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            35..595
                     /locus_tag="VYO97_001805"
     CDS             35..595
                     /locus_tag="VYO97_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV1754561.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQVLHFVPNRNITVVSNFSRTD"
     gene            652..1560
                     /locus_tag="VYO97_001806"
     CDS             652..1560
                     /locus_tag="VYO97_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1754562.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNFFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1512..2102
                     /locus_tag="VYO97_001807"
     CDS             1512..2102
                     /locus_tag="VYO97_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1754563.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2105..3211
                     /gene="yqeH"
                     /locus_tag="VYO97_001808"
     CDS             2105..3211
                     /gene="yqeH"
                     /locus_tag="VYO97_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1754564.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            3425..3736
                     /gene="yhbY"
                     /locus_tag="VYO97_001809"
     CDS             3425..3736
                     /gene="yhbY"
                     /locus_tag="VYO97_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1754565.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3788..4417
                     /locus_tag="VYO97_001810"
     CDS             3788..4417
                     /locus_tag="VYO97_001810"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1754566.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4417..5010
                     /gene="yqeK"
                     /locus_tag="VYO97_001811"
     CDS             4417..5010
                     /gene="yqeK"
                     /locus_tag="VYO97_001811"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1754567.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            5011..5511
                     /locus_tag="VYO97_001812"
     CDS             5011..5511
                     /locus_tag="VYO97_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1754568.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5524..5877
                     /gene="rsfS"
                     /locus_tag="VYO97_001813"
     CDS             5524..5877
                     /gene="rsfS"
                     /locus_tag="VYO97_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1754569.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            5980..6726
                     /locus_tag="VYO97_001814"
     CDS             5980..6726
                     /locus_tag="VYO97_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1754570.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6736..7833
                     /locus_tag="VYO97_001815"
     CDS             6736..7833
                     /locus_tag="VYO97_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1754571.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         2401 a         1574 c         1828 g         2187 t
ORIGIN      
        1 gaaaatatgg tataatgaaa taaaggagtt ttatatgcaa aaatttattc aggcttatat
       61 tgaaaagcta gatgtgacaa ccattatcga gaatattcta accaaggtca tttctctttt
      121 actgcttttg attgtatttt atattgctaa aaaaatgctt cataccatgg tgcagagaat
      181 tgtcaaacct tctctaaaaa tgtctcgtca tgatgtcgga cgccaaaaaa ccatctcacg
      241 tttactagaa aatgtgttta attatacgct atatttcttt ttactctact gcattttgtc
      301 gattttaggt ttgccagttt ctagtttgct ggctggagct ggtattgctg gggtagcgat
      361 tggtatggga gcccaaggct ttctgtctga tgtcatcaat ggctttttca tcctctttga
      421 acgtcaactg gatgtgggag atgaggtcgt tctgacaaat ggaccgatta ctgtatcggg
      481 taaggttgtc agtgtgggaa ttcgtacgac acagcttcgt agcgaggagc aagtccttca
      541 ctttgtccct aaccgaaata tcacagttgt tagcaatttc tcacgcacag actagacctg
      601 ttattttaag taatttgtgg tacaatagag ggagtttaat aaggagaaaa gatggtttta
      661 gaaaagcagt tgggcaatgg ttgtacctgg atagacctag acctaggaaa gttgaataaa
      721 ctagaagacc tttctgaaat ttacggtttg gacaaggaaa ccattgaata cgcactggat
      781 agaaacgagc gcgcccacat ggactaccac cgtgaaagtg ggacggttac ctttatctat
      841 aatgtcctag acgtaaaaaa ggacaaggcc tactatgaga cttttcccat gacctttatt
      901 gtcgagcatc gtcgcctgat taccattagt aataccaaga acgcctatgt cattgaacag
      961 atgactcgtt atctggagaa ccatgacacg ctttcgattt ataagtttct ctttgccagt
     1021 ctggaaatca tcagcaatgc ctactatcct gtcattgagc agatggacaa gagtagggat
     1081 gaggtcaatg acctcttgcg ccagcgaact accaagaaaa acttctttgc cctgtctgat
     1141 ttggagactg gtatggttta tctgacggca gctgccaaac aaaatcggat tttgttagag
     1201 catattcaag gtcatgcctt gtatcgtagt tttgatgaga ttgagagaga acagtttgat
     1261 gatgccatga ttgaggctca tcagctggta tccatgacag acctaatctc tcagatttta
     1321 cagcagcttt cagcctctta caacaatatt ctaaacaata atctgaatga caatttgaca
     1381 accttgacta tcatttcagt cttgctagct gttttggcag tcgtgacagg ctttttcgga
     1441 atgaatgttc ccttaccttt aacagatgag ccccatgctt ggctctatat cagtttggct
     1501 agtgcaggtt tgtggattgt tttatccttg ttactaagga aaattgcgaa aaaaagttaa
     1561 gaaaaggagc cagaatggcg attgaaaatt atataccaga ttttgctgtg gaagcagtct
     1621 atgatctgac agtcccaagc ctgcaggcgc agggaatcaa ggctgttttg gtcgatttgg
     1681 ataataccct cattgcttgg aacaaccctg atggaacgcc agagatgaag caatggctac
     1741 atgaccttcg ggacgcgggt attggcatta tcgtagtgtc aaataacacc aaaaaacgcg
     1801 ttcaacgagc agttgagaaa tttgggattg attacgttta ctgggccttg aagcccttca
     1861 catttggtat tgaccgtgct atgaaggaat tccactatga caaaaaggaa gtggtcatgg
     1921 ttggtgacca gctcatgaca gatatacgag cagcccaccg tgcagggatt cggtcaattt
     1981 tagtcaaacc cttggtccaa catgactcaa tcaaaacgca gattaaccga actcgtgagc
     2041 gtcgtgttat gagaaaaatc actgaaaagt acggaccgat tacatataaa aaaggaattt
     2101 aactatggaa gaaattctct gtattggttg tggagcaacc attcagacga cagataaagc
     2161 tggtcttggt tttacccccc agtcggcact tgaaaaaggt ttggagactg gcgaagtcta
     2221 ttgccaacgc tgtttccgtc tccgccacta caatgaaatc acagatgtcc agttgacgaa
     2281 cgatgatttc ctcaagctct tgcacgaggt gggagacagt gatgctttag tggtcaatgt
     2341 cattgatatc tttgatttta atggatctgt catcccaggt ttaccacgtt tcgtctcggg
     2401 caatgatgtc ctcttggtag gaaataaaaa agatatcctt cctaagtcag ttaagtctgg
     2461 taagattagc cagtggctca tgaaacgtgc ccatgaagaa ggtcttcgtc cagtcgatgt
     2521 ggtcctaact tcagcacaaa ataaacatgc cattaaggaa gtcattgaca agattgaaca
     2581 ctaccgtaag ggccgcgatg tctatgtggt cggtgtgacc aacgttggaa aatcaactct
     2641 aatcaatgct attatccaag aaatcacggg tgatcagaat gtcatcacta cttcacgctt
     2701 cccagggaca accttggaca aaatagagat tccgcttgac gacggatctt atatttacga
     2761 tacgccggga attatccacc gccaccagat ggctcactac ttgacggcca aaaacctcaa
     2821 gtatgtcagt cctaaaaagg aaatcaagcc taagacctat cagcttaatc ctgagcaaac
     2881 cctattttta ggtggtttgg gacgctttga ctttatagca ggagaaaagc aaggatttac
     2941 tgctttcttt gataatgaac tcaaactcca tcgtagcaag cttgaaggag ctagtgcttt
     3001 ctacgataag cacctgggaa ctcttttgac accaccaaat agcaaggaaa aagaagattt
     3061 cccaaggcta gtccagcatg tctttaccat taaagataag acagacctag tcatctcagg
     3121 cctaggatgg attcgtgtaa caggcacagc aaaagtcgcc gtctgggcac cagaaggcgt
     3181 cgccgtcgtc acacgaaaag caattattta aaacaaaatc gctgagcgaa caaagtgagc
     3241 tcatatagcg atagttattg ccgtggtgtg attgccaatc tgtagattgg caaggggcac
     3301 aattgagacc aaggctcaat tgtgaggtca ggaaatccat ttttcaaaga tgaaatcttt
     3361 ggaaaattag ttccgaccgt ccctcaccac ctaaaataac tatcaaaaaa caaggaagaa
     3421 aattatgtca ttaacatcaa aacaacgtgc cttcctcaac agccaggcac acaccctcaa
     3481 acctatcatc caaatcggga aaaatggact caacgaccaa atcaaaacca gcgtccgtca
     3541 agctcttgat gcgcgtgaat taatcaaggt tactctctta caaaacacag atgaaaacat
     3601 ccacgaagta gctgaaattt tggaagaaga aatcggtgtg gatacagtcc aaaaaatagg
     3661 acgcatcttg attttgttta aacaatctag caagaaagaa aatcgcaaga tttctaagaa
     3721 agtcaaagaa atctaagacc gaaactccaa ataactgttt ttatagagaa ataaagggga
     3781 ctagcctatg acaatcgaac tattgactcc ctttaccaag gtagagttgg agccagaaat
     3841 caaggagaaa aaacgcaaac aagttgggat tttagggggg aattttaacc ctgttcacaa
     3901 tgcccatctc attgttgcgg atcaagtacg gcaacagttg ggactggatc aagttctgct
     3961 catgcctgaa taccaacctc ctcacgttga taaaaaggaa accatccctg aacaccatcg
     4021 tctcaagatg cttgagttgg caattgaggg aattgacggc ctagtcattg aaaccattga
     4081 gttggagcgc aagggtattt cctacaccta cgacaccatg aagattttga cagagaagaa
     4141 tccagatacg gattattact ttatcatcgg tgccgacatg gttgactatc tgcctaagtg
     4201 gtaccgaatt gatgaactgg ttgacatggt tcagtttgtg ggggttcagc gtccacgcta
     4261 caaggtaggg acttcctatc cagttatctg ggtggacgta ccgctcatgg atatctcgtc
     4321 cagcatggtg cgtgccttcc ttgcccaagg tcggaaaccc aactttctcc tacctcagcc
     4381 agtgctagac tacatcgaga aggaggggct ctactgatgt cctatcaaga ctatatcaac
     4441 tgctcccgtg aggctttgtt ggaaaaaatg gcagagcttc tacctgagaa acgtctaacc
     4501 cattgtctag gtgtggagcg tgccgctatg gaattagctc agcgctttgg tgtagatgtt
     4561 gagaaagcaa gtctagcagg tcttcttcat gactatgcta aaaagttgtc agatcaggaa
     4621 tttctagact tgattgaccg ttaccagcta gaccctgacc tcaaaaactg gggcaataat
     4681 gtctggcatg gtatggtcgg aatttacaaa attcaggaag atttggattt gcatgattca
     4741 gaaatcctgc gagccattga agtccataca gtcggtgctg gtcaaatgac agacctagat
     4801 aaggtcatct acgtcgcaga ctatatcgag cacaatcgag cctttcctgg agtagatgtg
     4861 gcgcgtgaga ttgcaagtct atcgctcaat aaggcggtgg cctacgaaac agctcgtacc
     4921 gtggagtatc tagctcatca gggattcccc atctatcccc aaacccttga aacctataac
     4981 gcctttgtgc actatttgaa agaggactaa atgaagacgg cttttataat cattgatgtt
     5041 cagaatgttt tagtagaaac tgggttccag acaaaaagtc tattggaaaa aatttcttat
     5101 ttacaaaacc aggctagaag caagaatatt gaaattatct atgttcaaca tattgagaac
     5161 tctgaagctc aaacatcaga agattggcag ttatctgcgc ttttaaatcg aaaacctgct
     5221 gaaaaggtct ttcagaagaa gtataacagt attttcaaag aaactggctt aaaagaatac
     5281 ttggataaac aggggattga aaaattagtt ttatgtggta tgcagacaga atattgtgtg
     5341 gatacctctg tcaaggttgc ctttgaatat ggctatcagc ttattgttcc agaaggtgct
     5401 gtcacaacct ttgatgggga tgacattcca gcagaaacga ttaatgaatt ttatgaggac
     5461 atttgggagg agcgctttgc agatgtccta gattacaaac atattttcta aaaagaggat
     5521 taaatgaacg aaaaagaatt actagaacta gtcgtgaaag cggctgatga gaaacgtgcg
     5581 gaggatatcc tcgcacttga tgtacaagat ttgactagtg tgacggacta ctttgtcatc
     5641 actagctcaa tgaatagccg tcagttggac gctatcgcag ctaatatccg tgaaaaagta
     5701 gctcaagcag gctttaaagg tagccatgtc gaaggcgatg cagctggagg ctgggtctta
     5761 ctggacctcg gtgctgttgt cgtgcatatc ttttcagaag aaatgcgtgc ccattataat
     5821 ctagagaagc tatggcatga ggcgaattca gtagatattt cagaagctct tgcttagaag
     5881 atgaactcag ttgtagtcaa ctgggtttct ttgcttattt tagaaaaatt gatagaaagg
     5941 taaagggcta gtggtgttta ccatggagct cttagtatca tgattatggc gacttatgaa
     6001 acctttgcgg cggtctatga tgcggtcatg gacgatagtt tatacgacaa atggacgaat
     6061 ttttctctgc gtcatttgcc taagaccaag gagagaaaga aactcttgga attggcttgt
     6121 gggacaggaa ttcaatcagt gcgcttctct caggctggtt ttgatgtgac tggacttgac
     6181 ttgagtgcgg atatgttgaa gattgcggag aagagagcaa cttcagccaa gcaaaagatt
     6241 gcttttatag aaggtaatat gctgaatttg tccaaggcag ggaaatacga ttttgtcacg
     6301 tgttattcgg actctatctg ctatatgcag gatgaggtgg aagtagggga cgtctttaag
     6361 gacgtgtaca atgcgctgaa tgaagaggga gttttcatct ttgacgtgca ctcgacctac
     6421 cagacagatg aagtattccc tggctattcc taccatgaaa atgcggaaga ttttgccatg
     6481 ctttgggata catatgagga cgaagctcct cactccatcg tgcatgagct gactttcttt
     6541 atcaaggagg ctgacggttc ctttagtcgc cacgatgaag tacatgagga gaggacctat
     6601 gagatcttga cctatgatat cttgctggaa caggctggat ttaagtcctt caaactctat
     6661 gcagactttg aggataaaga gccaacagaa actagcaccc gttggttttt tgtggcgcag
     6721 aagtaggaga ttagcatgac cataacaggt attatcgcgg agttcaatcc ttttcataat
     6781 ggccataaat acctgctgga tcaggcagag ggactgaaaa ttgttgccat gtcagggaat
     6841 ttcatgcagc gtggagagcc tgctattgtg gataagtgga caaggaccca gatggcgctg
     6901 gaaaatggcg cagacttggt agtggaattg ccctttttag tcagtgttca ggcagctgat
     6961 ttctttggtc aaggagctat ggatatcttg gatcgcttgg gcattgatag tctagttttt
     7021 gggacagagg aagttcgaga ttatcagaaa attgctgact tatacacaga gaaaggtgct
     7081 gagatggaga aatttgtgga aaatctgcct gattcccttt cctatcccca gaaaacccaa
     7141 gctatgtgga aggaatttgc tggtcttgat ttttcaggta atacccccaa tcatgttctt
     7201 gctctggcct atgccaaggc ggttgcagga cgaaatatca agctgcatcc gattcagcgt
     7261 cagggggcag gttaccattc tgtgaataaa gatgtggact ttgcctcggc gacagccctc
     7321 cgtcaacatc agaaggacca agatttctta gaacgtttta tgccttctgt tgccctattt
     7381 gagcaggcca gtaaggtgat ctgggaagac tattttccct tgctccgcta tcaaatcttg
     7441 tcaaatcccg acctaaccac catctatcag gtcaatcaag aaatggctgt gcgcattaaa
     7501 gaagctatta aaacagccca gtctgtggaa gaattggtcg agttagtgac taccaaacgt
     7561 tacaccaagg cgcgtgtcag acgcctattg acctatattt tggtgcaggc tagagaaaat
     7621 gtcttgccag aagccattca tgtccttgga tttactgaaa aaggcaggca acatctcaag
     7681 tctctgaaag gacaggtcaa tctagtcagc cgaattggca aagaaccttg ggatgctatg
     7741 actcaaaagg ttgaccagat ttaccaacta ggaaagccaa gtatagcaga gcaaaatttt
     7801 ggaagagtgc cgattagaat agaaacaaac taagtctacc gaaaggtagg cttttttatt
     7861 gtagaaattt tagataacaa attgaagata aagtttttgg aaaatgataa actccctaat
     7921 acagaagaca aaaagtcaat aaagaaaatt agtatatgaa ccttaatctt tatctttgaa
     7981 tccgctttat
//
LOCUS       DAVMAF010000084         7306 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMAF010000084 DAVMAF010000000
VERSION     DAVMAF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.085"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            451..677
                     /locus_tag="VYO97_001816"
                     /pseudo
     CDS             451..677
                     /locus_tag="VYO97_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            842..1090
                     /locus_tag="VYO97_001817"
     CDS             842..1090
                     /locus_tag="VYO97_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1754572.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRLNLDK"
     gene            complement(1411..2916)
                     /gene="pyk"
                     /locus_tag="VYO97_001818"
     CDS             complement(1411..2916)
                     /gene="pyk"
                     /locus_tag="VYO97_001818"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1754573.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTSEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(2975..3982)
                     /gene="pfkA"
                     /locus_tag="VYO97_001819"
     CDS             complement(2975..3982)
                     /gene="pfkA"
                     /locus_tag="VYO97_001819"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1754574.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4065..7166)
                     /locus_tag="VYO97_001820"
     CDS             complement(4065..7166)
                     /locus_tag="VYO97_001820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1754575.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASFLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2220 a         1605 c         1280 g         2201 t
ORIGIN      
        1 aaacaatata gactagtaat ggttggactc aataagaatt gggtactaag ataggtataa
       61 gtaaaaacac cattagaaat tatgaaaaaa gggttaggtc tactaagaaa aatactatat
      121 ttgacttagt taaagtattc agtagcttga ttgatgccct ctttcctcca gttcaaaagg
      181 attctcctag cgatatccaa tctatctacg accaacgggc accacccaga caaggtaaag
      241 ttctgaccta tgccgagagg caactgtatg atcagaaaaa cgaagtatcg taaaaatcat
      301 cagactggac gactacagac agactactta ctctcgtgtt aggtagttta tctctgcagg
      361 tagccgctcg atgcaggacg acgatttagt tatggagttt ccattatccc agacgactac
      421 gacccaatcg cttatgtcgt cagcaagtcc atgaagccaa agataagaaa cagcgactac
      481 ctatttatca agaatacccc acagattgac tataatacca tcggcatctt ccaagtagac
      541 ggcactaact atgtcaagaa actcccgcca aggctgcctc aaaaaaactc aatcccgaat
      601 accccgacat ccacctagac gaaagcaacc atattcgtac catcgggaaa gctatcaggc
      661 tatataggga gaattaaaaa agggggagaa tggcgatata aaacaaacag attttaaaag
      721 attcgaaaaa ctttgaaaaa gttcattcta catttcaaaa aagaataagt gacctaaagt
      781 agattagatt ttgttaaaca atagtgatat gtaaatttag agaaaggcat ttaagatcac
      841 tttgcaaaag gcaaatacta aactagagaa accgataaac cagcccctta gttcccatat
      901 tttcaggcac acactcctta gtacactagc tgagaagaat gctataatgg ttagagtgga
      961 acacaaaaac gctaaaacag taaataacat ctacactcac gtaagtaaaa gaatggagca
     1021 agctactttg gaggttttaa ataccatatc attaaatcga aaatatatca gattgaatct
     1081 agacaagtag attgcgattg ctaaaacatt tgtagaaatt catttgactt tctaatcaat
     1141 ttgtgcatat cttatttcat actcaatgaa aatcaaagag caaactagga agctagccgc
     1201 aggttgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gtaacatata
     1261 tacggtaagg cgacgctgac gtggtttgaa tttgattttc gaagagtatc aatccattat
     1321 gaaagttttg cccccttttt gccccctgga caaaaaaaga gcccatcata caggctagaa
     1381 agctggatat gataggtttt tatatttttc ttaacgtact gtgcggatac gcattgtgtt
     1441 tgtgcgaaca gcttctccta ctggcacacc agcaacgata acgatatcat cgcctgactc
     1501 aacgagacct gcttctaccg ctttacgttc agcgatttcg aacatatcgt cagttgaaga
     1561 tggagcatct gtcaacattg ggataacacc ccagttcaac atcaagccac gttctgtcaa
     1621 ttcgtcaaat gtcaatgcta agatgtcagc atttggacgg tatttagaaa tcaaacgtgc
     1681 agtatgacct gtcttagtaa gagttacaac caatttgata tccattgagc tagtagcatc
     1741 tttaacagca gaagccatta cttctgtctt agagttacgc tcaaatgaat ctgaatcaag
     1801 acgtccgtat tcattaagaa gagcttgagc gttcttgtcg attgtagcca ttgtagttac
     1861 tgactcgagt gggtatttac cgtttgcaga ctcgcctgac agcattgtag cgtcagttcc
     1921 gtcgataaca gcgttgaata cgtctgatac ttctgaacga gttgcacgtg gtttttcagt
     1981 cattgtttca agcatgtttg ttgcagtgat aacaacttta cctgcagcat tgactttctt
     2041 gataatcatt ttttgataaa ctggaaccat ttcgaacggt acttcgatac ccatatcacc
     2101 acgagcaatc ataataccat cagctgcttc gatgatttcg tctaagttat cgataccttg
     2161 ttggttttcg attttagcga acaattgaac atgtccgttt ccagtttctt cacagattgc
     2221 acgaacttcg ttcacatctt ttgcagtacg tacgaatgaa attgcgatga agttgatacc
     2281 ttgttcaaga ccgaaacgga tatcgtcgtt atcgcgttca gcaagagctg ggaaaggaat
     2341 tttagtgtta gggatgttca cacctttttg tttagcgatg ataccatcgt tttcaacttc
     2401 aacttcaaat tcacgagttg catcatcttt agcaaccaca cgaagaccaa gtttaccatc
     2461 gtcaaccaaa acttgacgac caacttcaac atcatcatag atatcaagag caccagcaac
     2521 gttcaacgca atcacttcac gagttgattt gattccttgt ttagttgcaa cacgaatttt
     2581 ttcactagtt ttgtatgaat attctttagc ttcaccttcg aacaattctg tacggatttc
     2641 aggtcctttt gtatcaagaa ggaaaccaac ttttttacct gcaatttttt ccgcaagttt
     2701 aacagttgcc atacgctcac cttgttcttg gtggtcgccg tgtgagaagt tgaatcggaa
     2761 tgtgttagca ccagcttcaa tcaatttagc aatgttttta gctgaagctt caacatcaag
     2821 tttttcaccc cagtatccat cctcaccgaa ttttttacca ccacggattt ctaccgcagg
     2881 acccaaagtt gcaacgatct ttacacgttt gttcatgatt tttgtgactc ctttatatat
     2941 atgacctttt tggtcttatt aactaaattg taaattatga caagctctta ttcaagctag
     3001 atagctcaat atcggctttg tgcgggttgt taaccacaat cttaccttct gcagtaaggc
     3061 taaacaatgc cccttcttct gcagtaccaa gaattggatt ttcaaccatt ttttcgttac
     3121 gaataccaac cgcaacacca ccgatacctt ctttaagaag tttaacagca tgtgcaccca
     3181 tacgtgacgc caaaacacgg tcacgcgcag ttggagaacc accacgttga atatgtccaa
     3241 gttctgttac acgaaggtcg cttgtatctc cagcttcttt aagtttttga ccaaattcag
     3301 ccgctgacat cacaccttca gctaagacga tgatattgtg ttttttacca cattcataac
     3361 cagctttgat gcttgctacg atatcttcca tcttgaagcc tgcttcaggg atgatgattt
     3421 catcagcacc agttgcaata ccagcccaaa gagcgatatc accagcgtta cgtcccataa
     3481 cttcgattac aaaagtacga cggtgacttg atgatgtatc acgaatctta tcgatagcgt
     3541 ccatggcagt agtaaccgct gtgtcaaaac cgattgtaaa gtcagtacca acgatatcgt
     3601 tatcgattgt acctggaaga ccaatagctg ggaagccatg ttcagtcaaa cgcatagcgc
     3661 cgtggtaaga tccgtcacca ccgataacaa ctacaccttc aattccgtgt tttttcaatt
     3721 gctcaatccc tttaagttgc ccttcaagtt gagcgaactc tggataacga gctgagtgaa
     3781 gaaaagtacc accacgagaa atgatgtccc ctactgaagc tgcatccagg ggatgaattt
     3841 caccagcaac cataccagca tatccgtcat agataccaaa cacttccatt ccttctgaaa
     3901 ttgcttgacg aacaactgca cggatggcag cgttcatacc aggtgcgtct ccgccactag
     3961 tcaaaacagc aatacgtttc atattggttt atgctccttt ttcttttaac attcttcttg
     4021 attatatcac atttgatttt aaaattcttc tattttccgt atttttagcg ataaatcgtt
     4081 ttcataacga tttcattcaa tttctcctct aattcattgg atttagctac aaaatgatgg
     4141 ggagaaacga tggttttctg ttcctcttca taccggatga tgactgggat tgggccttta
     4201 aattgttcta aaatgcgtga aatttcttga tccgattcat gatttttcac ctgtatccaa
     4261 aagcgttcag caactgcttc tcttatttct tgtgcaatca tttgcagacg gccatcacgt
     4321 gattgtattt ttccttttac atagtagaag gctccctctt ttatttcctg tccaacctga
     4381 cgatataagt ctgaaaagag agtgacatcc aattttttct tactatcatc tgcctgtaag
     4441 aaggccatat tttcaccctt tttggtacga atcactttta ttttctgaac ttcaaccaag
     4501 ataatagcat agctattttc tgacaaattt ccgattgggg taatcgggta aatagcctta
     4561 cttgcaatag cttgtagtgg atgtttgctg acacctatcc ctaaaagctc ttgttccata
     4621 taaaattttt cttgttccgt ccaatcttcc gattcctgcc aactataaat agcatctcca
     4681 aacaaacttc ccaactcttt cacaaattca aatagattag ctaaattatt aaatactttt
     4741 tgacgatttt tttcaaatga atcgaaaaga ccaactttta ccaaaggttc tagcagagga
     4801 agtttcagat aattctcagg taatttagct ataaaatctt caatgttaga ataaggtcta
     4861 ttttcaataa tccagagagc taaatcatta ctgactcctt taatggattt caaacctaga
     4921 tagatggcct tgttggcaat tttatcgtga tagggaatcg tgttgatgga tagagaggct
     4981 acttcaaaac ctgcttcaag tgcatctatt aagtaatcac tgttggaaga atttaacatg
     5041 acctgataaa aaatggctgg ataatgtgtt ttaaaataag ccaactggaa ggccaaggct
     5101 gagtaggcat aggcgtgtga cctgttaaaa ccataacctg caaacttctc cataacatca
     5161 aagacctgct ctgctttttc cacagtatga ccagcttcta atgaaccttg aataaaggaa
     5221 gccctcatct catgcatggc agaggcatcc tttttcccca tagcccgacg caaaatatcg
     5281 gctttcccaa gactaaatcc ggcaaatcgc tgggcaacct gcataacctg ctcctgatag
     5341 agcattatgc cgtaggttgg agccaaaata tcctccagta ctggatccag aacagtcact
     5401 tcttcctgcc catgctttct tgccacaaaa ttattgatat agtcactagc acccggtcga
     5461 tttagagaag tagtcgcgac gacatcttca aaacagactg gttgcacacg cttaagcaga
     5521 cgaatggcac ctggttgctc aaattgaaag ataccttttg tatttccaga ggcaaataaa
     5581 gctaacgttt ctttgtcttc caaatctatt tcttcaattt taaggtgaat accttctatt
     5641 tcagcaagca actcttgcat cttctggaca aaggtcaaat ttcgtagtcc cagaaagtcc
     5701 atcttcaaaa gtccgctagc ctcaactcca tgagcatcat actgagtcag tggaatttca
     5761 tcaccatact ttagaggaat gtagttggtt aaatcttggt cactaattac aacaccagcc
     5821 gcatggacag aggtttgcct tggatagccc tctatcttgc aagcaatctc aaaagctttt
     5881 tggtattcta acttactatt gatttgctga cgaaactgga gattgccctc ataggccgac
     5941 ttaagattgt cacgaaaact gattttctta gtaattgcag ataattcata ctctggcaca
     6001 ccaaagcgtt tcaagacatc tcgaagagct tgcttggctc caaaggttga aaaagtaacg
     6061 atttgtgccg catgtttact accatattta ttaccaacat atctgataaa atctggacga
     6121 taaatatctg ggatatcaat atcaatatca ggcatggtat agcgttcacg attaagaaag
     6181 cgttcaaaaa tcagattttt ctctactggg tcaatccccg tgatgtctaa ggcataagaa
     6241 accaaactgc ctactgcaga accccttccc attcccatat aatagccatt cgatcgtcca
     6301 aaacgcaaca aatcccaaac aaccaagaaa taatcatcaa agcccatatc atgaataaca
     6361 gacaattctt ggtctagtct atcttgatat tctttactag tcaacccctt ctgaacaagc
     6421 cccagttcag cacgctctct caactcctct actgctggtc tagctggatt aaaacgaggc
     6481 agtttcagac tagtatccaa gtcgtaagaa atgcctgaaa taagcttttc taaattgtcc
     6541 aaagcttgcg gaaaacgctc ttggaatagt ttctctaaaa aacttgctga tataaagaca
     6601 tcttgtctcg aacgcaaggg aacttctctg agcggtagat tttctttaat cgctgttaaa
     6661 acttgaagaa cttctctatc cctgctttca aaagcgttga cccgataaag aggtaagata
     6721 ggatgatgaa attcgcttgc tagtgtttct ggataaaccc ctatatagta atcacagcct
     6781 agttctaacg actcaactct atcaaaataa ggcacaatga ccgcgatatc ctccaggtac
     6841 tgggacagga ctgaccaagt tttctccccc tgcatcttgg ctgtcgaaag cttcatcaac
     6901 tgctgatagc ccacactaga tagagctaaa aagcgcaaat tcacttcctg atcatctaca
     6961 aacactgtca tttcaagccc tagcaaagga tgaatgccgt atttttttgt aatctctaga
     7021 aagtcgaaag cgccataaag attgtcaata tccatcatag ccaaatgagt gtagccgtat
     7081 tctttagctg ctctcacata cttttcgatc gaaatgacgc tttccataaa actatagact
     7141 gtttttgtat ctagttgtgc gatcaattta cacttctcct ctatccttct cactatatta
     7201 taccattttc acttataaat ggcttctctt gagaaaaatt tcatcagctt actaatttat
     7261 tttctattca tttgtatttt tgtcatcatt tttatattta tactat
//
LOCUS       DAVMAF010000085         7210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMAF010000085 DAVMAF010000000
VERSION     DAVMAF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.086"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(185..1153)
                     /locus_tag="VYO97_001821"
     CDS             complement(185..1153)
                     /locus_tag="VYO97_001821"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1754576.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1298..2078)
                     /locus_tag="VYO97_001822"
                     /pseudo
     CDS             complement(1298..2078)
                     /locus_tag="VYO97_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2103..2600)
                     /locus_tag="VYO97_001823"
     CDS             complement(2103..2600)
                     /locus_tag="VYO97_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1754577.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2673..4034)
                     /gene="radA"
                     /locus_tag="VYO97_001824"
     CDS             complement(2673..4034)
                     /gene="radA"
                     /locus_tag="VYO97_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1754578.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSININRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4048..4563)
                     /locus_tag="VYO97_001825"
     CDS             complement(4048..4563)
                     /locus_tag="VYO97_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1754579.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4565..5008)
                     /locus_tag="VYO97_001826"
     CDS             complement(4565..5008)
                     /locus_tag="VYO97_001826"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1754580.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5065..5161)
                     /gene="ffs"
                     /locus_tag="VYO97_001827"
     ncRNA           complement(5065..5161)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYO97_001827"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5194..5661)
                     /locus_tag="VYO97_001828"
     CDS             complement(5194..5661)
                     /locus_tag="VYO97_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1754581.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5862..7148)
                     /locus_tag="VYO97_001829"
     CDS             complement(5862..7148)
                     /locus_tag="VYO97_001829"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1754582.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         2229 a         1802 c         1205 g         1974 t
ORIGIN      
        1 cgtcgattca agtgctatca ctgttcaaaa atgatggtcg ctgaaacttc tgatgacgta
       61 cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt actagattca
      121 ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg
      181 gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac
      241 gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta tcaatcaaac
      301 gctcttctgg aagatagatg gtatccaaaa caaccaattt cttaatagct gatttttgga
      361 tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag acttcaacag
      421 caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta tcaatcatat
      481 catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata acttcactag
      541 tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt ttcaaaaatt
      601 ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc acatagtcag
      661 aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc atcaaatgat
      721 ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg gtcaataaac
      781 gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt ggctcacgcg
      841 ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca ttgacagatt
      901 ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga ttgtcattta
      961 caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat tcttcaatgt
     1021 tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac tctatcccaa
     1081 tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac agctttaaat
     1141 cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc acaagatttt
     1201 ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa gaagggcacc
     1261 atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt
     1321 tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta accactggaa
     1381 tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag aagtgagttc
     1441 ccaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt cctttatagg
     1501 ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa
     1561 ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat
     1621 ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa gcaattccaa
     1681 gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca
     1741 ataatttcaa gggttcttct accaaatgta ctatctggga aaagaacccc cagtaaatca
     1801 tggatataag tattagcaaa actagacaac cagcctgaaa ggaacatccc tcccaataaa
     1861 gacagaatca aaaccttctt tggcaattcc cattttttcc caatacggaa gagaaaataa
     1921 agagccggaa tcatgtaaaa gagagctaga aagatagaaa ctcccattag tccatattcc
     1981 gcacctgacc tcgaaccgtc cgtatagtag atggtttcat actgtaaacc aatacatagc
     2041 aataaaataa aaataaataa aatactgctt ttcttcatac actttctttc taaatgaagt
     2101 atttataatt ctacgactgt catacttcct gtatcaacat tgtaaatggc accagagata
     2161 atgacatcgt ctggtattag gggagactcg ataagcagtt gcatatcctc gcgtacactc
     2221 tcttctatat cttggaaggg caagaagtcc tggtctgaca catctacacc taattcctct
     2281 tttaaatact cctgaaaagg ttcattttca aaggtctgag caccacagtc tgtatggtgc
     2341 aataccacaa tctctcttgt ccccatttgt tgctgggaaa taactagcga acgaatcatg
     2401 tcttcagtca ctcgaccacc tgcattccgc aagatatgag catccccaag tgccaagccc
     2461 agagcttgcg caacgtgcag acgagagtcc atacaggtca caatagctac tcttgttttg
     2521 ggtttaagtg gcagatttaa ctgcccatgt agggcaacat aagcctgatt ggcttgcata
     2581 aactgttcaa aatacgacac gattccctcc ttgaaaattt gatagtcaaa tatttctcct
     2641 atcttatcat ttttaagaga atttgtcacg gattatgcaa agaccttttt caagacttcc
     2701 tgaatcgttg tcacgccaat gacctgaatt tccttaggca gagtgattcc tgtcaaggaa
     2761 ttctgaggta cataaatctt agtaaagccc agtttagcag cttcgttgat gcgttgctca
     2821 atacgattca cgcgccgaat ctctcctgtc aagcccagtt ctccgacaaa acattcctga
     2881 ggattagttg gcttgtcttt gtagctcgaa gcaatagcaa ctgcaacagc caagtcaatg
     2941 gcaggttcat ccaatttaac accaccagca gatttgagat aggcatcctg attttgcaag
     3001 agaagccctg cccgtttttc caaaacagcc ataatcaagc tagcacggtt aaaatcaagt
     3061 cctgtcgtag tacgcttggc atttccaaac atggtcggtg ttaccaaagc ctgaacctcc
     3121 gccaaaatcg gacgcgtccc ttccatggtt acaacgatgg aggaaccagt cgccccatcc
     3181 aaacgctctt ctaggaaaac ttgactcgga ttgagtacct caaccaagcc gcccgactgc
     3241 atctcaaaaa tcccaatctc attagtggaa ccaaaacgat ttttgaccgc tctcaaaata
     3301 cgaaaggtgt ggtgacgctc cccttcaaag taaagcaccg tatccaccat atgctccaac
     3361 atacgaggcc cagccaaggt tccttctttg gtcacatgac ctacgataaa gatggcaatg
     3421 ttattggtct tggccaactg catgagttca gcggtcactt cacgtacctg agaaacagac
     3481 ccctgcaccc ctgaaatctc aggagacatg atggtctgga tggaatcaat aatgagaaag
     3541 tctggctgga tacgctccac ttctgcacga acactctgca tattggtctc tgcatagaga
     3601 taaaactcac tatcaatatc acctaagcgc tctgcacgta gtttaatctg ctgggcagac
     3661 tcctccccac tgacatagag aactgtcccc acttgggaca actgggttga gacttgtagg
     3721 agaagagttg atttcccaat cccaggatcc ccaccgatga ggacgagact tcctggtacc
     3781 actccgcctc caagcacacg gttgaattcc tccatctccg tcttggttcg attgatattg
     3841 atggaagtca cctcagctag tttcatgggc ttggttttct cacctgtcaa ggacacacgc
     3901 gcattcttga cctcggcaac ctcaacctct tccacaaaag aagaccaaga cccacagttg
     3961 gggcaacgtc ccagatattt aggggaatta tacccacaat tttgacatac aaatgtcgct
     4021 tttttctttg cgatgacaaa cctctttcta tatctctaac tcacactcaa tcacttggca
     4081 aaaatcaatc ttctcatttg gcacaaactg gcgcatgagc attcgatgag caacaactac
     4141 cacagtctga tgttctcgat acttagacat acattctaga aaccgagact tcatttccgt
     4201 agctgtctca tattgaatag gactattagg aagcaactcc cccttgtttt ctaaaaacag
     4261 tcttctagct gtttcaaagt tttctattcc tgttttatag acctgccatt catgtaataa
     4321 aggctctact cttaaaggaa aacccgtagc acagaccaca tacgaagccg tttctaaagc
     4381 tcttgtgact gcagaagata cgattatttc agctgacgag agtaaaggat ttttgctcaa
     4441 tttctggact tgctgccgtc ccatctcaga caagggtgcc aaatctatcc caaatcctat
     4501 ataagaacgc tcctctaact cacggtaatc tggctcccca tgacgtacaa agataatctt
     4561 cattctagtg ccctgtcgat ccaaatccac cagttcgaac gccatcagcc gcatctccat
     4621 ctgcaattaa gaaagtagca aaaacagcct ggacaatacg ctccccaact tcaagaacaa
     4681 cctcttggtc tgtgatattc ttcatctgcg caaaaatatg cccttcattt ccaggatttc
     4741 cataataatc cccatcaatg accccaactg agttaattaa aaccaagccc ttcttacgag
     4801 gatttgaaga acgatcatag aggtagagaa cctcagtcgg ctgcatataa gccttaaccc
     4861 ctgtcggaac caagacaatc tctcctggcg caacaactgt acgcacagca acctttaagt
     4921 cgtaaccagc cgcatgcgct gtctcacgct tgggcaataa attttcatct gtaaaactcg
     4981 aaaccaattc aaaaccacga attttcataa ttttctcttt tctattatca tttattctag
     5041 attattctat cttatttatt cggaaaaagc acaaaaaaag agcacacaat tcaaatcgct
     5101 tagggctgct ggattcctcc cctgacccgc ttcacgcaga actgttgctc cactatttat
     5161 tatatcacat tcctattcat tttaaaagca aaattatttt ttccgtctat ttctaaaaaa
     5221 atcctgcatg atagctgcgc attcatcttc caaaattcct atttcaacct ccacacgatg
     5281 attgagacgc tcatctgtca agatattgta caaactccca gcagcgccaa atttctggtt
     5341 tttagcccca tagaccacat ttggaatgcg ggcaagccca atcgctccac tacacatgac
     5401 acaaggttca atggtcacaa aaagtgtgca atccagcaag cgccagctct cctcactcaa
     5461 gttcgcatcc tctatagcca taatttccgc atgcataacc gctcgctgta attcctcacg
     5521 cgcattatgc ccacgaccaa tgatttcccc atctttgaca atcacacaac caattggaat
     5581 ttcatcgtgt tcaagagcaa tctcagcctc tctcaaagcc tccctcataa agacttcttt
     5641 ttcttcaagc gtataataca tctcttttct cttttcctac ttatcgattt tattattata
     5701 tcatgaatcc caacacaaaa aaagccaccg aatgcggtga ctctataggg agattattat
     5761 gaaaaagaaa agtttaggat atttgttaca acaagttagg aggtcttctt gtaactgtct
     5821 atagtatacc cgacctatct taaacaaatc ttaaaaatct cttaggacca aacactttct
     5881 aaaatatttg tttgttcacg accaggacct actgagaaag tagaaatacg aacgccaacc
     5941 aattcactca cacgacgaac atagttacgc gcattctcag gaagatcttc caaattgcga
     6001 actccggtaa tatcttctga ccaacctggc aactcttcat agataggctt gcaacgtttc
     6061 aattgctcaa gactagctgg atagtagtca atacgttgac cgtcaagatc ataggccaca
     6121 cagattttca cagtatccaa accgctcaaa acatcaatag agttcaaaga aaggttagta
     6181 ataccagaaa cacgacggct atgacgcatc acaactgagt caaaccaacc tacacgacgt
     6241 ggacgaccag ttgttgtacc atattcatga cccacttcac ggatacgttc tcccacttca
     6301 tcaaacaact cagttgggaa aggaccatct cctacacgac tcgtataagc tttacataca
     6361 cctacaacct tgtcaatctt gcttggaccg acaccagaac caattgtcac accaccagct
     6421 acagggtttg atgacgtaac aaatggataa gtaccttggt cgatatctag cataacacct
     6481 tgtgcacctt caaaaagcac acgtttgcca ttatcaagcg catcattcaa gataacagat
     6541 gtatctatca cgtatttctt gatttgttga ccatattcgt aatattcttc aaaaatatca
     6601 tcgaaaacaa tcgctttact gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg
     6661 ttacgttcta aacgctcacg gaaaatatct ttatctaaaa gatctgcaat acgaattcca
     6721 acacgagcag ccttgtccat ataagctgga ccaattccct taattgtcgt accaatctta
     6781 ttgtcgccct tagcttcttc ttgcaagcga tccaactcga tatgataagg caaaataaca
     6841 tgcgcacgat cagaaatacg caagttatca gttgttacac cttcctcatg aagatagctc
     6901 aactctttta caagagattt aggatttaca accataccat tcccaatgac agatattttt
     6961 tcagggaaga aaatcccaga tggaatcaag tgcaacttaa atttcttacc gtcaatcaca
     7021 atcgtgtgac cagcattatc accaccttgg taacgtgcaa tcacttctgc attcgctgaa
     7081 aggaagtctg taatcttccc tttaccttca tcaccccatt gggtacctac aacaacaact
     7141 gaagtcataa ttttgtctga gccctcaggc tcttcctttc tcacatacat ggcaggactc
     7201 tcacctgcaa
//
LOCUS       DAVMAF010000086         7149 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMAF010000086 DAVMAF010000000
VERSION     DAVMAF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.087"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            1..731
                     /locus_tag="VYO97_001830"
                     /pseudo
     CDS             1..731
                     /locus_tag="VYO97_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            792..1754
                     /locus_tag="VYO97_001831"
     CDS             792..1754
                     /locus_tag="VYO97_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1754583.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLPHQMETISKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIA
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1934..2941
                     /gene="gap"
                     /locus_tag="VYO97_001832"
     CDS             1934..2941
                     /gene="gap"
                     /locus_tag="VYO97_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1754584.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(3010..3846)
                     /locus_tag="VYO97_001833"
     CDS             complement(3010..3846)
                     /locus_tag="VYO97_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1754585.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNFPILGDPLYNSNSKTSRLMLHAFRLSFTHPLTLEKLNFTALSNTFETELKK
                     NG"
     gene            3972..6167
                     /locus_tag="VYO97_001834"
     CDS             3972..6167
                     /locus_tag="VYO97_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1754586.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6220..6372
                     /gene="rpmG"
                     /locus_tag="VYO97_001835"
     CDS             6220..6372
                     /gene="rpmG"
                     /locus_tag="VYO97_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1754587.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6382..6558
                     /gene="secE"
                     /locus_tag="VYO97_001836"
     CDS             6382..6558
                     /gene="secE"
                     /locus_tag="VYO97_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1754588.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6613..7149
                     /gene="nusG"
                     /locus_tag="VYO97_001837"
     CDS             6613..7149
                     /gene="nusG"
                     /locus_tag="VYO97_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1754589.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2256 a         1264 c         1524 g         2105 t
ORIGIN      
        1 atttatggct ggttaaagct aaaagagaaa acaggagagc taaaccacca agtaaaagga
       61 acaaaaccaa gaaaggttga tagagataga cttaaaaact atcttactga caatccagac
      121 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat ccactatgcg
      181 ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac aagagccaga
      241 aaaagtagcc ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat
      301 tgacgaaaca ggatttgata cttattttta tcgagaatat ggtcgctctt taaaaggtca
      361 gttaagaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg aatggtgagt
      421 taatcgctcc aatgacttac gaagagacga tgacgagcga cttttttgaa gcatggtttc
      481 agaagtttct cttaccaaca ttaaccacac catcggttat tattatggat aatgcaagat
      541 tccatagaat gggtaagtta gaactcttgt gtgaagagtt tgggcataaa cttttacctc
      601 ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat atcaaaaagc
      661 acctcaaaaa ggtattacca agttgcaata ccttttacga ggctttttta tcctgctctt
      721 gtttcaattg actatagttc acggatacag ttgggaaaga agctattaaa tatttggaga
      781 gtccttttgg gatgaacatg tcaaatatta ctatagatgg gcaagaaatg gatctgagtc
      841 aaacgatgcc ttcttatgct caagtagtga gtctttatga tgatacaagt atctctgtta
      901 gtaataatga gacggacaaa gtgttggctg gctccctcta tactgataca aatgaacagg
      961 gattaacgat tccaagcagt ttactaaaaa attggaatga acagacagga aaaaatttga
     1021 cagctaatga tcttattggc aaatcagtct cagccagcat tgtagaaagt gctgccgaaa
     1081 ctagtaagat tgctcaattt caaacgaaga ttgtacgtgt aatcaatgat gaagatgaca
     1141 tggaggacag caacagtttc atgctgcctc atcaaatgga aacgatttca aaagaggctg
     1201 gatttacgaa agctgtatct tattttatct tggaactcaa agatccatca cagacaaaag
     1261 tagtaacaga agaattacag aaaaataaga agtatactgt gctttctcaa cagagagttc
     1321 ttgatattgt gattaccttt attcgtgtta ttcagggatt attgattgtg ctttcatcac
     1381 aagctattgt ggtagcagcg gttatgattg gtatcattat ttacatcaat atcatgcaac
     1441 gttccaagga aataggtgtc atgaaggcag ttggctatca gaatcgtgat gtcaaaggaa
     1501 tttttattta cgaggctatc tggattgtag gaattgcctt gctactggct tttttggtag
     1561 cacaaggggt gggaagttta gcgaatgcga ttgtaagtca cttttaccca tccatcgcta
     1621 aggtctttga attaaatttg ttatctgttt taggaactct agttttcgct ctcttgcttg
     1681 gctatgtatc agcctacttc ccagcacgta aaattagtaa aatggaccct gtagaatcgc
     1741 tacgctatga atagtcaagg gtgttactag attatcgtcc ctaaaaaaga aactaacaga
     1801 gaaaaacaat gcccgtactt gcaattcaac tcaaatagtt atataatagg tttgttgaaa
     1861 ggtgatttgt agtgattcaa gttactcttt tcacaataaa aatttcatga ttttcataag
     1921 gaggaaatca ctaatggtag ttaaagttgg tattaacggt ttcggacgta tcggtcgtct
     1981 tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt acacgcatca acgaccttac
     2041 agatccagtt atgcttgcac acttgttgaa atacgacaca actcaaggtc gtttcgacgg
     2101 tactgttgaa gttaaagaag gtggattcga agttaacggt aaattcgtta aagtttctgc
     2161 tgaacgtgat ccagagcaaa tcgactgggc tactgacggt gtagaaatcg ttcttgaagc
     2221 tactggtttc tttgctaaga aagaagcagc tgaaaaacac cttaaaggtg gagctaaaaa
     2281 agttgttatc actgctcctg gtggaaacga cgttaaaaca gttgtattca acactaacca
     2341 cgacgttctt gacggtactg aaacagttat ctcaggtgct tcatgtacta caaactgctt
     2401 ggctccaatg gctaaagctc ttcaagataa ctttggtgtt gttgaaggat tgatgactac
     2461 tatccacgct tacactggtg accaaatgat ccttgacgga ccacaccgtg gtggtgacct
     2521 tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac tcaactggtg ctgctaaagc
     2581 tatcggtctt gtaatcccag aattgaacgg taaacttgac ggatctgcac aacgcgttcc
     2641 aactccaact ggatcagtta ctgaattggt agcagttctt gaaaagaacg ttactgttga
     2701 tgaagtgaac gcagctatga aagcagcttc aaacgaatca tacggttaca cagaagatcc
     2761 aatcgtatct tcagatatcg taggtatgtc ttacggttca ttgtttgacg caactcaaac
     2821 taaagttctt gacgttgacg gtaaacaatt ggttaaagtt gtatcatggt acgacaacga
     2881 aatgtcatac actgcacaac ttgttcgtac tcttgaatac ttcgcaaaaa ttgctaaata
     2941 attcttgaat cgataaaaag caaggcctct tggtcttgct tttcttatat ggaaaaatgg
     3001 atgacacaat catccattct tttttaattc tgtttcaaaa gtatttgaga gggcagtgaa
     3061 gtttaatttt tctaatgtaa gtggatgggt aaaggaaagt cgaaaggcat gaagcataag
     3121 ccgacttgtc tttgaattgc tgttatagag agggtcgccc aaaataggga agttatgatg
     3181 cgaaaggtgc acacgaatct gatgggttcg ccctgtcttt agcttgcaat gagccaagga
     3241 agtcttgttt gagaattgct ttaatctgct tacatacgtt tcagcatatt gcccattttt
     3301 tgcatcaact attctttttc tacgatcatg gcgatcacgt ccaattttgt ctctgaaaac
     3361 aagttctttt ctgttgatat ttccatcaac tagagcccaa tattctctag aaatctcttt
     3421 tttctccaat aagcgattga gaatgggcag gataaaagga tttttggcaa agagaactaa
     3481 gccactggtt tccatgtcca gacgatgaac gacatagcag gtttggccaa cataggtact
     3541 gacatggtta agaagggcaa tttcgtttgg ttgattacca tgcgttttca tcccctctgg
     3601 tttgtttaca ataatcaagt gttgatcttg ataaacttcc tgcactaagt ctgggttgcc
     3661 ccaagggatc gtcttttggg aataatcttc ctcgtcaaaa gtcaactggc aaacatctcc
     3721 aggatttacg atttcgttcc agtggacttc ttcttgattt atcaaaatat gtttcttgat
     3781 tctcaaaaaa tgacggattt ttctagggat gaggagttgt tcctcaagta attgctttac
     3841 cgtcatttga ggtagagagg cgggtaatgt aaatgtgaat tgcatacaga tattgtaaca
     3901 aaaaaagccc tatttggata ggaaatagct aaattcttgt cttcctatga tgaagatgat
     3961 aaaataaacg catgaaatta gataaattat ttgagaaatt tctttctctt tttaaaaaag
     4021 aaacaagtga actagaggac tctgattcta ctatcttacg tcgctctcgt agtgatcgaa
     4081 aaaaattagc ccaagtaggt ccgattcgaa aattctggcg tcgttatcat ctaacaaaga
     4141 ttatccttat actaggtttg agtgcaggat tgctagttgg aatctatttg tttgctgtag
     4201 ccaagtcgac caatgtcaat gatttgcaaa atgccttgaa aactcggact cttatttttg
     4261 accgtgaaga aaaagaggct ggtgccttgt ctggtcaaaa gggaacctat gttgagctga
     4321 ctgacatcag taaaaacttg cagaatgctg ttattgcgac agaagaccgt tctttctata
     4381 aaaatgacgg gattaactat ggccgtttct tcttggctat tgtcactgct ggacgttcag
     4441 gtggtggctc taccattacc caacagctgg ctaaaaacgc ctatttatcg caggatcaaa
     4501 ctgttgagag aaaagcgaaa gaatttttcc ttgccttaga attaagcaaa aaatatagta
     4561 aggagcaaat tctaaccatg taccttaaca acgcttattt tggaaatggt gtgtggggtg
     4621 tagaagatgc gagtaagaaa tactttggag tttctgcatc agaagtgagt ctggatcaag
     4681 ctgcgactct ggcagggatg ctcaaggggc cggaactgta taatcccttg aattctgtag
     4741 aagattctac taatcggcgc gatactgtct tgcagaatat ggttgcagca ggatatattg
     4801 ataaaaacca agaaaccgaa gctgctgaag ttgatatgac ttcgcaattg cacgataagt
     4861 atgaaggaaa aatctcagat taccgttacc cctcttattt tgatgcggtg gttaatgaag
     4921 ctgtttccaa gtataatcta acagaggaag agattgtcaa taatggctac cgcatttaca
     4981 cagagctgga ccaaaactac caagcaaata tgcagattgt ttatgaaaac atatcgctat
     5041 ttccgagggc agaggatgga acgtttgctc aatcaggaag tgtagctctc gaaccgaaaa
     5101 cagggggagt tcgtggagtt gtcggtcaag ttgctgacaa tgataaaact ggattccgga
     5161 atttcaacta tgcaacccaa tcaaagcgta gtcctggttc tacaattaag cctttagttg
     5221 tttatacgcc agcagttgaa gcaggctggg ctttgaataa gcagttggat aaccatacca
     5281 tgcagtatga cagctataag gttgataact atgcagggat caaaacgagt cgagaagttc
     5341 ctatgtatca agccttggca gaatcgctta atctacctgc tgttgccact gttaatgatt
     5401 tgggtgtcga caaggctttt gaggcaggcg aaaaattcgg actcaacatg gaaaaggtcg
     5461 accgtgttct tggtgtcgcc ttgggaagcg gtgttgaaac caaccctctt caaatggctc
     5521 aagcatacgc tgcctttgca aatgaaggtt taatgcctga agctcatttt attagtagaa
     5581 ttgaaaatgc tagtggacaa gttattgcga atcataaaaa ttcacaaaaa cgggtgattg
     5641 ataagtctgt agctgacaag atgaccagta tgatgttggg gactttcacc aacggtaccg
     5701 gtattagttc atcgcctgca gactatgtca tggcagggaa aactggaaca actgaagcag
     5761 ttttcaatcc ggagtacaca agtgaccagt gggtaattgg ttatactccg gatgtagtga
     5821 ttagccactg gcttggcttt ccgaccactg atgaaaatca ctatctagct ggctctactt
     5881 caaacggtgc agctcatgtc tttagaaaca ttgccaatac cattttacct tatacgccag
     5941 gaagtacctt tacggttgaa aatgcttata agcaaaatgg aattgcacca gccaatacaa
     6001 aaagacaagt acaaaccaat gataatagcc agacagatga taatttgtct gatattcgag
     6061 ggcgtgcgca aagtctagta gatgaggcta gccgggctat ctcagatgcg aagattaagg
     6121 aaaaggctca aacaatatgg gattcgatag tcaatctatt tcgctaagat gcttgtcaaa
     6181 gcctagcttt cttgttataa tggataagat ggaggcgtta tggcactaaa aaaagcaagc
     6241 ctagcttgtg cggtttgtgg ttcgagaaac tattcaatca agatcagcgg aaaccccaag
     6301 cctacacgac tagaagtaaa taaattttgt aagcattgtg gcaagtacac tacacacaga
     6361 gaaacgagat aggagagagc gatgcgtttt attggagata tttttagact tcttaaagac
     6421 acaacatggc caactcgcaa ggaaagctgg agagattttc gttctatcat ggaatacaca
     6481 gctttctttg tagtaattat ttacattttt gaccagttga ttgtttcagg tttgattcga
     6541 tttattaaca ttttttagaa gattagtgga gttaattaca ctagaaatct tctatttatg
     6601 aaaggaaata tcatggatag ttttgataaa ggatggtttg ttttacaaac ttattctggt
     6661 tatgaaaata aggtgaaaga aaatctatta caacgtgcac aaacctacaa tatgttggat
     6721 aatattctac gcgttgaaat tccaacacaa acagtgcaag ttgaaaaaaa tggaaagaga
     6781 aaagaagtag aagaaaatcg ctttccaggt tatgttcttg tagaaatggt catgacagat
     6841 gaagcttggt ttgttgttcg aaacacacca aatgttacag gatttgtcgg atcacacggg
     6901 aacagatcaa aaccaactcc attattggaa caagaaattc gtgacatttt ggtatctatg
     6961 ggacaaactg ttcaagaatt tgatttcgat gttgagattg gtcaaactgt acgtattatt
     7021 gatggtgctt ttgcagacta cactggtaag attacagaaa ttgataataa caaagtgaaa
     7081 atgattatct ctatgtttgg taatgacaca gttgcagaag taaacctaaa ccaaatcgca
     7141 gaattataa
//
LOCUS       DAVMAF010000087         6971 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMAF010000087 DAVMAF010000000
VERSION     DAVMAF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.088"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..727
                     /locus_tag="VYO97_001838"
     CDS             <1..727
                     /locus_tag="VYO97_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1754590.1"
                     /translation="YRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAVSVAITLSKKL
                     KGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFKDSVLLTPGLN
                     IVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYITIPMIKGTIK
                     TVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYARANTIGVIQIL
                     GGMLIIFCINILFRERKRLKGGK"
     gene            730..1569
                     /locus_tag="VYO97_001839"
     CDS             730..1569
                     /locus_tag="VYO97_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1754591.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            1566..2885
                     /locus_tag="VYO97_001840"
     CDS             1566..2885
                     /locus_tag="VYO97_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1754592.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRDENTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            2891..4762
                     /locus_tag="VYO97_001841"
     CDS             2891..4762
                     /locus_tag="VYO97_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV1754593.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDSQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            4784..>6971
                     /locus_tag="VYO97_001842"
     CDS             4784..>6971
                     /locus_tag="VYO97_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1754594.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVKGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLLQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLF"
BASE COUNT         2310 a          922 c         1402 g         2337 t
ORIGIN      
        1 ttataggtat gtttttaaag aatacccatt ttttacagaa gctctaatta attctgttcg
       61 ttgggctgta attggtgtta ttattcaagt tcctttagct gtatctgttg caattacatt
      121 atctaagaaa ttaaaaggct ggaagatatc taggaatctt tatattgtac caagtatcat
      181 ttctagtgct gctatgggct tgatttttct tcaaatctat aatccaaact atggtgttgt
      241 taaccaaatt attcatctat ttaatccatc gtttaaagat tcagtactgt tgactccagg
      301 attaaatata gtagctatga ctggcgctta tatcttcttt gcaggagcat caaccattat
      361 gattcttggg caaatttttg ctattccaga agaagttcaa gaagctgcta ttttagacaa
      421 tattactggt tggaggaaag agtggtatat tacgattccg atgattaagg ggacaattaa
      481 aactgtttca attatggcag caacttcagg gtttttgctc tataacgaag tattcttttt
      541 gacaaatggt gctgcaggaa caaaaagtat cagttttgtt attcgagaat tagcagtggc
      601 tagctcacga actcagtatg ctcgtgcaaa tacaattgga gttatacaaa ttttaggtgg
      661 aatgttgatt atcttttgta ttaatatttt attcagagaa agaaaaagac tgaaaggtgg
      721 gaaatgatta tgaatacaca tataaatggt attagtaaaa aaggcaaagt tcttatatat
      781 ggttatatgc tccttaccat tttaatttct attttcccta ttgcgtggat ttttttatca
      841 tcattaaaag cagatcctat gaaaaatcca ggtattagtt taccgactga ctttactctt
      901 gaaggctata taaatgtttt tacaaaactt catgttttca cttacttttg gaatagcttt
      961 aaagtagtgt ctatatcagt tattattagc attgttatga tctccatgtc ttcatatgtt
     1021 attgctagga tggaatttag agggaagaag ttggtgactt ctatgctgta ttctactcta
     1081 tttattccag caacagctat gacttttcca gtttatagat tagtaaatga gttggggatt
     1141 tataataccc cagttgctct aattctggtc tactcttgta gtggaattgc tatgagtttc
     1201 tttattataa agaactattt tgaaattatt ccaaaagaat tggaggaggc agcagagatt
     1261 gatggtgcga catatgctca aactttttgg aaagtgatgt taccaattgc tagaccagga
     1321 attttaacag cagcagtact tgcctttatt aataactgga atgaatatta ttgggcatct
     1381 atgttggtga ttgataaaaa tgaattaaca gtaccagcat tactaggtca atttactaca
     1441 agttttaata ctaattataa tggtctattt tcagctattg ttgtaatcgt attaccacca
     1501 atcattctat tcgcatttac aagtaaatac ttcattgaag ctttaggagg aggagcagta
     1561 aaaggatgaa acatacactt gaaactatca atagtagaac tcagtggttt agagaagcaa
     1621 gatttgggat gttcattcat tgggggttat attctattcc aggaaaaggg gaatggattc
     1681 gtagtcatca gaaattatct attgaagatt atgaaccata ttttcgagct tttgatccta
     1741 aggaatataa ccctagagaa tgggctaaac aagcaaaagc tgcagggatg aagtatatgg
     1801 ttttaacggc taaacatcat gatgggtttt gtttgtttga ttcaaaattt acagattata
     1861 aagcaacaaa tacacctgca ggtagagatt tagttaaaga atttgtagat gctgttcgag
     1921 ctgaaggttt aaaagtaggc ttatatttta gtttgattga ctggcaccat cctgatttcc
     1981 ctaaatatgc tgatctaaat catccaatga gaggaaatga agcctatcga gatgagaaaa
     2041 ttaactttga cagttatttg gagtatttac acaatcaagt taaagaaatt gtaactggtt
     2101 atggtcagat tgacatatta tggtttgatt attcttatga agacatggtg ggtgaaaaat
     2161 ggggagcttc aaaattaata gacatggttc gtcattatca gccaaacgtt atcgtagata
     2221 atagattaga aacatcaggt gaaggattcg gaagtatcgt tacagatgaa attacctctt
     2281 acgctggaga ttttgttagt ccagagcaaa ttgttcctca cgaaggtatt cgaaacttta
     2341 aaggtgaacc tgttccttgg gaactttgtc taactatgaa taacaattgg gcttataatc
     2401 ctacagatta tctatataag tctagtcaaa ctttgataag aaagttagtg gaatgtgtta
     2461 gtaaaaacgg aaatatgatt cttaatgtag gcccagatgc tttgggaaga attaatgatt
     2521 caagtaagaa aatattagat aattttcatc gatggatgtc tcgaaatggt gaggcaattt
     2581 atggatgtag tggagatgaa aatttaccaa aaccagattg gggatactat actcgtgatg
     2641 aaaatacggt ttatgctcat gtatttgaac aacctatagg tcctttagct cttcttggta
     2701 ttagtaaaga aaacgtaaaa agaatgagct ttcttcatga tggaagtgag gtgaaaattt
     2761 cagaaagctg gacgaccaat gcctacaaag gaatttgttt tgcacagttt ggagaagttc
     2821 cacattttac ctatccatta ccagacttga ttgatagcgt aatcaaaatt gagttgagag
     2881 agtagtttat atgaaaattg aaaataaaaa tgtaagacgt aatttctttt ggggagatgg
     2941 tcgattttat actactgata tcgtaaacaa acgggcagga gtaatgataa aaaatgtttc
     3001 gaaagaagaa tttaccatta cattggagaa tggtataaga ctttcttcaa atcatttttc
     3061 agcaatcgtt agggaagagg gagatacgcg aattcaggtt tcttttgttt gtccttccat
     3121 tcgtttacgt ttaatctttg aatcgaggga tgatgttctt tcgaagcaat tagttttaga
     3181 atcatctact gaggttatta aatctgtaga ggtagagagt tttgagtttg aaacagaaga
     3241 caatattttc tatccgaaaa gacaagattg tattaaggaa atggcgaatt tttccggtta
     3301 ttatgtagaa ttgggacaac ctgtttatgc gaattcttta ttcttaggaa tggaatttcc
     3361 aatgtctgaa aacaaggtag atggtagaca ctatgtatca agatattact tgggaactgt
     3421 tgtaaatcaa gaaaagagtt tgtggtcttg tattattggg ggagcatgtt cttataaaaa
     3481 agaagagatt caagaggcat tttttgaata tgttgaagga atagctcaac ctagttattt
     3541 ccgtaaacag tataattcct ggtatgacca tatgaccgat attacagagg aaggtatttt
     3601 aaaaagtttt tctgagattc gagatggatt tgaaaatcat ggagttcatt tagatgctta
     3661 tgttgttgat gatggttgga caaactatca atcagtttgg gaattcaatc ataaattccc
     3721 aaatggtttg agaaatatta aacatcttgt aaatggattt ggttcaagcc taggattgtg
     3781 gattggtccc cgaggtggtt ataatgggac agaaatcatt atgagtgatt ggttagaagc
     3841 acatccagag ttaaatattg gatctaaaaa tttgatttca aatgatgtaa acgtggctga
     3901 ttttaactat ctcaatcaaa tgaagaaaaa gatgttggaa tatcaaaaag aattcgatat
     3961 cagctattgg aaaattgatg gttggttact tcaacctgac aaacctgata agagtggacc
     4021 gcacggtatg tataccatga cagcggttta tgagttctta attcaactgt tgatagatct
     4081 aagaaaggag agaggaggaa aagattgttg gttaaacttg acttcttatg taaatcctag
     4141 tccatggttt ttacagtggg tcaatagttt atggattcaa atatctcaag atgtaggctt
     4201 tacagagaat gcaggtaatg atatcaatcg tatgattact taccgagata gtcagtatca
     4261 agaatttttg gaaaaacgtg agatacagtt acctatgtgg tcgctttata atcatgaacc
     4321 aatctatgct gtcagtgcaa atacctggta catggatcat caaatgtttg catcaatacc
     4381 agattttgaa gcttatctat tatttatttc aacaagaggg aatgcttttt gggagtttca
     4441 ctattctttt gatatgtttg atgaagaacg ctggaaagcc aatgctcgtg ccgtcaaatg
     4501 gatagaggag aattatcaaa cactaaaata tagtaaaaaa ataggaggaa gtcctgaaaa
     4561 atttgaaatt tatggttata agtgtcacaa tcagaagact tctactgaaa ttctctctct
     4621 gagaaatcct gctcagatta aacaaaaaat aaaaatagag aatctttcga tagagaattt
     4681 taccagagta attggagatt ttactattca agaagatgag atagaattgg caccgtactc
     4741 tatcgtgata ctgaagaagt aaagaaaaaa ttgtgaggta gagatgacga tttatattaa
     4801 taaggacgag accgtttttc atttggcaat gaaagatagt agttatattt ttagaatttt
     4861 agaaaatggg gaacttcaac atctacattt tgggaaaagg attcatgtca aggaaaatta
     4921 taaccaattg atggcctatg aaaaaagagg atttgaagta tctttttctg aagaatttga
     4981 ggatattcaa cagtctatga tacaaaatga atattcttca tatgggaaag gagattttcg
     5041 gcatccagcc tttcaagtta aaggaatgaa tggtagtagg ataacgacac taaaatatca
     5101 aggttttgaa cttgaaaaag ggaaaaatcg tcttaactct ctaccttcaa catttgatga
     5161 tattggtcag tgtgcggaaa cattaacgat tattttaaca gattccatat tagatttaac
     5221 tgttagacta aattacacaa tttttccgga atacaatgtc ttagttagaa atacggaatt
     5281 tttaaataat agcaataata agttgactct tttgaaagca atgagcttac agctagatct
     5341 acctgatagt caatatgact ttattcaatt ttctggagca tggctgaggg aacgtcagtt
     5401 atatagaact tcgcttagac caggtattca agcaatagat agcttgagat actcatcaag
     5461 tcctcagcaa aatcctttct ttatgctatc aaggagggaa actacagagc atagtggtga
     5521 ggtttatggt tttaacttta tctattctgg aaattttcaa aatatgattg aagttgacca
     5581 ttttgacacc gctagagtaa cggtaggaat aaatccagta gaatttcgtt ttttattaaa
     5641 tcctgccgaa agttttgtga caccagaagc aattgtgatc tattctgatc aagggatgaa
     5701 tcagatgagc caacaactat cagattttta tcgacatcat ttagttaatc ctaatttttc
     5761 tcaagctagt cgtcctataa tactcaatag ttgggaaaca ttttattttg acttgagtac
     5821 agaaaaaatt ttagatttag caaaggctgc taaagattta gggatagaat tatttgtact
     5881 ggatgatggt tggtttggtc ataggaaaga tgacaaaagt tctctgggtg attgggtaac
     5941 agatagaagt cgccttcctg aaggtattgg atttcttgca gatgaaattc acaaaatagg
     6001 tttacaattt ggtttgtggt ttgagcctga aatgatttct attgatagtg atttgtacaa
     6061 gaatcatgcc gattggacta tccatttgtt agacagagag aagtcagtag gaagaaatca
     6121 atatgtgttg gatttgacga gacaggaagt tgttgattat ctttttgatt ctatttctaa
     6181 aatcataatc aaaacaaatc tggattatat caaatgggat atgaatcgtc atataacaga
     6241 tatttatagt attgaacttg attctgaaca gcagatggaa tttggtcatc gatatatctt
     6301 aggtctttat cagttattag atcgtttaat aactaagttc ccttcagttc tatttgaatc
     6361 ttgttcttca ggtggtggac gttttgattt aggacttatg tattatgcac cgcaagcgtg
     6421 gacgagtgat gatacggacc cgatagaaag attgaaaatt cagcatggaa cttcttatgg
     6481 atattctcca tcaatgatga cagcccatgt ttctatttct ccaaatgaac aaagtggaag
     6541 acaaacgagt ttggacacta ggacaaatgt agcttatttt agttctttcg gttatgaatt
     6601 agatgttacg agattgtcgg tagaagaaaa agaacaagtg agagaacaaa ttcagtttta
     6661 taaaaaatat cgttcattgc ttcaatatgg ggatttctat aggataaaca gtccttttag
     6721 ttgtgattct gctagttggc aagttgtttc aaaagataaa tgccaatcga ttttattgta
     6781 tgctcaattg aatagtaagt tgaatccagg ttatacaaga gtttatttta gtggtttaga
     6841 taaagataaa tgctattccg tctctggatt tgatgagttc ttttatggag atgaattaat
     6901 gaatgctgga ataaaagtaa gtttaagtaa tttagcactt tgtgttccag aatatcttac
     6961 aaaattattt g
//
LOCUS       DAVMAF010000088         6851 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMAF010000088 DAVMAF010000000
VERSION     DAVMAF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.089"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(51..326)
                     /locus_tag="VYO97_001843"
     CDS             complement(51..326)
                     /locus_tag="VYO97_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1754595.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(433..1272)
                     /locus_tag="VYO97_001844"
     CDS             complement(433..1272)
                     /locus_tag="VYO97_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1754596.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1414..2262
                     /locus_tag="VYO97_001845"
     CDS             1414..2262
                     /locus_tag="VYO97_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1754597.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2355..3272)
                     /locus_tag="VYO97_001846"
     CDS             complement(2355..3272)
                     /locus_tag="VYO97_001846"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1754598.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3710..3915
                     /locus_tag="VYO97_001847"
                     /pseudo
     CDS             3710..3915
                     /locus_tag="VYO97_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4226..4519)
                     /gene="rpmA"
                     /locus_tag="VYO97_001848"
     CDS             complement(4226..4519)
                     /gene="rpmA"
                     /locus_tag="VYO97_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1754599.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4536..4880)
                     /locus_tag="VYO97_001849"
     CDS             complement(4536..4880)
                     /locus_tag="VYO97_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1754600.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4896..5210)
                     /gene="rplU"
                     /locus_tag="VYO97_001850"
     CDS             complement(4896..5210)
                     /gene="rplU"
                     /locus_tag="VYO97_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1754601.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5246..5333)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5428..6027)
                     /locus_tag="VYO97_001851"
     CDS             complement(5428..6027)
                     /locus_tag="VYO97_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1754602.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6494..6793
                     /locus_tag="VYO97_001852"
     CDS             6494..6793
                     /locus_tag="VYO97_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1754603.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2146 a         1387 c         1143 g         2175 t
ORIGIN      
        1 cctattgtat caagctttct agcttggtca gtaggctttt taaagactga ttatttaaca
       61 gcgtctttaa gagctttacc agctttgaat gctggtactt tagaagctgc aattgtcatt
      121 tctttaccag tttgtgggtt gcgaccttta cgttctgcgc gctcacgaac ttcaaagtta
      181 ccaaaaccga tcaattgaac tttttcacca gctgcaagat agtcagctac tgctgcaaat
      241 acagcttcaa ctgctgctgc tgagtctttc ttagtcaatt ctgtagcttc tgctacttta
      301 gcgatcaaat cttgtttgtt tgccatgtta atgattcctc caaataattt ctaattaaca
      361 aatataatca tatcctaaaa tcccttttag gtcaagttaa aaacgctatt tcttcccatt
      421 ttatttattt ttttaggagt ggtaacgtat caaaatagcc caagcgttct cacccgtgtg
      481 agtttgaata atggaacccg tttccaaaac agaaattggc ttttcaacat aagcttgtaa
      541 gctttctttc atctcttttg cccaatcatc actaccagaa tatgaaattc caatctctgc
      601 tacagcacgt tcagaaagcg atgttatcaa ctcatctaac cattttttaa atgttttagt
      661 tccacgacct ttaaccattg gctgcaattc atggtctttc atttgcatga caacacggat
      721 attgagaaga gagctcaaca agccagttac acggctaatt cgtccacctt tgacaagatt
      781 ttccaaagtt gaaacaccaa tataaagctc tgtatggttt ttaacctctt ctacatgaca
      841 taaaattgcc tccatatctt taccttcttg agctaacttc gcagcctcaa caacttggaa
      901 tttcagggct tggtcagtga aggaactatc aacaacagtc acatctgcag tagataggct
      961 agcaccttgg cgtgctgctt ctaccgtacc cgaaagagca tgggacatat gaatagcaag
     1021 aatctggcca ccatctttgc ataggtcttc aaaaatctca gcaaagacac ctacaggtgg
     1081 ctgacttgtt ttcggaagat tcttactttc ttgcatcaac tgaagaaatt taccttcttc
     1141 tttcaaatcc gcatcagaat aaacaacatt atcaatcatt acagataatg gaacaattgt
     1201 aatatctaat tgctttacta gttcaggttc aatagtaaca gatgaatcgg ttacaatctt
     1261 aatttttgtc atagtatcaa tctttctatt ttaggattca gattggtttc cttacttcta
     1321 attatatcaa aaaaaagatt aaaaatccta atggagtcaa tcaaattttc cgtaaaattt
     1381 gatataatca acttataaga aaagaggtgt cctatgatta aaaaaattta ccccattttt
     1441 accattttac taggtgctgc tatttatgct tttggactga cttattttgt agttccccat
     1501 catctctttg aaggaggggc gacaggtatt accctcatca cattttatct ttttaaaatc
     1561 cctgtttccc tcatgaacct gctgattaat attccccttt tcatcctagc ttggaagatc
     1621 tttggagcca aatccctcta ttctagttta ctaggaacct tagctttgtc cggctggtta
     1681 gctttttttg agcatattcc ccttcatatt gatcttcaag gtgatttact aatcacagcc
     1741 cttatagcgg gaatcctatt gggaattggc cttggaatta tttttaatgc tggaggtaca
     1801 actggcggaa ctgatattct agctcgtatt ctcaacaaat acactcatat atccatagga
     1861 aaactgctct ttatcttaga tttttgtatt ctcatgttga ttctcctaat cttcaaggat
     1921 ttgagattgg tttcctacac gcttttgttt gattttattg tttctcgtgt tattgatttg
     1981 attggtgaag gaggatatgc cggcaaaggc tttatgatta tcacaaaacg tcctgaccaa
     2041 cttgctaagg cgattaatga tgacctcgga agaggtgtta cttttatttc tggtcaaggc
     2101 tactatagta aagaaaattt gaaaatcatt tactgtattg tcggaagaaa tgaaattgtg
     2161 aaaacgaagg aaatgattca tcgaatcgat cctcaagcct ttataactat tacagaagcc
     2221 catgaaatcc taggagaagg cttcaccttt gaaaaagaat aaaaagaggt agtgtcgtga
     2281 cctcaaaagt tagactaaat catctatctt ttgggttaca gacaacctct tttttgtttt
     2341 atttactcaa gctcttaaga ccaattccga gttacttctt catcagcctt taactgatcc
     2401 actaattggt caactgagtc aaatttggtc atatctcgaa tgcgatcaag ccaataaacc
     2461 atgacggttt ccccataaat atcttgatta aaatcaaaaa tattgacttc aaaacgtgct
     2521 tcttctccat caaaggtcac atttttcccg acactagcca tagcacgata cttctgtctt
     2581 tgaatctcaa catcaacgac ataaacgcca tctgctggca tataagtacg gtctaaaagc
     2641 actaaattcg ctgtcggata accaattgta cgaccacgag cattaccatg aaccaccata
     2701 cctcttgatg gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt tccatctaaa
     2761 atagcttgac ggatacgagt tgaactaatc tttcctttct catcttctac aggtggaaca
     2821 atgataactt ctccatcaaa gtaatccttt aaatcttctg ctgttttttt gtcagaacca
     2881 aatgtataat caaaacctgc aacaataatt ttggcattca tagccttgat ataagttgca
     2941 aagaattctt gtgcagtgag actagcgaat tgactactaa aatcaaggag atataattct
     3001 tctacacctt cgcgctttaa ttttctttca cgttcagcag ggttcaaaat atgcaaaaac
     3061 aaatctggat gataaggctc taaagcgatc tttggagatt cattaaaggt cataacgacg
     3121 ataggcaata aatcctttct cgcagccttg ttggcaacac gaaataattc ttgatgcccc
     3181 ttatgtatgc catcaaaata gccgagaaca acgactgaat cagatggtgt gccaatatct
     3241 ttttggtttt ttataggaat agtaataatc ataaaataat tatatcatag cgatagctat
     3301 ttctggaaca gaaaatctga aatgttgttt ttttcacatg aagtgtacct gttttcaaaa
     3361 ggcactttat tctatcgttg cttaactatg aactttgcaa tattcttctc aaaaacttgt
     3421 aggacatctt caaaattttg caaggagtga ttagacttgt tcggtaacca taaagtgtca
     3481 tactatgctt atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat
     3541 tggtgccgaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa
     3601 tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc
     3661 actttccctg atgacaaaga aaaaagccaa agcaataaag aaaaactaga tgactgcttt
     3721 ccaacaactc ccatctagtg tgcttcagac tgggctattt ttctttccat ctgttagctt
     3781 ggattctcag accgtttcag ctaaagaata tcttttcctt tatcagaagg aacggctcaa
     3841 gccattcaga caagtgaagg gacgacaagc caatatttga aaccaggtag cagttcttat
     3901 agtcaattga aataaaatct gaagaaatcg agtaggaaac ttaaatcaat ttttagcagt
     3961 gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag
     4021 gctgtacttg agtacggcaa ggtgaaactg acgtggtttg aatttgattt tcgaagagta
     4081 tcaatccact atacatatta aaaagtatgg gtaccaaaac aaaaaaacag gctctccgaa
     4141 aactcggaaa gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc
     4201 gggataaaag gttcaatgga cctttttatt tagcgattgg gtaaacagaa acttgttttt
     4261 tatcgcgtcc tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt
     4321 ctccaccacg accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga
     4381 ttgatccacc tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg
     4441 aatcacgtcc gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt
     4501 taagagtcat ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt
     4561 tggacgaact cagaatagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag
     4621 aataactggg tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga
     4681 tagccacctt catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata
     4741 gaattgataa agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat
     4801 tcgccactct cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc
     4861 tcaaagactg cttgtatcat gtgttctcct taaaattaag cgttaattgc gttgatgaca
     4921 acttttgtat atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac
     4981 ttgtaagtaa caactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct
     5041 ccagcaacaa gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta
     5101 aaagtaactt cttgaccagc ttcaacgttc aatttttcaa tgtaaactgc ttgaccaact
     5161 tcaactttaa cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc
     5221 ctatgatttt tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacaa
     5281 accacttaaa cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata
     5341 gtcaactctt caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatca
     5401 agctttttct tttacttttt tctgccacta ggcaaaaagc atactgagat aaaaaatgct
     5461 catcataata ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga
     5521 aaagacaacg ccaaggacaa aactactaag caggctaaca aatatgaacc cttcacgcaa
     5581 aaaaggagtg tgcttagttc ggaaataatc tccaaaagct agcattgttc gtttgatatt
     5641 ccctgtcata aaggcattat tgtaggcaat acccgaaacc tcaccaaaag cagttgtcac
     5701 cagtcccata cagaaggcca agggctgcac tagatagata ttatctacag tttgtggcac
     5761 aaaaccaata attattgata ggattgctaa aggaatcaag gacagaatag gttttttcac
     5821 aattctcaat ttttccttat aaatcgttaa taaaaagact cccattataa acgttattaa
     5881 ggttaaaagc ttatccctaa catccgaaac attattttgt attagctcta ccgaaagaaa
     5941 gacaacattt ccagcttgtc cagctacaag ggtattccct cgaacaataa aagtgtaagc
     6001 atccacatat ccagcacaaa atgtcaaaaa agtgctaacc ttttagactg acgtgatatt
     6061 tttcttatag gtaataacct cattttccct cccattgtat tttctcttag aaatattgta
     6121 ccatttcttt ctaaaaaatc gtagactacc atttagattt tactattagc ataaaaataa
     6181 tagacaacta tttatccaaa aatagataga ggtaacatgt ttgcaaacaa agcatacgaa
     6241 cctttagtaa aatcatttcc atgaaactag aatagagccc ctcttagcaa aaatcattat
     6301 tttaatttat ttctaatcac tccttgacat aaataactct caccaataaa agactatgtc
     6361 ttaaaaaaat ggtataataa aatcaatact tgggcttgat ggctatgcta ctaataacaa
     6421 ttaggagaga aaatcaggca cttgttaaca acaaggatta tccccttgag atgaaaggaa
     6481 ctttagaaat cttatgatga acatgcaaaa catgatgcgt caagcacaaa aacttcaaaa
     6541 acaaatggaa caaagccaag ctgaacttgc tgctatgcaa tttgttggca aatctgctca
     6601 agatcttgtc caagcgacct taactggcga taagaaagtt gtcagcattg atttcaatcc
     6661 agctgtcgtt gacccagagg accttgagac tctttctgat atgaccgttc aagccatcaa
     6721 ctctgctctt gaacaaatcg atgaaactac caagaaaaaa ctgggtgctt tcgctgggaa
     6781 attacctttc taaaaacaag gagctagaac aatgcttgtc gataacaaag gctaagaaag
     6841 gtgcaaaaat g
//
LOCUS       DAVMAF010000089         6713 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMAF010000089 DAVMAF010000000
VERSION     DAVMAF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.090"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(117..1985)
                     /gene="pspA"
                     /locus_tag="VYO97_001853"
     CDS             complement(117..1985)
                     /gene="pspA"
                     /locus_tag="VYO97_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV1754604.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEESPVASQSKAEK
                     DYDAAKKDAKNAKKAVEDAQKALDDAKAAQKKYDEDQKKTEEKAAREEETAKKVNDAA
                     LAQQKAYAEYIKANQEQGRVRKEKLAEAQRKIDEATKKLEEAKKEFQTVRQTIVIPER
                     EKLAETKKKAEEAEQKEPELTKKLEEAKVKLEEAEKKATEAKQKVDAEKYALETKIAE
                     LEYEVQRLEKEIKEIDESDSEDYLKEGLRAPLQSELDTKKAKLSKLEELSDKIDELDA
                     EIAKLEKDVEDFKNSDGEQAEQYLVAAEKDLDAKKTELEKTEADLKKVANEPETPAPA
                     PKPETPAPAPEAPAPAPAPKPEQPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEE
                     YNRLTQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNN
                     GSWYYLNSNGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEVSGAMKASQWF
                     KVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATG
                     WVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYEVNANGEWV"
     gene            complement(2271..2774)
                     /locus_tag="VYO97_001854"
     CDS             complement(2271..2774)
                     /locus_tag="VYO97_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754605.1"
                     /translation="MKLLDCILDYQERFNRKACQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFIGDIFYFKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            complement(3908..4636)
                     /locus_tag="VYO97_001855"
     CDS             complement(3908..4636)
                     /locus_tag="VYO97_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754606.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            complement(4633..4986)
                     /locus_tag="VYO97_001856"
     CDS             complement(4633..4986)
                     /locus_tag="VYO97_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1754607.1"
                     /translation="MELVLPNNYVVIDEEEMMYFDGGAYLSKRACQGICVALAMSPGT
                     FIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(5042..5944)
                     /locus_tag="VYO97_001857"
     CDS             complement(5042..5944)
                     /locus_tag="VYO97_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754608.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLEFPLDFVISMSL
                     AFCSLILSKIALDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSV
                     DDLKSRMIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(6152..6526)
                     /locus_tag="VYO97_001858"
     CDS             complement(6152..6526)
                     /locus_tag="VYO97_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1754609.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT         2093 a         1324 c          976 g         2320 t
ORIGIN      
        1 ctgaaatcat aagacaggta cctaaagaat acgaacataa atctattcaa tcgaacctta
       61 tctttgtttc aaaattaaaa tgtcaaatgt tcttaacatg atttaattta atcggcttaa
      121 acccattcac cattggcatt gacttcatag ccatctacag ttgtgttgac tgcaagggca
      181 cctaagccat tgacatagta ccatttatct gatactttga accattggct tgctttcata
      241 gcacctgatg cttcaagata gtaccaggta tctccatctt tcacccaacc tgttgccatt
      301 gaaccgttag cgttgaggta gtaccatgaa ccgttgactt tagcccaacc tgttgccata
      361 gcaccgttag cgttgaggta gtaccatgaa ccattgtatt ggagccatcc tgtcgccata
      421 gcgccattgc tgttgacata gtaccattta tctgatactt tgaaccattg gcttgctttc
      481 atagcacctg atacttcaag atagtaccag gtatctccat ctttcaccca acctgttgcc
      541 attgaaccgt tagcgttgag gtagtaccat gaaccgttga ctttagccca acctgtcgcc
      601 atagcgccat tgctgttgag gtagtaccat gaaccgttgt tttgtagcca acctgtagcc
      661 attgaaccat cagtattgta gaagtaccac ataccgtttt cttgtttcca gcccgttttt
      721 ggtgcgggag ctggttgctc tggttttggt gcaggagctg gagctggttt ttctgctttt
      781 ggcggttgct gttgagtcaa gcggttatat tcttcttctg atctacgagc atagtcttct
      841 tcagcttgtt gatctgctga cttctctggt tttggtgctg gagctggttg ctctggtttt
      901 ggtgcaggag ctggttgctc tggttttggt gcaggagctg gagccggagc ttctggagct
      961 ggggctggag tttctggttt aggagctggg gctggagttt ctggctcatt agctactttc
     1021 ttaaggtccg cttcagtttt ttctaattca gtttttttag catctaagtc tttttcagct
     1081 gcaacaagat attgttcagc ttgctcaccg tctgagtttt tgaaatcttc tacatctttt
     1141 tcaagttttg caatttcagc gtctaactca tcaatcttat cactcaactc ttcaagtttt
     1201 gatagtttag cttttttggt atccaattca gattgaagag gagcacggag gccttcttta
     1261 agataatctt ctgagtcaga ctcatcaatc tctttgatct ctttttctag tctctgaact
     1321 tcatattcca actcagcgat tttagtttca agagcatatt tttctgcatc cactttttgt
     1381 ttggcttcag tagctttttt ctcagcttct tctaatttta ctttagcttc ttctagtttt
     1441 ttagtaagtt ctggttcttt ttgttcagcc tcttctgctt ttttcttagt ctcagccaac
     1501 ttctctcgct caggaattac tattgtttgt cttacagttt gaaactcctt cttagcttct
     1561 tctagttttt tagtcgcttc atctattttt ctttgcgcct ctgctaattt ttccttacgg
     1621 accctacctt gttcctgatt agcttttata tattctgcat atgctttttg ttgcgctaaa
     1681 gcagcatcgt ttaccttctt agcagtttct tcttctcgcg cggctttctc ctcagttttc
     1741 ttctgatcct cgtcatattt tttctgagca gcttttgcat catctaaagc cttttgagca
     1801 tcttctactg cttttttcgc attcttagca tctttcttcg ctgcatcata gtctttctca
     1861 gctttagact gactagctac gggagattct tctgctctta caaaagtagg ctgagacgca
     1921 acaaaaccag cccctaagat agcgacgctg gctagacttg ttaaaatcat ttttttctta
     1981 ttcatctaaa tttacctctt ttctgatagt catttttaga ctatacttat attaagtata
     2041 ctccccccgt aaatatttgt caaatgtttt atattacatt tttattacaa atttctatat
     2101 cataagcttc tactccaaaa agataaagac actacttttg cccttttcga ttctcttttt
     2161 aaacatagca tcatcttcct tcattcctat aaaaacactt ccgatgagag agcttgtttt
     2221 ttaatttcgt taaacaacaa aaaacgcaag cttaagcctg cggacaatct ttatagccat
     2281 ttcatttctt tcacatctgt tttacgaact ttagcatatt tcttagcgct tgtaaaatgt
     2341 agagtaactg gtttaaatac tccgcttttg tctcttcgta aacctaaaac tgcggatctt
     2401 ttatttttat cctcaaaaaa tatgacgtcc aatttcatag tatttttaga taaatcctta
     2461 gctacgatgc aacaacttgt tattttaaaa tagaaaatat cacctataaa ctcatataaa
     2521 gatatccttt cgattacatc attgtacaat aggatatttc taaaatcttc taaaataaaa
     2581 acaccagctt gaatagcagg ttttgtttga ctagcataat ctcgtgtgat tttgtgtaag
     2641 ccaaataaat gatgtaaatc agtcaggcaa aaattcactt tgaaagtctc taaatactta
     2701 taattcgttg atacttgaca tgcttttcta ttgaatcttt cttgataatc caaaatacaa
     2761 tcaagtaatt tcaacgacaa actccatatt ttctcaaaat aaaaaagcac ggggcagttc
     2821 tcactactgc ccaaagtggt tgattagagt cggcttctct aactccctat gttgtgctta
     2881 ttaggcttat cacaagggaa cagtttttag ctctgccaag ccacatagtg tgcttactac
     2941 tatgtgtcca tgtcaccatg aattgccgaa cggtttaacg agaaagtata gtagattgaa
     3001 acaagatgtg aacaaatcga ttaggaaagt caaattaatt tctagaaata ttttaacagt
     3061 cataacggac tattccagat tcaatctact atattacttt taggtttatc ccaactcaat
     3121 tatgacatct ttttttcaaa agtcaatata tctcactctt ttcaacgaca agaaagaggc
     3181 tgataatcga ccaacctctg tttttagata cagttcaagt ccaaactatt ctttatagaa
     3241 aagtccgata aattcgagca atagttaaca tacttacaaa tataactcct aaaatagata
     3301 acactatttt cacaaaactc agttccgaac ctatgacata tatatgaaaa agaggggcta
     3361 gtcctacaca tatatacaat aagtagccaa caagttttat ctcaccaatt cctgcaaaaa
     3421 aatgtttcat gtaacgattc tccatttact ccataaatca gttttatttt agactatcat
     3481 aaaacaatca cctcttcaat aaaaaccatt ggcgaaatac tattctgaag cattgatagc
     3541 tcagatttct cagaataaag ctgtcctaac tatcgagcag ctcaaataca tatatatgag
     3601 caattctaag cctagaattg ctataaaata aatgttttcg ggctattcta agtctttgac
     3661 tgcttaaaat tttacattct gaaggattct aaacctagaa tagctcaaaa tcacgagaac
     3721 taaggtattc gaaacttcag agtaatacaa tccccatagg aattctccat tcttattaag
     3781 catgattaaa atgtaatcct aacaagtgat cctcgactgt gtttgtagca cttagttacc
     3841 tagataagac tatttccctt tatcatatct gattttaagc cgttctaaga tttaacctat
     3901 gtttctatta cttgctattt ttaaataatt cgaattcctt ttctttgata aatggaactg
     3961 aaaaattaga aaaaagaagt aaaaccgaaa acaaatagcc tgctttttga atcactaaaa
     4021 gatatcctaa aaataagtaa gctataacat ccaatgcgaa aagaaaaaat attctcctct
     4081 tcattaaagt acttgtgtca aataatctga tgaataaaaa ttttatcgta gtatctacac
     4141 aaatttttat caaaaagata agagccaata cacgattatc aactgtaccg taaagaatag
     4201 atggaaatat agttaatata aaactagaaa tctctaaata ttttatccaa ggagattgag
     4261 acaccttgct tctaaactga ctaattttct tatattcgtt agtcaattta gtagcaaaat
     4321 cttccccaat taaatgaaag attgctaact ctaggataaa aacacttatt aatacggtat
     4381 aagtcaaaaa actttctggg aaaaacttat tgattaaaat ggggaaactc attaacagaa
     4441 tcgaatttat ggctagacag atagatacta ttttattttt cacagaatat agaggtagtg
     4501 atactattga gtagataaaa gctaaaaaaa taagcaaaaa atactccgtg tcatttataa
     4561 tcgccctgaa tgataaaaat atatacatcc aacatacgat aatatgtatt tttcgaatct
     4621 tatctacatt attcatctta ctgtcgcaga tataaatcca tcccaaggat aagggttccc
     4681 gctaatatca caacctctat taagagcacc atatccaata cagtaagcta tttttcctgc
     4741 tgctgtagcc aatacacctg ctgctgcacc aataagccag cctccaagac ctccaacctt
     4801 aatatagttt attagttttt tggttaaaac tgcagctcca gtcaatgcta taaaagttcc
     4861 tggactcata gctaaagcta cgcaaattcc ttgacaagca cgcttgctta aataagctcc
     4921 cccatcaaaa tacatcattt cttcttcatc aatcacaaca taattatttg gtaatacaag
     4981 ttccatttgt tttctcctcc ttagttgaaa ttgtttcatt tgttttacaa ataggacgaa
     5041 cttaaactat ctttttatta ctcagtaata atttccgaga tttcatttta tgatactctc
     5101 tattcataca gaaatcaaaa ttttgcctta accaacttgt ataatagaaa aaatctaatt
     5161 gattgattct gatgaggtgt atcctaattt tatcaacctt ttcttgccag ttgttattgt
     5221 tactaataga aatctcatac agcagtaact cgacaagacc tgtacacaga agggtgttat
     5281 gagaatcttc aattttactc agaatatcct caaaaagttc actatccgtt aaaaaggtgg
     5341 attggttcat cgcatcatac agctgaagag acattaagtc agaataactg agatctgatt
     5401 tttgtatctt atcctttaac gttttcgtca aatcgctttc aaacgaatct tctttttccc
     5461 atttattttg attaaaaaga aattccgtct tattaaaaac gagatgattg atatccatta
     5521 tagtccagaa aaatattgta gtaagcatga tgattgcaaa aggaattttt aaaataaata
     5581 ctaggataat agctatttca agaataaaac tccatatcat tctagatttt aaatcatcaa
     5641 ccgacaaact ttttcgatgg aatgaaaaat agggtgagaa cctcacattt actttcttcc
     5701 tgcctataaa aataaaagga aaaatcgtaa taagagcttc tttcttgtac aaatctttca
     5761 atgcgtatag tatgtctaaa gctatttttg ataaaatcaa actacagaaa gcaagactca
     5821 tactaatcac aaaatcaagg ggaaattcta gattcaatag taagttattc actaggacat
     5881 gcaactctag ccacaagaca aaaagaggga gcattagtaa gaaaacaatt agtttacgca
     5941 tcataatttt acagtgtcct tatcattttc ttaattttgg ataaattatc attgctatga
     6001 ttaaaatcaa aaaaacgaca ttaatccaaa taatcaactt ttttatttct tcgctaacaa
     6061 aatcataggc taaaaaagtt atggatgata taaatagaca tacaaaaaaa ataataacaa
     6121 aaattttttc cattttaata cccatatcta gttaccacaa ggcgtttaaa tttgggtctc
     6181 tcctatttac atcattgttg gcccagattg ttgtaatagc atcgccaatt gacatccaag
     6241 ctgcaaatcc attaatagca gacagtgcaa atcctgccac tctatttgca actcgtaccc
     6301 caacatatct tgcagctgct ccagcaattg cgcttgtaac tcgccgcaaa ccataattac
     6361 gaatataatt tctaactaga ctgattgctc ctccacctaa aacaccatta aaagctatat
     6421 taatggctgt tgcaacaggc catctcagaa tagaaaatcc cccatcaaga tacatcatct
     6481 cttcttgctc aagagcaaca taattatttg gtaatacgag ttccatttat tttctccttt
     6541 tttattaagc atagttagga tagtttccta acaagtgata gacgactgtg tttttatcac
     6601 ttagctacct atataagata tcctttctct accttatttg tttttatagg ttacctaagg
     6661 tttaaaatca attattgttt gaaacttaac agttctaaac cactagcaat aaa
//
LOCUS       DAVMAF010000090         6693 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMAF010000090 DAVMAF010000000
VERSION     DAVMAF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.091"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            402..1262
                     /gene="speE"
                     /locus_tag="VYO97_001859"
     CDS             402..1262
                     /gene="speE"
                     /locus_tag="VYO97_001859"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1754610.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            1259..2518
                     /locus_tag="VYO97_001860"
     CDS             1259..2518
                     /locus_tag="VYO97_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1754611.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            2518..3645
                     /gene="nspC"
                     /locus_tag="VYO97_001861"
     CDS             2518..3645
                     /gene="nspC"
                     /locus_tag="VYO97_001861"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1754612.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            3642..4727
                     /gene="aguA"
                     /locus_tag="VYO97_001862"
     CDS             3642..4727
                     /gene="aguA"
                     /locus_tag="VYO97_001862"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1754613.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            4737..5612
                     /gene="aguB"
                     /locus_tag="VYO97_001863"
     CDS             4737..5612
                     /gene="aguB"
                     /locus_tag="VYO97_001863"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1754614.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(5793..6602)
                     /locus_tag="VYO97_001864"
     CDS             complement(5793..6602)
                     /locus_tag="VYO97_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1754615.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         1956 a         1333 c         1498 g         1906 t
ORIGIN      
        1 gttgagctgt cgaaaagaca gatctaagag ttcaaaacca gctttattat ttgaaaaagt
       61 gagtggttta agaacagttt tcctcgaaca ttcaaggctg taacatcgtg tttattttta
      121 gcgacatcaa tacctacata aagcatggga gtacctccag atatagtatt tcaagtctac
      181 ttggttatcc acgaactttt tgccttgtta ccttagacga gataaaacgt ctatgcgtta
      241 tcaaactcat taccaattga aacaaaaagc tgtggttaga gcctttcgga aatcgttaag
      301 cgattggagg aaatgaacta atccacagtg acttattcca agtataccac ttgggctttg
      361 gcagtagcta actgcgctaa atataatata aggagaaata gatggattta tggttttctg
      421 aagttcatac tccagatgtc aaattgtctc tgagaacagc caagcaactt tacgctggaa
      481 aaagtgaatg gcaggatatc gaagtcttgg atacgccagc ttttgggaaa atactgattt
      541 taaatggcca tgtcttgttc tcagatgcgg atgatttcgt ctacaatgaa atgaccgtcc
      601 acgttcccat ggctgtccac ccaaatccaa agaaagtatt ggttattggg ggtggtgacg
      661 gcggtgttgc ccaagtatta acactctatc ctgaactgga acaaatcgat atcgtggaac
      721 cggatgagat gttggtcgag gtctgtcgtg agtatttccc agactttgct gcagggctag
      781 atgatcctcg tgttaccatt tactaccaaa atgggctacg ctttttgcga aactgcgaag
      841 atgattacga tattatcatc aacgatgcga cagatccatt tggccatacg gaaggactct
      901 ttaccaagga attctacggc aatagttatc gagctctgaa ggaagacggc atcatgattt
      961 accagcatgg gagtcccttc tttgacgagg atgagtcggc ctgccgaagc atgcaccgca
     1021 aggtcaatca agcctttcca atcagtcggg tttatcaggc ccatattcca actagcccag
     1081 ctggctattg gttgtttgga tttgcatcga aaaaatacca ccctgtcaaa gattttgaca
     1141 aggaaggctg gaaaaaacgc cagcttttca cagaatacta cactgcaaac ttacacgtgg
     1201 gagcctttat gttgcccaag tatgttgagg acattttaga agaagaggaa ggaaaaaaat
     1261 gagtcgttta ctagttattg gttgtggggg cgttgcccaa gttgctattt caaagatttg
     1321 tcaagatagc gaaacattta cagagattat gattgctagc cgtaccaagt caaaatgcga
     1381 tgacttgaaa gcgaagctag aaggcaaaac aagtactaaa attgaaactg cagcacttga
     1441 tgctgacaag gtagaagagg tcattacctt gattgaaagc tacaaaccag aagctgtttt
     1501 gaatgtagct ctgccttatc aagatttaac cattatggat gcttgtttgg caacaggtgt
     1561 tcactatatc gatacagcca actacgaagc agaagacaca gaagaccctg agtggcgtgc
     1621 tatctacgaa aaacgttgta aggaacttgg ttttacagcc tactttgact actcatggca
     1681 gtgggcttat caagagaaat tcaaagaagc aggcttgact gctcttcttg gttctggttt
     1741 tgacccaggt gtaaccagtg tcttttcagc ttatgccctc aaacactatt ttgatgaaat
     1801 ccattatatc gacattttag actgtaatgg cggtgaccac ggttatccat ttgcaaccaa
     1861 cttcaaccca gaaattaatc tccgtgaggt ttctgcgcca ggttcttact gggaagatgg
     1921 gaaatgggtc gaagtcgaag ctatgtctat caagcgtgag tatgatttcc ctcaagttgg
     1981 acaaaaagat atgtatctcc ttcaccatga agaaatcgaa tcattggcca agaacattcc
     2041 aggtgtcaaa cgcattcgtt tctttatgac ttttggtcaa tcttacttga cgcacatgaa
     2101 atgtcttgaa aatgttggac tccttcgtac ggataccatt aactttaacg gccaagaaat
     2161 tgttccaatt caatttttga aagccttgct tccagatcct gctagccttg gcccacgtac
     2221 tgtaggtaaa accaatattg gatgtatctt tacaggtgtc aaagatggcg ttgaaaagac
     2281 tatctacatc tacaatgttt gcgaccatca agagtgctat gcagaagtcg ggtcacaagc
     2341 aatttcatac acgacaggcg ttccagccat gattgggaca aaattagtca tgaacggaac
     2401 ttggaaacaa gctggagtgt ataaccttga ggagttagat ccagatccat tcatggaagc
     2461 tttgaatgag tatggtttgc catgggttgt ggttgaaaat ccacaaatgg tggactaatg
     2521 aagttagaac aagtaccaac accagcctat gttattgact tggccaagtt agaagctaat
     2581 tgccgcattc tacaatatgt acaagaagag gccggttgca aggtcttgct tgcccagaag
     2641 gcatattccc tctacaaaac ttatcccttg attagccagt atctatcagg tacgacagct
     2701 agtggactct atgaggccaa attggcaagg gaagaatttc ctggtgaagt ccatgttttt
     2761 gcaccagctt tcaaggatga ggacatggag gaattgcttg ggataacaga ccatatcgtc
     2821 tttaactcag agagacagtt gcgtaagcat ggggctcgtt gtcgagaggc tggtgtcagt
     2881 gttggtttgc gcctcaaccc tcagtgttca actcaaggag atcacgcgct ctatgaccct
     2941 tgtgcacctg gttctcgctt tggagttact atagacaaga ttccgagtga tttgctagat
     3001 ttggttgacg gacttcattt tcataccctt tgcgagcagg gagcagatga tttacaaaca
     3061 actttgaaag cagtagaaga acagtttggt ccctacttac atgaggtaaa atggctcaat
     3121 atgggtggtg gtcatcatat tacaagagaa ggttacgatg tggatttgct gatttcagaa
     3181 atcaagcgta tccgaaaaac ttacaatctt gaaatctata tcgagcctgg tgaagccatt
     3241 gcgcttaatg cgggttattt agcaactgag gtattagata ttgtagaaaa cggtatggaa
     3301 atcttggttt tagacgcctc tgcgacctgc catatgcctg atgtacttga gatgccctat
     3361 cgtccacctt tgagaaatgg ctttgagtca caggaaaaag cccataccta cagactttct
     3421 tctaatacct gtctgactgg cgatgtgatt ggtgattata gttttgaaaa tccagtccaa
     3481 atcggagaca gactttattt tcaagacatg gccatttatt cttttgtcaa aaataatacc
     3541 tttaatggta ttggattgcc aagtctctat ctcatggacg aacagggaga ctgtagctta
     3601 ctcaaagctt ttggctatca agactttaaa gggagattat catgatggac agtccaaaaa
     3661 aattaggcta tcacatgcca gcagagtacg aaccccatca tggtaccctc atgatatggc
     3721 cgactcgacc aggatcatgg ccttttcaag gaaaggctgc taaaagagca tttactcaga
     3781 tcatcgagac catagcagaa ggggaaagag tctatctttt ggtggagcag gcctatctat
     3841 ctgaagccca atcctatctt ggagacaagg ttgtttattt agacattccc accaatgatg
     3901 cctgggcgcg tgatactggc ccaaccattc tcgtcaatga taaaggtaag aaattagccg
     3961 tggattgggc ctttaatgct tggggaggca cctatgatgg tctttatcaa gattatgaag
     4021 aggatgacca agtagccagt cgttttgctg aggccttgga aaggcctgtc tatgatgcta
     4081 aaccttttgt actggaagga ggcgcaatcc atagcgatgg tcaaggaact attctcgtaa
     4141 ctgaaagttg cttgcttagt cctggtcgca atcctaactt gactaaagag gagattgaaa
     4201 acacattatt agaaagtctt ggtgctgaaa aagttatttg gcttccttat ggtatttatc
     4261 aggatgaaac caatgaacac gtcgataatg ttgttgcctt tgttggtcct gctgaggttg
     4321 ttttggcttg gacagatgac gaaaatgatc cccagtatgc catgtcaaaa gcagatctcg
     4381 aactcttaga acaggaaaca gatgcaaaag gttgtcactt caccattcat aaattgccta
     4441 tccctgcagt tcgacaagtt gtgacagaag aagatttgcc aggctacatc tatgaagaag
     4501 gagaagaaga gcgatacgca ggtgaacgac tagcagcttc ctacgtaaac ttttatatcg
     4561 ccaacaaggc tgtcttggtt ccacagtttg aggatgtaaa cgaccaagtg gccttagata
     4621 tcctcagcaa gtgtttccca gaccgtaaag ttgtcggaat accagccaga gatattctct
     4681 taggtggtgg caatatccac tgtatcaccc aacaaattcc agaataggag aaaaagatga
     4741 gaaatgtaag agttgcaacc attcagatgc aatgcgctaa ggatgtggca acaaatatcc
     4801 aaaccgcaga gcgtttagta cgtcaggctg ctgagcaagg agcccaaatt attctcttgc
     4861 ccgagttgtt tgaacatccc tatttctgtc aggaacgtca gtatgactac taccagtatg
     4921 cccaatctgt agcggaaaat actgccattc agcattttaa ggtgattgct aaggaactac
     4981 aagttgtttt accaatcagt ttctatgaaa aagatggtaa tgtcttgtat aactctattg
     5041 ccgtcattga tgcagatggg gaagtgctgg gcgtttatcg aaagacccat ataccagatg
     5101 accattatta tcaagaaaaa ttctatttca cgcctggtaa cactggtttc aaggtctgga
     5161 atactcgcta tgctaagatt ggtatcggta tctgttggga tcaatggttc cctgaaacag
     5221 cgcgctgtct tgcattgaat ggtgctgaat tgctctttta tcctacagct atcggttcag
     5281 agccaatttt ggatacagat agttgtggtc actggcaacg tactatgcaa gggcacgcag
     5341 cagcgaatat tgttccagtc atcgcagcca atcgttatgg tttagaggag gttactccta
     5401 gtgaggaaaa tggcggacag agttccagtc ttgacttcta cggttcctcc tttatgacgg
     5461 atgaaacagg agctattcta gaacgagctg aaagacaaga agaagctgtt ctgttagcta
     5521 cttataacct agacaaggga gcaagtgaac gcctaaactg gggcttgttt cgagatagaa
     5581 gaccagaaat gtatagacaa attacagatt agtgtgggag aaatgagaga ttcattctgc
     5641 tagactaact tcttattagc aactataaga tactatggca tctagtaaat cgatttttat
     5701 gattcgctat tcttctctat tgattagtcc gtattttaaa atattagcaa aaaagcaaat
     5761 agcagtaact tctgtctatt tgcttttctt ttttatagaa tatatttctc aatagcacgc
     5821 gcaacgccgt cttcttcgtt gcttgaggta acggcatccg caagagattt gacataatcg
     5881 ctggcatttc ccattgcaat cccaagccct gcaaactgga gcatttcgat atcgttatta
     5941 gcatcgccca tggccataat ctctgaggaa tcaatcttca aaatctcagc tagtcgtgaa
     6001 agagcagtag cctttgtcgt tccaagtggc attgcttcat aaatgacagg ctgcgaacga
     6061 actccactga atcgttggca aagctcttca gcaaaacgct gctcaaaatc gtctgtttgt
     6121 tcttttgttc ctaaaaacat accttggaac atccggaact ttccactagt cgcttcctca
     6181 agagaaattt cagtcaggtc tgaaaatact agtttagcat cattttcaat aacttgattg
     6241 ggctttccac cgagaacaaa ataatgtgac tcgtcaaaaa gtgtcaactg aacatcactc
     6301 ttttcagcaa ggtcatagag gtattcgatg tcagctggac taagttcttg ccagtcaacc
     6361 aagccccagt cgctagtctg gtgagttgaa caaccgttgt taacaatcac atactcattc
     6421 tggaggtcga gtcccagttt tttgtagtag gggaggacac cgaaaagggg gcgacccgta
     6481 cagagaacca gtttgacacc tttttcaatg gctttgtgaa tagcagtaat gtgagcttgt
     6541 gggatttcct tggcttcatt gaggagggtg ccgtccatat ccaaggctag tagtttaatc
     6601 ataggtcttc cttctttatc ttttgctatt attatagcat attttggaga agaaattgat
     6661 agaaagcttg agactaattg attttatagt tta
//
LOCUS       DAVMAF010000091         6507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMAF010000091 DAVMAF010000000
VERSION     DAVMAF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.092"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(43..486)
                     /locus_tag="VYO97_001865"
     CDS             complement(43..486)
                     /locus_tag="VYO97_001865"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1754616.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(516..2738)
                     /locus_tag="VYO97_001866"
     CDS             complement(516..2738)
                     /locus_tag="VYO97_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1754617.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            3697..4326
                     /locus_tag="VYO97_001867"
     CDS             3697..4326
                     /locus_tag="VYO97_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1754618.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(4449..6341)
                     /gene="pepO"
                     /locus_tag="VYO97_001868"
     CDS             complement(4449..6341)
                     /gene="pepO"
                     /locus_tag="VYO97_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1754619.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1772 a         1534 c         1269 g         1932 t
ORIGIN      
        1 atgatagatg agaaagacca agccgactgg gcttggtctt tcttatctct ttttagtatc
       61 taggataatg gtaacaggtc cattattaac cagctcaacc tgcatatctg ctccaaagat
      121 gcctgtctga acgggcactt cttgcgctaa tttttgattg aaagcatcat agaagtctga
      181 tgccatatca ggtttagctg cccctgtaaa ggctggacga ttgcctctct tagtatccgc
      241 aaagagggta aactgagaaa tagagaggat ttctccttca atatctttga cagacaggtt
      301 catcttgcct tctgcgtctg aaaaaatccg catattgacc agttttctca cagcatagtc
      361 caaatcttcc tcttggtcct ctggtccaac accaaccagc aataaaagtc cctgattgat
      421 ttttccctga atctggcctt ctatactcac ttgggctttt ttaacccgtt ggataatgat
      481 tttcataata gcctttctag taagagctag gacaactagc cgttggtccg tttgacagag
      541 taaacttctg gcacactctt aattttatcg acaaccgtgg tcagtgtaga gaggttggca
      601 ataccgaagg acacatggat attagcaaac ttcatatcct tggttggttg ggcattgacc
      661 gttgaaatat tcttggttgt atttgaaaga acttgcagta catcgttcaa cagtcctgta
      721 cggttgagac cgtagatatc gatatgggcc atatactcct tatttgagct agggtactgg
      781 tcttcccatt ccacatcaag gagacgttgc tcgtagtttt cttgggcacg caggttcata
      841 cagtccacac ggtgaatagc cacaccacga cccttggtaa tgtagccaac aatatcgtca
      901 ccaggcacgg ggttacaaca cttagcaatc cgcactagga gaccagaagc accttcaata
      961 accactcccc cctcatgctt gaccttgagg gtttctttat tttcaacctt gacctcgcca
     1021 cctttgacaa gctcctctgc ctcagctttg gccttggcac gctcttcctc acggcgttcc
     1081 ttttcagtca gacggttaaa gacggtaatc gcaccgattt ccccaaaacc aatggccgca
     1141 aagagggagt cttctgtctt gtaactggtc ttttgcagaa cttgatccat gtggcgcttg
     1201 tccataaatt tatttgccac atagccattt tcttggaact gagccatcag catctcacga
     1261 cccttgttga cagacaattc cttatcttgg tttttaaaga actggcgaat cttattgcgc
     1321 gccttgctag tcttgaccat attgagccag tcacggctag gtccaaagga gttcgggttg
     1381 gcgataattt caacctgatc ccctgtcttt aacttggttg tcagtggaac catgcggcca
     1441 ttgaccttgg caccagttgc tttttcaccg accttggtat ggatttcgta ggcaaaatca
     1501 atcggtcctg aatctttggg aagggaacgg acagctccat ctggggtaaa aacgtaaatc
     1561 tcctcagcca aatagttttc cttaacagag tccacaaatt ccttagcatc atcagcctgg
     1621 tcttggagct ccatcatctc cttgatccag ttcattccaa tagctgattc cttgctgtta
     1681 acttgcccct ttataccttt cttataagcc cagtgagccg caaccccgta ctcagccacc
     1741 tcgtgcattt ccttggttcg aatctggaat tcaatcggcc cttttggtcc ataaacagtc
     1801 gtatggatag actgataacc attggccttg cggttggcga tatagtcttt gaagcgacct
     1861 ggcatcggtt tccaaaattc atgcacgtaa ccaagcatgg cataaacatc actttgggta
     1921 tctaaaatac aacgaatagc aatcagatca tagatttcct caaaccgttt tctcttgtcc
     1981 tgcattttgc ggaaaattga gtaaatatgc ttgggacgac cataaatctt ccctttcaag
     2041 tgacgttctg tcgtatactc ctctaatttt gtgactacct catccaccaa ggcctcacgc
     2101 tccctgcgct tttccttcat catatgggta atcttgtaaa actccgttgg attgagataa
     2161 cggaaagaca agtcttctaa ttcccatttg acactggaaa tccccaaacg atgggcaagc
     2221 ggggcataga tttccatggt ttctttggaa atacgctcct gcttgtcttt tcgaagatgt
     2281 ttcagggtcc gcatattgtg caagcggtca gacagtttga ccaaaataac gcggatgtcc
     2341 tcagacatgg ccatgagcat cttgcgatga ttttccgcta attgctcctc gatcgatttg
     2401 tactcgacct tgccaagctt ggtaactccg tcaacaatca tccgcacatc aggaccaaac
     2461 tctctttcca aatcgtccaa agtcgcatct gtatcttcca ccacatcatg caagaatcca
     2521 caagctactg ttacagcatc cagctttagc ttagctaaaa tacctgccac ttggataggg
     2581 tgaatgatat aaggctcgcc tgatttgcga tattgaccac tgtggcattc aacagcatag
     2641 accaaggcct tatggacaaa atgaacatcc tcttccgtta aatattcttt ggttaaagcg
     2701 acaacttctt cgcctgttaa attcacttct ttcggcatct ctactctcca attcttccta
     2761 ccattttatc acttttttaa gaatatgaaa actagattgg aacagaataa gaaaaaaata
     2821 attcaaaatt gcttgataat tctgaattat tggtccgtaa tatactacga agttagattt
     2881 taaacttagg tgatagaagg agagatagaa gaacggaaac catattgtaa cccaaagact
     2941 ttctgacttc cccaattcca ttgaagatac gaaagataaa cggtggaact cgtatcacat
     3001 acactggtac cttgactgga ttttggaatt aatactaaat gaaaatcaaa gagcaaacta
     3061 ggaaactagc cgcaggttac tcaaagcacc gctttgaggt tgcagataaa gttgacgcgg
     3121 tttgaagaga tttttgaaga gtataaaaat cctcaagata ctttcttcta tcctttagtt
     3181 tataaggaga atacctatga aaaaaactgc tatttctatc tttgctctcc taatgttagg
     3241 agtttgctgc ctgttcctat tcagccagca aagctataaa aaacagtcgt tcaatactat
     3301 gctaacgacc agaacctgcc cagtaggata acttatagtg aatatagcga caaatgagaa
     3361 gccaactacg gtagcactct aaacatcacg tctatcaaac aagctaatga cggagtttat
     3421 gcaacctatg aagggcaatt gacacctttc caatattgat aaattgataa ccagcctgtc
     3481 ttcatctagt catgctggtt tttaagttca ttttaaatcc ttacctattc tccctaactg
     3541 tgctatactt aatttatact caatgaaaat caaagagcaa actagaaagc tagccgcagg
     3601 ctgttcaaag cactgctttg aggttgcaga taaagttgac gcggtttgaa gagattttcg
     3661 aagagtatta gtacattctt tgagattgga gctagtatga aaatccataa aaccgtgaat
     3721 cctgttgcct atgaaaatac ctattatcta gaaggcgaaa agcacctcat cgtcgtcgat
     3781 cctggtagtc attgggaagc cattcgtcag acaatcgaga agatcaacaa accgatctgt
     3841 gctattctct tgacccacgc ccattatgac catatcatga gtctggactt ggttcgcgag
     3901 acgtttggca atcctcctgt ctatatcgca gagagcgaag ccagctggct ctacactcct
     3961 gtcgataatc tctccggtct ccctcgccac gatgatatgg cagatgtggt cacaaaacct
     4021 gcagaacaca cctttgtctt tcacgaagaa taccaactag aggaatttcg ttttaaggtt
     4081 ctaccgaccc cagggcactc tatcggtggt gtttccctag tctttcctga tgctcatcta
     4141 gtcttgacgg gagatgctct attccgcgaa actatcggac ggaccgacct tccgactggt
     4201 agcatggagc aactccttca tagtatccag acccaactct tcaccctacc aaactacgat
     4261 gtctatccag gacatggtcc agctactact atcgctcacg aaaaggcctt caatcccttt
     4321 ttctagcaag atgatgacaa tcgaaattta agtaaactat ccagcaaatc tttctattac
     4381 aaaaggcatc ctatcaaggt tttcacacat gattggatgc cttttttctg atgactagat
     4441 tttttgcatt accaaataat cacgcgctcc tctggtgaac gccacattcc gtctccttct
     4501 ttgacatcat aggttgtaaa gaaatcgtcg aagtttggta cttgcacatt gacacggagt
     4561 ttggctggtg cgtgcacatc gacgctagcc aaaagtttca taaattctgg tcgacctttc
     4621 atgcgccaga tgcgaccgaa gttgtagaag aactcttctg ctgagaagtc tgcttctctc
     4681 ttagctgctt caagcgctgc tgcgattcct cccaagtcag ccacgttttc tgatacagtc
     4741 aatttaccgt taatggttgc tccataagaa tcctgtccat caaattggtc aatgactttt
     4801 tgtgttttct ccttgaaggc agcatagtcg ctctctgtcc accaatcctt gaggctacca
     4861 ttttcgtcaa aggaagcccc gttagtatca aaggcgtggg aaatttcatg ggcaatcact
     4921 gccccaatac caccgtagtt agcagaagat gactgatgca agtcatagaa aggcgcctgt
     4981 aaaatggccg ctggaaagac aatcaggttc ttctgaggat tgtagtaggc attgaccata
     5041 tgagcaggca tgccccattc cttataatct acaggctggt tccacttact ccaactgtgc
     5101 ttgatttcca cacgcgcaaa ggctagagca ttctcaaaaa gactggcagt ttcattcact
     5161 accttatcct tgtaacgtgc aggcaattct tctggatagc caatataagg tttgatcaca
     5221 ttgagcttca cgatagcctg tttacaggtt tctggagtga gccagtcatt cttaagcaga
     5281 cgctccttat aaacatcaat catggttgcc acttttttct ccacatccgc cttggcttct
     5341 ggagagaact tctcacgggc gtaccaaaga cccagggctt gcttgaaagg ttcttgtgct
     5401 agatgataag ctgctttgac cttatctttt gcctctggaa ctccagaaag ggcacggctg
     5461 taggcaccag acaaaacacg gatatcctct gttaaatagc tggttgaaag attgacaaca
     5521 ctcaaaatca aggttgcttt aaggagagac caggcttcct cactgtagaa ttgctctgct
     5581 gcttgccaga aacgttcctc gtctacaata accttgtctg gtaattgccc aataactgct
     5641 ttgaagaagt catccaaagg tagggcaggc gcgaatttct tgaaatcttc gtaagaatat
     5701 ggatgataga gtttagcata ttctgaactt tcttcattag agagcaccac tgccgcaact
     5761 cggcggtcca attcaagtct tttttctagc aagtcttcaa tttcttcatc agagaaatca
     5821 taagccttga ggagatttgc gctgctttct ttccaaagag tcaagagctc ttcgcgctga
     5881 ggatgttctt ctgcatagta ggtcgtatct ggcaagattg tgcttggagc gctagcccat
     5941 agaacattga ttctagcatc cataaagtct ggcgatacac caaaaggaag gaagtttggt
     6001 tttcctgcaa gctcaaactc tgctagttta gctgtaaaat ccgcaaaagt ctccaattct
     6061 tggaattctt taaggagtgg taagacaggt gtgataccgt cagcttctct cttgtcaaaa
     6121 tcacgaacta ggcggtggta tttgacaaag ttttccaaga tagcatcctc aggcacttct
     6181 tcacctgcta accacttgtc tgttgtcgcc agcatcaggt cttcaatttc ctggtctaaa
     6241 tcaacaaaac ctcctgtttg agacttatct gctgggattt cagctgtctg ttgccattct
     6301 ccattgatag catcataaaa atcatcttga taacgtgtca tcttgttctc gctttcattt
     6361 gtatttgcat ttatcttaac aaaaatcact agggaatgca attaataacc atttaactat
     6421 tattttttag ctattataaa aaatctttcc atgataaaac gcataattcc aagtttttta
     6481 cacttgatac tatgcgtttt ataattt
//
LOCUS       DAVMAF010000092         6030 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMAF010000092 DAVMAF010000000
VERSION     DAVMAF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.093"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            70..312
                     /locus_tag="VYO97_001869"
     CDS             70..312
                     /locus_tag="VYO97_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1754620.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            314..568
                     /locus_tag="VYO97_001870"
     CDS             314..568
                     /locus_tag="VYO97_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1754621.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            615..1310
                     /locus_tag="VYO97_001871"
     CDS             615..1310
                     /locus_tag="VYO97_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1754622.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(1647..2633)
                     /locus_tag="VYO97_001872"
     CDS             complement(1647..2633)
                     /locus_tag="VYO97_001872"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1754623.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2827..5295
                     /gene="gyrA"
                     /locus_tag="VYO97_001873"
     CDS             2827..5295
                     /gene="gyrA"
                     /locus_tag="VYO97_001873"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1754624.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            5295..>6030
                     /gene="srtA"
                     /locus_tag="VYO97_001874"
     CDS             5295..>6030
                     /gene="srtA"
                     /locus_tag="VYO97_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1754625.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQ"
BASE COUNT         1902 a         1002 c         1312 g         1814 t
ORIGIN      
        1 ctttttatat tgactgaaca cctaaaaagt aatacaatgg tgttaccatt aaaaaaggga
       61 gcacaaaaga tgactactat tacattaaaa gtttctgaag ctgataaaac atttatgaaa
      121 gcaatggcta agtttgaagg agtttccctt tcggaactta ttcgcaccaa aactcttgaa
      181 gccctagaag atgaatacga tgctcgtgtg gcagatttag cctatcaaga gtatttagaa
      241 gacttggaaa aaggagttga acccattact tgggaagaaa tgatgcatga tttaggcttg
      301 aaggatgaat aatttgtata aattagttcc aacaagacgt tttatcaagc aattgaaaaa
      361 attggaccgt tatacgcaga agctaattac aaactattta caaaccaatg ttttggaaga
      421 cccaagacga cacggaaagg ctttggttgg taatcgcgtt ggtcaatggc gctatagaat
      481 tggtaattat cgagttatcg tacaaattgt agatgatgaa ttagtcgttg ctactctaga
      541 agttggtcat cggagagata tttattgaat taaagctgag taataacagt atagaaagta
      601 aggagagtaa aaatttgaag aaaaaatggt tttttgcaga ttattatgac acaacaatta
      661 ttttacttgc tttaatctct gttattctgg tattacttgg atttgctgag atgactgatt
      721 tggacaatcc gccttatagt attattgatt tagtaatctg gggtgttttt gtaattgatt
      781 atagttggcg cttttttatt actaaaagaa aatggcgctt tattcttgaa aatatttttg
      841 atttattgac cattctccct ttaaatgcta tttttacagt gtttcgatta ggacgtatct
      901 tccgcttagc aaggctgaca aaattactga aactgactcg tttacttaga ataattggac
      961 ttacaggtaa actagaaaga aaaattagta gattcttacg aacaaatgga ttgatttata
     1021 tcttgtatgt taatatcttt attgttctag tagggagttc gattttatcc gttgttgaag
     1081 aaaaatcctt ctcagatagt ctttggtggg ctcttgtgac agtaactact gttggttatg
     1141 gtgatattgt tccagtttcc ctttttggga aatggctagc agttttactg atgcttgtcg
     1201 gtattggaac aatagggatg ctaacaagtg ctttgacgaa cttttttgta aaggataatc
     1261 cagatgaaca gataaaactt gataaactca aagacgaatt atcgagctaa agaatattaa
     1321 tagagaaaca atctgaaaaa atcgaagaac tacattgaat gataaaagac ttagttgaaa
     1381 aaatatcata aaggtgagac aaaataaaac ttgattcgag gagaaatcaa gtaagtcttg
     1441 acacaataga acgcatatta tcaggtataa aaacttgata ctatgcgttt tatagagaaa
     1501 taaaataaaa tctgaacaaa tcaattagga atttaaagca ttttataaca acgttttaga
     1561 gtgatggggt gctatttcaa cttcaaccta ctataataca gaaaaaaaca actccctgat
     1621 gattcaagga gttgtctata gttaaattag tttttagaag cttcttggaa ttctggattt
     1681 ttccatgctt cgtcaataat agcttgcaat tctttagcag atgcttgcat tttttgagtt
     1741 tctgcatcgt tcaatggaat gtttactgga cgaacgatac catgtgcacc aacaacagct
     1801 ggttgaccga taaagacatt ctcaactccg tattgacctt cttggaatac tgaaagtgga
     1861 agtactgcgt tttcatcgtc aaggattgct ttagtgatac gagcaagggc tactgcgata
     1921 ccgtagtatg ttgcaccttt tttgttgatg attgtgtagg ctgcatcacg aacaccttcg
     1981 aacaattcaa tcaattcagc ttcttgaaca ttttgagtgt ctttaaggaa ttcttcaagg
     2041 tttacaccag cgatgttagc gtgtgaccaa acagcgaact cagagtcacc gtgttcaccc
     2101 atgatgtagg cgtgcactga acgagcatcc acatccaatt tttcagcaag tgcttgacgg
     2161 aaacgagctg agtcaagtga agtacctgaa ccgataacgc gttctttagg gaaaccagag
     2221 aatttccaag ttgagtaagt caaaacgtca actgggttag cagcaacaag gaagatacct
     2281 ttgaaaccag attcaacaac ttgagttacg attgatttgt tgatagcaag gtttttacct
     2341 acaaggtcaa gacgagtttc acctggtttt tgaggtgcac ctgcagtgat cacaacaagg
     2401 tcagcgtctg cacagtcaga gtattgagct gcatagattt ttttaggtga agtgaaggca
     2461 agggcgtgac taaggtcaag cgcatcacca acagcttttt catgcaattg tggaatttcg
     2521 ataattccaa gctcttgtgc aattccttgg ttaacaagtg caaaagcgta agatgaacct
     2581 acagcaccat caccgacaag gataactttt ttgtgttgtt tagttgaagt cattgtttta
     2641 aacatctcct taattttatt aggggatttt ccctagacaa cttcattcta tcacttttaa
     2701 aaaactttgt cacgaatatg ccttatagtt ctcgatgtaa acgttttagt ggtttagagg
     2761 ctgaaataga tgggaattta tggtataatg ttgttactta ctaattgtga aatgaggcat
     2821 ttattaatgc aggataaaaa tttagtgaat gtcaatctga caaaggagat gaaggcaagt
     2881 tttatcgact acgccatgag tgttatcgta gcgcgagctc ttcctgatgt tcgagatggc
     2941 ttaaaacctg ttcaccgtcg cattctctac ggaatgaatg aattgggtgt gaccccagac
     3001 aaaccccata aaaaatctgc tcgtattaca ggggatgtca tgggtaaata tcacccacac
     3061 ggggattcct ctatttatga agccatggtc cgtatggctc aatggtggag ctaccgttac
     3121 atgcttgtag atggtcatgg gaattttggt tccatggatg gagatagtgc tgccgctcaa
     3181 cgttataccg aggcacgtat gagcaagatt gctctggaaa tgcttcgtga tatcaacaaa
     3241 aatacagttg atttcgttga taactatgat gccaatgaac gggaaccctt ggtcttgcca
     3301 gcgcgttttc caaacctttt ggttaatgga gcaactggta tcgcggttgg gatggcaacc
     3361 aatattccac ctcataatct gggtgaaacc attgatgcag tgaagttggt catggataat
     3421 cctgaagtga ctaccaagga cttgatggaa gtcttgcctg gaccagattt tccaactggt
     3481 gctcttgtca tggggaaatc aggtatccat aaggcttatg aaacaggtaa aggttcgatt
     3541 gtcctacgtt ctcgtacaga gattgaaacg actaagactg gtcgtgagcg catcgttgta
     3601 acagaatttc cttacatggt caataaaacc aaggtgcatg agcatattgt tcgcttggtt
     3661 caggaaaaac gcattgaggg tatcacagca gtacgtgatg agtcaaaccg tgaaggtgtt
     3721 cgatttgtta ttgaagtcaa gcgcgacgcc tcagccaatg ttattctcaa taacctcttc
     3781 aaaatgaccc aaatgcaaac caattttggt ttcaatatgc tcgctatcca aaatggtata
     3841 ccgaaaattt tgtctcttcg tcagattttg gatgcttata tcgagcacca aaaagaagtg
     3901 gttgttcgtc gtacacgttt tgataaggaa aaagcggaag cgcgcgctca tatcttagaa
     3961 ggtctcttga ttgcgctaga ccatatcgac gaagtgattc gtatcatccg tgctagtgaa
     4021 acggatgcgg aagctcaagc tgagttgatg agcaagttta agctttctga acgtcaaagt
     4081 caagctatcc ttgatatgcg tcttcgtcgt ttgacaggtt tggaacgcga taagattcaa
     4141 tctgagtatg atgacctctt ggctctgatt gcggatttag cagatattct tgctaagcct
     4201 gaacgtgttt ctcaaattat caaagacgaa ttggatgaag ttaaacgtaa attttctgat
     4261 aaacgccgta cagagttgat ggttggacag atcttgagtc tcgaggatga ggacttgatt
     4321 gaagaatcgg atgtcttgat taccctttct aacagaggct acattaagcg tttggatcag
     4381 gacgagttca ctgctcaaaa acgtgggggt cgtggtgtcc aaggaacggg agtgaaagat
     4441 gatgactttg ttcgtgagtt agtgtcaact agcacccatg atcatctgct cttcttcaca
     4501 aacaagggac gtgtctatcg tcttaaaggt tatgaaattc ctgagtatgg tcggactgcc
     4561 aaagggctac cagtagtcaa tctcttgaaa ttggatgaag acgaaagtat tcagacggtt
     4621 atcaatgttg agtctgatcg cagtgatgat gcttatctct tctttacaac ccgtcacggt
     4681 attgtgaaga gaactagtgt taaggagttt gccaatattc gtcaaaatgg tctcaaagcg
     4741 ctgaatttaa aggatgaaga tgagttaatc aatgtcttgt tggcagaagg agatatggat
     4801 attatcattg gtaccaagtt tggttatgca gttcgcttta atcaatcagc cgttcgtggt
     4861 atgagccgta tcgccactgg tgtgaaaggg gttaaccttc gtgaaggaga cacagttgtt
     4921 ggtgccagct tgattactga tcaagatgag gttcttatta tcacagaaaa aggatatggt
     4981 aagcgtacag tcgctactga atacccaaca aaaggtcgtg gtggtaaggg aatgcagaca
     5041 gctaaaatta ccgaaaaaaa tggcttgctg gccggtctta tgactgttca aggggatgag
     5101 gatttgatga ttatcactga tacaggtgtc atgattcgaa ccaatcttgc caatatttca
     5161 caaacaggac gtgcaactat gggagttaaa gtaatgcgcc tggatcaaga tgctcagata
     5221 gtgactttca caacggttgc ggtggcagaa aaagaagaag ttgggacaga aaacgaaaca
     5281 gaaggtgaag cataatgtct caaaaaaata ataaaaagaa aaacaagcga aaaaatctgc
     5341 tgacaaatat cctagcagga tttctgatat tactgtcact ggctttgatt tttaatactc
     5401 aaattcgaaa tattttcata gtctggaata ccaataagta tcaagttagc caggtatcaa
     5461 aagaaaaatt agaagaaaat caggatacag aaggcaattt tgactttgat tctgtcaaag
     5521 ctatctcttc ggaagctgtt ctaacttctc aatgggatgc tcaaaaatta ccagttattg
     5581 ggggaattgc aattcctgaa ttggaaatga atttgccgat ttttaaagga cttgataatg
     5641 ttaatctctt ctacggagct ggtacaatga aacgcgagca agtaatggga gaaggaaatt
     5701 atagtctagc tagtcaccat atctttggtg ttgataatgc taataaaatg ttattttctc
     5761 ctttagataa tgctaaaaat ggcatgaaga tttatctaac cgataaaaat aaagtttata
     5821 cttatgaaat acgtgaagtc aaacgtgtga caccggatcg tgttgatgaa gttgatgata
     5881 gagatggggt taatgaaatc acattagtaa cctgtgaaga ccttgctgct acagaacgta
     5941 ttattgtcaa aggtgatttg aaagaaacaa aagattattc acaaacatct aatgaaatcc
     6001 taacagcttt caatcaacca tataaacaat
//
LOCUS       DAVMAF010000093         5770 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMAF010000093 DAVMAF010000000
VERSION     DAVMAF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.094"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            317..1834
                     /gene="malQ"
                     /locus_tag="VYO97_001875"
     CDS             317..1834
                     /gene="malQ"
                     /locus_tag="VYO97_001875"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1754626.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            1860..4118
                     /gene="glgP"
                     /locus_tag="VYO97_001876"
     CDS             1860..4118
                     /gene="glgP"
                     /locus_tag="VYO97_001876"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1754627.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTSTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(5163..5735)
                     /locus_tag="VYO97_001877"
     CDS             complement(5163..5735)
                     /locus_tag="VYO97_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754628.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNRRINF"
BASE COUNT         1734 a         1134 c         1134 g         1768 t
ORIGIN      
        1 ggaaatcagg aggggctacc tccctgtttc taatccctat ctattttcta tagacgtttg
       61 tgctttgttg catataaatt caagctccct aaggaggttt tgtactttgt agactgtctc
      121 gtgagagtca aactttagga ttagatagtt ttaagttatc tttgctaaaa tgagttagtc
      181 tccttaaaaa taatcaaaaa agttttttaa aaacgcttgc aattatgctt gaaaaggagt
      241 atacttataa gtaacgcaaa cgtttgcgtc tgcaaaatac gcaacgttcc attattttaa
      301 cacacgaggt gctattatga aaaaacgtca aagtggtgtg ttgatgcaca tctcttctct
      361 tccaggagct tacggaatcg gatcatttgg tcaaagtgct tacgacttcg ttgatttctt
      421 ggtccgtaca aaacaacgtt actggcaaat ccttccatta ggagcaacta gttacgggga
      481 ttctccttac caatctttct cagccttcgc aggaaacact cattttatcg atttagatat
      541 cttggtggag caaggtttgt tgggagcgag tgaccttgaa ggagttgact ttggtagcga
      601 tgcgtctgaa gttgactatg ctaaaatcta ctatgcacgt cgtcctcttt tagaaaaagc
      661 ggtgaaacgt ttctttgaag tcggagatgt taaagatttt gagaaatttg ctcaagacaa
      721 ccaatcatgg cttgagctct ttgctgagta tatggctatc aaagagcatt ttgacaatct
      781 tgcttggact gaatggccag atgcagatgc tcgtgctcgt aaagcttcag cacttgaaag
      841 ctatcgtgag caattggcag acaagttggt ttaccaccgt gtgactcaat acttcttctt
      901 ccaacaatgg ttgaaattga aagcttacgc taacgacaac cacattgaaa tcgttgggga
      961 catgccaatc tacgtagcgg aagattcaag tgatatgtgg gcaaatccac atctcttcaa
     1021 aacagatgtc aatggtaagg ctacttgtat cgcaggatgc ccaccagatg agttttctgt
     1081 aactggtcag ctttggggta acccaatcta tgactgggaa gcaatggaca aagacggcta
     1141 caaatggtgg attgaacgct tgcgtgaaag cttcaaaatc tacgatatcg ttcgtatcga
     1201 ccacttccgt ggcttcgaat cttattggga aatccctgct ggttccgata cagcagcacc
     1261 tggtgagtgg gtgaaaggtc caggctacaa gctttttgca gccgttaagg aagaacttgg
     1321 tgagctaaac atcatcgcag aagaccttgg cttcatgaca gatgaagtga tcgaattacg
     1381 tgaacgtact ggcttcccag gaatgaagat tcttcaattt gccttcaacc cagaagacga
     1441 aagcattgat agcccacact tggcacctgc taactcagtt atgtacacag gaacacacga
     1501 taacaatacg gttcttggtt ggtaccgtaa tgagattgat gatgcgactc gtgagtacat
     1561 ggctcgttac acgaaccgta aagaatacga aacagtggta cacgctatgc ttcgtacagt
     1621 attttcatca gttagcttta tggcaattgc aactatgcaa gatttactag aattggatga
     1681 ggcagctcgt atgaacttcc catctaccct tggtggaaac tggtcttggc gtatgactga
     1741 agatcaattg acaccagctg tcgaggaagg tttgcttgac ttgacaacaa tttatcgccg
     1801 aattaatgaa aatttggtag atttaaagaa ataagacaat aatcaggaga caactaaaca
     1861 tgttatcact acaagaattt gtacaaaatc gttacaataa aaccattgca gaatgtagca
     1921 atgaagagct ttaccttgct cttcttaact acagcaagct tgcaagcagc caaaaaccag
     1981 tcaacactgg taagaaaaaa gtttactaca tctcagctga gttcttgatt ggtaaactct
     2041 tgtcaaacaa cttgattaac cttggtcttt acgacgatgt taaaaaagaa cttgcagctg
     2101 caggtaaaga cttgatcgaa gttgaagaag ttgaattgga accatctctt ggtaatggtg
     2161 gtttgggacg tttggctgcc tgctttatcg actcaattgc tactcttggt ttgaatggtg
     2221 acggtgttgg tcttaactac cactttggtc ttttccaaca agttcttaaa aacaaccaac
     2281 aagaaacaat tccaaatgca tggttgacag agcaaaactg gttggttcgc tcaagccgta
     2341 gctaccaagt accatttgca gactttactt cgacatcaac tctttacgat attgatgtta
     2401 ctggttatga aacagcgact aaaaaccgct tgcgtttgtt tgacttggat tcagttgatt
     2461 cttctattat taaagatggt atcaactttg acaagacaga tatcgctcgc aacttgactc
     2521 tcttccttta cccagatgat agtgaccgtc aaggtgaatt gctccgtatc tttcaacaat
     2581 acttcatggt ttcaaacggt gcgcaattga tcatcgacga agcaatcgaa aaaggaagca
     2641 acttgcatga ccttgctgac tacgcagttg tccaaatcaa cgatactcac ccatcaatgg
     2701 tgattcctga attgattcgt cttttgactg cacgtggtat cgagcttgac gaagcaatct
     2761 caattgttcg tagcatgact gcctacacta accacacaat ccttgctgag gcgcttgaaa
     2821 aatggcctct tgaattcttg caagaagtgg ttcctcactt ggtaccaatc atcgaagaat
     2881 tggaccgtcg tgtgaaggca gagtacaaag atccagctgt tcaaatcatc gatgagagcg
     2941 gacgtgttca catggctcac atggatatcc actacggata cagtgttaac ggggttgcag
     3001 cacttcatac tgaaatcttg aaaaattctg agttgaaagc cttctacgac ctttacccag
     3061 aaaagttcaa caacaaaaca aacggtatca ctttccgtcg ttggcttatg catgctaacc
     3121 caagattgtc tcactacttg gatgagattc ttggagatgg ttggcaccat gaagcagatg
     3181 agcttgaaaa acttttgtct tatgaagaca aagcagctgt caaagaaaaa ttggaaagca
     3241 tcaaggctca caacaaacgt aaattggctc gtcacttgaa agaacaccaa ggtgtggaaa
     3301 tcaatccaaa ttctatcttt gatatccaaa tcaaacgtct tcacgagtac aaacgccaac
     3361 aaatgaacgc tttgtacgtg atccacaaat accttgacat caaagctggt aacatccctg
     3421 ctcgtccaat cacaatcttc tttggtggta aagcagctcc agcctacaca atcgctcaag
     3481 acattatcca tttaatcctt tgcatgtcag aagttattgc taacgatcca gcagtagctc
     3541 cacacttgca agtagttatg gttgaaaact acaacgttac tgcagcaagt ttccttatcc
     3601 cagcatgtga tatctcagaa caaatctcac ttgcttctaa agaagcttca ggtactggta
     3661 acatgaaatt catgttgaac ggagctttga cacttggtac tatggacgga gctaacgtgg
     3721 aaatcgctga gttggttggt gacgaaaaca tctacatctt tggtgaagat tcagaaactg
     3781 ttatcgacct ttacgcaaaa gcagcatata aatcaagcga attctacgct cgtgaagcta
     3841 tcaaaccatt ggttgacttc attgttagcg atgcagttct tgcagctgga aacaaagagc
     3901 gcttggaacg tctttacaat gaattgatca acaaagactg gttcatgact cttcttgact
     3961 tggaagacta catcaaagtc aaagagcaaa tgcttgctga ctacgaagac cgtgacgcat
     4021 ggttggataa agtcatcgtt aacatttcta aagcaggatt cttctcatct gaccgtacaa
     4081 tcgctcagta taacgaagac atctggcact tgaactaata ctcttcgaaa atctcttcaa
     4141 accacgtcag ctttatctgc aacctcaaag cggtgctttg agcaacctgc ggctagcttc
     4201 ctagtttgct ctttgatttt cattgagtat aagatacaaa tttatactaa tacattttgt
     4261 aaaaaagcga gtttcgattg aaattcgctt ttttaaatgc tgtggatttg ggtcaatctt
     4321 gtctaaaaat agggaaatcc tagatacagt gaaggcttta aatgctggtt tttactgtct
     4381 tcagccttat attttttcgt agttggttac ctcatatctc ttgtattcgc ttacataaag
     4441 tattataata taattgtagg aaagaaggtg tttttatgat atacacactt aaattggtgt
     4501 tgtttattac ctttcttgta ataagcttgt tacctgataa gatttttgga aaaaataaaa
     4561 aaatttggaa aatagttttt gcaatattga cggcagtggc agcattgtca tttatgtact
     4621 aagttatttt aagaatgtag ggaaataaac cctacattct ttttagtttt ttctgttttc
     4681 taaattctat ttatccaagc gcttcaacat ttcttgcttc ttcgcttcaa gttctgcacg
     4741 cttttcttcg atttcggcat gttttttctc gagttcagaa caacttgcac cattgctaaa
     4801 ttcttttcgc catcaggaga tagggtgagt cgacgtgtct attactcacc caaagcagtc
     4861 ctacaaagca ggaattttct gttacttttt tggaaatagt aacgtttata cagctttgac
     4921 acttcgtatc aaagcgccaa acacactccg agggatttac agaaagcaga aaaggaatga
     4981 tctggtataa gatcattcct tttctctctt tttctttaag taattatata caatgtacga
     5041 cgaagtcgtc attgcaatgc tgatccacca cctaaaggga actttaaaca acattgataa
     5101 gataaagaat ataaacaacg aaaatacgtt atacccaatt aattttattg tatatctcat
     5161 gattaaaagt taatccttct gttgttagga atggcatcat ttttatccca taattgtgct
     5221 aaataagtcc ccggtgataa taaattcata gcgaattcta aagcaacatc atttacaaac
     5281 caactaccta gatatctaga aattgctgaa cgaatagcac tttttgctgc atgttttcct
     5341 tttactttaa ttagatttgc aaggcctgca gtagttcctc ctaatgctaa agctattgca
     5401 gtatctaata gagcacccat ttgattaact gtaatacctt gccaagctgc tctaaatgga
     5461 gagtatgtag gtgggattgt ataatcgcct tgtaattgtc ggttaattac ttctttgatc
     5521 cattgttgtg agacgtctgg atgaaaagat tggatttcgt ttgcaagtgt attgatttgt
     5581 tcttctgtta gagaagtgac aggttgaagt tccatatttg tttcaatttg tgatacttgt
     5641 tcagaagcgt atacagctga aacacttgga atcgctgata caattaacac aattgacgtc
     5701 aaaaaaaccg aaataaattt cattaatttg ttcatgaact tttctccttt ttatttgtat
     5761 ctgcttacat
//
LOCUS       DAVMAF010000094         5758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMAF010000094 DAVMAF010000000
VERSION     DAVMAF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.095"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            480..1523
                     /locus_tag="VYO97_001878"
     CDS             480..1523
                     /locus_tag="VYO97_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1754629.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1690..3777
                     /gene="topA"
                     /locus_tag="VYO97_001879"
     CDS             1690..3777
                     /gene="topA"
                     /locus_tag="VYO97_001879"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1754630.1"
                     /translation="MATVTKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3998..4357
                     /locus_tag="VYO97_001880"
     CDS             3998..4357
                     /locus_tag="VYO97_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1754631.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4450..5082
                     /locus_tag="VYO97_001881"
     CDS             4450..5082
                     /locus_tag="VYO97_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1754632.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            5126..>5758
                     /locus_tag="VYO97_001882"
     CDS             5126..>5758
                     /locus_tag="VYO97_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1754633.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKS"
BASE COUNT         1788 a          979 c         1257 g         1734 t
ORIGIN      
        1 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataaa aaatggtctg aaatagatga tgatactttt
      241 cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatatttg
      481 tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat gaagaattag
      541 aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc tctatcggat
      601 taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct ttgatgacac
      661 ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta agaaaatctt
      721 ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt tatttttatc
      781 tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg actatttggg
      841 atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg aaaaaagaga
      901 ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct ctttgtacag
      961 taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct tacctatttc
     1021 tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg atgatggtta
     1081 agaaacatta ttttaaagat aatgaagaag actctaaaat gcgtaggatt ttgcttctag
     1141 ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg agagaaacgt
     1201 tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggcat aatattttga
     1261 aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga aattatttga
     1321 cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta agtgatgttt
     1381 ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagaa ctggtggagt
     1441 atttcttcca gtatatcaag gataaggaag caaaagaaaa ggaaaaagct aataagtttt
     1501 atacagagtc tgaggagcaa taatttcttg agaatagctg gtttttctcg tgagtcttct
     1561 atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat tatagtagct
     1621 aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac tttgaaaagg
     1681 tgtgatactg tggctacggt aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc
     1741 atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag aaactacaag
     1801 gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt
     1861 gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct tatcaatgac
     1921 ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa
     1981 ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa tgatgccaac
     2041 cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa agaacctcgt
     2101 aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga tcgcttggta
     2161 gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc
     2221 gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa tgccttccag
     2281 ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct
     2341 tccttctatg gagtagatgg taaaaagatg aaactgacca gcaatgacga agtcaaggaa
     2401 gtcttgtctc gtctgactag taaagacttt tcagtagatc aggtggataa gaaagagcgc
     2461 aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc tgccaataaa
     2521 atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga aggaattaat
     2581 atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac tcgtatcagt
     2641 cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag taagtattct
     2701 aagcacggta gcaaggtcaa aaacgcatca ggtgctcagg atgcccatga ggctattcgt
     2761 ccgtcaagtg tctttaatac accagaaagc atcgctaagt atctggacaa ggatcagctc
     2821 aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc ggccgttttt
     2881 gataccatgg ctgttaaatt gtctcaaaaa ggggttcaat ttgctgccaa tggtagtcag
     2941 gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa gatgttaccg
     3001 gacatggttg ttggagatgt ggtcaaacag gtcaatagca aaccagagca acatttcacc
     3061 caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga aaatggggtt
     3121 ggacgtccat caacctacgc gccaaccatt gaaaccattc agaaacgtta ttatgttcgc
     3181 ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa gctcatcgtt
     3241 gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg taaactggat
     3301 gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta caaaccattc
     3361 tctaaagaag ttgctaaggc tgaagaagaa atggaaaaaa tccagattaa ggatgaacca
     3421 gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt
     3481 aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat cgtgaaagag
     3541 attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt
     3601 aatcgcctat tctatggttg caatcgctat ccagaatgtg aatttacctc ttgggacaag
     3661 cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa agtccgtggt
     3721 ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat taaataagag
     3781 gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa atacatcatt
     3841 ttatgcgaaa ctagaagaag attattttat actcaatgag aatcaaagag caaattagaa
     3901 agctagtcgc aggttgctca aagcactact ttgaggttga agatggaagc tgacctggtt
     3961 tgaagagatt ttcgaagagt attaactagg agaagttatg cgtcttatct atctaattat
     4021 tggtttttta tcgctggcct tggctattgt tggggttgtt ttacccttgt tacctacaac
     4081 acctttcctt ttgttgtcta ttgcttgttt ctccagaagt tccaagcgat tcgaagactg
     4141 gctttatcat accaagctct atcaaacata tgtagctgat tttcgcgaga ctaagtctat
     4201 tacgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg gaatttctat
     4261 ttattttgca cctcttttac cagtcaaaat cggtctgggt gctttgacca tctttatcac
     4321 ttattatctt ttcaaggtca ttccagacaa agaatagtta aaacagtagt tatttgcctt
     4381 gataaaattg aaagcatatt cataacaata tgatataata aatttgaagt aatattcaag
     4441 gagaatcaaa tgatttacga attttgtgct gaaaatgtga ctttacttga aaaagcgatg
     4501 caggctggag ctcgtcggat tgaactctgt gataatctag cagttggtgg gacaacaccc
     4561 agctatggag tgactaaggc agcggttgaa ctggcagcta actacgatac aaccatcatg
     4621 accatgattc ggccacgtgg tggtgacttt gtctataatg acctagaaat tgctatcatg
     4681 ctagaagaca tttgtttgac tgctcaggct ggaagtcaag gggttgtatt tggagcttta
     4741 actgctgata aaaagttgga taagcctaat ctggaaaagt taattgctgc atcaaaagga
     4801 atggaaattg tctttcacat ggcctttgat gaactaagtg atgaagatca accggaagct
     4861 attgactggc tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg tgtgtctggc
     4921 gactccttag aaaaacgttt tgttcactat cacagaattt tggagtacgc taaaggtaaa
     4981 attgaaattc taccaggtgg ggggattgac cttgacaacc gtcaaacctt tattgaccag
     5041 gtgggggtaa cacaattgca tggtactaag gttgtttttt aaaaaataga aaggaactgc
     5101 tagccttggg tagcagtttt cacttatgtt tgaaattttt aaatcctatc aatttaatca
     5161 agaaaaggct catgattatg gttttataga aaatagcgaa gtctggacat atagttgcca
     5221 gattttgcaa ggtgactttg tcatgactgt gtccatcact gctgataatg tgaactttca
     5281 agtctttgac caagagactg gtgacctcta tcctcacgtt catatggaaa gcatgagggg
     5341 aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga ttcggaaggc
     5401 ttgttttgat gtgcaagatt ttatctgtca tcagactaag cgtatcatga ctcaagttca
     5461 ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata cagctgtatt
     5521 gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg aaaatctctt ggaataagct
     5581 ggaaaagggc agagaaggac aagtggaagc agtcaacctc aagcatgacc aagtagctaa
     5641 tttgctttca caaaagggga tttatccagc cttccatatg agcaagcgct actggattag
     5701 tgtgtccctt gatgatactt tatcagatga agaagtactg gaattgatag aaaaaagt
//
LOCUS       DAVMAF010000095         5656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMAF010000095 DAVMAF010000000
VERSION     DAVMAF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.096"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            102..266
                     /locus_tag="VYO97_001883"
     CDS             102..266
                     /locus_tag="VYO97_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1754634.1"
                     /translation="MGIIANVFAGLVGASAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            350..916
                     /gene="amaP"
                     /locus_tag="VYO97_001884"
     CDS             350..916
                     /gene="amaP"
                     /locus_tag="VYO97_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1754635.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            928..1098
                     /locus_tag="VYO97_001885"
     CDS             928..1098
                     /locus_tag="VYO97_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1754636.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1137..1745
                     /locus_tag="VYO97_001886"
     CDS             1137..1745
                     /locus_tag="VYO97_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1754637.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1776..1979
                     /locus_tag="VYO97_001887"
     CDS             1776..1979
                     /locus_tag="VYO97_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1754638.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2089..2361)
                     /locus_tag="VYO97_001888"
                     /pseudo
     CDS             complement(2089..2361)
                     /locus_tag="VYO97_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2506..2952)
                     /locus_tag="VYO97_001889"
     CDS             complement(2506..2952)
                     /locus_tag="VYO97_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754639.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            3022..3681
                     /locus_tag="VYO97_001890"
     CDS             3022..3681
                     /locus_tag="VYO97_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1754640.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYTLFELGLGLLFLLYSWEWLSLGQVVLITAG
                     VTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(3733..4884)
                     /locus_tag="VYO97_001891"
     CDS             complement(3733..4884)
                     /locus_tag="VYO97_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1754641.1"
                     /translation="MFNGQVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(4899..5616)
                     /locus_tag="VYO97_001892"
                     /pseudo
     CDS             complement(4899..5616)
                     /locus_tag="VYO97_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1746 a         1033 c          955 g         1922 t
ORIGIN      
        1 ttgatattaa aataaaaagg agggtaggca gtgttgtgat caattattgc tggaggtctt
       61 attggtctct tggcaggtaa aatcactaaa aaagtagttc tatgggaatc atcgcaaatg
      121 tattcgctgg tttagtcggg gcatctgcag gacaatctct tttaggtagt tggggtccat
      181 caatcgctgg aatggctttg ctcccatcta ttgtaggtgc agcgattgtg attactgtag
      241 tgtcattctt tacaggtaga aagtaaactt ttcgccagta aagttagcaa actattttta
      301 aatcaatgac gggaaaaata gtttaaatgt taaatcgaaa ggattgtata tgtcaaaagc
      361 aaagaaaata tgtttcatta ttttctgtat tttaatcttg acaattttcc ttcctgtttt
      421 gatagattat catcaagtta gtgatctagg tattcatcta cttagctgga gacagaactc
      481 cgtagttgaa ttctatcttg ctagatatgt cttttggggg acagtggttc tatcaacttt
      541 agttttatta tccattttag ttgtgatgtt ttatcctaaa cgttacttgg aaatccaact
      601 tgaaactaaa aacgatacat taaaattaaa gaattcggca atcgaaggtt ttgttagaag
      661 tttggtgagt gatcatagat tgatcaagaa cccaactgtt catgtaaatt tacgaaaaaa
      721 taaatgtttc gttcatgtag aaggtaaaat tcttccttca gacaacatcg ctgacagatg
      781 ccaaataatt caaaatgaaa taactaatgg attgaagcag ttttttggta ttgagcgtca
      841 agtaaaactt gaagttgcag taaaaaatta ccaaccaaaa cctcaaaaca aaaagactgt
      901 tagtcgtgtg aagtaaggaa gtaaaaaatg gaatggctta aacaatatcg atatccaatt
      961 atcgctggtc tcataggcgt atttctggct tgtttgattg tctcctttgg cttcttcaaa
     1021 acaatatttg tattgatttt aggagcactg ggagttgcag ctggattata tatcgaaaaa
     1081 aactatatag ataaataaaa aaataaaaat tactaattta attaaaggag tttcatatgt
     1141 caaacgaaaa aaacacaaac actaacgtag aaaagaaaga tgctactgtt gtagctcacg
     1201 aaatcaaagg ggaacttact tacgaagata aagttatcca aaaaatcatt ggtctttcac
     1261 tagaaaacgt ttcaggtctt ttgggaatcg atggtggttt cttctcaaat cttaaagaaa
     1321 aaatcgttaa cagcgatgac gtaacaagtg gtgttaacgt agaagttggt aaaacacaag
     1381 ttgcagttga cttaaacgtt attgttgagt accaaaaaaa tgttccagct ttatattcag
     1441 aaatcagaga aatcgtatct tcagaagttg ctaaaatgac tgacttggaa attgttgaaa
     1501 tcaacgtaaa cgttgtcgac atcaaaacta aagaacagca tgaagcagac tcagtaagcc
     1561 ttcaagatcg cgtatctgac gttgctgaat caacaggaga attcacttca gaacaattcg
     1621 aaaaagctaa atctggtctt ggatctggtt tctcaactgt tcaagaaaaa gttagcgaag
     1681 gtgtagaagc tgttaaaggt gcagcaaatg gtgtagtatc tcacgaaaac actcgtgtaa
     1741 actaagataa aataaatata acaggagaaa ttatcatgtc agtagaagaa aaattaaatc
     1801 aagctaaagg ttctattaaa gaaggtgttg ggaaagccat cggtgatgaa aaaatggaaa
     1861 aagaaggagc agctgaaaaa gttgtttcta aagtaaaaga agttgccgaa gacgctaaag
     1921 acgctgtaga aggtgctgta gaaggtgtta aaaacatgtt gagtggcgac gataaataag
     1981 gttaaaagtt actttatctt tttggtaaca ttagtcaaaa gagtctgagt caagatgatt
     2041 ctcagaaaac aaaaagctag agattcccaa ttgcggaact ctagcttttt aattttgcct
     2101 ctttctctta ttatatttca gcaggttgtt ggccatgagt atgaatccca tgtcaattct
     2161 cacttgacgc ttacctctca gatgacatct cttataaccc aaacaaacct ttatctgccc
     2221 aaagacagat ttcatatcaa tcttacgttt agcgaaaatt tgtctaccct tggaagataa
     2281 aagtgcctga tattctttag tttttaaaca ctggtaacgt tcattcatat acagtccctt
     2341 ttgaggggct gattcaggtt cataatcgca gtcaacattg atttcaaggc tgtttgcttt
     2401 ctatctcccc tttttcataa tgtatagtaa aatgaaataa taacaggacg aatcgatcgg
     2461 gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa
     2521 tctactatat tttcgtacag gtgcttcaac catttgaacg atttcaaatc cttctttttg
     2581 gtaaagattc tgagctcttt gatttgcctc gaagacattt agagaaatac tatctatatc
     2641 ttcattttca aaggccaaac taacaaattt ccttaaagcc tggctaccta agccttgctc
     2701 ctgtttctgg gggttgataa aaaatctccc gatatgaaga ttgctgtctt ctagcctgat
     2761 tttctggatt agtcccacaa actcttgttc atcaaagatt gaaaagactc cttccaaggc
     2821 ttgaagggtc agtagaaaag gaatccttgg tcccatccat tgttcttgaa aggatttgcc
     2881 tagggagttg gaccactggc atacaaattg agcgttttct gtgctcacct tttcttcaaa
     2941 acgaattgtc atcttttcct caccatctta tctatgtttc tccattatac tatttctccc
     3001 attttttacg aatagataag tatgattgat ttttattttt ttctcgtcgg gagcattctc
     3061 gcttcctttc ttggtttggt cattgaccgt tttccagagc aatccattat cagttcagcc
     3121 agtcactgcg attcctgtca gactcgcttg cgtcccttag atttgattcc gattctctca
     3181 caggtcttca atcgcttttg ctgtcgctac tgcaaggtcc gctatcctgt ctggtatacc
     3241 ctctttgaac taggcttagg actcctcttt ctgctttact cttgggaatg gctctccttg
     3301 gggcaagtcg tcctaatcac cgctggtgtg accttgagca tctatgactt tcgccatcag
     3361 gaatatccct tactggtctg gatgactttc cacctaatcc taatagcttc ctctggctgg
     3421 aatctggtca tggtctcctt ccttgctctt ggaattttgg ctcattttat cgatatccgc
     3481 atgggcgcag gggatttcct ctttttagct tcttgtgctc tcgtctttag cataacggag
     3541 ttactgatct tgattcagtt tgcttctgcg acgggtatcc tagcctttct cctgcaaaag
     3601 aaaaaggaaa gacttccttt cgtgcctttc ctcttacttg ctacttgttt gattattttt
     3661 ggtaagctac tgcttgtctg ataaaatcca atttctgcca tatgtccttc atgaaattat
     3721 ttcacagtta aattataaat tatttctttt gtacaaaggg atgatgttat caaatcgatc
     3781 tgttcttcta tcttcttgat actgatcaaa aaatttcatt tcgactgaaa atatttcgct
     3841 tataaactgt aaacgaatac tttgtttaga cattatagtc gctagactga ctagatgatt
     3901 actcaaaacg acgtccagaa tactctttac tttgcttggt tttttaacaa aaatttgatc
     3961 atccaaaggt tcaatcattt tgtaaccttt ttgcgcaatt tgacgataaa agtaagaatg
     4021 ttgctttgga gtcaataatc ctaacttaaa agctcgatac tctaaagcct gtatcgaaac
     4081 attcaaatct gacttcaata aaatataact atcaggattg ctgacacgct tgccaaccct
     4141 ctcttcaaat ttgactaaaa actcttcttt tggcaataaa aaacatgatg caaaataatt
     4201 tgcttcttgc tccaaacgat cgccatcttc attcatatct ttatatttat gtaaaagaat
     4261 atgtcctagc tcatgagcta agtcaaaatt tcgacgtaca gatgatttat tcgttcccaa
     4321 cacaatataa ggtcttccca attttgacca tgcgctataa gcatcagctt ggccattaat
     4381 taatcgttcc acgatataga tgcctgaacg ttctaattta taaagcaaat catgattatc
     4441 ttttgaaata cctaattttt ccctggcata aagagccaat tcctcaatgg attctccctt
     4501 atgataagat tcactcacta cattacttaa gtcatgaatt gtaatattag gtataattac
     4561 aaaactttca aaataatcaa tcagactatc taccttatgt aaatacatag tttgaatatc
     4621 tattgttttc cgtgttgcta ggtctgcatt tctaaaggca attacagaag aatcaaatcg
     4681 aatgctctct tcttcctgtt caaaataagt taaatcaaca tgaaattggt tggccaaatg
     4741 cattttggtt gataatttag gtttcgtttc gttggactca aactgccaaa tggcttgttc
     4801 cgttaaatta attctctgag ctaattctgc tctacttaaa ccatttaaca gccgtaattc
     4861 tttcaatacc tgaccattaa acatttacat actccttact acttttgacc ttcttgtttt
     4921 tctattcttg gaataatttc aaaatcttct gtttccgata attctgaaaa attaggaata
     4981 tcttgatatt tagcttcttc gaaatggtac gaacttgatt gaatatactc agacaaatct
     5041 tgaatcaagt gtaactgacc agttcgtgca tcaggcataa caacatctac agatataata
     5101 ttgttttctg agtccgcctc ataagttaaa atcataaatt tttcgatatt cgaattttta
     5161 gtagcttgtt caatttcttg aatcatttca tcagaaacta actccatctg aattggaaag
     5221 gaatgactat cttcatcatt tttgtaggaa gaatgttgat taagataaag tgtattcaaa
     5281 taagtagcca ctcttatttt tttgtaccaa aggaaattgg ttcgtaagtc gcttcttacc
     5341 ctttataatt aacaatactt tcccatattt ttctgtattt gtttcaaatt ctaaatatcc
     5401 ccaagtctgt cctgctaatt gtaatttata ctcaaacaaa tctgctgatg caaatgcagt
     5461 atcaatatga ttaggtcgcg tccatgcata accattcgac actatcattg tctctctttt
     5521 ttctagacgt tcatctacat aatctttttg ccctttcatc aaagtatcta caattttttg
     5581 tgcctcaagc gaatcaaaga gatcctgatt caacataatt cttcctcctc caaatacttt
     5641 ttaatgaatt atatca
//
LOCUS       DAVMAF010000096         5482 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMAF010000096 DAVMAF010000000
VERSION     DAVMAF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.097"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..1457
                     /gene="rlrA"
                     /locus_tag="VYO97_001893"
     CDS             <1..1457
                     /gene="rlrA"
                     /locus_tag="VYO97_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEV1754642.1"
                     /translation="IELDELSTLTSLQSKSLLSILQELQETFEEELTFNLDTQQVQLI
                     EHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEFTQKEYISIATGYRVRQKCGL
                     LLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYDLNDGSMDWVTHMIVQSNSQL
                     SHELLEITPDEYVHFSILVALTWKRREFPLEFPESKEFEKLKNLFMYPILMEHCQTYL
                     EPHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKTHTIQLILQHTRGKHLLSKFK
                     NILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYYEHYGIESDKPLYHISKAIVQ
                     EWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIFIILNNQADVNLIKSIILRNF
                     TDKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPYVKKQYPKGKHHFLTIALDLH
                     VSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
     gene            1722..2984
                     /locus_tag="VYO97_001894"
     CDS             1722..2984
                     /locus_tag="VYO97_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1754643.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGAFPSLIRLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGY
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(3042..5366)
                     /gene="pflB"
                     /locus_tag="VYO97_001895"
     CDS             complement(3042..5366)
                     /gene="pflB"
                     /locus_tag="VYO97_001895"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1754644.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1662 a         1129 c          993 g         1698 t
ORIGIN      
        1 ctattgaact agatgaactg tctactttaa cttcattaca atctaaaagt ctcctaagca
       61 ttttgcagga attacaagaa acattcgagg aagagttgac atttaattta gatacgcaac
      121 aagttcaatt aatcgaacac cattctcatc aaacgaatta ttacttccat cagctttata
      181 accaatcaac tattttaaaa atactccgct tctttctact acaaggtaat caaagcttta
      241 acgaatttac acaaaaagag tatatttcca tcgcaacagg ctaccgcgta cgacaaaagt
      301 gtggcttgtt actacgttca gtaggtttgg accttgtgaa aaatcaagta gttggtcctg
      361 aatatcgcat tcgtttttta attgcactcc tccagtttca ttttggtatt gagatttatg
      421 atttgaatga tggaagtatg gattgggtca cacatatgat tgtgcagtct aattctcaac
      481 tcagtcatga actgttagaa atcacacctg acgagtatgt tcattttagc atactagtgg
      541 cactcacttg gaaacgtcgg gaatttccac ttgagttccc tgagtccaaa gagtttgaaa
      601 aattaaaaaa cctctttatg tatccgattt tgatggaaca ttgccaaact tacttagagc
      661 cacatgccaa catgactttt actcaggaag aactggatta tatctttttg gtttattgtt
      721 ctgctaattc ttcgttttcc aaggacaaat ggaatcaaga aaaaaagact catactattc
      781 aactgattct tcagcataca agaggaaagc atctactatc caagttcaag aatatcttag
      841 gaaatgacat ttcgaattct ctttcttttt taacagcact gactttctta acaagaacgt
      901 ttttgtttgg tttacaaaat ctagttccct attataatta ttacgaacat tatgggattg
      961 aaagcgacaa gccactgtat catatttcga aagcaattgt tcaagagtgg atgactgagc
     1021 agaagattga aggagttatc gaccagcatc ggctgtacct tttctcactc tacttgacag
     1081 aaaccatctt ttcctcacta cctgctatcc cgatttttat cattttaaat aatcaagcag
     1141 atgtaaactt aataaagtcc attattttac ggaattttac tgacaaagtt gcctctgtta
     1201 caggatacaa tattctaatt agtcctcctc cttcagagga gcatttgaca gagccattga
     1261 ttattattac aacgaaagaa tacctgcctt acgttaaaaa gcagtatccc aaagggaaac
     1321 atcacttttt gacgattgcc ttagatttgc atgttagcca acagcgtctc atctatcaga
     1381 caattgtaga tatccgaaaa gaggcttttg acaaacgggt cgcaatgatt gctaaaaagg
     1441 ctcactattt gttataagtt catcgtactg tctacacaca aaaaggggct tcctaagcca
     1501 tctctattct tatccccata tttcccagtt ttgggtaaat ctgaacagaa aaacacttct
     1561 gctctctagt ttctgctagt gaacaaaagt gttttttctg gctctataat ttctgtagtg
     1621 ggtaaatccc actgtggaga ttatagggct ttttgagtat caaaaaagtc ccataagact
     1681 tataatgaaa agcgcctaaa caactcatta gaaagtatct tatggaacac atcaatcata
     1741 ccacattact catcggaata aaagacaaaa acatcacctt aaataaagcc attcagcacg
     1801 atacccatat cgaagtcttc gctacacttg attatcaccc acctaaatgt aaacactgta
     1861 aaggaaaaca aatcaaatac gacttccaaa agccttctaa aatccctttt atcgagattg
     1921 gtgctttccc tagcctcatt cgtttgaaaa agagacgatt tcaatgcaag tcctgtcgga
     1981 aagtcactgt agctgaaaca actctcgttc agaaaaattg ccaaatctcg gaaatggtga
     2041 gacagaaaat tgcccaactc ctactcaaca gagaggctct tacacatatc gcttctaaat
     2101 tagccatctc tacctctacc tctaccgtct atcgtaagct caagcaattt catttccaag
     2161 aggattacac cactttgcct gaaatcctct cctgggatga attctcctac cagaagggga
     2221 aattggcttt cattgctcag gatttcaaca ctaagaaaat catgaccatt cttgataaca
     2281 gacgtcaaac aaccatccga aatcatttct tcaagtactc gaaagaagct agaaaaaaag
     2341 ttaaagtcgt cactgttgat atgtctggaa gttatatccc tctcattaag aaattatttc
     2401 ccaatgctaa aattgttctc gatcgtttcc acattgtcca acacatgagc agggctctta
     2461 atcagactag aatcaacatc atgaagcaat ttgatgataa atctctggaa tacagagctc
     2521 ttaaatatta ctggaaattt atcctaaaag atagtcggaa actctctctt aagcctttct
     2581 atgctagaac tttcagagag accttaactc ctagggagtg tctgaagaaa atctttactc
     2641 tagtacctga acttaaagat tactatgacc tgtatcaact actcctattt catctacaag
     2701 agaagaatac tgaccagttt tggggcttaa ttcaagacac gttacctcat ctcaaccgca
     2761 cctttaaaac cactttgagc acatttattc gctataaaaa ctacatcact aacgccattg
     2821 aattgcctta ttctaacgct aaacttgagg ccactaacaa actcatcaaa gacatcaagc
     2881 gcaatgcctt tggttatcgg aactttgaaa acttcaaaaa acggattttc atcgctctga
     2941 acatcaaaaa agaaaggaca aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
     3001 tacagttgac aaagagccct ttttattatt caagaacttg attagctcaa tgcatccaag
     3061 gcgtcatcca ttgaaagaac ttcgtggaag acacgttgtg tcaattcagt tttttgttct
     3121 ggagtgaggt atttagtatt tacacagtat ccagagatac gtacgataac gtcttcacct
     3181 gacatgattt tttcgtaaac atcgttcaag tccataacgt tcaagttaac gtgttgtcca
     3241 ccgttttcga agtaaccatc aaggattgtt accaagttat caacttgttc atcacgagtc
     3301 ttaccaagag cgcgaggtga tacttgtgta gtcaatgaga taccgtcagc tgcataacta
     3361 aagtcaaggc tagaaagtga gttcaagttt tgcaaccaac cacctttagc tttgttagat
     3421 gggttagcac ctggtgagaa gaattcaagt ttagacaagt tcacagaacc atcttcgttg
     3481 aggtatacac ctttgtgaac tggtgagtta ccagtttgtt tagagtaagc aacgttagat
     3541 gtgattgtca aaagtgatac tgtagcttct gcgtctttgt atagtttgtg gctacgtaga
     3601 cgagttgtgt aagcttcgat caaccattct gccaattcgt ttgaacgtgg gtcatcttca
     3661 ccccagcgtg ggtagtcacc gattgtttcg taatcgtaga tgtagccatc ttcgtcacgg
     3721 attggtttaa ctgtagcgta tttgatagct gacaatgtat caacagtgtt agcaaatcca
     3781 cagataccga atcccatgtt ggcacgttgt ttagttggca agaaggccat ttgaacagct
     3841 tcgtagttgt acctatcagt catgtagtgg atgatgttca aggcatctac gtaagtgtca
     3901 gtcaaccagt caagagattt ttcaaagttc gctttaactg attcaaattc aagaacttcg
     3961 tcacggattg gttcgatatc aaatactttg tagtctttgt gaacatcgtc gtaaccacca
     4021 ttcaaaccag taagaagggc tttaagaacg tttacacgag caccgaagta ctggatgttg
     4081 tggcgttgtt cttcattttc tggatcaagt ggagacacac agcatgagat acagctcatt
     4141 tcaccatatc cgtctttagc cattgttgtt acaccttcgt attggataga agagtgtttg
     4201 tggctcatgt gcatacagta gcgacggaag ttgtatggca atttgtcagt ccaaagaact
     4261 gtcaagtttg gttctggtga gttaccgatg ttgtcaagag tgttcaagaa acggtagtcc
     4321 atcttagtaa cacggtgacg accgtcgtta cccataccag ccatagaagt tgtgataaag
     4381 gttgggtcac ctgagtacaa ttggtcataa gcttttgtac gagcaaattt aactgtacga
     4441 agtttcataa cgaaatcatc aacgaattct tggatttctg attcagtaaa tgtaccacga
     4501 gcaaggtcac gttctgcaaa gatgtccaat acgattggta cacgacctag agatgtagca
     4561 gcaccgttaa tcacacggca gacagccatg aaagcaatgt taacccattg gattgcttct
     4621 ttcacgttca tcgctggttt gcgaacatca accccgtaaa ggtcacccag gcgaacaact
     4681 tgttgcaatg cttggtattg aaggtttact tcttcacgaa gacggattgt ttcttcatcg
     4741 atttctttga ttgcattcca gtcatttact ttttcttgca tcaagtagtc tgcaccgtaa
     4801 agagcaagac gtgcgtaaac accgatgata cgtccgcgtg agtatgcatc tggaagacca
     4861 gttacagtgt gtgcgtgacg agcgcgacga atatttgaag tgtaggcacg gaaaataccg
     4921 tcgttaactg ttgttacata tttagtgaag atttcgtgaa cagctgggtc tggttcgtat
     4981 ccattttctt tcaaagtagt ttcagccata cggataccac cttttggcat gaagttcaat
     5041 ttgaagagtt catcgttttg gataccgaag ataacttcat tttctttgtc gataaatcca
     5101 gcagggatat cagcgataga tgttggacga gtgtccattg ggaaacgagt ttcttcgtag
     5161 tgtgctttag tttcttctac aattttcttg atgtgaagtg aacgctctgt tggtcctgca
     5221 aggaagcttt cgtctccatc ataaggtgtg tagttagctt gtacaaatcg tgatacactt
     5281 gctttttctt tccaatctac gcctttgaag ccttcccaag ctttgtcaaa aatatcttgt
     5341 gcttcaacaa ctgtcttaac aaccatatta atgtcctctt ttttctagta acagtcatct
     5401 gttacattca taagacaagt ataccatata gtaaccgatt tcaacaagtg aaaattccct
     5461 atttttacac tttcttttct aa
//
LOCUS       DAVMAF010000097         5109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMAF010000097 DAVMAF010000000
VERSION     DAVMAF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.098"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(344..1072)
                     /locus_tag="VYO97_001896"
     CDS             complement(344..1072)
                     /locus_tag="VYO97_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1754645.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1230..2639
                     /locus_tag="VYO97_001897"
     CDS             1230..2639
                     /locus_tag="VYO97_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1754646.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2657..3952
                     /locus_tag="VYO97_001898"
     CDS             2657..3952
                     /locus_tag="VYO97_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1754647.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3957..4265
                     /locus_tag="VYO97_001899"
     CDS             3957..4265
                     /locus_tag="VYO97_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1754648.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNTPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4262..4570
                     /locus_tag="VYO97_001900"
     CDS             4262..4570
                     /locus_tag="VYO97_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1754649.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1719 a          863 c          932 g         1595 t
ORIGIN      
        1 atttctagca attttttaga agtcacagtc tactattcta acctcagtat actatatgtg
       61 ttacaagaaa agactcaaaa acttctcgtc agtgtaagaa tatttgaaac actatttaaa
      121 actaaataac tataagaagt agatcaggtg aacaatttca gtacctactc tataagctat
      181 acgaagatat ttttcaacat gatggataaa acgatcactc ttttaaagct atcttattta
      241 gaaaagagaa accatttaag catagatggg accttcttgc cctgttttaa atttttctta
      301 aactagagtc tgtaaaagat tggcttcatt ttcacatttt ctcctaggag gaatgtcgta
      361 atgcaaaaga attaaattca aataaatcat agcgatgacg actaatcgag tactcaaacg
      421 gagttccgtt atccaaataa gccacttgtt ctacttcaaa taccggttcc gttggaagga
      481 gctgcaagta atgttgctca ttttctgaac tagaagaagc tcgcaagatt tttgtagcac
      541 tggcaatatg caatcctaac tttccttcaa tgtaattgta aatcgatttt tgtaaaatat
      601 cttcagtaat acctggaata acatcggtac tcatataagt ttgttccaga acataaggtt
      661 taccgtcaat aatacgcagg cggtaaatat tataaactgg actctgcaaa gcgacctgta
      721 gtttttctgc taaaaattca tttgcaaatt ctaatttgaa gtgaataatc ctcgagtcaa
      781 ttgtagaatg agcatcttca gatatttttg tcagtccccg gatatctctt tctggaacga
      841 ttaattttct ttttgagctg ccacgagaga gaacaaaggt tccctgccca cgttttctaa
      901 aaattaagcc ctcagaaact aacaagtcta aagctttttt gatcgtcatt cgactacagg
      961 caaactgttt agctaatgtc atttcatcag gcaattgttc gttcatggta tatttattac
     1021 aaagaatctt ttcacgaatc tcattgtaaa taaataaata ttttgtactc atttaatctg
     1081 gttctctcta taatattcta atagtagtat aacaaagttc aagaaatgag gcaaacacaa
     1141 gatatgtatc aacaattttt aaaaatgtct agacaaattt taaaattatg ctatacttta
     1201 tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa gttagtattt cctaatctgt
     1261 tttggtgggg agctgcttct agcggacctc agacagaagg tcaatatgga aaagtacatg
     1321 aaaatgtgat ggactactgg ttcaaaacgc atccagaaga ttttttcgat aatgtcggac
     1381 ctcttgtagc cagtaacttt tttcatactt acaccgaaga tttccacttg atgaaggaaa
     1441 ttggagttaa ttctttccgc acttccatcc aatggagtcg actcatcaag aatttagaga
     1501 caggtgagcc tgatccaaaa ggtattgctt tctacaatgc catcattgaa gaagctaaaa
     1561 agaaccagat ggatcttgtg atgaatttac atcattttga tttaccagtg gaacttcttc
     1621 aaaaatacgg tggttgggaa agcaaacatg tagtggagtt attcgtgaag tttgccaaga
     1681 ctgctttcac atgctttgga gataaggttc attactggac aactttcaat gagccaatgg
     1741 tcattccaga agcaggatac ttatatgctt tccattatcc aaatctaaaa ggaaagggaa
     1801 aagaggccgt acaagtcatc tataatctaa accttgctag tgcaaaagtg attcaactat
     1861 atcgctcatt aggacttgat ggaaagattg ggattatttt aaacttgaca cctgcttatc
     1921 caagaagtaa ttctccagaa gacttagaag caagtcgatt tacagaagac ttctttaaca
     1981 aagtcttctt gaatccagct gttaaaggaa ctttcccaga aagattggta aaacagctag
     2041 agagagatgg cgtgttatgg agtcataccg aaaaagagct tcaactgatg aaatcaaata
     2101 cggttgattt tcttggagta aactactacc atccaaaacg tgttcaagca caagcaaatc
     2161 ctgaggaata tcagacgccc tggatgccag accaatactt caaagagtat gaatggccgg
     2221 agcgtcgcat gaatccatat cgtggttggg aaatttttcc gaaagccatt tatgatattg
     2281 ctatgattgt gaaggaagaa tatgataata ttccatggtt tatcagtgaa aacggaatgg
     2341 gtgttgaaaa cgaagcacgg tttatcgatg aaaatggagt tatcgatgac gtgtatcgta
     2401 ttgaatttta tgaagaacat ttaagatggc tacataaagc cattgaagag ggaagtcact
     2461 gttttggata ccacgcttgg accgcatttg attgctggtc ttggaataat gcatataaga
     2521 atcgttacgg atttatctcc gttgatttag aaacgcaaaa gagaaccatc aagagctcag
     2581 gaagatggta tcgcaaagta agtgacaata acggttttga agtagaaatt gaggagtaaa
     2641 gagaggagat attcagatga aaaactcaaa atttatagat caatttgcca cctttgctgg
     2701 taaactaggg aaccaaattc atttaaaaac cctaagagat gcattcgtaa cagtaatgcc
     2761 attatatatt ttggcaggtt taatcgttct tttgaacaac acggtattta agtggatttt
     2821 ccaaggggat acattaacaa aattccaata ttggggaata acaattgcaa acggtacttt
     2881 aagtatttca ggtatgatta ttgctgtaat ggttggttat ttcttagcta aaaacagaga
     2941 tttcgaaaac ccgttagcag catcaatgct atcattagtt tctttaattg tgatgatgcc
     3001 aaatacagtt tctgtagttc ctgacggagc aaaagatgcg gtaaacattt caggtgttct
     3061 ttcattcaac aacacaggta caggcgcaat gttcgccggg gttatcgtag cgattattgc
     3121 aacagaatta ttcattgaat tatcaaacgt taaagcttta caaatgaacc ttggtgaaaa
     3181 tattccacca gctgttagta gatcatttag cgtattactt ccagtcatga ccgtcatctc
     3241 cttatttggg gttgtttcag cattattatt caatataact ggaatgaact taatctcaat
     3301 cattacaatc tttattcaag aaccaattcg tcatattggt acaagcttaa tcggggtcat
     3361 tattatttac tctttaggaa atatgttatg gctatttggt attcaccaag cagttattta
     3421 cagtgccatc ctagaaccat tactattaat taacattact gaaaacatca ctgcagcaaa
     3481 taatggacaa gccattccac acatcatcaa cttatcacaa atacaaacat tcgctttaat
     3541 gggtggtagt ggatctacat tatgtttatt aatagcaaca ttcttagtga gtcgcaatgc
     3601 tgtttctaaa aacgtggcta aattatcttt tggacctggt atcttcaata tcaatgaacc
     3661 agtattattc ggttacccaa tcgtttataa catttcatta gctattccat ttatcacagt
     3721 tccagtcctt ggtattttaa tcagctactt agcaacagtt acaggattca tgagtcctgc
     3781 atttatacaa gttccttgga ctacaccagt attcttaaat gcatggttag caacagcagg
     3841 ggacgtgaga gcagttctag ttcaattcat catctttgca cttggagttc ttctatacat
     3901 tccatttatc aaagttaatg acaaagttgt tgaacaagaa atggaaggtt aagattatga
     3961 aacaaatttt attagtatgt aatgcgggaa tgtcgacaag tatgcttgtt aaaaaaatgc
     4021 aacaaagtgc gactgaacgt gggattgaaa tttctatcca agcaaaatca atgacagaag
     4081 ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg gccacaaatt cgttatgagt
     4141 tacttgctgt gaaagaaatt gcaggaaata cccctgtaga taccattgat atgcgggatt
     4201 acggaatgat gaatggagca aaagttctgg aacaagcact tgcatggata ggagaaataa
     4261 gatgacagaa gaaatggaat atatctgttt tcagctcatt gccaatagtg gagcagccaa
     4321 atcatccttt attgaagcca tccaactagc taaggcggga aatttaaaag aagcgaaaat
     4381 taaagttgaa gaagcggaag actccttagt agaagcgcac aagattcact ctaatctcat
     4441 acaaaaagaa gctactggag aaaaaatagg attctccttg ctatttatgc atgcagaaga
     4501 ccaaatggct tcaacagaga ttattcagtt gctatctaaa gaattcatcg atctctatca
     4561 aaataaatag gaacactgtc attcccatct gtctttatta atgacgacta cattgtagga
     4621 acctccaaaa gttggacaaa atgattgaaa ggattggatc ctgcattaca caagatccaa
     4681 tccttttctt ttactctagt ttttataata tgttgaatct agaatagtcc actgtagttt
     4741 ctaaaaaatt gctggaaatg gatgttaagc tccaatttat ttgtttatat cttatttcag
     4801 tttactatac tagcttatta ttctagtgaa aaattgaatg agccactatt tatttaatat
     4861 gtatgatgag ggaatgactc aaaaacttct tgtcgatata agaatatctg agatagtatt
     4921 ttaaaacgaa aggactataa aagctcattt ccttcgcact tattttatag agatctatca
     4981 ttatactctt tagaaatcaa aaagcaacac accttgtcat cgttttatcg taaatctgtt
     5041 aatgaggggg ctagtcttat ctgcaatctc aaatcagtgt tttgagctga atggcactcg
     5101 ttttctagt
//
LOCUS       DAVMAF010000098         5046 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVMAF010000098 DAVMAF010000000
VERSION     DAVMAF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.099"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            879..2138
                     /locus_tag="VYO97_001901"
     CDS             879..2138
                     /locus_tag="VYO97_001901"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1754650.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2154..2705
                     /locus_tag="VYO97_001902"
     CDS             2154..2705
                     /locus_tag="VYO97_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754651.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2698..3975
                     /locus_tag="VYO97_001903"
     CDS             2698..3975
                     /locus_tag="VYO97_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1754652.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLDGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3991..4851
                     /locus_tag="VYO97_001904"
     CDS             3991..4851
                     /locus_tag="VYO97_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1754653.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDITKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1532 a          824 c         1164 g         1526 t
ORIGIN      
        1 caagcctgac tatcgtgagg tagcggatta aaatggtctg gggatagacc gttttaagtc
       61 tgacgctgga aataagaatt gtcagaagaa ggaatagcga aatcgtggct ctacgaacaa
      121 gaacgtgata ataaggtgta tatagcggat aagggggcca aaagcactaa agtccaaaaa
      181 ggtagtcgta acctatatgc gtaaatcacg agagtaattg aattcgtact aagattttct
      241 attttcactg taacctttta acgtcctcat atcttctata cacgaggaaa gatgtacgac
      301 ttatcccgtg aggtctatca ctataaagag aaaacgacag atagaagcga tcctgagtca
      361 cggttatctt taaaatagat tgtctgatag gatgctagcc tgaaaacgtc ttgttcatga
      421 aatataggga cggtatgtat aaaacgcttc tgtgaactga gagaaggggg agaagttctt
      481 gataaaattt agttgaacag ccgtattccg atacttagat aagagatcta gtcttagctc
      541 ctactcggtt tcagaggata aaaaaggggc aatagcgatt cgagaaagat tatactcttc
      601 gaaaatctct tcaaatcacg tcaatatcgc cttgccgtac gtgtaggata ctgactacgt
      661 cagttccatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt
      721 gatctttgat ttttattgag tattagtaat tcagttacta actcgtcaac tctgatttat
      781 ccaataaaat tgaaaaggat ggaaaaaagg ataaatttat gatatacttt attttgaaga
      841 ccttattaga aatcttgaaa gagtattgaa aacttagaat gagaaaaatt gttatcaatg
      901 gtggattacc actgcaaggt gaaattacta ttagtggtgc taaaaatagt gttgtggcct
      961 taattccagc tattatattg gctgatgatg tggtgacttt ggattgtgtt ccagatattt
     1021 cggatgtagc cagtcttgtc gaaatcatgg aattgatggg agctactgtt aagcgttatg
     1081 acgatgtctt ggagattgat ccaagaggtg ttcaaaatat tccaatgcct tatggtaaaa
     1141 ttaacagtct tcgtgcatct tactattttt atgggagcct cttaggccgt tttggtgaag
     1201 cgacagttgg tctaccggga ggatgtgatc ttggtcctcg tccgattgac ttacacctta
     1261 aggcgtttga agctatgggt gccactgcta gctacgaggg agataacatg aagttatctg
     1321 ctaaagatac aggacttcat ggtgcaagta tttacatgga tacggttagt gtgggagcaa
     1381 cgattaatac gatgattgct gcggttaaag caaatggtcg tactattatt gaaaatgcag
     1441 cccgtgaacc tgagattatt gatgtagcta ctctcttgaa taatatgggt gcccatatcc
     1501 gtggggcagg aactaatatc atcattattg atggtgttga aagattacat gggacacgtc
     1561 atcaggtgat tccagaccgc attgaagctg gaacatatat atctttagct gctgcagttg
     1621 gtaaaggaat tcgtataaat aatgttcttt acgaacacct ggaagggttt attgctaagt
     1681 tggaagaaat gggagtgaga atgactgtat ctgaagacag catttttgtc gaggaacagt
     1741 ctaatttgaa agcaatcaat attaagacag ctccttaccc aggctttgca actgatttgc
     1801 aacaaccgct tacccctctt ttactaagag cgaatggtcg tggtacaatt gtcgatacga
     1861 tttacgaaaa acgtgtaaat catgtttttg aactagcaaa gatggatgcg gatatttcga
     1921 caacaaatgg tcatattttg tacacgggtg gacgtgattt acgtggggcc agtgttaaag
     1981 cgaccgactt aagagctggg gctgcactag tcattgctgg gcttatggct gaaggtaaaa
     2041 ctgaaattac caatatcgag tttatcttac gtggttattc tgatattatc gaaaaattac
     2101 gtaatttagg agcggatatt agacttgttg aggattaaac cgtagaggtg tttatgaata
     2161 tttggaccaa attagcaatg ttttcttttt ttgaaacgga tcgcttgtat ttgcgtcctt
     2221 tcttttttag tgatagtcag gacttccgcg agatagcttc aaatccagaa aatcttcaat
     2281 ttattttccc aacgcaggca agtctggaag aaagtcaata tgcactggcc aattacttta
     2341 tgaagtcccc tttgggagtg tgggcaattt gtgaccagaa aaatcaacaa atgattggtt
     2401 ctattaaatt tgagaagtta gatgaaatca aaaaagaagc tgagcttggc tattttttga
     2461 gaaaagatgc ttggtcgcaa ggatttatga cagaggttgt tagaaaaatt tgtcagcttt
     2521 cttttgagga atttggctta aaacaattat ctatcattac ccaccttgaa aataaagcta
     2581 gccaaagagt tgctcttaag tctggattta gtttgttccg tcagtttaag ggaagtgatc
     2641 gttacacaag aaaaatgcgg gattatcttg aatttcggta tgtaaaagga gagttcaatg
     2701 agtaagcatc aggaaattct aagctatttg gaggaattac cagtaggtaa aagggtcagt
     2761 gttcgtagca tttcgaatca tctaggagtt agtgatggaa cagcctatcg ggctattaaa
     2821 gaagctgaaa accgtggaat tgtggagacc cgtcctagaa gtggaacaat tcgtgttaaa
     2881 tcccagaaag ttgctataga gagattaacg tttgctgaaa ttgcagaagt gacttcttct
     2941 gaggttctgg atgggcaaga aggtttagag agagaattta gtaagttttc aattggtgcc
     3001 atgactgaac aaaatatctt gtcttacctt catgatgggg ggctcttgat tgtcggagac
     3061 cgaacccgta ttcagttgct agccttggaa aatgaaaatg cagttctggt tacaggggga
     3121 tttcaggttc atgatgatgt gcttaaactg gccaatcaaa aagggattcc tgttctaaga
     3181 agtaagcatg atacctttac cgtcgcgacc atgatcaata aagccttgtc aaatgtccaa
     3241 atcaagactg atattctgac agttgagaaa ctttatcgcc ctagtcatga gtatggtttt
     3301 ctgagagaga cagatacagt taaagattat ttggacttgg ttcgtaagaa tcgtagcagc
     3361 cgtttccctg ttatcaatca acatcaggtc gttgttggcg ttgtaaccat gagagacgct
     3421 ggtgataaat caccaagcac gacaattgat aaggttatgt ctcgtagtct atttttggtt
     3481 ggattatcga caaatattgc caatgtgagt caacggatga tcgcagaaga ctttgaaatg
     3541 gtaccagttg ttcgaagcaa tcaaactttg cttggcgttg tgacgcgacg agatgtcatg
     3601 gagaagatga gccgttccca agtttcggct ctaccaactt tttctgagca gattggacaa
     3661 aagctctctt atcaccatga tgaagtagtt attacagtgg aaccctttat gctagaaaaa
     3721 aatggagttt tggctaatgg tgtattggca gaaattctga cccacatgac ccaagattta
     3781 gttgttaata gtggtcgcaa tctcattatc gagcagatgc tgatctactt tttgcaggct
     3841 gttcagatag atgatatatt gcgcattcag gcacggatta ttcatcatac gagacggtca
     3901 gctataattg attacgatat ttatcatggt caccagattg tttcaaaagc aaatgtgact
     3961 gttaaaatta attagaaact aggagaaaag atgataacat taaaatcagc tcgtgaaatc
     4021 gaagctatgg acaaggctgg tgattttcta gcaagtattc atataggctt acgtgatttg
     4081 attaaaccag gcgtagatat gtgggaagtt gaagaatatg tccgccgtcg ttgtaaagaa
     4141 gaaaatttcc ttccacttca gattggggtt gacggtgcca tgatggacta tccttatgct
     4201 acctgttgct ctcttaacga tgaagtggct cacgctttcc ctcgtcatta tatcttgaaa
     4261 gatggtgatt tgctcaaagt tgatatggtt ttgggaggtc ccattgctaa atccgacctg
     4321 aatgtctcaa aattaaactt caacaatgtt gaacaaatga aaaaatacac tcagagctat
     4381 tctggtggtt tagcagactc atgttgggct tatgctgttg gtacaccgtc cgaagaagtc
     4441 aaaaacttga tggatataac caaagaagct atgtacaagg gtattgagca agctgttgtt
     4501 ggaaatcgta tcggtgatat cggtgcggct attcaagaat acgctgaaag tcgtggttac
     4561 ggtgtagtgc gtgatttggt tggtcatggt gttggcccaa ctatgcacga agaaccaatg
     4621 gttcctaact atggtattgc aggtcgtgga ctccgtcttc gtgaaggaat ggtcttaacc
     4681 attgaaccaa tgatcaatac aggcgattgg gaaattgata cagatatgaa aactggttgg
     4741 gcgcataaga ccattgacgg tggattgtca tgtcagtatg aacaccaatt tgtcattacg
     4801 aaagatggac ctgttatctt gactagccaa ggtgaagaag gaacttatta ataaaaagtg
     4861 aaaagactac tggaagttta ttttgataaa aaatccagta gatcttttca taataaaacg
     4921 cattgtatca agtgttagga gctgatatca tgcgtttttc tgcttttaag attttttcca
     4981 actctgtttg taagcgcatc ataacaaagg gtctaggatt cagggctctc ctcctatata
     5041 ctatta
//
LOCUS       DAVMAF010000099         4966 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVMAF010000099 DAVMAF010000000
VERSION     DAVMAF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.100"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            351..1223
                     /locus_tag="VYO97_001905"
     CDS             351..1223
                     /locus_tag="VYO97_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1754654.1"
                     /translation="MKKSRKLATLGICSALFLGLTACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1372..1713)
                     /locus_tag="VYO97_001906"
                     /pseudo
     CDS             complement(1372..1713)
                     /locus_tag="VYO97_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1856..2167
                     /locus_tag="VYO97_001907"
     CDS             1856..2167
                     /locus_tag="VYO97_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754655.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETAEKILALVPQDLIKQI
                     PFFVRKHATTRTIKRILIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            2171..2887
                     /locus_tag="VYO97_001908"
     CDS             2171..2887
                     /locus_tag="VYO97_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1754656.1"
                     /translation="MKKYFIGGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(3017..3832)
                     /locus_tag="VYO97_001909"
     CDS             complement(3017..3832)
                     /locus_tag="VYO97_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1754657.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(3829..4734)
                     /locus_tag="VYO97_001910"
     CDS             complement(3829..4734)
                     /locus_tag="VYO97_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1754658.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         1655 a          852 c         1007 g         1452 t
ORIGIN      
        1 tctaggaatt aatttgcctt tcctaataga gttgttcata tcttatttca atctactata
       61 tttttcacct ctaacaatcg caatctcttc tttatccatg aataaaaacg atttctattt
      121 ttgtaagtaa agcatcacac gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa
      181 aaaatttgtt gacagagcac gaaacgccta ctataattaa atcaagttta aggaaataac
      241 aaaaaaacaa atcggaggga aagacgatga acacattaaa tgagaaagta atcaatatct
      301 gtaaagcagt agttaaagaa actttaatcc aagacattta ggagaaagaa atgaagaaat
      361 caagaaaatt agccacatta ggaatctgtt cagctttgtt tttaggcttg actgcttgtc
      421 aacaacaaca tgctacttct gaggggacga atcaaaggca aagcagttca gcgaaagttc
      481 catggaaagc ttcatacacc aacctaaaca accaggtaag tacagaagag gtcaaatctc
      541 tcttatcagc tcacttggat ccaaatagtg ttgatgcatt ttttaatctc gttaatgact
      601 ataataccat tgtcggctca actggcttat caggagattt cacttccttt actcacaccg
      661 aatacgatgt tgagaaaatc agtcatctct ggaatcaaaa gaagggcgat ttcgttggga
      721 ccaactgccg tatcaatagt tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg
      781 aaaagaatga ccagttgctt ttcctagata atgatgcgat tgataaagga aaggtctttg
      841 attcacaaga taaggaagag tttgatattc tattttcgag agttccaact gaggcaacta
      901 cagatgtcaa ggttcacgct gaaaagatgg aaacattctt ctcacaattt caattcaatg
      961 aaaaagctcg aatgctgtct gtagtcttgc acgacaatct ggatggcgag tatctgtttg
     1021 taggccacgt tggggtctta gtacctgctg atgacggttt cttatttgta gagaaattga
     1081 ctttcgaaga gccctaccaa gcgattaaat ttgctagtaa ggaagattgc tacaagtatt
     1141 tgggcaccaa gtatgcggat tatacaggcg agggactggc taagcctttt atcatggata
     1201 atgataagtg ggttaaactt taatagtgtt atgatatgac agtaatctta ggatttttat
     1261 ttctattttt gatgagttgg tgggttattt caatcgttaa tagccagaaa ccacgcaaaa
     1321 atgaaacatt tattggctat gtccaaagat acgatattga tgggaatggc tatcaatcat
     1381 taccgtgtcc tcagagctga gaggagttct tgaaatcgta acaccacttt gaacaagagt
     1441 tacttcaacc cattggctcc gacggattaa gttgctttcg tgaataccaa aatcagccgc
     1501 aatttgttca taagtgcggt attctcgcac atattgaaga gtggctataa gaaggtcttc
     1561 taggcttaat ttaggttttc gtccaccttt tgcatgttta agttgataag ctgtttttaa
     1621 tacagctaac atctcttcaa aagtcgtgcg ctgaacacca acaagacatt tcaatcgtgc
     1681 atcagttagt tgtttacttg cttcatcatt catagaacta ttataccatg ttttgtttcg
     1741 caggaaatct aatgctaaaa tcttatcagt gagaagattt cttaaagttt attctttgta
     1801 gtttcatctg tattagtaca taatttaacc tagagaaaat aggaggacta atcgaatgga
     1861 attaaaagat tttacagaaa aagaacagga aatgattaag aaaaggctta caatgtctaa
     1921 tattagtgac aaagaaactg ctgagaagat tctggcgcta gtaccacaag acttgattaa
     1981 gcaaatcccg ttttttgtca gaaaacatgc tacaacacgt acgattaaac gcattttaat
     2041 tgaataccct gaactctacg ctgtagctca aacaagtggt gagattccag aaaaagaacg
     2101 cgaagaattg cgtcagatta tcacaactat ctttgaacaa aagatgaata agcatagtat
     2161 taagtagaga atgaaaaaat attttattgg cggtttggga agcaatgcct atcatagcaa
     2221 ggattttttt caagaactag attcgcaggt ctattttcta aatccatatg aaaagcatct
     2281 tcgagatgaa acagaattga aatcatggtt taaaaatgag attgtagagg aagaatctat
     2341 ctgtctgata ggccattctc ttggaggaga tttagctcgt tatttcgcat cggaatttga
     2401 agaagtaaag aaactgattc ttttggatgg tggctatcta gatttagata agattttacc
     2461 tttggataca gagttagagg aaactaaaaa ttatatcaaa tctcaaattg tttcggactt
     2521 agctcttctt atttctaaag agaagtccga agcaaagcat tggtcagaaa atatggagaa
     2581 agctgtaaga cagtcctatc actggaatgt tgagtataat agatatgagt tggctataaa
     2641 ttatgaaaat atagaagcga tactccgcct acggagaaaa atacaagctt ttaagagaga
     2701 agtgggagat accttgttta ttagtcctcg ctatcctaat ggagctacat ggagagagga
     2761 agccctaaaa gaattgccag actattttaa tactattttt ttagaaaact ttggccatga
     2821 gctttatact caagcaccta aagaaatcgc tagtctaatg aatgagtggc tcgcttattt
     2881 tctatgagct acttgtattg ctggaaatgg tataagatga aaatctacaa gtcactaatt
     2941 ttcatataac aaaaaaacga acaggctctt tttctgttat tcagaagaga acttgttcgt
     3001 ttttatcttg attttcttaa aaagctctat atacataggg attttctggt ttatcgactt
     3061 tggtaaagct gtcgatttcc aaggttggaa ggttttgttt gagttcttta tggtagtgat
     3121 tgaccagttt tcctttggct gtaatccagg tgttgttttc atactgccgg gtattgccct
     3181 tggtcagcaa tccatagaca ccagaatctg cgatacagtg gataatgccg aatcggaaca
     3241 gaaattgact attggcatga ctggggtcgt tatagacaaa gcctgtaaac tggattgtct
     3301 tgccctcaaa ttcatctgga tagtcgtaga tagcctccat gacttccata tagttttcat
     3361 tagtgatctg aatactatct tgggataagt atttatccgc cgccgttcgc atttcctttt
     3421 cataggctga ttttgaaaaa taagaactag tatctggttt caaatattgg cttgtagtcc
     3481 cttcacttgt ctgaatagct agatccgttc cttccgatag ggggaaatga taacctttag
     3541 cagaaacggt ctgagaatcc aagctaacag ttgggaaagt taagccgatg acaagtggaa
     3601 tagccagaag agaaatactc gtcatcttgg ccaatcggct gttcagatga ctgtgggttt
     3661 tgacttgctt catccagata tacaattgaa caattgccaa gataaaggaa agcaccatgg
     3721 agatataggc cagataggaa tagtgcatgt tgatgtactg gtttagtttg cccgacagat
     3781 ggaggtaaat agtcagttca aaatagccag ctaaaactaa aaatcgaatc ataggatgac
     3841 tccaatcaag agagaatagg ctaaaacgac cagagtcaca atcgtaatga agtgactaat
     3901 aaatcgtgct ttcaagtaat ttttcatcat gagaatattt ttgatatcca gcattgggcc
     3961 aatgacgaga aaggccagaa ctggtgccaa accgaaactc gagagtagag aagcacctat
     4021 aaaggcatcc gcctcactac agagcgaaag aagaaaggct aaaagcatca agagcaggat
     4081 agcaaaaaga ggagtcgcac tgatagaggt cagaatccga gtcggaacgt agacctgtat
     4141 tatagaagca aagaggcagc caaataccaa ataacgcccc gtatcaaaaa attcatcaat
     4201 ggcctgcaca aagacttgaa aaactttttt tgcagaactc aagtaagaaa aatcatgctc
     4261 atgacaagcc agacgatttt ctttctgaat cggttcttgc cagaaaaatc ctagaaaaat
     4321 tcctagtatt acagccacaa gaatggaacc cagagctcgt aatagggcga catggaagga
     4381 gttgccaaag gcagaatagg tcgcaaaaag aacaatggga ttgataacag gtgctgtcac
     4441 aagaaaagga acggccgtgt aacttggaac ctttttttcc agaaaacgat tgatgatggg
     4501 gacgattcca cattcacagg aagggaaaag tataccgaca aaggtcccaa aaaagattct
     4561 cccccaacga tttcgaggga gaaaatgata aaccttgtca ggtgtgatat aaacttcaat
     4621 cagccctgag acaatgcttc ctatcagaac gaagggaagg gcttcaatga taatggagag
     4681 aaaaatggct ccagtttgaa gtacactaga tgggagttgt tgaaaagcag tcatctagtt
     4741 tttcttttct gctttggctt ttttctcttt gtcatcaggg aaagtgactt gtttcaaatc
     4801 agggagttta gcaaactctt caaacatctt gtccaagtcg tctcgttttg taaatttgac
     4861 agccataggt gtacctcgat tctaaattct acctctatta tactattatt ttgcggaaaa
     4921 gggaaaagtt aaaatttcaa attgagtagc taagagataa tatagc
//
LOCUS       DAVMAF010000100         4937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMAF010000100 DAVMAF010000000
VERSION     DAVMAF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.101"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(86..1909)
                     /gene="dnaK"
                     /locus_tag="VYO97_001911"
     CDS             complement(86..1909)
                     /gene="dnaK"
                     /locus_tag="VYO97_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1754659.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAESDKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2389..2913)
                     /gene="grpE"
                     /locus_tag="VYO97_001912"
     CDS             complement(2389..2913)
                     /gene="grpE"
                     /locus_tag="VYO97_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1754660.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(2940..3974)
                     /gene="hrcA"
                     /locus_tag="VYO97_001913"
     CDS             complement(2940..3974)
                     /gene="hrcA"
                     /locus_tag="VYO97_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1754661.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4138..4647)
                     /locus_tag="VYO97_001914"
     CDS             complement(4138..4647)
                     /locus_tag="VYO97_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1754662.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         1350 a         1095 c          926 g         1566 t
ORIGIN      
        1 tgttctatag ttatcagctt gattacaaaa atcattataa acttttcgtg tattttttag
       61 atactcttca tccaatacac tcatcttact tttccgtaaa ctctccgtct acgacgtcat
      121 cgcctgcgtt tcctgttgct tgtgcgcctt ctgctccttc ttgagcttgt tgcgctgctg
      181 cggcttgttc gtagagttta acagcaagtc cttgagcttt ttcgttcaat gcttcaagtt
      241 ttgctttcat gtcgtccaag ttgttgtctt cttgagcttt cttaaggtca tcaagggcag
      301 cttgggcagc gtcacgttct gcgtcgaagc ctttaccttc agtttccttg attgtctttt
      361 cagtcgcaaa gattgcttgg tccacttcat tacgaaggtc tacttcttct ttacgtttct
      421 tatcggattc agcgtttgct tctgcatctt tcatcatgcg gtcgatttct tcgtcagtca
      481 aacctgagtt cgattggatg acaatagttt gttctttttg agttccaagg tctttggcct
      541 taacagacac gataccgttc ttgtcgatgt caaatgttac ttcgatttga ggaattccac
      601 gaggtgcagc tgggatatca gtcaattgga agcgtccaag agtcttgtta tctgctgcca
      661 ttgggcgttc accttgaaga acgtggatat caacggctgg ttggttgtct gctgctgttg
      721 agaagacttg tgatttagat gttgggattg tagtgttgcg atcgataagt tttgtaaata
      781 ctccacccat tgtttcgata ccaagtgaca atggcgttac atcaagaagg acaacatcct
      841 tgacatcacc agtaatcaca ccaccttgga tagccgcacc catagcaact acttcatcag
      901 ggtttactga tttgtttggt tctttaccag tttcagcttt aacagcttca acaacggcag
      961 ggatacgagt tgaaccacca acaaggataa cttcgtcgat ttctgacaag ctcaaacctg
     1021 catctgaaag ggcttgacga actggaactt ttgtacgttc aacaaggtca cgagtcaaat
     1081 catcaaattt cgcacgagtt aaagtcattt ccaagtgaag aggtccagcc tcacctgcag
     1141 tgataaatgg caagctgatt tgtgttgaag ttacaccaga aaggtctttc ttcgcttttt
     1201 cagccgcatc tttcaaacgt tgcattgcca tcttgtcagt agacaagtcg ataccgtttt
     1261 ctttcttgaa ttctgctacc aagtggtcaa tgattttttg gtcaaagtcg tcaccaccaa
     1321 gtttgttgtc ccctgcagtt gacaatacgt cgaagacacc gtcacccaat tcaaggatag
     1381 agacgtcgaa tgtaccacca ccaaggtcaa ataccaagat tttttcttct ttgtcagtct
     1441 tgtccaaacc ataagcaaga gctgctgcag ttggttcgtt aacaatacgt tctacttcaa
     1501 gaccagcaat tttaccagcg tcttttgttg cttgacgttg agcgtcgttg aagtaagccg
     1561 gaactgtaat aacagctttg gttactttct caccaaggta gtcttcagcg tagcctttca
     1621 agtattgaag gatcatagct gagatttctt gtggagtgta ttcttttcca tttgcagaaa
     1681 ctttttcaga agttcccatc ttagatttga tagagataac tgtatctggg tttgtaactg
     1741 cttgacgttt tgcagcatca ccaacgatga tttctccgtt tttgaatgag actacagatg
     1801 gagttgtgcg gtttccttct gggtttgcga tgattttgct ttcagttcct tcaagaactg
     1861 caactgctga gtttgttgta cctaagtcaa taccgataat tttagacatg tgtttttctc
     1921 cttgttagtt aattttctat ttttcttttt gatactcttc ttaagtggcg acgccgtcag
     1981 agcttgactt tgtcaatctc tttgactaaa ctttgagcct aaggtctcaa aatttgcgca
     2041 aatagcgcca cagcgaagag tatcgtcttt ggttcgcttc gctcactatt acaaagccaa
     2101 acatatttta tctttcttca tagtttattg tcgtttcgga caagttttct attatgtaaa
     2161 ttgcgacacg aagagtcgaa atcgatttta tttcgacgac gagttagtaa ggaagctaga
     2221 caaacgccat agcgactgcc gttttctgac gagttgcttt agctaccgtc agaattgccg
     2281 aatcgaacac gccctaagct ctgagtaaaa aagatagatt ttcttgtgtt catcgaacac
     2341 ttcgtcaatc tcctattttt actgcgagct ttttacgggc tttgtatctt agttatacac
     2401 cactaccatt gctgggcgta ggatgcggtc atggagtttg tagccttttt gaaagacttg
     2461 ggcgatggta tctactgggt gttcatcgtc tgctgggaga gtttggatgg ccatatggta
     2521 gttatggtca aattcgccat ctgctgcgat ttcttcaatt ccttcttctt tcaaagcgtg
     2581 aatcaagctt tcttgcacca tccccaagcc cttcttcaca tcatctgtca aaccttcaac
     2641 tgcaagtgca cgctcaaggt tgtcaagaga tggtaagatt gcttttgcca agtcctggct
     2701 acgataacgt tgcaagtttt gacgttcttc attggcacgg cgttggatat tttgcatttc
     2761 tgcatgagcg cgaagatatt tgttttcgaa ctcatctgca cgttcatttg ccaagtccaa
     2821 ctcagacttt tcaggagttg tttcttcagc tgtttccaca acttcctctt cttgaacttc
     2881 ttctacttct tcatttttta tatcttgggc catgttcgcc tcctttaatg atttcaatct
     2941 taatgtactt cataatgatt actgctgagg tagcggtaaa aatctgtcaa cttcatggtc
     3001 aaaacacggt tgaccacatt gacttgattg attagctgtt ggtaatccag attaactgga
     3061 ccgataatgg ctagaattcc aactccccga taaggaatga ggaacttact actaatcacc
     3121 gctaggtcag ctaaacagga ctcttgaccg tctgcaacac gaacattttg catctgatcc
     3181 tcacgcaacc cctcacgaat ctccaaggcc accttttgcg gttggtcaaa gaactgatag
     3241 gctgctagat tggcaaaatt caagagattg accttgcccg ccatcacaat gttttcgttg
     3301 aacatttcct taaagatgtg ttcaaagaga tcgatgacat tatccgttgt tgtaaagtaa
     3361 cgctggataa tctgcggaat ctccgtccga atcttgtagt gaatatctaa aacggtgtga
     3421 ccgaggaaac gttcctgaat gatgctcttc agtttcagca aatcctcctg caagaagttc
     3481 cttggaatca gaaactgact agtaaccgtt cgcgactcgt ctagggtaaa taccgccaag
     3541 gctgtatgtt gccccaaaac aacgatatca aaggctgtca aacgttgcct gctcggctca
     3601 acatccagtg ccactaccgt acagccactc aggtctgtta gcaagttagc agcctcttgc
     3661 agaatatcct ccaatttgaa gaattcctga tcaaaggctt tgacaatctc atatacctca
     3721 ttttcagcca gccggtcaaa atccagtgag tgtttcacat agtactgaaa accagcaaca
     3781 cttggcatcc gaccgcttga agtatgagcc ttctcaagca acccttgttt ttctagttcc
     3841 gccatgtcat tacgaatggt tgcactgcta gagttaatag actcttgcaa ggcttttgat
     3901 ccgacaggtt cgtgcgtttt ggtaaagatg tcaataatca gatttaaaat atcctgctga
     3961 cgctctgtaa ccatctcatc actcctctct gtctgatcaa ttagcactca atgcatccaa
     4021 gtgctaactt atgattctat tatacaccct tagaagagaa tgtcaagaca aaaactcaaa
     4081 aaattagcac tcttttatca agagtgctaa aaatcaatct gaagacctag aaccttgcta
     4141 ttcatctact cggtatttcc tttttagtct gaaataacca tgaattagat aagaaatcag
     4201 aaaagcatat aaaacaaaaa taagaaagaa agacatagaa agatcctctc taaacaaact
     4261 cgtacaaaca acaagaccac ctaagaaagt agttgtacag gaacgaagtc tagatcgcat
     4321 aacttcccat ttgagcccct tactcatata gacttgatcc tctggaagta aatcagaaga
     4381 cgatttacga agaatcgaac aagaacctgc tcctatcaat tcccaacctc tatctctaaa
     4441 atcttgcagt tcatgcttat attttttaag aaatctagaa tcatagatac ggtagatgac
     4501 atcgtctggt tgacattggt caaaatagaa caaaccaaaa cgactcgttc tatacctcca
     4561 acctttcaaa tgcatctcat gtaaatattc ttcttccttg tccaaatcaa caatagtgaa
     4621 aatccgaaat tctactctgc tattcattgt cttaccccaa aattagaaaa catgcctggc
     4681 gttatttatt agataattct ttccgctttt gactcaatct ccaaaaaata taagaaatct
     4741 gaatcgcaaa aactatcaat aaaacccaat ctattatgaa agtcaaaaac acttcccaac
     4801 tgaaagaact acctccagtg acaaactttg agaaaaacgg tagtagagct aaaaagagaa
     4861 gcaaaatagg aagcatccgc attgttaaaa tccttttggc ataaaaaaat ctttatttaa
     4921 acgaaaatat tatggca
//
LOCUS       DAVMAF010000101         4890 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMAF010000101 DAVMAF010000000
VERSION     DAVMAF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.102"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..132)
                     /locus_tag="VYO97_001915"
     CDS             complement(<1..132)
                     /locus_tag="VYO97_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComD"
                     /protein_id="HEV1754663.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     "
     gene            complement(153..278)
                     /gene="comC"
                     /locus_tag="VYO97_001916"
     CDS             complement(153..278)
                     /gene="comC"
                     /locus_tag="VYO97_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1754664.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(443..516)
                     /locus_tag="VYO97_001917"
     tRNA            complement(443..516)
                     /locus_tag="VYO97_001917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Arg,seq:tct)
     gene            complement(560..1039)
                     /gene="rlmH"
                     /locus_tag="VYO97_001918"
     CDS             complement(560..1039)
                     /gene="rlmH"
                     /locus_tag="VYO97_001918"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1754665.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            1222..2403
                     /locus_tag="VYO97_001919"
     CDS             1222..2403
                     /locus_tag="VYO97_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1754666.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2461..3219
                     /locus_tag="VYO97_001920"
     CDS             2461..3219
                     /locus_tag="VYO97_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1754667.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            3432..4793
                     /gene="dnaA"
                     /locus_tag="VYO97_001921"
     CDS             3432..4793
                     /gene="dnaA"
                     /locus_tag="VYO97_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1754668.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1711 a          868 c          808 g         1503 t
ORIGIN      
        1 ttctgttagt aatgtataag caccaaaaac gaataattct tttctattta tttgaccttt
       61 acaaataaaa tggtaactgt gactaataat taaaaaatga acaataaccg tcccaaatcc
      121 aaataaatcc attactcttt ccccttattt cattactttt ttctttgtaa aataaaatca
      181 cggaagaatt ttgacaacct catctcccca cctttaatct tttgtaagtc tttttccttc
      241 aaagctacaa actgttccaa tttaactgtg tttttcataa taaaatctcc taaaatgttt
      301 tttcttgtaa gctaacttac aaaaaccatt atacaaaatg gaatttcgtt ttagataaaa
      361 ttctctcaac tgtcattttt ttctcccaaa gtgtactttt ttaagaaaaa agccgggaaa
      421 attcccagct ttgctattat attgatccca gcaggattcg aacctgcgac cgttcgctta
      481 gaaggcgaat gctctatcca gctgagctat gagacctaat acaattattc taccaaaaat
      541 tcaattaaaa gtcaattttc tatttatggt aggggaatcc ctgctgaatc gtaaaagcgc
      601 gatagatttg ttcaacaaga actagtctca ttaactgatg gggtaaggtt aggcgaccaa
      661 aactgacaga aagattggct ctatttttta cagatgatga caatcctaaa cttcccccaa
      721 taataaaagt aagagtagaa aatcctttta tagaagtttc ttctaactgc ttactaaatt
      781 cttctgagaa gaaagttttc ccttcaatgg ctaacacaat aacgaaatca cggtcagcaa
      841 tttttgataa aattctctga ccttctattt ctaaaatctt ttgattttct gattcactgg
      901 ccttatctgg tgttttttca tctgataact caatcatttc aaacttagca aatctagaaa
      961 ttcgttttga atactctgcg ataccatctt ttaaatactt ttctttcagt ttcccaactg
     1021 ttacaacttt aattttcatg actctattct aacatattct ctattttttc acatcttatt
     1081 cacaaaataa aaaatagatt tcaattaaga aaatcacagt ttcaaaagag ttatccacag
     1141 tttgtgtaaa acttttgtgt ttaagttata attaagctag tcagtttata ctttcagtaa
     1201 ttcaaacata tggaggcaaa tatgaaacat ctaaaaacat tttacaaaaa atggtttcaa
     1261 ttattagtcg ttatcgtcat tagctttttt agtggagcct tgggtagttt ttcaataact
     1321 caactaactc aaaaaagtag tgtaaacaac tctaacaaca atagtactat tacacaaact
     1381 gcctataaga acgaaaattc aacaacacag gctgttaaca aagtaaaaga tgctgttgtt
     1441 tctgttatta cttattcggc aaacagacaa aatagcgtat ttggcaatga tgatactgac
     1501 acagattctc agcgaatctc tagtgaagga tctggagtta tttataaaaa gaatgataaa
     1561 gaagcttaca tcgtcaccaa caatcacgtt ataaatggcg ccagcaaagt agatattcga
     1621 ttgtcagatg ggactaaagt acctggagaa attgtcggag ctgacacttt ctctgatatt
     1681 gctgtcgtca aaatctcttc agaaaaagtg acaacagtag ctgagtttgg tgattctagt
     1741 aagttaactg taggagaaac tgctattgcc atcggtagcc cgttaggttc tgaatatgca
     1801 aatactgtca ctcaaggtat cgtatccagt ctcaatagaa atgtatcctt aagatcggaa
     1861 gatggacaag ctatttctac aaaagccatc caaactgata ctgctattaa cccaggtaac
     1921 tctggcggcc cactgatcaa tattcaaggg caggttatcg gaattacctc aagtaaaatt
     1981 gctacaaatg gaggaacatc tgtagaaggt cttggtttcg caattcctgc aaatgatgct
     2041 atcaatatta ttgaacagtt agaaaaaaac ggaaaagtga cgcgtccagc tttgggaatc
     2101 cagatggtta atttatctaa tgtgagtaca agcgacatca gaagactcaa tattccaagt
     2161 aatgttacat ctggtgtagt tgttcgttcg gtacaaagta atatgcctgc caatggtcac
     2221 cttgaaaaat acgatgtaat tacaaaagta gatgacaaag agattgcttc atcaacagac
     2281 ttacaaagtg ctctttacaa ccattctatc ggagacacca ttaagataac ctactatcgt
     2341 aacgggaaag aagaaactac ctctatcaaa cttaacaaga gttcaggtga tttagaatct
     2401 tgattgacat ctatgtaaag aaagctttac ataagagaaa agatgtgtta gtgtagaatc
     2461 atggaaaaat ttgaaatgat ttctatcaca gatatacaaa aaaatcccta tcaaccccga
     2521 aaagaatttg atagagaaaa actagatgaa ctagcacagt ctatcaaaga aaatggggtc
     2581 attcaaccga ttattgttcg tcaatctcct gttattggtt atgaaatcct tgcaggagag
     2641 agacgctatc gggcttcact tttagctggt ctacggtcta tcccagctgt tgttaaacag
     2701 atttcagacc aagagatgat ggtccagtcc attattgaaa atttacagag agaaaattta
     2761 aacccaatag aagaagcacg cgcctatgaa tctctcgtag agaaaggatt cacccatgct
     2821 gaaattgcag ataagatggg caagtctcgt ccatatatca gcaactccat tcgtttactt
     2881 tccttgccag aacagattct ttcagaagta gaaaatggca aactatcaca agcccatgcg
     2941 cgttccctag ttgggttaaa taaggaacaa caagactatt tctttcaacg gattatagaa
     3001 gaagatattt ctgtaaggaa attagaagct cttctgacag agaaaaaaca aaagaaacag
     3061 caaaaaacta atcatttcat acaaaatgaa gaaaaacagt taagaaaact actcggatta
     3121 gatgtagaaa ttaaactatc taaaaaagac agtggaaaaa tcattatttc tttttcaaat
     3181 caagaagaat atagtagaat tatcaacagc ctgaaataag gctgttcttt tattttttta
     3241 tctcacaagg ttatccacta tgtttttcga taaaaagctt aataaatcaa taatttcttc
     3301 ttttatcccc aacctgtgga taaagtttgg taacattgtg gattattttt cacagcttgt
     3361 ggaaaattct tgctatctat ggtaaaatat ctctagtatt aaacttttaa atagtaaagg
     3421 aggagaaagg attgaaagaa aaacaatttt ggaatcgtat attagaattt gcacaagaaa
     3481 gactgactcg atccatgtat gatttctatg ctattcaagc tgaactcatc aaggtagagg
     3541 aaaatgttgc cactatattt ctacctcgct ctgaaatgga aatggtctgg gaaaaacaac
     3601 taaaagatat tattgtagta gctggttttg aaatttatga cgctgaaata actccccact
     3661 atattttcac caaacctcaa gatacgacta gctcacaagt tgaagaagct acaaatttaa
     3721 ctctttataa ctatagtcca aagttagtat ctattcctta ttcagatacg ggattaaaag
     3781 aaaagtatac ctttgataac tttattcaag gggatggaaa tgtttgggct gtatcagccg
     3841 ctttagctgt ctctgaagat ttggctctga cctataaccc tctttttatc tatggaggac
     3901 caggccttgg taagactcac ttattaaacg ctattggaaa tgaaattcta aaaaatattc
     3961 ctaatgcgcg tgttaaatat atccctgccg aaagctttat taatgacttt cttgatcacc
     4021 taagacttgg ggaaatggaa aagtttaaaa agacctatcg tagtcttgat cttttgttaa
     4081 tcgatgatat ccagtcactc agcggaaaaa aagttgcaac tcaggaagaa tttttcaata
     4141 cctttaacgc ccttcatgac aagcaaaaac agattgtcct aacgagtgat cgtagtccaa
     4201 aacatctaga agggctcgag gagaggcttg tcacgcgttt tagttgggga ttgacacaaa
     4261 ctatcacacc ccctgacttt gaaacacgta ttgccatttt acaaagtaaa acggaacatt
     4321 taggctacaa tttccaaagt gatactctag aatacctagc tgggcaattt gattcaaatg
     4381 ttcgagatct tgagggagcc atcaacgaca tcactttaat tgccagagta aaaaaaatca
     4441 aggatatcac tattgatatt gctgcagaag ccattagagc ccgcaaacaa gatgttagcc
     4501 aaatgctcgt catcccaatt gataaaatcc aaactgaagt tggtaacttt tatggtgtta
     4561 gtatcaaaga aatgaaggga agtagacgcc ttcaaaatat tgttttggcc cgtcaagtag
     4621 ccatgtattt atctagagaa ctaacagata atagtcttcc aaaaattggg aaggaatttg
     4681 ggggaaaaga tcataccaca gtcattcatg cccatgccaa aataaaatct ttgattgatc
     4741 aagacgataa tttacgttta gaaattgaat caatcaaaaa gaaaatcaaa taatttgtgg
     4801 ataactttta gttttttatc ttttttatcc acatttttta aacaagctaa aaaacttgat
     4861 atgacttgtt taaaggctgt tttccacaga
//
LOCUS       DAVMAF010000102         4887 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMAF010000102 DAVMAF010000000
VERSION     DAVMAF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.103"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            138..1007
                     /gene="rfbA"
                     /locus_tag="VYO97_001922"
     CDS             138..1007
                     /gene="rfbA"
                     /locus_tag="VYO97_001922"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1754669.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAKKEKGATVFGYHVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            1008..1601
                     /locus_tag="VYO97_001923"
     CDS             1008..1601
                     /locus_tag="VYO97_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1754670.1"
                     /translation="MTDNFFGKTLAARKIEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            1614..2663
                     /gene="rfbB"
                     /locus_tag="VYO97_001924"
     CDS             1614..2663
                     /gene="rfbB"
                     /locus_tag="VYO97_001924"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1754671.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            2729..3580
                     /gene="rfbD"
                     /locus_tag="VYO97_001925"
     CDS             2729..3580
                     /gene="rfbD"
                     /locus_tag="VYO97_001925"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1754672.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3643..3977
                     /locus_tag="VYO97_001926"
                     /pseudo
     CDS             3643..3977
                     /locus_tag="VYO97_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            4148..4264
                     /locus_tag="VYO97_001927"
     CDS             4148..4264
                     /locus_tag="VYO97_001927"
                     /inference="COORDINATES: protein motif:HMM:NF015247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754673.1"
                     /translation="MPVRLTYDNNYFNDTYQGIPIGGYTQIVEKCWIMKILM"
     gene            4240..4825
                     /locus_tag="VYO97_001928"
                     /pseudo
     CDS             4240..4825
                     /locus_tag="VYO97_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         1563 a          831 c         1096 g         1397 t
ORIGIN      
        1 acctagccta tcagttaaat tagaatatca actttgatta attaaaaatt agcaaaattt
       61 attgacattt tgtttttata aattgcaata aaggtctaat tctgaatttc agtgaatata
      121 agaaagggat cctactaatg aaaggtatta ttcttgcagg tggttcgggg acacgattat
      181 atcctttgac tcgggctgca tcaaaacaac ttatgccggt ttatgataaa cccatgattt
      241 actatccact ttcaacattg atgttggctg ggattaggga tattttgata atctcaactc
      301 ctcaagattt gcctcgtttt aaagagcttc ttcaagacgg atctgagttt gggattaaac
      361 tttcttatgc agagcaacca agtccagatg gtttggcaca agcctttatc attggggaag
      421 agtttattgg tgatgatagt gttgctttga tcttggggga caatatctat cacggccctg
      481 gtttgagcaa aatgcttcaa aaagcagcca agaaagagaa aggtgcgact gtctttggct
      541 accatgtcaa ggatccagag cgctttggtg tggttgagtt tgatacagac atgaatgcta
      601 tttccataga agaaaaaccg gagtatcctc gctccaacta tgcagttaca ggtctctatt
      661 tctatgataa tgatgtagta gagattgcca aaagtattaa accaagtcct cgtggtgaac
      721 tggaaattac agatgtaaac aaggcttatc tagaacgtgg tgatttgtct gttgagctta
      781 tgggacgtgg ctttgcttgg ttggatactg gcactcatga aagtttacta gaggcttcgc
      841 agtacatcga aacagtccaa cggatgcaaa atgttcaggt agcaaactta gaagaaattg
      901 cctatcgtat gggttatatc agtcgagaag atgtattggc cttagcccaa ccacttaaga
      961 aaaatgaata cggacagtat ctgctccgtt tgattggaga agcatagatg acagataatt
     1021 ttttcggtaa gacgcttgcg gcacgcaaga ttgaagctat tccaggcatg ttggagtttg
     1081 atatccccgt tcatggagat aatcgtggct ggtttaaaga aaatttccaa aaggaaaaaa
     1141 tgcttccgct tggatttcca gagtctttct ttgcagaagg aaaattgcaa aacaatgtat
     1201 ccttctcacg taaaaatgtc cttcgagggc tccacgcaga gccttgggat aagtacatct
     1261 ctgtagcaga tggagggaaa gttctgggtt cttgggttga tctacgcgag ggtgaaacct
     1321 ttgggaatac ctatcagaca gtaattgatg caagcaaggg aatctttgtt cctcgaggcg
     1381 tagctaatgg cttccaagtt ctatcagata cagtgtcata tagctatctg gtcaatgatt
     1441 actgggctct cgagctcaaa cccaagtatg cctttgtgaa ctacgctgat ccaagccttg
     1501 gtattgaatg ggaaaatatt gcagaagcag aggtttcaga agcagataaa catcatcccc
     1561 tacttaagga tgtaaaacct ttgaaaaaag aagatttgta aaaaggaaag aatatgactg
     1621 aatacaaaaa tattatcgtg acaggtggag ctggctttat cggttctaac tttgtccatt
     1681 atgtttacga gaactttcca ggtgttcacg tgacagtcct agataagttg acttatgctg
     1741 gaaatcgcgc gaatattgag gaaattttag gtaatcgtgt tgagttagtt gttggtgaca
     1801 ttgctgatgc ggagttggta gacaagttgg ctgctcaagc agatgctatc gttcattatg
     1861 cagcggaaag ccacaatgat aattcgctca atgatccatc gccatttatt catactaact
     1921 tcattggaac ctatactctt ttagaagctg ctcgtaagta tgatattcgc ttccaccatg
     1981 tatcgacaga tgaagtttat ggggatctcc ctttacgcga agatttgcca ggtcatggag
     2041 aagggccggg tgagaaattt acggctgaaa ccaagtacaa tccaagctcg ccttactcat
     2101 caaccaaggc agcctcagat ttgattgtca aagcctgggt gcgttctttt ggagtcaagg
     2161 caacgatttc caactgttca aataactacg gtccttatca acatatcgaa aaattcatcc
     2221 cacgtcagat tactaacatc ctaagtggta tcaagccaaa actttacggt gaaggtaaaa
     2281 acgttcgtga ctggattcat accaatgacc attcttcagg agtttggaca atcttgacaa
     2341 aagggcaaat cggtgaaacc tacttgattg gggctgatgg tgagaagaac aataaggaag
     2401 ttttggaact tatccttaag gaaatgggac aagctgcgga tgcctatgat catgtgactg
     2461 accgtgcagg acatgacctt cgctatgcga ttgatgctag caagctccgt gatgagttgg
     2521 ggtggaaacc tgaatttacc aactttgaag ctgggctcaa ggcaacaatc aagtggtata
     2581 cagataacca agaatggtgg aaagcagaaa aagaagctgt tgaagccaat tatgctaaga
     2641 ctcaggagat tattacagta taaaaagcag gaaatagctg ctttttattg ctatattggg
     2701 aagagttata tattagaaag gtctagagat gattttaatt acaggggcaa atggccaatt
     2761 aggaacggaa cttcgctatt tattggatga acgtaatgaa gaatacgtgg cagtagatgt
     2821 ggctaagatg gacattacca atgaagaaat ggttgagaaa gtttttgaag aggtgaaacc
     2881 gactttagtc taccattgtg cagcctacac cgctgttgat gcagcagagg atgaaggaaa
     2941 agagttggac ttcgccatca atgtgacggg gacaaaaaat gtcgcaaaag catctgaaaa
     3001 gcatggtgca actctagttt atatttctac ggactatgtc tttgacggta agaaaccagt
     3061 tggacaagag tgggaagttg atgaccgacc agatccacag acagaatatg gacgcactaa
     3121 gcgtatgggg gaagagttag ttgagaagca tgtgtctaat ttctatatta tccgtactgc
     3181 ctgggtattt ggaaattatg gcaaaaactt cgtttttacc atgcaaaatc ttgcgaaaac
     3241 tcataagact ttaacagttg taaatgatca gtacggtcgt ccgacttgga ctcgtacctt
     3301 ggctgagttc atgacctacc tagctgaaaa tcgtaaggaa tttggttatt atcatttgtc
     3361 aaatgatgcg acagaagata caacatggta tgattttgca gttgaaattt tgaaagatac
     3421 agatgtcgaa gtcaagccag tagattccag tcaatttcca gccaaagcta aacgtccgct
     3481 aaattcaacg atgagcctgg ccaaagccaa agctactgga tttgttattc caacttggca
     3541 agatgcattg caagaatttt acaaacaaga agtgagataa gtagtagaat gattttctag
     3601 tctaataaaa gaggcagata atgaactcca aaggagctta agatatacaa ttatcttgtt
     3661 gttggtgctg gtctctttgg tgcagtcttt gcccatgaag cagccttaaa aggaaaaaag
     3721 taaaagttat tgaaaaacga aatcatatcg cgggtaatat ctatactcgt gaagaggaag
     3781 gaattcaagt tcatcaatat ggtgctcata tcttccatac ttctgataag gagatctggg
     3841 agtatgtaaa tcagtttgca gagtttaacc gttataccaa ttctcctgtt gcaaactata
     3901 agggtgagat ttataacctt ccttttaata tgaatacttt caataaactc tggggagttg
     3961 taacgccagc agaagcacaa gctaagattg aggaacaacg tgctatttta aatggtaaaa
     4021 ctcctgaaaa tttggaagaa caggcgattt ctcttgtagg tacagacatc tacgaaaaat
     4081 taatcaaaga ctatacagag aaacagtggg gcaaatcaac tactgaactt ccatccttta
     4141 ttattgtttg ccagtacgcc tgacctatga taacaattat tttaacgata cctatcaagg
     4201 gattccaatt ggtggataca ctcaaatagt tgaaaaatgt tggatcatga aaatattgat
     4261 gtagaaacaa acgttgattt ctttgtgaat aaagagcaat atctgaaaga ttttcctaag
     4321 attgtcttta ctggtatgat tgatgaattc tttgactata agttgggcga actagagtac
     4381 tgtagtcttc gttttgaaaa tgagaccttg gatatggaaa attaccaagg aaatgcagtt
     4441 gtgaactata cggatgcaga aaccccatat actcgcatta ttgaacacaa acattttgag
     4501 tttgggagtc aagcaaagac tatcattact aatagaacat tctaaaacat gggaaaaagg
     4561 tgatgagcct tattatccag ttaataatga tcgtaataat catttgtata aatcatataa
     4621 aaaacttgct gatgagcaag ggaatgttat ctttggtggc cgcttaggac actatcgtta
     4681 ttacgatatg caccaagtaa ttggagcagc tttgcagtgc gtgagaaatg agttagattc
     4741 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagcactg
     4801 ttttgaggtt gcagatggaa gctgacgcgg tttgaagaga ttttcgaaga gtataaacaa
     4861 gtaaaactga ctaccagtta ttattta
//
LOCUS       DAVMAF010000103         4876 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMAF010000103 DAVMAF010000000
VERSION     DAVMAF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.104"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..243
                     /locus_tag="VYO97_001929"
     CDS             <1..243
                     /locus_tag="VYO97_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754674.1"
                     /translation="SGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLP
                     VFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
     gene            629..838
                     /locus_tag="VYO97_001930"
     CDS             629..838
                     /locus_tag="VYO97_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1754675.1"
                     /translation="MAKNLKLKLARVELDLTQGQLADAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            828..1316
                     /locus_tag="VYO97_001931"
     CDS             828..1316
                     /locus_tag="VYO97_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754676.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1432..2892
                     /locus_tag="VYO97_001932"
     CDS             1432..2892
                     /locus_tag="VYO97_001932"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1754677.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            2889..3713
                     /gene="nadE"
                     /locus_tag="VYO97_001933"
     CDS             2889..3713
                     /gene="nadE"
                     /locus_tag="VYO97_001933"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1754678.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAIEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4063..4614
                     /locus_tag="VYO97_001934"
     CDS             4063..4614
                     /locus_tag="VYO97_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754679.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1457 a          897 c         1090 g         1432 t
ORIGIN      
        1 agtggcttgg tatttggtat tttagtagct ttactactct ttatccctaa tatattaaat
       61 acgatgatga tgaatactag taatgatttg tttatcaaag caattgaact tcttcctttt
      121 tattctttac ctgtatttgt ggcttcaaat acgatgtcta ttagtcagta tcttgtgcta
      181 atcactacaa tcattttatt gtactttttc actctcaaga aaagcaagaa gtattcattt
      241 tagtttcatt tagtattatt ttgcatacct aaaatacaat aaaaaaatca gtcatcttgg
      301 tatgctcctg ctttcactat tcaacacgtt tttgacttat actaggctca tttccaaaag
      361 cattatataa tagtgatatg aaaccaacta aactaaacaa gaaatataag caataaaaac
      421 tcgtttaaaa gattctacta aagctaatac taaataaaaa taaaagagta aactaggaag
      481 tttatttcaa acaacctaaa atactgattt tcggctgaag ataatactgg agtgcaaatt
      541 aatggggtta taataaatag ctgatagctt gtgttggttt tggatttttt aagagtagat
      601 gagtattaaa actataagga ggacgaaggt ggctaaaaat ttaaaattaa aattagctcg
      661 ggtagagctt gatttaactc aaggtcaact agcagatgct gtcggggtga cgcgccagac
      721 tattggtttg atagaggcgg ggaaatacaa tcccagtctc tcgctctgcc agtccatttg
      781 cagatgttta gggaaaaccc tagaccaact attttgggag gaagaagatg aaaaatagat
      841 tttattatta tcaattacta gacgaaagag aagaacaact gttcaataaa gcgggctctg
      901 aaagtttcta tatctgcatc gctttgtcgc tcctatctta tatcatttca gtattagcac
      961 caagcctttt taattctaat atgctgctaa tcgttatcat cataggaaca ttttactttt
     1021 tcaatcgtgc ccgttatctg ggagtgacct actatagtcg ttttcatttt acgattttgg
     1081 gttgtttttt cctaaccttg gctattacgg ctcttttgat gttgcagaat tatcaattca
     1141 acatagaaat ttatcagcac aatcctttga attttaaata cctgtctgct tgggtcatta
     1201 cttatgtcat ttaccttccg tgggtcttta ttggcaatct tggtcttaag agctatggcg
     1261 aatgggctca gaaaaaattt gaacaagata tggatgaatt ggagagtgga gaatagcttg
     1321 ttactctttt ctcaatccag ctaaaatgtg atataatagt actaatttat tggaatacat
     1381 gaaagttctt gaaaattttc atgggtttct agctaaggaa gtaggaaaag tatgtatcca
     1441 gatgatagtt tgacattgca cacggacttg taccagatca acatgatgca ggtttacttt
     1501 gaccaaggga ttcacaataa gaaggcggtc tttgaggtgt atttccgcca acagcctttt
     1561 aagaacggct atgcggtttt tgcaggtttg gaaagaattg tgaactatct tgaagacttg
     1621 cgtttttcag atagtgatat agcctatttg gagtcgcttg gttatcatgg ggcgttcttg
     1681 gattaccttc gcaatttcaa gttggagttg accgttcgtt ctgcccaaga aggggatttg
     1741 gtttttgcta atgaaccgat tgtgcaggtg gaaggacctc tagcccaatg tcagttggtc
     1801 gaaacggctc ttttgaacat cgtcaactac cagaccttgg tggcgacgaa ggcagctcgt
     1861 attcgttcgg ttatcgaaga tgaacccttg atggagtttg ggacacgtcg ggctcaagaa
     1921 atggatgcgg ccatctgggg aacacgtgca gcggtgattg gtggcgccaa tggaaccagc
     1981 aacgtgcgtg cgggtaaact ctttgacatt cctgttttgg gaacccatgc ccatgccttg
     2041 gtgcaggttt atggcaatga ctatgaggct ttcaaggctt acgctgcgac ccacaaaaat
     2101 tgtgtctttc ttgtggatac ctatgatacc cttcgcatcg gtgtaccagc tgccattcag
     2161 gtggcgcgtg aactgggtga tcagattaac tttatgggtg tgcggattga ctctggggac
     2221 attgcttaca tttctaagaa agtccgtcag caactggacg aggctggatt tacagaggct
     2281 aagatttatg cttctaatga tctagatgaa aataccatcc ttaacctcaa gatgcaaaag
     2341 gccaagattg atgtctgggg tgtgggtacc aagctgatta cagcctatga ccagccggct
     2401 cttggggcgg tttacaagat tgttgcaatt gaagatgaaa ctggtcagat gcgcaatacg
     2461 attaagctgt ctaataatgc ggaaaaagtg tcgacgccag gtaagaagca ggtgtggcgc
     2521 attaccagtc gtgaaaaagg caagtcagaa ggtgactaca tcacttatga tggtgtggat
     2581 attagcgaca tgacagaaat caagatgttc catccgacct atacatacat caagaagacg
     2641 gttcgtaatt ttgatgccgt tcctctcttg gtggatatct tcaaagaagg aatattagtt
     2701 tacaacttgc ctagtttgac tgacattcag gattatgccc gtaaagaatt tgacaagttg
     2761 tgggatgagt ataagcgtgt gctcaatccg cagcactatc cagtggattt ggcacgtgat
     2821 gtatggcaag ataagatgga cttgattgat aagatgcgca aggaagccct tggtgaagga
     2881 gaagaagaat gagtttgcaa gaaacgatta tccaagagct gggtgtcaaa ccagtgattg
     2941 atgcccagga agaaatccgt cgttctattg atttcttaaa aagatatctg aaaaaacatc
     3001 ccttcctaaa aacctttgta ctagggattt ctgggggaca agactcaacc ttggcaggac
     3061 gtttggcgca attagctata gaagaactgc gagctgaaac gggagacgac agctacaaat
     3121 ttatcgctgt ccgcctgcca tacggagtgc aagctgatga agcagatgct caaaaagccc
     3181 tagccttcat ccagccagat gtcagcttgg ttgtgaatat caaggaatca gctgatgcca
     3241 tgacagctgc agttgaagcg acaggtagtc ctgtttcaga cttcaacaag gggaatatca
     3301 aggcacgttg ccgtatgatt gctcagtatg cccttgctgg ttcccatagc ggagcggtca
     3361 ttggaacaga ccacgccgca gaaaatatca caggtttctt taccaagttt ggtgacggcg
     3421 gtgcggatat tctccctctt taccgcctca ataaacgcca aggaaaacag ctcttgcaga
     3481 aacttggcgc agagccagcc ctttatgaaa aaatcccaac ggcagaccta gaagaagata
     3541 aaccaggcct agctgacgaa gtcgcacttg gagtcaccta cgcagagatt gacgactacc
     3601 tagaaggcaa aacaatcagc ccagaagctc aagcgaccat tgaaaactgg tggcacaaag
     3661 gccaacacaa acgccactta cccatcaccg tatttgatga cttttgggag taaaaaggtc
     3721 cgggggacct ttttagcttc ttgccctgaa attaaaaagc aagaaaaacc tccactggag
     3781 gttttcagcc tctcatcttg aaataagaaa gtgagagaag gtccggggga tcttgaacct
     3841 cgagtttaga aataagaaaa tgaggcagat tcggtaactc gaagagttcg atttcatcgt
     3901 cttacccctg caacgatgac taggtttgaa aaagcttgct agatcgcatt tcaaaccagg
     3961 cagcgactgc gtcaagaaat tagaagacaa actcgttttc tagctgttac tgagttgagc
     4021 ctttttacta cgagcataga aataaggaag tgaggtagca tcatgaaatc tattggtacg
     4081 caaatattac agacagaacg tttgatttta agaagatttg tggagagtga tgcagaagcc
     4141 atgtttcaaa attgggcttc atccgctgag aatctgacct atgttacctg ggatccccat
     4201 cctgatgtcg aaatcactcg aaactcgatt cgcaattggg ttgcttccta tactaatctc
     4261 aactattaca aatgggccat ttgtctaaaa gaaaacccag agcaagtgat aggagatatc
     4321 agcatcgtta agatagacga ggctgattta agctgtgaaa tcggctatgt gctaggcaag
     4381 gcttactggg gacatggtat gatgacagag gccttgaaag ctgtcttgga cttttgtttt
     4441 actcaagcag gttttcaaaa ggtcagagca cgttatgcca gtctcaaccc agcttcaggt
     4501 cgtgtcatgg aaaaggctgg aatgtcctat ctacaaacca ttgttaatgg tgtagagaga
     4561 aaaggctatc ttgcggatct tatttattat ggtataagta gggaagaatg ttgaattcta
     4621 ttttctgttt ctatcgaagt caactattta ttgtaaatat aataattagc attccaagtt
     4681 tatttgcaat tttaaaatag catattgatt agtacaagac agatgttcta gttccttctt
     4741 taatctggtt tagtattagt taaaaaatcg ctttaagctt gtaactaaga gggagctaat
     4801 cgactagatt ctccagccga acaggtggta atgtactttt tatagtgtaa tcctagctgt
     4861 tgttaaattt aaaata
//
LOCUS       DAVMAF010000104         4829 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMAF010000104 DAVMAF010000000
VERSION     DAVMAF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.105"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(111..914)
                     /locus_tag="VYO97_001935"
     CDS             complement(111..914)
                     /locus_tag="VYO97_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1754680.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1195..1950)
                     /locus_tag="VYO97_001936"
     CDS             complement(1195..1950)
                     /locus_tag="VYO97_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1754681.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2020..4671)
                     /locus_tag="VYO97_001937"
     CDS             complement(2020..4671)
                     /locus_tag="VYO97_001937"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1754682.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKNLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4683..>4829)
                     /locus_tag="VYO97_001938"
     CDS             complement(4683..>4829)
                     /locus_tag="VYO97_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1754683.1"
                     /translation="YISKISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIV
                     NKSN"
BASE COUNT         1398 a         1042 c          931 g         1458 t
ORIGIN      
        1 ttgtctaact ttttggggtc agtacaccta aaactttgat gatatacgtt ttcttgtgag
       61 aatatttact tcatttttgc ctaaaattca atgtttactc agtatttgga ttatgaaaaa
      121 tcgaggtcta aatctagata cattttttct gaagacaaat cattttgacc accgagcaag
      181 agattttcaa aaaaagctgt taaaaactca gaacgtcgct gtaaaatctt tgcattatct
      241 aataccaagg catcacgaaa atatttggaa tgttgctgaa atggtgtatt atcaatatca
      301 aaaccaaact cacgaagata ctgaatcaaa aagaccgtta ctgtccgagt gtttccttcg
      361 cgaaatggat gaatctgcca gattcctgaa ataaaatgct ggatttgttt aaccacatcc
      421 gcctgagtta gtgtcgcata tgcaacttgt ttttcctgat taaaatcata atctaaggtc
      481 atttgaatca tggagtaatc agagtacaca acactttcac cattcaaaac aggttcattc
      541 tttgtgatat tggtctgacg aaattgcccc accggaatag agggttcaaa tatatcttga
      601 aacaactcct tatgaatagc aagtaaggtc gcaggactaa agctaaagcc tcttcgagac
      661 aatagttcta caatacgtag agaaaccaag tctgcctctt ccgtacttgc atcaatagta
      721 tggtgataag ccgttgcatc ctcataaacc tgctcataag tcagttctcc ccgggactgt
      781 ttctcagcca aagattccat atacgctgat ggcactagat tgtcaacttt ctgcagacca
      841 aaacctatcc gccataaatc acgcttcgct tcataagaca agtttggatt gtcaatgttg
      901 taagttggtt gcatagaaat atcctttcaa ttgtttttct tcattaccag taaatcacga
      961 aattggttgg aaactggctt aaactataaa aagtcctcac aataggttct atgatatata
     1021 tatatatata cttcatgtaa ataataaaag agttataatc agcactactg ataaattcaa
     1081 taccgatttc gccatcttaa aacttagtca aacgaatacc gttgatttcc agttcattaa
     1141 gtcgttgacc tgtcaagact tctcgttcct tcaactggaa aagttaaggg ggaactaata
     1201 caaatcctct acgcttctat attcaatgat ttcatttttg ataatattct ttttaaactc
     1261 aaaatgtttt aaaggattat cgattaacac taactttgga atatcatcta gatttgtgac
     1321 caaggataaa ataaaatccg attgaaactc cttgactttt tcaaattctt ttgcagttaa
     1381 acgcacacgc cccttagatt ttcggatacc ttttacttcg gcaaggtaag aatactcttg
     1441 gacatctacc tgaaagtcat atccatcacc ataaagacgg gcatcggtta actgtccacc
     1501 ttggaatttt tcttctttat caaaatgaag gataaaataa ttctcagcct ccattcccgt
     1561 ttcctgtaga cgtttgaatt tagttcgaac tattggtttt tcaacgatgg ttatgcctgt
     1621 tttcttccct tcactggcaa gtaacatttt aactatttca gcataactat gaacatcttc
     1681 atttccaaac atcatatcaa ttaaatcttt acgaaaacga taaacttcag ctttctgcca
     1741 ccaacctttt cgattattgt caaaataagg atcaaataaa tccattctat tttttacaac
     1801 tccagttgtt tcagcaattc ccagagaaac cacatagcga taaaactccg acttgctaga
     1861 gcattgaaat tcttttataa aaagcttgtc aaacttggca agaccgtagc caattaaatt
     1921 taacaattca taatgagggt gtttggacat cactttctcc atcaatagat attgatttca
     1981 ttataccaaa aaacacggct catcaccgtg tttctgtgtt tatttcacca acttcttcat
     2041 ctcatcaata cgtgctacgg tcgcatcata cttggcttgg tagtcagctt gtttgtcgcg
     2101 ttctttttgg acgacttctg gtttggcgtt ggctacgaag cgttcgttag agagtttctt
     2161 accgaccata tccagttctt tttgccattt agcgagttcc ttgtcgagac gagccagttc
     2221 ttcttcgaca ttgagaaggt ctgccagtgg caagtagatt tctgctcctg tgatgacgct
     2281 tgacatagcc agttcaggtg cagggatggt tgatgcgatt tccaaatgtt ctggatttgt
     2341 aaagcgcttg atgtagttga cattgctgtt aaagaaggct tccaagtcac tatcgctagt
     2401 cttaacaagg attgtgatag gtttgcttgg tgctacattt acttccgcac gcgcattccg
     2461 aacagcacga atcaagtttt tgagactttc cacaccagtg tgggcagcaa ggtcttcaaa
     2521 ggctgggttg acagttgggt atgctgctgt cacgatagag ccttcagcgt actgaccaaa
     2581 gatttcttct gtcacgaacg gcatgattgg gtgaaggaga cgaaggattt tatccagcgt
     2641 atagaggaga acagatcgag taattacctt atcgtcttca ttgtcgctgt aaagaacttc
     2701 cttggtcaac tcaacatacc agttggcaaa ttcttcccaa atgaagttgt aaaggatatg
     2761 accagccaca ccaaactcaa acttatcaaa gttttcagta acttttgcaa tggtttcgtt
     2821 gagattgtgg agaatccagc ggtccgtcac attaccagcc tcacctgttg caacttttgt
     2881 gacattgtca tgcgccacat ccagcgtcaa acctccattg ttcatgagga tatagcgaga
     2941 aatgttccaa atcttgttaa taaagttcca tgaagcatcc attttctcgt aagagaaacg
     3001 aacgtcttga ccaggagctg agccgtttga caagaaccaa cgaagactat ctgtaccata
     3061 tttatcaata acatccattg ggtcaatgcc atttccaaga gatttagaca tcttacgtcc
     3121 ttcttcatca cgaattagac catggataag gacgttttgg aatggttgac ggcctgtgaa
     3181 ttccaatgac tggaagatca tacgagacac ccagaagaag atgatgtcgt aacctgttac
     3241 caaggttgaa gttgggaaat aacgtttaaa gtcttctgag tcgacttcag gccagcccat
     3301 ggttgaaaat ggccagaggg cagaactgaa ccaagtatcc aagacgtctt cgtcctgagt
     3361 ccatccgtca ccttctggag cttcttcgcc gacatacatt tcaccatcag cattgtacca
     3421 ggcagggatt tggtgacccc accaaagctg acgagagata acccagtcgt ggacattttc
     3481 catccattga aggaaggtat cgttgaaacg aggtgggtag aattcgacct tgtcctctgt
     3541 gtcttggttg gcaatggcgt tcttagccaa ttggtccatc ttgacgaacc attgagtaga
     3601 caagcgtggc tcaactacga cacctgtccg ttctgagtga ccgactgaat ggacacgttt
     3661 ttcgattttg acaagggcac cgatttcttc caacttagca acgactgcct tacgagcttc
     3721 aaaacgatcc atgcctgaaa attcaaaggc aagctcattc atagttccgt cgtcgttcat
     3781 gacgttgact tgtggcaagt tatgacgttg gccaaccaag aagtcatttg gatcgtgggc
     3841 aggtgtgatt ttcacgacac cagtaccaaa ctcaggatct gcgtgctcat ctccaacgat
     3901 tgggatgagt ttattagcga ttggaaggat gacgttttta ccaatcaagt ccttgtagcg
     3961 cgggtcttct ggattaaccg caaccgcaac gtccccaaac atagtctcag gacgagttgt
     4021 agcaacttca agggcgcgtg aaccatcttc cagcatgtag ttcatgtggt agaaggcacc
     4081 ttcgacatcc ttgtgaatca cctcaatatc agaaagggct gtgcgagctg ctgggtccca
     4141 gttgatgata aactcaccac ggtagatcca gcctttcttg taaaggtcca caaagacctt
     4201 acgaacagct tttgacaaac cttcgtcaag agtgaaacgc tcacgagagt agtctacaga
     4261 gagccccatc ttgccccatt gttccttgat agtagtggca tattcgtctt tccattccca
     4321 gactttcgtc aagaaagact cacgaccaag gtcatagcgg gaaatgccct caccacgcaa
     4381 gcgctcctct accttagcct gagtggcaat ccctgcgtgg tccatcccag gaagccaaag
     4441 ggtatcaaaa ccttgcatgc gtttttgacg gatgataata tcttgcaaag ttgtatccca
     4501 agcgtgacca aggtgaagtt tacctgtaac gtttggtggt ggaataacga ttgaataagg
     4561 cttagccttt tgatcgcctg aaggcttgaa aacatcagca tcaagccatt tttggtaacg
     4621 accagcctca acctcggctg gattgtattt agatgaaagt tctttagaca tgtgtgttct
     4681 ccttaattac ttttattgac aattttaacc aaaccaattt ttgaaagttc aatataaatc
     4741 tcttcaattt cagcaaatgc tgcaagtggt gacatagtat aatttccagg attatattta
     4801 agatcatact tgcttatctt tgaaatata
//
LOCUS       DAVMAF010000105         4758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMAF010000105 DAVMAF010000000
VERSION     DAVMAF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.106"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            105..1121
                     /locus_tag="VYO97_001939"
     CDS             105..1121
                     /locus_tag="VYO97_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1754684.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKVVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
     gene            1134..1504
                     /locus_tag="VYO97_001940"
                     /pseudo
     CDS             1134..1504
                     /locus_tag="VYO97_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1481..1603
                     /locus_tag="VYO97_001941"
                     /pseudo
     CDS             1481..1603
                     /locus_tag="VYO97_001941"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1589..2212
                     /locus_tag="VYO97_001942"
     CDS             1589..2212
                     /locus_tag="VYO97_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754685.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTIRDSVYLGLNTDYFIETGFASK
                     IQVSEESTFEEDLQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2202..3837
                     /locus_tag="VYO97_001943"
                     /pseudo
     CDS             2202..3837
                     /locus_tag="VYO97_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3940..4632)
                     /locus_tag="VYO97_001944"
     CDS             complement(3940..4632)
                     /locus_tag="VYO97_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1754686.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1480 a         1026 c          821 g         1431 t
ORIGIN      
        1 gtgtactgtt atagattcaa tacactatag aatagaattg tatgttttaa aaacgcttac
       61 attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga
      121 tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag
      181 acggcggttc gctagtcgtt tattcaccaa actcagaggg cttaattgga gcaactattc
      241 ctgcctttga agaaaaatat ggtatcaaag tagaactgat tcaagctggt actggagaac
      301 ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt
      361 cttatacaca atatactacc cacggagaac tctttgaaaa ctatacttca aaagaaaatg
      421 ataatgttat caaagaatat caaaacacaa ctggctactc tactccttat acactagatg
      481 gtagtgtttt aatcgtcaac cctgatttaa ctaaaggcat gaacatcgaa ggatataacg
      541 atcttttcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta
      601 gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg
      661 cttggtctta tgtaaaagat cttttcacac ttattgatgg taaaatcggt tcaagttcat
      721 ctagtgtcta taaagtagtc gctgatggag aaatggctgt tggtctctct tatgaagatc
      781 cagcagttaa actcttaaat gacggagcta acattaaggt agtctatcca aaagaaggaa
      841 ccgtcttcct acctgctagt gctgctatcg ttaaaaaatc taaaaatatg gaaaatgcca
      901 agaaatttat cgattttatt atctctcaag aagtacaaga tacacttggt acaaccacta
      961 ctaaccgtcc tgttcgtaaa aatgctaaaa caagcgaaaa catgaaacca attgacaaaa
     1021 tcaaaacact cactgaagat tatgattatg tcatcaagaa taaatcagat atcgttaaga
     1081 aatacaacga agtctttaca gatatccaat ctaaacagta aaagaggttc actatgagtg
     1141 agatcaaaat tattaacgcc aaaaaaatct accacgatgt ccctgttatt gagaatttga
     1201 acattacaat tccaaaagga agtctcttta cccttcttgg agcttcagga tgtgggaaaa
     1261 cgacccttct tcgtatgatt gcaggtttca acagtatcaa agatggagaa ttttacttcg
     1321 atgatacaaa aatcaataat atggaaccca gcaaacgcaa tatcgggatg gttttccaaa
     1381 actacgctat tttcccacat ttgactgtcc gagacaacgt tgcttttggt cttatgcaaa
     1441 agaagaattg attcaacaga ccaacaagta tcttgaactc atgcaaattg ctcaatatgc
     1501 ggatcgaaag cccgataaac tcagtggtgg acaacaacaa cgtgtcacct tggcatgcgc
     1561 cttagcggtt aatccaagtg ttctcctcat ggacgagcca cttagtaatc tggaggccaa
     1621 acttcgcttg gatatgcgtc aagccatccg agaaatccaa cacgaagtgg gaattacaac
     1681 tgtttatgta acccacgacc aagaagaagc catggctatt tcagaccaaa ttgctgttat
     1741 gaaagatggg gtgatccaac aaatcggccg accaaaagaa ctctatcata aaccagctaa
     1801 tgagtttgtg gcaaccttta tcggacgcac aaatattatc cctgccaatc ttgaaaaacg
     1861 gagcgacggc gcttatatcg tcttttcaga tggctatgcc cttcgaatgc cagctcttga
     1921 tcaggttgag gagcaagcta ttcatgtaag cattcgtccc gaagagttta tcaaagatga
     1981 atctggagat attgaaggaa ctattagaga tagcgtctat cttggactaa atacggatta
     2041 tttcattgag acaggttttg cctcaaaaat tcaagttagt gaagaatcaa cttttgaaga
     2101 agatctacaa aaaggcaatc gtattcgtct acgaatcaat acgcaaaaat taaacatctt
     2161 ttctgcagat ggttcccaaa acctgataaa aggagtcaac catggaacgt aaaaaactaa
     2221 atatttggac agcttcctct ttcttcctct ttcttaccta tcctatcttt ctcgtttatc
     2281 ctatcgttac cgtgctcaag caagcactta tacatgaagg acaattctca ctagctaatt
     2341 ttgtcacttt ctttagtaaa gcctactact ctgagacact agtcaacagt ttcaaggttt
     2401 ccattaccgc tactatcact tccttagttg taggaaccct attagcttat ctcttctcta
     2461 tgtatgactt caaggggaag aaatttctac aaatattgat tatcattgct tccatgtcag
     2521 ctcctttcgt aggagcctac tcctggattc tcttgctggg acgaaatgag gtcatcacta
     2581 aatttttgac aaatgccctt tatcttccag ctatcgatat ttattgattc aaaggaattg
     2641 tacttgtctt tacactgcaa ctattcccac tggtatttct atatgttgct ggaacaatga
     2701 acagtattga caattctcta cttgaagcag ctgaaagcat ggggtccttc ggatttaaac
     2761 ctatcgtaac ggttgtttta cctctcctag ttccaacctt actagcagct ccttgcttgt
     2821 atttatgaga gcattctcag actttggaac gcctatgttg attggcgaag ggtatcggac
     2881 tttccctgtc ctgatttata cccaatttat tagcgaggtt ggaggaaatt ctgcttttgc
     2941 aattatggcg attatcattg ccttggcaat tttccttatc caaaaacaca ttgcaaaccg
     3001 ctacagtttc agcatgaatc tgctccatcc aattgagcct aaaaaaacta caaaaggaaa
     3061 aatggctgcc atttatgcaa cagtctacgg aattatcttt atctctgttt tacctcaaat
     3121 ctacttaatt tatacctctt tcctaaaaac atcaggtatg gtatttgtta aaggttattc
     3181 tccaaacagt tacaagctag ctttcaatcg tatgggatct gctattttca ataccattcg
     3241 tatccctttg attgccttag ttctagttgt tctatttacg acatttatct cctacctagc
     3301 cgttagaaaa cggaatttgt ttacaaactt aattgacagc ctcagtatgg taccttatat
     3361 tgtaccagga accgttctag ggattgcctt catttctctg gtctatttgg aagtggattt
     3421 cttatgatta cagggactgc tttcatcttg attatgtctc tatctgccag aaaattacct
     3481 tatactattc gctcatctgt tgctagctta caacaaatag caccaagtat tgaagaagct
     3541 gctgaaagct taggaagtag tcgtctcaat atctttgcta agattacaac tccaatgatg
     3601 ctatcggata ttatttctgg agccatccta tcttgggtca cactgatttc agaactctct
     3661 acttctatcc tcctctacaa tgtcaaaaca agaacaatga ctgtagctat ttatacagag
     3721 gttctcagag gaaattacgg tgtagccgca gccttgtcaa ctatcctgac tgttctaaca
     3781 gtaggttcct tgctcttgtt tatgaaaatc tctaaaagca atagcattac actttagttt
     3841 tccacatcaa aaacagccga aaggagtacc ctcggctgtt ttttcttatc ttgatattct
     3901 tatcaaatcc ctagctcatg gcaagcaagt ctagactgat taaatagagc cttcttccat
     3961 cttctcacgt cctagctctc ggtaactacg gtcaccgaca acttcaaccc taaactgacc
     4021 gtcctcatat tcaagaatcg tcacgctacc attatctaga ccatgcggat gcatgccatt
     4081 aatcagataa acaatggttc caatagtcat tccatggctg acaacaaggg cgttacctcc
     4141 accttgatct tccatttctt ttgcaatcat ttcaaagcct tccttgattc gaccactgag
     4201 tttttcccag ccttcagccc aaccagctgt atcgacctct accaagccct cagccagttc
     4261 agcataagac aattggtgaa cgtggtccac attaaagata cgaggaataa tgcccatgaa
     4321 aagatcgcca tcataggctc catcaaaact accgaaacac cattctctga tacgcttgtc
     4381 catgcgataa gggattttcc cctgcaagcc aagttcttca aggataattc ccatggtctg
     4441 aatggtacga ccagaatcac tcgaataagc gcgctcaaac tgtagatcag attctcgcaa
     4501 accgattcct aactcttgaa tccctcgttc accttcagca gttaagggag tatcgctcca
     4561 accttgcgcg cgaccaatcg tgttaaacat ggtcttgcca tgacgtacca aatacaatcg
     4621 tacttttacc attttccgtc ctccgtttgc ttctattata tcatgaatga taaaataaaa
     4681 gccacccata caggcgactt ttgaaggaga ttattatgaa aaagtttagg agttcattaa
     4741 ataaagatat tagatgaa
//
LOCUS       DAVMAF010000106         4656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMAF010000106 DAVMAF010000000
VERSION     DAVMAF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.107"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..211
                     /locus_tag="VYO97_001945"
     CDS             <1..211
                     /locus_tag="VYO97_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754687.1"
                     /translation="NMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMVNSNPYAL
                     IYWLALLSIIIPLTVFSVHRNWRRV"
     gene            221..919
                     /locus_tag="VYO97_001946"
     CDS             221..919
                     /locus_tag="VYO97_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754688.1"
                     /translation="MGKLSSHIWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            916..1563
                     /locus_tag="VYO97_001947"
     CDS             916..1563
                     /locus_tag="VYO97_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754689.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1643..2508)
                     /locus_tag="VYO97_001948"
                     /pseudo
     CDS             complement(1643..2508)
                     /locus_tag="VYO97_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(2520..3284)
                     /locus_tag="VYO97_001949"
                     /pseudo
     CDS             complement(2520..3284)
                     /locus_tag="VYO97_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3285..3572)
                     /locus_tag="VYO97_001950"
     CDS             complement(3285..3572)
                     /locus_tag="VYO97_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1754690.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(3582..3962)
                     /locus_tag="VYO97_001951"
     CDS             complement(3582..3962)
                     /locus_tag="VYO97_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1754691.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(3922..4602)
                     /locus_tag="VYO97_001952"
     CDS             complement(3922..4602)
                     /locus_tag="VYO97_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1754692.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         1392 a          795 c         1009 g         1460 t
ORIGIN      
        1 aaacatgagc ttagtatttc tgtttacttt tttatttgta gaatccttat tctggattta
       61 tcagttgaac aatgggataa ttggattatt gccaattttt cagtatatgg taaattccaa
      121 tccgtatgca ttgatttatt ggcttgcatt actatctatc ataattccat tgactgtatt
      181 ttctgttcat agaaactgga ggagagtgta aaagttggaa atgggaaagt taagtagtca
      241 catatggagg ttgaatcaga taatctatac caagtacttt tggggttatg ttcttttttg
      301 gatattgatt tgtttaggat tatggtattg gttagaagga aatgatagac ttgttataga
      361 aattttaaaa gggcctaatc tgagtcaaaa ctctttttta gtcttatcta tatggttgct
      421 tcattggttt attattcata cattttttct agcagttgta tatcgtagaa gagcatccga
      481 tttctttatg gaagtgattc gattttcttc tattaagctc tggattaggt atcagatttg
      541 gacctgtttt ctttatggac tcattttaat catggtaaaa gttctagtga ttcaatttat
      601 gttacagtta gcaaactggg atataggagt tttgtttata gttgattctt tgaatgcttg
      661 tgtgctatcc ttgctttgct ttatgttata cgcactagga gcgaatgtac aaatgaactt
      721 tgcttgcgtt agtttctttt tactcatgat tgtgtttggt ggtctatttg taggaaatcg
      781 aactaattat ctattttata ttttgaatcg agggaatgga gatataggac gagatttatt
      841 tcttcagttg ctatttttag tctttctttt taaaagcatt ttctatttca ctcgtcaaaa
      901 gaggaggttt atagaatgaa tgaaataatt acattaaaaa atattgagtt gaaattaaaa
      961 aaaacatgtg tttttcaaaa ccttaatttt agttgtaaac agggggaaat tataggaatt
     1021 actggtgcga atggctcagg taaaagtgta ttgtttaaat taatagctgg tttatatagt
     1081 ccgtcttatg gagaagtgtt aatcaatggg gaaaatattg ttcctgagag aaaaattcca
     1141 gctaatttgg gagctttgat tgaagaacct ggttttataa attattatag tggctttaag
     1201 aatttacaat atttggcaag catacgagga gtagttggta atcaggaaat caatgataca
     1261 ctgaaaatag ttggtctata tgagcaaaaa gaccagaaag ttaaaactta ttcgctaggt
     1321 atgaggaaaa agctagggat tgctcaagca attatggaga atccctctat tcttttacta
     1381 gatgaaccta tgaatgcctt ggataaatca agtgtagaaa atatgagaac attgtttaga
     1441 aagctctcta gtgaaaaagg gacaacaatt ttgattgcta gtcatagtga agaggatatt
     1501 cgtatcttat gtgataaagt atatgcaata gaagataaag tatgtacact gtgttcagat
     1561 tgaacatctt agatagactc aggatgttat aggtaaaatc ttaaaaaggg attggagctt
     1621 aaaatccagt cccttttaag agttaatggc caccttcaac aaccaggtca tctgctttag
     1681 cagcgtcacc gtaggttggg tgaagtgttt tttcataggc cttgtggaag aagttttcct
     1741 tgcctaattt ttcaatatct ttattgatga agtctagcaa ttcttggttg cctttttgaa
     1801 ctgctgccgc aatggtatcg ggatcaccga gggaagtaat tcctacttca aatcctttat
     1861 tttcaagtgc ccaagctaga acttccgtat tgtcagttga aaaggcatct ccacgtccgt
     1921 caagaagagc ttggtaagag tcactgtatt ggtcgtattt ttggagtttg atttctggat
     1981 gattcttttc aaaataagtc tcagcagtcg ttccttttgt gacaattaag gttttacctt
     2041 caagttgttt gacgtctgta atgagaccag tcttaggtga tacgacaccc agagaaactt
     2101 tcatatatgg aagggcaaaa tcaacttgtt tcttacgttc gtcagttact gtaaagttag
     2161 caagagtaat atctaccttg tttgaaatca agtattccgc acggttggca gcatcgactg
     2221 aaatgtattt aaccttgaca ccaaggtctt gagctagttg gttccctagt tcaatatcgt
     2281 agcgtagcct tggtaagaac catcattgtc aacgtagcca aacggttttt tatctccaaa
     2341 cacggcgatt cgcagttcac cgcttttttt gatttcatcg atagtgcgag ctttggcagt
     2401 tgtttttcca gaggatgaac cagcgtttcc acctgagcta caagcagtga taaagataag
     2461 ggcaaaggca agtgctaaaa cagttaagag tggtttgaat agtttcataa gaatctcctt
     2521 tatagatatg agccgaattg gctaaagtca aagacgttta aaaattcctg ggctcgtttg
     2581 gtttgcggat tggtaaagaa ggcttgagct gttccttctt cagcgatttt cccttggtcg
     2641 aggaagataa tccggtcagt aatggcttgg gcaaactgca tttcgtgggt tactaaaatc
     2701 atggtacggc cttcttgggc caaatcattg ataagttcca gcacctcacg caccatttct
     2761 ggatccagcg aagcagtcac ctcgtcaaaa aggatgattt ctggatgcat taggagggca
     2821 cgggcaattg caacacgttg cttctgtcca ccagataatt gacgggcaaa gcaaagctat
     2881 gttgtttatc cagcaaatcg acacgatcta gtaattgcaa agcttcttcc gttacttctt
     2941 tcttgtccct tccttgagct ttgatagggc ctaggatgag gttttgtaag acatccagat
     3001 ggggaaagag ttcataactt tgaaagacca tgccaatctt ttggcgaact aggtgaaaat
     3061 cttttttatt ttcaacgata gactgaccat ccagaagaat atctccacct tgaatacttt
     3121 ctaagccgtt gaggcaacga aggagggtac ttttcccaca accagatgcc cctaggatga
     3181 caacaacttc cccctttttt gatttctaga gaaagtcctt ggaggatggg attgtctccg
     3241 aaggattttt ttagttcctt gatttctaag atagtttcag acatttagtt tctccaatgt
     3301 ttttctaagt gagtggatag tttggaaata gggtagcaaa ctgcgaaata taagactaga
     3361 atggttccat aaatccaaaa tgaagcagtt gggatggtca ggcgattgct atcgatgatt
     3421 tgttgtccaa ctttggtcac ttccacaacc ccaatcaaaa caactaatga agtggtttta
     3481 atcatccgag tgacaagatt gatagcctgc ggtagcagtc ttcttaagac ttgtgggatg
     3541 atgatgtggt agtaaagttg aacattagtc aagtcgagtg cctatccact ttcaaactga
     3601 tgtttaggga gagaagtgat agctccacgt accaagtctc ccatttcagc tgttccccag
     3661 agggtaaaaa tgataatagc tgaagtctca cctgagatat tgatattaaa gtttcgagcc
     3721 aagccaaagt aaacgatgaa gagtagcacc agctggggca tgatacggat aaattccaga
     3781 tacaatcgtg ttaaaaatcg tatgattcta gaatgggagg tcatgatgat tcccatgact
     3841 gttccgaaca tcatggataa gaggacagac aggatggata tcccaatcgt aacgcccaac
     3901 ccctgtaaga ttctcaggag attatttcct tgaaagagta cttggattcc cgaatcctgc
     3961 atggcgggtc ctcctttcta tccagctaaa gaccagtgag atgggtagca gcatgatcag
     4021 ataagcaact accaacatag ctagcgcaat gtctgtctca tagtagagac caatcaaatc
     4081 cttggcgacg tacatgaggt cggccaaagc cactgctgag aaaacagagg tttccttgat
     4141 aaggaaaatg acattggcac taaaggaggg gagtgccacc gctgttgctt gcggaagaac
     4201 cacatagcga aagacctgta gaggtgtcag accaatagcg aggccaatct cctgctgggt
     4261 ttgactgatg gcttccagcc cacttcggaa agattctgcc atataggagc ctcctaaaaa
     4321 gacaaggcca agcgttgcac agacttctga agataggaca atcccgattc ggggaagacc
     4381 gaagtagaga aagaagagtt gaatcaaaag gggcgtatta cgtgacaatt caatgtaggc
     4441 tgtcgctact tgcgccaaaa cagggattcg ataatgtcgg atgatactaa cgattaaacc
     4501 gagcagaaag gatcccaaaa ttccccaaac tgcaatatgc aaggtcagaa agaatgcctt
     4561 ttgatatagt ggtagatatt gttcaacaat ggaccaatcc aaaaatagaa cctcccatct
     4621 agaaataata cagttattgt agcacttaaa atcttc
//
LOCUS       DAVMAF010000107         4554 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMAF010000107 DAVMAF010000000
VERSION     DAVMAF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.108"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            19..1410
                     /locus_tag="VYO97_001953"
     CDS             19..1410
                     /locus_tag="VYO97_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1754693.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            1553..2470
                     /gene="adcAII"
                     /locus_tag="VYO97_001954"
     CDS             1553..2470
                     /gene="adcAII"
                     /locus_tag="VYO97_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1754694.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            2478..>4554
                     /gene="phtD"
                     /locus_tag="VYO97_001955"
     CDS             2478..>4554
                     /gene="phtD"
                     /locus_tag="VYO97_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1754695.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHP
                     ERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWI
                     KKDSLSEAERAAAQAYAKEKGLTPPSTDHQDLGNTEAKGAEAIYNRVKAAKKVPLDRM
                     PYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHP
                     NERPHSDNGF"
BASE COUNT         1376 a          867 c          975 g         1336 t
ORIGIN      
        1 tttattttgg agtcaaagat gaatatattt agaacaaaga atgttagttt agataaaaca
       61 gagatgcata ggcatttgaa gttatgggat ttgattttgc tgggtatcgg agccatggta
      121 gggacaggcg tctttacaat cacaggtact gcagctgcaa cacttgctgg cccagcccta
      181 gtgatttcaa tcgttatttc tgccttgtgt gtgggattat cagccctctt ttttgcagaa
      241 tttgcctcgc gagtacccgc tacaggaggt gcctatagtt acctctatgc tatcttagga
      301 gaattccctg cctggttggc tggttggtta accatgatgg agttcatgac agccatatca
      361 ggcgtagctt cgggttgggc agcttatttt aaaggcttgc tctctcaata cgggatagcc
      421 tttcctcagg ctttaaatgg tacctttaat cctcaagcag ggacatttgt tgatttattg
      481 cctattcttg tcttggtctt ggtgacttcg cttgttttac taaatgccaa ggcagcctta
      541 cgctttaatt cgattctagt tattttgaaa ttttccgctt tagctctctt tgttttagta
      601 ggaatttgga atattaagtt tgataattgg agcaattttg ctccttacgg ttttggacaa
      661 atctatggag ctagcactgg tattatggct ggtgcgtcct tgatgttctt cggttttttg
      721 ggatttgaat ccatctctat ggctgtagat gaagtcaaga ctcctcaaaa aaatattcct
      781 agagggattg tcttatcgct ttctatcgta accattctct atgccttggt gacccttgtt
      841 ttgactggtg tggttcacta tagtcattta aatgtcgatg atgccgttgc ctttgccctt
      901 cgtagtgttg ggattagttg ggcagccaac tatgtgtcat tagtggctat cttgaccttg
      961 attacagttt gtatttcgat gacctatgcc ctatcgcgta tgatttacag tttagcacgt
     1021 gacggcttag tgcctgctgc ctttaaggaa ctgacgaaga ctagcaagat accaaagaat
     1081 gctactatct tgacaggtct agcttcagca gtagcagcag gaatgttccc actagccagt
     1141 attgcagcct ttttaaatat ttgtacctta gcctacttga tcatgttggc ttatggtctg
     1201 attcgcttac ggaaagaaaa aggaatgccc aaagctgggg aatttaaaac accattggta
     1261 cctctgttac caattttatc gatcatcatt tgtttatcct tcatgttaca gtataatatg
     1321 aatacctgga ttgctttcct agttgcgttg ttagtaggaa gtattattta cttcacttat
     1381 ggctataagc attctaccat agaagaataa agtgaaagtc tattgagttg ttgacactca
     1441 gcagattttt atatgtgaaa gaaatttcaa attttttcta aaaatatctt gacagattaa
     1501 attttcagga gtagaatatt tactagttaa ttaaatggtt aaggagttgt tcatgaagaa
     1561 acaaaattta tttttagtcc tgttaagtgt ctttcttttg tgcttggggg cttgtggtca
     1621 aaaggaaagt cagacaggaa aggggatgaa aattgtgacc agtttttatc ctatctacgc
     1681 tatggttaag gaagtatctg gtgacttgaa tgatgttcgg atgattcagt caagtagtgg
     1741 tattcactcc tttgaacctt cggcaaatga tatcgcagcc atctatgatg cagatgtctt
     1801 tgtttaccat tctcatacac tcgaatcttg ggcaggaagt ctggatccaa atctaaaaaa
     1861 atccaaagtg aaggtcttag aggcttctga gggaatgacc ttggaacgtg tccctggact
     1921 agaggatgtg gaagcaggag atggagttga tgaaaaaacg ctctatgacc ctcacacatg
     1981 gctagatcct gaaaaagctg gagaagaagc ccaaattatc gctgataaac tttcagaggt
     2041 ggatagtgag cataaagaga cttatcaaaa aaatgcgcaa gcctttatca aaaaagctca
     2101 ggaattgact aagaaattcc aaccaaaatt tgaaaaagcg actcagaaaa catttgtaac
     2161 acaacataca gccttttctt atctagcgaa gagatttggg cttaatcaac ttggtattgc
     2221 aggtatctct cctgaacaag aaccaagtcc acgacaacta acagaaattc aggaatttgt
     2281 taagacctat aaggttaaaa cgatttttac agaaagtaac gcttcttcaa aagtagctga
     2341 aactcttgtc aaatcaacag gtgtgggtct taaaactctg aatcctttag agtcagaccc
     2401 acaaaatgac aagacctatt tagaaaatct tgaagaaaat atgagtattc tagcagaaga
     2461 attaaagtga ggaaagaatg aaaatcaata aaaaatatct agcaggttca gtggcagtcc
     2521 ttgccctaag tgtttgttcc tatgaacttg gtcgtcacca agctggtcag gttaagaaag
     2581 agtctaatcg agtttcttat atagatggtg atcaggctgg tcaaaaggca gaaaatttga
     2641 caccagatga agtcagtaag agagagggga tcaacgccga acaaattgtt atcaagatta
     2701 cggatcaagg ttatgtgacc tctcatggag accattatca ttactataat ggcaaggttc
     2761 cttatgatgc catcatcagt gaagaacttc tcatgaaaga tccgaattat cagttgaagg
     2821 attcagacat tgtcaatgaa atcaagggtg gctatgtcat taaggtagac ggaaaatact
     2881 atgtttacct taaagatgcg gcccatgcgg acaatattcg gacaaaagaa gagattaaac
     2941 gtcagaagca ggaacgcagt cataatcaca actcaagagc agataatgct gttgctgcag
     3001 ccagagccca aggacgttat acaacggatg atgggtatat cttcaatgct tctgatatca
     3061 ttgaagatac tggtgatgct tatatcgttc ctcacggcga ccattaccat tacattccta
     3121 agagtgattt gtctgccagt gaattagctg ctgcccaagc ctattggaat gggaagcagg
     3181 gatctcgtcc ttcttcaagt tctagtcata atgcaaatcc agctcaacca agattgtcag
     3241 agaaccacaa tctgactgtc actccaactt atcatcaaaa tcaaggggaa aacatttcaa
     3301 gccttttacg tgaattgtat gctaaaccct tatcagaacg ccatgtagaa tctgatggcc
     3361 ttgttttcga cccagcgcaa atcacaagtc gaacagccag aggtgtagct gtccctcatg
     3421 gtaaccatta ccactttatc ccttatgaac aaatgtctga attggaagaa cgaattgctc
     3481 gtattattcc ccttcgttat cgttcaaacc attgggtacc agattcaaga ccagaacaac
     3541 caagtccaca atcgactccg gaacctagtc caagtctgca acctgcacca aatcctcaac
     3601 cagctccaag caatccaatt gatgagaaat tggtcaaaga agctgttcga aaagtaggcg
     3661 atggttatgt ctttgaggag aatggagttt ctcgttatat cccagccaag gatctttcag
     3721 cagaaacagc agcaggcatt gatagcaaac tggccaagca ggaaagttta tcgcataagc
     3781 taggagctaa gaaaactgac ctcccatcta gtgatagaga attttacaat aagacttatg
     3841 acttactagc aagaattcac caagatttac ttgataataa aggtcgacaa gttgattttg
     3901 aggctttgga taacctgttg gaacgactca aggatgtctc aagtgataaa gtcaagttag
     3961 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
     4021 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
     4081 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
     4141 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
     4201 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
     4261 atcaggattt aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
     4321 cagctaagaa ggtgccactt gatcgtatgc cttacaatct tcaatatact gtagaagtca
     4381 aaaacggtag tttaatcata cctcattatg accattacca taacatcaaa tttgagtggt
     4441 ttgacgaagg cctttatgag gcacctaagg ggtatagtct tgaggatctt ttggcgactg
     4501 tcaagtacta tgtcgaacat ccaaacgaac gtccgcattc agataatggt tttg
//
LOCUS       DAVMAF010000108         4484 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMAF010000108 DAVMAF010000000
VERSION     DAVMAF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.109"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            136..2316
                     /locus_tag="VYO97_001956"
     CDS             136..2316
                     /locus_tag="VYO97_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1754696.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VYO97_001957"
     CDS             2368..3183
                     /locus_tag="VYO97_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1754697.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAVLNWK"
     gene            3388..>4484
                     /locus_tag="VYO97_001958"
     CDS             3388..>4484
                     /locus_tag="VYO97_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465984.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1754698.1"
                     /translation="MFFQDRKIKYSIRKLSVATVSLAIGFLFAPAALGVAQVAQADEL
                     STTEQVENKAENQVSVVSFATEQEESETTYVDTTNKVTWTFAGYDKEKIVVGKGRQTF
                     LGSWVPTPNPEYVFKSSKAGVPLPQSILGMLPKDEASYKVGDTIVAKQPAVESVVEEE
                     KDYVWTFKGYDQKNATYNGKRVTFTGIWEVTPRPHHVSYTFVSVTSGVDLPKFIQKKA
                     PKDGSTYFNRTRVFAEEPTTKTYIDSVNDGTWTFKGYDAASAVVNKANVEFVGKWEFK
                     ANPTNAETYTPQVTEETIKVGQTPDLTDNVTNLPNLPAGTKVVDITPAGQIDTTKPGT
                     YTGKVRVDYPDGSSTEVSVSVNVLPAPETQT"
BASE COUNT         1381 a          819 c          978 g         1306 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaagtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttgcaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatctttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 gctagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agtgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgacgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtcatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaagat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttgctttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgta tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttac agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccatgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactgtagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgcggtatt aaattggaaa
     3181 taaaatcaaa aaatgaaagg gagcgagtcc tttcattttt ttacaaaaat ccctataatt
     3241 actattttgg agaaggagtt ttttgtatat ttgtatatta tgaggaaata tccacaaata
     3301 agtttttaaa actttacggt caaaaaaaat cgctaatatt aactatttat gatagaatga
     3361 aaattatgaa ataaaaggag gactggaatg ttttttcagg atagaaagat aaaatattca
     3421 atccgcaaat tgtcagttgc tactgtgtcc cttgcgattg gatttttgtt tgcgcctgcg
     3481 gcgcttggag tcgctcaagt agctcaagct gatgagcttt caactactga acaagtggag
     3541 aataaagcag agaatcaggt ctcggtggtc tcttttgcaa cagagcaaga agaaagtgaa
     3601 acgacttatg ttgatacgac gaataaagtg acttggacat ttgcaggata cgataaagaa
     3661 aaaattgttg taggaaaagg cagacagact ttccttggaa gctgggtacc tactccaaat
     3721 ccagaatatg tattcaaatc atctaaagca ggtgttccat taccacaaag tattttggga
     3781 atgcttccaa aggatgaagc ttcttataag gttggtgaca ccattgtagc caaacaacct
     3841 gctgtagaat cagtagtcga agaagaaaaa gactatgtct ggaccttcaa aggttatgat
     3901 cagaagaatg cgacgtacaa tggcaagcgt gtgacattta caggtatctg ggaagtaacg
     3961 cctagaccgc atcatgtaag ctatactttt gtaagtgtta catctggtgt tgatttgcct
     4021 aaatttatcc agaagaaagc accaaaagat ggctcaactt acttcaatag gacgagagtt
     4081 tttgcagaag agccgacaac aaagacctac atagatagtg tgaacgatgg cacatggacc
     4141 ttcaagggtt atgacgcagc tagcgccgtt gttaacaagg ctaatgtaga gtttgtaggt
     4201 aagtgggaat tcaaggcgaa tccgacaaat gcggaaactt atacgcctca ggtaacggaa
     4261 gaaactatca aggttggaca aacacctgac ttgaccgata atgtgacgaa cttgccaaac
     4321 cttccagctg gcactaaggt agttgatatt acacctgcag gtcaaattga cacaacaaaa
     4381 ccaggtactt acacaggtaa ggtgcgagtt gactatccag atggttcttc tactgaggtg
     4441 tctgtatcag ttaatgtttt gcctgcacca gaaactcaaa ccta
//
LOCUS       DAVMAF010000109         4317 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMAF010000109 DAVMAF010000000
VERSION     DAVMAF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.110"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(39..1127)
                     /locus_tag="VYO97_001959"
     CDS             complement(39..1127)
                     /locus_tag="VYO97_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1754699.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1145..1813)
                     /locus_tag="VYO97_001960"
     CDS             complement(1145..1813)
                     /locus_tag="VYO97_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1754700.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1832..4270)
                     /locus_tag="VYO97_001961"
     CDS             complement(1832..4270)
                     /locus_tag="VYO97_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1754701.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
BASE COUNT         1352 a          904 c          659 g         1402 t
ORIGIN      
        1 taaaagacta ccatttggaa aacagtagtc cttattgttt attttgaatt tacataggca
       61 tctacagcga taatagcttg agcaacatct gaaggcgaaa tcttaaacgg catctgatga
      121 attgtctcac cctccatagt tgcttgttta ccaactttta ttaaatcatc atatccaact
      181 tgatccaaat gcatttcttt tagagttgtt ggcataccaa tttttttgta aaactcaata
      241 tacttatcaa gttcttcttt aggtctattt tccaataata gttgtactaa agttccataa
      301 gctacttttt caccatgtgt taaatgatga atgtcacctg tcaatgcagt aaaaccatta
      361 tgaattgcat gcgccgcagc taatcctcca ctttcaaaac ctaaaccact caataaagta
      421 ttagcttcaa caatgttttc taatgctggt gtcaccactt tagcttcaca agctgccata
      481 gcctgtaaac catctgcaaa cagcgtttct tcacatttct tcgcaattgc aactccagcc
      541 aatgtttgct gttgtcccaa catagttttt ccatttgcct gcataaccgc acgcgcctca
      601 acccaagttg ctaaaccatc tgcaatacca gacgctaata aacgcttagg ggcttgtgaa
      661 ataacttttg tatcaaccaa aactaaatct ggatttttag aataaaatag ataatgatca
      721 aatgcacctt catctgtata aataacagat aaagcagata caggtgcatc ggtcgatgca
      781 attgttggag caataataac aggtttttca atcaaatctg caatagcttt tgcgctatca
      841 atcgtctttc ccccaccaag accgataata ctatcacaat tttctttctc agccaaggca
      901 acaactcgat tgatttcatt gtcagaagct tcaccattaa atagcgccag aacaatatgg
      961 aaaccatacc tatgtaggta atcttcaaat cgttttccaa caatatcata aaccaactga
     1021 tcgcataata gaatcgggta atttcccaaa tccaaaattg atttggcatt ttcaaacaag
     1081 gcattttccc cctgaatata tctagaagga ctagcaaaaa ttctcatatg tatttcctct
     1141 ctatctaaat ggaacgacta ttttgaataa caaaccaatc gtcagaaaaa tcatcaaccg
     1201 ccttttggat agatggcatg gcgaaagctg attcaaaaac atctgctcct gctgtaacag
     1261 catgcgcacc tgcagctaaa gcattattta cttgtgctac atttttaaag gatgcagcta
     1321 aaatcttact aggagagttc tgtctatcaa tagcaagagc taattgacga atgacagaat
     1381 ttgaatcaat gttcagattt tccattctat tataatatgg agctaggtaa tccgctcctg
     1441 cttcgatagc taataatccc tgaataactg tataaatagc tgttgcagtg atatggtagc
     1501 cctctttttt tagcaccttt attgcacgta atccagccgg agtaacaggt actttgataa
     1561 atatatcatc tcctgcttgt cttcgaattt catgagcatc ctttaagatg ccttcaaaat
     1621 cttgagaaat cacctgaaca tgaatagagg gtgtagagcc aatcaattct cttacatctt
     1681 tgattcgttc aaaaaaatta atagaaccct ctctttttgc aatagtggga tttgaagtta
     1741 ccccagctag cggcaaaatt tcagaccact ttttaatctc atctaaattt aatgtgtcaa
     1801 gcataaattc cataaaaaga acctctttat tttacaatgt atgctcagta cgtcctataa
     1861 tgtcatcttg ggttgcctta gaaagaacat tgaagaatgc agagtatcct gcaacacgaa
     1921 caattaagtc tctgtgtttt tcaggatgtt tctgagcgtc aatcaacgtc tctctggaaa
     1981 caacattgta ttgaatatgg tacccatgta aacgattaaa gaatgttcgt aacaaagcaa
     2041 ttagttttaa tttatcttct tctttggcta acgtttgagg atttactttc tgatttaaga
     2101 gaaccccacc tacgatttca tctgttggta attttgaaac agattttaaa acagatgtag
     2161 ggccgtgttg atccatatta tgtgatggtg aacaaccctc tgctaacggt gtacccgcgt
     2221 tgcgtccatc tggagttgct aatgttccac gtccctgccc tacgttggct gagatagaag
     2281 atgttcctga ataacgaatt cctccaatag gccctcttcc ataacgtgta ttaggatatt
     2341 tagcaatttc atcaacataa atgtcataag cagcagtaac caatttgtca gcataatcat
     2401 catcattacc atacttaggt gcatcatgaa tcaacatttc ttgaatgacc ttaccttctt
     2461 ctccggcata atctgtttcc agtgcatgcc aaagctgact tgggcttata cgttcttcct
     2521 caaacaccaa ttttttaatt gcagctaacg aatccgacaa atttgcaatt ccaacttgca
     2581 atcctgatat ataatcatat actgctccac cttctttaag gtgttttcca cgaccaatac
     2641 aatcatcagt caatgctgaa catagaatat caggaacttc tcgttccaat gataaatcaa
     2701 tagaattttc aacaataaca ctcattcgtg tcaaatatct tagtgtttta tcccaagcat
     2761 tttctaattc agaaaagttc ttcatatcct taaaatgacc aaagcttggt gcaaaccgtt
     2821 tacccgaagc cggatcaatt ccatcattca tcgtgataag tagaacctta gggaagttca
     2881 tataactcat acctgtgcaa cgatagcccc atttccctgg aactgccgtt tcaacacatc
     2941 caatggcact gtaatcataa gcatcatctt ccaatactcc ttttgcaata aaagaaggaa
     3001 taataatctc atcattatta aatgcaggca taccaaaacc aagtttcatc acttcaatac
     3061 actcattcat gaaacgagca tctaaacctg catggtaacg tacagttaga ttaggttgcg
     3121 gtagatgggt ttgtgcaact gattttaata ccaaataaga taatgggtta acagcatcct
     3181 tcttatctcg agtctgtcca ccaattgtaa cattttgata taaaggactt cctgctgaag
     3241 aaaatgtatg tgattgactg cgaaccttat taattgtaat tgtcttaatc caaagatttg
     3301 tcagacgttc aacaatgcta tcttctgttt ctttaccact ttctaaatca gccttcatat
     3361 atggatacat atattgatca aaacggccat atgaaagaga gtggccatta gattcaattt
     3421 gtaaaataca ttgaataaac caaactgatt gaatagcttc tgcaaaagta gttgccggtt
     3481 catatgggac tctagagcaa atatctgcaa tctcaagtaa ttctttctta cgtttaggat
     3541 ttgcattttc ggctaaactt ttagcaagag caacaaagcg ctttgcatat actttaatag
     3601 catcgattac gataaatata gagtcgtaaa aatgatattt atcaatactt gctggatctg
     3661 ttaaatctag agctactttt gctttacgag cccgctcttc aaaacctctt aaaccaaatt
     3721 gcaaaagttt ctgatagtta actgctaagt gagcatctcc agaattcatc ttaccttcca
     3781 taccgaagaa tcctgtttcc atataaacag acacttcttc aggtaataag gcaccagctc
     3841 tagcacgtaa attattattt tcccaaaacg gagcaatact tctaagttgt tcttttgttt
     3901 cttctgtaat atagaaaaca tctccatcac gcttttcaaa aagatccaac tcattgagaa
     3961 caaattctag cgtatattcc ggaaaaatag gagcatcttt attggaagaa gcttgatttc
     4021 ccgcaatcat agattcttct tcaatataga tagacatatt ttccaaaatt tctttcagca
     4081 tatatgcacg ttttaggaca ttaggttgtt ccttgtatcg ttcataggcg cgtgttgcta
     4141 gaactgctct ctcagcatca atataaggtt ttttatttaa aacatcttct cgatatttgt
     4201 tcatcctttc agtaaggctg ccaaaatatt ctgtcttgat tgttgttctt gctacttctg
     4261 tatttaccat cgtaagcacc tttctttcga aaatattttt atttctttcg aaaatat
//
LOCUS       DAVMAF010000110         4316 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMAF010000110 DAVMAF010000000
VERSION     DAVMAF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.111"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            85..1260
                     /gene="mutY"
                     /locus_tag="VYO97_001962"
     CDS             85..1260
                     /gene="mutY"
                     /locus_tag="VYO97_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1754702.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNIMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1315..2019
                     /locus_tag="VYO97_001963"
     CDS             1315..2019
                     /locus_tag="VYO97_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1754703.1"
                     /translation="MKKILIVDDEKPISDIIRFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2012..3361
                     /gene="vicK"
                     /locus_tag="VYO97_001964"
     CDS             2012..3361
                     /gene="vicK"
                     /locus_tag="VYO97_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1754704.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     LLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3363..4172
                     /locus_tag="VYO97_001965"
     CDS             3363..4172
                     /locus_tag="VYO97_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1754705.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT         1292 a          762 c         1001 g         1261 t
ORIGIN      
        1 gatagaaaag ttatatacta tctattgaaa atattataaa tcagatacaa agacaggtga
       61 aaccttctgt gttatactag agatatgtta gatttgaaag aatacggtat cgtcatgtgg
      121 ccggaggaga aagtcatttc tttccgtgag aaacttctcg cttggtatga tgaaaacaaa
      181 agagatttgc cttggaggag aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg
      241 cttcagcaga ccagggtgga tacagttatc ccttactacg aaagattcct ggactggttt
      301 ccaactgtcg aaagtctggc aactgcgtct gaggagagtt tactgaaagc ttgggagggc
      361 ttgggctatt attctcgagt tcgcaatatg caggctgcag cccagcagat tatgactgac
      421 tttggtggcc aatttccaaa tacctatgaa ggaatttcca gcttgaaagg gattggacct
      481 tacacagcag gagccatttc cagtattgct tttaacttgc ctgagccagc tgtagatggt
      541 aatatcatgc gggtcttggc gcgtctgttt gaagtcaacc acgatattgg gattccaagt
      601 aatcgaaaaa tttttcaggc aatgatggag atcttgatta atccggatcg tccgggtgat
      661 tttaatcaag ccttgatgga cttagggtct gatattgagt ctcctgtaaa tcccagacct
      721 gaagaaagcc cagtcaagga ctttagtgcg gcatatcaga atggaaccat ggaccgttat
      781 ccaattaaat ctcctaagaa aaagccggtt cctatttatc ttaaagcctt agttgtgaaa
      841 aattctcaag gacaattttt acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt
      901 tggcatttcc cctttataga agttgataac ttttcgcaag aagagcagtt tgacctcttt
      961 catcaggttg cggaagaaag tgtgaacttt ggccccagtc cagaagagag tttccagcag
     1021 gactatgacc tagatgttga ttggcttgat gtttgttttg atacagtcca gcatgttttt
     1081 agtcatcgaa aatggcatgt acagattgta gcaggtcagg tgagtgactt ccatgatttt
     1141 tcagataggg aagttcgttg gctttcacca gaagaattca agaattaccc acttgccaaa
     1201 ccccaacaaa aaatctggca ggcttatgca caagccaact tagacagtag ccaagactag
     1261 ctattgtgtc ttctgactat tttttggtat aatagtagag aaaaaggtga acatatgaaa
     1321 aaaatactaa ttgtagatga tgagaaacca atctcggata ttatcaggtt taatatgacc
     1381 aaggaaggtt acgaagttgt aactgctttt aatggtcgtg aagcgctaga gcaatttgaa
     1441 gcagagcaac cagatattat tattctggat ttgatgcttc cagaaattga tggtttagaa
     1501 gttgctaaga ccattcgtaa gacaagcagt gtgcccattc ttatgctttc agccaaagat
     1561 agtgaatttg ataaggttat cggtttggaa cttggggcag atgactatgt aacgaaaccc
     1621 ttctccaatc gtgagttgca ggcgcgtgtt aaagctcttc tgcgtcgttc tcaacctatg
     1681 ccagtagatg gtcaggaagc agatagtaaa cctcaaccta tccaaattgg ggatttagaa
     1741 attgttccag acgcctacgt ggctaaaaaa tatggcgaag aactagactt aacccatcgt
     1801 gaatttgagc ttttgtatca tttagcatcg catacaggtc aagtcatcac gcgcgaacac
     1861 ttgcttgaga ctgtctgggg ttatgactat tttggtgatg tccgtacagt tgatgtgact
     1921 gtacgacgtc tgcgtgagaa gattgaagat acgcccagcc gaccagagta tatcttgacg
     1981 cgccgtggtg tagggtatta catgagaaat aatgcttgat ttactgaaac aaaccatttt
     2041 taccagagat tttatcttta tcctgatttt gttaggtttc atccttgttg tgaccctctt
     2101 attactggaa aatagacgtg ataatattca gttgaagcaa atcaatcaaa aggttaaaga
     2161 tttgattgca ggagattatt ccaaggttct tgatatgcaa ggtgggtctg aaatcaccaa
     2221 tattaccaat aatttgaatg acttgtcgga ggttattcgt ctcactcagg aaaatctaga
     2281 acaagagagt aagaggctaa atagtattct gttttatatg acagatgggg ttcttgcgac
     2341 caaccgtcgg ggtcagatta tcatgattaa tgatacagcc aagaagcaac tggggttggt
     2401 taaggaagat gttctgaata gaagcatttt ggaattgctc aagatagaag aaaactatga
     2461 attgcgtgat ttgattaccc aaagtccaga attgttgcta gattcccaag atatcaatgg
     2521 cgaatatttg aaccttcgag ttcgctttgc cttgatacgt cgagagtctg gctttatttc
     2581 aggtttggtg gctgttttgc atgatacgac ggagcaggag aaggaagaac gagaacgaag
     2641 actctttgtt tccaatgtta gccatgagtt acggactcct ctgactagcg taaaatccta
     2701 tcttgaagcc ttggatgagg gggctttgtg tgaaactgta gcaccagact ttatcaaggt
     2761 ttctcttgat gagaccaacc gtatgatgcg catggtgacg gatctcctcc atctttcacg
     2821 tattgataat gctaccagtc tcctagatgt ggaactgatt aacttcactg cttttattac
     2881 ctttatcctc aatcgttttg acaagatgaa aggacaggaa aaggagaaaa aatatgagtt
     2941 ggtgagagat tatcccatca attctatctg gatggaaatt gatacagata agatgacgca
     3001 ggttgtcgac aatattttaa ataatgctat taagtattcg ccagatgggg gtaaaatcac
     3061 tgtcagaatg aagacaactg aagaccagat gattttatcc atttctgacc acggtttggg
     3121 gattcctaag caggatttac cacgtatctt tgaccgtttc tatcgtgtgg atcgtgctag
     3181 aagtcgtgca caaggtggta caggtctagg actgtctatc gctaaagaaa ttatcaaaca
     3241 acataagggc tttatttggg ccaagagtga atacggcaag ggttcaacct ttaccattgt
     3301 gctcccttat gataaggatg cagtgaaaga agaagtatgg gaggatgaag tagaagacta
     3361 gaatgagtga aataggcttt aaatacagta ttttagcgtc gggttccagt ggaaattctt
     3421 tttatctgga aacctcaaaa aagaagcttt tagtggatgc aggcttgtct ggcaagaaaa
     3481 ttaccagtct gctagctgaa attaaccgca agccagaaga cctggatgcc atcttgatta
     3541 cccatgagca ttcagatcat atccatggag taggcgtttt ggctcgcaag tatggtatgg
     3601 atctttatgc caatgaaaag acctggcaag ctatggaaaa tagtaaatat cttggcaagg
     3661 tggattcttc gcaaaagcat atttttgaaa tgggtaaaac caaaactttt ggagatatcg
     3721 acatcgagag ttttggtgta agccatgatg cagtcgcacc gcagttctat cgctttatga
     3781 aggatgacaa gagttttgtc cttttgacag atacaggtta tgtcagtgac cgtatggcgg
     3841 gcattgtcga aaatgcggat ggctatctta tcgaggccaa ccatgatgta gagattttgc
     3901 gatcaggttc ttacgcttgg cgactcaaac aacgaatcct atctgacctt ggtcaccttt
     3961 ctaacgagga cggtgctgaa gctatgattc ggacgctagg aaatcgtact aagaagattt
     4021 accttgggca tttatctaag gaaaacaata tcaaggaact ggctcatatg accatggtca
     4081 atcagctggc tcaagctgat ctgggagtcg gagtagactt taaggtttat gatacctcac
     4141 cagataccgc aacaccattg acagagatat agaaagaacg ctgagaaggt gttcttttta
     4201 tggctctttg tcaactgtag tgggttgaaa aaaagctaag cttgagaaag gacaaatttt
     4261 gtcctttctt ttttgatatt cagggcgata aaaatccgtt ttttgaagtt ttcaaa
//
LOCUS       DAVMAF010000111         4287 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMAF010000111 DAVMAF010000000
VERSION     DAVMAF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.112"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            95..928
                     /locus_tag="VYO97_001966"
     CDS             95..928
                     /locus_tag="VYO97_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV1754706.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVLKNFEGQSLINIDRSWFYWDTFLGKLKTYPSLNQVLLKSPVKKELLEMLRDSGL
                     NVMYMPIIKTIEELDFVLSFEGINTVAVELVFQDLENPIISKKVIDDLHEKGLLIWVN
                     ALTLSDSIILSAKIDDDTAIAHDGESWGKLVSIGFDIIQTDWPLLLYQYLVEKNKKIK
                     K"
     gene            954..2015
                     /locus_tag="VYO97_001967"
     CDS             954..2015
                     /locus_tag="VYO97_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1754707.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNELKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            2032..3036
                     /locus_tag="VYO97_001968"
     CDS             2032..3036
                     /locus_tag="VYO97_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754708.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSETQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIEEKVTMYINPRDIMQF
                     "
     gene            3048..>4287
                     /locus_tag="VYO97_001969"
     CDS             3048..>4287
                     /locus_tag="VYO97_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1754709.1"
                     /translation="MRKSLNWKDWLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFHPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFT"
BASE COUNT         1516 a          592 c          778 g         1401 t
ORIGIN      
        1 ttttaaaaaa taatgaaacg ttccaactta tctattgaca cgttccaaga aatcggatac
       61 aatgtaatta acaataaaga taggagaaaa taatttgaat ccattaaaag aatgctatga
      121 aaaaaacggt atactaattg cagcacaccg tggtacaaac ggaggaaata tattacaaaa
      181 cacatcaaaa gcctatttga attccatacg ccataacgca gatatgtttg aggttgatgt
      241 aattcgttca aaagatggag aattttttgc atttcacgat gggcaagaac ctttagtatt
      301 tggtgaaaaa attgatatta gaactttgag ttctgaagag atccaaagaa aggaatgttt
      361 taattcactg aatgaaccca tattacaaaa gattgaaaaa atttctgatg tattaaaaaa
      421 ttttgaggga cagtctttaa taaatataga tagaagttgg ttttactggg ataccttcct
      481 aggtaagtta aaaacatatc catctttgaa tcaagttctt ttaaaatcac ctgtaaaaaa
      541 agaattattg gaaatgctta gagactctgg tttgaatgtt atgtatatgc caattatcaa
      601 aacaattgaa gagttagatt ttgttctttc atttgaagga ataaatacag tagctgtcga
      661 acttgtattc caagatttag aaaatccaat cataagtaag aaagtaattg atgatttaca
      721 tgaaaaagga ttacttattt gggttaatgc tttaacctta agtgattcaa ttattctttc
      781 ggctaagatt gatgatgata cagctattgc tcatgatggc gaatcttggg ggaaattggt
      841 ttcaatagga tttgatatta ttcaaacaga ttggccatta ttactttatc aatatctagt
      901 tgaaaaaaat aaaaaaataa aaaaataaaa aataataata aaggagaact atcatgttca
      961 aaaactttaa aaagattgca ttcacttctt cactaatttt acttgctgct tgctcaaaca
     1021 atgcttcaaa tacaaatcag tctactgatg aaggaagtgt tgataaatct caagaaattg
     1081 taatctatac aaattcggct tctaatggac gtgcagattg gttacaaacc aaagcatcag
     1141 agcaaggata caatcttcgt ttggtagata tttcaggtgg agaactagct gaccgtttga
     1201 ttgcagaaaa gaataatgct gtggctgata tggtaattgg attaaataaa ttagaattta
     1261 accgtattaa agctgaaaaa cttcttgtaa aatactcacc aaaatgggct gatgaagtag
     1321 atagctcttt gggagattct gaagggtatt attcaccaat tgtaaatcaa ccattagttt
     1381 tgattggtaa taaagatgct aaaatgccat ccgactggac agattttact gattcatctt
     1441 ataaaggtaa atatggtatt tcaaaattaa gtacaggtac atctaagaac atctttgcaa
     1501 gtattgtttc gagatataaa gatgaaaaag gtgagttagg aatatctgat gaaggttgga
     1561 aagttgcaaa agcttacctt aaaaatgcac acgtttatgc agaaggtgaa gactatatct
     1621 cttcaattat ggataataat aatgagctaa aatatagtat gatgtgggga tctggtgttc
     1681 tacaaaatca aaaagaacgt gaatataaat tccaagtaat gtcaccaaaa gttggtgttc
     1741 cttatgtaac tgaacaagtt gggattttaa atacaagtaa gaagcaagca ttattaaaag
     1801 aatttgtaga ttggtttggc tcttcagaac ttcaaaaaga atggtctgat aaatttggtt
     1861 caattccagc taataaaaaa gctctagagc aagcaaaaga tgaactcaaa cagtttgcta
     1921 atagtgttaa accacaagaa cttgactggg agtttatcgg aaaaaacatc gattcttgga
     1981 ttgaaaaagc acagcttgaa tttgttgaac agtaatagtt aggattaagt gatgattaca
     2041 tttaaggata ttcaaattaa gtataatgat tttattgcca tcgataatct caatttaaca
     2101 atcaatgaag gagagttttt cacattttta ggaccaagtg gttgtgggaa atcaacaact
     2161 cttcgtgcac tagttggatt tttggagcct tcacaggggc aaatactagt tgataatcaa
     2221 gatattacga atttagatcc ggaaaaacgc gaaattggta ttgtgtttca aagttatgca
     2281 ttattcccaa caatgactgt ttttgataat attgcttttg gtctaaaagt taaaaaagtt
     2341 tctcctgaaa ttattaaagc taaagttcat gaaattgcga aaaaaattaa tatctcagac
     2401 gagcaactaa accgaaatat ttctgaatta tcagggggac aacaacagcg tgtagcttta
     2461 gcacgtgcat tagtattaga accaaaaatt ctttgtttgg atgaaccgtt atcaaaccta
     2521 gatgccaaac tacgagtttc gttgagaaaa gaattgaaac gattgcaaaa agaactagga
     2581 atcactactc tttatgtcac acatgatcaa gaagaagcat taacattatc agataggatt
     2641 gctgtgttta ataatggatt tattgagcaa gttggcacgc cgtatgatat ttataatcaa
     2701 tccgaaactc aatttgtctg tgattttatt ggctcaatca ataaaattag ccaagaagtt
     2761 cttaaaaaat tacaaggcga gaataaatac tttaaagaaa ctgaaggata catacgctta
     2821 gagcgtgtta agttagagaa aacttcacct caagatttat ctataacagg agtaattagt
     2881 gatattgagt ttaatggtgt acttattaag tataaagtag acttgtcgaa tagtgaaagt
     2941 attcatatta tcgaaaagaa tgatggacta tcacgtcata taatcgaaga aaaagtcact
     3001 atgtacatta atccgcgtga tataatgcaa ttttaaggag attgtgcatg agaaagagtc
     3061 taaactggaa agattggctc ataagattag gagttttatg gctaatcttt gccctgatta
     3121 tttatccaaa tattgaactt ttgtctggag tgttttataa aaatggttca tttactctag
     3181 aggcattcaa taagattata cactctcaga gagcgatgag tagtatattt aatagcttta
     3241 ttttggcgat ttcaatggtc tttactgtaa atattgttgg gacattgatt gttctactca
     3301 cagagtactg ggatattagg ggttcaaaaa tacttaaatt aggttatatg agctctctag
     3361 tatatggagg aattgtatta gcgacaggat ataaatttgt ttatggcgct aatggtcttg
     3421 taactaaaac gctctcaagt ataattccag gtcttgcccc taactggttt caaggatttg
     3481 gtgcagtatt gtttattatg acatttgctt gtacctcaaa tcacataatg tttttaacaa
     3541 atgcagttcg tagtgttgat tatcatacaa ttgaagcagc taaaaatatg ggtgcaagtc
     3601 ctttaaaagt tttctttagt gtggtttttc caacttttaa acctacgtta tttgccttaa
     3661 ccatcttaac ttttttgaca ggtctttcag cagtttctgc tcctttgata gtagggggga
     3721 aagattttca aactattaac ccattgatta tcagtttcgc taaaactaca agttcgagag
     3781 atttagcagc tttactagct atcatgttag gtttagcaac tattctatta ttgacacttt
     3841 tgaatcatgt cgaaaaagga ggtaattatg tatctgtttc gaaaaccaag gctagtatta
     3901 agaagcagat aatttcttct ccattatgga atactcttgc tcacattgtt gcgtatgtat
     3961 tgtttgcaat ctatatgtta ccaatagttt tgataattct atactcgttt acagactcgt
     4021 tgacgatcaa gacgggaact ttagatctat ctaaatttac gttagcgaac tatgctaatt
     4081 tatttacaga tgctcaatca tttcatcctt acctagtcag cgttgtttat gcaattttag
     4141 ctgcggtaat tgctacaatt atagcaatag taatttcacg tattgtacat aaaaacaaga
     4201 gtaagttcga taaattcttt gagtatggtg cactaatacc atggatattg ccaggtacat
     4261 tgattgcact aggattaatg ttcactt
//
LOCUS       DAVMAF010000112         4268 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMAF010000112 DAVMAF010000000
VERSION     DAVMAF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.113"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(76..1491)
                     /locus_tag="VYO97_001970"
     CDS             complement(76..1491)
                     /locus_tag="VYO97_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1754710.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1504..3342)
                     /locus_tag="VYO97_001971"
     CDS             complement(1504..3342)
                     /locus_tag="VYO97_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1754711.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3360..4199)
                     /locus_tag="VYO97_001972"
     CDS             complement(3360..4199)
                     /locus_tag="VYO97_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1754712.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKKALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT         1427 a          841 c          559 g         1441 t
ORIGIN      
        1 cttccaaacc tctttatttc atatttaatg agatgttgcc ttaaccgtga taatatctaa
       61 tcatcaataa acaaactatt tgaataagga ttctccattt gattcaatca ctcctttata
      121 ccaagtaaag gacattttct tatatcgatt gaatgtgcca tttccatcat catttcgatc
      181 aacataaatg agaccgtacc ttttagaaag ttgtgcagtg gacatagaaa cacagtcaat
      241 acatccccaa gacgtatagc ccataatttc aacaccatcc tgtagagctt cagcaacttg
      301 caataaatgt tctttcatat actgaattct ataatcatct tggacggtta agttattaag
      361 ttcatctttt attagttgat ctttagcacc taatccattt tctactataa ataatgggat
      421 ttgataacgg tcataatatc tatttaaaat tatacgtagt ccaattggat caatttgcca
      481 tccccactct gaagactcta aataaggatt tactaaacca ccaataatat tcccttctcc
      541 tgaattatac tgtgttggaa tagcagattg agtcacactc atgtaatagc taaaggataa
      601 aaaatctaca gtataatttt ttaataactc tgcatcttca gctgcaaact ctatgttaat
      661 gtcattttcc ttaaaatatc tttttgcata attcggataa taacctctaa catgcacatc
      721 tgaaaataga taatttagat tctcatactc atgagtcgcc catacatctt ttggatttgg
      781 agtcattgga taagctggca tagctaatac catacatccc accttaaact ctgaattaat
      841 ctcacgagca atttttgtaa ccaaacttga ggcgactaat tcatgatgta tagcttgata
      901 taattcttgt ttcgaaagat tctccttagg tatatctatt cctccactag taaatggtaa
      961 ttccaaaaca gagtttactt cgttaaatgt aagccaatat ttaactttat ctttatacct
     1021 ttctaaaact gttcgagcaa atttttcata aaaatgaatc attctcctat caacccatcc
     1081 atgatatttt cttgctaaat ataatggagt ctcatagtgt gaaagagtta caagtggctc
     1141 tatcccgtga gcatgtagtt catcaaacaa ttcatcataa tatttcaaac cagcttcgtt
     1201 aggttcttct tcatctcctt ttggaaaaat tctactccat gcaatagaag tacgaaaaac
     1261 attaaagccc atttcagaaa acaaggatat atcttcctta tatttatgat aaaaatcaat
     1321 acctatcaat tttaagttat cttctgtagg attttctgtt gcttctccta atccaccttt
     1381 gggtaacaca tcctgaactg ataagccctt accatcttca ttatatgctc cctctacttg
     1441 attagctgca acagctccac cccaaagaaa atcatctgga aaaatggtca taactttcct
     1501 ccattataat attaccagta attccttaga atgctcgatt gtctgattat taggtaatac
     1561 taatacatct agaaaatcat tggtattcgt tacaattact ggtgtaactg tttcgtagcc
     1621 tttagtcttg attaaattca agtccatttc aaaaatcaac tgatttttga aaactctgtc
     1681 tccttcttct acatgactaa taaaaccttg accttttagc tcaacagtat ctaatccaat
     1741 atgaattagt aactcaacac cctcatcact cttcaatcca attgcgtgct tagtcggaaa
     1801 aatatttgta attttcccat caaatggtgc ataaacctta ccttcacttg ggataatcgc
     1861 tactccgtct ccaattagtt tatctgaaaa tgttttatcc tggacatcgc ttaacggaat
     1921 gatttctcct gatataggag aaaatatcat ttttttattt gaaactccag ctttaacttc
     1981 taaattgcta gaactctctt cttcatcgat tccaaatata taagctaata caaaggtaat
     2041 aacaaccgaa atgaccgcca caattaaagc atttacaata tttgatggca catcagaata
     2101 aataaattga ggcaacgcta tcaaagatgg gacagcaaat agatatgctt taacactagt
     2161 aagacctgca aataatcccg ctaatccacc accaatcata gctgcataaa gcggtttttt
     2221 atattttaaa gtcacaccat ataatgcagg ttcggtaatc cctgcaagta aggctgagaa
     2281 acctgctgca aaagcaattt gttttgtatt attattttta ctctttaatg caacagccat
     2341 cgaagcagcc ccttgagcta agtttgaccc taacattgct ggaagaatta atacgtctgg
     2401 agtagcaata gatgccgcca aaaaaatagg tgcaaaagcc caatgcattc cagtcataac
     2461 aataaatggc ataatagcac caagaatagc taatgtaagc catccagcta caccatacat
     2521 ttgcccaact agatttgata atccttcacc aacaattact ccaataggtc cgactacaac
     2581 taaggcaata cagcttgata ctaataatac tagcgtaggt tgcaaaaaac tcttagtaac
     2641 agctggtgtt aatttagcaa tcattttttc aatatatttc atcaaccaaa ccataataag
     2701 aattggaacg actgacgaac cataactagc tggtgtcaca ggtgcaccaa ataaactaag
     2761 aggattccct gattgcacca tttgaacaaa atttggatgg agaagtacac ctgctacaga
     2821 catagctaat gtagatgtta cttttaattt ttgtgatgca gaataagcta ataacagcgg
     2881 taagaaataa tatggagcat ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga
     2941 ttgcaatata ccaagcattg gtaaaataat taccaagact ttcagcatac ctccccctaa
     3001 cattgctgga atgattggag tcatggaacc agcgatatac tcaatgattc tttctaaaat
     3061 attccctttg tgcccttgaa caactgaatc ggattcaaaa ttgccaagtt taacgaattc
     3121 tttataataa ttagctacat cattaccaag tataatttga tattgtccat tctttttcat
     3181 aatacctatt acacctggta tcttcttcac atcatcatca ttgactaaat tttcatcttt
     3241 taattctaat cttaaacgtg ttacacaatg ggtaactcta ttgacatttt tttcacctcc
     3301 aattacatcg aggatttttt gtaccgtatc tttataactc atggtattct cctattctat
     3361 taatctaaat tttttgttaa gcgacgaata tgagccatca aataaactaa ttcactagaa
     3421 gtcagcaaat aattgtactc agtttgtata aacattgcca cctgttcacc acattcatat
     3481 tctctaggat atttattttt cattaatgct aacaagtctt catcatcatc gtcacaataa
     3541 gttgtatttt caaccaagcg atgggcaaat aattttatat gagttatgaa tctataataa
     3601 tcaatagaat cttggttaaa ctcagtacaa tagaaatctt gaatgatttt ctcaattccc
     3661 ataattattt cagtgatttt atacgactct tgaaaattat tttctaaatt agcattaaca
     3721 aaatgtagcg ctatgaatgc agcttcgtca attggaagtt caatacctaa ctcttttttt
     3781 attatttgta aagccttttt cccaagctca aattcttctt tataatattt cgagatttcc
     3841 cagcgtaggg gattcgaaat aataactcct tctttataac gttctatact cgaagaaata
     3901 tgatccgtta gatttatata gataatctca tcaaaattat tacccaatgt tattttacct
     3961 aggttaatta ttttttcggt gatgtacacc aactccaaag gaacgttaat aaacatctct
     4021 gtaaaacggg tcatattatc tctatttttg agaaaaaaac ttttctcaat atcaattgga
     4081 tttactttat ctccaatttt tcttccaaaa gctattcccc ttccaaaaag aagaatatcg
     4141 atatcttttt tactttgcgc aattacggca ttgtggttta gtattcgttt tatgatcata
     4201 aaatttccct tttaaaataa aaggaaccta cttaaaaaac atcaagaaac ttcttctgat
     4261 gtgattaa
//
LOCUS       DAVMAF010000113         4244 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMAF010000113 DAVMAF010000000
VERSION     DAVMAF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.114"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            52..2385
                     /locus_tag="VYO97_001973"
     CDS             52..2385
                     /locus_tag="VYO97_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1754713.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVGEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2398..3861
                     /locus_tag="VYO97_001974"
     CDS             2398..3861
                     /locus_tag="VYO97_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1754714.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            3861..>4244
                     /locus_tag="VYO97_001975"
     CDS             3861..>4244
                     /locus_tag="VYO97_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1754715.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFVDGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTE"
BASE COUNT         1481 a          636 c          890 g         1237 t
ORIGIN      
        1 tgatagttta ttttatggag aagtagattt ttagaatgcg gagggttcaa tatggttgag
       61 tttataaagt ctaagaaaga aatgagtgag gaggatatta aagcaaattt catcactcct
      121 gctattgtat ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga atacttcact
      181 gatggtcgaa ttgaggttag aggagataag gctcgtcgta aagaaggaaa aaaatcagac
      241 tattcactgt attaccaatt tggaactcga attgcaattg ttgaggcaaa ggataataaa
      301 cacagcgttc gagcaggatt acaacaagct attgaatatg gagagatttt agatgttcca
      361 tttgtttatt cttcgaatgg tgatggcttt attgaacacg accgtatcac gagagaagaa
      421 cgtgagctgg agttagacga attccctact cgtgaagaat tattttctcg tatgacgaag
      481 gaaaaaggat tgacgtacga aattacagaa gctatctcaa ctccatacta tacagacgcc
      541 ttctcaatga aaacgccacg ctattatcag caaatagcta tcaaccgtac tattgaaaca
      601 gttgccagag gacaaaaacg agtaatgttt gtgatggcaa caggaacggg gaaaacgttc
      661 atggcttttc aaattattca tcgccttcga aaagctggtt tggctaaacg agttttattc
      721 ttagcggata gaaacatctt agtagaccaa acgatggctg aagactttag gccattcgaa
      781 aaggtaatga cgaaaattac accaaaactt ttgactgctc ctgaaaaatt aaattctttt
      841 gaaatttatc tagggcttta tcagcaacta actggtgaag atggaactga aacacattat
      901 caaaaatttg acaaagactt ctttgattta atcgtaattg atgaagcgca ccgtggttca
      961 gctaaggaaa acagtaactg gcgtaagata attgattatt tcagttctgc gacacagatt
     1021 gggatgaccg ctactcctaa agaaaccaag aatgcttcca atacggaata ctttggtgag
     1081 ccaatctata cttatagttt aaaacaggga atcgaggatg gttttttggc tccatatcgt
     1141 gttatgagag ttaatttaga tgtagatgtg gatggttatc gtccagaaac tggaaaagtt
     1201 gatgctaacg gacaattaat agaagatagg tactacggca ggaaagattt tgataaaacc
     1261 attgtcattg atgatagaac gcaaagagtt gccaagtttg tttctgatta tatgaagcaa
     1321 aacaatgcac gatttgataa aacaattgtt ttttgtgttg atattgacca tgccgagcga
     1381 atgcgtgctg cacttgtaaa agagaatcta gacttagtcc aagaagacta tcgttatgtc
     1441 atgcaagtaa ctggtgacaa cgctgaagga aaagctcaac tggataactt tatggatgtc
     1501 aattctaatt ttcccgctat tgtaacaacg tctaaattat taacgacagg agttaatgct
     1561 aaaacatgtc gtttgattgt tttagactct aatatccaat ccatgactga atttaaacaa
     1621 attattggtc gtggcacacg tctttatcct caaaagggga aagaattttt tacgattatt
     1681 gattttcgaa atgttaccaa tttgtttgct gaccctgatt ttgatggtga tccagtgaag
     1741 gtgctggaaa caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt aggtgaggaa
     1801 ggtgacccag tagaaaaata tatcgttaca gacaagcagg ttaccattct taattctact
     1861 gttcaagtat tggatgaaaa cgggaaactg attaccgaaa gcctgaccga ctacactcga
     1921 aagaatatct taggtagcta cgccactttg aacgatttta tcacagtttg gcatacggca
     1981 gataaaaaga agcttatctt agacgaactt tataaaaaag gagtttatct agatgctatt
     2041 cgagagtcgg agggaatatc agaacaagaa atcgatgatt ttgatttact cctaaaactt
     2101 gcctatggtc aaaaagaatt aaccaaaacg gaacgtatca ataaactcaa acaaagcgga
     2161 tatttatata aatatagtga ggaagcgcgt gctgttttgg aaattttact gaacaaatac
     2221 atggataaag gtattggaga actcgaaagc attgaaacat taaaacttcc agaatttcag
     2281 atatatggtg gaaccttcaa aatcatcaat acttattttg gagataaaaa acgatattta
     2341 caagcaatta aagaattgga gcaagagcta tttacagtag cttaatgaaa ggaaagtatg
     2401 tcaattacat catttgtaaa aagaattcaa gatatcactc gaaacgatgc tggtgttaat
     2461 ggtgatgctc aacgtattga gcaaatgtct tggttattat tcttaaaaat ttatgatagc
     2521 cgtgaaatgg tttgggaatt agaagaagac gagtatgagt caattatccc agaggaatta
     2581 aaatggcgaa attgggctca tgctcaaaat ggggaacggg tattgacagg cgatgaatta
     2641 cttgattttg tcaataacaa gttattcaaa gagttgaaag agcttgaaat aacttcaaat
     2701 atgcctattc gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa ctatatgaaa
     2761 aatggcgtct tgttacgcca agtcatcaat gttattgatg aagttgattt caatagccct
     2821 gaagatcgtc attcgtttaa tgatatttac gaaaaaattc ttaaagatat tcaaaatgct
     2881 gggaactcag gagaatttta tacgccacgt gcagcgactg attttattgc cgaagttctt
     2941 gacccaaaac ttggagaatc aatggcagac cttgcttgcg gaacaggagg cttcttgact
     3001 tcgactctga accgtttaag tagtcaacgt aaaactagtg aagataccaa aaaatataat
     3061 acagctgttt ttggtattga aaagaaagca tttcctcatc ttttagcagt tacaaatctg
     3121 tttcttcacg aaattgatga ccctaaaatt gttcatggaa atactttgga gaaaaatgtt
     3181 cgtgaatata cggatgatga aaaatttgac attattatga tgaatccacc ttttggaggg
     3241 tcagaattag aaacaataaa aaataacttt ccagcagaat tacggagttc tgaaacagct
     3301 gatttattta tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt tggagttatt
     3361 ttacctgatg gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca aaaactggta
     3421 gatgagttca acttacatac gattattagg ttgcctcata gtgtctttgc accgtataca
     3481 ggaatccata cgaacattct tttctttgat aaaacaaaga aaacagaaga aacttggttt
     3541 tatcgtttag atatgccaga tggttataaa aatttctcga aaactaagcc gatgaagtca
     3601 gaacacttca atcctgttcg tgactggtgg gaaaatcgtg aagagattct ggaaggtaag
     3661 ttctacaaat ctaaatcatt tacacctagt gaattggctg agttgaatta taatttagac
     3721 cagtgtggct ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt gattcagaat
     3781 tatcaagcgg aaagagcaac tttaaatcat aagattgata atgtattagc tgatattttg
     3841 cagttgttgg aggacaaata atgacaccag aacaacttaa agcaagtatt ctccaaagag
     3901 cgatggaagg gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt gaattattaa
     3961 agagaattaa agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa cgagataaaa
     4021 aggaaactga gatatttcgt ggtgatgatg ggaaacatta tgggaagttt gttgatggaa
     4081 gtactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggtttt
     4141 ctacattggt tgaaattgtc agaggtggct ctccacgacc aatcaaagat tatcttactt
     4201 ctgaagtaga tggaataaat tggataaaaa taggtgatac tgaa
//
LOCUS       DAVMAF010000114         4116 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMAF010000114 DAVMAF010000000
VERSION     DAVMAF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.115"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            605..1597
                     /locus_tag="VYO97_001976"
     CDS             605..1597
                     /locus_tag="VYO97_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754716.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1599..2417
                     /locus_tag="VYO97_001977"
     CDS             1599..2417
                     /locus_tag="VYO97_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1754717.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2419..3204
                     /locus_tag="VYO97_001978"
     CDS             2419..3204
                     /locus_tag="VYO97_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754718.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3302..3520
                     /locus_tag="VYO97_001979"
     CDS             3302..3520
                     /locus_tag="VYO97_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1754719.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3505..3702
                     /locus_tag="VYO97_001980"
     CDS             3505..3702
                     /locus_tag="VYO97_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1754720.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(3978..>4116)
                     /locus_tag="VYO97_001981"
     CDS             complement(3978..>4116)
                     /locus_tag="VYO97_001981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754721.1"
                     /translation="SKHCFEVVDRTDEVSNHTHGKATLTWFEEIFEEYKSKTFVNRDT
                     L"
BASE COUNT         1131 a          716 c          894 g         1375 t
ORIGIN      
        1 gatgaaagcc ttaagtgtta ttttataaag gttatttcaa gtcgttccaa ggtaacaagt
       61 ctagatcaga ttgaagctga taaaacgata caaagaaaat attcaagtga gctaaaaaaa
      121 tttattggat tttataatga gattatttgt gaggaaaata gtttcctaca tgtacgaaag
      181 aggtggtcga gttggtttag gtagtcgatg cgtgagttga taattctcag ggtatggact
      241 tctttttcat gaatgaggta aaagagcagg tattgtttag agacaatcat tctgagcata
      301 ttttctggat agaggaagta tccgatttta tgatcaaagt taataccgcc ctctggtgag
      361 aagatgagta ggttggtaat ttaaactatt aaacagaatt tttgattaaa agtattattt
      421 tatgagagaa atcctaattt cacaatccat aggcaaactc ttgcatttcg ttttttattg
      481 gactataata ggttggtata aagccttttg tagtaataaa atgtagaagg tgtagaaagc
      541 aaggatttag aatatttgta gttaaaaaca caatgttgct attccttacg atagggagat
      601 agatatggca atgatagaag tggaacatct tcagaaaaat tttgtgaaga ctgttaagga
      661 accgggcttg aagggggctt tgcgctcctt tattcatcct gaaaagcaga cctttgaagc
      721 ggtcaaggat ttgacctttg aggttccaaa agggcagatt ttaggattta tcggggcaaa
      781 tggtgctggg aagtcgacaa ccattaaaat gctgacagga attttgaaac caacatctgg
      841 tttttgtcgg attaacggca agattcccca ggacaatcgg caagattatg tcaaagatat
      901 tggcgtagtc tttggacaac gcacccagct atggtgggat ttggctctgc aagagaccta
      961 cactgtctta aaagagattt atgatgtgcc agactcgctc tttcataagc gtatggactt
     1021 tttgaatgaa gtcttggatt tgaaggactt tatcaaggat cccgtgcgga ctctttcact
     1081 gggacaacgg atgcgggcgg atattgcggc ctccttgctc cacaatccca aggttctttt
     1141 tttagatgag ccgaccattg gtttggacgt ttcggttaag gataatattc gtcgggcaat
     1201 tactcagatc aatcaagagg aagaaactac cattcttttg accactcacg atttgagtga
     1261 tattgagcaa ctttgtgatc ggattttcat gattgacaag gggcaagaga tttttgatgg
     1321 aacggtgagc caactcaagg agacctttgg taagatgaag actctctctt ttgaactgct
     1381 accaggtcaa agtcatctcg tctctcacta tgaaggtctg tctgatatga ccattgatag
     1441 acaaggaaac agcctcaaca ttgaatttga tagttctcgc taccagtcag ctgacattat
     1501 caagcaaacc ctgtctgatt ttgaaatccg cgatttgaag atggtggata cggatattga
     1561 ggatattatc cgtcgcttct accgaaagga gctctaggat gatcaaattg tggagacgtt
     1621 ataaaccctt tatcaatgca ggggttcagg agttgattac ttaccgagtc aactttattc
     1681 tctatcggat tggcgatgtc atgggggctt ttgtggcctt ttatctctgg aaggctgtct
     1741 ttgattcttc gcaagagtct ttgattcagg gcttcagtat ggcggatatc accctctaca
     1801 tcatcatgag ttttgtgacc aatcttctga ctagatccga ttcgtccttt atgattgggg
     1861 aggaggtcaa ggatggctcc attatcatgc gtttgttgcg accagtgcat tttgcggcct
     1921 cctatctttt caccgagctt ggttccaagt ggttgatttt tatcagcgtt ggccttccat
     1981 ttttaagtgt cattgtcttg atgaaaatca tatcgggtca aggtattgta gaggtgctag
     2041 gattaactgt cctttatctt tttagcttaa cgctcgccta tctgattaac tttttcttta
     2101 atatttgctt tggattttca gcctttgtgt ttaaaaatct ttggggttcc aacctactta
     2161 agacttccat agtggctttt atgtcgggga gtttgattcc cttggcattc tttccaaagg
     2221 ttgtttcaga tattctttcc tttttgcctt tttcatcctt gatttatact ccagttatga
     2281 tcattgttgg aaaatacgat gccagtcaga ttcttcaggc actccttttg cagttcttct
     2341 ggctcttagt gatggtggga ttgtctcagt tgatttggaa acgggtccag tcctttatca
     2401 ccattcaagg aggttagtat gaaaaaatat caacgaatgc atctgatttt tatcagacaa
     2461 tacatcaaac aaatcatgga atataaggtg gattttgtgg ttggtgtctt gggagtcttt
     2521 ctggctcaag gcttgaatct cttgtttctc aatgtcatct ttcaacatat tccattccta
     2581 gaaggttgga cctttcaaga gatagctttc atttatggat tttccttgat tcccaaggga
     2641 atggaccatc tcttttttga caatctctgg gcactagggc aacgcctagt ccgaaaagga
     2701 gagtttgaca agtatctgac tcgtcccatc aatcctctct ttcacatcct agttgaaacc
     2761 tttcagattg atgccttggg tgaactctta gtcggtggta ttttattggg aacaacagtg
     2821 accagcattg tttggactct tccaaaattt ctgcttttcc tagtttgtat tccttttgcg
     2881 accttgattt atacttctct taaaatcgca acagccagta tcgccttttg gactaagcag
     2941 tcaggcgcca tgatttacat cttctatatg ttcaatgact ttgctaagta tccgatttct
     3001 atttacaatt ctcttcttcg ttggttgatt agctttatcg tgcctttcgc ctttacagcc
     3061 tactatccag ctagctattt cttacaggaa aaggatgtgt tctttaacgt aggaggtttg
     3121 atgttgattt ctctggtttt ctttgttatt tcccttaaac tttgggataa gggcttagat
     3181 tcctacgaaa gtgcgggttc gtaaaagcta aagtaagact aaaatcaaga aaggaactta
     3241 tgatgtttgt aattgaagaa gtcaaggatg aaaatcaaaa aaggcagttg tcgctgagat
     3301 tttgaaggat ttgccagaat ggtttggaat cccagaaagc acacaaacct atatagaagg
     3361 aaccacgaca ctgcaagttt ggaccgccta tcaggagagt gatttgactg gatttgtaag
     3421 cttatcctat tcgagtgaag attgtgcaga gattgattgt ctcggcgtaa aaaagcttat
     3481 caaggtagaa aaattgggag ccaattgctt gctactttag agagtgaagc tcgtaaaaaa
     3541 gttggttatc tgcaggtcaa aacagtggca gaagcttcta ataaagatta tgatcgaaca
     3601 aatgactttt atcgaggtct tggctttaaa aagttagaga tttttcctca actatggaat
     3661 ccgcaaaatc cttgtcagat tttgattaaa aagcttgaat aatattactt gacatctatt
     3721 ctcagagtgc tatactgtaa gtgtaatcgc cgatttagct tagttggtag agcaaggcac
     3781 tcgtaaagcc taggttatag gtagataaac gactgaggat ttgaaaaaat agataggtag
     3841 aagataaccg ttaagcctta ctcttagcgg ttatttatat tgtttaatag cgctaatatt
     3901 ttatcaatta tgcctgtttt cgtgtttctg gtagttgttc aagtttattg ctactatttt
     3961 tgatggtatg aatgtgctta taatgtatcc cggttaacga aagttttgga cttatactct
     4021 tcgaaaatct cttcaaacca cgtcaacgtc gccttgccgt gcgtatggtt actgacttcg
     4081 tcagttctat ccacaacctc aaaacagtgt tttgag
//
LOCUS       DAVMAF010000115         4070 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMAF010000115 DAVMAF010000000
VERSION     DAVMAF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.116"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..199
                     /locus_tag="VYO97_001982"
     CDS             <1..199
                     /locus_tag="VYO97_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1754722.1"
                     /translation="NELLIVGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANR
                     WNRGLEAYKDKVYTRGIWIRD"
     gene            594..1457
                     /locus_tag="VYO97_001983"
     CDS             594..1457
                     /locus_tag="VYO97_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1754723.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDPIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKLLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFK
                     LRLQQYKALID"
     gene            1743..1943
                     /locus_tag="VYO97_001984"
     CDS             1743..1943
                     /locus_tag="VYO97_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1754724.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2010..2675
                     /locus_tag="VYO97_001985"
     CDS             2010..2675
                     /locus_tag="VYO97_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1754725.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2747..3973
                     /locus_tag="VYO97_001986"
     CDS             2747..3973
                     /locus_tag="VYO97_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1754726.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTIISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         1178 a          718 c          785 g         1389 t
ORIGIN      
        1 taatgaacta ttgattgtcg gtataaaaat tttagtatat gaaccaaatt taaacgttag
       61 tatagataac gtaatttttg aaaagaattt tgaactattt acaaaacaaa gtgatctaat
      121 tgttgcaaat agatggaata ggggccttga agcttataaa gataaagttt acacaagggg
      181 aatctggata agagattaac tattgatatt aaaaaggcaa taatttataa aatttgtaca
      241 aaaaaatgtt aaattttcac attttcatag tatactgttt aatagaaagt aggttctttg
      301 taaccgctcc attataaaat cctttcactc ttccggtatg ctattaggaa aaacataatt
      361 ggaggatatt ttgagctcac acctcatcgt tccagtggct gttccccaag ctcgtgtact
      421 gcccaaaaaa gttagacatt ctatttagaa cgaggattga gttctgtatt gtacaaggct
      481 cggtcctttt agagtcagct taaggctggc tttttcaatc accaaagtgt cagaatgttt
      541 tgacaaatga acacaaataa tgatataata tgcaaaagct aggaggtggt aggatgattg
      601 aaaaaatgga actgggggaa ttttacaagg aattacgttt ggcgagaaag cttaagcaga
      661 cagatgtggc ttgtgagggt ctaacagctt ctcagttgtc caagtttgaa ctagggcagt
      721 ctatgctgtc tgcggataag ctcatattag ccatccaagg gatcaatgtg acctttgatg
      781 agtttgggca caagctcaac aactatcaag aatctccaca tatgcagatt ggtagaaagg
      841 ttgtggatcg ctttgctcat caagatatag ctggcttaga gcaactgttg gaggaagtcg
      901 agcaagaaca gatggcagag acttatcgtc gtttgaatgc tattgtaatc aaagatgcca
      961 tccactcact agataaaagc tacccactag cagaggagga tagcgagttt ttgaccacct
     1021 atctctatgc tatcgagtct tggacctggt ttgaactcta tctcttttgc aatactatgc
     1081 ccttcttgag caatcaagat ccgatctttt tatcaacctc cttactcgaa aaatccaaag
     1141 aatttaaaga gttagtacac aatcgattgt atatgaagca aggactctta aatatcatct
     1201 cagagctcat ggagcgcaaa cttttttctt atatcccaat ctttgaagcc gagctggaga
     1261 gtatgctccg tccatacgat gtttttgaga aattattgtg gcagttttta aagaagatga
     1321 gcattttcct tcaaactaag ggaagcaatc aaaaagagat tgaacacttt atccaatctc
     1381 tgcaagtatt agaaaatcca caattaacag ccctttttaa actacgctta cagcaataca
     1441 aagcacttat cgattagtta gaaatgggaa aaagataata atctcagcaa cgattgtcaa
     1501 taatgttaaa acttgtttct tcatatgact tccctcctca ttagtaataa ctttattata
     1561 ccagggaaat aatcaaatat accaaaatcg caaatctgaa atttctataa gaaaaaatat
     1621 caaatatgcg attttctaaa ataagccaat tttcgtgtta tactgtactt gtaaagcact
     1681 tgaagcaaat cctaggtcgc agaagtggtt tacaaatgaa gataattgaa ggagtgtaaa
     1741 agatgttaaa tttacaattt gcagaaacaa tggaattgac agaagctgag ttggagacag
     1801 tttatggagg ggaatttggg aataatgctg ttatcccagc cggtgcttgg ggagggttcg
     1861 ggacaccttg gtcaatcact aatttctgga agaaaaattt taacgataga cctgactttg
     1921 attctgaccg ccgtcgttat taatatccaa agcccttgcc ttttcttggg taggggctgt
     1981 ctttatttca tagtatagga ggaaatagaa tgaaaaagat aatctcacac cgctacttta
     2041 ttatagtttt tctactagtt attgctgacc agaagttcag tgttctagtt ttacgcagcg
     2101 accttgttac tgggctatct gactttgcct attatctgtc ggatatgatg ttgaattttc
     2161 ttgtggtttt atttgctctt attgctatga tttggtcggg gaaatggcag aaaatcaata
     2221 gtagaaagtt taaaggatcc tatcttttct attccttcct agctctgctt gcttttgttg
     2281 tttggaattt cgttaccttt tttcttttcc cacctactcg aaatgaaatt tcttatcaac
     2341 atgctgctcc gacttttaca ggggctacag catttttgat gtattttttc tatcctgtga
     2401 ttgcaggtcc catttttgaa gagataatcc atcggggatt ggtgatgaca gctctggaaa
     2461 aaggaaagaa atgggggcta gatgtacttg gttccgctgt tttatttggg gtctcgcaca
     2521 ttagtaatca cggttgggtc ttgacagact ttgtcttcta tatgggtgga ggtcttatat
     2581 ttgcagtctt atttagaatg acaaagtcaa tttattggcc tattggactg catatagtct
     2641 acaatggcat tggtcagctt ttgatgttac tgtaattttg gttgttagta gtatcgcggt
     2701 ctatccttgg ggaaagattc ccatttgaac ttaagaagga gttgtgatga aagtatttct
     2761 tcaaaataga gattttaggc aattaactat caaccagtgg atttcaacgc ttggggatac
     2821 gattttttat ctggcctttt tgaattatgt agcagatgcg tcttttgccc ctttggcgat
     2881 tttacttatc acgatttcag aaacccttcc ccaagttcta caaatctttc tgggagtttt
     2941 ggcagatttt caacatcatc gtgtcctaaa atatacaatt attagttttg caaaattttt
     3001 gctttactct atagtgtccc tatcgctttc agggcagtct ttctccttgt tgctagtagc
     3061 atttatttgt ctgattaacc tcttgtctga cacattgagt tacttttcag gagccatgct
     3121 cactccgatt ttcattagaa ttattgggca agaccatctg gcagaagcta ttggatttaa
     3181 acagtcaact gttagtttag tgaaaacaat cagtaatatt ctaggaggag tcttgctagg
     3241 gattctctcc atccagttca tttccttgct gaacgctctt acctttttaa tcgcattttt
     3301 aggtatcctc ttcataaaaa ctgacctctt gaaagtagaa aaaacgatta actatcaaga
     3361 aggactctct gtaaaatcct tttgccagca tttgctccaa tcatcgaaat tgatatggaa
     3421 tatgaataag gtgctcttgg ttttgtttat tatctctact agtcaagcag tgataaatgt
     3481 tacagttcct gtctctactc tatttttgag gaatcaaccc tttttgaatt tacaaacagg
     3541 tcaatctctt gccttgctat ccacttttga actgtcagcc ctgattgtcg gaagccttgt
     3601 aagtggctat ctgcaacata ccatttccat aaaaacagct ctctatgcct cacttgtcat
     3661 ccagttgctt cttctagtag gatttgccac agttcgtttt gactggattc tcatctttag
     3721 taccttggat gcctttttcg caggtgttct ctctcctaga ttgcaagaac tcgtctttaa
     3781 acaaatacct gaggagtcaa tgggagcagt tcaatcctct atcggtgcca ttacggttgt
     3841 tttacctagc ttatttacaa tagctttggt aaccattgct actagctttg gaactctggc
     3901 agttagcttt gttttattgc tatttcttct agttgccttt gttatgcttc tgaatattcg
     3961 tgaaagtatt tagtggagaa tttctataat atatgtttga gactggattg ccagtctttt
     4021 tatgatggtc aaaatcaaca gacttttttc tcttttctga atgtgttata
//
LOCUS       DAVMAF010000116         4014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMAF010000116 DAVMAF010000000
VERSION     DAVMAF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.117"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(161..1831)
                     /locus_tag="VYO97_001987"
     CDS             complement(161..1831)
                     /locus_tag="VYO97_001987"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1754727.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKLR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2061..2750
                     /gene="coaB"
                     /locus_tag="VYO97_001988"
     CDS             2061..2750
                     /gene="coaB"
                     /locus_tag="VYO97_001988"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1754728.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPESHPNLSIREITNTKDLLIEMQERVQDYQILIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNSTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2762..3313
                     /gene="coaC"
                     /locus_tag="VYO97_001989"
     CDS             2762..3313
                     /gene="coaC"
                     /locus_tag="VYO97_001989"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1754729.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLSLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3297..3860
                     /locus_tag="VYO97_001990"
     CDS             3297..3860
                     /locus_tag="VYO97_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1754730.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1213 a          891 c          724 g         1186 t
ORIGIN      
        1 aactgacaga gtcagtatca tatacctacg gcaaggtgaa gctgacgtag tttgaaaaga
       61 ttttcgaaga gtataaatct tattgatgaa ctgcttgcag tctgagaaaa aatgagcttg
      121 gatattattt ccaaactcac ttaaagtcaa tttcaatcca ctagaacaag cctagtacag
      181 ttccatcgct ttcaacatcc atgttgagag ctgctggacg ttttggaaga cctggcatgg
      241 tcataacatc accagttaag gcaacgatga agcctgcacc taattttggt accaattcac
      301 gaatggtaat ttcaaagttt tctggtgctc caagcgcatt tggattgtct gagaaactgt
      361 attgagtttt agccatacaa attggcaatt tgtcccaacc gttttgaacg atttgagcaa
      421 tttgtgtttg agctttcttc tcaaagttca ctttgctacc acgatagatt tcagtgacaa
      481 ttttttcaat cttttcttgg acagaaaggt cattatcgta caaacgttta tagttagctg
      541 gattttcagc aattgtctta acaactgttt cggcaagtgc tactccacct tctgctccat
      601 cagcccagac actagccaat tcaactggta catcgattga ggcacagagt tcttttaagg
      661 ctgcaatttc agcttctgta tcagatacaa attcgttaat agctacaact gctggaatac
      721 cgaacttacg gatattttca acgtggcgtt tcaagttagc aaaacctgca cgaactgcct
      781 ctacattttc ttcagtcaga gcgtctttag ccacaccacc attcatctta agggcacgaa
      841 gggttgcgac aataacaact gcatctggag atgttggcaa gtttggtgtc ttgatatcaa
      901 ggaatttctc agcaccaagg tccgcaccaa aaccagcttc agtaacagtg taatcagcca
      961 agtgaagggc tgttgtcgtc gccaaaacag agttacagcc atgagcgata ttggcaaatg
     1021 gaccaccgtg tacaaaggca ggtgtaccgt aaattgtctg aaccaagttt ggcttaatag
     1081 catccttcaa aatcaaagcc aaggcaccct caacctgcaa atcacctaca gaaacaggcg
     1141 tacggtcata gcgataacca ataacgatat tcgccaaacg aagtttcaag tcctcgatgt
     1201 ccgttgccaa gcaaagaatt gccatgattt ctgaagcaac tgtaatatca aaaccgtcct
     1261 cacgtggaat accgtttaga ggaccaccaa gaccaacagt cacatggcgg agcgcacggt
     1321 cgttcaagtc cacaacgcgt ttccagagga tacgacgttg atcaattccc agctcattcc
     1381 cttggtgcaa gtggttgtca atcaaggcag aaagggcatt gttggcagtt gtaatagcat
     1441 gcatatctcc agtaaagtgg aggttgatgt cttccattgg cagaacttgt gcatacccac
     1501 caccagcagc accacccttg atccccatga ctggaccaag agacggttcg cggatagcaa
     1561 tcatggtttt cttgccaatc ttgttcaagg catccgcaag accaatggta agcgtcgact
     1621 ttccttcacc tgcaggtgtt gggttgatgg cagtaaccaa gatcaattta ccgactggat
     1681 tgctctcaac tgcccgaatt ttatcaaagc tgagtttagc cttgtacttt ccgtacaact
     1741 ccaaatcgtc gtaagaaata ccaagtttct ctacaacatc aacaattggc ttcaactcaa
     1801 tactctgtgc gatttcaata tctgttttca ttcaaaattc ctctaacctc ttatatgata
     1861 attcattata tcacaaaaca agatttttaa catcctaaaa ctctctaaac gttcgtaaat
     1921 atctctgttt ttaagacttt tagagtcctt tcttaaattt tatatggctt tatagtttga
     1981 aactataata aatcttcgtt tttaccaaaa atttatcact ttcattttac ttaccgctta
     2041 tttttgtgta caatagtgct atgaaaattt tagttacatc gggcggtacc agtgaagcta
     2101 tcgatagcgt ccgctctatc actaaccatt ctacaggtca cttggggaaa attatcacag
     2161 agactttgct ttctgcaggg catgaagttt gtttaattac gacaaaacga gctctgaagc
     2221 cagagtctca tcctaaccta agtattcgag aaattaccaa taccaaggac cttctaatag
     2281 aaatgcaaga acgtgttcag gattatcaga tcttgatcca ctcaatggct gtttctgact
     2341 acactcctgt ttatatgaca gggcttgagg aagttcaggc tagctccaat ctaaaagaat
     2401 ttttaagcaa gcagaatcat caggccaaga tttcttcaac tgatgaggtt caggttttgt
     2461 tccttaaaaa gacacccaaa atcatatccc tagtcaagga atggaattct actattcatc
     2521 tgattggttt caaactgctg gttgatgtta ccgaagatca tctggttgat attgcacgaa
     2581 aaagtcttat caagaatcaa gcagatttaa tcatcgcgaa tgacctgact caaatttcag
     2641 cagatcagca ccgagctata tttgttgaga aaaatcagct tcaaacagtc cagactaaag
     2701 aagaaattgc agaactcctc cttgaaaaaa ttcaagccta tcattcttag aaaggaaaac
     2761 tatggcaaac attctcttgg ctgtaacggg ttcaatcgcc tcttataagt cggcagattt
     2821 agtcagttct ctaaaaaaac aaggccatca agtcactgtc ttaatgactc aggctgctac
     2881 agagtttatc caacctttgt cactacaggt actctcacag aatcctgtcc acttggatgt
     2941 catgaaggaa ccctatcctg atcaggtcaa tcatatcgaa cttggaaaaa aagcagattt
     3001 atttatcgtg gtacctgcaa ctgctaacac tattgcaaaa ctagctcacg gctttgcgga
     3061 caacatggta accagtacag ctctagccct accaagtcat attcccaaac taatagctcc
     3121 tgctatgaat acaaaaatgt atgaccatcc agtaactcag aataatctga aaacattaga
     3181 aacctacggc tatcagctga ttgctcctaa ggaatcccta ctagcttgtg gagaccacgg
     3241 acgaggagct ttagctgacc tcacaattat tttagaaaga ataaaggaaa ctatcgatga
     3301 aaaaacgctc taatattgca cccattgcta tcttttttgc taccatgctc gtgatacact
     3361 ttctgagctc acttatcttt aacctttttc catttccaat caaaccgacc attgttcata
     3421 ttcctgtcat tattgccagc attatttatg gtccacgagt tggggttaca cttggatttt
     3481 tgatgggatt acttagcttg acggttaaca cgattacgat tctaccgaca agctacctct
     3541 tctctccctt cgtaccaaac ggaaacatct actcagctat cattgccatt gtcccacgta
     3601 ttttgattgg tttaactcct tacttagtct ataaactgat gaaaaacaag actggtctga
     3661 ttttagctgg ggcccttggt tccttgacaa atactatctt tgtccttgga ggaatcttct
     3721 tcctatttgg aaatgtttat aatggaaata tccaacttct tctggcaacc gttatctcaa
     3781 caaattcaat tgctgaattg gtcatttctg caattctaac cctagccatt gttccacgac
     3841 tacaaacctt gaaaaaataa aaacaatctc cgctttcaaa cctgaaggtg gagattttgc
     3901 tctatacagt ttctttttaa tgaaatcttt actatagtaa aacgaaataa gaacaggaca
     3961 aatcgatcag gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgt
//
LOCUS       DAVMAF010000117         3844 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMAF010000117 DAVMAF010000000
VERSION     DAVMAF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.118"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..418
                     /locus_tag="VYO97_001991"
     CDS             <1..418
                     /locus_tag="VYO97_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1754731.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VYO97_001992"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VYO97_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1754732.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGISHEKHEEDGYGFDANRII
                     AEDESGFIMSHGNHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTAGYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1440 a          626 c          750 g         1028 t
ORIGIN      
        1 taaaaataag gcagaccaag atagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaattcagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtagatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggccga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 atttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggatttat catgagtcac ggtaatcata atcattactt cttcaagaag
     2041 gacttgacag aagagcaaat taaagctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaggtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcatcatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcagcctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctggtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattatt
     3841 aata
//
LOCUS       DAVMAF010000118         3840 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMAF010000118 DAVMAF010000000
VERSION     DAVMAF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.119"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            115..1350
                     /locus_tag="VYO97_001993"
     CDS             115..1350
                     /locus_tag="VYO97_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754733.1"
                     /translation="MKLYRCWYQTFVSDEASEETPLAERGEKEYIVKSSRMTALCYAE
                     SKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRYKLVSKLEFSP
                     NLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVITRTWANNVRLS
                     AITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHEGDTLNENYDK
                     VDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPIIEDDKIWFYY
                     MGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYDDEIYILADIP
                     ENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLGNSPVAFKFEI
                     ENAKLYGIEGSIQQHYLKY"
     gene            1419..2996
                     /locus_tag="VYO97_001994"
     CDS             1419..2996
                     /locus_tag="VYO97_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1754734.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPKINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            3056..>3840
                     /locus_tag="VYO97_001995"
     CDS             3056..>3840
                     /locus_tag="VYO97_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1754735.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNIEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEK"
BASE COUNT         1370 a          523 c          754 g         1193 t
ORIGIN      
        1 aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga aaatcctaaa
       61 ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa agaattgaaa
      121 ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga gactccttta
      181 gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc actttgctat
      241 gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt aaaatttaaa
      301 ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat catgctagat
      361 gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga attttctccg
      421 aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga cgaacctatt
      481 ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag agatccaaaa
      541 actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct aagtgcaatt
      601 actttcagtg atgattttat taattggtca gaacctaaag aaatccttag gggagatgga
      661 tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta tttaggtttt
      721 gcttcaatgt accacgaagg cgacacttta aatgaaaact atgataaagt agacttagaa
      781 ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga ctatctcatt
      841 gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg tattttcacg
      901 gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg aaatggtaat
      961 cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga ccaatttgct
     1021 tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag cttccaaact
     1081 tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat taaagttgcg
     1141 gtaggtacta aatacggtaa tgcttattca gattttggtc aggaagatat ctctcttgtt
     1201 caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt agatttaggt
     1261 aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg tattgaaggt
     1321 agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa ataatctcta
     1381 tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt aataaggtat
     1441 taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt ggcaataacg
     1501 ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg tttctatcta
     1561 catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt caatggttag
     1621 aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga gtaagttcta
     1681 aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca acaggagata
     1741 agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta gatttgtcac
     1801 aacacatgga caaactacca aactataaga agtaccttga aaaaaataat gcattagctc
     1861 aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact gatgagaata
     1921 tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa tttgaagatc
     1981 tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg actgaaaaac
     2041 aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag cgagttacac
     2101 cattgtggaa tttaagccat cgtacatact atgactatga atcagattca tataaacatc
     2161 cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta cgtaaagata
     2221 acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt ctattggctt
     2281 ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat aactttgata
     2341 aatcatttga ttggcagcca cttgtttatc caaaaattaa tggaaaacgt tatttgcaac
     2401 cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca ccaaaagaag
     2461 aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat caagaaaaac
     2521 ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact caagcaggta
     2581 ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca gaactgctgt
     2641 ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt gaggcacagc
     2701 caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa gatttaggtg
     2761 gttttagacc agatactccg gttctttcct ttacaactga agaattagat gttttaacta
     2821 aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc attgaaggac
     2881 aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa gacttggata
     2941 aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg aaatagacta
     3001 aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt aagctttgta
     3061 tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg cttcggggcg
     3121 tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag ttcctattaa
     3181 tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag taaatgcaag
     3241 taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg tctttaactc
     3301 tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct atcaaccaga
     3361 agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata ttttactaaa
     3421 tattgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg aaattttaag
     3481 taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag ggaatgcaac
     3541 aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg gttgtttaat
     3601 gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa caaagcagtg
     3661 gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc gaaagtacag
     3721 agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta tgctttcaaa
     3781 agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat gggaaaaatt
//
LOCUS       DAVMAF010000119         3634 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMAF010000119 DAVMAF010000000
VERSION     DAVMAF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.120"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            81..308
                     /locus_tag="VYO97_001996"
     CDS             81..308
                     /locus_tag="VYO97_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754736.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            596..3493
                     /gene="nanA"
                     /locus_tag="VYO97_001997"
     CDS             596..3493
                     /gene="nanA"
                     /locus_tag="VYO97_001997"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1754737.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLSGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1232 a          613 c          831 g          958 t
ORIGIN      
        1 tagattgctg taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa
       61 gcttagaaaa ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa
      121 gacaagttga actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc
      181 acttatttgg gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat
      241 ttcccattat ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata
      301 aggattgagc aggaagtatg gtataaatag cataagctga tgtccatcat ttgcttataa
      361 agagatattt tagtttaatt gcagtggtgt cctggtagat aaactagatt ggcaggagtc
      421 tgattggaga aaggagaggg gaaaattggc accaatttga gatagtttgt ttagttcatt
      481 tttgtcattt aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt
      541 tctggagtaa atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa
      601 tcggagtgtt caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt
      661 ttctatgatt gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc
      721 aagtgagcaa cctctggcaa atgaaactca actttcgggg gggagctcaa ccctaactga
      781 tacagaaaag agccagcctt cttcagagac tgaactttct ggcaataagc aagaacaaga
      841 aaggaaagat aagcaagaag aaaaaattcc aagagattac tatgcacgag atttggaaaa
      901 tgtcgaaaca gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt
      961 tgatttatca agtgaactag ataaactaaa gaaacttgaa aacgcaacag ttcacatgga
     1021 gtttaagcca gatgccaagg ccccagcatt ctataatctc ttttctgtgt caagtgctac
     1081 taaaaaagat gagtacttca ctatggcagt ttacaataat actgctactc tagaggggcg
     1141 tggttcggat gggaaacagt tttacaataa ttacaacgat gcacccttaa aagttaaacc
     1201 aggtcagtgg aattctgtga ctttcacagt tgaaaaaccg acagcagaat tgccaaatgg
     1261 tcgagttcgc ctgtatgtta atggtgtttt atctcgaaca agtctgaaat ctggcaattt
     1321 cattaaagat atgccagatg taacgcatgt gcaaatcgga gcaaccaagc gtgccaacaa
     1381 tacggtttgg gggtcaaatc tacagattcg gaatctcact gtgtataatc gtgctttaac
     1441 accagaagag gtacaaaaac gtagtcaact ttttaaacgc tcagatttag aaaaaaaact
     1501 acctgaagga gcggctttaa cagagaaaac ggacatattc gaaagcgggc gtaacggtaa
     1561 accaaataaa gatggaatca agagttatcg tattccagca cttctcaaga cagataaagg
     1621 aactttgatc gcaggtgcag atgaacgccg tctccattcg agtgactggg gtgatatcgg
     1681 tatggtcatc agacgtagtg aagataatgg taaaacttgg ggtgaccgag taaccattac
     1741 caacttacgt gacaatccaa aagcttctga cccatcgatc ggttcaccag tgaatatcga
     1801 tatggtgttg gttcaagatc ctgaaaccaa acgaatcttt tctatctatg acatgttccc
     1861 agaagggaag ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca aaaaaatcga
     1921 tggaaaaacc tatcaaatcc tctatcgtga aggagaaaag ggagcttata ccattcgaga
     1981 aaatggtact gtctatacac cagatggtaa ggcgacagac tatcgcgttg ttgtagatcc
     2041 tgttaaacca gcctatagcg acaagggtga tctatacaag ggtaaccaat tactaggcaa
     2101 tatctacttc acaacaaaca aaacttctcc atttagaatt gccaaggata gctatctatg
     2161 gatgtcctac agtgatgacg acgggaagac atggtcagcg cctcaagata ttactccgat
     2221 ggtcaaagcc gattggatga aattcttggg tgtaggtcct ggaacaggaa ttgtacttcg
     2281 gaatggacct cacaagggac ggattttgat accggtttat acgactaata atgtatctca
     2341 cttaagtggc tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa aaacttggca
     2401 tgctggagaa gcggtcaacg ataaccgtca ggtagatggt caaaagatcc actcttctac
     2461 gatgaacaat gaacgtgcgc aaaatacaga atcaacggtg gtacaactaa acaatggaga
     2521 tgttaaactc tttatgcgtg gtttgactgg agatcttcag gttgctacaa gtaaagacgg
     2581 aggagtgact tgggagaagg atatcaaacg ttatccacag gttaaagatg tctatgttca
     2641 aatgtctgct atccatacga tgcacgaagg aaaagaatac atcatcctca gtaatgcagg
     2701 tggaccgaaa cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa atggtgagtt
     2761 gacttggctc aaacacaatc caattcaaaa aggagagttt gcctataatt cgctccaaga
     2821 attaggaaat ggggagtatg gcatcttgta tgaacatact gaaaaaggac aaaatgccta
     2881 taccctatca tttagaaaat ttaattggga atttttgagc aaaaatctga tttctcctac
     2941 cgaagcgaaa gtgaagcgaa ctagagagat gggcaaagga gagatgggca aaggagttat
     3001 tggcttggag ttcgactcag aagtattggt caacaaggct ccaacccttc aattggcaaa
     3061 tggtaaaaca gcgactttcc taacccagta tgatagcaag accttgttgt ttgcagtaga
     3121 taaggaagat atcggacagg aaattattgg tatagctaaa ggaagcatcg aaagtatgca
     3181 taatcttcct gtaaatctag caggtgccag agttcctggc ggagtaaatg gtagcaaagc
     3241 agcggtgcat gaagttccag aatttacagg gggagttaat ggtacagagc cagctgttca
     3301 tgaaatcgca gagtataagg gatctgattc gcttgtaact cttactacaa aagaagatta
     3361 tacttacaaa gctcctcttg ctcagcaggc acttcctgaa acaggaaaca aggagagtga
     3421 cctcctagct tcactaggac taacagcttt cttccttggt ctgtttacgc tagggaaaaa
     3481 gagagaacaa taagagaaga attctaaaca tttgattttg taaaaataga aggagatagc
     3541 aggttttcaa gcctgctatc tttttttgat gacattcagg ctgatacgaa atcataagag
     3601 gtctgaaact actttcagag tagtctgttc tata
//
LOCUS       DAVMAF010000120         3591 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMAF010000120 DAVMAF010000000
VERSION     DAVMAF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.121"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..1047
                     /locus_tag="VYO97_001998"
     CDS             <1..1047
                     /locus_tag="VYO97_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1754738.1"
                     /translation="NIGFTTPEEYGAIFKYLIENVKTDRQIIYSPHCHDDLGIAVANS
                     LAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQVETSIVLNETINTSEM
                     VSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEIITPELVGVKIPLGKLSG
                     RHAFVEKLRELALDFTEEDIKPLFAKFKALADKKQEITDADTRALVAGTMVENPEGFH
                     FDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQSVRLVSY
                     TINAVTDGIDAQDRVLVTVENRDTETIFNAAGLDFDVLKASAIAYINANTFVQKENAG
                     EMGRSVSYHDMPSV"
     gene            1059..2096
                     /gene="leuB"
                     /locus_tag="VYO97_001999"
     CDS             1059..2096
                     /gene="leuB"
                     /locus_tag="VYO97_001999"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1754739.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            2093..2362
                     /locus_tag="VYO97_002000"
     CDS             2093..2362
                     /locus_tag="VYO97_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1754740.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            2366..2725
                     /gene="leuD"
                     /locus_tag="VYO97_002001"
     CDS             2366..2725
                     /gene="leuD"
                     /locus_tag="VYO97_002001"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1754741.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            2887..3510
                     /locus_tag="VYO97_002002"
                     /pseudo
     CDS             2887..3510
                     /locus_tag="VYO97_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         1102 a          674 c          827 g          988 t
ORIGIN      
        1 aatataggat ttaccacacc agaggaatat ggtgccatct tcaaatacct gattgagaat
       61 gtcaagacgg atcgtcagat catctattcg cctcactgtc atgatgacct cggaatagca
      121 gtggcaaata gccttgctgc tgtcaagaat ggtgcaggac gtgttgaagg gactatcaat
      181 ggtattaggg agcgagctgg aaatgctgct ttggaagaaa ttgcagtggc tctcaatatt
      241 cgccaagatt actaccaagt agaaaccagt attgtcctaa atgagaccat caatacgtca
      301 gaaatggttt ctcgcttctc tggtattcca gttcctaaaa acaaagccgt cgttggtggc
      361 aataccttct cccacgaatc tggtattcac caagatggag tccttaaaaa tcctctcact
      421 tatgagatca tcacacctga attggttggt gttaagattc cgcttggaaa attatctggt
      481 cgccatgctt ttgttgagaa actgagagaa ttggccctag attttacaga agaggatatc
      541 aaaccactct ttgctaagtt caaggcactg gccgataaga agcaagaaat cacagatgca
      601 gatactcgag ctttggtagc tggaaccatg gttgaaaatc cagaaggctt ccactttgat
      661 gatttacaac ttcaaactca tgcagataat gacattgaag cgctcgttag cctagccaat
      721 atggatggtg agaaagtcga atttaatgcg acagggcaag gttccgttga agcaatcttt
      781 aacgctatcg ataagttctt taaccaatct gttcgtttgg tgtcctacac tatcaatgcg
      841 gtaacagatg gaatcgatgc ccaggatcgg gttttggtca ctgttgaaaa cagagataca
      901 gaaaccatct ttaatgcagc agggcttgat tttgatgtgt tgaaggcttc tgctattgcc
      961 tatataaacg ctaatacctt tgttcaaaaa gagaatgcag gtgagatggg acgcagtgtt
     1021 tcttaccacg atatgcctag tgtgtaaagg agaaggctat ggcaaagaaa atagtagctc
     1081 tagtaggaga cggaattggc ccagaaatca tggaggctgg tttagaagtt ctggaggctc
     1141 tagctgaaaa aacaggtttt gactatgaga ttgacagacg accgttcgga ggtgcagata
     1201 ttgatgcagc aggacctccc ttacctgatg aaacccttaa ggcaagtagg gaagcagatg
     1261 ctatcctact agcagctatc ggtagtcctc agtatgatgg agcagcggtt cgccctgaac
     1321 aaggcctgat ggctctccgt aaggaactca atctttacgc taatattcgt cctgtaaaaa
     1381 tctttgacag tctcaagtat ttgtcaccac tcaaaccgga acgaatttct ggtgtagact
     1441 ttgtcgtggt gcgtgaattg acaggcgaga tttactttgg agatcatatt cttgaagagc
     1501 gcaaagcgcg tgatatcaac gactatagct atgaggaagt ggagcggatt attcgcaaag
     1561 cctttgaaat tgcaagaaat cgcagaaaaa tcgttactag tatcgataag caaaatgttc
     1621 tagcgacctc aaaactctgg cggaaagtag ctgaggaagt cgcacaggat ttcccagatg
     1681 taaccttgga acaccagctg gtagactcag ctgctatgct tatgattacc aatcctgcta
     1741 agtttgatgt tattgtaacg gagaatcttt ttggagatat tttatctgat gaatcaagcg
     1801 tcttatctgg tacacttgag gttatgccat cagccagtca ttctgaaaat ggaccaagtc
     1861 tctatgaacc tattcacggt tcagcacctg atattgcagg tcaaggaatt gccaatccta
     1921 cttccatgat tttatcagtt gccatgatgt tgagagatag tttcggacgt tatgaggatg
     1981 cagagcgtat caaacgtgct gttgagacaa gtctggcggc aggaatttta acgagagata
     2041 taggaggtca ggcttcaaca aaggaaatga cggaagctat tattgcaagg ttatgaagtt
     2101 agacgaaaaa attactctag tccttttgat ttggaatgtc atcattttct tgatttatgg
     2161 cattgacaaa ttcaaggcaa gaagaagaac ttggcgcatc caagagaaaa tcttactcat
     2221 tttagccctt acttgtggtg gttttggggc ctggttagct ggaatcactt ttcaccacaa
     2281 gactcgaaaa tggtacttta aaacagtttg gtttcttggg atggtgacca cactagtagc
     2341 cttatatttt atttggaggt aatggatggc agggtcttcg agggaacacg ctgcttgggc
     2401 tctagcggac tatggtttta aggtcgtgat tgcaggatct ttcggtgaca ttcattacaa
     2461 taatgaactc aataatggca tgttgccaat cgttcagcct agagaggtta gagagaaact
     2521 agcccagcta aaaccaaccg accaggtaac tgtggacttg gaacaacaaa aaatcatctc
     2581 accagttgaa gaattcacct tcgagataga tagcaagtgg aaacataaac tcctaaatag
     2641 tttggatgat atcggtatta ccttgcagta tgaagagttg attgctgctt atgaaaaaca
     2701 acgaccagcc tactggcagg attagaaaaa atagaaaagg aaatatagta aactgaaata
     2761 agatgtaaac aaatgaattg gagcttaaca tccatttcca gcaatttttt agaaactaca
     2821 gtggactatt ctggattcaa cacattataa aattatgaca aaacacattc acaagaaggc
     2881 tacgacattt taaaaggtga gggcggatgt atcgtttgcc ctactaaagt tggttacatt
     2941 atcatgacca gcaacaaggc aggacttgag cgtaagttcg cagccaaaga acgtaagcgt
     3001 aacaaaccag gtgttgttct ctgcggtagc atggatgaac ttcgcgcttt agcgcaactc
     3061 aacccagaaa ttgaagcatt ctactaaaaa cattggatga agatattctt cttggttgta
     3121 tccttccttg gaaaccagaa gcctttgaaa aactcaaagc atacggggat ggccgtgaag
     3181 aacttatgac tgatgtacgt ggtactagct gttttgttat caagtttgga aaagcaggtg
     3241 aacaattggc tgccaagctt tgggaagaag gtaaaatggt ctacgcctca tatgcttcaa
     3301 tgacaaaacg attgaaactc gctatgagag caaggtgtaa tggtgtctat ggtcgataag
     3361 gacggcaaac tcatcccaga acaaggagga gcacgttcaa cttcaccagc accagttgtg
     3421 atccgtaaag ggcttgacat tgataaaatc atgatgcacc tgtcagatac ttttaactca
     3481 tgggactacc gtcaggttga gtattattaa gataaaaaaa gaagtctagt gttatgagat
     3541 attaaagctc ctaacactgg actttttgtt tagaatttct tttctttttc t
//
LOCUS       DAVMAF010000121         3533 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMAF010000121 DAVMAF010000000
VERSION     DAVMAF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.122"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..125)
                     /locus_tag="VYO97_002003"
     CDS             complement(<1..125)
                     /locus_tag="VYO97_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1754742.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKL"
     gene            complement(311..922)
                     /gene="rpsD"
                     /locus_tag="VYO97_002004"
     CDS             complement(311..922)
                     /gene="rpsD"
                     /locus_tag="VYO97_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1754743.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1117..2169)
                     /locus_tag="VYO97_002005"
     CDS             complement(1117..2169)
                     /locus_tag="VYO97_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1754744.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQMETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2166..2864)
                     /locus_tag="VYO97_002006"
     CDS             complement(2166..2864)
                     /locus_tag="VYO97_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1754745.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQI"
     gene            complement(3031..>3533)
                     /locus_tag="VYO97_002007"
     CDS             complement(3031..>3533)
                     /locus_tag="VYO97_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1754746.1"
                     /translation="QLALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDL
                     DNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          980 a          786 c          680 g         1087 t
ORIGIN      
        1 tttagcttta accagccata aatggtatta cgtgagattt ggaaaacgtg tgatgcttct
       61 gttatactac ctgttcgctc acaataagag agaacttttt tacgaaaatc tattgaatat
      121 gccataaaaa gattatacca cattgtgtac tatttttggt tcattttact atatcacttt
      181 ggactatatc ggtctagtta tgtctaaacg attatagctg gatactaaga gaaatcaaaa
      241 aagcactaag ggagaacgtc ccaaagtgct taatatcaag gcctcaaagc ctgtcttatt
      301 caataaaata ttacaacatc ttgttgtaga attcaacgac aagtgcttcg ttgatttctg
      361 ggttgatttc gtcgcgttct ggcaagcgag tcaatgaacc ttccaacttt tcagcgtcga
      421 atgatacgaa tgctggacgt ccaagagtag cttctactgc ttcaaggatt gctggaactt
      481 tcaatgattt ttcacgaact gagatcactt gacctggagt tacgcggtat gatgggatat
      541 caacgcgttt cccgtcaaca aggatgtgac cgtggtttac gaattgacga gcttgacgac
      601 gagtagtcgc gagaccaaga cggtaaacaa cgttatccaa acgacgttcc aaaagaagca
      661 taaagttgaa acctaggatt ccgcctttga tttttgtagc ttgtacgaac aagttacgga
      721 attgtttttc acctacaccg taagtgaaac gaagtttttg tttttcagcc aattgcaaac
      781 cgtattctga caatttagaa cggttgtttg gtccgtgttg tcctggtacg tagttacgac
      841 gtgccaattc tttacctgta cctgtaagtg aaaggccaag gcgacgagct tgtttccaag
      901 atggtcctgt ataacgtgac atatgaatgt cctcctgata taaataatat ttcggtggaa
      961 atagtcactt agaaagccct gattcgtgca gatgcccttc gcctaaacag ccaaggttac
     1021 ttatcataag acacctgttg acgagcttca tgctttcctg ctgatatttc acacaaggtc
     1081 cattttacca tgaatagctg gatttgtaaa ggggttttag gctttatttt cactaccaga
     1141 gaggttgaga acgagggtaa aggtacttcc tagaccgtac tggctgctga ctgtgatttc
     1201 cccacccaat tgatgggcca attcacgcgc aatcgcaagt cctaatccat gaccacctgt
     1261 cttcatgtta cgcgaagttt cgacacgata aaggcgtttg aaaatatttt ccaaatcctc
     1321 tggggcaatg ccctgccctt catcggtcac actgattgaa agctggccct tctccagctt
     1381 agccaccact tccagcttgg ttcctggagc agaatattta aaagcgttat cgaccagatt
     1441 caccaagata cgagaaagct tagcataatc tccctcaatc cgggcagact ctgggattac
     1501 ctgcaagtgg acatctcttc tctcctgctc aatcaaaaac tgaaattcac tcatgcactc
     1561 aattaagagc ttgtccagaa aaatactgtc tttactggta gtttccatct gatttctagc
     1621 tgtgtttagg gtcaaaaaat tcaactcctc aaccaattta ttgagcctct ccgtctggcg
     1681 tccaatggtt gctagataat gagcttgctc cgactccttg ataatcccat ccaaaatccc
     1741 ttctaccgtc gcttggatcg aagtgatagg ggtcttaata tcatgcgaca actgggcaat
     1801 catcaagccc ttttctcgtt cgctttcttc caaggaatca aagcttacct gcaaatcatg
     1861 ggacatctca ttaaaagttt gccctaattg ctgaaattct acaggacctt gaacctccaa
     1921 atttgaagga aaatccttgg ccgctacccg cttggcatgc tccttgagtt tgcccaacga
     1981 cgtaaagact ggcaatagga gaaagagact aatcccagca ccgacaaggc tggcaacgat
     2041 ggtcatccca agcaaaaagt aaatctcgcc tttcgcaatc agcatttttt gaacagccca
     2101 aaaaacaacc aaaatggtta agagggttga aataatatat ccaaccaaaa tataactttt
     2161 tagtttcata tttgtcctct cggtttctct atcttatatc ccaaccccca aactgtctta
     2221 atagtgggag tttggtcact actatatttt gccagctcct gtcgaagagc atggatatgc
     2281 acattcaagg tattggtgtc atccacgtag tcttctttcc agatcttttc atagaggtct
     2341 gtcttggaga aaactcgctc tggattgcta gctaaaatcc acagcaattc aaatgattta
     2401 acagttaaat ccagcatttc ttctcctatt tgaacttcat gactactatg attcattttt
     2461 agattgccaa gggaaatcag ctctgtttcg cccccacgat gaaggcggcg caaaatattg
     2521 tggacacgca aaaccagctc acgtgggcta aaaggcttag caataaaatc atctgctccc
     2581 aagctcaggc cgtaaatctt gtcctgttca ctggtcttag cagtaataaa taggaaaggc
     2641 tgctctggtg ataagtattg aacctcactg attaaatcat aaccatccat ccgaggcatc
     2701 atgacatctg tgataatcaa atcaatcggt tttttcttga acagctctag cgcttccagt
     2761 ccatcatggg ctaccaagac ctgataacct gcctgaatta agtatctctg atgaatatct
     2821 gtgatttcta cctcgtcgtc aacgagtaaa attgtctttc ccatctgtct ctcctttgat
     2881 aaaaacagtg ctatactgct ttaagtataa cactattttt attgttttac gatgatttta
     2941 atctgctatt gaaagaaagt cctgattgct gattgagttg ttaaactaat cttagtcagc
     3001 ccactatcca tcatttattt ccttagctga ttagttttct tcttttttgt ttttcaaacc
     3061 tagaccaccg agtaaacctg caagcgcaag cccaaggaaa ccaatacttg ccattgatgt
     3121 ttgagtctca ccagtatttg gtagcatagc tttatcctct gacatcatcg tatcagacat
     3181 cttgttagca gaagcagcca tgttttcacc tgccatcgtg ttggtagaac ttgtcatggt
     3241 gtcagcaggc atgctatctg tagtacctgt agcatgattg tgattcatcg gagtcacgcc
     3301 agaaccagag ttagaaggtg ataatgaacc atttgctgtg tctgatgttt ctttaacatt
     3361 tatcttaata gtgacttttt tagttgctac gatgttgtcc aaatctggtt taccgtcttt
     3421 gttaccatag acattgactg tagcgctgta agtttgagta ccatttgctc ggaactggtc
     3481 aatgagcgct tgtttttctt tgccagctac atttccgtcc aaggctaatt gat
//
LOCUS       DAVMAF010000122         3504 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMAF010000122 DAVMAF010000000
VERSION     DAVMAF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.123"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..1032)
                     /gene="phtA"
                     /locus_tag="VYO97_002008"
     CDS             complement(<1..1032)
                     /gene="phtA"
                     /locus_tag="VYO97_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1754747.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNISNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELE"
     gene            complement(1286..3019)
                     /gene="ptsP"
                     /locus_tag="VYO97_002009"
     CDS             complement(1286..3019)
                     /gene="ptsP"
                     /locus_tag="VYO97_002009"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1754748.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(3025..3288)
                     /locus_tag="VYO97_002010"
     CDS             complement(3025..3288)
                     /locus_tag="VYO97_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1754749.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          920 a          755 c          673 g         1156 t
ORIGIN      
        1 ttccaattca gacatttgtt cataagggat aaagtggtaa tggttaccat gagggacagc
       61 tacacctctg gcggttcgac ttgtgatttg cgctgggtcg aaaacaaggc catcagattc
      121 tacatggcgt tgactcaaag gcagtttgta gagctgtttc aagagactat caatgtcatt
      181 actttgactt gcttgactgt tagtgttgct gttgttgctt atgttagtat ttgtagttcc
      241 tggattgctt acagaaggta cccagtttgt tcttgaagtg ttatcgctat tttgtcggcg
      301 ataggttctt gaatttgaca gatttccccg accagatagg aaggcttctg cagcagctaa
      361 ctcgctagct gataactcat tcttaggaat gtaatggtaa tggtcgccgt gaggaacgat
      421 ataagcatca cccgtgtcct caatgatatc agaagcatta aagatataac catcatctgt
      481 ggtgtagcgt ccctgtgaac gtgcaaaggc taccgcacca tcgtttgctg aagtccctcc
      541 ttcacgatgc tgactatgtt cttgtttttg ccgattgatt tcttcttttg tacggacatt
      601 atccgcgtgg gcagcatcct taaggtaaac atagtatttt ccatctacct tgataacata
      661 tccacccttg acctcattaa caatatcctc atcttttagc ttatagtttg gatctttcat
      721 gagtaattct tcactgaaaa tcgcatcata aggaacctta ccattgtaat aatgatagtg
      781 gtcgccatgt gaagtgacat agccttggtc tgttatcttg atgacgattt gctcagcatt
      841 gattccttca cgcttgctaa cctcatcagg agtcaaattc tccgtttttt gcgtcgcttg
      901 ttttccatct atataggaaa cacgattatt ttccttaacc gttctagctt gatacagtcc
      961 caactcgtaa gaacaaacac ttaaaatcaa agctgccgca gaaccaacaa ggtatttctt
     1021 attaattttc atcgaaactc tctttctatt tgatatttct agttgcaatt catttctaaa
     1081 ttgcagaaac tcctctctat tacttaactg gttaatagtt tactcctaaa tctccaactt
     1141 gtcaagaatt ttatgaacca tttaagtatt ttttgttctg accactactt tttcaaggtt
     1201 ggactttcta taaaaaagat tttcttgaaa attcttttta aaatatcaaa aaaatccctg
     1261 taactgagtt gcagggactt ttcgattaat caaaattaac gtattctttt tgaagttcaa
     1321 gaacttcttc cattgttgag cattctgtaa gggcacggtt tgcgtactct tccatcttag
     1381 ctgtgtcgag tttcttcatc aagctgcgtg tacgaagtac agatgttgct gacatagaga
     1441 actcatccaa gcccattccg acaagaagtg gaacagcttg ttggtcacca gccatctcac
     1501 cacacatacc agcccattta ccttcagcgt gagctgcttt gatcacattg ttaatcaagc
     1561 gtaggattga tgggttgtat ggttggtaaa ggtatgaaac ttgttcgttc atacggtctg
     1621 ctgccattgt atattggatc aagtcgtttg taccaattga gaagaagtca acttctttag
     1681 caaattggtc tgcaagcata gccgctgcag gaatctcgat catgatacca acttggatgt
     1741 tatccgcaac tgcaacacct tcagcaagaa ggtttgcttt ttcttcatca aagactgctt
     1801 tcgctgcacg gaattctttc aagagcgcaa ccattgggaa catgatacgc aattgaccgt
     1861 gaacagacgc acgaagaaga gcacggattt gtgtgcggaa catagcatct ccagtctcag
     1921 agatagagat acgaagagca cggaatccaa ggaatgggtt catttcgtga ggcatatcga
     1981 agtaaggaag ttccttatct ccaccgatat ccattgtacg aacgacaaca ggtttaccgt
     2041 tcattccttc aagaacagcc ttgtatgctt catactgctc atcttcagtt gggaagtctt
     2101 gagaatccat gtacaagaac tctgtacggt aaagtccaac agcttctgca ccgttgttgt
     2161 taacaccttc aacgtctttt ggagtaccaa tattggcagc caactcgaag tgtttaccgt
     2221 cagcagtcac tgtttgagca tctttcaaaa gtgcccattc agctttttgt ttcgcatagg
     2281 cttcaccagc tgctttaaat tctgccgctt gttcatctgt tgggttgata atcacttctc
     2341 cagtgatccc gttaacagca aggatgtcac cgtctttaac gatttcagtg atgttatttg
     2401 tacctaatac agcagcaatt tcaagtgtac gtgccatgat agctgagtgg cttgtacgtc
     2461 caccaatgtt ggttacaaaa gcttttacaa agtttttgtc caattgagct gtatctgaag
     2521 gagtcaagtc atgcgcaatc acaatcactt cttcattgat agaagctggg tttggcaatt
     2581 ttttaccaag aaggtttgcc aatacacgtt ttgtcacgtc gcggatatcc gctgcgcgtt
     2641 cttgcatgta tgggttgtct tccatgcctt caaagatagt gataaacata tctgtaactt
     2701 ctttcagacc tgcttctgca ttcactttct tcgcacggat agtttccttg atttggctga
     2761 tcatttctgg gtcagcaaga accattaagt gagcatcaaa aacttgagct gcttcttcac
     2821 cgagcgtacc tactgctttc tcgcgaataa cagaaagctc gtcttgtgat gcctgtagag
     2881 cggcatcaag gcgagcttct tctgcgtttg tatcttcgac tgtaatagtc tcaaatgaca
     2941 aatccggctg aacgagtaga tatgcttttg caactgcaac accgtcagat gctgcgattc
     3001 ctttaagcat ttctgtcatt tcccttatgc caatccttct ttttccattg tttctgagat
     3061 tgcagcgata gcgtcatctg catctgcacc ttcagctgag atagttacgt cagcaccttg
     3121 gccaacacca agactcataa cacccataat tgatttaagg ttaactgatt tacctttgta
     3181 ctcaagagtg atatctgaag caaatttgct agcagtttgt accaacaatg ttgctggacg
     3241 tgcgtgaata cctgtttctg ccactacgtg gaaatcttta gaagccatag tttgactctc
     3301 cttttgttct ttcttttttt gagttatatg tgataaccct tacaatttag tattatacca
     3361 tcttctagga tttttttcaa gcattttctg gattttcctt caatttcctt tcagataact
     3421 tgctgaaaat cttctatctt agagaacaaa acacaggata tagtttcttc aaaaacaatt
     3481 tataggataa tcattgctat ttca
//
LOCUS       DAVMAF010000123         3452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMAF010000123 DAVMAF010000000
VERSION     DAVMAF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.124"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..2216)
                     /locus_tag="VYO97_002011"
     CDS             complement(<1..2216)
                     /locus_tag="VYO97_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754750.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAVFRPEGIERIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQAG"
     gene            complement(2203..2553)
                     /locus_tag="VYO97_002012"
     CDS             complement(2203..2553)
                     /locus_tag="VYO97_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754751.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            complement(2592..2972)
                     /locus_tag="VYO97_002013"
     CDS             complement(2592..2972)
                     /locus_tag="VYO97_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754752.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            complement(2977..3390)
                     /locus_tag="VYO97_002014"
     CDS             complement(2977..3390)
                     /locus_tag="VYO97_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1754753.1"
                     /translation="MLANGITLSYGEAKGTYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVNFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSELEFTDGLGG"
BASE COUNT          947 a          858 c          611 g         1036 t
ORIGIN      
        1 ccggcttgtt cagcccatga taagaaatta atcgtttgcc aagttgttat caaagccaag
       61 ataggttctt tgttttcttt acaccagtca gaaaaaacgg tgaaaccatc tgccactaac
      121 ttaatagcat ccgccaatag tcccaaagtg gctaaaaggc cacctcctaa taaatctgaa
      181 attccttcaa tactaacacc gaatactcct gataaaaact cagcaaaagg ttgccaagaa
      241 ttctcccaga ggatttgtat gatatcaatc agtccgttaa aagcattagc aatagagtta
      301 atagcagggg ctacatgttc atcataaaca cgacttaagc catcgccaaa tttgttaaca
      361 gacctttcaa tgctctcaaa tacaggcgca acagtatcta ataaactttg gaagactgat
      421 gaaattttag gagcgcttgt cacaacgact ttttcaaaac ctttaaacaa acttcctgct
      481 aatttactac caacttcaac aatggtagat gtcaaactca acagagttga cacaatagcg
      541 ctaccgatac gaaccgcacc agttgaggta atgacgtcgt agaaagtact agaaaagtcc
      601 tgagcgatgt ttcctactgc ctcggaaatg ttaccaatat tatcaaacaa agcgactagc
      661 gccctggtaa tgcgttcttt ttgccttcca aggccatttg caatactttc ggcaaggaaa
      721 acaccgatac ctagcccgat agttgttatt gagcctgtca cttgccctaa agcataagca
      781 attttctcag ccattcggtt aaaggcattc acaacccttg ggtcagtggc gatttctccc
      841 attgtcttag ctatttggtc taaggcagtc ttaatgcgtt ctataccttc tggtctaaat
      901 actgcatcaa aacctttctt gaagaggtca aacaaccctt tgagcttatc tccaagacca
      961 tcaaaaatgc tcttgaattt gttgtccatg tcggtcaact cgacttctgg caagatgtct
     1021 ttgaaaggtc cgccaccgcc tccctttcct ttaccacctt tgccaccgcc tccagacccg
     1081 cctgcgtcgt catcttttgg tttttgcaag atgttaatct catcaaatcc caaaagacct
     1141 agcaactctt tagcggcctt cttagcgttt ttggcggagt ctccaagatt gtcagcaagt
     1201 cctcctgctg aatctccagc gtcgtctact gcgtcagcaa ggtctcctgc tccgcctgca
     1261 gcatccttca tggcgttacc catgtctcca actgctccac caacaccatc tttcactgtt
     1321 gctttcttgt tgaacatcaa agcgataaac tcagcgagtt tagcagtaac gttctttaag
     1381 accatagcaa aagagttcaa gacaggcata atggcattga taatcggtaa catagagtta
     1441 ccaaggttca atgctgcgtc cttcatcagc gacttaaata ggctgatacg accatttaca
     1501 gaattagaca aggtatcccc atacttggct gtagcctgtt ccagaatagc cataaggcgg
     1561 atttgttgct gggtttggta atccaactgt tgccagctct gtccgtttgc gaacttctta
     1621 aaggcttcag tggactcaat catagccacg ttgacgttga ttcctaggtc ctcaatcgct
     1681 tcggtgttcc ctagtaaacc tgagcgaatc cgctccataa cgtctgtaat cgtgcgccct
     1741 gaaccttcag caacaactgc cgatgtctgc aacatcttag cggtataggc gcttagcttg
     1801 ttggtatctt tgataaatcc agaaaataag tttgagtaga ctgcaccgta gttggtagcc
     1861 tcacccacac ccatattcat agcgttggca ttatcgttaa cccattttaa gaaagattgc
     1921 gaactctcgc ccatctgtcg cttgatttgg ttcatagacg ctgatacttc aagagccgtc
     1981 tgcgttgaat acatcccaac atcaagtaat ttcttaccaa ggattgcaaa accagcgaac
     2041 ttagccagct tgccaaacgc actaccgatt gagctcgact gttcacgaac tttggcagta
     2101 gcatttttca cttggtcaga tgttcctttg acctgattct cgacttcttt catcttcttc
     2161 ctgaaaggcg ctatctcagc gtcaatcatg actttcaatt catcaagagt tgccatttac
     2221 ttcctccttc ctttttcgat tatgtctctc tgcaaattca cgcatccgtt ccttatgcaa
     2281 caaaagtgct tgtttctgtc gttcctgttc tactgcttgt tgttcttcta caaacaactc
     2341 aggcgcatat tcccagaact caaaggcctt ggcatctttg gataacaata aagaaacgtg
     2401 gttggatatc atctgcgaaa gtctgtatga gtcaataatc ttttctttac gctcttggat
     2461 tttgacacgg ttatagcttt caatcatttc tctgatttca agtaccgtca aatcccaaaa
     2521 atcaagaggc ttgcccccga tgtccaaaaa cataggataa agcctctcaa taatctgagt
     2581 taccgttaag attacttgtc tactgtcatt ttcttcttgg aagttttctt gtccttgctt
     2641 cctcgtggag taaaacccga tacttcaaag agtggcatca acacctctgt catgaaggtt
     2701 gtttggtctc cgccattgtc cacgtattca tcgtataaat catagacatc ctcaagagaa
     2761 tacccatgtt catactgctg caaggctccg tgaactaaca acaacataac tttcaaaggc
     2821 ggtaaagtga actcttcgcc agcttcaggc atgaaaatct tcagcaagtt catgccgatt
     2881 ttttcttcca cagttgcagc ttgatgagat gtcaaacgta gcttcaactc tttttcgtca
     2941 gtaactttcc aagttgtgta ttttaacgcc atttaattaa cctccaagac cgtctgtaaa
     3001 ttccaactct gactgcaacg caattttaag ggtgaactcg ataacggcat tgacaccgcc
     3061 accgccaagc ttaacagata cttggccttc aaaattgacc ttagtaccgt ctgggtatgc
     3121 ttgctcgaag tagagtttct tcttgtcgtc tgctgcctta cgcaatacac gataaggagc
     3181 agttgcgctt gaattattat aagagaactt gtactcaagt tctcctatgt ccccaatacc
     3241 aaactcgtac ttcttcactg tatcttcaag agtagtattt tctacttttt cgagttcaat
     3301 accaaactct ggcacttctt tcaatccaac aagtttagta taagttcctt tagcttcgcc
     3361 ataagatagc gtaattccat ttgctaacat gtttaattct ccattctaaa ttgaaaaaca
     3421 agctctgagt ctaaatcaac gacaccttca aa
//
LOCUS       DAVMAF010000124         3452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMAF010000124 DAVMAF010000000
VERSION     DAVMAF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.125"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            63..476
                     /locus_tag="VYO97_002015"
     CDS             63..476
                     /locus_tag="VYO97_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1754754.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELEYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVTFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSNLTFTDGVGG"
     gene            481..861
                     /locus_tag="VYO97_002016"
     CDS             481..861
                     /locus_tag="VYO97_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754755.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVE"
     gene            900..1250
                     /locus_tag="VYO97_002017"
     CDS             900..1250
                     /locus_tag="VYO97_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754756.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIKSYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEINGNS"
     gene            1237..>3452
                     /locus_tag="VYO97_002018"
     CDS             1237..>3452
                     /locus_tag="VYO97_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754757.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RSITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERIIRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFENIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAG"
BASE COUNT         1032 a          609 c          863 g          948 t
ORIGIN      
        1 tttgaaggtg tcgttgattt agactcagag cttgtttttc aatttagaat ggagaattaa
       61 acatgttagc aaatggaatt aaattggcct ttagtgaaac gaaaggcaat tatcagaatc
      121 ttgtagggct taaggaagta cctgaatttg gtatcgaacc cgaaaaagta gagaatacta
      181 cccttgcaga tacagtgaag aagtacgagt ttggtatcgg ggacgcagga gaacttgagt
      241 acaagttcgc ttataataat tcaagcgcaa cagctcctta ccgtgtatta cgtaaggcag
      301 cagatggtaa gaaaaaactt tactttgagc aaacatatcc agatggtact aaggtcactt
      361 ttgaaggtca agtatctgtt aagcttggcg gtggcggtgt caatgccgtt atcgagttca
      421 cacttaagat tgctttgcag tctaatctta catttactga tggtgttgga ggttaattaa
      481 atggcgttaa aatacacaac ttggaaagtt actgacgaaa aagagttgaa gctacgtttg
      541 acatctcatc aagctgcaac tgtggaagaa aaaatcggca tgaacttgct gaagattttc
      601 atgcctgaag ctggcgaaga gttcacttta ccgcctttga aagttatgtt gttgttagtt
      661 cacggcgcct tgcagcagta cgaacatggg tattcctttg aagatgtcta cgacctatac
      721 gatgaatacg tggataacgg cggagaccaa acgacattca tgacagaggt gttgatgccg
      781 ctatttgaag tatcgggttt tactccacga ggaagcaagg acaagaaaac ttccaagaag
      841 aaaatgacag tagtcgagta atcttaacgg taacgcagat tattgagagg ctttatccta
      901 tgtttttaga catcgggggc aagcctcttg atttttggga tttaacggtg cttgaaatca
      961 gggaaatgat aaaaagctac aaccgtgtca aaatccaaga gcgtaaagaa aagattattg
     1021 actcatacag actttcgcag atgatatcca accacgtttc tttattgtta tccaaagatg
     1081 ccaaggcctt tgagttctgg gaatatgcgc ctgagttgtt tgtagaagaa caacaagcag
     1141 tagaacagga acgacagaga caagcgcttt tgttgcataa ggaacggatg cgtgattttg
     1201 cagagagaca caatcgaaaa aggaaggagg aaataaatgg caactcttga tgaattgaag
     1261 gtcatgattg acgctgagat agcgcctttc aggaagaaga tgaaagaagt tgagaatcag
     1321 gtcaaaggaa catctgacca agtgaaaaat gccactgcta aagttcgtga acagtcgaac
     1381 tcaatcggta gtgcgtttgg taagctggct aagttcgctg gttttgcaat ccttggtaag
     1441 aaattacttg atgttgggat gtattcaacg cagacggctc ttgaagtatc agcgtctatg
     1501 aaccaaatca agcgacagat gggagagagt tcgcaatctt tcttaaaatg ggttaacgat
     1561 aacgccaacg ctatgaatat gggggtgggt gaggctacta actacggtgc ggtctactca
     1621 aacttatttt ctgggtttat caaagatacc aacaagctaa gcgcctatac cgctaagatg
     1681 ttgcagacat cggcagtggt tgctgaaggc tcagggcgta gcattacaga cgttatggag
     1741 cggattcgct caggtttgct agggaacacc gaagcgattg aggatctagg aatcaacgtc
     1801 aatgtggcta tgattgagtc cactgaagcc tttaagaagt tcgcaaacgg acaaagctgg
     1861 caacaattag actaccaaac ccagcaacaa atccgcctta tggctattct ggaacaggct
     1921 acagccaagt atgggaatac cttgtctaat tctgtaaatg gtcgtatcag cctgtttaag
     1981 tcgctgatga aggacgcagc attgaacctt ggtaactcta tgttaccgat tatcaatgcc
     2041 attatgcctg tcttgaactc ttttgctatg gttttgaaga acgtgacggc taaactcgct
     2101 gagtttatcg ctttgatgtt caacaagaaa gcaacagtga aagatggtgt tggtggagca
     2161 gttggagaca tgggtaacgc catgaaagac gctgcaggcg gagcaggaga ccttgctgac
     2221 gcagtagacg acgctggaga ttcagcagga ggacttgctg acaatcttgg agactccgcc
     2281 aaaaacgcta agaaggccgc taaagagttg ctaggtcttt tgggatttga tgagattaac
     2341 atcttgcaaa aaccaaaaga tgacgatgca ggcggttctg gaggcggtgg caaaggtggt
     2401 aaaggaaagg gaggcggtgg cggacctttc aaagacatct tgccagaagt cgagttgacc
     2461 gacatggaca acaaattcaa gagcattttt gatggtcttg gagataagct caaagggttg
     2521 tttgacctct tcaagaaagg ttttgatgca gcatttagac cagaaggtat aaaacgcatt
     2581 aagactgcct tagaccaaat agctaagaca atgggagaaa tcgccactga cccaagggtt
     2641 gtgaatgcct ttaaccgcat ggctgagaaa attgcttatg ctttagggca agtgacaggc
     2701 tcaataacca ctatcgggct aggtatcggt gttttccttg ccgaaagtat tgcaaatggc
     2761 cttggaaggc aaaaagaacg cattatcagg gcgctagtcg ctttgtttga taatgttggt
     2821 aacctttccg aggcagtagg aaacatagct caggactttt ctagtgcttt ctacgacgtc
     2881 attacctcaa ctggtgcggt tcgtatcggt agcgctattg tgtcaactct gttgagtttg
     2941 acatctacca ttgttgaagt tggtagtaaa ttagcaggaa gtttgtttaa aggttttgaa
     3001 aaagtcgttg tgacaagcgc tcctaaaatt tcatcagtct tccaaagttt attagatact
     3061 gttgcgcctg tatttgagaa cattgaaagg tctgttaaca aatttggcga tggcttaagt
     3121 cgtgtttatg atgaacatgt agtccctgct attaactcta ttgctaatgc ttttaatggg
     3181 ctaattgaca ttattcagat tctctgggag aattcctggc aaccttttgc tgagttttta
     3241 tcaggagtat tcggtgttag tattgaagga atttcagatt tattaggagg tggcctttta
     3301 gccactttgg gactattggc ggatgctatt aagttagtgg cagatggttt caccgttttt
     3361 tctgactggt gtaaagaaaa caaagaacct atcgtagctt tgataacaac ttggcaaacg
     3421 attaatttct tatcatgggc agaacaagct gg
//
LOCUS       DAVMAF010000125         3400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMAF010000125 DAVMAF010000000
VERSION     DAVMAF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.126"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..721)
                     /locus_tag="VYO97_002019"
     CDS             complement(<1..721)
                     /locus_tag="VYO97_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1754758.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEK"
     gene            881..2635
                     /locus_tag="VYO97_002020"
     CDS             881..2635
                     /locus_tag="VYO97_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754759.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            2619..>3400
                     /locus_tag="VYO97_002021"
     CDS             2619..>3400
                     /locus_tag="VYO97_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754760.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVG"
BASE COUNT         1195 a          484 c          573 g         1148 t
ORIGIN      
        1 ttttttcaag cgtgccttga tttacattcg ttgcaaaatg atcatctaag aatcttgcta
       61 catcttcaag tgctttatca tcatcaattt caattttata tttttttcta tttaagtatg
      121 tgtcaattac tatacttatc cattcatttg cctttgcttt aaagaaaaaa tcagcggcag
      181 gagcgtccat cttacaattt aatatttcca ttgccactct ttctaaggcc tttgtaagta
      241 ttatttgatt cggttgaagc aaggttgaat aaaaagattc tggattaatg ttaatagatg
      301 ctaaatgttt ttctattagc tcttttttaa aaccaatgga aacagcaaga taacaacaat
      361 tctcgtgtaa taaaaaaaca aaatcatctt ttatattatc aaaatcaaaa gtacataaag
      421 agtttgcggt aatagtttga tacggattaa acttttctcc gtttgcactg acaatgtaac
      481 ttgaataaat tgaaacatag tctgacatac tataagtgct attttgaact acttcctctt
      541 tgatataaaa atcatgtata tcgataatga agctgcctcc ttcataaaac caatataggc
      601 cttctgcata agttgaattc tcttttttcc aacaaaatgt atgacctgca cttgaatact
      661 tctcattgtt tttacattca tctactttca tataatcttt tacaaaatca taatatgaca
      721 taacacacta tcccttttag acaatattcc aattagcctt attaattcaa aactattgta
      781 ttagtaatta taacagatgt ataatagaaa agcaatgata gatattatca attaagcgaa
      841 tttatatcta aaagggatat taaagaaagg agatatgctt atgaagattt acaaaaaact
      901 atttgcttat gtccaagata agaaatatct tggggttttg gccataattt tttctgctat
      961 atctgctcta cttacagtat atggatatta tttaatctac aaatttctag ataagttaat
     1021 aattaattca aatttatccg gtgcagagag catagcatta aaatctgtta ttacactaac
     1081 aagtggagcg atattttatt ttgtctcagg aatgttttca catatcttgg gattcaggct
     1141 tgaaacaaat ttaagaaaaa ggggaatcga tggtctggaa aaagcaagtt ttaggttctt
     1201 tgacttaaat ccatctggtc aaataagaaa gattatagat gacaatgctg cacaaactca
     1261 tcaggtggta gcacacatga ttcccgatag ttctcaggca ataatcacac ccgtacttgt
     1321 acttgcactt ggctttatag taagtataag agttggcata attttgcttg ctcttactat
     1381 aattggtggc ttaattttag gggcgatgat gggcgagcaa gaatttatga agatatacca
     1441 agaatcccta tctaaactaa gtgctgaaac tgttgagtac gtgagaggaa tgcaagttgt
     1501 aaaaatattt aaagcaaatg tagagtcatt taaaagcttt tacaaggcta taaaagatta
     1561 ctcaaagtat gcttatgatt attccctatc ttgtaaaagg ccttatgttt tgtatcaatg
     1621 gttatttttt ggactgattg caattttaat tattcctata gtttatttta tgactagctt
     1681 agctagcgca aaggtgattt tacttgagct tatcatgatt ttatttttat caggagttct
     1741 ctttgtttca ttcatgagaa tgatgtggta ctccatgtat atttctcaag gaaattatgc
     1801 agtagatact ttagaggcgc tttacgaaga tatgcaaaaa gacaaattag tgcatggtaa
     1861 tgtcaataat tttaaaaact ataatataga atttgagaat gttagctttg cttataatga
     1921 taaagctgtc attgaaaatt tatcctttaa tttagaagaa ggaaagtcct acgcacttgt
     1981 cggttcatct ggatcaggca aatcaacagt agcaaaactt atatcaggtt tttacaatgt
     2041 taataaagga agcataaaga taggcgggat agcaataagt gaatattctg acgaagcctt
     2101 aattaaagcc atttcctttg tttttcaaga ttcaaaatta ttcaagaaga gcatttatga
     2161 taatgtagct ttagctaata aagatgcgac gaaagatgac gttatgagag ccttaaaatt
     2221 agcaggatgc gatttaatat tagacaaatt cccagaaaga gaaaatacaa tcataggctc
     2281 aaaaggtgtt tatttatccg gtggagaaaa acaaagaatt gcaattgcta gagcaatttt
     2341 aaaggattcc aaaattatta ttatggatga agcatcagca tctattgacc cagataacga
     2401 gtttgaattg caaaaagctt tcaaaaatct tatgaaggat aaaacagtta tcatgattgc
     2461 acacaggcta tctacaatta aagaccttga tgaaattatt gttatggata gtggaaaaat
     2521 tatagaaaga gggtctgaca aagaattaat gtcaaaagat acaaggtata agagcctgca
     2581 agagatgttt aacagtgcga atgaatggag ggtttcaaat gaaagagttt tataaaaaaa
     2641 gatttgctct tacagatgga ggagcaagaa atttaagtaa agcaacactg gcttcatttt
     2701 tcgtttattg tataaacatg cttcctgcca tattacttat gatttttgct caggaagttt
     2761 tggaaaatat gggcaaaagc aatggctttt atatagtatt ctcagttttg actttgatag
     2821 caatgtatat tttgctttct atcgaatacg ataaattata taacacaacc tatcaagaaa
     2881 gtgcagattt aagaataagg acagcggaga atttatcaaa attacctcta tcttactttt
     2941 ctaaacatga catttccgac atttcacaaa caatcatggc tgatattgaa ggcatagagc
     3001 atgcaatgag ccactcaata ccaaaggtgg gcggcatggt actgtttttc ccattaatat
     3061 ctgtaatgat gctagcgggc aatgtcaaga tgggtttagc tgtaattatc ccatctattt
     3121 taagctttat atttatacct ttatctaaaa aatatcaggt taatggacag aatagatatt
     3181 atgatgtctt aagaaaaaac tcagaaagct ttcaagaaaa tatcgaaatg caaatggaga
     3241 ttaaagcata taatttatcg aaggatatta aagatgactt atataaaaaa atggaagata
     3301 gtgagagagt acacttaaag gcggaagtaa ctacaatttt aactttgtct atatcttcaa
     3361 tatttagctt tatatctctt gctgttgtga tatttgtcgg
//
LOCUS       DAVMAF010000126         3135 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMAF010000126 DAVMAF010000000
VERSION     DAVMAF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.127"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            353..1639
                     /locus_tag="VYO97_002022"
     CDS             353..1639
                     /locus_tag="VYO97_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1754761.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1654..1965
                     /locus_tag="VYO97_002023"
     CDS             1654..1965
                     /locus_tag="VYO97_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754762.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2211..2939
                     /locus_tag="VYO97_002024"
                     /pseudo
     CDS             2211..2939
                     /locus_tag="VYO97_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          984 a          543 c          675 g          933 t
ORIGIN      
        1 taaaacaaaa aatcaattct aacagtttta tatcataata ataaataaaa aagcgatatt
       61 ctagactgta tcaatccttt tccattatag gagcaacaga gtgggagtag tcatctaagg
      121 actaatttat atatttttac gagtcagtat cttgggatac tggtttttac ttttttagac
      181 tttttgacta cttgttaaaa ctggggtaat tttcgactgt ttaatagtta ttatgcaaag
      241 tctaaaagat tagaattgtc aaaacaatcc gtctaggctt gattttatcc tttatttact
      301 ataaaatgag aaggaaaaat gtcaaacttt tatattgcaa ataggagaaa ttatgacaaa
      361 aacattaaaa cgtcctgagg ttttatcacc tgcagggact ttagagaagc taaaggtagc
      421 tgttcagtat ggagcagatg ctgtctttat cggtggtcag gcctatggtc ttcgtagccg
      481 tgcgggaaac tttactttcg aacagatgga agaaggcgtg cagtttgcgg ccaagtatgg
      541 tgccaaggtc tatgtagcgg ctaatatggt tatgcacgaa ggaaatgaag ctggtgctgg
      601 tgagtggttc cgtaaactgc gtgatatcgg gattgcagca gttatcgtat ctgacccagc
      661 cttgattatg attgcagcga ctgaagcacc aggccttgaa atccaccttt ctacccaagc
      721 cagtgccact aactatgaaa cccttgagtt ctggaaagag ctaggcttga ctcgtgtcgt
      781 tttagcgcgt gaggtttcaa tggaagaatt agctgaaatc cgcaaacgta cagatgttga
      841 aattgaagcc tttgtccatg gagctatgtg tatttcatac tctggacgtt gtactctttc
      901 aaaccacatg agtatgcgtg atgccaaccg tggtggatgt tctcagtcat gccgttggaa
      961 atacgacctt tacgatatgc catttgggaa agaacgtaag agtttgcagg gtgagattcc
     1021 agaagaattt tcaatgtcag ccgttgacat gtctatgatt gaccacattc cagatatgat
     1081 tgaaaatggt gtggacagtc taaaaatcga aggacgtatg aagtctattc actacgtatc
     1141 aacagtaacc aactgctaca aggcggctgt ggatgcctat cttgaaagtt ctgaaaagtt
     1201 tgaagctatc aaacaagact tagtggacga gatgtggaag gttgcccaac gtgaattggc
     1261 aacaggtttc tactacggta taccatctga aaatgagcag ttgtttggtg ctcgccgtaa
     1321 aatccctgag tacaagtttg tcgctgaagt ggtttcttat gatgatgcgg cacaaacagc
     1381 aacaattcgt caacgaaatg tcattaacga aggggaccaa gttgagtttt atggtccagg
     1441 tttccgtcat tttgaaacct atattgaaga tttgcatgat gccaaaggca ataaaatcga
     1501 ccgcgctcca aatccaatgg aactattgac tattaaggtg cctcaacccg ttcaatcagg
     1561 agacatggtt cgtgcattaa aagaaggact catcaatctt tataaggaag atggaaccag
     1621 cgtcacagtt cgagcttaag aaaggaaaag gaaatgatag aggcacaggg tttcttagtg
     1681 gataagcaaa caagatgcat tcattaccat agcaagctgg atattattgc tttacaatgc
     1741 tatgattgta aaaagtatta tgcttgttat cggtgtcatg attcattaga aaatcaccct
     1801 tttgagccgt atcccttatc tttgatacag gataagccta ttttatgtgg tgtttgtcta
     1861 aaactactaa catataagca atataaagaa agcttaagtt gccccttttg tttttctcgc
     1921 tttaatccag gttgccaaaa tcataaggaa cgctatttta aatagcaaat catctagttt
     1981 tgaagtagga gaaaactcaa ttccaagaga aaatgaagta aatcttccca caataaaacg
     2041 cataatatca agattgttca atacctgata ctatgcgttt ttaagatttt aaagactttt
     2101 ttcctttatc tggtattttg actacttgtt aaaaccgggt taattttcaa ctgtttaaca
     2161 gttattatgc aaagtctaaa aggttagaat tgtcaaaaca atccgtctag agtatgcgtg
     2221 atgccaaccg tggtggatgt tctcagtcat gccgttggaa gtacgacctt tacgatatgc
     2281 catttgggaa agaacgtaag agtttgcagg gtgagattcc agaagaattt tcaatgtcag
     2341 ccgttgatat gtctatgatt gaccatatct cagatatgat tgaaaatggt gtggacagtc
     2401 taaaaatcga aggacgtatg gagtctattc actatgtatc aacagtaacc aactgctaca
     2461 aggcggctgt ggatgcctat cttgaaagtc ctgaaaagtt tgaagctatc aaacaagact
     2521 tggtggacga gatgtggaag gttgcccaac gtgaactggc tacaggattt tactatggta
     2581 caccatctga aaatgagcag ttgtttggtg ctcgtcgtaa aatccctgag tacaagtttg
     2641 tcgctgaagt ggtttcttat gatgatgcgg cacaaacagc aactattcgt caacgaaacg
     2701 tcattaacga aggggaccaa gttgagtttt atggtccagg tttccgtcat tttgaaacct
     2761 atattgaaga tttgcatgat gctaaaggca ataaaatcga ccgcgctcca aatccaatgg
     2821 aactattgac tattaaagtc ccacaacctg ttcaatcagg agacatggtt cgagctctta
     2881 aagaggggct tatcaatctt tataaggaag atggaaccag cgtcacagtt cgtgcttaat
     2941 gtagttgttt agttttaaaa aactatgcaa agctccatat acaacactta aacgagatta
     3001 aagaatggcg aaatcccttg atgcgcaaga gattagctgt cttttttatt ttttaagtga
     3061 taaagtcgga gtttaggcat caaagcctat caaattaaac aaagaagcga tgtcttagat
     3121 attttgaaaa aaatt
//
LOCUS       DAVMAF010000127         3132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMAF010000127 DAVMAF010000000
VERSION     DAVMAF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.128"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>3132
                     /locus_tag="VYO97_002025"
     CDS             <1..>3132
                     /locus_tag="VYO97_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1754763.1"
                     /translation="NTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELA
                     NDYFGISAMRYHIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNIT
                     INADSYFLHGLTKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLY
                     YKLKNLTYYPYNLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISA
                     DSKAGNDTQYSYEGTITKHIKQQDGIEAKIQAQIEAADKDFDQKVDKIKKDFNDQVEL
                     AKARAEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLD
                     SVARLEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQAEALSRTKNE
                     LAGASTLLAQEAKRIELDSVARLEAFKLQTTSAQTALSGDLDVLKRTIANDIRPKQAQ
                     AEAEIAKQVEALSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAE
                     ELASRIASVQVGGINLLRNTASLLIGDRSKGCWMSSSGGNGRAISVEVLAPPKKMIKN
                     MIRVIENTNGGNKDLTQLVRLRIGEKYTISCYARVASDSPNANVNLLFRSWANDTDLN
                     RKFQKSISHKNWQKYSFTFTADAIENSIQFGQSGAGIIEICAPKIESGTLATDYSEAP
                     EDIEGQISTVESNFKQRADSLEAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETD
                     TQNKLNQKLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRN
                     LWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIK
                     YENVVQGRNFWNAFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYS
                     EKTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLITEAKATF
                     ERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREESTRQATAVRELVNRDFVGKATYQ
                     EDVKGINQRIEAVK"
BASE COUNT         1000 a          587 c          775 g          770 t
ORIGIN      
        1 aacactacca cgcttgaact tatggacggt atgtttaagc tcaatcgtga gtacgtgacg
       61 gacttgcatt tcccagctga agtacgagag gttattcagg aaatctgcct gaaaacaggc
      121 attgagttag cgaatgacta tttcggaatc agcgcgatgc gttatcatat tgagcaagtt
      181 cctgagggta agaaactttc ctttagggat atgctgagcg ctatgactca gatgattggg
      241 atgtcttgtt tcttcaacag agaaggcaag atggaaatcc gtgatttgac tgagtccaat
      301 atcacgatca acgctgacag ttacttcttg catggcttga ccaagagtga gattgagtat
      361 cagatagctg gtatcacttg taagacggac aagaagtctc tgacagtcgg tatgaagaca
      421 ggtcggtctt tggaactgga caatgtcttt atgactcaga gcgctttaaa tgacttgtat
      481 tacaaactga aaaacctaac ttactatccg tataatctca actaccaagg acatttgtta
      541 cttgaggttg ggcagtgggt aaccattcag accaataaga aagagacctt taaagttcct
      601 gtgttaagtc agagctttac ttttaaaggt ggtctgagag ggcgtatcag cgcagatagt
      661 aaggctggaa acgatactca gtattcttac gagggtacga ttaccaagca tattaagcaa
      721 caagatggca ttgaagcgaa aatccaagcg cagattgaag cagcagataa agattttgac
      781 caaaaggttg acaaaatcaa aaaagacttt aacgatcaag tagaactggc caaagccaga
      841 gctgaagaag tcaagagaga actgtctgac actatcaatc agcgatttaa tagctttgac
      901 aacgggccat tgaaagaaac taagcgcaag gctgaggaag ctttgcgaaa tgctggcgca
      961 agtaccctgc ttgcacagga agctaagcgg attgggctgg attctgtcgc tagacttgaa
     1021 gcgtttaagt cgcagactac gagcgcacaa acggctctgt cgggtgactt ggacgctctg
     1081 aaacggacta tcgcgaatga tattcgaccg aagcaagcac aggctgaagc tgagattgcc
     1141 aagcaagctg aagcacttag ccggactaaa aatgaactgg ctggcgcaag taccctactt
     1201 gcacaggaag ctaagcggat tgagctggat tctgtcgcta gacttgaagc gtttaagttg
     1261 cagactacga gcgcacaaac ggctctgtca ggtgacttgg atgttctaaa acgaactatc
     1321 gcaaacgata ttcgaccgaa gcaagcacag gctgaagctg agattgccaa gcaagttgaa
     1381 gcacttagcc ggactaaaaa tgaactggct ggcgtgaagt cagcgcaagc gacgtatgag
     1441 gagacgacga ctcgtagact gtcagaactg accaacttgg ctaatggtaa agccagcaag
     1501 tcagaactca cgcagacggc tgaggagcta gctagtcgga ttgcgagtgt gcaagtcgga
     1561 ggaattaacc tcttgcgtaa tactgcgagt ttgttgattg gcgatcgttc aaaaggatgt
     1621 tggatgagtt caagcggagg aaatgggcga gcgattagcg tagaagtttt ggctcctccc
     1681 aaaaaaatga taaaaaacat gattcgtgtt attgaaaata cgaatggtgg aaataaagat
     1741 ttaactcaac ttgttagatt gcgaattggt gaaaagtaca cgatttcttg ttatgcaagg
     1801 gttgctagcg atagcccaaa tgcaaacgtg aacttgctat ttcgttcgtg ggcaaatgat
     1861 accgatttaa atcgcaaatt tcagaaatcc atctctcata aaaattggca aaaatattca
     1921 tttacattta ctgctgatgc aattgaaaat tcaattcaat ttggacaatc tggcgcagga
     1981 attatcgaaa tatgcgctcc gaaaatcgaa tcaggaacgt tagcgactga ttacagcgaa
     2041 gctcctgaag atatagaagg tcagatttca acagttgaat ccaactttaa acagcgtgct
     2101 gattcactcg aagctggtgt aagccgtctg actgaaggcc ttagaactaa agcggatatc
     2161 agctcactca acgtaactgc tgagaatatc cggcaatctg tgaagagtct tgagacagac
     2221 acgcagaaca agctaaatca gaagttgagt caggctgaat ttgaggtgcg agctggatct
     2281 atccgtcagg aaatcctgaa cgcaaccaag gataaagcag ataagacttt agttgtatct
     2341 gaagctggga aattgcgtga agaattttca aaaatgaagg tgggaggacg gaatctatgg
     2401 ataaaatcca agacggttgg agctgtaatt gaaaaattac ctgaaaacca cgtcacaggt
     2461 caaaaagaat gctataggct agagaacaac tctactttaa cgttcaacct tgaaccagat
     2521 ttcagctcaa ggttgtacca aaaagttact tttagcgctt ggatcaagta cgaaaatgta
     2581 gtccaaggtc gaaatttttg gaatgcattt aattgcttca aacattatct ttttagaaaa
     2641 aatagtgaga ccggagtaca gagtggtcca gattatgcta cgcttggtat gtataaaggt
     2701 tcggcagatt ggaaatatat tacattcact tatgactact ctgaaaaaac aaattttgat
     2761 caattgaaga catcattgcg attcaatctt gaaggtgcta caagcggtac agcttgggta
     2821 acaggaatca aggttgaaat cggtagtgtg gcgacggact ggagtcctgc gcctgaggac
     2881 gctgatggtc tcatcactga agctaaggct acctttgagc ggacagctca gggcttgcga
     2941 accgatttat cagctattca ggaatatgta aataaagacg gtcagcgaca ggaagcccta
     3001 cagcgctata ctcgtgagga gagcacgaga caagcgacag cagtccgtga gctggtcaat
     3061 cgtgatttcg ttggtaaggc tacttatcaa gaagatgtta agggtatcaa tcagaggatt
     3121 gaagctgtta aa
//
LOCUS       DAVMAF010000128         3090 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMAF010000128 DAVMAF010000000
VERSION     DAVMAF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.129"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            1..511
                     /locus_tag="VYO97_002026"
                     /pseudo
     CDS             1..511
                     /locus_tag="VYO97_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            678..878
                     /locus_tag="VYO97_002027"
     CDS             678..878
                     /locus_tag="VYO97_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754764.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATISDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            920..1324
                     /locus_tag="VYO97_002028"
     CDS             920..1324
                     /locus_tag="VYO97_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754765.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1555..2262
                     /gene="ccdA2"
                     /locus_tag="VYO97_002029"
     CDS             1555..2262
                     /gene="ccdA2"
                     /locus_tag="VYO97_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1754766.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2273..2830
                     /gene="sdbB"
                     /locus_tag="VYO97_002030"
     CDS             2273..2830
                     /gene="sdbB"
                     /locus_tag="VYO97_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1754767.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDIKAITFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
BASE COUNT         1001 a          557 c          639 g          893 t
ORIGIN      
        1 aaacaggaga gttaaaccac caagtaaaag gaacaaaacc aagaaaagtt gatagagata
       61 gacttaaaaa ctatcttact gacaatccag acgcttattt gactgaaata gcttctgaat
      121 ttggctgtca tccaactacc atcactatgc gttcaaagct atgggctaca ctcgaaaaaa
      181 agaaccacac ctactatgaa caagacccag aaaagtagcc ttatttctta agaattttaa
      241 tagtttaaag cacctagcac ctgtttagat tgatgaaaca ggattcgata cttattttta
      301 tcgagaatat ggtcgctcat taaaaggtca gttaagaaga ggcaaagtat ctggaagaag
      361 atatcagagg atttctttgg ttgcaggtct aacaaatggt gaattaatcg ctccaatgac
      421 ttacgaagag atgatgacga gcgacttttt tgaagtatgg tttcagaagt ttttcttacc
      481 aacattaacc acaccatcgg ttattatagt aaaatgaaat aagaatagga caaatcgatc
      541 aggacagtaa aatcgaattc taacaatgtt ttagaagtcc atatgtacta ttctagtttc
      601 aatataccat aactgttcct agtcaacctg caagccctgt cccagctcca agctcagctc
      661 cgactcaaga tcctagagtg gtagcaagcg cttcggctag ctcaacgagc acacaagctc
      721 aagagcaagt tgacaagtct gaacttcgtg ccttgagtca agagttagac caacgcttga
      781 aagctttggc aacaatatct gatccgaaaa tcgatgcaac caaggctgtc ctcctagatg
      841 ctcaaaaagc tccggaagat agtgccttga cagagtaagg tctgcatact gcagtagagt
      901 ctgtgaaggc tgggctgcct tggactctct caaagatgtg aaagcaaatg ctagcgacag
      961 caagcctgca caggacaaga aggatgcaaa acaaggaacg gaagatagta aggattcaga
     1021 taagatgact gaaacaaact cagttccggc aggagtgatt gtggtcagtc tacttgccct
     1081 cctaggcgtg attgccttct ggctgattcg ccgtaagaaa gagtcagaaa tccagcaatt
     1141 aagcacggaa ttgatcaagg ttctaggaca gctagatgca gaaaaagcgg ataaaaaagt
     1201 ccttgccaaa gcccaaaacc ttctccaaga aacccttgat ttcgtgaaag aagaaaatgg
     1261 ctcagcagag acagaaacta aactagtaga ggagcttaaa gcaatccttg acaaactcaa
     1321 gtaaaacatg atttatgaaa tgtcagacct aaaagacatt ttcgttcaca aaagcagcga
     1381 tgagtaatcc ttgtcgttgc tttttggcta ttttggggga taagatccgc cttgactagc
     1441 aaaagaaaag attggtccaa tagtgcccga tacgattttg cgtagcagaa agcttacaaa
     1501 taaaattgaa tatagtaaaa taggattaga attattaaga aagttggttc tttattggaa
     1561 acgatagtat ttttaatctc tgtttttcta gcaggtgttt tatccttttt ttctccttgt
     1621 atttttcctc ttctgccagt ctatgctggg attttattgg atgatcaaga aagtgcaaaa
     1681 agcttttctt tgtttgggag aaaggttctc tggtcaggct tgattcgaac actttgcttt
     1741 atcgctggta tctctctcat tttctttatt ctaggctttg gtgctggtta ctttggtcat
     1801 attctctatg caaattggtt tcgatatggc atgggagcta ttattatcat tttgggtctt
     1861 caccagatgg aaatttttca tttgaagaaa ttagaagttc aaaaaagttt tacctttaaa
     1921 aaatcagatt ctaatcgtta ttggtcagct tttttacttg gtattacctt tagctttggt
     1981 tggacacctt gtattggtcc agttttaagt tctgttttag cacttgcggc ttctggaggc
     2041 aatggcgctt ggcaaggagc gatttatact ctcatttaca ctctgggcat ggcccttcct
     2101 ttcttggtat tggcactagc ttcaggtcta gtcatgccat attttagtaa aatcaagcgt
     2161 catatgatgc tactaaagaa aattggtggt ttcctcattg ttttaatggg aattttgtta
     2221 ctattaggac aagtaaatgt tctagctgga atttttgaat aaaggaggac aaatgaaaaa
     2281 agtaatgttt gctggcttaa gtctcttgtc attagttgta ttgatggcct gtggtgagga
     2341 agaaactaaa aagactcaag cagcacaaca gccaaaacaa caaacgactg tacaacaaat
     2401 tgctgttgga aaagatgctc cagacttcac attgcaatcc atggatggca aagaagttaa
     2461 gttatctgat tttaagggta aaaaggttta cttgaagttt tgggcttcat ggtgtggtcc
     2521 atgcaagaaa agtatgccag agttgatgga actagcggcg aaaccagatc gtgatttcga
     2581 aattcttact gtcattgcac caggaattca aggtgaaaaa actgttgagc aattcccaca
     2641 atggttccag gaacaaggat ataaggatat cccagttctt tatgatatca aagcaatcac
     2701 cttccaagct tatcaaattc gaagcattcc tacagaatat ttaattgata gccaaggaaa
     2761 gattggaaag attcaatttg gtgctatcag taatgcggat gcagaagcag catttaaaga
     2821 aatgaactag aatcgataaa atctgattac aagatgtagt cagattttgg taacttctaa
     2881 aactaacttc cagtttccca caacctagct tttagtatga gaaaaacgcg tgacatcagt
     2941 ggaaatccgt cttagactaa cttccactgg ttttcttttt cttaatatat aagggttcaa
     3001 aagcgaaaag actcagaaaa agtgcacgac aaatagccct aaaacagagt catctgagac
     3061 taacttccag ttttgggaga gagatggaag
//
LOCUS       DAVMAF010000129         3070 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMAF010000129 DAVMAF010000000
VERSION     DAVMAF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.130"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(147..2648)
                     /locus_tag="VYO97_002031"
     CDS             complement(147..2648)
                     /locus_tag="VYO97_002031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1754768.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDISREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT          840 a          680 c          613 g          937 t
ORIGIN      
        1 atcatacttt cataagaagg ttcaaaaaat cctccagatt ctactctgaa ggattcctat
       61 cttatttcac aacaatgtta actaatttag ttggcttcaa tagtccagtg gactattgaa
      121 ggtggcagat agagctaata aactcgttat ttaacgacga tattaacgag tttattcggt
      181 accgcaatta ctttcacgat ttccttaccg tcaatttctg ctttgacttt ttcatcagct
      241 aaagcgattt cttgtaattc ttcacgtgat atatctttag caaccatgag tttggcacgg
      301 acttttcctt tgatttggac gacaatttca atttcatctt caaccaattt gctttcgtcc
      361 caagttggcc aagctacata agagattgac tcacctgttt ctgcgactgt ttgccagagt
      421 tcttctgcca agtgaggtgc aaatggtgca atcaattgaa taaagccttt ggcatagtca
      481 acataaagct tatcttcctt gttagcagca ttgacaaaga ccataagttg ggcaatagct
      541 gtgttgaatt tgagagactc aatttgctca gtaacagctt tgactgtttc gttgtaaacc
      601 ttgtcaagag caccattgtt ttccgcaagg atttctttac ttgtaatcaa acggtaaact
      661 cggtcaagga acttacggct tccttccaaa ccttcttctg accaagcaat cgaagcatcg
      721 agtggtccca taaacatttc ataaacacga agggtatcgg caccgtattg ttccaccaca
      781 tcgtctgggt taacaacgtt cttgagcgat ttagacatct tggctggcgc ttgctccaac
      841 tcttcccctg tttctacatg aaagaaggaa ccatcacgtt tttcaacctt gtcggttgcc
      901 acaagagcac cacggtggtc acggtagctt gttcccaaaa tcatcccttg gttaaagagt
      961 ttttggaatg gttccttagt cggaacaaca ccgaggtcat agaggaattt gtgccagaaa
     1021 cgagcgtaaa gcaagtgaag tacagcatgt tccgcaccac ccacgtagat atctactggc
     1081 aaccattgtt tgaggaggtc ctcatcagcc aatttctcag tattgtgtgg atcaatatag
     1141 cggaggtagt accaacttga accagcccat tgtggcatag tgttggtttc acgacgacct
     1201 ttgacaccat cttcacgagt cacttcaagc caatctgtca agttagctag tggactttca
     1261 ccagtacctg aaggacggat atccttggtt acaggcaaga caagcggcaa ttcagtttca
     1321 ggaacagctg ttgaagttcc atcttcccaa tgaatgattg gaattggctc accccagtaa
     1381 cgttgacggc taaagagcca gtcgcggaga cggtaggtaa ccttctcctg accacagcct
     1441 ttttcttcca accaagccac aatcttggca atagcgtctt ctttgttgag ttcatctagg
     1501 aagtctgaat tgacatgcag gccatcctct gtgtaggcag cttctttgac atctccacct
     1561 tcaagtactt cgacgattgg aaggtcaaat tgtttggcaa attcccagtc acgttggtcg
     1621 tgggcaggca cagccataac cgcacctgtt ccataactag caagaacata gtccgcaatc
     1681 cagattggca tttccttacc attgacaggg ttgatggcat aggcacctgt ccaaacacca
     1741 gttttttcct tagcaaggtc tgtacgagcc aagtcagact taaggctggc ttggtgttta
     1801 tagtctgcta cagcttctgc ttgctctgaa cttgtgatag cgtctactaa ttcatgttca
     1861 ggagccaaga cagtgaaagt cgcaccgaaa agtgtgtctg gacgagtagt aaagactgta
     1921 aattccttgt ctgttccttt tactttgaaa gttacattgg caccagttga tttaccaatc
     1981 cagttgcgtt gcatatcctt gatagactct gaccaatcta gttcatctaa gtcattgagc
     2041 aagcgctctg cgtaagccgt gattttgagc atccattggc gcattggttt gcggacaact
     2101 ggatagcctc cacgctcaga agttccgtca ggaagcactt cttcattggc aatggcagtt
     2161 cccaattcct caacccagtt tactggcact tcagcttcat aggccaagcc tttttcgtaa
     2221 agcttggtga aaatccattg agtccacttg tagtagtttg gatctgttgt gttgacttca
     2281 cgatcccagt cataagaaaa tccaagcgca ttgatttgac gtttgaagtt ggcaatgttt
     2341 tccgctgtaa attctgctgg gtcattacca gtatccatag cgtattgctc tgcaggtaaa
     2401 ccaaaagcat cccaacccat tgggtgaagg acattgtagc cttgcgcacg tttgtaacga
     2461 ctgaggatat cggttgcagt ataaccttct gggtgtccta catgcagacc agctccagac
     2521 ggataaggga acatatcaag cgcataaaac ttaggttttg atgcatctgt tcctgtctta
     2581 aatgtatgat gttctgccca gtagccctgc cacttaggct caatttcttt atgattataa
     2641 aaactcatgg tctctctcca attttgtgat gttactatta taccattttt gagggaattt
     2701 gaaatgtgtt taaagaaaaa actgtatact gtatctcatt aattttgatg actatgaatt
     2761 gctctatctc ccacttttta tcccattgtt atgcagtaaa tcaaggagta agtcaagaag
     2821 cttatctcgt atagcacaaa atacttactt tagaccgaag ataaaactgg aatgctaatt
     2881 aatgtattca caataaatag ccgacaacct atgtcgcttt ttgatttatg aagaacgtat
     2941 agtggactga atctgaaata gtacgaaaca attgctaaaa cacttataga aattaatttt
     3001 actttcccaa tcgatttgtt ctcatcttat ttcaatctgc tatagaattc aatacgagga
     3061 tcaataaaag
//
LOCUS       DAVMAF010000130         3030 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMAF010000130 DAVMAF010000000
VERSION     DAVMAF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.131"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..165
                     /locus_tag="VYO97_002032"
     CDS             <1..165
                     /locus_tag="VYO97_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1754769.1"
                     /translation="VSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTLITG
                     KNGTGKSTLL"
     gene            complement(531..1841)
                     /locus_tag="VYO97_002033"
     CDS             complement(531..1841)
                     /locus_tag="VYO97_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1754770.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGKTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2078..2950
                     /locus_tag="VYO97_002034"
     CDS             2078..2950
                     /locus_tag="VYO97_002034"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1754771.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          921 a          509 c          696 g          904 t
ORIGIN      
        1 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
       61 gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc aggcgtgaca
      121 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat tgaaactaga
      181 atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc tgatctattt
      241 gtcctgttct tatttcattt cactacatct caaattgagt atgacgaagt gcgctcccat
      301 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
      361 tgggatgaat ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag
      421 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
      481 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag
      541 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca
      601 cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt
      661 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
      721 gtggctgttg tgagtaagtg aatatcaatc tggggatttt gagcactgat gatagcaatg
      781 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcactccg
      841 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggatgttgt catgaggtgc
      901 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
      961 agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt ccctaatttg
     1021 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgttttttga
     1081 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
     1141 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
     1201 ggttggtcaa tagtcaagga tgagtttgtt tttcccttac tatagtgact gtaacaggat
     1261 aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt
     1321 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
     1381 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc
     1441 aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata
     1501 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
     1561 tagagggcgt tagggtaggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
     1621 gtaccgttag ttattgcaaa gttataaaag agagagacgc taaaaatggt aaaaaagagt
     1681 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
     1741 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
     1801 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
     1861 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac
     1921 ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga
     1981 attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagtg attgcctcta
     2041 atgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt caactgattt
     2101 aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga
     2161 agattacagt accaatgcca tttttgatca tcatggccaa gctaaggtgt cgctttttgc
     2221 caaggaagct ggtgttttag cggggctaac cgtttttcaa agggttttta ccctatttga
     2281 tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg
     2341 cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc
     2401 cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc
     2461 cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct
     2521 ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta atttatcaga
     2581 tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc
     2641 tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc
     2701 tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt
     2761 ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg aatgttctgg
     2821 aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag
     2881 tggtagttta acccatagtg ctaagagtct tgatttttcc atgaaggggt taacctacct
     2941 tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc tagaacgagt
     3001 tttatgtcag atagtttaaa cgcctcttca
//
LOCUS       DAVMAF010000131         3019 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMAF010000131 DAVMAF010000000
VERSION     DAVMAF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.132"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            119..1600
                     /locus_tag="VYO97_002035"
     CDS             119..1600
                     /locus_tag="VYO97_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1754772.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKEISHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1961..2962
                     /locus_tag="VYO97_002036"
     CDS             1961..2962
                     /locus_tag="VYO97_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1754773.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
BASE COUNT         1076 a          410 c          521 g         1012 t
ORIGIN      
        1 gtgttattat acgaaataaa agattttagg aaaagtcgtt atatcatgct atacctattc
       61 tttgtggtat aattgcaaga ggtttaatcc gataatttat aaagagagaa agatattcat
      121 gagagattta ttatctaaaa aaagtcatag gcaattagaa ttattagaat tattatttga
      181 acataaacgt tggtttcatc gttctgaact agcagagtta ttaaattgta cagaacgtgc
      241 agtcaaagat gatctatccc atgttaaatc tgcttttcct gacttgattt ttcattcttc
      301 tactaatggt atacgcatta ttaataccga tgatagtgat attgaaatgg tttaccatca
      361 tttctttaaa cattcaactc atttttcgat tttagaattc atcttcttta atgaaggttg
      421 tcaagctgag agtatttgta aagaatttta tatcagttca tcttcgctct atcgtattat
      481 tagccaaatc aataaagtga ttaaaaggca atttcaattt gaagtcagtc tgacccctgt
      541 tcaaatcatt ggaaatgaga gagacattcg ttactttttt gcacaatatt tttcagaaaa
      601 atattatttc ctagaatggc catttgaaaa tttttcatca gagccactat ctcaattgtt
      661 agaattggtt tataaggaaa caagctttcc aatgaatttg tcaacgcata gaatgctaaa
      721 gttgctccta gttacgaacc tatatagaat aaagtttggt cattttatgg aagtagataa
      781 agattctttt aacgaccaaa gtttggattt tttaatgcag gcagaaggaa tagaaggtgt
      841 tgctcagagt tttgaatcag aatacaatat ctctttagat gaagaagttg tttgccaatt
      901 atttgtgtct tactttcaaa aaatgttttt catagatgaa agtctcttta tgaaatgcgt
      961 aaaaaaggat agttatgttg aaaaatctta ccatctattg agtgatttta ttgatcagat
     1021 ttcagtcaag tatcagattg aaatggagaa taaggataat ctgatttggc atctgcataa
     1081 taccgcacat ctgtatcgtc aagagttgtt tactgagttt attttatttg atcaaaaagg
     1141 gaatacaatc aggaactttc aaaatatttt tccaaaattt gtttcagata taaaaaaaga
     1201 gatttctcac tatttagaga ctcttgaggt ttgttctagt tcaatgatgg taaatcatct
     1261 atcttatacc ttcatcactc ataccaaaca tttggtgatt aatttgctac aaaatcagcc
     1321 taaactaaag gttttggtta tgagtaattt tgatcagtat catgcaaaat tcgttgcaga
     1381 gacactttct tattactgta gcaataattt tgaactggaa gtttggaccg aattagaatt
     1441 atcaaaggaa tctttagaag attcacctta tgatatcatt atttccaatt ttattattcc
     1501 tcctattgaa aataagagac tcatctattc aaataatata aacacggtct cactcatata
     1561 tttgttaaat gccatgatgt ttattcgatt agatgagtaa ccaaaaataa caaaaaaacg
     1621 ctatttcgcg tttgaactgt acctcaaaag ttagacagaa aaaatctaat ttttggggtg
     1681 tttttgttaa gaaaagacct tcaatcattt aagcaaaatg tacatctaga tttatagccc
     1741 tctaaaactc ttgttttttt gtagaagata cgaaaaaagc tatgaaacca aggcttttcc
     1801 tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga
     1861 ttttattcat gatgggataa atgatctggc caaaaatgac tgagaaatag ttgcttaaaa
     1921 agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc
     1981 aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt
     2041 aattaataaa gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac aagcgattaa
     2101 aactttgaat tacattccag attactacgc tagaggaatg aaaaaaaatc gaacagaaac
     2161 gattgcaatc attgtaccaa gtatctggca tcccttcttt tcagaatttg ctatgcatgt
     2221 ggaaaatgaa gtctataaga gaaataacaa attactctta tgttctatca atggtacaaa
     2281 tagagagcaa gactatctgg agatgttgcg tcataataaa gttgatggag tggttgccat
     2341 tacctatagt ccaattgaac attacttgac gtcaggaatt ccctttgtta gtattgaccg
     2401 cacatactca gatattgcca ttccttgtgt ttcatcagat aatgatgcag gtggaagaga
     2461 agcagctaaa caattaatta gtaaaggatg tcagcattta gcctttgtgg gcggacataa
     2521 tacaaccatt aatgaaacca aacgtcgtag aatttcattt gaaaagtatg tccaagagca
     2581 taaaatacct cttagtattt ttgatttgga tgagacagtt actgactatt atggaaagct
     2641 agagagtttc ttggaagaaa attcttctat agatggaatt tttacaatca acgattttac
     2701 agctttggat gtgattgaaa tactagaaaa aaatggtaaa cgtattcctc atgatgtcca
     2761 aattattgga tacgatggaa ttaaaatggc tggagataga gattatctac tttcaactat
     2821 tgaacaacca ttggaagaaa tggcaaaaga agcggttcgt attttgtttg atataattga
     2881 tggaaagact gttaatttgc agacaatcct accagtaaaa tttgttgaag gaaaaacaac
     2941 aaaaaatgaa aataagtctt gacagaaagc gctatcaatg atagaatgaa ttcagataaa
     3001 aaagatttat ttttaaaac
//
LOCUS       DAVMAF010000132         3013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMAF010000132 DAVMAF010000000
VERSION     DAVMAF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.133"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(101..2779)
                     /locus_tag="VYO97_002037"
     CDS             complement(101..2779)
                     /locus_tag="VYO97_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1754774.1"
                     /translation="MAVFVSLDGIVVEVLDVFSSFNGDSEFFLCKRLKDKSQFVMERS
                     QFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKSFINDQGKIQYFPSYDCGWKQLL
                     PEKRSFQTLTDSVLKSHFRGETAIGIFPMHLDDSCHFLVLDFDEGDWKEAGLTIRRIA
                     RERQMEAHLEISRSGHGLHIWFFFEEAIPSREARLFGKKLIELAMQESMQLSFDSFDR
                     MFPNQDVLPKGGFGNLIALPFQGEAYHQVRTVFVDEQFQPYEDQWRYLQEIQRVSTAK
                     VALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFFLKNMASFSNPEFYLKQAM
                     RQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSVEDRRKVQRSISVEFKGEL
                     TFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKTKTIILVHNKQLLDQWLDR
                     LNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSL
                     LDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILH
                     TADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQV
                     AEGRNILVLVNRIQQIDVFEKLLKEKEVDDCYIISGKTKVRERTSLLETLEQLDKGFV
                     LLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIH
                     VPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKVLREDLAGERQECLLILPY
                     VHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKIKVSFTQSKIVTPILLVNK
                     TIVWYGAMPLLGKVDEMTILRLESASIASELVAGLR"
BASE COUNT          962 a          697 c          456 g          898 t
ORIGIN      
        1 acttatagaa atttttttat tttgtgtgct tcgaatgtga caatggcaac agtctcacta
       61 aaaaacgaaa gtcatacata gaaattttta aaaattttct ctatcgtaaa cctgccacta
      121 gttcagaagc tatactagct gattccaaac gtagtatggt catttcatct accttcccta
      181 ataatggcat tgcaccatac caaacaatag tcttattcac caaaagaata ggcgttacta
      241 tttttgattg agtaaaagac actttaattt tctggctctt caactggtct agccatgctt
      301 tatttgcaac cgtctctggt atacatatct caatttgact aatcctaaat tcttttagaa
      361 aattcatcag ttttgtctgg tgcacataag gtaaaataag cagacattcc tgtctttccc
      421 ctgctaagtc ctctctcaac accttctcat atctactatc aacataaacg aattgtttct
      481 cctcaccctc gatgacacga taatccatct ttcgataagc tacttgtcgt ttctgaaaca
      541 tcttttctaa ataaggaaca tgaatatcca cataatcgaa aatacgcacc aaagacttat
      601 ccttgtagtt tctatgaatc cgacctgcat actgaattaa attatttttc caagaaaagg
      661 gtgctgccaa gataagcgtg tccaactgag gtaagtcaaa accttcgcca atgtattttc
      721 cagtagacaa caaaacaaac cctttatcta actgttctaa cgtctccagt aaactcgttc
      781 tttctcggac tttggttttt ccgctaataa tgtaacagtc atcaacctct ttctctttca
      841 ataacttttc aaagacatct atctgttgaa ttcgattaac taaaaccaag atattccgtc
      901 cttctgccac ttgggctaga atatccttga gaatcaactg attcctcgct gagtcagtag
      961 caatccaatc actaagctgt ataaaattac ttgctttggt cttttcaatc tccaaatgac
     1021 caaaagaagt gaatcttaat tgcaattgcc gtttaaaatc cgtttccctc ttatcagcag
     1081 tatggagtat ctcaccaatt ctctgaaaaa caataggctc atgaccattc ttacgctcag
     1141 gcgtagccgt caaaccgtaa agatacttcc ctctaaactg agcaacaact ttttcaaaca
     1201 tcaaggcaga gacatgatga cactcatcca caatcatcat ctcatactca tccaaaagac
     1261 tttgactatt ttccaactta aatagagatt gaatcataac gacatcaacc agtttactca
     1321 gccatttctt agtcccaccg tactgtccaa catagccgat taccttttca cgacctgatg
     1381 ccgtataacg gatagcctcc tcctcttcga aagtcaaaaa gcagtttaag cgatctagcc
     1441 attggtctaa gagttgctta ttatggacta gaataattgt ttttgttttc cgttcagaga
     1501 taagagcagc acctaaaacg gtcttcccaa aaccagtctc cgcatgaagt aaaccatttt
     1561 ctttagaagt catatctgac agggctaact cttgctcaaa agtgagttct cccttaaatt
     1621 ccacgctaat agacctttgt acctttctcc tatcttccac agatacctgc ttaaatttat
     1681 cttgcaatgg atacagcaaa cctcttggca accataaata atgatcggat tctccaaata
     1741 aatacattct ctcaggaatt tgataggttg gctgtcgcat agcctgcttt aaataaaatt
     1801 cgggattaga aaatgaagcc atatttttca agaaaaacag tgtcttgggt gtcacagacg
     1861 atttttcaag ttggatcata ttggataaga cgatcttcaa ctccttgtcc aattcttgct
     1921 tgcctaactc ttcttggatt aacagtgcca ctttagcagt tgaaaccctc tgaatttctt
     1981 gtagatacct ccattggtct tcataaggct gaaactgttc atccacaaag accgttcgca
     2041 cttgatggta agcttctcct tgaaaaggca aggcaatcaa atttccaaat ccccccttag
     2101 gaaggacatc ctgatttgga aacatgcgat caaaagaatc aaaggacagt tgcatacttt
     2161 cctgcattgc cagttctatc agtttctttc caaacaagcg agcctctcga ctcggaatcg
     2221 cttcctcaaa gaagaaccaa atatggagtc cgtgacccga acgagaaatc tctaaatggg
     2281 cttccatctg gcgttccctg gctattcttc gaatggttaa accagcttct ttccaatctc
     2341 cttcatcaaa atccagtacc aaaaaatgac agctatcatc taagtgcata ggaaagatac
     2401 cgatagctgt ctccccacga aaatgagatt tcaaaacgga atccgtcaat gtctggaaac
     2461 tccgtttttc aggtagcaac tgcttccaac cacaatcata ggaaggaaaa tactgaattt
     2521 tcccttgatc attgataaaa ctcttagcat aaacatcata acggccagca aacacagagg
     2581 taaacaattg taatttttct tgcgtcgtca agcgactact ttgaagttgg aacatctcct
     2641 cgaactgaga gcgttccata acaaactgac tcttgtcttt aagacgttta cacaagaaaa
     2701 actcactatc cccattaaaa gaagaaaaga catcaagtac ttctaccaca attccatcca
     2761 aagacacaaa aacagccata agagtcacct ccttgattcc tataggctga ttataacaag
     2821 aatagctgaa attgtacacg aaaataaaat cctaatagta ctcattttgt atgtggctaa
     2881 tattccgtct cgctccggaa tgtacgaggt aaatagagtt taaacgagca gtctataaaa
     2941 ttcaaaaaac gcatcatatc aggtgtacat gaactacacc ccaaaagtta gacagaaaaa
     3001 atctaacttt tgg
//
LOCUS       DAVMAF010000133         2987 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMAF010000133 DAVMAF010000000
VERSION     DAVMAF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.134"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>2987
                     /locus_tag="VYO97_002038"
     CDS             <1..>2987
                     /locus_tag="VYO97_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1754775.1"
                     /translation="NTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELA
                     NDYFGISAMRYHIEQVPEGKKLSFRDMLSAMTQVIGMSCFFNREGKMEIRDLTESNIT
                     INADSYFLHGLTKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLY
                     YKLKNLTYYPYNLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISA
                     DSKAGNDTQYSYEGTITKQIKQQDGIEAKIQAQIEAADAAFDAEFDKREKAITDAIEL
                     AKARAEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLD
                     SVARLEAFKSQTTSAQTALSGDLDVLKQTIANDIRPKQAQAEAEIAKQAEALSRTKNE
                     LAGASTLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQ
                     AEAEIAKQVEVLSRTKNELAGVKSAQATYKETTTRRLSELTNLANGKASKSELTQTAE
                     ELSSKIASVQASGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGHKALKIIGL
                     NPSGRDGGNPKITYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYK
                     TGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSY
                     SEAPEDIEGQISTVESTFKQRANSLDAGVSRLTEGLRTKVDISALNVTAKNIRQSVKS
                     LETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELSSKIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTAAIDSESKYLGHKALKIIGLNPSGRDGGNPK
                     VTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESNFKQRADSLEAGVSRLTEG"
BASE COUNT          965 a          589 c          721 g          712 t
ORIGIN      
        1 aacacaacca cacttgaact tatggacggt atgtttaagc tcaatcgtga gtacgtgacg
       61 gatttgcatt tcccagctga agtacgagag gttattcagg aaatctgcct gaaaacaggc
      121 attgagttag cgaatgacta tttcggaatc agcgcgatgc gttatcatat tgagcaagtt
      181 cctgagggca agaaactttc ctttagggat atgctgagcg ctatgactca ggtgattggg
      241 atgtcttgtt tcttcaacag agaaggcaag atggaaatcc gtgatttgac tgagtccaat
      301 atcacgatca acgctgacag ttatttcttg catggcttga ccaagagtga gattgagtat
      361 cagatagctg gtatcacttg taagacggac aagaagtctc tgacggtcgg tatgaagaca
      421 ggtcggtctt tggaactgga caatgtcttc atgacccaga gcgctttaaa tgacctgtat
      481 tacaaactga aaaacctaac ttactatccg tataatctca actaccaagg gcatttactg
      541 cttgaggttg ggcagtgggt aaccattcag accaacaaga aagaaacctt taaagttcct
      601 gtcttaagtc agagctttac tttcaaaggt ggtctaagag gtcgtatcag cgcagatagt
      661 aaagctggaa atgatacgca gtattcttac gagggtacga ttaccaagca gattaagcaa
      721 caagatggca ttgaagccaa aatccaagcg cagatagaag cagcagacgc agcctttgac
      781 gctgaatttg acaaacgtga gaaagctatc acggatgcca tcgaacttgc caaagccaga
      841 gctgaagaag tcaagagaga actgtctgac actatcaatc agcgctttaa tagctttgac
      901 aacgggccat tgaaagaaac taagcgcaag gctgaggaag ctttgcgaaa tgctggcgca
      961 agtaccctgc ttgcacagga agctaagcgg attgggctgg attctgtcgc tagacttgaa
     1021 gcgtttaagt cgcagactac gagcgcacaa acggctctgt cgggtgactt ggatgttcta
     1081 aaacaaacta tcgcaaacga tattcgaccg aagcaagcac aggctgaagc tgagattgcc
     1141 aagcaagctg aagcacttag ccggactaaa aatgaactgg ctggcgcaag taccctactt
     1201 gcacaggaag ctaagcggat tgagctggat tctgttgcta gacttgaagc gtttaagtcg
     1261 cagactacga gcgcacaaac ggctctgtcg ggtgacttgg atgccctgaa acggactatc
     1321 gcgaatgata ttcgaccgaa gcaagcacag gctgaagctg agattgccaa gcaagttgaa
     1381 gtacttagcc ggactaagaa tgaactggct ggcgtgaagt cagcgcaagc gacgtataag
     1441 gagacgacga ctcgtagact gtcagaactg accaacttgg ccaatggtaa agccagcaag
     1501 tcagaactta cgcagacagc tgaggagctc tctagtaaga tagcgagtgt gcaggcatcc
     1561 ggtcgaaatc tattcttgaa ctcactattc aagcaggata tttcaaaaac aggaatttgg
     1621 acaacgagta catatacggc tactatcgat agcgaaagta agtatcttgg acacaaggct
     1681 cttaaaatta taggtctgaa tccatctggc cgtgatggag ggaatcccaa gattacttat
     1741 ccagctctgg gtcaattcgg gaaagtaatt cccggaagta cgactaatca agatgtaacc
     1801 attagttttt atgctaaggc aaataaaaat ggaataatgc taagatctcg attagggaat
     1861 atcggatata aaactggaaa tgtgacattg tcgacagaaa ttaaacgata tgttgtccat
     1921 attccaaaag gttggacaaa cgaatccaag cagaccacaa atgaatggtt gttcaatttc
     1981 aaccaggaag gaaccgtttg gatttggatg ccgaagtttg aaataagcga tgtagatact
     2041 tcttattcag aagctcctga agatatagaa ggtcagattt caacagttga atcgaccttc
     2101 aaacaacgag ccaactcact cgacgctggt gtaagccgtc tgactgaagg tcttagaact
     2161 aaagtggata tcagcgcact caacgtgact gctaaaaata ttaggcagtc ggtgaagagc
     2221 cttgagacag acacgcagaa caagctaaat cagaagttga gtcaggctga atttgaggtg
     2281 cgagccggct ctatacgtca ggaaatcctg aacgcaacca aagataaagc cagcaagtca
     2341 gaactcacgc agacagctga ggagctctct agtaagatag cgagtgtgca ggcatccggt
     2401 cgaaatctat tcttgaactc actattcaag caggatattt caaaaacagg aatttggaca
     2461 acgagtacat atacggctgc tatcgatagc gaaagtaagt atcttggaca caaggctctt
     2521 aaaattatag gtctgaatcc atctggccgt gatggaggta atcccaaggt tacttatcca
     2581 gctctgggtc aattcgggaa agtaattccc ggaagtacga ctaatcaaga tgtaaccatt
     2641 agtttttatg ctaaggcaaa taaaaatgga ataatgctaa gatctcgatt agggaatatc
     2701 ggatataaaa ctggaaatgt gacattgtcg acagaaatta agcgatatgt tgtccatatt
     2761 ccaaaaggtt ggacaaacga atccaagcag accacaaatg aatggttgtt caatttcaac
     2821 caggaaggaa ccatttggat ttggatgccg aagtttgaaa taagcgatgt agatacttct
     2881 tattcagaag ctcctgaaga tatagaaggt cagatttcaa cagttgaatc caactttaaa
     2941 cagcgtgctg attcactcga agctggtgta agccgtctga ctgaagg
//
LOCUS       DAVMAF010000134         2914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMAF010000134 DAVMAF010000000
VERSION     DAVMAF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.135"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VYO97_002039"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VYO97_002039"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1754776.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKEPQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1400..2176
                     /gene="recX"
                     /locus_tag="VYO97_002040"
     CDS             1400..2176
                     /gene="recX"
                     /locus_tag="VYO97_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1754777.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2265..2798
                     /locus_tag="VYO97_002041"
     CDS             2265..2798
                     /locus_tag="VYO97_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1754778.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          932 a          533 c          530 g          919 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaacaatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggtcttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctcgag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtgataa gtaagtaatt ctgctaagcg
      841 attagcaatc acttggggtt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atacccctat cttatcacat
     1381 tttgagttat aatagagcta tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct
     1441 gatggagctt gataatggcg acaaatgcta tatcaccgaa gatacaattg ttcgttttat
     1501 gttatcgaga gataaggtga taagcgaaga ggaattgaaa gagattcaag actttgctca
     1561 attttcttat ggtaagaatc tggccctcta ccacttatcc tttaaagcac gcactgaaaa
     1621 agaagtcaga gaatatctga aaaaatacga tattgatgaa aacatcgttt ctcaagttat
     1681 tgctaatctt aaagaagata agtggattaa tgatggtcag tacgcttatg ctatcatcaa
     1741 taccaatcaa ctttcaggag acaagggtcc ttatgtgctg actcagaaac tagctcaaaa
     1801 agggatttca aaatctacta tagaagagaa cttgaaagaa tttgattttt ctgaagttgc
     1861 tcaacgtgta gctaataaac tattgaaaaa atatgaggga aaacttccag ctcgtgccct
     1921 gcaagataag attatccaga acttgactaa caagggcttc tcttattctg atgctaaaat
     1981 tgcctttgac gacttagata gccaagttga ccaagaaacg actcaagaac tcatcttcaa
     2041 ggaacttgat aagcaatata ctaagtatgc tcgaaagtat gaaggatacg aactgaagca
     2101 gcgtttaact caagttttag cacgaaaggg ctacgatttt tcggatatag caagcgctct
     2161 tagagaatat ctttaatatt tccaagtgaa attcacagat tttaggtaat tttatggtac
     2221 aatagtaaat gataaactta taaattgtag aaagttggtt agttatgaag cttccaaaag
     2281 aaggcgactt tattacaatt caaagttata agcatgatgg gagtctccac cgaacttggc
     2341 gggacaccat ggtactaaaa acaacagaaa acgccattat tggtgtcaac gatcatacac
     2401 ttgttaccga aagtgatggt cgtcgttggg tcactcgaga accggctatt gtttactttc
     2461 acaagaaata ttggtttaat atcattgcca tgattcgtga taatggaact tcttactatt
     2521 gcaatatggc tagcccctac tatctggatg aagaagcact gaagtatatt gattacgatt
     2581 tggatgttaa aatttttaca gatggggaaa aacgtctctt ggacgttgaa gagtacgagc
     2641 gtcacaaacg caaaatgaat tattctgatg acttggatta tattttaaaa gaacatgtca
     2701 aaattcttgt tgattggatt aacaatggac gtggtccttt ctcagaagcc tatgtaaaca
     2761 tttggtacaa gcgctatgta gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa
     2821 aatcctgacc tttttttggt agtataaaat aaattgtgta aacacaaaaa ggaataaatc
     2881 tattatagca gagttacaac acattactag aaag
//
LOCUS       DAVMAF010000135         2901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMAF010000135 DAVMAF010000000
VERSION     DAVMAF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.136"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(14..328)
                     /locus_tag="VYO97_002042"
     CDS             complement(14..328)
                     /locus_tag="VYO97_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1754779.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(458..697)
                     /locus_tag="VYO97_002043"
     CDS             complement(458..697)
                     /locus_tag="VYO97_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV1754780.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(749..2185)
                     /locus_tag="VYO97_002044"
     CDS             complement(749..2185)
                     /locus_tag="VYO97_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1754781.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFAAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVTADGMVHD
                     DYRIEYLDAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(2375..2713)
                     /locus_tag="VYO97_002045"
     CDS             complement(2375..2713)
                     /locus_tag="VYO97_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1754782.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          962 a          604 c          363 g          972 t
ORIGIN      
        1 tttcatgtgt tcatcaaata ttctcaagaa tcaaattagc cattttttca attcccattg
       61 gaataggaat ataggcttga ggaggtattt gtacaactgg ttttcctgct ttagaaccag
      121 cctcttcaaa ttgcttaaag tacatttttg tttgaggact gacaagatac aaatcaaaag
      181 ctgctgctgc gatagctttc cctccttcag tagcactaat agcatcaact acaatatctt
      241 tcccttttcc ttttagaaac tctgttgttt tctgtgccat aagtgatgaa gacattcctg
      301 ctgcacaaat aattaaagct tttgccataa tattttctcc ttttcttaaa tccaatcaaa
      361 gctgtgctaa gttggcttat ttgttatcta tttttattat aaaataaagc gtttccaatg
      421 acaattccct cattttccta aatgatatgg aaaaaaatta tttatacttc aatttataaa
      481 ataaaattat tcctgagagt agaaatgaaa cactatttgc taaaatcaaa ggcaagtctc
      541 ctatacgaat accatgagca agccacaatg caataccaat aacttgcata acatacatac
      601 ctagagcaat agagcctgtg tcctttgtct taactacacg aaaaacttgt ggtaaaaatg
      661 caaatgttgt taaaattgct gcaatacttc caatcatatg tcacctcaat atgctaaaca
      721 aactgagaat aatctcagtt tgtttatact attctactga ttcaccgtta gatgaaataa
      781 cttccttata ccagccaaaa gattttttcg gggaacgatt ataacttccc ttcccattat
      841 catctttatc tacataaata aagccataac gtttccgcat ttcaccggta ccagctgaaa
      901 ccaaatcaat acatccccat ggagtataac ccattaaatc aacaccatct tcaactacag
      961 cctttttcat ttcacgaata tgggcatcta gatattcaat tctataatca tcatgtacca
     1021 taccatctgc tgtaacttga tctatagctc caaaaccatt ttcaacaata aagagtggta
     1081 agtgatagtg gtctgtaaac caatttaacg cataacgcaa accttctgga tcaatttgcc
     1141 actcccattc agaagcctta acataattat ttttcactaa atcttctgtt tcaagataat
     1201 caaaataagg attattttca cgatgagagt cgatagcaaa ggacatatag taactgaaac
     1261 caatgtaatc tacagtccca ccaagtaaat cttctttatc ctgggcagca aaatcaactg
     1321 aaataccttt tcgttcccaa tacttgaaaa tatgctcagg atatttacct aaaacatgca
     1381 catcagcaaa ataataacgc ttctgcatag ctttcattgc cattaagata tccttaggat
     1441 tgcaagtagc tggataaatt ggacacatcg caatcataca acctatttga aaatctggat
     1501 taatctcatg accaattttt acagctcgtg cagaagcaac taattcgtaa tgtgctgctt
     1561 gatacataat tgcttctcta ttatcacctt cctcatatac aatacctgag ttagtaaatg
     1621 gtgcaaaatc ttcctgataa ttcgcttgat tattgatttc attgaaagtc atccaatatt
     1681 taaccttatc tttgtaacgt ttaaatacga cttctgcaaa acgagcaaag aaatcaatca
     1741 atttcctatt tttccaacca ccatattcgg tcactaagtg ataaggcatt tcaaaatgag
     1801 atagagtgat gacaggttca ataccattct ttaagcattc atcaaaaaga ttatcataaa
     1861 actgtaatcc ttcttcattc ggctctaact catcaccttt tggaaagata cgcgtccatg
     1921 caatagaggt acggaagcac ttgaatccca tttcagcaaa aagtgctatg tcttctttat
     1981 aacgatgata aaaatctatc gcctcatgat ttggataata tttaccctct aaaactcccg
     2041 aagtaatttc acgagctact ccatgacgac cagcagtcat aacatcagca acactaattc
     2101 ctttgccacc ttcttgccat ccgccttcaa gttgatgagc agcaacagca ccaccccata
     2161 aaaatctatc tttaaaagta gtcatctttt ttcctcctga ctttgatact cttattataa
     2221 accttaaacc aaaatatgaa aacgcattct ttttccttat tgttaaggaa agaagtaatt
     2281 tttaatggaa atagaacaat atcttcttgt attctcgtaa tgatatcttt acgattttca
     2341 atactttcaa actacaaaaa ctctcacaat aattctaatt ccctgtgtct ataaacgact
     2401 tatcgctttc tggcatccca gaatcatctt ctatataacg ttcaacttgc atctgcaagt
     2461 gatatttttt tcttaaatct aagattttct gcattgtctt tgattgatga tgttcatcta
     2521 aagcttcttg atttatccac tgatcaataa ggagaatagt tccctctttt tcaattggta
     2581 aaaaatattc gtatttcaag ttaccttttt gatttctaat ttcttcaaca aggccactat
     2641 caagcatttc tcttgcaaac tttattgcac tatctccatc acctttataa tatacatgaa
     2701 tagtcaatgt catcttatat cctccaaaat catccttcaa ttttaaaaaa acaagtttag
     2761 atgaggatct aaacttgttt tttatgaact aattatctaa cgtttcgcca ttactttcaa
     2821 tcacttcttt ataccaataa aatgattttt tcttatagcg atttatagtc aattgaaaca
     2881 agaacaagac aaaagagcct c
//
LOCUS       DAVMAF010000136         2594 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMAF010000136 DAVMAF010000000
VERSION     DAVMAF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.137"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(146..244)
                     /locus_tag="VYO97_002046"
                     /pseudo
     CDS             complement(146..244)
                     /locus_tag="VYO97_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(270..902)
                     /locus_tag="VYO97_002047"
     CDS             complement(270..902)
                     /locus_tag="VYO97_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1754783.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLVPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(924..1796)
                     /gene="sdaAA"
                     /locus_tag="VYO97_002048"
     CDS             complement(924..1796)
                     /gene="sdaAA"
                     /locus_tag="VYO97_002048"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1754784.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1805..2476)
                     /gene="sdaAB"
                     /locus_tag="VYO97_002049"
     CDS             complement(1805..2476)
                     /gene="sdaAB"
                     /locus_tag="VYO97_002049"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1754785.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
BASE COUNT          714 a          548 c          572 g          760 t
ORIGIN      
        1 cttggtaata tgcgttttat tatgtagaga cttatactct tcgaaaatct cttcaaatca
       61 cgtcaacgtc gccttgccgt atatggttac tgacttcgtc agttctatcc acaacctcaa
      121 aacagtgttt tgagctgact tcgtcagttc catccacaac cttaaaacag tgttttgagc
      181 agcccgcagc tagtttccta gtttgctctt tgattttcat tgagtattac ttcattttct
      241 tctgaaatgg aattgttacc cagtctatgc tattgaaaat acgccaaaac ctctaagggt
      301 ttgtgagcga tataatcagg ttgatagttt agtagatctg cttgctctcc aaatccccaa
      361 gtgatggcca atttctgaat acctgtttct cgagctccca gcatatcaaa cttggtatct
      421 ccgatgatga tggcttgttc tggtactagt tgatgtgtct gcaaggcttg gtgaatgaca
      481 tctgccttat ggggtgcttc agggctagaa ccataaatgc catcaaagaa atgatggatt
      541 tccaagtttt ttgccatgtc ttgagcagta gatgtattct ttgtcgtggt gatgtagagt
      601 ggataactgc tcgataactc ctcaagcaag tctataatct gaggaaagag ttgagcttca
      661 tagatgcctt ttgccttata gtaagaacga tatatctgca cggcttcaga aatttggtct
      721 ttggacaggc aggtcgcaaa actactttcg agaggtggtc ccataaaacc acgaatagtt
      781 ttggcatcag ggctaggcac ccccagctct ttaaaggtat aggtaaaggc attgtgaatc
      841 ccgatagaac tatcaacgag ggttccatcc aaatcgaaaa aaatcgctgt gatagaggtc
      901 atggtttctc ctatttgata agcttattct ccgaaaattt ctttttggag gcgacgacca
      961 gtaggggtgg tagcgagtcc accttcagct gtttcacgaa aggcagttgg catgcttgct
     1021 cctacttggt acatggcatc gatcacttca tccacaggga ttttagattc gatacctgcc
     1081 aaggccatgt ctgctgcgat gaaagcaaag ctagctccca tggcattacg tttgacacag
     1141 ggaacttcga ccaaacctgc aacagggtca cagatgaggc ctagcatatt tttaatgaca
     1201 aaggcaatag cttgactggc ctgataaggt gttccacctg cagccagagt caaggcggca
     1261 gcactcatag cagaggctga accaacttca gcttgacacc caccctcagc acctgagatg
     1321 gaggcattgt ttgcgatgac tagtccaaag gcaccagcag caaagaggaa atccaattgt
     1381 tgctcgtggc tgaggtctaa tttttcaata gcagcagtga gaacggaagg gagacaaccg
     1441 gcacttcctg cggtaggagt agcgcagacc aagcccattt tagcattgtg ttcattgact
     1501 gcgatagcat ttcgggcagc agagagaatc gtataatctg acagagtttt tccgttttcg
     1561 atgtagtgat ccaatttggc agcatctcca cctgtcaggc cactacgaga tttattttca
     1621 ttgaggccaa gttggacaga ggctttcata acttccagat tgcgttccat gagaaggaag
     1681 acttcttcac gttcgcgacc ggtcaattca aactctgttg taatcatgag ttctgcgaca
     1741 tttccttgaa agtccagatc tgcttgctcg accaattctt tgatagaata aaacatgctt
     1801 cctcctattt aaagaaattg acattgtgga gatgagggat ttttcgaatt tcttcgatag
     1861 cctcatcaca gttgcgactg tcaacttcga taatcataat ggctttttca ccagcttttt
     1921 cacgagtgac attcatctgg gcgatattga taccatagcg ggaaagcgcc tctgtaacaa
     1981 gggcaatcat acctggaata tcttgatgaa cgatgatgat agtcggtgta ttcatattga
     2041 gagagacggc aaaaccattg agttcggtta cctgaatatt tcctccaccg atagaaatac
     2101 cagtcacgct gatggtcttg tgggcatttt taacagtaat tttagtggtg ttagggtgag
     2161 gggcattgct gtctttctga atggtccaga caatcttgat accacgcttg tgggcaattt
     2221 ccagactatt tggaatttca ggatcatctg tatccattcc caaaatacct gcaacaaggg
     2281 ctaggtctgt tccgtgacca cgataggtct tggcaaatga gttaaaaagt tggaattcaa
     2341 cttctgtcgg agtatcatca aaaatggaag agacaatctt cccaatacga acagcaccag
     2401 cggtatggct actagatggg ccaatcataa ctggtccgat gatatcaaag acagattgaa
     2461 aacgaagtga tttcatcagt ttccccttat aaaaattctt atctctatta tatcaaagaa
     2521 tgaggggctt ggctttaatt gtggatgaaa acctttctaa tacctcaaat agcataaaaa
     2581 tagtatcttt tatg
//
LOCUS       DAVMAF010000137         2530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMAF010000137 DAVMAF010000000
VERSION     DAVMAF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.138"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            114..302
                     /locus_tag="VYO97_002050"
     CDS             114..302
                     /locus_tag="VYO97_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1754786.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            459..824
                     /locus_tag="VYO97_002051"
     CDS             459..824
                     /locus_tag="VYO97_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1754787.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            827..2494
                     /locus_tag="VYO97_002052"
     CDS             827..2494
                     /locus_tag="VYO97_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1754788.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT          813 a          462 c          583 g          672 t
ORIGIN      
        1 cacaaataaa aaatcttcca attcccttgc aaaaaacaag aaagtgtgat aacatagtac
       61 ggtatgtggt gctagcacat ccgctatatt agatctaata ggaggaaaac acaatggcta
      121 aagtatgtta ctttacaggt cgtaagactg tatcaggaaa caaccgttca cacgcgatga
      181 accaaacaaa acgtgccgta aaaccaaacc ttcaaaaagt tactgttctt atcgatggta
      241 aacctaaaaa agtttgggct tcagctcgtg ctttgaaatc aggtaaagtt gaacgcgttt
      301 aataataaaa gcaaaggaga ccttagggtc ttttttcttt tattgtgggt ataacatcgt
      361 ttgaaaaata gagtaaatat ccgctgatac atcattctgc ttttacactt gggctgaaat
      421 atgataaaat agagtatcaa ctagttgagg taaaaaggat gactgtaaaa attaatacaa
      481 aagatggtca aatcgaacta acagatgaag tgattgcaac cgtcgtaggt ggagcagcaa
      541 ctgagatttt tggtgtggtc ggtatggcta gtaaaaatgc cctcaaagat aatttccaag
      601 ccctgctagg taaggaaaat tattctaaag gtgtcgtcgt aaaggcagcc gaagatggca
      661 gtattgcagt tgatgtatat accgtgttaa gctacggaac aaagattagt gaagtgtcaa
      721 aaaacattca agagcgtgtt gcctttagct tggaaaacca gcttggaatt actgctcaga
      781 ctgtaaatgt ctacattcaa aatatcaaag ttgtaggaga ataaccgtgt caaagattac
      841 tactagctta tttcaagaaa tggtgcaggc tgcatcaact cgcttgaata agcaagctga
      901 atatgtcaat tcattaaacg tctttccagt tccagatgga gatactggga caaatatggg
      961 aatgaccatt gaaaatggtg ctaaagaagt tgcagacaag ccagcttcta cagttggaga
     1021 agtagcgagc attcttgcca aagggctttt gatgggtgcg cgtgggaact caggagtgat
     1081 tacatctcag cttttccgtg gattttccca agctatcaag gataaagacg agttaacagg
     1141 tcaagacttg gctctggcct tccaatcagg tgtggaagtt gcctataagg cagtgatgaa
     1201 acctgttgaa ggaacgattt tgacagtttc tcgtggagct gctatcggtg ctaagaaaaa
     1261 agctgagcaa acagatgata ctgttgaagt catgcgcgca gccttggaag gtgctaaaac
     1321 agctctagct aaaacaccag acatgcttcc agtattgaaa gaagttggcg ttgtggactc
     1381 aggtggtcaa ggattggtct tcatctacga aggtttcctt tcagccctta ctggcgaata
     1441 tattgcatct gaggactttg tagcgactcc tgccaacatg agtgagatga tcaatgtaga
     1501 gcatcataag tctgtagctg gtcacgtagc gactgaggac atcacgtttg gttactgtac
     1561 tgaaatcatg gtagctctta agcaaggtcc aacctatgct aaagattttg actacgatga
     1621 attccgtaac tacttggatg agcttgggga ttctctcctc gttgttaacg atgatgaaat
     1681 tgtcaaagtc catgttcata cagaagatcc aggacttgtt atgcaagaag gtctcaaata
     1741 tggtagcttg gtcaaggtaa aagttgacaa tatgcgtaac caacacgaag cacaggttga
     1801 gaaagaagct actcaagtta tcaagtcggc tgaagaaaaa gagtatgctt tgattgctgt
     1861 ggtggctggt aaaggtctag cagatatctt ctgttctcaa ggcgtggatt atgttatcga
     1921 aggcggtcaa accatgaacc cttcaacaga agactttatc aaggctgttg aacaggtcaa
     1981 tgcccgtaac atcatcttct tgccaaacaa caagaacatc ttcatggcag ctcaatctgc
     2041 ggcagaagtt ttggagcaac cagcagtagt ggtagaggct cgcactcttc ctcaaggtat
     2101 gacaagtctt cttgcctttg atccaagcaa gtccattgaa gaaaaccaag agcgtatgac
     2161 agctgctctt agcgatgttg ttagcggaag cgtcacaaca gccgtgcgtg atacaacgat
     2221 cgatggctta gaaatccatg aaaacgataa tctaggtatg gtggatggaa aaattcttgt
     2281 gtcaaaccct gatatgcacc aaacattgac tgaaaccttg aaacatatgt tggatgaaga
     2341 cagtgaaatc gtaaccttct atgtcggtga agacggaagc gaagaacttg ctaatgaaat
     2401 cgctcaagaa atcgtagaag aattcgaaga cgttgaagtc gagattcacc aaggtcaaca
     2461 acctgtttac ccatacctat ttagtgtgga ataagattaa tgaaggactg agaaatcagt
     2521 tcttttttct
//
LOCUS       DAVMAF010000138         2425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMAF010000138 DAVMAF010000000
VERSION     DAVMAF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.139"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            30..548
                     /locus_tag="VYO97_002053"
     CDS             30..548
                     /locus_tag="VYO97_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754789.1"
                     /translation="MNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKKDAHGIALK
                     LLSDPNREQFQHDNRQVELKILTRHIHRLKKKQSDWKVQYTRCLDIIFPELDKIVGKH
                     SEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASNSRAFCRALKSSPNHQIEIRNSLD
                     KTIDFSYSLSFS"
     gene            complement(514..1989)
                     /locus_tag="VYO97_002054"
     CDS             complement(514..1989)
                     /locus_tag="VYO97_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1754790.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          727 a          544 c          440 g          714 t
ORIGIN      
        1 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
       61 tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca cttcgcaaga
      121 ctaaaacgaa taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
      181 gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactgact agacatattc
      241 atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgt cttgatatca
      301 tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac caactcttga
      361 cacgctaccc taatcctcag aaaaggattg aggcaggatt tgataagctg atagaaatta
      421 agcgattgac cgcttctaat tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc
      481 atcaaattga aataagaaat agtttagata aaactattga cttttcttat agtttgtctt
      541 tctcttaata acgttgatat gattgacctc tggatcttcc gtcccttgga gggagcaacc
      601 acgttccttg gcgaattgga tatagtcgac aatttctcgt gtaatgcgtt caccaggagc
      661 caagatagga atccctggag gatagcacat gacaaattct ccacaaacct gtccaacaga
      721 atcatccaaa gttaaacttt ttcttgctga atagaaggct tcttgcggag acagcactaa
      781 ctcgggctga atatattctc ctgctatcaa atcttttcca tctcttgaat agagtctctt
      841 aatatcagcc agagcaccaa ccaagcgctc gatgtcttgg atgcggtcgc cgatagaaat
      901 ataggccaag atattgccga tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa
      961 gaggtcataa acctcgatac ctgttaagcc aataccctga gtgtaaactg acagcttagt
     1021 tacgtcaaaa tcgcaaaccg aaacaccgtc tattaattct tttgagtagg catagtagcc
     1081 accgatagca ttgatttcac ggcgggcata ctcagatagc tcaatgactt tctcaaacga
     1141 ctctttacca cgaagggcca agttgcgacg tgaaatatcc aaactagcca tcaacaagta
     1201 agaggcagat gtagactggg tcaggtttat aatctgacga acgtactcgg gattcatctg
     1261 ctccccgatt aaaagaagcg aactttgtgt caaactccca ccagacttat gcatggaaac
     1321 tgctgccata tcagctcctg catccatagc agaaattgga agtttaccag tgaaatgcaa
     1381 atgcgctcca tgggcttcat ctactaaaac catcatgcca gcttcatgag ccatttctgt
     1441 caaccccttt aggtctgaac agatgccgta gtaagtagga ttgttgatta ggatagcctt
     1501 agcatctgga tggtccttta tggcctgtgc tactcggtca ttttcaagac ctaaagcgat
     1561 accaatctta ggatctacac tcatctcgat atagatggga atggcaccac atagaaccaa
     1621 cgcattgata gcagatttat ggacatttcg tggcagaata atcttatctc ctgccttgca
     1681 ggttgccaga atcatagtct gcaccgatga agttgttcca ccaatcatta gaaaggcatg
     1741 gctagctcca aaagcatctg cagccagctc ctctgcatca cgaataatcg aaataggatg
     1801 gcctaaatta tccaagggtt tcatcgaatt gacatcaatg cctacacatt tttctcctaa
     1861 gagttcgaca agttctggat ttccccgtcc acgcttgtga cctggaacat caaagggaac
     1921 aatccttttc ttgcgtaact tcaccaaggc ttcataaatt ggggcttggt tttgatctaa
     1981 ctctttcaaa acatactcct ttcaatattt ttaggacctt atgaaaacta aggaactaaa
     2041 ggttgactca tgaaaaaaga gcaggttttc acctgctctg caatcttctg acgtgtttat
     2101 tttggtttct gttgagtatg tctgttcccg atgtttccta actctctagt agcaaatatt
     2161 acgtacttta ttgatcgttt cacaagatga cgtgtgcttt caccacacta aaaatttatg
     2221 aattaggaca agtgtcctaa catcaactta taaaagtagg cttttaaccc tatcaataat
     2281 gtggcttttc aaccacgctt cggcattcaa ccctgcctgt ccgtaggcat tttttactcg
     2341 ggtttatatt tgctagaaaa catcatctca ttttctaagc tttattacta tatcatgttt
     2401 ttaagaactt gtaaagtatt ttttg
//
LOCUS       DAVMAF010000139         2393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMAF010000139 DAVMAF010000000
VERSION     DAVMAF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.140"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VYO97_002055"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VYO97_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1754791.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2393)
                     /locus_tag="VYO97_002056"
                     /pseudo
     CDS             complement(1449..2393)
                     /locus_tag="VYO97_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          745 a          465 c          500 g          683 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaatccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggctct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaagt ttcgtccttt cttttttgat gttcagggcg
     1501 atgaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata
     1561 agtttgatga gattattggt cgcttccaat ttggcgtttg aatagggtag ttgaagggcg
     1621 ttgacgattt tctttttgtc ctttagaaag gttttaaaga cagtctgaaa aagaggatga
     1681 acctgcttaa gattgtcctc aatgagtccg aaaaatttct ccggttcctt attctgaaag
     1741 tgaaacagca agagttgata gagctgatag tggtgtttca agtcttccga ataactcaaa
     1801 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcgc
     1861 ttatcactca gtttacgact atcctgttgt atgagcttcc agtagcgctt gatagccttg
     1921 tattcatgag actttcgatg aaactgattc atgatttgga cacgcacacg actcatagta
     1981 cggctaagat gttgtacaat gtgaaagcga tccaacacga ttttagcatt cgggagtgaa
     2041 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcta
     2101 agtcatagta aggactaaac atatccatag taataatttt gacgcgacat cggacggctc
     2161 tatcatattt aagaaagtga tttcgaatga tagcttgtgt tcttccctca agaacagtga
     2221 tgatattgag attgttaaaa tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat
     2281 actcatccca agacataatc tcaggaagac aagaaaaatc atgtttaaag tgaaagtcat
     2341 tgagcttacg aataacagtt gaagttgaga tggaaagctg atgggcaata tca
//
LOCUS       DAVMAF010000140         2380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMAF010000140 DAVMAF010000000
VERSION     DAVMAF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.141"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            32..997
                     /locus_tag="VYO97_002057"
     CDS             32..997
                     /locus_tag="VYO97_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV1754792.1"
                     /translation="MKIIKKISIFALFVTFIFALVACGKTGLGNSSNDNKSTTQKSAK
                     ELKLGVSISTTNNPYFVAMKDGLEKAAGEKEVTLKIADAQDDAARQADDIQNFISQNV
                     DALLINPVDSDAIVTSIKAANNANIPVILIDRGSNGGEVLTTVASDNVEAGKMAAEFI
                     TKQLGEKAKTFELSGVPGASATVDRGKGFEQISKTNLDVLSSQSANFDRAKALNTAQN
                     MIQGNKEVQAIFAQNDEMALGAAQAVKAAGLSNVLIVGIDGQPDAHDAIAKGDITATI
                     AQQPAKMGEIAIQSAIDHYQGKKLEKETVSPIYLVTKDNVDQHNW"
     gene            1072..1215
                     /locus_tag="VYO97_002058"
                     /pseudo
     CDS             1072..1215
                     /locus_tag="VYO97_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            complement(1212..1727)
                     /locus_tag="VYO97_002059"
     CDS             complement(1212..1727)
                     /locus_tag="VYO97_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1754793.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLTIVDNGGRVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1739..2266)
                     /locus_tag="VYO97_002060"
     CDS             complement(1739..2266)
                     /locus_tag="VYO97_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1754794.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHIVFGTLGALYLRKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT          805 a          392 c          477 g          706 t
ORIGIN      
        1 cctgtagata tatattacga ggagtaataa catgaaaatt attaaaaaaa ttagcatttt
       61 tgctttgttt gtaacattta tttttgcttt agtagcttgt ggaaaaactg gtttaggaaa
      121 ttcatcaaat gataataaat ctacgacaca aaagtcggca aaagaattaa aattaggagt
      181 ttctatttct actactaaca atccttattt tgtggctatg aaagatggtc ttgaaaaagc
      241 tgcaggggaa aaagaagtaa ctttgaagat agcagatgca caagatgatg ctgctagaca
      301 agcagatgat attcaaaatt ttattagtca aaatgtagat gctttgctaa ttaatccagt
      361 tgattctgat gcaattgtga catctattaa agctgctaat aatgcaaaca ttccagtaat
      421 cttaattgac cgcggtagta atggtggtga agtcttgaca actgttgctt ctgataatgt
      481 agaagcgggt aaaatggctg ctgaatttat taccaagcaa ttgggagaaa aagcaaaaac
      541 ttttgaatta tcaggggtcc caggcgcttc tgcaacagta gatagaggta aaggatttga
      601 acaaatttca aagacaaatt tagatgttct ttctagtcaa tctgctaatt ttgatcgtgc
      661 aaaagctttg aatacagcgc aaaatatgat tcaaggaaac aaagaagtac aagcgatttt
      721 cgcacagaat gatgagatgg cattgggagc tgcacaagca gtaaaagcag ctggtcttag
      781 caatgtatta attgttggta tcgatggtca accagatgca catgatgcta ttgcaaaagg
      841 tgatattact gccactattg ctcagcaacc agctaagatg ggtgaaatcg ccattcaatc
      901 agcaattgat cattatcaag gaaaaaaact agaaaaagaa acagtttctc caatttatct
      961 tgtgactaag gataatgttg atcaacataa ctggtgatat atttttaata agtagatact
     1021 tattcatctt cattcaatgt agtttttaaa tatcattgct actatttgaa ctcttgggtg
     1081 caaaaagacc agattccaaa cttggatctg gcctatgata tgttaccctt gatggatatg
     1141 atggaagctc ctgacaaatc tgaatttttc taccctcgcc gtacagaaga tgattgggaa
     1201 aagaaaattt tctaatcctt tactaaataa cctagctgat ccaaggcctc ctcgatatag
     1261 tggaggtctt gttgtgtttc ggcttcgact aggtggtaat ggatgccatc tgttaattca
     1321 gacagaggtc taaaatctga atgctcgact tgttctagaa aatgttgcac atctctacga
     1381 caagacagtt tcagcaaggt ttcaatttct ccatagacag gatggtcaat caaagtgttt
     1441 tgaacacgtc caccattatc tacgatagta aggagttcct gaccgatttc ttcaacttca
     1501 tgtttcactt tgaatagttt gtgaacataa ggattggtat caatttgctt atagatgtag
     1561 ccacgattag tggataggat aggagcgcca tcggctctta aaattgcaat gtcttgtaca
     1621 atgacctgac gtgtgacatg aaaatgttca gctaaacttt ggccattaag ggctttagga
     1681 gcttctttca agagttggag cagggcttgt ttgcgatctt ttgtcatagt tcttcctttt
     1741 aacggcgttt tcgaagcact ttatagacag ctagtgctaa tgtatagtct accatactat
     1801 ggataattgt gccaaatcca actagcacaa agagaacata aaacatattt tctacattgg
     1861 taccagaagt tgcgtaaaaa acgacacagg ctaatacttc agcaagtgca tgaacaacag
     1921 ccaaaacaaa gttgaaaacc caggaagatt ttggtttatc tagggtatcg gggaattttc
     1981 gtaggtaaag agctcctagg gtaccaaaaa cgatatgaga gaaagctcta aaaacaataa
     2041 ccatgggata gccagccatc aaaaatccaa aactagaagc taggatgaca aaaactgcca
     2101 tcaagggcga caagaacatg gctataaaaa tagcgatgtg gctccccaaa gtataggaag
     2161 caggtggaat gacaatcttg aaaggcataa caattggaat caaaatcgca atagccgtta
     2221 aaagggctgt cattgtcata aattgtgtct ttttccgtgt attcacaaga atctcctttt
     2281 taactgcata tacactagta tggtacaata aaccagacaa taaagcaaga atttacttgg
     2341 gtttatagat cattttttag ttaaaagtta tagtggattg
//
LOCUS       DAVMAF010000141         2289 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMAF010000141 DAVMAF010000000
VERSION     DAVMAF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.142"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(191..904)
                     /locus_tag="VYO97_002061"
     CDS             complement(191..904)
                     /locus_tag="VYO97_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1754795.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1394..1501)
                     /locus_tag="VYO97_002062"
                     /pseudo
     CDS             complement(1394..1501)
                     /locus_tag="VYO97_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1923..>2289)
                     /locus_tag="VYO97_002063"
     CDS             complement(1923..>2289)
                     /locus_tag="VYO97_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1754796.1"
                     /translation="RNKNGQADTNQTEKPQTEKPEEETPREEKPQSEKPESPKPTEEP
                     EEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQD
                     NNTIMAEAEKLLALLKESK"
BASE COUNT          702 a          439 c          364 g          784 t
ORIGIN      
        1 taaaaattaa atcgccagag aaatttttct tatcaaaaaa cttaaaaaga gattgataaa
       61 tactatttac taattgacaa gtgtcagatg taaattcata attaacagct tcattaaaat
      121 caacttcaga catttcaatg tactcttcaa tagtaatatt agcttttttg tagtcattag
      181 ctcgtctcct ttaaaatgtg atcttttcaa gaatattttt atctaaacaa tccagcaagt
      241 cttggtaaga atagacttcg taagtcggct gggcttgtgt gtgattttcg aggtgatgag
      301 gattatacca gatagtgtca atccccgcat tattgccacc ttgaatgtcg gcggttagag
      361 aatctccaat catcagcgtc ttttctttac taaatccagc aatttgctgg ccaatctttt
      421 cataaaaaag agcatccggc ttttgagttt gcaactgttc tgagataaag acttgattga
      481 aataaggtgc tagaccagat tgagccaaac gtcctgtctg aatggcagta atgccatttg
      541 tcgcagcata caagttataa tcacgctcaa tgaggctgtc caagagatca tgagcgcccg
      601 atagtgtttg tccctgctgg gcgaggtaaa attggtaacg ctgggcaaga aaactaccgt
      661 ctttttcctg tccaaaatga gcaaataaac gagaaaagcg cgtgttaacc agctcttgtt
      721 tactgatttt cttcagctcc aagtctttcc agagagcctt gttcatagga acgtaataat
      781 ctttataagc ctgaatatcc gcaactcctt cttcttttag aagttgggtc aaagccacat
      841 cctcagcagc atcaaaatca agaagagtgt ggtcgaggtc gaagagtaga aatttgtaga
      901 acaatttgag gttttccttt ctgaaaattc attaagaaca ttatatcata aagcacctca
      961 tacaattaac taatttaatc acttaaaaaa aattcgaaca ctttctatac aactgacagc
     1021 tcaaatcttt cagaatagaa caatactaac tatcgaacac cccgtcttca taaatacata
     1081 tgtaattcta ggcctagaat tcctataaac taaatgcttt catactcttc caagtaattg
     1141 attgccttaa attttaattt ttgaaggttt ctaaagctag aatagcccca tcacaatcag
     1201 ttttgattga ttcacaattt agaaacacta tagtttcact cctgttaaaa taaaaaggaa
     1261 ctgcataaag caatcccttt ctgattttga aatcatttac ttaacatttt atagttgaga
     1321 taatcaatag cttatctata aaaagagtta tagtaaaatt ccttatttat tgatttcaag
     1381 ctccgctaac tgtataagta atcggaacat ctttagctac tttactaatc agttcaacaa
     1441 caccttctgc aatgtgtttg gaatgtgaaa cgataacaag accaatacca atactttcca
     1501 tcaaaccacc ccagtttcta aaatagcagt aaagagtaat cctgatgaga atgatccagg
     1561 atcaatatgt ccaagaaacc acatgctcct aagacaagag ctaacagact ggccatcaat
     1621 aatagtattg ttcttttttt catcattact ccttaactag tgtttaactg attaattagc
     1681 cagtaaatag tttatcttta tttacactat ctgttaagat atagtaaaat gaaataagaa
     1741 caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtatact attctagttt
     1801 caatctacta tattttgcac attttcatga aaaaaatgag aactagaact cacattctgc
     1861 tctcattttt cgttttcccg ttctcctatc ctgtttttag gagttagaaa atgctgctac
     1921 ctttacttac tctcctttaa taaagccaat agtttttcag cttctgccat aatagtattg
     1981 ttgtcctggg tgccaaatag taaattattt tttaatcctg tgagagtctc tttggcattg
     2041 gacttgataa ttggatcctg gatttttcca agtaaatctt cagcctctct cagtttttct
     2101 tcaacctttt cagtctcgac ctgaggttct tctgattcct ctggtgattc ttcttctggt
     2161 tcctctgttg gttttggaga ctctggtttc tcgctttgcg gtttctcttc tcgaggagtt
     2221 tcttcctcag gtttttctgt ctgaggtttc tccgtttgat tggtatcagc ttgaccattt
     2281 ttgtttctt
//
LOCUS       DAVMAF010000142         2181 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMAF010000142 DAVMAF010000000
VERSION     DAVMAF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.143"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..863
                     /locus_tag="VYO97_002064"
     CDS             <1..863
                     /locus_tag="VYO97_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754797.1"
                     /translation="SLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDID
                     LKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILI
                     DGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTD
                     FIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKI
                     QESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIE
                     KTKMAEEFIY"
     gene            874..1503
                     /locus_tag="VYO97_002065"
     CDS             874..1503
                     /locus_tag="VYO97_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1754798.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGSYCVGYFTPCLILWCYLN"
     gene            1404..1961
                     /locus_tag="VYO97_002066"
     CDS             1404..1961
                     /locus_tag="VYO97_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1754799.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFIAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGISFKNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            1949..>2181
                     /locus_tag="VYO97_002067"
     CDS             1949..>2181
                     /locus_tag="VYO97_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754800.1"
                     /translation="MVEIKNLSLDYGEERILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQ"
BASE COUNT          795 a          275 c          434 g          677 t
ORIGIN      
        1 actctttaga tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag
       61 aaagattaaa agaaattcaa aatcaagatt tacaagaagg cgatgactat agcttaaaaa
      121 aatttgatat tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa
      181 atggtgtaag ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct
      241 gcggtaaaac aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa
      301 tcttaatcga tggcaaagat ataaaagaaa tatcaacaga atctcttttt gataaggtct
      361 ctatagtttt ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg
      421 gaaagcaaga tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag
      481 attttataga aaaaatggat aaaggtttcg atacagttat tggtgagaac ggagctgagc
      541 tatcaggagg agaaagacaa agattatcaa tagccagagc cttcttaaaa gatgcgccga
      601 tattgatctt agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag
      661 agtctttaaa taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat
      721 ccatagaaaa tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta
      781 agcatgaaga gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa
      841 tggcagaaga atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa
      901 gatttagtaa gcatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt
      961 atgatgggct tgattccaat attgttctta atatacccga cagtattagc catagttgca
     1021 ggaactgttg ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt
     1081 ggtatgatat caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta
     1141 tcacttatag taatgataat agcagaatta attagaaaga ttggtaatta taattcattt
     1201 aaatacaata tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa
     1261 atgcttttag caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat
     1321 gttgatgtgt tagaaaagtt aataacttat cctcatatgg ctttagtagc cttaggtgct
     1381 ttcttaggag gaattcttgg agcatatata ggcaaggctc tattgaaaaa acacttttca
     1441 aatggatcat attgtgtggg atactttact ccttgcctaa ttttatggtg ttatctgaat
     1501 taaaccctat agttaagatg tttttgagta tacctattgt tattagaatg tttattttac
     1561 catttatagc agcaagcttt atgataaaga cctcggatgt aggcgcaata atttcatcga
     1621 tggataagct taagatttca aagaatgtat ccatacctat tgcggttatg tttagattct
     1681 tcccatcttt taaggaggag aagaaaaaca tcaaaatggc tatgagagta agagggataa
     1741 gttttaaaaa cccagtcaaa tatcttgaat atgtttctat gccactactc attatatcat
     1801 ctaatatatc agatgacatt gcaaaagcgg cagaaacaaa ggcaatagaa aatccaattg
     1861 ccaagaccag atacattcgc gtaaagatac agctaattga ttttgtttat gttttagcgg
     1921 ttgctggact tattgtggga ggcttaatat ggttgaaata aaaaatttaa gtcttgatta
     1981 tggtgaagag cgtatattag atgatatatc actatccata gccgagggag agtgcgtgct
     2041 atttacagga aaaagtggaa atggtaagtc atctttaata aattcaataa atggactagc
     2101 tgtaaggtat gataacgcaa agacaaaggg cgaaataatt attgatggta agaatataaa
     2161 aaatttggaa ctttatcaaa t
//
LOCUS       DAVMAF010000143         2127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMAF010000143 DAVMAF010000000
VERSION     DAVMAF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.144"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..201
                     /locus_tag="VYO97_002068"
     CDS             <1..201
                     /locus_tag="VYO97_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1754801.1"
                     /translation="NANGSMATGWLKDGDTWYYLEASGAMKESQWFKVSDKWYYVNGS
                     GALAVNTTVGGYRVNANGKWVN"
     gene            541..>2127
                     /gene="pspC"
                     /locus_tag="VYO97_002069"
     CDS             541..>2127
                     /gene="pspC"
                     /locus_tag="VYO97_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV1754802.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEREGST
                     QAATSSKMANESQIERRKAAEQAIVSLSGYMTSLLNDGLDTSDSRFEELLKRAKGIVE
                     EYRKKIDGASDREKIEKLQQEGQTKLDELVDKFKKGLSSSEQNGHATPKDPSRNDGVQ
                     GDDSSVGQGEQPGASNQKNPESVPPTASGETDSPTKPKTSNDSVNSLERELKEVKETA
                     KTTLNTYMLTRLKQENPGVFWFADLLRESKESVEKYKRMFDEASSKENVENLVKEAKQ
                     EIEALVVKHKGREIDLERTKAKTVIAKYLTGLLDDIKKNLKKEQHINTVELIKKLGDI
                     KRTYLYKLDESTQKAQLQELVTESQSKLDEAFSKFKNGLSSSSSSGSSTKPETPQPEK
                     PEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKILDDIQKHHLQK
                     EKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFKKGLTQD
                     TPKEPGNKKPSAPKPGMQPSP"
BASE COUNT          804 a          359 c          439 g          525 t
ORIGIN      
        1 aatgctaacg gttcgatggc aacaggttgg ctgaaagatg gagatacatg gtactatctt
       61 gaagcatcag gggctatgaa agaaagccaa tggttcaaag tatcagacaa atggtactat
      121 gtcaatggct caggtgccct tgcagtcaac acaactgtag gtggctatag agtcaatgcc
      181 aatggtaaat gggtaaacta aacctaatgt aactcgttaa tgctggcttc ctgtaagaac
      241 tctttaaagt attccctaca aataccatat cctttcagta gatactatac ccttgtagga
      301 agtttagatt aaaaaataac tctgtaatct ctagctggat ttatggcgct agagactacg
      361 gagtttaatt tctgttgaat tctagtttca ttagaatagc agaggaagaa aaattattgc
      421 aggaagttat tattccaaat ggaagaataa aaggatagcg aaaataatgt agcaaatttc
      481 atatacaatt catattagca ggatatactg tagttaatta aaattatgga gaatatagac
      541 atgtttaaat caaatcatga gcgaagaatg cgttattcca ttcgtaaatt tagtgtagga
      601 gtagctagcg tagctgttgc cagtcttttt atgggaagtg ttgtacatgc gacagagaga
      661 gagggaagta cccaagcagc cacttcttct aagatggcaa atgaaagtca gatagaacgt
      721 aggaaagctg ctgaacaagc tatcgttagt ttgtctggtt atatgactag tcttctaaat
      781 gatggattag atacttctga ttctagattt gaagagcttc tcaagcgagc taagggaatt
      841 gtagaagagt accggaagaa aattgatgga gcttctgata gagaaaaaat agagaaactg
      901 cagcaagagg gacaaacgaa gctagatgaa ttggtagaca aatttaaaaa agggttgtcc
      961 agctctgagc aaaatggtca tgctactcct aaggatccct ctcgaaatga tggtgtacag
     1021 ggagatgatt cttcagtagg gcaaggagaa cagccaggcg catctaatca aaaaaatcca
     1081 gaatctgtac caccaacagc atctggtgag acggattccc ctacaaaacc aaagacttcg
     1141 aatgattcag tgaattctct ggagagagag cttaaagaag ttaaagaaac tgctaaaaca
     1201 actttgaaca cttatatgct tactcgttta aaacaggaga atccaggagt tttttggttt
     1261 gcagatcttt taagagaatc caaagaatct gtagagaagt ataaaaggat gtttgatgag
     1321 gcttcgagta aagaaaacgt ggaaaacttg gtaaaagaag ctaaacagga gatagaagct
     1381 ctagtcgtta aacataaggg gcgagaaatt gacttagaac gaacgaaagc aaaaaccgta
     1441 attgctaaat atttaacagg attattagat gacatcaaga aaaatttaaa aaaagagcaa
     1501 catatcaata ctgtagagct aattaaaaag ctgggagaca ttaagcgaac gtatttgtat
     1561 aaattagatg aatcaaccca aaaagcccaa ctacaggaac tggtcacaga aagtcaatca
     1621 aaactagatg aagctttttc taaatttaaa aatggcttat cttcttcgtc aagttcagga
     1681 tcctccacta aaccagaaac tccgcagccg gaaaaaccag agcatcaaaa accaacaact
     1741 ccagctccgg ataccaaacc aagccctcaa ccagaaggca agaaaccaag cgtaccagac
     1801 attaatcagg aaaaagaaaa agctaagctt gctgtagcaa cctacatgag caagatttta
     1861 gatgatatac aaaaacatca tctgcagaaa gaaaaacatc gtcagattgt tgctcttatt
     1921 aaggagcttg atgagcttaa aaagcaagct ctttctgaaa ttgataatgt aaataccaaa
     1981 gtagaaattg aaaatacagt ccacaagata tttgcagaca tggatgcagt tgtgactaaa
     2041 ttcaaaaaag gcttaactca ggacacacca aaagaaccag gtaacaaaaa accatctgct
     2101 ccaaaaccag gtatgcaacc aagtcct
//
LOCUS       DAVMAF010000144         2103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVMAF010000144 DAVMAF010000000
VERSION     DAVMAF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.145"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            51..371
                     /locus_tag="VYO97_002070"
     CDS             51..371
                     /locus_tag="VYO97_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1754803.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            417..725
                     /locus_tag="VYO97_002071"
     CDS             417..725
                     /locus_tag="VYO97_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1754804.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            742..2040
                     /locus_tag="VYO97_002072"
     CDS             742..2040
                     /locus_tag="VYO97_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1754805.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
BASE COUNT          641 a          290 c          442 g          730 t
ORIGIN      
        1 aaaatttcaa atgaaaaaag ctttttacat agtgaaatga ggaggaattt atggaaatta
       61 ttgttccaga tcaaattatc atgggtttaa ttttatatgc tggcgatgcg aaacaacata
      121 tttataaagc attagattac ataaagaacg gtacatgtga acgatgtgaa gaagaaatcc
      181 agttagctga tgcagcctta ttagaagctc ataatctaca aacaaaattc ttggcacagg
      241 aagcgtctgg tacaaagaca gaaattacag ctctctttgt tcattcacaa gatcatctca
      301 tgactagtat gacggagatt aatttaatca aagaaattat tagtttgaga aaagaacttc
      361 ataaaaaata atactagagt attatcattg ttattaacat agaggaggaa aacataatgg
      421 tgaagattgg tttgttttgt gcagcaggtt tttctactgg tatgcttgta aataatatga
      481 aaattgcagc gcaatctagt ggagttgagg cagaaataga ggcgttttct cagtctaaat
      541 tagcggatta tgcgccaaat atagatgttg cactattggg tccacaagtt gcttatacat
      601 tagataaatc aaaagaaatt tgtgataagt gtgatgttcc gatagctgtt attccgatga
      661 tggactatgg tatgttagat gggaaaaaag tattagattt ggccctatct ttgattagtg
      721 ggtaagaaaa ggagatttat tatgtcaaag atggatgttc agaaaatcat tgcaccgatg
      781 atgaagtttg tgaatatgcg tggcattata gctctaaaag atgggatgtt agcaattttg
      841 ccattgacag tagttggtag tttgttcttg attatgggac aattgccgtt cgaaggatta
      901 aataagagca ttgctagtgt ttttggagct aattggacag agccgtttat gcaagtatat
      961 tcaggaactt ttgctattat gggtctaatt tcttgttttt caattgccta ttcttatgct
     1021 aagaatagcg gagtagaggc tttaccagct ggagttctat ctgtatctgc attctttatt
     1081 ttgctaagat catcttatat ccctaaacaa ggtgaggcga ttggggacgc tattagtaaa
     1141 gtttggtttg gaggccaagg aattatcggt gctatcatta taggtttggt agtaggaagt
     1201 atttatacct tctttataaa gagaaaaatt gttattaaga tgccagaaca agttccacaa
     1261 gctattgcca aacagtttga agcaatgatt ccagcatttg taattttctt atcttctatg
     1321 attgtatata ttttagcgaa gtcattgact aatggcggaa cattcataga aatgatttat
     1381 tctgctattc aagttccgtt gcaaggttta actggatctt tgtatggtgc tattggaatt
     1441 gcattcttta tatcattttt gtggtggttt ggtgttcatg ggcaatcggt agtaaatgga
     1501 gtagtgacag ctctgctttt atctaatctt gatgctaata aagctatgtt agcctctggt
     1561 aatctatcat tagaaaatgg tgcacatatt gttactcaac aatttttaga ttcattttta
     1621 attctatcag gttcagggat tacgtttggt cttgtagttg ccatgctttt tgcagcaaaa
     1681 tcaaaacaat accaagcctt aggaaaagtt gcagcttttc cagcaatatt taacgtaaat
     1741 gagccagttg tatttggatt tccgattgtc atgaatccag ttatgtttgt acctttcatt
     1801 cttgttcctg tacttgcagc tgtgatagta tatggagcta ttgcaacagg tttcatgcag
     1861 ccattctcag gggtaacatt gccttggagt acaccagcta ttctatcagg atttttggtg
     1921 ggtggatggc aaggtgttgt gactcagtta ttagtattgg tgatatctac agtggtttac
     1981 tttccattct ttaagataca agatcgttta gcttaccaaa atgaaatcaa acaatcttag
     2041 aggtatttgt gtgttactgt taaactcaca catttgtgct aaaaattaga gagttaaaat
     2101 ttt
//
LOCUS       DAVMAF010000145         1914 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVMAF010000145 DAVMAF010000000
VERSION     DAVMAF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.146"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            25..870
                     /locus_tag="VYO97_002073"
     CDS             25..870
                     /locus_tag="VYO97_002073"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1754806.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1017..1719)
                     /locus_tag="VYO97_002074"
                     /pseudo
     CDS             complement(1017..1719)
                     /locus_tag="VYO97_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          493 a          382 c          382 g          657 t
ORIGIN      
        1 taaatataat ataaggagaa atatatgtat cttattgaaa ttttaaaatc tatcttcttc
       61 gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca cttgatttta
      121 gcagaggagt ttatccaata ccaaaatcaa aatgaagcct ttatgtccat gtttaatgtc
      181 gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa gctcaatcct
      241 tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg gttgaaggtc
      301 ttgattgcca ctttgccttt acttggtgtc tttaaatttg atgattggtt tgatacccac
      361 ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc cttcatctat
      421 ttggaaaagc gcaataaagc gcgtgctatc gagccaagtg taacagagtt ggacaagctt
      481 ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt accagggact
      541 agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg ttcagttgtg
      601 acagaattta ccttctatct tgggattccc gttatgtttg gagctagtgc cttaaagatt
      661 ttcaaatttg tgaaagccgg agaactcttg agctttgggc aattgttttt gctcttggtc
      721 gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac cagctatgtg
      781 aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag tgttttgcta
      841 ctttacagtt ttgtccgttt atttgtataa gaaaaacctt gaaggggcaa ctcttcaagg
      901 ttttatactc ttagaaaatc tcttcaaacc gcgtcagctt tatctgcaac ctcaaaacag
      961 tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat tgagctttaa
     1021 aatccagtca gggtaatccc caataggcgg acacctcttt ctttctcgct taattcttca
     1081 tagagttgca gggctatttg gcttatctga ctagcatctt gtgttttttg agcaagactt
     1141 tttcgtttgg taagagttga aaagtcctcg tagcggattt tcaaaatgac aatttttcca
     1201 gctttttctt gttgatgtag attgagagcg actttttctg atagaagagt cagctctttt
     1261 ttgatatctt cctcagcacg gagaatcttc ccgtaggttt tctccttgcc gattgattta
     1321 cggatgcgat tggatttgac tggagagttg tgaatgccac gagcctttcg atacagatca
     1381 tagcctagtc taccaatctg tatagcgctt aaaaatagct cgaatctgga gtcccacaga
     1441 cttgtatttc tcataattcc ctgagataaa gacagcctgg ggacaacgtt cataagcttc
     1501 cttggaactc atggcagaat ggacaccaaa agctcttgcc tcataactac aggtagaaac
     1561 gactccccgt ccacctgttt gccgagggtc gcttccaata atgacaggtt ttcctctgag
     1621 tttaggatta tccctgattt ccactgcagc aaaaaaggca tccatgtcaa tatggatgat
     1681 ttttcttgac aaatcattta acaaaggaaa aatcaacatg cctagcacct ttttatactc
     1741 ttcgaaaatc tcttcaaacc acgtcagctc tatctgcaac ctcaaaacag tgttttgagc
     1801 aatctgcggc tagcttccta gtttgctttt cgatttccat tgagtgttac tgcttatttt
     1861 cttttattat accctttttt ctgaaaaaaa gaaaaaagga ctttattttt tcaa
//
LOCUS       DAVMAF010000146         1830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVMAF010000146 DAVMAF010000000
VERSION     DAVMAF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.147"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(134..1765)
                     /locus_tag="VYO97_002075"
     CDS             complement(134..1765)
                     /locus_tag="VYO97_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1754807.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          557 a          364 c          385 g          524 t
ORIGIN      
        1 tagaaataga ggtgtactat tctagtttca atatactata tatctgcaga atactctttc
       61 agcttcattc agcctagtct tgcttaggaa aaagtaaaaa gccatccaat tatgatggat
      121 agcttcttgg ttcttagatt tgttctgcaa aaaccttttc agcaaggttc atagcgtggt
      181 ctgatacacg agtgtagtgt gagatgatgt cgataaagtt gaccccagct tgtgttgaac
      241 attcgccttt gttgaggcgt ttgatgtggg tttttctgag aacacgttct atcttattga
      301 ttgcttcatg acgttctaca agactgcgtg ctttttcaat atcattgttt tccacactat
      361 ccagagcatc tttgataaag tcactagttt ggcggtaaac ttcctctaat tctttcaagg
      421 cggcatcaga aaattcaaca tttttccgtt gaagatagtc agtcagattg agtagagcct
      481 ccgtgtggtc tccaatccgt tccaaatcac gggaggaatc aaggatattg gtgagcactt
      541 cactttcttt ttggctgaga gcttcgcttg aaagggcaat gagataacgt gttaattgct
      601 catcgatggt gttaattgct tcttcggttt tatgcccttt ttcagcaact ttttcatcca
      661 agtcaatgat gtacttatag gaaaggtcaa aggctttagc agcgtagttt cctaagtgca
      721 agagctcttt tttagcattt cctagagcga tagatggggc ttgtttgatg aaatgttcat
      781 caagatataa gggttcgtat ttgacaacct cgtcctctcc aggaataatc ttggttacaa
      841 agtaagccag agctccgata aatggaaatt ggacaatggt gttggtaata ttaaaggttc
      901 cgtgagcaaa ggcgatggtc atttccggtg ctagatttag cgtagcttca aaccaatgaa
      961 tcaggacagt aaaaggaact agaaaaataa cgcagacaac tgttccgata acgttgaagg
     1021 caacatgagc tcctgctacc cgtttagctg caatattagc ccctaaagag gcaatgatgg
     1081 ctgtaatggt tgtcccgata ttgtcaccaa atagaactgg caaagctccc tgtaggtcaa
     1141 ttagattgcc ggcgtagagg ttttgtaaaa tcccaatggt agccgaagaa gcttgaatta
     1201 gcaaggtcaa gccagtaccg acaaagacac ccaaaacagg attcttactt agctcaatca
     1261 tatagtcctt aaagacctgt aaatccttga gtggagccat tgcgccgctc ataagattga
     1321 gggcaaaaaa gataccaccg acaccaaaga ggatgcgtcc gatattattg actgtccgat
     1381 ttttcgtaaa aaagagacag acggcaccga taaagagcat aggtagggca tagttaccta
     1441 atttaaaacc gatgagaaag gatgtgacag ttgtcccaat attagcaccc atgacaatcc
     1501 cgatagcctg acgtaaggtt aagagaccgg cactgaccag gccgactgtg ataactgtta
     1561 caccagaact agactgaatt agagcagtca tcccaatacc aaccagaact ccaaagaaag
     1621 gattactagt atatttgtca atgtaaaaac gaaggcgatc tccagcagct tgttgcaaac
     1681 cgtctcccat ggtcttgata ctatataaga atagccccag accacctaaa aagtgaaata
     1741 aaatttcctg ccaattaatg gacatttctt tttcctccga aaaataatcg cggaatatct
     1801 cctattctat tttaaaggat aaaagtaaat
//
LOCUS       DAVMAF010000147         1750 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVMAF010000147 DAVMAF010000000
VERSION     DAVMAF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.148"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            500..670
                     /locus_tag="VYO97_002076"
     CDS             500..670
                     /locus_tag="VYO97_002076"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754808.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEKAG"
     gene            1282..1566
                     /locus_tag="VYO97_002077"
     CDS             1282..1566
                     /locus_tag="VYO97_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754809.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1559..1708
                     /locus_tag="VYO97_002078"
     CDS             1559..1708
                     /locus_tag="VYO97_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754810.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
BASE COUNT          655 a          303 c          263 g          529 t
ORIGIN      
        1 gctgcaggct gctcaaaaca ctgttttgag cttgtagata gaactgacga agtcagtaac
       61 atctatacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaaaag tattagttaa
      121 atcttgttta atatctctac accatactat tttacatata ttatttagcg ccacatagta
      181 ctgaactctc tccaaaaacg gttatgcctc tttgaatagg gcgttatcac aagaaaagca
      241 tctccacgtt tcaacttcat atggctcaaa aacaatcaat tgatgctaaa acctgtacct
      301 agatgtttcg gttcataaaa ccatgaaact gtaaaagtgg atgaaattga tagcgatagt
      361 caaatcaaga ggcatcataa ctctaaaaag tcacaatata taagttcatc ctcggaaaaa
      421 tatcattcta attgttgaaa tgcctacatg aaaagaaacg tcaaatgctc atgaaacaac
      481 gaatacaggt atcaaaacta tgacaaaaca agtccctaaa tttactaaag acactgctca
      541 actttacacc tgtaaatggt tgttgtataa taaagttaca aagatgtacg accacactgt
      601 tgtaaatcat agtgttcgcg aatatattac tgatagcatt tctacaaata caattgaaaa
      661 ggctggatag gctttaaacg aaatatgtca gtgttttggc attcctagcc ttcatatcat
      721 ttaaagaatt gtatagacaa aatttttccc aatacagaca ctcgtaacaa ctgcttcatt
      781 tttctaccaa catatttagg aacaggataa gatacaagag gattaatcca tagatcagtt
      841 ctataccaat ctaagacaaa taagctaaaa aaacgattga taataagcaa atagattcca
      901 aattttctct atctgctcat tttaataaac aatactagtg taactatcct tccagtcaga
      961 agcttgtcaa atcacaccga aaattcttct aaaatttatc tcgttaggca atcaagcaaa
     1021 aactcgacga tagtacaaac attatcatac agaattgact tcctaaatta tatactttag
     1081 taatgttttc ggataagaaa aaagattcat tttacatttc taaacattct tttctaagat
     1141 gaaaaacaga atttttcgat tgtgatttaa agcaacaaga agattttcag tatcatccta
     1201 tagatacgag ctaattaaga aaaactacat tttcgaatat aaactacaat aatataaact
     1261 aaattttata ggaggaagac aatggattgg tacgattata tgatacaggc atccaaacaa
     1321 tcacaattca acgcaagcca ttggtttcgc tatttgcgaa aagttatttt tgaagactat
     1381 tcttatttaa caaaccaaga tgtagaaaag ttgctagact ccaaagaact aacccgtttt
     1441 caaaaaatta gcttgaagta tgcctttcaa gagcatactc caactcataa atatgtgatt
     1501 tcattaaata aacctgctaa gttaaccaat gttcaaaaat tgatggagaa atacaaacat
     1561 ggataaaatg aaaccggtct tccaagccct aaataaggaa ttaattcagg aaaatctgac
     1621 tttaacaatt atctgtgtcg atggttatgt cttagaatat catggtttac gtgccacaca
     1681 agatgttgat gcttttatgg ctctataata tttgtagtgg gtaaatcccc tatggatatt
     1741 atggagccta
//
LOCUS       DAVMAF010000148         1730 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVMAF010000148 DAVMAF010000000
VERSION     DAVMAF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.149"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..643
                     /locus_tag="VYO97_002079"
     CDS             <1..643
                     /locus_tag="VYO97_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1754811.1"
                     /translation="DLDKLESKGLLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEA
                     VKLVSDNETIMIESGSTCALLAEEICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGG
                     EFQKDSQVTVGPLLKEMIQTFHVHQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASD
                     KVIVLTDSSKFDKRGTVRRFALSQVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            656..1636
                     /locus_tag="VYO97_002080"
     CDS             656..1636
                     /locus_tag="VYO97_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1754812.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          628 a          188 c          350 g          564 t
ORIGIN      
        1 agatttagat aaattagagt caaaaggttt attacacaga gagcatggat atgctgtatt
       61 aaatagtgga gatgacttaa atgtacgttt gtcaattaat tatgaaatta agagaaaaat
      121 tgttcaggaa gcagtaaaat tggtgtcaga taatgaaaca ataatgatag aatctggatc
      181 gacctgtgct ttacttgctg aggaaatttg caagcaaaaa agaaatgtta cgattgtaac
      241 aaattcattt tttatagcaa attttgtgag agcttatgat tcatgtcgta ttattgttct
      301 tggtggagag tttcagaagg attcacaggt tactgtggga cctttattaa aagaaatgat
      361 acagactttt catgtgcatc aagcttttgt tgggacagat ggttacgata aagagatggg
      421 ctttacagga aaagatttaa tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga
      481 taaagtcatt gtactaactg actcaagtaa atttgataaa aggggtacag taagaagatt
      541 tgctttaagt caagtctatg aagtaataac agacgaaaaa ctttctaaac aaaatatagc
      601 tacattagaa aatgctggga taatggttaa ggtagtttcg taagaggtta agtgtatgaa
      661 tcaagatagg aataaactgc tctctaaaat tgcttatctg tattatattg aaaacttaaa
      721 tcagtcacaa atagcagcaa aattaggaat ttatagaacc tctattagta gaatgttaac
      781 agaagcaagg aatgcaggaa ttgttaaaat tgaaatagag aattttgata ccaatatgtt
      841 taagttggaa aattatgtaa aagaaaaata tggtctggaa agtttagaaa ttattccaaa
      901 tgaatttgat gataatccaa caattttatc tgaaagaatt tctcaagttg cagcaggtgt
      961 ccttaggaat ctaattgatg ataatatgaa aattggcttt tcttggggga aaagtttaag
     1021 taatttagta gatttaattc acagtaaaag tgtccgaaat gttcacttct atcctctagc
     1081 aggtggtcct agtcacatac acgctaaata ccatgtgaat acactgattt atgaaatgtc
     1141 tagaaaattt catggagagt gtacatttat gaatgcaacg attgtgcaag aaaataaatt
     1201 gttagcagat ggtattttgc aatcaagata ttttgaaaat ttgaaaaata gttggaaaga
     1261 tttagatata gctgtagtcg gaattggtga ttttagcaat aaaggaaagc atcaatggtt
     1321 agacatgctt acagaggatg actttaaaga attgaccaaa gttaaaactg taggagaaat
     1381 ttgttgtcga ttttttgatt caaaaggtaa agaagtctat gaaaacttac aggaaagaac
     1441 gatagcaatc tctttggaag atttaaaaaa tattcctcaa agtttagctg ttgcttacgg
     1501 tgatacgaaa gtatcttcga ttctttctgt cttgcgtgct aatttagtaa atcatttgat
     1561 tacagacaaa aatacaattt taaaagtttt ggaagaagat ggggatttga cttttagaga
     1621 gattctaggt gagtgaaaat gatagactga ttcagtttat cgtttttctt tttagttgat
     1681 tgcacatttg tgcttatata aacaaaaata gtttatctgt tgtttttgga
//
LOCUS       DAVMAF010000149         1624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVMAF010000149 DAVMAF010000000
VERSION     DAVMAF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.150"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..239)
                     /locus_tag="VYO97_002081"
     CDS             complement(<1..239)
                     /locus_tag="VYO97_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754813.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATIEQFKLYGNR"
     gene            complement(270..584)
                     /locus_tag="VYO97_002082"
     CDS             complement(270..584)
                     /locus_tag="VYO97_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1754814.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(594..>1624)
                     /locus_tag="VYO97_002083"
     CDS             complement(594..>1624)
                     /locus_tag="VYO97_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1754815.1"
                     /translation="DMYNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNY
                     SDIFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFG
                     AAQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKVS
                     VFLKSDEMEMIRKAMDQMQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDKVNLL
                     EKMVEGLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVAIC
                     KDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDF
                     INIFEKIK"
BASE COUNT          529 a          303 c          215 g          577 t
ORIGIN      
        1 ttcctatttc cataaagttt aaattgttca atagttgcta aaatagaaaa tactatgagc
       61 ataatgggga aaataataat aggcgaggga ttaataaact gattcaaaag ccaataaata
      121 ttcccaaaaa agaagagagc tattgaataa cgtagaagaa gatatcgatt aaaaaaagta
      181 ttagttagag ccatctctcg acgttgttgt tcaatttttt gtcgttcttt tttatccata
      241 tcatttcctc cttatataac aacacatatt tagttaactt tcttataaag agctaacatt
      301 tcctttgcta cttctaataa tgtcatagtg gtcattaaat gatcttgagc atgtaccatg
      361 ataatttcaa ttttaatttc cactccactt gcgtattctt gcaagagttt ggtttgtgca
      421 tgatgcgctt caagaattat ctcatttgat tgatttaatt tactttctgc atcatcaaaa
      481 ctaccttctc tcatttttgc aaatgcttca tgtatttctg accttgcatt tcccgaatgc
      541 aggataattt caaatgctgc aacctgcagt tcctcttgat tcatatgaac ctcctatttt
      601 atcttctcaa atatgttgat aaaatcttca aagttattgc aagatattag ctgattttgc
      661 aattcatcat tctctgtcag agagactatc tttttagtca cagttgctaa accttcgttc
      721 ccatatattg atggagatag aagaaatact agctggacat gtgaactttg attatcccag
      781 agtaacgaat ctttacaaat tgcaaccgaa acctttccct ctgtaccaaa gggctgaata
      841 ggatgcggaa ctgcaatttt ttcagaaaaa acaactgaac ttaattcttc gcgctgttta
      901 attccataaa gtaaagattg ttcaaactca tttgattcac caacagataa accctcaacc
      961 atcttttcaa gtaaatttac cttgtctgat tcagtacata ttaaaaaatt ttctttacta
     1021 aaatactgtc taaagccgtt gttttcaaat ttgttaatct ttgatgattg tacataacta
     1081 gaaacttgca tctgatccat agcttttcta atcatttcca tctcatcact tttaagaaac
     1141 acactaactt taaaaactgg gatttgaaaa tatagatttg ataaatcaac agctgacact
     1201 ataaaatcta ttcctttaag tttttcttga ttcaattcat agtagcctat tacatcaaca
     1261 acttctactc gcttcccaaa ctccgtttcc aaacgatttc ttaacatttg ggctgcacca
     1321 aatcctgttg cacaaatagc aagaatatta aacttagtac tctctttgct acgttccata
     1381 gcagctaaaa agtgaagact tacatatgct acttcatcat ctgatattgt ccactccaag
     1441 aacttgtcca tatttgcaag aatttctcta gtcataaaga atatatcact ataattctgt
     1501 ttaatttcat ctactaaagg gttatttaag gtaatccggc tttctaaacg tacttgtagt
     1561 gtcattagat gagttatcaa tccttcaatt agttggaaat ctgaagaaaa gttatacata
     1621 tcat
//
LOCUS       DAVMAF010000150         1597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVMAF010000150 DAVMAF010000000
VERSION     DAVMAF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.151"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            6..692
                     /locus_tag="VYO97_002084"
     CDS             6..692
                     /locus_tag="VYO97_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1754816.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            693..>1597
                     /locus_tag="VYO97_002085"
     CDS             693..>1597
                     /locus_tag="VYO97_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1754817.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIA"
BASE COUNT          510 a          222 c          313 g          552 t
ORIGIN      
        1 tctgaatgga aactaaaaaa attaaaaatt taaaaggtca aataattgtt tcctgccaag
       61 cgttagaagg agagccactt tatactccaa atggaggagt gatgccgtta ttagcaaaag
      121 ccgcctttca agcaggcgct aaaggtattc gtgctaattc tgtccgcgat atttcagaaa
      181 taaaagaaga agttgactta ccaattatag gtatcataaa aagagattac gatggctttg
      241 aaccttttat ttcagcaact atgaaagaga ttgatgaatt agttagtgaa ggagtagaca
      301 ttttagcttt ggattgtact aatcgttcaa gaccaggtta tgataatatt actgacttta
      361 ttcatgatat taaagtcaag tatccaaacc aattattaat ggctgatatt tctacttttg
      421 aagaagggaa agtagctgct gaatcgggag tagattttgt gggtacgaca ttatctggtt
      481 atacaccata tagccctaaa aaagataatc ctgattttga gttagttgaa agattggtaa
      541 aagagcttga cgtaccagtt attgctgaag gaagaataag tactccggaa caagctagaa
      601 aaatgttaga tttaggtgct tacgcagtgg ttgttggagg agctatcact cgtcctttgg
      661 aaatagcgaa gaagtttatt gaggtagttt agatgaatca agaaatatta aaaaagagta
      721 agattcaatt gacagttgaa catattcaaa aatattggat tttatattta atgatgattc
      781 cagcaactgt tctactaatt ttatttacgt atggaccaat gtacggcatt attatggcct
      841 ttcaagactt tactgtcttc aagggatata ctggaagtcc atttgtcggt ttaaaacatt
      901 ttcaaagatt gttctcagat cctttgtttt atagattgtt caagaatacg ttcatggtag
      961 gggttttaga ctttttattc tctttcccag ctccgcttat ttttgcactc atattaaacg
     1021 aagtcagaaa agtaagattt aagagtgtag ttcaatctat tagttatctt cctcatttta
     1081 ttcccctagt ggttatggtt ggtattattt ttgaactatt tggttcatat ggtattatta
     1141 acagtttact ttcatcactt ggaatggagc caataagttt ctttacaaaa tctgaatggt
     1201 tcttgcctct ctacattggt tcaggagtat ggaaaacaat aggttggggt tctatcattt
     1261 atatgggagc attaaccaat atagattcaa ctttatatga agcagcagat atggacgggg
     1321 ctaatagatg gcacaaaatg tggcatgtaa ctctaccaag tttaagacct acagtagtta
     1381 ctttgtttat actaaatgct ggtggtatca tgcaagtagg atttgaaaag gtattcttga
     1441 tgtctagtcc tgcaacatat gaagtatctg atgtattatc tacttatgtt tatagacaag
     1501 gtattttaaa ttctgatttc agttactcag ctgcagtagg attatttaat aacatagtag
     1561 ctttgttatt tgttttatta gctaataaga ttgctaa
//
LOCUS       DAVMAF010000151         1588 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVMAF010000151 DAVMAF010000000
VERSION     DAVMAF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.152"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            24..1370
                     /gene="gdhA"
                     /locus_tag="VYO97_002086"
     CDS             24..1370
                     /gene="gdhA"
                     /locus_tag="VYO97_002086"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1754818.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGHLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          490 a          308 c          325 g          465 t
ORIGIN      
        1 tatgacaata aaaggagaac gatatgacat ctgctaaaga atatatccaa agcgtgtttg
       61 aaactgtgaa agctcgtaac gggcacgagg ctgaattcct ccaagctgtt gaagaatttt
      121 tcaacacttt agaacctgta tttgaaaaac accctgagta tatcgaagaa aatatcttgg
      181 cacgtattac tgagcctgag cgcgtggttt ctttccgtgt tccttgggtt gaccgtgatg
      241 gaaaaattca agtaaaccgt ggttaccgtg ttcaattcaa ctcagctgtt ggaccataca
      301 aaggtggact tcgtttccac ccaactgtaa accaagggat cttgaaattc ctcggatttg
      361 aacaaatctt taaaaatgtc ttgactggac ttcctatcgg tggaggtaaa ggtggatcag
      421 acttcgatcc taaaggtaaa acagatgctg aagtgatgcg cttctgccaa agcttcatga
      481 ctgaattgca aaaacacatc ggaccatcac ttgacgtacc tgctggtgat atcggtgttg
      541 gtggacgtga aattggttac ctttacggtc aatacaaacg tcttaaccaa tttgatgctg
      601 gtgtcttgac tggtaaacct cttggatttg gtggtagctt gattcgtcca gaagcaactg
      661 gttacggttt ggtttactat actgaagaaa tgctcaaagc taacggtaac agctttgctg
      721 gtaagaaagt cgttatttca ggttctggta acgttgctca atatgctctt caaaaagcaa
      781 ctgaactcgg tgcaactgtt atttctgttt ctgactcaaa tggttatgtc atcgatgaaa
      841 atggtatcga cttcgatctt ttggttgatg ttaaagaaaa acgtcgtgct cgtttgactg
      901 agtatgcagc tgaaaaagca actgcaacct accacgaagg tactgtatgg acttacgctg
      961 gaaactatga cattgctctt ccatgtgcca ctcaaaacga aatcaacggt gaagcagcta
     1021 aacgtttggt tgctcaaggc gttatctgtg tatctgaagg tgccaacatg ccgagcgacc
     1081 ttgatgccat caaagtttac aaagaaaatg gtatcttcta cggacctgca aaagctgcca
     1141 acgctggtgg tgtagccgtt tcagctcttg aaatgagcca aaacagtctt cgcctctcat
     1201 ggactcgtga agaagttgat ggccatctca aagacatcat gacaaacatc ttcaacacag
     1261 ctaaaacgac ttcagaaaca tacggtcttg ataaagacta ccttgcagga gctaacattg
     1321 ctgcctttga aaatgtagca aatgccatga ttgcacaagg tattgtttaa gatttattta
     1381 ctcaatcctc aatcactacg attggggatt tttagcttga attaaaaaat tggaaaatct
     1441 aattaaataa aatgaaatga aatgaaataa aaactgaaca aattgattgc ggaattcaaa
     1501 ccaatttcta acatacttct agaaattaat ttgacttttc taatcgattt gttcatatct
     1561 tatttcaatt tactatactt acttaacc
//
LOCUS       DAVMAF010000152         1519 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVMAF010000152 DAVMAF010000000
VERSION     DAVMAF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.153"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            65..805
                     /locus_tag="VYO97_002087"
     CDS             65..805
                     /locus_tag="VYO97_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754819.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            808..>1519
                     /locus_tag="VYO97_002088"
     CDS             808..>1519
                     /locus_tag="VYO97_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754820.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVII"
BASE COUNT          497 a          196 c          299 g          527 t
ORIGIN      
        1 atacactgat atataaattg gaaaatggaa cacttttcaa aattaagtga ttggaggcaa
       61 gaatatggat aaagattata tattaaaagt gaaagggctg tatcatcaat ttttactggg
      121 aaacaataaa acgttgcaag tgctgaaaaa tgtttctctt tctgcttcga gaggagaatt
      181 tataagtatt ctaggaatta gtggttctgg aaagtcaact ttattaaaat gtatttctag
      241 tttgcttgaa ccaacaagtg gggaagtaat tttaaatgga atcaacccct ataaaatcag
      301 aaatgcaaaa ttgtcaagta taagacgtaa cgaagtatct tttatatttc aagcatacaa
      361 tttaatacct tccctgccgg taatagaaaa tatagcactt cctttgcgat tatcacaaaa
      421 aaaattaact attaaaaatg tagaaaactt actcaaaaga atgaagttta atgctggctt
      481 aaacgatttt gttggaactc tgtctggtgg agaacaacag aaggttgcta tagctagagc
      541 ggttattgct gatagtgata taatatttgc tgatgagcca actggggctt tagacagcgt
      601 ttctcgtgaa gtaatttttg aattattgag agagttagta ggggcgggta agtgtgtaat
      661 tatggtaacg cacgatatag aattggcctc gaaaactgat cgtgcattaa tattgaaaga
      721 tggaaaaatt ttcaaagaac ttcatagacc tagcggggaa gagttgtata aaatcttaga
      781 ggtacaatca actacggagg aatagagatg ctcagattag tttactatca atttttacat
      841 aataagaaac aatggttagg cgtttcacca gtaatttttg tttcgagttt agttatggga
      901 ttggctgtta atggggtaat taatgttgag aataattcgc aagtatttgt cggacttcca
      961 gatcctaaac caatttttat gtttccgata gtattcggag gagtaacatt attctttgtg
     1021 ctctctaaca taataaatat gttggttgaa atttttagag atgattacga acttctggag
     1081 gtgttgggag caagtctcct tcaactatca tttcttgtgg ggggacagat ttttatcatt
     1141 tcctcgatta ttagttttat tgcatatcta tgctctatat ttgttacgtc aaattattat
     1201 tattttttac aatacttttt cggggaaaat atattacctg atatccaatt tcaaacgagt
     1261 gctgttggat gtatcattac agtagtgttg atttcatttt tagcctttct aagtggttgc
     1321 ttttatacgt tcaagaagat tagaaatagg aagagttcta aaataaggca tgttctttca
     1381 atagtaaaaa gaatactgct attggctggt tttagtgtta tctggttgct ttctttgcaa
     1441 caaattttcc aagatagcac aattttagcg aaggcccaga taatattcaa tattgtaatc
     1501 ttagatattg tgattattt
//
LOCUS       DAVMAF010000153         1475 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVMAF010000153 DAVMAF010000000
VERSION     DAVMAF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.154"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..1460)
                     /gene="pfbA"
                     /locus_tag="VYO97_002089"
     CDS             complement(<1..1460)
                     /gene="pfbA"
                     /locus_tag="VYO97_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1754821.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVS"
BASE COUNT          448 a          291 c          202 g          534 t
ORIGIN      
        1 gatacttttc ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga
       61 tcggcaatat taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct
      121 ttagtgtttt tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca
      181 ctctctcctt taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct
      241 gtaaaacgta cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta
      301 gaggggttct gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca
      361 gatttatcac ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca
      421 tcatcattca aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc
      481 ttacttatga tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa
      541 ttatcaacta atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc
      601 ttgaatgtta cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa
      661 gaatttataa gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata
      721 tcaatcgtac caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca
      781 tcatccgtaa ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca
      841 tttagaattg tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt
      901 tcttttacta aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct
      961 gcagcatcta ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca
     1021 ccataatctt ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt
     1081 ataacatctt ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt
     1141 ctgttgtcta attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt
     1201 aacgaattat ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc
     1261 tgattaacag ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta
     1321 gccatcggtg aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt
     1381 gaaattgcca atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca
     1441 ttaggaacaa aatatttcat cagaacctcc aattt
//
LOCUS       DAVMAF010000154         1473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVMAF010000154 DAVMAF010000000
VERSION     DAVMAF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.155"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            19..306
                     /locus_tag="VYO97_002090"
     CDS             19..306
                     /locus_tag="VYO97_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754822.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            447..659
                     /locus_tag="VYO97_002091"
     CDS             447..659
                     /locus_tag="VYO97_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754823.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDL
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            829..1353
                     /locus_tag="VYO97_002092"
     CDS             829..1353
                     /locus_tag="VYO97_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1754824.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
BASE COUNT          551 a          209 c          340 g          373 t
ORIGIN      
        1 ctcagaaagg agaatctgat gaataagtac aaaaagttga tagaattgat tgaaaataac
       61 ggtcttgaga tacaatctaa gaaatgttat gatccacaga gtgcttggca tggtgaggag
      121 ttatggattg ttgataagaa aaaacaaaat aaaatttttg atttatcagg taacggttac
      181 tgttttcatg acgctaaagt tgaggaagcc attgaagaag ttgagaagta tctattattg
      241 aaaaagatgg atacgtttga tgatttcaaa aaatgggtgg aaaagaatgc taagcctaaa
      301 aaatgatgct tagaaaggag taaagacatg tttatatggg attgggtatc aatcgccttt
      361 gggtggttgg tatttttgtt gttaatattt attattatgg ccgtaatcag cggaataatt
      421 aaaggtgtaa agaaaggaac agaaaaatgg aagaatggaa agaaagattt aaaaaagaat
      481 actacgaatt gaaagaacga ttccagaagt tagatatgat gattgggaaa tacgaaaaag
      541 ggcaactaga gtttgaatct aaatgtccga ttgatttgtt aaaaggtcag cgttcaacca
      601 tgtggaatta tttaagaatt ctagaacaac gtgcaaaaat tgaagaaatt aaactataaa
      661 aattaaccgc atcgaaatcg aggcggtttt cttatgttct aaccgtatgg aatcccgtac
      721 ggtttttata ttgtccaaac tgtgccgatg acattaaaag ctgtactgtt ccgtcgccgg
      781 acgtaaagcg agattatcga gtggcgacgt aatcgctgga ggacaattat gtcagaagaa
      841 atcaatgcaa ctgtatctac tgaatcaact gagactgtcg acactcaaga aaatgttgat
      901 acagtgcagg aagaaaagca cgaacgaact ttcactcgtg ctgaaatcgg taagatgcta
      961 tctgccgagc gctctaaatg ggaagctgag caagaagcca aggaaaacga agctaagaag
     1021 cttgctaaga tgaacgctga tgaaaaacag aaatatcagt tggatcagcg tgagcaagaa
     1081 ctagctgacc gtgaaaaggc tattgctcgt aaggaattga ccgcagaggc taaagcaatg
     1141 ctaagtgaac gtgacttacc tgttgagtta gtaaatgtag tcgatttgac aaacgcagag
     1201 acggtatctg aatctatcac ctctatccaa aaagcatggg aagagtcagt tcagaaggga
     1261 gtctctgaac gtatgaaagg tagtgcacct atcaaaaatg cacaaacagt ccagcaagaa
     1321 gtcacggaaa aatggcgtaa agacttcttg taataaaaga aaagaggaaa aataaatggc
     1381 atttgaagaa ttaaacacag cagaatcacg caagaaacat cttgggatta ttgaggatgt
     1441 acttgcagta aattcatatt caacaccact tgt
//
LOCUS       DAVMAF010000155         1465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVMAF010000155 DAVMAF010000000
VERSION     DAVMAF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.156"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>1465
                     /gene="pcpA"
                     /locus_tag="VYO97_002093"
     CDS             <1..>1465
                     /gene="pcpA"
                     /locus_tag="VYO97_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1754825.1"
                     /translation="AVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTV
                     EGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETG
                     RIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELP
                     ASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNL
                     FRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELASY
                     SFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELI
                     LPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVK
                     KDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGV
                     VGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGS"
BASE COUNT          558 a          202 c          249 g          456 t
ORIGIN      
        1 ctgcggttat tttagcagca tatgtcccta atgaaccaat cctagcagat actcctagtt
       61 cggaagtaat caaagagact aaagttggaa gtattattca acaaaataat atcaaatata
      121 aggttctaac tgtagaaggt aacataggaa ctgttcaagt gggtaatgga gttactcctg
      181 tagagtttga agctggtcaa gatggaaaac cattcacgat tcctacaaaa atcacagtag
      241 gtgataaagt atttaccgtt actgaagtag ctagtcaagc ttttagttat tatccagatg
      301 aaacaggtag aattgtctac tatcctagct ctattactat cccatcaagc ataaaaaaaa
      361 tacaaaaaaa aggcttccat ggaagtaaag ctaaaactat tatttttgac aaaggcagtc
      421 agctggagaa aattgaagat agagcttttg atttttctga attagaagag attgaattgc
      481 ctgcatctct agaatatatt ggaacaagtg cattttcttt tagtcaaaaa ttgaaaaagc
      541 taaccttttc ctcaagttca aaattagaat taatatcaca tgaggctttt gctaatttat
      601 caaatttaga gaaactaaca ttaccaaaat cggttaaaac attaggaagt aatctattta
      661 gactcactac tagcttaaaa catgttgatg ttgaagaagg aaatgaatcg tttgcctcag
      721 ttgatggtgt tttgttttca aaagataaaa ctcaattaat ttattatcca agtcaaaaaa
      781 atgacgaaag ttataaaacg cctaaggaga caaaagaact tgcatcatat tcgtttaata
      841 aaaattctta cttgaaaaaa ctcgaattga atgaaggttt agaaaaaatc ggtacttttg
      901 catttgcgga tgcgattaaa cttgaagaaa ttagcttacc aaatagttta gaaactattg
      961 aacgtttagc cttttacggt aatttagaat taaaagaact tatattacca gataatgtta
     1021 aaaattttgg taaacacgtt atgaacggtt taccaaaatt aaaaagttta acaattggta
     1081 ataatatcaa ctcattgccg tccttcttcc taagtggcgt cttagattca ttaaaggaaa
     1141 ttcatattaa gaataaaagt acagagtttt ctgtgaaaaa agatacattt gcaattcctg
     1201 aaactgttaa gttctatgta acatcagaac atataaaaga tgttcttaaa tcaaatttat
     1261 ctactagtaa tgatatcatt gttgaaaaag tagataatat aaaacaagaa actgatgtag
     1321 ctaaacctaa aaagaattct aatcagggag tagttggttg ggttaaagac aaaggtttat
     1381 ggtattactt aaacgaatca ggttctatgg ctactggttg ggttaaagac aaaggtttat
     1441 ggtattactt aaacgaatca ggttc
//
LOCUS       DAVMAF010000156         1409 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVMAF010000156 DAVMAF010000000
VERSION     DAVMAF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.157"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(457..1233)
                     /locus_tag="VYO97_002094"
     CDS             complement(457..1233)
                     /locus_tag="VYO97_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1754826.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
BASE COUNT          472 a          196 c          239 g          502 t
ORIGIN      
        1 ttaatttgac tttcctgata gagatgttca catcttattt caattcacta tattagagta
       61 aaattctcta caaaaagaag aatagcctat tttactattc ttctgagtga tttcaattcc
      121 tttggggaaa tatggagata ctttttaaat cctgacaaat ggttgtttct ttttctaaat
      181 cggtgatact gtatcggaga atgcgcgtga ggtcacaaag gctgcgatag agcttctatg
      241 gagaatttct ttttggagag attttttaaa ggaatgagac atccgctacc tccttggaag
      301 gtttttttgt gataagtata gcagtttaaa aattctagag caaggtaaaa tagaaaaggt
      361 ttggctaaaa ttttttagct aaaccttttg aaaagttaag tatgtattat agatgcttgt
      421 tatctgcaaa tagctagagc atttcaagga aataaactag aaataacaat taatatggtg
      481 gttcagaaat accttttgat aatcaataag atcttcagga tgaagtgcgt tgattccatc
      541 catttcatat ttccgattta ataaacgata tttgttttca ccaaattgat gaaaagggag
      601 tagttgaact tggtcgatat ttaatgagtt aaatagagta gcgaattttt ctgcatcctc
      661 taaactattg ttaaaattag gaataactgg gattcttaaa acgatagttt tattttgtga
      721 aaaagcataa tgaatgtttt taataatcat ttgattaaaa accccagtca cttttttatg
      781 ttttatagaa ttataatgtt ttaggtctgt gtagataaaa tccacatatt gaattaaatc
      841 aataaatttt tcatgatcaa caaaggcagt agtttcaatg gcagtgtgta tgtgatgttc
      901 tttagctgat tttaagatgg ctttagcaaa ttcaaactga gcaaatattt cacctcctga
      961 taaagttaaa cctccaccgg attcttcgta aaattctttg tcttttaata cctctgtaat
     1021 aatttcttct acagtctttt cttcaccgac taaggtgaat ttctctcgtt gagcatcttt
     1081 cattttttca ggtttcattc tttgagattc aggattagaa caccatggac agcgcagagg
     1141 acatcctttt aaaaaaacag ttgtacgaat acccgggccg tcatgaattg aaaagtgttg
     1201 aatattaaaa ataattcctt tagatatttc cataagagtt cctcctcttc ataagtctat
     1261 cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag gttaatttat tgttttttgc
     1321 tttttatttt ggtagaataa aacggaggtg taaaatgaaa agattggaac aaattattaa
     1381 attagtatca gaatatgaaa agatcgacg
//
LOCUS       DAVMAF010000157         1388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVMAF010000157 DAVMAF010000000
VERSION     DAVMAF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.158"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            19..264
                     /locus_tag="VYO97_002095"
     CDS             19..264
                     /locus_tag="VYO97_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754827.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKSDIFAETYEKTEE"
     gene            266..517
                     /locus_tag="VYO97_002096"
     CDS             266..517
                     /locus_tag="VYO97_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754828.1"
                     /translation="MLKKAKQLASQEFSRLAGREIKEEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGDYAEITHNGDKNETYVDVYAKVSNRAIKD"
     gene            744..1268
                     /locus_tag="VYO97_002097"
     CDS             744..1268
                     /locus_tag="VYO97_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1754829.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
BASE COUNT          498 a          233 c          327 g          330 t
ORIGIN      
        1 cccagaaagg attgaggaat gaaataccgt aaaaaaccag tagtggtcga ggctgtgcag
       61 tggaacggca ataaccataa agaggtaatt gactttgcag aaaataagat ttggtttgat
      121 gcacttggga atatatggat tgctacactt gaaggtgata tggtagctaa aaaaggggat
      181 tatattatca aaggtgtgca aggagaatat tatccatgca agtcggatat ttttgcagaa
      241 acatacgaaa aaacggagga gtaaaatgtt aaaaaaagca aaacaattgg catcgcaaga
      301 attttcacgc ttggcaggtc gtgaaatcaa agaagaagac tgctttgtag tttggtttag
      361 caagaccctg caaaactgga aagctcttgt tagtacgaac gcaattacat caagcgaacc
      421 ttgtggagat tatgcagaaa ttacgcataa cggagataag aatgagactt atgtggatgt
      481 ttacgccaag gtttcaaatc gtgccattaa agattaggag gtgatccaac atcttgactt
      541 gcaggaatag actgctataa attactgtaa attgctataa accgcatcga aatcgaggcg
      601 gttttcttat gccctaaccg tatggaatcc cgtacggttt ttatattgtc caaactgtac
      661 cgatgacaat aaaagctgtg ctgttccgtc gccggacgta aagcgagatt atcgagtggc
      721 gacgtaatcg ctggaggaca attatgtcag aagaaatcaa tgcaactgta tctactgaat
      781 caactgagac tgtcgacact caagaaaatg ttgatacagt gcaagaagaa aagcacgaac
      841 gaactttcac tcgtgctgaa atcggtaaga tgctatctgc cgaacgctct aaatgggaag
      901 ctgagcaaga agccaaggaa aacgaagcta agaaacttgc caagatgaac gctgacgaga
      961 aacagaaata tcagttggat cagcgtgagc aagaactagc tgaccgtgaa aaagctattg
     1021 ctcgcaagga attgaccgca gaagctaaag caatgttaag tgaacgtgac ttacctgttg
     1081 agttagtaaa tgtagttgat ttgacaagcg cagagacggt atctgaatct attacctcta
     1141 tccaaaaagc atgggaagag tcagttcaga agggagtttc tgaacgtatg aaaggtagtg
     1201 cacctatcaa aaatgcacaa acagtccagc aagaagtcac ggaaaaatgg cgtaaagact
     1261 tcttgtaata aaagaaaaga ggaaaaataa atggcatttg aagaattaaa cacagcagaa
     1321 tcacgcaaga aacatcttgg gattattgag gatgtgcttg cggtaaattc atattcaaca
     1381 ccacttgt
//
LOCUS       DAVMAF010000158         1380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVMAF010000158 DAVMAF010000000
VERSION     DAVMAF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.159"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..215
                     /locus_tag="VYO97_002098"
     CDS             <1..215
                     /locus_tag="VYO97_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEV1754830.1"
                     /translation="KRLLVNFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWL
                     YRGLVVLAFLGILFVLWKLARLLRGK"
     gene            complement(288..1380)
                     /locus_tag="VYO97_002099"
                     /pseudo
     CDS             complement(288..1380)
                     /locus_tag="VYO97_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          418 a          234 c          304 g          424 t
ORIGIN      
        1 ataaacgctt attagtgaat tttgaacgag tcgctgttta tcaaaaatca gatccacaaa
       61 cagctgcagt tgcgagggtt gcttttacga aagaaggaca atctgtatcg cgtgttgcaa
      121 cctctcaatg gttgtaccgt gggctagtgg tactggcatt tctgggaatc ctgtttgttt
      181 tgtggaagct agcacgttta ctacgaggga aataaaaaga aatgaaagga aagctaaggc
      241 tgttcctttt tccggctctt tgtcaactgt agtgggttga aaaaaagcta agctcgagaa
      301 aggacaaatt ttgtcctttc ttttttgata ttcagagcga taaaaatccg ttttttgaag
      361 ttttcaaagt ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc
      421 gcttccagtt tggcattaga atagtgtagt tgaagggcgt tgataatctt ttctttatct
      481 ttgaggaagg ttttaaagac agtctgaaaa ataggatgaa cctgcttaag attgtcctca
      541 ataagtccga aaaatttctc tggttcctta ttctggaagt gaaaaagcaa gagctgatag
      601 agttgatagt ggtgtttcaa gtcttccgaa tagctcaaaa gcttgtttaa aatctcttta
      661 ttggttaagt gcatacgaaa aataggacga taaaatcgct tatcactcag tttacggcta
      721 tcctgttgaa tgagtttcca gtagcgcttg atagccttgt attcatggga ttttcgatga
      781 aactgattca tgatttgaac acgcacacga ctcatagcac ggctaagatg ttgtacaatg
      841 tgaaagcgat caagaacgat tttagcacac gggaaaagtt gtctagctaa gtcatagtaa
      901 ggactaaaca tatccatagt aatgattttg acgcgacatc ggacggctct atcatattta
      961 agaaagtgat ctcggatgac agcttgtgtt ctaccttcaa gaacagtgat gatattgaga
     1021 ttgttaaaat cttgcgcaat aaagctcatc tccatctccc gattgaaaca gtcactcccc
     1081 ggactgtttc aacgtcccag gacataatct caggaagacg cgaaaaatca tgctcaaagt
     1141 gagaatcatt gagcttgcga atgacagttg aagttgaaat ggccagctga tgagcaatat
     1201 cggtcataga aatcttctca atcaactttt gcgcaatttt ttggttggta atacgaggaa
     1261 tttgatgatt cttcttgacg atagaagttt cagcgaccat catttttgaa cagtgatagc
     1321 acttgaaacg gcgttttcta aggagaattc tagtaggtat accagtcgtt tcaagataag
//
LOCUS       DAVMAF010000159         1307 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVMAF010000159 DAVMAF010000000
VERSION     DAVMAF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.160"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(471..1259)
                     /locus_tag="VYO97_002100"
     CDS             complement(471..1259)
                     /locus_tag="VYO97_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754831.1"
                     /translation="MVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREISLLLATII
                     VYFFTHRILPFSVFTFMFSYILLFVQSYLGGNTAWIGNRRLIIDDEFEKILLSKSYIK
                     EISSARYSEYLTCEYKNLTPIILLAIFENLLDSYLLQNQSDVDLDIFYKVLPLLYKEK
                     YTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAV
                     ASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          464 a          233 c          177 g          433 t
ORIGIN      
        1 tatagaataa cggtatcgcc aatacaaagg ttaagatggc atgagaggga gttcctaaga
       61 tgtccaattt tataaatact gggctgacac aaaatatcgt gcttataagc agtaaaatta
      121 ttaaaattgt gtcaaaaaaa taacgcttat ttttcattag tctactttcc attgttaaat
      181 aatactactg ctaacaccaa gccaacttag cacattgcat cacctaatcc tgcgctactt
      241 gtccataatc ctttaatgtt tctactaaaa ttattccaat ctcccccatc aagatagctt
      301 atttatccta gacaacgcct actgcaacag tataaataag attcatcagc catcttttct
      361 ggcaatcata tgggtagact aatgatattt ctagttactc aaaaatatct agctatacta
      421 gagttatttc taatccctag gacacttgtt aatccaaatg taacaaattt tcacccctta
      481 ataggactct ttctggaaaa aatataaggt ctatctttat aaaaaaatct atcatattgg
      541 gatggcacgc atctggaatt taaacaagct ataaattcat ttataactac tatttgttta
      601 gaggcaacag catcttgtat accgccctcg aaagaaccat cttgtaattc cgaaatattt
      661 ttgtttaaat attgttttga caaatctctt aatgcttcat tatcgtaaat gattcccaaa
      721 aatccaacct tatatagtaa ataatttaaa cttacaaagt aattgaatcc catagtatat
      781 ttttctttat aaagcaaagg taaaacttta taaaaaatat ctaagtcaac atcactctga
      841 ttctgaagca agtaggagtc aagcaaattt tcaaaaatag ctaacaatat gattggtgta
      901 aggtttttgt attcacatgt caagtattcc gagtatcgtg cagatgatat ttcttttata
      961 taactttttg aaagcaagat tttttcaaat tcatcatcta ttattaatct tctatttcca
     1021 atccaagcgg tattaccacc caaataagat tgtacaaata gcaatatata agaaaacatg
     1081 aacgtaaata ctgaaaaagg tagaattcta tgcgtaaaaa aatagacgat aatggtagcc
     1141 aggagtaaag atatctctcg tgacaatcga attcttttca gcatcttttt taacagtccg
     1201 taaggttgtc cttggcaata ttcttgaact aaattaatcg gaatgacatc tctcaccatt
     1261 tctggaaagt agagtaagtt tataggttga aatctccagc tcccatc
//
LOCUS       DAVMAF010000160         1300 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVMAF010000160 DAVMAF010000000
VERSION     DAVMAF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.161"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>1300
                     /locus_tag="VYO97_002101"
     CDS             <1..>1300
                     /locus_tag="VYO97_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEV1754832.1"
                     /translation="IDNMNDWFIDATEDHVYIAERASEVEEIKNSKHRAFDNLKRSHL
                     RNTILPLLNIDKAHLYLISNYNAIAFGSAERLGKKSLEDIKDIVNKAADGYRNYYDFW
                     YRLASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGRYNTDKVYTPLREFFGPMDKYYN
                     YNGTGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISVYTHETTHVNDRAIYLGGFGHRE
                     GTDAEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRKNDGNQWYITDPKTLKTREDINR
                     YMKGYNDTLTLLDEIEAESVISQQNKDLNSAWFKKIDREYRDNNKLNQWDKIRNLSQE
                     EKNELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISYNDQSPYVGVRMMTGIYGGNTSK
                     GAPGAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQSKTDG"
BASE COUNT          467 a          189 c          248 g          396 t
ORIGIN      
        1 atatagacaa tatgaacgat tggtttattg atgctacaga agaccatgtc tacatcgcag
       61 aacgcgcttc tgaggtcgaa gaaattaaaa attctaaaca tcgtgcattc gataatttaa
      121 aacgaagtca ccttagaaat actatactcc cactactgaa tattgataaa gcacatcttt
      181 atttaatttc aaattataat gcaattgcct ttggtagtgc agagcgatta ggtaaaaaat
      241 cattagaaga tattaaagat atcgttaaca aagctgcaga tggttataga aactattatg
      301 atttctggta tcgtctagcg tctgataacg ttaaacaacg actactaaga gatgctgtta
      361 ttcctatttg ggaaggttat aacgctcctg gtggatgggt tgaaaaatat ggccgctata
      421 ataccgacaa agtatatact cctcttagag aattctttgg tcctatggat aagtattata
      481 attataatgg aacaggagct tatgctgcta tatatcctaa ctctgatgat attagaactg
      541 atgtaaaata tgttcattta gaaatggttg gtgaatacgg tatttcagtt tacacacatg
      601 aaacaacaca cgtcaacgac cgtgcgattt acttaggtgg ctttggacac cgtgaaggta
      661 ctgatgctga agcatatgct cagggtatgc tacaaactcc tgttactggt agtggatttg
      721 atgagtttgg ttctttaggt attaatatgg tatttaaacg caaaaatgat gggaatcagt
      781 ggtatattac agatccaaaa actctaaaaa cacgagaaga tattaataga tatatgaagg
      841 gttataatga cactttaact cttcttgatg aaattgaggc tgaatctgtg atttctcaac
      901 aaaataaaga tttaaatagt gcatggttca aaaaaataga tagagaatac cgtgataaca
      961 ataaattaaa tcaatgggat aaaattcgaa atctaagtca agaagagaaa aatgaattaa
     1021 atattcaatc tgttaatgat ttagttgatc aacaattaat gactaatcgc aatccaggta
     1081 atggtatcta taaacccgaa gcaattagct ataacgatca atcaccttat gtaggtgtta
     1141 gaatgatgac cggtatctac ggaggtaata ctagtaaagg tgctcctgga gctgtttcat
     1201 tcaaacataa tgcttttaga ttatggggtt actacggata cgaaaatggg ttcttaggtt
     1261 atgcttcaaa taaatataaa caacaatcta aaacagatgg
//
LOCUS       DAVMAF010000161         1293 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVMAF010000161 DAVMAF010000000
VERSION     DAVMAF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.162"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            26..810
                     /locus_tag="VYO97_002102"
                     /pseudo
     CDS             26..810
                     /locus_tag="VYO97_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(845..1054)
                     /locus_tag="VYO97_002103"
                     /pseudo
     CDS             complement(845..1054)
                     /locus_tag="VYO97_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1220..1293)
                     /locus_tag="VYO97_002104"
     tRNA            complement(1220..1293)
                     /locus_tag="VYO97_002104"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1257..1259),aa:Asn,seq:gtt)
BASE COUNT          430 a          264 c          212 g          387 t
ORIGIN      
        1 gattttctag tataaaaaaa ttcaaactag aattcttctt agaaaaggcc gttttaagtg
       61 ctatcattgc tcaaaaatgg aggtcgctga gacttctatc gtcaagaaga atcatcaaat
      121 tccttgtatc atcaaccaaa agattgatca gaagctaatt gaaaagactt ctatgaccga
      181 cattgatcat cagttgtcta tttcaacttc aactgtcatt cgcaagatca atgattttca
      241 ctttgagcat gatttttcgc gtcttcctga gattatgtcc taggacgttg aaacagtctg
      301 gggagtgact gtttcaatcg ggagatagag atgagcttta ttgctcagga ctttgataag
      361 ctcaatatca taactgttct tgagagcaga acacaagcca tcatccgaaa tcccatgaat
      421 acaaggctat caagcgatac tgaaagctca ttcaacaaga tagtcagaaa ctgagtgata
      481 aacgatttta tcgccctact tttcgtatgc acttgactaa taaagaaatt ctagataaac
      541 ttttgagcta ttccgaagac ttgaaacacc actataatct ctatcagctc ttgcttttcc
      601 accctgttta aaaccttcct caaagataaa gagaaaatcg tcaacgccct tcaattacct
      661 tattccaacg ccaaactgga agccaccaat aatctcatca aacttatcaa gcacaatgcc
      721 tttggtttta ggaactttga aaacttcaaa aaagaaagga cgaaatttgt cctttctaga
      781 tcttcgcttt cttcaaccca ctacagttga caaagaaccg aaaaatgtct cgcctctaat
      841 atagtcaatt gaaacaagag caggacaaaa gagcctcgta aaaggtatgg caacgtggta
      901 ataccttttt gaggttcttt ttgatatgag cccatgtttt ctcaatagga ttgtactcag
      961 gtgagtaggg aagaagaggt aaaagtttat gcccaaactc ttcacataaa agttctagct
     1021 tacccattct atggaatctt gcattgtcca taatatagta gattgaaact agaatagtac
     1081 acctctactt ctaaaacatt gttagaattc gattttactg tcctgatcga tttgtcctat
     1141 tcttatttca ttttgctata acacgaaata gattattatt acataacaaa aaagaggtct
     1201 aaacctctta actaaattac tccgccagta ggactcgaac ctacgacatc atgattaaca
     1261 gtcatgcgct actaccaact gagctatggc gga
//
LOCUS       DAVMAF010000162         1270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVMAF010000162 DAVMAF010000000
VERSION     DAVMAF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.163"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>1270
                     /locus_tag="VYO97_002105"
     CDS             join(<1..424,424..>1270)
                     /locus_tag="VYO97_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1754833.1"
                     /translation="DKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIV
                     KKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKNGYTIVE
                     KTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRSPRARKA
                     ETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNY
                     AYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAENLIQRQ
                     FEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQVKSMLE
                     QAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGIL
                     KSEMFYGYEKNFRSLENLEQAIVDYIDYYNN"
BASE COUNT          453 a          218 c          280 g          319 t
ORIGIN      
        1 gataaagttc agatctatga actaagaaag caaggacaaa gcttcaaaca gctttcaaaa
       61 agatttggtg tggatgtttc tggtctaaag tacatggtga aattaattga ccgttatggc
      121 atagaaatcg ttaaaaaagg gaagaatcgt cactattctt ctaaattgaa acaagaaatg
      181 atggataaag ccttactgga aggttgttca caaagaagta ttagtcttga ttatgccctc
      241 ccaaaccaag gaatgctttc attttggccg gcacaataca agaaaaacgg gtatactatt
      301 gttgagaaaa caagagggag accagctaaa atgggacgta aacggaagaa gacttgggaa
      361 gaaatgacag aactagaacg actccaggag gagaatgaac gcttacggac tgaggtggcc
      421 tacctaaaaa agttaaaaga gttagaggaa agggacgaag ccctagagcg agaaaggcag
      481 agacagttag agaaatggtt tcaggaggat ttcgactaga ttttcttctt gaaacagcac
      541 gtttagctcg ctcgacttac tattatcagt tgaaacaact ggatggggtt gataaagata
      601 aagagattaa aaccgaaatt cagggcattg ataatgaaca taaaggcaac tatgcctatc
      661 gtcgaatcca tttagaacta aggaatcgtg gttttgtggt caatcataag aaagttcaac
      721 ggttgatgag aattcttggt ttaacggctc gaattcgccg taaacggaag tattcttcct
      781 accaaggaga gattggcaag aaagcagaga atctcattca acgccagttc gaagcatcga
      841 ggccaatgga aaagtgctat acagatgtga cagagtttgc cattccaaat agcacacaga
      901 aactctattt atcaccagtt ttagatggtt ttaacagtga aatcattgct tatcatcttt
      961 ctacttcgcc aaacttagaa caagtgaaga gtatgttaga acaggccttt acagagaaat
     1021 actatgagaa tacgattctc catagtgacc aaggctggca ataccaacac gattcttatc
     1081 atcggttcct agagagtaag gaaattcaag catccatgtc acgcaagggc aacagcccag
     1141 acaacggcat gatggaatct ttctttggca ttttaaaatc cgaaatgttt tatggctatg
     1201 agaagaactt tagatcttta gaaaaccttg aacaagctat tgtggactac attgattact
     1261 acaacaacaa
//
LOCUS       DAVMAF010000163         1163 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVMAF010000163 DAVMAF010000000
VERSION     DAVMAF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.164"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            94..>1163
                     /locus_tag="VYO97_002106"
     CDS             94..>1163
                     /locus_tag="VYO97_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV1754834.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCE"
BASE COUNT          427 a          138 c          216 g          382 t
ORIGIN      
        1 gaaaattcct tagttttgtt tgttggtgca ggaatatcag ctaattctaa tctaccaact
       61 tggggagaat tgattcaatc attaaaaaaa gaattgaaca taccagaaga aagaacagat
      121 agtccactga gaattgctca gtattattat gatacttttg ggaaaaatca gtacacaaag
      181 aagatagaag aaattttctt taagaaggga ttgagtaaac caaatgagtt acataaatta
      241 atagaaaaaa ttgcaccaaa acatattatt acgacaaact atgattcact tctggagagt
      301 caatttgaaa gtggcttgtt aaagtacaat gtagtcgctg aggataaaga catcccttat
      361 acgagttcgg aaagatactt gattaaaatg catggtgact tcagtaagaa aaatattgtt
      421 ttgaaagaag atgattattt agactatcat ttgaacttcc caatgatttc tactttaatc
      481 cagtcattga taatgaatca tactctattg tttgtggggt attctctaag tgattcgact
      541 tttaattcaa tttttagaat gattcaaaat acatttaaat tggatgctaa aaatgcttat
      601 ttttatactc ccgaggaacc gtctatgatc attcgagagt attataagaa gcaaggaatt
      661 ttcattatct ctaatgaaga gaatctaggt caggagacat cagaaaaaca gaacaaattg
      721 tattgcagga caaaagattt tttagaggtt ctatcagaaa accgaagtca agatgtaaat
      781 aatgctgatg atttatggaa tcaactggct tttttagata gacttagttt tattgatgcg
      841 aaagattttt ctagatattc tgatttaaag aaaagggctt taaactggga tgatgagtat
      901 tgctggtttg gaaatgatca attaagattt gaaattgatg gtcatgaaga attacgaatt
      961 atggtatcta aaaaatcttt attaaatcgc tttctagata tggagattgg tgagcctagg
     1021 gatttaaagg ggaatcgatt cttaagtaag gcttttaagt tgtatgaaga aaaacaatat
     1081 tctttagcaa aagcaaaatt tagagaacta gctaatatag catttcgtca gaaagactat
     1141 tttaatttct tagtttgtga att
//
LOCUS       DAVMAF010000164         1115 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVMAF010000164 DAVMAF010000000
VERSION     DAVMAF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.165"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(1..702)
                     /locus_tag="VYO97_002107"
     CDS             complement(1..702)
                     /locus_tag="VYO97_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1754835.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNRLLIYLNTSGIKILEIQNGIGNLDEERQLNKIKSTKVSCYLSLKLNENFEHLKLME
                     EMHNIAGVIYVEEIR"
     gene            complement(704..>1115)
                     /locus_tag="VYO97_002108"
     CDS             complement(704..>1115)
                     /locus_tag="VYO97_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1754836.1"
                     /translation="TVIILLIAYTIQKLPFSYRMIRAVFFSIDNDMEEAARSMGASSF
                     YTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFLYHPLFQPLGIVIKQSTDETATL
                     NAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
BASE COUNT          399 a          211 c          155 g          350 t
ORIGIN      
        1 ttacctaatc tcttcaacat aaataacacc agcaatatta tgcatttctt ccattaactt
       61 taaatgttca aagttttcgt ttaattttaa tgataaatag caggacactt ttgttgactt
      121 aattttattt aattgacgtt cttcatcaag atttcctata ccattttgaa tttctaaaat
      181 cttaataccc gatgtattta aatatataag taaacgatta taaccttcta aagaactaaa
      241 aaccaagtag taatcagcaa ttttagtttt cgagtttaaa taatctttaa gaggagtaaa
      301 tagagttaaa actgctatga gtacaactcc ggctaaaata gcaccttcat aaaaaccaat
      361 gcctatagct aaaccaatag ttgctgaaga ccataatcca gctgctgttg ttaaaccttt
      421 aactttttga ttagttacca tgattgttcc tgcccctagg aaacccaagc cactaattac
      481 ttgggctccc attcgactag gatcaccgct accataacga ctagtgatga actgatttgt
      541 catcattaca acacaagttc ccaaacaaac tagtaagtag gttctaatcc ctgctgcttg
      601 gtttttgact cctcgctcat agccaacaat gccaccgaaa agaatcgcta aaaagcacct
      661 aagaagtatt tcccaaatac tcagttcgta tgaaagattc atattatctc ttacctcgtt
      721 taccttggaa taggcttgat aaataaagag ctgcactaga cataatcatt aaaattacag
      781 aataaacaaa catcattgcc tgtgcattta aagttgctgt ttcatcagta gactgtttaa
      841 taacgattcc caatggttgg aaaagtggat ggtacaagaa tacagataag tcatagtcag
      901 ataataaaga attaaagttt aacacaacta ctgataatac gactggtaaa atatacggta
      961 taataactcg caccatagtg tagaaactag atgcacccat actacgtgct gcttcttcca
     1021 tatcattatc tatactgaaa aatacagccc gaatcattct ataagaaaat ggtaattttt
     1081 gaatagtata tgcaataagt aaaataatta ctgtt
//
LOCUS       DAVMAF010000165         1109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVMAF010000165 DAVMAF010000000
VERSION     DAVMAF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.166"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..657
                     /locus_tag="VYO97_002109"
     CDS             <1..657
                     /locus_tag="VYO97_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754837.1"
                     /translation="KTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPE
                     SITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRV
                     WIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARY
                     ADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS
                     "
BASE COUNT          457 a          183 c          177 g          292 t
ORIGIN      
        1 aaaaccttgt ctcgacttat aaagccatta gagggagaag tattgcttga taataaatca
       61 attaattctt ataaagaaaa agatttagca aaacacatag ctatattacc tcaatctcca
      121 ataatccctg aatcaataac agtagctgat cttgtaagcc gtggtcgttt cccctacaga
      181 aagcctttta agagtcttgg aaaagatgac cttgaaataa taaacagatc aatggttaag
      241 gccaatgttg aagatctagc aaataaccta gttgaagaac tttctggggg tcaaaggcaa
      301 agagtatgga tagctctagc cctagcccaa gatacaagta tcctactttt agatgagcca
      361 actacttact tggatatctc atatcaaata gaactattag acctcttgac tgatctaaac
      421 caaaaatata agacaaccat ttgcatgatt ttgcacgata taaatctaac agcaagatac
      481 gctgattacc tatttgcaat taaagaaggt aaacttgttg cagagggaaa gcctgaagat
      541 atactaaatg ataaactagt taaagatatc tttaatcttg aagcaaaaat tatacgtgac
      601 cctatttcca attcgcctct aatgattcct attggcaagc accatgttaa ctcttaataa
      661 ttgaatattg gaattcattt aaaagtcgca acttttgaac tgaccctcaa aagttaggcc
      721 aaaaaactaa ctaaaggagg tcagtttttt aatggcaact acagcacaga atttaagata
      781 aaattagtaa aagaatatca tgaatgaaaa ataggatata gagaataaca gcaatattaa
      841 aaagatcagg actgataata aacaaaaaga aagtacaacg actagttcaa aagctaaaac
      901 ttcaagtaaa aagttattca agaaaatcta gaaaatactc atcctacaaa ggacgggtag
      961 gaaaaatata agacaacaaa ataaaaagaa atatttatta ccgaaaaaaa ggggaaatcg
     1021 agtcaagtac caacaggctc atctattcca aaaagatatt tactcgatgg aaaaactcct
     1081 agaattagtg ctacaaatat tgacaatgg
//
LOCUS       DAVMAF010000166         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVMAF010000166 DAVMAF010000000
VERSION     DAVMAF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.167"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(55..>1095)
                     /locus_tag="VYO97_002110"
     CDS             complement(55..>1095)
                     /locus_tag="VYO97_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754838.1"
                     /translation="GVLLYQNWDTVVEFAKTAWQGLCDFISGICQSIGEFFSGLWTKL
                     QEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQR
                     WADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWNDVTSALSKVASWFGDI
                     FGKAFDAVKNAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIEN
                     VVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEAV
                     VPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEINK
                     EHERAGQTLLKI"
BASE COUNT          305 a          299 c          202 g          289 t
ORIGIN      
        1 atttgggagg cttcacagcc accccattga ttgtcaattg tgccatttaa cctcctaaat
       61 cttgagcaag gtttgacctg ctcgttcatg ctccttgttg atttcttgga tggctacccg
      121 tccgaactca tggcctgcga tttggataac gatgtcgccg tcgccagaga atccaccttg
      181 tggactaaca ccagccatgg catttactac cgcactgctg actactcgtc caagtgtttg
      241 gataaatcct gtattttcaa gtggtacgac cgcctcttta ccagcttcac caatcatggc
      301 gattgttgga ctatcgacga taccaccacg ggcaagacga gggaggctaa ctgtacttac
      361 actaccaacc catcctagac caggtaagtt tctgacaacg cctaaaactc cattaatcat
      421 tccgatgaag ccattgacta cattttcaat cgttccaaga accgcattga ccgcactctt
      481 aaacgctcca cctactgcct cgccgaccat ctgaccagca ttaacgaaga tacttttgac
      541 agtatcccaa acgcctttaa agaagtctcc gatagagcta aaggcatttt taacagcgtc
      601 aaaagctttt ccgaatatat cgccaaacca gcttgctact ttggaaagtg cacttgtaac
      661 atcgttccat ctctcgccaa accaagaacc tagtttgcta aagatgtttg ttaagccagt
      721 ccatgctttt tggaacatgt cagtaaacca tgccccgata ttagccaacg cactagtcac
      781 atctgcccaa cgttgtccga accatgagcc gattggagtg aagatattaa cgatagcgtc
      841 ccatgcacct tggaatacac cagagaacca ctctccgatg ccagagaata tgtttacaat
      901 ggcgtcccat gcttgctgga atttctcgcc aaaccattga cctatcggct caaagatttc
      961 ttgtagtttc gtccatagac cgctgaaaaa ttcgccaatc gattgacaaa taccactgat
     1021 aaaatcacat agtccttgcc atgcagtttt agcaaactca acaacagtgt cccagttttg
     1081 atagagcaaa acacc
//
LOCUS       DAVMAF010000167         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVMAF010000167 DAVMAF010000000
VERSION     DAVMAF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.168"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(55..>1095)
                     /locus_tag="VYO97_002111"
     CDS             complement(55..>1095)
                     /locus_tag="VYO97_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754839.1"
                     /translation="GVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGLWTKL
                     QEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQR
                     WADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGSWFGERWNDVTSALSKVASWFGDI
                     FGKAFDAVKNAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIEN
                     VVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEAV
                     VPLENTGFIQTLGRVVSSAVVNAMAGVSPQSGFSGDGDIVIQIAGHEFGRVAIQEINK
                     EHERAGQTLLKI"
BASE COUNT          310 a          296 c          200 g          289 t
ORIGIN      
        1 atttgggagg cttcacagcc accccattga ttgtcaattg tgccatttaa cctcctaaat
       61 cttgagcaag gtttgacctg ctcgttcatg ttccttgttg atttcttgga tggctacccg
      121 tccaaactca tggcctgcga tttggataac gatgtcgccg tcgccagaga atccactttg
      181 tggactaaca ccagccatgg catttactac cgcactgctg actactcgtc caagtgtttg
      241 gataaatcct gtattttcaa gtggtacgac cgcctcttta ccagcttcac caatcatggc
      301 gattgttgga ctatcgacga taccaccacg ggcaagacga gggaggctaa ctgtgcttac
      361 actaccaacc catcctagac caggtaagtt tctgacaacg cctaaaactc cattaatcat
      421 tccgatgaag ccattgacta cattttcaat cgttccaaga accgcattaa ccgcactctt
      481 aaacgctcca cctactgcct cgccgaccat ctgaccagca ttaacgaaga tacttttgac
      541 agtatcccaa acgcctttaa agaagtctcc gatagagcta aaggcatttt taacagcgtc
      601 aaaagctttt ccgaatatat cgccaaacca gcttgctact ttggaaagtg cacttgtaac
      661 atcgttccat ctctcgccaa accaagaacc tagtttgcta aagatgtttg ttagaccagt
      721 ccatgctttt tggaatatgt cagtaaacca tgccccgata ttagccaaag cactagtcac
      781 atctgcccaa cgttgtccaa accatgagcc gattggagtg aagatattaa cgatagcgtc
      841 ccatgcacct tggaatacac cagagaacca ctctccgata ccagagaaga tgttgacaat
      901 ggcatcccat gcttgctgga acttctcgcc aaaccattga cctatcggct caaagatttc
      961 ttgtagtttt gtccatagac cactgaaaaa ttcgccaata gctcgacaaa taccactaat
     1021 aaaatcacat agtccttgcc atgcagtttt agcaaactca acaacagtgt cccagttttg
     1081 gtagagcaag acacc
//
LOCUS       DAVMAF010000168         1087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVMAF010000168 DAVMAF010000000
VERSION     DAVMAF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.169"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..978
                     /locus_tag="VYO97_002112"
     CDS             <1..978
                     /locus_tag="VYO97_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1754840.1"
                     /translation="FSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVAT
                     IAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNF
                     EAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGME
                     KEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANY
                     LTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLG
                     KINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            968..>1087
                     /locus_tag="VYO97_002113"
     CDS             968..>1087
                     /locus_tag="VYO97_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1754841.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFL"
BASE COUNT          427 a          154 c          182 g          324 t
ORIGIN      
        1 tttagcgctt gttcttctaa ttctgttaaa aatgaagaaa atacttctaa agagcatgcg
       61 cctgataaaa tagttttaga tcatgctttc ggtcaaacta tattagataa aaaacctgaa
      121 agagttgcaa ctattgcttg gggaaatcat gatgtagcat tagctttagg aatagttcct
      181 gttggatttt caaaagcaaa ttacggtgta agtgctgata aaggagtttt accatggaca
      241 gaagaaaaaa tcaaagaact aaatggtaaa gctaacctat ttgacgattt ggatggactt
      301 aactttgaag caatatcaaa ttctaaacca gatgttatct tagcaggtta ttctggtata
      361 actaaagaag attatgacac tctatcaaaa attgctcctg tagcagcata caaatctaaa
      421 ccttggcaaa ctttatggag agatatgatt aaaattgatt caaaagcctt aggtatggaa
      481 aaagaaggtg atgagttaat caaaaatact gaagctcgta tatccaaaga attagaaaaa
      541 catccagaaa tcaaaggaaa aatcaaagga aaaaaagtat tatttactat gattaatgct
      601 gcagatacat caaaattctg gatttatact agcaaagatc caagagcaaa ttatttaaca
      661 gatttaggtc tagttttccc tgaatcatta aaagaatttg agagtgaaga tagttttgca
      721 aaggaaattt ctgcagaaga agcaaataag ataaatgatg ctgatgtaat cataacttat
      781 ggtgatgata aaactcttga agctttacaa aaagatcctc ttttaggtaa aataaatgca
      841 attaaaaatg gtgccgttgc tgtaattcca gataatacac cgttagcagc ctcatgcact
      901 ccaacaccac tttcaataaa ctatactatt gaagaatacc taaatctttt aggaaatgca
      961 tgcaaaaatg cgaaataaaa aacaaataaa cctaggcata atttttgtaa tctgcctagg
     1021 tcttcttatt acaatatttt tgtcattaaa gcttggaaca aaagaaatta atatcagaga
     1081 tttttta
//
LOCUS       DAVMAF010000169         1075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVMAF010000169 DAVMAF010000000
VERSION     DAVMAF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.170"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            81..419
                     /locus_tag="VYO97_002114"
     CDS             81..419
                     /locus_tag="VYO97_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754842.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETIRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            394..909
                     /locus_tag="VYO97_002115"
     CDS             394..909
                     /locus_tag="VYO97_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1754843.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            906..>1075
                     /locus_tag="VYO97_002116"
     CDS             906..>1075
                     /locus_tag="VYO97_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754844.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIR"
BASE COUNT          351 a          195 c          262 g          267 t
ORIGIN      
        1 agtagtctac ggagaaaacg gcctgtctac gggcctttta cagcgtatac gtatgcatag
       61 actagcaagg gtggcaggcc atgtttttga agcagagtag actgaaacct tatccaatgc
      121 gacggtttga aaagactgtc actgaggaag gtgtcgcaaa agaaggatat gtcaaggaag
      181 ctgagacaat ccgtcttgag ttgtggccag ctagtagtaa actacagtct gaattgtatg
      241 gcgagcgtgt caatgatatt ttgaacgcaa atgccaacaa gtcagctaca atcaaagtga
      301 aagatggtgt gtgtatcgat agccagacag aagtgactca cagggttatt tctaaaaagg
      361 tctacacaca tcatcaagtt ttggagttag agcgtgtcag ggctactagg ggcagatagg
      421 ctcatagcta aatgtagacg attggctagt aaaaaaactg gcgaggatat cgtcttacgt
      481 gcggtacaca atgctactat aaaggttgtc caagcagatg caagaagact cgcaccagcg
      541 agagatggag agcttataac tagtatcaaa actagggcaa aaatggacgg agataaggct
      601 ataggcgagg tttacaccaa cctaaaatac gctccttacg ttgagtttgg aacgggacca
      661 aaaggacaag ctagccattc tggtatctct ccagaggtca gcgtaactta caagtctagt
      721 ccgtggtatg tgcatgaaga ccaaatcaat gtaggaccgt accactttca aaagattggg
      781 gagttctaca agatgtatgg tcaacctgct cagccttatc tttatccagc tttgagagac
      841 aatcaagagc gtgtgtctaa gaatatttcg aattatgtcc gtagaaagat aagagaacaa
      901 ataaaatgat taatatcaag cctgttattt ataaagaatt gcaaaaggtc gcagataatg
      961 tgactgatac ttatcctagc gattgggaga ctttcccagt cgttattttt ttagaagaac
     1021 aaaacaagcc gggtgattgg tttgatgacc aggaacaaaa atcatctatc cgcta
//
LOCUS       DAVMAF010000170         1034 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVMAF010000170 DAVMAF010000000
VERSION     DAVMAF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.171"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..578
                     /locus_tag="VYO97_002117"
     CDS             <1..578
                     /locus_tag="VYO97_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754845.1"
                     /translation="SKIPSGLYKIDTGKVLFDGININQIDKKILSQNLGVVPQDSFLL
                     NRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRI
                     ALARALINNPSIVILDEATSALDTINEERITKYIKSQGCTQIIVAHRLSTIKDADIIF
                     VMKGGKIVESGNHKYLMELGGEYYSLYTKRK"
     gene            591..761
                     /locus_tag="VYO97_002118"
     CDS             591..761
                     /locus_tag="VYO97_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754846.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          378 a          127 c          196 g          333 t
ORIGIN      
        1 tatcaaaaat accatcagga ttatataaga ttgatacagg aaaagtttta tttgatggta
       61 taaatattaa tcaaatagat aaaaaaatat taagtcaaaa tttaggagta gttccacagg
      121 attcattttt attgaaccga agtattcttg ataatataac tttaaagaac gaagttactt
      181 cacaaaagat agaggaagtt tgtaaagcag ttcaaatcta tgatgaaatc atggctatgc
      241 cgatgaaatt taatactatc atctcagaga tgggttcaaa tatttcaggt ggtcaaagac
      301 aacggatagc actggcacgt gcattaataa ataatcctag tattgtaatt ttagatgaag
      361 caactagtgc tttagacact attaatgagg aaagaataac aaagtatata aaaagtcagg
      421 gctgtactca aataattgta gctcatagat tgtcaacgat taaggatgcg gatattattt
      481 ttgtaatgaa aggtggtaag attgttgagt caggaaatca taagtactta atggagcttg
      541 gtggagagta ctacagctta tatacaaaaa ggaaatgagg tgtaaagaaa atgaagaaag
      601 aaaatgaata tgtaatttta acaacagcct cactaggggt gatgattgga atagtgtttg
      661 caattttttt agattttcca gttgaatatg gtatttcttt aggcttgttg aatggaatag
      721 tattgggttc gctgattgtt tacaaaaaca ataagaatta agcataattt tttgctgtaa
      781 actaaggagt agagatggct atagttgaaa ttataaatct aacaaaaagc tttaaagata
      841 ttgaagttat tcataacact taaataatag agcaactaca gtagtagctt aaaaacatga
      901 ttaaatcgct attcttagga gtagcggttt ttctttttgt ttaatactct ttgaaaatct
      961 cttcaaacca cgtcagcttt gccttgccgt actcaagtac agcctgcggc tcgcttccta
     1021 gtttgctctt tgat
//
LOCUS       DAVMAF010000171         1013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVMAF010000171 DAVMAF010000000
VERSION     DAVMAF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.172"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            77..565
                     /locus_tag="VYO97_002119"
     CDS             77..565
                     /locus_tag="VYO97_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754847.1"
                     /translation="MGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKL
                     ALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRD
                     IFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNR
                     SK"
     gene            581..>1013
                     /locus_tag="VYO97_002120"
     CDS             581..>1013
                     /locus_tag="VYO97_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1754848.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISASIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTIL"
BASE COUNT          344 a          129 c          178 g          362 t
ORIGIN      
        1 tcaagaaaga tttagatgtt tgatccaaga gttagatttg actcagtcta ttaataaaaa
       61 agtaaagacc tattctttgg gtacaaaaca aaaattagct ttgcttctaa ctctcgttac
      121 ggaacctgat atattgattt tagatgaacc gactaatggt ttagatattg aatcatcaca
      181 aatagtttta gcggttctaa aaaaattagc tttacatgaa aatgtgggaa ttttaatatc
      241 gagtcataaa ttagaagaca ttgaagaaat ttgtgagaga gttcttttct tggagaacgg
      301 gcttttgaca tttcaaaaag taggaaaaga tagtcataat ttcttgtttg agatagcttt
      361 ttcatcagct acagatagag acattttcat taccaaacaa gaatttgggg atattgttca
      421 ggaagaggga ctgagaatta ctatgtctgg gaatattcaa agtagtgagc tttttaaatt
      481 ttttaacgaa aactctatta aagtagttga ttttgaaact aaaaaagaga cgcttaaaga
      541 tatttaccta aatcgttcaa aataaaggaa ggttataatc atgaaattaa ataaattgaa
      601 ttttcttaag gaaaatataa gaaatttata ttcatcaggc gtaatatatc tcagtttgct
      661 tatctcgttt ataccgccga tattgcttac attctttatt ctaaagactc aagggacatc
      721 gcttggtatt aagcatattt caaactttta tgctatgctc ggtatgttaa tggctgttat
      781 acatgctaat cgagtcatta gtagagattt ttcccacaat acggtaagtt tgttttataa
      841 tcaacagaag aatcggatga tttacgtctt gtctaatttt ctatatgcca tctcagcttc
      901 cattatttat gctttgaatg gcattgtgtt actagtcata gtaagtaaat tgggtattcc
      961 aggtgattta ggattagatt ttatagtagc tattgtagtc aatacaattt tgt
//
LOCUS       DAVMAF010000172          964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVMAF010000172 DAVMAF010000000
VERSION     DAVMAF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.173"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..961)
                     /locus_tag="VYO97_002121"
     CDS             complement(<1..961)
                     /locus_tag="VYO97_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1754849.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLY"
BASE COUNT          344 a          170 c          128 g          322 t
ORIGIN      
        1 catataacgg aaaatctcta ttcacctcat ttggttcgat atcattcatt ttatttgact
       61 caattggatt aattgaatac aattttaaaa aaataggctt cgtaccaata atagaggata
      121 attgacctcc taattcacct agcgctgtaa aaataacacc tgttagtgct cctattgctt
      181 caatagtacc aattttcact attcctttta ttgcaagata gcctgttaaa aaaacgagag
      241 atatctgaaa aaaaatattg agaaagaagc taatagcgcc tgctaacgtt tctacagttg
      301 tctttctttg tataaccatc tttaataaaa ttcctgcttc tttaattttc ttaggcaata
      361 catataaaag attcaaggac gctaacacat caaatccatt caatatagtc tcactagatt
      421 ttaaaaaagc ttcattttgg ttagttaaat ttagactaac ttctcgcatt ttcgatgcaa
      481 agatttttgg tacaagtagc ataatcatta atgaaaacaa ggtggctaca gtcaatgacc
      541 aatgatagtg attaagagtc acaactgcaa atatagtacc agaaattcct tttattacta
      601 aaaaaagttg tttaaaagcc tgatcattta aagtctgaac atcattattt agccacgaaa
      661 gatatgttcc tgatgattta ctatgaaatt cttgataggt agagttagag atgtctgtgg
      721 caactctatt tcgaatctct agattaaact cttggatcac ttcaacctga taatttttca
      781 ctacccagtc aaggaatatt atcccacacc agacaatcat ttggtagatt gacaatttca
      841 aaaaccgctc taaattcatc gcaattaatt cattcaacac cagagcatta atagttgctg
      901 cataaattag caataattga ccagcaacaa taaatatcgt taataaacta aattttttta
      961 tatt
//
LOCUS       DAVMAF010000173          923 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVMAF010000173 DAVMAF010000000
VERSION     DAVMAF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.174"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            184..387
                     /locus_tag="VYO97_002122"
     CDS             184..387
                     /locus_tag="VYO97_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV1754850.1"
                     /translation="MNTKMMEQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
     gene            705..908
                     /locus_tag="VYO97_002123"
     CDS             705..908
                     /locus_tag="VYO97_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pncT"
                     /protein_id="HEV1754851.1"
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGCIGWGLLKSIGGMIK
                     PAPYVPPVCIPKSSWNPAPPVPC"
BASE COUNT          321 a          118 c          186 g          298 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta aaaaataatg
      121 atatactaaa cttgtcaaag ttgcaacaag acaaaaatta aaaataaaaa aggagtattt
      181 gtcatgaata caaaaatgat ggaacaattt tctgttatgg ataatgaaat gcttgatagg
      241 atagagggag gaatatttgg agttgacgat gctttgtttt gggcagggtt aggctatgtt
      301 gcaggtagca ttgttgatac agcaattgat gattttacga atcaatgccg gaaaaatcct
      361 catcagtggt tctgtgttcg tgtatagata tagtagtgtt gtattgtata agaatttata
      421 taacttaatg acctatgcag tgatagaaac tattcttttg caaacgagtc gcttattctg
      481 ttataagttt acgtgctcct agtcacagtt cttgtgcttg atagaaagaa tgacacctat
      541 aaaaatgcga gatattggct atatcactca cttattcacc tccccgtgat ttgtagtagt
      601 gataggtttt ctcactatta ttataaacat tataaaataa agagtacaac actttttcat
      661 tctgtgttgt gccttgagta aaacgaaagg aatgaattat aacaatggaa aaaattgatt
      721 atattacttt gaatgaagta gaattagaga cgattagtgg tggagatgac tgttttattg
      781 gagatatcgg atgtatcggc tgggggcttt tgaagagtat cggagggatg ataaaacctg
      841 ctccatatgt tcctccagta tgcattccaa aaagttcgtg gaatcctgca cctccagttc
      901 catgttaaaa agttattgta aac
//
LOCUS       DAVMAF010000174          869 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVMAF010000174 DAVMAF010000000
VERSION     DAVMAF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.175"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            17..730
                     /locus_tag="VYO97_002124"
     CDS             17..730
                     /locus_tag="VYO97_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1754852.1"
                     /translation="MHKKYSKNKLGFNNWIFSNYQITDEVKVLELGCGTGELWKSNSD
                     SIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDIQKISFENETFDIVIANMLLH
                     HVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLASLFKDEVNQDLENRTFTLQNG
                     KRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGISEIGSLEEEIIRKRLESEFN
                     NGMLIIPKEYGMFIARKES"
     gene            743..>869
                     /locus_tag="VYO97_002125"
     CDS             743..>869
                     /locus_tag="VYO97_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1754853.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEI"
BASE COUNT          323 a           85 c          176 g          285 t
ORIGIN      
        1 tgatattcga gtagaattgc ataagaagta ttcaaaaaat aaattgggat ttaataattg
       61 gatattttct aactaccaaa tcactgatga agtaaaggtt ctagaattgg gatgtggtac
      121 aggagaattg tggaaaagta atagtgattc tatagataaa atgaagcagt tgattgttac
      181 agatttttct aaagatatgg taaaatcaac gaagtcagtg attggaaata gaaacaatgt
      241 caattatgag ataatggata ttcaaaagat ttcttttgag aatgaaactt ttgatattgt
      301 tattgctaat atgcttctac atcatgtgaa tgatattcct aaagctctct ctgaggtgaa
      361 tagggtctta aaaaccggag gcatttttta ctgtgctaca tttggtgaaa atggagttgt
      421 aaattatttg gcaagtttat ttaaagatga ggttaatcaa gatttagaaa atagaacgtt
      481 tactttacaa aatggtaaaa ggtacctgag taggtatttt aactctgtcg atacattact
      541 ctatgatgat gagttacagg taactagtat agatgaccta gttaaataca tccaatcgtt
      601 taaaggaatt tcagaaatag gttcactaga agaggaaata atacgtaaaa gattggaaag
      661 tgagtttaat aatggtatgt tgatcattcc taaagaatat ggtatgttta tcgctcgaaa
      721 agaaagttaa gggaatgaaa gtgtgaaaaa ttacggagaa gcttttcgat attttagaaa
      781 attaaacgga tattctttag agtatgctgc agctgatttt atatcaaaat ctcaactttc
      841 tagatttgaa aggggagaaa atgaaattt
//
LOCUS       DAVMAF010000175          854 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVMAF010000175 DAVMAF010000000
VERSION     DAVMAF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.176"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            81..419
                     /locus_tag="VYO97_002126"
     CDS             81..419
                     /locus_tag="VYO97_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754854.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            394..>854
                     /locus_tag="VYO97_002127"
     CDS             394..>854
                     /locus_tag="VYO97_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1754855.1"
                     /translation="MSELLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERV"
BASE COUNT          269 a          166 c          219 g          200 t
ORIGIN      
        1 agtagtctac ggagaaaaag gcctgtctac gggtcttcta cagcgaatac gcatgcacag
       61 gctagcaagg gtggcaggtc atgtttttga agcagagtag actgaaacct tatccaatgc
      121 gacggtttga aaagactgtc actgaggaag gtgtcgcgaa agaagggtat gccaaggaag
      181 ctgagacagt ccgccttgaa ttgtggccag ctagtagcaa actacaatct gaattgtatg
      241 gcgagcgtgt caatgatatt ttgaacgcaa atgccaacaa atcagctact atcaaagtga
      301 aagatggtgt gtgtatcgat agccagacag aagtgactca cagggttatc tctaaaaagg
      361 tctacacaca tcatcaagtc ttggagttag agcgtgtcag agctactagg ggcagatagg
      421 cttatagcta aatgtagacg attggctagt aaaaaaactg gcgaggatat cgtcttacgt
      481 gcggtacaca atgctactat aaaggttgtc caagcagatg caagaagact cgcaccagcg
      541 agagatggag agcttataac tagtatcaaa actagggcaa aaatggacgg agataaggct
      601 ataggcgagg tttacaccaa cctaaaatac gctccttacg ttgagtttgg gacagggcca
      661 ataggacaag ctagccattc tggtatctct ccagaggtca gcgtaactta caagtctagt
      721 ccgtggtatg tgcatgaaga ccaaatcaat gtaggacctt accactttca aaagattggg
      781 gagttctaca agatgtatgg tcaacctgct cagccttatc tttatccagc tttgagagac
      841 aatcaagagc gtgt
//
LOCUS       DAVMAF010000176          849 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVMAF010000176 DAVMAF010000000
VERSION     DAVMAF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.177"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..263)
                     /locus_tag="VYO97_002128"
     CDS             complement(<1..263)
                     /locus_tag="VYO97_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEV1754856.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPN"
     gene            complement(172..>849)
                     /locus_tag="VYO97_002129"
     CDS             complement(172..>849)
                     /locus_tag="VYO97_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1754857.1"
                     /translation="LADTPEDAKVAMKILSEEGLLELPGDSARAEFLKNTLDESATEI
                     LRTALKEDIYTFSHVPNLTDENFAGNTSGVAMEFKLMGLEMITKTKEANYKRGLRQRI
                     AIFAHYLGMKQIALESHSIVPQFSRGLPKNLLEISQIVNNLEGKVTNRQLISLLPFVE
                     DPDAELEALEEEKKKNMEDMPMFNKDNTKPEDEVEDEESGVLGEEESQSDLPADGQGR
                     KAGRPVR"
BASE COUNT          202 a          215 c          149 g          283 t
ORIGIN      
        1 tcattcggtc tcgcttcaag tactttacga agttcattca gatttttctt gtctttcatg
       61 ttcttcaaga cttgtctagc ttctacctga cttagaccat aatcacgttg gaacttatca
      121 aaaatcttat tgacttcctt atccaagtaa gtcttggctt cctgatagac cttatcgaac
      181 tggtctgcct gcttttcggc cttgtccatc tgctggtaaa tcagattggc tttcctcttc
      241 gcccaatact cctgattctt catcctctac ctcgtcttcg ggtttcgtgt tgtctttgtt
      301 gaacatcggc atgtcttcca tgttcttctt tttctcttct tccaaggctt ccagctcagc
      361 gtcagggtct tccacaaacg gcaagagaga aataagctgt ctattggtca ctttgccttc
      421 caaattgttc acaatctgag agatttctaa caagttctta ggcaaaccac gactgaattg
      481 tggaacgatt gaatgagact ctaaagcaat ctgcttcatg cctaagtaat gagcaaaaat
      541 cgcaatacgc tgacgcaatc ctcgcttata gttcgcttcc ttggtcttag taatcatctc
      601 aaggcccatc agcttaaatt ccatggctac gcctgatgta ttccctgcga aattctcatc
      661 agtcaaatta ggcacatggc taaatgtgta gatgtcctct ttaagagctg tacgcaagat
      721 ttcagtagca ctttcgtcca gcgtattctt caagaactca gcccttgcac tatcgcccgg
      781 caattccaaa agaccttctt cagaaagaat cttcattgct accttagcgt cttctggagt
      841 gtctgctaa
//
LOCUS       DAVMAF010000177          844 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVMAF010000177 DAVMAF010000000
VERSION     DAVMAF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.178"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(23..829)
                     /locus_tag="VYO97_002130"
     CDS             complement(23..829)
                     /locus_tag="VYO97_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1754858.1"
                     /translation="MLAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIGLSF
                     LGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFL
                     ILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMA
                     VGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISS
                     SFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          301 a          167 c          126 g          250 t
ORIGIN      
        1 atacctataa ttaaattttg cattagttat taacccctct atttttcata gttacataaa
       61 taagtactgg acccccgatt attgcagtaa ttatccctac ttcaatttca cctggtttac
      121 ctaacatacg gccgattata tcacatataa gcaagagctc tgcacctata aaagatgaag
      181 aaatggtcat tgtgcgtata tctttgctta taaataagcc acaaaagtga ggaactataa
      241 gacctacgaa gccaataggt ccaccaattg cagtaatact tgaacataaa agcacacttg
      301 caattattgc aagtgatctt atcctattaa cattaactcc aagaccaaca gccatttcat
      361 cacccatagc taaagcgttt aaatctgatg aaataaatat agctatcaag tgacctaaaa
      421 ttataaaagg tagtagtgta gatatagaag ataatgtagc tgctccaagg ctacctattt
      481 gccaaaatct aaatttgtct aagacgttat tattcggtaa aattaaaaaa cttacaaaac
      541 tgcttaaagc catactaaca caagttcctg ataaggctag ttttataggg gtaaggcctg
      601 cttttccgct tacagcaatc gcgtatacaa aaattgcact tactaagcca ccaatgattg
      661 caaaacttat atggcttatg cttgatgaaa ttcctaaaaa agaaagacca attactacac
      721 taagacttgc tcctgtgttt ataccgagta tacctggatc agctattggg tttctagtaa
      781 ctgattgcat caatacaccg cttatggcaa gactagaacc tgctaaaatt gcaaaaatag
      841 ttct
//
LOCUS       DAVMAF010000178          828 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVMAF010000178 DAVMAF010000000
VERSION     DAVMAF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.179"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..205
                     /locus_tag="VYO97_002131"
     CDS             <1..205
                     /locus_tag="VYO97_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEV1754859.1"
                     /translation="VVEKDGINFEMQYHTQESFDLKNGSLHELYEKYRDTNTSDLERM
                     KLFKEMLDLSNGLEIPKNIERVK"
     gene            207..416
                     /locus_tag="VYO97_002132"
     CDS             207..416
                     /locus_tag="VYO97_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754860.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            413..826
                     /locus_tag="VYO97_002133"
     CDS             413..826
                     /locus_tag="VYO97_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1754861.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
BASE COUNT          297 a          117 c          169 g          245 t
ORIGIN      
        1 agtcgttgaa aaagatggta tcaactttga aatgcagtat catactcagg aaagtttcga
       61 cttaaaaaat ggttcattac atgaactcta tgagaagtat cgtgatacga atacatctga
      121 tctagaacgc atgaaattat ttaaggaaat gcttgattta agcaatgggc ttgagattcc
      181 taaaaatata gagagggtga agtgatatga aagatattaa atactaccgc acaacgacga
      241 acaatgctca agtacttcgt ttgattgatg gtgtcatgca agtttttgac attgaaaaaa
      301 agtgggttaa tagcatggat tggtttaata aaatcttttt taatgacttt acggattttg
      361 aagaaatttc agaaaatgat gcatttactt atattgacag gatggtagcg gcatgattga
      421 tattgccttg gctatcgcta aaaaagcaca tgcagggcag gtagataaag cgggtgttga
      481 ttacatacag catcctctct atgtggccag tcaagtcaac actgaacaag aaaaagctgt
      541 cgctctttta catgatgtga ttgaggatag cgatataact gctgccgatt tattcgcgtc
      601 tggcttgtca aatgaagttg ttacagcggt acaaattttg acaaagaaaa aaggtcaaag
      661 ttatcaagaa tatcttggga aagtaaaatc aaataattta gcaagagttg taaaacttgc
      721 agatttgaaa cataactcag atttatcacg tttgaaatct gttaccaata cagactacga
      781 gcgtgttaaa aaatataaaa atgcaattta ttacttaagc acctagag
//
LOCUS       DAVMAF010000179          814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVMAF010000179 DAVMAF010000000
VERSION     DAVMAF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.180"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..642
                     /locus_tag="VYO97_002134"
     CDS             <1..642
                     /locus_tag="VYO97_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1754862.1"
                     /translation="GQYLFNPHNTPDNFIFLHAGLGIYTSFFTKEKIGASKNPYIGEI
                     GHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFKNSQLTVLKSLVKTEKDIHLD
                     TLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPADKIYINSQLLNYQPFTHEVRD
                     KIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVAFFIEHSNVLQDIISP"
BASE COUNT          280 a          160 c          110 g          264 t
ORIGIN      
        1 ggacaatacc tttttaatcc acacaatacc cccgataact ttattttcct acacgctgga
       61 ttaggtattt acacttcctt tttcacaaaa gaaaaaatag gagcctctaa aaatccttat
      121 atcggagaaa ttggacacac cattgtcgaa ttgaatgggc aatattgtga atgcggaaaa
      181 aaaggttgtt tacaaacata tatttcggat gcttggttaa tcaaacacgc ccaattatta
      241 tttaaaaatt cccaactaac tgtactaaaa agccttgtaa agactgaaaa agacattcat
      301 ttagacaccc ttttaacggc ttataattta ggcgactccg ctttacgcca acaaattgat
      361 aaaggagtca atttattggc cacttctatt gcaaatcttc tcctcatcaa tcctgctgat
      421 aaaatctata tcaacagtca attgcttaat tatcaacctt tcactcatga agtcagggat
      481 aaaatccaag accagctcca cttcgttccc tttactcgta atatagaaat tgaaatttta
      541 ccttacaaca aacatcgtgg aagtatagga gcttgtgcat tagctatcgt tgcttttttc
      601 atagagcata gcaatgtatt acaagatatt atttcacctt aatatatatt agaaatctat
      661 acacctgttt aaatcaacta taacctgtag tagatatctc gtatttagac aatatgaaaa
      721 caagacgact tccatatagg aaaccgcctt ctcgctatgt tgagtgattt atattaaaat
      781 aacttttctt ctagctgcat tttattatta taaa
//
LOCUS       DAVMAF010000180          811 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVMAF010000180 DAVMAF010000000
VERSION     DAVMAF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.181"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            122..811
                     /locus_tag="VYO97_002135"
                     /pseudo
     CDS             122..811
                     /locus_tag="VYO97_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          286 a          143 c          166 g          216 t
ORIGIN      
        1 ctaaagttgt acaagaaaag tgcaaataag aaatctccag attaggaact atccgtgagt
       61 tctctagtct ggagattttt caatagactt cctgcgaaac aaaatatggt ataatagttc
      121 tatgaattat gaagcaagta aacaactaac cgatgcacga tttaagcgtc ttgttggtgt
      181 tcaacgcacg acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
      241 cgcaaaaggt gggcgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca
      301 atatgtgcga gaatatcgaa cttatgaaga aattgcggct gatcttggta ttcacgaaag
      361 caacttaatc cgtcggagcc aatgggttga agtaactctt gttcaaaggt ggtgttgcga
      421 tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg gaagtaaaaa
      481 tcaatcgacc taaaaaaaga attagcgaat tattctggta aaaagaaatt tcacgctatg
      541 aagactcaag cgattgtcac aagtcaaggg agaattattt ctttggatat cgctgtgaac
      601 tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atatcggaca agctggtaaa
      661 atcttggctg acagtgatta tcaagggctc atgaagatat atcctcaagc acaaattcca
      721 cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aaacctataa ccatgcacta
      781 tctaaggata gaagcaaggt tgagaacatc t
//
LOCUS       DAVMAF010000181          810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVMAF010000181 DAVMAF010000000
VERSION     DAVMAF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.182"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            26..>810
                     /locus_tag="VYO97_002136"
     CDS             26..>810
                     /locus_tag="VYO97_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1754863.1"
                     /translation="MGQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHE"
BASE COUNT          260 a          174 c          132 g          244 t
ORIGIN      
        1 caaacaactc attagaaaga ttcatatggg acaattacat tttatcacaa aactactcga
       61 tatcaaagac acaaatatcc aaattataga tgtcgtcaat agggattcac acaaagaaat
      121 catcgccaaa ctggactacg acgccccatc ttgccctgat tgtggaagtc taatgaagaa
      181 atatgacttt caaaaaccgt ctaagatccc ttacctcgaa acaactggta tgccttctag
      241 aattctcctt agaaaacgcc gtttcaagtg ctatcactgt tcaaaaatga tggttgctga
      301 gactcccctg gtaaagaaaa atcaccaaat ccctcgtatc atcaaccaaa aaattgctca
      361 aaagttaatt gaaaaaattt ctatgactga tattgcccat cagctggcca tttcaacttc
      421 aactgtcatt cgcaagctca atgactttca ctttgagcat gatttttctc ggcttccaaa
      481 gattatgtct tgggatgagt atgccttcac taagggaaag atgagtttca ttgcgcaaga
      541 ttttgataat cttaatatta tcactgttct tgaaggcaga acacaagctg tcatccgaaa
      601 tcactttctt cgctacgata gagccgttcg ttgtcaagtg aaaatcatta cgatggatat
      661 gtttagtcct tactatgact tggctaaaca gctttttccg tgtgctaaaa tcgttctaga
      721 tcgtttccat attatccaac atctcagccg tgccatgagt cgttttcgtg ttcaaattat
      781 gaatcagttt gaacgaaaat ctcatgaata
//
LOCUS       DAVMAF010000182          753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVMAF010000182 DAVMAF010000000
VERSION     DAVMAF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.183"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..625
                     /locus_tag="VYO97_002137"
     CDS             <1..625
                     /locus_tag="VYO97_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1754864.1"
                     /translation="VYNGVEIDEDFNENQINVLLPINKYVIGMIGRISEQKNPFFFVE
                     FAKKLSEIYSNLYFVIVGDGELRGRTEELIEEYGLRSSFFITGWVDNPEDYLAQFNQA
                     VLFSKWEGFGLAVAEYMKHKKPILITNVDGMSELVIDGESGFKVPLYNLEVTVDRSRS
                     IIENRELANELGSAAFQRVRSTFEIKEKVSELENIFMSLREDDNVNI"
BASE COUNT          260 a           85 c          163 g          245 t
ORIGIN      
        1 agtgtataat ggagtagaga ttgatgaaga ttttaacgaa aatcaaataa acgttttatt
       61 acccataaat aaatatgtta ttgggatgat tggtcgtatt agtgaacaga aaaatccttt
      121 cttttttgtt gaatttgcaa aaaaattatc agagatttat agcaatttat attttgttat
      181 tgtcggcgat ggcgaattgc gtgggcgaac tgaagaacta attgaagagt atgggcttcg
      241 aagctcattt tttataacag ggtgggtgga taatccagag gattatttag ctcagttcaa
      301 tcaggcagtt cttttctcga aatgggaggg ctttggattg gcggttgcgg aatatatgaa
      361 acataagaag ccaattctta taactaatgt tgatgggatg tcagaattgg ttattgatgg
      421 tgagtcaggt tttaaagtcc cactatataa tttagaagta actgtagata gaagtagaag
      481 tattattgag aatagagaac tagccaatga gttaggtagt gctgctttcc aaagagttcg
      541 atctacattt gaaataaaag aaaaagtgtc agagttagag aatatattca tgagtttaag
      601 agaggatgat aatgtcaata tataaacttt gtaaagatat tgaaagaaaa actatgtcgc
      661 ctgctaaaaa agcaatggct aaaaacgact attttgcatt ttatgttgga agacctttat
      721 cctatctttt aacagttcct ttcgtgaaaa caa
//
LOCUS       DAVMAF010000183          710 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVMAF010000183 DAVMAF010000000
VERSION     DAVMAF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.184"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            72..698
                     /locus_tag="VYO97_002138"
     CDS             72..698
                     /locus_tag="VYO97_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyl transferase"
                     /protein_id="HEV1754865.1"
                     /translation="MVYSDMSTIDASNNLIDISINKIMGIELPNINNLYFIQAYIWGC
                     TAGFNHALLEMVPSVDIDKDYLYIEKLAHDSYFAKFALEYGKVLFCPEQLVLYRRHGH
                     NVTTSHHFKLSPLNVFRKAILGFNELALTHARVYNQTLYMLKKASGKNPLSDRLLEIQ
                     EVIKIGGLKGVRYFYQNRISRKQLVRTIGLYTIMLFGAYKKYIMKELL"
BASE COUNT          247 a           93 c          135 g          235 t
ORIGIN      
        1 aagatgatat ttggaaagag cacaagttag aaatacagct gttaagattt tctaaagatg
       61 acatgccaga gatggtttac tctgatatgt caacgattga tgccagtaat aatttgatag
      121 atattagtat aaataaaata atggggattg aattaccgaa cataaataat ttgtatttta
      181 ttcaagccta tatctggggg tgtactgcag gttttaatca tgcattgcta gagatggttc
      241 cttcagttga tattgataaa gattatttat atatagaaaa actggctcat gatagttatt
      301 ttgcaaagtt tgcgctagag tatgggaagg tgttgttctg ccctgaacaa ctggtcttgt
      361 atcgaagaca tggacataat gtaacaacta gtcatcattt taaattatct ccgctaaatg
      421 ttttcagaaa ggctatattg ggtttcaatg aattggcact tacacatgct agggtatata
      481 atcaaactct ttatatgcta aaaaaagctt ctggaaaaaa tcctttaagt gatagactac
      541 ttgaaattca ggaagtaatc aaaattggag gattaaaagg tgtgagatat ttctatcaga
      601 atcgaatttc tcgaaagcaa ctcgtaagaa caatcggctt atataccatc atgctttttg
      661 gggcctataa aaaatatatt atgaaagagc tcttataatg cttttaaatt
//
LOCUS       DAVMAF010000184          674 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVMAF010000184 DAVMAF010000000
VERSION     DAVMAF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.185"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..593)
                     /locus_tag="VYO97_002139"
     CDS             complement(<1..593)
                     /locus_tag="VYO97_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1754866.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFQYVSDSSMVGYMGKTKELKMYQEEL"
BASE COUNT          229 a          136 c           81 g          228 t
ORIGIN      
        1 tcaagttcct cttgatacat ttttaattct ttagtttttc ccatataacc aaccatacta
       61 ctatcactta catattggaa gtcctcataa tatattactt tataacgcat aaattcaagc
      121 gcccttccaa tactattcac aaaaacatga tcaacatggt caccaagtga aagcggacaa
      181 tatacgacac atttgtcgtc taaatgcatt aacagctctt ttatgatatc attctttaat
      241 gtgtcctcat tttttaattc actatagata tgacggtata gaaaattgcc atttctatct
      301 ttcctataga gacattcata gtacgataag tgtctaaaat cacattgtag acgttcacaa
      361 gctaacctgt cttctttctt cctttcttca atcggatatt tcccaaggtt acacaactta
      421 tgaaattgct tagcagaggg ctgtagctgt tggctcaaag ggtaaccaga aaatatagta
      481 ataacaagta caatttctcc ttctgaagtt aattttgaaa tataatcacc acaggaaaaa
      541 attgcgtcat ctaaatgtgg agataaaaag atatacttag tattgttact cataaccatt
      601 ccctctacaa tttatctaaa aactcactaa gtgtctgatt aaattccaca tcatcaaaaa
      661 aattcacctt attc
//
LOCUS       DAVMAF010000185          647 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVMAF010000185 DAVMAF010000000
VERSION     DAVMAF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.186"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(9..647)
                     /locus_tag="VYO97_002140"
                     /pseudo
     CDS             complement(9..647)
                     /locus_tag="VYO97_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          199 a          117 c          111 g          220 t
ORIGIN      
        1 tgctatagtc aattgaaaca agaacaggac aaaagagcct cgtaaaaggt attgcaactt
       61 ggtaatacct ttttgaggtg ctttttgata tgagcccatg ttttctcaat aggattgtac
      121 tcaggcgagt agggaggaag aggtaaaagt ttatgcccaa actcttcgca taaaagttct
      181 agcttcccca ttctatggaa tcttgcatta tccataatat agtagattga aactagaata
      241 gtacacatct acttctaaaa cattgttaga attcgatttt actatcctga tcgatttatc
      301 ctattcttat tttattttac tataataacc gatggtgtgg ttaatgttgg taagagaaac
      361 ttctgaaacc aagcttcaaa aaagtcgctc gtcatcgtct cttcgtaagt cattggagcg
      421 attaattcac catttgttag acctgcaacc aaagaaatcc tctgatatct tcttccagat
      481 actttgcctc ttattaactg accttttaat gagcgaccat attctcgata aaaataagta
      541 tcaaatcctg tttcgtcaat ctaaacaggt gctaggtgct ttaaactatt aaaattctta
      601 agaaataagg ctactttttc tggctcttgt tcatagtagg tgtggtt
//
LOCUS       DAVMAF010000186          611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVMAF010000186 DAVMAF010000000
VERSION     DAVMAF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.187"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            1..607
                     /locus_tag="VYO97_002141"
                     /pseudo
     CDS             1..607
                     /locus_tag="VYO97_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          210 a           54 c          137 g          210 t
ORIGIN      
        1 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
       61 aaatcatttg ttatgttgga aggttggatc cagaaaaaag gccggataga ttccttgaat
      121 ttgcagaaaa attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta
      181 gtatgtgggc tgctttgaaa gaaaaaattt gctatttgaa atgtagagat aatttcaggt
      241 tacttggtga aatctatcca gtactatagt ttaccaaata tcggatttat tgtatattcc
      301 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt
      361 tttagctagt aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt
      421 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
      481 tgagtatttg aaatatatag gtgagaatag taaatcaaaa ataagaaaac atttttctgt
      541 gcaaaaaatg tttgtagaaa ccatgagagt atatgatgaa ttattagaga agagtagtca
      601 tggataggaa t
//
LOCUS       DAVMAF010000187          611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVMAF010000187 DAVMAF010000000
VERSION     DAVMAF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.188"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..>611)
                     /locus_tag="VYO97_002142"
     CDS             complement(<1..>611)
                     /locus_tag="VYO97_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1754867.1"
                     /translation="ADLDSPAYSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDL
                     MKDSYYKATFDLQQQTGLAYGFSGLPEDEIKHLQSFSWVGDGSTYSTNIWKNTGKLTS
                     SIKDELLISLMTGRDTRETAQAIAERFNVGQNDARRLVRTESAFFHNQMELLNYEEAD
                     IEKYIFVAVLDKRTSRICQEHDNQVYDRDKAVPGVNCPPMHPW"
BASE COUNT          155 a          149 c          126 g          181 t
ORIGIN      
        1 caccaagggt gcataggcgg acaattgaca ccagggacag ccttatccct atcatagacc
       61 tgattgtcat gctcctgaca aatgcgtgat gtacgcttgt ctaagacggc cacaaagata
      121 tacttctcta tatccgcttc ctcatagttg agtagctcca tttggttatg aaaaaaggct
      181 gattctgtcc gaaccaaacg ccttgcatcg ttctgcccca cattgaaccg ctcagcaatt
      241 gcttgtgcag tttctcgtgt atctcggcct gtcataaggc ttatgagtaa ttcatctttt
      301 atgcttgatg taagcttccc tgtattcttc cagatgtttg ttgagtacgt acttccatct
      361 cctacccaac taaaagactg tagatgttta atctcgtcct caggaagccc agaaaagcca
      421 tatgctagtc ctgtctgctg ttgtaggtca aaggtagcct tgtagtaact atccttcatc
      481 aggtcgctat aaaaggcatc tgagcctgtc ttttctgaat gatagataga ttcacgcata
      541 cggtctaaat cgtcgctcaa acgctctagg cgcttcatac ggaaagaata agctgggctg
      601 tctaagtcag c
//
LOCUS       DAVMAF010000188          611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVMAF010000188 DAVMAF010000000
VERSION     DAVMAF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.189"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..>611)
                     /locus_tag="VYO97_002143"
     CDS             complement(<1..>611)
                     /locus_tag="VYO97_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1754868.1"
                     /translation="ADLDSPAYSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDL
                     MKDSYYKATFDLQQQTGLAYGFSGLPENEIKHLQSFSWVGDGSTYSTDIWKNTGKLTS
                     SIKDELLMSLMTGRDTRETAQAIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEAD
                     IEKYIFVAVLDKRTSRICQEHDNQVYDRDKAVPGVNCPPMHPW"
BASE COUNT          158 a          147 c          126 g          180 t
ORIGIN      
        1 caccacggat gcataggtgg acaattgacg ccagggacag ccttgtccct atcatagacc
       61 tgattgtcat gctcctgaca aatccgtgat gtacgcttgt ctaagacggc cacaaagata
      121 tacttttcta tgtctgcttc ttcatagctg agtagttcca tttggttatg aaaaaaggct
      181 gattctgtcc gaaccaaacg tcttgcatcg ttctgaccta cattgaacct ctcagcaatt
      241 gcttgtgcag tttctcgtgt atctcggcct gtcatgaggc tcataagtag ttcatctttt
      301 atgctagaag taagcttccc cgtattcttc cagatgtctg tagagtaggt acttccgtca
      361 cctacccaac tgaaagactg tagatgttta atctcgttct caggaagccc agaaaagcca
      421 tatgctagtc ctgtctgctg ctgcaggtca aaggtagcct tgtagtaact atccttcatc
      481 aggtcgctat aaaaggcgtc tgagcctgtc ttttctgaat gatagataga ttcacgcata
      541 cggtctaaat catcactcaa acgttctaga cgcttcatac ggaaagaata agccggacta
      601 tctaagtcag c
//
LOCUS       DAVMAF010000189          605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVMAF010000189 DAVMAF010000000
VERSION     DAVMAF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.190"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            46..249
                     /locus_tag="VYO97_002144"
     CDS             46..249
                     /locus_tag="VYO97_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754869.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMITSNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            252..602
                     /locus_tag="VYO97_002145"
     CDS             252..602
                     /locus_tag="VYO97_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754870.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
BASE COUNT          221 a          111 c          131 g          142 t
ORIGIN      
        1 ctcttgaaaa tagaaaacga acacacagat tggagaactt agaaaatgaa cacagaacag
       61 cttaaccaag ccttacaaat gacaattcgt gaaatgtcaa caacttcaac agattcgatg
      121 attacaagta atttcttgag tattcagttg aatgagcaaa gggaagagaa tcaaagactt
      181 caagcacgag tggatgagct ggaagctctg cttgatgaac aaactaaacc agcagacaaa
      241 ggagaataga catggcagaa acaattcaaa acacagataa cttactagac cttacaaaaa
      301 tcacagaacc atttgatctt gcgagtgctt tgcgctacat gaaagaaaat ggagagttca
      361 ttcgttgcaa gaatgtaagc gatgacttct atatgtatcg tgacgttcaa aaacgtcctg
      421 tgatcgtaaa tggccgtcgc caattcaagg atgttgaaac cgtttgggcg ttcaatcagt
      481 ggggtggtac aatcgcaaca atcaacgtag ccgttctgtt gaatcatgaa ttctatatca
      541 tgaaatttga tgcagagggc aatcctgact ggacggttcc aacggtagaa cctaaagaat
      601 aggag
//
LOCUS       DAVMAF010000190          590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVMAF010000190 DAVMAF010000000
VERSION     DAVMAF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.191"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
BASE COUNT          198 a           79 c          116 g          197 t
ORIGIN      
        1 cactgtttga gttatctgcg gtaatctttc tagcttgtct ttgatttttg ttgttattta
       61 taaggtaaaa gaagctggac aaaaagtctt caaaatcgga aaaaggcagc ctatcgggtg
      121 ttcaaaaatc ttgataggat gtcctttatt atggaaagcc ttattggatt ttctcctcag
      181 attgagtttt tgatcagctt tatgagatag gttttgctag agatgtagcc catcatgtta
      241 tttttatgga cggtgggaaa attgttgaaa aaaataatgc ccatcaattc tttagtcgtc
      301 caagagaaga acgaaccaag caattttgga acgaattctt tcgaatgcga tctatatagt
      361 aaaatgaaac aagaacagga caaatcgatc aggacagtca aagcgatttc taaaaatgtt
      421 ttagaagtag aggtgtacta ttctagtttc aatctactat ataactgaaa aattagataa
      481 attagttttg gaaaatgact aaccaaaaga tatccaaagt agtctaaaat tgtctatact
      541 ttatgagtgt tttagttagg aaaaaggctt gttgtctata attggctgca
//
LOCUS       DAVMAF010000191          580 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVMAF010000191 DAVMAF010000000
VERSION     DAVMAF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.192"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..293)
                     /locus_tag="VYO97_002146"
     CDS             complement(<1..293)
                     /locus_tag="VYO97_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pblB"
                     /protein_id="HEV1754871.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFIN"
     gene            complement(298..>580)
                     /locus_tag="VYO97_002147"
     CDS             complement(298..>580)
                     /locus_tag="VYO97_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754872.1"
                     /translation="IDGETGRNANGDMVRDRITTKRKLDCEWGMMTQGEISQLLHAVS
                     SKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEKFKPWSGAKFNLVER"
BASE COUNT          153 a          148 c          113 g          166 t
ORIGIN      
        1 tcgttgatga agtattcccc catcttgacc cactggtagg tatccccaac cagtagacca
       61 atctcagggt gcagggtatc cagcttattg aacgtggtaa tgatactggt aaaggtaatt
      121 ttaccgctac cagcgcaggt tccaccaggc ttataagtat cgcccttgat gtagccatac
      181 tcaaaactag cctctttgat atcccgtgaa gcatattcac caacacgaat agccagcgtc
      241 ctttccttgg caaacatggc tctgtcaaat tgtcgtctag ttaaagcgtc cattttctta
      301 cctctctacc agattaaatt tagcgccaga ccaaggttta aacttctcag taaaggtata
      361 gctaggagct gtcctatcac cgacatagaa agtctttgtg acttggccat ccatggggtc
      421 tggataagat acctcaaaaa atttagatga tacagcatgt aaaagctgac ttatttctcc
      481 ctgagtcatc ataccccatt cacagtctag tttgcgtttg gtcgtgatac ggtcacgcac
      541 catgtcgcca ttggcattac gccctgtctc tccatcgata
//
LOCUS       DAVMAF010000192          573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVMAF010000192 DAVMAF010000000
VERSION     DAVMAF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.193"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            344..529
                     /locus_tag="VYO97_002148"
     CDS             344..529
                     /locus_tag="VYO97_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754873.1"
                     /translation="MKKKGGMHVDNDWNGLAELFANLITKYAGVLDLDNLPDPTPAKN
                     QEMKNSFDMAKTQIETD"
BASE COUNT          211 a           79 c          126 g          157 t
ORIGIN      
        1 ataactctcg ataaattttg aaccttaaca attgaataga ccaagacaag acgttaatta
       61 tttttggagc gtaataacct atccgtcaag atctttcggc tgaataattc ggcaaaaaaa
      121 gttctgctaa gcaaaaagaa agtgagcgat aagtaagagt ttgaatatag ggagggatac
      181 cctatccgct atgaagaaaa tgaggataat gatacttctt tagtgaaagc cggctcatct
      241 taacaggggc ggtggtgaga ttccaatgtt gctgatccta agcacccgtt aatgtcatga
      301 aacttagtta gatgagaaag ttatagattg attatataag agtttgaaga aaaaaggggg
      361 gatgcatgtg gataatgact ggaatggact ggctgagttg tttgcgaatc taattacaaa
      421 gtatgctggt gttttagatt tggataatct gccagatcca acgccagcaa aaaatcaaga
      481 gatgaaaaat agttttgaca tggcaaaaac acaaattgag acggactaat aaaagtgtta
      541 taatatactt gaaatgatag tccaaactta aat
//
LOCUS       DAVMAF010000193          569 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVMAF010000193 DAVMAF010000000
VERSION     DAVMAF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.194"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>569
                     /locus_tag="VYO97_002149"
     CDS             <1..>569
                     /locus_tag="VYO97_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1754874.1"
                     /translation="NLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIG
                     LEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAK
                     IILKDLCLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSNMIKIS
                     ELINKYKTMDKIIFIVSHDIEFLNEVADE"
BASE COUNT          221 a           68 c          106 g          174 t
ORIGIN      
        1 aaatcttagc tacaaattta ctgatgatga gtgtttaagc ttaaaagata tttcattcaa
       61 gcttgggaaa atttatggca taataggatc caacggacga ggaaaatcaa cgcttttaag
      121 atgtttaata ggtcttgaga aaaaatcaaa agaagaaatt tattttaagg gagagaagct
      181 atctaaaaaa gaaagactca aaaactcttc acttgttatg caagatgtaa atcatcaatt
      241 attcacagat gaagtattca acgagcttag attaggagta aagaattttg atgaagaaaa
      301 ggcgaaaatc attttaaaag atttatgcct ggacgaattt attgaaaggc atccgatgag
      361 tttatcagga gggcaaaagc aaaggcttgc aatagcatct gttatgtgca agaattctcc
      421 atttgtcttt tttgacgaac cttcaagtgg tatggattat tccaatatga taaaaatatc
      481 tgaactgatt aataagtata aaaccatgga taaaataatt tttattgttt cccatgatat
      541 agaattttta aatgaagtgg cagatgaaa
//
LOCUS       DAVMAF010000194          566 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVMAF010000194 DAVMAF010000000
VERSION     DAVMAF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.195"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>566
                     /locus_tag="VYO97_002150"
     CDS             <1..>566
                     /locus_tag="VYO97_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV1754875.1"
                     /translation="LQGRSFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKY
                     WGRFVDQLDERDSNGQVNINYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAG
                     ANNAYNAVLDVSEKLDELGITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYV
                     GELDDYTVYKVPSKFLKGVNALATAPGVV"
BASE COUNT          174 a          103 c          131 g          158 t
ORIGIN      
        1 ttgcaaggtc gctcttttac agtagcaact ggtaacacaa cagagttgaa agactacaaa
       61 cgtaacaaag acaacgaatt tgatcacgtt gaagttgaag aaaaggttta tacccttgat
      121 gaagaaaaat actggggtcg tttcgtagat caattggacg aacgtgactc taatggtcaa
      181 gtgaatatca attatgttat tgcccgtcag gctgcagaag tagtcgctcc atatcttgat
      241 gaactacgtt ttggtgcagc acttggaaac gtaagtgaca atgttgccat gggtaaaaca
      301 gcaggagcga acaacgctta taatgcggtt cttgatgtgt ctgagaaact tgatgagctt
      361 ggaattacaa aagaacgctt gctcttcgtc actccaagtt tctacaaagc gatcaagtct
      421 gaaatcgttc gtctaccaca tggtgacgca gataagaaag tccttggaaa aggatatgtt
      481 ggtgaattgg atgattacac agtctataag gttccttcta aattcctgaa aggtgttaat
      541 gcccttgcta ctgctccagg tgttgt
//
LOCUS       DAVMAF010000195          565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVMAF010000195 DAVMAF010000000
VERSION     DAVMAF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.196"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>565
                     /locus_tag="VYO97_002151"
     CDS             <1..>565
                     /locus_tag="VYO97_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754876.1"
                     /translation="VGKYSVSGPNLIKNSDFKNATNEWGSTQNLGRLVKHSFYHNGQK
                     DLMRLSNATKNENFLYSHRFNLERNTDYVLNFRGFNNSALASYDVYILGRRAGESDGF
                     TIVKKVVSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNGSSSGTADLYITEVDLYKG
                     YKPRTWQPHPEDAVADANKKLEATQTKM"
BASE COUNT          201 a           92 c          118 g          154 t
ORIGIN      
        1 gttgggaaat atagtgtaag cggccctaac ctcattaaga atagtgattt taaaaatgct
       61 acgaatgaat ggggctcaac tcaaaattta ggaagattgg ttaagcatag cttttatcac
      121 aacgggcaga aagaccttat gcgtttaagt aatgcaacta aaaacgaaaa ctttttgtat
      181 agtcaccgtt ttaatcttga acgaaatact gactatgtac tgaattttag aggatttaac
      241 aacagtgctc tcgcaagcta tgatgtttat attttgggac gaagagcagg cgagagcgat
      301 ggattcacaa tcgttaagaa agttgttagc agcaagaaac tatctacctc tagatgcgaa
      361 gatgtctcag taacttttaa ttccggagaa atggataatg cttacattcg ttttgataac
      421 aatggctcat catcaggaac agctgatttg tatattacag aagttgactt gtacaaaggt
      481 tataaaccta gaacatggca accacatcca gaagatgcag tcgcagatgc gaataagaag
      541 cttgaagcaa cgcaaacaaa aatga
//
LOCUS       DAVMAF010000196          508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVMAF010000196 DAVMAF010000000
VERSION     DAVMAF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.197"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>508
                     /locus_tag="VYO97_002152"
     CDS             <1..>508
                     /locus_tag="VYO97_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754877.1"
                     /translation="ASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEF
                     PDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVS
                     QGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWV
                     SISDMPISG"
BASE COUNT          198 a           90 c           83 g          137 t
ORIGIN      
        1 gcttctattc ttatccctct ccccccacta tccgaacaac aacgcataat agaagcaatc
       61 gaatcagctt tagaaaaagt agatgaatat gctgaaagtt ataatagact agaacagcta
      121 gataaagaat ttccagataa actaaaaaaa tctattcttc aatatgctat gcaaggaaaa
      181 ttagttgaac aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa aatacgagca
      241 gaaaaacaaa aactctttga agaaggcaag attaaaaaga aagatttgga catttctatt
      301 gtttcccaag gagatgataa ctcttattat gggaacaaag acgaaacaac ctcttatcct
      361 atatatgaaa tcccagaagc atggagatat attaaatttg ctagcctagt taacttcaga
      421 ataggaaaaa ctcctccacg cagtgaagct actttttggg gaactgaaat accttgggta
      481 tctatatcag atatgcctat atctggtt
//
LOCUS       DAVMAF010000197          492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVMAF010000197 DAVMAF010000000
VERSION     DAVMAF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.198"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>492
                     /locus_tag="VYO97_002153"
     CDS             <1..>492
                     /locus_tag="VYO97_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754878.1"
                     /translation="LNQKLSQAEFEVRAGSIRQEILNVTKDKASKSELTQTAEELSSK
                     IASVQVGGINLLRNTASLLIGDRSKGCWMSASGGNGRAISVEVLDPPKKMIKNMIRVI
                     ENTNGGNKDLTQLVRLRIGEKYTISCYARIASDSPNANVNLLFRSWANNTDLNRKFQK
                     SIS"
BASE COUNT          159 a           83 c          122 g          128 t
ORIGIN      
        1 aactaaatca gaagttgagt caggctgaat ttgaggtgcg agctggctct attcgtcagg
       61 aaatcctgaa cgtaaccaag gataaagcca gcaagtcaga acttacgcag acagctgagg
      121 agctctctag taagatagcg agtgtgcagg tcggaggaat caacctcttg cgtaatactg
      181 cgagtttgtt gattggcgat cgttcaaaag gatgttggat gagtgcaagc ggaggaaatg
      241 ggcgagcgat tagcgtagaa gttttggatc ctcccaaaaa aatgataaaa aacatgattc
      301 gtgttattga aaatacgaat ggtggaaata aagatttaac tcaacttgtt agattgcgaa
      361 ttggtgaaaa gtacacgatt tcttgttatg caaggattgc tagcgatagc ccaaatgcaa
      421 acgtgaactt gctatttcgt tcgtgggcaa ataataccga tttaaatcgc aaatttcaga
      481 aatccatctc tc
//
LOCUS       DAVMAF010000198          476 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVMAF010000198 DAVMAF010000000
VERSION     DAVMAF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.199"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            145..>476
                     /locus_tag="VYO97_002154"
     CDS             145..>476
                     /locus_tag="VYO97_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754879.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSR"
BASE COUNT          141 a          101 c           83 g          151 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggctatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttt
//
LOCUS       DAVMAF010000199          446 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVMAF010000199 DAVMAF010000000
VERSION     DAVMAF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.200"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            complement(<1..289)
                     /locus_tag="VYO97_002155"
     CDS             complement(<1..289)
                     /locus_tag="VYO97_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754880.1"
                     /translation="MAGYVAMVLTYFGAGIVAAHLTDSDIYIILGALSGISLIFPRLV
                     MHKYINTVAQDESVSSIKDKSDFNTIKILALNMTSITGIPQVLLLLTILTNQ"
BASE COUNT          153 a           94 c           63 g          136 t
ORIGIN      
        1 actgatttgt taaaatagtt aatagcagta aaacctgcgg aattcctgta attgatgtca
       61 tgtttagagc cagtattttt atagtattaa aatcggattt atctttaatg ctactcacag
      121 actcatcttg agctactgta ttgatatact tatgcatcac taaccttgga aaaatcaatg
      181 aaatcccaga taatgcaccc aggattatat agatatctga gtcggttaaa tgagcagcta
      241 ctattcctgc accgaaatac gtcaacacca tggctacata gccagccatt gcatctacta
      301 cacttccatc cttcgagtat tgctcccgat atctggctaa gttcccatct actccatcta
      361 gtaagttcca taaaaaaaat agaaaccatg ccagtaataa tacaacgaaa tcagttgtaa
      421 atatcattat tataaatcca acaatc
//
LOCUS       DAVMAF010000200          420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVMAF010000200 DAVMAF010000000
VERSION     DAVMAF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.201"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>420
                     /locus_tag="VYO97_002156"
     CDS             <1..>420
                     /locus_tag="VYO97_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEV1754881.1"
                     /translation="DEDADYSKLKRRARNPETGKVEYVPADMTYKEWYSKYVAKDGEK
                     VYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKLAGLEFRKKTVESLSRK
                     IIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEY"
BASE COUNT          158 a           62 c          100 g          100 t
ORIGIN      
        1 gatgaggacg cagactacag caagttgaag cgcagagcaa ggaatccaga gacaggtaaa
       61 gttgagtacg tgcctgccga tatgacttat aaagagtggt atagcaagta tgttgcgaaa
      121 gacggggaaa aggtgtataa tcaagataca agagaagcca aggcgaaatt ttatagcgaa
      181 caactattgt ccaaaatttc aggagttgag ccaaaaatta caagtgatat gcaacgtatc
      241 gcaggagaaa acaaattggc aggtcttgaa tttaggaaga aaacagttga gtcattatca
      301 cgtaaaatta ttgcagatag cctagttgaa aatataagtt tgtcaaaagc cgtgagtaag
      361 attaatgacg ccttaaggta cacaactatt ttcgattccg atacttttac agaagagtat
//
LOCUS       DAVMAF010000201          412 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVMAF010000201 DAVMAF010000000
VERSION     DAVMAF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.202"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>412
                     /locus_tag="VYO97_002157"
     CDS             <1..>412
                     /locus_tag="VYO97_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754882.1"
                     /translation="LNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRA
                     HGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFATPK
                     LYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQD"
BASE COUNT          113 a           97 c           74 g          128 t
ORIGIN      
        1 ctcaaccttg aattggtcga attcttttta cagtttcacc agctaaacca actcattgta
       61 gatatcgatt ctacccattt cacaacttat ggcaagcaag aaggtgttgc ttataacgcc
      121 cactatcgtg ctcatggcta tcatcctctt tatgctttcg aggggaagac aggttattgt
      181 ttcaatgccc agcttcgtcc tggtaatcgt tattgttctg aagaggcaga cagctttatc
      241 acacctgttt tagaacggtt taatcaactt ctctttcgaa tggatagtgg ctttgcgacc
      301 ccaaaattat acgatttaat tgaaaaaaca gggcaatact acctcataaa actcaagaaa
      361 aatactgttc tgagccgtct tggagacctt tccctccctt gcccacagga tg
//
LOCUS       DAVMAF010000202          402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVMAF010000202 DAVMAF010000000
VERSION     DAVMAF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.203"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
BASE COUNT          149 a           84 c           46 g          123 t
ORIGIN      
        1 caatcactag tccaataatt ggactagtga ttggatataa taaaaataac aaaaaatgaa
       61 tttcaccaat tcctgcaaaa aaatgtttca tagctatagt tttacgccca taaataccca
      121 tcaaatttac cgtctacgat atctaaacca tatgcaatgg ctcctcctaa tgagaagact
      181 tcagcaacag tagccgcaat attaagagca ttagtaatag ttgccatact aactccacca
      241 atatatcttg ttataacact tagttaccta gataagatat cctttagctt gcttccaaaa
      301 tttaagtctg cacctataat atgagttcat attagtactg ctaaatccta tctatagtaa
      361 tgtcaaacac ctaaataaag tcatctctcc aattcattca ag
//
LOCUS       DAVMAF010000203          397 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVMAF010000203 DAVMAF010000000
VERSION     DAVMAF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.204"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..>397
                     /locus_tag="VYO97_002158"
     CDS             <1..>397
                     /locus_tag="VYO97_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1754883.1"
                     /translation="KCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQ
                     VQTMLEQAFTEKYYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMME
                     SFFGILKSEMFYGYEKTFKSLNQLEQAIVD"
BASE COUNT          137 a           78 c           74 g          108 t
ORIGIN      
        1 aaagtgctac acagatgtga cagaatttgc cattccagca agtactcaaa agctttactt
       61 atcaccagtt ttagatggct ttaatagcga aattatcgcc tataatcttt caacttcacc
      121 caacttagaa caagtacaaa caatgttgga acaggccttt acagagaaat actatgagaa
      181 tacgattctc catagtgacc aaggctggca ataccaacac gattcttatc atcagtttct
      241 tgagggtaag ggaattcaag catccatgtc acgcaaggga aacagccaag acaacggtat
      301 gatggagtcc ttctttggga ttctgaaatc ggaaatgttt tatggctatg agaaaacatt
      361 taaatcactt aatcaattgg aacaagctat tgtagac
//
LOCUS       DAVMAF010000204          392 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVMAF010000204 DAVMAF010000000
VERSION     DAVMAF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.205"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            19..>392
                     /locus_tag="VYO97_002159"
     CDS             19..>392
                     /locus_tag="VYO97_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1754884.1"
                     /translation="MPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVLE
                     FLVNYYAHSKENGITGVTQKTI"
BASE COUNT          141 a           45 c           83 g          123 t
ORIGIN      
        1 gacgatgatg atgaatatat gccagatcga attttaaagt tgatggcttg ttttaaaaag
       61 agtagaatga agaatttagc tttagtttat agttatggaa taataattta tccaaatggt
      121 acacgagaag aggagaagac cgattttgtt ggaaatccct tgtttgttca aatggttcac
      181 aatatagcag gtacgtcatt ttggttgtgt aaaaaagagg tgctagaatt aattaatggt
      241 tttgagaaaa tagattcaca tcaggacggt gttgttttat taaaactact tgctcaagga
      301 taccaaattg atatagtgct agaattcttg gtgaattact acgctcacag taaagaaaac
      361 ggtatcactg gagtgacaca aaaaacaatt aa
//
LOCUS       DAVMAF010000205          378 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVMAF010000205 DAVMAF010000000
VERSION     DAVMAF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.206"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..363
                     /locus_tag="VYO97_002160"
     CDS             <1..363
                     /locus_tag="VYO97_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphate hydrolase"
                     /protein_id="HEV1754885.1"
                     /translation="ILFSKEPYPSLSDMPMEFRKEFLMEMSILGDAMLEVLNAYRINY
                     DILGNSTPVLHAHLFPRYMWEEEERRKTVVHRYDSSVFSNPEFEFTIEKHGPIIKKLR
                     SAIDRHYREYQQLLDDSN"
BASE COUNT          134 a           51 c           66 g          127 t
ORIGIN      
        1 attctttttt caaaagaacc ctatccttct ttatctgata tgccaatgga atttagaaaa
       61 gaatttttaa tggaaatgag tatattaggt gatgcgatgc ttgaagtgtt aaatgcctat
      121 agaattaatt atgatatctt aggaaattca acacctgtac ttcatgctca tttatttccg
      181 agatacatgt gggaagagga agagagaaga aaaactgtag ttcatagata tgattcttca
      241 gtttttagca atcctgagtt tgaatttact atagaaaagc atggaccaat tattaaaaaa
      301 ctgaggtctg ctattgatag acattataga gagtatcaac aattattaga tgattcaaac
      361 tgaactccac attttcga
//
LOCUS       DAVMAF010000206          363 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVMAF010000206 DAVMAF010000000
VERSION     DAVMAF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.denovo.207"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            <1..172
                     /locus_tag="VYO97_002161"
     CDS             <1..172
                     /locus_tag="VYO97_002161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754886.1"
                     /translation="QDFYDWESNVKRLAGEELEITEERYAELADNIASNGVAISDVLE
                     KILPEPEFLEED"
     gene            174..>363
                     /locus_tag="VYO97_002162"
     CDS             174..>363
                     /locus_tag="VYO97_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1754887.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGER"
BASE COUNT          107 a           73 c           87 g           96 t
ORIGIN      
        1 acaagatttc tatgattggg aatcaaatgt gaaacgactg gcaggagagg aacttgagat
       61 tactgaggag cgctatgctg agctggctga caatattgcc agcaacggtg tcgctatctc
      121 agatgttctt gagaaaatcc tccctgaacc tgagttctta gaagaggatt gatatgtcta
      181 tagagttgct gaagaaatta acaggcgaag aagatactca gcttctcatg ttgctccaaa
      241 caagggctac aaatcttatc ttgtcagaga ctaatcgcac atctttgaca cctgctttaa
      301 gtcttttaat acctgaggtt gctatcgagc tccacaaccg ctcaggagcg gaaggagagc
      361 gtt
//
LOCUS       DAVMAF010000207        11286 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00069708
            SAMN33607392-rid17937423.guided.062, whole genome shotgun sequence.
ACCESSION   DAVMAF010000207 DAVMAF010000000
VERSION     DAVMAF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607392
            Sequence Read Archive: SRR23725468
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721345.1
            Genome Coverage       :: 158x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:53:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,175
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 1,997
            CDSs (with protein)               :: 1,997
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 65 of 129
            Pseudo Genes (incomplete)         :: 67 of 129
            Pseudo Genes (internal stop)      :: 33 of 129
            Pseudo Genes (multiple problems)  :: 32 of 129
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607392-rid17937423.guided.062"
                     /strain="AUSMDU00069708"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST4237"
     gene            424..609
                     /locus_tag="VYO97_002163"
     CDS             424..609
                     /locus_tag="VYO97_002163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754888.1"
                     /translation="MKKKGGMHVDNDWNGLAELFANLITKYAGVLDLDNLPDPTPAKN
                     QEMKNSFDMAKTQIETD"
     gene            701..2380
                     /locus_tag="VYO97_002164"
     CDS             701..2380
                     /locus_tag="VYO97_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1754889.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEDRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            2648..3214
                     /locus_tag="VYO97_002165"
     CDS             2648..3214
                     /locus_tag="VYO97_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754890.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3535..3723
                     /locus_tag="VYO97_002166"
     CDS             3535..3723
                     /locus_tag="VYO97_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1754891.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3785..4543
                     /locus_tag="VYO97_002167"
     CDS             3785..4543
                     /locus_tag="VYO97_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1754892.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            4601..5455
                     /locus_tag="VYO97_002168"
     CDS             4601..5455
                     /locus_tag="VYO97_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1754893.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5567..6451
                     /locus_tag="VYO97_002169"
     CDS             5567..6451
                     /locus_tag="VYO97_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1754894.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6614..8353
                     /locus_tag="VYO97_002170"
     CDS             6614..8353
                     /locus_tag="VYO97_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1754895.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKIPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            8628..8930
                     /locus_tag="VYO97_002171"
     CDS             8628..8930
                     /locus_tag="VYO97_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1754896.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            8986..9413
                     /locus_tag="VYO97_002172"
                     /pseudo
     CDS             8986..9413
                     /locus_tag="VYO97_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9492..10079
                     /locus_tag="VYO97_002173"
     CDS             9492..10079
                     /locus_tag="VYO97_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1754897.1"
                     /translation="MGGNPPMKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            10328..10617
                     /locus_tag="VYO97_002174"
                     /pseudo
     CDS             10328..10617
                     /locus_tag="VYO97_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            10722..11024
                     /locus_tag="VYO97_002175"
                     /pseudo
     CDS             10722..11024
                     /locus_tag="VYO97_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         3989 a         1569 c         2361 g         3367 t
ORIGIN      
        1 gttaagtata agagggtaat ttatagaatt ttgttacatc aagataaata ttttatgatg
       61 gtcaaatttt aaaaagatta ataactctcg ataaattttg aaccttaaca attgaataga
      121 ccaagacaag acgttaatta tttttggagc gtaataacct atccgtcaag atctttcggc
      181 tgaataattc ggcaaaaaaa gttctgctaa gcaaaaagaa agtgagcgat aagtaagagt
      241 ttgaatatag ggagggatac cctatccgct atgaagaaaa tgaggataat gatacttctt
      301 tagtgaaagc cggctcatct taacaggggc ggtggtgaga ttccaatgtt gctgatccta
      361 agcacccgtt aatgtcatga aacttagtta gatgagaaag ttatagattg attatataag
      421 agtttgaaga aaaaaggggg gatgcatgtg gataatgact ggaatggact ggctgagttg
      481 tttgcgaatc taattacaaa gtatgctggt gttttagatt tggataatct gccagatcca
      541 acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac acaaattgag
      601 acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta aattaaaaaa
      661 attatatatg atatagaaaa attatattga gattaatact atgtcaaaag aaaaaataaa
      721 agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt cgttagaggc
      781 acaaaaatca cgaatgaaag cttttgctat ttacaatgat tatgaaattg ttggagagta
      841 tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca atcgaatgat
      901 ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta agttatcaag
      961 atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg attatggagt
     1021 taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga aactaatgat
     1081 ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc aaacaatgga
     1141 agatcgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc cgtatggata
     1201 taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa taagaacgat
     1261 tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat acttagagaa
     1321 tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg atgcaggtct
     1381 tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg gaagaagaac
     1441 tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag atgaatatct
     1501 aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag ctgctcaggt
     1561 taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag atacacgcac
     1621 acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt ttgggaacaa
     1681 gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact atggttgtaa
     1741 acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta gagaagaatt
     1801 gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga aatttgcttc
     1861 tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa aagaaataga
     1921 taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa ttgaggaaat
     1981 agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt tagatgatag
     2041 actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg atgcgaaagc
     2101 aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata aggttctgat
     2161 ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt taatttcagc
     2221 tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat ggctaaaatc
     2281 cattactttt aaactaccaa ttatagaaga aaatctcaac ataggtttgg ataatgatga
     2341 acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag gttccccaaa
     2401 ccttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta cgagttgaag
     2461 catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg aaggtcgata
     2521 aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag atgatacaga
     2581 gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga aaatggagag
     2641 attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt tagtagcatt
     2701 tgattccata gaagatggga aagcctttgt agctcaaatc ccgggataca ctctggaaac
     2761 agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt atatggaaat
     2821 tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg aggaaaatgt
     2881 aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag tgatcgaagg
     2941 atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct atgtcgaaac
     3001 aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat atgaaatcga
     3061 cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa aagggatcga
     3121 agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag atgtagaagg
     3181 atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt gtgagtatat
     3241 acctttgcta gtaaaagagt caaaacgtta taacgaaggg gggaagaaaa cccttgagtg
     3301 taagtctttt tcttgtttag agggaaagta ttagagtagc cgctcataat ttatactaga
     3361 tttgataact gatttgagac agattttatt tctataaata ttgtgaaaat tttttaatac
     3421 caaagattta aagttactgt gttaaaaatc ctctccactt ttaaactaat gtattataaa
     3481 agccagttga cacaagtgtt acacctgtga tagaattaac ttgaggtgat gaaaatggca
     3541 accgataaaa atagaataat gattagcttg gatgataaaa atctagagaa acttgaaaat
     3601 ttagtagaag atgctagaga tagaagggga atgcgactaa caaagtccca ggttattgaa
     3661 ttactcctaa ataccgttga ttatttcgat gatattatgg gggctattta ctcaaaaaaa
     3721 taagagcaaa attgctatta ttttttacaa attggtgtaa cactagtgat ataggagtga
     3781 tgaaatggtt gtttatatta gacaaagtaa attaccaagt gaggtttcaa ttaataaata
     3841 caatgctcaa gtaggtgcct acttacaagg agaagaagct gttttatatc aatctttttc
     3901 tgaaataaaa gagttaacaa gtgaagatat agttgtagat tatataatgg agactagagc
     3961 attactaaaa atgatgggct taaacgttcc ggttcatgac tatcctattg agcttaaaga
     4021 gttttatggt cgaaagattt atgctggtat tttaggagag attgtgaata tacctgataa
     4081 ttggggaaaa tttattaagc ctaaagctgg ttcaaaagtc tttactggaa gagttgttaa
     4141 tggaacccat gatttaatag gtattggtct acctttcgac tatcctatat ggattagtga
     4201 ggttgtagaa ttcatagctg aatggcgttg ttttgtgtta gatggtcgcg tattagatgt
     4261 tcgaccctat acaggtgatt atcatgcaca atttgatgca agtgtaattg atgaagcgat
     4321 atcatgttgg aaagatgcgc caatagctta tggactagat atcggtgtta ctcgcgatgg
     4381 cagaacactt gttgttgaag taaatgatgg ttatgcattg ggaaattatg gcctatctcc
     4441 tttaaaatca atcaattttc atagagctag gtggaaagaa atggtaaaac cctattttga
     4501 aaaaaatgaa atttttaaaa tccaacagga tgttgttttc taaaataaat atggtatata
     4561 accaatataa aaagcatcaa aatgaacaag gaggaatctg atgcttggag caattattgg
     4621 agatattgtc ggttctgttt acgaatggaa caatatcaaa acaaaggact ttcctttatt
     4681 tcggaaggac tgctttttta cagatgacac ggttatgacc tgtgctgttg cagaagcaat
     4741 tatgaatggt ggacaaaaag acgatttcat tgacgcgatg aagaaatatg gcagaatgta
     4801 tccgaatgct gattacggtg ctcggtttaa tgcatggcta aacagcgata accgtgagcc
     4861 ttataatagc tttgggaatg gatcagctat gcgtatttct ccatgtgctt ggatcatgga
     4921 ctgtggtttt tatgcgaaaa ctggtatgtg gccatcatct agaggactta cgagtctttc
     4981 tgcagaggta actcataatc atccagaagg tgtcaaaggc gctatggcta cagctgatgc
     5041 tatctttcta tgtcgttttt actttggtgg ttattgtaga gagtacgagc aatcaattaa
     5101 cgacaatcct acagagtgta aaagacgaat taaggattat atagaaaaag aatacgatta
     5161 caatctatct caaactttag atgaaatccg tcctaactat cgttttaacg aaacatgtca
     5221 ggaaactgtc cctcaagcca ttatcgcctt tcttgaaagt agagacttcg aagatgcgat
     5281 aagaaatgcc atctcacttg gtggcgacag tgatactctc gcagcaatca cttgtagcat
     5341 agcagaggca gcttacggta ttcctgattg gatcaaggat aaggcctatt cttacttgga
     5401 tgaaccctta aaggatgtag ttaggcgatg ggaaaataga ataaaagcgt attaatctcc
     5461 agtacgtcag atttctcttt tctgtatttt cattgtataa taggattaca tttacttgca
     5521 tacgacgaaa tgtaaacatt tctgtttaaa attaaggata aaatcaatga aaaaagcaat
     5581 ggtaattatc aaccctactt ctggtggcga gaaggctttg gattacaaag aaaagctgga
     5641 gaataaagca aaagaatact ttgaatatgt tgaaaccaaa attaccgaaa aagcgctgga
     5701 tgcaacacat tttgctgaag aagcttctcg tgagcagtat gatgcagtgg ttgtgtttgg
     5761 tggagatgga actgtcaatg aagtcatttc aggtattgat gagagagact acattcctaa
     5821 gttagggatt atcccaggcg gtacgggtaa cctcattaca aaacttttgg aaatcaatca
     5881 agacatcgat ggcgcaattg acgaactgga ttttgattta accaataaga ttgatatcgg
     5941 taaagcaaat gacaactatt ttggttatat ctttagtatc ggttctctgc ctgaggcgat
     6001 tcacaatgtt gaaatagagg acaaaacaaa attcggtatt ttaacctatg ctgtaaatac
     6061 catgaagtct gtcatgacag atcaggtctt taacattaag gttgagacag aaaatggaaa
     6121 ttatgttggt gaagctagcc atgttttggt ccttttgaca aattacttcg ctgataagaa
     6181 aatctttgaa gaaaacaagg acggctatgc caacattttg attctgaaag atgcctctat
     6241 attctccaaa ttatccgtca ttcctgattt attaaaaggg gatgttgtcg caaatgataa
     6301 tatcgagtat atcaaagcgc gtaatattaa aatctcttca gatagtgaat tggagtcaga
     6361 tgttgacgga gataaatcag ataacctacc tgtagaaatc aaagtcctag ctcagcgagt
     6421 agaagtattt tcaaaaccga aagaggatta gtatatagag aaagcctttt ttaaggcttt
     6481 ttgtatactt taaaagatag ttcctttaac aacggacatt ccttgcaaat agttcacaaa
     6541 aatagtatac tggatttatt gagtttgaaa acgtttgcgt aaaatttgaa tgaatacttt
     6601 aggagacaaa ttgatggaat tgagtgctat ttaccatagg cctgagtcgg agtatgccta
     6661 tctttataag gataagaaac tccatattcg aattcgaact aagaaagggg acattgaaag
     6721 catcaacttg cactatgggg acccttttat ctttatggag gagttttatc aggatacaaa
     6781 agaaatggtc aagataactt ctggtacctt atttgaccat tggcaggttg aagtgtcagt
     6841 tgactttgca cgtatccagt atctctttga gctcagagat acagaaggtc aaaatatttt
     6901 gtatggcgat aaagggtgtg tggaaaattc tctagaaaat cttcatgcaa ttgggaatgg
     6961 atttaagttg ccttatcttc atgagattga tgcctgcaag attcctgact gggtttcaaa
     7021 tacggtatgg tatcagatat ttcctgaaag atttgccaat ggcaatgctc tattaaaccc
     7081 agaagggact ttagactggg attcatctgt cacacctaag agcgatgatt tctttggtgg
     7141 tgatttacag gggattattg atcatatgga ttacttgcaa gacttgggta ttactggact
     7201 atatctttgt cccatctttg aatctacaag caatcacaag tacaatacga cagattactt
     7261 tgaaattgac cgtcattttg gagacaagga gacctttcgg gaactggtgg atcaagcgca
     7321 tcatcgtggc atgaaagtca tgctggatgc ggtatttaat catattggtt cgcaatctct
     7381 tcaatggaaa aatgtcgtca aaaatggtga acagtctgct tataaggatt ggttccatat
     7441 tcaacaattc ccagtgacaa ctgaaaagct agttaataag agagacttac cctatcatgt
     7501 ttttggtttc gaggactata tgcctaagct aaatacagcc aatccagagg tcaagaatta
     7561 tcttttaaag gttgcgactt attggattga agagtttaat atcgatgctt ggcgtttgga
     7621 tgtggctaat gagattgacc atcagttctg gaaggatttt cgtaaggcag ttttagctaa
     7681 aaatcctgat ctttatatcc taggagaagt ctggcataca tctcagcctt ggctaaatgg
     7741 agatgagttc catgccgtca tgaattatcc tttatctgat agtatcaagg actatttctt
     7801 acgaggaatt aagaagacag accagttcat cgatgaaatc aatggagagt ctatgtatta
     7861 caagcagcag atttcagagg tcatgtttaa tctcttggat tcacatgata cagagcgaat
     7921 cctgtggacg gccaatgaag atgttcaact ggttaaatca gccttagcct ttctcttttt
     7981 acaaaaagga acaccgtgca tttattacgg aaccgagtta gccttgactg gaggaccaga
     8041 tccagattgt cgtcgttgta tgccttggga acgtgtatca agtgacaatg atatgctgaa
     8101 ctttatgaag aggctgatta agattcggaa atacgcgtca gtaatcattt cgcatggcaa
     8161 gtatagcctt caagaaatca aatctgatct agtagctctg gaatggaaat acgaaggacg
     8221 gatcctcaaa gcaatattca accaatcaac agaagattat cttttagaga aagaagcagt
     8281 agcactagca agcaattgcc aagaattgga gaatcagctt gtcatctctc cagatggatt
     8341 tgtgattttc taaaaactag ttgatgaaga ttatggtaca tttcatatct tatatagtat
     8401 aataaggcta gttactaaac ttgtaaagga gaacttaaat gaattgtaga ggacatgaaa
     8461 caagacaaag aattgttaga gattttgaag tttagcctaa agcacatatt aagctgttag
     8521 caaatcaaca aaaacatagt gatgcaggag caactattga agatgaatat tatgtattta
     8581 tcgctgagag taaaattgat gacaagaagg aagttattca gtgttgcatg ggtgcggcaa
     8641 gggatttttt agaactaatt aatcacaaag ggctacctct ttttaatccg cttgtaggtg
     8701 attctcatgt aaataataga caagaatatg acaatacagg gagtggaaat ttacaacctg
     8761 aaaagtggaa tgaaactgca aagcagcttt ataatgctat aatgtggttg attattttat
     8821 ggaatgctaa gccggataca cctttattta attttaaaga cgaagtaatt aagtataaaa
     8881 catatgagcc ttttgaaagc agtataaaat tggtatcaat caatgagtaa caaagtatca
     8941 gcatgattcg aaacatttac ttgtaatttg cggaggaaca tcaatatgga tggaataaca
     9001 aaagatttta taaaaacggc taaactaatg aaacaattat ggccccaatt gaccgataaa
     9061 gaagctattg atgaagtaaa aaatatacga atggcaaaaa tactgcaatc tttactgaag
     9121 ttgaaggtga cacaattgta ggtctagcac tatgttcact cagatttgat tatgttgaag
     9181 gttgtaaata tagtcctgtt ggattcttag aagggattat tgttgacgag gaatatcgtt
     9241 taaaggatat tgctaaaaat ctctgtacaa aatgtgagga atgtgcgaaa aataaaggat
     9301 gtaaggaatt tgcaagtgac tgtactttaa cgaatacgga ttctataaga tttcatctca
     9361 atattggatt tcaggaggca aatagaatta ttcattttaa gaaaaaatta taatttagaa
     9421 acgtgaataa acttgtttaa actttatgta tctttagaaa gtgtgttaac ttgaaagaac
     9481 aaattttttt gatgggtggg aatcccccaa tgaagaaata tagtattgtt gataaaattg
     9541 tattatcaac caagattaaa agaattatca tttttacagt ttttcgagaa aattgggaac
     9601 cgtacatgaa aaagtacacg gaagtttttc aaagtcaatt tcctaatcta aacattgatt
     9661 acttgctcct ggacactgag cagattgatc ttgatagcta tctagatgct gacataatta
     9721 tcatcggtgg tggaaatacg gaaaaatata ttgctactta tgttaatcag gagttcaaaa
     9781 gttatatcga tcatatgctt aataaagagg caaaaattat aggattttct gcaggagccc
     9841 tactattagg agaaaaagtc tatgtctcac ctaatgataa ttcagatcat cagataaaga
     9901 taaaaaatgg attaggactc tttagtcagt ttttaattag tgtccattat gattcctgga
     9961 atgataaagc aaataaggat agagctgaag aactcgttaa tgttcccata attccactaa
    10021 atgatcattc ttgtcttgta ttggataaac ttggaaacat catagagaaa attgactaac
    10081 tttcataatg taaaaatgga tctaggaaat aaaagtgtag acgaactgga taggttacta
    10141 gtgaaaactg caaaaagtgt tgtaaagtga atcggagtgt gcttgatgga ttttagtagt
    10201 aagatagcca taaataaagg ctggtcagat gataaaaaat attgtgtgac agatcaaaat
    10261 cagtaaaaat atttcttgcg tgtttctgat aagtcgaagt tagattataa aaaaatttga
    10321 atttgatatg atggagaaag tagcttctct tggagttccg atgtgtaaac caattagcat
    10381 tgaactctgc gatgacgaag tacattcttt acacgaatgg atagacggaa gagatgcaat
    10441 agatagcatt ttagcttatt cagaaaacca acaatacaca tatggtgtag aagcaggaaa
    10501 gatacaaaga tacttagaaa gattcataca attcctgcta cagaagtttg tgaagattgg
    10561 gaaatttttt ttaatctaaa aattgatgat aaaatctcca acgaaatgat atggtaagtc
    10621 cgaaacgctc agtgtgtaag cttgttgcta aaagcgtaaa aaccttggaa cgaaaggaat
    10681 aatagacttc ctgcgaaaca aaaatacaat acaataaaac tatggatgat gaagcaagta
    10741 aacaattaag cgatagccgt ttcaagatcc ttgtaggtgt tcagcgcacg acttttgaag
    10801 agatgttagc tgtgttaaaa acagcttatc aacgtaaacg cgcaaaaggt ggacgaaaaa
    10861 gcaaattaag cctagacgat ctccttatgg taactattca atacatgcga gaatagagca
    10921 cttatgaaca aattgcggct gattttggca ttcacgaaag caacttaatt cgtcggagtc
    10981 aatgggttga agcaactctt attcaaaatg gttttacgat ttcaaattct gccttaattc
    11041 tgtaaaaaca gtaaaattcg aaggattgta aggtaagagt ttttttcttt ctgaaaaaat
    11101 ggtataatag caatcaaaac tagaaaataa aacggaattt ggaacagatt tgtctgtatc
    11161 ctagtagagt ggtgatacta tgaagattag taagaggcac ttattaaatt attccatctt
    11221 gattccctac ttgcttttat ctattttggg cttgattgtg gtctattcga ccaccagtgc
    11281 tatttt
//
